LOCUS       DATBRX010000001         6276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3574910, whole genome shotgun sequence.
ACCESSION   DATBRX010000001 DATBRX010000000
VERSION     DATBRX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3574910"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VIF08_00005"
     CDS             <1..931
                     /locus_tag="VIF08_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134394.1"
                     /translation="GVAIVESLEAGKSSTIDINITTSNQYGYALSERIFGSSFPRDAA
                     TARAVYTRRAVIDQLFNYGSPISADTPLLLAWRTGSVVDVDLQGESPNRIGDALYMIS
                     LGVSLDANQVFADQAMKRTIISTDAAQGWGDMGSMYLSRGSMVVENRPISFSGTFHAT
                     LLEVALSQGELIALSGKGTILDPLPADEQPDQDDPVEGATGEPTATPDPNATPDPNAT
                     PVPPRDDPVKPGAPNMPFGLPAFQLFDRVSQLWVEFPALDPAEPYRIASPERYVDNSG
                     SVLMRFVNRAEAGQFGEEQVYFQVATRIEGTIE"
     gene            928..1902
                     /locus_tag="VIF08_00010"
     CDS             928..1902
                     /locus_tag="VIF08_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8134395.1"
                     /translation="MTTAVHTSGLTKRFGRSTAVAGVQLDVEAGEIFGLVGPNGAGKT
                     TTLKMLATLLPPTTGDAEVGGHSVRNNPDGVRGVIGYMPDTFGVYDDMRVWEYLDFFG
                     RCYGMTAARRRQMIGDLLELVDLADKKGDYVQDLSRGMQQRLCLAHALVHDPQVLLLD
                     EPASGLDPRARVELRELLRELRALGKTILISSHILPELEELCTSVAIIDHGRVLASGR
                     VEDIADRFRVGGVYRATVIGSDEKVGSAEQVFAGLSDVVSVVRRPGGLLELALRGDEN
                     TAATVLSAAIRGGVKLSSFAPAASDLEELFLQITDLSSDKSTTTEVSA"
     gene            1899..3242
                     /locus_tag="VIF08_00015"
     CDS             1899..3242
                     /locus_tag="VIF08_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8134396.1"
                     /translation="MTALAAAIAQRGGRIGGRVGHALGRFWSGISAVSIKELRGRMRG
                     RRAFIVLTIYLMLLGLFAFAIYVYLKQQAATGSSIGGFPQPDTGFPGWPSGQTLTNGT
                     ALSAGIGHGIFGGLLFLETLLVLVLAPAFTTGAISLEREKQTLDLLVTTPLSTLGMVI
                     GKLFSALVYVFLLIIASIPLASLVFVFGAVGPDDLVRAYVFLFALAFGMGALGLFVSA
                     LVKRTQTATVITFVLVLVLSIGTAAAHQFWQVAARSSSSSSGLLLTSRAGRAPEALLW
                     FNPFVSDLDMVCATAPDTYHESCAYISSVTGRPYFGSPNTNSSPPIFRGEPGVVVPMP
                     APGGVDDAAGLGGVRFDPNAVAEVDCQFPDAKGNCAGGTGENASQALSFGFPRDTFWP
                     KSSAAFVGLGVILTLLAAQLVSPTRRLRISRPKFRRPQPPKSIEADTPTAVENAS"
     gene            3239..5071
                     /locus_tag="VIF08_00020"
     CDS             3239..5071
                     /locus_tag="VIF08_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134397.1"
                     /translation="MNLDPQLLEMRRSLGRFRRRLWLRRIVRDGSFILAGVVVAELGL
                     AVLARLMPFEWQATSAMAVIALGVVALLIDAIRVRPTLSEAALAVDSEDGLADRVSTA
                     LSLATTSPDLASDTPVADEGTDSEPATYARLVRLQRRDALAHLTSADPRGLRIPVPRR
                     QTATSALAALLLVPALLLPNGQNDILNLREQQRAAAETQAQHLNETADRLNEGRTAPD
                     PRVDIAQELRELAQQLRDHPEDLQANLARLGSLEDALRSRIDPANEQRAAAITSLSRS
                     LSRSATGTDSNQSGDPEKTKQDLADLADRLGDMTQEEREQLARDLSQQDATARQAGGN
                     AQTALSDAVRAIQSGDQQAAADALNRLGDALSNGQQQVDLNRDIARAASDLQQTRRDL
                     ANAGQQGQQGQQGQQGQGQGQGQGQGQQPGQSPGQGQGQGQGQGQGQGQGQGQGQGQG
                     QGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGQGSLGGGGSNARYLGSGNGGNSGF
                     QGPTTGNRQFGTGNLDDIFADFGRLGRPGDPSYIPGVGGDGQIQQGQGQGQGTDNQSV
                     IPYTNVFQDFYDFAITSLDRSYVPLDVKDYVRDYFSSLNPTPGQ"
     gene            5084..6106
                     /locus_tag="VIF08_00025"
     CDS             5084..6106
                     /locus_tag="VIF08_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8134398.1"
                     /translation="MAQRTLETATTKPAELSVEEFADRARAIEAEIKSVIVGQNDLVR
                     QTLICLIANGHALLEGVPGLGKTMLVKTIADVIDCSFNRIQFTPDLMPADIVGTNILV
                     EENGQRQFRFQPGPVFANLLLADEINRATPKTQSALLEAMQEQQVSVARERHKLEPPF
                     FVLATQNPLEMEGTYPLPEAQLDRFLFKLWVPFPSEEDLVEIMERTTGAKTPTAGHAA
                     NAKEVVQMQRLARSVPIAPHVTAYAVSVLAATHPDAPRAPELVKQYVRYGGSPRGAQA
                     LVLTGKIHALLEGRYNVAVDDIRAVALPALRHRVIRNFEGEAEGITSDAIVRAVLDAV
                     PPPSVD"
BASE COUNT         1028 a         2263 c         2023 g          962 t
ORIGIN      
        1 cggagtcgcc atcgtcgaga gcctcgaggc gggcaaaagc agcacgatcg acatcaacat
       61 caccacgagc aaccagtacg gctacgcgct gtcggagcgc atcttcggct cgagcttccc
      121 gcgcgacgcg gccacggcgc gggccgtcta cacccgccgc gcggtgatcg accagctgtt
      181 caactacggc agcccgatct cggcggatac gccgctgctc ctggcatggc gcaccggatc
      241 ggtggtcgat gtggatctac aaggcgagag ccccaaccgg attggcgacg cgctgtacat
      301 gatctcgctc ggcgtctcac tcgacgccaa ccaggtcttc gccgaccagg cgatgaagcg
      361 caccatcatt tccaccgacg ccgcccaagg ctggggcgac atgggcagca tgtacctgtc
      421 gcgcggctcc atggtcgtcg agaaccggcc catctcattc agcggcacgt tccacgcgac
      481 gctgctcgag gtcgcgctgt cgcagggcga gctcatcgcg ctcagcggca agggcactat
      541 cctcgaccca ctgccggccg atgagcagcc cgaccaggac gacccggtcg aaggcgccac
      601 cggtgaaccg actgccacgc ccgatcccaa cgcgacaccc gatccgaacg cgacacccgt
      661 tccgccgcgg gatgacccgg tgaagccggg tgcgccgaat atgccgttcg gcctgccggc
      721 attccagctg ttcgaccgcg tcagccagct gtgggtcgag ttcccggcgt tggacccggc
      781 ggagccgtac cgcatcgcca gtcccgaacg gtacgtcgac aattcgggca gcgtgctgat
      841 gcgcttcgtc aaccgggccg aggccggcca gttcggtgag gagcaggtgt acttccaggt
      901 cgccacccgg atcgagggca cgatcgaatg acaacggccg tccacaccag tggcttgacc
      961 aagcgcttcg gccggagcac ggccgtcgcc ggggtgcagc tcgacgtcga ggcgggcgag
     1021 atcttcggct tggtcggacc caacggcgcg ggcaagacga cgaccctcaa gatgctcgct
     1081 acgctgctgc cgccgacgac tggcgatgcc gaggtgggcg gccattcggt gcgcaacaac
     1141 ccggacgggg tgcgcggcgt catcggctac atgcccgaca ccttcggcgt gtacgacgac
     1201 atgcgcgtct gggaatacct cgacttcttc ggccgctgct acggcatgac cgcagcgcgg
     1261 cggcggcaga tgatcggcga cctgctcgag ctggtcgatc tcgccgacaa gaagggtgac
     1321 tacgtgcagg acctgtcgcg tggcatgcag caaaggcttt gcctcgccca cgccctggtc
     1381 cacgacccgc aggtgctgct gctcgacgag cccgcgtcgg gcctcgaccc gcgtgcccgg
     1441 gtcgagctgc gcgagctgtt gcgcgagctg cgtgccctgg gcaagacgat cctgatcagc
     1501 agccacatcc tgcccgagct cgaagagctg tgcacctcgg tcgcgatcat cgaccacggc
     1561 cgcgtcctcg catccggtcg cgtcgaggac atcgccgatc gcttccgcgt cggtggcgtg
     1621 tatcgcgcca cggttattgg cagcgatgag aaggtcggca gcgccgagca ggtgttcgct
     1681 gggctgagtg acgtcgtgtc cgtcgtgcgc cgcccgggcg gcctgctcga gctggcgttg
     1741 cgcggcgacg agaacacggc cgcgaccgta ctttccgcgg cgatccgcgg cggggtcaag
     1801 ctgtccagct tcgcaccggc ggcgagcgat ctcgaggagc tgttcctgca gatcaccgat
     1861 ctctccagtg acaagtcgac caccacggag gtctccgcat gaccgcgctc gccgccgcca
     1921 tcgcgcaacg gggcggccgc atcggcggtc gcgtcggcca cgccctgggc cgcttctggt
     1981 cgggcatcag cgccgtgtcc atcaaggagc tgcgcggccg catgcgcggc cggcgcgcgt
     2041 tcatcgtgct gacgatctac ctgatgctgc tcggcctctt cgcgttcgcg atctacgtct
     2101 atctcaagca gcaggccgcg accggctcga gcattggcgg cttcccgcag cccgacaccg
     2161 gcttccccgg ctggccgagc ggccagacgc tcaccaacgg cacggcgctg tcggccggca
     2221 ttggccatgg catcttcggc gggctgctgt tcctggagac gttgctcgtc ctcgtgctgg
     2281 cgcccgcctt cacgaccggc gcgatctcgc tcgagcgcga gaagcagacg ctcgatctgc
     2341 tcgtgaccac gccgctatcg acgctgggca tggtcatcgg caagctattc agcgcgctgg
     2401 tctacgtctt cctcctgatc atcgcgtcga tcccgctggc gagcctggtc ttcgtctttg
     2461 gcgcagtggg gccggacgat ctcgtgcgcg catacgtctt cctgttcgcg ctcgcgttcg
     2521 gcatgggcgc gctggggttg ttcgtgtcag cactcgtcaa gcgcacccag accgcgaccg
     2581 tcatcacgtt cgtgctggtg ctggtgctgt caatcgggac cgcagccgcc caccagttct
     2641 ggcaggtcgc ggcacgttcg tcgtcctcgt cgagcggcct cctgctcacg tcgcgcgcgg
     2701 gccgcgcccc ggaggcgctg ctgtggttca acccattcgt gtccgacctc gacatggtgt
     2761 gcgcgaccgc gcccgacacc tatcacgagt catgtgccta catcagcagc gtcaccggcc
     2821 gaccgtactt cggctcgccc aacacaaact cctccccgcc catcttccgg ggcgagcccg
     2881 gcgtcgtggt gccgatgccg gcgccgggcg gcgtggacga tgccgcgggc cttggcggtg
     2941 tccggttcga cccgaacgcc gttgccgagg tggactgcca attcccggac gcgaagggca
     3001 actgcgccgg tggaaccggc gaaaacgcca gccaggcgct cagcttcggc ttcccgcgcg
     3061 acaccttctg gcccaagagt tcggccgcct tcgtgggcct gggcgtgatc ctgactttgc
     3121 tggccgcgca acttgtttcg cctacgcggc gtctacgtat ctcgcgcccg aaattccggc
     3181 gcccgcagcc gcccaagtca atagaagctg acacgccaac cgccgtggag aacgcgtcat
     3241 gaacctcgac ccgcaactgc tcgagatgcg ccgctcgctt ggccgtttcc gccgccggtt
     3301 gtggctgcgc cgcatcgtcc gcgacggcag cttcatcctt gccggggtcg tcgtggccga
     3361 gctcggcctt gccgttctcg cccggctgat gcccttcgag tggcaagcca cctcggcgat
     3421 ggcagtcatc gccctgggcg tggtagcgct gctgatcgat gccatccgcg tccgacccac
     3481 cctgtcggaa gcagccctgg cggtcgactc ggaggacgga ctcgctgatc gcgtctcgac
     3541 cgctctctcg ctcgcgacga cctcgccgga tctggcctct gatacgccgg ttgccgacga
     3601 gggcaccgat tctgagcccg ccacctacgc gcgccttgtc cgcctgcagc gccgcgacgc
     3661 cctcgcgcac ctgacaagcg ccgatccacg cggcctgcgc atccccgtgc cgcgccggca
     3721 gacggcaact tccgcgcttg ccgcactgct gcttgtgccg gcgctgctgc tgcccaacgg
     3781 ccagaacgac atcctcaacc tgcgtgagca gcagcgcgcc gcggcagaga cccaggcgca
     3841 gcacctgaac gagacagccg atcggctcaa cgagggccgc acggcaccgg atccgcgcgt
     3901 ggacatcgcc caggagctgc gcgagcttgc ccagcaattg cgcgaccatc ccgaggatct
     3961 ccaggccaac ctcgcccgcc tgggctcgct cgaggatgca ctgcgatcac gcatcgatcc
     4021 cgccaatgag cagcgtgccg cggcgataac ctcgctctcc cgctcgctca gccgcagcgc
     4081 caccggcacc gacagcaacc agtcaggcga tccggaaaag acgaagcagg atctcgctga
     4141 ccttgccgat cgcctcggcg acatgaccca ggaagagcgc gagcagctcg cgcgcgacct
     4201 ctcgcagcag gacgcgaccg cgcgtcaggc cggcggcaac gcgcagaccg cgctgtccga
     4261 cgccgtgcgc gccatccaga gcggcgacca gcaggccgcg gccgatgcgc tcaaccgtct
     4321 gggcgacgcg ctctcgaacg gccagcaaca ggtcgatctc aaccgcgaca tcgcgcgcgc
     4381 cgcatcagat ctgcagcaga cccggcgcga cctggccaat gcaggtcagc agggccagca
     4441 gggccaacag ggccagcagg gccagggcca aggtcagggc cagggtcaag gtcagcagcc
     4501 cggccagagc cccggtcaag ggcaaggtca gggtcagggc cagggccagg gccagggcca
     4561 gggccagggt caaggccagg gtcaaggtca gggtcagggc cagggccagg gccagggtca
     4621 aggtcaaggc cagggtcaag gtcaaggcca gggtcaaggc cagggtcaag gccaaggtca
     4681 ggggcagggc cagggctcgc tcggtggcgg cggttcgaac gcccgctacc tgggcagcgg
     4741 caacggcggc aactcgggct tccagggtcc cacgaccggc aaccgccagt tcggcaccgg
     4801 caacctcgac gacatctttg ccgacttcgg tcggctcggc cgccccggcg acccgtctta
     4861 catcccgggc gtcggtggcg acggccagat ccagcagggt cagggccagg gccagggcac
     4921 cgacaaccag tcggtcatcc cgtacaccaa cgtcttccag gacttctacg acttcgcgat
     4981 cacgagcctt gatcggtcgt atgtcccgct cgacgtcaag gactacgtgc gcgactactt
     5041 cagctcgctc aacccgactc caggccagta ggaggggcgt cacatggcgc agcggacgct
     5101 cgagaccgcc accaccaaac cggccgagct gtcggtggag gaattcgccg accgcgcgcg
     5161 agccatcgag gctgagatca agtcggtcat cgtcggccag aacgacctcg tccgccagac
     5221 gctgatctgc ctcatcgcca acggccacgc gctgcttgag ggcgtgcccg gactgggcaa
     5281 gacgatgctc gtcaagacca tcgccgacgt catcgattgc tccttcaacc ggatccagtt
     5341 cacgcccgac ctgatgccgg ccgacatcgt cggcacgaac atcctggtcg aggagaacgg
     5401 ccagcgccaa ttccgcttcc agccgggtcc ggtctttgcc aacctgctgc tcgcggacga
     5461 gatcaaccgc gccacaccca agacgcagag cgccctgctc gaggccatgc aggagcagca
     5521 ggtctcggtc gcgcgggagc gccacaagct cgagccgccg ttcttcgtcc tcgcgacgca
     5581 gaacccgctc gagatggaag gcacgtaccc gctgcctgag gcgcagctcg accgcttcct
     5641 gttcaagctc tgggtgccct tcccttccga ggaagacctg gtcgagatca tggagcgcac
     5701 gaccggcgcg aagacgccga ccgcgggcca tgccgcgaac gcgaaggaag tcgtccagat
     5761 gcagcgcctc gcgcgcagcg tcccgatcgc cccgcacgtc accgcctacg cggtgtccgt
     5821 cctagcggcg actcaccccg acgcgccgcg cgcgccggag ctcgtcaagc aatacgtgcg
     5881 ctacggcggc tcgcctcgtg gcgcgcaggc gctcgtgctg acgggcaaga tccacgcgct
     5941 gctcgagggc cgctacaacg ttgccgtcga cgacattcgg gcggtcgccc tgcccgcgct
     6001 gcgccaccgc gttatccgca acttcgaggg agaggcagag ggcatcacca gcgacgcgat
     6061 cgtgcgcgcg gtgctcgacg ccgttccgcc gcccagcgtc gactagccgt gggcggcccc
     6121 aactcgatta cgggaccctg gcgacccttg tctcgtcgcg tgacaccact atggtcaccg
     6181 cggcgcttaa ggggcactga ggcacccctt caatgcaccc tttcaacgcc gacctgggca
     6241 agccacgctc accggagctc acacggtgcc cgatca
//
LOCUS       DATBRX010000002        11339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_782132, whole genome shotgun sequence.
ACCESSION   DATBRX010000002 DATBRX010000000
VERSION     DATBRX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11339
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782132"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..151)
                     /locus_tag="VIF08_00030"
     tRNA            complement(75..151)
                     /locus_tag="VIF08_00030"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Asp,seq:gtc)
     gene            198..761
                     /locus_tag="VIF08_00035"
     CDS             198..761
                     /locus_tag="VIF08_00035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8134399.1"
                     /translation="MALELVFETHSTTTDNEAGIATGWLPGRLSQLGRAQAAELGERR
                     RHDRIAIVYVSDLARAQETAQIAFADRLELITDARLRECNYGLMNGTPVAALQEKRAA
                     HIHEPWPDGESYLEVVDRTRSLLTDIVARWDGSRVLLIGHSANQWALDHLVLGGDLRQ
                     LVSRGMVWQPGWEYVVDQRHSYTRPSP"
     gene            758..1873
                     /locus_tag="VIF08_00040"
     CDS             758..1873
                     /locus_tag="VIF08_00040"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEY8134400.1"
                     /translation="MTSRPDRLIDLRSDTVTHPSPEMRRAMAEAELGDDVFGDDPTVI
                     ALEERAAELTGKEAALFVASGTMGNLVSIMAQVPRGGEVIAGNGAHSFAHEAANYAVV
                     VGASMKLLPWDERGQMRLDDIRRAWRNPKDVHEPLTSLVMLENTHSDSMAQPLPADYV
                     AAVGKVVHDGGVPLHVDGARIFNAQVALGTPVKELLAPADSATFCLSKGLACPVGSVI
                     VGRKDFIWQARRARKLLGGGMRQVGVLAAAGLVALRDGSDGMVERLADDHVNAHRLAE
                     GIAGMPGIVGLDPARVRTNFVFFELSKPELRMPFLDALERAGTQMIDYPGGDLIRGVT
                     HYGISADDIDRTISGVRQALADVGIAPAPLAAAVAGG"
     gene            1866..3533
                     /locus_tag="VIF08_00045"
     CDS             1866..3533
                     /locus_tag="VIF08_00045"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8134401.1"
                     /translation="MADAIVAPEPQTEADRELGRLAAERFAALMTRYPVYATFLGLHE
                     HDARLGDASREAILADVEQTRRFLGALEAIEPASLSPYWATERELALFVTRRDLFDAE
                     VHRVWERRASASDEIGDGIFLLFARGGRPLAERLEAIAGRLEAAPRYLDEAPSRFGER
                     APVRLWNETELDAAQSMPGLFDEVLSAARRELGESHRQTRRLEKGIGATRVVLDGYAE
                     WLKEQIARSTDDFALGRADYDTLVGLRAFDGLSADDILEVGQEQLAAERAARQEVARE
                     IDPSATELEVVDRVKSQHAADFAGALTEYRTAMAEARQFIVDHELATVPPTEALKVIE
                     TPEYMRGVIPFAAYFSPGKFEPGKPEGIYVVTPSIDGDPRAMREHNSASIYNTGIHEA
                     YPGHHLQLVVANQHPSLIRLLVDAPEFVEGWAMYCELMMREQGFDTAPSHRLMMHTDA
                     IWRACRIILDVRLQRGEIGVDEATDFMVEQTGFERPHANAEVKWYTYRPTYPLSYLLG
                     RQLLLRLRADEQKRLGSSFSLRGFHDALLNQGGLPISFQRRLLAQDA"
     gene            3536..4234
                     /locus_tag="VIF08_00050"
     CDS             3536..4234
                     /locus_tag="VIF08_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEY8134402.1"
                     /translation="MRLVPSIDLAGGRSRLVFWPGASSGTGAPTDRPERIAAAFVAMG
                     APMIHLVDLDGARQGVPVNTAAIAAVARDVAVPLQLAGGVDGPRQIELAFAAGATRVV
                     VPVWIAAESLDGLRECLRVAGDWLSVGLDARDEQMAAYPWPGAAPSLDELAATLAGEG
                     VRRLVVSHWTADDLPRLAAIRTRHDIAVQLAGGVSEPAQIEAAAAAGIDTLILGEALL
                     SGAIEYPQKVAMNG"
     gene            4227..5018
                     /locus_tag="VIF08_00055"
     CDS             4227..5018
                     /locus_tag="VIF08_00055"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8134403.1"
                     /translation="MAKRKHVAQRSRINEGAQRPGFTRRSSLPPPSERRSSAWMWLVL
                     GALGIVGVIVVAAYAGGFIGRPGATPSPSPAPRPSFANLAPPSATPLANPPSAPAGDG
                     TTATIVTDLGTIVIDLYTDSAPVAAQNFINLAEAGFYNGVGFHRIIPDFMIQGGDPQG
                     TGGGGPGYEIRDDPVVGNYSRGQVAMARPANPDGSKKPDSAGSQFFIMVADTPGLASG
                     GYAIFGTVVSGMDIVDQIVLGDTTGPRGDLAANPVIMRSVTIQRP"
     gene            5313..5388
                     /locus_tag="VIF08_00060"
     tRNA            5313..5388
                     /locus_tag="VIF08_00060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5346..5348,aa:Arg,seq:ccg)
     gene            complement(5410..6201)
                     /locus_tag="VIF08_00065"
     CDS             complement(5410..6201)
                     /locus_tag="VIF08_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8134404.1"
                     /translation="MAKQPHGVDDLRHDLVGAIERGEFRLEFQPVVRLGGGTVVGAEA
                     LVRWRHPRRGTVAPGEFVAVAEEAGLLDGIGTFVLDQAARAAATWPRSNDRPGVVSVN
                     VAPSQLRARDAAARTLALIAGAGLEPRQLMVELTGAVSGDMPAVGAALNELRDAGVRI
                     VLDDFGVATSIADLKAVQVDQVKLAREFVAAIDGPAQEQAFALSLINLADVRGVEVVA
                     KGVESREQAWRLAELGCRYGQGFFFSKPLGATGLLAVLRQGTLRG"
     gene            6276..7016
                     /locus_tag="VIF08_00070"
     CDS             6276..7016
                     /locus_tag="VIF08_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEY8134405.1"
                     /translation="MPVLSERAELPWWSKSRVQRKRSLNTDRIVEAGMAVLDRDGLDG
                     LSVRRLGAELGSGATSVYWHVPGKEALLDLLVDRLMGEAADETRHDPGATWRAELAAH
                     SLALRDVLQRHIGGASLLASRMPVGPNGLRFMEGVLSALADAGFGGHQRALAYAALSG
                     YTIGQVVLESRRGPTTPNDQPAGGEQLQRLGGLLQGAPRDRYPNVVESAADLAELTDE
                     EAFSYGLQRMLDGLEAELGPVTPNRGAI"
     gene            complement(6991..7680)
                     /locus_tag="VIF08_00075"
     CDS             complement(6991..7680)
                     /locus_tag="VIF08_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8134406.1"
                     /translation="MTLDQINSLIVECRRCPRLVAWREEAARNPPARFAGQAYWARPL
                     PGFGDPAARLLIVGLAPAAHGGNRTGRIFTGDRSGDFLFAALHRAGFANQPTSVARDD
                     GLRLSDAYIAAVNRCAPPANKPTPTERDNCLPYLAGQIAALARLRLIVCLGAFAWDGT
                     LRALSANGNKTASRTRFGHGARAEVGRYLLIGSFHPSQQNTFTGRLTPGMLDDVFAAA
                     REVIRSRPGSG"
     gene            complement(7677..9191)
                     /locus_tag="VIF08_00080"
     CDS             complement(7677..9191)
                     /locus_tag="VIF08_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8134407.1"
                     /translation="MAELDQRPFPPGDYPVVVVGSGPGGLQTSYFLKRLGIEHAVISA
                     DPSPGGMFRHFPFFQRLLSWTKPYAPVPRDDRWYQWYDWNSLLADEPGARAIMPTLMD
                     GTSSFPSRPEMEANLAAFVEQAGLAVRYDCRWEATRRDGERFVLSTPDGEYRCQAAIF
                     AVGIAEPYLPDTPGFELVSHYVDTRAAATYAGKRLFIVGKENSGFELATGLLQWAQRI
                     VLASPRPAKLSVNTHSLLGVRARYVLPFEDQNLGGGVFILNASIERIERAPGGFRVHC
                     IRSENGAELSVEADEVIAATGFKCPLRDLPDLGVAVFGRSGLPAMTNYYESATVPGIY
                     FAGTIGQGVAGMKKYGLPANSGAVHGARYNARTMVHHLARTHFGVELPRPEVKPDALV
                     DLLLSEATRAPELWNQKSYLARAFSLDSGSGIRDEGVVPLAQFVDEEGPAAVAIAVET
                     DDLGDIHPALYVREVGRPAVETLLASNPLHDFRSVENRAQLTSLLSGATAGAVR"
     gene            complement(9179..9604)
                     /locus_tag="VIF08_00085"
     CDS             complement(9179..9604)
                     /locus_tag="VIF08_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8134408.1"
                     /translation="MAMTAQELVDMLSSLTLFADLTRPQLEQVAHKMTEESFPVGQRI
                     LRQGFTGTGFYVILDGEVAVKIDGEERARLAKGDFFGEMSILLGEPPVADIVTTRQLH
                     VLHLGGPDLEGFLRDHPQVMYRMLKAVALRLRNASRWRS"
     gene            complement(9605..10060)
                     /locus_tag="VIF08_00090"
     CDS             complement(9605..10060)
                     /locus_tag="VIF08_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8134409.1"
                     /translation="MAATDIVRPMALSTQDKAAALAGVPLFAGISAESMERLAAVAGE
                     QEFAAGQFIVRQGQVGTGLYVVLSGSVKVLRGSDVIARLGKGEFFGELSVIDQQPRNA
                     SVEAAESTSVLALASWDLLALLESDPQLSLNMIKGLVMRVRQAGEKLGH"
     gene            complement(10047..10379)
                     /locus_tag="VIF08_00095"
     CDS             complement(10047..10379)
                     /locus_tag="VIF08_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY8134410.1"
                     /translation="MAHGPPRGVTERLGAERLEATVRGYVQGVGFRYFVVRRAKELGL
                     AGWTANEHDGTVHVVAEGSPAALDELTRHLHAGPPGAHVERVDANRLPSVGDLRGFSI
                     RSGDHGGD"
     gene            complement(10352..>11339)
                     /locus_tag="VIF08_00100"
     CDS             complement(10352..>11339)
                     /locus_tag="VIF08_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134411.1"
                     /translation="LAMDLAAGLLCLAADPADRPCGQCAACHKVAHGNHPDLHLLAPE
                     GAGQQIRVAQVQGLASALALLPLEGRFRVAIIESAQRMNIDAQNALLKTLEEPPARVA
                     LILAADDSAGLLPTVVSRCARARLGPVATEPITALLAERGLADAARAAALTRLASGRP
                     GVAIALAAQPEATVVQSRLAAALLDLLHADRRRRLAAPPELLADAAELLRATTGRVDE
                     SETPVPARRSGSSAAKISPAERRAAAAQLLAVWREVARDLAVAARGGTRELRQHELLD
                     ELAVAGKSVGAKQAAQFLDRLEAVSRALDAYANPELAVDALLIAWPTARRAA"
BASE COUNT         1809 a         3901 c         3907 g         1722 t
ORIGIN      
        1 gactcagtca ccgagttgac tcacaaccag cgagagattc gacgaaatcg gcgtgcggcc
       61 gcgaaaacgg ggactggcgg gcccgaccgg attcgaaccg gcgatctcca gcgtgacagg
      121 ctggcatgtt tggccgctac accacgggcc cgggcagcgg tcggtcagca taccagccgc
      181 cctcccatac tggttccatg gcgctcgagt tggtgttcga aacgcacagc accacgaccg
      241 acaacgaggc tggcattgcg acgggctggc tgcccggcag actctcccaa ctgggccgcg
      301 cgcaggccgc tgagctgggc gagcggcggc gccacgaccg catcgccatc gtctacgtct
      361 cagacctcgc gcgagcgcag gagacggcac aaatcgcatt cgcagacagg cttgagctga
      421 tcactgacgc gcgcctgcgc gagtgcaact acggcctgat gaacggcaca cccgtcgcgg
      481 ctctgcagga gaagcgcgcc gcgcatatcc acgagccatg gcccgatggt gagagctacc
      541 tggaagtggt cgatcggacc cgaagcttgc tcacggacat cgtcgctcgt tgggacgggt
      601 cgcgcgtgct cctgattggg cactcggcca accaatgggc gctcgaccac ctggtgctgg
      661 gcggggacct gcgccaactc gtctcacgcg gcatggtctg gcagcccggc tgggaatacg
      721 tggtcgacca gcgccactcc tatactcggc cttcgccatg acgagccgtc ccgaccgcct
      781 catcgacctg cgctccgaca ccgtcacgca cccctcgcct gagatgcgcc gcgcgatggc
      841 ggaggcggag ctgggcgacg acgtcttcgg cgacgacccg acggtcatcg cgctcgagga
      901 acgcgccgcg gagctgaccg gcaaggaggc cgcgctcttc gtcgcaagcg ggacgatggg
      961 caacctcgtg tcgatcatgg cccaagtgcc gcgcggcggt gaggttatcg ccggcaacgg
     1021 cgcgcactca ttcgcgcacg aggcggccaa ctacgcggtc gtggtcggcg cgtcgatgaa
     1081 gctgctgccc tgggacgagc gcggccagat gcgcctcgat gacatccgcc gcgcgtggcg
     1141 caatccaaag gatgtccacg agcccctgac ctcactggtc atgttggaga acacccactc
     1201 cgattcgatg gcgcagccgc tgcccgccga ctacgttgcc gcggtgggca aggtggttca
     1261 tgacggcggc gtgccgctcc atgtcgacgg agcccgcatc ttcaacgcac aggtagccct
     1321 gggcacgccg gtcaaggagc tgctggcgcc ggccgactcg gccaccttct gcctgtccaa
     1381 gggcctggcc tgtccggtcg ggtcggtcat cgtcgggcgg aaggacttca tctggcaggc
     1441 gcgccgggcg cgcaagctgc tcggcggtgg catgcgccag gtgggcgtcc tcgcggcagc
     1501 ggggctggtc gcactgcgcg acgggtcgga cggcatggtc gagcgcctcg ccgacgatca
     1561 cgtcaatgcc caccggctgg cggagggcat cgccggcatg ccgggcatcg tcggcctgga
     1621 cccggcacgc gtccgcacca atttcgtgtt cttcgagttg agcaagcccg agctacgcat
     1681 gccattcctc gacgccctcg agcgagctgg gacgcagatg atcgactacc cgggcggcga
     1741 cctcattcgc ggggtcaccc actacggcat cagcgccgat gacatcgatc gcacgatcag
     1801 cggcgtgcgc caggcactcg cggatgtggg cattgcgccc gcgccccttg ccgctgcagt
     1861 cgccggtggc tgacgcgatc gtcgcgccgg aaccgcaaac cgaggccgac agagaactcg
     1921 gacgtctcgc cgcggagcgc ttcgcggcgc tcatgacgcg ctacccggtg tacgccactt
     1981 ttctgggcct gcacgagcat gacgcgcggc tgggcgatgc ctcacgcgaa gcgatcctgg
     2041 ccgatgtcga gcagacgcgg cgcttcctgg gtgcgctcga agcgatcgaa ccggcatcgc
     2101 tctcgccgta ctgggcgaca gaacgcgagc tggcactctt cgtcacgcgg cgcgatctgt
     2161 ttgacgcgga agtccaccgt gtctgggagc gccgcgcgtc ggcgagcgat gagatcggcg
     2221 acggcatctt cctgctgttc gcgcgcggcg gccggccgct cgctgagcgg ctcgaggcga
     2281 tcgccggacg gctggaggcg gcgccgcgct acctcgacga ggcgcctagc cggtttggcg
     2341 agcgcgcccc ggtgcggctc tggaacgaga cggagctcga cgcggcccag tcgatgccgg
     2401 gcctgttcga cgaggtcctt agcgccgcgc ggcgcgagct gggggagagc cacaggcaga
     2461 cgcgccgtct cgagaagggc atcggcgcca cccgtgttgt ccttgatggc tacgccgagt
     2521 ggctcaagga gcagattgcg cggagcacgg acgactttgc gctgggccgc gcggactacg
     2581 acacgctcgt cggcctgcgt gccttcgacg gcctatctgc ggacgacatc ctcgaggttg
     2641 ggcaggagca gctcgcggcc gaacgcgcgg cccgccagga ggttgcgcgc gagatcgacc
     2701 ccagcgcgac cgagctggag gttgtcgatc gggtcaaatc gcagcatgcg gcggatttcg
     2761 ccggcgcgct caccgagtac cgcaccgcca tggcggaagc gcgccagttc atcgtcgacc
     2821 acgagctcgc gacggtcccg cccaccgagg ccctcaaggt aatcgagacg cccgagtaca
     2881 tgcgcggcgt catcccgttc gcagcgtatt tctcacccgg caagttcgag cccggcaagc
     2941 ctgaggggat ctacgttgtg acgccctcga tcgacggcga tccgcgcgcg atgcgcgagc
     3001 acaacagcgc ctcgatctac aacaccggca tccacgaggc gtaccccggc caccacttgc
     3061 agctcgtcgt cgccaaccag catcccagcc tcatccggct gctggtcgat gcgcccgagt
     3121 tcgtagaggg ctgggcgatg tactgcgagc tgatgatgcg cgaacagggc ttcgatacgg
     3181 ccccgtccca ccggctgatg atgcataccg atgccatctg gcgggcgtgc cggatcatcc
     3241 tcgacgtgcg gctccagcgc ggcgagatcg gggtcgacga ggcgactgac ttcatggtcg
     3301 agcagaccgg cttcgagcga ccgcacgcca acgccgaggt caagtggtac acgtaccggc
     3361 cgacctaccc gctgtcctac ctcctcggcc gccagctgct gctgcgtctg cgcgccgatg
     3421 agcaaaagcg cctcggctcg tctttctcgt tgcgcgggtt ccacgacgcg ctgctcaacc
     3481 agggcgggct gccgatcagc ttccagcggc ggctgctggc ccaggacgcc tgaccgtgcg
     3541 tctcgtcccg agcatcgacc tggccggtgg acgctcgcgg ctcgtgttct ggccgggggc
     3601 ctcgagtggc accggcgcac cgactgaccg gccggagcgg atcgctgccg cctttgtggc
     3661 catgggcgca ccgatgatcc acctcgtgga cctcgacggc gcccgtcagg gagtgcccgt
     3721 gaacacggcc gcgatcgccg cagtggcgcg cgacgtcgct gtgccgctgc agctggctgg
     3781 tggagtggac gggccgcgcc agatcgagct tgccttcgct gcaggggcga cgcgcgtcgt
     3841 cgtgcccgtc tggatcgccg ccgagtcgct cgatggcttg cgtgagtgtc tgcgcgtcgc
     3901 cggcgactgg ctgtcagtcg gcctcgatgc gcgcgacgag cagatggcag cctatccatg
     3961 gcccggcgcc gcgccatcgc tggacgagct cgccgctacc ctcgcaggag aaggcgtgcg
     4021 ccgtttggtt gtgtcgcact ggacggccga tgatctgcct cgtctggcgg cgatcaggac
     4081 gcgccatgac atagcggtcc agctggccgg cggcgtcagc gaacctgccc agatcgaggc
     4141 cgcggccgcc gccggcatcg acacgctgat cctgggcgag gcacttctct caggagcaat
     4201 cgagtaccca cagaaggtgg ctatgaatgg ctaagcgcaa gcacgtcgca cagcggtcac
     4261 gcatcaacga gggcgcacaa cgacccggct tcacacgccg ctccagcctg cccccgccct
     4321 cggagcgacg gtcgtcagcg tggatgtggc ttgtgctcgg ggcgctcggg atagtgggag
     4381 tgatcgtggt cgccgcctac gccggagggt tcatcggccg gcccggtgcc acgccgtcgc
     4441 cgtcgcccgc tccacggccc agcttcgcca acctggcgcc gccctccgcg acaccactcg
     4501 ctaacccacc gtccgcgccc gccggcgacg gcaccacggc gaccatagtc acggacctgg
     4561 gcacgatcgt gatcgatctc tataccgact cagcgccggt cgcagcacag aacttcatca
     4621 acctggcaga ggctggcttc tacaacgggg tcggattcca caggatcatt cccgacttca
     4681 tgatccaggg cggcgacccg cagggcaccg gcggcggcgg cccgggctac gagatcaggg
     4741 acgatcctgt tgtcggcaac tacagccgtg gccaggttgc aatggcgcgg ccggccaacc
     4801 ctgacgggag caagaaaccc gattcggccg ggtcgcagtt cttcatcatg gtggcagata
     4861 cgcccgggct ggccagtggc ggctacgcaa tattcggcac cgtggtttca gggatggata
     4921 tcgtcgacca gatcgtcctg ggagacacca cggggccgcg cggggacctg gcagccaacc
     4981 cggtgatcat gcggagcgtc acgattcagc gtccctgagt gagactatcc acaccaagtt
     5041 gcggaatatt cattattcag cctgcataac cccttgagcc accggagggg gtcaggtaaa
     5101 ctggctccga tgtcgcgttt aggcgtggca taagctgccc caggcgcaag gtcatgggta
     5161 ggttgggtta gggtagccgg tgcagccggc gtgatagatg cccttgtggc gtggcaggag
     5221 cccagcaact tcggttgctg gatcggccgg gacagttgac ggacccctct tcggaggggt
     5281 tcgtcttttt ttggcgtaac ctcaccaggc gagcgcccgt agctcagcgg atagagcgtc
     5341 tgcctccgga gcagaaggcc gtgggttcga gtcccgccgg gcgcgccagt catttgccca
     5401 gccgcgcgat cagccgcgca gggtcccttg tcgaagcacg gcgaggaggc cggtcgcgcc
     5461 gaggggcttg ctgaagaaga agccctggcc gtagcggcag ccgagctcgg ccaggcgcca
     5521 tgcctgctcg cgcgactcga cgcctttggc gaccacttca acaccgcgca cgtcggccag
     5581 gttgatcagc gatagcgcga aggcctgctc ctgagccgga ccgtcgatcg cggccacgaa
     5641 ctcgcgcgcc agcttgacct ggtcgacctg caccgccttg agatcggcga tcgaggtcgc
     5701 gacgccaaag tcgtcgagca cgatgcgcac ccccgcgtcg cgcagctcgt tgagtgcggc
     5761 gccaacggcc ggcatatcgc cggatacggc gccggtgagc tccaccatga gctggcgcgg
     5821 ctcgaggccg gcgcctgcga tcagggcgag cgtccgcgcc gcggcgtcgc gtgcgcgcag
     5881 ctgactgggc gcgacgttga cactgacgac gcccgggcga tcgtttgaac gcggccacgt
     5941 cgccgcggcg cgcgccgcct ggtcgagcac gaacgtgccg atgccgtcga gcagccccgc
     6001 ttcctcggct acggccacga actcacccgg cgccacggtg ccgcggcgcg ggtggcgcca
     6061 gcgcaccagc gcttcggcgc cgacgaccgt gccgccgccc agccggacga caggctgaaa
     6121 ctcgagccgg aactcgccgc gctcgattgc accaaccaga tcgtggcgca ggtcatcgac
     6181 gccgtggggc tgcttggcca tgaccaaacc ataactcgcg accattacgg ggggtttgta
     6241 cgatgccgac acactgttcg atataggaga tttggttgcc ggtcctatcg gagcgtgccg
     6301 agctgccctg gtggtcaaag agccgcgtac agcgcaagcg ctcgctcaac accgaccgga
     6361 tcgtcgaggc cggcatggcc gtgctcgatc gcgacggcct cgacggacta tccgtgcgcc
     6421 gccttggtgc ggagctcggc tcgggcgcaa catcggtcta ttggcatgtg cccggcaagg
     6481 aggcgctgct cgacctcctc gttgatcgac taatgggtga ggcagctgac gagacgcgcc
     6541 atgacccggg cgccacgtgg cgggcggagc tggccgccca ttcactcgcg ctcagagatg
     6601 tcctccagcg tcatatcggt ggcgcctcac tgctcgcgtc gcggatgccg gttggaccta
     6661 acgggctgcg cttcatggaa ggtgtgctga gcgccctggc ggatgccggg ttcggcgggc
     6721 accaacgggc gctggcatac gcggcgctga gcggctacac gatcggccag gtcgtcctgg
     6781 agtcacgccg aggaccgacc actcctaacg accaaccagc cgggggcgag cagctgcaac
     6841 gcctgggcgg actgctccag ggcgctccgc gcgaccgcta ccccaacgtc gtagagagcg
     6901 cggccgacct ggccgagctc accgacgagg aggcattcag ttacgggctc cagcggatgc
     6961 tcgatggtct cgaggcggag ctcggccctg tcaccccgaa ccggggcgcg atctgatcac
     7021 ttcgcgcgcg gccgcgaaga cgtcatcgag catgcccggt gtcagacgcc cggtgaaggt
     7081 gttctgctgg ctcggatgga aggagccgat caacaggtag cggccgacct cggcccgcgc
     7141 gccgtggcca aagcgcgttc gcgaggccgt cttgttgccg ttggccgata gcgcccgcag
     7201 cgtgccgtcc cacgcgaagg cgcccaggca gacgatcagt cggagtcgcg cgagcgcagc
     7261 gatctgtccg gcgaggtagg gcaggcagtt gtcgcgctcg gtcggcgttg gcttgttggc
     7321 cggcggcgca cagcgattga ccgcggcgat atacgcgtcg gatagccgca ggccatcatc
     7381 gcgcgccact gacgttggct ggttggcgaa cccggcgcgg tgcagcgccg cgaacaggaa
     7441 gtcgcccgag cgatcgcccg tgaagatgcg ccccgtccgg ttgccgccgt gcgccgcggg
     7501 cgccagcccc acgatcagga gtcgcgcggc gggatcgccg aaaccgggta gcggccgcgc
     7561 ccagtaggcc tggccggcga accgggccgg cgggttgcgc gcggcctcct cacgccacgc
     7621 gaccaatctc ggacagcgcc ggcattcgac gatcagcgaa ttgatctggt cgagggtcac
     7681 cgaacggcgc cggccgtcgc tccactgagc aggctcgtca gctgcgcccg gttctcgacg
     7741 gacctgaagt cgtgcagcgg attcgaggcc agaagcgttt cgaccgccgg ccggccgacc
     7801 tcgcgcacat acagggccgg gtgaatgtcg cccagatcgt cggtttcgac cgcgatcgct
     7861 actgcggccg gcccctcttc gtcgacgaat tgggccagcg ggacgacacc ctcgtcgcga
     7921 atgccggatc ccgagtcgag cgagaacgcg cgggcgaggt acgacttctg gttccacaat
     7981 tccggcgcgc gcgttgcctc gctcagcagc aggtcgacga gcgcgtcagg cttgacctca
     8041 ggccgcggca gctccacccc gaaatgcgtc cgcgccaggt ggtggaccat cgtccgggcg
     8101 ttgtagcgcg ccccgtggac ggcgcccgag ttggccggca gcccgtactt cttcatgccc
     8161 gccacaccct ggccgatcgt gcccgcgaag tagatgcccg gcacggtcgc gctctcgtag
     8221 tagttggtca tggccggcag gccgctgcgg ccaaagacgg cgacgcccag atcgggcagg
     8281 tcgcgcagcg gacacttgaa cccggtcgcg gcgatgacct cgtcggcttc gacgctcagc
     8341 tccgcgccgt tctcggagcg aatgcagtgg acgcggaagc cgccgggcgc gcgctcgatg
     8401 cgttcgatcg aggcgttcag gatgaacacg ccgccaccca ggttctggtc ctcgaacggc
     8461 agcacgtagc gcgcccgcac gccgagcaac gagtgggtgt tgacgctcag cttggccggc
     8521 cgcggtgacg ccaggacaat gcgctgcgcc cactggagca gcccggtggc gagctcgaaa
     8581 ccggagttct ccttgccgac gatgaacagc cgtttgccgg cataggtcgc cgccgcgcgg
     8641 gtgtcgacgt agtgcgagac gagctcgaag cccggtgtgt cgggcaggta gggctcggca
     8701 attcccacgg caaagatcgc cgcctgacag cggtactcgc catcgggtgt cgagagcacg
     8761 aagcgctcgc cgtcgcgccg cgtggcctcc cagcgacagt catagcgcac ggccaagccg
     8821 gcctgctcga cgaacgcggc gaggttggcc tccatctccg gccgcgacgg gaacgacgac
     8881 gtgccgtcca tcagcgtcgg catgatcgcc cgcgcccctg gctcgtcggc cagcagcgag
     8941 ttccagtcgt accactgata ccagcggtcg tcgcgcggca cgggcgcgta gggcttggtc
     9001 cacgacagca ggcgctggaa gaatgggaag tggcggaaca tgccaccagg ggatggatcg
     9061 gccgagatca ccgcgtgctc gatcccgagg cgcttcaaga agtacgaggt ctggaggccg
     9121 cccggcccgc taccgacgac cactaccggg tagtcgcccg gggggaacgg gcgctggtct
     9181 agctccgcca tcgactggca ttgcgtagac gcagggcaac cgccttgagc atccgataca
     9241 tgacctgggg gtgatcgcgc aggaagcctt ccaggtccgg gccgcccagg tgcaggacgt
     9301 gcagctggcg cgtggtgacg atgtcggcca cgggcggctc gccgagcagg atcgacatct
     9361 cgccgaagaa gtcgcccttg gccagccggg cccgctcctc gccgtcgatc ttgaccgcga
     9421 cctcgccatc gaggatcacg tagaaacccg tgccggtgaa gccctggcgc aggatgcgct
     9481 ggccgaccgg gaatgattcc tcggtcatct tgtgggcgac ctgctccagc tgcggccgcg
     9541 tcaggtcggc gaagagcgtc aggctcgaga gcatgtcgac cagctcctgg gcggtcatcg
     9601 ccatctagtg gcccagcttc tcgcccgcct ggcgcacgcg catgacgaga cccttgatca
     9661 tgttcaggct cagctgcgga tcgctctcga ggagcgccag caggtcccac gaagcgagtg
     9721 ccagcacgct cgtcgactcg gcggcttcga cgctcgcgtt gcgcggctgc tggtcgatga
     9781 ccgacagctc accaaagaat tcgcccttgc cgagccgagc aatcacgtct gagccgcgca
     9841 gcaccttgac cgagccactc aggaccacgt acaggccggt cccgacctgg ccctggcgaa
     9901 cgatgaactg gccggccgcg aattcctgct cgccggcaac tgcggcgagg cgctccatcg
     9961 attcggcgct gatacccgcg aatagcggca cgccggcaag cgcggccgcc ttgtcctggg
    10021 tgctgagtgc catcggccgc acgatatcag tcgccgccat ggtcaccaga gcggatggaa
    10081 aagccgcgca ggtcaccgac tgatggcaga cggttcgcat ccacccgctc cacgtgcgcg
    10141 ccgggcgggc ctgcatgaag gtggcgcgtc agctcgtcga gcgcggccgg gctgccctcg
    10201 gccacgacat ggacggtgcc atcgtgctcg ttggccgtcc agcccgccag gccgagctct
    10261 ttggcgcggc gcacgacgaa gtagcggaag ccgactccct ggacgtagcc gcgcactgta
    10321 gcttcgagcc tttcggcccc gagccgctcg gtcacgccgc gcggcgggcc gtgggccacg
    10381 cgatcagcag cgcgtcaacc gcgagctcgg gattggcgta agcgtcgagc gcgcggctga
    10441 ctgcctcgag acgatccagg aactgcgcgg cctgcttcgc acccacgctc ttgccggcga
    10501 ccgccagctc atcgagcagc tcgtgctggc gcagctcgcg cgtgccgcca cgggcggcca
    10561 ctgccaggtc gcgcgccact tcacgccaca cggcgaggag ctgggccgcg gccgcccggc
    10621 gctcagcggg gctgatcttt gcggcggagc tccccgagcg gcgcgccggt accggtgtct
    10681 cgctttcgtc gactcggccg gtggtcgcgc gcagcagctc cgcggcatcc gcgagcagct
    10741 ccggtggcgc tgccaggcga cgccgtctgt ccgcatgcag gagatcgagc agcgcagcgg
    10801 ccaggcgcga ctggaccact gttgcctcgg gttgcgcggc cagggcaatg gccacgcccg
    10861 ggcgcccact ggcaagccgg gttaaggccg ccgcccgggc agcgtcggcc aggccgcgct
    10921 ccgccagcag cgcggtgatc ggctctgtcg ccaccgggcc aaggcgtgcc cgggcgcagc
    10981 ggctgacgac ggtcggcagc aggccggccg agtcatcggc ggcaaggatc agtgccactc
    11041 gcgcgggcgg ctcctcgagg gtcttcagca gcgcgttctg ggcatcgatg ttcatgcgct
    11101 gcgcgctctc aatgatcgcc acgcggaagc gaccctccag cggcagcagg gcgagcgcgg
    11161 aggcgaggcc ctgcacctgt gcgacgcgaa tctgctgtcc ggcaccctcc ggcgccaaca
    11221 gatgcaggtc ggggtgattg ccgtgggcca ccttgtggca cgcggcgcat tgcccgcacg
    11281 gccgatcggc cgggtcagcc gcaagacaca gcagccccgc ggccagatcc atggcgagc
//
LOCUS       DATBRX010000003         4761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1789277, whole genome shotgun sequence.
ACCESSION   DATBRX010000003 DATBRX010000000
VERSION     DATBRX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789277"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /gene="priA"
                     /locus_tag="VIF08_00105"
     CDS             complement(<1..1713)
                     /gene="priA"
                     /locus_tag="VIF08_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY8134412.1"
                     /translation="MANTFVEVAVDAAGPAGGQTFTYHLPDALADVAVGEAVLVEYGR
                     RRALGIVMSRGEEAPARETKAVEARVRSDGPLLPPLQVALAQHITQHYLAPPALVIRQ
                     MLAPGALERVERVIRQTNDGLEAEWSVVAARTRDKVERRVSLTDAGRSGLEVPGLGPR
                     QRAVLAELMASDDAISAARLAERHGSSAIPGLLKRGLVALEVARVERLPLADRAAGER
                     GTRPLDTELTPPQRTAVDLVRDHITAGRHATLLLEGTTASGKSAVYGAAIAAARDQGK
                     GALVLVPEIALAVPLLDRLRHDLGEDVALLHSALADGERADEWKRIRDGEVKVVVGTR
                     IAALAPLGDPGVIVVDEEHDSAYKSDRTPRYQARDLAVELGRLANAPVILGSATPDVA
                     TLGRARGGEYEHVTLPRVAGSRATVEVVDLRAELAQGNKGLLSGALTSALERLDTKDG
                     EQAILLINRRGSASVVLCRDCGYVQVCPECHRPLVLHAQSLSLRCHHCGATAPLARRC
                     PACDSPRIRYLGGGTERVERELGIRFPNLRVTRLDRDVVERKGAATRVIDSFASGQVD
                     VLVGT"
     gene            complement(1722..2339)
                     /locus_tag="VIF08_00110"
     CDS             complement(1722..2339)
                     /locus_tag="VIF08_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8134413.1"
                     /translation="MKPNRSILISGPSRTGKTTTAELLAARYGIDKVKIGERFRELTG
                     IDTSKFVDRPEELDRQIDEMQSKLIREATAQAPFILEARLAAFLASKERQLQPDLPIV
                     TVLFWAPEHVRMERQLRKVRRDDPNTRMTLEDLRDKEREREAGDLKLWRAVHPDLDDK
                     NVFDPALTDDRGKPVYDFVVDTRLGMPDTCADAVHRYLLKVGAFG"
     gene            complement(2336..3265)
                     /locus_tag="VIF08_00115"
     CDS             complement(2336..3265)
                     /locus_tag="VIF08_00115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8134414.1"
                     /translation="MGERLTVSVPAGAGRRADRVVADAVGLSRSYVQRLIEEGRVTEA
                     GKPVKSKDVLPAGAALELDMPDRPTPTIEAEDIPLAVVYEDADVLVINKPAGLVTHPS
                     PGRTGGTLVNALLNRAPASEYGAVAGADRPGIVHRLDRDTSGLIVVARNDHAQAALMA
                     QLKARRVKKTYLALVEGSPAAEVGRIEAPIGRDPRHYSRMAVLPNGRPSVTGYRVRER
                     LHGWTLLEVDLLTGRTHQIRIHVASIGHPVAGDPVYATGAARRGPEGLTRLFLHSWRI
                     EFASVSSEKLIRAEAPLPPELESVLVGLRINPR"
     gene            complement(3265..3804)
                     /gene="lspA"
                     /locus_tag="VIF08_00120"
     CDS             complement(3265..3804)
                     /gene="lspA"
                     /locus_tag="VIF08_00120"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY8134415.1"
                     /translation="MNLSTSRGRWILFATVAIVVIIADQLSKLWIDANFAVASVHALP
                     GQPQPTQVLGEFVRIAKNYNSGGIFGLFGNTAAILALTSTVVIGLIVVYQWREGTRSP
                     WPLSLALGLLFGGAIGNFIDRVRLGAVIDFVDMGIGAWRFYTFNVADSAISTSLLLLI
                     LMGLFGERIAARMRDRPAN"
     gene            complement(3801..>4761)
                     /locus_tag="VIF08_00125"
     CDS             complement(3801..>4761)
                     /locus_tag="VIF08_00125"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEY8134416.1"
                     /translation="SRSRMRSTASAAEREAAFATLHLALTTASRVLAPILPFMSEAMY
                     QNLVVTALPSQPDSVHLTAWADKLLQPHSDEALEESMATLRRAVELTRTLRGQAGLRL
                     RQPLREMWLAIPGGRLATDSPGDEAELLELLADETNVKRVTVIGDESELVERRVKPLL
                     PKIGKRLGAQIPAVMAAARSNDVEYLPSGGVKLAGVELAPDEVEILATPRPGTAVAHD
                     EGLVVVIDTEIDDALRAEGDARELIRAIQDLRKEAGLELDDTIDLWFGGLGSPVDLKR
                     IAEETLAIVNIGSPQAEGATVGQVELQIGGPVVIGIRRRSSAV"
BASE COUNT          703 a         1670 c         1646 g          742 t
ORIGIN      
        1 ggtgccgacc agcacgtcga cctggccgct ggcgaaggag tcgatgaccc gggtcgctgc
       61 gcccttgcgc tcgacgacat cgcggtcgag gcgggtcacg cgcaggttgg gaaaccggat
      121 gccgagctcc cgttcgacgc gctccgtgcc gccgcccagg tagcggatgc gcggcgaatc
      181 gcaggccgga cagcgccggg ccaacggtgc cgtggcgcca cagtgatggc agcgcaaaga
      241 aagtgactgg gcgtgcagca ccagcggccg gtggcactcg gggcacacct ggacgtaccc
      301 gcagtcgcga cacaggacga cgctggcgct gccgcgacgg ttgatgagca ggatcgcctg
      361 ctccccgtct ttcgtgtcca gccgctcgag tgccgaggtg agggcgcccg acagaaggcc
      421 cttgttgccc tgcgccagct cggccctgag gtccacgacc tccacggtcg cgcggctgcc
      481 ggcgacgcgc ggcagggtga cgtgctcgta ctcgccgcca cgcgcccgcc cgagcgtcgc
      541 gacgtctggc gtggcgctgc ccaggatgac cggcgcattc gcgaggcggc ccagctccac
      601 cgccaggtcg cgtgcctggt agcgcggcgt gcgatcagac ttgtaggctg aatcgtgctc
      661 ctcgtcgacg actattaccc cggggtcgcc gagcggcgcc agggccgcga tgcgcgtgcc
      721 gacgacgacc ttcacctcgc cgtcgcggat gcgcttccat tcgtcggcgc gctcgccgtc
      781 ggcgagggcg ctatgcagca gggcgacgtc ctcgcccagg tcgtggcgca gccgatcgag
      841 cagcggcacg gcgagcgcga tctccggcac cagcacgagc gcgcccttgc cctggtcacg
      901 tgctgcggcg attgccgcgc catagaccgc gctcttgccg ctggcggtcg tgccctcgag
      961 cagcagcgtc gcgtgccggc cggcggtgat gtggtcgcgc acgaggtcga ccgccgtgcg
     1021 ctgtggcggc gtcagctctg tgtcgagcgg tcgcgtgccg cgctcccctg cggcgcggtc
     1081 ggcgagcggc agccgttcga cgcgtgccac ctcgagcgcg acgaggccgc gcttcaagag
     1141 gcccgggatt gcgctgctgc cgtggcgctc agctaatcgc gcggcactga tcgcgtcatc
     1201 cgaggccatc agctcggcga gcaccgcgcg ctggcgcggc cccaggcccg gcacctcgag
     1261 cccagagcgt ccggcgtcgg tcagcgagac acggcgctcg accttgtcgc gcgtccgcgc
     1321 ggccacgacc gaccactcgg cctcgagccc atcgttggtc tggcgaatga cgcgctcgac
     1381 tcgctcgagc gcgcccggcg cgagcatctg gcggatgacc agcgccggcg gcgcgaggta
     1441 gtgctgcgtg atgtgttggg cgagggcaac ctggagcggc ggcagcagcg gcccgtccga
     1501 ccggacgcgc gcctccaccg ccttcgtctc acgcgccggc gcctcttcgc cgcgcgacat
     1561 gacgatgccc agcgcccggc gccggccgta ctcgaccagc accgcctcgc cgaccgcaac
     1621 gtcggcgagc gcgtcaggca ggtgataggt gaaggtctgg ccgcccgctg gcccggcggc
     1681 atcgaccgcg acttcgacga atgtatttgc caacgatccc tctacccgaa cgctccgacc
     1741 ttcagcaggt aacgatgcac tgcatcggcg caggtgtcgg gcatgcccaa gcgcgtgtcg
     1801 accacgaagt cgtagaccgg cttgccccga tcgtcggtca gggccgggtc gaacacgttc
     1861 ttgtcgtcga ggtcgggatg gaccgcgcgc catagcttga gatcgcctgc ctcgcgctcg
     1921 cgctccttgt cgcgcaggtc ctccagggtc atccgggtgt tcgggtcgtc gcgccgaacc
     1981 ttgcgcagct ggcgctccat gcgcacgtgc tcgggtgccc agaagagcac cgtgacgatg
     2041 ggcagatcgg gctgcagctg gcgctccttg ctcgccagga acgccgccag cctggcctcc
     2101 aggatgaacg gcgcctgagc ggtcgcctca cggatcagct tcgactgcat ctcgtcgatc
     2161 tggcgatcga gctcttcggg tcgatcaacg aactttgagg tgtcgatgcc ggtcagctcg
     2221 cggaacctct cgccgatctt gaccttgtcg atgccgtagc gggcggccag cagctcagcc
     2281 gtggtcgtct taccggtgcg cgacgggccg ctgatcagga tcgagcgatt gggcttcatc
     2341 gcgggttgat ccgcaggccc accaggacgc tctccaattc cggggggagc ggcgcctcgg
     2401 cgcggatgag cttctcgctc gacacggacg cgaactcgat gcgccacgag tgcaggaaga
     2461 gccgcgtcag tccttccggc ccgcgccgcg cggcgccggt cgcgtatacc gggtcgccgg
     2521 caaccgggtg gccgatcgag gccacgtgga tgcgaatctg gtgcgtccgg ccggtcagca
     2581 ggtcgacctc gagcagcgtc cagccgtgca gccgctcacg gacgcggtag ccggtcaccg
     2641 acggccgccc gttgggcagc acggccatcc ggctgtagtg gcgcgggtcg cggccgatcg
     2701 gtgcctcgat gcgcccgacc tcagcggccg gcgagccctc gacgagcgcg aggtaagtct
     2761 tcttgacgcg gcgtgccttg agctgcgcca tcagcgcggc ctgggcgtga tcgttgcgcg
     2821 cgacgacgat gagcccgctc gtgtcgcgat cgagacgatg gacgatgccc ggtcgatccg
     2881 cgcccgccac cgcaccgtat tcgcttgccg gggcgcgatt gagcagggcg ttcacaagtg
     2941 tgccgcccgt tcgaccgggc gacggatgcg tgacgagccc ggcgggcttg tttatcacca
     3001 gcacatctgc atcttcgtag accaccgcaa gggggatgtc ctcggcttcg atcgttggtg
     3061 tcggccggtc aggcatgtcg agctccagcg cggcaccagc gggcagcacg tccttgctct
     3121 tgaccggctt cccggcctcc gtgactcggc cttcctctat cagccgctgg acgtacgagc
     3181 gcgacaggcc cacggcgtcg gcaacgacac ggtcggcgcg acggccggcg ccggccggga
     3241 cgctgaccgt cagccgctca cccatcaatt ggccggcctg tcgcgcatgc gcgccgcgat
     3301 ccgctcgccg aacagtccca tcaggatcag cagcaggagc gaggtgctga tggccgagtc
     3361 agcgacgttg aacgtgtaga agcgccatgc gccgatgccc atgtcgacga agtcgatcac
     3421 cgcgcccaag cgaacacgat cgatgaagtt gccgatcgcg ccgccgaaca gcagccccag
     3481 ggccagcgac agcggccacg ggctgcgcgt cccctcgcgc cactggtaga cgacgatcag
     3541 gccgatcacc acggtcgagg tcagggccag gatcgccgcc gtgttgccga acaggccgaa
     3601 gatgcctccg ctgttgtagt tcttcgcgat ccggacgaac tcgcccagga cctgcgtggg
     3661 ttgcggctgg ccgggcaacg cgtgcactga ggcgacggcg aagttggcat cgatccagag
     3721 cttcgatagc tgatcggcga tgatgacgac gatcgcgact gtggcaaaga ggatccagcg
     3781 accccggctt gtcgacaggt tcaaaccgcc gagctccggc gccggatgcc gatcacgacc
     3841 ggtcccccga tctgcagctc tacttggcca acagttgcgc cttcagcctg cggcgaaccg
     3901 atgttcacga tcgcgagcgt ctcttcggcg atgcgcttca ggtcgaccgg cgagccaagg
     3961 ccaccgaacc acaggtcgat agtgtcatcg agctccaagc cggcctcctt gcgcaggtcc
     4021 tggatggcgc ggatgagctc gcgtgcgtcg ccctcggcac gcagcgcgtc atcgatctcc
     4081 gtgtcgatga cgacgaccag gccttcgtcg tgcgcgacag ccgtgccggg acgcggcgtg
     4141 gccaggatct cgacctcgtc gggcgcgagc tcgacgccgg ccagcttcac accgcccgac
     4201 gggaggtact ccacgtcgtt gctccgcgcg gcggccatca ccgctgggat ctgtgcgccg
     4261 aggcgcttac cgatcttggg caggagcggc ttgacgcggc gctcgaccag ctccgactcg
     4321 tcgccgataa cggtcaccct cttgacgttc gtctcgtcgg ccaagagctc gaggagctcg
     4381 gcctcgtccc ccgggctgtc cgtggccagc cgtccaccag ggatcgcgag ccacatctcg
     4441 cgcaacggct ggcgcaggcg cagcccggcc tggccgcgca acgtgcgcgt cagctcgact
     4501 gcgcggcgga gcgttgccat tgactcctca agagcttcgt cggagtgcgg ctgcagcaac
     4561 ttgtcggccc atgcggtcaa gtgcacgctg tctggttgcg acggcagcgc ggtcacgaca
     4621 aggttctggt acatcgcctc cgacatgaag ggcaggatgg gcgcgagcac gcggctggcc
     4681 gttgtgagcg ccaggtgcag cgtcgcgaac gccgcttccc gctccgcggc gctcgccgtc
     4741 gaacgcatgc gcgagcgcga a
//
LOCUS       DATBRX010000004        23070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3353513, whole genome shotgun sequence.
ACCESSION   DATBRX010000004 DATBRX010000000
VERSION     DATBRX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 23070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23070
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3353513"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            614..2428
                     /locus_tag="VIF08_00130"
     CDS             614..2428
                     /locus_tag="VIF08_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8134417.1"
                     /translation="MTEVRGAPARHAAADLRRLRWIGFALPILGLVGIEAFRFAFVED
                     VPLQQAEHVAIGAIAAVGILAFAVVMFRLIDRAESQVLRQNRELTAINAVSTAVQGEL
                     GVEQIIDAALDVVTERTGATEASVMVFSRQPGRSPTLERRLVRSPHAELSSMAEQMPH
                     LVEIPLAHGSSIVGRMRLHLAADALQPDVLTSATLNNVGHQLACAIEIGQLIGSLQRR
                     ESEDRGLYSVLLRISSQKPLPETLLALAQQARELLDADAVQVSLSAASMLMIEPPAGR
                     VRPRDGAASIVQRDDGGLTVSAVRPGDGAKHASGVEPVRLSLTNSEMAFGELSVVPAA
                     GTHYSEREWGFIHRIAELAVIAISAARMREQERQVAILAERERIAREMHDSLAQVLGF
                     IHLRLVALKAKVAPIGSPHMNDNLDELTGVAHDAYRDVREAILGLRESSRNGRSLFES
                     LRAYLERYQHQAAIETSFETDFDQPATLTPAAEIHVIRVIQEALTNTRKHARARHVTV
                     RATTRDGFVAFVVEDDGRGFNLADVALSHEGGFGLQAMRERMELIGGSLSIDSAPRHG
                     TRITALVPAVANGATVSDGATVSDGTTAREEVARVTSN"
     gene            2412..3236
                     /locus_tag="VIF08_00135"
     CDS             2412..3236
                     /locus_tag="VIF08_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8134418.1"
                     /translation="MSPATEDLPVPARVLLVDDQPLFRRAIATLIEEQPDFTVIAEAS
                     NGQEALDRARALRPDLVVMDVEMPVLNGVDAVRLLRDEMADTKVVMLTVSEDEDYLFD
                     AVRFGANGYLLKDLQPEQLYEMLRSVLRGESPISPVMAGRLLAEFRRSHADGHASDQP
                     ANARAGFPADGQRSHLPPRPVAPQAPPEELAAADLARFPLASAVAQVLTRRELETLQY
                     VADGLSNKEIGQRLSITEGTVKNHVHNALEKLQLENRIQAAAYIVRQGIAHPPSSN"
     gene            3339..3683
                     /locus_tag="VIF08_00140"
     CDS             3339..3683
                     /locus_tag="VIF08_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134419.1"
                     /translation="MASDSSPKARVLVLYDHALLGEGLASLLSAEPGVGAVAVNGRDS
                     AARAAALGSHPDLVIVEETEQAQPYEPSGSAPVVFVRVDASGECPLGKPLGDPEAIVA
                     LARGLVEARAGT"
     gene            3849..4826
                     /locus_tag="VIF08_00145"
     CDS             3849..4826
                     /locus_tag="VIF08_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8134420.1"
                     /translation="MSNTKTIRLERPGAPTDKTLNRLIGVLIAIIAIGLPAIGVIYYL
                     DRHVDAGPSITGRAVITAEEAVRQYPNQLETRVVLAQAYAADDRPADAIAQYTIILGA
                     EPVNTTALLGRGDLYRKTGQLGAAASDYQALIDVARDTEMANIDRALQSAYYGLGLIA
                     MTQGRPDDAATQLANSLRIDRTDADALDLMGQALIAIGDYETAVEALRDAVAFVPTGW
                     VDPYTHLAQAYEGLADQGGVAYANGMVALNDGRLDDADAALQPLVGGSHSRDALIGLG
                     LTAEQRGDAAAAADFYRRVLAVVPDDFAALSGLSRVGVPATAPLSGSTP"
     gene            4823..5881
                     /locus_tag="VIF08_00150"
     CDS             4823..5881
                     /locus_tag="VIF08_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134421.1"
                     /translation="MTDIALTPDLDQSLPADAGTAPPDALGPEDRRRRRRRLAILAVL
                     SVLAVVLLVFSGWYLLFRKPISLLPIPGFDLPPMPGYSYSLYGMGQPTGIAVTPDGSR
                     IYVTQTSGEDAVLMLDGQGQVMAALQSPDTTTDHVFVYIAINPLTAEVYVSDRPAADV
                     LVFAADGTFLRTFEPPASLVGWQPLGLNFTPDGHLLVTDLGANAVYEFDTAGQLLRTI
                     GSNGMFNYPNAAAEDALGRLYVSDSNNGRLVVFGRTGELLGVIRRGPAAGDLGMPRGL
                     EIDDQGRLYVVDNTDHSIKVYRTSADPVALPEFLGSFGEPGIGEGMFRFPNAVATDTR
                     GRLYVADWNNDRVQVWTY"
     gene            5956..7251
                     /locus_tag="VIF08_00155"
     CDS             5956..7251
                     /locus_tag="VIF08_00155"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY8134422.1"
                     /translation="MRRLASLLAGGALFLLIGAIPALADGGPHTLTGNSGTAGLTSDS
                     CAGCHRTHTAQAPLLLVEEESALCLTCHGDAGNGATTDVMNGIQYATGTTHSATPDND
                     VILGALRGGGFAKARIDTSNATRLTYTRRSGSAGNYTYPLTQAQAVGVLAAGETVTSR
                     HLNPAASAASTTWGSGALGTNTTGAAITLECTSCHNPHGNGQYRILDPTPGDGDGPFV
                     EATADVVVTDVLEPNRLHNYTVIQKLSTSATFSSSGSITAEGLEAGYLLYADQIDDEY
                     GVSVGDYFHRNLPWNGRTDYTAEAAGTPGAVITNRMNDAPNGKPLTFDAQINAWCSQC
                     HTRYLAGSGTYNTYSGDTVFSYRHTNTSNKPCTTCHVAHGSNAAMTGIAATALDPADE
                     PSASSRLLKIDNRGTCQACHDPTDTVPAGTLLGPTPNVP"
     gene            7339..9447
                     /locus_tag="VIF08_00160"
     CDS             7339..9447
                     /locus_tag="VIF08_00160"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY8134423.1"
                     /translation="MSQEHQRAAALRLSRSLFVLLALATGLALAYLPAQPGVAVAETA
                     DTASPAPGLESSPSPDPAGLDPTPSPTLEPAASPSPDPALEPSPDPSTAPTADPSASP
                     VAAFAVMQHAFARVATAAAPPTYLAPGAPLTDEPRYEVFRISFDLTNADALDRQFTPL
                     LEFSSDGTTFARVPDETTGAEAAFHVAREWVAQPNGGTTIGPSLVIEAAGGVSGALER
                     VRSSGLNPLPGQIVRPGSSLSVEFSVKATAGAAYNATYVFRLTDDGAALPGASAATLT
                     MSARPALQLTPGQRSGVPGDASARGVTRYGLQASSTSVHTPAFDLVSDSCVACHRTHT
                     AAGSLLTAQPTQYSLCASCHDGTNLPDIAAAYAGVPANDATARAYYQHDPTQVSGPGK
                     TNDCAECHNPHNITDAAAAETVSGWTASGMLSGIGEVELEYQLCYRCHASAATLPSNS
                     GQPPSRYALDKALEFDPGNASYHPIESPGTNQTAALANSLAASGTSPYKLWSWQTTST
                     IRCVNCHADARRADAALTPPGSSLAASASLAIHASPERGILIQPYQDRSLNGPIEPYA
                     AADFALCYVCHSEAPFVDQTGQVRDGQNGTVADTNFRYHGVHLSNPDVMSDRGFPGTD
                     IDTPGYGAGFAICAECHFRIHSSAFPADGRTPGSRLVNFAPNVTAPTGGVLSWQPVTG
                     TSAGSCTLKCHGAPHNNVEY"
     gene            9575..10087
                     /locus_tag="VIF08_00165"
     CDS             9575..10087
                     /locus_tag="VIF08_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HEY8134424.1"
                     /translation="MSRLTSGTHRRTSIQALLLVVTLLGTAAIATACGGSHATSPTNQ
                     AEPSMAAMDTAHAAAWAARPGYTQVSQRTEEAYQYAMEHPLIVQWMPCYCGCGGIGHG
                     SNLDCYFEPSAGGTIVFEEHASYCEICVDITLRTKQLANSGATLASIRTAIDAEFGGA
                     GPGTDTERPL"
     gene            10087..11094
                     /locus_tag="VIF08_00170"
     CDS             10087..11094
                     /locus_tag="VIF08_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8134425.1"
                     /translation="MDGSPVSEHELLSRLGLSDGASEDQVETAHSEVVAFLAGAPADL
                     RDWARSQLAAADEAYALLSDRAGSLQSRPRPAAKPVKQSVQPPSRTLPARAIQQGVAE
                     EPRQPFLSRIGRVGRVAITTIALAGVLVGAYVVYASDLPSVPGLSGTPAPGGQQTALD
                     TARVAELMQTIADNPSDIAAFQELGDLYFTARDYQTAAQWEQRVLDIDPNDVTAHLAL
                     GAAQYNLGNSADAESHWRRVIELNPNDTDALVEAHYDLGFMYFSANPPDVDKTIAEWE
                     QVIELAPDSDIAQTVAAHLQTLEQWNASGSPTTSGSPSPTSAATPTPAATAQPTATAG
                     Q"
     gene            11091..11825
                     /locus_tag="VIF08_00175"
     CDS             11091..11825
                     /locus_tag="VIF08_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY8134426.1"
                     /translation="MTELSLLVAFVAGVVSFASPCCLPLVPAYLGYMVGSDPTAAIRR
                     RDALVQSLFFVAGFSVVFISLWASVGLIGYALRDQAWILRQVGGAVLVFMGLHVAGVI
                     NVAALYREIRLPLPARLQPAGMGFGEHPAGAPSHGRSAMLGVVFAAGWTPCIGPILGG
                     IIGLASVSTSVAQGTSLLVAYAAGLGVPFVLMAVGATWVSPRLGFLRTHHTAVSRVTG
                     AMLIVVGFLMITNTFARLSGLIPAAL"
     gene            11839..13311
                     /locus_tag="VIF08_00180"
     CDS             11839..13311
                     /locus_tag="VIF08_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY8134427.1"
                     /translation="MTQLSRTTAIERRAAAASIGSDLDSDAVFSPSGALEWLWATFTS
                     MRLALALMLGLALLALAGSVIIQAPAGVASDPGAYNAWLQGLKPRYGGFTTIFDKLGL
                     FNVFASVWFRGLAVALTTSILACSIKRAPTLWRAAVHPRTRMSETFYGSAPYSENIAT
                     RMSTADALATTQRVFRANRYRTIVDTDATTGITHLYVDQNRWAPFGTVIAHLAVVIVL
                     VGGMVGSIMGYRDEGFAVPVGSTVAVGGGTGLSVEAVSFSDSYYDNGAPSDYASSLVV
                     YREGTPVAAKTIRVNDPLEVDGTAFYQSFFGPAAVMAVSDASGASLYQGGVPLLWSSD
                     DDTRRIGQLALSDQNILVYVVGVASGRVDANIRAGQIQLEIYRTGEQQPIAIETLDQG
                     EPLLIGGLTYTFKREQQFTGLIVARDPGQPLVWLGALALVLGCFLVFLFPNRRIWAAV
                     KAGPDGAQVSLGATARHDAAFAPEFTKLTEQVRLAFAGAA"
     gene            13332..14237
                     /gene="ccsB"
                     /locus_tag="VIF08_00185"
     CDS             13332..14237
                     /gene="ccsB"
                     /locus_tag="VIF08_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY8134428.1"
                     /translation="MFKMSEYSFMAGLISVGLALLCYVAAFASVRLARRQSALALDGG
                     HAGSTMLSGQSVEVTRGPSSLAGYGTLIAWLALIFVSAALVFRTIAVGHGPFANMYEF
                     SIAFAWGVLAAHVYFERRYHLRALGLFALPVALLLLLYATTIPSTSDPLVPALQNNLL
                     LTTHVAVAIVAYGGFAVAFAAAALHLIASYTGSRRLPRLELLDEISYRAVVIGFPFLT
                     LVIVLGAVWANVAWGRYWGWDPKETASLVTWLIYGAYLHARVVRGWRGNRAAWLLVAG
                     FAATLFTYFGNLFFGGLHSYSGLNQ"
     gene            14234..14866
                     /locus_tag="VIF08_00190"
     CDS             14234..14866
                     /locus_tag="VIF08_00190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8134429.1"
                     /translation="MSATLTASAPAPAGAEATTPSSRRRNALATLAILGITGALILAA
                     AYLSNPRLGDGSGNFTPVRPDGQGVGAAPVIDSPAPDFIAALADGSQMRLSDLRGKPV
                     WLTFGASWCQPCRAENPDISAVYAANVGKIAIVQVYMQEDALTVKDYTDRVGITYLTV
                     PDPTQALSAQYRILGIPSHFFIDATGVLRQIKVGSLDPVSMQAAIDGLSR"
     gene            14884..16554
                     /locus_tag="VIF08_00195"
     CDS             14884..16554
                     /locus_tag="VIF08_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEY8134430.1"
                     /translation="MTDAGVPTDQPDDQSDDLSDDLSDELPSAAIGLSDPPRKRPGRL
                     RRVLRRIPFPRPRSRRGAFVLFILVAGFGAALTVGAVATIAYTDSVAFCGTCHTMAPE
                     MKGYEISAHRNVACVECHTEPGLAGWFKAKLNGTRQLIEVIAGTFPTPIPAPDHAMLP
                     SVEVTCKRCHNVGPLLAGGGPIRLVLHDRYALDPPNTRQSVALVVRPNGFGSGRPAVG
                     VHWHVESDVEYLAPDPRAQEIDYVTVRRSDGSFEEFIASGSVTQSDNVGVDVASLARE
                     LTPRRMDCIDCHNRVGHELPTVSAAIDAQISAGQISTDLPNIKQQGVERLSLTYATLE
                     AADQSIAGLRYFYSTNYPLVARGRSAEIEAAIASLQTTYRLVATPEMGVAAGTYPNNI
                     GHQDAPGCFRCHDGAHYKIVAGKVTNETIPSQCSTCHTFPQIGQYESGVLIGQRPSNH
                     DGRLWVFDHKLSVSTADPTGQSCGACHTRTYCENCHATTAINVSHDEMVTNHAAVIRT
                     SGAQACVYCHAPAYCAQCHADPVMPLGPAGTGARPLLPEIAYPVANSP"
     gene            16551..17201
                     /locus_tag="VIF08_00200"
     CDS             16551..17201
                     /locus_tag="VIF08_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8134431.1"
                     /translation="MSAADTAAATEWLTIREASALVGVSVATLRRWCDAGRVHVFTTP
                     GGHRRFDRADVMELLPAVDIPARLDGFADTSLAIVEGYRRATRKFPYLPRRLRELPED
                     ERVQLRQHGRRIVAALVETLDAPSNEEAVHCGQARTAAAACGQIARRSGLSLQDTLAL
                     FVRFRAPFLHELGAVCRRRGLTGAAATALLERASDLMDRMLPAIVSGYEAAEARRP"
     gene            complement(17198..18448)
                     /locus_tag="VIF08_00205"
     CDS             complement(17198..18448)
                     /locus_tag="VIF08_00205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY8134432.1"
                     /translation="MSDRQTRTILHVDLDAFFAAVEQRDDPSLRGKPVIVGGGGPNDR
                     GVVSTASYEARVFGVRSAMPLRTAAALCPEGIFVPVNGRKYSAASKQVMTILERFSPL
                     VEQLSIDEAFLDVTGTETLYGTGEAVARLLKKAVHDETGLTISVGVAANRLVAKIASD
                     LKKPDGLVVVPPGTERDFLAPLAIERLWGVGASTRKALVDYQVRTIGDLAALPQDVLV
                     RRFGKHGGDLASRAQGVGETAVGGHDAAKSVSQETTFDTDVGEWEKLEQTLLALSEGV
                     ARRLRDDHIMCWTVGVKIRDRDFVTITRQKTLDEPTDSTDLIWRTAVALTRKEVKGKV
                     MRLLGVAASGLTEEHQLALFGGGDERRRKAQAAADEVRHKYGSRSIKRARLIDARVRE
                     GFERDPRRLPLVERDRAGKTRDGA"
     gene            complement(18504..18950)
                     /gene="rplI"
                     /locus_tag="VIF08_00210"
     CDS             complement(18504..18950)
                     /gene="rplI"
                     /locus_tag="VIF08_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY8134433.1"
                     /translation="MKVILTADVDKLGKSGEMKEVAEGYARNFLLPRKMAVSATGGAY
                     RAWQHDIASREDKRKREREEAEIAAQRISSTTLTLGVKVGEGGKLFGSITAKDIADAL
                     ARRGIEIDRHKVDLDEPLKMLGTYKVAIKVYPGLTPEVTVDVEPKG"
     gene            complement(18947..19456)
                     /locus_tag="VIF08_00215"
     CDS             complement(18947..19456)
                     /locus_tag="VIF08_00215"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEY8134434.1"
                     /translation="MARSRRRGGGLLRVLNLLPFLPLAGKAPLYARLLWSLASDPRVP
                     ASRKALLGLAGAYIVSPLDIVPEWIPVLGAVDDVAVMVLAIDVFLEGLPEGLVAEKLV
                     ELGIPASELEDDLARVRRVVPRPLRAMMARVPDALDRVAQRISESGVDQKVRAAISAR
                     MPSSEGNPA"
     gene            19590..20882
                     /locus_tag="VIF08_00220"
     CDS             19590..20882
                     /locus_tag="VIF08_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8134435.1"
                     /translation="MPVLNRPSARATVVALLLMAAIGVGCSSSAPTGKIGGTVSVVAS
                     WSGAEQEAFMAMVRPFEQESGVTVEYKGTRDLNGYLWQSIAQGDPPDVAGLPGPGQMA
                     EFARHGSLKDLATVIDVSGYKADTVPAFIDLGTVDGKLVGVFIKATLKGLIWFNPHDW
                     TVGTPDNWVELVAQANVARRGDSKTWCMGLGSDATSGWPGTDWIEDILLRQSGPDVYD
                     EWVAGRLPWTAPEVKKAFELFGSVVADTDGGAAGIIATNFGDAGNGLFTDPPGCLLHH
                     QATFMTSFFQTRAGARDGEYDFFPFPTIDGRFANNVTGAGDLFGMFRDTPQSRALMSY
                     LMTPDAQSIWVARGGALSVNVHVNNYPDEISSRAAATLANATRFRYDASDLMPEQVNE
                     AFLQGVIDYVRDPAALDAILAHIDEVQRSSINTEPVRP"
     gene            20899..22137
                     /locus_tag="VIF08_00225"
     CDS             20899..22137
                     /locus_tag="VIF08_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134436.1"
                     /translation="MKIAAFARPRQMSDINWPRLLATALLAASVISVLASIVLWAGQR
                     FQPPPVSFIDSQLVFVAEMFYAVAYAAMGWLLATRLPGNLLGWVFLVLGLGMAAQLLV
                     TFQVQEGHEAFRQLSYPLLVAAWISSSAHLPMLAVLTNVVFLRFPTGRPLTPRWRGAG
                     WLAFFGAVLVILGMGLQPSGLTWYPSLTNVFAAPFAWRPLLGPITLLGVSMLIVGVTI
                     GTASIVVRYHRSNDRERAQLRWIAVSVVLIALAGIPFVIVRYGLQADYATGHALLATA
                     LVAGCLLPLAAAVAILHHRLYDIDLILNRALVYIPLTGILGGLYAAGVALFQRLFVTV
                     TGDRSDAAVVITTLVLAGMFTPIKNSLQSFVDQRFKPAGATPAVHHDIHTVEQRLAQL
                     EHRLEQLEGPESSARDVTPD"
     gene            22295..22669
                     /locus_tag="VIF08_00230"
     CDS             22295..22669
                     /locus_tag="VIF08_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134437.1"
                     /translation="MATDVVKRTPSWRILMWVGVVGSVLAWAWVWYLGRGPTVVMLLF
                     ALAAVAFSYRANAGSRWALVGLMVTGFAMFLASLYWLAALYTSGGPITVADALTASFL
                     PLVAATFLLAGSATGFRHTRAA"
     gene            22765..>23070
                     /locus_tag="VIF08_00235"
     CDS             22765..>23070
                     /locus_tag="VIF08_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8134438.1"
                     /translation="MTKHDAARKQRTIEYIASTTRQRGYPPSVREIAQAVDLASTSAV
                     HHHLQALEREGMLERGASHSRALRLTPSASIRAGSSGPLPAQVTSLDSHALPVIGEIA
                     "
BASE COUNT         3586 a         8375 c         7440 g         3669 t
ORIGIN      
        1 catgggcaag gccgcgggtc gcggcggcgg catcctcgtg gcagccgcga cagtccgacg
       61 tcgcgtagac ctgtgggcgg agctgccacg agcgggcatt gacgggggtg cgcggcgcgc
      121 cgaactggtc gtagaagccg gtcgccgcga acgcgcccgc gccgaggacg gtggccacga
      181 tcatcatcgc cgcgagaagg agggtcgggc ggcgcagccg aagcagcgtc gaccgtgtcg
      241 cggtaacggc ctgacgcagg gagcggcgcg ccttgcgggt cgccgcgcga cgcctcgtcg
      301 cgagtgcccg cccacggccg cgcgcggcgg agctcacgcc gtccaccggg acgggagctc
      361 actcaccgca gctgaggcgc agcgcatcga tcggtcgctc cgcccgcctt acccggcagg
      421 cagggccctt ccagccaaga gcctatggcg acgtggggca cggcccgcag cgcaggccgg
      481 cccctttcgg gggtccgttg gtcccgtgaa acggtacgtg cggccctgag gggacgcccg
      541 cgcgcggcag gaacatgcgg ccaggtcctc gaagggatcc gctgcgcacg caccatgcgt
      601 gaggaaggac tgaatgaccg aggttcgggg ggcacccgcc cgccacgctg cggcggatct
      661 gcggcgcctg cgctggatcg gtttcgccct gcccatcctc ggcctcgtgg gcatcgaggc
      721 attccgcttc gccttcgtcg aggacgtgcc gctccaacag gccgaacacg tggccatagg
      781 tgcgatcgca gcggtgggca tcctcgcctt cgcggtggtg atgttccgcc tgatcgatcg
      841 cgccgaatcc caggtactgc gccagaaccg cgagctgacc gccatcaacg ccgtctcgac
      901 cgcggtgcag ggcgagctcg gcgtggagca gatcatcgac gcggccctgg atgtggtcac
      961 cgagcgcact ggcgcgaccg aggcgtcggt gatggttttc agccgtcagc ctggccgatc
     1021 acccacgctc gagcgccggt tggtgcgcag cccgcacgcc gagctatcga gcatggccga
     1081 gcagatgccg catctcgtcg agattcctct cgcccacggc agctcgatcg tgggccggat
     1141 gcgcctccac ctggccgctg acgcactgca gcccgacgtg ctcaccagcg cgacgctcaa
     1201 taacgtcggc caccagctcg cgtgtgccat cgagatcggc cagctgatcg gctcgctcca
     1261 acggcgcgag tcggaggatc gcggtctgta cagcgtcctg ctgcggatct ccagccaaaa
     1321 gccgctaccg gagacgctgc tcgcactcgc gcagcaggcg cgtgagctgc tcgacgccga
     1381 cgcggtccag gtctccctgt ccgcggccag catgctcatg atcgagccgc cggccgggcg
     1441 cgtccgccca cgcgacgggg cggcttcgat cgtccagcgc gacgacggcg gcctcaccgt
     1501 ctccgccgtt cgcccgggcg atggcgctaa gcacgcgtcc ggggtcgagc cggtccgcct
     1561 ctcgctcacc aactcggaga tggccttcgg tgagctgtct gtcgtaccgg ccgccgggac
     1621 gcattactcg gagcgtgaat ggggcttcat ccaccggatc gcagagctgg cagtcatcgc
     1681 catcagcgcg gcccggatgc gcgagcagga gcgccaggtc gcgatactgg ccgagcgcga
     1741 gcggatcgcc cgcgagatgc acgacagcct cgcccaggtg ctgggcttca tccatctccg
     1801 gctggtggcg ttaaaggcca aggtggcgcc catcggatcg ccccatatga acgacaacct
     1861 ggacgagctg accggcgtcg cccatgacgc ctaccgcgac gtgcgcgagg cgatcctggg
     1921 actgcgcgag tcgagccgca acggccgcag cctgttcgag agcctgcgcg cctacctcga
     1981 gcggtaccag caccaggccg cgatcgagac ctccttcgag accgacttcg accagccggc
     2041 aacgcttacg ccggccgccg agatccacgt catccgggtc atccaggaag cgctcaccaa
     2101 cactcgcaag catgcccgcg cccggcacgt gacggtgcgc gccacgacgc gcgatgggtt
     2161 cgtggcgttc gtcgtggagg acgacggtcg cggcttcaac ctggccgatg tggccctctc
     2221 acatgaaggc ggctttggcc tccaggcgat gcgcgagcgc atggagctga tcggcggcag
     2281 cctgtcgatc gattccgcgc ccaggcatgg gacgcgcatc acggcgctcg tgccggcggt
     2341 tgccaacggg gccacggttt ctgacggggc cacggtttct gacgggacca ccgcccgcga
     2401 ggaagtagcc cgtgtcacca gcaactgagg atcttccggt gcccgcccgc gtgctgctgg
     2461 tcgatgacca gccgctcttc cggcgcgcca tcgcgacgct gatcgaggag cagcccgact
     2521 tcaccgtgat agcggaggca agcaacggcc aggaggcgct cgaccgggcg cgcgcgctga
     2581 ggcctgacct cgtcgtaatg gatgtcgaga tgccggtgct caatggggtc gacgcggtac
     2641 ggctgctgcg cgacgagatg gctgacacca aggtcgtgat gctgacggtc tccgaggacg
     2701 aggactacct cttcgacgcg gtccgcttcg gcgccaacgg ctacctgctc aaggacctgc
     2761 agcccgagca gctgtacgag atgttgcgct ccgtcctgcg cggcgagtcg cccatctcgc
     2821 cagtcatggc cggtcgcctg ctggccgagt tccgcaggag ccacgcggac ggccatgcgt
     2881 ccgaccagcc ggccaacgcg cgcgcgggtt ttccggccga cggtcaacga tcccacctgc
     2941 cgccgcggcc cgttgcgccg caggctccgc cggaggagct ggcagccgcc gacctggcac
     3001 gcttccccct ggcgtcagcc gttgcccagg tcctcacgcg gcgtgagctg gagacgctcc
     3061 agtacgtcgc cgacgggctc tccaacaagg agattggcca gcggctgtcg atcaccgagg
     3121 gcaccgtcaa gaaccacgtc cacaacgcgc tcgagaagct gcagctcgag aaccgcattc
     3181 aggccgcggc atacatcgtt cgccagggca tcgcccaccc gccatcgagc aactaggcgc
     3241 gccggccctg ccgtacccgg ggatatagcc gccggcaatc gcaaatgtgc ccctctgggg
     3301 atggattcgc cggtgacgcg cgccgagatt ggcactcgat ggccagcgac tcgtccccca
     3361 aggcgcgcgt gctcgtgctc tacgaccatg cgctgctcgg tgagggactc gccagcctgc
     3421 tctccgccga accgggagtc ggtgcggttg cggtcaacgg ccgcgacagt gccgctcggg
     3481 cggcggccct gggcagccac cctgacctgg tgatcgtcga ggagacggag caggcccagc
     3541 cgtacgagcc gagtggctcg gcgccggtcg tgttcgtgcg cgtcgacgcc agcggggagt
     3601 gcccgttggg caagccgctc ggcgaccccg aggcaatcgt cgccctggcg cgcggcctcg
     3661 tcgaggcgcg cgccggcact taacggccgc ggccggcccg gtgtgcccta tttctacccg
     3721 gcggcggatg gcattcgagc acgtgcgcgc cgaccctgct cgcagggtct cgatctccgc
     3781 tctgtgcttt cccttccagc ggagcggggg tcggggcctc aaatgatcag gctccagaca
     3841 aaagagagat gagcaacacg aagacgatcc gcctggagcg gccgggcgca ccaaccgaca
     3901 agaccctaaa tcgcctcatc ggcgtcctga tcgcgatcat cgccatcggt ttgccggcga
     3961 tcggcgtcat ttactacctg gatcggcatg tcgatgccgg gccatccatc accggccgag
     4021 ccgtcatcac cgccgaagag gctgtgcgcc agtaccccaa tcagttggag acccgcgtgg
     4081 tgctcgccca ggcctacgcc gccgacgacc gcccggccga cgcaatcgcg caatacacga
     4141 tcattctggg tgccgagccg gttaacacga ccgctctgct cggccgcggc gacctctatc
     4201 gcaagaccgg ccagctcggc gcggcagcgt ccgactacca ggccttgatt gatgtggccc
     4261 gtgacacgga gatggccaac atcgaccgag cgcttcaatc ggcctactac gggctgggtc
     4321 tcatcgcgat gacgcagggc cggccggatg acgcggcaac gcagctcgcc aactcactcc
     4381 gcatcgaccg gacggacgcc gacgcactgg acctgatggg ccaggcgctc atcgcaatcg
     4441 gtgactacga gacggcggtc gaggcgctgc gcgacgcggt ggcctttgtg ccgaccggct
     4501 gggtcgatcc ttacacccac ctcgcgcagg cgtacgaggg tctggccgac cagggcggcg
     4561 tggcctatgc gaacggcatg gtcgcgctca atgatggccg gctggacgac gccgacgcgg
     4621 cactgcagcc gctggtcggt ggctcacatt cgcgcgacgc gctgatcggc ctcggtctga
     4681 ccgcagagca gcgcggcgat gccgccgcgg cagccgactt ctaccggcgg gtgctcgcgg
     4741 tagtgcctga cgacttcgca gcgctctccg gtttgagccg cgttggagta cccgcgacag
     4801 ccccgctcag cgggagcacc ccatgaccga catcgcactc acaccggacc tcgatcaaag
     4861 cctcccggcc gacgccggca cggcgccgcc cgacgcactg gggcctgagg atcggcgccg
     4921 acggaggcgc cgcctcgcga ttcttgccgt gctgtcggtg ctggccgtcg tcctgctggt
     4981 gttcagcggc tggtatctgc tgttccgcaa gccgatcagc ctgctgccaa tcccggggtt
     5041 cgatctgcca ccgatgcccg gttacagcta cagcctctat ggcatgggcc agccgacggg
     5101 catcgcggtg acgcccgatg ggtcgcgcat ctacgtgaca cagacgtccg gcgaggacgc
     5161 cgtgctgatg cttgacggcc agggccaggt catggccgcg ctgcagtcac cagacaccac
     5221 taccgaccac gtcttcgtct atatcgcgat caaccccctc acggccgagg tctacgtgag
     5281 cgatcggccc gccgccgacg tcctggtctt cgccgccgac ggcacgttcc tgcgcacctt
     5341 tgagccccca gcctcgctgg tgggatggca gccgctcggc ctgaatttca cgcccgacgg
     5401 ccacctgctt gtgaccgacc tgggagcaaa tgcggtctac gagttcgaca ccgccggaca
     5461 gctgctcaga acaatcggct cgaacggcat gttcaactac cccaacgccg ccgccgaaga
     5521 tgcgctgggg cggctgtacg tctccgacag caacaacggc cggctggtcg tcttcggccg
     5581 gacgggcgag ctgctaggcg tcatccggcg tggaccggcc gccggcgacc tgggcatgcc
     5641 gcgtggcctg gagattgacg accagggccg gctctacgtg gtcgacaaca ccgaccacag
     5701 catcaaggtc taccgaacct cggccgatcc ggtcgccctg ccggagttcc tgggcagctt
     5761 cggcgagcct gggatcggcg agggcatgtt ccgcttcccg aacgcggtcg cgaccgacac
     5821 acgtggtcgg ctctacgtgg ccgactggaa caacgacagg gtccaggtat ggacctactg
     5881 aagggacaca tgagtgagca caagcccggt cgtcgggcag gttcggctca aggcagagac
     5941 aaggaggtgg tcactttgag acggctggcg tctctcctcg cggggggcgc cctattcctg
     6001 ttgatcggcg ccatcccggc acttgccgac ggcggcccgc acacgctgac tggaaacagc
     6061 ggcacggcgg gcctcacctc ggatagttgc gcgggctgtc accgcacaca cacggcacag
     6121 gcaccactgc tgctcgtaga agaagagtca gcactgtgcc tgacctgcca cggcgacgct
     6181 ggtaacggtg caacgaccga cgtcatgaat ggcatccagt acgcaaccgg gacaacccat
     6241 tcggcgaccc cggataacga cgtcattctt ggcgccctgc gcggcggcgg tttcgccaag
     6301 gcgcgcatcg atacgagcaa cgccactcgg ctgacttaca cgcggcgctc cggctccgcg
     6361 gggaattaca catatccctt gacgcaggcc caggcggtgg gtgtcctcgc ggctggtgaa
     6421 acagtcacat cgaggcacct caacccggca gcatcggctg ccagcaccac ctggggctca
     6481 ggcgcgttgg gcacgaacac gacaggggca gcgatcacgc tcgagtgcac gagctgccac
     6541 aacccgcacg gcaacggcca gtaccggatc ctcgatccca ccccgggtga cggagacggt
     6601 ccattcgtcg aggccaccgc ggatgtcgtg gtaaccgatg tcctggagcc caatcgactc
     6661 cacaactaca ccgtcatcca gaagctgagc acgtcagcga ctttctcgtc cagcggctcg
     6721 attacggccg agggcttgga agccggttat ctgctctacg cggatcagat cgacgatgag
     6781 tacggcgtgt cggtgggcga ttacttccac cgcaaccttc cctggaacgg ccggacggat
     6841 tacaccgccg aggccgcagg tacgccaggt gctgtcatca ccaatcgcat gaacgacgcg
     6901 ccgaacggca agcctttgac gttcgacgcg cagatcaatg cctggtgcag ccagtgccac
     6961 acgcgctacc tggccgggtc cggtacgtac aacacgtaca gcggcgacac ggtcttctcg
     7021 tatcgccaca ccaatacctc caacaagccc tgcacgacgt gccacgtcgc gcacggatcc
     7081 aacgccgcaa tgaccggcat tgccgcgacg gcgctggacc cggccgacga gccatctgca
     7141 tcaagccgct tgctcaagat tgacaaccgc ggcacctgcc aggcctgcca tgacccgacg
     7201 gatacggtcc cagccggaac tttgctgggc cccactccga acgttccctg acaaaggtct
     7261 gagtctctct ggtgatgggg tcgcaaggcc ccaccaccag gggacagcca aacaccggga
     7321 agggcggtga gcgaagcaat gtcgcaagag caccaacggg cggctgcgct gcgtctgtcg
     7381 cgctcgctgt tcgttctgct cgcgctcgcg acgggcctgg cgctggccta cctgcccgcg
     7441 caacccgggg tcgccgtcgc tgaaacggcg gacaccgcaa gccctgcacc gggcttggaa
     7501 tcgtcgcctt cacccgaccc ggccggtctc gatcccaccc caagcccgac ccttgagccg
     7561 gccgcctcac cgagccctga tcccgccctt gagccgagcc cggacccgtc cacggcgccg
     7621 acggctgatc cgagcgccag ccccgtcgcg gcgtttgcgg tcatgcagca cgcgttcgcg
     7681 cgcgtcgcga cggccgcggc gccgccgacc tacctggcac ccggtgcacc cctgaccgac
     7741 gagccgcgct acgaggtctt ccgtatcagc ttcgatctga ccaacgccga cgcgctcgac
     7801 cgccaattca cgccactgct tgagttcagc tctgatggca cgacgttcgc acgtgttccc
     7861 gacgagacca ccggggcgga ggcggcgttc cacgtcgccc gcgaatgggt ggcgcagccc
     7921 aacggcggca cgaccatcgg gccatcgctc gtcattgagg cggcaggtgg cgtcagcggc
     7981 gccctggaac gcgtccgttc gagcggcctc aacccgctgc ccggtcagat cgtccgcccc
     8041 ggctcatcac tgagcgtgga attcagcgtc aaagccaccg cgggcgcggc atacaacgcc
     8101 acttatgtct tccggctcac cgacgatggc gccgcgctgc cgggcgcaag cgcggccact
     8161 ctgacgatgt cggcccgccc ggcgctccag ctgacacccg gccagcgctc cggcgtgccc
     8221 ggtgacgcga gcgcgcgcgg cgtgacgcgc tacgggctcc aggccagcag cacctccgtc
     8281 cacacgccgg cgttcgacct ggtgagcgac tcgtgcgtgg cctgccatcg cacgcacacc
     8341 gccgcgggca gcctgctgac cgcccagccg acgcagtatt ccctgtgcgc gtcgtgccac
     8401 gacggcacga acctgcccga catagccgcc gcctatgccg gcgtgccggc caatgacgcc
     8461 acggcgcgcg cctactacca gcacgatccg acccaggtgt cggggccggg caagaccaac
     8521 gactgcgcgg aatgccacaa cccgcacaac atcaccgacg cggccgcggc ggagacggtc
     8581 agcggttgga cagccagcgg catgctttcg ggcatcggcg aggtggagct cgagtaccag
     8641 ctgtgctacc gctgccacgc gagcgcggcg accctgccgt ccaacagcgg ccagccgccg
     8701 tcgcgctatg cgctcgacaa ggcactcgag ttcgacccgg ggaacgcctc gtaccacccc
     8761 atcgagtcgc ccggcacgaa ccagacggcc gcgctggcga acagcctggc cgcgtctggc
     8821 acatcgccct acaagctctg gtcgtggcag accacgagca ctattcgctg cgtcaactgc
     8881 cacgccgacg cgcgcagagc cgacgctgcc ctgaccccgc cggggagctc ccttgccgcg
     8941 agcgcaagct tggccatcca cgccagccct gagcgcggga tcctgatcca gccctaccag
     9001 gacaggtcgc tcaacggtcc catcgagccc tacgcggcgg ccgattttgc gctgtgctac
     9061 gtgtgccaca gcgaagcgcc cttcgtggac cagacgggcc aggtgcggga cgggcagaac
     9121 ggcaccgtgg cggacaccaa cttccgctac cacggcgtgc atctgtcgaa cccggacgtg
     9181 atgagcgacc gtggcttccc gggcacggat atcgacacgc ccggctatgg cgccggcttt
     9241 gcgatctgcg ccgagtgcca cttccgcatc cattcgtcgg ccttcccggc tgatggccgg
     9301 acgccggggt cgcgcttggt gaacttcgca cccaatgtga cggcgcccac cggcggcgtg
     9361 ctcagctggc agcccgtgac tgggacgagc gccggcagct gcacgctgaa atgccacggg
     9421 gcgccgcaca acaacgtgga gtactgagcg acccgcgcgc ctatatccaa cggtaccccg
     9481 ccatgagcgg gaggcatgcc cgcgcgggcg cctgtatcca aatcgtgacg gcaggcggat
     9541 ggtccacgaa atcgctcaaa tcgatcattt ccgcatgagc agactcacct ctggcaccca
     9601 ccgccgcaca tccatccagg ccctcctgct ggtcgttacc ctcctcggca cggccgccat
     9661 tgccacggcc tgcggtggaa gccacgccac cagcccgacg aaccaggcgg agccttcgat
     9721 ggccgccatg gacacggcgc acgcagcggc atgggccgct cggcccggct acacccaggt
     9781 ctcgcagcgc accgaagagg cttaccagta cgcgatggag catccgctca tcgtgcagtg
     9841 gatgccgtgc tactgcggct gcggcggcat cggtcacggc agcaatctcg actgctactt
     9901 cgagccgagt gccggcggca cgatcgtctt cgaggagcac gccagctact gcgagatctg
     9961 cgtcgacatc accctgcgca ccaagcagct tgccaactcg ggcgcgacgc ttgccagcat
    10021 tcgcacggcg atcgacgccg agttcggcgg agctggcccc gggaccgaca cggaacggcc
    10081 gctgtgatgg atggctcccc cgtctcagag cacgagctgc tgagccgcct cggcttgtcc
    10141 gacggcgcca gcgaggacca agtcgagacc gcgcatagcg aggtcgtggc gttcctcgcg
    10201 ggcgcgcccg cagatctgcg tgactgggcg cggagccagc tcgctgcggc cgatgaggcg
    10261 tatgccctcc tgtccgaccg cgccggcagc ctgcagtcac gcccacgacc cgcggccaag
    10321 ccggttaagc agtcggtcca accgcctagc cgcacgctcc ctgcacgcgc catacaacag
    10381 ggcgtggccg aggagccgcg ccagccgttc ctgagccgca tcggccgcgt cgggcgcgtc
    10441 gccatcacca ccatcgccct ggccggtgta ctggtcggtg cctacgttgt ctacgcgtcc
    10501 gacctgccca gcgtgcccgg cctctccggc acgcccgccc ctgggggcca gcagacagcg
    10561 ctcgacaccg cgcgggtcgc ggagctgatg cagacgatcg cagataaccc aagcgacatc
    10621 gccgcgttcc aggagctggg cgacctgtac ttcacggcgc gcgattacca gaccgccgcg
    10681 caatgggaac agcgggtcct cgacatcgat ccgaacgacg tcacggctca tctggcactc
    10741 ggggccgccc agtacaacct cggcaactcg gccgacgccg agagccactg gcgccgcgtg
    10801 atcgagctca accccaatga caccgacgcc ctggtcgagg cgcactacga cctgggcttc
    10861 atgtacttca gcgccaaccc gcccgacgtc gacaagacga tcgccgagtg ggagcaggta
    10921 atcgagctgg ccccggactc ggatatcgcc cagaccgtcg cggctcatct ccagacgctt
    10981 gagcagtgga acgcctcggg ctcgccgacg acctctggct cacccagccc gacctcggcc
    11041 gccaccccga ccccggcggc cacggcgcaa ccaacggcca ccgccgggca atgaccgagc
    11101 tcagcctgct cgtggcgttc gtggccggcg tggtgtcatt cgcctcgccg tgctgcctgc
    11161 cgctcgtgcc ggcgtacctc ggctatatgg tcgggtccga cccgaccgcg gccatccgtc
    11221 gtcgtgacgc gctcgtccaa tcactcttct tcgttgccgg cttctccgtg gtcttcatca
    11281 gcctctgggc ttcggtcggg ctgatcggct acgcgctgcg cgatcaggcc tggatcctgc
    11341 gccaggtcgg tggggcagtg ctggtcttca tgggcctcca cgtggcgggc gtcatcaatg
    11401 tcgcggccct gtaccgcgag atccgcctgc cgctgcccgc ccggctccag ccggccggca
    11461 tgggctttgg cgagcacccc gctggcgcgc ccagccatgg ccgctcggcc atgttgggcg
    11521 tggtcttcgc ggccggctgg acgccatgta tcggccccat cctcggcggc atcatcggcc
    11581 tcgcctcggt cagcacgagc gtcgcgcagg gcacgagcct cctggtcgcg tatgcagcgg
    11641 gcctgggcgt cccgttcgtg ctgatggccg tgggcgcgac ctgggtcagc ccccgcctgg
    11701 gtttcctgcg cacgcaccac acggcggtgt cgcgcgtgac cggcgcgatg ttgatcgtcg
    11761 tcggcttcct gatgatcacg aacactttcg cccgactttc cggcctcatt ccagctgctc
    11821 tgtgaaggga tcgcacgcat gactcagctc tcacgcacga ccgccatcga gcgccgcgcg
    11881 gccgcagcca gcatcggctc cgacttggac tcggatgcgg tcttctcgcc cagcggcgcc
    11941 ctcgaatggc tgtgggcgac attcacgtcg atgcgcctgg ccctggcgct gatgctcgga
    12001 ttggcgctgc tcgcgctggc gggctccgtg atcatccagg cgccggccgg cgtcgccagc
    12061 gatcccgggg cgtacaacgc ctggctgcag ggtctcaagc cgcgctacgg cggcttcacc
    12121 acgatcttcg acaagctcgg gctgttcaac gtctttgcct cggtctggtt ccgcggcctg
    12181 gccgtggcgc tcaccacctc gatcctcgcc tgctcgatca aacgcgcgcc gaccctttgg
    12241 agagcggcgg ttcacccgcg cacgcgcatg agcgagacgt tctacggatc cgcgccctac
    12301 agcgagaaca tcgccaccag gatgtcgacg gccgacgccc tcgccaccac gcagcgcgtc
    12361 ttcagggcca accgctatcg cacgatcgtc gatacggatg ccaccaccgg catcacccac
    12421 ctgtacgtcg accagaaccg ctgggccccg ttcggcacgg tcatcgccca cctggcggtc
    12481 gtcatcgtcc tcgtcggggg catggtcgga tcgatcatgg gctaccgcga cgaaggcttc
    12541 gccgttccag tcggcagcac cgtggctgtg ggcggcggga ccggcctgtc ggtcgaagcc
    12601 gtctccttct ccgactccta ctacgacaac ggcgccccca gcgactacgc cagcagcctc
    12661 gttgtctatc gcgagggcac gccggtcgcg gccaagacga tccgggttaa cgacccgctc
    12721 gaggtcgatg gcaccgcctt ctaccagtcg ttcttcggcc cggcggccgt catggccgtc
    12781 agcgacgcca gcggcgcgtc gctctaccag ggcggcgtgc ccctgctgtg gtcgtccgac
    12841 gatgacaccc ggcgcatcgg ccagctggcg ctcagtgacc agaacatcct cgtctacgtc
    12901 gtcggcgtcg cctcgggccg cgtcgatgcc aacatccgcg ccggccagat ccagctcgag
    12961 atctaccgca ccggcgaaca gcagcccatt gcgatcgaga cgctggatca gggtgagcca
    13021 ctgctgatcg gcggcctgac ctacacgttc aagcgtgagc agcagttcac cggactgatc
    13081 gtcgcgcgcg atcccgggca gccactcgtc tggctgggcg cgctcgcgct cgtcctgggc
    13141 tgcttccttg tcttcctgtt ccccaaccgg cgcatctggg cagccgtcaa ggctggccct
    13201 gacggcgcgc aggtctcact tggcgcgacg gcccgtcacg acgcggcgtt cgcaccggag
    13261 ttcacaaagc tcaccgaaca ggttcgcctg gcattcgccg gcgcggccta gagaaaggaa
    13321 gggatcccgc gatgttcaag atgtcggagt acagcttcat ggctggcctg atctcggtcg
    13381 gcctcgccct gctgtgttac gtggccgcgt tcgcatctgt tcgcctggcg cggcgccagt
    13441 ccgctcttgc tctcgacggc gggcacgccg gctcgaccat gctgagcggc cagtcggtcg
    13501 aggtcacgcg cggccccagc agcctcgccg gctacggcac gctgatcgcc tggctggcgc
    13561 tgatcttcgt cagcgcagcg ctggtcttcc gcaccatcgc cgtcggccac gggccgttcg
    13621 ccaacatgta cgagttctcc atcgccttcg cgtggggcgt cctcgcggcg cacgtctact
    13681 tcgagcggcg ctaccacctg cgcgcgctcg gactgttcgc gctgccggtg gcgctgctgc
    13741 tgctgctcta cgcaacgacc atcccctcga catcggatcc gctcgtgccg gcgctccaga
    13801 acaacctgct cctcaccacc cacgtggcgg tggccatcgt ggcctatggc ggcttcgcgg
    13861 tggcgttcgc cgccgcggcc ctgcacctga tcgcgtcgta caccggttcg cgccggctgc
    13921 cccggctcga gctgctcgac gagatctcgt atcgcgcggt ggtcatcggc ttcccgttcc
    13981 tgacactggt catcgtcctg ggcgcggtct gggccaacgt ggcctggggt cgctactggg
    14041 gctgggatcc caaggagacc gcgtcgctgg tgacatggct catctacggc gcctacctcc
    14101 acgcgcgcgt cgtccgcggc tggcgcggca accgcgccgc gtggctgctc gtggccgggt
    14161 tcgcggccac gcttttcacc tacttcggca atctcttctt cggtggcctg cacagctaca
    14221 gcgggctgaa ccagtgagcg cgactcttac tgcctcggcg cccgccccag ccggcgccga
    14281 ggccaccacg ccatccagcc gccggcgcaa cgccctggcc accctggcga tcctcggcat
    14341 caccggcgcg ctcatcctcg ccgccgcgta cctctccaac ccgcgcctgg gggacggcag
    14401 cggcaacttc accccggtca ggcccgatgg tcagggcgtc ggcgctgcgc ccgtcatcga
    14461 cagccccgcg ccggatttca tcgcggctct ggctgacggt agccagatgc gcctgtccga
    14521 tctgcgcggc aagccggtgt ggctgacctt cggcgccagt tggtgtcagc cttgccgggc
    14581 ggagaacccc gacatttcgg ccgtctacgc cgccaacgtc ggcaagattg cgatcgtcca
    14641 ggtgtacatg caggaagacg cgctcacggt caaggactac accgaccgcg tgggcatcac
    14701 ctacctgacc gtgcccgatc ctacccaggc gctgtcggcc caataccgga tcctgggcat
    14761 cccgtcgcac ttcttcatcg acgcgaccgg cgtcctgcgc cagatcaagg tcggcagcct
    14821 cgatccggta tccatgcagg cggcgatcga cggactcagc cgctgaccct gcggacccgc
    14881 tgaatgaccg acgccggcgt cccgaccgac cagcccgacg accagtctga cgacctgtcc
    14941 gacgacctgt ccgacgagct gccatcggcc gcgatcggcc tgagcgaccc gccgcgcaag
    15001 cgccctggcc gcctgcgccg cgtgctccgg cgcattccct tcccgcggcc gcgatcgcgg
    15061 cgtggcgcat tcgtcctgtt catcctcgtc gcgggcttcg gcgcggcgct gaccgtaggc
    15121 gcggtcgcga ccatcgccta caccgattcg gtcgccttct gcgggacgtg ccacaccatg
    15181 gcgcccgaga tgaagggcta cgagatcagc gcccaccgca acgtcgcctg cgtcgaatgc
    15241 cacaccgagc ccggcctcgc cggctggttc aaggccaagc tgaacggcac ccggcagctg
    15301 atcgaggtga tcgccggcac attccccaca cccatcccgg cgcccgacca cgcaatgctg
    15361 ccgtcggtcg aggtgacctg caagcgctgc cacaacgttg gcccgttgct ggccggcggc
    15421 ggacccatcc gcctcgtgct gcacgaccgc tacgcgctcg atccgcccaa cacgcgtcag
    15481 tcggtcgcct tggtggttcg gcccaacggc ttcggcagcg gcaggccggc agtcggcgtc
    15541 cactggcacg tggagtcgga cgtggaatat ctcgcgcccg acccgcgcgc gcaggagatc
    15601 gactacgtaa ccgtccggcg cagtgacggc tcattcgagg agttcatcgc ttccggctcg
    15661 gtcacgcagt cggacaacgt cggcgtcgac gtggcgtcgc ttgcgcgcga gctgacgccg
    15721 cgccggatgg actgcatcga ttgccacaac cgcgtgggcc acgagctgcc caccgtcagc
    15781 gcggcgatcg atgcccagat ctcggcgggc cagatcagca ctgacctgcc gaacatcaag
    15841 caacagggcg tcgagcggct gtcgctgacc tacgccacgc tcgaggcggc cgatcagtcg
    15901 atcgccggcc tgcgctactt ctactcaacc aactacccgc tggtggcgcg cggtcgttcg
    15961 gctgagatcg aggccgcaat tgcgagtctg cagacgacct atcggcttgt cgccacgcct
    16021 gagatgggcg tcgccgcggg cacgtatccc aacaacatcg gccaccagga cgcccccggc
    16081 tgcttccgct gccacgatgg tgcgcactac aagatcgtcg ccggcaaggt gaccaacgag
    16141 acgatcccgt cgcagtgctc gacgtgtcat acgttccccc agatcggcca atacgaatcc
    16201 ggcgtgctga tcggccagcg gccgtcgaat cacgacggac gcctgtgggt cttcgaccac
    16261 aagctgtcgg tcagcaccgc cgacccgacc ggccagagct gcggcgcctg ccatacccgc
    16321 acctactgcg agaactgcca cgcgacgacg gccatcaacg tgtcgcacga cgagatggtc
    16381 accaaccacg cggctgtgat ccgcaccagc ggcgcccagg cctgcgttta ctgccacgca
    16441 ccggcctatt gcgcccagtg ccacgccgac ccggtgatgc cgctggggcc cgccgggacc
    16501 ggcgcgcgac cgcttctgcc ggagatcgcc tatccagtgg cgaactcgcc atgagcgccg
    16561 cagacaccgc cgcggccacc gagtggctga ccattcgcga ggcgagcgcc ttggttggcg
    16621 tctctgtcgc gacgctgcgc cgctggtgcg atgccggccg cgtccacgtg ttcacgacgc
    16681 ccggcgggca tcgtcgcttt gaccgcgcgg atgtgatgga gctgctgccg gccgtggaca
    16741 tccccgcccg gctcgatggc ttcgcggaca cgtcgttggc catcgtggag ggctaccggc
    16801 gcgccactcg aaagttcccg tacctgccgc gcaggctgcg cgagctgccc gaagacgagc
    16861 gagtgcagct gcgccagcat ggtcggcgca tcgtggccgc gctcgtcgag acgctcgatg
    16921 cgccgtcaaa tgaggaggcg gtccactgcg gccaagcccg cacggcagca gctgcctgcg
    16981 ggcagatagc gcggcgctcc ggcttgtcgc tccaggacac cctcgcgctg tttgtccggt
    17041 tccgtgcgcc cttcctccat gagctcggcg cggtgtgtcg caggcgcggc ctcaccggcg
    17101 cggccgccac cgccctgctg gagcgcgcca gtgatctgat ggatcgcatg ctgccggcga
    17161 tcgtgtccgg ctacgaggcc gccgaggcgc gccggcccta ggcgccgtcc cgcgtcttgc
    17221 ccgcgcggtc acgctcgacc agcggcagcc ggcgcgggtc gcgctcgaaa ccttcgcgga
    17281 cgcgggcgtc gatcagccgg gcgcgcttga tggagcgcga gccgtacttg tggcgcacct
    17341 cgtcggccgc ggcctgggct ttgcgccgcc gctcgtcgcc gccaccgaaa agcgcaagct
    17401 ggtgttcctc cgtcagcccg ctcgcggcga cgcccagcag ccgcatgacc ttgcccttga
    17461 cctctttcct ggtcagtgcg acagcggtgc gccagatgag gtccgtcgaa tcggtcggtt
    17521 catcgagcgt cttctgacgc gtgatggtga caaagtcgcg gtcgcggatc ttgacgccca
    17581 ccgtccagca catgatgtga tcgtcgcgca gtcggcgggc cacgccttcg ctgagcgcga
    17641 gcagggtctg ctcgagcttc tcccactcgc cgacatccgt atcgaatgtc gtctcctggc
    17701 tgaccgactt ggccgcgtcg tgaccgccga cggcggtctc acccacgccc tgggcgcggg
    17761 aggccaggtc accaccgtgc ttgccgaaac gccggaccaa tacgtcctgc ggcagggccg
    17821 ccagatcacc gatcgtgcgc acctggtagt cgaccagggc cttgcgcgtg ctcgcaccaa
    17881 cgccccacag ccgctcgatg gcgagcggcg cgaggaagtc gcgctcggtc cctggtggca
    17941 cgacgacaag gccgtcgggc ttcttcaggt ccgacgcgat cttggcaaca aggcggttcg
    18001 ccgccacgcc gacggagata gtcagccccg tttcgtcgtg cacagccttc ttgagcagtc
    18061 gtgccacggc ctcaccggtg ccgtagaggg tctccgtccc ggtcacgtcg aggaacgcct
    18121 cgtcgatcga cagctgctcg acgagcggcg agaaacgctc gaggatggtc atgacctgct
    18181 tgctcgcggc gctgtacttg cgcccgttga cgggtacgaa gatcccctcg ggacagaggg
    18241 cggccgcggt ccgcagcggc atcgccgagc gcacgccgaa cacgcgcgcc tcgtaggacg
    18301 ccgtgctcac tacgccgcgg tcgtttggtc cgccgccgcc cacgatcacg ggcttgccgc
    18361 gcaacgacgg gtcgtcgcgc tgctcgacgg ccgcgaagaa cgcgtcgaga tcgacgtgca
    18421 ggatcgtccg cgtctggcgg tcgctcaccc caccatcatg cgcgccgggt gtgccaactc
    18481 acatggcgcg gggcgcgagg acgtcagccc ttgggctcga cgtccaccgt cacttcgggg
    18541 gtcaggccgg ggtagacctt gatcgcgacc ttgtacgtgc cgagcatctt gagcggctcg
    18601 tcgaggtcga ccttgtggcg gtcgatctcg atgcctcgcc gcgccagcgc gtcggcgatg
    18661 tccttggccg tgatggagcc gaagagcttg ccgccctcgc ccaccttcac gcccagcgtc
    18721 aatgtcgtcg acgagatgcg ctgggccgcg atctcggcct cctcgcgctc gcgcttgcgc
    18781 ttgtcctcgc gtgacgcgat gtcgtgctgc caggcacggt aggcaccgcc ggttgccgat
    18841 acggccatct tgcgcggcag caggaagttg cgcgcgtagc cctcggcaac ctctttcatc
    18901 tcgccgctct tgccgagctt gtcaacgtcc gccgtgagaa tgactttcaa gccgggtttc
    18961 cttcgctgct gggcatgcgc gcagatatag ccgcgcgcac tttctgatcc actccacttt
    19021 cactaatgcg ttgcgcgaca cggtcgagcg cgtccgggac acgcgccatc atggcccgca
    19081 gaggccgtgg caccacacgc cgcacgcgcg ccaggtcgtc ttcgagctcc gacgccggga
    19141 tgcccagctc gaccagcttc tccgcgacga gcccttcggg caggccctcg aggaagacgt
    19201 caatcgccag caccatcacc gccacatcgt cgaccgcgcc cagcaccggg atccactcgg
    19261 gcacgatgtc gagcggcgag acgatgtacg cgccggctag cccgagcagc gctttacgtg
    19321 acgccggcac gcgcggatcc gacgcgagcg accagagcag ccgcgcgtag agcggcgcct
    19381 tgccggccag cggcaggaag ggcaggagat tgagcacgcg cagcaggcca ccgccgcgac
    19441 gtcgagatcg ggccatcgga cgagcgagta tatgcgagcc caccggttca gccccagttc
    19501 cctgatcccc gctttggagt atcttcacgg cgtcagctgc ggaaaagatg cggcacccgt
    19561 ggggaagtgc cggcggctgg aggtaatcgg tgcccgtcct gaatcgacct tccgcgcgcg
    19621 cgaccgtagt cgcgctcctg ctcatggccg caatcggcgt gggctgctca agctcggccc
    19681 cgacgggcaa gatcggcggc acggtcagcg tcgtcgcctc atggagcggc gccgagcagg
    19741 aagccttcat ggccatggtc cgccccttcg agcaggagtc gggcgtgacc gtcgaataca
    19801 agggcacgcg cgacctcaat ggctacctgt ggcagtcgat cgcccagggc gatccgcccg
    19861 atgtggcggg cctgcccggg ccgggccaga tggccgagtt cgcgcgccat ggctcgctca
    19921 aggacctcgc tacagtcatc gacgtcagcg gctacaaggc cgacaccgtg cctgccttca
    19981 tcgacctggg cacagtcgac ggcaagctcg tcggggtgtt catcaaggcc acgctcaagg
    20041 gcctgatctg gttcaacccg cacgactgga ccgtcggcac cccggacaac tgggtcgagc
    20101 tggtcgccca ggcgaacgtc gcccggcggg gtgactccaa gacctggtgc atgggcctgg
    20161 ggtcggacgc aacttcgggc tggccaggca cggactggat cgaggacatc ctgctccgtc
    20221 agagcggtcc ggacgtctac gacgaatggg ttgccggacg gctgccctgg accgcgccgg
    20281 aggtcaagaa ggcgttcgag cttttcggca gcgtcgttgc cgacaccgac ggcggcgcgg
    20341 cggggatcat cgccacgaac ttcggcgacg cgggcaacgg cctcttcacc gatccaccgg
    20401 gctgcctgct ccaccaccag gcgaccttca tgacatcgtt cttccagacg cgcgccggcg
    20461 cgcgcgatgg cgagtacgac ttcttcccgt tcccgaccat cgacggtcgc ttcgccaaca
    20521 acgtcacggg cgcaggcgac ctcttcggca tgttccgcga cacgcctcag tcacgggccc
    20581 tcatgtcgta cctcatgacc ccggatgcgc agtcgatctg ggtggcgcgc ggcggcgcgc
    20641 tgtcagtcaa tgtccacgtc aacaactatc ccgacgagat ctcgagccgc gcggcggcga
    20701 ctctcgccaa cgcgacgcgg ttccgttacg acgcgagcga cctgatgccc gagcaggtca
    20761 acgaggcgtt cctgcagggc gtcattgact atgtccgcga cccggcggcg ctcgacgcga
    20821 tcctggcgca catcgacgag gtccaacgca gcagcatcaa caccgagcca gtacgacctt
    20881 gagcctccga aacctgacat gaagatcgcc gccttcgccc ggccgcgcca gatgtccgac
    20941 atcaactggc cgcggctgct cgcgacggcc ctcctggccg cgagcgtcat cagcgtcctc
    21001 gccagcatcg tcctgtgggc cggccagcgc ttccagccgc cgcccgtgtc gttcatcgac
    21061 agccagctcg tgttcgtggc cgagatgttc tacgcggtcg cgtatgcggc catgggctgg
    21121 ctgctcgcca cgcggctgcc gggcaacttg ctcggctggg tgttcctggt gctcggtctc
    21181 gggatggccg cgcagctgct cgtgaccttc caggtccagg agggccacga ggccttccgc
    21241 cagctgagct acccgctgct ggtcgcagcc tggatctcgt cgtcggccca cctgccgatg
    21301 ctggccgtgc tcactaacgt ggtcttcctg cgcttcccca ccggccgacc actgacgccc
    21361 cgctggcgtg gcgccggctg gctcgcgttc ttcggcgctg tgctggtgat cctcggcatg
    21421 ggcctgcagc cgagcggcct gacctggtat ccgtcgctga ccaacgtctt cgccgcgccg
    21481 tttgcctggc ggccgctgct cggcccaatc accctgctcg gcgtgtcgat gctgatcgtg
    21541 ggcgtgacga tcggcaccgc gtcaatcgtg gtgcgctacc accgctccaa cgaccgcgag
    21601 cgcgcccagc tgcgctggat cgccgtgtcg gtggtcctga tcgcgctggc cggtatcccc
    21661 ttcgtcatcg tgcgctacgg cctccaggcc gactacgcca cgggccacgc gctgctcgcc
    21721 acggcacttg tcgccggctg cctgctgccg ctcgccgccg ctgtggcaat cctgcaccac
    21781 cgtctgtacg acatcgacct gatcctcaac cgggcgctgg tgtacatccc gctgacgggc
    21841 atcctgggcg ggctctacgc ggccggggtg gccctcttcc agcgcctctt cgtcacggtc
    21901 acgggcgaca ggtcggacgc cgcggtggtc atcaccaccc ttgtgctggc gggcatgttc
    21961 acgcccatca agaacagcct ccagagcttc gtcgaccagc gcttcaagcc ggccggcgcg
    22021 acgccggccg ttcatcacga catccacacc gtcgagcagc gcctcgcgca gctggagcac
    22081 cggctggaac agctggaggg ccccgaaagt tcagcacggg atgtgactcc cgactgacgg
    22141 ccggtctccg tcctgcccta tccactgcga actcaaccta aacgactagc actcccgaca
    22201 ggccctacat gggacggcag gcggatggag tgcggactgc ccgtcggcga acctgcgctc
    22261 acacaaccgt cattgctagt tagtgggtgc gcaggtggcc acggatgtcg tcaagcgaac
    22321 gccgagctgg cgcatattga tgtgggtggg cgtcgtgggc tcggtgctcg cgtgggcctg
    22381 ggtctggtac ctgggtcggg ggcccaccgt ggtcatgctg ctcttcgcgc tcgccgcggt
    22441 cgccttctcc taccgcgcca acgccggatc acgctgggcg ctggtcgggc tcatggtcac
    22501 cggcttcgcg atgttcctcg ccagcctcta ctggctcgcc gcgctgtaca ccagcggtgg
    22561 cccgatcacc gtggccgacg cgctgacggc gtcgttcctg ccgctcgtcg ccgcgacgtt
    22621 cctgctggcc ggctcagcca ccggcttccg ccacacccgc gcggcgtagc tcaccccgct
    22681 gatctcgcgc ggcgcaatcg gcttcgcttg cgggttgaca ttcgaacagg cgttcggtta
    22741 cgatcacgcg tggaaggagg cgctgtgacc aaacacgacg ccgcccgcaa gcagcggacc
    22801 atcgaataca tcgcgtcaac cacccgccag cgtggttatc ccccgtcggt gcgcgagatt
    22861 gcgcaggcag tcgacctggc gtccacgtcg gccgtccatc accatctcca ggcgctcgag
    22921 cgcgagggca tgctggagcg cggcgcgagt cattcacgcg ccctgcgcct gacgcccagc
    22981 gcatccattc gcgccggctc aagcgggccg ctcccggcac aggtcaccag cctggattcg
    23041 catgcactgc cggtcatcgg tgagatcgcc
//
LOCUS       DATBRX010000005        17314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2572390, whole genome shotgun sequence.
ACCESSION   DATBRX010000005 DATBRX010000000
VERSION     DATBRX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 17314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17314
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572390"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2175
                     /locus_tag="VIF08_00240"
     CDS             <1..2175
                     /locus_tag="VIF08_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HEY8134439.1"
                     /translation="GVRTPMQWSADRNAGFSDANRQRLFLPVITDPEYSYEAINVDAQ
                     QANPSSLLWWMKRLIALRKQYQAFGRGSLTFLTPENRRIVAFVREFGDERILVVANMS
                     RFVQHAELDLTPWRGLIPVEMFGRVEFPRIEERPLFLTLGPHSFIWFALEADPSGRGH
                     EIRPVEEKAPIISGPADLERLLSGGARALASVLPGYVRARRWFRSKARRIKGIALRDA
                     LRVPFDGSADIQARLAIFDVEFTEGEAETYVLPLALAPAERVEAALAPTPQAIVVTVL
                     PDGEGGAAWTLYDAMYEPAFATGLLDAVSGKRRISGRRTELTGSPTPALRRLRGGRGA
                     ALTPAVGRAEQSNTSVVFGDRLILKLFRCLEPGANPEIEIGQALTDAAFSHTPALGGW
                     LSLSGRREPQALGVVQEYVANEGDAWALTLDAVSDFYERAAATDAETEARAEIVPEMT
                     GDYLYFARLLGTRTAELHRVLAGRDDSAFSSEPFSALYQRSTFQNLRNQASGTIALLR
                     RRLGDLPDDARPAAEQVIASSDEILRRTRELTQARIGAVRIRTHGDYHLGQVLWTGKD
                     VVIIDFEGEPGRPLSERRHRRSALADVAGMLRSFHYAAFGTLLNPRLGGAVRPEDIAR
                     LEPWARAWYATVSAAFVDAYLEGAAGQPFVPRARAEQVALLDLAMLQKVLYELTYELN
                     NRPDWVSIPLRGLLALLGTDDGHVPTAPAAPAAPEAAVPDDA"
     gene            2180..3361
                     /locus_tag="VIF08_00245"
     CDS             2180..3361
                     /locus_tag="VIF08_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8134440.1"
                     /translation="MAQPQQARRQPEPHWGLRSALVTLGVAALVMGVAVALKALLLSV
                     SNANAGFIVYIPAVAVVAWYRGLLGGVLATLLGALADSVLFVPALFVAAADLSSVQVR
                     LLAYLAGGTAVSYLSYRLRSERDRARLEATERRHALQRAAQMSDELGRIAANERRASE
                     LRDAFNSIVSHELRTPITAIYGGAKLLARRDRPLDEATRQELIEDLEGEADRLYRLVE
                     DLLVLSRSEYESLERFDDPVAIAHVVRRVTLSEQVRWPGARFAFSATTTMTVRGDETY
                     VEQVLRNLLSNAAKYSPAGTTVEVVLDETPEGARVRVLDSGAGVRTDEVDRLFQLYYR
                     SPETAAKAGGAGIGLFVCRALVEAMGGRIWAAPRPEGGSEFGFILRRYADDDAPRSEA
                     T"
     gene            complement(3358..4065)
                     /locus_tag="VIF08_00250"
     CDS             complement(3358..4065)
                     /locus_tag="VIF08_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEY8134441.1"
                     /translation="MTIVGRLTDASNASFLCRIGADTLFDPDEWPDHELPPGPLCVYK
                     PTRGERPLHDFPDGTLGRREVAAFLLSEATGWAIVPPTVLRDGPFGEGAVQAWLETDP
                     GVDVVELIVGPDPRLRPMCVFDLVANNADRKGGHILPLPDGTLRGVDHGICFAVEPKL
                     RTVLWAWRGEPFDESETAALGRVAAALAGDLGAALTELLTRDEVRATAQRVAALLEHG
                     RFPEPDPARPAVPWPPF"
     gene            complement(4142..4786)
                     /locus_tag="VIF08_00255"
     CDS             complement(4142..4786)
                     /locus_tag="VIF08_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEY8134442.1"
                     /translation="MPRRVYSFDRPDRFIAGALGTPGQRTFFIQASKGSQQISVALEK
                     TQVALLAERIAALLLALREGGVTIGDDLPGPAPELAEPVVEQFRVGTMTLGWDGEVGR
                     VVIEAREIGEADIDEADIDEEADADDADEDEMEAAEGAADEGIELRSLAGDMDDPRDV
                     VRVELEPQAALSFASGALEVVRAGRPPCPLCGAPLDPTGHFCVRRNGHANELLN"
     gene            4848..6332
                     /locus_tag="VIF08_00260"
     CDS             4848..6332
                     /locus_tag="VIF08_00260"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY8134443.1"
                     /translation="MQFRVVSSLEGEHLDALIVPVFKEGDAASNTPADVRDTAAWVAR
                     EHGDTKMFAPITHLQSSAESSTRLIVVPAGQRADFDVARSWQFASAGVRGLWQSTATR
                     IAICLESDALDAQEAVQGAVEGVIYAMWRPESHRTTEKDRQLPPLEEVLLVIGGTASD
                     YEAAIGRGTFVGEAVNWSRGVANEPANLMTPTHMAQAAREMASQTSLKVEVLGEDEAR
                     DLGMGSYLSVARGSDEPAQVVVLRHHGRPGDGYDLALVGKGITFDSGGISIKPAEDMH
                     LMKYDMSGAASVLAAAGVVARMNLPLNLIAVAMCTENLPGGHATKPGDVVTSMSGKTV
                     EVTNTDAEGRLVLIDGITYAQREGAKRVVDVATLTGAVRIALGRLYTGVLGRPQSFID
                     AVLGAADRSGERMWPLPLDDEHREEMKSEVADLRNSSGGRLGGTILGAAFIDAAVDAE
                     TEWAHLDIASTAWYEDDRPFSPKGPQGAAVRTMVELATTIARAG"
     gene            6535..7740
                     /locus_tag="VIF08_00265"
     CDS             6535..7740
                     /locus_tag="VIF08_00265"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HEY8134444.1"
                     /translation="MRSSLKMRVLRAGAAALLLGLTLAPVALAQGPLELTTQYPAVTA
                     DPGSTVKFNVIAITDVATRVDLSTTAPTGWTVRMRGGGSTVSAVNTAPGPITEGSSTN
                     QIAGAFTVEIDVPIDVAPGVNHVVVTGRTAGGATASLSLDLTIEAQAAGAVSLTTNFP
                     NLRGSTATAFRYNVLLNNDTNQQLTFGLEAQGPAGWTVEAKPAAEQGATTTTVDAGSN
                     STIQVTADPPADAVAGIYDIKIIAQGGPEPVELDLTVEITGSYSLGLNTADERLNATA
                     TAGSKTTLELVVTNTGSAPVANVRLTATPPRGWKITFDQETIPVIQPGVDNGVKVVAT
                     IEPGGNAVAGDYVLTFRATSGEESTATDTIDVRTTVETSPIGLVIGIGILIVVAAGLF
                     LVFQRYGRR"
     gene            7727..8728
                     /locus_tag="VIF08_00270"
     CDS             7727..8728
                     /locus_tag="VIF08_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8134445.1"
                     /translation="MAVAEPAAHTTAGQPQPVIKTRALTKRYGNFTAVDKLDLSVRPG
                     EVFGLLGPNGAGKTTTILMLLGLSEPTSGSARVLDMDPARDPIAVKRRVGYLPDAVGF
                     YGAMTGRQNLQYTARLNGIPRKAADDHIDELLERVGLTNAANSKVETYSRGMKQRLGL
                     ADVMVKRPSIVILDEPTTAIDPAGVEDVLALIRELAHGGAAILLASHLLHQVQQVCDE
                     VGIFVAGKLIASGAMDKLSGQLATGAIEIEVAAPPPAEAVRDAAAKVNGVTSVEKDPR
                     DPKMWVVRAKRDVRADLARALVEAGHPPYHLRRRGDELDEIYRRYFADYATDTEGAA"
     gene            8725..9822
                     /locus_tag="VIF08_00275"
     CDS             8725..9822
                     /locus_tag="VIF08_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8134446.1"
                     /translation="MTGQSISLAATGATARGRKRKNIRAESAPPAQSAKERRYQRAAR
                     PAGIPAGWRVIAAKEFADHVSSIRFLVLTLVLTLAAAAAVYSAAGGLKALAPSASGAQ
                     SLFLYLFFYQPEGTNIPTFVYFTALLGPVLGIAFGFDGVNGERSEGTLPRLVSQPIHR
                     DDVINGKFVAGLAAIAVIFVAIIAVVGAIGVLQLGILPGIEEVVRLAVWVVLAVVYVG
                     LWLAVALLCSVVFRSAATSALVSIAAWLVLTLFVSLLVGVLADAVSPTGGESLPELIA
                     NESAKRNLSFISPSQLFTESTRALLDPSLRTFDVTAYLELRLEERALPSSLSLDQSLL
                     VVWPQFIGLIALTSGAFAVAYVSFMRQEVRA"
     gene            9839..10216
                     /locus_tag="VIF08_00280"
     CDS             9839..10216
                     /locus_tag="VIF08_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134447.1"
                     /translation="MKGDRVEAVVDTGQGTQTFEIVARRAGRRIEITNVRGVVEVTEV
                     TRAGTPIRTGRFMANRLIALVEHPAVEGNGSGVEVETRRRMNPESAPRARSRRSDSMT
                     DGPRQLTVLGDDDRNDDGGDGGA"
     gene            complement(10243..11406)
                     /locus_tag="VIF08_00285"
     CDS             complement(10243..11406)
                     /locus_tag="VIF08_00285"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8134448.1"
                     /translation="MTTPSPVRPADRATPPAVQPLRVALLSPPFLPVPPPGYAGTERV
                     VATLAQGLHERGHQVTLFAPGDSDVPYELVPSVPHSLWRNGHRGDASSYIEATVGLAW
                     EHATRFDLIHSHLDTAGFTMARHCGTPVLTTLHRRLDTTGAADMIDAFPDVALVAISD
                     SQRRWNPDANWIATIHHGLDFSSTPQSIDAGDYLLLVGRIAREKGIEEAIELAERTER
                     PLVIAAKVYDTREQRLFDAVVRPHLKKGLIDWRGEVEPAQRDELMAGALATLMLGAWP
                     EPFGLVAIESMATGTPVIARRAGANTETVQHGTSGFLVDDIDEAVLAVSRVGRLDRAK
                     IAGYARESFSAERMVAQYERAYAQVLAQAERAEAGDAAEPLHLVARAEDMAAG"
     gene            complement(11507..12607)
                     /gene="greA"
                     /locus_tag="VIF08_00290"
     CDS             complement(11507..12607)
                     /gene="greA"
                     /locus_tag="VIF08_00290"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8134449.1"
                     /translation="MPTQNTPELLRSLGLTVDGPARWGAPVPSRAPGIIVVELPGGAD
                     AAPLDLQALRRWLDRVPSLTLDGERPTPQVLQRRLAQDWLPGQPVLFVGRSTRSIGAR
                     LSAIYATTLGDAKPNSSGHWLKTLANPGELRVWWAETTAFEEYEDALLEALGVTPWAN
                     NSIAGTLRSEAESAAAAQRPTGGAAAAAAAAKVKARQREVVRKAAPPKLAPRPMPAKT
                     ALSKEGLERLTAELADLRDNARPQIIARVKAARELGDLRENGDYEYARKEQSFIEGRI
                     QALEQLIRSSVVIDGPVSLDVVHLGSTVDLEHEGERITYKIVGTAEAHPASGRLSDAS
                     PVGRALLGARVGDEVTVPLPGGAVQYRVVGVR"
     gene            12705..15464
                     /locus_tag="VIF08_00295"
     CDS             12705..15464
                     /locus_tag="VIF08_00295"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY8134450.1"
                     /translation="MTTIMREASTSLAPDQASDVLRASRHPLDPLFKPHTVAVIGATE
                     KKGSVGRTVLWNLISTPFGGTVLPVNPKRDNILGIRAYPSLADIHGPVDLAVIVTPAP
                     SVPDVMAECAAKGVKGAIVISAGFREIGPEGAELERRILAQAREARIRVIGPNCLGVM
                     NPVGGMNATFAAGMAAPGSVGFVSQSGALLTAVLDWSAREGVGFSSVVSLGSMLDVGW
                     GDVIYYMGDDPHTKAILIYMETIGDARAFLSAAREVALTKPIVVIKPGRTAEASKAAA
                     SHTGSLTGSDEVLEAAFRRVGVLRVERISDLFYIAEVLAKQPRPRGPNLTIVTNAGGP
                     GVLATDTLISGGGRLTKLSEAATAAYNEVLPSVWSHNNPVDIIGDAPPERYAKALEIA
                     AADEAADGMLVILTPQAMTDPTETARELVPYAQIKGKPVLASWMGGKDIDEGTAILRR
                     AGIPTFDYPDTAARMFNYLWQAQENVRLLYETPTLATDEAGNGLDRAAATEVIAAARR
                     DGRTLLTEVESKAVLAAYGIPITDTRVCATVDDAVAAAAAIGYPVVLKLYSHTISHKT
                     DVGGVQLNLADEADVGRAFAEIHDAVVAKRGAQHFEGVTVQPMINYTGYELIVGSSTD
                     PQFGPVLLFGMGGQLVELFKDRALGLPPLTTTLARRMIERTTISGAFAGIRGRDPIDT
                     TALEQLLVRFSMLVAEQRRIAEIDINPLLASPERLIALDARIVLHPAEIGDADLPRLA
                     IRPYPLQYVASWTARDGSRYLIRPIRPEDEAMLVKFHADLTEETVYSRYFEHLGLSQR
                     TAHERLTRVAFNDYDREMALVAEETDAQGERRIVAVGRLSKAHAVPEGEFAILVGDPW
                     QGKGLGTELLRRLVEIGRAEGLELIWADLLASNYGMRRTAQSVGFRLIDKPTETTTRA
                     ELRLN"
     gene            15567..16022
                     /locus_tag="VIF08_00300"
     CDS             15567..16022
                     /locus_tag="VIF08_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134451.1"
                     /translation="MLGHMDIRRLTVLGLLTLTCALLFVGLTNIQWCMPALHSALVFA
                     TALGTWWLAREDLALNKGGAGTRLVLTGVALGLGLTWVSAGTVAVIDVAQGAQWTSGE
                     FAQVVRRFIEDVARHWTPLGLVASFVGTALSLMLNEIGSSTSGRGQQTQ"
     gene            complement(16099..16797)
                     /locus_tag="VIF08_00305"
     CDS             complement(16099..16797)
                     /locus_tag="VIF08_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134452.1"
                     /translation="MIKRLQRLGLVLALFGLAFVAAGGYAFIKVQDGQKALTAFSAAQ
                     SVKLSYDEQGQLGGNSEESQGIMNLLTKDWGYAVDKAEFNPADPMVNTASEYMYQMAT
                     ISYHVLHGTQNITLDEDYTAADGTFYTAGTPIPFNVDGRYYSQFDRSDPVQAAARGLA
                     WSPTALALVGELGVGAVTASSLQMGLGVAATVGGLGFVFFIAGLGLVWAVRPETAKAR
                     VRVGQPALVPAAAE"
     gene            17081..>17314
                     /locus_tag="VIF08_00310"
     CDS             17081..>17314
                     /locus_tag="VIF08_00310"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEY8134453.1"
                     /translation="MAEDRKERVDRELIELLNELRVALPGVQVLFAFLLVVPFQQGFS
                     SVSEVARVAYFAGLASCAAASAFLIAPATYHRIN"
BASE COUNT         2668 a         6002 c         5962 g         2682 t
ORIGIN      
        1 ggcgtgcgca cgcccatgca gtggagcgcc gatcgcaacg ccggcttcag cgatgccaac
       61 cggcagcgtc tcttcctgcc ggtgatcacc gaccccgagt acagctacga ggcaatcaac
      121 gtcgacgccc agcaggccaa cccgagctcg ctgctgtggt ggatgaagcg cctaatcgcg
      181 ctgcgcaagc agtaccaggc cttcggtcgc ggttcgctga cgttcctcac gccggagaac
      241 cggcgcattg tggccttcgt gcgcgagttc ggcgacgagc ggatcctcgt ggtcgccaac
      301 atgtcgcgct tcgtccagca cgccgagctg gatctaacgc catggcgcgg tctcattccg
      361 gtcgagatgt tcggccgcgt cgagtttccg cggatcgagg aacgaccgct cttcctgacc
      421 ttgggcccgc actccttcat ctggttcgcg ctggaggcgg acccgagtgg acgcggccac
      481 gagatccggc cggtcgagga gaaggcaccg atcatcagcg ggccagccga cctcgagcgg
      541 ttgctctccg gcggcgcgcg ggcgctggcc agcgtgttgc cgggctacgt gcgcgcgcgg
      601 cgctggttcc gctccaaggc ccggcgcatc aagggcatcg cgctgcgcga tgcgctccgg
      661 gtgccgtttg acggctctgc agacatccag gcacggctgg cgatcttcga tgtcgagttc
      721 acggagggcg aagcggagac atacgtgctg cccctcgcgc tggcgcccgc cgagcgcgtc
      781 gaagccgcgc tcgctcccac gcctcaggcg atcgtcgtca ccgtcttgcc ggacggcgaa
      841 ggcggcgccg cgtggacgct ctatgacgcg atgtatgagc ccgcgttcgc gacggggctg
      901 ctcgatgcgg tgagtggcaa gcgccgcatc tccggccggc gcacggagct caccggatcg
      961 cctacgccgg cgctgcgccg gttgcgcggc gggcgcgggg cagcgttgac accggccgtg
     1021 ggccgggccg agcagagcaa cacatcggtc gtcttcggcg accggctgat cctcaagctg
     1081 ttccgttgcc tggagccggg cgcgaaccct gagatcgaga tcggccaggc attgactgac
     1141 gcggccttca gccacacgcc ggcactcggc ggctggttgt cgctgagcgg ccggcgcgag
     1201 ccgcaggcgt tgggcgtcgt acaggaatac gtggccaacg agggtgacgc gtgggcgctg
     1261 acgctggacg cggtcagcga cttctacgag cgcgccgcgg caaccgacgc cgagactgag
     1321 gcgcgcgccg agatcgtccc ggagatgacc ggtgactacc tgtatttcgc ccggctcctc
     1381 gggacgcgca ccgcggagct gcatcgcgtc ctggccgggc gcgacgattc cgcgttctcg
     1441 tccgagcctt tcagcgctct ctaccagcgc agcactttcc agaacctgcg caaccaggcg
     1501 agcgggacga tcgccctact gcgacgccgg ctcggcgacc tgcctgatga cgcgcggccg
     1561 gccgccgagc aggtgatcgc atcgtctgac gagatcctgc ggcggacgcg cgagctgacc
     1621 caggcgcgca tcggcgcggt gcgcatccgg acgcacggcg actaccacct gggccaggtg
     1681 ctatggaccg gcaaggacgt ggtgatcatc gatttcgagg gcgagcccgg ccggcccctg
     1741 tcggagcggc gccaccggcg ctcggcgctg gccgacgtcg cgggcatgtt gcgctcgttc
     1801 cactacgcgg cattcggcac gctgcttaat ccgcgcctcg gcggcgctgt gcggccggag
     1861 gacatcgcgc gcctcgagcc gtgggcgagg gcgtggtacg cgacggtcag cgccgcgttc
     1921 gtggacgcgt atctcgaagg agccgcggga cagccgttcg tgccgcgcgc ccgcgccgaa
     1981 caggttgcgc tgctcgacct ggcgatgctc cagaaggtcc tgtacgagct cacctacgag
     2041 ctcaacaatc ggcccgactg ggtgtcgatc ccgctgcgcg ggctgctcgc gctgctgggc
     2101 acggacgatg ggcacgtgcc gaccgcgccc gccgcgcccg ccgcgccaga agcggccgtc
     2161 ccggacgatg cctgaggcga tggcccagcc gcagcaagcg cggcgacaac ctgagcccca
     2221 ctggggtctg cgcagcgcgc tcgtgacgct gggtgtggcg gcactcgtca tgggcgtggc
     2281 cgtcgccctc aaggcactgc tcctgtccgt ttccaacgcc aacgctgggt tcatcgtcta
     2341 tatcccggcc gtcgcggtgg tggcctggta tcgcggtctg ctgggcggtg tgctcgcgac
     2401 actgctcggc gcgctcgctg actcggtgct cttcgtgccc gcgctcttcg tcgccgcggc
     2461 cgatctcagc agcgtgcagg tccggctgct ggcgtacctt gccggcggca ccgcggtgtc
     2521 gtacctgtcc tatcgactgc gtagcgagcg cgatcgggcg cgcctcgaag cgaccgagcg
     2581 gcgccacgcc ctgcagcgcg ccgcccagat gagcgacgag ctgggtcgca tcgcggccaa
     2641 cgagcgccgg gcaagcgagc tgcgtgacgc attcaactcg atcgtctcgc acgagctgcg
     2701 caccccgatc accgccatct acggcggcgc caagcttctc gcccggcgcg accggccgct
     2761 cgacgaggcc acacgccagg agctgatcga ggacctggaa ggggaggccg accggctgta
     2821 caggctggtc gaggacctgc tggtgctgtc gcgctcggag tacgagtcgc tggagcgctt
     2881 cgacgatccg gtcgcaatcg cgcacgtggt gcgccgcgtc acgctctccg agcaggtgcg
     2941 ctggcccggt gccaggttcg cgttcagcgc gaccaccacc atgaccgtgc gcggcgacga
     3001 gacatacgtc gagcaggtgc tgcgcaacct gctcagcaac gccgcgaagt acagcccggc
     3061 cggaaccacg gtcgaggtgg tcctggatga gacgcccgag ggtgcgcgtg tgcgcgtgct
     3121 cgattcaggg gcgggtgtgc gcaccgacga ggtcgatcgg ctgttccagc tctactaccg
     3181 ctccccggag accgcggcga aggccggtgg cgcgggcatt ggcctgtttg tgtgtcgcgc
     3241 tctcgtcgag gcgatgggcg gccgcatctg ggccgcgccg cggccggagg gtggctccga
     3301 gttcggcttc atccttcggc gctacgccga cgacgacgct ccccgtagcg aggcgaccta
     3361 gaagggcggc cagggtacgg ccggtcgcgc ggggtcgggc tccgggaagc gcccgtgctc
     3421 gagcagcgcg gcaacccgtt gcgcggtggc gcgtacctcg tcgcgcgtaa gcagctcagt
     3481 cagcgccgcg cccaggtcac ccgcgagagc cgcagcgacg cgacccagcg cggccgtctc
     3541 cgactcgtcg aacggctcgc cgcgccacgc ccacaggacc gtgcgcagct tcggctcgac
     3601 cgcgaagcag atgccatggt caacgccgcg caacgtgccg tcgggcagcg gcaggatgtg
     3661 gccgcccttg cgatcggcgt tattggccac gagatcgaac acgcacatgg gccgcaggcg
     3721 cgggtcgggc ccgactatca gctcgaccac gtcgaccccc ggatcggttt ccagccacgc
     3781 ctgtacggcg ccctcaccga atggcccgtc gcgcaacacc gttggtggca cgatcgccca
     3841 gcccgtcgcc tccgataaca ggaacgcggc cacctcgcgc cgccccaggg tgccgtccgg
     3901 gaagtcgtgc aacgggcgct cgccgcgcgt cggtttgtag acgcacagcg gcccgggcgg
     3961 cagctcgtgg tccggccact cgtcgggatc gaagagcgtg tcggcgccga tgcggcacag
     4021 gaagctcgcg ttggacgcgt cggtcaggcg gccgacgatg gtcaactcac cgtcgcgcag
     4081 caggtcgagc gacgcgattg atggcgcgga gtccgtgggg tccagcgcag atcctgcgcg
     4141 gctagttcag gagctcgttg gcgtggccgt tgcggcgcac gcagaagtgg ccggtcggat
     4201 cgagcggcgc gccgcacagc ggacatggcg gccggccggc gcgcacaacc tccagggcgc
     4261 cgctggcaaa gctcagcgcg gcttgaggct ccagctccac ccgaacgacg tcgcgtggat
     4321 cgtccatgtc gcccgccagc gagcgcagct cgatgccttc gtccgcggcg ccttcggcag
     4381 cctccatctc gtcctcgtcg gcgtcgtcgg catccgcttc ttcgtcgatg tcggcctcgt
     4441 cgatgtcggc ctcgccgatc tcgcgcgcct cgatgacgac ccggcccacc tcaccgtccc
     4501 agcccagagt catcgtgccc acgcggaatt gctcgacgac tggctcggcc agctctggcg
     4561 ctggccccgg caggtcgtcg ccgatggtga cgccgccctc gcgcagcgcg agcaacagcg
     4621 cggcaatccg ttcggccagc agcgcgacct gcgtcttctc gagcgcgacc gagatctgct
     4681 ggctgccctt gctcgcctgg atgaagaacg tccgctgacc gggcgtgccc agcgcgccgg
     4741 caatgaagcg gtctgggcgg tcaaacgaat agacgcgtcg cggcatggtg gcgctgactc
     4801 taatactctt caggcccgca ccacttcgac tgagaggttg atcccacttg cagttccgcg
     4861 tcgtttccag cctcgagggc gagcacctcg acgccctgat cgtgcccgtc ttcaaggaag
     4921 gtgacgcggc gagcaacacg cccgcggatg tccgcgacac ggccgcgtgg gtcgcccggg
     4981 agcacggcga caccaagatg ttcgcgccga tcacgcacct ccagagcagc gccgagagct
     5041 cgaccaggct catcgtcgtc cccgccggcc agcgcgccga cttcgacgtc gcgcgctcct
     5101 ggcagtttgc cagcgcgggt gtccgtggcc tgtggcagtc gacggccacg cgcatcgcga
     5161 tctgtctcga gtcggacgca ctcgacgccc aggaagcggt gcagggcgct gtcgagggcg
     5221 tgatctacgc gatgtggcgg ccggagagcc accgcacgac ggagaaggat cgccagctgc
     5281 cgccactcga ggaggtgctg ctggtgatcg gtggcacggc gagcgactac gaggcggcca
     5341 tcggccgcgg cacgttcgtc ggcgaggcgg tcaactggtc gcgcggcgtg gccaacgagc
     5401 cggccaacct gatgaccccg acccacatgg cgcaagcggc gcgcgagatg gcgtcgcaga
     5461 cgagcctcaa agtggaggtc ctgggcgagg acgaggcgcg cgatctgggc atgggcagct
     5521 acctctcggt tgcgcgcggc agtgatgagc cggcacaggt cgtcgtgctg cgccaccacg
     5581 gccgcccggg cgacggctac gacctcgccc tggtcggcaa gggcatcacc ttcgattcgg
     5641 gcggcatctc gatcaagccc gccgaggaca tgcacctgat gaagtacgac atgagcggcg
     5701 ccgcgtcggt gctcgcggcg gccggcgtgg tcgcgcgcat gaacctgcca ctcaacctga
     5761 tcgccgtcgc gatgtgcacg gagaacctgc ccggcggcca cgcgaccaag ccgggcgacg
     5821 tggtgacgag catgagcggc aagaccgtcg aggtcaccaa caccgacgcg gagggccgcc
     5881 tcgtgcttat cgacgggatc acttatgccc agcgcgaagg cgcgaagcgg gtggtcgacg
     5941 tggccacgct caccggcgcc gtgcgtatcg cgctgggccg gctgtacacc ggcgtcctcg
     6001 ggcggccgca gtcgttcatc gacgcggtgc tgggcgccgc tgaccggagt ggcgagcgca
     6061 tgtggccgct gccgctcgac gatgagcatc gcgaggagat gaagagcgag gtcgcggacc
     6121 tgcgcaatag ctcgggcggc cgcctgggtg gcaccatcct cggtgcggca ttcatcgacg
     6181 ccgcggtcga cgctgaaacg gaatgggcgc acctcgacat cgccagcact gcctggtacg
     6241 aagacgatcg gcccttctcg cccaagggcc cgcagggcgc ggccgtgcgg accatggtcg
     6301 agcttgcgac gaccatcgcc agggccggct gagggttttg ctccgcttac aggctgtgaa
     6361 tcggctggga gaacgcccag atcggtgaag aaaatgccac ccgatagcgg ctcgaccaaa
     6421 aatcgctgac tgagcgacaa tcgcggcgcg aacggctgcg agctgacgag aggactccgg
     6481 aaacgtcgcc cggcctgccg ccacggggcg ggttcgcgaa gtgaaaccaa tcgcatgagg
     6541 agttcgctga agatgcgcgt tctgcgggct ggtgccgccg cactgttgct cggactgacg
     6601 ctggcgccgg tggcgctggc ccagggtccg ctcgagctga ccacccagta cccggcggtc
     6661 accgccgacc cgggctcaac tgtcaagttc aacgtgatcg cgatcacgga cgtcgcaacg
     6721 cgcgtggacc tgtcgaccac cgctcccacg ggctggacag tcaggatgcg tggcggcgga
     6781 tcgaccgtca gcgcggtcaa caccgccccg ggccccatca ccgaaggctc gtccaccaac
     6841 cagatcgccg gtgctttcac cgttgagatc gatgtgccca tcgacgtcgc ccccggggtc
     6901 aaccacgtcg tcgtcaccgg ccgcaccgcg ggcggcgcga ccgcctcgct gtcgcttgac
     6961 ctgaccatcg aggcgcaggc cgcgggtgcc gtatcgctga ccacgaactt ccccaacctg
     7021 cgcgggtcga ccgcgaccgc gttcaggtac aacgtgcttc tcaacaacga caccaaccag
     7081 cagctgacct ttggccttga ggcccagggt ccggcgggct ggacggtcga ggcgaagccg
     7141 gcggccgagc agggcgctac caccacgacc gtcgatgcgg gcagcaactc gacgatccag
     7201 gtgacggccg atccgccagc ggacgccgtg gccggtatct acgacatcaa gatcatcgcc
     7261 cagggcggcc ccgagcccgt cgagctcgac ctgaccgtcg agatcaccgg ctcctactcg
     7321 ctcgggctga acacggccga tgagcggctc aacgcgaccg ccacggcggg atcgaagacc
     7381 acgctcgagc tcgttgtcac caacacgggc agcgccccgg tggccaacgt caggctcacg
     7441 gcgactcccc cccgcggctg gaagatcacc ttcgaccagg agaccatccc ggtaatccag
     7501 ccgggcgttg ataacggtgt gaaggtagtg gcaacgatcg agccgggggg caacgcggtc
     7561 gcgggcgatt acgtgctgac cttccgcgcc acatcgggcg aggagtcgac cgcgactgac
     7621 accatcgacg tgcgcacgac cgtcgaaacg tcacccatcg gcctggtcat cggcatcggc
     7681 atcctgatcg tcgtggcggc cggcctgttc ctcgtcttcc agcgctatgg ccgtcgctga
     7741 gccggccgcg cacacaactg ccggccagcc gcagccggta ataaagaccc gggcgctgac
     7801 caagcgctac ggcaacttca ccgcggtcga caagctcgat ctgtccgtgc gcccgggcga
     7861 ggtctttggc ctcctcgggc ccaacggcgc gggcaagacg acaacgatcc tgatgctgct
     7921 cggcctgtcc gagccgacat cgggctcggc acgcgtcctg gacatggatc cggcgcgcga
     7981 tccgatcgcg gtcaagcgcc gggtgggcta cctgcccgac gcggtcggct tctacggcgc
     8041 catgaccggc cgccagaacc tgcaatacac ggcgcgcctc aacggcatcc ccaggaaggc
     8101 cgccgacgat cacatcgacg agctgctcga gcgtgtcggc ttgacgaatg ccgccaactc
     8161 caaggtcgaa acctattcgc gcggcatgaa gcagcgcctg ggtctcgccg acgtcatggt
     8221 caagcgcccc tcgatcgtga tcctcgacga gccgaccacg gccattgacc cagccggcgt
     8281 cgaagacgtt ctggcgctca ttcgtgagct ggcgcacggt ggcgccgcga tcctgctggc
     8341 gagccacctg ctgcaccagg tgcagcaggt gtgcgacgag gtcggcatct tcgttgccgg
     8401 caagctcatc gcgtcgggcg cgatggacaa gctgtcaggc cagctcgcga cgggcgcgat
     8461 cgagatcgag gtcgcggcac caccgcccgc agaggcagtc agggacgcag ccgcgaaggt
     8521 caacggcgtc acgtcggtcg agaaggatcc gcgcgatccc aagatgtggg tcgtgcgcgc
     8581 caagcgcgac gtccgggccg acctggcgcg ggccctggtc gaggccggcc atccgccgta
     8641 ccacctgcgc cgccgcggcg acgagctcga cgagatctat cggcgctact tcgccgacta
     8701 cgccacggac acggagggcg cggcatgacc ggccagtcca tctcgctcgc cgctacgggc
     8761 gcgaccgcgc gcggccgcaa gcgcaagaac attcgcgctg aatccgcccc gcccgcgcag
     8821 tccgccaagg agcgccgcta ccagcgcgcc gcgcggccgg caggtatccc agcgggctgg
     8881 cgcgtcatcg cggcgaagga gttcgccgac cacgtatcga gcattcggtt cctggtgctg
     8941 acgctcgtcc tgacgctcgc cgcggcggcc gcggtgtact cggctgctgg cggactaaag
     9001 gcgctggccc ccagcgccag cggcgcccag tcgctgttcc tctacctgtt cttctaccag
     9061 cccgaaggca ccaacattcc gacgttcgtg tacttcaccg cgctccttgg accggtgctg
     9121 ggcatcgcat tcggcttcga cggcgtcaat ggcgaacgct cggagggaac gctgccccgg
     9181 ctcgtttccc agcccatcca ccgcgacgac gtcatcaacg gcaagttcgt cgccggcctg
     9241 gcggcaatcg cggtcatctt cgtcgccatc atcgccgtcg tcggggccat cggcgtgctc
     9301 cagctgggca tcctgcccgg catcgaggag gtcgtgcgcc tcgcagtgtg ggtcgtgctg
     9361 gccgtggtct acgttggcct atggctggct gtcgcgctgc tgtgctcggt cgtcttcagg
     9421 agcgccgcga catcggcgct ggtgtcgatc gccgcatggc tcgtactgac gctgttcgtc
     9481 agcctgctgg tgggcgtcct tgctgacgcc gtctcgccca cgggcggaga atcgttgccg
     9541 gagctcatcg ccaacgagtc cgcgaagcgc aacctgtcgt tcatatcacc cagccagctc
     9601 ttcaccgaat cgacgcgagc gttgctcgac ccgagcctgc gcacgtttga tgtgaccgcc
     9661 tatctcgagc tcaggctgga ggagcgcgcg ctgccgtcga gcctgtcgct cgaccagagc
     9721 ctgctcgtgg tctggccgca gttcatcggc ctgatcgcgc tcacgagcgg cgccttcgcg
     9781 gtggcctacg tgtcgttcat gcgccaggaa gtgcgcgcct aaggcgtatc ctccggccat
     9841 gaagggtgat cgggtagagg cggtagtcga taccggccag ggcacccaga cgttcgagat
     9901 cgtggcgcgg cgggccggcc ggcggatcga gatcaccaac gtccgcggcg tggtcgaggt
     9961 cacggaggtt acgcgggccg gcacgcccat tcggaccggc cgattcatgg ccaaccgact
    10021 catcgccctg gtcgagcacc cggcggttga gggcaacggc tccggcgtcg aggtagagac
    10081 gcgccgccgg atgaacccgg aatcggcgcc gcgcgcccga tcgcgtcgct cagattcgat
    10141 gaccgatggg ccgcgtcagc tgaccgtgct gggtgacgac gaccgcaacg acgatggcgg
    10201 tgacggcggg gcctgattcg gcgcgttagg ggcggctgag actcagcctg cggccatgtc
    10261 ttccgcgcga gccacgaggt ggagcggctc ggctgcatca cctgcctcag cccgctcggc
    10321 ctgcgccaac acctgcgcgt aagcccgctc gtactgcgcg accatgcgct ctgccgagaa
    10381 gctttcgcgc gcgtagcccg cgatcttggc gcggtcgagt ctgcccacgc ggctcaccgc
    10441 cagcacggct tcgtcgatgt cgtcgaccag gaatccgctc gttccgtgct gaacggtctc
    10501 cgtattggcg cccgctcggc gggcgatgac cggcgtgccg gtcgccattg actcgatcgc
    10561 cacgaggccg aacggctcgg gccaggcgcc gagcatcagt gtcgccaggg cgccggccat
    10621 gagttcgtcg cgctgcgcgg gctcgacctc gccgcgccag tcaataaggc ccttcttgag
    10681 gtgcggtcgc acgacggcgt caaacagacg ctgctcgcgc gtgtcgtaca ccttggccgc
    10741 gatgaccagc ggccgctcgg ttcgctcggc caattcgatc gcctcctcga tgcctttctc
    10801 gcgggcgatc cggccaacga ggagcaggta gtcgccggca tcgatcgact gaggcgtgga
    10861 actgaaatcg agcccgtggt ggatggtcgc aatccagttg gcatccggat tccagcgccg
    10921 ctggctgtcg ctgatcgcga ctagcgcaac gtcaggaaag gcgtcgatca tgtcggccgc
    10981 gccggtggtg tcgagccgcc tgtggagcgt cgtcaggacc ggggttccgc agtgccgcgc
    11041 catcgtgaac ccggccgtgt cgagatgtga gtggatcagg tcgaagcgcg tcgcgtgttc
    11101 ccaggcgagg ccgaccgtcg cctcgatata cgacgacgcg tcgccacggt ggccgttgcg
    11161 ccagagcgag tgtggcacgc tcgggacaag ctcgtacggc acatcggagt cgcccggcgc
    11221 gaagagcgtc acctgatggc cgcgttcgtg caggccctgg gcaagcgtcg ccacgacgcg
    11281 ttctgtgccg gcgtagcccg gcggtggcac aggtaggaaa ggcggcgaca gcagcgcgac
    11341 gcggagcggt tgaaccgcgg gcggcgtggc acgatcagcc ggccggacgg gtgagggcgt
    11401 ggtcattggt ctcagcatgg ccgatctggc gtcgctttcc agtccctgcg caaggggcga
    11461 gcatcaaagt cagatagcaa agcgccatcc agatgcacgt aacggactag cggaccccga
    11521 ctacccggta ttggactgcg ccgcccggca gcggaaccgt cacttcgtcg cccacgcgcg
    11581 caccaagcag ggcccgtccg acgggtgacg cgtcggagag cctgcccgac gccggatggg
    11641 cctccgcggt gccgacgatc ttgtaggtga tgcgctcacc ttcgtgctct agatcgaccg
    11701 tcgagcccag gtgcacgaca tcgagcgaga ccgggccgtc gatcacgacg ctcgagcgga
    11761 tcagctgctc gagcgcctgg atgcggccct cgatgaagga ttgctccttg cgcgcgtact
    11821 cgtagtcgcc gttctcgcgc aggtcgccca attcgcgcgc cgccttgacg cgggcgatga
    11881 tctgcggccg cgcgttgtca cgcaggtcgg ccagctccgc ggtgagccgt tcgaggcctt
    11941 ccttcgacag cgcggttttg gccggcatcg gccgcggcgc cagtttgggc ggcgcggcct
    12001 tgcgaacgac ctcgcgttgc cgggctttca ccttcgccgc agccgcagcc gcagccgcac
    12061 cccccgtcgg ccgctgagcg gctgcggccg actcggcctc gctgcgcaac gtgccggcga
    12121 tcgagttgtt ggcccagggc gtcacgccca gcgcttcgag cagtgcgtcc tcgtactcct
    12181 cgaaagcggt tgtctccgcc caccagacgc gcagctcgcc cgggttggca agggtcttga
    12241 gccagtgtcc cgagctgttc ggcttggcgt cgcccagggt ggtggcgtag atcgccgaca
    12301 gccgcgcgcc gatggagcgg gtcgagcggc ccacgaagag caccggctgc ccgggcagcc
    12361 aatcctgcgc gagccggcgc tggagcacct ggggcgtggg ccgctccccg tcgagcgtca
    12421 gtgaggggac gcggtcgagc cagcggcgca gcgcctgcag gtccagcggc gcggcatcgg
    12481 cgccaccggg cagctccacg acgatgatgc cgggcgcacg gctggggacc ggcgctcccc
    12541 agcgagccgg gccgtcgacc gtgagaccca gggagcggag cagctcgggc gtgttctgtg
    12601 tgggcatccg cgcattgtcc ctgtttacgg tcaggccgta agtcacgcca cggactgact
    12661 catggcgagc gcggccgacc ccctcgcgcc gccatgctgc gactatgacc accatcatgc
    12721 gtgaagcgtc gacctcgctc gcacccgacc aggcgtcaga tgtcctgcgc gcgagccgtc
    12781 acccgctcga tccgctcttc aagccgcaca cggtcgcggt catcggcgcg acggagaaga
    12841 agggcagcgt tggccggacg gtgctgtgga acctgatcag cacgccgttc ggcggaaccg
    12901 tgctgcccgt caaccccaag cgcgacaaca tcctgggcat acgcgcttac cccagcctgg
    12961 ccgacatcca tggcccggtg gatctcgcgg tcatcgtcac ccccgcgccc agcgtgccgg
    13021 acgtcatggc cgagtgtgcc gccaagggcg tgaagggcgc gatcgtgatc tcagccggtt
    13081 tccgcgagat cggcccggaa ggcgcggagc tcgagcggcg catcctggcc caggcgcgcg
    13141 aggcccgaat ccgggtgatc ggccccaact gcctgggcgt catgaatccg gtcggcggca
    13201 tgaatgccac cttcgcggcg ggcatggctg cgcccggatc ggtcggcttc gtcagccagt
    13261 cgggcgccct tctgactgcc gtgctcgact ggagcgcccg cgagggcgtg ggcttctcgt
    13321 ccgttgtgag cctgggatcg atgctcgatg tgggctgggg cgacgtcatc tactacatgg
    13381 gcgacgaccc gcacaccaag gcgatcctca tctacatgga gacgatcggc gacgcgcgcg
    13441 ccttcctgtc tgccgcgcgc gaggtcgcgc tgaccaagcc gatcgtcgtc atcaagcccg
    13501 ggcggaccgc ggaagcatcc aaggccgcgg ccagccacac cggctcgttg accggctccg
    13561 atgaagtcct ggaggccgcg ttccggcgcg tgggcgtgct ccgcgttgag cgcatcagcg
    13621 acctcttcta catcgccgag gtgctcgcca agcagccccg gccgcgcggc cccaacctga
    13681 ccatcgtcac caacgccggc ggtccgggcg tgctcgcgac ggacaccttg atcagcggcg
    13741 gcggccgtct gacgaagctc tcagaggcgg cgacggccgc ctacaacgag gtcctgccca
    13801 gcgtctggag ccacaacaac ccggtcgaca tcatcggcga cgcgccgcct gagcgttacg
    13861 ccaaggcgct cgagatcgcc gcggccgacg aagccgcgga cggcatgctc gtcatcctga
    13921 ccccgcaggc gatgaccgat ccgactgaga cggcgcgcga gctggtgcct tacgcccaga
    13981 tcaagggcaa gcccgtgctc gcgtcttgga tgggcggcaa ggacatcgac gagggcaccg
    14041 cgatcctgcg ccgcgccggg atcccgacgt tcgactatcc cgacaccgcg gcgcggatgt
    14101 tcaactacct gtggcaggca caggagaacg tgcgtctgtt gtacgagacg cccacactgg
    14161 cgaccgacga ggcgggcaac gggctcgatc gtgccgccgc gacggaggtc atcgccgccg
    14221 cccggcgcga tggccgcaca ttgctcaccg aggtcgagtc gaaggcagtc ctcgcggcat
    14281 acggcatccc gatcacggac acccgggtgt gcgcgaccgt tgacgacgcc gtcgcggccg
    14341 cggccgccat tggctaccca gtcgtgctca agctgtacag ccacacgatc tcgcacaaga
    14401 cggacgttgg cggtgtccag ctcaacctcg ccgacgaggc cgacgtgggc cgcgcgtttg
    14461 ccgagatcca cgacgccgtg gtggccaagc gaggcgcgca gcacttcgag ggtgtgaccg
    14521 tccagccgat gatcaactac accggctacg agctcatcgt gggctcctcg acggacccgc
    14581 aattcggccc ggtgctgctc ttcggcatgg gcggccagct ggtcgagctc ttcaaggacc
    14641 gcgcgctggg actgccgccg ctgacgacga cgctcgcgcg gcggatgatc gagcgcacga
    14701 cgatcagcgg cgcgtttgcg ggcatccgcg gccgcgatcc gatcgacacc accgccctgg
    14761 agcagctgct ggtgcggttc tcgatgctcg tcgcggagca gcggcgcatc gccgagatcg
    14821 acatcaaccc gctgctcgca tcgcccgagc ggctgatcgc gctcgatgca cgcatcgtgc
    14881 tccaccccgc ggagatcggt gacgcggatc tgccgcgcct ggcgatccgg ccctatccgc
    14941 tgcagtacgt ggccagctgg acggcgcgcg acggatcgcg ctacctgatc cgaccgatcc
    15001 gcccggagga cgaggccatg ctggtcaagt tccacgccga cctgaccgaa gagaccgtgt
    15061 actcgcgcta cttcgagcac ctgggcctgt cgcagcgcac tgcgcacgaa cgcctgacgc
    15121 gcgtcgcgtt caacgactac gaccgcgaga tggctctcgt cgccgaagaa acggacgcgc
    15181 agggggagcg gcgcatcgtc gccgtcggcc gcctgtccaa ggcgcacgcc gtgccggagg
    15241 gcgagttcgc gatcctggtc ggcgatccgt ggcagggcaa gggactgggc acggagctgc
    15301 tgcgccgcct cgtggagatc ggccgcgctg aaggcctgga gctgatctgg gccgacctgc
    15361 tcgcgtccaa ctacggcatg cgccggaccg cccaatcggt gggcttcagg ctcatcgata
    15421 agccaacaga gacgaccacc cgcgccgagc tgcgcctgaa ctagcagggg gctattcctg
    15481 tcgaatggct ggcatcaggc ggccataggg cacgtctcgg gcgcccacgc aggtttctac
    15541 acttgccggc cggaggtgga gttcgggtgc tcggccacat ggacattcgc cggctgacgg
    15601 tccttggctt gctgacgctg acgtgtgccc tcctgtttgt tggcctgacc aacatccagt
    15661 ggtgcatgcc ggcgctccac tcggcgctcg tgtttgccac ggcgctcggc acgtggtggc
    15721 tggcgcgcga ggacctggcg cttaacaagg gcggcgccgg gacccgcctg gtgctcactg
    15781 gcgtggcact tggcctgggc ctgacgtggg tcagcgcggg gaccgtcgcg gtcattgatg
    15841 tcgcccaggg cgcgcaatgg acgagcggcg agttcgctca agtcgtgcgt cgcttcatcg
    15901 aggacgtggc gcggcattgg acgcccctgg gactcgttgc gtcgtttgtc ggaacggccc
    15961 tgtcgttgat gctcaacgag atcggcagca gcacatcggg gcggggtcag cagacacagt
    16021 aaaagagccg ccgggcgtcc tgggggaccg cccggcggga ggaggtgtga ggtcaggggc
    16081 gcccggcgcc ccgggctgtc attccgcggc tgcgggcacc aatgcgggct ggccgacccg
    16141 gaccctggcc ttggccgtct ccgggcggac cgcccagacc aggccgagac cggcgatgaa
    16201 gaaaacgaac ccgaggccac ccaccgtggc cgccacgccc agccccatct ggagcgagga
    16261 ggctgtcaca gcacccacac ccagctcgcc cacgagggcc agcgcggtgg ggctccaggc
    16321 cagaccgcgg gccgcggcct gaaccgggtc gctgcggtcg aactggctgt agtagcgccc
    16381 gtcaacgttg aacgggatcg gggtgccagc ggtgtagaac gtgccgtcag cggccgtgta
    16441 gtcctcgtcg agggtgatgt tctgcgtgcc gtgcagcacg tggtacgaga tggtggccat
    16501 ctggtacatg tactcagacg ccgtgttgac catcgggtcg gccgggttga actcggcctt
    16561 gtcgaccgcg taaccccaat ccttcgtcag caggttcatg atgccctgcg actcttctga
    16621 gttgccgccg agctgcccct gctcgtcgta gctgagcttg acgctctgcg ccgcgctgaa
    16681 cgcggtaaga gccttctggc cgtcctggac cttgatgaag gcgtagccgc ccgcagcgac
    16741 gaacgccaga ccgaacagtg ccaggaccag acccagcctc tgcagtcgct tgatcatttc
    16801 gagttctccc ttcgcagcta ggaccttcga gcttccttgc tcgttttcaa tggcctgact
    16861 gaaccttcgc tccacacggg cagccggcgc aggagaatta cggacctaat gcgtgtttca
    16921 tttgccgaag atggggggcc gaatggcacg tcccctgccg tgaccagcga caggggtgcg
    16981 cccggccgca ggacgccatt cgctcacggc gccgcgtgtc gcggctgcgc ctaggcggct
    17041 gcgcctaggc ggctgcgcct gggcggctag gattccacgc gtggccgaag accggaagga
    17101 gcgcgtcgac cgcgagctga tcgagctgct caacgagctg cgcgtcgcgc tgccgggagt
    17161 acaggtgctc tttgcattcc tgctggtcgt gcccttccag cagggcttct cgtcggtcag
    17221 cgaagtcgcg cgcgtggcct atttcgccgg gctcgcgtcc tgcgcggcgg cgagcgcctt
    17281 cctcatcgcg ccggccacgt accaccgcat caac
//
LOCUS       DATBRX010000006         3803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1678817, whole genome shotgun sequence.
ACCESSION   DATBRX010000006 DATBRX010000000
VERSION     DATBRX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678817"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VIF08_00315"
     CDS             complement(<1..446)
                     /locus_tag="VIF08_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY8134454.1"
                     /translation="MAEVGQVLAGRYKLVELLGQGGMATIFRGHDTQLGRDVAVKVLR
                     AEYGRDPAFVARFRQEAQSAAALSHPNVVQVFDYGMEAGDPFIVMEMIDGGDLAVVLK
                     ERGAFEPLAAARIAQQIFEALDAAHGRGIVHRDIKPTNVLLTTAGRV"
     gene            complement(513..1391)
                     /gene="galU"
                     /locus_tag="VIF08_00320"
     CDS             complement(513..1391)
                     /gene="galU"
                     /locus_tag="VIF08_00320"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY8134455.1"
                     /translation="MRVRKAVFPAAGWGTRFLPATKAQPKEMLPLVDKPVIQYGVEEA
                     VAAGIEQVIIVTSSQKRAIEDHFDHSYELEHLLEAKGDIDMLRRVRQIGDMVQISYVR
                     QKEQLGLGHAVLMAKELVGHEPFAVILSDDVVVGDRPCIGQLMDAYNTTHASVVAVME
                     VPHDDTSRYGIINPDLDADTNDEWLTKLKGVVEKPQPADAQSNLAVIGRYVLTPKVFE
                     KLEQTQRGAGGEIQLTDAINALTAEQDVYAYAFEGKRYDAGTTMGWLKASIELALERP
                     DLSAEFRRYLAGLDLT"
     gene            complement(1422..2354)
                     /locus_tag="VIF08_00325"
     CDS             complement(1422..2354)
                     /locus_tag="VIF08_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8134456.1"
                     /translation="MLMVATAAALFGTLSYITRGAEQVGMGAFPLVWWRGLVGTAVML
                     LVSGLFTLRAHRTGVIGAAWLPRANGRRLLIASLLGAAVNIAMFAAFVKTTIAVVLIA
                     FYTFPALVTLLAVPLYGERLTRVRLGALALSAAGLVLVVLSPLLSDTAVLLDPVGIAL
                     ALGAAVCQAGFILMAGRGFAPLSPARVATYAIFAAAAVALALALVAGDIAGLTVPLRE
                     PQAWIWILLGGITGAAIPTTAFVTGIGLIGPSRAAILMTLEPLVAITLAALLLKEQPS
                     PLQFLGGVAVLAAAVILQLVPSRVAPEPEFGRLV"
     gene            complement(2423..3454)
                     /locus_tag="VIF08_00330"
     CDS             complement(2423..3454)
                     /locus_tag="VIF08_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134457.1"
                     /translation="MSLNVLLPAVGAVLALIFAVALFDQWRERRRGFQLIWAVGMVFF
                     AIADACEAIGAIGGWSELLYRTWYLTGAVFTAAWLGLGTAFLLGKTRFGYAYALLVFF
                     GGFLAFVTRNTYGAGTIGLLALIAAAVVAIAIAIETYFQNERWPQIAAAAVVVASAIG
                     AYFAFTDPLSAPLPAQDAVPTGAAFTSPVRIFSLFLNVPGGIGLVLGAFFSAYVFMPK
                     KRYLPYSLDPGQKGDQFLFNLIIAVVAIPVNFVLSLPAAIVALLTGRIHSRVPATLLI
                     ALGGLIVSSGDSLARMGSTSMYSLAKVVGLVLIFLGFLVSIEAFREIRVPFTGRVLRS
                     ARNEHSAAP"
     gene            complement(3480..>3803)
                     /locus_tag="VIF08_00335"
     CDS             complement(3480..>3803)
                     /locus_tag="VIF08_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134458.1"
                     /translation="VFDENNSIRCAGCGEPIEGTPFRITILDIVAPEAPPSWAVGAAL
                     NPGPHQFHAQPDHFRSWARKRGFLFCRLSDVREIMRPVPIPGDAPRWGLCDGLHRQSH
                     EFVPA"
BASE COUNT          695 a         1245 c         1325 g          538 t
ORIGIN      
        1 acccggccgg ccgtcgtcag cagcacgttg gtcggcttga tgtcgcgatg gacgatgccc
       61 cgaccatggg ccgcgtcgag cgcctcaaag atctgctgcg cgatgcgcgc cgcggcgagc
      121 ggctcgaacg cgccgcgctc cttgagcacg accgcgaggt cgccgccgtc gatcatctcc
      181 atgacgatga acgggtcgcc cgcttccatg ccgtagtcga agacctgcac cacgttcggg
      241 tgcgacagcg cggcggcgga ctgcgcctcc tggcggaacc gggcgacgaa cgccgggtcg
      301 cggccgtact cggcgcgcag caccttgacg gcgacatcgc gcccaagctg cgtgtcgtgg
      361 ccgcggaaga tggtcgccat gccgccctgc ccgagcagct cgaccagctt gtagcggccg
      421 gcgagcacct gtcctacttc ggccaagaac tgcgctccaa atcggccaaa cccaaatgag
      481 gcaaaacgtt taccccttga tgcgtgacgt cctcaggtga gatcgagccc ggccagatag
      541 cgccggaact ccgcactgag atcaggccgc tcgagcgcga gctcgatcga ggccttgagc
      601 cagcccatgg tagtgcctgc gtcgtagcgc ttgccctcga aggcatatgc gtacacgtcc
      661 tgctcggcgg tcagggcatt gatcgcgtcg gtcagctgga tctcgccgcc ggcgccgcgc
      721 tgcgtctgct cgagcttctc gaacaccttg ggtgtgagca cgtaccggcc gatcaccgcg
      781 aggttgctct gcgcatcggc tggctggggc ttctccacaa cccccttgag cttggtcagc
      841 cactcgtcgt tggtatctgc gtcgaggtcg ggattgatga tgccgtagcg gctcgtgtca
      901 tcgtgcggca cctccatgac cgccacgaca gaggcatgcg tggtgttgta cgcatccatc
      961 agctggccaa tgcacggccg gtcgcccacg accacgtcgt cggacaggat cacggcgaat
     1021 ggctcgtggc cgaccagctc cttggccatg aggaccgcgt ggcccaagcc gagctgctcc
     1081 ttctgacgga cgtagctgat ctggaccatg tcgcctatct ggcggacacg gcgcagcatg
     1141 tcgatgtcgc ccttcgcctc gagcaggtgc tccagctcgt acgagtggtc gaagtgatcc
     1201 tcgatcgcac gcttctggct gctggtgacg atgatcacct gctcgatgcc ggcggccacg
     1261 gcctcctcga cgccgtactg gatgaccggc ttgtcgacca gcggcagcat ctccttgggc
     1321 tgcgccttgg tcgcaggcag gaagcgggta ccccaaccgg cggccgggaa gacagctttt
     1381 ctgacgcgca aaccgaacct ccgtgggtgg tgcgggcgat gctagaccag ccggccgaac
     1441 tccggctccg gcgccacgcg gctgggcacc aactgcagga tgaccgcggc cgcgagcacc
     1501 gccacgccgc ccaggaactg cagtggcgag ggctgctctt tcagcagcag cgcggcgagc
     1561 gtgatggcga cgagtggctc cagagtcatc aggatggccg cgcggctggg cccgatcagc
     1621 ccgatgccgg tgacgaacgc ggtcgtgggg atggccgccc cggtgatgcc gcccagaagg
     1681 atccagatcc atgcctgcgg ctcgcgcagc ggcacggtca agccagcgat gtcaccggca
     1741 accagcgcca gcgcgagggc caccgccgcc gcggcaaaga tggcatacgt cgcgacgcgc
     1801 gccggggaga gtggcgcgaa gccgcgtccg gccatgagga tgaagccggc ctggcagacg
     1861 gcggcgccca gcgccagggc gatgcccacc gggtccagca acacggccgt atccgagagc
     1921 aggggcgaga gcacgaccag cacgaggcca gctgctgaca gggcgagggc gccaaggcgt
     1981 acgcgcgtca ggcgctcccc gtagagcggc accgcgagca gggtcacgag cgccgggaag
     2041 gtgtagaagg cgatgagcac gacggcgatc gtggtcttca cgaatgccgc gaacatggcg
     2101 atgttcaccg ccgccccgag gagtgacgcg atcaggagcc gccggccgtt ggcgcgcggc
     2161 agccacgcgg cgccgatcac gccggttcga tgggcgcgca gcgtgaagag gccgctgacc
     2221 aggagcatca ccgcggtgcc cacgaggcca cgccaccaga caaggggaaa cgcacccatg
     2281 cccacctgct cggcaccgcg ggtgatgtag ctgagcgtcc cgaacaacgc cgccgctgtc
     2341 gcgaccatga gcatgccgag aagggtgttg ctcagggcag caggctcgct atcgggactt
     2401 tgggtgtcgg agtggagtca ggtcaggggg ccgccgagtg ctcgttccgc gcgctgcgca
     2461 gcacgcgtcc ggtgaacggc acccggatct cgcggaatgc ctcgatcgag accaggaagc
     2521 ccaggaagat caggacgagg ccgacgacct tcgcgagcga gtacatcgat gtcgagccca
     2581 tgcgggccag gctgtcgccg ctgctgacga tgagtccgcc gagcgcgatg agcagcgtcg
     2641 cgggcacccg actatgaatg cgcccggtaa ggagcgcgac gatcgcggcc ggcagcgaca
     2701 ggacgaagtt caccgggatc gcgaccacgg cgatgatgag gttgaacagg aactggtcac
     2761 ccttctggcc ggggtcgagc gagtacggca ggtagcgctt cttgggcatg aacacgtacg
     2821 ccgagaagaa cgcgccgaga acgaggccaa tcccgccggg cacattcagg aagagcgaga
     2881 agatccgcac cggacttgtg aaggccgcgc cggttggcac ggcgtcctgg gccggcagcg
     2941 gagcgctcag tggatctgtg aacgcgaagt acgcgccgat ggcgctcgcg acaacgactg
     3001 cggcggcagc gatctggggc cagcgctcgt tctgaaagta cgtctcgatc gcgatcgcga
     3061 tggccaccac cgcggcggca atgagtgcca gcaaaccgat cgtgcccgcg ccatacgtgt
     3121 tgcgggtgac aaaagccagg aacccgccaa agaaaacgag cagcgcgtac gcgtaaccaa
     3181 agcgcgtctt gcccaatagg aaggcagtgc ccagacccag ccacgcggcg gtaaagacgg
     3241 cgccagtcag gtaccacgtg cggtagagca gctcgctcca gcccccgatc gctccgatgg
     3301 cctcgcacgc gtcggcgatg gcgaagaaga ccatgccgac cgcccagatc agctggaagc
     3361 cgcgccggcg ctcacgccat tggtcgaaca gggctacggc gaagatgagg gcgaggacgg
     3421 caccgactgc cggcaggagg acgttcaggc tcattcagaa acctctggcc ttgggccggc
     3481 taggcgggca cgaactcatg ggattggcga tgcaggccgt cgcacaggcc ccaccgcggc
     3541 gcgtcgccgg ggatgggcac gggacgcatg atctcgcgca cgtccgacaa ccggcagaac
     3601 aggaagccgc gcttgcgcgc ccacgaccgg aagtggtccg gctgggcgtg gaactgatgc
     3661 gggccggggt tgagcgcggc gccgacggcc catgagggag gcgcttccgg cgcgacgatg
     3721 tcgaggatcg tgatgcggaa tggcgtgccc tcgatgggct cgccacagcc ggcgcagcgg
     3781 atcgagttgt tctcgtcgaa gac
//
LOCUS       DATBRX010000007         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3019885, whole genome shotgun sequence.
ACCESSION   DATBRX010000007 DATBRX010000000
VERSION     DATBRX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3019885"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VIF08_00340"
     CDS             <1..274
                     /locus_tag="VIF08_00340"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8134459.1"
                     /translation="RIHAIVTATAPDLTPKTYYGMPAWATPGKDGKVICFFQPASKFK
                     VRYCTLGFQPDAKLDEGNMWPTSWALTKLTAADEKKIAAMVRKAVG"
     gene            267..2693
                     /locus_tag="VIF08_00345"
     CDS             267..2693
                     /locus_tag="VIF08_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8134460.1"
                     /translation="MASVPAPDWGAIRVRGARENNLRDVSLDIPKRRFTVFTGVSGSG
                     KSSLVFETIAAESKRLIDETYSAFIQGFMPTLARPDVDSLEGLTTAIIVDQERLGANA
                     RSTVGTVTDANALLRVLFSRLAKPHIGGPQAFSFNIPSVHASGTITVERGGKAVRPPE
                     DGERRHYGGSVTVGGGTGRTEVRSFSLQGGMCPRCEGMGSVSDIDLAELFDERKSLAG
                     GALKIPGYTADGWAVRIFGGVVPLDVPINKLTAEQRQEFLYGEPRKVRLEGINLTYEG
                     LVPKIQKSMLSKDVGALQPHIRAFVERAVAFATCPECGGTRLSEPARKAKINGVSIAD
                     ACAMQISDLAAWLRDLDEPSVKPLLANLQHLFDGFVEIGLGYLSLDRASGTLSGGEAQ
                     RTRMIRHLGSSLTDVTYVFDEPTIGLHAHDVERTNALLHRLRDKGNTVLVVEHDPEVI
                     RSADHVVDMGPGAGPHGGTVVYEGPVAGLAGSGTATGRHLDARQEIKAAPRAPNGAIA
                     IRHARLHNLKDVSVHVPLGVLVAITGVAGSGKSSLIHGCMPRTDPSVTVIDQSAIKGS
                     RRSNPATYTGILDPIRKAFAKANGVKESLFSANSEGACPACNGLGRIYTDLGFMAEVA
                     SVCEVCEGRRFTDDVLHHTLRGKNIVEVLALPVEQARDFFTEKPVTQVLDRLDAVGLG
                     YVALGQELNTLSGGERQRLKLAIEMAADTQVYVLDEPTTGLHMQDVEKLIALLDRLVD
                     SRRTVIVIEHNLDVVARADWVIDMGPGAGHDGGRVVFEGAPADLARAKGSLTGQFLAR
                     HRAGTVAAGR"
     gene            complement(2751..2945)
                     /locus_tag="VIF08_00350"
     CDS             complement(2751..2945)
                     /locus_tag="VIF08_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134461.1"
                     /translation="MGVVHAKGFEMLAYFLSLLDRDERGQGLAEYALILALIAVLAIA
                     AVTFLGGSINAILSDTGNKI"
     gene            complement(3022..3291)
                     /locus_tag="VIF08_00355"
     CDS             complement(3022..3291)
                     /locus_tag="VIF08_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134462.1"
                     /translation="MERGESTIEFSLRTGDGPVLSVRMRRLSLGWVADVAGVGAGPGV
                     GRTARQALTAALLPLGDEQTRTLLADLGLLEPSVAVLVVERAQTA"
     gene            3448..>3642
                     /locus_tag="VIF08_00360"
     CDS             3448..>3642
                     /locus_tag="VIF08_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134463.1"
                     /translation="MSRRLEVRHMFSAHLTTARRVERPWYAWLALFLQLATALTAVPV
                     GLSLMLDPSGAGIQLPTEWIQ"
BASE COUNT          608 a         1236 c         1217 g          581 t
ORIGIN      
        1 gcggatccac gcgatcgtca cggcgactgc gccggatctc acgcccaaga cctactacgg
       61 gatgcctgcc tgggccacgc cgggcaagga tggcaaggtc atctgcttct tccagcccgc
      121 gtccaagttc aaggttcgct actgcacact cggcttccag cccgacgcga agctcgacga
      181 gggcaacatg tggccgactt cctgggcgct gaccaagctc acggccgctg acgagaagaa
      241 gatcgccgcg atggtcagga aggcggttgg ctagcgttcc ggcgcccgac tggggcgcca
      301 tcagggtccg cggcgcacgc gagaacaacc tgagggatgt cagtctcgac attcccaagc
      361 ggcgcttcac ggtcttcacc ggcgtttcgg gcagcggcaa gagttcgctc gtgttcgaga
      421 ctattgccgc ggagtcgaag cgcctgatcg acgagaccta tagcgccttc atccagggct
      481 tcatgcccac gctcgcgcgg ccggatgtcg attcgctcga gggattgacc accgcgatca
      541 tcgtcgacca ggagcgcctt ggcgcgaacg cccgatcgac cgtgggcacg gtgacggacg
      601 ccaacgccct gctgcgcgtg ctgttcagcc gactggccaa gccgcacatc ggcggcccgc
      661 aggcgttctc gttcaacatc ccgtccgtcc atgcgtcggg gaccatcacg gtcgagcgcg
      721 gcggcaaggc cgtccgcccg cccgaagatg gggagcggcg ccactacggc ggatccgtca
      781 ccgtgggcgg cggcaccggc cgtaccgaag tccgctcgtt ctcgctccag ggcggcatgt
      841 gcccgcgttg cgagggcatg ggcagcgtca gcgacatcga cctagctgag ctgttcgacg
      901 agcgcaagtc gctcgccggc ggcgccctga agatccccgg ctacacggct gacggctggg
      961 ccgtgcgtat cttcggcggg gtcgtcccgc ttgatgtgcc gatcaacaag ctcacggccg
     1021 agcaacgcca ggaattcctg tacggcgagc cacgcaaggt gcggctcgag ggcatcaacc
     1081 tgacctacga aggcctcgtg cccaagatcc agaaatcgat gctctccaag gacgtgggcg
     1141 cgctccaacc gcacatccgc gccttcgtcg aacgggcggt ggcgttcgcg acctgccccg
     1201 agtgcggcgg tacccggctg agcgagcccg cgcgcaaggc caagatcaac ggcgtcagca
     1261 tcgctgacgc gtgcgcgatg cagatcagcg atctcgccgc ctggctgcgc gacctcgacg
     1321 agccgtcggt caagccgttg ctggccaatc tccagcacct gttcgacggg ttcgtggaga
     1381 tcgggctggg ctacctgagc ctggaccgcg cgtcgggcac cttgtctggt ggtgaggccc
     1441 agcggacgcg catgatccgc cacctgggct cgtcgctgac cgatgtcacg tacgtcttcg
     1501 acgagccgac gatcggcctg catgcgcacg acgtcgagcg cacgaacgcg ctgctccacc
     1561 ggttgcgcga caagggcaac accgtgctgg tcgtggaaca cgatccagag gtgattcgca
     1621 gcgccgatca cgtcgtggac atggggcccg gcgccgggcc gcacggcggg acggtcgtct
     1681 acgagggccc cgtcgcgggt ctggctggct ctgggacggc gaccggcagg cacctcgacg
     1741 cgcgccagga gatcaaggcc gcgccgcggg cgccaaatgg tgccatcgcc atccgccacg
     1801 ccaggctgca caacctgaag gacgtgtccg tccatgtgcc gctgggcgtc ctggtcgcga
     1861 tcacgggggt ggcaggttcc ggtaagagct cactcatcca cggctgcatg ccccggaccg
     1921 atccgtccgt gacggtcatt gaccaatcgg ccatcaaggg ctcgcgccgc tcaaatcccg
     1981 cgacctacac cggcatcttg gaccccatcc gcaaggcgtt tgccaaggcg aacggcgtca
     2041 aggagtcact gttcagtgcg aactcggagg gcgcgtgccc ggcgtgcaac ggtctgggca
     2101 ggatctacac cgaccttggg ttcatggccg aggtggcaag cgtgtgcgag gtgtgcgagg
     2161 gccggcgctt caccgacgac gtgctgcatc acacgctgcg cggcaagaac atcgtcgagg
     2221 tgctggcgct gccggtcgaa caggcgcgcg acttcttcac ggagaagccc gtcacgcagg
     2281 tgctcgatcg cctcgacgcg gtgggcctgg gctacgtggc gctgggccag gagctgaaca
     2341 ccctttcggg cggcgagcgg cagcgcctga agctcgcgat cgagatggcg gccgacacgc
     2401 aggtctacgt tctcgacgag ccgaccactg gcctgcacat gcaggacgtc gagaagctga
     2461 tcgcgttgct cgaccgactg gtcgattcga gaaggacggt gatcgtgatc gagcacaacc
     2521 tcgatgtcgt cgcccgcgcc gactgggtga tcgacatggg accgggcgcc ggtcacgatg
     2581 gggggcgggt ggtattcgag ggtgcgccgg ccgatctcgc gcgcgccaag ggctcgctca
     2641 ccggccagtt cctggcgcgc catcgcgccg gcacggtcgc ggccgggcgc tgaatcgaaa
     2701 ggcgccggcc ctgcggccga cgcctcttcg ttttcgactg gcctctacgg tcagatcttg
     2761 ttgccggtat ccgagaggat ggcgttgatg ctgccaccga ggaaggtgac cgcggcaatc
     2821 gccagcacgg caatgagggc caggatcagg gcgtactcag cgaggccctg gccgcgctcg
     2881 tcacgatcga gcaggctgag gaagtaggcg agcatttcaa atcctttcgc gtggacgacc
     2941 cccattcgtc cccacgctag gaactcgcca gccggccgga tatggtgctt cagtcctgca
     3001 ccgaatggtg ccgccgtacg actacgccgt ctgggcacgc tccacgacga gcacggccac
     3061 gctcggctcg agcaggccta ggtccgccag aagcgtccgc gtctgctcgt cgccgagtgg
     3121 caggagcgca gcggtcaacg cctgacgggc ggtacggccg acacccggcc cggcaccgac
     3181 accagcgacg tcggcaaccc agccaagtga caaccggcgc atccgcacgc tcagcactgg
     3241 cccgtcaccg gtccgaaggc tgaactcgat cgtcgattcg ccacgctcca tgtgtgcaat
     3301 ctgcgcgcgg gtcgtgacag cttgcgtgtc acagccgatt cacgggctgc agataaccgg
     3361 cgcagcgggt gacgatcttc ctctcgccag gtgagcaatg gacctcgccc accgtgttcg
     3421 aacccgggag actccgctcg agtcgtcttg agccgtcgtc tggaggttcg ccacatgttc
     3481 tctgcccacc tgaccaccgc gcgtcgcgtc gagcggccgt ggtacgcctg gctggcgctc
     3541 ttcctgcagc tcgccacagc cctgaccgcg gtccccgtgg gcctgtcgct gatgctcgac
     3601 ccatccggcg cgggcatcca gctgccgacc gagtggatcc ag
//
LOCUS       DATBRX010000008         5523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_450732, whole genome shotgun sequence.
ACCESSION   DATBRX010000008 DATBRX010000000
VERSION     DATBRX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450732"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="VIF08_00365"
     CDS             complement(<1..898)
                     /locus_tag="VIF08_00365"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HEY8134464.1"
                     /translation="MKLEDFPRVSLMFGPSPVHPMPRLSAALGGGVEVWAKREDCNSG
                     IAFGGNKVRKLEYLAADAIAQGCDTLVSIGGVQSNHTRAVTGVARHLGLNVVTVQEHW
                     VDWDDPGYEVVGNIQLSRIMGGDVRMDRAGFDIGIRDSWKEALESVVNAGGKPYPIPA
                     GASDHPLGGLGFANWVLELAEQERDLGLFFDTVIVCTVTGSTHAGMIAGTALEGRGNR
                     RIVGIDASKTMDKTRDQVTRIARSTAAAIGVDRDLNDDEITLVDGYAGPIYGVPDEGT
                     IEAIQLCARTEGVITDPVYEGKS"
     gene            complement(895..1323)
                     /locus_tag="VIF08_00370"
     CDS             complement(895..1323)
                     /locus_tag="VIF08_00370"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8134465.1"
                     /translation="MRVANTVTIGAPPRDVWTQITAMEAWPEWASQMKRLERLDAGPL
                     RVGSRVRVRPKGLLANTWRVTEFEDGRLFTWETSLAPGFRLSGGHELTPVGEATRTEF
                     WLDAAGPLGAVIGPILRRTLFRRNTRNATEGLKRHLEANR"
     gene            1451..3010
                     /locus_tag="VIF08_00375"
     CDS             1451..3010
                     /locus_tag="VIF08_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEY8134466.1"
                     /translation="MEPLVAVLLILVAIAVLYALSIRVGVPYPTLFVLGGLILAFIPG
                     LPRIHLEPDLVLLVFLPPLLFIAAIETNIRELRENIAPIARLAFGLVVVTVAAVAIVV
                     QALVPGMGWAAAITLGAIVAPTDALAATSVFRRLGTPRVITALVEGEALFNDATALVL
                     YRTAVAVVAGGSVALLATGAQLVLALVGGVAIGLLAGWLAAQLLSRIDEPTVEVLISF
                     VVPFAAFLPADAVGVSGVLAAVTAGLMIGSRYGRILTARSRTLWLFSWKMVGFVLNGF
                     VFLLIGLELPTVVEALQERGRVELGGVVVIVCLVVVATRFFWTYLSSQLPNTPYRRLA
                     RLDVRLARWGRFIVGWAGLRGAVSLAAALALPRDFPERDLILLVTFAVIIVTLVGQGL
                     TLPWLVRRAGFGASNLDGDEPTMAREAAYAAGLAEVDRQRTLWSDHAPLLDRLESGLR
                     DRTQHLATSDPTETAERDQERIEHEQIQLAVIEAQRAAVIELRDSRQINDETLRQVER
                     ELDLEELRMEG"
     gene            3093..3587
                     /locus_tag="VIF08_00380"
     CDS             3093..3587
                     /locus_tag="VIF08_00380"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8134467.1"
                     /translation="MTKVYTCLWFDSGQAEEAANFYTSLIPNSRITNVSRSPADTPSG
                     PEGMVLTVDFTLDGVPYQGLNGGPDFKLNEAASIAVSCDDQAEVDQLWDALTADGGEP
                     SVCGWLKDRFGLSWQVFPRRLQELLADPDPERARRAMEAMLKMTKIETAELEAAADGA
                     PAPA"
     gene            3597..3815
                     /locus_tag="VIF08_00385"
     CDS             3597..3815
                     /locus_tag="VIF08_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134468.1"
                     /translation="MNGELLEALDHYPNGQVRFRGANLDGQMHGPWEFFRADGSLMRA
                     GSFDRGRQVGLWRTYDRAGAVVKETRFK"
     gene            3761..4846
                     /locus_tag="VIF08_00390"
     CDS             3761..4846
                     /locus_tag="VIF08_00390"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8134469.1"
                     /translation="MADIRSRGRGGQGDTVQIAARTPLALTRDQILGHRRRASALDAR
                     LPAGSASLAAAAWAGLQDSMPRAAVLSLHARVEGVAHNSWEDPALMQVWGPRFSVYVV
                     AARDRAIFTVSRYPDDARGQRVADDMAARLQALMGERRMRFDDVATGAVEGDPNRMRY
                     ATATGVVAIRWNGARQPDVWLLPRPDVSPEDARREAARRYLHVFGPSTRAAFAAWLGI
                     SAAQGRAAFDQLTAAGELIGVSTPIGDALILASDEGSFRARSDRPAAARLLPSGDTFT
                     LLKGADRELLVPDTNHRDQLWTPRVWPGALLVNGELAGTWRRDQHRLTISAWRRLSPS
                     EVSAVESEAHSLPLPGLLKPIAVTWDD"
     gene            4984..>5523
                     /locus_tag="VIF08_00395"
     CDS             4984..>5523
                     /locus_tag="VIF08_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134470.1"
                     /translation="MRGRFLAGLIFALAAMIAFAGAIPFFQSAPLVIADSRPSPDPAT
                     DAAPSSTLRSTDQGSTAAGRSTGRPAPTASPRTVDWRPLASTDPTPTPEPTDRPNPTD
                     RPDPTASPTPKPTPSSTAKPTPSPTPKPTATATPRPTASPTPAPTPTPAPKDYSGRSH
                     FWYPALNIDADWSWYGCDYG"
BASE COUNT          842 a         1902 c         1845 g          934 t
ORIGIN      
        1 tcgacttgcc ctcgtagacc gggtcggtga tcacgccttc ggttcgtgca caaagctgga
       61 tcgcctcgat cgtcccctcg tcgggcacgc cgtagatggg gccggcgtag ccgtcgacta
      121 gggtgatctc gtcgtcattc agatcccgat ccacgcctat cgcggccgcg gttgatctgg
      181 cgatgcgcgt cacctggtcg cgcgtcttgt ccatcgtctt ggaggcgtcg atgcccacga
      241 tgcgccggtt gccacgacct tcgagagccg taccggcgat catgccggcg tgcgttgagc
      301 cggtgacggt gcacacgatg acggtgtcga agaacaggcc gagatccctc tcctgttcgg
      361 ccagctccag tacccagttg gcgaagccaa ggcccccgag cgggtgatcg gatgcccccg
      421 cgggaatcgg atacggcttt ccgccggcgt tgaccaccga ctccaaagct tccttccagc
      481 tgtcgcggat gccgatgtcg aagccggccc tgtccatgcg gacgtcgccg cccatgatcc
      541 gactgagctg gatgttgccg acgacctcgt atcccggatc gtcccaatcg acccagtgct
      601 cctggacggt cacgacattc aggcccaaat gacgcgcaac gccggtcacg gcgcgagtgt
      661 gattggactg cacgccgccg atcgagacga gcgtgtcgca gccctgggcg atggcgtccg
      721 cggcgaggta ctccagcttg cgcaccttgt tgccgccgaa ggcgatgccg ctgttgcagt
      781 cctcgcgctt ggcccagacc tccactcccc caccaagcgc cgcggatagt cgcggcatcg
      841 gatgcacggg cgatggcccg aacatgagcg atacgcgcgg aaagtcctcg agcttcaacg
      901 gttcgcttcg aggtggcgtt tcaggccttc agtggcgttt cgagtgttgc gccgaaagag
      961 cgtccggcgc aggatcggcc cgattacggc acccagtggg ccggctgcgt cgagccagaa
     1021 ctcggttcgt gtggcttcac caaccggagt gagctcgtgg ccgcccgaga gtcgaaagcc
     1081 gggcgccagg ctggtttccc aggtaaacag ccgaccgtcc tcgaactcgg tcacccgcca
     1141 tgtattcgcg agcagcccct tggggcggac acgaacgcgc gagccaacgc ggagcggtcc
     1201 ggcgtcaagg cgctcgagtc gcttcatctg cgacgcccat tcgggccacg cctccatggc
     1261 ggtgatctgt gtccagacgt cgcgaggtgg cgcgccgatc gtcaccgtgt tggccaccct
     1321 caccatccgc ggatcgtaat gcagacaacc gcggacaaat tgagaacgtc gtggtcacga
     1381 cggtggcttg cggtccatcc tcgggcagct ctgcgagcca aaacggcgag cgataacctc
     1441 ccgagcagcc atggagccac tcgtcgccgt tctcctcatc ctggtagcca tcgcggtcct
     1501 gtacgcgttg tcgatcagag tgggggtgcc ctaccccact ctctttgtgc tgggcgggct
     1561 gatactcgcc ttcatcccgg gcctgccgcg catccacctc gagccggacc tcgtcctgct
     1621 tgtcttcctg ccgccgctgc tgttcatcgc ggcaatcgag acgaacatcc gcgagctgcg
     1681 cgagaacatc gcgccgatcg cgcggctcgc gtttggcctg gtcgtcgtca ccgtggccgc
     1741 agtcgcgatc gttgtccagg cgctcgtgcc gggaatgggc tgggcagcgg cgatcaccct
     1801 cggggcaatc gtcgccccga ccgatgccct cgcagcgacg agcgtgttcc gccggctggg
     1861 caccccgcgc gtgatcaccg cactcgtcga gggcgaggcg ctgttcaacg acgccacggc
     1921 gctggtcctc tatcgcacag ccgtggcggt cgtcgccggc gggtctgtcg ccctgctcgc
     1981 gaccggggcg cagctcgtcc tggccctggt cggtggcgtc gccatcgggt tgcttgccgg
     2041 ctggctggcg gcccagctgc tgagccgcat tgacgagccc acggtcgagg tgctcatctc
     2101 gttcgtcgtg ccgtttgccg cgttcctgcc ggccgatgcc gtgggtgtgt caggcgtact
     2161 ggccgcggta actgccggcc tgatgatcgg cagtcgctac gggcgcatcc tcaccgcgcg
     2221 cagtcgaacg ctgtggctgt tcagctggaa gatggtcggc ttcgtgctca acggcttcgt
     2281 tttcctcctc attggcctgg agctgccaac cgtcgtggag gcgctgcagg agcgcggccg
     2341 cgtcgaactc ggcggcgtgg tcgtgatcgt gtgcctcgtg gtggtcgcga cgcgcttctt
     2401 ctggacgtac ctgtcgagcc agctgcccaa cacgccctac cgccgactgg cgcggctcga
     2461 cgtacgtctt gcccggtggg gccgtttcat cgtgggctgg gccggactgc gtggcgcggt
     2521 gtcgctcgcc gccgcgcttg cgctgccacg cgactttccc gaacgtgacc tgatcctgct
     2581 cgtcacgttc gccgtgatca tcgtgaccct cgtcggccag gggcttacgc tgccctggtt
     2641 ggtgcgtcgc gccggttttg gcgcctcgaa tctcgacggc gacgaaccga ccatggcgcg
     2701 tgaggcagcc tacgccgccg gcttggcgga ggtggatcgg caacggacgc tctggtcgga
     2761 ccacgcgccg ctcctggacc gcctggagtc cggactgcgc gaccgcaccc agcacttggc
     2821 tacgagcgac cccacggaaa cggccgagcg ggaccaggaa cgcatcgaac atgagcagat
     2881 ccagctcgcg gttatcgagg cgcagcgcgc ggcagtcatc gagttgcgcg actcgcgcca
     2941 gatcaacgac gagactttgc gccaggttga gcgtgagctc gacctcgaag agctgcgcat
     3001 ggaaggttga gtcgcagcgt ccgtgcaacg cagggctccg ccgggagcgc tagggtgacc
     3061 agctgagttg aaagccgtct aaggagacag cgatgaccaa ggtctacacg tgcctctggt
     3121 ttgatagcgg tcaggccgag gaggcggcga acttctacac gtcgctcatc cccaacagcc
     3181 ggatcacgaa cgtgtcgcgc tcgcccgccg atacgccctc tggaccggag ggcatggttc
     3241 tgaccgtcga cttcacgctt gatggagtgc cctaccaggg gctgaacggc gggcccgact
     3301 tcaagctgaa cgaggccgcg tcaatcgcgg tctcgtgcga cgatcaggcc gaagtggatc
     3361 agctctggga cgcgctgacc gctgatggcg gcgagccgag cgtctgtggc tggctcaagg
     3421 atcgcttcgg cctgtcgtgg caggtcttcc cgcggcgcct gcaggagctg ctggccgatc
     3481 ccgatccgga gcgcgcacgg cgggcgatgg aggcgatgct caagatgacc aagatcgaga
     3541 ccgccgagct cgaggctgcg gcggacggcg cgccggcgcc ggcctaggcc cggccgatga
     3601 acggcgaact tctggaggcc ctcgaccact atcccaatgg tcaggtcagg tttcgcgggg
     3661 ccaacctgga cggccagatg cacggtccgt gggagttctt ccgcgcagac ggctcgttga
     3721 tgcgggcagg ctcgtttgac cgcggcaggc aggtcggtct gtggcggaca tacgatcgcg
     3781 cgggcgcggt ggtcaaggag acacggttca aatagccgct cgcacgccgc tcgccctcac
     3841 gcgcgaccag atcctgggcc atcgccggcg agcgagtgcc ctggatgcgc ggctgccagc
     3901 gggatccgct tcattggcgg cggccgcatg ggccgggctg caggacagca tgccgcgcgc
     3961 cgcagtgctg tcgctccacg cccgggtcga gggcgtggcc cacaactcgt gggaagaccc
     4021 ggcactcatg caggtctggg ggccgcgctt ctcggtatac gtcgtggccg cgcgcgaccg
     4081 cgccattttc acggtcagcc gctacccgga tgacgcgcgc ggtcagcggg tggccgacga
     4141 tatggccgcg cggcttcagg cactcatggg cgagcggcgc atgcgctttg atgacgtcgc
     4201 gacgggcgca gtcgagggcg acccgaaccg gatgcgctac gccaccgcca ccggcgtggt
     4261 cgcgatccgc tggaatggcg cgcggcagcc cgacgtgtgg ctgctgccac gcccggatgt
     4321 ctcaccggag gacgcgcgcc gcgaggctgc gcgccggtat ctgcatgtct tcgggccgag
     4381 cacgcgggca gcctttgccg catggctggg aatcagtgcg gcgcagggtc gggcggcatt
     4441 cgaccaactg accgccgcgg gcgagctgat cggcgtatca acgcccatcg gcgacgcgct
     4501 gatcctggcg tcagacgaag ggtcgttccg ggcgcgatcg gaccgtccgg ccgcggcgcg
     4561 acttctaccg agcggcgaca cgttcactct gctgaaggga gcggaccgtg agctgctcgt
     4621 tccggatacg aaccaccgcg accagctgtg gacgccacgt gtctggccgg gtgcgctgct
     4681 cgtcaatggt gagctggccg gcacctggcg gcgcgaccag catcgtctga ccatctcagc
     4741 ctggcggcgg ctgtcgccgt cggaggtctc agcggtcgaa tcggaggccc attcgttgcc
     4801 cctgccgggc ctgttgaagc ccatcgcggt gacctgggac gactgattcg cggcggagcg
     4861 ccagccatct gcccataacc gaccactgcc gacccggacc acacacgcac tagcccgtcc
     4921 gggccaagcc cgctagacga ccgcgtaggt gttgtcgcgt gcccttgaag gtgttctggg
     4981 ggcatgcgag ggaggttcct ggccggtctc atatttgccc ttgcggccat gatcgcgttc
     5041 gccggcgcga tcccgttctt ccagtccgcg ccgctggtga tcgcggatag ccggcctagc
     5101 ccggaccctg ccacagacgc ggccccatcg agcacactcc gctcgaccga tcagggctcg
     5161 actgccgcag ggcgcagcac cggccgcccg gcgcctacag cctcgccacg caccgtggac
     5221 tggcgtccac tcgccagtac cgatccgacc cccacgcctg agccgacaga cagacccaac
     5281 ccgacggatc ggccggaccc gaccgcgtcc ccaacgccca agcccacacc gtcgtcaaca
     5341 gccaagccga ccccctcacc gacgcccaag ccaacggcca ccgcgacgcc gcgaccaact
     5401 gcctcgccga caccggctcc gaccccgacg cctgcgccaa aggactacag cgggcgatcg
     5461 cacttctggt acccggccct gaacatcgac gccgactggt cgtggtacgg ctgcgactac
     5521 ggc
//
LOCUS       DATBRX010000009         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_115565, whole genome shotgun sequence.
ACCESSION   DATBRX010000009 DATBRX010000000
VERSION     DATBRX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115565"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1163
                     /locus_tag="VIF08_00400"
     CDS             180..1163
                     /locus_tag="VIF08_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY8134471.1"
                     /translation="MPAAIGLSPILSARYRPQDLARIADAAPGSRLVPVSLEGLADGD
                     LADIEVLLRGPLPAGVFDRLLSRTPNLKWVHSATAGVERVLTPAALERGLAITNARGV
                     FSDPIAEYVLMAVLAVSRRLPQLLELQSERTWQPLEGREMSDLTIGVVGLGSIGRRVA
                     ELGVKFGCRVIAIRRGTGGESAEGVEGIAEVLTPDHLPELMAQSDFVVLALPLTSDTQ
                     DLFNDAMFATAKPGGWLINVARGALVDERALVRAVREGRMGGAVLDAFREEPLPSDSE
                     LYSTPNIIVTPHTSWSSGRVLDRSIELFCDNLGRFARGEPLLNLVDPGAGY"
     gene            1183..>1884
                     /locus_tag="VIF08_00405"
     CDS             1183..>1884
                     /locus_tag="VIF08_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY8134472.1"
                     /translation="MQVAIVGLARSGKTTVFNTLTRGNAETGGFGGMTVNVGVVKVPD
                     ERLTKLTELFKPKKEIAADVTYVDLPAPPTSADGIEAAEIPADQLARLRTADELLHVV
                     RAFEDPSVPHPDGSVDPWRDIERLDLEFVLADLSQVDKRIEKLRQQGHHGSAQEREAN
                     DRELAVLEKLAPALREGNPIRDIELGEDEAKRIRGFRFLTEKPVLLLLNIGEADIANA
                     ADLAAGIAKRYLHKHS"
BASE COUNT          289 a          651 c          650 g          294 t
ORIGIN      
        1 atcgcggccg acggcggcca ggccgcgatc gatgccctgt ggcgcgggcc ttcgtcgctg
       61 ccgagcgaag ctgagctggc cgatcctgcg gcctggctgc gacgcgtcgt gcccagcgca
      121 ctcgcgcggc cggcgtgaca agcatgcgcc aaagcggccc cggcggcggt cccaacggag
      181 tgccggctgc gattggcctg tcccccatcc tgtctgctcg ttatcgcccg caggacctgg
      241 cgcgcatcgc cgacgcggcg cctgggagcc ggctcgtgcc ggtctcgctc gagggcctgg
      301 ccgacggcga cctagcggac atcgaggtgc tgctgcgcgg cccgctgccg gccggcgtct
      361 tcgaccgtct gttgagccgc acgccgaacc tcaagtgggt ccactcggcg accgccgggg
      421 ttgagcgcgt cctgacgccg gccgcgctcg aacgcggtct cgcgatcacc aatgcgcgtg
      481 gcgtgttcag tgatcccatc gcggaatacg tcctaatggc agtgctggca gtgagccgcc
      541 ggctgcctca attgctcgag ctgcaaagcg aacgcacgtg gcagccgctc gagggtcgcg
      601 agatgagcga cctgacgatc ggtgtggtgg gcctgggctc gatcggccgc cgagtggcgg
      661 agctcggcgt caagttcggc tgtcgggtga ttgccatcag gcgcggcacc ggcggtgagt
      721 cggccgaggg cgtcgagggc atcgccgagg tgctgacacc cgatcacctg cccgagctga
      781 tggcgcagag cgacttcgtc gtgctggcgc tgccgctcac gtcggacacg caggacctgt
      841 tcaatgacgc gatgttcgcg accgccaagc ccggcgggtg gctcatcaac gttgcgcgcg
      901 gcgccctggt cgacgaacgc gccctcgttc gcgcggtgcg cgaggggcga atgggcggcg
      961 ccgtcctcga cgcattccgc gaagagccgc tgccgtccga ttcggagctc tactcgacgc
     1021 ccaacatcat cgtcaccccg cacacgtcgt ggtcgtcggg ccgggtgctc gatcgcagca
     1081 tcgagctctt ctgcgacaac ctgggccgct tcgcgcgcgg cgagccgctg ctcaacctgg
     1141 tcgatccggg cgcgggttac tgatccgccc tagagtcccg gggtgcaggt agcaatcgtt
     1201 ggccttgccc gcagcggcaa gacgaccgtg ttcaacacgc tcacgcgcgg caacgctgaa
     1261 acgggcggct ttggcggcat gacggtcaat gtcggcgtgg tcaaggtgcc cgacgaacgg
     1321 cttacgaagc taaccgagct cttcaagccc aagaaggaga tcgcggccga cgtcacctat
     1381 gtcgatttgc cggcgccgcc gacctcagcg gatggcatcg aggccgcgga gatcccggcc
     1441 gaccagctcg cgcggctgcg caccgcggat gagctgctcc acgtcgtgcg tgcattcgag
     1501 gatccgtctg tgccccaccc cgacggctcg gtcgatccgt ggcgcgacat cgagcggctc
     1561 gacctggagt tcgtgctagc cgacctgtcc caggtcgaca agcgcatcga gaagctgcgc
     1621 cagcagggtc atcacggtag cgcacaggag cgcgaggcga acgatcgcga gctcgcggtc
     1681 ctcgagaagc ttgcgcccgc gctgcgcgag ggcaacccca ttcgcgacat cgagctcggc
     1741 gaggacgagg ccaagcgcat ccgcggcttc cggttcctga cggagaagcc ggtgctgctc
     1801 ctgctcaaca tcggcgaggc cgatatcgcc aacgcagccg accttgccgc cggaatcgcc
     1861 aagcgctacc tgcacaagca ctcc
//
LOCUS       DATBRX010000010         8675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3467931, whole genome shotgun sequence.
ACCESSION   DATBRX010000010 DATBRX010000000
VERSION     DATBRX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3467931"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..592)
                     /locus_tag="VIF08_00410"
     CDS             complement(209..592)
                     /locus_tag="VIF08_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134473.1"
                     /translation="MPPEVNVATNPLIVYGMVAVFSVAIVLALVFARPRRGLGAVKRS
                     EVPDQLSGGLPWMPGGDIRGNDPQPEPEPLPATDPWQPAIDPWQQPTADQWPAADPWQ
                     PAPPAAPPPTGAAGQSEPQPRWDKR"
     gene            618..1952
                     /locus_tag="VIF08_00415"
     CDS             618..1952
                     /locus_tag="VIF08_00415"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HEY8134474.1"
                     /translation="MARVQGLDRPPFVIRARLISPLDAGGMTHLADALIEVDAEGRIG
                     RAEPWPADSGASANASKSGVVDLRPMVVLPGLVDLHAHLPQLPNAGVGAGLDLLTWLE
                     RYIFPLERDFDAAAAERLAPLVFKALAAAGTTTALLYGAVYEASLDVCFAAAEAHGIR
                     LVLGKVMMDRLRYDATLGDKEVLDASLAQSQRLIERWHGRDRGRLRYAVTPRFAVSCS
                     PEMLRESAALAKSTGAYWQTHIAEDRHEVAEVRRLFPDASDYTDVYDRAGGLGPQTIL
                     AHAVYLSDREVTRLAETGTRIAHCPASNLFLSSGAMPLARYLDAGLVVGLGSDVAAGP
                     ELSIFASMRAGAYTQSGVHTMLGDERPLLKPLDWLRMGTLEGARVLGMERETGSIEAG
                     KDADFIVVDPAATAPIEGAQVDDPSDIVSRLMYRTREDMIRGAWVRGRRLPS"
     gene            complement(1967..2608)
                     /locus_tag="VIF08_00420"
     CDS             complement(1967..2608)
                     /locus_tag="VIF08_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134475.1"
                     /translation="MLVVSRACALIVFALVIGCGGAQPGSSPTASPATSAVPSVPVTT
                     APAPTPSPTQPNVPTPGCCVDPTLSDAGIAAQVTIVNDTRGHRDGTHDIYGVAADGSE
                     CDGSFEEPDFIVVAWDDDAPDGSIHRFGISVGADDIPAADGSTADITDGSVSFDFVSA
                     SGFGTTYTGAAMRENDGSSTISVTRSGTMLTFHFDGVTWDNVGFGGEFVCINA"
     gene            2694..4280
                     /locus_tag="VIF08_00425"
     CDS             2694..4280
                     /locus_tag="VIF08_00425"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEY8134476.1"
                     /translation="MASCDLLIDGGTVVDGSGAPGFVAGVAISGDRLSILAPGAERPV
                     ATRTIDATGKVVAPGFIDLHSHAGLMILADPRHEPKVRQGVTTEIIGVDGLSYAPLPS
                     RQDLEDLIEMNAGLDGAPDIPADWESVAEYLDRFESSVSVNVGLLIGNSALRISRLGW
                     NDVPVNAAALADMKALLADAMAAGALGLSSGLDYPPGAFAGTDELAALTGEAARHGGF
                     YHTHVRYPLGDRYLDPFREAIEIGRRGGGPTHITHFYHRETHPGGPEQLLSLVGDARA
                     EGLDVTFDCYPYEWASTRLLIQMPLWIQEGGPRPLKERLADASARERLRAELRARGAA
                     YTSPAGWADVRLGYFANPDNLRWEGRTLADVMAERGVDAVDAMCDLLLSEGLRVNQVT
                     SGPWTKTLHHFVVHPVGMFGTDSTFVGAKPSPRTYGSFPRVLGEFVRDRALLGLEEAV
                     RKMTSAPAARLGLADRGQLKDGFLADVVVFDPQTIASNATYDEPRRFPTGIEHVLVNG
                     TLVVDGGEHTGALPGRVVRR"
     gene            complement(4285..4818)
                     /locus_tag="VIF08_00430"
     CDS             complement(4285..4818)
                     /locus_tag="VIF08_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134477.1"
                     /translation="MDWLLAIPLFLHVMGAIVAFGPSFAMALMGPAAAAEPMHMNFTL
                     RLQKKIASTLITPLALLQGVTGLLLVFRLADLRDQSFIDVLLWRDGAGANHYWLAVAI
                     VLYIAALAISFMRIYPNLRILLDGTSGPPPAPPAGGAPAGPPPHIMAAAKSIRMASLT
                     NLVLIVSIVFLMVTKPF"
     gene            4884..7337
                     /locus_tag="VIF08_00435"
     CDS             4884..7337
                     /locus_tag="VIF08_00435"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8134478.1"
                     /translation="MTVTTRPPRVAPTIDAPAAPLPAPAPGELQIGRARGLPLRREGP
                     DKLTGTALYTDDMVFPGAWYGHTVRSTIAHARLLGIDLAPEFDWSKVVVLTAKDVPGE
                     NVMSLITDDQPVLVSDEIRHYAEPVALLAAPDRETLREAVKHISLRTEPLPAVFDPLV
                     SQQEFAHFALDKGGDVDAALAAADLVIEGTYRVGHQEQLYIENNAMIAVPRPDGGVTI
                     HGSMQCPYYIHKALKRALGLSGEQAVVIQAETGGGFGGKEEFPSVIATHAGMLALRVG
                     KPVRMIYDRHEDLAATTKRHPAIVTHRTGVMRDGTLVAQDIDVVMDGGAYCTLTPVVL
                     SRGTLHAGGPYSCPNVRIRGRAMATNTPPNGAFRGFGAPQTEFAAEMQVNRIAEALQI
                     SPLELRRKWAYQLGDTTPSEQVLRESVAAHEVLERAAEAAEFERIKSQTARQRQERQA
                     GAKSAVLSSPSQKTARGIGLAMAWHGAGFTGSGEVYLASVASLELSASGEIRVLTAST
                     EMGQGTKTIFPQLAAEELGVPIEAVGMAPQDTSIVPDSGPTVASRTAMVVGGLVIKAA
                     RRMRAAVEEATGRPFAESYVDYARDHNGLRIDQQFEAYPNVHFDDKTYKGDAYPAYGW
                     AACVAEVDVDLDTGEVTVRNVVSANDIGHVIHPVLAEGQVEGGTLQAVGYATIEEIKL
                     ADGRYLNDRLATYIIPTALDAPRIDSILVESPFSGAPHGAKGVGELPMDVPAAAVVAA
                     IHEATGAWIHDLPASPERILAALHGITPPPPPGISTVSERSAKAAPSAADAGTPQVGL
                     DPKPDDRTDPQPMRGPYDG"
     gene            7334..7762
                     /locus_tag="VIF08_00440"
     CDS             7334..7762
                     /locus_tag="VIF08_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8134479.1"
                     /translation="MNNHDTRGAPPEGYQTVSPYLLYEDADRAVAWLTQGFGFVERRV
                     TTGAAGRSHHELILGTDGLVMLGQMGPAFKSLKTLGVDSPAMVHVYVEDVDALHQRAK
                     TAGADVTSLEISPAGDKRFMASDPEGITWVFAQRISGEAL"
     gene            7759..8226
                     /locus_tag="VIF08_00445"
     CDS             7759..8226
                     /locus_tag="VIF08_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8134480.1"
                     /translation="MSYRFTVNGAEQALDVPGMRRLLDALREDLGLTGTKEGCGEGEC
                     GACSVLIDGELANSCLVPLAQVEGAKVVTVEGLASQGGLDRLQQAFLETGGAQCGICT
                     PGMLMAGRAYIAAGGGPDEDAIREAIAGNLCRCTGYTKIIDAIALAAAATPSP"
     gene            8226..>8675
                     /locus_tag="VIF08_00450"
     CDS             8226..>8675
                     /locus_tag="VIF08_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY8134481.1"
                     /translation="MRVEPPIASPATLAEAFALLASGEVWRPIAGGTDLIVQMTGDLA
                     PPPERVLDIWRIPELRGITVGDGALTMGALTTYTQIRYSDACREHLPALWEAAATIGA
                     AQIQNRGTIGGNIANASPAGDTFPALAVYEATVRVVGPGGRRAVPMAE"
BASE COUNT         1394 a         2947 c         3031 g         1303 t
ORIGIN      
        1 tcgttccagc gcgtgtagaa cgggtgcttg agaaggtggc gctcgtcgat catcgcgtcg
       61 aggcggtcaa gcagattcat cggtgtcttt ctcctgttcg cagcttccgc ggagtctagc
      121 aagcgacggg acggcaccga cgattcgtcg gggccggacc aactgtcgcg cgcagcagcg
      181 gagctgcgga gagaaatggc ctggagttct agcgcttgtc ccagcgcggt tgcggttctg
      241 actggccggc agcgccggtc ggcggcggag cggcgggcgg cgcgggctgc cacgggtcgg
      301 cggcgggcca ctgatcggcc gtgggctgct gccagggatc gattgctggc tgccacgggt
      361 cggtcgcggg caaaggctca ggctcgggct gcgggtcgtt accgcggatg tcgccaccgg
      421 gcatccacgg caggccacct gacagctgat cgggaacttc cgatcgcttg accgcaccca
      481 ggccgcgccg cggccgggcg aacaccagcg cgagcacgat cgccacgctg aacacggcga
      541 ccatgccgta cacgatcagc ggattggtcg cgacgttgac ctcgggtggc atctcgcgcc
      601 agagtgtcgg acctaacatg gcgcgcgtgc aaggtctcga ccgaccgccg ttcgtcattc
      661 gcgcccggct gatcagcccg ctcgacgcgg gcggcatgac gcacctcgcc gacgcgctga
      721 tcgaggtcga cgcagaaggt cgtattgggc gcgccgagcc gtggccggct gattcgggtg
      781 catcggccaa tgcgtcgaaa tcgggcgtcg tcgacctccg gccgatggtc gtgctgcccg
      841 gcctggtcga cctccacgcc cacctgccgc agctgcccaa cgccggggtt ggcgccgggc
      901 tcgacctctt gacgtggctg gagcgctaca tcttcccgct ggagcgcgac tttgacgcgg
      961 ccgcggccga gcgcctggcg ccgctcgtct tcaaggcact ggccgcggcc ggcaccacga
     1021 ccgcgctgct ctacggcgcc gtctacgagg ccagcctcga cgtctgcttt gccgcggcgg
     1081 aggcccacgg cattcgtctc gttctgggca aggtgatgat ggatcggctg cgttacgacg
     1141 cgacgctcgg cgacaaggag gtgctcgacg ccagcctcgc ccagtcgcag cggctgatcg
     1201 aacgctggca tggccgcgat cggggccggt tgcgctacgc cgtcacgccg cgcttcgcgg
     1261 tcagctgctc accggagatg ctgcgcgagt cggcggcgct ggccaagtcc acgggcgcgt
     1321 actggcagac ccatatcgcc gaggatcgcc acgaggtggc ggaggtccgc cgcctcttcc
     1381 ctgacgccag cgactacacc gacgtgtacg accgcgccgg cggcctgggg ccgcagacca
     1441 tccttgccca cgcggtgtac ctgtcggacc gcgaggtgac gcgactggcc gagacgggca
     1501 cgcgcatcgc ccactgcccg gcgtcgaacc tgttcctgtc gagcggcgcg atgccgctgg
     1561 cacgctacct cgacgccggg ctggtcgtcg gcctggggag cgacgtcgcc gcggggccgg
     1621 agttgtcgat cttcgcttcc atgcgcgccg gggcatacac ccagagcggc gtgcacacga
     1681 tgctcggcga tgagcgaccc ctgctcaagc cgctcgattg gctgcgcatg ggcacgctcg
     1741 agggcgcgcg cgtcctgggc atggagcgcg agacaggctc catcgaggca ggcaaggatg
     1801 cggacttcat cgtcgttgac ccggccgcga ccgcgcccat cgaaggcgcg caggtcgacg
     1861 atccgtcgga catcgtgagc cggctgatgt accgcacccg tgaggacatg atccgcggcg
     1921 cctgggtgcg cggccggcga ctgcccagct gagcagttcc tcagggtcag gcgttgatgc
     1981 agacgaactc gccgccgaag cccacgttat cccacgtgac gccgtcgaag tggaaggtga
     2041 gcatggtccc ggagcgcgtg acggagatcg tcgacgagcc atcgttctcg cgcatcgctg
     2101 cgcccgtgta ggtcgtcccg aaccctgacg cggagacgaa atcgaagctc acgctgccgt
     2161 cggtgatgtc ggcggtgctg ccgtccgcgg ccgggatgtc gtcagcgcca acgctgatgc
     2221 cgaagcggtg gatcgagccg tcgggggcat cgtcgtccca ggcgacgacg atgaagtcgg
     2281 gttcctcgaa tgagccatcg cactcgctac cgtcggctgc gacgccatag atgtcgtgtg
     2341 tgccatcacg gtggccgcga gtgtcgttga cgatcgtcac ctgggcggcg atgccggcgt
     2401 cactgagagt gggatcgacg cagcacccgg gtgtaggcac gttgggttgc gtgggtgagg
     2461 gcgtcggcgc gggcgccgtg gttaccggga cgctgggaac ggcgctggtc gcaggtgatg
     2521 ccgttggact cgaacctggt tgagctccac cgcatccgat taccagggcg aagacgatca
     2581 gggcacaggc gcgagatacg accaacatca ggatcctcgg actactcgga cgcggcaatc
     2641 cgcgaggtga accgcgagct tgattctaga cctgaccgct agccttgccg gcgatggcga
     2701 gctgcgatct gctgatcgat ggtgggactg tcgttgacgg cagcggcgcg cccgggttcg
     2761 tcgctggcgt cgcgatcagc ggcgaccggc tgagcatcct cgcgcccggc gctgagcgac
     2821 cggtcgcgac gcgaacgatc gacgcgaccg gcaaggtggt agcgccgggg ttcatcgacc
     2881 tgcactcgca tgccgggctg atgatcctgg ccgatccacg ccacgagccc aaggtgcgcc
     2941 agggcgtgac gaccgagatc atcggcgtcg acggcctctc gtacgcgcca ctcccctcgc
     3001 gccaggacct cgaggacctg atcgagatga acgccgggct cgatggcgca ccggacatcc
     3061 ctgctgactg ggaatcggta gccgagtacc tcgaccgctt cgaatcgtcc gtcagcgtca
     3121 acgtcggcct gctgatcggc aattcggcgc tgcgcatctc gcgcctgggt tggaacgacg
     3181 tcccggtgaa cgcggccgcg ctcgccgaca tgaaggcgct gctcgccgac gcgatggccg
     3241 ccggtgcgct gggcctgtcg agcggtctcg actacccgcc cggcgcgttt gccggcaccg
     3301 acgagctcgc cgcgctgacc ggcgaggcgg cgcgccacgg cggcttctac cacacgcacg
     3361 tgcgctaccc gctcggtgat cggtacctcg atccgttccg cgaggcgatc gagattgggc
     3421 gccgtggcgg cggcccgacg cacatcaccc acttctatca ccgcgagacg catcccggcg
     3481 ggcccgaaca gctgctcagc ctcgtcggcg acgcgcgcgc cgagggcctc gacgtgacgt
     3541 tcgactgcta cccgtacgag tgggcgtcga cgcggctgct catccagatg ccgctctgga
     3601 tccaggaggg cgggccgcgg ccgttgaagg agcgcctcgc ggatgcatcg gcgcgcgagc
     3661 gattgcgcgc ggagctgcgc gctcgcggcg ccgcgtacac gagccccgcc gggtgggccg
     3721 atgtgcggct gggctacttc gcgaacccgg acaacctgcg ctgggaaggc cggacgctgg
     3781 ccgatgtcat ggccgaacgc ggcgtcgacg cggtcgacgc gatgtgcgac ctgttgctca
     3841 gcgagggcct ccgcgtcaac caggtgacgt caggaccatg gacgaagacg ctccaccact
     3901 tcgtcgtcca tccagtaggc atgttcggca cggactcgac gttcgtcggc gcgaagccgt
     3961 cgccacggac ctatggctcc ttcccgcgtg tgctgggtga gttcgtgcgc gaccgggcgc
     4021 tgcttggcct cgaggaggcg gtgcgcaaga tgacctccgc gcccgcggcc cggctcggtc
     4081 tcgcggaccg cggccagctg aaggacggct tcctcgccga cgtcgtcgtt ttcgatccgc
     4141 agacgatcgc gtcgaacgcg acgtacgacg agccgcgccg ctttccgacc gggatcgagc
     4201 acgtgctcgt caacggcacg ctggtcgtcg acggtggcga gcacaccggg gcgctgcccg
     4261 gccgggttgt gcgccgctag ggcactagaa cggttttgtc accatcagga agacgatcga
     4321 gacgatcagc accaggttgg tgaggctggc cattcgaatg ctcttagccg ccgccatgat
     4381 gtgcggcggc ggaccggcgg gcgcgccacc agccggagga gccggcgggg gaccgctcgt
     4441 gccgtcgagc aggatgcgca ggttcgggta gatgcgcatg aacgagattg ccagcgccgc
     4501 gatgtacagc acgatcgcga ccgccaacca gtagtgattc gccccggccc catcgcgcca
     4561 cagcagcacg tcgatgaatg actgatcacg gaggtcggcc agtcggaaaa cgagcagtag
     4621 gccggttacc ccctgcaaga gggcgagtgg cgtgatcagc gtcgacgcga tcttcttctg
     4681 caagcgcagc gtgaagttca tgtgcatcgg ctcagccgcg gccgcaggcc ccatgagcgc
     4741 catggcgaac gacggcccaa acgcaacgat cgcacccatt acgtgtagaa aaagcgggat
     4801 cgccagcagc cagtccacgc tcgaaatctc ctcattcgcg gtatcgcagg agagcccacg
     4861 ttagcactac catcagccgt catatgaccg tcaccacgcg tccgccgcgc gtcgcaccca
     4921 cgattgacgc acctgccgcg cccctgcccg cgcccgcgcc cggtgaactg cagatcggcc
     4981 gagcgcgcgg gctgccactg cgccgcgagg gtcccgacaa gctgaccggg acggcgctgt
     5041 acacggacga catggtcttc ccgggggcgt ggtacgggca cacggtgcgc tcgacgatcg
     5101 cgcacgccag gctgctgggc atcgacctcg cgccggagtt cgattggtca aaggtcgtcg
     5161 tcctgacggc caaggatgtg ccgggcgaga acgtgatgag cctcatcacc gacgaccagc
     5221 cggtgctcgt ctcagacgag atccgccact acgcggagcc ggtggcgctc ctcgccgcgc
     5281 ccgaccggga gacgctgcgc gaggcggtca agcacatcag cctgcgcacc gaaccactgc
     5341 ccgcggtgtt cgacccgctc gtgtcgcagc aggaattcgc gcactttgcg ctcgacaagg
     5401 gcggcgacgt cgacgcggcc ctcgcggccg cggacctggt gatcgaaggc acgtaccgcg
     5461 tcggccacca ggagcagctg tacatcgaga acaacgcgat gatcgcggtg ccgcggcccg
     5521 acggcggggt cacgatccac gggtcgatgc agtgtccgta ctacatccac aaggcgctga
     5581 aacgggcgct cggcctgagc ggcgagcaag cagtcgtcat ccaggccgaa accggcggcg
     5641 gctttggcgg caaggaagag tttccgtccg tcatcgcgac gcacgccgga atgctggcgc
     5701 tgcgcgtcgg caagccggtg cggatgatct acgaccgcca cgaggacctc gctgcaacga
     5761 cgaagagaca cccggcgatc gtcacgcatc gcaccggtgt catgcgtgat ggcacgctgg
     5821 tggctcagga catcgacgtg gtgatggacg gcggcgctta ctgcacgctc acgcccgtcg
     5881 tgttgtcgcg cggcacgctc cacgccggcg gcccgtactc gtgtcccaac gtcaggatcc
     5941 gcggccgcgc gatggcgaca aacacgccgc ccaacggcgc gtttcgcggc tttggcgcgc
     6001 cgcaaaccga gttcgcggcc gagatgcagg tcaaccgcat cgccgaggcc ctgcagattt
     6061 cgccgctcga gctgcgccgc aaatgggctt accagctggg cgacaccacg ccgtccgagc
     6121 aggtgctgcg cgagagcgtc gccgcgcacg aagtgctcga acgagcggcg gaggcagcgg
     6181 agttcgagcg aatcaaatcg cagaccgccc gccagcggca ggaacggcag gcgggcgcaa
     6241 agtcggctgt cctttcctcc ccgtcgcaga agaccgcgcg cggcataggc ctcgccatgg
     6301 cctggcacgg cgccggcttc acgggcagcg gcgaggtcta cctggcgagt gtcgccagcc
     6361 tggagctgag cgcgagcggc gagatccgcg tcctgaccgc gtcaacggag atgggccagg
     6421 gcaccaagac gatcttcccc cagcttgcgg ccgaggagct gggtgtgccg atcgaagcgg
     6481 tcgggatggc gccacaggac acgtccatcg tccccgattc cggccccacc gtggcgagcc
     6541 ggacggcgat ggtcgtcggc ggcctcgtca tcaaggccgc gcggcggatg cgcgccgcgg
     6601 tcgaggaagc gacgggtcgc cccttcgcgg agtcgtacgt cgattacgcg cgcgaccaca
     6661 acgggctgcg catcgaccag cagttcgagg cgtatcccaa cgtccacttc gacgacaaga
     6721 cctacaaggg cgacgcgtac ccggcgtacg gctgggcggc gtgcgtcgcc gaggtggatg
     6781 tcgatctcga cacgggcgag gtcacggtgc gcaacgtggt gtccgccaac gacatcggtc
     6841 acgtcatcca tcccgtcctg gcggaggggc aggtcgaagg cggcacgctg caggccgtcg
     6901 gctacgccac gatcgaggag atcaagctcg ccgacgggcg ctacctgaac gatcgcctcg
     6961 ccacgtacat catcccgacg gctctggacg cgccgcgcat cgattcgatc ctcgtcgaat
     7021 cgcctttctc cggcgcgcca cacggcgcga agggcgtggg cgagctgccc atggacgttc
     7081 ctgccgcggc cgtcgtcgcg gcgatccatg aagcgaccgg cgcctggatc cacgacctgc
     7141 cggcgtcacc ggagcgcatc ctcgcggcgc tccacggcat cacgcctccg ccgcctccgg
     7201 gcatctcgac cgtgtcggag cgctcggcca aggcggctcc gtcggcagct gatgctggaa
     7261 cgccacaggt cggcctggat cccaagccgg acgatcggac cgacccgcaa cccatgcgcg
     7321 ggccgtacga cggatgaaca atcacgacac gcgcggcgct ccgcccgagg gataccagac
     7381 ggtctcgccc tacctcttgt acgaggacgc tgatcgggcc gtggcgtggc tgactcaagg
     7441 gttcggcttc gtcgagcgcc gggtcaccac gggcgctgcc gggcgaagcc accacgaact
     7501 catccttgga accgacgggc ttgtgatgct gggccagatg ggacccgcgt tcaagagctt
     7561 gaagacactc ggggtcgatt cgccggcgat ggtgcatgtc tacgtcgagg acgtcgatgc
     7621 cctccaccag cgggcgaaaa ctgccggcgc cgacgtcacg agtctcgaga tctcaccggc
     7681 tggcgacaag cggtttatgg ccagcgatcc ggagggaatt acctgggtgt tcgcgcagcg
     7741 gatttcggga gaggcgctgt gagctatcga ttcaccgtca acggtgccga gcaggcgctc
     7801 gatgtgccgg gcatgcgccg gctgctcgac gcgctgcgcg aggatctggg gctgacgggc
     7861 accaaggagg gctgcggcga gggtgagtgc ggcgcgtgca gcgtgctcat cgacggtgaa
     7921 ctggcgaatt cctgcctggt gcccctcgcg caggtcgagg gcgccaaggt cgtcacggtc
     7981 gaaggactcg cgtcgcaggg cggactcgat cggctgcagc aggcgtttct cgaaacgggc
     8041 ggcgcgcagt gcggcatctg cacgccgggc atgctgatgg ccgggcgggc atacatcgct
     8101 gctggcggcg gtcccgatga ggacgcgatc cgcgaggcga tcgcgggcaa cctgtgccgc
     8161 tgcaccggat acaccaagat catcgacgca atcgcgctcg ccgctgcggc cacgcccagc
     8221 ccctgatgcg agtcgagccg cccatcgcgt cgcctgcaac gctcgcggag gcgtttgccc
     8281 tgctggcgag cggcgaggtt tggcggccga tcgcgggcgg cacggacctc atcgtccaga
     8341 tgacgggcga cctcgcgccg ccacccgaac gagtcctcga catctggcgc atccccgagc
     8401 tgcgcggcat cacggtcggg gacggcgccc tgacgatggg cgcgctcacg acctacaccc
     8461 agatccgcta ctccgatgca tgccgtgaac acctgccggc gctctgggag gccgcggcga
     8521 ccatcggcgc ggcgcagatc cagaaccggg gcacgatcgg cggaaacatc gccaacgcct
     8581 cgcccgcggg cgacaccttt cccgctctcg ccgtgtacga ggcgaccgtg cgcgtcgtcg
     8641 gccccggcgg ccggcgcgcc gtcccgatgg ccgag
//
LOCUS       DATBRX010000011         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1903938, whole genome shotgun sequence.
ACCESSION   DATBRX010000011 DATBRX010000000
VERSION     DATBRX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903938"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VIF08_00455"
     CDS             <1..207
                     /locus_tag="VIF08_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134482.1"
                     /translation="GRAKTLLKKAFETQEAKRGFSIVEILSNCPVGWGMTAPTSMEHV
                     EKDVIETYPLGVLVDRSLAAAEAH"
     gene            207..794
                     /locus_tag="VIF08_00460"
     CDS             207..794
                     /locus_tag="VIF08_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY8134483.1"
                     /translation="MQHGTIVAGFGGQGILFCSSVLAQAALLEGREVSWMPSYGPEMR
                     GGTASCTVIVADRPIGSPTLDAADSAIVLNPPSLAKFEPLVAHGGLIVINSSLVEAQV
                     TRTDIEALLVPCTQIARDAGDDRLVSIVALGGLIARRPIVERASVEAAIRELLGRKKA
                     HMIDADIAAFELGHAAGMSGSAREQDERVLAEVWR"
     gene            860..1390
                     /locus_tag="VIF08_00465"
     CDS             860..1390
                     /locus_tag="VIF08_00465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY8134484.1"
                     /translation="MEPLAVTVRPERPADRDAIRALHDLVFEEPDEGEIVDAIRGSAD
                     EIAGGSIVALDKSGAVIGHLLASRGFIVGSDGTQRPVWMLGPFAVRADLQGRGVGARL
                     MREQINRATKRGVPLICLLGHAAYYPRFGFQSARSMGIEPPQPWDDEHWQALRLAAYS
                     PDYKGRASYAAPFNVG"
     gene            complement(1392..1467)
                     /locus_tag="VIF08_00470"
     tRNA            complement(1392..1467)
                     /locus_tag="VIF08_00470"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1432..1434),aa:His,seq:gtg)
BASE COUNT          264 a          548 c          570 g          251 t
ORIGIN      
        1 ggccgcgcca agacgctgct caagaaggcg ttcgagacgc aggaagccaa gcgcgggttc
       61 tccatcgtgg agatcctgtc caactgcccg gtcggctggg gcatgacggc gccgacatca
      121 atggagcacg tagaaaaaga cgtcatcgag acctacccgc tgggcgtgct cgttgatcgc
      181 tcccttgccg cggcggaggc gcactgatgc agcacggcac gatcgtcgcc gggttcggcg
      241 gccagggcat cctgttctgc agcagcgtgc ttgcgcaggc ggcgttgctc gagggccgcg
      301 aggtgtcgtg gatgccgtcg tacggccccg agatgcgcgg cggcaccgcc tcgtgcacgg
      361 tcatcgtcgc cgaccggccc atcggctcgc caacgctgga tgcggccgac tcggcgatcg
      421 tgctcaaccc gccgtcgctc gccaagttcg agccgctcgt cgctcacggc gggctcatcg
      481 tcatcaactc gtcactggtc gaggcacagg tcacgcggac cgatatcgag gcgctcctgg
      541 tgccgtgcac gcagatcgcg cgcgacgccg gcgacgatcg cctcgtcagc atcgtcgcgc
      601 tgggcgggct gatcgcgcgc cggcccatcg tcgagcgcgc gtcagtcgag gccgcgatcc
      661 gcgagctgct cggtcgcaag aaggcgcaca tgatcgacgc cgacatcgcg gcgttcgagc
      721 tgggccacgc ggcaggcatg tcgggcagcg ccagggagca ggacgagcgc gtcctcgccg
      781 aggtctggcg ctagctccgg ccgctcgcgc caccagggcg ggtggcgtca ggacaggctg
      841 agcggctaac ctgcaagcca tggaaccgct ggcggtcacc gtgcgccccg agcgtccggc
      901 cgaccgggac gcgatccgcg cgctccacga tcttgttttc gaggagcccg atgagggcga
      961 gatcgtcgac gccatacgcg gcagcgccga tgagatcgcg ggcgggtcca tcgtcgcgct
     1021 cgacaagagt ggcgcggtca tcggccatct gctggcatcg cgcggcttca tcgtcggcag
     1081 cgacgggacg cagcggccgg tctggatgct cggcccgttc gcggtgcgcg ccgacctcca
     1141 gggccgcggt gtgggcgcgc ggctgatgcg cgagcagatc aacagggcca ccaagcgcgg
     1201 cgtgccgctg atctgtctgc tcggccatgc ggcgtactac ccgcgcttcg gcttccagtc
     1261 cgctcgatcg atgggcatcg aaccgccgca gccgtgggac gacgagcact ggcaggcgct
     1321 cagactcgcg gcatacagcc ccgactacaa gggcagggcc agctacgcgg cgccgttcaa
     1381 cgtggggtaa atggggtggc caatgggggt cgaacccata accttcggat ccacaatccg
     1441 atgctctgcc ggttgagcta tggccaccgc gggggagcgc caagtatcgc ctaaccttgg
     1501 caaatgaaca tcccgggctt catcgcgcgt cagttctacg ttgccggatc gctgcgcaac
     1561 accgagtcgg gtttcgagct caaggcgcag aacccgatgg gcaatggagt gctcaccggc
     1621 gtcggtcacc taa
//
LOCUS       DATBRX010000012         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_340167, whole genome shotgun sequence.
ACCESSION   DATBRX010000012 DATBRX010000000
VERSION     DATBRX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340167"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..2289
                     /locus_tag="VIF08_00475"
     CDS             343..2289
                     /locus_tag="VIF08_00475"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY8134485.1"
                     /translation="MSLGSTAGLTPWDRWLELTAQIDGFPRHLSIHTGGMLITAAPLV
                     DIAPLERATMPGRVVVQYDKRDIETIKLIKLDLLGLRMLSSIDDALRDIATDCAVHVD
                     LDRLPEDIPEVFRMIQAADTVGVFQIESRAQMQTLPRSRPTTLDDLVVEVAIIRPGPI
                     QGNAVHPYLRRRQGIEPVTYLHPSLQPALEETMGVILYQEQVMKIAIDVAGFSAGESD
                     GFRRAMGTWRSSREMEKLHERFVNGCITRNGLTAEQAEELFRQVSAFASFGFNKSHAA
                     AFARTAYESAFLKLFYPAQFVTGLINAQPMGFYPIEVLVNDAKRHGVAVLPVDVNRSR
                     YLTRTEWVGMPDEPLPDGAGIDHRPVAVQSSGCVVPDQQARDRWAAPTAIGYGIRLGL
                     GLVKGIGEAEGEALDAEVARGGPFQSLADLAARTELAEEVLARLIRAGALDSFNEPRR
                     KLLWQLREITGATRGRYDGRSVGSKKAQGRPLDLRLPPTDAPDLPPPTELERLGDAYA
                     ILSLDARRQVIELFRPALDSLGVTTAGALVDTRPGRVKIGGLVVTRQHPMTAKGTVFL
                     ALEDETGMVNVTLWPDTWAAFRATVRRHALLYVEGRLERQSDVVNVIADRVESLAAVA
                     RPAGGPNQPQGVRHMGHAGMRRVG"
     gene            complement(2324..3007)
                     /locus_tag="VIF08_00480"
     CDS             complement(2324..3007)
                     /locus_tag="VIF08_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134486.1"
                     /translation="MARAKNTSRAEARKRTREVQRAELAEDGQLDDALDDVSEDAQPA
                     RRPSMFQMPNVLEDIRALPSIFRSRRLIWLPFILVAIGFLLALVIYGVPVEFQPWVAL
                     YLQFFFVPQGLFAYFIAGFVAPRASYLLGGMLGLMSGIMYGTALIVTLPPDTDAVVRA
                     SVESVALSSPVMGGILGTFAGGFAAWYRNFLRGMQQSGAQRRADREARERAKRREERQ
                     EARKVFKGR"
     gene            3186..>4266
                     /locus_tag="VIF08_00485"
     CDS             3186..>4266
                     /locus_tag="VIF08_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEY8134487.1"
                     /translation="MAVRARVPRIIWLAGLVAAFCLAWAPVGASAVAWPASAGLLVSE
                     VMTGGASASDEFVELYNASPIEVDLGGLELVYVTSSGSTVTRKQTWSALALGAHRHLL
                     VANSAGLYAASADGTYSGGLSGAGGSIAVRTLGGTVIDSLSWGDAASALVEARPGSAP
                     PAGSSLERRPGAALGNGTDTNDNLADTLLNAAPIAQGLAAPPVPALVATPSPTSTPVP
                     TPICEWAAPTPTATPWPFPTSVASPTPAASPTPGPTGPIVTLTGQLITATGLIDDGRT
                     AYLDLGAAGVALRLESADWPALVAGTYVSATGVLGERHGEATLHLGSAVDLVYLGEGS
                     RPAPLRAATGLACEAFESRLIAVEGV"
BASE COUNT          666 a         1458 c         1489 g          653 t
ORIGIN      
        1 tccgatgacg agggcgggcc gggcgacacg ccggtcagcg tgcgctggct gggcgccgag
       61 gatcgggccg cggaggtgca tccggtgctg caacgcccgc cgcgccgcat cgaccccggg
      121 gaagcgttcg agtccgcgct gtcgagcggc ccaccagcac tattactagg aaagcgacaa
      181 acagttgggg tgacgatttc tgagggggcg tcggcgggca attcggagca gtccgacgct
      241 cagcgggctg gtttggtggc catccatcgc ggggtaaacg cgcccactaa ccatagtgcg
      301 ggtacggagg ctgtcaggcc gggcgaggac acatctgcgg gcgtgtctct cgggtcgacg
      361 gcagggctga cgccgtggga caggtggctg gagctgaccg cgcagatcga tggcttcccg
      421 cgacacctgt cgatccacac cggtgggatg ctgatcacgg ccgctcccct ggtagacatc
      481 gcgccccttg agcgggcgac gatgccgggc cgtgtcgtgg tgcaatacga caaacgcgac
      541 atcgagacga tcaagctgat caaactcgac ctgctcggcc tccggatgct gtcgagcatc
      601 gatgacgcgc tcagagatat cgcgacagac tgcgcggtcc acgttgacct tgaccggctg
      661 ccggaggaca tcccggaagt cttccgcatg atccaggccg cggacacggt gggcgtcttc
      721 cagatcgaga gccgcgccca gatgcagacg ctcccaagat cgcgcccgac gacgctcgac
      781 gatctcgtag tggaggtggc gatcatccgc cccggaccca tccagggcaa cgcggtccac
      841 ccctacctgc gacggcgcca gggcatcgag cccgtgactt acctgcatcc cagcctgcag
      901 cccgcgctcg aggagacgat gggcgtgatc ctctaccagg agcaggtcat gaagatcgcg
      961 atcgacgtcg ccggcttcag cgcgggtgag tcggacggct tccgccgggc catgggcacc
     1021 tggcgttcca gccgcgagat ggagaagctc cacgaacgct tcgtgaacgg ctgcatcacc
     1081 cgcaacggcc tgaccgccga acaggcggag gagctgttcc gccaggtatc ggcgttcgcc
     1141 agcttcggct tcaacaagag ccacgcggcc gcgttcgccc ggaccgccta cgaaagcgcg
     1201 ttcctgaagc tgttctatcc cgcacagttc gttaccgggc tcatcaacgc ccagcccatg
     1261 ggcttctacc cgatcgaggt cctggtgaat gacgccaagc gccacggggt ggccgtccta
     1321 ccggtcgatg tcaacaggag ccgctacctc acgcggacgg agtgggtcgg gatgcccgac
     1381 gagccgctcc ccgacggcgc cggcatcgac caccggccgg ttgcggtcca atcaagtggc
     1441 tgcgtggtgc ccgaccagca ggcacgcgac cgctgggccg cgccgaccgc gatcggctac
     1501 ggcatccgac tggggttggg cctcgtcaag ggcatcggcg aggccgaggg ggaggcgctc
     1561 gacgccgagg tggcgcgcgg cggtcccttc cagtcgctgg ccgacctcgc ggcgcggaca
     1621 gagcttgccg aggaggtcct ggcacggctg atccgggccg gcgcccttga ctcgttcaat
     1681 gagccgcggc gcaagctgct ctggcaattg cgcgagatca ccggcgcgac gcgcggccgg
     1741 tacgacggcc ggagtgtcgg ttcgaagaag gcccaaggcc ggccactcga tctccgcttg
     1801 ccaccgaccg atgcgcccga cctaccgcca ccgaccgagc tcgaacgcct tggtgatgcc
     1861 tatgcaatcc tgtcgctcga cgcgcgccgg caggtcatcg agctgttccg gccggcgctc
     1921 gactcacttg gtgtgaccac cgccggcgcg ctggtcgaca cgcggccggg tcgggtgaag
     1981 atcggcgggc tcgtcgtaac gcgccagcac cccatgactg ccaagggcac cgttttcctg
     2041 gcgctcgagg acgagaccgg catggtcaac gtcactctct ggccggatac ctgggcagcc
     2101 ttccgggcta ccgtgcgccg gcacgccctg ctgtacgtgg agggccgcct ggaacgccag
     2161 tcggacgtgg tcaacgtgat cgccgaccgg gtcgagtcac tcgccgcggt cgcccgaccg
     2221 gccggcgggc ccaatcagcc gcagggcgtc aggcacatgg gccacgccgg catgcgccgc
     2281 gtgggctgag ccttggcgcc cgccggggga gcaacctgtg gcgctagcgc cccttgaaga
     2341 ccttgcgcgc ttcctggcgc tcctcgcgcc gcttggcgcg ctcgcgcgcc tcgcgatcgg
     2401 cccgtcgctg cgcgccgctc tgctgcatgc cccgcaagaa gttgcggtac cacgccgcga
     2461 agccgccggc gaatgtgccc aggatgccgc ccatgaccgg gctcgacagg gccactgact
     2521 cgacacttgc tcgcactacg gcatctgtgt cgggaggcag ggtcactatg agggcggtgc
     2581 cgtacatgat tccgctcatg aggcccagca tcccgcccag caggtatgac gcgcgcggtg
     2641 ccacgaagcc cgcgatgaag taggcaaaga ggccctgtgg cacgaagaag aactgcaggt
     2701 agagcgcgac ccacggctgg aactcgactg gtacgccgta gatgacgagc gccaggagga
     2761 agccaatcgc gaccaggatg aacggcagcc agatcagcct gcgactgcga aagatgctcg
     2821 gcagcgcgcg gatgtcctcg aggacgttgg gcatctggaa catgctcggc cggcgtgctg
     2881 gctgagcgtc ctcggaaacg tcgtccaggg cgtcgtcgag ctgcccgtcc tcggccagct
     2941 cggcgcgttg gacctctcgg gtgcgcttgc gggcctcggc gcgtgacgtg ttcttggcgc
     3001 gggccaagtc ggatctcctt caggaaaggg gtgggggtcg gatgctagcg catggggtgt
     3061 ggaacaccgg gtaatcggcc gataaggcca accgacgaag atcttcccac cgcctccgcc
     3121 gcctccgccg cagcatccgc cggcgtaaac gtgtcgcgtc ctgccgccgc ggaggttctt
     3181 cgttcgtggc tgttcgagcg cgtgttcctc ggatcatctg gctggccggg ttggtcgcgg
     3241 cgttctgcct ggcatgggcg cccgtcgggg cgtcggccgt cgcctggcca gcgtcggcgg
     3301 gtctgctcgt aagcgaggtg atgaccggcg gtgcgagtgc gtcggacgaa ttcgtcgagc
     3361 tctacaacgc atcacccatc gaagtggacc tgggcgggct ggagcttgtt tacgtgacgt
     3421 cgagcggctc gacggtgacg cgcaagcaga cctggtcggc gctggccctg ggcgcgcatc
     3481 gccacttgtt ggtcgcgaac tcggccgggc tgtacgcggc cagcgccgac gggacgtaca
     3541 gcggcggact ctctggcgcg ggcgggtcga tcgccgtgcg cacgcttggc gggacggtga
     3601 tcgactcact cagctggggt gacgcggcgt cggcccttgt cgaggcgcgt ccagggtcag
     3661 cgccgcccgc aggctcaagc ctcgagcggc gacctggcgc cgcgctgggc aacggcacgg
     3721 acactaacga caacctcgcc gacacgttgc tcaacgccgc gccaattgcg cagggattgg
     3781 ccgcgcctcc ggtgccggcg ctcgtggcca cacccagccc gacctccacg ccggtgccga
     3841 ctcctatctg cgagtgggcg gcgcccaccc caacggccac gccgtggcca ttcccgacgt
     3901 cggtcgcttc gccgaccccg gccgcctcgc cgacgccagg cccgaccgga cccatcgtca
     3961 cgttgaccgg ccagctcatc accgcaacgg gcctgatcga cgacggacga accgcgtacc
     4021 tcgacctggg cgcggccggc gtcgcgctac gcctcgagtc ggccgactgg ccggcgttgg
     4081 tggccggcac ctacgtctcg gcgacggggg tcctgggcga gcgccacggt gaggcgacgc
     4141 tccacctggg tagcgcggtc gatctcgtct acctgggcga aggttcgcgg ccagcaccgc
     4201 tgagggcggc gaccggcctc gcctgcgagg cattcgagtc gcggctcatc gcggtcgagg
     4261 gcgtta
//
LOCUS       DATBRX010000013         4367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3022264, whole genome shotgun sequence.
ACCESSION   DATBRX010000013 DATBRX010000000
VERSION     DATBRX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3022264"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2582)
                     /locus_tag="VIF08_00490"
     CDS             complement(<1..2582)
                     /locus_tag="VIF08_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY8134488.1"
                     /translation="MSVESRTPSPFAPVARTLDLPALEHDVLRLWDERQTFARLREQN
                     AGHERWSFLDGPITANNPMGVHHAWGRTYKDMFQRFHAMLGHDQRYQNGFDCQGLWVE
                     VNVERELGFTSKRDIESFGIAKFINLCKQRVLTYAALQTEQSQRLGMWMDWNDPAELR
                     RLHDLLGEDPSQRVTVQGPDGSVTDSVEMVVGRLGLPELGGSYFTFSNENNYLIWQFL
                     AECNKRGWIYKGHDSMPWCTRCGTGISQMEMNEGYQDREDPGLTVRLPLVERPGEYLL
                     VWTTTPWTLAANVAAAVGPALAYVKVRQGEDEYWLGKGTLKTALKGDFKVLEEQPGTH
                     LVGWRYRGPFDELPYVQSAFAEGKGDEGGAGDFSDPVHRVVAWTDVTEAEGTGIVHIA
                     PGAGAEDYRLGKDLGLPVIAPLDEFGHYLDGFGWLSGLDAHAVAERVVEDLQGKGVFY
                     HLEPYSHRYPHCWRCGTALLFRVVDEWYISMGAVYDKPRDQVTAAEKERSLRYQIMDV
                     VDDIRWLPGFGYERELDWLRTMADWMISKKRYWGLSLPIWECASCGHFDVVGSRDELQ
                     QRALAGWQDFDGHTPHRPYVDAVKIACSACGAEISRIPDVGNPWLDAGIVPFSTMHYR
                     VDPDNWAKWFPADFITESFPGQFRNWFYSMLAMSTVLRREAPFKTILGYATLFGEDGR
                     PMHKSWGNSIEFNEAAERMGVDVMRWMYAKARPEENILFGYHTADEVRRELLVLWNVY
                     VFFVTYARLAAWTPASSDSAGDPVGSGVLDRWILSRSHGLARDVGARLADFDSTGATR
                     LVSTYIDDLSTWYLRRSRSRMRSTASAAEREAAFATLHLALTTASRVLAPILPFMSEA
                     MYQNL"
     gene            complement(2627..3601)
                     /locus_tag="VIF08_00495"
     CDS             complement(2627..3601)
                     /locus_tag="VIF08_00495"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY8134489.1"
                     /translation="MLTQKPARSPLPGTGARPADDSIVAYFQGKFVPLSEARLPVMTH
                     GFLYGTATFEGIRAYWNEEQEQLYGLKLLEHYKRMERSAKVLLMKLPMPPEELVALTV
                     ELLRKNGFRQDAYIRPTLYKSSEIIGVRLHNLEQDLLIFTVPFGEYIAIDRGISAQVV
                     SWRRNSDQSLPARAKITGAYVNSAFSKSEAVLNGYDEAIVLTIDGSVSEGSAENLFIV
                     RDGKLITPAISDDILEGITRSGLMQLAREEMGLETVVRTVDRSELYSCDEAFLCGTGA
                     QLSPIVDIDHRQVGNGEVGPITRQLAKLYFDTVRGRMPAYSDWVTPIY"
     gene            complement(3674..>4367)
                     /locus_tag="VIF08_00500"
     CDS             complement(3674..>4367)
                     /locus_tag="VIF08_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134490.1"
                     /translation="VAYAIAVGAALIPSEMAYLRYGAVIALIGVYAWYVKSHFEADPD
                     VDAADLTPLRFRRVDVRHHRTVPESPRLRIVTLQVLVALAMIIGGAVIFVDAVEHIAT
                     DLQLPAILISLVVAPIATELPEKFNSLIWVRNGKDTLAMGNITGAMVFQSCIPTTIGV
                     LLAADAWSLAGASTETLISFASAGIAFASTLLIFWPMVRRGSLSGRHLLIGGIFYALY
                     LALVIGRLTGLL"
BASE COUNT          742 a         1452 c         1462 g          711 t
ORIGIN      
        1 aggttctggt acatcgcctc cgacatgaag ggcaggatgg gcgcgagcac gcggctggcc
       61 gttgtgagcg ccaggtgcag cgtcgcgaac gccgcttccc gctccgcggc gctcgccgtc
      121 gaacgcatgc gcgagcgcga acggcgcagg taccacgtcg acagatcgtc gatgtaggtg
      181 ctgacaagcc gcgtcgcacc ggttgagtcg aagtccgcca ggcgcgcccc aacgtcgcgc
      241 gccagcccat gggagcgcga caggatccag cgatcgagca cgcctgaccc aaccgggtcg
      301 cccgccgagt cggagctggc aggcgtccag gcggcgagtc gggcgtaggt gacgaagaag
      361 acgtagacgt tccacagcac gagcagctcg cgccgcacct cgtccgcggt gtggtagcca
      421 aagaggatgt tctcctccgg ccgcgccttg gcgtacatcc agcgcatcac gtcgacgccc
      481 atgcgctcgg ctgcctcgtt gaactcgatc gagttgcccc agctcttgtg catgggccgc
      541 ccgtcctcac cgaaaagcgt ggcgtagccg aggatcgtct tgaagggcgc ctcgcggcgc
      601 agcacggtgc tcatggcgag catcgagtag aaccagttgc ggaactggcc ggggaacgac
      661 tcggtgatga agtcggccgg gaaccacttg gcccagttgt cgggatcgac gcggtagtgc
      721 atcgtcgaga acggcacgat gcccgcgtcc agccagggat tgcccacgtc tgggatgcgc
      781 gagatctcag cgccgcacgc cgaacaggcg atcttcaccg cgtcgacgta cggccggtgc
      841 ggcgtgtggc cgtcgaagtc ctgccacccg gcaagggcgc gctgctgcag ctcgtcgcgg
      901 ctgccgacca cgtcgaagtg gccgcacgac gcgcactccc agatgggcag cgacaggccc
      961 cagtagcgct tcttggagat catccagtcg gccatcgtgc gcagccagtc cagctcgcgc
     1021 tcgtagccga agcccggcaa ccaccgaatg tcgtcgacga cgtccatgat ctggtagcgc
     1081 aggctgcgct ccttctcagc ggccgtcacc tggtcacgcg gcttgtcgta gaccgcgccc
     1141 atcgagatgt accactcgtc gacgacgcgg aagagcaacg ccgtgccgca gcgccagcag
     1201 tgcgggtagc gatggctgta cggctcgagg tggtagaaga cgcccttgcc ctgcaagtcc
     1261 tcgacgacgc gctccgccac cgcgtgcgcg tccaaacctg atagccagcc gaagccgtcg
     1321 aggtagtgcc cgaactcgtc gagcggtgca atgaccggca gccccaggtc cttgcccagg
     1381 cgatagtcct cggcgccggc gccgggcgcg atgtgaacga tgcccgtgcc ctcggcctcg
     1441 gtcacgtcgg tccacgcgac gacgcggtgc actggatcag agaagtcacc ggcgccaccc
     1501 tcgtcgccct tgccctcagc gaatgccgac tgcacgtatg gcagctcgtc gaacggcccg
     1561 cgatagcgcc agccgaccag gtgtgttccg ggctgctcct cgagcacctt gaaatcgccc
     1621 ttgagggcgg tcttgagcgt gcccttgccg agccagtact cgtcctcgcc ctggcgcacc
     1681 ttgacgtagg cgagcgccgg ccccacggcc gcggcgacgt tcgcggcgag cgtccacggg
     1741 gtcgtcgtcc agaccagcag gtactcccct ggccgctcga ccagcggcag gcgcacggtc
     1801 agtcccggat cctcgcggtc ctggtagccc tcgttcatct ccatctggct gatgccggtg
     1861 ccgcagcgcg tgcaccacgg catggagtcg tggcccttgt agatccagcc gcgcttgttg
     1921 cactcggcca ggaactgcca gatgaggtag ttgttctcgt tgctgaaggt gaagtacgac
     1981 ccacccagct cgggcaggcc aagccggccg accaccatct cgaccgaatc ggtcacggat
     2041 ccgtccgggc cctggacggt tacccgctgc gatggatcct cgcccagcag atcgtgcaga
     2101 cgccgcagct cggccgggtc attccaatcc atccacatgc ccaggcgctg cgactgctct
     2161 gtctggagcg cggcgtacgt caggacgcgc tgcttgcaca ggttgatgaa cttggcgatg
     2221 ccgaagctct cgatgtcgcg cttgctcgtg aaaccgagct cgcgctcgac gttgacctcc
     2281 acccacaggc cctggcaatc gaagccgttc tggtagcgct ggtcatggcc gagcatcgcg
     2341 tggaagcgct ggaacatgtc cttgtaggtg cgcccccagg cgtggtggac gcccatcgga
     2401 ttgttggccg tgatcgggcc gtcgaggaac gaccagcgtt cgtgtccggc gttctgctcc
     2461 ctcagccgcg cgaacgtctg gcgctcgtcc cagaggcgca gcacgtcatg ctcgagcgcg
     2521 ggcaggtcaa gcgtgcgcgc gacaggcgca aacgggctcg gggtgcgcga ctcgaccgac
     2581 atgggacgtt caagtctagc cgcgcgaccc cctcgcggct ggggccctag tagatgggcg
     2641 tgacccagtc ggagtaggcg ggcatcctgc cgcgaacggt gtcgaagtag agcttggcca
     2701 gctggcgcgt gatcgggccg acttcaccat tgcccacctg gcgatggtcg atgtcgacga
     2761 tgggcgagag ctgcgcaccg gtgccgcaca gaaacgcctc gtcgcacgag tagagctcgc
     2821 tgcgatcgac cgtgcgcacc accgtctcga ggcccatctc ctcgcgcgcg agctgcatca
     2881 gcccggagcg cgtgatgccc tcgaggatgt cgtcgctgat ggccggcgtg atgagcttgc
     2941 cgtcgcgcac gatgaagagg ttctccgccg atccttccga cacggagccg tcgatggtca
     3001 gcacgatcgc ctcgtcgtag ccgttgagga cggcctcgct cttggagaac gcgctgttca
     3061 cgtacgcgcc ggtgatcttg gcgcgcgccg gcagcgactg gtcgctgttg cgacgccacg
     3121 agacgacctg cgcgctgatg ccgcggtcga tggcgatgta ctcaccgaat ggcaccgtga
     3181 agatcagcag gtcctgctcg aggttgtgca ggcggacccc aatgatctcg ctcgacttgt
     3241 acagcgtggg ccggatgtac gcgtcctggc ggaagccatt cttgcgcagc agctcgaccg
     3301 tcagggcaac gagctcctcg ggcggcatcg gcagtttcat gagcagcacc ttggccgagc
     3361 gctccatgcg cttgtagtgc tcgagcagct tgagcccgta gagctgctcc tgctcctcgt
     3421 tccaataggc gcggatgccc tcgaacgtgg cagtgccgta caggaagccg tgcgtcatca
     3481 cgggcaggcg cgcctcagag agaggaacga acttgccctg gaagtaggcg acgatggagt
     3541 cgtcggctgg ccgggcgcca gtcccgggta gcggcgacct ggcgggtttc tgggtcagca
     3601 tcggcgcgga gtatacccct gccccgagtg catcagatga gcccttggca cccattgatt
     3661 cgggccaaat gccttacagc agccctgtca ggcggccgat gacaagcgcc aggtagagcg
     3721 cgtagaagat tccgccaatg agcaggtgac ggccgctgag cgagccgcgc cgcaccattg
     3781 gccagaagat gagcagcgtg gacgcgaacg cgatcccggc cgacgcgaag ctgatgagcg
     3841 tctcggttga cgcgccggcg agcgaccagg catccgcggc cagcagcacc ccgatcgtcg
     3901 tcgggatgca cgactggaag accatggccc cggtgatgtt gcccatcgcc agcgtgtcct
     3961 tgccgttgcg cacccagatc aggctgttga acttctcggg cagctcggtc gcgatgggcg
     4021 ccacgacgag cgagatgagg atcgccggca gctgaagatc cgtggcgatg tgctcgaccg
     4081 cgtccacgaa tatgaccgcg ccgccgatga tcatcgccag cgccaccagg acctgcagcg
     4141 tgacgatccg caggcgcggc gactccggca cggtcctgtg gtggcgcacg tcgacgcgcc
     4201 ggaagcgcag tggcgtgagg tcggcggcgt cgacgtccgg atcggcctcg aagtggctct
     4261 tgacgtacca ggcatacacg ccgatcaggg cgatgaccgc gccgtaacgc aggtaggcca
     4321 tctcactcgg gatcagagcg gcaccaacgg cgattgcgta ggcaacg
//
LOCUS       DATBRX010000014         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1011854, whole genome shotgun sequence.
ACCESSION   DATBRX010000014 DATBRX010000000
VERSION     DATBRX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011854"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1142
                     /locus_tag="VIF08_00505"
     CDS             231..1142
                     /locus_tag="VIF08_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8134491.1"
                     /translation="MKRELLPSAVDPTVFDEAFLRQLERLMLVTRKAVRGGMKGVRRS
                     VKRGQSVEFTDYRDYSLGDDLRSLDWNLYARLEKLFIKLFIEEEDVNVHILVDASASM
                     EGGAPDKLLFAKRAAAALAYVGLASYDRISMAVLQGRVARRFPPVRGTGRVFQVLADL
                     SSVQPAPGPTDLVSACRHYAAQITQRGPLMLISDLFDANVERAISELAGTRCDVAILH
                     TLSTDELDPPLEGDLRLVDRETGDGVNVTADLYTIDAYKSRLAAWQARLEEVSTKRRV
                     SYVPIPTTLPLADLVFAELRRRNVVAA"
     gene            1146..>2454
                     /locus_tag="VIF08_00510"
     CDS             1146..>2454
                     /locus_tag="VIF08_00510"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8134492.1"
                     /translation="MGLLAPLALLGLIAIPIIVAFYMLRLRRPTQPVSSTFLWQQLVR
                     DVEANAPWQKLKRSLLLLLQLLLALLLAFVVARPFSEHPAGLARDLVLVIDASASMSA
                     TDVFPDRLTAAKRLAQEQLVQVPSDGRVSVIAAGDTARVVANEATDRGRISQAIESVE
                     ASTSASDLTDALKLANSLAKRARGAEVLVVTDDAFSASPDVALEVPVKVLTVGRERDN
                     QAIAALAVRADPSGLKRTLFVSIANYSASRVGRSLQILADGVSVTARDLYLEPLSRAD
                     IVIDELPAGSAVVEARLAPATDDDAPVGPPDYLPLDDAAWAIVPPDSLRKVLLVGPGN
                     VYIQNAFALLPNVELYGATAAQWPTTTGKDKFDLIVFDGFVPPDLPNKPILAFAPPAT
                     SPLGVVLGTLDAFGMGQPAVNDPLLRGVDLTRLHIAKTQQMELP"
BASE COUNT          372 a          906 c          780 g          396 t
ORIGIN      
        1 ccctggcgac ccttgtctcg tcgcgtgaca ccactatggt caccgcggcg cttaaggggc
       61 actgaggcac cccttcaatg caccctttca acgccgacct gggcaagcca cgctcaccgg
      121 agctcacacg gtgcccgatc agcggcgcga tgggcctcgc tccaggcaat tagggctcgt
      181 tttgaccata gcagcgtcac gccagcggcc aagccgcgag gcagccggcg atgaagcgcg
      241 agctgctgcc gtcggccgtt gaccccacgg tcttcgacga ggcgttcctg cgccagctcg
      301 agcggctgat gctggtcacg cgcaaggccg tgcgcggcgg catgaagggc gtgcgccgca
      361 gcgtcaagcg cggccagagc gtcgagttca ccgactaccg cgactactcg ctcggcgacg
      421 acctgcgctc gctcgactgg aacctgtacg cgcggctcga gaagctcttc atcaagctgt
      481 tcatcgagga agaggacgtc aacgtccaca tcctcgtgga cgcgtccgcc agcatggagg
      541 gcggcgcgcc ggacaagctg ctgttcgcga agcgcgccgc cgcagcgctg gcctatgtgg
      601 gcctcgcgtc atatgaccgg atcagcatgg ccgtcttgca gggccgcgtg gcacggcgct
      661 tcccacccgt gcgtggcacg ggccgcgtct tccaggtgct ggccgacctg agcagcgtgc
      721 agcccgcgcc cggcccaact gaccttgtct ccgcgtgccg ccactacgcg gcccagatca
      781 cgcagcgcgg cccgctgatg ctgatcagcg acctctttga cgcgaacgtc gagcgggcga
      841 tctccgagct tgccggcacg cgctgtgacg tcgccatcct gcacacgctc tcgaccgatg
      901 agctcgatcc gccgctcgag ggcgacctac gcctcgtcga ccgcgagact ggcgacggcg
      961 tcaacgtgac cgccgacctg tacacgatcg atgcctacaa gagccgcctc gcggcctggc
     1021 aggcgcgcct cgaggaggtc agcaccaagc gccgcgtctc gtacgtgcca attcccacga
     1081 ccctgccact cgcggacctg gtcttcgccg agctccgccg gcgcaacgtg gtcgccgcct
     1141 gagccatggg tctgctcgca ccgctggcgc tgctcggcct catcgcgatc ccgatcatcg
     1201 tcgccttcta catgctgcgc ctgcgccggc caacgcagcc cgtcagcagc acgttcctct
     1261 ggcaacagct cgtaagagat gtcgaggcca acgcgccgtg gcagaagctc aagcgctcgc
     1321 tgcttctcct gctgcagctg ctgctcgcgc tgctgctcgc gttcgtcgtc gcgcggccgt
     1381 tcagcgagca tcccgcgggc ctcgcccgtg acctcgtcct ggtcatcgac gcgtcggcat
     1441 cgatgtccgc cacggacgtc ttccccgacc gactgaccgc cgccaagcgc ctcgcccagg
     1501 aacagctcgt ccaggtccca tcggacggtc gggtgagcgt gatcgcggca ggcgataccg
     1561 cccgcgtggt ggccaacgag gcgacagatc ggggccgcat cagccaggcc atcgaatcgg
     1621 tcgaggcgtc gacatctgcg tccgatctga ccgacgcgct caagctggcc aactcgctgg
     1681 ccaagcgggc ccggggtgcc gaggtgctcg tggtgaccga cgacgccttc tcggcctcac
     1741 ctgacgtcgc cctcgaggtg cccgtcaagg tcctcactgt cggccgcgag cgcgacaacc
     1801 aggccatcgc cgcactggcc gttcgcgccg atccatcggg tctcaagcgc acgctcttcg
     1861 tgagcatcgc caactactcg gcgtcgcgcg tcggccgctc gctgcagatt ctcgcggacg
     1921 gcgtgtcggt cacggcgcgc gacctgtatc tcgagccgct cagccgggcg gacatcgtga
     1981 tcgacgagct gcccgcgggt tcagcggtcg tcgaggcgcg gctcgcgccc gccactgatg
     2041 acgacgcgcc ggtcggaccg cccgactacc tgccgctcga tgacgccgcg tgggcgatcg
     2101 tgccgcccga ttcactgcgc aaggtgctgc tcgtcgggcc gggcaacgtc tacatccaga
     2161 acgcgttcgc cctgctgccc aatgtcgagc tgtacggggc aacggctgcg caatggccga
     2221 ccaccactgg caaggacaag ttcgacctca tcgtcttcga cggcttcgtg ccgcccgacc
     2281 tgccgaacaa gccgatcctc gcgtttgctc ccccggcaac gagcccgctc ggtgtggtcc
     2341 tgggcacgct cgatgcgttc ggcatgggcc agcccgcggt caacgatccg ctgctgcgcg
     2401 gcgtcgacct gacgcggctg cacatcgcca agacgcagca gatggagctg cctg
//
LOCUS       DATBRX010000015         7833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2687096, whole genome shotgun sequence.
ACCESSION   DATBRX010000015 DATBRX010000000
VERSION     DATBRX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687096"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VIF08_00515"
     CDS             <1..615
                     /locus_tag="VIF08_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY8134493.1"
                     /translation="LSRGPEAVLYAILDRVVDGYAPVVAGLQKDIDEIEVEVYRGDPK
                     VSRRIYELSREVIDFQRSTRPLLAVLAGLEAGFEKYHTDEELQRYLRDVADHATTVVE
                     RVDGFRQILSDILTLNATLVNQAQNEEIKRLTEASFAQNEEVKRISAWAAIIFAPTLI
                     GTIYGMNFVSMPELQWAFGYPFALMLMVVVSVGLFLVFKARDWL"
     gene            612..2195
                     /locus_tag="VIF08_00520"
     CDS             612..2195
                     /locus_tag="VIF08_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8134494.1"
                     /translation="MSHSPRAGAWVLLGALGMGVLLVGIELMITAVALPSIIADLGGW
                     TRLREASWIVNGYLVAYVAVMPLAGRAADRYSLATLYGLSLGAFALGSLACGAAQNLE
                     WLIGARIVQGAGAGAIFPLATAAAGHLFEGHARARAIGVIGALSFVGMAAGPVVGAAV
                     LSLEVPAGVLTAPAWRWVFYLPAPLAVLSGLYLWAAAPAWPRQAGAGRLDVVGAVLFT
                     SGLALALVALTVIGAPNVPDPALLALAAAVLLVLAVVRFARAREPFIRLGLFRDRTFS
                     GAVLMSLLTGYSLATAIIGAAVFVDRVRYAGPAEQQLTLGALAGAMAIGALSAGFLVR
                     PLGVMIPSLAGLLAEIGGLLILARAEPSTDLATLCAALALFGLGFGVTVTARTTGALE
                     ALGRTAFGVGSAALTVARMIGMGTGLAALTVLGSNRIEALSVVLVDPVARDAVLPEAL
                     RGRPLEDYLVVDALERWASGQASSILAWTFVVAAAVTAVAILPTLAMRNRPGSHARAA
                     QDEDAGNVVEEASRAGLAI"
     gene            2155..3177
                     /locus_tag="VIF08_00525"
     CDS             2155..3177
                     /locus_tag="VIF08_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY8134495.1"
                     /translation="MSSRRRHERGSPSERHPAGDGKAWALAWSQAGGLRTLTDLEAIK
                     KAHADDQSRLWIDLISPDPPVLEALTKLLALHPLVTEDILERNQRAKIEQTGDALHLV
                     MFGLEYAGEVVPHELDIVLGPRYLLTSHDAGIDLRDAPFMRREPGAYLTEGPDFMLWS
                     VTDWLVDQYFPVFDKLGDEIDELEVEVVRKPGTWVLERLFTLRRDLLEIRHAVNPQRE
                     IFNQLTNRDLGLIKPERIVYFRDVYDHLIRLTDELDSYRELVSTTLDIYLSQINNNLS
                     DTMKRLTGVTVILATIGAIGGIFGMSEAGAAFNFTESWGFWLVAVSAVVLALLVAYYF
                     RRRGWV"
     gene            complement(3185..3994)
                     /locus_tag="VIF08_00530"
     CDS             complement(3185..3994)
                     /locus_tag="VIF08_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEY8134496.1"
                     /translation="MKVVIWLLDRFVPTVLMGSSAALLAVGVLSYAPSAFGQWQTPEP
                     TIGGDPLFETTGPDGGVAWASGDPNGSGDLPRATPSPVPSPQPATPIPIGPTPTPTST
                     PAGDAVVTRVLIPSLDIDLPVVAGDLEVRGNEGNYPLCDVAMYMIGFVNPGQPGTTYI
                     YGHAQAGMFAPLLHASKYNNGASMIGALVQVYTNDSMLHLYEIKLVKRHATDLSITRV
                     PAGVHQLVLQTSEGWHGHIPKLQVAAKPLTVLPVSYGDAHPTPHPRVCLPD"
     gene            4051..4692
                     /locus_tag="VIF08_00535"
     CDS             4051..4692
                     /locus_tag="VIF08_00535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8134497.1"
                     /translation="MRQPDYLGVIAALVVGGVLTVLLLAGTLGTPAPLPTRPAVTIPP
                     VPTQSGGGSLPSVLPTGTPNGPGIGTAIGEIAPNIQVTLTDGSLIDTADFGDLPIWIN
                     FMATWCPQCATELPMMDRFEAQLKDQMTVLVIDVGEDRSVVRDFMRALDVDLTVGLDH
                     DGSIQAQWAANALPVHYMLDADGVVRQIVYGGAPEETFIQAVTVLVPEFSATD"
     gene            4697..5581
                     /gene="otsB"
                     /locus_tag="VIF08_00540"
     CDS             4697..5581
                     /gene="otsB"
                     /locus_tag="VIF08_00540"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY8134498.1"
                     /translation="MTQAATRTRASLVVSDLRGAVEMAAQHLHGRPLLVVTDFDGTIA
                     RIVDDPWGASILPLGRRALRALAGMPRVHVVVLSGRTARDAAARVRVGGVTYLGNHGM
                     ERAFLRRGGRAERLKTEVDDAAHEAVHAAALLSDEVPKLITEPWLVVERKPPAVAFHF
                     RTAPDLAQAAHDVRAAVDKLEPAGILERFPGRRVLELRPYGAVAKGESLQALVERYAP
                     HAVFMLGDDVSDAMAFRALRELRAKGVTDGIAVAVKARPDNIIAEVIEAADVVLGSPA
                     DATRFLAALARRLEADSA"
     gene            complement(5630..7264)
                     /locus_tag="VIF08_00545"
     CDS             complement(5630..7264)
                     /locus_tag="VIF08_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY8134499.1"
                     /translation="MKPLRPVRAVPDGTGQLRDYLAALLGNDGRLIVASNRGPLSFSR
                     ARSGEWRAKRGSGGLVTALGEIGRLAPVTWVSAAMDREDRAAAEALNGPPSDAQAELL
                     RLIEHELPGQDIRLDMRPVAEDAWRAHYSQFANPFLWFTQHQLFTLHYEPTVDDELIK
                     AWRRGYSVINDSLAQAVLRQTYTTQGRPVVLLQDYHLYLAAGEIRAERPDALLLHFNH
                     IPWPAVDTWMMLPQGLRRGICEGLLANDIVGLQTDRYATNFLSSVGAFVRDAEVDPDG
                     RHVRWRGRTIWVRAYPISIQPDSLAAFARSPAVERRRAELRARLERVGNPRLIVRVDR
                     LEPSKNALRGFVAFENLLKRRPELRESVRFLAIQSVSRPDIPDYVQYAAAVREAVARV
                     NGLADPDEAPIWLLDGSDYDLAIAALSLADVVLVNPVVDGMNLVAKEAAIVSRAALVL
                     SETAGAAEQLASDALMVSAADVSGTAAMLERALDMPLAERLLRIRRLRASVREEDLRW
                     WLTRQLRDLAAWSKGRRPPSRALRDTTREFEPTLHD"
     gene            complement(7440..>7833)
                     /locus_tag="VIF08_00550"
     CDS             complement(7440..>7833)
                     /locus_tag="VIF08_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY8134500.1"
                     /translation="LTTFAGLGRRAPLLAALMLLFLLSLTGIPPLAGFWAKWYVIQAA
                     LQVGGWLTLLAVLTVLNAAVAAFYYLRVAVYMYMREPADSAPRVELGGQTRLGLGLAA
                     LGTVAIGLVPPVTIAVITLAQGAAQALL"
BASE COUNT         1140 a         2674 c         2746 g         1273 t
ORIGIN      
        1 ctgagccgcg gtcctgaggc ggtgctgtac gcgatcctcg accgcgtcgt cgacggctat
       61 gcgccggtcg tcgccggact gcagaaggac atcgacgaga ttgaggtcga ggtgtaccgc
      121 ggcgacccca aggtgtcgcg gcgcatctat gagctgtcgc gcgaggtgat cgacttccag
      181 cgctcgacgc gacccctgct ggccgtgctc gcgggtctcg aggcgggctt cgagaagtac
      241 cacaccgatg aggagctcca gcgctacctg cgcgacgtgg ccgaccacgc caccacggtc
      301 gtcgagcgcg ttgacggctt ccgccagatc ctgtcggaca tcctgacgct caacgcgaca
      361 ctggtgaacc aggcccagaa cgaggagatc aagcgcctga ccgaggcgag cttcgcccag
      421 aacgaggagg tgaaacgcat ctccgcctgg gccgccatca tcttcgcgcc cacgctcatc
      481 ggcacgatct atggcatgaa cttcgtcagc atgcccgagc tccaatgggc ctttggctat
      541 cccttcgcgc tgatgctcat ggtcgtcgtc agcgtgggcc tcttcctggt cttcaaggcg
      601 cgcgactggc tgtgagccac tccccgcgcg ccggcgcgtg ggtgcttctt ggcgcactcg
      661 gtatgggcgt gctgctcgtc ggcatcgagc tgatgatcac ggccgtcgcc ctgccgagca
      721 tcatcgcgga cttagggggt tggacgcggc tgcgcgaggc gtcgtggatc gtgaacggct
      781 acctggtggc gtacgtcgcg gtcatgccgc tggccggccg cgccgccgac cggtattcgc
      841 tcgccacgct gtacggcctg tcgctgggcg ccttcgccct cggctcgctg gcgtgcggcg
      901 cggcccagaa cctcgaatgg ctgatcggcg cgcgcatcgt gcagggcgcg ggcgcgggcg
      961 cgatcttccc gctcgcgacg gccgcggcgg gtcacctgtt cgagggccat gcccgcgccc
     1021 gcgccatcgg cgtgatcggc gcgctgtcct tcgtcggcat ggccgcagga ccggtggttg
     1081 gcgcggcggt gctgtcgctc gaggtgccgg cgggcgtact cacggcgccg gcctggcgct
     1141 gggtcttcta cctgccggcg ccgctggcgg tgctatccgg cctgtacctg tgggccgctg
     1201 cgccggcgtg gccgcgccag gccggcgccg ggcggcttga cgtggtcggc gcggttctgt
     1261 tcacctccgg gctcgccctg gcgttggtgg cgctgaccgt gatcggggcg cccaacgtgc
     1321 ccgacccggc gctgctcgcc cttgccgccg cggtgctgct cgtgctggcc gtggtgcgtt
     1381 ttgcccgtgc ccgcgagccg ttcatcaggt tgggcctgtt ccgcgaccga acgttcagcg
     1441 gcgccgtgct gatgagcctg ctgaccggct actcgctcgc aaccgcgatc atcggcgccg
     1501 cggtcttcgt cgaccgggtg cgctatgccg gcccggcgga gcagcagctg acgctcggcg
     1561 cgctggccgg ggcgatggcc atcggtgccc tgagcgccgg cttcctggtg cgcccgctgg
     1621 gcgtgatgat cccgagcctc gctgggctgc tggcggagat cggtgggttg ctgatcctcg
     1681 cgcgggccga gccgtcgacc gacctcgcga cgctgtgcgc ggccctggcg ctgttcgggc
     1741 tgggcttcgg cgtgaccgtc actgcgcgga cgaccggcgc gctcgaggcg ctgggccgca
     1801 cggcatttgg cgtgggctcg gccgcgctga ccgtggcgcg catgatcggc atgggcacgg
     1861 gccttgccgc gctgaccgtt ctcggctcga accggatcga ggcgctcagc gtcgtcctcg
     1921 tcgacccggt ggcgcgcgac gcggtgttgc cggaagcgct gcgcggccgg ccgctcgagg
     1981 actacctggt ggtcgatgcg cttgagcgct gggcgtccgg gcaggcctcg tcgatcctcg
     2041 cgtggacgtt cgttgtcgcc gccgccgtga cggcagtcgc gattctgcct acgctcgcca
     2101 tgcgcaaccg cccgggcagc cacgcccgcg ccgcgcagga tgaggacgcg ggcaatgtcg
     2161 tcgaggaggc gtcacgagcg gggctcgcca tctgagcgcc accccgcagg cgacggcaag
     2221 gcgtgggccc tggcgtggag ccaggccggc gggctgagga cgctgaccga tctcgaagct
     2281 atcaagaagg cccatgccga cgaccaatcg cggctgtgga tcgacctcat ctcacccgac
     2341 ccaccggtac tcgaggcgct gaccaagctg ctcgcgctac atccactggt gacggaagac
     2401 atcctcgaac gcaaccagcg tgccaagatc gagcagaccg gtgacgcgct gcacctcgtc
     2461 atgttcgggc tcgaatatgc gggcgaggtg gtgccccacg agctcgacat cgtgctcggg
     2521 ccgcgctatc tgctgacgtc gcacgatgca ggtattgacc tgcgcgacgc gccattcatg
     2581 cgccgcgagc caggtgccta cctaaccgaa ggtcccgact tcatgctctg gtcggtcacc
     2641 gactggctgg ttgaccagta cttccctgtc ttcgacaagc tgggcgacga gatcgatgag
     2701 cttgaggtcg aagtcgtgcg caagccgggc acgtgggtct tagagcgatt gttcacgttg
     2761 cgccgcgacc tgcttgagat ccgccacgcg gtcaatccac aacgcgagat cttcaatcaa
     2821 ctcacgaacc gcgatcttgg cctgatcaag ccggagcgga tcgtctactt ccgcgacgtg
     2881 tatgaccacc tgatccggtt gaccgacgag ctcgactcat atcgcgagct ggtcagcacg
     2941 acactcgaca tatacctgtc gcagatcaac aacaacctgt cagacacgat gaagcgcctc
     3001 accggggtga ccgtgatcct ggccaccata ggcgccattg gcggcatctt cggcatgagc
     3061 gaggcgggcg ccgcattcaa cttcacggag tcgtggggct tctggctggt cgccgtcagc
     3121 gccgtcgtcc tcgcgctgct cgtcgcctac tacttccgcc gccggggctg ggtctaactg
     3181 gcggtcagtc tggtaggcac acccgtggat ggggcgtcgg atgggcgtcg ccatagctca
     3241 ccggcagaac ggtcagcggc ttggccgcca cctgcagctt ggggatgtgg ccatgccagc
     3301 cctcgctcgt ctggagcacg agctggtgga caccggctgg gacgcgggtg atcgacagat
     3361 ccgtggcgtg gcgcttgacc agcttgatct cgtacaggtg gagcatgctg tcgttcgtgt
     3421 agacctggac caaggcgccg atcatggatg cgccgttgtt gtacttcgag gcgtgaagta
     3481 gcggcgcgaa catgcccgcc tgggcgtggc cgtagatgta tgtcgtgccg ggctggcccg
     3541 gattgacgaa gccgatcatg tacatcgcca cgtcgcacag cgggtaattg ccctcgttgc
     3601 cgcgcacctc caggtcgccc gctaccaccg gcaggtcgat gtcgagcgac gggatgagga
     3661 cacgcgtcac caccgcatcc ccggccggtg ttgatgtcgg tgtgggggtc ggccctatcg
     3721 ggatcggggt ggccggttgc gggctcggca cggggctggg cgtagcgcgc gggaggtcac
     3781 ccgagccgtt cgggtcgccg ctcgcccagg cgacaccgcc atccggaccg gtggtctcga
     3841 acaacgggtc gccgccgatt gtgggctccg gcgtctgcca ttggccaaac gccgacggcg
     3901 cgtacgagag cacgcccacc gcgagcagtg ccgcgctcga gcccatcagg acagtgggca
     3961 cgaagcgatc gagcagccag ataacgacct tcattggacg cccgatgcta cctctgccac
     4021 cttttccacg ccgtagtact ctcgcttttc atgcgccaac ccgactacct cggcgtcatc
     4081 gccgcgctcg tcgtcggcgg tgtgctcacg gtgctgctgc tggcgggcac gcttgggacg
     4141 ccagcaccac tgccgactcg gccagcggta acgatcccgc cggttccgac gcaatccggc
     4201 ggcggttcgt tgccgagcgt gttgcccacc ggtaccccaa acgggccggg aatcgggaca
     4261 gccataggcg agatcgcacc caacatccag gtgaccttga ccgatggcag tctgatcgac
     4321 acggccgact tcggcgacct cccgatctgg atcaacttca tggccacatg gtgtccgcag
     4381 tgcgccaccg agctgccgat gatggatcgc ttcgaggccc agctgaagga ccagatgaca
     4441 gtgctcgtga tcgatgtcgg cgaggatcga tcggtcgtgc gcgatttcat gcgcgcgcta
     4501 gatgtcgacc tcaccgtggg gcttgaccac gacggctcga tccaggcgca atgggcggca
     4561 aatgccctgc ctgtccatta catgctcgat gccgatggcg tggtccgcca gatcgtgtac
     4621 ggcggcgcgc cagaggaaac cttcatccag gccgtgaccg tgctcgtccc cgagttctcg
     4681 gccaccgact agcgcgatga cccaggcggc gacccggacg cgtgcgtcac tggtcgttag
     4741 cgacctgcgc ggtgctgttg agatggccgc gcaacacctc catgggcggc cgctgctcgt
     4801 cgtgaccgac ttcgacggca cgatcgcgcg catcgtcgac gacccgtggg gtgcgtcgat
     4861 cctgccgctg ggccggcgcg cgctgcgtgc gctggcgggc atgcccaggg tgcatgtggt
     4921 cgtgctgtca ggccgcacgg cgcgtgacgc ggccgcccgc gtgcgcgtcg gtggcgtcac
     4981 gtacctgggt aaccacggca tggagcgcgc attcctgcgc cgcggcggcc gcgcggagcg
     5041 gctgaagacg gaggtcgacg atgccgcgca cgaggcggtg catgccgccg cgctgctgtc
     5101 ggacgaggtc ccgaagctga tcacagaacc ctggctggtc gtggagcgca agccgcccgc
     5161 ggtcgcgttc cacttccgga ccgcgcccga tctcgcgcag gctgcgcacg acgttcgcgc
     5221 cgccgtcgac aagctcgaac cggccggcat cctggagcgc ttccccggcc ggcgcgtgct
     5281 ggaattgcgg ccgtacggcg cggtcgcgaa aggtgaatcg ctccaggcgc tcgtcgagcg
     5341 ctacgcgccg catgcggtgt tcatgctcgg tgacgatgtc agcgacgcga tggccttccg
     5401 agcgttgcgc gagctgcgtg ccaagggcgt gaccgacggc atcgccgtgg ccgtaaaggc
     5461 gcggcccgac aacatcatcg ccgaggtcat cgaggccgcg gacgttgtcc ttggctcgcc
     5521 cgcggatgcg acacggttcc tggccgcgct cgcgcgccgg ctggaggcgg acagcgccta
     5581 gccggccgcg cggctcagcc ggctgcgcgg cccagcctgc cccgctgctt tagtcgtgca
     5641 gcgtcggctc gaactcgcgc gtggtgtcgc gcaacgcgcg cgacggcggc cggcggccct
     5701 tggaccatgc ggcgaggtcg cgcagctggc gcgtcagcca ccagcgcagg tcctcctcgc
     5761 gcaccgacgc gcgcaggcgg cggatgcgca acaggcgctc ggcaagcggc atgtccaggg
     5821 cgcgctcgag catggccgcc gtgcccgaga cgtcggccgc gctgaccatc agcgcgtcgc
     5881 tcgccagctg ctcggccgcg ccggcggtct cgctcagcac caatgccgcg cggctgacga
     5941 tcgctgcttc cttggccacg aggttcatcc cgtccacgac cgggttgacg agcacgacat
     6001 cggccagcga tagcgcggcg atggccaggt cgtagtcgga gccgtccagc agccagatcg
     6061 gggcttcgtc gggatcggcc agcccgttca cccgggcgac tgcctcacgc acggcggcgg
     6121 cgtactggac gtagtcgggg atgtccggcc ggctgaccga ctggatggcc aggaagcgca
     6181 ccgactcacg cagctcgggc cggcgcttga gcaggttctc gaacgcgacg aagccgcgca
     6241 gtgcgttctt cgatggctcg aggcggtcca cgcgcacgat cagccgcggg ttaccgacgc
     6301 gctcgaggcg cgcgcgcaac tcggcgcgac ggcgttcgac cgctgggctg cgcgcgaacg
     6361 ctgccagcga atcgggctgg atcgagatgg ggtaggcgcg cacccagatc gtccggccgc
     6421 gccagcgcac gtggcgcccg tcggggtcga cctccgcgtc gcgcacgaac gcgccgacgc
     6481 tcgataggaa gttcgtcgcg tagcggtcgg tctggagccc gacgatgtcg ttcgccagca
     6541 agccctcgca gatgccgcgg cgcagtccct gggggagcat catccaggta tcgaccgctg
     6601 gccacgggat gtggttgaag tgcagcagca gggcgtcggg tcgctcggcg cgaatctcgc
     6661 ccgcggccag atacaggtgg tagtcctgga gaagcaccac cggacgaccc tgcgtggtgt
     6721 aggtctggcg gagcacggcc tgggcgagcg aatcgttgat gacgctgtag ccgcggcgcc
     6781 acgccttgat cagctcgtcg tccacggtcg gctcgtagtg gagcgtgaac agctggtgct
     6841 gcgtgaacca cagaaaggga ttggcgaact gggagtagtg cgcccgccag gcgtcctccg
     6901 caaccggccg catatcgagc cttatgtcct ggcccggcag ctcgtgctcg atgaggcgta
     6961 ggagctccgc ctgcgcatcg ctgggcggac cgttgagcgc ttccgcggcc gccctgtcct
     7021 cgcgatccat ggccgcgctg acccaggtca ccggcgcgag ccggccgatc tcgcccagcg
     7081 ccgtgaccag gccgcccgag ccgcgcttcg cccgccactc accgctgcgc gcccggctga
     7141 agctcaaagg cccgcggttg gacgcgacga tcagccgccc gtcgttgccg agcagggcag
     7201 ccaggtagtc gcgcagctgg ccggtcccgt ccggcacggc ccggaccggg cgcaagggtt
     7261 tcatctgcat cttggagccg gccaagccta acggctggtc cctacgggag tgcgcgaagc
     7321 ggggtttggc ccattcctac cgcatatcac cggtgcgtgc cgcgacggcc acagcggtcg
     7381 acgaaggggg tcttagcgct tgggtcggat gtgcaccacg tgcgcagacc gacgatgccc
     7441 tagaggagcg cctgcgccgc cccctgggcc agcgtgatga ccgcgatcgt caccggcggc
     7501 acaagtccga tcgcgaccgt gcccagcgcg gcgagcccca gtcccaagcg cgtctggccg
     7561 cccagctcga cccgcggcgc ggagtcggca ggctcgcgca tgtacatgta gaccgcgacg
     7621 cgcaggtagt agaacgcggc cacggcggca ttgagcacgg tcagcaccgc gagcagggtg
     7681 agccagccgc cgacctggag cgcggcctgg atgacgtacc acttggccca gaagccggcg
     7741 agcggcggga tgccggtcag cgacaacagg aacagcagca tcaacgccgc gagcaacggc
     7801 gcgcgccggc ccagtccggc gaatgtcgtg agc
//
LOCUS       DATBRX010000016        11448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1458401, whole genome shotgun sequence.
ACCESSION   DATBRX010000016 DATBRX010000000
VERSION     DATBRX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458401"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1843
                     /locus_tag="VIF08_00555"
     CDS             191..1843
                     /locus_tag="VIF08_00555"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY8134501.1"
                     /translation="MAKYVFVTGGVTSSLGKGITAASIGRLLKSRGLSVSIIKLDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLTQLSNVTTGRIYQAVIAKE
                     RRGDYLGGTVQVIPHITNEIKERVVRVARDGKADVVIVEVGGTVGDIESLPFLEAIRQ
                     LRKDVGRTNVMYVHVTLLPALSATGELKTKPTQHSVKELRGIGIQPDVIIARADQDVP
                     DDLREKIALFCDVAVEAVIPAPTAETIYEVPLQFHERGLDDLIVRELGLEEKAKPADL
                     TAWRDLVELIKKPKPQLEIALVGKYIELPDAYLSVSEAMRHAGWAHGRSIVIRWVDSE
                     ALTADNYESVLSGAAGVLVPGGFGHRGIEGKVLAARYAREHKVPYLGLCLGLQCGVIE
                     FARDVVGAKDANSTEFDLFTNAPVIDFMPDQRDMEEKGGTMRLGLYPAKLTPGSKAAA
                     AYGEEVIYERHRHRFEVSNQYREALEKGGMILSGQSPDGRLVEIVELRDHPWFVASQF
                     HPEFRSRPDRPHPLFDGFVQAAIDQATRTGIPITTDAVPATA"
     gene            1861..2805
                     /locus_tag="VIF08_00560"
     CDS             1861..2805
                     /locus_tag="VIF08_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8134502.1"
                     /translation="MTAVLDPFVLERAPGPVVGLHPALVRLRDAFNGTLDVLGRIPDE
                     RLTSLWAWDGNDINVRYAFYRMLETLESGAAQVALAMRGQESSEAREAVGATSAARWD
                     LHGVLAALTDADLDADPGGGEWTIRQTMAHIIGGQRGYAWGSAYWISVRDEPKPEGPR
                     RAPDHLFASMPEDEEEATGTLAEVRQKLDDIVDAASSRFATLSTDEMDSAGGWYGFPV
                     TVSFRMWRWPSHIEEHTVQVEKTLDMLSHRATEVERLVRLNARALGRLEALVFGRAPE
                     QLDAAGVGATLDRLAGEIQQMAEPVKAAADAGVPGPPD"
     gene            complement(2816..3781)
                     /locus_tag="VIF08_00565"
     CDS             complement(2816..3781)
                     /locus_tag="VIF08_00565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8134503.1"
                     /translation="MTRYEVRSFGPADLDAAAELLADRHRRHRLAWPALNPAYEAPDA
                     TRPLVAALLEREGASGSITLADGEPVAYVVGAPRDAKWGANVWVEDAGSAGSDPEAIR
                     VAYADAAARWAAAGLTHHYAVTPATDARTVDAWFSLSFGLQHVHALRELPPADFVVRA
                     GALVVRPAEKRDIDAIVALEPILPTHSGQSPVFSSVLRSTPEAARADVEEEIEDPRLV
                     PYVAEAQGRVVGTATFCSLDMSDGNTPLMRPVSAGFLAYAAVARDARGLGAGRALGDA
                     CLVWARDNGHEWVAVDWRSTNLEADRTWRAAGFRPTFYRLFRAID"
     gene            3850..4539
                     /gene="fsa"
                     /locus_tag="VIF08_00570"
     CDS             3850..4539
                     /gene="fsa"
                     /locus_tag="VIF08_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEY8134504.1"
                     /translation="MKIFLDTADIEEIRTAARWGVLDGVTTNPSLFAKTSGKTYDEVL
                     VEICGITSGPVSAEVVAEDIEGMLKEGRHFAKLAPNIVVKVPMSEEGLEAIYRFAEEG
                     IKTNCTLIFTANQGLLAAKAGASLLSPFVGRLDDINQDGMDVIRELVTIVEVHDLDTE
                     VLAASIRHPRHMTDAALLGAHIATTPFKVLQQMVHHPLTDKGIKTFREDWEKARKAAE
                     ANAKSEASRHN"
     gene            complement(4553..5761)
                     /locus_tag="VIF08_00575"
     CDS             complement(4553..5761)
                     /locus_tag="VIF08_00575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8134505.1"
                     /translation="MADAPTRTLRTTRGRYQPREELGHGASATVWRARDTRTGRDVAL
                     KRFHAHLVSDPVARRRIEDEAAAAARVSHPSILSAIELIDDRDGLALVFPYVDGQTLA
                     ARLAQGPRITAREAASIAIDIADALAVAHDHGVVHRDVKPGNILLGADGRARLLDFGI
                     SHAVDAGQPAHELTGAGMAIGTLPYMAPEQLAAEPTTPASDVYALGVVLYEMLAGTRP
                     YEASSPVTLASEQTQPPARIADAPAPLVDLALAAMTFEVSQRPAAAQLARGLAAWRDG
                     RSDADAATSLAVATPVAARPRPALGWLAVAVAAVVLIVAVPVAASSWLAAGPDKLDLA
                     PSTIDHPVVAAAAKPTATTVPPHVVSASDTVKAGQSGGAGSGGDGGHKHHRHHDKHDG
                     KHKHGHRHRH"
     gene            6041..7309
                     /gene="rho"
                     /locus_tag="VIF08_00580"
     CDS             6041..7309
                     /gene="rho"
                     /locus_tag="VIF08_00580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8134506.1"
                     /translation="MNINELKSKNFKQLVEIGGELDVTDAKTMRPDDLVERIMARQAE
                     QGGQVFVTGILDIVDDGYGFLRRRGLMPSPDDVYVSSSQVRRSGLRAGDRVGGIIRQP
                     KDGEKYWGLVRVDSVNGVDPEVAKRRPFFDALTPIHPIELINLETDPKNLSQRLINLV
                     NPLGKGQRALIVSPPKAGKTFLLKHIANGISVNYPEMLLMVALIGERPEEVTDMRRSV
                     KGEVIASTFDEPTENHTRTAEVALEIAKRQVETGRDVVILLDSITRLARAYNLALPPS
                     GRTLSGGIDPIALYPPKRFFGAARKIEEGGSLTIIGTCLIDTGSRMDDVIYEEFKGTG
                     NSELILDRKLAEKRVFPAIDVQRSGTRREELLLEEGTLKMVWTMRRMLSAVGTNEGIE
                     LLLNRLAKSNTNAEFLASLTKAAAVEEAAR"
     gene            7497..8786
                     /gene="obgE"
                     /locus_tag="VIF08_00585"
     CDS             7497..8786
                     /gene="obgE"
                     /locus_tag="VIF08_00585"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY8134507.1"
                     /translation="MFLDEVSITVRAGDGGDGAATFRREAHVPRGGPDGGDGGRGGSV
                     YLVVDAGETMLRDYRHKHHFRADSGGRGEGSKRHGKAGRDLHLKVPPGTVVRDATTGD
                     LLADLVSREQTIMVARGGRGGLGNVHFATSINRAPTHSQKGEPGEEHTLRLELRLIAD
                     VGLVGLPNAGKSTLLAALSNANPEIANYPFTTLSPNLGVLDLSANDPSDERRITIADM
                     PGLIEGASSGAGLGHAFLRHVSRTRVLGHVVDLAAADPERDYEVIREELEAHDPTLLD
                     KVSVVIGNKIDLPAGAENQSAFAKRRKRDGIPFVAISADAGTNIDKLIATLAELLPDA
                     ATLAQPTEPAGVVVHRFDAGPNAFEVSRENGAYRVIGRRIERLAAQTNFDNAESAERF
                     QRDLARMGVERELVKAGVTSGDTVRIGGVELEWDADA"
     gene            8783..9391
                     /gene="nadD"
                     /locus_tag="VIF08_00590"
     CDS             8783..9391
                     /gene="nadD"
                     /locus_tag="VIF08_00590"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY8134508.1"
                     /translation="MTRERHGILGGLFDPIHYGHLTIAEQARDELGLTTVLFVPAGRP
                     VHRHPPDADSMHRMRMVEMAIADNPAFEASWLEVNADRPSYTVETLEVLSAASPQRDF
                     VLIVSSETAALMPTTWRGMDRILDLARIAVANRLGFTDITPDWVREHFANREDRFELL
                     STSRLGHSSTDIRARVAAGRSIRYLVPQAVAAYIEENHLYGA"
     gene            9381..9842
                     /gene="rsfS"
                     /locus_tag="VIF08_00595"
     CDS             9381..9842
                     /gene="rsfS"
                     /locus_tag="VIF08_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY8134509.1"
                     /translation="MARKTTREMAIEESPEPTPLRSGLPRRSKPVPRASATPDEEALA
                     LARRIVELAEDKKASDIVLLEIGGLTTLADYFVICSGQSERQLGAIVDGIASALRDEG
                     IKPVGREGKANAHWLLIDFGSVIVHVMAPPERDFYQLEKLWADAPLLLRLQ"
     gene            9921..10178
                     /locus_tag="VIF08_00600"
     CDS             9921..10178
                     /locus_tag="VIF08_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134510.1"
                     /translation="MARVGNACPVCSRSLVSSRFDTTFRLPDGDERYFFGIPASLCAK
                     CQQLYLDPDLIQLLDVPDARCTFAIENDQVLLTEAWSSADF"
     gene            complement(10214..11323)
                     /gene="holA"
                     /locus_tag="VIF08_00605"
     CDS             complement(10214..11323)
                     /gene="holA"
                     /locus_tag="VIF08_00605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY8134511.1"
                     /translation="MPTATAAPPLAYFWGEDAWSIERAARDYRAALEADAGQPFDVWR
                     APSDEDEGDAAATEGGSKKKTRALDEIGQHLATATLFGGGTLVIVRQPGWLLRESASR
                     ERLTKLLPEVAPGNALCFTELLAQGGKATAPSTEMRNTIEALGGQVREFAALNAARME
                     GWIDERSRELEFALAPGAARLLAERVGAYVREGDVDRRRMSELANAELEKLALYRPGG
                     TVTKDDIDALVGEVVPGSTWAFLDALGSRNIQLASSLAAKLLNEATPIPVLITQIHRR
                     LRELVVIRDHLAAGTKPPDIVRELKLNPYRAQKLSEQARTWSQEELDAALQSLYELDL
                     LTKGIALDGSPRSLSEDRSNLALLAWIGAHANPRR"
BASE COUNT         1867 a         3946 c         3825 g         1810 t
ORIGIN      
        1 ccggctggac cccgctgcga acccccgtgc cgacccccta gcgctcccct ggccgaccct
       61 cggttgcgtc agatgctggc gccgtggctg gctattgagg tacactgact tcccgagtgt
      121 ggcgttggcg tccgtgctcg cgcgttcccg gaaaaggcgc gctctggcca cgacacgagg
      181 gtctggatag atggcgaagt atgtctttgt gaccggaggc gtgacctcct cgctcggcaa
      241 aggcatcact gccgccagca tcggccggct gctcaagagc cgcggactct ctgtctcgat
      301 catcaagctc gatccatata tcaacgtgga tccgggcacg atgagcccgt accagcacgg
      361 tgaggtgttc gtcaccgacg acggtgcgga gacggacctt gacctgggtc actatgagcg
      421 cttcatcgac gagaacctga cccagctgtc gaacgtcacg acagggcgca tctaccaggc
      481 ggtgattgcc aaggagcgcc gcggcgacta cctgggcggc actgtccagg tcatcccgca
      541 catcaccaac gagatcaagg agcgcgtcgt gcgcgtcgcg cgcgacggca aggccgacgt
      601 ggtcatcgtg gaagtcggtg gcaccgtggg cgacatcgag tcgctgccct tcctcgaggc
      661 gatccgccag ctgcgcaagg acgtcggccg gacgaacgtg atgtacgtcc acgtgacgct
      721 gctgccggcg ctgtccgcga cgggcgagct gaagaccaag ccgacccagc acagcgtcaa
      781 ggagctgcgc ggcatcggca tccagcctga cgtgatcatc gcccgcgcgg accaggacgt
      841 gcctgacgac ctgcgcgaga agatcgcgct gttctgcgac gtggcggtcg aggcggtcat
      901 ccccgcgccc accgcggaga cgatctacga ggtgccgctg caattccatg agcgcggcct
      961 tgatgacctg atcgtgcgcg agcttggcct ggaggagaag gccaagccgg ccgacctgac
     1021 ggcctggcgc gacctcgtcg agctgatcaa gaagcccaag ccgcagctcg agatcgcgct
     1081 ggtcggcaag tacatcgagc tgcccgacgc gtacctgtcc gtcagtgagg cgatgcgcca
     1141 cgccggctgg gcgcacggcc gctcgatcgt catccgatgg gtggattccg aagcgctgac
     1201 ggctgacaac tatgaatcgg tgctgtccgg tgccgcaggg gtgctcgtgc ccggcggctt
     1261 tggccatcgc ggcatcgagg gcaaggtcct ggccgcgcga tacgcgcgcg agcacaaggt
     1321 gccctacctg ggtttgtgcc tgggcctgca gtgcggcgtg atcgagttcg cgcgcgacgt
     1381 cgtcggcgcc aaggacgcca actcgacaga gttcgacctc ttcaccaacg ctccggtcat
     1441 cgatttcatg cccgaccagc gcgacatgga ggagaaaggc ggcacgatgc gcctggggct
     1501 ctacccggcc aagctgacgc cgggctccaa ggccgcggcc gcatacggcg aagaggtcat
     1561 ctacgagcgc caccgccatc gcttcgaggt atcgaatcag taccgtgagg cgctcgagaa
     1621 gggcggcatg atcctgtccg gccagtcccc cgacggacgg ctcgtcgaga tcgtcgagct
     1681 cagggaccac ccctggttcg tggcatcgca attccacccc gagttccgct cgcgccccga
     1741 ccggccgcac ccgctcttcg acggcttcgt ccaggcagcg atcgatcagg ccacgcggac
     1801 cggaatcccg atcaccacgg acgcagtccc agcgaccgcc tgagagaaaa cccgaccaac
     1861 atgaccgctg ttctcgatcc attcgtcctc gagcgcgcac ccggcccggt ggtcgggctg
     1921 catccggcgc tcgtccgttt gcgcgacgct ttcaacggca cgctcgatgt gctcggcagg
     1981 atcccagatg agcggctcac ctcactgtgg gcctgggacg gcaacgacat caacgtccgc
     2041 tacgccttct atcggatgct ggagacgctc gaatcggggg ctgcgcaggt cgcactcgcg
     2101 atgcgtggcc aggagtcgag cgaagcccga gaggcggtcg gagcgacctc ggctgcgcgc
     2161 tgggacctcc acggcgtgct ggccgcgctg acagacgctg atcttgacgc cgatcccggc
     2221 ggcggcgagt ggacgatccg ccagacgatg gcccacatca tcggcgggca gcgcggctat
     2281 gcctggggca gcgcgtactg gatctccgtc cgcgacgagc ccaagcccga aggtccgcgc
     2341 cgcgcgcccg accacctctt cgcgtccatg cccgaggacg aggaggaggc gaccggcaca
     2401 ctggccgagg tgcgccagaa gctcgacgac atcgtcgatg cggcctcgtc gcgtttcgcc
     2461 accctcagta ccgacgagat ggactcggcc ggcggctggt acggcttccc ggtcaccgtc
     2521 agtttcagga tgtggcgctg gccgtcgcac atcgaggagc acacggtcca ggtcgagaag
     2581 acgctcgaca tgctcagcca ccgcgcgact gaggttgagc ggctcgtccg cctcaacgcc
     2641 cgcgcgctgg gccggctcga ggcgctggtc tttggtcgtg cgcctgagca gctggatgcg
     2701 gctggtgttg gcgcgactct tgatcgcttg gcgggcgaga tacaacaaat ggccgaaccg
     2761 gtgaaggccg cggctgatgc aggggtgccc gggccgccgg attgagcgga ggcggtcagt
     2821 cgatcgcgcg gaacagccgg tagaacgtcg gccggaaacc cgccgcgcgc caggttcgat
     2881 ctgcctccag gttcgtcgag cgccagtcaa cagcgaccca ctcgtggccg ttgtcgcgcg
     2941 cccagacgag acatgcgtcg ccgagcgcgc ggcctgcgcc caggccgcgc gcgtcccgcg
     3001 ccacggcggc ataggccagg aacccggcgc tgaccggccg catcagcggc gtgttgccgt
     3061 ccgacatgtc cagcgagcag aacgtggccg tccccacgac gcgcccctgc gcctccgcca
     3121 cgtacggcac aaggcgtggg tcttcgatct cttcttcgac atccgcacgg gccgcttccg
     3181 gggtgctgcg cagtacgctc gaaaacaccg gcgactgccc ggagtgcgtt ggcaggattg
     3241 gttcgagcgc cacgatcgcg tcgatgtcgc gcttctccgc cggccgcacc acgagcgcgc
     3301 cggcgcgcac aacgaagtcg gcgggcggaa gctcgcgcag cgcgtggacg tgctgcagcc
     3361 cgaagctcag gctgaaccac gcatcgaccg ttctcgcgtc agtcgccggc gtgaccgcgt
     3421 agtgatgggt cagccctgcg gccgcccagc gcgcggcggc gtcggcgtac gcgactcgga
     3481 tcgcctcagg gtcactcccc gcgctgccgg cgtcctcgac ccacacgttc gcgccccact
     3541 tcgcgtccct gggcgcgccc accacatagg cgaccggctc gccatctgcc agcgttatcg
     3601 agcctgacgc gccctcgcgc tcaaggagtg cggcgaccaa tggccgcgtc gcgtccgggg
     3661 cctcatatgc tggattgagt gccggccagg ccagcctgtg ccgacggtgt ctgtccgcga
     3721 ggagctcggc tgcagcgtcc aggtcggccg gtccgaagga gcgaacttcg tagcgcgtca
     3781 ccggcggact ttacggcgcc agccagcgac ccgcgtacca tgcccgaacg aaagaacaga
     3841 ggtgaaggca tgaagatctt cctggacacc gccgacatcg aagagatccg cacggccgcg
     3901 cgctggggcg tgctcgatgg cgtgaccacc aacccgtcgt tgttcgccaa gacgagtggc
     3961 aagacctacg acgaggtgct cgtcgagatc tgcggcatca cctccgggcc ggtcagcgca
     4021 gaggtcgtgg ccgaggacat cgagggcatg ctcaaggaag gtcggcactt cgcgaagctg
     4081 gcgccgaaca tcgtcgtcaa ggtgccgatg agcgaggagg gcctggaggc gatctaccga
     4141 tttgccgaag agggcatcaa gaccaactgc acactgatct tcaccgccaa ccagggcctc
     4201 ctggctgcca aggccggggc gtcattgctc tcgccctttg tcggccggct cgatgacatc
     4261 aaccaggacg ggatggacgt catccgcgag ctcgtgacga tcgtggaggt gcacgatctc
     4321 gatacggagg tcctcgcggc gtcgatccgt cacccgcgcc acatgaccga cgcggcgctg
     4381 ctcggtgccc acatcgccac gacgcccttc aaggtcctgc agcagatggt tcaccacccg
     4441 ctgaccgaca agggcatcaa gaccttccgc gaggactggg agaaggcacg caaggccgcc
     4501 gaggcgaacg ccaagtcaga ggcaagccgc cacaactagc tcgggccggc ggtcagtgcc
     4561 ggtgacgatg tccgtgcttg tgtttgccgt cgtgcttgtc gtggtgccgg tgatgtttgt
     4621 ggccgccgtc accgcctgag ccagcaccgc ctgattggcc cgctttcaca gtgtcgctcg
     4681 cgctcacgac atgtggcggc acggtcgtag ccgtcggctt ggcggccgcc gcaaccaccg
     4741 ggtggtcgat cgtcgagggc gccaggtcga gcttatcggg cccggcggcc agccagctgg
     4801 atgccgcgac gggaaccgcg actatcagaa cgaccgcagc gacggcaacc gcgagccagc
     4861 ccagcgcggg ccgcggtcgc gcggccaccg gcgtcgcgac ggcgaggctc gttgccgcgt
     4921 cggcatcgct tcgtccatcg cgccatgcgg cgaggccgcg ggcgagctgt gcggccgctg
     4981 ggcgctggct gacttcaaac gtcatcgccg ccagcgccag gtccacgagc ggggccgggg
     5041 cgtccgcgat tcgcgccggc ggttgcgtct gctcgctggc cagcgtcacg gggcttgacg
     5101 cctcgtaggg ccgcgtgccg gcgagcatct cgtacagcac cacgcccagc gcgtagacat
     5161 cgctcgcggg agtcgtcggt tccgcggcga gctgctccgg cgccatgtag ggcagcgtcc
     5221 cgatggccat gccggcgccc gtcagctcgt gcgccggctg gcccgcgtcg acggcatgcg
     5281 agatgccaaa gtcgagcagg cgcgcccgcc cgtccgcgcc aaggaggatg ttgccgggtt
     5341 tgacgtcacg gtggacgacg ccgtggtcat gggccaccgc cagcgcgtcg gcgatgtcga
     5401 tcgcgatgga cgctgcctcg cgcgccgtga tgcgcgggcc ctgggcaagc cgcgcggcga
     5461 gagtctggcc gtcgacgtat ggaaagacga gcgccaggcc gtcgcgatcg tcgatgagct
     5521 cgatcgctga caggatgctc ggatggctga cccgcgctgc agcggcagcc tcgtcttcga
     5581 ttcgcctgcg cgcgactgga tcagacacga ggtgcgcgtg gaagcgcttc agggcaacgt
     5641 ctcgaccggt cctggtatcg cgcgcccgcc agacggtcgc gctcgcgccg tggcccagtt
     5701 cttcgcgcgg ctggtagcgg ccccgcgtgg tgcgcagggt gcgggtgggt gcgtcggcca
     5761 ttcatgcatg gtcagccggc aggggtcgcg gccgccaaaa cccggcgcgc tgaggcgggt
     5821 gtcagtcggc tttcaaggtc gtcaaacgcc acggataggg ctttcgcacg ccgcttgacg
     5881 tggtatagtg cttgcacccc cgaaacagag cgactcggca gcgccgcgtc cattggggag
     5941 agattcctac aggccgcgcc tcacgtatgg cgtgcgccgc ggccccaccc gtgcccggtc
     6001 tctttcgtcc gcgcataccc gtatccacaa caggatttcg atgaacatca acgaactcaa
     6061 gagcaagaac ttcaagcagc tggtcgagat cggcggcgaa ctcgatgtca ccgatgcaaa
     6121 gaccatgcgt cccgacgacc tcgtcgagcg catcatggct cgccaggcgg agcagggcgg
     6181 ccaggtcttc gtgaccggca tcctcgacat cgtcgacgac ggctacggct tcctgcgtcg
     6241 gcgcgggctc atgccctcgc ccgatgacgt ctacgtctcg tcctcgcagg tgcgccgatc
     6301 tggtttgcgc gcgggcgacc gcgtcggcgg catcattcgc cagcccaagg acggcgagaa
     6361 atactggggc ctggtccgcg ttgactcggt caacggcgtc gaccccgagg tagccaagcg
     6421 ccggccgttc ttcgacgccc tgacgcccat ccatccaatc gagctgatca acctcgagac
     6481 agaccccaag aacctttcgc agcgactgat caacctggtc aatccgctcg gcaagggcca
     6541 gcgcgcgctg atcgtgtcgc cgcccaaggc gggcaagaca ttcctgctca agcacatcgc
     6601 caacggcatc agcgtcaact accccgagat gctgctgatg gttgcgttga tcggcgagcg
     6661 gcccgaggaa gtgaccgaca tgcggcgcag cgtcaagggt gaggtcatcg cctccacctt
     6721 cgacgagccg acggagaacc acacgcgcac cgcagaggtc gcgctggaga tcgcgaagcg
     6781 ccaggttgag accggccgcg acgtggtcat cctgctcgac tcgatcaccc gcctggcgcg
     6841 cgcctacaac ctggcgctgc cgccatccgg ccgcactttg tccggcggca tcgatcccat
     6901 cgcgctgtat cccccgaagc gcttcttcgg cgccgcgcgc aagatcgagg agggcggctc
     6961 gctcacgatc atcggcacgt gcctcatcga caccggctcg cgcatggacg acgtcatcta
     7021 cgaggagttc aagggcacgg gtaactccga gctcatcctc gatcgcaagc tggccgagaa
     7081 gcgcgtcttc ccggcgatcg acgtccagcg ctcgggcacc cggcgcgagg agctgctgct
     7141 cgaagagggc actctcaaga tggtctggac aatgcgccgc atgctttccg ccgttggcac
     7201 caacgagggc atcgagctgc tgctcaatcg cctcgccaag tccaacacga acgccgagtt
     7261 cctggcctcg ctgaccaagg cggccgcggt cgaagaggcg gcgcgctaag gcgcgctgcc
     7321 ccgctgagcg ggtcaaccgt ccgccgaacc tcgcgccgac gcggttcttg ggccgtcggg
     7381 ccgtgcagcg ccccgcttac cggggcaaat gatggctggc gactcagccg ggggaccgtt
     7441 caaccgctga gcggggcact cccgccacct gtccgcccaa actaggattc aaaacgatgt
     7501 ttctggacga ggtctcgatc accgttcgcg ccggtgacgg tggcgacggc gccgcgacct
     7561 ttcggcgcga ggcacacgtt ccgcgcggcg gacccgatgg cggcgatggc ggccgcggcg
     7621 gttcggtgta cctggtggtg gacgcgggtg agaccatgct gcgcgactac cggcacaagc
     7681 accacttccg cgccgactcc gggggacgcg gcgagggttc caagcgccac ggcaaggccg
     7741 gtcgcgacct gcatctcaag gtgccgccgg gaacggtcgt cagagacgcg accacgggtg
     7801 acctgctggc cgacctcgtg tcgcgtgagc agacgatcat ggtcgcccgc ggcggccgcg
     7861 gcggactggg caacgtccat ttcgcgacgt ccatcaaccg cgcgccgacg cattcccaga
     7921 agggcgagcc gggcgaggag cacacgctgc gcctggagct ccgactgatc gccgacgtcg
     7981 gattggtcgg cctgcccaat gccggcaagt cgacgctgct cgccgcgctg tccaacgcca
     8041 acccggagat cgcgaactat cccttcacga cgctcagtcc caacctgggc gtgctcgacc
     8101 tgagcgcaaa cgacccatcc gacgagcggc gcatcacgat cgccgacatg cccgggctga
     8161 tcgaaggcgc cagcagcggg gcgggtcttg gccatgcctt cctgcgtcac gtgtcacgga
     8221 cgcgagtcct gggccatgtc gtcgatctcg cggcggctga tccggagcgc gactacgagg
     8281 tcatccgcga ggagctcgag gcgcacgacc ccactttgct cgacaaggtc agcgtggtga
     8341 tcggtaacaa gatcgacctg ccagcgggcg cggagaacca gtccgcgttc gcgaaacgcc
     8401 gaaagcgcga cggcattcct tttgtggcca tctcggcgga cgcggggacc aacatcgaca
     8461 agctgattgc caccctcgcg gagctgctgc ctgacgccgc gacccttgcc cagcccacag
     8521 agccggccgg cgtcgtcgtc catcgtttcg acgccggtcc caacgctttc gaggtcagcc
     8581 gcgagaacgg cgcctaccgg gtgatcggcc ggcgcatcga acggctggct gcgcagacca
     8641 attttgacaa cgctgaatcc gctgagcggt tccagcgtga cctcgctcgg atgggcgtcg
     8701 agcgtgagct ggtcaaggcc ggcgtgacga gcggcgacac ggtgcggatc ggcggcgtcg
     8761 agctcgaatg ggacgccgac gcttgacgcg cgagcgccac ggcatcctgg gtgggctatt
     8821 cgatccgatt cactacggcc atctcacgat tgccgagcaa gcgcgcgacg agctgggcct
     8881 cacgaccgtc ctcttcgtcc cggccggccg accagttcac cgccatccgc ccgatgccga
     8941 ctccatgcac cgtatgcgca tggtcgagat ggcgatcgcc gacaaccccg cattcgaagc
     9001 gagttggctc gaggtcaatg ctgatcggcc gagctacacc gtcgagacgc tggaggtgtt
     9061 gtcagcggcc agtccgcaac gcgatttcgt gttgatcgtc tcgtccgaga cggcggcgct
     9121 gatgccaact acctggcggg gcatggaccg catcctcgat ctcgcccgga tcgccgtggc
     9181 gaatcggctc ggctttacgg acatcacgcc cgactgggtg cgcgagcact tcgccaaccg
     9241 ggaggaccgc ttcgagttgc tcagcacaag ccggctcggc cattcgtcga ctgacatccg
     9301 cgcccgggtt gcggccggca ggtccatccg ctatctagtg ccgcaagccg tcgcggcgta
     9361 catcgaagag aatcacctct atggcgcgta agaccaccag ggaaatggcc atagaggaat
     9421 cgcctgagcc gacgccgcta cgcagcggcc tgccgcgccg cagcaagccc gtgccgcggg
     9481 ccagtgccac cccggacgag gaggcactcg ccctggcgcg gcgcatcgtc gagctcgcgg
     9541 aggacaagaa ggcgtcggac atcgtgctgc tggagatcgg tggcctgacc accctggccg
     9601 actacttcgt catctgctcg ggtcaatcgg aacgccagct gggcgcaatc gtcgatggca
     9661 tcgcgtcggc cctgcgcgac gaggggatca agcctgttgg tcgggaaggc aaggcgaacg
     9721 cgcactggct gctgatcgac ttcggcagcg tcatcgtcca tgtcatggcg ccgccggagc
     9781 gcgacttcta ccagctcgag aagctctggg ccgacgcgcc gctgctgctg cgcctgcagt
     9841 aactgcgtac cccatccggc ggtgggcata ggtaccgacg ggtcatgtgg agtgcaccgc
     9901 accatacata cagtcggtgc atggcacgcg tcggaaatgc ttgtccggtc tgctcgcgca
     9961 gcctggtcag ctcgcgcttc gataccacct tccgtctgcc cgacggtgac gagcgctact
    10021 tctttggcat tccggccagc ctgtgcgcga agtgccagca gctgtacctc gatccagacc
    10081 tgatccagct gctcgatgtg cccgatgctc ggtgcacctt cgcgatcgaa aacgaccagg
    10141 tgctcctgac cgaggcctgg tcgagcgccg acttctagct cccttcctgg cttcgcctct
    10201 ctcccacgcc aacctacctc ctcgggttgg cgtgcgcccc gatccaggcg agcagcgcca
    10261 ggttcgagcg gtcctcgctc aacgatcgcg gcgacccgtc cagggcgatg cccttcgtga
    10321 gcaggtccag ctcgtacagc gactgcagcg ccgcgtccag ctcttcctgg ctccaggtgc
    10381 gcgcctgctc ggacagcttc tgcgccctgt aggggttgag cttcagctcg cgcacgatgt
    10441 cgggcggctt ggtaccggcc gcgagatggt cgcggatcac cacgagctcg cgcaggcggc
    10501 ggtggatctg cgtgatcaac acgggtatgg gcgtcgcctc gttcagcagc tttgcggcga
    10561 ggcttgacgc gagctggatg ttgcggctgc cgagcgcgtc caggaaggcc cacgtcgagc
    10621 ccggcacgac ctcgccgacg agcgcgtcga tgtcgtcttt ggtgaccgtg ccgccggggc
    10681 ggtatagggc cagcttctcg agctccgcgt tggcgagctc gctcatgcgc cgccggtcaa
    10741 cgtcgccctc gcgcacgtag gcgccgacac gttcggcgag caggcgcgcg gcgcccggcg
    10801 ccagcgcgaa ctcgagctcg cgtgagcgct cgtcgatcca accttccatg cgtgcggcgt
    10861 tgagcgcagc gaattcgcgc acctgcccgc ccagcgcctc gatcgtgttg cgcatctcag
    10921 tggacggcgc ggttgccttg ccaccctgcg cgaggagctc ggtgaagcac agcgcgttgc
    10981 ccggcgcgac ctcgggcagc aacttggtca gccgttcacg acttgcgctc tctctgagga
    11041 gccagcccgg ctggcggacg atgaccagcg tcccaccgcc gaacagcgtt gccgtggcca
    11101 ggtgctggcc gatttcgtcg agagcgcgtg tcttcttctt gctgccaccc tcggtcgcgg
    11161 cggcatcgcc ctcgtcctcg tcgctgggcg cccgccacac gtcgaacggc tggcccgcgt
    11221 cggcctccag cgccgcgcgg tagtcgcgcg ccgcgcgctc gatcgaccac gcgtcctcgc
    11281 cccagaagta ggcgagcggc ggcgcggcgg tcgcggtggg catctaggcc gcgcgcgcct
    11341 ggcgcagagc ggcatcaacc caggggaggc gctctacttc gtcgggcgtc acgccggcca
    11401 gcgcgcacag ctcggcctcc acccggcgaa agcgctcatg cgcgatcg
//
LOCUS       DATBRX010000017         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3134521, whole genome shotgun sequence.
ACCESSION   DATBRX010000017 DATBRX010000000
VERSION     DATBRX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3134521"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /gene="mdh"
                     /locus_tag="VIF08_00610"
     CDS             <1..1006
                     /gene="mdh"
                     /locus_tag="VIF08_00610"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY8134512.1"
                     /translation="YNRGNPDHGPSSVAQHEEMPVMARPKVTVVGAGNVGASVAQRVA
                     EGALADVVLIDIVEGLPQGKALDLAEAAPVVGHDMTITGTNDYADTAGSDIVVVTSGL
                     ARQPGMSRDDLLAKNAGIVRSVVENAVKHSPDAILIIVTNPLDAMCHVALDASGFPAE
                     RVIGMAGVLDSARFRTFIARELNVSVSSTHAFVLGGHGDTMVPLPRYSTAGGVPITEL
                     MSAERIAALVDRTRTGGAEIVALLKTGSAYYAPAASVAQMVDSILNDRNEILPCAVLL
                     KGKYGVDGLFVGVPVKLGRGGVSEIVEIELTADEQAAFDKSAGAVRELVDAMARLAAG
                     "
     gene            1098..1784
                     /locus_tag="VIF08_00615"
     CDS             1098..1784
                     /locus_tag="VIF08_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HEY8134513.1"
                     /translation="MPVITISRQFGAAGAPIGRALAERFGAEFLDRAIVAQAAIRAGI
                     PEHELESYDERLPTMWQRVASALAAAPEPAIAGVPFSASLAPMSVHERLVEITRGVIE
                     EAADRGNAVILGRGGAFILARHPAVFHVQLHASLDARVRYLLARVEEIPKEARPEETA
                     LRELCQTIDAARAEYISQVFGADWRDVTHYDLALDTGNLGVEKSTDLVEAAVRAAERP
                     AERPAPRAAS"
     gene            1781..3052
                     /locus_tag="VIF08_00620"
     CDS             1781..3052
                     /locus_tag="VIF08_00620"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8134514.1"
                     /translation="MRLPQGLVAFRHRNFRLFWTGMLVSLIGTWMQQVGQAWLVLKLT
                     NDPLALGLIAMAQFGPILVLGPLGGIVADSVSKRSALMVTQVAAGILAILLAGLYVTG
                     HIEVWHIFVLAVGLGIVNSFDMPIRQAFVIEMVGREDIANAVALNSTVFNGSRIVGPA
                     IAGLTIASVGIAPLFFINAASYLAVVGSLLLMRTSEMHVPTERAVVTRHVRSVVDLLV
                     EGVRYTRGNPRILLVILLVGAVSTFAMNFSVLIPLVARDVLHGDADTYGFLLSAAGVG
                     SLVSALTIAFRRTTSTRRLFVGAALVGLGMVGLGLSRWLPLSLAMMFVAGWGGIAMAA
                     TANTMIQLSVPDVLRGRVVSLYTTVFVGSTPIGGLFSGVVASGFGGAPAALIAGGSLA
                     VLAAIVAYLRVPDRPSLRATLPALPGREARN"
     gene            3067..>3435
                     /locus_tag="VIF08_00625"
     CDS             3067..>3435
                     /locus_tag="VIF08_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134515.1"
                     /translation="MLAVIELNFDPLFRIAGMTIRWQTIGVTIALLLALAVAALMAPD
                     AGSQRPFFRRREPDLRPLFPQAPTIERSRRVRPLRLDDMLLILAGIVPGAVVVGRALH
                     GLDFWSYYASQPGKLFDPSVG"
BASE COUNT          464 a         1213 c         1174 g          584 t
ORIGIN      
        1 gtacaatcgg ggcaatccag accacggccc cagcagcgtg gcccagcacg aggagatgcc
       61 tgtaatggcg cgtcccaagg tcaccgtcgt cggcgccggc aacgtcggcg cgtcagtcgc
      121 ccaacgcgtc gccgagggcg ccctcgcaga tgtcgtgctg atcgacatcg tcgagggcct
      181 gccccagggc aaggcgctcg acctggcgga ggcggcaccg gtcgtcggcc acgacatgac
      241 gatcaccggc acgaacgact acgccgacac ggcgggctct gacatcgtcg tcgtcacgtc
      301 cggcctggcg cggcagccgg gcatgagccg cgacgacctg ctcgccaaga acgcgggcat
      361 cgttcgctcg gtggtcgaga acgccgtcaa gcactcgccc gacgcgatcc tgatcatcgt
      421 caccaacccg cttgacgcga tgtgccacgt cgccctcgac gcgagcggct tccctgcgga
      481 gcgcgtcatc ggcatggcgg gcgtcctgga ctccgcgcgc ttccggacct tcatcgcgcg
      541 cgagctcaac gtgagcgtca gctcgaccca cgccttcgtg ctgggtggcc acggcgacac
      601 gatggtgccg ctgccgcgct actcaaccgc cggcggcgtg ccgatcaccg agctcatgag
      661 cgccgagcgc attgcggcgc tggtcgaccg cacgcggacg ggcggcgcgg agatcgtcgc
      721 gcttctgaag acgggcagcg cctactacgc gccggcagcg tccgtggcgc agatggtcga
      781 ctccatcctc aacgaccgca acgagatcct gccctgcgcg gtgctcctca agggcaagta
      841 cggggtcgac ggcctgttcg tcggtgtccc cgtcaagctt ggccggggcg gtgtcagcga
      901 gatcgtggag atcgagctga cggccgatga gcaggctgcc tttgacaagt cggccggtgc
      961 ggtgcgcgag ctagtggacg cgatggcgag gctggccgcg gggtaatgcc tttcctccgc
     1021 agctggcctc cggctgctgc tgcgcgcgaa ccggttggct cccgacctgt ggtcggtgtc
     1081 caaccgtcgc ttgctccatg cctgtaataa cgatctcgcg gcagttcgga gcggcgggcg
     1141 cgcccatcgg ccgggcgctg gctgagcgct ttggcgccga gttcctggat cgggcgatcg
     1201 tcgcccaggc agccattcgt gccggcatcc ctgagcacga gctggagtcc tacgacgagc
     1261 ggctgccaac gatgtggcag cgcgtagcgt cggccctggc cgccgcgccg gagccggcca
     1321 tcgccggcgt gccgttctcg gcctcgctcg ccccgatgag cgtccacgag cggctcgtcg
     1381 agatcacgcg cggggtcatc gaggaggcgg ccgatcgcgg caacgccgtc atcctgggtc
     1441 gcggcggcgc gttcatcctc gcccggcatc ccgccgtgtt ccacgttcag ctccacgcgt
     1501 cgctcgacgc gcgcgtgcgc tacctgttgg cgcgggtgga ggagatccca aaggaggcgc
     1561 ggcccgagga gaccgcgctg cgcgagctct gccagacgat cgacgcggcc cgggccgagt
     1621 acatcagcca ggtctttggc gcggattggc gcgacgtgac gcactacgac ctggcgctcg
     1681 acacgggcaa ccttggggtc gagaaatcga ccgacctggt cgaggccgcc gtccgcgcgg
     1741 ccgagcgccc cgccgagcgc cccgctccgc gcgccgcctc atgaggctgc cccagggact
     1801 cgtcgccttc cgccaccgca acttcaggct cttctggacg ggcatgctcg tgtcgctcat
     1861 tggcacgtgg atgcagcagg tcggccaggc gtggctggtg ctgaagctga ccaacgatcc
     1921 gctcgcgctg ggcctcatcg ccatggccca gttcgggcca atccttgtgc tgggaccact
     1981 gggcgggatc gtcgccgact cggtctcgaa gcgcagcgcg ctgatggtca cgcaagtcgc
     2041 ggccggcatc ctggccatcc tccttgccgg gctgtatgtg accggccaca tcgaggtctg
     2101 gcacatcttc gtcctggcag tcggcctggg catcgtcaat tcattcgaca tgcccatccg
     2161 ccaggcgttc gtgatcgaga tggtcggccg cgaggacatc gccaacgcgg tggcgctcaa
     2221 cagcaccgtc ttcaatgggt cgcgcatcgt cggcccggcg atcgccggcc tgaccattgc
     2281 gagcgtcggc atcgcgccgc tcttcttcat aaacgcggcg agctacctgg cggtggtggg
     2341 gagcctgttg ctcatgcgca cgagcgagat gcacgtaccg accgaacgcg cggtcgtcac
     2401 ccgccacgtg cgatctgtcg tcgacctcct ggtggagggc gttcgctaca cccgcggcaa
     2461 tccgcgcatc ctgctcgtca tcctgctcgt cggcgcggtc tcgacgttcg ccatgaactt
     2521 ctccgtcctg atcccgctcg tcgcgcgcga tgtgctccac ggtgacgcgg acacctacgg
     2581 cttcctgctg tcggccgcgg gtgtcggctc gctggtcagc gcgctgacga tcgcgttccg
     2641 gcgcacaacg tcgacgcgcc ggctcttcgt tggcgcggcg ctcgtcgggc tgggcatggt
     2701 tggcctgggg ctgtcgcgct ggctgccgct cagcctggcg atgatgttcg tcgcgggttg
     2761 gggcgggatc gcgatggccg cgacagcgaa cacgatgatc cagctgtcgg tccccgacgt
     2821 gctgcgcggc cgggtcgtga gcctctatac gacggtcttc gtcggctcga cgcccatcgg
     2881 cggcctgttc agcggcgtgg tggcgagcgg cttcggtggc gcgccggcag cgctgatcgc
     2941 gggtggctcg ctcgccgtgc tggccgcgat cgtcgcctac ctgcgcgtcc cggatcggcc
     3001 gtcgctgcgt gcaaccctgc ccgcactacc aggtcgcgaa gcccgaaact aggcgccgtg
     3061 ccgggagtgc ttgcggtaat cgagctcaac ttcgatccgc tgttccggat tgccgggatg
     3121 acgatccgct ggcagacgat cggcgtgacg atcgcccttc tattggccct ggccgtcgcc
     3181 gcgctgatgg cgcccgacgc gggcagccag cgacccttct tccggcgtcg tgaacctgac
     3241 ctgcggccac tcttcccgca ggcgcccact atcgagcgct cgcgccgcgt ccgaccgttg
     3301 cgcctcgacg acatgctcct gatcctcgcc ggcatcgtgc ccggcgcggt cgtggtgggc
     3361 cgcgccctcc acgggctcga cttctggtcg tactacgcga gccagcccgg gaagctgttc
     3421 gatcccagcg tcggc
//
LOCUS       DATBRX010000018         4094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1570039, whole genome shotgun sequence.
ACCESSION   DATBRX010000018 DATBRX010000000
VERSION     DATBRX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570039"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VIF08_00630"
     CDS             <1..1107
                     /locus_tag="VIF08_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY8134516.1"
                     /translation="SLIVISGPPLATPASVLAGAVHDAVAHLPRRLSVATVFMSTEQP
                     RAALSGEGLTIPSYPYPENAAYALGHAVRYGAWRAAPAGRVREFADICAADLPPRVDG
                     ESRWLAPNVVRRLLTCYGLPVVEEGFVPSGAETREIAATMSGPYALKGVSTAVVHKSE
                     ARGVRLDLATPADVEQAAREMTADYAAAGHAIEGFMVQPMVPSGIELLVGVVHDRLFG
                     PVVACAIGGTQTELLKDVSVRITPLTDVDAAQMVRSLATYPLLDGYRGAPPLDVAALE
                     EVLLRVSALVEAHPEIAEMDLNPVIVLPRGAVIVDARIRIEPAGSIERGILRDQENTA
                     SSGTRQSERDHREPGQQLEARAAPSENAPPVSDR"
     gene            1232..3085
                     /gene="thrS"
                     /locus_tag="VIF08_00635"
     CDS             1232..3085
                     /gene="thrS"
                     /locus_tag="VIF08_00635"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY8134517.1"
                     /translation="MAQEVAEHAEHEEHEEELAFEAPKRSSAEELLDAHAGDPLDPMR
                     HSAAHVMAEAVLDLFPGTKLGIGPAIADGFYYDFEVSRPLSTDDLAQIEARMAASVQA
                     DHRFERKVLQPDEGRAFFERSGQPFKVEILDDLRAAAKASGAPEPEVSTYEHGPFIDL
                     CRGPHIESTGKIGPFKLLAVSGAYWRGDQKRPAMQRIYGTVWSTQEELDAFLWRREEA
                     KKRDHRKLGVALDLFSFHDVSPGAAFWHPKGWRFYNTLRDAMRSIQAKRGYQEIYTPP
                     LVHKKLWEQSGHWDLYRENMFLVEHDDQTWSLKPMNCPESTFIYRSRVRSYREFPLRL
                     SEYGALHRAELSGVRSGLTRVRHFYTDDAHIYVTPAQIQAEILALMGEVTEFYSWFGL
                     EPLLTFGTRPAKALGDEATWRETEAMMLEALKASGVNYRVKEGDGAFYAPKIDIQIKD
                     ALGREWQTATIQVDRVMLPERFDLHYIDENGERQRPVAIHRAILGSLERFIGVITEHF
                     AGAFPFWCAPVQAIVIPIADRHNEAAAELAAVLGAKGLRVEVDDSANRMQNKIRLAQE
                     QKVPYMLVLGDREIEARTAAPRTRAGVQQEPIGWDELAARLAEEAAARKPD"
     gene            3487..>4094
                     /locus_tag="VIF08_00640"
     CDS             3487..>4094
                     /locus_tag="VIF08_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HEY8134518.1"
                     /translation="MPTPSAPPTAAVTVSPAPSAGTTVDPNLPIGGDDWGDSAPGEVG
                     ERYLYNCPPDGDARSLWGTDIYTSDSSVCTAGVHSGLITFAQGGSVVIENRPGQESYI
                     GSFRNGVTSSSYASWVRSFIFVSGSVPVPTPSTGPSGVPGTGYGADTMLFSDDFSDTS
                     GGWATESGPDGTVAYESEKLAVDLAANVSYVSTSGLIDSMTW"
BASE COUNT          688 a         1338 c         1422 g          646 t
ORIGIN      
        1 tcgctgatcg tcatctccgg cccaccgctg gccaccccgg cgagcgtgtt ggcgggcgcc
       61 gtccacgacg cggtcgcgca cctgccgcgc cgcttgagcg tcgcgacggt cttcatgtcg
      121 accgagcagc cgcgtgccgc cttgagcggg gaggggctca cgatcccgtc ctacccctac
      181 ccagagaacg cggcctatgc cctgggtcac gcggtgcgct atggcgcgtg gcgcgcggcg
      241 ccggcggggc gcgttcggga gttcgccgac atctgcgcgg ccgacctacc gccgcgcgtc
      301 gatggtgagt cgcgctggct cgcgccaaac gtggtccggc gcctgctgac ctgctacgga
      361 ctgccggtgg tggaggaggg cttcgtcccg tctggtgctg agacgcgcga gatcgcggcg
      421 acgatgagcg gtccgtacgc cctgaagggg gtctcgaccg cggtcgtgca caagagcgag
      481 gcccgtggcg ttcgactcga cctggcgacg ccggcggacg tggaacaggc ggcgcgcgag
      541 atgacggccg attacgccgc tgccggccac gcgatcgaag gcttcatggt ccagccgatg
      601 gtgccgtccg gcatcgagct cctggtcgga gtcgtgcacg accgactgtt cggcccggtg
      661 gtggcctgcg cgatcggtgg cacgcagacg gagctcctca aggacgtgtc tgtgcgcatt
      721 acgccgctga ccgacgttga cgccgcgcag atggttcgat cgctcgcgac gtatccgctg
      781 ctcgacggtt accggggagc gccaccactc gacgtcgcgg cactggagga agtgctgctc
      841 cgtgtgagtg cgctggtcga agcgcatccg gagatcgcgg agatggacct gaacccggtg
      901 atcgtcctgc cgcgcggcgc ggtcatcgtc gatgcccgca tccgaatcga gcctgcgggc
      961 tcaatcgagc gcggtatact ccgcgaccaa gagaacacag cgtcgagcgg gacgcgccaa
     1021 tcggagaggg accacagaga gccggggcag cagctggaag cccgggcggc accttccgag
     1081 aacgcaccac ccgtgagcga tcggtgaacc ggtccgggag ccgcccgtta gcgcggcagc
     1141 gcaagagcgc ctaacggcga acgcgggtgg aaccgcggga gggtcaacct ctcgtcccgg
     1201 tcgagtggat cgacggacgg gaggtttttt gatggctcag gaagtcgcag agcacgcaga
     1261 acacgaagaa cacgaggaag agctcgcgtt cgaggcgccc aagcgctcat cagctgagga
     1321 gctgctcgac gcacatgccg gcgacccact cgatcccatg cgccactctg ccgcgcatgt
     1381 catggccgag gcagtcctcg acctcttccc gggcaccaag ctgggcatcg gccccgccat
     1441 cgccgacggc ttctactacg actttgaggt cagccgtccg ctatccacgg acgacctggc
     1501 gcagatcgaa gcgcgcatgg ccgcaagcgt ccaggcggac caccggttcg agcgcaaggt
     1561 gctccagccc gacgaaggcc gggcgttctt cgagaggagt gggcagccat tcaaggtcga
     1621 gatcctcgat gacctgcggg cggcggcaaa ggcgtccggc gcgccggagc ccgaggtgtc
     1681 gacgtatgag cacgggccgt tcatcgacct gtgccgcggg ccgcacatcg agtcgacggg
     1741 caagatcggt ccgttcaagc tgctcgccgt cagtggcgcg tactggcgcg gcgatcagaa
     1801 gcgaccggca atgcagcgca tctacggcac tgtgtggtcg acgcaggaag agctcgacgc
     1861 cttcctgtgg cggcgcgagg aggcaaagaa gcgcgaccac cgcaagctgg gcgtggcgct
     1921 cgacctcttc tcgttccacg acgtctcgcc gggcgcggcg ttctggcatc ccaagggctg
     1981 gcgcttctac aacacattgc gcgatgccat gcgctcgatc caggcgaagc gcggctacca
     2041 ggagatctac acgccgccgc tggtgcacaa gaagctgtgg gagcagtcgg gccactggga
     2101 cctgtaccgc gagaacatgt ttctcgtcga gcacgacgat cagacgtggt cgctcaagcc
     2161 catgaactgc ccggagtcga cgttcatcta ccgctcgcgc gtgcgctcgt accgtgagtt
     2221 tccgctccga ctgtccgagt acggagcttt gcaccgggcg gagctgtcgg gcgtccgaag
     2281 cggcctgacg cgggtgcgcc acttctatac ggacgatgcg cacatctacg tcacgccggc
     2341 ccagattcag gccgagatcc tggcgttgat gggcgaggtg actgagttct actcgtggtt
     2401 tggcctggag ccgctgctca cgtttggaac gcggcccgcc aaggcgctcg gcgacgaggc
     2461 cacgtggcgc gagacggaag cgatgatgct cgaggcgctc aaggcgtcgg gcgtcaacta
     2521 tcgcgtcaag gaaggtgacg gcgcgttcta cgcacccaag atcgacatcc agatcaagga
     2581 cgcgctgggg cgcgaatggc agacggcgac gatccaggtc gaccgagtca tgctgcccga
     2641 acgcttcgac ctgcactaca tcgacgagaa cggcgagcgc cagcggccgg tggcaatcca
     2701 ccgcgccatc ctgggctcgc tggagcggtt catcggggtg atcacggagc acttcgccgg
     2761 ggcgttcccc ttctggtgcg cgccggtaca ggccatcgtc atcccgattg ccgatcgcca
     2821 caacgaggcc gcagccgagc tggcggccgt cctgggggcg aagggcctgc gggtggaggt
     2881 cgacgactcg gccaaccgca tgcagaacaa gatccgcttg gcgcaggagc agaaggtgcc
     2941 ctacatgctg gtcctgggcg atcgcgagat cgaggcacgc actgccgcgc cgcgcacgcg
     3001 ggcaggagtg cagcaggagc caatcggctg ggatgagctg gccgcgcggc tcgcagaaga
     3061 agcggctgcc cgcaagcccg attgattggg ccggccgcgt ctcgtgacgc cactatggtc
     3121 atgagcgcgt ggatgggctg gtgcaccggc gcccttgctt ggcgaaagcc aggatccggc
     3181 tgaggccgtt gcgctggtga gcgtggcttg cccgggaccg agcacgtctg gcggcttacc
     3241 gctgccattc ggcacggcga agggccactt ttgaccatag ctgcgtcacg gctcgatcta
     3301 agcccaatgg ggccccgtga cccaggttgg ggacttgcgc gtgcgagtag catcgccgca
     3361 ctcaggtagc catggcgggg gtaccgatcg atgctgcgat caacggtggt agcgctttcg
     3421 cttctcgtcg ttgccgcgtg cagtggagga gcaccgccca cggccgcgcc gactccctca
     3481 agcgccgtgc caacgccctc cgcaccgccg actgctgccg tcacggtctc gccggcgccc
     3541 agtgccggga cgacggtcga cccgaatctg ccaatcggcg gcgacgactg gggcgattcc
     3601 gctccgggtg aggtcggtga gcgctacctg tacaactgcc cgccggacgg cgacgccagg
     3661 tctctctggg gcacggacat ctatacaagt gactcgtcag tgtgcacggc cggagtccac
     3721 tccgggctga tcacgtttgc ccaaggcggc agcgtcgtca tcgagaatcg gcccggccag
     3781 gagtcctaca tcggcagctt ccgcaatggc gtaaccagct ccagctatgc gtcgtgggtg
     3841 cggagcttca tctttgtcag tgggtcggtg cccgtgccca cgcccagcac cggcccgagc
     3901 ggtgtcccgg gcaccggcta tggcgctgac acgatgctct tcagcgatga cttcagcgac
     3961 accagcggcg gctgggcaac ggagagcggt cccgacggca ccgttgccta cgagtcggag
     4021 aagctggcgg tcgacttggc cgcgaacgtc tcctacgtat ccacgagcgg cctgatcgac
     4081 tccatgacat ggga
//
LOCUS       DATBRX010000019         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2687229, whole genome shotgun sequence.
ACCESSION   DATBRX010000019 DATBRX010000000
VERSION     DATBRX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687229"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VIF08_00645"
     CDS             <1..713
                     /locus_tag="VIF08_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134519.1"
                     /translation="EDIFGDPSAGLDVVAGGSNLVALECAGTATGSLRLTLWLGESGP
                     VATWTQPNGPENFDRAGTYVAASGEGFSVEMDNVILFGSGVADGSYSPAGEELLAHIP
                     ADWQSSCYPGLRPPYLARTAEAVVTCFLSSSNADGAEIAEYASYLSAADMDAAYQERI
                     DAFGTGDGSSSCGDGSGEHPYDIGDTESGRLLCVDQFAGTRFDWTDTRLNILSTLIDF
                     DGDYGATLDDWLIAGPDL"
     gene            837..1415
                     /gene="infC"
                     /locus_tag="VIF08_00650"
     CDS             837..1415
                     /gene="infC"
                     /locus_tag="VIF08_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY8134520.1"
                     /translation="MNEMIRVPQVRVVDEEGNQAGVMSTPDALRLAQDRGYDLVEVAP
                     MASPPVVRLLDYGQFKYEQARREKEARKHQRSVDFKEIRLKIKIGKGDFDTKVRRSIE
                     FLEDGDRVKVTVQFRGREVSHAYLGRDLLMRFAEQVGENGTVERQPLLEGKQMSLVIT
                     SNHKPKPRAEGGETPETAAVEQPTAAAAPPPA"
     gene            1429..1632
                     /gene="rpmI"
                     /locus_tag="VIF08_00655"
     CDS             1429..1632
                     /gene="rpmI"
                     /locus_tag="VIF08_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY8134521.1"
                     /translation="MPKMKTHKGAAKRFGVTGSGKVVRVKAWRGHHLDIKSSSRTRRY
                     AGKAVVDGTSAEQIRGLLPYLGK"
     gene            1639..1989
                     /gene="rplT"
                     /locus_tag="VIF08_00660"
     CDS             1639..1989
                     /gene="rplT"
                     /locus_tag="VIF08_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY8134522.1"
                     /translation="MARVKRGVTAHRRHKRLLKAAEGRRGTKSRLIKPAREALLHAMA
                     YSYVGRKQRKRQMRALWIVRLNAAARQNGMTYAKLVAGLKRAGVALDRKILADIAVRD
                     AATFASIAEVAKAS"
     gene            2002..3045
                     /gene="pheS"
                     /locus_tag="VIF08_00665"
     CDS             2002..3045
                     /gene="pheS"
                     /locus_tag="VIF08_00665"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY8134523.1"
                     /translation="MDLASLLDGLTGLRQQALPAVEKADDPAELDAVEAAYLGRSGEL
                     RKLLANIGKLSSDDRPKVGAAANPIREELEAAIAARRARLESIALDVRLAREKTDVTL
                     PGRTLPRGSLHPLMETGREIARIWAQFGFVVYESPEVETEELNFELLNIPRDHPARDL
                     WDTMYVAGTDRHYLLRTHTSPGQMRVMHSTKPPIRALLPGRCFRYEAVDASHGFEFFQ
                     VEGLMVDEGTTLATLRGLLDEFARAMFGPNTATRFRPGYYPFTEPSVAFDIGCLICGG
                     SGCAACKRTGWLTILGAGMVHPQVLRNGGVDPEVHQGFAFGMGMDRIAMLRHGISDLR
                     VFFQGDLRFLEAF"
     gene            3046..>4745
                     /gene="pheT"
                     /locus_tag="VIF08_00670"
     CDS             3046..>4745
                     /gene="pheT"
                     /locus_tag="VIF08_00670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY8134524.1"
                     /translation="MRVPLSWLRDYVEFDLPPERLAERLTLLGLEVQSIERIGDDWTS
                     VVVGELLEVVPHPRADRLNLTKVKVTGQDQPLSIVCGAHNIAAGQRVPVALPGAVLPG
                     DRRIGVSTIQGTESQGMLCSGAELGLTTDAEGILILPPDSRLGSPLADVAGDVVLDID
                     VKPNRGDALSMIGLAREVAAITGGQVRWPEISVPESGDATSDHLGVEVTDDKLCPRFV
                     GRYVDGLKVGPSPFRIQLRLSAAGMRPVSNVVDASNYVMLELGKPIHTFDAASVTGGK
                     IVVRTARDMEELETLDHVKRSLTPDTLVIADATGAIGIAGVMGGAGSEVSETTSAVIV
                     ESAVFDPVSIRRTAFRYGLRSEASLRFEKGQESRLARVGADRTAQLLAEWAGGRVATG
                     VVDTNPVDEEPRRVAFRPARVDRLLGTDTSADEMRAALSRIGIATEGEGEELVAVVPP
                     HRRDIAIAEDVAEEVVRIIGYDAVPARLPASVMPGYREIPTEHINSLRDVLAGRGLNE
                     VLSNALIGPDDHARLGYGAGDKATIRVENHISIDHSELRRTMLPGLLGVLGRNERQKR
                     "
BASE COUNT          802 a         1585 c         1612 g          746 t
ORIGIN      
        1 ccgaggacat cttcggcgac ccctcagccg ggctcgacgt ggtggccggc ggatccaacc
       61 tggtcgcgct cgaatgtgcc ggcacggcca ccgggtcgct gcgtctcaca ctctggcttg
      121 gcgagtcggg cccggtcgcg acctggacac agcccaacgg cccggagaac ttcgatcgcg
      181 ccgggaccta cgttgctgcg tcgggtgagg gcttcagcgt cgagatggac aacgtcatcc
      241 tgttcggctc gggcgtcgcg gatggttctt acagcccggc gggcgaggag ctgctggcgc
      301 acatccccgc ggattggcaa tcgtcgtgct acccgggcct ccgaccgccg tacctggcgc
      361 gcaccgcaga agccgtggtg acctgcttcc tgtcgagctc gaacgccgat ggcgcggaga
      421 tcgccgagta cgcgtcctac ctctcagccg ccgacatgga tgcggcctac caggaacgca
      481 tcgacgcgtt cggcactggc gacggctcca gctcgtgtgg cgatggatcg ggtgagcacc
      541 catacgacat cggcgacacg gagtcgggtc gtctgctgtg cgtggaccag ttcgccggca
      601 cccgcttcga ctggaccgat acgcgcctca acatactcag cacgctgatc gatttcgacg
      661 gcgactacgg cgcgaccctc gacgactggc tgatagccgg gccggacctt tagaaaggcc
      721 tcatctgcta tattcccgcg ggccgctgaa gggtgcggcg attaacgtcg tccactctgg
      781 gagttgccat ttgagaaacc gcgcatgagg gatctggcct gagccgagag ctgagaatta
      841 atgagatgat ccgcgtcccg caggtgcggg tcgtggacga agagggcaac caagcgggcg
      901 tgatgtccac accggacgcc ctccggctcg ctcaggatcg gggctacgat ctcgtcgagg
      961 tcgcgccgat ggcttccccg ccggtcgtcc gacttctcga ttacggccag ttcaagtacg
     1021 agcaggcccg ccgcgagaaa gaggcccgga agcaccagcg gtcagtcgat ttcaaggaga
     1081 tccgcctcaa gatcaagatc ggcaagggtg acttcgacac caaggttcgg cgctcgatcg
     1141 agtttctcga ggacggcgac cgggtcaagg tgacggtcca gttcaggggc cgcgaggtga
     1201 gccacgctta ccttggccgg gacctgttga tgcgcttcgc cgaacaggtc ggcgagaacg
     1261 ggacagtgga gcgccagccg ctgctggaag gcaagcagat gtcgctcgtg atcacctcga
     1321 accacaagcc caagccacgt gctgaaggcg gagaaacacc agagacggca gcagtcgagc
     1381 agcccacagc ggccgccgcg ccgccaccag cctaggtaga tacgaaacat gccaaagatg
     1441 aagacccaca agggagccgc caagcgcttc ggcgtcaccg gcagcggcaa ggtcgtccgc
     1501 gtcaaggcgt ggcgcggcca ccaccttgac atcaagtcgt ccagccgtac ccggcgctac
     1561 gccggcaagg cggtcgtcga cggcacgagc gcagagcaga tccgcggcct cctgccgtac
     1621 ctgggcaagt aggcacagat ggcacgagtc aagcgcggcg taaccgccca ccggcgccac
     1681 aagcggctgc tcaaggcagc tgagggcagg cgcggtacca agtcacggct catcaagccc
     1741 gcgcgtgagg cgctgcttca cgcgatggcc tactcgtacg tcggccgcaa gcagcgcaag
     1801 cgccagatgc gcgccctgtg gatcgtccgc ctcaacgctg ccgcgcggca gaacggcatg
     1861 acctacgcca agctggtcgc cggtctgaag cgcgccggag tggcgctcga ccgcaagatc
     1921 ctggccgaca tcgcggtccg cgatgcggct acgtttgcca gcatcgcgga ggtcgcgaaa
     1981 gcgtcctagc ggcaccgcgc cgtggacctc gcctctctcc tggatggcct gacgggcctc
     2041 agacagcagg cgttgccggc tgtcgaaaag gcggatgacc ccgccgagct tgatgccgtg
     2101 gaagccgcct acctgggccg cagcggcgag ctgcgcaagc tcctggcgaa catcggcaag
     2161 ctgtcgagcg atgaccggcc caaggtcggc gctgcggcca accccatacg cgaggagctc
     2221 gaggcggcca tcgccgcgcg gcgggctcgc cttgagtcga tcgcccttga cgtccgtctc
     2281 gcacgcgaga aaacagacgt cacgctgccc ggccgcacgc tgccgcgcgg ctctctccat
     2341 ccgctcatgg agaccggccg cgagatcgcg cgcatctggg cccagttcgg ctttgtcgtt
     2401 tacgagtcgc ccgaggtgga aacggaggag ctgaacttcg agctgctcaa catcccgcgt
     2461 gaccacccgg cgcgcgacct gtgggacacg atgtacgtgg ccggcacgga tcgccactat
     2521 ctgctgcgta cccacacgtc acccggccag atgcgcgtca tgcactcgac caagccgccc
     2581 atccgggcgc tgctgccggg gcgctgcttc cgctacgagg cggtcgacgc cagccacggc
     2641 ttcgagttct tccaggtcga ggggctcatg gtcgacgagg gcacgaccct cgcgacattg
     2701 cgcggcctgc tcgacgagtt cgcccgcgcg atgttcggac ccaacaccgc tacccgtttc
     2761 cgtcccggct attacccatt cacggagccg tccgtcgcgt tcgatatcgg ctgcctgatc
     2821 tgcggcgggt ccggttgcgc ggcgtgcaag cgcaccggct ggctgacgat cctgggcgcg
     2881 ggcatggtcc atccccaggt gctgcgcaac ggcggcgtcg acccggaggt ccaccagggc
     2941 ttcgcgttcg ggatgggcat ggaccgcatc gcgatgctgc gtcacggcat ctccgacctg
     3001 cgcgtgttct tccagggcga tctgcgcttc ctggaggcct tctagatgcg cgtcccgctg
     3061 tcgtggctgc gtgactacgt cgaattcgac ctgccgccgg agcgacttgc tgagcgcctg
     3121 acgctgctcg gattggaggt ccagtcgatc gagcgcatcg gtgacgactg gacgagcgtc
     3181 gtggtgggcg agctgctcga ggtcgtgccc catccgcgcg ctgaccggct gaatctcacc
     3241 aaggtcaagg tcacgggcca ggaccagcca ctgtcgatcg tctgcggcgc gcacaacatc
     3301 gcagccgggc agcgcgtccc ggtggcgttg ccgggcgccg tcctgccggg cgaccggcgc
     3361 attggcgtct cgaccatcca gggcacggag agccagggca tgctctgctc cggcgctgag
     3421 ctgggcctga caacggatgc cgaggggatc ctcatccttc cgcccgactc gcggctcggc
     3481 agcccgctgg ccgatgttgc cggtgacgtc gtgctcgata tcgacgtcaa acccaaccgt
     3541 ggcgacgcgc tatcgatgat cggcctcgcg cgcgaggttg ccgccatcac gggcggccag
     3601 gttcgctggc ccgagatcag cgtgcccgag tcgggtgacg ccacgagcga ccacctgggc
     3661 gtggaggtca ccgatgacaa gctttgcccg cgcttcgtgg gtcgctatgt cgacgggctg
     3721 aaggtcggcc cttcgccatt ccgcatccag cttcgactgt cggcggcggg catgcgcccc
     3781 gtgtcgaacg tagtcgacgc cagcaactac gtgatgctcg agctgggcaa gcccatccac
     3841 acgttcgatg cggcgtcagt caccggcggc aagatcgtcg tgcgcacggc acgagacatg
     3901 gaagagctcg agacgctcga tcacgtcaag cgcagcctga ctccggacac actcgtcatc
     3961 gccgacgcga cgggcgcgat cggcattgcc ggcgtcatgg gtggtgccgg ttcggaggtg
     4021 agcgagacga cgagcgcggt gatagtcgaa tcggcagtgt tcgatccggt ctccatccgg
     4081 cgcacagcgt ttcgctacgg gctgcggagc gaggccagcc tgcgcttcga gaagggccag
     4141 gaatcgcgac ttgcgcgcgt tggcgccgac cggaccgctc agctgctggc cgagtgggcc
     4201 ggcggtcggg tcgcgactgg cgtcgtggac acgaaccccg tcgacgaaga gccgcgccga
     4261 gtcgccttca ggccggcgcg cgtggacagg ctgctcggca cggacacgag cgcggacgag
     4321 atgcgcgccg cgctgtcgcg gatcggcatc gcaacggaag gggagggcga ggagctggtt
     4381 gccgtggtgc cgccgcatcg gcgcgacatc gcgatcgcgg aggacgtggc cgaggaggtc
     4441 gtgcgcatca tcggctacga cgcggtgccc gcgcgtctgc cggcgagcgt catgcccggc
     4501 tatcgcgaga tcccgacaga gcacatcaac tcgttgcgcg acgtgctcgc cggccgcggc
     4561 ttgaacgaag tgctgtcaaa tgccctcatc ggcccggatg accacgcgcg tctcggatat
     4621 ggcgcgggcg acaaggccac gatccgcgtc gagaaccaca tctcgattga ccactcggag
     4681 ctgcggcgca cgatgttgcc cgggttgctc ggcgtgctgg gtcgcaacga gcgccagaag
     4741 cgcga
//
LOCUS       DATBRX010000020        10948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2464021, whole genome shotgun sequence.
ACCESSION   DATBRX010000020 DATBRX010000000
VERSION     DATBRX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10948
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464021"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VIF08_00675"
     CDS             <1..705
                     /locus_tag="VIF08_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134525.1"
                     /translation="GLLVELDKYVAPGSRLMVIAQGHGTDLAGEVEAVGRRLKNLELT
                     YRAADTTDRAVLDEVTSDAYGHVVVMSYSDDYDEQRADAKTLVTLLHLRDIESNRGES
                     FTIVSEMLDVRNRALAAVTRADDFIVSGKLVSLMMSQLAETPDLRPIFDDMFDEDGSE
                     IYLKPAGDYVKLGAPVDFYTVVESARRRGQIAFGYRLLGQANDPNKNYGVAMNPDKAE
                     QVTFGSDDKLIVIAED"
     gene            complement(806..2401)
                     /locus_tag="VIF08_00680"
     CDS             complement(806..2401)
                     /locus_tag="VIF08_00680"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8134526.1"
                     /translation="MSERPLPGETSSGDHRDRDHGLVESQIQAINRFPDQNPHPVLRV
                     ADDGQLTYANLSSAPVLRAMNAAVGRTMEPELLARLRAAASAGGSIELRDGVRIFAIL
                     PVPVPELGFMNLYGTDITAEKVVERFPNQNPNPVFRVDDSGTLIYANAASQPLIEAFG
                     LKVGDPWPMEIAARVLAAADAPTRDLLELEADYRTYALRPVRIPEFGFINVYGTDITA
                     EKVVAKFPGQNPNPVLRTDSSGVLIYANDASAPMRAALKFAVGEPLPGELQAQITARV
                     ATRSREPIECIAGDQFYELTAVEIPEFGFINLYGTDVTAARQVARAKEETERLLLNIL
                     PPPIANRLRSGERVIADRFEDASLLMADIVGFTELSSRLSADDLVEMLNDIFSVLDVI
                     VARYELEKVKTIGDAYMVIGGVPIADPNHLERLAGFALDMALAVRELQSGDHGAIRMR
                     IGINAGPVVAGVIGQKRTIYDVWGDTVNVASRMESLGEPGRIQVTPLVYERLKDTFEF
                     EPRGQVEVKGKGPMTTWWLTGRR"
     gene            complement(2414..2650)
                     /locus_tag="VIF08_00685"
     CDS             complement(2414..2650)
                     /locus_tag="VIF08_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134527.1"
                     /translation="MQTLIVDTLKVWREAERALEVVPPADPKHERVRKLVIELRSLYA
                     DLSATQTLPPEVISGKRARVEEAREELRHLLDGA"
     gene            complement(2768..4309)
                     /locus_tag="VIF08_00690"
     CDS             complement(2768..4309)
                     /locus_tag="VIF08_00690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY8134528.1"
                     /translation="MSDSSPERRQNKKASVAAPPPSAPTPPPPPAGATMNAGSVAAAP
                     PLPRWGVQTGFTQVRQPAFWLFVVLFAVAGLTILTEQLRYLQFFPSGWIFSVALLALY
                     VVPVGIAIWLLDLYEREPISLVLAAFVWGGVIATGLAVTINTSLIEVVAKLFGPSFAQ
                     TWGVAIVPPPVEETLKYLGVVTILLIARNEIDDVLDGFVYGAVVGLGFAAVENVQYFI
                     QAVANSGGADQIGPVLGMFFLRSLLIGAYMHVMWTGLTGAGLAYFVTRLDQPYQRRLM
                     VAVGLFLAGVVAHMLWNSPLLNGLLTDIGGIVVFGLIKGLPFLGFLIFLVILAGRREQ
                     RWFATLTQADVGSDVLTQPELNELGGLRSRWAARRRVRAIKGPQGGKLAAQLQREQIN
                     LAMIRSRTGSDDDPSMVAQRERIRTIRAQLNALPDITAAQPVAAAVAAPAIAAPGAWT
                     PTHVAPPTGLAAWETPDPSRPPMVTLAGGVQLSVLEERGAWARVMGSNGWTGWVDGRL
                     LARLG"
     gene            complement(4314..5123)
                     /locus_tag="VIF08_00695"
     CDS             complement(4314..5123)
                     /locus_tag="VIF08_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134529.1"
                     /translation="MSSEPPPPPQSPVAPPPPDPAVQPALAAPPPPPPPPGIDAGAKA
                     RALFSQLDPAGIRPTAIVAGIIIALYFGAQIVNAVIPQGGAGPGIPGPGGQGPSGPGP
                     VPTTVPGPTNGPVGPGPTSAPAQPGSTLTLGPLRIPLENGWTPQEVPDSNVIVRLVKG
                     NVAVDLYGASIQGQADAGAVYNAYLTQSLQPAATGFNASQPNLLQIGNGIPAARGTYT
                     GVFGQSQIEGQVTTIVVGANGFIFDAWGSAGTLGQLLPEAQRMIDNLQVVQ"
     gene            complement(5183..6520)
                     /locus_tag="VIF08_00700"
     CDS             complement(5183..6520)
                     /locus_tag="VIF08_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134530.1"
                     /translation="MNPRADWTLYGAVGALALVAVSILLLPILLFQPGAATGPADPNA
                     PVVGPTPPFGPGQPGVPQDPGSLDSPPQFVATGARLTWYAAAASVANSRFSWVEEPCT
                     GNNWTDPKTGKCYRRTDESGEGQGTASGDGLSQLDIVALDGSRAAISNTLYTFFRENN
                     TLIWTPVGGASVSAIDVDGAWIHPARLAQIAQSGLGDLLVLRGRYSLGTQTFDALSAV
                     SGLGTSTYSSYTYDLATGILLSANTSTGGITSPLSAPGQGAPTSNSQLTMARFAGFRT
                     RNLPGMTAPKPDWVNGTSQLIYQGTYNFTNPVDPSSANLTYPTQVTVQLGQGGATWSS
                     FTSRTETPALNINTQSQGVAGPNGPYWYDPASLAGMSAGQVLDQDGLTGERTTVSAVG
                     QDQAGTAVVSIDVQLPGNFARFTYEQSTGMLVEFTIQAANAGTTTTARLVERR"
     gene            complement(6626..7429)
                     /locus_tag="VIF08_00705"
     CDS             complement(6626..7429)
                     /locus_tag="VIF08_00705"
                     /inference="COORDINATES: protein motif:HMM:NF040399.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEY8134531.1"
                     /translation="MTLAPAGATLQAAAPPRVASAPVPGPILFARYAFGPNRLGYCGP
                     DDAAELFGQATAGGELPALRRLAEQFEGAYPYLELIARANGLRDPLDSRVVEAYWLGS
                     DLLERVRPGDLAGSLDVRFRKRIRGDGWRWLADKPRSGALPVHAFHVLDVFPRLGLLR
                     SGALDDVVKVIDSCRVRWGRVLERDGDWLVTSVIPMALEEGKLRLGPPRVETVRGWQD
                     GRGFLGADVAPNDIISIHWDWACEVLDARRLSLLRGWTSRELEIANLTI"
     gene            complement(7426..8331)
                     /locus_tag="VIF08_00710"
     CDS             complement(7426..8331)
                     /locus_tag="VIF08_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8134532.1"
                     /translation="MNRTTKGIAFALATALISGVAIFVNSYAVKEITDAAVFTTLKNG
                     VAALALIGLTVLATRGRRSLPAGARTWSGMTIVGIFGGGVAFLLFFSGLAMASAPSAA
                     FIHKTLFVWVAMLAVPFLGERLGLIQVGALAALFGSQLLITPPTGVTWGMGETLIAAA
                     TLIWAVEIVLAKRLLARVDPLVLGVGRLGIGLIVLVGYVGITGKLGALFALDVTQWVW
                     VVGTGALLAGYVGTWFAALRFAPASVVASVLVLGAPITATLAAVANGTLPQAQPLAGY
                     LLALIAAGTVALVATRRPKAQALST"
     gene            complement(8328..8549)
                     /locus_tag="VIF08_00715"
     CDS             complement(8328..8549)
                     /locus_tag="VIF08_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEY8134533.1"
                     /translation="MCLDFPGRVVARTGDSVTVESDGRHRKASTLLYPDLVVGDWVYI
                     AAGTVVEVLDDASAKQITHEIAIARGVTP"
     gene            complement(8554..8991)
                     /locus_tag="VIF08_00720"
     CDS             complement(8554..8991)
                     /locus_tag="VIF08_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEY8134534.1"
                     /translation="MTTEPPAGVWLLACGNPDRRDDGAALVATRGLAATECGQLDIDD
                     LLRAPAGVPIVICDAAAGVAPGLVVTKTFDELLANPRGPSPHSSHALPIDQVIGVARA
                     LSNKPIDGLFVGVGGADFGFGRGLSPSVEAALPQFRAAIESLV"
     gene            complement(8988..10328)
                     /locus_tag="VIF08_00725"
     CDS             complement(8988..10328)
                     /locus_tag="VIF08_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY8134535.1"
                     /translation="MTHGMAHDPAHDAAPGEHVFDVGMLTRVEGEGSLTLRVRDGAVV
                     QAQLGIFEAPRYFERLVVGRTPDEVIDIVARICGICPVAYQMSAVHAFEALFGVQVDP
                     GVRALRRLFYCGEWIESHALHIYLLHAPDFLGYPSAIELARDHRAVVERALAMKRTGN
                     ALVARLGARPIHPLTVRVGGWSRIPTRKELLALRPALDEALATAQATVDFVTTLSAPD
                     FMREPRLVSLRHPDEYPMNEGRIVSTDGLDIALGSWREAFAEDQVAWSNALQARTHAG
                     EVYLLGPSSRVTLNAARLHPLAADALARTGLAEQIRVNPYWSIAARAVELVHATAEAI
                     DIIDSYVAPDRAFTPWEPRAGSAGWATEAPRGLLFHHYEIDERGLVSAAQIVPPTSQN
                     QAAIEADLTAFAPSVLALPHAEATLRLEQLIRSYDPCISCATHFLDLRIETADR"
     gene            complement(10325..>10948)
                     /locus_tag="VIF08_00730"
     CDS             complement(10325..>10948)
                     /locus_tag="VIF08_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134536.1"
                     /translation="QGPFDVLLVEGSISTPEQAHEIVQLRREATMLVTIGACATTGGI
                     QALRNLGDHDAFRNAVYPRPEFISSLATSSPVSEHVAVDAELVGCPISGAQLLELLTA
                     LTTGRRPQLPDEAVCAVCKRLGNPCILVATGEPCLGPVTRTGCGALCPSFGRGCYGCF
                     GPKEAANTVSLAAWFGALGRSDEQVAREFAKFNVNAPPFRAIVEAKR"
BASE COUNT         1775 a         3662 c         3863 g         1648 t
ORIGIN      
        1 ggcctcctgg tcgagctcga caagtacgtt gcccccggtt cgcggctgat ggtcatcgcg
       61 cagggccatg gcaccgacct ggcgggtgag gtcgaggccg tgggccggcg gctcaagaac
      121 ctggagctga cctaccgcgc ggcggacacg acggaccggg cggtgctcga cgaggtcacg
      181 tccgacgcct acgggcacgt cgtggtgatg tcgtactcgg acgactacga cgagcagcgc
      241 gcagatgcca agacgctggt gaccctgctc cacctgcgcg acatcgaatc aaatcggggc
      301 gagagcttca cgatcgtcag cgagatgctc gatgtgcgca atcgggcgct tgccgcggtc
      361 acccgggccg acgacttcat cgtcagcggc aagctcgtga gcctgatgat gtcgcagctc
      421 gcggagacgc ctgacctgcg ccccatcttc gatgacatgt tcgacgagga cggctcggag
      481 atctatctca agcctgccgg cgactacgtc aagctcggcg cgccggtcga cttctacacc
      541 gttgttgagt cggcccggcg gcgcggccag atcgcctttg gctaccgatt gctcggccag
      601 gccaacgatc cgaataagaa ctacggcgtc gcgatgaatc ccgacaaggc cgagcaggtc
      661 accttcggca gcgacgacaa gctgatcgtc atcgcggagg attaggaccc cgacgttcag
      721 ctccgcagcc ggcctccggg ccgctgctgc gcgctcggca cggcctgacg ctgaagcgtc
      781 tggccgtccc tacgcttgcg gcgggctacc tgcgcccggt cagccaccac gtggtcatcg
      841 ggcccttgcc cttcacctcg acctggccgc gcggctcgaa ttcgaacgtg tccttcaggc
      901 gctcgtagac gagcggcgtc acctggatgc ggcccggctc accgagcgat tccatgcggc
      961 tcgccacgtt gactgtgtcg ccccagacgt catagatcgt tcgcttctgg ccgatgacgc
     1021 ccgcgaccac tggcccggcg ttgatgccaa tgcgcattcg gattgctccg tggtcgccgc
     1081 tctgcagctc gcgcaccgcg agggccatgt ccagcgcaaa gccggcgaga cgctcgagat
     1141 gattggggtc ggcgatgggc acgccgccta tgaccatgta ggcgtcgccg atcgtcttga
     1201 ccttctccag ctcatagcgc gcgacgatga cgtcgagcac gctgaagatg tcgttgagca
     1261 tctccaccag atcgtccgcg gagaggcggc tcgacagctc ggtgaagccg acgatgtcgg
     1321 ccatcagtag tgatgcgtcc tcgaaacgat cggcgatgac gcgctcaccg gagcggagcc
     1381 ggttggcgat gggcgggggc aggatgttca gcagcagccg ctcggtctct tccttggcgc
     1441 gcgccacctg gcgcgcggcg gtcacatcgg tgccgtacag attgatgaac ccgaactcgg
     1501 ggatctcgac cgcggtcagc tcgtagaact ggtcgccggc gatgcactcg atgggttcgc
     1561 gtgaacgcgt ggcgacgcgc gccgtaatct gggcctggag ctcgcccggc agcggctcgc
     1621 cgacggcgaa tttcagggcg gcgcgcatcg gcgcgctggc gtcgttggcg tagatcagga
     1681 cgccgctgga atcggtgcgc agaacgggat tcgggttctg acccggaaac ttggccacca
     1741 ccttctcggc cgttatgtcc gtgccgtaga cgttgatgaa gccgaactcg gggatgcgca
     1801 cgggacgcag ggcgtaggtc cgatagtcgg cctccagctc gagcaggtcg cgcgtgggcg
     1861 cgtcggccgc ggcgaggacc cgggccgcga tctccattgg ccacggatca ccgactttca
     1921 ggccaaaggc ctcgatgagc ggctggctcg cagcgttggc gtagatgagc gtgcccgagt
     1981 cgtccacgcg aaagaccggg tttggattct ggttgggaaa gcgctcgacg accttctccg
     2041 cggtgatgtc cgtgccgtag aggttcatga accccagttc gggcaccggc acgggcagga
     2101 tggcgaagat gcgcacgccg tcgcgcagct cgatcgagcc gcctgcgctg gcggcggcgc
     2161 gcagccgtgc cagcagctcc ggctccatcg tacggccaac cgccgcgttc atcgcccgca
     2221 aaaccggcgc gctcgagagg ttggcgtagg tcagctggcc gtcgtcggcc acgcgcagca
     2281 cggggtgcgg gttctgatcc gggaagcggt tgatcgcctg gatctggctc tcgaccagac
     2341 cgtgatcgcg atcgcgatgg tcgccgctcg acgtttcgcc gggaagcggc cgctcgctca
     2401 tgagacgggc agtctaggcg ccgtccagca gatgccgcag ctcttcccgg gcttcctcga
     2461 ctcgcgcccg cttgcccgag atcacctcgg gtggaagggt ctgtgtcgcc gacagatcgg
     2521 cgtacaggga acgcagctcg atcacgagct tgcgcaccct ctcgtgcttt ggatcagcgg
     2581 gcggcacgac ttccagggcg cgctcggctt cgcgccacac cttcaacgtg tcaacgatca
     2641 gtgtctgcac ggtgaactcc atcccccaag ttcaacgagc cgccggcccc tcagccggcc
     2701 cgccgcgata cacggcagcg gaatcctagc gcgtgactca atagggctgc aacgaccctc
     2761 tcgtgaatca gcccagccgg gccagcagcc tgccgtctac ccagccggtc cacccgttgg
     2821 agcccatgac gcgcgcccat gctccgcgct cctccaggac gctgagctgc acccctccgg
     2881 cgagagtcac cattggcgga cggctgggat cgggcgtctc ccacgcggcc aggccggtcg
     2941 gcggggcgac gtgcgttggc gtccaggcgc cgggcgcggc gatggcgggt gccgccacgg
     3001 cggcagcaac cggttgagca gcggtgatgt caggcagcgc gttcagctgg gcgcggatcg
     3061 tgcggatgcg ctcgcgctgc gccaccatgc tgggatcgtc atccgatccg gtgcgtgagc
     3121 ggatcatcgc caggttgatc tgctcgcgtt gcagctgagc ggccagcttg cctccctgcg
     3181 ggcccttgat tgcgcgcacg cggcgcctgg ccgcccacct gctgcgcagg ccgccgagct
     3241 cgttgagctc cggctgagtg agcacgtcgc tgcccacgtc ggcctgggtc agggtggcga
     3301 accagcgttg ctcgcgccgt cccgccagga tgaccaggaa gatgaggaag ccgaggaacg
     3361 gcaggccctt gatcaggccg aagacgacga tgccaccgat gtctgtcagt aggccgttca
     3421 gcagtggcga gttccagagc atgtgcgcca cgacgccggc caggaacagg ccaacggcga
     3481 ccatcaggcg gcgctgataa ggctgatcga gccgagtcac gaagtaggcc aggccggcgc
     3541 ccgtcaaccc cgtccacatc acgtgcatgt aggcaccgat caagagcgag cgcaggaaga
     3601 acatgcccag gaccggcccg atctgatcgg cgccgcctga gttggcgacc gcctggatga
     3661 agtactggac attctcgacc gccgcgaagc caagaccgac cacggcgcca tacacgaatc
     3721 cgtcgagcac gtcatcgatc tcgttgcggg caatgagcag gatcgtcacc acgcccaggt
     3781 atttgagggt ctcctcgacc ggcggcggca cgatcgccac gccccacgtt tgagcgaagc
     3841 tcggcccgaa tagcttggcc accacctcga tcagcgacgt gttgatcgtc accgccagtc
     3901 cggtggcgat cacaccgccc catacgaacg ccgcgagcac gagcgagatg ggctcgcgct
     3961 cgtagaggtc gagcagccag atggcgatgc cgacgggtac gacgtagaga gcgagcagcg
     4021 cgacgctgaa gatccagccc gacgggaaga actgcaggta acgaagctgc tcggtcagga
     4081 tggtcagccc ggccaccgcg aacaggacga cgaacagcca gaacgcgggc tggcgaacct
     4141 gggtgaagcc agtttggacg ccccagcggg gtagcggcgg agccgcggcg accgaaccgg
     4201 cgttcatggt tgcacctgcc ggcggcgggg gtggcgtcgg tgcgctgggc ggcggggccg
     4261 cgacggacgc cttcttgttc tggcggcgct cagggctcga atcactcatc tggctactgc
     4321 accacctgga ggttgtcgat cattcgctgg gcttccggca agagctggcc cagggtgccc
     4381 gcgctgcccc aggcgtcgaa gatgaagccg ttggccccga ccacgatggt cgtcacctgg
     4441 ccctcgatct gactctggcc aaagaccccg gtgtacgttc ctcttgcggc cggaatccca
     4501 ttgccgatct ggagcaggtt gggctgtgag gcgttgaagc cggtcgcggc aggctggagg
     4561 ctctgtgtca gataggcgtt gtagacggcg cccgcgtcag cctggccctg gatcgacgca
     4621 ccgtagagat cgaccgcgac gttgcccttg acgagccgga cgatgacatt gctgtccggc
     4681 acctcctgcg gcgtccagcc gttctcgagc gggatgcgca gcggtcccag cgtgagagtt
     4741 gaaccgggct gggcgggcgc gctcgttggg cccggtccaa ccggaccgtt ggtcggccct
     4801 gggacggtcg ttgggaccgg ccccggcccg ctgggtccct ggccgccggg gccgggaatg
     4861 ccggggccgg cgccgccctg ggggatcacc gcgttcacga tctgggcccc gaagtacagc
     4921 gcgatgatga tcccggccac gatcgccgtc ggccgaatgc cggccgggtc gagctgcgag
     4981 aacagggccc gcgcctttgc cccggcgtca atgcccggcg gcggcggcgg aggcggcggc
     5041 gcagccagcg caggctgcac ggccgggtcc ggcggcggcg gggccacggg cgattgcgga
     5101 ggcggcggtg gttcgctgct cacagggcgg gactctagca gccgttacaa gtggttcgga
     5161 agggttagcg acactttgcc catcagcgcc gctccaccag gcgcgctgtc gtcgttgtcc
     5221 cagcgttggc cgcctggatg gtgaactcga ccagcatgcc cgtcgactgc tcgtacgtga
     5281 agcgggcgaa gttgccgggc agttgaacgt cgatgctcac gacggccgtg cccgcctgat
     5341 cctggccgac cgccgagacc gtggttcgct cgccggtcag gccatcctgg tccagtacct
     5401 ggccggcgct catccccgcg agcgaggcag ggtcgtacca atacgggccg tttggaccgg
     5461 cgacgccctg gctctgggtg ttgatgttga gcgccggtgt ctcagtgcgc gacgtgaacg
     5521 atgaccaggt ggcgcccccc tggccgagct gcaccgtgac ctgggtcgga taggtcagat
     5581 tggcgctcga tggatccacc gggttcgtga agttgtacgt gccctggtag atcagctgcg
     5641 aagtgccgtt gacccagtct ggcttgggtg cggtcatgcc cggcaggttg cgcgtccgga
     5701 agccggcgaa gcgggccatc gtgagctgac tgttgctcgt cggcgcaccc tggcccggcg
     5761 cgctgagcgg cgacgtgatc ccaccggtgc tggtattggc cgacaacaga atgccggtgg
     5821 cgaggtcata ggtgtacgac gagtaagtgc tcgtgcccag cccgctcacc gcggacaaag
     5881 cgtcgaaggt ttgcgtgccg agtgagtagc gaccgcgcaa taccagcagg tcgcccaggc
     5941 cgctctgggc gatctgcgcc aggcgcgctg ggtggatcca cgctccatcg acatcgatcg
     6001 cgctgacact ggcgccgcca acgggcgtcc agatgagcgt gttgttctcg cggaaaaagg
     6061 tgtaaagcgt gttcgagatt gccgcacggc tgccgtcgag cgcgacgatg tcgagctgtg
     6121 acagcccatc gccgctcgcg gtcccctggc cctcgccgct ctcgtcggtg cggcggtagc
     6181 acttgcccgt cttgggatca gtccagttat taccggtaca cggctcctcg acccatgaga
     6241 agcggctgtt ggcgaccgac gccgccgcgg cataccaagt gagccgcgcc ccggtggcca
     6301 cgaactgagg cggagagtcg agagacccgg gatcctgcgg cactccgggt tggccgggac
     6361 cgaagggcgg agtgggcccg accacaggcg cgttagggtc ggccggcccc gtcgccgcgc
     6421 cgggctggaa gagcaggatc ggcagcagga ggatcgagac ggcgacaagc gccagcgcgc
     6481 cgactgcgcc gtacagcgtc caatccgctc gcggattcac cgcagcttcc tccgctcagg
     6541 cgcgtcgcgc cacatggccg gcgattgtga caggcgctga tggtgcgcgc cagagggtgg
     6601 cgcagcgcgg gcgggtagag agtcgctaga tcgtcaggtt ggcgatctcg agctcacgcg
     6661 acgtccagcc gcgcagcagg ctcaagcgac gcgcgtcaag gacttcgcac gcccagtccc
     6721 agtggatcga aatgatgtcg ttgggcgcca cgtctgcgcc caggaagcca cggccgtcct
     6781 gccagccgcg cacggtctcg acgcgcggtg ggccaaggcg cagcttgccc tcttcaagcg
     6841 ccatcgggat gacggacgtg accagccagt caccgtcgcg ctcgagcacg cgtccccaac
     6901 gaacgcggca cgagtcgatc accttgacca cgtcgtccag cgccccggaa cgcaacaggc
     6961 caaggcgcgg gaagacatcg agcacgtgga aggcgtgcac cggcagcgcg ccggagcgcg
     7021 gcttgtcggc cagccagcgc cagccgtcgc cgcgtatgcg cttgcggaaa cggacgtcga
     7081 gcgagcccgc cagatccccg ggccggacgc gctcgaggag atcgctgccc agccagtacg
     7141 cctcgaccac ccgggagtcg agcggatccc gcagcccgtt cgcgcgcgcg atcaactcga
     7201 ggtacggata ggcgccctcg aactgctcgg ccagccggcg cagagcgggc agctcgccac
     7261 cggccgttgc ctggccgaag agctcggccg cgtcatccgg cccgcagtag cccagccggt
     7321 tcggaccgaa cgcgtagcgc gcgaacagga tgggccccgg gaccggagcc gaggcgacgc
     7381 gcggtggcgc ggcagcttgg agcgtcgcgc ccgccggagc cagcgtcatg tcgacagcgc
     7441 ttgcgccttc ggccggcggg tggcgacgag agcaaccgtc ccggcggcga tcagcgccag
     7501 cagatacccc gcgagcggct gcgcctgcgg cagggtaccg ttggcgaccg cggcgagtgt
     7561 ggccgtgatg ggcgcgccca ggacgagcac gctggccacc acgctggccg gtgcgaaccg
     7621 caatgccgcg aaccaggttc cgacgtagcc cgccagcagg gcaccagtgc ccacgaccca
     7681 cacccactgc gtgacgtcga gtgcgaacag cgcgccgagc ttgccggtga tgcccacata
     7741 gccgaccagg acgatcaagc cgatgcccag ccggccgaca cccagcacca gcgggtctac
     7801 ccgcgccagc aggcgcttgg cgagaacgat ttccacagcc cagatgagcg tggccgcggc
     7861 gatcagggtc tcgcccatgc cccaggtgac gccggtgggc ggtgtgatga ggagctggct
     7921 gccgaacagc gcggccagcg cgccaacctg gatgagaccg aggcgctccc caaggaaggg
     7981 cacggccagc attgccaccc agacaaagag cgtcttgtgg atgaacgcgg cggatggcgc
     8041 gctcgccatc gcgaggccgc tgaagaagag caggaacgcg actccgccac caaagatgcc
     8101 gacgatcgtc atgcccgacc acgtccgcgc gccagccggc agcgatcggc ggccgcgcgt
     8161 cgcgaggacg gtcaggccga tgagcgcgag cgcggcgacg ccgttcttga gcgtcgtgaa
     8221 cacggccgcg tcggtgatct ccttgacggc gtacgagttg acgaagatgg ccacgccgct
     8281 gatgagcgcc gtagcgaggg cgaaggcgat gccttttgtt gtccggttca cggtgttacc
     8341 cctcgtgcta ttgcgatttc atgggtgatc tgtttggcgg aggcgtcgtc cagcacttcg
     8401 acgacggtgc cggcggcgat gtagacccag tcaccgacga ccaggtcggg atacagcagc
     8461 gttgatgcct tccggtgccg gccgtcgctc tcgacggtca cggagtcgcc cgtccgtgcg
     8521 acgacgcggc cgggaaagtc gaggcacatc tggtcagacc aggctttcga tggccgcgcg
     8581 gaactgtggc agggccgcct caacggaagg cgacaggccg cggccaaagc cgaagtcggc
     8641 accgccgacg cccacgaaga gcccgtcgat gggtttgttc gacagcgcgc gggcaacccc
     8701 gatgacctgg tcgatgggca gggcgtggct gctgtgcggc gacgggccgc gcgggttggc
     8761 gagcagctcg tcgaaggtct tggtcacgac caggcccggc gcaactcccg cggcggcatc
     8821 gcagatgacg atcggcacac cggctggcgc gcgaaggagg tcgtcgatgt cgagctggcc
     8881 gcactcggtc gccgccaggc cgcgcgtcgc cacgagggcc gcgccgtcgt cgcgccgatc
     8941 ggggttgccg caggccagca gccagactcc tgcaggaggc tccgtggtca tcggtcggcc
     9001 gtctcgatgc gcagatcgag gaagtgcgtg gcgcacgaga tgcatgggtc gtatgagcgg
     9061 atgagctgct ccagccgcaa cgtcgcctcg gcgtggggca gcgccaggac ggatggggcg
     9121 aaggcggtca gatcggcctc gatggcggcc tggttctggc tggtgggcgg cacgatctga
     9181 gccgcactga ccaggccgcg ctcgtcgatc tcgtagtggt ggaagagcag gccgcgcggc
     9241 gcttcagtcg cccagccagc cgacccggcg cgcggctccc acggtgtgaa ggcgcggtcc
     9301 ggcgcgacgt aactgtcgat gatgtcgatc gcctccgccg tggcgtggac gagctcgacc
     9361 gcgcgcgccg cgatgctcca gtacgggttg acgcggatct gctcggcgag cccggtgcgc
     9421 gccagggcgt ctgccgcgag cggatggagc cgcgcggcgt tgagcgtgac gcgcgacgac
     9481 ggacccagca ggtacacctc gcccgcgtgg gtgcgcgcct ggagcgcgtt cgaccacgcg
     9541 acctggtctt cggcgaacgc ctcgcgccac gagccgaggg ctatgtccag gccgtcggtg
     9601 gagacgatcc ggccctcgtt catcgggtac tcgtcgggat gacgcagtga gacgaggcgc
     9661 ggttcgcgca tgaagtcggg cgccgagagc gtcgtcacga agtcgaccgt tgcctgggca
     9721 gtggccaggg cctcgtccag cgccggccgg agtgccagca gctctttgcg cgtgggtatg
     9781 cgcgaccaac cgccaacgcg gaccgtcagc gggtggatgg gccgcgcgcc cagccgcgcg
     9841 acgagcgcgt tgccggtgcg cttcatggcc agcgcgcgtt cgacgaccgc gcgatggtcg
     9901 cgcgccagct cgatcgctga cgggtagccc aggaagtcgg gcgcgtgcag caggtagatg
     9961 tgcagcgcgt ggctttcgat ccactcgccg cagtagaaga gccggcgcag cgcgcgcacc
    10021 ccgggatcga cctggacacc gaacagtgcc tcgaacgcgt gcacggcgct catctggtac
    10081 gcgaccgggc agatgccgca gatgcgcgcc acgatgtcga tcacctcgtc cggcgtgcgg
    10141 ccaacgacca ggcgctcgaa gtagcgcggc gcctcgaaga tgcccagctg cgcctggaca
    10201 accgcgccgt cgcggacgcg caacgtgagc gagccctcgc cctcgacgcg cgtcagcatc
    10261 cccacgtcaa agacgtgttc accgggcgcg gcgtcgtgcg ccgggtcgtg cgccatgccg
    10321 tgcgtcatcg cttcgcctcc acgatcgcgc ggaacggcgg cgcattgacg ttgaacttgg
    10381 cgaactcgcg cgcaacctgc tcgtcactcc gtcccagcgc gccgaaccac gccgcgagcg
    10441 agaccgtgtt ggcggcctcc ttgggaccga agcagccgta gcagccgcgg ccaaaggacg
    10501 ggcacagggc gccgcagccg gtgcgcgtca ccgggcccag gcacggctca ccggtcgcga
    10561 ccaggatgca cgggttgccc agccgcttgc acaccgcgca caccgcctcg tcgggtagct
    10621 gcggccgccg tccggtggtc agcgcggtca acagctcgag cagctgcgcg ccgctgatcg
    10681 ggcagcccac cagctcggcg tcaaccgcga cgtgctccga taccggagac gacgtggcga
    10741 gcgaggaaat gaattcgggc ctgggataga ccgcgttccg gaacgcgtcg tgatcgccca
    10801 ggttgcgcag cgcctggatg ccaccggtcg tggcgcaggc gccgatcgtg acgagcatcg
    10861 ttgcctcgcg gcgcagctgg acgatctcgt gcgcctgctc gggggtgctg atcgagccct
    10921 cgaccaggag cacatcgaag gggccctg
//
LOCUS       DATBRX010000021         7438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1682103, whole genome shotgun sequence.
ACCESSION   DATBRX010000021 DATBRX010000000
VERSION     DATBRX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682103"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VIF08_00735"
     CDS             complement(<1..1206)
                     /locus_tag="VIF08_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY8134537.1"
                     /translation="MSSDKPWLHGRKPGDRRVRIERPHAEFFRYAAPGQFVAREAASR
                     PRTALGRFIERGRHALLGRPLSIHEEMDQRLSKKKALAIFSSDAISSSAYATEEILRV
                     LVLAGAGALLLSLPIAMGIALLLAVVAISYRQEIYAYPSGGGDYAVARANLPGIFALV
                     VAGALLVDYVMTVAVSTSSAVEQVISALPGLADIRVEIAAVAILLIMLGNLRGLRESG
                     NIFAVPTYIFVFSTLAIIAIGVFRIVVLGESAKPPEPLPGAADPLQLVGIVLIIKAFA
                     SGSVALTGTEAIANGVPSFKPPEARNAATTLLVMAVLLGVLFIGLTFVADSFGIVPVD
                     HPAVKTVISQVAAKIYGDNTLLFYLYQAFTALILFLAANTSFNAFPRLAAILARDGFM
                     PRVFSFRGDR"
     gene            complement(1207..1929)
                     /locus_tag="VIF08_00740"
     CDS             complement(1207..1929)
                     /locus_tag="VIF08_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8134538.1"
                     /translation="MRIAVLSDIHGNLPALEAVLAALKPYDAVWELGDIVGYGPQPDE
                     VVALLTAEGATGVRGNHDSAAIGLLDTDAFNDDARTAVEWTADRIEPRTRDWLAALPL
                     TSTDGPFTLVHGSPRDPTWEYVYSAGVARANMPLFETAYCLVGHTHVPLVFRQRGSKV
                     EGVGVREGTPLALGSERLVINPGSVGQPRDGDPRASALLVDTDAASLEWHRVDYDIGQ
                     VQELMERAGLPRRLSARLQFGL"
     gene            complement(1937..2788)
                     /gene="rsmG"
                     /locus_tag="VIF08_00745"
     CDS             complement(1937..2788)
                     /gene="rsmG"
                     /locus_tag="VIF08_00745"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEY8134539.1"
                     /translation="MDARDRSSLPRRASPPRRAALPQAVDATAELDPIFSEVVEHGLE
                     ELGLTLDVAARDAIAGHVRLLLAWNEHINLSGLRTAEQIARGHVLDALLAVGVLRDLT
                     GDRPRLLDLGSGGGFPGLPLAIALPTGRTALVDSIGKKAAFLRVAAAAVTAVAQVDVL
                     AERAEDLAEEHVHRGAWDVVTARAVGSVAEVAELGLPLVKVGGHVVAWKSAGTVEPAA
                     TLKSEVADAARLIRSTGGDRPRIVALPAAARIGLPGRCLVVIDKVRPTPDGYPRVPGE
                     RRRRPLG"
     gene            complement(3301..3651)
                     /locus_tag="VIF08_00750"
     CDS             complement(3301..3651)
                     /locus_tag="VIF08_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEY8134540.1"
                     /translation="MSTPRSGQSAASSGQPSRHSYTKDRAQLLRRLSRMEGQIRGVAR
                     MIEREEYCIDILQQTAALRAAVDAVSLLLMEDHVAGCLATAVKTGKADAYTEEVMEVV
                     RRSMGRPIRAKATD"
     gene            3727..4125
                     /locus_tag="VIF08_00755"
     CDS             3727..4125
                     /locus_tag="VIF08_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134541.1"
                     /translation="MDSPDWLRTQLTTFICPACGRRYRGSKMRLLAEREGLFFVDLDC
                     SRCGSHTVAIVTVEVDDSDFTIEVSDVVGEDEIEHLGEPLPRGAAPVTADDVLEMHEF
                     LNRYRGSVDELLQTGTAGSGGQRTDGAERR"
     gene            4122..4646
                     /locus_tag="VIF08_00760"
     CDS             4122..4646
                     /locus_tag="VIF08_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134542.1"
                     /translation="MTSAPRFAHASEAELARILDYYGVAWQYEPRTFPIQWNPDGKVI
                     ESFSPDFYLPELELYVELTTLKQSLVRRKNRKLRRLRELYPDVRVKLFYGKDFRALML
                     KYGRVDLVASLSGLEGQIMPGPAPVPELELEDVALQTHAGPAVVARPSRPRVSRGSRR
                     RRGRRAYLAARSAA"
     gene            4670..5242
                     /gene="hpt"
                     /locus_tag="VIF08_00765"
     CDS             4670..5242
                     /gene="hpt"
                     /locus_tag="VIF08_00765"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY8134543.1"
                     /translation="MTTELRVPDLRTDIGEVLITEEQIRAKVRELGSQISADYAGKSV
                     TLVSVLKGSLPFMADLMRAIQIPVQIDLMEVSSYGGATTESSGLVRILKDLSSTIAGK
                     DVLIVEDIIDTGLTLNYLLRYLRGKGPNSLRICALLDKPARRLVEIPIDYTGFVIPDE
                     FVVGYGLDFGEYYRNVPFIGVLRRELYEPG"
     gene            5239..5658
                     /locus_tag="VIF08_00770"
     CDS             5239..5658
                     /locus_tag="VIF08_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134544.1"
                     /translation="MTRRALGRGRFLVVIGALVALAGIIPSWWTRPLTGQDALSGNGL
                     EGAGIIIFLAVLALIAVVVLPYAARDGDSALDRPASYVLLALMAVGAFLFRIFEISRF
                     ASLGLPMEAPGMWISGLGLLIVVWGVADLLTERPTTY"
     gene            complement(5652..6761)
                     /locus_tag="VIF08_00775"
     CDS             complement(5652..6761)
                     /locus_tag="VIF08_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8134545.1"
                     /translation="MPDQSRAIPLAQASTLPARYYLEPGYLEREKEQVFGRTWQLVAR
                     HEDLARPGDFVPTTILDEPIVITHGTDGVLRGFYNVCRHRAGQVALSKGNRRSLQCRY
                     HGWTYGLDGTLRACPEMEETEDFRKEDFGLTPVRVERWGPFVFANLDDAAPPLSEVLG
                     AIPDEVARAGFEVDHMRLVERREYLIECNWKVYVDNYLEGYHLPIAHPDLFRELDYDA
                     YRVETQRYYSKQHAPIRELKEGQEIGRDRRFVRTADGEEDALYYWLFPNTMFNIYPDN
                     MSSNVILPVGHDRTLTIFEWFFAEPGSGAGWESMQQGIAFSDQIQQEDIVLCEQVQRG
                     LRSRAYDTGRFSAKRENGVYHFQSLVREFLGEDSQ"
     gene            6848..>7438
                     /locus_tag="VIF08_00780"
     CDS             6848..>7438
                     /locus_tag="VIF08_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY8134546.1"
                     /translation="MIPRRAAFIVAATLIFAAACAGPAASPTPVPTPTSAPSAASTAV
                     CDNLPAGSEGDLLATICAAGKIVISTDADYAPQSSVTPSGGYEGFDIDVGEEIASRLG
                     VDAEWSAQMWDTVIAGSWSGRWDMSVGSITVCCDREQLFDFTQPYYFTPAQVAVLEGS
                     GFASIDDIAGETVCVGEDTTYLYWVEGTLELAAPNRI"
BASE COUNT         1211 a         2488 c         2515 g         1224 t
ORIGIN      
        1 gcggtcgccg cggaacgaga agacgcgcgg catgaaaccg tcgcgcgcca ggatggcggc
       61 caggcgcggg aaggcgttga aagacgtgtt cgcggccagg aagaggatca gcgccgtgaa
      121 cgcctggtag aggtagaaca gcagcgtgtt gtcgccgtag atcttggccg ctacctggct
      181 gatcacggtc tttacggccg ggtggtcgac gggcacgatc ccgaacgagt cggcgacgaa
      241 cgtgaggcca atgaacagca cgccgagcag cacggccatg acgagcagcg tggtggcggc
      301 gttgcgcgcc tccggcggtt tgaacgacgg cacgccgttg gcgatcgcct ctgtgcccgt
      361 cagcgccacg gagcccgacg cgaaggcctt gatgatcagc acaatgccga ccagctggag
      421 tggatccgcg gcgccgggta gcggctcggg cggcttcgcg ctctcgccta ggacgacgat
      481 gcggaagacg ccgatggcga tgatcgccag cgtggagaag acgaagatgt aggtcgggac
      541 ggcgaagatg ttgcccgact cgcgcagccc gcgcaggttg cccaacatga tcagcaggat
      601 cgcgaccgcg gcgatctcca cgcggatgtc ggcgaggccg ggcagtgccg agatcacctg
      661 ctcgacggcc gacgaggtcg acaccgcaac cgtcatcacg tagtcgacca ggagcgcgcc
      721 cgccacgacg agcgcgaaga tgcccggcag gttggcccgc gcgacggcgt aatcgccgcc
      781 acccgatgga taggcgtaga tctcctggcg gtagctgatc gccacaacag ccaagagcag
      841 cgcgatgccc atcgcgatag gcaggctgag caggagcgcg ccggcgccgg cgagtacgag
      901 gacgcgcagg atctcctcgg tggcgtatgc cgacgagctg atggcgtcgg agctgaatat
      961 cgccagcgcc ttcttctttg agagccgctg gtccatctcc tcgtggatcg aaaggggccg
     1021 gcccagcagc gcgtggcggc cgcgctcgat gaagcggcca agtgccgtcc tggggcgtga
     1081 cgcggcctca cgcgcgacga actggccggg tgccgcgtag cggaagaact cggcgtgggg
     1141 gcgctcgatg cggacacggc ggtcgcccgg tttgcggccg tgcagccagg gcttgtcgct
     1201 gctcatctag aggccgaact gtagccgcgc gctgagccta cgcgggagac cggctcgctc
     1261 catcagctcc tggacctggc cgatgtcgta gtcgacgcgg tgccactcca gcgacgcggc
     1321 atccgtatcc acgagcagcg ccgacgcgcg cgggtcgccg tcgcgcggct ggcctacgct
     1381 gcccggattg attacgagcc gctcactgcc gagcgcaaga ggggtgccct cccggacgcc
     1441 cacgccctcg accttgctgc cgcgctggcg aaagacgagc ggcacgtggg tgtggccgac
     1501 caggcagtac gccgtctcga acaggggcat gttggcgcgc gccacgccgg cgctgtagac
     1561 gtactcccag gtcgggtcgc gcgggctgcc atggacgagc gtgaatggcc catcggtgct
     1621 ggtcagcggc agcgccgcca gccagtcgcg cgtccgtggt tcgattcgat cggccgtcca
     1681 ctcaaccgct gtgcgcgcgt cgtcgttgaa cgcgtcggtg tcgagcaggc cgatcgccgc
     1741 ggagtcgtga ttgccgcgca cgcccgtggc gccctcggcg gtcaggagcg ccacgacctc
     1801 gtcgggttgc gggccatagc cgacgatgtc gcccaactcc cacacggcgt cgtagggctt
     1861 gagcgcggcg agcacggctt cgagtgcggg caggttgccg tggatatccg agagcacggc
     1921 aattcgcatg gatgaatcag cctagcgggc ggcgccgccg ctcgcctggc acgcgtgggt
     1981 agccgtccgg ggttggcctg accttgtcga tcacgacgag acaccgaccg ggcaggccga
     2041 ttcttgccgc ggccggcagc gcgacgatgc gcggcctgtc accgccggtt gatcggatga
     2101 gccgggcggc atcggcaacc tccgacttga gcgttgcagc cggctcgacc gtaccggccg
     2161 atttccaggc caccacgtga ccgccgacct tcaccagcgg caggcccagc tccgccacct
     2221 cggcgacgga gccaacggcg cgcgccgtga cgacgtccca tgccccgcgg tggacgtgct
     2281 cctcagccag atcctcggcg cgctctgcaa gcacatccac ctgggccacg gccgtgacgg
     2341 cggccgcggc aaccctgaga aacgcggcct tcttgccgat cgaatcgacg agcgccgtcc
     2401 ggcccgttgg cagcgcgatc gcgagtggca ggccggggaa gccgccgccg ctgcccaggt
     2461 ccagcagtcg cggcctgtcg cccgtgagat cgcggagcac cccgaccgcg agcagcgcgt
     2521 cgagtacgtg cccgcgcgcg atctgttcag cggtgcgcag accggacagg ttgatgtgct
     2581 cgttccaggc gagcagcagg cgcacgtggc ccgcgatcgc gtcgcgcgcc gcgacgtcca
     2641 gcgtcaggcc cagctcctcc agcccgtgct cgaccacctc gctgaagatc gggtcgagct
     2701 cggccgtcgc gtcgaccgcc tgtgggagcg cggctcgtcg agggggagag gcacgccgcg
     2761 ggagcgagga ccgatcacga gcgtccatgc ccgaacgctc ctgagccgtt gtgctgtccc
     2821 cgctccccgc ggcctgacgt tctggcacca ccggcctttc agttttccct cgtcgtccgg
     2881 ctcgtccgac cgactttcat cggccgccgg gtcgtcgtgg ttaccgtact cggtctggtg
     2941 cgcctccccg ctaggacgcg ccagtgatgc aaggcgcgca gtgtcgccat cgcgtgagcg
     3001 gcgcgtcaac ggccttttcc actagttgtc ggggagtccg cgcatctgtg gagaaaacgc
     3061 gcgggcgcgg tactcgaatg tggacggtcg atcgcacgat ttggagaccg acgactagaa
     3121 gagctggacc cccagacacg acacgacggc ccagccgagc gagccggtga ggatcagcac
     3181 aacgacgaac gcgagaattc ctctacccat gcgcgaactg tgaagcgcag cgcctctcat
     3241 tcacgggcac cgaatcatgg tcgcgcatgg cgaacgcgtt gcgggaactc gttttggcgg
     3301 ctaatcagtc gccttggcgc gaatcggccg gcccattgag cggcgcacga cctccatcac
     3361 ctcttcggtg taagcgtcgg ccttgccggt cttcaccgcc gtggccaggc agccggcaac
     3421 atggtcctcc atcagcagca acgagaccgc atcaacagcc gcgcgcaatg cggccgtctg
     3481 ctgcaggatg tcgatgcagt attcctcgcg ctcgatcatc cgcgcgacgc cacggatctg
     3541 gccttccatg cgcgacaggc gacgcagcag ctgggcgcgg tccttggtgt aactgtggcg
     3601 cgagggttgg ccggacgatg ctgcgctctg tccggaacgc ggtgtactca tactggtagg
     3661 gagtataagt cagatgcgac gctgacagca ctcgtcggag cgtctttggg gctagactcg
     3721 cagcagatgg acagcccgga ctggctgcgg acgcaactca cgaccttcat ctgcccggcc
     3781 tgcggtcggc gctaccgcgg cagcaagatg cgcctcctgg ccgagcgcga ggggctcttc
     3841 ttcgtcgacc ttgactgttc gcgctgcggc agccacaccg tcgccatcgt gacggtcgag
     3901 gttgatgaca gcgacttcac gatcgaggtt tccgatgtcg tcggcgagga cgagatcgaa
     3961 cacctgggcg agccgttgcc gcgcggggcc gcgcccgtga ccgccgacga cgtgctcgag
     4021 atgcacgagt tcctgaaccg ctaccggggc agcgtggacg agctgctcca gaccggcact
     4081 gccggttccg gcggacagag aacggatggt gccgagcgcc ggtgacctca gcaccaaggt
     4141 ttgcccacgc cagcgaagcc gagctggcgc ggatcctcga ctactacggc gttgcctggc
     4201 agtacgagcc acgcaccttc cccatccagt ggaaccccga cggcaaggta atcgagtcct
     4261 tctcgcccga cttctacctg cccgagctgg agctgtacgt cgagctgacc acgctcaagc
     4321 agagcctggt gcgacgcaag aaccgcaagc tgcgccggct gcgcgagctg tacccggacg
     4381 ttcgcgtgaa gctcttctac ggcaaggatt tccgcgccct gatgctcaag tacggccggg
     4441 tggacctggt cgcgtcgctg tctggtctcg agggtcagat catgcccggg cctgctcccg
     4501 tgccggagct tgagctcgaa gatgtcgcgc tgcaaaccca cgcagggccc gcggtggtgg
     4561 cgcgcccatc acgaccgcgc gtctcccgcg gctcgcgccg acggcgtggc cggcgggcat
     4621 acctcgccgc gcgcagtgcg gcctgacccg cgctagcctt cacccataga tgaccactga
     4681 gttgcgcgtc ccggatctcc gcaccgacat cggcgaagtg ctcatcaccg aagagcagat
     4741 cagggccaag gtgcgcgagc tgggcagcca gatatcggcc gactatgccg gtaagtcggt
     4801 caccctcgtc agcgtcctca aaggctcgtt gccgttcatg gctgatctca tgcgggccat
     4861 ccagattccc gttcagatcg atctcatgga ggtcagctcg tacggcgggg cgacgaccga
     4921 atcgtcgggc ctcgtgcgca tcctgaagga cctctcctcg accatcgccg gcaaggacgt
     4981 cctgatcgtg gaggacatca tcgacaccgg gctgacgctc aactacctgc tgcgctacct
     5041 gcgcggcaag gggcccaaca gcctgcgcat ctgcgcgctg ctcgacaagc cggcgcggcg
     5101 cctggtcgag atcccgatcg actacacggg ctttgtcatc cccgatgagt tcgtggtcgg
     5161 ctacggcctc gacttcggcg agtactaccg caatgtgccg ttcatcggcg tgctgcggcg
     5221 cgagctgtac gaacccggat gactcgccgc gcgctggggc gcggccggtt cctggtcgtg
     5281 atcggcgcgc tggtagctct cgcgggcatc atcccgtcgt ggtggacgcg gccactgacc
     5341 ggccaggacg cgctctccgg taacggcctc gagggcgccg ggatcatcat cttcctcgcc
     5401 gtgctggcac tgatcgccgt cgtcgtcctg ccctacgcgg cgcgcgacgg cgactcggcg
     5461 ctcgaccggc ccgcctcgta cgtcctcctg gcactgatgg ccgtgggcgc gttcctgttc
     5521 cgcatcttcg agatcagccg ctttgccagc cttggcctgc caatggaggc gccgggcatg
     5581 tggatctcag ggctgggcct gctgatcgtc gtgtggggcg tcgccgacct tctcactgag
     5641 cggcctacaa cctactgact gtcctccccg aggaattccc ggacgagcga ctggaagtgg
     5701 tagacgccgt tctcgcgctt ggcgctgaag cggcccgtgt cgtaggcgcg cgagcgcagg
     5761 ccgcgctgga cctgctcgca cagcacgatg tcctcctgct ggatctggtc agaaaaggcg
     5821 atgccctgct gcatcgactc ccagcccgcg cctgaaccag gctccgcgaa gaaccactcg
     5881 aagatcgtca gcgtccggtc gtggcccacc ggcaagatga cgttggacga catgttgtcg
     5941 gggtagatgt tgaacatcgt gttcggaaag agccagtagt agagggcgtc ctcctcgccg
     6001 tccgcggtgc gcacgaagcg tcggtcgcgg ccgatctcct ggccttcctt cagctcacgg
     6061 ataggggcgt gctgcttgga gtagtagcgc tgcgtctcga cccggtaggc gtcgtagtcg
     6121 agctcgcgga acaggtcggg gtgggcgatc ggcaggtgat agccctcgag gtagttgtcg
     6181 acgtagacct tccagttgca ctcgatcagg tattcgcgtc gttcgacgag gcgcatgtga
     6241 tcgacctcaa agccggcacg cgccacctcg tccgggatag cgcccaggac ctcgctgagc
     6301 ggcggcgccg cgtcgtccag gttcgcgaac acgaatggcc cccaacgctc gacgcggacc
     6361 ggcgtaaggc cgaagtcctc cttgcggaag tcctccgtct cttccatctc cgggcaggcg
     6421 cgcagcgtcc catcgaggcc gtacgtccag ccgtggtagc ggcactgcag tgaccggcgg
     6481 ttgccctttg agagcgcgac ctggccggcg cggtggcggc acacgttgta gaagccgcgc
     6541 agcacaccgt cggtaccgtg ggtgatgacg atgggctcat cgaggatcgt ggtgggcacg
     6601 aagtcgcccg gccgagccag gtcctcgtgg cgcgccacga gctgccacgt gcgaccgaac
     6661 acctgctcct tctcgcgttc caggtagccc ggctcgaggt agtagcgcgc cggcagagtt
     6721 gatgcctggg ccagcggaat cgcacgactt tggtctggca ccgccccact catggccgta
     6781 gagtgtacgg acgcatccag gcgagggagt ctgcgcgttc gagcaacgac ttttgaggag
     6841 gccactgatg attccccggc gagccgcatt catagttgcg gcaactctca tcttcgcggc
     6901 ggcctgtgcg ggcccggcag cgtcccccac accggtaccc acacccacat cggcacctag
     6961 cgccgcgtcc accgcggtat gcgacaacct ccccgcggga tcggaaggcg atctgctggc
     7021 cacgatttgc gccgccggca agatcgtgat ttccacggac gcggactacg cgccacagtc
     7081 ctcggttacc ccgagcggtg gctacgaggg cttcgatatc gacgttggcg aggaaatcgc
     7141 cagccgtctc ggtgtcgatg cggagtggag cgcgcagatg tgggacacgg tcatcgctgg
     7201 cagctggtcc ggccggtggg acatgagcgt cggctcgatc accgtgtgct gcgatcgcga
     7261 gcagctcttc gactttacgc agccgtacta cttcacgccc gcccaggttg cagtcctcga
     7321 gggctcaggc ttcgcatcca tcgacgacat cgcgggcgag acggtttgcg tcggcgaaga
     7381 cacaacctat ctctattggg tcgagggtac gctcgagctg gctgcgccca acaggatt
//
LOCUS       DATBRX010000022         7066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1235268, whole genome shotgun sequence.
ACCESSION   DATBRX010000022 DATBRX010000000
VERSION     DATBRX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235268"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1081)
                     /locus_tag="VIF08_00785"
     CDS             complement(167..1081)
                     /locus_tag="VIF08_00785"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8134547.1"
                     /translation="MSLAEVAPGFVRSGKVRDIYALGDARLLLVASDRISAFDVVLPT
                     PIPDKGRVLTGLARYWFRETERAGIMSNHLLSTSVEGLPGEFAAAADELRGRSMVCRR
                     ATVLPVELIVRGYLAGSGWKDYGKTGAVCGIPLPAGLRESDRLPEPIFTPSTKAEVGH
                     DENISFDQMVALVGPARAEEARAKALALYNFAAQTAEPAGIIVADTKFEFGIVDDGPM
                     ILIDELLTPDSSRFWDASTYEPGRAQDSFDKQYVRDYLETLDWGKTYPGPELPAEVVA
                     GTRRRYAEAFERITGGSFERYLADDVIA"
     gene            complement(1068..2384)
                     /gene="purB"
                     /locus_tag="VIF08_00790"
     CDS             complement(1068..2384)
                     /gene="purB"
                     /locus_tag="VIF08_00790"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8134548.1"
                     /translation="MIARYTLPEMGALWTDQARFEHMLRVELAALRAVERHGLVPAGT
                     TQAVAATARVDVERIAELEQATDHDVIAFVSQVAESCGPEGRYLHFGLTSSDVVDTAL
                     ALQCRAAAELIDAALAGLIDAVTAKAREHAATVMMGRTHSVHAEPITFGLKLASWAFE
                     LARGRRRIAAAAEDIAVGKLSGAVGTYSQLGPEIEAEVMAELGLGMEPAATQVVARDR
                     HAAFLAAIAVCAGSIERAATEVRNLQHTEIAELQEPFRAGQKGSSAMPHKRNPITSER
                     LAGLARLIRGYAGAGLEDEALWHERDIAHSSVERVALPGATTLLHYMCVRMTRIVEGL
                     IVRPERMRENLERGLGLFASSRLLTALVEQGGLAREDAYAIVQRAALKAADERRQLRE
                     LIEADSDVTGRLSADQVAACFDIDHLLRNVETVIDRLKGEAAHVAG"
     gene            complement(2381..3694)
                     /gene="purD"
                     /locus_tag="VIF08_00795"
     CDS             complement(2381..3694)
                     /gene="purD"
                     /locus_tag="VIF08_00795"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY8134549.1"
                     /translation="MSGLTVLVVGAGARQHALCWRLAAEAGVGHIIVAPGNPLIHDVA
                     EMRADVDGEDVGAIVALAVAEHVDLVVVGPEDPLVGGLADRLADVGIACFGPSAAAAR
                     LEGSKAFAREVCRAAGVSMARGRAFTDSRSAIEFAATLDAPLVVKADGLAGGKGVTIA
                     ASLGEAERAIRESLESGRFGGAGAMVVVEQYLSGREASVFALCDGTSYALLPAARDHK
                     RAFDLDGGPNTGGMGAFSPVPELDDAALSAIGEMIVAPVLREMEGRGAPFRGVLFCGL
                     MLTSDGPRVLEFNIRFGDPEAQAILPRVDVPLAQLMLECATGRLTASGVLPVHPLATV
                     ALVLAADGYPETARRGDVIRGIEAARAGGALVFGAGVARDDDGELVTAGGRVLTVVGT
                     GADVAAAADAAYAAADLIDYAAKWSRRDIGRALEASAVAGPGVAF"
     gene            complement(3735..5036)
                     /locus_tag="VIF08_00800"
     CDS             complement(3735..5036)
                     /locus_tag="VIF08_00800"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY8134550.1"
                     /translation="MPATVICGLQWGDEGKGKATDLLAGAVSVVVRYQGGDNAGHTVV
                     VGDEVFKLHLIPSGVLHAHITPIIGPGVVVNPRTLLSEIQMLNERGIATDKLRVSSAA
                     HVIMPYHVALDTAREGAAADGGIGTTKRGIGPAYGDRAARVGVRMGDLLEPELLRERL
                     AAAVGEKNALLVGAYGAPPVELEALYAEAVGWGEQLAVKITDTTALVQRALADGEHML
                     LEGAQGTMLDLDHGTYPYVTSSNPVAGGACTGSGVGPLQIDEVLGILKAYSTRVGAGP
                     LPTELKDAVGDHLTEVGREYGTTTGRRRRSGWLDAMPLRYAVQVNSASSLMLNKLDIL
                     TGLAQLKMCVAYRVDGRLVDSWPLPLAQLERAEPVYETFDGWTENLGGVRSIAELPKA
                     TRRYMAAIEERAGAPISVVSLGPERNQTIRVDGPRPVRSAA"
     gene            complement(5307..6173)
                     /locus_tag="VIF08_00805"
     CDS             complement(5307..6173)
                     /locus_tag="VIF08_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HEY8134551.1"
                     /translation="MALQPGPSSGPIDPQVYPSRNARSVKMSRMRKRAVALLGLIAAQ
                     GLLLPGPADAARWAPSPGTAWQIQFSGRIDRSVGADAFDLDLFDTPATTVAALHAKDR
                     RVVCYINAGAWEEWRPDAGRFPGSVKGRELDGWPGERWLDIRRIDILGPILTDRLDMC
                     RSKGFDGVEFDNVDGYSNRSGFPLTAADQLTFDKWLAARAHERGLAVGLKNTLDLAAE
                     LEPHFDFAILEQCFQYRECGLVAPFIESRKSVVDIEYKLSRGQFCARAADLGIVAMRK
                     RLNLDAWRRACP"
     gene            6309..>7066
                     /locus_tag="VIF08_00810"
     CDS             6309..>7066
                     /locus_tag="VIF08_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY8134552.1"
                     /translation="MLVLTMSVMIVSMLPGPVQAGWLTDVWPGSTASPDSEAGQLLEL
                     ARRQVGKPYRYAAIGPDAFDCSGLVWFLFKTTGLADRMGGKRRGAAGYLKWFRENMPN
                     QVSHNLADARPGDILIWGNGKHAGIYISGNWAVSALNSRYDVRIHRADPMGLPFTAVL
                     KVSMSRGGGGGGGEPTPTPDPDATPAPTTDPGGLPMPPTDVDAVPGDDLTVEVSWDGA
                     TGDATPLKYRVYRNGAYYIGIRYDTFVDHPWLPG"
BASE COUNT         1056 a         2609 c         2331 g         1070 t
ORIGIN      
        1 ccttcggccg agtcggcctc caccgtcagc tccactcgcc ttccgacccg cacggcatgc
       61 acgttctccg cgtgaagctg gggcagcgcg gcttccacgg cgcgtccggc cgggtcgagg
      121 atgccgtcgc ggggcatgat cgagacgctg aagcggtaag cggcgctcat gcgatcacgt
      181 catctgcaag gtagcgctcg aacgagccgc cggtgatgcg ctcgaacgcc tcggcgtagc
      241 gccgccgcgt cccggccacg acttctgccg gcagctctgg tcccgggtag gtcttgcccc
      301 agtccagcgt ctcgaggtag tcgcgcacgt actgcttgtc gaacgagtcc tgcgcgcggc
      361 cgggctcgta ggtggatgca tcccagaacc gcgacgaatc gggcgtgagc agctcgtcga
      421 tgaggatcat cgggccgtcg tcgacgatgc cgaactcgaa cttggtgtcg gcgacgatga
      481 tgcccgccgg ctcagcggtc tgcgcagcga aattgtagag agcgagcgcc ttggcgcgcg
      541 cctcctctgc ccgcgccggg ccgaccagcg cgaccatctg gtcgaacgag atgttctcgt
      601 cgtggccgac ctccgccttg gtcgacggcg tgaagattgg ctcgggcagg cgatcggact
      661 cacgcaggcc ggcgggcagc gggatgccgc ataccgcgcc ggtcttgccg tagtccttcc
      721 agccgctccc cgccaggtag ccgcgcacga tcagctccac cggcaagacg gtcgcgcggc
      781 ggcagaccat ggagcggccg cgcaattcgt ccgcggcggc ggcgaactcg ccgggcaggc
      841 cctcgactga ggtcgagagc aggtggttgg acatgatccc cgcgcgctcg gtctcgcgga
      901 accagtaccg cgcgaggccg gtcaggacgc ggcccttgtc gggaatgggc gttggcaaca
      961 cgacgtcgaa cgcgctgatc cggtccgagg ccaccaatag caggcgcgca tcgccgagcg
     1021 catagatgtc gcgcaccttg cccgatcgaa cgaagcccgg tgcgacctca gccagcgaca
     1081 tgagcagcct cccctttcag tcggtcgatg accgtttcca cattgcgcag caggtgatct
     1141 atgtcaaaac aggctgcgac ctggtcggcc gacaacctgc ccgtcacgtc ggagtccgcc
     1201 tcgatgagct cgcgcagctg gcgccgctcg tccgcggcct tcagcgcggc ccgctggacg
     1261 atcgcgtagg cgtcctcgcg cgcgaggccg ccctgctcga cgagcgcggt cagcagccgg
     1321 ctgctcgcga agagacccag gccgcgctcg aggttctcgc gcatccgctc tggccgcacg
     1381 atgaggcctt cgacgatgcg cgtcatgcgc acgcacatgt agtgcagcag cgttgtcgcc
     1441 ccgggcagtg cgacgcgctc cacggatgaa tgggcgatgt cgcgctcgtg ccacagcgcc
     1501 tcgtcctcga gacccgcccc ggcgtagccg cggattaggc gcgccaaacc ggcaagccgc
     1561 tccgaggtga tggggttgcg cttgtggggc atcgcgctcg agcccttttg ccctgcgcgg
     1621 aacggctcct gcagctcggc gatctccgtg tgctggaggt tgcgcacctc tgttgcggca
     1681 cgctcgatcg agccggcgca gacggcgatg gcagcgagga acgcggcgtg gcgatcgcgc
     1741 gcgaccacct gggtcgcggc aggctccatg cccaacccga gctcagccat gacctcggcc
     1801 tcgatctccg gcccgagctg gctgtacgtc ccgaccgcgc cgctcagctt gcccacggcg
     1861 atgtcttcgg ccgcggccgc gatccggcgc cggccgcggg ccagctcgaa cgcccacgac
     1921 gcgagcttca ggccgaacgt gatcggctcg gcatggaccg agtgagtgcg gcccatcatc
     1981 accgtggccg cgtgctcgcg cgccttggcc gtgactgcgt cgatcaggcc cgcgagcgca
     2041 gcgtcgatca gttctgcggc tgcgcggcac tgcagcgcga gcgcggtgtc gaccacgtcg
     2101 gatgacgtca ggccaaagtg caggtatcga ccctccggtc cgcagctttc cgcaacctgg
     2161 ctgacgaagg cgatgacgtc gtggtcggtg gcttgctcga gctcggcaat gcgctcaacg
     2221 tcgacccttg ccgtcgccgc gaccgcctgc gtcgttccgg ccggcaccag gccgtggcgc
     2281 tctactgcgc gcagcgcggc gagctcgacg cgcagcatgt gctcgaagcg cgcctggtcc
     2341 gtccacagcg cgcccatctc cggcagcgtg tagcgcgcga tcaaaatgcc actcccggcc
     2401 cggccaccgc cgacgcttcg agcgctcggc cgatgtcgcg gcgcgaccac ttggcggcgt
     2461 aatcgatcag gtcagccgcg gcgtacgcag catcggccgc cgcggcgacg tccgcacccg
     2521 tgccgacgac cgtcaggacg cggccgccgg cggtcacgag ctcgccatca tcgtcccgcg
     2581 ccaccccggc gccgaatacc agcgccccgc cagcccgcgc ggcctcgatc ccgcggatca
     2641 cgtcgccgcg acgcgccgtt tccgggtacc cgtcggcggc cagcactagc gcgaccgtgg
     2701 ccagcgggtg caccggaagc acgcccgaag ccgtgagtcg gccggtcgcg cattcgagca
     2761 tgagctgggc cagcgggaca tcgacgcgcg gcaggatcgc ctgcgcctcc ggatcgccga
     2821 agcggatgtt gaactcgagc acgcgcgggc cgtccgatgt cagcatcagg ccgcagaaga
     2881 gcacgccacg gaacggcgcg ccacgcccct ccatctcgcg caggaccggc gccacgatca
     2941 tctcgccaat ggcgctcagg gccgcgtcgt cgagctcggg aacgggcgag aacgcgccca
     3001 taccgccggt gttcggcccc ccgtccaggt cgaaggcacg cttgtggtcg cgcgccgccg
     3061 gcagcagcgc gtacgacgtg ccatcgcaca gcgcaaagac gctcgcctcg cgtccgctca
     3121 ggtactgctc aacgacgacc atcgcgcctg cgccgccaaa ccggccgctc tcgagcgact
     3181 cgcggatcgc gcgctcggcc tcgcccaggc tggcggcgat cgtgacgccc ttgccccccg
     3241 ccaggccgtc ggccttgacg acgagcggcg cgtcgagggt cgccgcgaac tcgatcgccg
     3301 agcggctgtc agtgaaagcg cggccgcgcg ccatgctcac gcccgccgcg cggcacacct
     3361 cgcgggcgaa agccttgctg ccttcgagcc gcgccgccgc ggcgcttggc ccaaagcagg
     3421 cgatgccaac gtcggccagc cggtcagcga gtccgccgac cagcggatcc tctggcccca
     3481 cgacgaccag gtcgacgtgc tccgcgacgg ccagcgcgac tatcgcgcca acgtcctcgc
     3541 catcgacgtc cgcccgcatt tccgcgacat cgtggatcag cgggttgccc ggcgcgacga
     3601 tgatgtgacc cacgccagct tcggcggcca accgccagca cagcgcgtgc tgccgcgccc
     3661 ccgcgccgac taccaggacg gtcagcccgc tcaagccact cccctcacgc cactccccct
     3721 cacgccactg cccctcacgc cgcgctccgc accggccgcg gcccgtccac gcggatcgtc
     3781 tggttgcgct cgggtcccag gctgaccact gaaatcggcg cgccggcgcg ctcctcgatg
     3841 gccgccatgt agcgccgcgt ggccttcggc agctcggcga tcgagcgcac gccgcccagg
     3901 ttctcggtcc agccgtcgaa cgtctcgtat acgggctccg cgcgctccag ctgcgcgagg
     3961 ggcagcggcc acgaatcgac gagccgcccg tcaacccggt acgcgacgca catcttgagc
     4021 tgcgccaggc cggtcaggat gtcgagcttg ttcaacatga gcgagctcgc gctgttgacc
     4081 tgcacggcgt aacgcaacgg catcgcatcg agccagccag agcgccgtcg ccggcccgtg
     4141 gtcgtgccgt attcgcgtcc gacctcggtc agatggtcgc ccacggcatc ctttagctcg
     4201 gtcggcagtg gccccgcgcc aacgcgcgtc gagtacgcct tcaggatccc caggacctcg
     4261 tcgatctgca gcggcccgac accgctgccc gtgcaggcgc cccccgcgac cgggttggac
     4321 gaggtcacgt acgggtaggt gccatggtcc aggtcgagca tcgtcccctg cgcgccctcg
     4381 agcagcatgt gctcgccatc cgccagggcg cgctggacca gggccgtcgt gtcggtgatc
     4441 ttgacggcca gctgctcgcc ccagccaact gcctcggcat acagtgcctc gagttcgacc
     4501 ggcggagcac cgtaggcacc gaccagcagc gcgttcttct cgcccaccgc cgcggccagc
     4561 cgctcgcgca gcagctcggg ctcgagcagg tcgcccatgc gcactcccac gcgcgccgcc
     4621 cgatccccat atgccggccc aatgccgcgc ttggtcgtgc cgatgccgcc atccgcggcg
     4681 gcgccctctc gggccgtatc cagggccacg tggtagggca tgatcacgtg cgccgcgctg
     4741 ctcacccgca gcttgtcggt ggcgatgccg cgctcgttga gcatctgtat ctcgctgagc
     4801 agcgtccgtg ggttgacgac cacgcccggg ccgatgatcg gcgtgatgtg cgcgtgcagc
     4861 acaccgctcg ggattaggtg gagcttgaag acctcgtcgc cgacgacgac tgtgtggccg
     4921 gcgttgtcgc cgccctggta gcgcacgacg accgagacgg ccccagccag caggtcggtc
     4981 gccttgccct tgccctcgtc gccccattgc aggccgcaga tgaccgtcgc aggcatggct
     5041 agcgccccgc cctgaaattg ctgggaacga ggagcgagca agttagtccg gtgccctgac
     5101 cgactccgcc gtgcgtgagt cgcatgtggc tgcgtgccgg cctcacggga ccgacgccgc
     5161 cgtcgggcaa caactcctcc gccgctttct gtgggcgaga actcacgggc tcccgcgccg
     5221 ccgaagggcg cagtctagca gcgaccccga cttgggacct cgtctaccgg cataccccta
     5281 accggcacac ccttacaggc acgcttctat gggcacgccc tgcgccacgc gtccaggttg
     5341 agccgcttgc gcatggcgac gatccccagg tccgcagctc tggcgcagaa ctggccgcgg
     5401 ctgagcttgt actcgatatc gaccacgctc ttgcggctct cgatgaacgg cgcgacgagc
     5461 ccgcactcgc ggtactggaa gcactgctcc aggatcgcga agtcgaagtg cggctcgagc
     5521 tcggccgcca ggtcgagcgt gttcttcagg cccaccgcca ggccgcgctc gtgcgcgcgc
     5581 gccgcgagcc acttgtcgaa agtgagctga tcggccgcgg tcagcgggaa gccactccgg
     5641 ttcgagtagc catccacgtt gtcgaactcg acgccgtcga agcccttgga gcggcacata
     5701 tcgagccggt cggtcaggat gggcccgagg atgtcgatgc gccggatgtc gagccagcgc
     5761 tcgcccggcc agccatcgag ctcgcgcccc ttgacggagc ctgggaagcg ccctgcgtct
     5821 gggcgccact cctcccacgc gccggcgttg atgtagcaga cgacgcgcct gtccttcgcg
     5881 tgcagcgccg ccaccgtcgt ggctggcgtg tcgaacaggt cgagatcgaa cgcatcagcg
     5941 cccacggagc ggtcgatccg gccgctgaac tggatctgcc aggccgtccc cgggctgggc
     6001 gcccagcgcg ccgcgtccgc gggacccggc aggaggaggc cctgcgccgc gatcagcccc
     6061 agcagcgcga ccgcccgttt ccgcatgcga ctcatcttga ccgacctggc gttgcgcgat
     6121 gggtagacct gggggtcaat tggcccggac gatggtccgg gttgcagtgc catgtcgcgc
     6181 gcctagtgtc gtttcgtcct tatccgcgtg ccccagcact tatagacttt ccacgggagg
     6241 gggaagccgt gaacttcgtt ccagccgcta ctcgagcgac cgcacatgcc ggccttcgcg
     6301 gccggctgat gttggtcctc accatgtcgg tcatgatcgt cagcatgctg cctggcccgg
     6361 tccaggcggg ctggctgacc gacgtttggc caggatcaac ggcgtcgccc gattccgaag
     6421 ccggccagct gttggagctg gcgcgccggc aggtgggcaa gccctaccgg tacgccgcca
     6481 tcggccccga tgccttcgac tgctcgggcc tggtgtggtt cctgttcaag acaaccgggc
     6541 tcgccgatcg catgggcggc aagcgccgtg gcgcagcggg ctatctcaaa tggttcaggg
     6601 agaacatgcc caaccaggtt tcgcacaacc tcgccgacgc ccgcccaggc gacatcctga
     6661 tctggggcaa cggcaagcac gccggcattt acatcagcgg caactgggcc gtcagcgcgc
     6721 tcaacagtcg ctatgacgtg cgcatccacc gtgccgaccc catgggcctg cccttcaccg
     6781 cggtcttgaa ggtcagcatg tcacgcggcg gtggcggtgg cggtggcgaa cccaccccga
     6841 ctcccgatcc cgacgccacg ccggctccca ccaccgatcc gggggggttg ccgatgccgc
     6901 cgaccgacgt cgacgccgtg cccggcgacg acctcacggt cgaagtcagc tgggacggtg
     6961 ccacgggcga cgcgacgccg ctcaagtacc gcgtctatcg aaatggcgcg tactacatcg
     7021 gcatcaggta cgacaccttt gtcgatcacc cctggctgcc cggcca
//
LOCUS       DATBRX010000023         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1570596, whole genome shotgun sequence.
ACCESSION   DATBRX010000023 DATBRX010000000
VERSION     DATBRX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570596"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3383)
                     /locus_tag="VIF08_00815"
     CDS             complement(<1..3383)
                     /locus_tag="VIF08_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8134553.1"
                     /translation="MICANCGTNNEAGRRFCDECGALLAAACPNCGATDNRPTAKFCG
                     TCGATLNAAAVPTSGAGAGATSSSATAAERRFVSVLFVDLVGFTPFAEERDAEQVRDT
                     LDRYFGLARDAVERHGGVIEKFIGDAVMAVWGTPTAHEDDAERAVRAAFELVDAARGL
                     GDGVQARAGIVTGEAAVTIGAEGQGMVAGDMVNTASRLQSVAPAGAVLVSETTMNSAK
                     AAIVFESVGAQELKGKTAPVAAYRALRVVANRGGGQRSEGLEAPFSGRDEELRLLKEL
                     LHATTRDGRARIAAISGPAGIGKSRLTWEFEKYIDGVVETIYWHRGRSPAYGEGVTFW
                     ALGEMIRRRAELAENDDEATSRARIATTVAQYVTDPDERAWVEPALLTLLGLEPPPPG
                     GRDALFAAWRIFFERIADQATTVLVFEDIQWADSGLLDFIEHLLEWSKSKPLFVVALT
                     RPELYERKPGWGTGHRLATQMPLEPLPEAAMRQLLGGLAPGLPEQAVTAILSRADGIP
                     LYAVETIRMLVADGRLTLTDGAYTPTGDLGELAVPETLRSLIASRLDALDPTDRTLLQ
                     DGAVLGQRFTTAALAATSGMTAEEIEPRLRALVRREFLELEADPRSPERGQYGFVQSL
                     IREVAYGTLARRDRRSRHLAAARYFEMLSDDELAGVLASHYLAAHEASEAGPEADALA
                     VQARLALRGAAERAAALGGHAQALVYLEQALAVSTDPGERATLLHLASESANASGHDA
                     KAQDYARQVIDYHASVGDEASRMRAVGLLGRVLLDNGHLDEAVEFMEGELSGEVSDSP
                     DSAQVLANLSRAYMRTDKNQDSVDAADRALKIADRLNMERVVAEAFINKASSLHRLGR
                     IREAVALHQAALAIADRHGFAEQQLRGRNNYSVSQIELEPTKALQTIHEAIDLARRLG
                     QRGMYNWLIGTGAMYSASIGTGWERALELEQEVLASSPIPYDRARALLTSSLLHARRG
                     DPIDMALLREATAGITDGQVVGGFDFVQAEVALTENRWADAHSLSFRALTEWVDSTGL
                     VMQPALHAAAVTKRAGDAQEIKARMDAHASGTPTADAMRAWTDGVVDALTGSAAEALR
                     EIRMAYEATISLGFAFDAATIVIDALRLFPDEAEVHAWVP"
BASE COUNT          500 a         1206 c         1185 g          549 t
ORIGIN      
        1 ggcacccaag cgtgaacctc cgcctcgtcc gggaagagac gcaatgcgtc gatcacgatg
       61 gtcgccgcgt caaatgcgaa gcccagggaa atcgtcgcct catacgccat tcgaatctcg
      121 cgcaacgcct ccgcggcgct accggtcagg gcgtctacga ctccatctgt ccacgcgcgc
      181 atcgcgtccg cggtcggcgt gccgctggcg tgggcatcca tgcgcgcctt gatctcctgc
      241 gcatcgcccg cccgcttcgt tacggcggcg gcatgcagcg cgggctgcat gactaatccg
      301 gtggagtcga cccattcagt gagcgcccgg aatgaaagtg agtgcgcgtc cgcccatctg
      361 ttctccgtca gcgcgacctc tgcctgcacg aagtcaaacc cgccaactac ctgaccgtcg
      421 gttattcccg cggtcgcctc cctgagcagc gccatgtcga tcgggtcgcc acgccgggca
      481 tgcagcagcg agctggtcaa caaggcccgg gcgcggtcgt aggggatggg cgagctcgcc
      541 aggacctcct gctcgagctc cagggcacgc tcccagccgg tgccgatgga cgccgaatac
      601 atcgcgcccg tgccgatgag ccagttgtac atgccgcgct ggccgagccg gcgcgcgagg
      661 tcgatggctt catggattgt ctggagcgcc ttggtaggct cgagctcgat ctgagagacg
      721 ctgtagttgt tgcggccacg cagctgctgc tcggcgaagc catggcggtc cgcgatggcg
      781 agggcggcct ggtgcagcgc cactgcctcg cgaatgcggc ccaggcggtg gagcgacgag
      841 gccttgttga tgaatgcctc ggcaacgacg cgttccatgt tcagccggtc ggcaatcttg
      901 agggcgcggt cggccgcatc gaccgaatcc tggttcttgt ccgtgcgcat gtaggcccgc
      961 gacaggttgg ccagaacctg cgccgaatcg gggctgtcgc tgacttcgcc cgacagctcg
     1021 ccctccatga actccactgc ttcgtccagg tggccgttgt cgagcagcac ccgaccgagc
     1081 aggccgactg ctcgcatgcg cgacgcctcg tcgcccacgc tcgcgtggta gtcgatcacc
     1141 tggcgcgcgt agtcctgggc cttggcatca tgccccgagg cgttggccga ctcagacgca
     1201 agatgaagca gcgtcgcgcg ctcgcccgga tccgtgctga ccgccagcgc ctgttccagg
     1261 tatacgagcg cctgggcgtg gccgcccagt gcagcggcgc gttcggccgc tccccgcagg
     1321 gcgaggcgcg cctggacggc gagcgcgtcg gcctccggtc cggcctcgga cgcctcgtgc
     1381 gcggccaggt agtggctggc caggaccccg gcgagctcgt cgtcagagag catctcgaag
     1441 tagcgcgcgg ccgcgaggtg gcgcgagcgc cggtcgcgcc gcgccagcgt cccgtacgcg
     1501 acctcacgaa tcagcgactg gacgaagccg tactggccgc gctccggtga gcgcggatcc
     1561 gcctccagct ccaggaactc gcgccggacg agcgcgcgca gccgcggctc gatctcttcg
     1621 gccgtcatgc ccgaggtcgc ggcaagcgcc gcagtggtga agcgctggcc cagcaccgcg
     1681 ccgtcctgga gaagggtcct gtcggtggga tcgagcgcgt cgagacgcga ggcgatcagt
     1741 gagcgcagcg tctccggcac ggcgagctcg cccaggtcgc cggtcggcgt atatgcgccg
     1801 tcggtcagcg tgagacgccc gtcggcgacg agcatgcgga tcgtctcaac cgcatagagc
     1861 gggatgccat cggcgcggct gaggattgcc gtcacagcct gctcaggcag gcccggcgcg
     1921 agcccgccga gcagctggcg catcgcggct tccggcagcg gttcgagcgg catctgtgtc
     1981 gcgaggcggt ggcccgtccc ccagccgggt ttgcgctcgt acagctcggg tcgggtgagc
     2041 gcgacgacaa agagcggctt gctcttggac cactcgagca ggtgctcgat gaagtcgagc
     2101 aggcccgagt cggcccattg gatgtcctcg aagaccagga cggtcgtcgc ctggtcggca
     2161 atgcgctcga agaagatgcg ccacgcggcg aagagcgcgt cgcgcccgcc gggcggtggc
     2221 ggctcgagac ccaggagggt gaggagcgcc ggctcgaccc aggcccgctc gtcgggatcg
     2281 gtgacgtatt gcgcgaccgt cgtggcgatg cgcgcgcggg aggtggcctc gtcgtcgttc
     2341 tcggcgagct cggcgcggcg gcggatcatc tcgcccagtg cccagaaggt cacgccctcg
     2401 ccgtacgccg gggagcggcc gcggtgccag tagatcgtct cgacgacgcc gtcgatgtac
     2461 ttctcaaact cccaggtcaa gcggctcttg ccgatgcctg ccggaccgct gatggccgcg
     2521 attcgcgcac gcccgtcgcg cgtggtcgcg tggagcagct ccttgagcag gcgcagctct
     2581 tcgtcgcggc cgctgaacgg cgcctctaac ccctcgctgc gctggccgcc accgcggttg
     2641 gcgacgaccc tgagcgcgcg gtaggcggcc accggcgccg tcttgccctt cagctcctgc
     2701 gcgccgaccg actcgaagac gatcgccgcc ttggccgagt tcatcgtcgt ctcgctgacg
     2761 agcaccgctc ccgccggagc caccgactgc aaccggctgg ctgtgttcac catgtcgccg
     2821 gcgaccatgc cctggccctc cgcgccgatc gtcaccgcgg cctcgccggt cacgatcccg
     2881 gcgcgcgcct ggacgccgtc acccaggccc ctggccgcgt caaccagctc gaacgcggca
     2941 cgaacggcac gttcggcgtc atcttcgtgc gcggtcggcg ttccccacac ggccatgacc
     3001 gcgtcgccga tgaacttctc gatgacgccg ccgtggcgct ccaccgcgtc acgagccagg
     3061 ccgaaatacc tgtcgagggt gtcgcggacc tgttcagcgt cacgttcctc tgcaaatggc
     3121 gtgaagccga ccagatctac gaagagcacg ctcacgaagc gtcgctcggc cgcggtcgcg
     3181 ctggagctcg tcgcgccggc gccggcgccg gaggtgggga cggccgcggc gttgagcgtc
     3241 gctccgcagg tcccgcagaa cttggccgtt gggcggttgt cggttgcccc gcagttgggg
     3301 caagcagccg cgagcagcgc gccgcattcg tcgcagaagc gtcggccggc ttcgttgttg
     3361 gtgccgcagt tagcgcagat catgtgcgcc ctctcatggg ctcgatgata accacgcctg
     3421 atcaggccta cgatcagcgt
//
LOCUS       DATBRX010000024        13879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_118451, whole genome shotgun sequence.
ACCESSION   DATBRX010000024 DATBRX010000000
VERSION     DATBRX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13879
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118451"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..397)
                     /locus_tag="VIF08_00820"
     CDS             complement(17..397)
                     /locus_tag="VIF08_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134554.1"
                     /translation="MTDRATADATEGGSRDHPGPAMPEYKGAPLDAERGPGLGCFWIQ
                     IALLVALLILTPLSVLWAWPSEVSAALLILSLVLLFFTGQTMIFLLRLVAADRRSRRR
                     PNSPSARRTVGQLEDESDKDHAAP"
     gene            436..975
                     /locus_tag="VIF08_00825"
     CDS             436..975
                     /locus_tag="VIF08_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8134555.1"
                     /translation="MFEIREAKASDARGIAEVRVHTWQEAYRDIMPTAFLNGLSVDAD
                     EKRWRDLLKAQAPNRRMLVADSGGQVVGFVTAGVLREENAQPRTGEVYAIYVLPDWWG
                     QSIGRGLLAHAEQALIEQGYDAAVLWVLADNRRARAFYERAGWHPDGGTKSATFGGRE
                     VEEVRYRIALGRSRVDEPA"
     gene            complement(930..1919)
                     /locus_tag="VIF08_00830"
     CDS             complement(930..1919)
                     /locus_tag="VIF08_00830"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8134556.1"
                     /translation="MTADPRHLLDVDEWSDETIEAVLDNASRARDAGERPGNELLGCR
                     VVLLFAEPSTRTRMSFEMAARGLSAETYVVDPASSSMVKGETLVDTVRNLDALGVDMI
                     VMRHHRAGAPWVVARHFGGAVVNAGDGWHAHPTQALLDLLTLRRAFGGSRDALRGRKI
                     AIVGDLLHSRVARSNVWTLTRAGADVWVSGPDSWLRGYSELPVTTKTDLEAALDGASA
                     VMGLRVQKERMRGSALSIGDYIARYQITEERLVRFAPEARYLHPGPINEGVEVTRAVA
                     RGPRSLVLEQVANGLAVRMAVLALLAPSTPRGLRGPTRAQAGSSTRDLPSAIR"
     gene            complement(1916..2476)
                     /gene="pyrR"
                     /locus_tag="VIF08_00835"
     CDS             complement(1916..2476)
                     /gene="pyrR"
                     /locus_tag="VIF08_00835"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY8134557.1"
                     /translation="MTSRQIMTTDDVRRALVRISHEIVEKHGGTDGLALVGIQRRGVP
                     IAERLAASIKEHEGVQPPVGSLDITFYRDDLSQVAKAPLLKATDLPFALEASTIVIVD
                     DVLYTGRTVRAALDALVDVGRPKAVRLAVLVDRGHRELPIRADHVGKNVPTSRDEVIK
                     VHLREVDGGDDDVVIERRAPGAEDGA"
     gene            complement(2525..4420)
                     /locus_tag="VIF08_00840"
     CDS             complement(2525..4420)
                     /locus_tag="VIF08_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY8134558.1"
                     /translation="MKMARARADHASWRELIAARLDRPLTRSETRSLAAHLRACAACR
                     EADRDYRQQRALLRALPLRPVPRNLWPRTSAALDREVARGSSRFGRTWRRRGRKFGPS
                     AALMATIASLGVVVGLTAMQLTPAVSIASPSVPAPATPFAIAPQQLAFVGSDDAHMYV
                     YRTDVGHACPANAAGCAVDEGIVRTPVNLPASVRARNLALSPSGRTLALVGSGGGHDV
                     IAVVLLSDGAHDPTPPDGTPAPVNPGATPVTVTGPGQPEATPSDAPPVVVAILENVES
                     AGAPPAWSPSGEMLAFSAMPADGSHGPDVYVWSPSDAVARPVTTDHGSYFASWSTGRI
                     VISRLAGVGNGEGSDGASLSTIVMDPSTSEERPVAGPALWLPVVNPAGTRAIAWRGDL
                     DFASGHPVPSSGALLMIDWAAIDPFAAANEPVATPDVVETPTPTDAPAATDAPAPTDA
                     PASTHPSDPPTAPPASPSASPVVASTEPAPSEGPSATPEPQSTETPVEPSETATPSAL
                     PDGWVELDLGRDTATTPVLDWQARWSLDGQVLGIWLADTVGSTWGRLAVMAVDPETGA
                     VASSEALLSATLARRGFSLGLSRVVWVAPSDDNPDGELRIRTWGDDGVGGVRLEPLTL
                     DGVVPAF"
     gene            complement(4395..5003)
                     /locus_tag="VIF08_00845"
     CDS             complement(4395..5003)
                     /locus_tag="VIF08_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8134559.1"
                     /translation="MSDDRASARAFVERLFAAHHAEIYAYLCRMVRDDELAADLTQEA
                     FVKAYRAYDTLEDPNRARAWLYQIAGRTALDELRRRKIVRFVPWTGESRGTAASAEEL
                     VMRARLSGDLERALARIPARQRQALLLSELNDMTGLELADALGVSHVAARALLTRGRE
                     SLRQALAIERANSAVEDEKRARAAAPVEARHRRDDEDGPRSR"
     gene            complement(5130..6170)
                     /locus_tag="VIF08_00850"
     CDS             complement(5130..6170)
                     /locus_tag="VIF08_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134560.1"
                     /translation="MPRGLFELRKDAITGWWVAVVVDREFERQRFFRPARHIGQTTDD
                     CPNCDLAAGGEQVQIRTLKQDAFIVAGTEKEGREAQPAGREPELGMVGDSGSYQTIVA
                     PRGHHESLAETSPQIAYDLLARARDVLTNARNAELTEYLQVVQNYGLQAGALTDHLCF
                     DFYDLPQIPHRIGEELGGAARFVIREGECPFCRLVRDEVADPARLVYEDAASVCFAPY
                     ASRSPFELWVVPRHHAADFGTASDAQLVSAADTLQSVLRLLASLGGPAYNLVLHSAPL
                     RERVDDTYHWHWEIHPRLREIAGLEIGTGLPVNPIAPEQVARELLETAREERIETGVA
                     AGPRSWDEFTDR"
     gene            complement(6182..6904)
                     /gene="lipB"
                     /locus_tag="VIF08_00855"
     CDS             complement(6182..6904)
                     /gene="lipB"
                     /locus_tag="VIF08_00855"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY8134561.1"
                     /translation="MAGQSGGLEVLWLGRVRYRDAWQKQHDLVAARRRDEIPDQLLLL
                     EHEPVLTLGRTSDEKHVLASPDELQRRGIELIRTERGGEVTYHGPGQLVGYPIVRLAD
                     RGILLRPLVRALEQAMIDTAAGFGVAAGRIDKLPGCWCDPDSASPRKLGALGLRVEGG
                     VSYHGIALNVTTDLRDFDLIDPCGLPQIEVTSIAREAGWHEDESHPSTASVANAADRF
                     AIAFAARLQEASSPAVLASSAG"
     gene            complement(6955..7428)
                     /locus_tag="VIF08_00860"
     CDS             complement(6955..7428)
                     /locus_tag="VIF08_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY8134562.1"
                     /translation="MTQILDHPITDHSTEQAPLLQQTLLELIELSLQAKQAHWNVVGP
                     TFKPIHEFLDEMTDQYRGWYDDVAERLTAIGVAPDGRSQTVVAGRPFETLPAGSLPTA
                     DIGPLFDTRVSALADRIRHRAGDIGETDMATQDLLIEILRGVEKQRWMLRAHSGI"
     gene            7693..8121
                     /locus_tag="VIF08_00865"
     CDS             7693..8121
                     /locus_tag="VIF08_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134563.1"
                     /translation="MRGFGFIGALGFLVLLFIAGAIGFGLGQNTVPAVGPAPGAPVVY
                     YGHGGFLGFGFFGFLLFLFLIFAVIGIFRRAAWGHRGWGHHGYYGRGWDKGERSNWSD
                     RSMPPMADEMLGRWHREAHGQPTSTDPAGTKPDNQPTSQG"
     gene            8133..8825
                     /locus_tag="VIF08_00870"
     CDS             8133..8825
                     /locus_tag="VIF08_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8134564.1"
                     /translation="MPKVLVVDDAPDIVRLVRDYLERAGFSVLAAANGVDAIRIARAE
                     RPDLIVLDLGLPGRDGLDVTRELRRDPAVSSVPIVMLTARADESDKLVGLELGADDYV
                     TKPFSAKELVARIRAVLRRAQAVAAPAESLQVGDLVIDVPRMAVSVAGADVELTATEF
                     QILLVMARQRGRVFTRAQLLDAVRGEAFEAYERAIDAHIKNIRRKLEPDPATPRYVLT
                     VFGVGYRVAERP"
     gene            8822..10003
                     /locus_tag="VIF08_00875"
     CDS             8822..10003
                     /locus_tag="VIF08_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8134565.1"
                     /translation="MTRDGAPRPERGERSERSERPERPRIKPVWWPEGEEWPPRRWRY
                     GNRHGRWGDRRHRGPRPFGCLFLILILLVAGLLTSAFWSLAAIAGFVSAPPVVTAGGL
                     VVTLLVLVAAGATFASIRRATAPIDDLAAAAERIESGDYSTRVEERGSKSTQGLVRAF
                     NQMSSRLQALDQGRRIFLADVAHELRTPLSVITGQLEAIEDGVYPADAEHLEPIREQI
                     KALEKLIDDMRIVALAEAGGLTLMLAPTDLTDLLTDQLAAFSAQAGAAGVTLLPEIAA
                     GLPFAMADEPRVRQVVANLLANALRHTPAGGRVTLSARASVDGDTLTVEVADTGRGID
                     FELLPRVFDRFVKGQGSAGSGLGLAICRDIIEAHGGDISIASEPSRGTTLTFSLPAVA
                     T"
     gene            complement(10011..10086)
                     /locus_tag="VIF08_00880"
     tRNA            complement(10011..10086)
                     /locus_tag="VIF08_00880"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10051..10053),aa:Gly,seq:ccc)
     gene            11118..11876
                     /locus_tag="VIF08_00885"
     CDS             11118..11876
                     /locus_tag="VIF08_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134566.1"
                     /translation="MAVKFRRRFLGALFAGVLVITACSGGPFLVSRADGEPAPQLLRP
                     VTNQPAPLASCGGHPFAPEALDAPPTDQVGPEFDALRAVFAAHAGEFEGTPEQEPWKL
                     VDKQDDQLLFVADSGSGLLYVFLRRQGDIWKFANMGDCQLHAALGDGAGAARWWLDPR
                     RPFPTADSVVLEILVLEQACASGNYATGRILPPAVVYEAEAITVTIGVRGVGGTCQGN
                     PDTPAVLTLNEPLGDRELLDGFFIPPAPALPPAP"
     gene            complement(11839..12555)
                     /locus_tag="VIF08_00890"
     CDS             complement(11839..12555)
                     /locus_tag="VIF08_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134567.1"
                     /translation="MGADDARAQVAKKILDAALARVTDPPGTNRVRVEDYVTAVAAVT
                     GEAAIVAAGIDIEALDITPGAALFGDQINQVLSGDTTELDKVPPDTIVGILVASLVPN
                     VVALADFGSLEDRYRSVAAHVGKQDWGNVYVSVGAHHAPTIPPIRLAFEMRPVVEEAC
                     AEAGISAAQRHQPCALALASALDQVKGAIDIKLGLKLALEVVFGMAKMAPMSRRAFVE
                     VAGTEAENVREPEAEPARAE"
     gene            complement(12560..13690)
                     /locus_tag="VIF08_00895"
     CDS             complement(12560..13690)
                     /locus_tag="VIF08_00895"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8134568.1"
                     /translation="MTAEPVIASSPIAPRLPDSLDTPALVIDLDIVERNAARMAAAAR
                     EHGIALRPHVKTHKSVALARLQVDAGAAGITVGTLGEAEIMADGGITDIMLAYPLWAA
                     GPKAARLRALAERPGLAFAVGADSVAGVEQLAQAMSGSTARLRVMIEIDPHYGRTGVT
                     PDSVAEIGRAVERAGFELLGLFSHGGHAYAGGDAIAAAARDELEALAIGEEALVAAGM
                     AAPVLSAGSSPTALGVLSAPVNEVRPGTYLIGDRMQVWLGTTPPDGVAIAVAATVVSA
                     AVDGQVVINAGAKTLTKDMAPYMNGYGCIPAYPEGVIERVSDYHGQVRFPAGAPRPAL
                     GETVAVVPNHACPTIDLFDSFAATRSGSSIGWWPVDARGRSG"
BASE COUNT         2131 a         4638 c         4862 g         2248 t
ORIGIN      
        1 caaaggcgag cgtctgtcag ggagcggcgt ggtccttgtc tgactcgtct tcgagctggc
       61 cgaccgtgcg gcgggcggag ggcgagttgg ggcggcggcg ggagcgccgg tcggcggcga
      121 cgaggcggag caggaagatc atcgtctgcc cggtgaagaa aagcagcacg agcgacagga
      181 tgagcaatgc cgccgacacc tccgacggcc acgcccagag cacgctgagc ggggtgagga
      241 ttagcagcgc gaccagcagc gcgatctgaa tccagaagca gcccaggccg ggaccgcgct
      301 cggcgtcgag cggcgcgccc ttgtattcgg gcatggccgg cccggggtgg tcgcgggagc
      361 cgccctcagt cgcgtcggcc gttgcgcgat cggtcatgtg aggcgatggt agtcgcgatg
      421 ggctcacgcg ccggaatgtt cgaaatccgc gaggcaaagg catccgacgc gcgcggcatc
      481 gcagaggttc gcgtccatac gtggcaggag gcctatcgcg acatcatgcc gacggcattc
      541 ctgaacggcc tgagcgtgga cgctgatgaa aagcgctggc gcgacctgct gaaggcgcag
      601 gcgcccaacc gccggatgct agtggccgac tctggcggcc aggttgtggg ttttgtcact
      661 gccggtgtcc tgcgcgaaga gaatgcgcag ccgcgaactg gcgaggtcta tgcgatctat
      721 gtgctgcccg actggtgggg ccagagcatc ggccgtggcc tcctcgccca cgcggaacag
      781 gccttgatcg agcagggcta tgacgcggcc gtgttgtggg tcttggccga caaccggcgt
      841 gcgcgggcct tctacgagcg ggccggctgg catccggacg gtggaaccaa aagcgccacg
      901 ttcggcggcc gtgaagtgga ggaggtgcgc taccggatcg cgctggggag gtcgcgcgtg
      961 gatgagcctg cctgagcgcg ggttgggccg cgcaggccgc gcggcgtaga cggggcaagc
     1021 agcgccagca ccgccatgcg gacggccagg ccgttggcga cctgctcgag gacgagtgac
     1081 cgcggccccc gtgccaccgc gcgagtcacc tcgacaccct cgttgatcgg cccgggatgc
     1141 aggtagcggg cctctggcgc gaagcgcacg agccgctcct cggtgatctg gtagcgggcg
     1201 atgtagtcac cgattgaaag cgcggagccg cgcatgcgct ccttctgcac ccgcaggccc
     1261 atcaccgccg acgcgccatc gagcgcggcc tcgagatccg tctttgtcgt caccggcagc
     1321 tctgagtagc cgcgcagcca ggaatcgggt ccgctgaccc agacatccgc gccggcgcgg
     1381 gtaagcgtcc agacgttgga gcgcgccacg cgcgaatgga ggaggtcgcc gacgatggcg
     1441 atcttgcgcc cgcgcagcgc gtcgcgcgat cccccgaatg cgcggcgcag ggtcagcagg
     1501 tcgagcagcg cctgggtcgg atgggcgtgc caaccgtcgc ccgcgttcac caccgcgccg
     1561 ccgaagtggc gcgccaccac ccatggcgcg ccggcgcggt gatggcgcat cacgatcatg
     1621 tccacgccga gcgcgtcgag gttgcgcacg gtatcgacca gcgtctcgcc cttgaccatg
     1681 gagctcgacg ccggatccac cacgtacgtc tccgcggaca ggccgcgcgc ggccatctcg
     1741 aacgacatgc gcgtccgcgt cgatggctct gcgaagagca gcacgactcg gcagccgaga
     1801 agctcgttgc cagggcgctc cccggcgtcg cgggcgcgtg acgcgttatc gagaactgcc
     1861 tcgatcgtct cgtccgacca ctcgtcgacg tcgagcaggt gccgcggatc agctgtcatg
     1921 ccccgtcctc agccccgggt gcccggcgct cgatgaccac gtcgtcatcg ccgccgtcga
     1981 cctcgcgcag gtggactttg atcacctcgt cgcgcgacgt gggcacgttc ttgccgacgt
     2041 ggtcggcgcg gatgggcagc tcgcgatggc cacgatcgac cagcaccgcc agccgaaccg
     2101 ccttgggccg gccgacgtcg accagggcgt ccagcgcggc acggacagtg cgcccggtgt
     2161 agagcacgtc gtcgacgatg acgatcgtcg atgcctcgag cgcgaagggc aggtccgtcg
     2221 ccttgagcag cggcgccttg gcgacctgcg acaggtcgtc gcgatagaag gtgatgtcga
     2281 ggctgccaac gggcggctgc acgccctcgt gctccttgat cgacgcggcc agccgttcag
     2341 cgatcggcac gccgcggcgc tggatgccga ccagggccag gccgtctgtg ccgccgtgct
     2401 tctcaacgat ctcgtgactg atgcgcacga gggcgcgtcg cacgtcgtcg gtggtcatga
     2461 tctgacggct ggtcacgagc ccagattgta gaaggcggac atggtcccga gagcgggcgc
     2521 gacgctagaa ggcgggcacg accccatcga gcgtcaacgg ttcgagccgc acgccaccga
     2581 cgccgtcatc gccccaggtg cggatgcgca gctcgccgtc ggggttgtca tccgatggcg
     2641 cgacccacac gacccggctc aggccaaggc tgaagccgcg ccgcgcaagc gtggccgaca
     2701 gcaatgcttc cgacgacgcg accgcgcccg tctccggatc gaccgccatc acggcgagcc
     2761 tgccccaggt cgagccgacc gtatcggcca gccagatgcc gagcacctgg ccgtcgagcg
     2821 accagcgcgc ctgccagtca aggaccggcg tcgtagccgt gtccctgccg agatccagct
     2881 cgacccagcc atcgggcagt gccgagggag tggctgtctc agaaggttcg accggggtct
     2941 cagtcgattg cggctcgggt gtcgcggacg gaccttcgct gggcgctggc tcagtcgagg
     3001 ccaccaccgg tgaggccgac ggcgaagcgg gtggcgctgt tggcgggtcc gatggatgcg
     3061 ttgaagccgg agcgtcggtg ggggccggag cgtcggtagc agccggagca tcggtgggag
     3121 tcggagtctc cacgacatcc ggggttgcga ccggctcatt ggccgctgcg aacggatcga
     3181 ttgcggccca gtcgatcatg agcagcgcgc ccgagctcgg cacgggatgg ccactggcga
     3241 aatcgagatc gccgcgccag gcaatcgcgc gcgtgccggc cgggttgacg acgggcagcc
     3301 acagtgcggg cccggcgacc ggccgctcct cggacgtgct gggatccatg acgatcgtgc
     3361 tcaacgacgc gccgtcgctg ccttcgccat taccgacgcc ggccaggcgg ctgatgacga
     3421 tcctgccggt cgaccaggag gcgaaatatg agccgtgatc agtggttacc ggccgcgcga
     3481 ccgcgtcgct gggcgaccag acgtagacat ctggaccgtg gctgccgtcg gcgggcatcg
     3541 cgctgaaggc gagcatctcg ccgctcggtg accaggccgg cggtgcgccg gcactctcga
     3601 cgttctcgag aatcgccacg acgacaggcg gcgcatcgct gggcgtggcc tcgggttggc
     3661 cggggccggt gacagtgaca ggcgtggcac cagggttgac cggtgcaggc gtgccgtccg
     3721 gcggtgtggg atcgtgcgct ccgtcggaca ggagcacgac cgcgatcaca tcgtggcccc
     3781 cgccgctgcc gacgagcgcg agggtgcggc cgctggggct gagggccagg ttgcgcgcgc
     3841 gcacgctggc cggcaggttg accggcgttc tgacaatgcc ctcgtcgaca gcgcagcctg
     3901 ccgcattcgc agggcaggca tgccccacgt cggttcggta gacgtacatg tgcgcgtcat
     3961 cgctgccgac gaacgcaagc tgctgcggcg cgatggcaaa gggggtggcc ggcgcgggca
     4021 cgctcggtga ggcgatcgag acggccgggg tcagctgcat cgcggtgagc ccgacgacca
     4081 cgccgagcga cgcgatggtc gccatcaacg cagccgaggg cccgaacttc cggccacgcc
     4141 gccgccaggt gcggccaaag cgcgaagacc cgcgggcgac ctcgcgatcg agcgcggccg
     4201 aggtgcgcgg ccacaggttg cgcggcacgg ggcggagcgg caaagcgcgc agcagggcgc
     4261 gctgctggcg atagtcgcgg tcggcctcgc ggcacgcggc gcaggcgcgc aggtgagccg
     4321 ccagtgagcg cgtctcggat cgcgtcagtg gtcggtcgag ccgcgcggcg atcagctcgc
     4381 gccagctggc gtgatcagcg cgagcgcggg ccatcttcat cgtcacgcct atggcgcgcc
     4441 tcaacgggcg cggccgcgcg agctcgtttc tcgtcctcta ccgcggagtt cgcgcgctca
     4501 atcgcgagcg cctgacgtaa cgactcgcgg ccgcgcgtca gcagcgcccg cgccgcgacg
     4561 tgcgacacgc ccagcgcatc ggcgagctcg agcccggtca tatcgttcag ctcagacagc
     4621 agcagggctt ggcgctgccg cgcggggatg cgggcgaggg cgcgttcaag gtcacccgac
     4681 agtcgggcgc gcatgaccag ctcttcagcg gacgcggcag tgccgcgcga ctcgccggtc
     4741 cagggcacga agcgcacgat cttgcggcgg cgcagctcgt cgagcgccgt ccgaccggca
     4801 atctggtaca gccaggcgcg agcgcgattg ggatcttcga gcgtgtcgta ggcccggtag
     4861 gccttgacaa aggcttcctg cgtcaagtcg gccgccagct cgtcatctcg gaccattcgg
     4921 cagaggtagg catagatctc cgcgtgatgg gcggcgaata ggcgctcgac gaacgcccga
     4981 gcgctcgctc ggtcgtcgct cactccgggt ccctctccat agagctcgag caccaggccg
     5041 tggttcggct ggtgtgacgg ctcgccgcgg cgtcctgtga cggtatgtgc atcgcgccaa
     5101 ttgtcaggca gggcggttgc aggagcgaat cagcggtcgg tgaactcgtc ccatgagcgt
     5161 ggcccggccg caacacctgt ttcgattctt tcctcgcgtg cggtctcgag cagctcgcgc
     5221 gcgacctgct ccggggcgat tgggttgacg ggcaggcccg ttccgatctc gagtccggcg
     5281 atctcgcgca gacgcgggtg gatctcccaa tgccagtgat aggtgtcgtc gacgcgttcg
     5341 cgcagcggcg cgctgtggag cacgaggttg tatgccggcc cgcccagact ggccaggagg
     5401 cgcaggacgc tctgcagcgt gtccgccgcg ctcaccagct gcgcgtcgct ggccgtgccg
     5461 aagtcggcgg catggtggcg cggcacgacc cacagctcga acggtgagcg cgacgcgtag
     5521 ggcgcgaaac aaacgcttgc cgcatcttcg taaacgagtc gcgccgggtc cgcgacctcg
     5581 tcgcgcacga gccggcagaa agggcattcg ccctcgcgga tcacgaagcg cgcggctccg
     5641 cccagctcct caccgatgcg atgcgggatc tgcggcaggt catagaagtc gaagcacagg
     5701 tggtcggtca gagcaccggc ctgcaggccg tagttctgca caacctgcag gtactcggtg
     5761 agctctgcgt tgcgcgcatt cgtaagcacg tcgcgcgccc gcgcgagcag gtcatacgcg
     5821 atctggggtg acgtctcggc gagcgactcg tggtggccgc gcggcgcgac gatggtctgg
     5881 tacgagccgc tgtcaccgac catcccgagc tccggctcgc gaccggccgg ctgcgcctcg
     5941 cgcccttcct tctctgtgcc cgcgacgatg aacgcgtcct gcttgagcgt ccgtatctgg
     6001 acctgttcgc cgcccgcggc caggtcgcag ttcgggcagt catcggtcgt ctggccgatg
     6061 tgcctggcgg gccggaagaa gcgctgccgc tcgaactcgc ggtctactac gacggcgacc
     6121 caccagccgg tgatggcgtc cttgcgcagt tcgaataggc cgcgcggcat tgttgattcc
     6181 tctagccggc ggaggaagcg aggaccgccg gagaagacgc ttcctgcaga cgcgccgcga
     6241 aggcgatcgc gaagcggtcg gccgcgttgg cgacgctcgc agtggaggga tgcgactcgt
     6301 cctcgtgcca gccggcttcg cgggcgatgc tcgtcacctc gatctggggc aggccgcacg
     6361 ggtcgatcag atcgaagtcc ctgagatcgg tcgtgacatt gagcgcgatg ccgtggtagc
     6421 tgaccccgcc ctcaaccctg agcccgaggg cgcccagctt gcgcggagat gcgctgtcgg
     6481 gatcgcacca gcagcccggc agcttgtcga tccggccggc cgcgacacca aagccagcag
     6541 ctgtgtcgat catcgcctgc tccagagcgc gcaccagcgg acgcagcagg atgccgcggt
     6601 cggcgaggcg cacgatgggg taaccgacca actggcccgg cccgtggtac gtcacctccc
     6661 caccgcgctc cgtccggatg agctcgatcc cacggcgttg cagctcgtcg ggggaggcaa
     6721 ggacatgctt ctcatcggac gtccggccca gggtcagcac cggctcgtgc tcgagcagca
     6781 gcagctgatc ggggatctcg tcgcgccgcc gtgcggccac aaggtcgtgc tgcttctgcc
     6841 acgcgtcgcg atagcggacg cggcccagcc agaggacctc tagcccgcct gattggcccg
     6901 ccatggccgc acgatagcag ggctgccgtg cgcgaccgag gcgagccccc agggctatat
     6961 cccggagtgg gcgcgcagca tccagcgctg cttttctacg ccgcgcagga tctcgatgag
     7021 caggtcttga gtggccatgt cggtttcgcc gatgtcaccg gcgcgatgac gaatgcgatc
     7081 ggcaagcgcg ctcacccgcg tgtcgaagag cggcccgatg tcagccgtcg gcaacgatcc
     7141 cgcaggcagc gtctcgaacg gccgtcccgc gacgaccgtc tggctgcgtc cgtcgggcgc
     7201 cacgccgatt gcggtcaggc gctcggcgac atcgtcgtac cagccacggt actggtcagt
     7261 catctcgtcg aggaactcgt ggatcggctt gaaggtcggc ccgacgacat tccagtgggc
     7321 ctgcttggcc tgcaggctga gctcaatcaa ctcgaggagc gtctgctgga ggagtggtgc
     7381 ctgctcggtc gagtggtcag taatcgggtg gtcaaggatc tgggtcatca ggacgcctcg
     7441 gtgtgctgcg gatgctgctg tcctgaacct acgcgctgcg atccatgcga gcaagtgccg
     7501 accggctcgg agggcggggg tcccgtcgcg tgttcaccgg cgacacgtcg ggccgtagct
     7561 caggctgatc caggcgggcc aactgcacag cgacggcgtc gcgcgttcac aacttccaca
     7621 cgtcctggcg cgaggctcct tacgtgagcg gcgcagaggt cgccgcgcaa acgacaaagg
     7681 agcaatcacg cgatgcgtgg ctttggtttc atcggagccc tggggttcct ggtcctgctg
     7741 ttcatcgccg gggcgatcgg cttcggtctt ggtcagaaca ccgttccggc ggttgggccc
     7801 gcgccgggcg cgccggtcgt ctactacggc cacggcggtt tcctgggctt cggcttcttc
     7861 ggcttcctgc tgttcctgtt cctgatcttt gcggtgatcg gcatcttccg ccgcgcggcg
     7921 tggggtcatc gcggctgggg ccatcacggc tattacggtc ggggctggga taagggtgag
     7981 cggtcgaact ggtctgatcg gtcaatgccg cccatggccg acgagatgct gggtcgctgg
     8041 caccgcgagg cacacggcca gcccacgtcg accgatccgg ccggcacgaa gcccgacaac
     8101 cagcccacct cccagggctg acaatcaccg gcgtgcccaa ggttctcgtc gtcgatgacg
     8161 caccggatat cgtgcgtctc gtgcgcgact acctcgagcg cgccggtttc tccgttcttg
     8221 ccgccgcgaa cggggtggac gcgatccgca tcgctcgcgc agagcgccct gacctgatcg
     8281 tgctcgacct gggcttgcct ggtcgcgacg gactcgacgt gacgcgcgag ctgcgccgcg
     8341 acccggctgt ctcgtcggtg ccgatcgtga tgctcaccgc ccgggccgac gaatcggaca
     8401 agctcgtcgg gctggagctg ggtgccgacg attacgtgac caagccgttc agcgccaagg
     8461 agctggtggc gcggatacgc gcggtgctgc gccgggccca ggcagtggct gcgcctgcag
     8521 agtcgctgca ggtaggcgac ctcgtgatcg atgtcccgcg gatggcggtc tcggtcgccg
     8581 gcgctgatgt cgagctgacc gcgacggagt tccagatcct gctcgtgatg gcccgccagc
     8641 gcggccgcgt cttcacccgc gcacagctgc tcgacgcggt tcgcggcgag gcattcgagg
     8701 cgtatgagcg ggcgatcgac gcgcacatca agaacatccg gcgcaagctc gagccggatc
     8761 cggccacgcc acgctacgtg ctgaccgtgt ttggcgtggg ctaccgcgtt gcggagcgtc
     8821 catgacgcgc gacggcgcgc cgcggccgga gcggggagag cggtcggagc ggtcggaacg
     8881 gccggagcgg ccgcgcatca agcccgtctg gtggcctgaa ggcgaggagt ggccgccgcg
     8941 caggtggcgt tacgggaacc gtcacggtcg ctggggcgat aggcgccacc gcgggccgcg
     9001 gccgttcggc tgcctgttcc tcatcctgat cctgctggtc gccgggttgc tgacgtcggc
     9061 tttctggtcg ctcgccgcga tcgccgggtt cgtcagcgcg ccaccggtcg tcactgccgg
     9121 tgggctcgtg gtgacactgc tcgtccttgt cgcggccggc gccacgttcg cgtcgatccg
     9181 acgggcgaca gcgcccatcg acgatctcgc cgcggccgcc gagcgcatcg agagcggtga
     9241 ctactcgacg cgcgtcgagg agcgcggctc aaagtcgacg cagggactgg tgcgcgcctt
     9301 caaccagatg agcagccggc tgcaggcact cgatcaggga cggcgcatct tcctcgctga
     9361 cgtggcgcac gagctgcgta ccccgctgag cgtcatcacc ggccagctcg aggcgatcga
     9421 ggatggcgtc taccccgccg acgcggagca cctcgagccc atccgcgagc agatcaaggc
     9481 gctcgagaag ctgatcgacg acatgcgcat tgtggcgctg gcagaggccg gcgggctgac
     9541 gttgatgttg gcaccgaccg atctgaccga cctcctgacc gaccagctgg ccgcgttctc
     9601 tgcgcaggcc ggtgcggctg gcgtcacgtt gctgcccgag atcgccgccg ggctgccgtt
     9661 cgccatggcc gatgagccgc gcgtccgcca ggtcgtcgcg aacctgcttg ccaatgcgct
     9721 gcgccacacg cccgccggtg gtcgcgtgac gttgagcgcc cgcgcctctg tagacggcga
     9781 caccttgacc gtcgaggtcg cagacacggg gcgaggcatt gactttgagc tgctcccgcg
     9841 cgtcttcgac cgattcgtca aggggcaggg atcggccggg tcggggttgg gtctggcgat
     9901 ctgtcgcgac atcatcgagg cgcacggcgg cgacatctcg atcgcgtcag aaccgtcgcg
     9961 cggcacaacg ctgacgttca gcctgccggc ggtcgcgacc taaaggaaag tggagcggga
    10021 gaaggggctc gaaccctcgg cctgttgctt gggaagcaac cgctctgcca actgagctac
    10081 tcccgccgag aaccgaaatt gtacgagagg agtccttgcg ctaggccgcc ctgatctgag
    10141 cgtttgttcg tcgacggtgg caattggcac accgaacctc gcacttaccg atctcaagtt
    10201 ggattgcgag ccacgtccta tgcccaacca tcgttccgat ggccttgctc ttcagcgagg
    10261 gatcgacgtg gtcgaaatcc agcgtcgcga cgttcgtttc tccacagtcg acacagggat
    10321 gcgccaacaa gtagtgatgg agccgagcct tagcgtcagc agtcttccgt cgggtttgcg
    10381 cgatggcccg cctgaagtac tcgtcgcgat tgatttggta gtgctcccgt cgatacgcgg
    10441 catggcattt cctgcagtgg ccgttgacct tgcctgtggt cgcgtccttg aaggcaaact
    10501 ccgacagcgg cttcagctga ttgcaccaag cgcaagccat cagaaactca gggtcggagg
    10561 gcgccctgcc cacggcaggc ggtcgaactg gggatggaga tccatccggc tttgttttga
    10621 gtttggccca gccaaactgc gctgcagtac gttttcgatg gcaattcgcg caccggacat
    10681 cgcatttctc gatttctcga aggactgacc gccagcccac gacctcggcg agacgcgaca
    10741 ctggcagtcg cttttctgct ggatcgcgat ggtcgaactc caggatcgcc cgggatgtct
    10801 cgccgcagtc cacacaaggg tgctgattta ggtactcagt tatccgggct cgcactcgtg
    10861 cccgaccgtt gtcgcccctg aggcgacgtc gtgctttgta cgtctctgcg ttgttgaggt
    10921 agtgctgatg tgagtaagcc tgtgcgcatg atcggcacca cgatcggcga attccgcgcg
    10981 ctctgttctt ccattggaaa ccttcgaccg gctgctcgac gccacaccgc gaacatctct
    11041 tcacgctcaa gtccgggacc gtcgtagtgt ccatatagca cggatattct atatggccga
    11101 gcgcctattc tcaataggtg gccgtgaaat tcaggcgacg cttcctcggt gcgctattcg
    11161 cgggcgtttt ggtgattacc gcatgctcgg gcggaccttt cttggtcagt cgcgccgacg
    11221 gcgagccggc tccgcagctg ctgaggccgg tcacgaacca gccggctccg ctggcgagct
    11281 gcggcggtca ccccttcgcg cccgaagctc tggatgcacc gccaacagat caggtcgggc
    11341 ccgagttcga cgcattgcgt gctgtcttcg ctgcacatgc tggcgagttc gagggaacgc
    11401 cggagcaaga gccctggaag ctcgtcgaca agcaggacga ccaactgctg ttcgtcgcgg
    11461 actcagggag cggcctgctg tacgtcttct tgcgacgtca aggcgatata tggaagttcg
    11521 ccaacatggg cgattgccag ctgcatgccg cgctcggcga tggcgctggg gcagcccgct
    11581 ggtggctgga tccgcgccgt ccattcccga ccgcagactc ggtggtgctt gagatcctgg
    11641 ttcttgaaca ggcctgcgcc agcggcaact acgcgaccgg tcgaattctt ccgccggccg
    11701 tggtctacga ggcggaggcg ataacggtca cgattggtgt ccgcggggtt ggcggcacgt
    11761 gtcagggcaa tccggacacg cccgcggtat tgacgttgaa tgaaccgctc ggcgatcgcg
    11821 agctgctcga cgggtttttc attccgcccg cgccggctct gcctccggct ccctaacgtt
    11881 ctctgcctct gtccccgcga cttcgacaaa ggcgcggcgt gacatgggcg ccatcttggc
    11941 catgccgaac acgacctcga gcgcgagctt cagcccgagc ttaatgtcga tcgctccctt
    12001 gacctggtcc agcgcactcg ccaatgccag cgcgcacggc tggtgacgct gcgcggcgct
    12061 gatgccggcc tccgcgcagg cttcttcgac gacgggtcgc atctcgaacg ccagtcggat
    12121 cggcggaatc gtcggcgcat ggtgggcgcc gaccgatacg tagacgttgc cccaatcctg
    12181 cttgccgacg tgggccgcaa ctgagcggta gcggtcctcg agcgagccaa agtcggccag
    12241 cgcgacgacg ttgggcacga gcgaggccac gaggatgccg acgatagtgt cgggcggcac
    12301 cttgtcgagc tcggtggtgt cgccgctgag gacctggttg atctggtcgc cgaacaacgc
    12361 ggcgccgggc gtgatgtcga gcgcctcgat gtcgatgccc gcggcgacga tcgctgcttc
    12421 accggtgacc gcggccacgg ccgtgacgta atcctcgacg cgcacgcggt ttgtgccggg
    12481 cggatccgtc acccgcgcca gggccgcgtc gaggatcttc ttggctacct gcgcgcgggc
    12541 gtcatccgct cccaacgcgt cagccgctcc ggccgcgcgc gtcgaccggc caccacccga
    12601 ttgacgagcc ggagcgggtg gctgcgaagg agtcgaacag gtcgatcgtc ggacaggcgt
    12661 gattcggcac gacggccacg gtctcgccca gcgcggggcg cggtgcaccg gccgggaagc
    12721 gcacctggcc gtggtagtcg gagacccgct cgatgacgcc ctccgggtag gcgggaatgc
    12781 agccgtagcc gttcatgtac ggcgccatgt ccttggtgag cgtcttggcc ccggcgttga
    12841 tcaccacctg gccgtcgacg gccgcgctga cgaccgtcgc agcgaccgcg atggcgacgc
    12901 cgtctggcgg agtggtgccc agccagacct gcatacggtc gccgatcagg taggtgccgg
    12961 ggcgcacttc gttgaccggc gcgctgagca ctcccaaggc cgtcggcgat gagcccgcac
    13021 tcaacaccgg cgcggccatc ccggccgcga cgagcgcttc ctcgccgatc gccagtgcct
    13081 cgagctcgtc gcgcgccgcc gcggcgatcg cgtcaccgcc cgcataggcg tgaccgccgt
    13141 ggctgaacag gccgagcagc tcgaacccgg cgcgctcgac cgcccggccg atctcggcga
    13201 ccgaatccgg cgtgacgccc gttcggccgt aatgcgggtc gatctcgatc atcacccgca
    13261 gccgggcggt cgagccgctc atcgcctggg ccagctgctc cacccccgcg accgaatccg
    13321 cgcccaccgc gaacgccagg ccgggccgct cggccagggc gcgcagccgg gccgccttgg
    13381 gtccggccgc ccagagcggg taggcgagca tgatgtccgt gatcccgccg tcggccatga
    13441 tctccgcctc gcccagggtg ccgacggtga tcccggctgc gccggcgtcc acctgcagac
    13501 gcgccagcgc gaccgacttg tgggtcttga cgtgtggccg cagcgcaatc ccgtgctcgc
    13561 gcgccgccgc ggccatccgc gccgcgttgc gctcgacgat gtcgagatcg atgaccagcg
    13621 caggcgtatc gaggctgtca ggcagacgcg gagcgatggg gctggaggcg atcaccggct
    13681 cggctgtcaa ggcgccgagg ctaacaccga cacggttccc gaacgacggc gccgcccggc
    13741 ccgtctggcg gcccagttcg tccgtttggc ggggccttcg tgccaaacgg cacgcgtccg
    13801 gctcgcggcc gcccaccgag gattgggcag aagggagttc cggttgcaga accagcccgt
    13861 gaagacacct gacggcgac
//
LOCUS       DATBRX010000025         7340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3135368, whole genome shotgun sequence.
ACCESSION   DATBRX010000025 DATBRX010000000
VERSION     DATBRX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135368"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VIF08_00900"
     CDS             complement(<1..665)
                     /locus_tag="VIF08_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY8134569.1"
                     /translation="MKLIIDVDTGIDDALALLYVLASPEAELLAVTCTSGNVEAGQVA
                     ENTIALLDLCGRSDIEVGLGQREPLQRPLMTAPETHGPKGVGYAVLPPASRSVSERHS
                     ADLIVEEVRRAPGEVTLITLGPLTNLAAALEREPKLPSLARNLVMMAGSYRSPGNTAP
                     TSEWNVACDPEAMQAVLSAWESAEGVGRPVALGLDVTERAKFLPDHLARLHAAAGNPS
                     TNRV"
     gene            683..1375
                     /gene="merB"
                     /locus_tag="VIF08_00905"
     CDS             683..1375
                     /gene="merB"
                     /locus_tag="VIF08_00905"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046413 - organomercury catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HEY8134570.1"
                     /translation="MAVEGAPTLAQRVRLYVFEHFLEHAVPPLVEQLMTEFSLTRAET
                     VTVLREVAESRGVAVVKGTSRVLMAWPFSAIATPFAVRTGGKRYYANCSWDAIAFHAM
                     LDQAAVRIDSYCHHCARPIEIELEDGRATMVEPAGTIVYLALRPTQWWEDIILTCSNA
                     MVFFCSAEHRDASGLVPDDQPGASLTPDQAHALGVPLYADRLKVDYQRPGRDALNAHF
                     ASLGLTGPYWQV"
     gene            1380..1871
                     /gene="purE"
                     /locus_tag="VIF08_00910"
     CDS             1380..1871
                     /gene="purE"
                     /locus_tag="VIF08_00910"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY8134571.1"
                     /translation="MAGQSFVAIIVGSRSDLEVVQKAADTLNSLGVGNELRVISAHRA
                     PDLLESYVAGALERGAKVFICAAGLAAHLPGVVASKTTLPVIGLPMPGNLAGGLDALL
                     SIVQMPKGVPVATVGVGQAENAALLAAQMLGLSEPAVAQAVADHRAAARKQIEDDPTN
                     QPG"
     gene            complement(1873..2220)
                     /locus_tag="VIF08_00915"
     CDS             complement(1873..2220)
                     /locus_tag="VIF08_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134572.1"
                     /translation="MATQGDVRRIALGLPDVVAEGSTFRVDGHLVAWPWLERIDPKKA
                     RVANPEVLVVRVASELDKHALIDLDPAVFFTETHFDGYAMVLVRLAAIDDALLAKLLA
                     DSWTLAKAKRKRR"
     gene            2247..2624
                     /locus_tag="VIF08_00920"
     CDS             2247..2624
                     /locus_tag="VIF08_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134573.1"
                     /translation="MDLDRQELPPPRANEPPQRVPEVKPPLLGEWFIYLSVVVLICGV
                     IAITALELGTPLEAPIVKWLSIIGAAVLLLVAGDAAIRTWRSAFAWMPVNRPRALQRL
                     VWTAVLLGVLVGCIALIVFMVGR"
     gene            2621..3223
                     /locus_tag="VIF08_00925"
     CDS             2621..3223
                     /locus_tag="VIF08_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8134574.1"
                     /translation="MTQSAPKGVPDWLAAVVNYCSRCGSPLSHGPVANETRDRLACTS
                     CGFIAYLNPRLVVSTLPVTPDGRLVLIRRGIEPGYGSWAQPGGFLEIDETAHEGAVRE
                     TLEETGYIVDPDEVIGVYTRPLAAIVVLVWEARVVGGAPAISAETLEVRSFGADEIPW
                     SELAFQTTTWAIRDWVRLRHPTIGHPESTDDEWRWPGESA"
     gene            complement(3238..3534)
                     /locus_tag="VIF08_00930"
     CDS             complement(3238..3534)
                     /locus_tag="VIF08_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134575.1"
                     /translation="MPLPAALGVLALAHLFDWASFLVMITRHGLGAEANPIVVTLVEQ
                     TGVPGVTLAKVATVTFAALLAVLIAPQRRRMAMALLTFGAAAGLVGGISNIATL"
     gene            complement(3609..5069)
                     /locus_tag="VIF08_00935"
     CDS             complement(3609..5069)
                     /locus_tag="VIF08_00935"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8134576.1"
                     /translation="MAVSAAPRQAKMFIGGEHVDALDGQTFEVTNPATGKVIAHAPLG
                     GKADVDRAVEAAQKAFDGPYGKWSAAKRGRTLQKFADLVKKANEELAQIDRENVGKPI
                     VASRGESFAVSLVLEYYAGAANKHFGETIPTSIPGFDFTLREPIGVVGLIVPWNFPQN
                     MAAWKLGPALATGNTCVLKPASWTPLSAIRLAELALEAGFPPGVVNVVTGPGGTTGAA
                     LAAHPGVGKVAFTGETTTGQEIMRLAANNVKRISLELGGKSPNVVFADADLEKFAAAV
                     PNSVFDNAGQNCTARSRILVERSVHERVVELVAQQTAKLVVGDPADEKTQMGSLVSMR
                     QRERVTELIASARDEGANVVFGGEPPEDAALANGAFLMPTVIDGANSDMRFIRDEIFG
                     PVVGIIPFDSEEEAVRLANATPYGLAGSVWTRDIGRAIRVARGIKSGTISINSNETIH
                     TEAPFGGYKMSGLGRELGMHALDEYTQVKNVFVDLS"
     gene            complement(5075..5839)
                     /locus_tag="VIF08_00940"
     CDS             complement(5075..5839)
                     /locus_tag="VIF08_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134577.1"
                     /translation="MHDAPSAARLPALRPAEQPALPAATQPELAELFAFMAEAELRFE
                     SLRMRIVDKRTTSNGEEVETHDIWLRHPGHAKVVSSRGDPSERDYDVWAADGDKVHTY
                     DAKGNVTTERRLPVRPVGAESVDLPPWSRIYLPVTTLPAESLAEAFVHPRGFCHNVLM
                     TGVVYPRGTQKMANGREAILLRCDHPRTSHVLTDRPDHWLEVGVDLQTGIVVLLAEHV
                     AETMTRHAEAVSVSLDERIPDEAFALHVSADTRRIY"
     gene            5930..>7340
                     /locus_tag="VIF08_00945"
     CDS             5930..>7340
                     /locus_tag="VIF08_00945"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEY8134578.1"
                     /translation="MIQAPDAAQTRTEKDPLGPLEVPADAYFGVQTQRAIQNFPISGR
                     RPDVALVRAAVQVKKAAARANRKTGRLPQNIADAIIQAADEVLGLREPLDAALQALLI
                     DNFRVDPFQAGAGVSHNMNTNEVLANRAIEILAAAGVGSGNRGDYSMVNPNDHVNMAQ
                     STNDVFPTSMRVATLDKVRDFLPALDQLAAAFEDRAQAFDGVLKSGRTHMQDAVPIRL
                     GQEFAAYALTTRRARAAIASAAEGLLEQNIGATAVGTGLNAEPEYIKLVVANLSEQTG
                     HKLRTAEQLVHATQSMAPMLAVSAALRGLAVDLAKIAEDLLLMSSGPRTGFAEIKLPA
                     KQPGSSIMPGKVNPVMVENLSMICYHVIGADTAVAWAASRGQLELNVMMPLMAHELLE
                     SLTILTTGCRQFARECVAGIEADTERAAWLLEQSSAMATPLAPYIGYALAADIAKEAV
                     KTGKTIRQLVVEKGVFTQEE"
BASE COUNT         1157 a         2529 c         2459 g         1195 t
ORIGIN      
        1 acccggttgg tcgatgggtt gccggcggct gcgtgcagtc gggcgaggtg gtcgggcagg
       61 aatttcgccc gttccgtgac atcgagcccc aaggcgaccg gccgaccgac gccctccgcg
      121 gattcccaag cggacaggac cgcctgcatc gcctccggat cacacgcaac attccactcg
      181 ctggtcggcg ccgtgttgcc cggggagcgg tacgagccgg ccatcatcac caggttgcgc
      241 gcgaggcttg gcagcttggg ctcacgttcg agcgcggccg cgaggttggt cagcggcccc
      301 agggtgatca gcgtcacctc acccggcgcg cggcggactt cttcgacgat caggtcggcc
      361 gagtggcgct cgctcacgct gcgcgacgct ggcggcagca cggcgtagcc gacgcccttc
      421 ggtccgtgcg tctcgggcgc ggtcatgagc ggccgctgca gtggctcgcg ctggcccaac
      481 cccacctcga tgtcacttcg cccacacagg tccagcaggg cgatcgtgtt ctccgcgacc
      541 tggccggcct cgacgttgcc gctcgtgcag gtaacggcga gcagctcggc ttcaggcgac
      601 gcgaggacgt acagcagagc cagcgcgtcg tcgatcccgg tgtcgacgtc gatgatcagc
      661 ttcacgccgt tacgctagcg ccatggccgt cgaaggagcg ccaacgctgg ctcaacgcgt
      721 gcgcctgtac gtgttcgagc acttcctcga gcacgccgtg ccgccactgg tcgagcagct
      781 catgaccgag ttcagcctga cgcgcgccga gactgtgacc gtgctgcgcg aggtcgccga
      841 gtcgcgcggc gtggcagtgg tcaagggcac ctcgcgcgtc ctgatggcgt ggccgttcag
      901 cgcgatcgcg acgccattcg ccgtccggac gggcggcaag cgttactacg cgaactgctc
      961 gtgggacgcg atcgccttcc acgcgatgct cgaccaagca gcggtacgca tcgattcgta
     1021 ttgccaccat tgcgcgcgac cgatcgagat cgaactggaa gacggccggg caactatggt
     1081 cgagcctgca ggcacgatcg tttaccttgc gctacgaccg actcagtggt gggaggacat
     1141 catcctgacc tgctcgaacg cgatggtctt cttctgctcg gccgagcatc gcgatgcctc
     1201 aggcctggtc cccgacgacc aacccggtgc ctcgctgacg cccgatcaag cccacgccct
     1261 cggggtgccg ctgtacgccg atcggctcaa ggttgactac cagcggcccg gccgcgatgc
     1321 gctcaacgcc cacttcgcgt cgctcggcct gaccggcccg tactggcagg tctgacgcga
     1381 tggccggtca gtcgttcgtc gcgatcattg ttggcagccg ctccgacctc gaggtcgtgc
     1441 aaaaggcagc cgacacgctc aattctcttg gcgtcggcaa cgagctgcgc gtcatctccg
     1501 cccaccgcgc gcccgatctg ctggagagct acgtggccgg tgcgctcgag cgtggcgcca
     1561 aggtgttcat ctgtgccgcc ggcctggccg cgcacctgcc cggcgtcgtg gcgtccaaga
     1621 cgactctgcc cgtgatcggc ctgcccatgc cgggcaacct ggccggcggc ctcgacgcgc
     1681 tgctctcgat cgtgcagatg cccaagggcg tgcccgtcgc gacggtcggc gttggccagg
     1741 cggagaacgc ggcgctcctc gcggcacaga tgctgggcct gagcgagccc gccgtcgccc
     1801 aagcggtcgc cgaccaccgc gccgccgcgc gcaagcagat cgaggacgac ccgaccaacc
     1861 agccgggctg aatcagcggc gcttacgttt ggccttggcg agggtccacg agtcggccag
     1921 cagctttgcc agcagggcgt cgtcgatcgc ggccagccgg accagaacca tggcgtagcc
     1981 gtcgaagtgc gtctcggtga agaacacggc cgggtccagg tcgatcaaag catgcttgtc
     2041 gagctcgctc gcgacgcgca ccacgagcac ctctgggttc gcgacgcgcg ccttctttgg
     2101 gtcgatccgc tccagccagg gccaggcgac caagtgccca tcgacgcgaa atgtcgaccc
     2161 ctcggcgaca acatccggca ggccaagggc gatccgacga acgtcaccct gggtggccat
     2221 ctcaagtccg atcctagact gagcccgtgg acctcgatcg ccaggagctg ccgccgccgc
     2281 gcgccaacga gccgccgcag cgcgtgccgg aagtcaagcc gcccctgctg ggcgagtggt
     2341 tcatctatct ctcggtcgtc gttctgatct gcggcgtcat cgccatcacg gcgctcgagc
     2401 tgggcactcc gctcgaggcg cccatcgtca agtggctgtc aatcatcggc gcggccgtcc
     2461 tccttctggt cgccggcgac gcggcgatca ggacgtggcg ctccgctttc gcttggatgc
     2521 cggtcaaccg gccccgtgcg cttcagcggt tggtgtggac ggcggtcctc cttggcgtgc
     2581 tggtgggctg catcgcgttg atcgtcttca tggtcggtcg gtgacccaat ccgcaccaaa
     2641 gggcgtgccc gactggctcg cggccgtcgt caactactgc tcgcgctgcg gctcgccgct
     2701 cagccacggc cccgtcgcca acgaaacaag agaccgcctg gcgtgcacga gctgcggctt
     2761 catcgcctac ctcaacccgc gcctggtggt gagcacgctg cccgtgacgc ccgatggccg
     2821 gctcgtcctg atccggcgcg gtatcgagcc cggctacggc agctgggcgc agccgggcgg
     2881 cttcctcgaa atcgacgaga ccgcccacga aggcgccgta cgcgagacgc tcgaagagac
     2941 cggttacatc gtcgatcccg acgaggtaat cggcgtctac acccgcccgc tggcagccat
     3001 cgtcgtgctg gtgtgggaag ctcgggtagt cggtggggcg cccgccattt cagctgaaac
     3061 gcttgaagtc agatcgttcg gcgcggacga gatcccgtgg tcggagctcg cgttccagac
     3121 gacgacctgg gcgatcaggg actgggtccg actgagacac cccaccatcg gccatccgga
     3181 atctacggac gacgagtggc ggtggcccgg cgagtctgcc tagccggccg aagtgaccta
     3241 gagcgtcgcg atgttgctga taccgccgac cagacccgcg gcggcgccga aggtcagcag
     3301 cgccatcgcc attcgccggc gctgcggggc gatgagcacc gcgagcagcg cagcgaaggt
     3361 gacggtcgca accttggcca gcgtgacacc cggcacgccc gtctgttcga ccagtgtgac
     3421 cacgatcggg ttggcctcgg cacccaggcc gtggcgcgtg atcatgacca ggaacgacgc
     3481 ccaatcgaag aggtgggcaa gcgcaaggac gccgagcgcg gcgggcaggg gcatgggacg
     3541 attgtcctag tccggtgtag tactgtcgag agccgtgcag tgcggtgcgt tgacaccgcg
     3601 caccagcctc agctcaggtc gacgaagacg ttcttgacct gcgtgtactc gtcgagcgcg
     3661 tgcatcccca gctcgcggcc gaggccgctc atcttgtagc cgccgaacgg cgcttccgtg
     3721 tggatggtct cgttgctgtt gatcgagatc gtgccgctct tgatgccgcg cgcgacgcgt
     3781 atggcacgac cgatgtcgcg cgtccagacg ctccccgcca gcccgtaggg cgtagcgttg
     3841 gccagccgga ccgcctcctc ttccgagtcg aacgggatga tgcccacgac cggaccgaag
     3901 atctcatcgc ggatgaagcg catgtcactg ttcgcgccgt cgatgacagt cggcatcagg
     3961 aacgcgccgt tagccagggc tgcgtcttca gggggctctc cgccaaacac cacgttcgcg
     4021 ccctcatcgc gggcgctggc gatcaactcc gtaacgcgct cgcgctggcg catgctgacg
     4081 aggctgccca tctgggtctt ctcatcggcc gggtcaccga ccaccagctt ggcggtctgc
     4141 tgcgccacga gctccaccac gcgctcatgg accgaccgct cgaccaggat gcggctgcgc
     4201 gccgtgcagt tctgaccggc gttgtcgaag acgctgttcg ggacggccgc ggcgaacttc
     4261 tcgagatccg cgtcggcaaa cacgacgttg ggcgacttgc cgcccagctc gagtgagatc
     4321 cgcttcacgt tgttggccgc gagtcgcatg atctcctggc cggtcgtggt ctcaccggtg
     4381 aacgcgacct tgccgacgcc cggatgcgcc gcgagggcag cgccggtggt gccaccgggg
     4441 ccggtcacaa cgttgacgac gcccggcggg aatccggcct caagcgcgag ctccgccagg
     4501 cggatcgccg agagcggcgt ccaactggcc ggtttcagga cgcacgtgtt gccggtggcg
     4561 agcgccggcc ccagcttcca cgcggccatg ttctgcggaa agttccaggg cacgatcagc
     4621 ccgaccaccc cgatcggctc gcgcagcgtg aagtcgaagc ccgggatcga cgtcgggatg
     4681 gtctcgccga agtgcttatt cgcggcgccc gcgtagtact cgagcacgag gctgacggcg
     4741 aagctctcgc cgcgcgaggc gacgatcggc ttgccgacgt tctcgcggtc gatctgggcg
     4801 agctcctcgt tggctttctt gaccaggtcg gcgaacttct ggagcgtccg gccgcgcttc
     4861 gccgcagacc acttgccata cggcccatcg aaggccttct gagcggcctc gaccgcccga
     4921 tccacgtcgg ctttgccgcc cagtggcgcg tgggcgatga ccttacccgt ggccggattg
     4981 gtgacctcga atgtctggcc gtcgagagcg tcgacgtgct cgccgccgat gaacatcttc
     5041 gcctggcgtg gcgccgcgga aaccgccatg agccctagta gatccgccgc gtgtcggccg
     5101 agacgtggag ggcgaacgcc tcgtcgggga tgcgctcgtc gagactgacg ctgacagcct
     5161 cggcgtggcg cgtcatggtc tcggccacgt gctccgccag taggacaaca atgcccgtct
     5221 gaaggtcgac ccccacttcg agccagtggt cgggtcggtc ggtcagaaca tggctcgttc
     5281 gcggatgatc gcagcgcagc aggatcgcct cgcggccgtt ggccattttc tgcgtcccgc
     5341 gcggataaac cacacccgtc atgagcacgt tgtggcagaa gccacgtgga tggacaaagg
     5401 cctccgcgag cgactccgca gggagcgttg tcactggcag gtagatgcgc gaccacggcg
     5461 gcaggtcgac agactcggcg ccgacgggtc gcaccggtag ccgtcgctcc gttgtcacgt
     5521 tgcccttggc gtcgtaggta tggaccttgt cgccgtcagc agcccagacg tcgtagtcgc
     5581 gttcgctggg gtcgccgcgg ctgctgacca ccttggcgtg gccggggtgg cgcagccaga
     5641 tgtcgtgcgt ctcgacttct tcgccgttcg acgtcgtgcg cttgtcgacg atgcgcatgc
     5701 gcagcgactc gaagcgcagc tcggcctcgg ccatgaacgc gaacagctcg gccagctcgg
     5761 gctgagtggc tgcgggcagg gcgggctgct cggccggccg cagcgcgggc aggcgggccg
     5821 cagatggtgc gtcgtgcatc ggtgagcgat tgtagtgcgg caggccgctc gagcgactga
     5881 actccgggtg gaaggggctc accaacggcg gagacgtaca ctcccatcga tgatccaggc
     5941 acctgacgcc gcgcagacgc gcaccgagaa ggatccgctc ggtccgctcg aggtgccggc
     6001 cgacgcctac ttcggcgtcc agacccagcg cgcgatccag aacttcccga tttccggccg
     6061 acgcccggat gtggcgctcg tccgcgccgc ggtgcaggtc aagaaggccg ccgcgcgtgc
     6121 gaaccgtaag accggccgcc tgccgcagaa catcgccgac gcgatcatcc aggccgcgga
     6181 cgaggtcctg ggcctgcgcg agccactgga tgcagcgctc caggcgttgc tgatcgacaa
     6241 cttccgcgtc gacccgttcc aggccggcgc cggtgtgtcg cacaacatga acaccaacga
     6301 ggtgctggcc aaccgcgcga tcgagatcct ggccgcggcg ggagtgggga gcggtaatcg
     6361 gggcgactac tccatggtca accccaacga ccacgtcaac atggcccagt cgaccaacga
     6421 cgtcttcccg acttcgatgc gcgtcgcgac gctcgacaag gtgcgcgact tcctgccggc
     6481 gctcgaccag ctcgcggccg cgttcgagga ccgcgcgcag gccttcgatg gcgtcctcaa
     6541 atccggtcgc acgcacatgc aggacgcggt gcccattcgc ctgggccagg agttcgccgc
     6601 gtacgcgttg accacccgtc gcgcgcgcgc cgccatcgcc agcgcggcgg agggcctgct
     6661 cgaacagaac attggcgcga ccgcggtcgg caccggactg aacgcagaac cggagtacat
     6721 caagctcgtg gtcgccaacc tgtccgagca gaccggccac aagttgcgca ccgcggagca
     6781 gctcgtgcac gcgacgcaat caatggcgcc catgctcgcc gtgtcggcgg cgctgcgcgg
     6841 ccttgccgtc gacctggcga agatcgcgga agacctgctg ctgatgtcgt cggggccgcg
     6901 gaccggtttc gcggagatca aattgcccgc caagcagccg gggtcgtcga tcatgcccgg
     6961 caaggtcaac ccggtcatgg tcgagaacct ctcgatgatc tgctaccacg tgatcggcgc
     7021 cgacaccgcc gtcgcttggg cggccagccg cggccagctg gagctcaacg tgatgatgcc
     7081 gctgatggcc cacgagcttc ttgagtcgct cacgatcttg acgacgggct gccgccagtt
     7141 cgcgcgcgaa tgcgtggccg gcatcgaggc cgacactgag cgcgccgcgt ggctgctcga
     7201 gcagtcctcc gccatggcca cgccgctggc gccctacatc ggctatgccc tggccgccga
     7261 tatcgccaag gaagccgtca agaccggcaa gacgatccgc cagttggtcg tcgagaaggg
     7321 cgtcttcacc caggaagagg
//
LOCUS       DATBRX010000026         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1459326, whole genome shotgun sequence.
ACCESSION   DATBRX010000026 DATBRX010000000
VERSION     DATBRX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1459326"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VIF08_00950"
     rRNA            complement(<1..149)
                     /locus_tag="VIF08_00950"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(430..615)
                     /locus_tag="VIF08_00955"
     CDS             complement(430..615)
                     /locus_tag="VIF08_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134579.1"
                     /translation="MSNLVIEMLLRLVKPLLAVLLGGLVYWGATGPGGAPGSVELALL
                     SWLSGAAFILLAQEGPL"
     gene            complement(647..1276)
                     /gene="recR"
                     /locus_tag="VIF08_00960"
     CDS             complement(647..1276)
                     /gene="recR"
                     /locus_tag="VIF08_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY8134580.1"
                     /translation="MAALIEPVARLIEALSRLPGVGPKTAQRLTYHLLRAPESEARAL
                     ASALVAVRDEVVYCETCFNISTGPQCPICADPSRDQTRVCVVEEPLDVLALERTGAFK
                     GRYHVLHGAISPMDGIGPDRLRVRELLERAADRSRDGDPYQEVVLATNPTLEGEATAM
                     YLAERLANDVGAITRIARGIPVGGDLEYADEVTLVRAMQGRRAFEVNER"
     gene            complement(1291..1602)
                     /locus_tag="VIF08_00965"
     CDS             complement(1291..1602)
                     /locus_tag="VIF08_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY8134581.1"
                     /translation="MGYGNLAKMAQQMQAEMAKVQAEIETMSVETTAGGGAVTATVNG
                     HGEVLSLTISPDAVSADDVEMLQDLITAAVNDGVRQIKAQIEAKTSRITGGMNLPGML
                     G"
     gene            complement(1620..3293)
                     /gene="dnaX"
                     /locus_tag="VIF08_00970"
     CDS             complement(1620..3293)
                     /gene="dnaX"
                     /locus_tag="VIF08_00970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY8134582.1"
                     /translation="MAADVTAPTPHQALYRRWRSQTFKDVVAQDAIIETLRNAVRLNR
                     LAHGLLFVGPRGTGKTSTARIVAKAVNCPNVVDGEPDDTCESCVAIRDGRALDVVELD
                     AASNNRVDDMRELLPRVYTAPADLKHKVFIIDEVQRIKEGWDVLLKTLEEPPPDVLFI
                     FCTTDPSGIRPAVVSRLQRFTFRPLPEKQISAKLRRIAEAEGRTITDEAIGLIAQRAA
                     GGMRDAESMLDQVISSGIESINADTVRDLLGLAEMESVDRFIDDLASGNALDGIRLLD
                     DLEADGRDLVAFADQVVARLREQLVSALGNNRLGADRAQRLTRAARRLAGLDVNRGAA
                     GGYRLQLELALLEGGNGATTDRPITAVQQPTTRPAAQPASAQPAPTHAKPRQEPRAVK
                     VEAEAKPVEPVAPLVNSVAPSVESPAPPPESLSPPVGSAAPATAAIGDALAELLTAWP
                     RVVERVSRNPADRPLISACRPVEVSGATIVLGFPESQAFMRDIATRKQRKLEEGIGEV
                     LGRAVAVRCVATNIELIPQELTVAGGSDDLVAQARKIFADDLADVAEVD"
     gene            complement(3316..3579)
                     /gene="ffs"
                     /locus_tag="VIF08_00975"
     ncRNA           complement(3316..3579)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIF08_00975"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(3802..3891)
                     /locus_tag="VIF08_00980"
     tRNA            complement(3802..3891)
                     /locus_tag="VIF08_00980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3853..3855),aa:Ser,seq:gga)
BASE COUNT          619 a         1336 c         1304 g          659 t
ORIGIN      
        1 ttattcccca cttcggggta ggtcacctac gcgttactca gccgtccgcc actagcgact
       61 cagagcaagc cctgagtcac ccgttcgact tgcatgtctc atgcacgccg ccagcgttta
      121 tcctgagcca ggatcaaact ctccgacaaa gaatcggcct tgcggtcgat tcagaaactc
      181 ggggtttgcc cgctcgcact agctgattgc ctaccactct tcacttgtta aggtgcagcg
      241 tccagagcct ttccggcgac tggcacggcc gcgcattgtg acacgatcac aagcgggccg
      301 tcaaccgtac ggtgcgggat ctgggggctc agtgactgag cctggccggg ccacctacgc
      361 ggtgaccgcg gaacaggaac tataacacga tcgtgccgcg cattgcacgc gcgcgacggg
      421 tcgtgaggcc tagagcgggc cctcctgggc gagcaggatg aaggccgccc cgctgagcca
      481 cgacagcagc gccagctcga cgctgccggg cgctcccccg ggacccgtcg cgccccagta
      541 gacgagcccg cccagcaaca ctgccagcag cggcttgacc agccgcagca gcatctcgat
      601 gacgaggttg ctcaccgtcg caccatagat gttcgcgcgc ggctgctcat cgctcgttga
      661 cctcgaacgc gcgccggccc tgcatcgccc ggaccagtgt cacctcatcg gcgtactcga
      721 ggtcgccgcc cacggggatg ccgcgcgcga tccgagtgat ggcgccgacg tcgttggcca
      781 gccgctcggc gaggtacatc gccgtcgcct cgccctcgag cgtgggattg gttgccagca
      841 cgacctcctg gtacggatcg ccatcgcgcg accgatccgc cgcgcgctcg agcagctcgc
      901 gcacgcgcag ccggtccggt ccgatgccgt ccatgggcga gatcgcgccg tgcaggacgt
      961 ggtagcggcc cttgaacgcg ccggtgcgct ccagggcgag cacatcgagc ggctcctcga
     1021 cgacgcacac ccgtgtctgg tcgcgcgacg gatcggcgca gatggggcac tgcggcccgg
     1081 tcgagatgtt gaagcaggtc tcgcagtaca ccacctcgtc gcgcaccgcg acgagcgccg
     1141 acgcgagagc gcgtgcctcc gattccgggg cgcgcaatag gtggtaagtg agccgttgcg
     1201 cggtcttggg ccccacgccg ggcagccgcg acagcgcctc gatcaggcgc gcaacgggtt
     1261 cgatcaacgc cgccatggag ccggctctgc ctagccgagc atgcccggca ggttcatgcc
     1321 gcccgtgatg cgcgacgtct tggcctctat ctgcgccttg atctggcgga cgccgtcgtt
     1381 gaccgccgcg gtgatgaggt cctgcagcat ctcgacgtcg tcggcgctga ctgcgtcagg
     1441 actgattgtc agcgacagga cttcgccatg gccgttgacg gtcgccgtaa ccgcgccgcc
     1501 gccagccgtc gtctcgacgc tcatcgtctc aatctcggcc tgcaccttgg ccatctcggc
     1561 ctgcatctgt tgggccatct tggccaggtt cccgtaaccc actatgcctc cttgggtagt
     1621 cagtcgacct cagccacgtc ggccaggtcg tcggcgaaga tcttgcgcgc ctgggcaacc
     1681 aggtcgtcgc tgccaccggc gaccgtcagc tcctgcggga tcagctcgat gttggtggca
     1741 acgcaacgaa cagcgaccgc gcggcccagt acctcgccga tcccctcttc cagcttgcgc
     1801 tgcttcctcg tggcgatgtc acgcatgaac gcttggcttt ccgggaagcc cagcacgatc
     1861 gtcgcgccac tgacctcgac cgggcggcag gcgctgatca gcggccgatc ggcggggttg
     1921 cggctgacgc gctcgacgac gcgcggccac gcggtaagga gctcggcgag agcgtcgccg
     1981 atcgccgcgg tcgcaggggc cgccgatccg acaggaggtg acaacgattc gggagggggt
     2041 gccggcgatt cgaccgaagg tgcgaccgaa ttgaccagag gcgcaaccgg ctccacgggc
     2101 ttggcctccg cctcaacctt tacggcgcgc ggttcctgcc tgggcttcgc gtgggttgga
     2161 gcaggctggg ccgatgcggg ctgggcagcg ggccgggtgg tcggctgctg aacagcggta
     2221 atcggtcggt cggtcgtagc tccgttccca ccttccaaca gggcgagctc gagctgcagc
     2281 cgatagccgc cggcagcgcc gcggttcacg tccagcccgg cgagccggcg cgcggcgcgg
     2341 gtcaggcgct gcgcacgatc ggcgcccagg cggttgttgc cgagtgcgct gaccagttgt
     2401 tcgcgcaggc gcgccacgac ctggtcggcg aacgcgacga gatcgcgacc gtcagcctcc
     2461 aggtcgtcga gcagccgaat gccgtcgagg gcgttgcccg acgcgaggtc atcgatgaag
     2521 cgatcgacgc tctccatctc cgccaggccc aacaggtcgc gcaccgtgtc ggcgttgatc
     2581 gattcgatgc cgctcgagat gacctggtcg agcatcgact ccgcgtcgcg catgccgccc
     2641 gcggcgcgtt gcgcgatcag gccgattgcc tcgtcggtga tggtgcgccc ttccgcctcc
     2701 gcgatgcggc gcagcttggc gctgatctgc ttctcgggca gcggccggaa cgtgaagcgt
     2761 tggagccggc ttacgacggc ggggcggatg cccgacggat cggtcgtgca gaagatgaag
     2821 aggacgtcgg gcggtggctc ctcgagcgtc ttcaggagca cgtcccagcc ttccttgatg
     2881 cgctggacct cgtcgatgat gaagaccttg tgcttcagat cggcgggggc ggtgtagacg
     2941 cgcggcaaca gctcgcgcat gtcgtcgacg cggttgttgc tcgcggcgtc gagctccacc
     3001 acatcaaggg cgcggccgtc gcggatcgcg acacacgact cacacgtgtc gtcgggctcg
     3061 ccgtcgacga cgttgggaca gttgaccgcc ttggccacga tccgcgcggt gctcgtcttg
     3121 ccggtgccgc gcggcccgac aaagagcaga ccgtgcgcca ggcggttcag ccgcacggcg
     3181 ttgcgcagcg tctcgatgat cgcgtcctgg gctacgacgt ccttgaacgt ctgcgaccgc
     3241 cagcgccgat acagcgcctg atggggagtc ggggcagtca cgtccgcagc cacgaacgca
     3301 gtgtagggaa gtttgccgtg cacccgccgt cgaccgggac gctcgcgtgc ccaacgaaga
     3361 gatcggctca gaccaggcct gtccgcggca cccggcgagc agcgtttatc gctgcttcct
     3421 tccggacctg acgaggttca cgcgtcgccg ctgcgcggga cccgatcctc aacaccaatc
     3481 ttcccgccgg cgttcgcgaa cgcgggcctt gggtgggaat tcagccctgc tgaagcggat
     3541 tgcaggtaca gggcaccgct agttccccgc ctagcacggc accgcgattc tactaggacg
     3601 cgtcgcgtgc gcccagcgca cccacggtcc gccgaacgac atccaccgag ccttccgccg
     3661 cgttctggtg cggacccggc accgagtgac ggctggaggt gacgattcga agtcgaagcc
     3721 agtcttctcg aattcgaagc cagtcttcga cggacggtag gtgcgttgag cgcaccactg
     3781 cgacccaaag agcaagggag ttggcggaga gggagggatt cgaaccctcg atgggtttcc
     3841 ccatacggca tttccagtgc cgcgcactag gccaaactat gcgacctctc cgcgggcgta
     3901 cgtgacttag atcttggg
//
LOCUS       DATBRX010000027         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_7410, whole genome shotgun sequence.
ACCESSION   DATBRX010000027 DATBRX010000000
VERSION     DATBRX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7410"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1905
                     /locus_tag="VIF08_00985"
     CDS             <1..1905
                     /locus_tag="VIF08_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8134583.1"
                     /translation="PWQKLKRSLLLLLQLLLALLLAFVVARPFSEHPAGLARDLVLVI
                     DASASMSATDVFPDRLTAAKRLAQEQLAQVPSDGRVSVIAAGDTARVVANEATDRGRI
                     SQAIESVEASTSASDLTDALKLANSLAKRARGAEVLVVTDDAFSASPDVLLEVPVRVL
                     TVGRERDNQAIAALAVRADPSGLKRTLFVSVANYSGSIVGRSLQILADGVPVTARDLF
                     LDPLTRADVVIDELPAGTSVVEARLAPASATDTPTGPPDYMPLDDAAWAIVPPDRLRR
                     VLLVGPGNVYIQNAFALLPNVELYGATAEQWPTTTGKDKFDLVVFDGFVPADLPNKPI
                     LAFAPPQTSPLGVVLGTLEEFGMGQPAVNDPLLRGVDLTRLHIAKTQQMELPAWARAV
                     LPGSDGPLMYAGVRDAQPTAVFAFDIRESDLPLQVAWPIMVSNLAGELLGLNSAALDP
                     LAPSSPIDIPITPGSQGVRVTLPDGTVETLPPGATGASSVTFVTTRQLGVYRAEVIPA
                     PAPSGSPGASPGATPTPSATPDPSASPGGPVGTEDEPLLFAVDLFSSDESNIRPGDGE
                     RLAALGTEVPADPANVGTARDEFWPLLAALTLLFLVIEWLVYERDGARRVLSGLRGLN
                     PLRRSAGKPA"
     gene            1902..>3289
                     /locus_tag="VIF08_00990"
     CDS             1902..>3289
                     /locus_tag="VIF08_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8134584.1"
                     /translation="MSLPFSVSRPELLIVGLLLFAITLGLSLAARHHLSKGRRRASLI
                     IRSVMLASLVLALSGFAIVWPVDRLTTVYVVDLSDSVGNAGRDSALAFVRDSLVERKG
                     NDQAAIVAFGGEALVERLPAELTDLDQFASVPATSATDIGGALRLAAALFPDETQKRI
                     VLVSDGNDTTGRGQSEAALAAARGVQIQTYVVGLGAADEVIVQGVRSPATARVGEDIE
                     VEVTISSTVAQPASVRLFGDGTQIGQQSVNLVPGINRVTFTTSATEAGFHTFRAVVEA
                     EHDTFAANNRGDSNTIVKGDPRILIIAGEPEAAANLSAALVAENQDVDVLAPEQAPTD
                     LTGFASYDAVVLADVPQTRLGLNRMIALQVFTRDLGRGLVMIGGPNSYGAGGYKRTPL
                     EEALPVDMDVRDRQRQPDIALVVVIDKSGSMDACHCNTSNRDNGVQINGVPKVDIGKE
                     AILRAVSALTER"
BASE COUNT          473 a         1212 c         1089 g          515 t
ORIGIN      
        1 ccgtggcaga agctcaagcg ctcgctgctt ctcctgctgc agctgctgct cgcgctgctg
       61 ctcgcgttcg tcgtcgcgcg gccgttcagc gagcatcccg cgggccttgc ccgtgacctc
      121 gtcctggtca tcgacgcgtc ggcatcgatg tccgccacgg acgtcttccc cgaccgactg
      181 accgccgcca agcgcctcgc ccaggaacag ctggcccagg tcccatcgga cggccgggtg
      241 agcgtgatcg ccgcaggcga taccgcccgc gtggtggcca acgaggcgac ggatcggggc
      301 cgcatcagcc aggccatcga gtcggtcgag gcgtcgacat ctgcgtccga tctgaccgac
      361 gcgctcaagc tggccaactc gctcgccaag cgggcacgtg gtgcggaggt gctcgtcgtg
      421 accgacgacg cgttctcggc ctcgccggac gtcctcctcg aggtgccggt cagggtcctg
      481 accgtgggcc gtgagcgcga caaccaggcc atcgccgcgc tggcggtccg cgccgatcca
      541 tccggcttga agcgcacgct cttcgtgagc gtcgccaact actcgggctc catcgtgggc
      601 cgctcgctgc agatcctggc cgacggcgtg ccggtgacgg cgcgcgacct gttcctcgac
      661 cccctcaccc gcgccgacgt ggtgatcgat gagctgcccg ccggcacgtc ggtcgtggag
      721 gcgcgcctcg cgcccgccag cgcaactgac acgcccaccg gcccgccgga ctacatgccg
      781 ctcgatgacg ccgcatgggc gatcgtgccg cccgaccgcc tgcgccgcgt cctgctcgtg
      841 gggccgggca acgtctacat ccagaacgcg ttcgcgctgc tgcccaacgt cgagctgtac
      901 ggggccacgg ccgagcaatg gccgaccacc acgggcaagg acaagttcga cctcgtcgtc
      961 tttgacggct tcgtgccggc cgacctgccg aacaagccca tcctcgcctt tgctccgcca
     1021 cagaccagcc cactcggcgt cgtcctgggg acactcgagg agttcggcat gggccagccg
     1081 gcggtcaacg atccgctgct gcgcggagtc gatttgacgc ggctgcacat tgccaagacc
     1141 cagcagatgg agctgcccgc gtgggcacgc gccgtgctgc ccggttccga cggcccgctg
     1201 atgtacgccg gcgtgcgcga cgcccagccg acggccgtct tcgctttcga catccgcgag
     1261 tccgacctgc cgctccaggt ggcgtggccg atcatggtgt ccaacctcgc gggtgagctg
     1321 ctgggcctga acagcgcggc gctcgacccg ctcgcgccgt catcgcccat cgacatcccg
     1381 atcacgcccg gttcgcaggg cgtgcgcgtc accttgcctg atggcacggt cgagactctg
     1441 ccacccggcg ccacaggcgc atcgagcgtc accttcgtca ccacgcgcca gctgggcgtt
     1501 taccgggcgg aggtcatccc ggcgcccgcg cctagcggct cacccggcgc gtcgcccggc
     1561 gcgacgccca ctccgtcggc gacgcctgat ccctccgcca gcccaggcgg accggtgggc
     1621 acagaggacg agccactgct cttcgcggtc gatctcttca gcagcgacga gtcgaacatc
     1681 cgcccgggcg acggcgagcg cctggccgca ctgggaacgg aagtgccggc ggatccggcg
     1741 aacgtgggca ctgcgcgcga cgagttctgg ccgctgctgg cagcgctgac gctgctgttc
     1801 ctcgtcatcg agtggctcgt gtatgagcgc gacggcgcgc gtcgcgtgct gagcggcctg
     1861 cgtggcctca atcccttgcg ccgctcagcg ggcaaaccgg catgagcctg ccgttcagcg
     1921 tcagccgccc ggagctgctg atcgtcggcc tgctgctctt tgccatcacg ctcggtctgt
     1981 cattggccgc gcgccatcac ctgtccaagg gccggcgacg cgcgtcgctg atcatccgct
     2041 cggtcatgct cgccagcctc gtactggcgt tgtctggctt cgccatcgtt tggccggtcg
     2101 atcgcctgac caccgtctac gtggtggatc tgtccgattc cgtcggcaac gccggccgtg
     2161 actcggccct cgccttcgtc agggattcgc tcgtcgagcg caagggcaac gaccaggcgg
     2221 ccatcgtcgc gttcggcggc gaggcgctcg tcgagcggct gcccgcggag ctgaccgatc
     2281 tcgaccagtt cgcctcagtg ccggcgacgt ccgcgaccga catcggcggc gcgctgcgac
     2341 ttgcggccgc gctattcccg gacgagacac agaagcgtat cgtgctcgtc agcgacggca
     2401 atgacacgac cggccgcggc cagagtgagg ccgcgcttgc cgccgcgcgc ggtgtccaga
     2461 tccagaccta tgtcgtgggc cttggcgcgg ccgatgaggt gatcgtccag ggcgtgcgct
     2521 cgcccgccac cgcgcgcgtc ggcgaggaca tcgaggtcga ggtgacgatc tcgtcgaccg
     2581 tggcccagcc cgcgtcagtc cggctgttcg gcgacggcac gcagatcggc cagcagtcgg
     2641 tcaacctcgt gcccggcatc aaccgcgtga ccttcacgac gagcgccacg gaagcgggct
     2701 tccacacctt ccgtgccgtg gtcgaagccg agcacgacac gttcgccgcc aacaatcgcg
     2761 gcgactcgaa cacgattgtc aagggcgacc cgcgcatcct gatcatcgcc ggcgagcccg
     2821 aggccgcggc caacctgagt gccgcgctcg tggcggagaa ccaggacgtc gatgtcctcg
     2881 cgcccgagca ggcgccgacc gatctgaccg gcttcgcctc gtacgacgcg gtcgtgctcg
     2941 ccgacgtgcc ccagacgcgc cttggcctga accggatgat cgccctgcag gtgttcacgc
     3001 gcgacctggg ccgtggcctg gtgatgatcg gcggccccaa ctcctatggc gcgggtggct
     3061 acaagcgcac gccactcgaa gaggcgctgc ccgtcgacat ggacgtgcgc gaccgccagc
     3121 gccagccgga catcgcgctc gtggtcgtaa tcgacaagtc gggctccatg gacgcgtgcc
     3181 actgcaacac gtcgaatcgg gacaacggcg tccagatcaa cggcgtgccc aaggtggaca
     3241 tcggcaagga agccatcctg cgcgcggtca gcgccctgac ggagcgcga
//
LOCUS       DATBRX010000028         5808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2912322, whole genome shotgun sequence.
ACCESSION   DATBRX010000028 DATBRX010000000
VERSION     DATBRX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2912322"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VIF08_00995"
     CDS             <1..419
                     /locus_tag="VIF08_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134585.1"
                     /translation="NITVNAAKIVATLGQGPVGAINVTYNAADGRLDLAGASSATLNG
                     TLNASTIGLTLPSGSFNGNITINASTLNVCSAADTGLRITYDDTLSSQNFASMGLTQS
                     GKTWQSANYSTARAHAEIHISANVSTTNLNPAGGCQ"
     gene            416..928
                     /locus_tag="VIF08_01000"
     CDS             416..928
                     /locus_tag="VIF08_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY8134586.1"
                     /translation="MNRRLFRSPDDRVLAGVAGGMAETYDLDPALVRVGWALLILVSG
                     GIFLLLYIVMALVVPLRPAGIGWAAAMAGGPSAPMGAGGAPDPSSGGAMPPAGPQSGS
                     PDWSYRRTRHHDGNGALVIGLILIVAGAFFLARQFIPALNWSLVWPLIAIAGGLALVL
                     LAMSRGRSDS"
     gene            925..1731
                     /locus_tag="VIF08_01005"
     CDS             925..1731
                     /locus_tag="VIF08_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8134587.1"
                     /translation="MSRSDPAAPATRQDRRKLELEQRREQRRVARERPAPSPLRSPMA
                     MFTGAALLVGVLIVAFMWFTRPAAPTVNDLAPPADEIPAGVVIDGRTMGSAAAKVTVE
                     IWSDFQCPACRAFATDLEPRTITDYVVTGKVRLVYRDAAFQGQKSSSPYDESVEPAAA
                     ARCAADQGLFWQMHNWLFANWNGENEGAFAAPRLSAIATAAGLDLATYDSCMAAGDKQ
                     TAVTNETNQGVAAGISSTPTLIINGQALVGAPRAYADWASILDQAYAAAN"
     gene            1742..2224
                     /locus_tag="VIF08_01010"
     CDS             1742..2224
                     /locus_tag="VIF08_01010"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY8134588.1"
                     /translation="MITRRVAARPLLGVHPAIILAVLDVIGLLVAGYLSVVELSGGVP
                     TCGIISGCEEVAKSQYNNFLGIPVAVFGVLLSVVLLSLALAWWKTDIYGLLLGHYALS
                     LVGVFFDGWFLYVQVFVIEAVCIWCVTYEVSLLLRFLIALVVYLRQPRPGDADPPAAD
                     "
     gene            2235..4628
                     /locus_tag="VIF08_01015"
     CDS             2235..4628
                     /locus_tag="VIF08_01015"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEY8134589.1"
                     /translation="MTPISEHDVELVDAYWRAANYLSVGQIYLFDNPLLRRPLTRDDI
                     KPRLLGHWGTTPGLNFIWAHADRQIKARDLDSIMIVGPGHGGPAAVANAYLEGTWSER
                     YSSAEMDEDGMRRLFRSFSYPGGIGSHVTPEVPGSIHEGGELGYSLAHAYGAAFDNPD
                     LVVFCVVGDGEAETGPLAGSWHSNKFLSPVSDGAVLPILHLNGYKIANPSFLARVPEA
                     ELRALFEGYGYRPLFVSGDEPAAMHREFAAALDDALDDIAAIQERARSGRTSRSGGAR
                     RTARAGHSAKRAAWPMIVLRTPKGWTGPKVVDGKPVEGTWRSHQVPLDAVRDKPEHLA
                     QLEAWLRSYRPDELFDRAGRLIKALTDFPPTGDRRMSANPHANGGELLRDLEMPDFRD
                     YAVEVKRPAREFGEATRALGRFLRDVIEANPTNFRLFGPDETDSNRLSAVFETTDRQF
                     AGELLPTDEHQAPQGRVMEVLSEHLCEGWLEGYLLTGRHGLFNCYEAFIHIVDAMFNQ
                     HAKWLKISSEIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDVVINKKAEVVRVY
                     LPPDANTLLSVADHCLRSRQYVNVIVAGKQAALQYLSMDDAVLHAARGLGIWPWASST
                     TGEPDVVLATAGDVPTLEALAAADLLRRHLPDLGVRFVNVVDLMRLQPESEHPHGLPD
                     REFDALFTTDKPIIFAFHGYPWLIHRLTYRRKNHANLHVRGYKEEGTVTTPFDMALRN
                     DIDRYHLVMDVIDRVPGLGERAADVRQQMVDARLTAQMHAREHGADADEIAKWTWPH"
     gene            4610..5779
                     /locus_tag="VIF08_01020"
     CDS             4610..5779
                     /locus_tag="VIF08_01020"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY8134590.1"
                     /translation="MDVAALSGRRVLVLNAGSSSLKASVLEIDERGRVANDGRPLAAT
                     QTEWGSDATRVADRRATVERALNSLWAGGVGRDSITVAGHRVVHGGTRFRGATLIDDA
                     ALTELEALGDLAPLHNAVAIDTLRAQRDLMPDVLAVAAFDTAFHATLTEQAYVYPLPW
                     AWYEDWGIRRFGFHGLSVAWSVRRAAELLARSPDELCLVVVHLGSGCSVTAVQSGRSI
                     STSMGLTPLEGLTMGTRSGSVDPGILLHVMRQRGLSADRLAEVLDHESGLLGISGVAG
                     DMRAVSDAAAGGNARARLAIDIFVRRAAAGIAVAATSLLRLDAVVFTGGIGEHAADVR
                     AQIVARLGPLQLEPLGEPVGSGDGLLSAPEAGIAVLRVAAREDIVIAEAAATVAH"
BASE COUNT          883 a         2041 c         1945 g          939 t
ORIGIN      
        1 gcaacatcac ggtcaacgcg gccaagatcg tggccactct cggacagggg ccggtcggcg
       61 cgatcaacgt aacctacaac gcggctgacg ggcgcctgga ccttgccggt gcatccagcg
      121 cgacgctcaa cggaacgctc aacgcatcga ccatcgggct gaccctgccg agtggctcat
      181 tcaacggcaa catcacgatc aacgcctcga cgctcaacgt atgctcggcc gccgataccg
      241 ggctgcgcat cacctacgac gacacactgt catcgcagaa ctttgcgagc atgggactga
      301 cgcagtcagg caagacctgg cagtcggcca actactcgac cgcaagggca cacgccgaga
      361 ttcacatctc agccaacgtc tcgacgacca acctgaatcc cgcaggaggc tgccaatgaa
      421 ccgccggcta tttcgctcac cggacgatcg cgtccttgcc ggtgtggccg gcgggatggc
      481 ggagacctac gacctcgacc cagcactggt gcgcgttggc tgggcgctgc tgatcctcgt
      541 cagcggcggc atcttcctgc tgctgtacat cgtcatggcc ctggtcgtgc cgctccggcc
      601 ggccggcatt ggctgggcgg ccgcgatggc cggcgggcct tccgccccga tgggtgcggg
      661 cggcgcgcct gatcccagca gcggtggtgc catgccgcct gccggtccgc agagcggctc
      721 gcccgactgg tcctaccgca ggacccggca ccacgacggc aacggcgccc tcgtcatcgg
      781 gctcatcctg atcgtcgccg gcgccttctt cctggcgcgc caattcatcc cggcactgaa
      841 ctggagcctc gtctggccac tgatcgcgat cgctggcggc ctggccctcg tcctgctcgc
      901 catgagccgc ggcaggtcgg attcgtgagc cgttccgacc cggccgcacc ggccacccgc
      961 caggaccgac gcaagctcga gctcgagcag cggcgtgaac agcgccgcgt cgcgcgcgag
     1021 aggcccgcgc cttctccgct gcgttcgccc atggcgatgt tcaccggggc ggcgctgctg
     1081 gtcggcgtcc tgatcgtggc cttcatgtgg ttcactcggc cggccgcgcc gacagtcaat
     1141 gatctcgcgc cgccagccga cgagattccg gccggcgtgg tcatcgacgg acgcacgatg
     1201 ggcagtgccg ccgccaaggt cacggtcgag atctggtcgg acttccagtg cccggcatgc
     1261 cgggcgttcg cgaccgacct cgagccacgg acgatcactg attacgtcgt gaccgggaag
     1321 gtcaggctcg tctaccgcga cgccgcgttc cagggccaga agtcgtcctc gccgtacgat
     1381 gagtcggtcg agccggcggc ggccgctcgc tgtgccgcgg atcaggggct tttctggcag
     1441 atgcacaact ggctgttcgc gaactggaat ggcgagaacg agggcgcgtt cgcagcgccg
     1501 cggctgagcg ccatcgccac ggcggccggg ctcgacctgg ccacgtacga ttcgtgtatg
     1561 gccgccggtg acaagcagac cgcggtgacc aacgagacga accagggtgt cgcggctggc
     1621 atttcctcga cacccacgct gatcatcaac ggccaggcgc tggtcggcgc accgcgcgcc
     1681 tacgctgact gggcgtcaat cctcgaccag gcctacgccg cggccaactg agggcagggc
     1741 cttgatcact cggcgcgtcg cggcgcggcc actgctgggc gtccatcccg cgatcatcct
     1801 ggcggtcctg gatgtcatcg gcctgctcgt cgcgggctac ctgtcggtcg tcgagctgtc
     1861 gggcggcgtg ccgacgtgcg gcatcatcag cggctgcgaa gaggtcgcca agagccagta
     1921 caacaacttc ctgggcatac cggtggcggt gttcggagtg ctgctgtcgg tcgtcctgct
     1981 gagcctggcg ctggcttggt ggaagaccga catctacggc ctgctgctgg gtcactacgc
     2041 cctgtcgctg gtcggagtgt tcttcgatgg ctggttcctg tatgtccagg tcttcgtcat
     2101 cgaggcggtg tgcatctggt gcgtcacgta cgaggtgtcg ctgctcctgc ggttcctgat
     2161 cgcgctggtg gtgtacctgc gccaaccccg tcccggagac gccgacccac ctgctgcgga
     2221 ctaaccttcc cgggatgacg cccatctccg agcacgatgt cgagctcgtc gacgcctact
     2281 ggcgcgcggc gaactacctg tccgtgggcc agatctacct gttcgacaac cctctgctgc
     2341 gtcgcccgtt gacgcgggac gacatcaaac cacgcctgct cggccattgg ggcacgacgc
     2401 ccgggctgaa cttcatctgg gcccacgccg atcgccagat caaggcgcgc gatctcgact
     2461 cgatcatgat cgtcggcccg ggccacggcg ggccggcagc cgtggccaat gcctatctcg
     2521 agggcacttg gtccgagcgc tattcctcgg cggagatgga cgaagacggc atgcgcaggc
     2581 tgttccgctc gttctcgtat ccgggcggca tcggctcgca cgtcacgccc gaggttccgg
     2641 gttcaatcca cgaaggcggc gagctgggct actcgctcgc gcatgcctat ggcgcggcct
     2701 tcgacaaccc cgatctcgtc gtcttttgtg tcgtgggcga tggcgaggcg gagacgggcc
     2761 cgctcgcggg cagctggcac tcgaacaagt tcctgagccc ggtgagtgat ggcgccgtcc
     2821 tgcccatcct gcacctgaac ggctacaaga tcgccaatcc cagctttttg gcgcgcgtcc
     2881 ccgaagccga gctgcgcgcg ctgttcgagg gctacggcta ccggccgctg ttcgtctcgg
     2941 gcgatgagcc ggccgcgatg catcgcgagt tcgcggccgc gctcgacgac gcgctcgacg
     3001 atatcgccgc gatccaggaa cgcgcccgca gcggccgcac cagccgcagc ggcggcgcca
     3061 ggcgcaccgc ccgcgccggg cactccgcca agcgcgccgc ctggccgatg atcgtgctgc
     3121 gcacgcccaa gggctggacc ggcccgaagg tggtcgacgg caagccggtg gaaggcacat
     3181 ggcgctccca ccaggtaccg ctcgacgcgg tgcgtgacaa gcccgagcac ctcgcccagc
     3241 tcgaggcatg gctgcgctcc tatagacccg acgagttgtt cgatcgcgcg ggccggctga
     3301 tcaaggcgct gaccgacttc ccgcccacgg gcgaccgacg catgtcggcc aacccgcatg
     3361 ccaacggcgg cgagctgctg cgcgacctgg agatgcccga cttccgcgac tacgcggtcg
     3421 aggtcaagcg gccagccagg gagttcggcg aagccacgcg cgcgctgggc cgcttcctgc
     3481 gcgacgtgat cgaggccaat ccgaccaact tccgattgtt cgggccggac gagaccgact
     3541 ccaaccggct atcagcggtg ttcgagacga ccgatcgcca gttcgcgggc gagctcctgc
     3601 cgaccgacga gcaccaggcg ccgcagggcc gggtgatgga ggtgctgtcc gagcacctct
     3661 gcgagggctg gctggagggc tatctcctga ccggacggca cggcctgttc aactgctacg
     3721 aggcgttcat ccacatcgtc gatgcgatgt tcaaccagca cgccaagtgg ctgaagatca
     3781 gcagcgagat cccctggcgc cggcccatcg cgtcgctcaa ctacctgctg tcatcgcatg
     3841 tctggcgcca ggatcacaac ggcttcagcc accaggatcc ggggttcata gacgtcgtga
     3901 tcaacaagaa ggcggaggtc gtgcgcgtct acctgccgcc cgacgcgaac acgctcttgt
     3961 cggtcgccga tcactgcctg cgctcgcgcc agtacgtcaa cgtaatcgtc gccggcaaac
     4021 aagctgcgct gcagtacctg tcgatggacg acgcggtgct gcacgccgcg cgcggcctgg
     4081 gcatctggcc gtgggccagc agcacgaccg gcgagccgga cgtcgtcctc gcgaccgcgg
     4141 gcgatgtgcc cacgctggag gcgctggccg cggccgatct gctgcgccgc catctgcctg
     4201 atcttggggt gcgcttcgtc aacgtcgtgg acctgatgcg cctccagccc gagtccgagc
     4261 atccgcacgg cctgccggac cgcgagttcg acgcgctgtt cacgaccgac aagccaatca
     4321 tctttgcctt ccacggctac ccctggctca tccatcgcct gacctatcgc cggaagaacc
     4381 acgccaacct ccatgtgcgc ggctacaagg aagagggcac ggtcacgacg ccgttcgaca
     4441 tggcgctgcg caacgacatc gatcgttatc acctggtgat ggacgtgatc gaccgcgtgc
     4501 cggggctggg tgagcgcgcc gccgacgtgc gccagcagat ggtcgacgcg cggctgacgg
     4561 cacagatgca cgcacgcgag cacggcgccg acgcggacga gatcgccaag tggacgtggc
     4621 cgcactgagc ggtcggcgcg tactcgtcct gaacgcgggc tcgagctcgc tcaaggcgag
     4681 cgttctcgag atcgatgagc gcggccgcgt ggccaacgac ggccggccgc tcgccgcgac
     4741 ccagaccgaa tggggttcgg atgcaacgcg cgttgccgac cggcgggcaa ctgtcgagcg
     4801 ggcgctcaac tcgctctggg cgggcggcgt cggccgcgac tcgatcacgg tcgccggtca
     4861 tcgcgtcgtc cacggcggga cgcgctttcg tggggcgacg ctcatcgatg acgccgcgct
     4921 caccgaactc gaggcgctgg gcgatctcgc gcctctgcac aacgcagtgg cgattgacac
     4981 cctgcgcgct cagcgcgacc tcatgcccga tgtcctcgcg gtggccgcct tcgacacggc
     5041 ctttcacgcc accctgaccg agcaggcgta cgtctatccc ctgccctggg cgtggtacga
     5101 ggactggggc atccggcgct tcggcttcca cggcttatcc gtggcctggt cggtccgccg
     5161 cgctgcggag ctgctcgcgc ggtcaccgga cgagctgtgc ctggtggtgg tccacctggg
     5221 cagcggctgc tcggttacag ccgtccagtc gggccggtcg atctcgacgt cgatgggctt
     5281 gacgccgctc gaagggctga ccatgggcac ccgctcgggc tcggtcgacc caggcatcct
     5341 cctgcacgtg atgcgtcagc gcggactgtc ggccgatcgg ctggcggagg tgctcgacca
     5401 cgaatcgggc ctgctcggca tatcgggcgt ggccggcgac atgcgcgcgg tgagcgacgc
     5461 cgcggccggc ggcaacgcgc gcgcccggct ggcgatcgac atcttcgtcc gccgcgcggc
     5521 agccggcatc gccgtcgcgg caaccagcct gctgcggctc gacgcggtgg tgttcacggg
     5581 cgggatcggc gagcacgcgg cggacgtgcg cgcccagatc gtcgcccgcc tggggccgct
     5641 ccagctcgag ccgctcggcg aaccggtggg gagcggcgac ggcctgcttt cagcgcccga
     5701 ggctggtatt gccgtgctgc gcgtcgcggc gcgcgaggac atcgtcatag ccgaggctgc
     5761 cgcgaccgtc gcccactgag gcgctccccg ctcagcgcgg acccgaat
//
LOCUS       DATBRX010000029         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1795098, whole genome shotgun sequence.
ACCESSION   DATBRX010000029 DATBRX010000000
VERSION     DATBRX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795098"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VIF08_01025"
     CDS             <1..1040
                     /locus_tag="VIF08_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY8134591.1"
                     /translation="TGADELLAKVLPTGRLTLASDPSALRGAPIVIVVIGTPIDEFLN
                     PSMTLFDRVIDQVAPHISDGALVVMRSTVYPGTTERVARMLAERDVKVDVACCPERIA
                     EGHALEELKNLPQIVGADSDAAAARAAELFSRLGAETVRTTTREAELAKLFTNAWRYM
                     KFAIANQFLMIAHQAGADYNAILHAIRHDYPRAVDLPGPGFAAGPCLLKDTMQLASFT
                     SDHFPMGQSAMQINEGLPAYIVDAMSHRYGGLAGKRVGILGMAFKAESDDTRASLSYK
                     LRKLLQWAGADVLCTDPYVVDERLVGLDEVLANSDILVLGAPHRAYRTLDLDGREVVD
                     VWGARQAGINL"
     gene            1051..2088
                     /locus_tag="VIF08_01030"
     CDS             1051..2088
                     /locus_tag="VIF08_01030"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8134592.1"
                     /translation="MKVLITGAAGFICGYLIEELLAAGHEVVGIDNFSKYGPVSRAYD
                     RHPGYRFVEGDAKDVTLLTALAADCDQVVAAAAMIGGISYFHEFAYDLIAENERILAA
                     TFDAAIAAQRAGGAGGAGGLKRIVVLSSSMVYESTDVYPTPEGAQLTSPPPRSTYGFQ
                     KLASEYFAKGAHEQYGLPYTILRPFNCVGIGERRALRDTDVMSGNVKLALSHVVPDLV
                     VKVVRGQDPLHILGSGEQVRHYTYGADLARGIRLAMESDAAINEDFNLSTAQSTTVRE
                     LAELIWRRINGDRPLSLVHDEPFEHDVQRRVPDVQKAKRVLGFEATTTLEEMLDEVIP
                     WIRAELGEGRI"
     gene            2133..2744
                     /locus_tag="VIF08_01035"
     CDS             2133..2744
                     /locus_tag="VIF08_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8134593.1"
                     /translation="MAAGRSAMWREIGRHLQRYIPADAAVLDLAAGSGNFIANISAAE
                     RWASDLRDTSAEMPPDVRFVCADGLALREHAPLGHFDRVFMSNYLEHLRSPEAVVEQL
                     AVVRDLLKVGGRVIILQPNVRLTGGHYWDFIDHHTPLTERSLEEAALTAGLEPVKSIV
                     RFLPYTTKSRLPQAAWLVRLYLMFPPAWRIMGKQTLFIAQRMK"
     gene            2741..3499
                     /locus_tag="VIF08_01040"
     CDS             2741..3499
                     /locus_tag="VIF08_01040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8134594.1"
                     /translation="MTDSPEAVKAPELTIVVPVYNEPDNIGPTLRRLAAAVHVPAETL
                     VVYDFDADTTVPVVRAMAAELPNVRLLRNELGRGVLNAMKAGIAAARGDYVLITMADG
                     SDEVELVDGMVALARGGADVVAASRYMKGGRQEGGPLLKRTLSRLAGLSLHWVGRLPI
                     HDATNNFKLYRRAFLEGVTIESKGGFELAIELSVKADLAGRRLAEVPTTWRDRTAGQS
                     RFKLRAWLPLYLRWYMHLFRGRLRRASGPRRSAT"
     gene            3496..>3839
                     /locus_tag="VIF08_01045"
     CDS             3496..>3839
                     /locus_tag="VIF08_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134595.1"
                     /translation="MTPERSLFHEPTTLERARAALERVVDVLSRPWVVWAAFLLVVAG
                     VIWFHLPSLHQPLLEHHSFRQTQTAWTALIFHEQGIDLLHPKIPVFGPPWDVPFEFPL
                     FQALGALVMNAGV"
BASE COUNT          608 a         1336 c         1295 g          600 t
ORIGIN      
        1 agaccggtgc ggacgagctg ttggccaagg tcttgccgac cggacgcctg accttggcga
       61 gcgacccgtc agccttgcgc ggcgcgccca tcgtgattgt cgtcatcggc acgcccatcg
      121 acgagttcct caatccgtcg atgacactgt tcgaccgggt catcgaccag gtggcaccgc
      181 acatcagcga tggcgcgctg gtcgtgatgc gctcgacggt ttacccgggc acgaccgagc
      241 gggtggcacg gatgctggcc gaacgtgacg tgaaggtgga tgtggcgtgc tgcccggagc
      301 gcatcgcgga aggccacgcg ctagaggagc tgaagaacct gccgcagatc gtgggcgcgg
      361 acagcgacgc tgccgcggcg cgggccgccg agctcttctc gcgcctgggc gcggagacag
      421 tccgcaccac gacgcgcgag gccgagctcg ccaagctctt caccaacgca tggcgctaca
      481 tgaagttcgc gatcgcgaac cagttcctga tgatcgccca ccaggccggc gcggactaca
      541 acgcgatcct gcatgccatc cggcacgact acccccgcgc cgtcgacctg cccggcccgg
      601 gcttcgccgc aggaccgtgc ctgctgaagg acacgatgca gctcgcgtcg ttcacgtcgg
      661 accacttccc catgggccag tcggcgatgc agatcaacga gggcctgccg gcctacatcg
      721 tcgacgccat gagccaccgt tatggcggcc tcgccggcaa gagggtgggc atcctgggca
      781 tggccttcaa ggctgaatcg gacgacaccc gcgcctcgct cagctataag ctgcgcaagc
      841 tgctgcagtg ggccggcgcc gacgtgctgt gtaccgatcc gtacgtggtc gacgagcgcc
      901 tggtcggcct cgacgaggtc ctggccaaca gcgacatcct ggttctcggg gcgccgcacc
      961 gagcctaccg cacgctcgat cttgacggcc gcgaggtcgt cgacgtgtgg ggcgcgcggc
     1021 aggcggggat caacctctga gggactgcgc ttgaaggttc tcatcacggg cgccgcgggc
     1081 ttcatctgcg gctacctgat cgaagagctg ctggccgctg ggcacgaggt cgtcggcatc
     1141 gacaacttct cgaagtacgg cccggtcagc cgcgcctacg accgccaccc cggctaccgc
     1201 ttcgtcgagg gcgacgcgaa ggacgtgacg ctgctcacgg cactggccgc ggactgcgac
     1261 caggtcgtcg cggcggcggc aatgatcggc ggcatcagct acttccatga gttcgcctac
     1321 gacctgatcg cggagaatga gcgcatcctc gccgcgacct tcgatgccgc gatcgccgcc
     1381 caacgcgcgg gtggcgcggg tggcgcgggt gggctgaagc ggatcgtggt gctgtcgagc
     1441 tcgatggtct atgaatcgac cgacgtctac ccgacgccgg agggcgcgca gctgacttcg
     1501 ccgccacccc gctccaccta cggcttccag aagctggcga gcgagtactt cgccaagggc
     1561 gcgcatgagc agtacggcct gccgtacacg atcctgcggc cgttcaactg cgtcggcatc
     1621 ggcgagcggc gggcgctgcg cgacaccgac gtcatgagcg gcaacgtcaa gctcgcgctg
     1681 tcgcacgtgg tgccggatct cgtcgtgaag gtggtgcgcg gccaggaccc gctgcacatc
     1741 cttggctcgg gcgagcaggt gcgccattac acctacggcg ccgacctcgc ccgcggcatc
     1801 cgcctcgcga tggaatcgga cgccgcgatc aacgaggact tcaacctctc gacggcgcag
     1861 agcaccaccg tgcgcgagct ggccgagctg atctggcgca ggatcaacgg cgatcgaccc
     1921 ttgagtctcg tccacgacga gcccttcgag cacgacgtcc agcgccgcgt gccggacgtt
     1981 cagaaggcca agcgcgtcct cgggttcgag gccaccacga ccctcgaaga gatgctcgac
     2041 gaggtgatcc cctggatccg cgccgagctc ggggaagggc gcatctagcc atcgaccacg
     2101 acgccctcta ccggcaccgg ttcggcccga aggtggcagc gggccggtcg gccatgtggc
     2161 gcgagatcgg gcgccacctc cagcgctaca tcccggccga tgcggcggtg ctcgacctcg
     2221 ccgctggcag cggcaacttc atcgccaaca tcagcgcggc cgagcgctgg gcaagcgacc
     2281 tgcgcgacac atccgcggag atgccgcccg atgtcaggtt cgtctgcgcc gacggcctgg
     2341 ccttgcgcga gcacgcccct ttgggccact tcgaccgggt tttcatgagc aactacctcg
     2401 agcaccttcg ttcgcccgag gcagtggtgg aacagctggc ggtggtgcgc gaccttctga
     2461 aggtcggcgg ccgggtgatc attctccagc cgaacgtccg gctcaccggc ggtcactact
     2521 gggacttcat cgaccaccac acgccgttga ccgagcgcag cctcgaggag gcggcgctca
     2581 ccgccggcct cgagccggtc aagtcgatcg tccgcttcct gccgtacacg accaagagcc
     2641 gtctgcccca ggcggcgtgg ctggtgcgcc tgtacctgat gttcccgccg gcgtggcgga
     2701 tcatgggcaa gcagacactc ttcatcgccc agcgcatgaa atgaccgatt caccagaggc
     2761 agtcaaagca cccgagctga cgatcgtcgt cccggtctac aacgagccgg acaacatcgg
     2821 cccgacgctg cgccggctcg cggccgccgt ccatgtgccg gcggagacac tcgtggtgta
     2881 cgacttcgac gccgacacga ccgtgcccgt cgtgcgcgcc atggcggccg agctgccgaa
     2941 cgtgcgtctg ctgcgcaacg aactggggcg cggagtgctc aacgcgatga aggccggcat
     3001 cgccgccgcg cgcggtgact acgtgctgat caccatggcc gacggatcag acgaggtcga
     3061 gctggtcgat ggcatggtcg cgcttgcgcg cggcggcgcg gacgtggtcg cggccagccg
     3121 ctacatgaag ggtggccggc aggagggcgg cccgctgctc aagcgcaccc tgagccggct
     3181 cgccggcctg tcgctgcact gggttggccg cctgcccatc cacgacgcga ccaacaactt
     3241 caagctgtac cggcgcgcgt tcctcgaggg ggtcacgatc gaatcgaagg gcgggttcga
     3301 gctggcgatc gagctgagcg tcaaggctga cctcgccggc cggcggctgg ccgaggtacc
     3361 gacgacgtgg cgcgaccgga cggccggcca gagccgcttc aaactgcgcg cctggctgcc
     3421 actctacctg cgctggtaca tgcacctgtt ccgcggccgc ctgcgccgcg cgagcggtcc
     3481 gcggcggagc gccacgtgac cccagagcgg tcgctcttcc acgagccgac aaccttggag
     3541 cgcgcgcgcg cggcgctgga gcgcgtggtc gacgtgctct cgcggccgtg ggtggtctgg
     3601 gcggcgttcc tgctggtcgt cgccggggtc atctggttcc acctgccgtc gctgcaccag
     3661 ccgctcctcg agcatcactc gttccgccag acccagaccg cctggactgc gctgatcttc
     3721 cacgagcagg ggatcgacct gctccatccc aagatcccgg tgttcgggcc gccgtgggac
     3781 gtgccgttcg agtttccact cttccaggcg cttggggcgc tggtcatgaa tgccggcgt
//
LOCUS       DATBRX010000030        10564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1013653, whole genome shotgun sequence.
ACCESSION   DATBRX010000030 DATBRX010000000
VERSION     DATBRX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10564
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013653"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..779
                     /locus_tag="VIF08_01050"
     CDS             27..779
                     /locus_tag="VIF08_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134596.1"
                     /translation="MPLIHLRAERDDYAQLVLMPGDPGRATMMAGLLDGGMDNVKLVN
                     QHRSLLGYTGTYKSKPVSIQTSGIGTPSFSIVMEELLMLGATRFIRTGTCGGIQHGLK
                     TGDVVVATASVPMDGSTRTYLHGDPFAPAADFDLTRALVDTARAKGIDPHPGVVQSVD
                     VFYNPDSDYAAKMRSRGVIAVEMEASALFYLAMREQGKGQDVRGACILTVSDTLIKDD
                     DAVGADYMSLESLEEATKKMIEIALDAGTSLA"
     gene            complement(788..1411)
                     /locus_tag="VIF08_01055"
     CDS             complement(788..1411)
                     /locus_tag="VIF08_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134597.1"
                     /translation="MIDNQVLELRLAAALGAPMSAQQRAELDARVFDLIARTRPARRS
                     FGFRLTRGTALAVGLTVLLASVFGAWGAMKLTEAPYGMQDAAAYSAEIDAAKAVTPLP
                     PGATWPARLDSSPDPNAIYAVRSGQSLVEFNAACIWFGYWLDESRHGTPTQRADALAG
                     VLAMGSWTTFSDRSINQSIDYFASLLDAARRGDAAPIRQFVVVNCAE"
     gene            complement(1401..1970)
                     /locus_tag="VIF08_01060"
     CDS             complement(1401..1970)
                     /locus_tag="VIF08_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8134598.1"
                     /translation="MEAIPLPAAQANTQEIDSEDVAERARHDSAAFGELYIACRDVVF
                     RYLRARCGNESDALDLTAVTFERAFEAIPRYRTQGGGVLAWLLRIARNAAIDDARRQR
                     RVVGTADDSGRSYAKSPEDEMLSTETHRLVQMAVARLPEDQREAVGLRFGAGLTARQI
                     GLVIGKSEEATQKLISRAIVRLREDLNDR"
     gene            2096..2932
                     /locus_tag="VIF08_01065"
     CDS             2096..2932
                     /locus_tag="VIF08_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8134599.1"
                     /translation="MIPIGLNPTTIGVSSAWWRDTAIAAEQAGFTGVWSWDHFVSRGK
                     KKDPVLECWTTLTAAAAVTSTLKVGSFINNVMNRHPAVLARMLATLWDQSKGRVELGI
                     GVGGFAAEEASYGINFPEPPVRVDILREAVEVIRRLWSGGPVSYEGQHFQLNDAYAYP
                     APEPPPRIIVGGEKPGGARLAAMVGDGWTTNGPDYEHLLPIHLEELARNGRTRSDVVH
                     MVAVSMARDVPLADQPLIADMATFLGEWQERGADEVVVSWVRPNELEPLLDAAARAGL
                     AG"
     gene            3177..3656
                     /locus_tag="VIF08_01070"
     CDS             3177..3656
                     /locus_tag="VIF08_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134600.1"
                     /translation="MTYAVVDLTQPHDACIRCGRPTPLGVALCESHNPAHIKGPSPTQ
                     VHGTILFGVIGGFIALLLLLRLASSGVGPFPSALDAVATRADGGLSVVVSVTNNGTRP
                     AGASCRVVAAGMPEFHDDVFFTEQIPAGATRAFTATVPPLPSGQALPASLTLAVRCN"
     gene            complement(3663..5270)
                     /locus_tag="VIF08_01075"
     CDS             complement(3663..5270)
                     /locus_tag="VIF08_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8134601.1"
                     /translation="MPTRWTAKTWTLVAAVLGSSVVFLDYSVLSVALPAIGREPRLFV
                     DVLEGQNYIQYGYLLTLSAFLVLAGALSDYYGRKRVFVIGLIGFAVTSLLCAIAPNVE
                     FLIVARLFQGASGAVLVPGSLAVLTTNFEGAEQGRAFGVWAASTALAPIVGPFIGGVL
                     VDTLSWRWIFLLNLPLVAVATWAALRYMKESRDDQANGRFDWLGALLVALAVGGLSFG
                     AIYGQQRQWQDPIAFVALGVGTLATVGLPLYFRWARNPLVPLSLFRSRNFSVTNVSTL
                     LIYGALYVLLFFQVLFMQGTLGYSATATGLSQIPGPLFLVLLSTRFGAWAAKAGPRWF
                     MTFGPLLMALAILWLVRIPSNSAPWLFDVADPATYLPSTGYLVDILPSQLVFGLGLSM
                     MVAPLTTALMRSVSERVSGLASAINNAISRVGPQLAGALIFVVISIAFYNSLHALAPS
                     LDVDSPQVRREYQPLNPAPATADQAVVTAVRSASTDAFHLAMLVSAGLLLAGAAVNGA
                     FISNRQALAGAEGGEGSLEETPTPSGA"
     gene            5297..6127
                     /locus_tag="VIF08_01080"
     CDS             5297..6127
                     /locus_tag="VIF08_01080"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8134602.1"
                     /translation="MSRSPRIEQPFARDLKVAVDAARKAGQLQMERYEKLERIVHKSE
                     HDVVTEVDELSEQLIIGAIRDAFPTDSFLAEESGHSAGPAQGGESAADKSVGRVWVID
                     PLDGTINYANGIPLFCVAIGLCVDGRPTLGVVYDPVRDEMFSAITGGGATLDGTPIRL
                     PVKERLVDCVVSLSLPRRGWAKRDHALRRSIRVSRSMGSAALAIAYLGNGRFDAMVQS
                     GGMSAWDVCAPGVIAAEGGARLTTLDGGPWFDINRPTKSIGLLGAAPVHHQTLLELLA
                     "
     gene            6124..6609
                     /locus_tag="VIF08_01085"
     CDS             6124..6609
                     /locus_tag="VIF08_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8134603.1"
                     /translation="MTSIPVAGLNHAVLYVRDLDVAVDFYTRVFGFEVVDRMRNFACF
                     LRAAGGANHHDLGLFAVGPAAPRPPRGSTGLYHLAWEVPRIEDLALAASVLQEAGALG
                     GASDHGVSKSLYGQDPDGNEFEIMWRVPREQWGDLEKEAGIAPLDLEAELRIHGAKAA
                     G"
     gene            6606..7484
                     /locus_tag="VIF08_01090"
     CDS             6606..7484
                     /locus_tag="VIF08_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEY8134604.1"
                     /translation="MTHWPAGWKPYAEVHKGDNTITGTVLVWPKVAGDPGIPGREICV
                     YLPPSLARSLAAGEGGGHGGRRYPVLYFMDGQNVFDAKTSYIGVEWAADEALEKLAED
                     GIEAIAVAVANHGDDRMNEYNPWRSSLKWRGRAHPTGGKGDAYLEWLVGTVKPLIDRS
                     LPTRAERMATGIVGSSMGGLISLYALFAQRAVFGLAGVMSPAVAFSDYRVVALIEEAP
                     LPPTRIHLDMGWREWRGATRDARKVRDALLSRGFELDRDLHYVEERNARHNEAAWARR
                     LPDALRFLLADFREPR"
     gene            complement(7481..8374)
                     /locus_tag="VIF08_01095"
     CDS             complement(7481..8374)
                     /locus_tag="VIF08_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY8134605.1"
                     /translation="MIGIQVHLLDATYELFRAHFAPRPQRFDAAGNPASATIGVLENV
                     LYLLREEGVTHLGCASDHVIESWRNERWPGYKTSFGVAPDLLRQFAPAEEALTTLGVV
                     VWPLVEWEADDGLGTAAARFADAPGVERVVIMTPDKDMGQCVREDGKVVQFDRRQRKL
                     IDAAGVREKFGCPPESIPDFLALVGDSADGYPGIPGWGKVSAGALLTRYGHLEDIPEK
                     VAHWDVNVRGAATLAASLRDHLDLAFLFRELATLVTDVPIVNQSLDDMRWKGAHRAAF
                     ERVAHELAAPDLIGRVPKWVD"
     gene            complement(8371..9330)
                     /locus_tag="VIF08_01100"
     CDS             complement(8371..9330)
                     /locus_tag="VIF08_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8134606.1"
                     /translation="MAKAKAKQERPTPPKKAATKAGDRRADAQPRPAPAVPGAALGID
                     IGGTGVKAALVDVTTGELLSRRHRVNTPKPSSPDAVIEAVLHVVGLIAAERDLAPDLP
                     VGVGIPGPIKAGKVMTAANIDKRWIGTPAEELLGKALKRRVHAINDADAAGLAEIRFG
                     AGREVAGTVLMLTLGTGIGSGLFVDGQLVPNTEMGHLEWKGRDVELRLSGVAREKRHL
                     RWRAWANEFSAFLARLEILFWPDLIILGGGVSKEMKKYDKWLKSRAPIKPARFLNTSG
                     IVGAAIYASERARAESARPVVLLPVATRADRAAAPGVGRARKA"
     gene            9400..10134
                     /locus_tag="VIF08_01105"
     CDS             9400..10134
                     /locus_tag="VIF08_01105"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8134607.1"
                     /translation="MDVFRIGSAVRAIRIKRHWRQADLAAKANVPRSAVSKIERGRGR
                     ELRLDVVLRIVEALGGRLVLQVQWQGGDLDRLLNARHSAFHESVAPAFVELPGWVLAP
                     EVSFSIRGERGVIDILAWHAASRTLLVIELKTEIVDVNELMGTVGRKRRLAMEIGRER
                     GWNAVRVALWVVVAESKTNRRRVANHAMTLRAALPADGRSIAGWLREPDRPVACLSFW
                     SDAHGAHTKSDLATVKRVRLPRQTAA"
BASE COUNT         1750 a         3544 c         3576 g         1694 t
ORIGIN      
        1 agccaccgcg cacgtagact tcagccatgc cactgattca ccttcgcgcc gagcgcgacg
       61 actacgcgca acttgtcctg atgccgggcg atcccggccg cgccacgatg atggccggcc
      121 tcctcgatgg cggcatggac aatgtcaagc tcgtaaacca gcaccgctcg ctgctcggct
      181 acaccggcac ctacaagagc aagccggtca gcatccagac gagcggcatc ggcacgccgt
      241 cgttctcgat cgtcatggaa gagctgctga tgctgggcgc cacgcgcttc atccggacgg
      301 gcacgtgcgg cggcatccag cacggcctga agacgggcga cgtcgtggtc gcgaccgcgt
      361 cggtcccgat ggacggctcg acgcggacgt atctgcacgg cgatcccttc gctccggccg
      421 cggacttcga tctgacgcgc gcgctcgtcg acaccgcgcg cgccaagggc atcgacccgc
      481 atccgggcgt cgtccagagc gtcgatgtct tctacaaccc cgattccgac tatgccgcca
      541 agatgcgcag ccgcggcgtg atcgcggtcg agatggaggc gagcgcgctc ttctatttgg
      601 ccatgcgtga gcagggcaag ggccaggatg tccgcggcgc gtgcatcctc actgtcagcg
      661 acacactgat caaggacgac gatgcagtcg gcgctgacta catgtcgctc gaaagcctcg
      721 aggaggcgac aaagaagatg atcgagatcg cgctggacgc gggcacctcg ctcgcctgag
      781 ccgcagctta ctcggcgcag ttcaccacta cgaattgcct gatgggcgca gcgtcgccac
      841 gccgggcggc gtcgagcaga cttgcgaagt agtcaatcga ctggttgatg gaccggtcgc
      901 tgaacgtagt ccagctgccc atcgccagca cgcccgccag ggcgtcggca cgttgcgtgg
      961 gcgtgccgtg ccgtgactcg tcgagccagt agccgaacca tatgcaggcg gcattgaact
     1021 cgaccaggct ctgcccggac ctgaccgcgt agatggcgtt tggatcgggc gagctgtcca
     1081 ggcgagccgg ccaggtcgct ccaggcggca gtggcgtgac tgcctttgcc gcgtcgatct
     1141 cagcggaata agcggcggcg tcctgcatgc cgtatggcgc ctccgtcagc ttcatcgcgc
     1201 cccacgcccc aaagaccgac gccagcagaa ccgtgaggcc gacggccaat gccgtgccgc
     1261 gcgtcaacct gaagccgaac gaccttcgtg cgggtcgtgt ccgcgcgatg agatcgaaga
     1321 cgcgtgcatc aagctcggca cgctgctgcg cggacatggg cgcgcccaac gcggcggcca
     1381 gacgcagttc gagcacctgg ttatcgatca ttgagatcct cccgcaaccg gacaatcgcc
     1441 cggctgatca gcttctgggt tgcttcctcg ctcttgccga ttaccaatcc gatttgacgt
     1501 gcggtgagtc cagcaccaaa gcggagcccg actgcctcac gctggtcttc cggcaggcga
     1561 gcgactgcca tctgcaccaa tcggtgtgtc tctgtagaca acatctcgtc ctcgggcgac
     1621 ttggcgtacg acctaccgct gtcgtctgcc gtgcccacga ctcggcgctg gcggcgcgca
     1681 tcgtctatcg ccgcattgcg ggcgatacgt agcagccatg ccagcacgcc accaccctgg
     1741 gtccggtaac gagggattgc ttcgaacgcg cgctcaaagg tgaccgccgt cagatcgagc
     1801 gcatcgcttt cgttgccaca ccgagcgcgc aggtaccgaa agaccacgtc ccggcaggcg
     1861 atgtacagct caccaaaggc agcggaatcg tgccgcgccc gctcagccac gtcctccgag
     1921 tcgatctctt gcgtattcgc ctgtgcggcg ggcaggggga tcgcttccat cacctctcct
     1981 gtacgaagtg tccacccaga taacgtgtgg gtctggccaa ccggacatgg ccagttgaaa
     2041 acgcggccaa atggctgcgc tacgctgcga tcgtgaatcg aaacgcgtgg caagaatgat
     2101 cccgatcggg ctcaacccga ccacgatcgg cgtttcgtcg gcctggtggc gtgacaccgc
     2161 gatcgcggcc gagcaggccg gcttcaccgg cgtctggtcg tgggaccact tcgtgagccg
     2221 tggcaagaag aaggatccgg tcctcgaatg ctggacaacg ctcactgcgg cagccgcggt
     2281 aaccagcacg ctcaaggtcg gcagcttcat caacaacgtc atgaaccgcc acccagcggt
     2341 gctcgcgcgc atgctggcga ccctgtggga ccagtccaag ggtcgggtgg agctgggcat
     2401 cggcgtcggt ggcttcgcgg ctgaggaagc gtcctacggc atcaatttcc ccgagccgcc
     2461 ggtacgggtc gacatcctgc gcgaggcggt ggaggtcatc cggcgcctgt ggtcgggagg
     2521 tccggtctca tatgaagggc agcacttcca gctcaacgac gcgtacgcct atccggcgcc
     2581 ggagccgccg ccacggatca tcgttggcgg cgagaagcca ggcggagcgc gtctcgcggc
     2641 catggtcggc gacggttgga ccaccaatgg ccccgactat gagcacctgc tgccgatcca
     2701 cctcgaggag ctggcgcgca acgggcggac gcggtccgac gtcgttcaca tggtcgcagt
     2761 gtcgatggcg cgcgacgtgc ccctggcgga ccagccgctc atcgccgaca tggcgacgtt
     2821 cctcggggag tggcaggaac gcggcgcgga cgaggtcgtc gtgtcgtggg tgcgacccaa
     2881 cgagctggag ccgctgcttg atgcggccgc gcgggcgggt ctcgccggat agcgctgcga
     2941 gccacattca cgaaatcgag gcaaacggcg actgagcggc gcgtcggacc gtgccagacc
     3001 tgcccagagg cgggcaaggg atcggatgag cagtcgcgcg gcaggcgatt cggggtgaac
     3061 tcgactcgcg gctgctcgct tgctggaaat gacgcgcgat ttcgagaatc tggctcacgt
     3121 cgcctgccgg tttgtggcgc ggcgcgggtt tccgtgctgc ggggtaggct ggccgcgtga
     3181 cgtacgccgt cgttgacctg acgcagccgc acgacgcctg catccgctgt ggccgtccca
     3241 ccccgctcgg cgtggcgctg tgtgagagcc acaaccccgc gcacatcaag ggccccagcc
     3301 cgacccaggt ccacggcacg atcctgttcg gcgtcatcgg cggattcatc gcgcttctgc
     3361 tcctgttgcg cctcgcgagc tccggcgtcg gcccatttcc gtcggcgctc gacgcggtcg
     3421 cgacgcgcgc cgatggcggc ctgagcgtcg tggtgtccgt taccaacaac ggcacgcggc
     3481 cggccggcgc gagctgccgc gtcgtggctg cgggcatgcc cgagttccac gacgacgtct
     3541 tcttcaccga gcagattccg gccggcgcca cccgcgcatt caccgcgacc gtgccgcccc
     3601 tgccaagcgg tcaggctctg ccggcgtcgc tcaccctggc ggtgcgctgc aactaggcgc
     3661 cgctaggcgc cactcggcgt cggcgtttct tccagcgacc cttctcctcc ttcagcaccg
     3721 gcgagcgcct ggcggttgct gatgaacgcg ccgttgaccg ccgccccggc gagcagcaat
     3781 ccggccgaga ccagcatcgc gagatgaaac gcgtccgtgg aggcgctgcg caccgcggtg
     3841 accaccgctt gatccgccgt tgcgggcgcc gggttgagcg gctggtactc cctgcgtacc
     3901 tgcggcgaat cgacatcgag gctgggtgcc agggcgtgga gcgagttgta gaacgcgatg
     3961 ctgatgacga cgaagatcag cgcgccggcg agctgtggac ccacgcgcga gatcgcgttg
     4021 ttgatcgccg acgcgaggcc ggatacgcgc tctgagaccg agcgcatcag tgcggtggtg
     4081 agcggcgcaa ccatcatcga cagacccagg ccaaagacca gctgcgaagg caagatgtcg
     4141 accaggtagc cggtgctcgg gaggtacgtg gccgggtcgg cgacgtcgaa gagccagggc
     4201 gcactgttgg aaggaatgcg caccagccac aggatggcca gcgccatgag cagcggcccg
     4261 aaagtcatga accagcgtgg accggccttg gccgcccacg cgccgaatcg tgtcgagagc
     4321 agcaccagga agagcggccc gggaatctgc gacagcccgg tagccgtcgc tgaatagccc
     4381 agcgtgccct gcatgaagag cacctggaag aagagcagca cgtacagcgc cccgtagatg
     4441 agcagggtcg acacgttggt gacgctgaag ttgcgcgacc ggaacaggct gagcggcacg
     4501 agcgggttgc gcgcccagcg gaagtagagc ggcagcccga ccgtcgcgag cgtccccacc
     4561 ccgagcgcga cgaacgcgat gggatcctgc cactgccgtt gctggccata gatcgcgcca
     4621 aagctcaggc cacctaccgc gagcgcgacc agcagcgcgc ccagccagtc gaagcggcca
     4681 ttcgcctggt cgtcgcggct ctccttcatg tagcgcagcg cggcccacgt ggcgaccgcg
     4741 acgagcggca gattcagcag gaatatccag cgccacgaca gcgtgtcgac gagcacgccg
     4801 ccgatgaaag gacccacgat tggcgccagc gcggtggacg ccgcccagac gccaaatgcc
     4861 cggccctgct cggcgccctc gaagttggtc gtcaggacgg cgagcgagcc gggcaccagg
     4921 accgcgcccg aagctccctg gaagaggcgc gcgacgatca ggaactcgac gttcggcgcg
     4981 atggcgcaca gcagcgacgt gaccgcgaag ccgatcagcc cgatcacgaa cacgcgcttg
     5041 cggccgtagt agtccgacaa ggcgccggcc agcacgagga acgccgacag cgtcagcagg
     5101 tagccgtact ggatgtagtt ctggccctcg aggacgtcta cgaagagccg cggctcgcgg
     5161 ccgatcgccg gcagcgcgac gctgaggacg gagtaatcga ggaagacgac gctcgaaccc
     5221 aggacagcgg caacgagcgt ccaggtcttg gcagtccagc gggtgggcat cggcggtcaa
     5281 tcctacaatc ggttgcgtgt cccgctcccc gcgcatcgag cagccgtttg cgcgcgacct
     5341 gaaggtcgcg gtcgacgcgg cccgcaaggc gggccagctg cagatggagc gctacgagaa
     5401 gctcgagcgc atcgtccaca agtccgagca cgacgtggtc accgaggtcg acgagctgtc
     5461 cgaacagctg atcatcggtg ccatccgcga tgcctttccc accgattcat tcctggccga
     5521 ggaatcgggc cacagtgccg ggcctgccca gggcggtgaa tcggctgccg acaaatcagt
     5581 cggccgagtg tgggtcatcg atccgctcga cgggaccatc aactacgcca acggcatccc
     5641 gctcttctgc gtggcgatcg gtctgtgtgt cgatggccgg cccacgctgg gtgtggtcta
     5701 cgacccggtg cgcgacgaga tgttcagcgc gatcaccggt ggcggggcca cgctcgacgg
     5761 cacgcccatc cggctgccgg tgaaggagcg cctggtcgac tgcgtcgtct cgctctccct
     5821 gccgcgacgc ggttgggcga agcgcgacca cgcgttgcgg cgcagcatcc gcgtgtcgcg
     5881 ctccatgggc agtgccgcgc tcgccatcgc gtacctgggc aacggtcgct tcgatgcgat
     5941 ggtccagtcc ggtggcatga gtgcctggga cgtgtgcgcc cccggggtga tcgcggcgga
     6001 gggtggggcg cggctgacga cgctcgacgg cggtccgtgg ttcgatatca accgcccgac
     6061 caaatcgatc ggactgctcg gcgcggcgcc ggtccatcac caaacgctct tggagctact
     6121 cgcatgacat ctattccggt tgccggtctc aaccatgccg tgctgtacgt gcgcgacctc
     6181 gacgtcgcgg tcgatttcta tacgagggtc ttcggcttcg aggtggtcga tcggatgcgt
     6241 aacttcgcgt gcttcctgcg cgcggcaggc ggcgcgaacc atcatgacct gggcctcttc
     6301 gccgtaggtc ccgcggcgcc gcgaccgccg cgcggctcga ccggcctgta ccacctcgcc
     6361 tgggaggtgc cgcgcatcga agacctggcg ctggccgcgt cggttctcca ggaagcgggc
     6421 gcgctgggtg gtgcgagcga ccacggcgtg tccaagtcgc tgtatggcca ggaccctgac
     6481 ggcaacgagt tcgagatcat gtggcgcgtc ccgcgcgagc aatggggcga cttggagaag
     6541 gaagcgggca tcgcgccgct cgacttggaa gccgagctcc gaatccatgg cgcgaaagcc
     6601 gccgggtgac gcattggccg gccgggtgga agccatacgc ggaggtccac aagggcgata
     6661 acacgatcac gggcaccgtc ctggtgtggc caaaggttgc gggtgatccg ggcatccctg
     6721 ggcgcgagat ctgtgtctat ctgccgccat cgctcgccag atcgctggcc gcgggggagg
     6781 gcggtgggca tggcggtcgg cgctatcccg ttctctactt catggacggc cagaacgtct
     6841 tcgacgcgaa gacctcgtac atcggcgtcg agtgggcggc cgacgaggcg ctcgagaagc
     6901 tggccgaaga cggcatcgaa gctatcgccg tggccgtggc caaccacggc gacgaccgaa
     6961 tgaacgagta caacccgtgg cgctcgtcgc tcaagtggcg gggccgcgcc caccccacgg
     7021 gtggcaaggg cgacgcgtac ctcgagtggc tggtcggtac cgtcaagcca ttgatcgatc
     7081 gctcgttacc gacacgtgcc gagcgcatgg cgacgggcat cgtcggctcg tcgatgggcg
     7141 gattgatctc gctctacgcg ctattcgcgc agcgtgctgt tttcgggctg gcgggggtta
     7201 tgagcccggc agttgccttc agcgactacc gcgtcgtggc gctcatcgag gaggcgccgc
     7261 tgccgccgac gcgcatccac ctcgacatgg gctggcgcga atggcgcggc gccacgcgcg
     7321 acgcccggaa ggtgcgcgac gccctgctgt cacgcggctt tgagctggat cgcgacctgc
     7381 actacgtcga ggagcgcaac gcgcgtcaca acgaagccgc gtgggcacgc cgcctgcccg
     7441 acgcgctgcg cttcctgctg gccgactttc gcgagccgcg ctagtcgacc cacttcggga
     7501 cgcggccgat caggtcgggc gcggcaagct cgtgcgcgac gcgctcgaac gccgcgcggt
     7561 gcgccccctt ccagcgcatg tcgtcgaggg actggttgac gatcggcacg tcggtgacga
     7621 gcgtcgccag ctcgcggaag aggaacgcca ggtcgaggtg gtcacgcaac gacgccgcga
     7681 gggtggctgc gccgcggacg ttgacatccc agtgcgcgac cttctcgggt atgtcctcga
     7741 ggtggccgta acgcgtcaac agtgcgcctg ctgacacctt gccccagccc gggatgcccg
     7801 ggtaaccatc ggccgaatcg cccaccaggg cgaggaagtc gggaatcgac tcgggtgggc
     7861 agccgaattt ctcgcgcaca ccggcggcat cgatgagctt gcgctggcga cggtcgaatt
     7921 gaacgacctt gccgtcctcg cgcacgcact gacccatgtc cttgtcgggg gtcatgatca
     7981 cgacccgctc gaccccgggc gcgtcggcaa acctcgccgc tgccgtgccc aggccgtcat
     8041 cggcctccca ctcgaccagc ggccagacga cgacacccag ggtggtcagc gcctcctccg
     8101 cgggcgcgaa ctggcgcagc agatcgggtg ccacgccgaa cgaggtcttg tagcccggcc
     8161 agcgctcgtt gcgccacgac tcgatcacgt gatcggacgc gcaacccaaa tgagtcacgc
     8221 cctcctcgcg cagcaggtag aggacgttct cgagtacgcc gatcgtggcg ctcgccgggt
     8281 tacccgccgc gtcgaatctt tgagggcgcg gcgcgaagtg ggcgcggaac agctcgtagg
     8341 tcgcgtcgag caggtggacc tggatgccga tcacgccttc cgtgcgcggc cgacgcccgg
     8401 ggctgcagcg cgatcagccc gagtcgcgac tggcaggagc accaccggtc gcgccgactc
     8461 ggcccgcgcc cgttcactgg cgtagatcgc ggcgccgacg atccccgacg tgttgaggaa
     8521 gcgcgccggc ttgattgggg cgcggctctt gagccatttg tcgtacttct tcatctcctt
     8581 gctgacgccg ccgcctagga tgatcaggtc gggccagaac aggatttcca gccgcgccag
     8641 gaacgcgctg aactcgttcg cccacgcgcg ccagcgcagg tggcgcttct cacgggcgac
     8701 gcctgacagg cgcaactcga cgtcgcgccc cttccattcg agatggccca tctccgtgtt
     8761 gggcacaagc tgcccatcga cgaacagccc actgccgatg cccgtgccca gggtcagcat
     8821 cagcactgtg cccgcgacct cgcggcccgc gccaaagcgg atttccgcca ggcccgccgc
     8881 atcggcgtcg ttgatcgcgt ggacgcgccg cttcagcgcc ttgccgagaa gctcctcggc
     8941 cggggttccg atccagcgct tgtcgatgtt ggccgcggtc atcaccttgc cggccttgat
     9001 cggtccggga atgccgactc cgaccggcag gtcgggtgcc aggtcgcgct ccgccgcgat
     9061 taggcccacc acgtgtagga ctgcctcaat caccgcgtcc gggctcgacg gcttgggcgt
     9121 gttgactcga tggcgccggc tcaacagctc gccggtcgta acgtcaacca gcgccgcctt
     9181 gacaccggta ccgccaatat cgatgcccag cgccgcgcca ggcacggccg gggcaggacg
     9241 cggctgcgcg tcggctcgcc tgtccccggc tttcgtggcc gccttcttgg gcggagtcgg
     9301 gcgttcctgc ttcgctttcg ccttggccat gactgaagga tacggcgcgt aattgacggg
     9361 taagcccgtg gtgagagccc cgggcgagac tcccgcggtg tggacgtctt ccgaatcggc
     9421 tctgctgtgc gcgccatccg gatcaagcgg cattggcggc aggcagacct ggccgccaag
     9481 gcgaacgttc cgagatcagc cgtctccaaa atcgagcgcg gccgtgggcg cgagctgcgg
     9541 cttgacgtcg tgctccgaat cgtcgaggcg ctgggcggcc gattggtcct ccaggtacag
     9601 tggcagggcg gtgatctcga ccggctcctg aacgcccgtc actccgcgtt ccatgagtcg
     9661 gtcgcaccgg ctttcgtcga gctgcccgga tgggtgcttg cgcccgaggt ctcgttctcg
     9721 atccgcggcg agcggggcgt catcgacatc ctcgcctggc acgccgcgtc gcggacgctg
     9781 ttggtgatcg agctcaagac cgagatcgtg gacgtcaacg agctcatggg cacggtcggc
     9841 cggaagcgcc ggcttgccat ggaaatcggc cgtgagcgtg gctggaatgc cgtgcgcgtt
     9901 gccctctggg tggttgttgc tgaaagcaag accaaccgcc ggcgcgtggc caatcacgcg
     9961 atgaccctgc gcgcggcgtt gccggcggac gggcgttcga ttgcgggctg gcttcgcgaa
    10021 cccgaccggc cggtggcctg cctgtccttc tggtcagatg ctcatggtgc gcacactaag
    10081 tccgaccttg ctaccgtcaa gcgcgtgcgg ctgccacgac agactgccgc gtgagcatgg
    10141 ctgctcaggt gtcgaccgct gctggtggga ctatcgcgcg cagcgggagc actaggggca
    10201 gtcggcgcgc ctgcacctgg ccaagtgatc gccggagagg caatagccct cgccggccct
    10261 cccgtggctg gatagtgacc ggttctgggt cgtaaggccc aaatgacccg ccgcccacga
    10321 gataacgatc cggggcgaag caaatggcca tctcggtgca cggcgccgtt gcgccgcgca
    10381 gttccgcagg gttggtatgt cggcactttg gcagacgcgc ccgtaaactg gcccgagcat
    10441 gttcgacgag ttcggcgact tcaagcgcca gctgcccgac cgcgacgcgg acgaaaccaa
    10501 cgactgggtt cattcactcg actcggtcat cgccgacgag ggcaaggcgc gtgcgcagtt
    10561 catc
//
LOCUS       DATBRX010000031         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2577811, whole genome shotgun sequence.
ACCESSION   DATBRX010000031 DATBRX010000000
VERSION     DATBRX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577811"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VIF08_01110"
     CDS             <1..255
                     /locus_tag="VIF08_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134608.1"
                     /translation="VIGIANLVYIIPTQTIFMEITPIELMGRVVAFRSTLVFGSMTLA
                     MGIAGVLAESVPVGLVIAGFGGLTLLGGLIGALLPAVRDP"
     gene            288..692
                     /locus_tag="VIF08_01115"
     CDS             288..692
                     /locus_tag="VIF08_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134609.1"
                     /translation="MTQNDPTLVIAALAVFSAVMVWLIRAITARLSEAGAHVRSLDAR
                     IVNATPQLAGRLSGMRADLAAVSTRTERALWSLSRFDERAEAAREVLAGTRTALDKDR
                     ARLIAARGAIIRIKKSARMIMKALELRRAILG"
     gene            689..1225
                     /locus_tag="VIF08_01120"
     CDS             689..1225
                     /locus_tag="VIF08_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134610.1"
                     /translation="MSDERPVEDELEPADDENAELDADEGLDEDAGVETDAYGDEPQT
                     SSETRATEYVPARPMAQVRGREPRAASPTPARAATAEDELPYIDDRVSKIWVVAMVAV
                     FALILAYGLLFGKAGALTPPTPSPTPSPSPTPTLSATPVPSASQTPAPSITITPLPSS
                     TSVAPTAPATPTPAPSPT"
     gene            1237..1929
                     /locus_tag="VIF08_01125"
     CDS             1237..1929
                     /locus_tag="VIF08_01125"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY8134611.1"
                     /translation="MRRWFRRSKSAGPRVDQDWVARQIEARGIHDPAVLAAMRAVPRE
                     KFVPPDIARQAYDDGALPIGLGQTISQPYIVARMTQALALGDWSAAHAGARPKVLDVG
                     TGSGYQAAILAMLGAEVVSIELEAELAERARLRLMGLGYDVKVVAGDGSRGMPDEAPF
                     AAIVVAAAAPDVPVPLVEQLEPDGRLVLPIGSRLEQVITLIRPTPDGFVREPLEPAVF
                     VPLLGEHGFSGR"
     gene            1945..>2735
                     /locus_tag="VIF08_01130"
     CDS             1945..>2735
                     /locus_tag="VIF08_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY8134612.1"
                     /translation="MTHVFVSPHPDDIALSCGGLVASLRELGQSVTILSVYSGAGGNG
                     GLTDYQRAALGFGTKVNHPNTEAFRRTNIGADYPVSASAVANPPWLADEDRLEVTQQR
                     ANTSARQFWQRAAWTRSANITNAESDTRPLADSIGGQGSLERIDFDAADAAAVRRLED
                     ERYAYFSEASVIFLDLPDAVYRGYEGDGELLGAPRDDDPAPVELLRSEIVRLEPQMVY
                     FPLGIGGHVDHRLCRDVGVALLGEERRWVMPGPGFVGRVSFYEDFP"
BASE COUNT          414 a          940 c          968 g          413 t
ORIGIN      
        1 gtcatcggca tcgccaatct cgtctacatc atccccaccc agacgatctt catggagatc
       61 acgcccatcg agctgatggg tcgcgtggtg gcgttccgca gcaccctcgt gttcggctcg
      121 atgacgctcg cgatgggcat cgccggcgtc ctggcggaga gcgtgccggt ggggctggtg
      181 atcgcaggct tcggggggct cacgctgctg ggcggattga tcggtgccct actgcccgca
      241 gtgcgcgacc cgtagccgcc gcgcggcgag ataattcatc acagaacatg acccagaacg
      301 accccaccct cgtcattgcc gcgctcgcgg tgttcagcgc ggtcatggtc tggctgatcc
      361 gggccatcac cgcccgactg agcgaggctg gcgcgcacgt gcgctcgctt gatgcgcgca
      421 tcgtcaacgc gaccccgcag ctggccggcc gactgagcgg catgcgggcc gatctggccg
      481 ccgtcagcac gcggaccgag cgggcgctat ggtcgttgtc acgcttcgat gagcgcgcgg
      541 aggcggctcg ggaagtcctg gcgggaacac gtacggcgct cgacaaagac agggcgcggc
      601 tcatcgcggc ccgcggcgcg atcattcgca tcaagaagtc ggcgcggatg atcatgaagg
      661 cactggaact gaggagggcg atcctgggat gagcgacgag cgacctgtcg aggacgagct
      721 cgagccagcc gatgacgaga acgccgagct cgacgcggac gaggggctcg atgaagacgc
      781 gggcgtggag acggacgcgt acggcgacga gccgcaaacg agctcagaga ctcgcgccac
      841 ggagtatgtc cctgcccggc caatggccca ggtccgtggc cgagagccgc gtgccgcgag
      901 tcccacgccg gcacgggcgg ccacggccga ggacgagctg ccctatatcg acgaccgcgt
      961 ctccaagatc tgggtcgtgg ccatggtcgc cgtgttcgcg ctgatcctgg cctacggcct
     1021 gctcttcggc aaagcgggcg cgctcacgcc gccgaccccg tcgcccacac cgtcgccgtc
     1081 gccaaccccc accctgagcg caacgccggt gccgtcggcc agccagaccc ccgcgccgtc
     1141 gatcacgatc acgccgctgc cgagctcgac cagcgtcgcg cccacggccc cggccactcc
     1201 gaccccagcg ccgagcccga cctaggccca ggtcccgtgc ggcgctggtt ccgacgctca
     1261 aagagcgctg ggccgcgcgt cgatcaggat tgggtcgcgc gccagataga ggcgcgcggc
     1321 atccacgacc cggctgtgct tgccgccatg cgcgccgttc cgcgcgagaa gttcgtcccg
     1381 ccggacatcg cgcgccaggc ttacgacgac ggggcgctgc cgataggcct cggccagacg
     1441 atctcgcagc cgtacatcgt ggcgcgcatg acgcaggcgc tggcgctggg agattggtcg
     1501 gccgcgcacg ccggggcgcg gccaaaggtg ctcgacgtcg gcacggggtc gggctaccag
     1561 gccgcgatcc tggcgatgtt gggcgcagag gtcgtcagca tcgagctgga ggctgagctc
     1621 gcggagaggg cacggctccg actgatgggc ctgggctacg acgtcaaggt cgtcgccggc
     1681 gacggcagca ggggtatgcc ggacgaagca ccgttcgccg ccatcgtggt cgccgcggcg
     1741 gcgccggacg tgcccgtccc gctggtggag cagctggagc ctgatggtcg gctggtgctc
     1801 ccaatcggca gtcggctcga gcaggtgata acgctcatcc gccccacgcc cgacggcttc
     1861 gtccgcgagc cgctggagcc ggcggttttc gtgcccctgc tgggcgagca tggcttcagc
     1921 ggccgctgat attctcggaa acctgtgacc cacgtcttcg tttcgcccca ccccgacgac
     1981 atcgcgctgt cctgcggcgg gctggtcgcc agcctacgcg agctgggcca gagcgtgacg
     2041 attctctcgg tgtactcggg tgcgggtggg aacggtgggc tgaccgacta ccagcgcgca
     2101 gcactgggct tcgggacgaa ggtgaaccac ccgaacaccg aggccttccg gcggacgaac
     2161 atcggtgccg attacccggt ctcggcttcg gctgtcgcca atccgccctg gctggccgac
     2221 gaggaccggc tggaagtgac acagcagcgc gccaacacgt cggcgcgcca gttctggcag
     2281 cgcgcggcct ggacgcgcag cgccaacatc accaatgccg agtcagacac gcgcccgctg
     2341 gccgattcga ttggcggcca gggctcgctg gagcggatcg actttgatgc cgcagacgcg
     2401 gccgcggtgc gccggctcga ggacgagcgc tacgcgtatt tcagtgaggc gtcggtcatc
     2461 ttcctcgacc tgcccgacgc cgtgtaccgc ggctacgagg gcgacggcga gctgctcggc
     2521 gcgccgcgcg acgacgatcc ggcgcccgtc gagctgctgc ggtctgagat cgtgcggctc
     2581 gagccgcaga tggtctattt cccgctgggg atcggtggcc acgtcgacca ccggctgtgt
     2641 cgtgatgtgg gcgtcgcgct gctgggcgag gagcggcgct gggtgatgcc cggaccgggc
     2701 ttcgtcggcc gcgtgtcgtt ctacgaggac ttccc
//
LOCUS       DATBRX010000032         7609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3248631, whole genome shotgun sequence.
ACCESSION   DATBRX010000032 DATBRX010000000
VERSION     DATBRX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248631"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VIF08_01135"
     CDS             <1..974
                     /locus_tag="VIF08_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134613.1"
                     /translation="REDPGEHDALEGVAAVDKVIEIDQSPIGRTPRSNPATYVGLFTP
                     IRELFAGVPEARMRGYGAGRFSFNVKGGRCENCKGDGVLKVEMQFLPDVYVTCDVCGG
                     KRYNREALEIHYKGRTIADVLDMTLEEALDFFSAVPQIRTKLSTLVEVGLGYMRLGQP
                     ATTLSGGEAQRVKLATELSRRATGKTVYLLDEPTTGLHFADVAKLLHVLHRLVDAGNT
                     VIVIEHNLDVIKTADWIIDLGPEGGERGGEVVVAGTPEKVAATANSATGQYLTRVLKG
                     EPIIPMSNVSFAEAAGRTSPGNGRSPAKNGKARVAVKVEKTATAARR"
     gene            1070..1588
                     /locus_tag="VIF08_01140"
     CDS             1070..1588
                     /locus_tag="VIF08_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134614.1"
                     /translation="MNHQRFFMVIGLSVAVLASCGGGPAATGTPTATGTPGATSTSAP
                     SSTTPAGGATTVNVTVQEFSVLPDVASAPAGDITFSVTNTGPDDVHEFVIIQTDLAPD
                     ALPTDANGAVEEEGEGMTVIDEIEDIAVGANEELTVTLAAGSYALICNIFDESENEAH
                     YQMGMYTAFTVE"
     gene            complement(1575..1973)
                     /locus_tag="VIF08_01145"
     CDS             complement(1575..1973)
                     /locus_tag="VIF08_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEY8134615.1"
                     /translation="MSVLTVLYDADCGVCSHTARLLVRTDKQARLRLVSIQSARLPDM
                     PPRAELMEVLHARDESGRWFQGAAASVEIARRIPIARPLTLYAKLPFAMAILDMMYRS
                     FAANRHAISRMLGLKACQVPAGPRGAATRP"
     gene            2037..2507
                     /locus_tag="VIF08_01150"
     CDS             2037..2507
                     /locus_tag="VIF08_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134616.1"
                     /translation="MPDIEYAFLADAAEARPGQKFAVIGGGVSRLGGPQFPLRHPHLA
                     LVCGLSVTAPEFGREHDLQFMLLTPDGQQLSTANAKVMANGPPDGRDSILTFSLDLWN
                     LTFPAPGDYSIRILVDGSERKRLPLVVEQREAQPGQGRPTGTTEPPFPPPTGQA"
     gene            2511..3182
                     /locus_tag="VIF08_01155"
     CDS             2511..3182
                     /locus_tag="VIF08_01155"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY8134617.1"
                     /translation="MLEFIDKIVIPFLASLYGWLGYGGVVVAMAIESAMIPLPSELIL
                     PFAGFLVSDPTAIEPLTRGGWDFWVVVIAATIGNTIGSVIGYFIGAWGGRPFLERWGK
                     YLLIRPHEIELADHFFQKYGSATAFFSRLLPIVRTFISFPAGVARMNLFKFIVYSTAG
                     AFIWSAALVWAGMQLGARWTDIRHALQPFDLLIAVIVVLLVVLFIWWRLGMPGRRRGE
                     RSANT"
     gene            3169..3729
                     /locus_tag="VIF08_01160"
     CDS             3169..3729
                     /locus_tag="VIF08_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8134618.1"
                     /translation="MPTRDDAWALLCEWTQSDALRKHGRAVEGAVGWYAQNHFGVSGA
                     DLETWRSAGLLHDFDYERFPDQHPLAGVEELRKRGYPEELVAAVMGHGDHTGVPRTTD
                     LAHCVYACDEMSGFVIAVTLVRPNRSLDEVDVHSVQKKMKDKGFARPVPRDQLTKGAD
                     ELGLPFEQHVANVIEGLKTVRADLGL"
     gene            complement(3726..5333)
                     /locus_tag="VIF08_01165"
     CDS             complement(3726..5333)
                     /locus_tag="VIF08_01165"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY8134619.1"
                     /translation="MTLHPDVEARLARLAPDQRAAATAPPGPVLCVAPAGSGKTTTLV
                     ARVCWLVASGADPASITAVTFNKRAAEELGQRLALALEPLSPAASPRVRTFHALGREI
                     LAEARVPVANLVDRADALRALNGGRSVPATDMRRLDDGFSRLKLDAGLDLATAVRSLG
                     EANSKAPDEVDLLKRFVAYEGWLAQRDALDFDDLVRRALRVLRDDAAALAAWRARCAT
                     LLVDEVQDVDRSQLDLAVLLAGERRDIFLVGDDDQTIYAWRLADVRRVLDLAGALPGL
                     RRVDLVTNYRCPRPVVERAVRLISRNRERFAKSIRARVGAAGTLVLAPDSGDGLDRAR
                     RLLSAWLPAASGVHAVLARTNAELAPYAAVALERGTPYRAAEDGLLLDDPVIDTLTAA
                     LPANERPTIALARQDGQGNRLARSLAGWALPYPSVAELRVAIAAARRRRAELRQNDAP
                     LVLATAHGTKGLEFDHVAVIGMDEHVFPSRRTLEEADDPARALEEERRLAYVAWTRAR
                     QSLTLVYDPTAPSIFMREAFSPAELAA"
     gene            5585..7303
                     /locus_tag="VIF08_01170"
     CDS             5585..7303
                     /locus_tag="VIF08_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134620.1"
                     /translation="MPIMVLLVATMTAAPVAAARPEANFITDQDAMLWNLAGGSVEPI
                     LTVGDEMGSYMFDAIPDGISVASWGSNSVKVFVNHETSTVPFPYSTSSPGGNLNDFTD
                     SLVSKLRINRSTHKVTAASLILDDGDDVHRLCSNFLATSIHGFDRPILLTNEEGIDWV
                     NRAGLQWPATEGADTARQIGAVVARDLGTGVNRIIWGMGRHNHENSLPLKGYGYPVVL
                     SGDDSFVTSPNQSQVYSYIADSRADLWNDEGELWAFVSDDLAYDDYYDFVPGSTHSVT
                     GHFIQVPKEIATGRNPDGSDMMAADVPAEFGGPYDPPPSDGAWQRPPGATSGPGVDGP
                     QWVLETWSDEHNVFSFVRVEDMAYDKRQGMRNVVYIVDSGRGRGPSDSAGLDSDGNAV
                     VNKSTNGRIWKMVFDKNDPTVVTSLSILIEGDDSPTKTLTEIRQPDNIETTKKGIYIT
                     EDPGSSQQFAPGDLAGKNARVWQYKFGAVPTLNPVLEVNQDADEGDTDVDSSSEGSMG
                     AWEASGIIDVSQWFGPGMFLITVQAHTLFIETAPGPDINDPPTGIDWTYKREGGQLLL
                     VKIPGG"
     gene            7413..>7609
                     /locus_tag="VIF08_01175"
     CDS             7413..>7609
                     /locus_tag="VIF08_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY8134621.1"
                     /translation="MTPQLQATLAKLPTKPGVYLLKDKNGRVLYVGKAQSLRNRVRQY
                     WQAGRGAQAPLRIESAIDRVA"
BASE COUNT         1279 a         2598 c         2531 g         1201 t
ORIGIN      
        1 cgcgcgagga cccgggcgag cacgacgcgc tcgagggcgt cgccgcggtc gacaaggtca
       61 tcgagatcga tcagtcgccc attggccgca cgccgcgctc caacccggcg acttacgtcg
      121 ggctatttac gcccatccgc gagctgttcg cgggtgtgcc ggaggcgcgc atgcgcggct
      181 acggcgccgg ccgcttctcg ttcaacgtca agggcggccg gtgcgagaac tgcaagggcg
      241 acggcgtgct caaggtggag atgcagttcc tgcccgacgt gtacgtcacg tgcgacgtgt
      301 gcggcggcaa gcgctacaac cgcgaggcgc tggagatcca ctacaagggc cgcacgatcg
      361 cggacgttct cgacatgacg ctcgaggagg cgctcgactt cttcagcgcc gtgccccaga
      421 tccgcaccaa gctctcgacg ctggtcgaag tgggattggg ctacatgcgc ctgggccaac
      481 ccgcgaccac tctgtcgggc ggcgaggcgc agcgcgtcaa gctcgcgacc gagctatcac
      541 gccgcgcgac gggcaagacc gtgtacctgc tcgacgagcc gaccacgggc ttgcacttcg
      601 ccgatgtcgc gaagctgctc cacgtgctgc atcgactcgt cgatgccggt aacaccgtca
      661 tcgtgatcga gcacaacctc gacgtgatca agaccgccga ctggatcatc gatctcgggc
      721 cggagggcgg cgagcgcggc ggcgaggtcg tcgttgccgg aacgccggag aaagtcgcgg
      781 ccactgccaa ttccgcgacc ggtcagtacc tgacgcgcgt cctcaagggc gagcccatta
      841 tccccatgag caacgtctcc ttcgcggagg ccgccggccg gacgtcgcct ggcaacggca
      901 ggtcgcccgc caagaacggc aaggcccgag tcgccgtcaa ggtcgagaag acagcaaccg
      961 ccgcgcgccg ctaactgcag cgtcttttcg atgttctgag ccccggcgct tattcggctg
     1021 gctgccgtgc ctcgcaccct tgtgctttac aagagcggga ggacccacga tgaatcacca
     1081 acgcttcttc atggttatcg ggctgtcggt ggccgtgctt gccagttgcg ggggcggccc
     1141 agccgcgacg ggcacgccga ccgcgacggg cacgccgggg gcgacttcga cgagcgctcc
     1201 gtcatcaaca acgccggcgg ggggggccac caccgtcaac gtgacggtcc aggagttctc
     1261 cgtgctgccg gacgtggcct cggcacccgc gggcgacatc accttcagcg tcaccaacac
     1321 aggacctgat gacgtgcacg agttcgtcat cattcagacc gacctcgcac ccgatgctct
     1381 gccgaccgac gcaaacggcg cggtcgagga ggagggcgag ggcatgacag tgatcgatga
     1441 gatcgaagac atcgcggtcg gggcgaacga ggagctcacc gtcaccctgg cggccggtag
     1501 ctacgcgctg atctgcaaca tcttcgatga atcagagaac gaggcgcact accagatggg
     1561 catgtacacc gcgttcacgg tcgagtagcc gcccctcgtg ggccggcggg aacctggcac
     1621 gccttgaggc cgagcattcg gctgatggcg tgccggttgg ccgcgaacga gcgatacatc
     1681 atgtccagga ttgccatcgc gaacggcagc ttcgcgtaaa gggtgagcgg tcgcgcaatc
     1741 gggatgcgcc gcgcaatctc gacgctcgcg gccgcgccct ggaaccagcg tccgctctcg
     1801 tcgcgggcgt ggagcacttc catcagctcg gcccgaggcg gcatatcggg tagccgggcg
     1861 gactgaatcg agacgagacg gagtcgggcc tgtttgtcgg tgcgtaccag cagccgcgca
     1921 gtgtgcgagc acactccgca atcggcgtcg tacaggaccg tcagcacgct catggggcgc
     1981 gccggactgt cgccatgttc tctgcctcgc ccatcgcggc ccctagacta cgccgcgtgc
     2041 cggacatcga atatgcattc ctggctgacg ccgcggaggc gcggccgggc cagaagttcg
     2101 cggtaattgg tgggggagtg tcccgccttg gcgggccgca gtttccgttg cgccatccgc
     2161 atctggcgct ggtgtgcggc ctgtccgtga ctgcgcccga gttcggccgc gaacacgacc
     2221 tgcagttcat gctgcttacg cccgacggcc agcagctctc cactgccaac gccaaggtca
     2281 tggccaacgg cccgccggac ggccgtgaca gcatcctgac cttcagcctc gacctgtgga
     2341 acctcacgtt cccagcgccg ggcgactact cgattcgcat cttggtcgat ggctcggagc
     2401 gcaagcgctt gccgctcgtg gtcgagcagc gcgaggcgca gcccgggcag ggccggccga
     2461 cgggcaccac ggagccgccc ttccccccgc cgaccgggca ggcataggca atgctcgaat
     2521 tcatcgacaa gatcgtcatc ccgtttctgg cgtcgctcta cggctggctg ggctacggcg
     2581 gcgtggtcgt cgccatggcg atcgaatcgg cgatgatccc gctcccgtcc gagctgatcc
     2641 tgccgttcgc cgggttcctg gtctccgatc cgacggccat cgaaccgctt acgcgtggtg
     2701 gctgggattt ctgggtggtc gtgatcgcgg caacaatcgg caacaccatc ggatcggtca
     2761 tcggctactt catcggcgcg tggggcggcc ggccgttcct ggagcgctgg ggcaagtacc
     2821 tgctcattcg gccgcacgag attgagctgg ccgatcattt cttccagaag tacggctcgg
     2881 ccactgcgtt cttcagccgc ctgctgccga tcgtccgcac attcatctca ttcccggccg
     2941 gggtggcgcg catgaacctg ttcaagttca tcgtgtactc gacggccggg gcgttcatct
     3001 ggtcggccgc gctcgtctgg gccggcatgc agcttggtgc gcgctggacc gacattcgcc
     3061 acgcccttca gccgtttgat ctgctgatcg cggtcatcgt cgtgctgctc gtcgtgttgt
     3121 tcatctggtg gcgactgggc atgcccggtc gccgccgcgg ggagcgcagt gccaacacgt
     3181 gacgacgcct gggcgttgct gtgcgagtgg acgcagtctg acgcgctgcg caagcacgga
     3241 cgggccgtag agggcgcagt cggctggtac gcgcagaacc acttcggagt gagtggtgcg
     3301 gacctggaaa cgtggcggtc ggcgggcctg ctgcacgact tcgactacga gcgcttcccc
     3361 gatcaacacc cgctcgccgg cgtcgaggag ctgcgcaagc gcggctaccc cgaggagctc
     3421 gtcgccgcgg tcatgggtca cggtgatcac acgggtgtgc cgcgcacgac tgacctggcc
     3481 cactgcgttt atgcctgcga cgagatgagc ggctttgtca tcgcggtcac gctcgtccgg
     3541 ccgaatcgca gcctcgacga ggtcgacgtc cactccgtcc agaagaagat gaaggacaag
     3601 ggcttcgccc gcccggtgcc gcgcgaccag ctgacgaagg gcgccgatga gctgggcctt
     3661 cccttcgaac agcacgtcgc caacgtcatc gagggcctga agacagttcg tgccgatctg
     3721 ggcctctagg ccgcgagctc ggcggggctg aaggcctcgc gcatgaagat cgacggcgcc
     3781 gtagggtcgt agacgagcgt cagcgactgg cgagcgcgcg tccacgctac gtaggcgagg
     3841 cgacgctcct cctcgagcgc gcgcgccggg tcatccgcct cctcgagcgt ccgccggctg
     3901 gggaatacgt gctcgtccat gccgatcacc gcgacgtggt cgaactccag gcccttggtg
     3961 ccgtgcgccg tggccagcac gagcggcgcg tcgttctggc gcagctcggc gcggcgccga
     4021 cgcgcggcgg cgatcgcgac gcgtagctcc gcgaccgacg ggtaggggag tgcccagccg
     4081 gcaaggctgc gcgccagcct gttgccttgc ccgtcctggc gggccagcgc gatagtcggc
     4141 cgctcgttcg ccggtagcgc cgcggtcagc gtgtcgatca ccgggtcgtc aagcagcagc
     4201 ccgtcctctg cggcgcggta cggggtgccg cgctccaacg cgacggccgc atacggggcg
     4261 agctcggcgt ttgtgcgcgc cagcacggcg tgtactcccg acgcggccgg caaccaggcg
     4321 cttaacagcc gccgggcacg atcgaggcca tcgccgctgt ccggtgccag cacgagcgtc
     4381 ccggccgcgc cgacccgggc gcggatcgac ttggcgaagc gctcccggtt gcggctgatc
     4441 agccgcacag cgcgttcgac gaccggccgc ggacagcggt agttcgtcac gaggtcgacc
     4501 cgccgcaacc cgggcagcgc gccggccagg tcgagcacgc gccgtacatc ggccagccgc
     4561 caggcgtaga tcgtctggtc gtcgtcaccg acgaggaaga tgtcgcgccg ctcgccggcg
     4621 agcagcaccg cgagatcgag ctgcgagcgg tcgacgtcct ggacctcgtc aaccagcaac
     4681 gtcgcgcagc gtgcccgcca cgcggcgagt gcggccgcgt catcgcgcag caccctcagg
     4741 gcgcgacgga ccaggtcgtc gaagtcgagc gcgtcgcgct gcgcgagcca gccttcgtag
     4801 gcgacgaagc gcttcagcag gtccacttcg tcaggcgcct tcgaatttgc ctcccccagt
     4861 gaccgcaccg cggtcgccag gtcaaggccc gcatccagct tcaggcgcga gaatccatcg
     4921 tcgagccggc gcatgtccgt ggccggtacg gatcgcccgc cgttgagggc ccgcagcgcg
     4981 tcggctcggt cgaccaggtt ggcaaccggc acgcgtgcct ctgccaggat ctcgcgcccg
     5041 agagcgtgga acgtccgcac gcgcggcgaa gccgccggcg acagaggctc cagcgccagt
     5101 gcgaggcgct ggcccagctc ctcggccgcg cgcttgttga aggtgaccgc cgtgatcgac
     5161 gccgggtccg cgccggacgc gacaagccag cacacgcgcg ccacgagcgt cgtcgtcttg
     5221 ccgctgcccg ccggggcgac gcacagcacc ggcccgggcg gcgccgtcgc cgctgcgcgc
     5281 tgatcagggg cgaggcgggc gagccgggcc tcgacatcgg ggtgcagggt cacccgctcg
     5341 actatggcga tgccccctta tcgatgggtt atcggcgggt ccatcgccag agacgttgca
     5401 gttagcgagt gcctgttccc atccgccacc ggaccgaata acctccgccg gtcagcgtca
     5461 gccgggcacc gcgctcagcc cgttttgacc acctgacggg aggcgccaga tgcccacatg
     5521 ggagccggcg cggaggagat ggatgcccaa gatccaacgc ggccttgtcc gtagacgcgc
     5581 gctcgtgcca ataatggtcc tcctggtggc caccatgacc gcagcgcccg tcgccgctgc
     5641 tcggccagag gccaacttca tcaccgatca ggacgccatg ttgtggaacc tggcgggtgg
     5701 ttcggtcgaa ccgatcctca ccgttggtga cgagatgggc agctacatgt tcgatgcgat
     5761 tcccgatggg atcagcgtcg ccagctgggg tagcaactcg gtcaaggtgt tcgtcaacca
     5821 cgagacctcg accgtgccgt tcccgtacag cacctcatca ccgggcggca acctgaacga
     5881 cttcaccgac tcgctcgtca gcaagctgcg catcaaccgc agcacccaca aggtcaccgc
     5941 cgcgagtctc atcctcgatg acggcgacga cgttcatcgt ctgtgctcga acttcctggc
     6001 gacaagcatt cacggcttcg accggcccat cctgctgacc aacgaggaag gcatcgactg
     6061 ggtcaatcgt gccgggctgc agtggccggc gaccgaaggt gccgacacgg cccgccagat
     6121 cggcgcggtg gtggcgcgtg acctcggaac cggcgtcaac cggatcatct ggggcatggg
     6181 ccgacacaac cacgagaact cgctgcccct gaagggctac ggctatccgg tcgtgctctc
     6241 gggtgacgac tcattcgtga ccagccccaa ccagtcgcag gtctactcat acatcgccga
     6301 cagccgggcg gatctctgga acgacgaggg cgagctgtgg gccttcgtct ctgacgacct
     6361 ggcatacgac gactactacg acttcgtgcc cggctcgacg cacagcgtca ccggccactt
     6421 catccaggtg cccaaggaga tcgccaccgg gcgcaatccc gacggctccg acatgatggc
     6481 tgcagatgtg cctgccgagt tcggcggacc ctatgacccg ccgccctccg acggggcttg
     6541 gcagcgcccg cccggcgcga ccagcggccc aggcgtggat ggcccgcagt gggtcctcga
     6601 gacgtggagc gacgagcaca acgtcttctc cttcgttcgc gtcgaggaca tggcgtacga
     6661 caagcgccag ggcatgcgca acgtcgtcta catcgtcgac tccggccgcg gccgcggtcc
     6721 atctgacagc gccggcctgg attcggacgg caacgcggtt gtcaacaagt caaccaacgg
     6781 tcgcatctgg aagatggtct tcgacaagaa cgacccgaca gttgtcacgt ccctctcgat
     6841 cctgatcgaa ggcgacgaca gcccgaccaa gacgctgacc gagatccgcc aaccggacaa
     6901 catcgagacc accaagaagg gcatctacat caccgaggat ccgggcagca gccagcaatt
     6961 cgcaccgggc gacctggccg gcaagaatgc ccgcgtctgg cagtacaagt tcggcgctgt
     7021 gccgacgctc aaccctgtcc tcgaggtcaa ccaggacgcc gacgagggcg ataccgacgt
     7081 tgatagctcg agcgagggca gcatgggcgc ctgggaggcg agcggcatca tcgacgtctc
     7141 gcagtggttc ggtccgggga tgttcctgat caccgtccag gcccacaccc tgttcataga
     7201 aaccgcgccc ggtccggata tcaatgaccc gccaacgggc attgactgga cgtacaagcg
     7261 cgagggcggt cagctgttgc tggtgaagat cccaggcggc tgatagcggc aaattcgctg
     7321 aagtaacagg agcggccccg accgaaaggc ggggtcgctt ccttgtttcc gtggggcata
     7381 cccgtgttgc cgcgctacga tcgaatgacc cgatgacccc gcaactccag gcgacgctgg
     7441 ccaagctgcc caccaagccc ggcgtatacc tgctcaagga caagaacggc cgcgtgctct
     7501 acgtgggcaa ggcgcaaagc ctgcgcaacc gcgtgcgcca gtactggcaa gctggtcgcg
     7561 gcgcgcaggc gccgctgcgc atcgagtcgg cgatcgatcg cgtggccga
//
LOCUS       DATBRX010000033         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2914655, whole genome shotgun sequence.
ACCESSION   DATBRX010000033 DATBRX010000000
VERSION     DATBRX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2914655"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VIF08_01180"
     CDS             <1..346
                     /locus_tag="VIF08_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134622.1"
                     /translation="RAGLTRSRDKRVYASAALLLAEGAALVVGRGDCPFGPFQAGLGD
                     PVPMFEWFLPPRAAKAAIPALTLVTVCGLMVLWLRPPEPRQSATWSWRAARVTNTSRA
                     SATLGPMRSPIA"
     gene            265..663
                     /locus_tag="VIF08_01185"
     CDS             265..663
                     /locus_tag="VIF08_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134623.1"
                     /translation="MVMARGPGDEYESGVCNIGPDEIAHRLTLAHIGLLITLGFLALL
                     IVTHVPLLARFSVALPAWGTAVAYLQAWRRFCVAFGTLGVFNFGQVGSSERVADPQMR
                     ARDRRRALEIVLLGLIAGVVAGTVAVLLPI"
     gene            complement(666..1595)
                     /locus_tag="VIF08_01190"
     CDS             complement(666..1595)
                     /locus_tag="VIF08_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY8134624.1"
                     /translation="MPMPHQTFAQMAKRRLVGKEGKERIRVVRELIAQLPDYRNGPYA
                     DLRKSLLAEIEETRVRSNAVHRDSIAVRREGAAQIALVGPPNVGKSSVLQALSEIQIK
                     TGDYPFTTLRPVPALTRVKGVLVQLVEIPGLIGGAADDRGGGRALLGVLRSADAIVFC
                     ARAGYPPDELQTVREELARAEIEKPAVLAVTRADEATDADIGRLAQAFPDLQMVRVSV
                     LDPASLDALRDAIWSLTGLIRIYLRTNGSTQSEPLALDPGATVADVADSVHHELGASF
                     TAARVWGPSARFEGQRVGREHAVQDTDTVEILK"
     gene            complement(1888..>3417)
                     /locus_tag="VIF08_01195"
     CDS             complement(1888..>3417)
                     /locus_tag="VIF08_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8134625.1"
                     /translation="VAGTIAGIGAASGGKWVGVAPGADIYMFRGGAGLNIFSWWAVRA
                     FDWVIQNGKALNIRVISNSWGGGDGSDYDPNDPVNIMSKAAYDHDIVVVFAAGNSGGP
                     NMLGSNSVSPYVICVGAVDKSGAKASFTSTGRPGGDMTRDANGLYRPTVVAPGVDIIA
                     PHSSTGAVMADGLDTSNPFYTHSDGTSMATPHVAGVVALMLQVRPSLTPQNVIDILEG
                     TATSLKSYERWQVGEGLVNAYAAIKAAEKGQVKFHPSTSGKTPQYKLLLSADWNGKVL
                     PAGYSVIDQTNALASDTPVPVAKGADALYAEIEWASPNESVYLQLLDPSGKLVEESAG
                     LTDIGFVNFRSVVTTSPVAGTWTVRVVGRINAVTDYRGFWGTYQLTTNYKAPSTTNLT
                     TVTKPYSGTSNTSADAVHDSQYFTISVPTGATQVSATLDWADTNYDLDLYLFDATGRL
                     VKSSTNGDVNHEELAVTSGTDPMMPAAGLPAGTWTLEVRGWLVVAPEAFTGTYSVTYP
                     K"
BASE COUNT          501 a         1143 c         1179 g          594 t
ORIGIN      
        1 gcgagccggc ctgacccgca gccgcgacaa gagggtctac gcctctgccg cgcttctgct
       61 ggccgaaggc gccgcgctgg tcgtcggccg gggagattgc ccgttcggtc ccttccaggc
      121 gggcctgggc gaccccgtgc ccatgttcga gtggttcctg ccaccgcgcg ccgcgaaggc
      181 ggccatacct gccctgacat tggtcacggt ctgcggcctg atggttttgt ggctgcgccc
      241 gcctgagccg cgacaatcag cgacatggtc atggcgcgcg gcccgggtga cgaatacgag
      301 tcgggcgtct gcaacattgg gcccgatgag atcgcccatc gcctgacgct agcccacatc
      361 gggctgctga tcacgctcgg ctttctcgcg ctgctgatcg tcacccacgt gccgctgctc
      421 gcgcgcttca gcgtcgcgct acccgcatgg ggcacggcgg tcgcctatct ccaggcgtgg
      481 cgcaggttct gcgtggcctt cgggacgctg ggcgtcttca acttcggcca ggtcggctcg
      541 agcgagcgcg tcgcggaccc gcagatgcgc gcccgcgatc gccgtcgcgc cctggagatc
      601 gtgttgctcg ggctgatcgc gggggtggtg gcgggcaccg tggcggtgct gctcccgatc
      661 tgaacctatt tcaggatctc gaccgtgtcc gtgtcctgta ccgcgtgctc ccgccccacg
      721 cgctgacctt cgaagcgcgc tgacggcccc cacacccgag cggcggtgaa ggacgcgccg
      781 agctcgtgat ggaccgagtc ggctacatcg gcgacggtcg ctccgggatc cagcgccagc
      841 ggttccgact gggtcgagcc gttcgtgcgc aggtagatcc tgatcagtcc ggtcaggctc
      901 cagatcgcgt cgcgcagcgc gtcgagtgaa gcgggatcaa gcaccgacac ccgcaccatc
      961 tgcagatcgg ggaacgcctg ggcaagtcgg ccgatgtcag catcggtggc ttcgtcggcc
     1021 cgggtcaccg ccagcacggc gggcttctct atctcggccc gggcgagctc ctcgcgcacc
     1081 gtctggagct cgtccggcgg atagccggcg cgagcgcaga agacgatcgc gtccgctgag
     1141 cgcagcacgc cgagcagcgc ccggccaccg ccgcggtcat cggctgcgcc gccaatgagg
     1201 ccgggaatct caaccagctg cacgagcaca cccttgacgc gggtgagcgc cggcaccggc
     1261 cgcagcgtcg tgaacgggta gtcgccggtc ttgatctgga tctctgacag ggcctgcagg
     1321 acagagctct tgcccacgtt gggcggtccg acaagggcga tttgcgcggc accctcgcgg
     1381 cgcacggcga tcgagtcgcg gtgaacggcg ttcgaccgga cgcgcgtctc ctcgatctcc
     1441 gcgaggagcg acttgcgcag gtccgcgtac ggcccgttcc tgtagtcggg cagctgcgcg
     1501 atcagctcgc gcacaacgcg aatgcgctcc ttgccctctt tcccgaccag ccgacgcttc
     1561 gccatctggg cgaacgtctg atgcggcatc ggcatctgtt catctcctgt gactgactac
     1621 cacgcaggag tgcacgcgcg cgcccgaccg aagggcgctg gcggcgtgcg tcgctcctgc
     1681 cccttcctcc gacgcagttg cggaaggggc cgatcagccg ttctgacggg agatggtggc
     1741 gtaccaggcg agtgacatgt cgccgggtag ggtagcggag tcgtcggcgc aaaacgcgca
     1801 ctccgactct gggtgggcca ccggacatga agaggccggc gccccgtgcg ggacgccggc
     1861 ctccattgcc agatgggatt ggcgcggcta cttggggtac gtcaccgagt acgtaccggt
     1921 gaacgcctcg ggcgcgacca cgagccagcc ccggacctcg agtgtccagg tgccggcggg
     1981 gagacccgcg gcaggcatca tgggatcggt gcccgatgtg accgccagct cctcgtggtt
     2041 cacgtcgccg ttggtcgagc tcttgacgag ccgaccggtg gcgtcgaata ggtagaggtc
     2101 gaggtcatag ttcgtgtctg cccagtcgag cgtcgcgctg acctgcgtgg cgcccgtcgg
     2161 aacagagatg gtgaagtact gcgagtcgtg caccgcgtcc gccgacgtgt tcgaggtgcc
     2221 gctgtacggc ttcgtcaccg tcgtgaggtt ggtcgtgctc ggtgccttgt aattggtcgt
     2281 gagctgatac gtgccccaga agccgcggta gtcggtcacc gcgttaatgc ggccgaccac
     2341 gcgcaccgtc cacgtaccgg cgaccgggct cgttgtcacg accgagcgga agttgacgaa
     2401 gccgatgtcc gtcagcccgg cggactcctc gaccaacttg ccgctgggat cgagcagctg
     2461 caggtagacg ctctcgttgg ggctcgccca ctcgatctcg gcgtacaggg cgtcagcgcc
     2521 cttggccacc gggaccgggg tgtcggatgc cagcgcgttc gtctggtcga tcacgctgta
     2581 cccggcgggt aggaccttgc cgttccagtc ggcgctcagg agaagcttgt actggggcgt
     2641 cttgccgctc gtcgagggat ggaacttgac ctggcccttc tcggcggcct tgatagccgc
     2701 gtaggcattg accaggccct cgccgacctg ccagcgctcg tagctcttca ggctggtggc
     2761 cgtgccctcg aggatgtcga tcacgttctg cggcgtgagg ctcgggcgaa cctgaagcat
     2821 cagcgcaacc acgcccgcaa cgtgcggcgt ggccatgctc gtgccatcgg agtgggtgta
     2881 gaacgggttc gacgtgtcga gcccgtcggc catcacggca ccggtcgatg aatgcggtgc
     2941 gattatgtcg acgccgggcg cgacgaccgt tggccgatac aggccgttgg cgtcccgcgt
     3001 catgtcgccg cccgggcggc cagtcgaggt gaaggacgcc ttggcgccgc tcttgtcaac
     3061 tgcgccgacg cagatcacgt agggcgaaac cgagttgctg ccgagcatgt tcgggccacc
     3121 cgagttgccg gctgcgaaga ccaccacgat gtcgtggtcg taggcggcct tggacatgat
     3181 gttcaccggg tcgttggggt cgtagtcgga gccatcgccg ccgccccagc tgttcgagat
     3241 cacgcggatg ttgagcgcct tgccgttctg gatgacccag tcgaacgcgc gcactgccca
     3301 ccacgagaag atgttcagac cggcgccacc gcggaacatg tagatgtccg cgccgggggc
     3361 gacgccgacc cacttgccac cgctcgcggc gccaatgccg gcgatcgtgc ccgcgac
//
LOCUS       DATBRX010000034         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1016702, whole genome shotgun sequence.
ACCESSION   DATBRX010000034 DATBRX010000000
VERSION     DATBRX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016702"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VIF08_01200"
     CDS             complement(<1..182)
                     /locus_tag="VIF08_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEY8134626.1"
                     /translation="MERYFGRLAKLISRATGHPVAFVIALAVVVGWALSGPIFGFSDT
                     WQLVINTGTTIATFLMV"
     gene            complement(304..693)
                     /locus_tag="VIF08_01205"
     CDS             complement(304..693)
                     /locus_tag="VIF08_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY8134627.1"
                     /translation="MPAYLDSSAAVKLVFDESESEALQVYVGSSPRWASSALIRVELL
                     RAAKRLDARRVGRAYELLGSISIREIDDEILDRAAEISPLNIRTLDAIHLATATLMGP
                     NLEVLVTYDRRMIEAAQLYGLPIASPA"
     gene            complement(693..971)
                     /locus_tag="VIF08_01210"
     CDS             complement(693..971)
                     /locus_tag="VIF08_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY8134628.1"
                     /translation="MTRIGIRELRQNASEYVRRAEKGETIEVTDRGRPVAQLAPLPQP
                     EDILERWIAEGKATRPTGHLANLGPPVPLPPGSPSTQEVLDELREERI"
     gene            989..>2728
                     /locus_tag="VIF08_01215"
     CDS             989..>2728
                     /locus_tag="VIF08_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8134629.1"
                     /translation="MSCPATMRAMTRDWPTFGRDVADAAWRPAANAPDATRLGRFIRA
                     TREPHLESLQRHSERDPAWFWGAAADDLALDWQRRPTQTLDTSGGVEWSRWWSGGAFN
                     YASAAIDGRAQRDPAGAALTWEGEDGTVRRFTNAELKAATDRAAAMLARLGVREGDRV
                     GIFLPLLPETVMTVLALGKLQAIYIPIFSGYAAPAVASRLNDAGATLLVTADGTLRRG
                     GKVNLKETADAAVAQSPTVRTVLVVGRLSPFPPEQGWVENRDRWWQSEMADPTLQPIT
                     ETPTTDPETPYMIIYTSGTTGRPKGAVHVHGGFPIKGAQDLAHCFDLGARDKLFWFTD
                     LGWMMGPWAISGALLLGAELVLYEGAPDHPQPDRLWALVARHGVTHLGLSPTVIRALM
                     AHGTDPIRGHDLSSLRVLGSTGEPWNPESWWWFFREIGGGRLPLINYSGGTEVSGGIV
                     ACNLMTPIKPTSFGGPNVGAAADVIDATGNQVRGEVGELVLRQPLPGMTRGFWQDRER
                     YLETYWSRIPRTWVHGDWAIVDEDGYWFIQGRSDDTLKVAGKRVGPAEVESAAVAHPA
                     VLEAAAVGVPHDIK"
BASE COUNT          463 a          909 c          929 g          427 t
ORIGIN      
        1 accatcagga acgtcgcgat cgtcgtgccg gtgttgatca ccagctgcca ggtgtcggag
       61 aagccgaaga tcggcccgct gagcgcccaa cctacgacaa ccgccaaggc gatgacaaac
      121 gccacgggat gacccgtcgc acgcgagatc aacttggcca gtcgtccgaa atagcgttcc
      181 actgcgcacc tcagctcggg ggattcggaa tgtcgccgcg agagcgatgc acgctcatcg
      241 attcgccctg cgccgtgact tgcgaacgcg ctgcaaagca cgaagggacg gcgtaaccgg
      301 cggtcaggcc ggcgaggcga tcggcagtcc gtacagctgc gcggcctcga tcatgcgccg
      361 gtcgtaggtg accagcacct caaggttggg gcccatcagc gttgcagtgg cgagatgaat
      421 cgcatcgagc gtgcggatat tgagcggcga gatttccgca gcgcgatcga ggatctcgtc
      481 gtcaatttca cgaatgctga tcgaacctaa gagctcatag gcgcgtccga cccgacgggc
      541 atcaagtcgt ttcgcagcgc gcagcagctc cacgcggata agcgcggacg acgcccatcg
      601 tggcgaagac cctacataca cctgcagagc ctctgactcg gattcatcga ataccagctt
      661 gacggcagcc gatgagtcga gataggccgg cactagattc tttcctcacg cagctcgtcg
      721 aggacctctt gggtagatgg cgagcctggc ggcagtggca ccggcgggcc gagattcgcg
      781 aggtggccgg tagggcgtgt tgccttgccc tcagctatcc atcgctcgag aatgtcttcc
      841 ggctggggca gtggagcgag ctgtgcgacg gggcggcccc ggtcggtcac ctcgatggtc
      901 tcgcccttct ctgcgcggcg cacgtactcg ctcgcgttct gccgcagctc ccttatgccg
      961 attcgtgtca tgtgctacat cgtagcacat gagctgtccg gctacgatgc gggcgatgac
     1021 ccgcgactgg ccgacctttg ggcgcgacgt ggctgacgct gcctggcgac cggccgcgaa
     1081 cgccccagac gcgacacgcc tgggacgctt cattcgagcc acgcgtgagc cacacctgga
     1141 gtcgctccag cgccactctg agcgcgaccc cgcgtggttc tggggcgcgg ccgcagacga
     1201 tctggctctc gactggcagc gccgcccgac gcagaccctc gacacgagcg gcggcgtcga
     1261 gtggtcgcgc tggtggagcg gcggcgcgtt caactacgcg tcggccgcga tcgatggccg
     1321 agcccaacgc gaccccgccg gcgcggcact cacctgggag ggcgaggacg gcaccgtccg
     1381 tcgtttcacc aacgctgagc tgaaggccgc aacggatcgc gcggcggcaa tgctcgcgcg
     1441 gctgggcgtc agggaaggcg accgcgtggg catcttcctg ccgctattgc cagaaacggt
     1501 catgaccgtc ctggcgcttg ggaaactgca agcgatctac atcccgatct tctccggcta
     1561 cgcggccccc gctgtcgcga gcaggctcaa cgacgccggt gccaccttgc ttgtcaccgc
     1621 cgacggcacg cttcgtcgcg ggggcaaggt caacttgaag gagacggcgg acgccgcagt
     1681 cgcccagagc ccgaccgtca ggacggtgct ggtagtcggc cgcctgagcc ctttcccgcc
     1741 cgaacagggc tgggtcgaaa acagggatcg ctggtggcag tcggagatgg ccgacccgac
     1801 ccttcagccg atcaccgaga cgccgacgac ggaccccgag acgccgtaca tgatcatcta
     1861 cacgtcgggc acgaccggcc ggcccaaggg cgcggtccat gtccacggcg gcttcccgat
     1921 caagggcgca caggacctgg cgcactgctt cgacctgggc gcgcgcgaca agctcttctg
     1981 gttcacggac ctgggctgga tgatgggccc gtgggccatc agcggagccc tgctgctcgg
     2041 cgccgagctg gtgttgtacg aaggtgcccc cgaccacccg cagccggacc gcctctgggc
     2101 gctggtggcg cgccacggcg tgactcacct gggcctctcc cccaccgtca tccgcgcgct
     2161 gatggcgcac ggcacggacc cgattcgggg ccatgacctg agctccctgc gcgtactggg
     2221 gtcgacaggc gagccgtgga acccggagtc ctggtggtgg ttcttccgcg aaatcggtgg
     2281 cggtcgtctg ccgctcatca actacagcgg cgggacggag gtgagtgggg ggatcgtcgc
     2341 ctgcaacctg atgacgccga tcaagccgac gtcgttcggc ggccccaacg tgggcgcggc
     2401 cgcggacgtc atcgacgcga cgggtaacca ggtccgcggg gaagtcggcg agctcgtgct
     2461 gcgccagccg ctgcccggaa tgacaagggg cttctggcag gaccgcgagc gctacctcga
     2521 gacgtactgg tcgcgcatcc cgcgcacatg ggtgcacggt gactgggcga tcgtcgacga
     2581 agacggctac tggttcatcc agggccgatc cgatgacacg ctcaaggtcg cgggcaagcg
     2641 cgtcggcccg gccgaggtcg agagcgcagc ggtggcgcac ccggccgtcc tcgaggcggc
     2701 ggccgtgggc gtgccgcacg acatcaag
//
LOCUS       DATBRX010000035         4363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2468798, whole genome shotgun sequence.
ACCESSION   DATBRX010000035 DATBRX010000000
VERSION     DATBRX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2468798"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VIF08_01220"
     CDS             <1..1011
                     /locus_tag="VIF08_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134630.1"
                     /translation="VRESSYYYLLGYTSTKTGNDGKFHPITVRVKRGGVDVRARKGYW
                     ALTAADVEKINNPTPDVAKPVQTALASIATSVQSGKYVRTWIGTERGENGKTRVTLIW
                     EPLPAQGAAVGGSVRREQPGRVSLLAATAKGDLVFRGRSPDAALASAAPPVAPNDTGA
                     PAGRSPVAAAATAPQRLVFDAPPGTLELRMTVEAAGSGGTLDSEIRDITVPDFTAPQV
                     ALSTPRVYRARTAREFQALVADAAAVPQPGREFSRTERLLIRFDAYGPGSDKPAVTAV
                     LMNRAGQKMSDIQIAPAAAGAMHQIDLGLNSIPSGEYLVEITAKNGDGEVKELVPFRL
                     GS"
     gene            complement(1169..2242)
                     /locus_tag="VIF08_01225"
     CDS             complement(1169..2242)
                     /locus_tag="VIF08_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY8134631.1"
                     /translation="MRGPDEQTSHMFSYIVPEQRVRPDHPLRAIRRLTDEVLATLSPR
                     FDDMYSDMGRPSIPPEQLLRALVLQSLYTIRSERLLMEEIDYSILFRWFVGLGMDEEI
                     WSPTTFSKNRDRLLEGDVAAAFFDAVVAQARQAGLLSDEHFTVDGTLLEAWASLKSFR
                     PTEDDQAPPPDDPGNPTVNFHGETRRNDTHQSTTDPDAMLARKGQGKEAKLSYAGHVL
                     LDNRHGLVANVCVTAATGTAERDAALVMLAEAPAAVTVGGDKGFDVASFVAGVRALGV
                     TPHVAQKVTGSAIDERTTRHAGYAVSQQKRKLIEQVFGWLKTVGGLRKLRHRGGELVD
                     WIVTFAAAAYNLVRLRTLLARPA"
     gene            2433..3752
                     /locus_tag="VIF08_01230"
     CDS             2433..3752
                     /locus_tag="VIF08_01230"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY8134632.1"
                     /translation="MSRLPRSAPAWLDRLSAWLEPFEVCFTHVAQRGAFRRYLLGLLS
                     DSRRKSMSAMLERVRDPGTYQAFQHFITDAPWSADAVWRQLRATIPDRTGVLILDGTS
                     FPKQGPYSVGVARQYCGALGKIANCQTAVTVALWTGARAWMLGATLYLPDAWLTPEQR
                     TRARIPAGVRAQPKWQLALTLLRQVRASGITVTAVLGDAEFGESATLRRTLHRARLPY
                     ALGVSSTLKIFVGTPALVAPVSRGPIGRPRSRPTLAPDVSAIEARAWAAAHPARAWRV
                     VSWRNGTQPAWRARFCATRVTPAHEWRDRRLAPEVWLLCERALGASDRTKYYLVNVPA
                     TASLRTLVRLTHQRWAIEQQYQELKDELGLDHFEGRSLPGWQRHVVLTALAYSWLQSE
                     RHRRGAHLPTLPLVRAVITEILTAHFFVTHPHYLRTMQKLAEIDLRI"
     gene            3856..>4363
                     /locus_tag="VIF08_01235"
     CDS             3856..>4363
                     /locus_tag="VIF08_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEY8134633.1"
                     /translation="MRGPDEQTSQMFSYVVPEQRVRADHPLRAVRRLTDEVLATLSPR
                     FDRMYSEMGRPSIPPEQLLRALLLQSLYTIRSERLLMEEIDYSILFRWFVGLGMDDEI
                     WSPTTFSKNRDRLLQGDIAAAFFDAVVEQAREAGLLSDEHFTVDGTLLDAWASLKSFR
                     PKGEGPTAP"
BASE COUNT          706 a         1467 c         1449 g          741 t
ORIGIN      
        1 gtgcgcgaat cgagctacta ctacctgctt ggctacacct cgaccaagac cggcaacgac
       61 ggcaagttcc atccgatcac cgtccgcgtg aaacggggcg gcgtggatgt gcgcgcccgc
      121 aagggttatt gggcgttgac cgcggcggac gtcgagaaaa tcaacaaccc gacgcccgac
      181 gttgccaagc ccgttcagac cgcgctggcg tcgattgcga cctcggtaca gtcgggcaag
      241 tacgtgcgga cctggattgg gaccgagcgc ggagagaacg gcaagacgcg cgtgacgctg
      301 atctgggagc cgctgcctgc gcaaggcgcc gccgtgggag gcagtgtccg ccgcgaacag
      361 ccgggtcggg tgtcgctgct ggcggcaacg gccaaaggcg atctcgtttt ccgcgggcgc
      421 tcgccagacg ccgcgctggc gtctgccgcc ccaccagtgg cgcccaacga taccggggcc
      481 ccagccggcc gctcgccggt ggctgccgca gccaccgcgc cgcaacggtt ggtcttcgac
      541 gcgccgcccg gcacgcttga gttgcgcatg acagtcgagg ccgcgggatc cggcggcacg
      601 ctcgacagcg agattcgtga catcaccgtg cctgacttca cggcgccgca ggtcgcgctc
      661 agcacgccgc gtgtctaccg cgcacgcaca gcacgggagt tccaggccct ggtcgccgat
      721 gccgccgccg tgcctcagcc aggccgcgag ttctcacgca ccgagcggct gttgattcga
      781 ttcgacgcgt acggccccgg ctctgacaag cccgccgtga ctgccgtgct catgaaccgc
      841 gccgggcaga agatgtccga catccagatc gcgccagccg ccgccggcgc gatgcatcag
      901 atcgatctcg gtctcaactc gattccgtca ggagagtatc tcgtcgaaat caccgccaag
      961 aacggtgacg gcgaggtgaa agaactggtg cctttcagac tcgggtcata gtcaggtcaa
     1021 agggcaaagg gcaagagtca aaggttaggt actaggtact aggtactagc aggccgatga
     1081 aaaagtgact cgcgtcacca tgtcttctgt tcccgtgtgc gcccgccggt ggttgcggcg
     1141 gtacggttgc acgctcgtcg accggtgctc acgcgggcct cgccagcagc gtccgcaagc
     1201 ggaccaagtt gtaggccgcc gccgcgaagg tcacgatcca atcgacgagc tcgccaccgc
     1261 gatgccgaag tttgcgcagg ccgccgacgg tcttcagcca gccgaagact tgctcgatca
     1321 gtttccgttt ctgttggctg accgcgtagc cggcatgtcg tgtggtccgc tcgtcgatcg
     1381 cactgccggt gaccttctgc gcgacgtgcg gtgtgacgcc gagcgcgcgg acgccggcga
     1441 cgaaactggc gacatcgaag cccttgtcgc cgccgaccgt cacggccgcc ggggcttccg
     1501 cgagcatcac caaggcggcg tcccgttcgg cggtgcccgt ggcggcggtc acgcacacat
     1561 tcgccaccaa gccatggcgg ttgtcgagga gtacatgccc ggcgtaggag agcttcgcct
     1621 ccttgccctg gcctttgcgc gcgagcatcg cgtcgggatc ggtcgtcgac tgatgggtgt
     1681 cgttccgccg cgtctcgcca tggaaattca cggtgggatt tccgggatcg tcgggcggcg
     1741 gcgcctggtc gtcctcagtc gggcggaagc tcttgaggct cgcccacgcc tccagcagcg
     1801 tcccgtcgac ggtgaagtgc tcgtcggaga gcagcccggc ctgccgcgcc tgagcgacga
     1861 ccgcatcaaa gaatgccgcg gcgacgtcgc cctccagcaa gcggtcgcga ttcttactga
     1921 aggtggtcgg cgaccagatc tcctcgtcca tgcccaggcc gacgaaccag cgaaacagga
     1981 tgctgtagtc gatctcctcc atcagcagcc gctcgctgcg aatggtgtac agcgactgca
     2041 gcacgagcgc tcgcagcagc tgctccggcg ggatcgacgg ccggcccatg tccgagtaca
     2101 tgtcgtcaaa gcgcggcgac agcgtcgcga gcacctcgtc ggtcagccgg cggatcgcgc
     2161 gcagcggatg atccggccgc acgcgctgct ccggcacgat gtagctaaac atgtgactgg
     2221 tctgctcgtc aggtccacgc atgcggtcgc tccctctgaa cgaagtggga gcatggtagt
     2281 acgccgagat cacgatgtcg atcccccgcg agtcactttt tcagcaccct gctagtacta
     2341 ctttgtcaga tccactgatt catcccggaa tctgatccag acgcgacgaa aggtcttgta
     2401 catgtccgcg cggttcggta cgctgcggcg cagtgtcgcg actgccgcgg agtgcgccgg
     2461 cgtggctcga tcggttgagc gcctggctcg agccgttcga ggtgtgcttc acgcacgtgg
     2521 cccagcgcgg tgcgtttcgg cggtacctgc tgggcctgct gagcgacagc cgtcgcaagt
     2581 cgatgagtgc catgctggag cgcgtccgcg accccggcac gtatcaggcc tttcagcatt
     2641 tcatcaccga cgcgccgtgg tcggccgacg ctgtgtggcg ccagttgcgc gccacgattc
     2701 cggatcgcac gggcgtcctc attctcgacg gcacgagctt tcccaagcag gggccgtact
     2761 cggtcggggt cgcgcgccag tactgcggcg cgctcggcaa gatcgccaat tgccagaccg
     2821 cggtgaccgt ggcgttgtgg accggcgccc gcgcgtggat gctcggcgca acgttgtacc
     2881 tgccggacgc gtggctgacg ccggagcagc ggacgcgagc ccgcattccg gccggtgtgc
     2941 gtgcgcagcc gaagtggcaa ctcgcgctga cgttgctgcg gcaggtccgc gcgagcggga
     3001 tcacggtgac ggccgtcctc ggcgatgcgg aattcggcga gagtgcgaca ctgcggcgga
     3061 ccctccatcg ggcgcggctg ccgtacgcct tgggcgtctc gtccacgctg aagatcttcg
     3121 tcggcacgcc ggcgctcgtc gcgccggtgt cgcgcggccc catcggtcgt ccgcgatcgc
     3181 gcccgacgct ggcgcccgac gtctcggcga tcgaggcgcg cgcctgggcg gcagcccacc
     3241 ccgcccgtgc gtggcgcgtg gtgtcgtggc gcaatggcac gcagcccgcg tggcgcgccc
     3301 gcttctgtgc gacgcgtgtc actcccgcac acgagtggcg cgatcgtcgg ctcgcgcccg
     3361 aggtgtggct cctgtgcgaa cgcgcactcg gcgcgagcga ccgcaccaag tattacctgg
     3421 tcaacgtgcc ggcgaccgcg tcgttgcgca ccctggtgcg cctcacgcat cagcgctggg
     3481 cgatcgagca gcagtatcag gagctgaagg acgaactcgg cctcgatcac ttcgagggcc
     3541 gctcgttgcc cgggtggcag cgacacgtcg tcctcaccgc cctcgcgtac agctggctcc
     3601 aaagcgaacg ccaccgacgg ggcgcgcacc tgccgacgct cccactcgtg cgcgctgtga
     3661 tcaccgagat cctcactgcc cacttcttcg tgacgcaccc gcactatctc cgcaccatgc
     3721 agaaattggc cgaaattgac ctgcggatct aacaaagtcg tactagcagg gtgctgaaaa
     3781 agtgactcgc aggggatcga catcgtgatt tccgcgtact accatgctcc cacttcttcc
     3841 gaagggagcg accgcatgcg tggacccgac gagcaaacga gccaaatgtt cagttacgtc
     3901 gtgccggagc agcgcgtgcg ggcggaccat ccgctgcgcg ccgttcgccg cctgaccgac
     3961 gaggtcctgg ccaccctgtc gccgcgcttc gaccggatgt actcggagat gggccggccg
     4021 tcgatcccgc cggagcagtt gctgcgggcc ctgctgctgc aatcgctgta cacgattcgc
     4081 agcgagcggc tgctgatgga ggagatcgac tacagcatcc tgtttcgctg gttcgtcgga
     4141 ctcggcatgg acgatgagat ttggtcgccc acgaccttca gcaagaaccg tgatcgattg
     4201 ctgcagggcg acatcgcggc ggcctttttt gacgccgtcg tcgagcaggc gcgcgaggcc
     4261 gggctgctgt cggatgagca cttcacggtt gacggcacgc tgctcgacgc gtgggccagc
     4321 ctcaagagct tccggccgaa gggcgaaggg ccgaccgcgc cgc
//
LOCUS       DATBRX010000036         6829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1128374, whole genome shotgun sequence.
ACCESSION   DATBRX010000036 DATBRX010000000
VERSION     DATBRX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128374"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VIF08_01240"
     CDS             <1..409
                     /locus_tag="VIF08_01240"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEY8134634.1"
                     /translation="VADFRPRTQSTTKIARAGSGLTLELEPTQDILAEAVTTARAKAG
                     RQPVIVGFAAETGSLERAPAKAAKKGVDLMVANDVVHEGSGFGTDTNKVTIIEPNGPT
                     EEWPLMSKRQVADRLLDRMVELKMAKGAELAAR"
     gene            406..1365
                     /gene="panB"
                     /locus_tag="VIF08_01245"
     CDS             406..1365
                     /gene="panB"
                     /locus_tag="VIF08_01245"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY8134635.1"
                     /translation="MSATSQPQPAPAPGAKPVRRTTLSDIAKMHAEGTRIAMLTAYDY
                     PTARVIDEAGVPLILVGDSVGMVMLGYEETVRVSMDEMLHHTKAVVRGTRRSLVIGDM
                     PFLSYGVNIEQSLENAGRFISEAGAQAVKVEGGVRSARTIEAIAKAGIPVMGHIGLTP
                     QSVHGLGGHRVQGKTVESARHLLSDAFAVQEAGAFAVVLELVPAELSAAVTDRLKIPT
                     IGIGAGAGCSGQVQVITDLLGLTYGFIPKHARKYENLAERMAEAVGAYVADVTAGTFP
                     APENSSSIQPDVLAEVLGEGALDRAAATAAAAVGEPIPLDRDL"
     gene            1372..2217
                     /gene="panC"
                     /locus_tag="VIF08_01250"
     CDS             1372..2217
                     /gene="panC"
                     /locus_tag="VIF08_01250"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8134636.1"
                     /translation="MLVVRTRAELREARAALPGPLGFVPTMGALHEGHASLIRRARAE
                     CASVTASIFVNPTQFGANEDLTRYPRDEQGDLALLESLGVDLAFLPAVEEIYPPGASM
                     TVDVGRLGDVLEGTARPGHFRGVATVVTILFNLIAPNRAFFGQKDGQQSVVIRRLVRD
                     LGMPVDVVVCPTVREADGLAMSSRNRYLTSQERSQAPILNRALQAAAGALASGDRSAD
                     SLRQFMRYELAAASLGNVDYVSVADAETLQELDVLDRPALASLAVRFPSARLIDCQPL
                     EPPVS"
     gene            complement(2214..2825)
                     /locus_tag="VIF08_01255"
     CDS             complement(2214..2825)
                     /locus_tag="VIF08_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134637.1"
                     /translation="MLDHEEQFLFKEEILHLATAVADGGMLDNVEDLLTDVITSPRFD
                     SEVFTLERSLQVMLGARAGTTLVGLLTVTPDVFDQVAEVRIAPEIHERLVAWRARFGL
                     AIDNALNFLNNPDGIQGHNFSTQVAHVDDRRVLQGRLTLVRYNNEPQFFVADAAVFYS
                     LIADILDRLVPQTDDETVDPDTLSRIKDAIAVIERKQAARDRT"
     gene            complement(2837..3703)
                     /locus_tag="VIF08_01260"
     CDS             complement(2837..3703)
                     /locus_tag="VIF08_01260"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEY8134638.1"
                     /translation="MNAARFGAQSMTAPLREVLLRSPGPAFGRAFDDPAHGFLHPVDL
                     ELARRQHAALCELLDRLGVRVHVVDEDGLGPDSVYVFDPLLISDRGAIPLRSGKPTRR
                     GEEAALEAWTAQAAIPTIGHIAAPGTADGGDTFWLRPDLLVIGRSLRTNRSGADQLAA
                     IVRGRVEIVDMPYWHGPGEVLHLLSVISPVADDLAVVFPELLPASLHELITDLGVRTI
                     AVDEAEFETLGCNVLTVRPGVVIVAAGNPAVSAALADAGCEVHTFDAGEIGLNGGGGP
                     TCLTRPVLRTGL"
     gene            complement(3797..5062)
                     /locus_tag="VIF08_01265"
     CDS             complement(3797..5062)
                     /locus_tag="VIF08_01265"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY8134639.1"
                     /translation="MTTERAAPAINPWHVAQQQFDIAAEKLNLDPGLRQVLREPRREL
                     HVHFPVKMDDGSVKVFEGYRVQHNLGRGPAKGGIRYHQDVSIDEVKALAMWMTWKCAV
                     VGIPYGGGKGGVIVDPKKLSRRELENLSRRFFTEIEVLIGPEKDIPAPDVNTTPQVMA
                     WFMDTYSMHVGYTVPGVVTGKPISLGGSEGRNEATARGTVFCVLEAAKHLKLDIKTAT
                     VVVQGFGNAGSIAAQLMAAEGAKVLAVSDSTGGIHSPAGLDINRVVAWKAEHGTVVGF
                     PGAQAVSNAEILEIECDVLIPAALENQITAANAGKIKAKIVAEAANGPTTPEADEILF
                     KNGVFSIPDILCNAGGVTVSYFEWVQDLNRDHWSERVVNEKLHEIMVKAFGETLAISL
                     REGVHMRLAAYLLAVQRVADATSLRGLYP"
     gene            complement(5226..6071)
                     /locus_tag="VIF08_01270"
     CDS             complement(5226..6071)
                     /locus_tag="VIF08_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEY8134640.1"
                     /translation="MSRVAIVTDSASDLPAERAAAAGVIVVPLLVTFGEREFSAGVDM
                     SYEDFWRELTAPGAPFPKTAAAAPGAFKDAFDGLFAQGYDEIVCVNVGSKLSATMGSA
                     KIARAMLPDRAIHVFDSESATLGQGLLVLLAAQRAREGKSGAEIVAELERRKHTLSLY
                     LALETLEYLKRGGRISPARAAIGNVLSVKPIITIEDGVVETADKPRTRGKARARLLEL
                     LNTSSPEMIAVMHGQAEDIGEFADELAKLTKFPRDQVGIYLIGPSVGPHTGPGVYGAV
                     ILPKG"
     gene            complement(6090..>6829)
                     /locus_tag="VIF08_01275"
     CDS             complement(6090..>6829)
                     /locus_tag="VIF08_01275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY8134641.1"
                     /translation="GTHAERVAIKDAVSRADQDQFALESHQRAIAAINEGRFRDEIVA
                     VPVRKGKEEVLFDTDEGPRAETSLEALARLKPVFALPSGGDRGDATDGTVTAGNAPGI
                     TDGAAATVVASERVVERLGLKPLARIVGYSQAEVAPKWLFLAPVKGVQRLLERTGMAM
                     ADFDLVEINEAFAAQVLADGRELGFDWSRVNVNGGAIALGHPIGASGARIVATLLYEL
                     RRREGRYGLATLCLGGGGSVAMAFERV"
BASE COUNT         1093 a         2347 c         2283 g         1106 t
ORIGIN      
        1 cgtggccgac ttccggccgc gcacccaatc caccaccaag atcgcccgcg ctggctccgg
       61 actgacgctc gaactggagc caacgcagga catcctggcc gaggccgtaa cgacggcacg
      121 cgccaaggcc ggccgccaac cagtgatcgt cggcttcgcc gcggagaccg ggtcgcttga
      181 gcgcgccccg gccaaggccg cgaagaaggg cgtggacctg atggtcgcga acgacgttgt
      241 ccacgaaggc tctggctttg gcaccgacac caacaaggtc acgatcatcg agcccaacgg
      301 cccgaccgaa gagtggccgc ttatgtccaa gcgccaagtc gcggatcggc tgctcgatcg
      361 catggtcgaa ctgaagatgg ccaagggtgc agaattggcc gccagatgag cgccacgtca
      421 cagcctcagc cagcgcctgc gccgggcgcg aaaccggtcc gccgcaccac tctgtccgac
      481 atcgccaaga tgcacgccga gggcacacgc atcgcgatgc tgaccgcgta tgactacccg
      541 actgcgcggg tgatcgacga agcgggcgtg ccgctgatcc tggtgggcga ctcggtcggc
      601 atggtgatgc tcggctatga ggagacggtg cgcgtctcga tggacgagat gctccaccac
      661 accaaggccg tcgtgcgcgg gacgcgccgc tcgctggtca tcggtgacat gcccttcctg
      721 tcgtacggcg tgaacatcga gcagtcgctc gaaaacgccg ggcgcttcat cagcgaggcc
      781 ggcgcccagg cggtcaaggt cgagggcggc gtgcgcagcg cgcggacgat cgaggccatc
      841 gccaaggccg gcatcccggt catgggccac atcggcctga ccccgcagag cgtccatggc
      901 ctgggcggcc atcgcgtgca gggcaagacg gttgaatcgg cccgtcatct gctgtcggat
      961 gccttcgccg tccaggaggc gggcgccttt gccgtggtgc tggagctggt cccggctgag
     1021 ctgtccgccg cagtcaccga ccgactgaag atcccgacta tcggcattgg tgccggcgcc
     1081 ggctgctcag gccaggtgca ggtgataacc gatctgctcg ggctcaccta cggcttcata
     1141 cccaagcacg cacggaagta cgagaacctc gccgagcgca tggccgaggc ggtgggcgcc
     1201 tacgtcgccg acgtgacggc gggcactttc ccggcgccgg agaactcgtc gtcgatccag
     1261 cccgacgtgc tcgccgaggt cctgggtgaa ggcgcgctcg atcgcgctgc ggcaaccgcg
     1321 gccgcggccg ttggcgagcc catcccgctc gaccgcgacc tctaggtcct cttgctcgta
     1381 gtcaggacgc gcgccgagct gcgcgaggcg cgtgcagcac tccccggtcc gctcggcttc
     1441 gtgccgacga tgggcgcgct ccacgagggc cacgcctcgc tcatccgccg cgctcgtgcc
     1501 gaatgcgcgt cagtcacggc gagcatcttc gtcaatccca cccagttcgg cgcgaacgag
     1561 gacctgacgc gatacccgcg cgacgaacaa ggcgacctgg cgttgctcga gtcgctcggc
     1621 gtggacctcg cgttcctgcc cgcggtcgag gagatctatc cgcccggcgc atcgatgacc
     1681 gtggatgtcg ggcggctggg tgatgtgctg gaaggcaccg cgcggcccgg tcacttccgg
     1741 ggagtggcca ccgtggtcac catcctgttc aacctcatcg cgccgaatag ggccttcttt
     1801 ggccagaagg acggccagca gtcggtcgtg atccgccgcc tggtgcgcga cctcggcatg
     1861 cccgtagacg tcgtggtctg ccccaccgtc cgcgaggccg acggcctggc catgtcgtcg
     1921 cgcaaccgct acctgacatc gcaggagcgg agccaggcac cgatcctcaa ccgcgcgttg
     1981 caggccgcgg ccggcgcgct cgcttcgggc gatagatcgg ccgattcgct tcgtcagttc
     2041 atgcgctatg agctggctgc tgccagcctg ggcaatgtcg actacgtcag cgtggccgat
     2101 gccgagactc tgcaggagct cgatgtcctc gaccggccgg ccctagcttc gctggcagtg
     2161 cgatttcctt ccgcgcgact gatcgactgc cagccgctgg agccgcccgt tagctaggtg
     2221 cggtcgcgtg ccgcctgctt gcgctcgatg accgcgatcg cgtccttgat ccggctgagc
     2281 gtgtccggat cgaccgtctc gtcgtcggtc tgcggcacca gccgatccag gatgtcggcg
     2341 atgagcgagt aaaagactgc cgcgtccgcg acaaagaact gcggctcgtt gttgtagcgc
     2401 accagggtca ggcggccctg caggacgcgt cggtcgtcga cgtgggccac ctgggtggag
     2461 aagttgtgac cctggatgcc gtcggggttg ttcaggaaat tgagcgcgtt gtcgatcgcg
     2521 agaccaaagc gcgcccgcca cgcgacgagg cgctcatgga tctccggcgc gatgcgcacc
     2581 tccgcgacct ggtcgaagac atccggcgtc acggtcagca gcccgaccaa tgtcgtgccg
     2641 gcgcgcgcgc ccagcatcac ctgcaggctt cgctcgaggg tgaacacctc gctgtcaaat
     2701 cgcggcgatg tgatgacgtc ggtcagcagg tcctcgacgt tgtcgagcat cccgccgtct
     2761 gcgaccgcgg tcgcgaggtg gaggatctcc tctttgaaca ggaactgttc ttcgtggtcg
     2821 agcatgggcg ccgatgctac aaccccgtgc gcaggaccgg ccgcgtaaga cacgttggcc
     2881 cgccgccgcc gttcagcccg atctcgcccg cgtcgaaggt atgcacctcg cagccggcat
     2941 cggccagcgc ggcactgacc gcaggattgc ccgccgcaac gatgaccaca cccggccgga
     3001 cggtgagaac gttgcagccc agggtctcga actcggcctc gtcgaccgcg atcgtgcgca
     3061 cgcccaggtc ggtgatcagc tcatgcaggc tcgcgggcag aagctccggg aacacgaccg
     3121 ccaggtcatc cgcgaccggc gagatgacgc tcagcagatg caggacttcg cccggcccgt
     3181 gccagtacgg catgtcgacg atctccacac gaccgcgcac gatggcggcc agctggtcgg
     3241 cgccggaacg attggtgcgc agcgagcggc cgatgaccag caggtcgggc cgcagccaga
     3301 acgtgtcgcc gccgtcggcc gtcccgggag ccgcgatatg accgatcgtc gggatggccg
     3361 cctgcgccgt ccaggcttcc agcgccgctt cctcgccgcg gcgcgtcggt ttgcccgagc
     3421 gcaacgggat ggctccgcga tcacttatca gcaacgggtc gaagacgtat acggagtctg
     3481 ggcccagtcc atcctcgtcc acgacgtgga cgcgcacccc caggcggtcg agcagctcac
     3541 acagcgccgc gtgctgccgg cgcgcaagct cgagatcgac cgggtgcagg aagccgtgcg
     3601 ccgggtcgtc gaacgcccgc ccgaatgccg gaccgggcga gcgcaacagg acctcgcgca
     3661 atggagcggt catggattga gcgccgaacc gggccgcgtt catcggctca agtgtcgctg
     3721 gcgccgcaaa tagagaagcg ccccgcggac cttgcggccc cggggcgctc tgcgcgttac
     3781 gaggtgagag gtcgatttat ggatagaggc cgcgcaacga ggtcgcgtcc gccacccgct
     3841 ggacggccag caggtaggcg gcgaggcgca tgtgcacgcc ctcgcgcagc gagatcgcga
     3901 gggtctcgcc gaacgccttg accatgatct cgtgcagctt ctcgttgacg accctctcgc
     3961 tccagtggtc gcggttcagg tcctgaaccc actcgaagta cgaaacggtg acgccaccgg
     4021 cgttgcacag gatgtcgggg atcgagaaga cgccgttctt gaacaggatc tcgtcagcct
     4081 caggcgtcgt cgggccgttg gcggcctcgg caacgatctt ggccttgatc ttgcccgcgt
     4141 tggcggccgt gatctggttc tccagcgcgg ccgggatgag cacgtcgcac tcgatctcga
     4201 ggatctcggc attcgacaca gcctgggcgc cggggaaacc gacgaccgtg ccgtgctccg
     4261 ccttccaggc gacgacgcgg ttgatgtcca ggccggccgg cgagtggatc ccaccggtcg
     4321 aatcggacac cgcgagcacc ttggcgccct cagcggccat cagctgagcg gcaatggagc
     4381 cggcgttgcc aaagccctgg acgaccacgg tggccgtctt gatgtcgagc ttgaggtgct
     4441 tggccgcctc gaggacgcag aagaccgtcc cgcgcgcggt ggcctcattg cgaccctcgg
     4501 aaccgccgag cgagatcggc ttgccggtca cgacaccggg cacggtgtag ccgacgtgca
     4561 tggagtaggt atccatgaac caggccatca cctgcggcgt cgtgttgacg tccggtgcgg
     4621 ggatgtcctt ctcaggcccg atgaggacct cgatctccgt gaagaagcgc cggctcaggt
     4681 tctcgagctc gcgccgcgac agcttcttgg gatcgacgat cacgccgccc ttgccaccgc
     4741 cgtaggggat gcccacgacg gcgcacttcc aggtcatcca catcgcgagg gccttgacct
     4801 cgtcgatcga gacgtcctgg tggtagcgga tgccgccctt ggcagggccg cgacccaggt
     4861 tgtgctggac ccggtatccc tcaaacacct tgacggaccc gtcgtccatc ttgaccggga
     4921 agtggacatg caactcgcgt cggggctcac gcaggacctg gcgcaggcca gggtccagat
     4981 tgagcttctc ggcggcgatg tcgaactgct gctgcgccac gtgccatggg ttgatggccg
     5041 gcgctgctcg ttcggtggtc atcttgaccg aatcctccaa gggaacgatt gtcgttaggc
     5101 ccgagatggg ggccgaagcg ggcgaagttt acaccgggcc tcctgcccaa ccgatgtgcc
     5161 agaggtaacc gaagcgcgag ggccgtccgg cccacggatg gcccgccgcg cacgattggc
     5221 ggcgctcagc ccttgggcag gatgaccgct ccatagacgc cgggccccgt gtgcggaccc
     5281 accgacggtc cgatcaggta gatgccgacc tgatcacgcg ggaacttcgt cagcttggcc
     5341 agctcgtcgg caaactcccc gatgtcctcg gcctggccgt gcatgacggc gatcatctcc
     5401 ggcgacgagg tgttgagcag ctccagcagg cgggcgcgag ccttgcccct ggtgcgcggc
     5461 ttgtcggcgg tttcgacgac gccgtcttcg atcgtgatga tcggtttaac cgacagcaca
     5521 ttgccaattg ccgcgcgggc cgggctgatg cggccgccgc gcttgaggta ctcgagcgtc
     5581 tcgagcgcga ggtacaggct cagggtgtgc ttgcgccgct ccagctccgc gacgatctcg
     5641 gcgccggact tgccctcgcg cgctcgttgc gcggcgagca ggacgagcag gccctggcca
     5701 agcgtcgctg attcgctgtc gaacacgtga atcgcgcggt cgggcagcat ggcgcgcgcg
     5761 atcttggcgc tgcccatcgt ggcgctcagc ttcgaaccga cattgacgca cacgatctcg
     5821 tcgtagccct gggcgaatag cccgtcgaac gcgtctttga aggcgcccgg cgccgcggcg
     5881 gcagtcttgg gaaacggtgc gccgggcgcg gtcagctcgc gccagaagtc ttcgtatgac
     5941 atatcgacgc cggcgctgaa ctcgcgctcg ccgaaggtga cgagcagcgg caccacgatc
     6001 acgcccgccg ccgccgcgcg ctccgctggc aggtcgctgg ccgagtcggt gacgatcgcg
     6061 acccggctca tggcgttact cctgtgtccc tagacgcgct cgaaggccat cgccactgat
     6121 cccccgccgc ccaggcacag cgtcgcgagt ccatagcgtc cctctctcct gcgaagctca
     6181 tacagcaggg tagcgacgat ccgcgcaccg ctggcgccga tcgggtggcc aagcgcgatc
     6241 gcgccgccgt tgacgttgac cctgctccag tcaaacccca gctcgcgccc gtcggccagg
     6301 acctgggccg cgaacgcctc gttgatctcc acgagatcga agtcggccat cgccatgccg
     6361 gtccgctcca gcagcctctg gacacccttg accggcgcca ggaagagcca cttgggcgcg
     6421 acctcagcct ggctgtagcc gacgatccgg gcgagcggct tgagtccgag ccgctccacg
     6481 actcgttcgg acgcgaccac cgtcgccgcc gcgccatcag tgatgcccgg cgcgttaccc
     6541 gccgtgaccg tgccgtcggt cgcgtcgccg cggtccccgc ccgacggcag ggcgaagaca
     6601 ggcttcaggc gggcgagcgc ctcgaggctg gtctcggctc ggggtccctc gtcggtgtcg
     6661 aaaaggactt cttccttgcc cttccgtacg ggcaccgcca cgatctcgtc gcggaagcga
     6721 ccttcgttga tcgccgcgat tgcgcgctgg tgcgattcca gcgcgaattg atcctgatcc
     6781 gcccgactca cggcgtcctt gatggcgacg cgctcggcat gggtaccca
//
LOCUS       DATBRX010000037         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_570263, whole genome shotgun sequence.
ACCESSION   DATBRX010000037 DATBRX010000000
VERSION     DATBRX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570263"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1448
                     /locus_tag="VIF08_01280"
     CDS             135..1448
                     /locus_tag="VIF08_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8134642.1"
                     /translation="MPTRYVIVGNGPAGATAAQAIRERDATGDIHVIGAERHPFYSRP
                     GLAYYLTGQVPEGALFSRPDKEYKRAGIKKTTATVRAVRPHVVDLSDGRSLPYDRLLL
                     SVGAKAVRPDLPGIDLVGVVTLDNLDDARHIVKLARKAARAVVVGGGITAVELAEGLA
                     ANNVRVQYLLRGERYWSSVLADHESQLVEARLQADGISIRRNVELARVIGDRRNRVVG
                     VETKDGQLIACDMVAVAVGTQPRLELAQGAGVETGRGIFADEFLRTSQPDIYVAGDAA
                     EVLDPTTGRRGIDSLWSVANAQGRIAGANMAGDSRSFAPRPAPFNVTKIGGITTTVIG
                     AIGAGGRDGDLVTLARGDSNAWREQLEGFAIQDDVDADHLRLILSDKRILGAVVMGDQ
                     SLTRPLLHLIRSQVDIGAVYERFAAGGRAEIVAAVALLLGTPDAL"
     gene            1438..2100
                     /locus_tag="VIF08_01285"
     CDS             1438..2100
                     /locus_tag="VIF08_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134643.1"
                     /translation="MRYSELVIAAAIIALTTVGYATYVSTSGVPPAGDLVGHLLGIIG
                     FTLMLLTETLYSLRKRALGRARGSMRTWLRAHIVMGIVGPYLVLLHTAWEFNGLAGLL
                     TLMTAIVVISGFIGRYIYAAVPRTADGAVLEAQELNETLASLRLAEAARPGAMDEAAR
                     RARREIERRIRTLERQISALRWERRALATWHTIHIPLGMTMFVVAFAHIAAALYYGTL
                     MR"
     gene            2107..>2673
                     /locus_tag="VIF08_01290"
     CDS             2107..>2673
                     /locus_tag="VIF08_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY8134644.1"
                     /translation="MKNRPLGCFTFSALLFAFLAVVAVGAAMVLSGNSIFSPGDLSAL
                     TLDDGGQRVELGGVTSHSELGTRCDSCHTAIWSGVFMGDRCLACHTDVAQQTATRTGL
                     HGRLEVTAATCMGCHTDHRGATASATRADVNVFPHDDMGFALTAHLVPAVAQGIGCRE
                     CHQDSAKGDYERTDCLGCHQRLDQAKMAA"
BASE COUNT          427 a          911 c          906 g          429 t
ORIGIN      
        1 gtccgcacgg aagtgccaat tgcggaccgg gcctgcctca cctgtggcga gtgcgtcgcg
       61 cgctgcccgc gcggcaccct gcggtttgag caatcgtcgc tgttcaccgg ccgcgcggac
      121 ggggagcgct gagtatgccc acccgctacg tgatcgtggg caacgggccc gccggcgcta
      181 ccgctgcaca ggcgatccgc gaacgcgacg cgactggcga catccacgtc atcggcgctg
      241 agcggcaccc gttctactcg cgacctggcc tggcgtacta cctgaccggg caggtgccgg
      301 agggcgcgct gttctcgcgg ccagacaagg aatacaagcg cgcgggcatc aagaagacga
      361 cggccactgt ccgcgcggtc cgaccgcacg tagtcgacct gtccgacggt cgctccctgc
      421 cgtacgaccg cttgctgctg agtgtcggcg ccaaggccgt ccggcccgac ctgcctggaa
      481 tcgacctcgt gggcgtcgtc acgctcgaca acctcgacga tgcgcggcac atcgtgaagc
      541 tcgccagaaa ggcggcgcgg gcggtggtgg tgggcggcgg catcaccgcg gtcgagctgg
      601 cagagggtct cgccgcgaac aacgtgcgcg tccagtacct gttgcgcggc gagcgttact
      661 ggtcgagcgt cctggccgac cacgagtcac aactggtcga ggcgcgcctc caggccgacg
      721 gcatctccat tcggcgcaac gtcgagctgg cgcgcgtcat tggcgatcgg cgcaaccgcg
      781 tcgtcggcgt cgagaccaag gacggccagc tcatcgcctg cgacatggtc gcggtggccg
      841 tgggcactca gccgcggctc gagcttgccc agggagccgg cgttgaaacc gggcgcggca
      901 tcttcgccga tgagttcctc cggaccagtc agcccgacat ctacgtcgcc ggcgacgccg
      961 cggaggtgct ggaccccacg acgggtcggc ggggaatcga cagcctgtgg tcggtggcca
     1021 acgcccaggg ccgcatcgcc ggagccaata tggccggcga ctcacggtca ttcgcgccgc
     1081 gaccggcgcc gttcaacgtg accaagatcg gcggtatcac cacgacggtc atcggcgcca
     1141 tcggagccgg cgggcgcgat ggcgacctgg tgacgctggc ccgcggcgac agcaacgcgt
     1201 ggcgcgagca gctcgaaggc ttcgccatcc aggacgatgt cgacgcggat cacctgcgcc
     1261 tcatcctgag cgacaagcga atcctgggtg cggtggtcat gggcgaccag tccctgacac
     1321 gtccgctact ccatctcatc cgcagccagg tggacatcgg cgcggtctac gagcggttcg
     1381 cggcgggcgg tcgagccgag atcgtggccg cggtggccct gctgttaggg acgcctgatg
     1441 cgctatagcg agctcgtcat cgcggcagca atcatcgcgc taacgaccgt gggctatgcg
     1501 acgtacgtct ccacatcggg cgtaccgccg gcgggcgacc tcgtgggtca cctcctgggg
     1561 atcatcggct tcaccctgat gctcctgaca gagacgctct attcgttgcg caagcgagca
     1621 ctgggtagag cgcgcgggag catgcgcacc tggttgcggg cgcacatcgt gatgggcatc
     1681 gttgggccat acctcgtcct gcttcatacg gcctgggagt tcaacggcct ggcgggactc
     1741 ctgacgctga tgaccgcgat cgtggtgatc tccgggttca tcggccggta catctacgcc
     1801 gcggttccgc gaacagctga tggcgcggtg ctggaggcgc aggagctcaa cgagaccctg
     1861 gcctcgctgc gcctggcgga agcggcccgg cccggagcaa tggacgaggc ggcccgccgc
     1921 gctcggcgcg aaatcgaacg ccgcatccgg acactcgagc gccagatatc cgcgctgcgc
     1981 tgggagcgcc gcgcactcgc aacctggcac acgattcaca tcccgctggg catgaccatg
     2041 ttcgtggtcg cgttcgcgca catcgccgcc gccctgtact acggaaccct gatgcggtag
     2101 ccggcgatga agaaccgacc gctgggctgc ttcaccttct cggcgctgct gtttgcattc
     2161 ctggctgtcg tcgcagtcgg cgcggcaatg gtgctcagtg ggaactccat cttcagcccc
     2221 ggagatctgt ccgccctgac cctggatgac ggcggccagc gggtcgagct cggcggggtc
     2281 acgtcccact cggagctggg cacccgctgc gacagctgcc ataccgccat ctggtcgggt
     2341 gtattcatgg gcgacaggtg ccttgcgtgt cacaccgacg tcgcccagca gaccgcgacc
     2401 cgcaccggtc tccacggccg gctggaggtc accgcagcaa cctgcatggg ctgccacacc
     2461 gaccatcgcg gggcgacggc ttcggcaaca agggcggacg tgaacgtctt cccgcatgac
     2521 gatatgggtt tcgccttgac cgctcacctg gtgcccgcag tcgcgcaggg catcggttgc
     2581 cgcgagtgtc atcaggattc agccaagggt gattacgaac gcaccgattg cctcggctgt
     2641 caccagcgcc tcgatcaagc caagatggca gcc
//
LOCUS       DATBRX010000038         6650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1128877, whole genome shotgun sequence.
ACCESSION   DATBRX010000038 DATBRX010000000
VERSION     DATBRX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128877"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VIF08_01295"
     CDS             <1..417
                     /locus_tag="VIF08_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8134645.1"
                     /translation="ELVYPNGTRCHYLATGELTRGLFGLYRWESVPRGGGPGPHFHRT
                     FTESFYLLAGNIKIYNGREWIDAQPGDFVHVPEGGLHGFRNDSDSPATLLIHFAPGAP
                     REAYFEGNARFARDGRPDEATMADFYREHDNYFVAE"
     gene            complement(426..1103)
                     /locus_tag="VIF08_01300"
     CDS             complement(426..1103)
                     /locus_tag="VIF08_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEY8134646.1"
                     /translation="MSEFRGFKREAIQFLADLAANNDRAWFQPRKADYERLLKEPMEQ
                     LCVALSERFAARGIPLAADPRTSVFRIYRDTRFSKDKSPYKTHLAASFPLKGAKKQMH
                     GDGAYFHFQPGEMFMGGGMWRAEKPRLDAFRAAVLKKPDKVRAAFEDPAFVKVFKEVK
                     TEEALKRVPAGYPADHPMADMFRWKDVIFGKRLSDAQVLSAKLPDTLADSYAAALPVL
                     RFLATLG"
     gene            1234..1932
                     /locus_tag="VIF08_01305"
     CDS             1234..1932
                     /locus_tag="VIF08_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY8134647.1"
                     /translation="MSLTSAQPTAIAEPPVETLMAAAPRGLVPTPPDSRLSLLARRSF
                     RMLNHYFMVPALKAGLGPWMGTPFGGWILLLRVRGRKSGQLRDNPLSYLVADGAVWVM
                     AGFGSRTDWYRNLLADPKAEVILPGRTIACRAQSVEDADTRARIIPRLVRATGAPGYL
                     TGIDPFRSTDDQLLAVTAWVPLIRLVPEGAAVVAGPDDPGGLGWVWRQVVLLVIGALF
                     VRTVVRMLRGLRRR"
     gene            complement(1948..4653)
                     /locus_tag="VIF08_01310"
     CDS             complement(1948..4653)
                     /locus_tag="VIF08_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8134648.1"
                     /translation="MAALPTGTVTFLFTDIEGSTRLATELAERWPPILARHHELMREA
                     ISAHEGIEVMTEGDAFFVVFRAPAAAVSAAADAQRLLEQEAWPAGGEIHVRMGMHTGE
                     AVLGGDNYVGLDVHRAARVAAAGRGGQVLISESTRVLVDRALPEGVELKDLGSHRLKD
                     LPTPEHIYLLVIGGLQADFPPIRSLDARPNNLPMPVTTFVGRERQIEEIKSRLAVSRL
                     LTLTGPGGTGKTRLSIRVAEELLDEYRDGCWFVALDALREADLVPSAIASALGVRVAG
                     DRPALDALNAWLAEKQTLLVLDNFEQITAAAPLIGQMLAAAPGLRVLATSRIPLHIYG
                     ENEYAVPPLATVAELRAAAHNPEQLSTYEAVRLFIERALGAKSDFTVNNSNAPAVAEI
                     CARLDGLPLAIELAAARVKLLTPEQILARLESNLGVLATSAQDLPERQRTMRGAIEWS
                     YQLLTPDEQKTFARLSAFRGGFTLDSAEQVVLDKDLQIDIFDGLASLADKSLLRTLDA
                     GAETRFAMLETIREYATELLAAAPDADEIRRAHARHYFKLATDSESHLTAQDSGKWLD
                     GLEREHDNLRAAFSRAPELGLLDDALTAAGAIWRFWQQRGHFTEARTIYDRLLAQPGA
                     APAARAKALVGGGGIAYWQNDFTVVGPWYREAVDLYRTAGEERGTAEALYNLSYVPLL
                     EGDLPEAARIANEAVELFANLGDEAGVAKSQQVSAFVDYYSASPEASIELLEKASDIY
                     RRLGDQFLLADSLVSTSLPYANLGKWDDVGRLVNEGLDIFVRNNNQSGIAMVYEILGA
                     GLAYMDRDVEAAHMFGASENLRARIGGGAPTQLVNTEEYRKMAEAALGEMRFAELRTE
                     GSAMPDEAALAMAKGFKAAPGSPGLPPPAPWGKELERRKAADLID"
     gene            4886..5734
                     /locus_tag="VIF08_01315"
     CDS             4886..5734
                     /locus_tag="VIF08_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEY8134649.1"
                     /translation="MSVSLTGDPPVPPWLITHSYEDRDAGVLKSGKEAEVFLVERVAD
                     DGSSHLLAHKRYRPRYPKKGELEALGFSKGTLYRADAVYQTGWMLKPRERRALEAGSR
                     FGHELAKSMWPQIEKDTLSRAWQAGAAVPYPVELTDDGILMEYIGNRAGAAPRLAGAG
                     LSHEQARDARDQLFESLRALSAERIVHGDLSAYNMLWWEGRLVLIDFPQAVDAFANPH
                     APDLLHRDLRNVHEWFTRNRAEFDVEEVFVELVTLLYGGPAADKLTRRQASGRKIKHF
                     DSPQGR"
     gene            5792..>6650
                     /locus_tag="VIF08_01320"
     CDS             5792..>6650
                     /locus_tag="VIF08_01320"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY8134650.1"
                     /translation="MSLLRVIDDSGLVAPTMVAAFDAWVDAGSASTLAVGALIDSDAP
                     VVAELDADQLFDYRARRPTLDIIDGRPARLAWPTLVIRRTRLGERDLLVLTGPEPDFR
                     WRELADDVVAFARSVELEQWISIGAIPAAVPHTRPVPILGTESSPGLLRGDVVAGPRG
                     RLRVPSAALSMLDIGIAEAGIPALGYFAQIPHYVSGEYAPAAVALLEAVTGHLGVRVE
                     AVSLRAEAEQLRTRLNTATSLDEKTRSYVTRLEEMVDESGLPAGDELISEIERFLREQ
                     GNEPGHGQVH"
BASE COUNT         1024 a         2307 c         2234 g         1085 t
ORIGIN      
        1 gagctcgtct atcccaatgg cacgcgctgc cactacctcg ccaccggcga gctcacgcgc
       61 ggcctgttcg ggctctaccg ctgggagtcc gtgccgcgtg gcggtggccc cggtccgcac
      121 ttccatcgca ccttcaccga gtcgttctac ctgctcgccg ggaacatcaa gatctacaac
      181 ggacgcgaat ggatcgacgc ccagccgggt gatttcgtgc acgtgccgga gggtggcctg
      241 catggctttc gcaacgactc cgactcaccg gcgacgctgc tcatccattt cgcacctggc
      301 gcaccgcgcg aggcctattt cgagggcaat gcccgcttcg cacgcgacgg ccggcccgat
      361 gaagccacga tggccgactt ctaccgcgag cacgacaact acttcgtcgc cgagtgacgg
      421 ggcgatcaac cgagcgtcgc gagaaacctc agcacgggca aggccgcggc gtaggagtcg
      481 gcgagggtgt cgggcagctt cgcggatagc acctgcgcat ccgacaggcg cttgccgaat
      541 atgacgtcct tccagcggaa catgtcggcc atggggtgat cagccggata gccggccggc
      601 acgcgcttga gcgcctcctc cgtcttcacc tccttgaaga ccttgacgaa tgccgggtcc
      661 tcgaacgcgg cgcgaacctt gtccggcttc ttcagaacgg ccgcgcggaa cgcgtcaaga
      721 cgcggcttct cagcgcgcca catgccgccg cccatgaaca tctcgcccgg ctggaagtgg
      781 aagtacgcgc cgtcgccatg catctgcttc ttggcaccct tgagcgggaa cgacgcggcg
      841 aggtgcgtct tgtaaggcga cttgtccttt gagaatcgcg tgtcgcggta gatccggaag
      901 acggacgtcc tgggatccgc ggcgagagga attccgcgcg ccgcgaagcg ctctgacagc
      961 gcgacgcaga gctgctccat tggctccttc agcaaccgct cgtaatcggc cttgcgtggc
     1021 tggaaccatg cccgatcgtt gttggcggcg aggtcagcca ggaactggat cgcctcgcgc
     1081 ttgaagccgc ggaattcgct catggcattc accataacgg gcgcgactta tctgatgctt
     1141 acctgtgagc cgcgcgccgg ggagtgaaca ggcggccggg aaatatgccg accggcactt
     1201 gcccgcgctg cgcccacggc cgacgctcat gctgtgagcc tgacgagcgc gcagccgact
     1261 gcgatagccg agccaccggt cgagacattg atggcggccg cgccgcgcgg cctggtgccg
     1321 acgccgcccg acagcaggct cagcctgctg gcccgacggt ccttccggat gctcaaccac
     1381 tacttcatgg ttcccgccct caaggccgga ctcgggccct ggatgggtac gcccttcggg
     1441 ggctggatcc tgctgcttcg tgtgcgcggt cgaaagtccg gccagctgcg cgacaacccg
     1501 ctctcctacc tggtggccga cggcgcggtg tgggtcatgg ccgggttcgg cagccggacc
     1561 gactggtacc gcaacctgct ggccgatccc aaggccgagg tcatccttcc gggacgcacg
     1621 atcgcctgcc gcgcacagag cgttgaggat gcagacacgc gggcgcgcat catcccgcgc
     1681 ctcgtccggg cgacaggtgc gccgggctac ctgaccggca tcgatccctt ccgatcgacc
     1741 gacgaccagc tcctcgcggt caccgcctgg gtgccgctca tcaggctggt gccggaaggc
     1801 gcggcagtcg tcgcgggacc ggacgatccg ggcgggctgg gctgggtgtg gcggcaggtg
     1861 gtgctgctgg tgatcggtgc cctgttcgtc cgcaccgtcg ttcgcatgct gcgcgggttg
     1921 cgccggcgct agatcgcgcg gtcgcggtca gtcgatcagg tcggcggcct tgcggcgctc
     1981 gagctccttg ccccatggcg caggtggcgg caggcccgga ctacctgggg cagccttgaa
     2041 gcccttggcc attgcgagcg ctgcctcgtc gggcatcgcg ctgccctcgg tccggagctc
     2101 ggcgaaacgc atctcgccca gtgccgcctc tgccatcttg cggtactcct cggtatttac
     2161 gagctgcgtc ggcgcgccac caccaatgcg cgcgcgcagg ttctccgacg cgccgaacat
     2221 atgggcagcc tcgacgtcgc ggtccatgta ggccaacccg gcgcccagga tctcgtagac
     2281 catcgcgatg ccgctctggt tgttgttgcg cacgaagatg tccaggccct cgttcacaag
     2341 ccggccaacg tcgtcccact ttccgagatt ggcataaggc agcgaggtcg acaccagcga
     2401 gtcagcgagg aggaactggt cgccgaggcg gcggtagatg tccgacgcct tctcaagcag
     2461 ctctatcgat gcctcgggcg aagcgctgta gtaatcgacg aaggccgaga cctgctgcga
     2521 tttcgcgacg cccgcctcgt cgccgaggtt cgcaaagagc tcgacggcct cattcgcgat
     2581 ccgcgcggcc tctggcaagt cgccctcgag cagcggcaca taggacaggt tgtagagcgc
     2641 ctctgcggtg ccgcgctcct cgccggctgt gcggtacagg tcgacagcct cgcgatacca
     2701 cggcccgacc acggtgaaat cgttctgcca ataggcgatc ccgccgccac cgaccagcgc
     2761 cttggcgcgc gccgccggcg ccgcaccggg ctgcgccagc agccggtcgt agatggtccg
     2821 cgcctccgtg aagtggccgc gctgctgcca gaagcgccaa atggcgccgg cggcggtgag
     2881 cgcgtcgtcg agcaggccca gctccggggc gcggctgaac gcggcgcgca ggttgtcatg
     2941 ctcacgctcc aggccgtcga gccacttgcc cgaatcctgg gccgtgaggt gactctcgct
     3001 gtcagtcgcg agcttgaagt agtggcgcgc gtgggcgcgc ctgatctcgt cggcgtccgg
     3061 cgccgcggcg agcaactcgg tcgcgtattc gcggatcgtc tcgagcatcg caaagcgcgt
     3121 ctctgcgccg gcgtcgaggg tacgaagcag gctcttatcg gcaagggaag cgaggccatc
     3181 gaagatgtcg atctgcaggt ccttgtcgag caccacctgc tcggccgagt cgagcgtgaa
     3241 gccaccccgg aaagcggaca ggcgcgcgaa cgtcttctgc tcgtcgggcg tcagcagctg
     3301 gtagctccac tcgatcgcgc cgcgcatcgt gcgctgccgc tccggcaggt cctgggccga
     3361 cgtcgcgagc acgcccaggt tggattcgag ccgcgccagg atctgctccg gcgtgagcag
     3421 cttgacccgc gcggcggcca gctcgatggc cagcggcagg ccgtcgagcc gcgcgcagat
     3481 ctcggccacg gccggcgcgt ttgagttgtt cacggtgaag tcgctcttcg cgcccagggc
     3541 gcgctcgatg aagaggcgca cggcctcgta ggtcgagagc tgttcgggat tgtgtgcggc
     3601 cgcgcgcagc tcggccacgg tcgcgagtgg cggcaccgcg tactcgttct cgccatagat
     3661 gtggagcggg atgcggctcg tggccagcac gcgcagccca ggtgccgccg cgagcatctg
     3721 gccgatcagc ggcgcagctg cggtgatctg ctcgaagttg tcgagcacga gcagggtctg
     3781 cttttcggcc agccaggcgt tgagcgcgtc gagcgccggc cgatcgcccg cgacgcgaac
     3841 gcccagcgcg gacgcgatgg cgctcggcac gaggtcggcc tcgcgcaggg catcgagcgc
     3901 gacgaaccag cagccgtcgc ggtactcatc tagcagctcc tcggcgacgc ggatcgacag
     3961 tcgcgtcttg ccggttccac caggacccgt gagcgtcagg agccggctca ccgccaggcg
     4021 tgacttgatc tcttcgatct gccgctcacg ccccacgaac gtggtcaccg gcatgggcag
     4081 gttgttgggc cgggcgtcga gcgacctgat cggtgggaag tcagcctgta gaccaccgat
     4141 caccagcagg tagatgtgct caggcgtcgg gaggtccttc agccggtgac tgcccaggtc
     4201 cttgagctcg acgccctcag gcagcgcccg atcgaccagg acgcgggtcg actcggagat
     4261 gagcacctgg ccgccacggc ccgccgccgc gacgcgcgcc gcgcggtgga cgtcgaggcc
     4321 cacgtagttg tcgccgccca gcacggcctc accggtgtgc atgcccatac gcacatggat
     4381 ctcaccgccc gcgggccacg cctcctgctc cagcagccgc tgcgcgtccg cggccgcgct
     4441 cactgccgca gccggcgcgc gaaagacgac aaagaacgca tcgccctccg tcatcacctc
     4501 gataccttcg tgcgccgaga tcgcctcgcg catcagctca tggtggcgcg ccaggatggg
     4561 tggccagcgc tcggccagtt ccgtggcgag gcgcgtcgaa ccctcgatat ccgtgaagag
     4621 aaacgtgacc gtgccggtcg ggagcgcagc catcagtgcg cctattgtgg agtacactgc
     4681 gcaccgctca ccagccgcct tgacgccgca gacgaaagga gcagatgtcc gatgactgtc
     4741 tttgcccttt cgacctgccg tgcggaggct gactcgggcc tgtagccccc acgcgccctt
     4801 tagggcgcgc gctgcgtgac gcaaaacccc tttcgactcc gtgcgggcag ctgctttccc
     4861 ttggcctgct taccggagtt ttcgcttgtc tgtctcgctg acgggcgacc cgcccgtccc
     4921 gccatggctc ataacccact catatgaaga ccgcgacgcc ggcgtgctca agtcgggcaa
     4981 agaggccgag gtctttcttg tcgagcgcgt cgcggacgac ggctcgtcgc acctgctggc
     5041 gcacaaacgc taccggccgc gctaccccaa gaagggcgag ctcgaggcgc tcggcttctc
     5101 gaagggcacg ctctatcgcg cggacgcggt ttatcagacc ggctggatgc tcaagccgcg
     5161 cgagcggcgg gcactggagg ctggctcgcg gtttggccac gagctcgcca agagcatgtg
     5221 gccgcagatc gagaaggaca cgctctcacg cgcctggcag gccggcgcag ccgtgcccta
     5281 cccggtcgag ctgaccgacg acggcatcct catggagtac atcggcaatc gcgcgggcgc
     5341 cgcgccacgg ctggccggcg ccggactctc gcatgagcag gcgcgcgacg cgcgcgacca
     5401 gctcttcgag agcctgcgcg cgctcagtgc ggagcgcatc gtccacggcg acctgtccgc
     5461 gtacaacatg ctctggtggg agggccgatt ggttctcatc gacttccccc aagccgtgga
     5521 tgccttcgcc aatccacacg cgcccgacct gctgcaccgc gacctgcgca acgtgcacga
     5581 gtggttcacc cgcaacaggg ccgagtttga tgtcgaggag gtcttcgtcg agctggtgac
     5641 gctgctctat ggcggaccgg cggccgataa gctgacgcgc cgccaggcga gcggccgcaa
     5701 gatcaagcac ttcgacagcc cgcagggccg ctgacggcgc tggccggcat cgctgacggc
     5761 cgattcggcg cgccgctcct atgctgcgcc ggtgagcctg ctgcgagtga tcgatgacag
     5821 cggactggtg gcaccaacga tggtcgccgc cttcgacgca tgggttgacg cgggttccgc
     5881 ctcgacgcta gccgtggggg cgctgatcga ttcggacgcg ccggtggtgg ccgagctcga
     5941 cgccgatcag ctgttcgact atcgcgcccg ccggccgacg ctcgacatca tcgacggccg
     6001 cccggcgcgc ctcgcctggc caactctcgt cattcgccgg acgcgcctgg gcgagcgcga
     6061 cctgctcgtc ctgaccgggc cggagcctga ctttcgctgg cgcgagctgg ccgacgacgt
     6121 ggtcgcgttc gcgcgctcgg tcgagctcga gcagtggata tcgattggcg cgatcccggc
     6181 tgccgtgccg cacacccggc ccgtgcccat cctgggcacc gagtcatcgc ctggtctgtt
     6241 gcgcggcgat gtcgtcgcag gtccgcgcgg ccggctgcgc gtcccatccg cggcgctcag
     6301 catgctcgac atcgggatcg ccgaggcggg cattccggca ctgggctact tcgcgcagat
     6361 cccgcactac gtgagcggcg aatacgcgcc cgcagccgtg gccctgctcg aggcggtcac
     6421 cggccacctc ggcgttcgcg tcgaggcagt ctcgttgcgc gccgaggccg agcagctgcg
     6481 cacccgcctc aataccgcga catcgctcga cgagaagacg cgctcatacg tcacgcgcct
     6541 cgaggagatg gtcgacgagt cgggattacc ggcgggcgac gagctgatca gcgagatcga
     6601 gcgcttcctg cgcgaacagg gcaacgaacc gggccacggc caggtccact
//
LOCUS       DATBRX010000039         6329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1352794, whole genome shotgun sequence.
ACCESSION   DATBRX010000039 DATBRX010000000
VERSION     DATBRX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352794"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VIF08_01325"
     CDS             complement(<1..514)
                     /locus_tag="VIF08_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134651.1"
                     /translation="MNNRFLRNGIVTLILVVGTAALLYMFIFPTDGNKPIAYSGPNGS
                     FLQLVADGQVSKVTQRGQVLEIEKTTIDQHTGKPEIVTSNVPSELATNVQTDINAACS
                     ASPKCAAAPPTLTGAPPSDSAWLSILISAFLPILLIGALLFFMFRQAQGSNNQALSFG
                     KSRARMFLGNK"
     gene            complement(667..741)
                     /locus_tag="VIF08_01330"
     tRNA            complement(667..741)
                     /locus_tag="VIF08_01330"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(707..709),aa:Met,seq:cat)
     gene            complement(812..1087)
                     /locus_tag="VIF08_01335"
     CDS             complement(812..1087)
                     /locus_tag="VIF08_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8134652.1"
                     /translation="MLNIIAWIVLGAIAGYIAGMLVKGDERWGIIGHIVLGIVGAFVG
                     GFIALQLGLEPGAAFGGDKLSIVSIIVAVIGAVIVVAVVNMLTRRRV"
     gene            complement(1211..1705)
                     /locus_tag="VIF08_01340"
     CDS             complement(1211..1705)
                     /locus_tag="VIF08_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY8134653.1"
                     /translation="MSTDRLNRLLAANAAFARRFERGHLTAPPLERLAVLTCMDARIA
                     VEELFGLNVGDVAVVRNAGAVASDDALRSLLVTRHVLGTDEIVVMAHTGCGLRGLEDT
                     SLRERLTSEFGQPSATTFGSFDDLETHVREQVARIRAHPWLGDAPVHGLVYEVETGRA
                     RPVA"
     gene            complement(1880..2893)
                     /locus_tag="VIF08_01345"
     CDS             complement(1880..2893)
                     /locus_tag="VIF08_01345"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY8134654.1"
                     /translation="MVLPPRSSWPPPFAALSPDRRTWRRRAVRDGLIISGWLATGFAL
                     IVVTAIVHSVGYDAYSYWSIDFADLYGRAMGSNFALGAFRYAPPIAFLFGAFGALPWW
                     LFLWLWEALMLALVAWLGGRWALALLALPPVALELYHGNVHLLIAAAIVLGLRYPAAW
                     SFVLLTKITPGVGLLWFAARREWRSLAIALGATVAVSAAAFVVAPHYWREWLESILSN
                     LNEPQSYSLPPPAPIRLPIAALIVIWGARTDRPWTVAVAATLGLPIIWPHGLCVLAAA
                     IPFLRRGDRAAQAPDWLSAVQPRKLVLSVAIFVGGALILGLAFASPISQLMEGASANF
                     YGS"
     gene            2927..3892
                     /locus_tag="VIF08_01350"
     CDS             2927..3892
                     /locus_tag="VIF08_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134655.1"
                     /translation="MPMDNLPPLPPPPDPGQPGHNPTQRGFYPLDLDAITRNAWSIFR
                     FAWPTFIGAALIPNLIVYAIAIPVHASIWPEVSQWAAAYRAAIERGVLPTLPPIAPST
                     VALLVLVSILGALGGLVASAAVVSIGDSVFRGRPASIRGALRTALRRLPALIGAGLLY
                     ALAVFGVSLLGFTFGALFMVGGGVAAFLGLVVIVAAVAAVIFIALRWSLLVQTIVLEG
                     VGPVDGLSRSWRLVAGSGWRVLGYFLFIALVSGVIGAIVGAIPDAILRLSPESVTSAV
                     VGIVFQGVATVLVAPIVLVSLFLYYDLRFRHGEPAPQPGEDRRAA"
     gene            complement(3908..4402)
                     /locus_tag="VIF08_01355"
     CDS             complement(3908..4402)
                     /locus_tag="VIF08_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134656.1"
                     /translation="MPKPAVAVASATGASERVQTGPDTELNARLTTLGRQIGYGRGLR
                     PGRAQLLALGILIVAFWLVVTFGRALGQLNDATQRQHSAAAEAAALQLQLEAGRRELA
                     LVQTDAFQRLQARALGMGEMGELVFTLTSDTPPPAIVPLGQSASGSVEKGPLEAWLEL
                     LFGR"
     gene            complement(4458..>6329)
                     /locus_tag="VIF08_01360"
     CDS             complement(4458..>6329)
                     /locus_tag="VIF08_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8134657.1"
                     /translation="NEALIVAGSRGSKVRDERGEVAAASAQAADKGIRVVVGGGTFVL
                     PLIHKVGRLKLTARQIAVALPDAVTSQGIKVAVQGVATFKIGRDVESIRNAAERFLDS
                     RDEQVDSIVKNVLEGSLRSIVGTLTIEELIMDRQKLLQQVQDSAKGDLATSGLQIDAF
                     TIQSINDESGYVDLLGQQKLAIVERDARMAKATADQEAAVREAEANQVKLNAERDVEL
                     RRADVLTQTAAAQAKAGQAGPLAQAEAQQEVTRKQTELAELEAARKQKELLTSTIRPA
                     EAEAEAVVRRAEGDKAAKIAAAQAEAEREKLAGQAQASVIQVKGEANARVTLVNAEAE
                     SKRVTLEGNAEAGITFTKGEAEAKALALRAEAFQKFNEAAVIQTVLGMLPEIVRAAAE
                     PMSQIDHLTVLSNDGASELVRNTTRTVTEASATVKGLTGLDVPALISSAMGRATNGSP
                     KSDPTPPGGGAASKQGRRPAPRNPAPPAAAVGSEPIETVEGADAAIARASEAMKRASA
                     RAAAPAAGGTMPAVPPLHNASAKSANGSPTAPDRSTIPPITADMSAAEAANILAEQLR
                     RIPGIERNLAIPLVELDQRGPRAVRAAWQLAKPRIAARYGDMTVGELITRFGPTLNA"
BASE COUNT          961 a         2160 c         2151 g         1057 t
ORIGIN      
        1 tcttgttgcc caggaacatc cgcgcccggc tcttgccgaa gctgagtgcc tggttgttcg
       61 agccctgcgc ctggcggaac atgaagaaga gcagggcacc gatcaacagg atcggcagga
      121 aggcactgat gaggattgac agccacgcac tgtcagatgg cggagcgccg gtgagcgtgg
      181 gtggtgcggc ggcgcacttg ggcgacgccg agcaggcggc gttgatgtcg gtctggacat
      241 tggtcgcgag ctcgctggga acattgctcg tcacgatctc gggcttgccg gtgtgctggt
      301 cgatcgtggt cttctcgatc tcgagcacct gcccgcgctg ggtgaccttg gacacctggc
      361 cgtcggcaac gagctgcagg aaggagccgt ttggcccgct ataggcgatt ggcttattgc
      421 catcggtcgg gaagatgaac atgtaaagca gcgcggcagt gccgaccacg aggatcagcg
      481 tgacgatgcc gttgcgcagg aaccggttgt tcaaaaggga cgggcctccg agtggccggg
      541 cgcacctggc gccgggtagg gaattatagg ctggagcacc tgtgcgcccc tcacagccaa
      601 cccggcatga gttacgacgc tacgaccaga tgacgaaggt ggtacgcgcg gtgtgccggc
      661 atggattggt agcggggggc ggatttgaac caccgaccaa gggcttatga gtcccctgct
      721 ctaccactga gctaccccgc cgcggtcggc cgattatcgc gcacttgcgc ctcggccggc
      781 ctacggcggg ttgctcgtca gcccctgagt gtcagacgcg gcgccgggtc agcatgttta
      841 cgaccgccac cacgatgacg gcgccgataa cggcaacaat gatggaaacg atgctgagct
      901 tgtccccgcc gaacgcggca ccgggttcga gacccagctg cagggcgatg aagccgccaa
      961 cgaaggcgcc gacgataccc agcacgatgt gtccgatgat gccccagcgc tcgtcaccct
     1021 tgacaagcat tccggcgatg tagccggcga ttgcgcccag gacgatccag gcaatgatgt
     1081 ttagcacagg tttgacctcc ctaccttgcc gggaactctg gcccggtcgg ccggactatg
     1141 cggcccggac cgtgctggtc caatctaccg gaccgtaggg cgcattgcga tcggctagcg
     1201 aaaacggggg ctacgcgact gggcgcgccc ggccggtctc cacctcgtag acgagtccgt
     1261 ggacaggcgc gtcgcccagc cacgggtggg cgcggatgcg cgcgacctgc tcgcggacat
     1321 gcgtctccag gtcgtcgaac gacccaaagg ttgtcgcgct gggctgaccg aactcgctgg
     1381 tgagccgctc gcgcagcgac gtgtcctcga ggccgcgcag gccgcagccg gtgtgggcca
     1441 tgacgacgat ctcgtccgtg cctagtacgt ggcgcgtgac cagcagcgag cggagggcgt
     1501 catcgctcgc caccgcgccg gcgttgcgga cgacggccac gtcgcccacg ttcaggccga
     1561 acagctcctc tacggcaatg cgcgcgtcca tgcacgtcag gacggccagc cgctcgagcg
     1621 gtggcgccgt caagtggccc cgctcgaagc gtcgtgcgaa ggcggcgttg gcggccagca
     1681 gccggttcag tcggtcggtg ctcacgcggc cgccgattgt acggacagcc cccgctcatg
     1741 gccccggtga ttgcattaca ccttcatttc agcgcatccc ggaggcgcgg gcccttggac
     1801 gcaacgtgca gacctgatcc caggagccgg gcagcagcaa tgcccgactt ctgctgttag
     1861 cgcgctggat cggcatccat caggagccgt agaagttcgc cgaggcccct tccatcagct
     1921 ggctgatcgg gctcgcgaag gcgagcccaa ggatcagcgc accgcccacg aagatcgcga
     1981 ctgacaggac gagcttgcgc ggctgtacgg cgctgagcca gtcgggcgcc tgcgcggcgc
     2041 ggtcgccgcg gcgcaggaac gggatggccg ccgcgagcac gcacaggccg tgcggccaga
     2101 tgatcggcag gcccagggtt gcggcgaccg cgacggtcca cgggcggtcg gtgcgcgcac
     2161 cccagatgac gatgagcgcg gcgatcggca ggcgaatggg cgcaggcggc gggagggagt
     2221 aggactgcgg ctcgttgagg ttgctcaaga tcgactcgag ccactccctc cagtagtgcg
     2281 gcgcgacgac gaacgcggcc gcgctgaccg cgaccgtcgc gcccagcgcg atggccagcg
     2341 agcgccactc gcggcgcgcg gcgaaccaga gcaggcccac gcccggcgtg atcttggtca
     2401 gcagcacgaa cgaccatgcg gccgggtagc gcaggcccag cacgatcgcg gccgcgatca
     2461 gtagatgaac gttcccgtgg tacagctcga gggccaccgg cggcagcgcc aggagagcca
     2521 gcgcccagcg cccgcccagc cacgcgacga gcgccagcat cagtgcttcc cagagccaca
     2581 ggaaaagcca ccacggcagc gccccaaacg ccccgaagag gaacgcgatc ggcggcgcat
     2641 agcggaacgc gcccaaggcg aagttgctgc ccatcgcgcg gccgtacagg tccgcgaagt
     2701 cgatcgacca gtacgagtac gcgtcgtagc caacgctgtg cacgatggcg gtgacgacaa
     2761 tcagcgcgaa tccggtggcc agccagccgc tgatgatcaa tccgtcgcgc accgcccgac
     2821 ggcgccacgt cctgcgatcc ggagacagcg ccgcgaatgg tggcggccag ctcgaccgcg
     2881 gcgggagcac catggtgcga ggctacccga cacctaaact gccgggatgc cgatggacaa
     2941 cctgccgccg ctgccgccgc cgcccgatcc ggggcagccg ggccacaacc cgactcagcg
     3001 cgggttctac ccactcgacc tcgacgccat cacgcgcaac gcctggagca tcttcaggtt
     3061 cgcctggcca accttcatcg gcgcggcgct gattcccaac ctcatcgtct atgcgatcgc
     3121 catacctgtc cacgcctcga tctggccgga agtcagccaa tgggccgcgg cttacagggc
     3181 ggcgatcgaa cgcggcgtgc ttccgaccct cccgcccatc gctccatcta ccgtcgcgct
     3241 gctggtgctg gtgagcatcc tcggcgcgct cggcggactc gtggccagcg ctgccgtggt
     3301 gagcatcggc gactcggtct tccgtggccg cccggccagc attcgcggtg ctctcaggac
     3361 cgccctgcgc agactgccgg cgctcatcgg ggcaggtctg ctctacgcgc tcgctgtctt
     3421 cggcgtttca ctgctcggct tcacgtttgg tgccctattc atggtgggcg gtggtgtcgc
     3481 cgctttcctt ggcctggtgg tcatcgtcgc ggccgtggcc gcggtcatat tcatcgccct
     3541 gcgctggtct ctcctggtcc agaccatcgt cctcgaggga gtcggacccg tcgacgggct
     3601 ttcgcgttca tggcggctcg tagccggttc gggctggcgc gtgctgggct acttcctgtt
     3661 catcgccctg gtcagcgggg tgatcggagc catcgtgggg gccattcccg acgccatcct
     3721 gcggttgtcg cccgaatcag tgaccagtgc cgtggtgggc atcgtcttcc agggcgtcgc
     3781 gacggtgctc gttgcaccca tcgtgctggt gtcgctgttc ctgtactacg acctgcgctt
     3841 cagacacggt gagcccgcac cacagccagg tgaagatcgc cgcgccgcct aacggccgcg
     3901 gcgcggacta acggccgaac agcagctcca accaggcctc gaggggcccc ttctccaccg
     3961 acccggaggc gctctggccc agcggcacga tcgccggtgg tggggtgtcg ctcgtcaggg
     4021 tgaagacgag ttcgcccatc tcgcccatgc ccagagctcg cgcctggagc cgctggaacg
     4081 cgtcagtctg aacaagcgcc agctcgcgcc gcccggcctc gagctgcagc tgcagcgccg
     4141 ctgcctccgc cgcggctgag tgctggcgct gtgtcgcgtc attgagctgg cccagggcgc
     4201 gaccgaacgt cacgaccagc cagaaggcga cgatcaggat gcccagtgcg agcagctggg
     4261 cgcggcccgg tcggaggccg cggccatagc cgatctggcg gccgagcgtg gtcagccgcg
     4321 cgttgagctc tgtgtctggg ccggtctgaa cgcgctcgga ggcgcctgtc gcgctcgcca
     4381 cggccacggc tggtttcggc attcgaatca gtatgcccgc ctcgaatcgt gcgtgtgcgg
     4441 cgctgccgca ctacgtctca ggcgttgagc gtcgggccga agcgcgtgat gagctcgccc
     4501 accgtcatgt cgccatagcg cgccgctatc cgtggcttgg ccagctgcca cgcggcacgc
     4561 acggcgcgcg gcccacgctg gtcgagctcg accagcggta tggccaggtt gcgctcaatg
     4621 cccggtatgc gacgcagctg ctcggcaagg atgttggcgg cctcggctgc ggacatgtcg
     4681 gccgttatcg gcggaatggt cgaccgatcg ggagctgttg ggctgccgtt ggcagacttg
     4741 gcgctcgcgt tgtgcagcgg tggaacggcc ggcatcgtgc caccggcagc cggcgcagcg
     4801 gctcgtgctg aggcgcgttt catcgcttca gatgcccgcg caatcgccgc gtccgccccc
     4861 tcgaccgtct cgatgggctc ggatcccacc gcggcagccg gtggcgcggg attacgtggt
     4921 gcggggcggc ggccctgctt ggacgctgcg ccgccaccgg gcggcgtggg gtcgctcttt
     4981 ggactgccat tcgtggcgcg tcccatggca cttgagatca gcgccggcac gtcgagtccg
     5041 gtcaggccct taaccgtcgc actcgcctcc gtgaccgtgc gcgttgtgtt gcgcaccagc
     5101 tcggaagcgc cgtcgttgga cagaacggtc aagtggtcaa tctgcgacat gggctcggcc
     5161 gccgcacgca cgatctcggg cagcatgccc aggaccgtct ggatgacagc cgcctcgttg
     5221 aacttctgga acgcctcggc gcggagcgcg agcgccttgg cctcagcctc gcccttggtg
     5281 aacgtgatgc cggcctcggc attgccctcg agggtcacgc gcttcgactc tgcctccgca
     5341 ttgaccagcg tgacccgggc attggcctcg cccttgacct ggatgactga tgcctgggcc
     5401 tggccggcca gcttctcgcg ctcggcctct gcctgcgcgg cggcgatctt cgccgccttg
     5461 tcgccttcgg cccgcctgac cacggcctcc gcctctgcct cggccggccg gatggtcgag
     5521 gtgagcagct ccttctgctt gcgcgcagcc tcgagctcgg ccagctctgt ctgcttgcgg
     5581 gtgacctcct gctgtgcctc tgcctgggcg agaggtccgg cctgaccggc cttcgcttgg
     5641 gccgcggcgg tctgcgtgag cacgtcggca cggcgcaact cgacatcgcg ctcggcgttg
     5701 agcttgacct ggttggcttc cgcctcgcgg acggctgcct cctgatcggc tgtcgccttc
     5761 gccatgcggg catcgcgttc gacgatcgcc agcttctgct gtccgaggag gtcgacatag
     5821 cccgactcgt cgttgatgga ctggatcgtg aaggcatcga tctgcagccc ggatgtcgcc
     5881 agatcgccct tggccgagtc ctggacctgc tgcaggagct tctgccggtc catgatcagt
     5941 tcctcgatag tgagcgtgcc cacgatcgag cgcagcgaac cctccagcac gttcttgacg
     6001 atggagtcca cctgctcgtc gcgcgaatcc aggaagcgct cggcggcatt gcggatcgac
     6061 tcgacgtcgc ggccgatctt gaacgtggcg acgccctgca cggccacctt gatgccctgg
     6121 ctggtaaccg cgtcgggcaa cgcgacggcg atctggcgcg cggtcagctt gagccggccg
     6181 accttgtgga tcagcggcag cacgaatgtg ccgccgccca cgacgacgcg gatgcccttg
     6241 tccgccgcct gggcagacgc cgccgcgacc tcgccgcgtt catcacggac cttggagccg
     6301 cgcgatccgg cgacgatcag cgcctcgtt
//
LOCUS       DATBRX010000040         6652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_571016, whole genome shotgun sequence.
ACCESSION   DATBRX010000040 DATBRX010000000
VERSION     DATBRX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571016"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..922)
                     /gene="tpiA"
                     /locus_tag="VIF08_01365"
     CDS             complement(110..922)
                     /gene="tpiA"
                     /locus_tag="VIF08_01365"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY8134658.1"
                     /translation="MSTRAARTQPRPVIVAGNWKMNTTPLDAALLANDIAAATEVEGV
                     IRVICPPFVCLQAVSSALQDTSVSVGVQNIHAEPFGAYTGEISAPMIAGLASWAIIGH
                     SERRRDQAEDDVLIGRKLLRCAEFGLRPILCVGEQIEQRRAGKAEQTVRRQLERTLAE
                     IARASGEIPSDLVIAYEPVWAIGTGLTARGSDASAMAQLIRETLAGAGMPGAAAHVPI
                     LYGGSVTSAAIDEFLAEPEIDGALVGGASLKVDEMAGIVARAGITAAARQSA"
     gene            complement(919..1683)
                     /gene="whiA"
                     /locus_tag="VIF08_01370"
     CDS             complement(919..1683)
                     /gene="whiA"
                     /locus_tag="VIF08_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEY8134659.1"
                     /translation="MSAATPDLATALRAELAAVDPARRCCRAAERDGLGAAALGRART
                     PLLGRLAVRLDDAAGEGSFDWASAPEHDRVAWLRGALLAHGSLSVTPTGTHLELTTGP
                     QDLEPLGEWLAAHGYPAGARVRRGRGVLTWKSAETIVALLRRLGASAVTLELETRLIG
                     RSVAGQLNRVVNAEHANLRRAVGAARRQLAEIDALERQGTLRRLPRQARLVARARRRA
                     PEATFSQLAADLDLSRGQVQRAFGLIESAALHEGAE"
     gene            complement(1680..3074)
                     /gene="yvcK"
                     /locus_tag="VIF08_01375"
     CDS             complement(1680..3074)
                     /gene="yvcK"
                     /locus_tag="VIF08_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HEY8134660.1"
                     /translation="MNGAGPSADPSHDARRLEAALRRRERAQVLRQWLRPGVGVKRWL
                     VLGFIGLFLVALAVAALLRTALADRSADDPLHDIIEILTLQSLPPELRWMLPLGLGLI
                     LFIYSSWRLLRALVEPYRSAQEPLAELLYQRRLRARGPRVVAIGGGTGLSALLRGLKE
                     LTSNITAVVTVADDGGSSGKLRTELGMPPMGDIRNCIAALADTEPQMARLLSYRFPLA
                     DSVDSSYAGHAFGNLLIAALTAVSGDFEEGVRQSNRVLAVRGSVVPVAGQPITLHAAL
                     IDGSVMEGQSEIARKRGIRRVWISPEDVKPSAEALEAIAAADLIIVGPGSLYTSLLPP
                     LLVPGIHDALTRASAPRLLVCNVATQVGETEEFALSDHLDALGGHGMADVIDAVLVND
                     NFRARQPHNYPAAPVRIDVPLSSKTGPQIIARDVVDDDNAHRHDSRKLASVLLTLYDE
                     RAIARRARATTKAR"
     gene            complement(3071..3952)
                     /gene="rapZ"
                     /locus_tag="VIF08_01380"
     CDS             complement(3071..3952)
                     /gene="rapZ"
                     /locus_tag="VIF08_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY8134661.1"
                     /translation="MAKHTKSHVAIISGLSGAGKTAASKLLEDLGYRVVDNLPAELLR
                     NLAQLVAREPERYERVALVLDARAGDVADAFHQAMDALESRGITPQVFFLEARDDVLI
                     RRFSETRHRHPLDTAERGVAASIARERELLAEIRAEADVIVDTSDMSGRQLRERLTST
                     LSTPTGREGITLQLVSFAYKHGIPLEADVVFDVRFMENPFYIDRLRPLNGLDPQVRDF
                     VLDQPLTPRFLDEVMRFLELTLPGYESEGRSRLTVAFGCTGGQHRSIAIAEEVAARLR
                     REKGGPVSVWHRELERS"
     gene            complement(3945..4709)
                     /gene="tatC"
                     /locus_tag="VIF08_01385"
     CDS             complement(3945..4709)
                     /gene="tatC"
                     /locus_tag="VIF08_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8134662.1"
                     /translation="MTETVVTDHPPDSGDGKIMTVVEHLSELRRRIIVSFAVIAIGAA
                     VGFWLAPDAIRFLKTPISQPLYFTAPSSAFFIQLKLALMIGIALASPILLYQLWAFIA
                     PGLTPRERRLALPWVPLGLVFLALGVSVAWFILPFAVGFMLGFAIPGVMDPLITADAY
                     FGFVTNLFLAFGLVMEFPIVLVLLSKVGIVSAERLRRNRRYVFLAIFVFAVVVTPGGD
                     PYSPTVMGLVMYILYELTIRLVARGDKSRAEEGAGG"
     gene            complement(4746..4994)
                     /gene="tatA"
                     /locus_tag="VIF08_01390"
     CDS             complement(4746..4994)
                     /gene="tatA"
                     /locus_tag="VIF08_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8134663.1"
                     /translation="MPFNIGPFELLLVLLLVLLVLGPGKLPEVGSALGRTIREFRKAS
                     TDVENSLNVDPSKAAAVSAPVRPAADNAAAAAEDKPAS"
     gene            complement(5004..5882)
                     /gene="pyrF"
                     /locus_tag="VIF08_01395"
     CDS             complement(5004..5882)
                     /gene="pyrF"
                     /locus_tag="VIF08_01395"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY8134664.1"
                     /translation="MSSQPSALAEPAGTADQATYLGRLGARSAAVGTTLCVGIDPDPE
                     ALPAGFSRDVSGIERFAGLILEAALSRAAAVKFNLAFYEAFGSGGIAALERLRARVPA
                     NVPVIADAKRADIGSTAARQAVAIFDALGADAVTLNPYLGRDALEPFLAHHDRFAYVL
                     CRTSNPGAAEFQGLDVDGQPLYLAVARRVAGWGGEGAPVGLVVGATAPAELAQVRAAA
                     PELPFLVPGVGAQGGDIDAVLADGPTAAGGGARARGGALLVNVSRGISAAAADGADPV
                     RGMVAAVDHWAGVLRC"
     gene            complement(5854..6492)
                     /gene="secF"
                     /locus_tag="VIF08_01400"
     CDS             complement(5854..6492)
                     /gene="secF"
                     /locus_tag="VIF08_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY8134665.1"
                     /translation="MADLRFALEDEFGPIDDVRQEASIGPIISGELTWQALILFVVGS
                     IGILSWMTFRFADFRMGVSALVALVHDIIVVVGIFAVLGTFFHLEIDALFVTAMLTVI
                     GFSVHDTIVVFDRVRENRIRHAGEPFSAIVNHSLLQTVGRSLNTSLTTIMVLTALLLF
                     GPHSITSFVFAMVIGIISGTYSSIFNASALLVTWHEWSEERRQREFATVRAR"
BASE COUNT         1078 a         2285 c         2355 g          934 t
ORIGIN      
        1 agcagcccgc gccagacggg catgtgtgcc tgggcgatgg cgtcgtcggc cggacgcttg
       61 ccgatgccaa agccgtcgat gatgaccagg acgagcggcc gcggtcgggt cacgcgcttt
      121 gccgggccgc ggcggtaatg cccgcgcgag cgacgatgcc ggccatctcg tcgactttga
      181 ggctcgcacc gcccaccagg gcgccgtcga tctcaggctc tgccaggaac tcgtcgatag
      241 ccgcgctggt gacgctgccg ccatagagga tgggcacgtg cgcggccgcc ccgggcatgc
      301 cggcgccggc aagcgtctcg cggatcagct gggccatcgc tgacgcgtcg ctgccgcgcg
      361 ccgtgagacc cgtgccaatg gcccacaccg gctcataggc gatcaccaga tcgctcggga
      421 tctcgcctga ggcccgggcg atctcggcca gggtgcgctc gagctggcgc cgcacggtct
      481 gctcggcctt gcccgccctg cgctgctcga tctgctcgcc gacgcacagg atgggccgca
      541 gcccaaactc cgcgcagcgc agcagcttac gaccgatcaa cacgtcgtcc tcagcctggt
      601 cgcggcggcg ctccgagtgg ccgatgattg cccacgacgc gaggcccgcg atcattggcg
      661 cggagatctc gccggtgtag gcgccgaagg gctccgcgtg gatgttctgg acaccgaccg
      721 aaacgctggt gtcctgcaac gcggagctga ctgcctgcag gcatacgaag ggcgggcaga
      781 tcacgcggat aacgccctcc acctccgtcg cggccgcgat gtcgttggcc aggagcgcgg
      841 catcaagtgg cgtcgtgttc atcttccaat tgccggcgac gatgacgggg cgcggttgtg
      901 tcctggcggc gcgcgtgctc attcggctcc ctcgtgcaat gcggcgctct cgatcagccc
      961 gaaggcgcgc tggacctggc cgcgcgacag atcgaggtcg gcggcgagct ggctgaaggt
     1021 ggcctcaggc gcgcggcgcc gggcgcgcgc gacaaggcga gcctggcgcg gcaggcggcg
     1081 cagcgtgccc tggcgctcga gcgcatcgat ctccgccagc tggcgccggg cggcgccgac
     1141 cgcgcgccgc agattggcgt gctcggcatt gacgacgcgg ttgagctggc ccgccaccga
     1201 gcggccgatc agccgcgtct ccagctcgag cgtcacagct gacgcgccca ggcggcgcag
     1261 caacgcgacg atcgtctcgg cgctcttcca ggtaaggacg ccgcggccgc ggcgcacgcg
     1321 cgcaccggcc gggtagccat gggcggcaag ccactcgccg agcggctcga ggtcttgggg
     1381 accagtcgtc agctcgaggt gcgtgccggt cggcgtgacg ctgagcgagc cgtgcgccag
     1441 gagcgcgccg cgcagccacg ccacgcggtc gtgctcgggc gcggacgccc aatcgaagct
     1501 cccctccccg gcagcgtcgt caaggcgcac ggccagccgt cccagcaggg gcgtccgcgc
     1561 ccgacccagc gccgcggcgc caagaccgtc ccgctccgcc gcgcggcagc agcgccgcgc
     1621 cgggtcgacg gctgcgagct cggcgcgcag cgcggtcgcc aggtccgggg tcgcggcgct
     1681 catcgggctt tggtcgtcgc gcgggcgcgg cgggcgatcg cgcgctcgtc gtacagcgtg
     1741 agcaacacgg atgcgagctt gcgcgaatcg tgacggtgcg cgttgtcgtc gtcgaccacg
     1801 tcccgggcga tgatctgcgg tcctgtcttc gatgacagcg gcacgtcgat gcgcaccggc
     1861 gctgcgggat agttgtgcgg ctgccgcgcc cggaagttgt cgttgaccag caccgcgtcg
     1921 atgacgtcgg ccatgccgtg gccacccagc gcgtccaagt gatcggagag cgcaaactcc
     1981 tcggtctcgc cgacctgggt ggcgacgttg cacaccaaga gccgcggcgc cgacgcccgc
     2041 gtcagtgcgt catggatgcc cggcacgagc agcggcggca gcaggctcgt gtacaaactg
     2101 cccggcccga cgatgatgag gtcggccgcg gcgatcgcct ccagcgcctc cgcggacggc
     2161 ttgacgtcct cgggcgagat ccagacgcgc cgaataccgc gcttccgggc aatctcggac
     2221 tggccttcca tgaccgaccc atcgatcagc gccgcgtgca gggtgatggg ctggccggcg
     2281 acgggcacca cgctgccgcg cacggcgagc acgcggttcg attggcggac gccctcttcg
     2341 aagtcgccgc tgaccgcggt caaggcggcg atgagcaggt tgccaaaggc atgcccggcg
     2401 taggaggaat cgaccgagtc ggccagcggg aagcgatacg acagcaggcg cgccatctgt
     2461 ggctccgtgt cggccagggc ggcgatgcag ttgcgaatat cgcccatggg cggcatgccc
     2521 agctccgttc gcagcttgcc cgaggagccg ccatcgtcgg ccacggtcac gaccgcggtg
     2581 atgttgctgg tcagctcctt caggccgcgc agcagcgcgc tcagcccggt cccgccgccg
     2641 atcgccacga cgcgcggccc gcgcgcccgc agccggcgct ggtagagcag ctcggccagc
     2701 ggctcctggg ccgagcgata cggctcgaca agggcgcgca gcagccgcca gctgctgtag
     2761 atgaacaaga tcaggccgag cccgagcggc agcatccagc gcagctccgg cggcagcgac
     2821 tggagcgtca ggatctcgat gatgtcgtgc agcggatcgt cggccgagcg gtcggccagg
     2881 gctgtgcgca gcagcgccgc gacagccagc gctacaagga agaggccaat gaagcccagg
     2941 acgagccagc gcttgacgcc cacgccgggg cgcagccact ggcgcaggac ctgcgcacgc
     3001 tcgcgccggc gcagtgccgc ctcgaggcgg cgcgcatcgt gggatgggtc ggcggacggg
     3061 cctgcgccgt tcacgagcgc tcgagttcgc gatgccagac gctcacgggg ccgcccttct
     3121 ccctgcgcaa gcgcgcggcc acttcctcgg cgatcgcgat cgagcggtgc tgaccgcccg
     3181 tgcagccaaa agccacggtc aggcggctgc gaccttccga ttcgtagccc ggcagtgtca
     3241 gctcaaggaa gcgcatgacc tcgtcgagga agcgcggcgt gagcggctgg tccaggacga
     3301 agtcgcgcac ctgtgggtcg agcccgttga gtggccgcag ccgatcgatg tagaacgggt
     3361 tctccatgaa tcgcacgtcg aatacgacgt cagcctcgag cgggatgccg tgcttgtagg
     3421 cgaatgagac gagctggagc gtgatgcctt cccgtccggt cggtgtgctg agcgtgctgg
     3481 tcagccgctc cctcaactgg cggccggaca tgtcggacgt gtcgacgatc acgtcggcct
     3541 ccgcgcggat ctcggccagc agctcgcgct cgcgcgcgat cgacgccgcc acgccgcgct
     3601 ccgcggtatc gagaggatgg cggtggcgcg tctccgagaa gcgccggatc aggacatcgt
     3661 cgcgcgcctc gaggaagaag acctgcggcg tgatgccgcg cgactcgagc gcgtccatcg
     3721 cctggtggaa cgcgtcggcc acgtcgccgg cgcgcgcatc cagcaccagc gcgactcgtt
     3781 cgtagcgctc cggctcacgc gccacgagct gggccaggtt gcgcagcagc tcggcgggca
     3841 ggttgtcgac cacgcgatag cccaggtcct cgagcagctt gctggcggcc gtcttgccgg
     3901 cgccggacag gccgctgatg atcgcaacgt gtgacttggt gtgcttagcc accggcgccc
     3961 tcctccgccc tcgatttgtc gccgcgcgcg acgagtcgga tggtcagctc gtacaggatg
     4021 tacatcacca agcccatgac ggtcggactg tacggatcgc cgcccggggt gaccacgacc
     4081 gcgaagacga atatggccag gaatacgtag cgccggttcc ggcgcagccg ctcagcgctc
     4141 acgataccga ccttgctcag cagcaccagc acgatcggaa actccatcac cagcccaaag
     4201 gccaggaaga ggttggtcac gaagccgaag taggcatcgg cagtgatcag cgggtccatg
     4261 acacccggga tcgcgaaacc gagcatgaag cccactgcga acggcaggat gaaccacgcc
     4321 acggagacgc ccagcgcgag gaacaccagg cccagcggta cccacggcag tgccagccgg
     4381 cgctcgcgcg gcgtcagacc gggcgcgatg aacgcccaga gttggtagag caggatgggc
     4441 gaggccaagg cgatgccgat catcaacgcg agcttgagct ggatgaagaa cgcgctgctg
     4501 ggcgccgtga agtacagcgg ctggctgatg ggtgtcttca ggaagcggat cgcgtccggc
     4561 gcgagccaga agccgaccgc cgcgccgatt gcgatcaccg cgaagctgac gatgatgcgt
     4621 cggcgcagct ccgagagatg ctcgacgacg gtcatgattt tgccgtcgcc ggaatccggc
     4681 gggtgatcgg tcactaccgt ctcggtcatg gcgcggatgg ttctccgcgg cctatcgggc
     4741 gcgggtcagg aagccggctt gtcctcggcc gcggctgcgg cgttgtctgc cgcaggccgg
     4801 accggagcgc tgacagcggc cgcctttgac ggatcgacgt tcagtgagtt ctcgacatcc
     4861 gtcgacgcct tgcggaactc gcgaatggtg cggccgagcg cgcttccgac ttcgggcagc
     4921 ttgcccgggc ccaggacgag caggaccagg agcagtacca gcagaagttc gaaaggaccg
     4981 atgttgaacg gcacgtcaga atcctagcac cgcagcaccc cggcccagtg atcaaccgcg
     5041 gcgaccatgc ccctgacggg atccgcgcca tctgcggcgg ccgctgagat cccgcgcgat
     5101 acgttgacga gcagcgcacc gccgcgcgct ctggcgccac ccccggctgc ggtcgggccg
     5161 tccgccagca ccgcatcgat gtcgccgccc tgcgcaccca ccccgggcac gaggaacggc
     5221 agctctggcg ctgcggcacg aacctgggcg agttctgccg gcgcggtcgc accgaccacc
     5281 agccctacgg gcgctccctc cccgccccag cccgcgacgc gccgcgcgac cgccaggtac
     5341 agcggctggc cgtcgacgtc gagcccctgg aactccgccg cgcccgggtt ggacgtccgg
     5401 cacagcacgt acgcgaatcg gtcgtggtgg gcgaggaagg gttcgagcgc gtcgcgccca
     5461 agatatggat tgagcgtcac cgcgtcggcg cccagcgcat cgaagatcgc cacggcctgg
     5521 cgcgcggcgg tgctaccgat gtcggcgcgc ttcgcatcag cgatgaccgg cacgttcgcg
     5581 ggcacgcgcg cgcgcagccg ctcgagggcc gcgatgccgc cggatccgaa cgcctcatag
     5641 aaggcgaggt tgaatttgac tgcggctgcg cgcgacaagg ccgcttccag gatcagcccg
     5701 gcgaagcgct cgatgccgga gacgtcgcgc gagaaaccgg ccggcaacgc ctccggatct
     5761 ggatctatgc ccacgcacag cgtggtgccg acagcagcgc tgcgggcgcc cagccggccc
     5821 aggtatgtcg cctggtcggc tgtgcccgcg ggctcagcga gcgcggacgg ttgcgaactc
     5881 acgctggcga cgctcttcgc tccactcgtg ccacgttacc agcagggcgc tcgcgttgaa
     5941 gatcgatgag tacgtgccag agatgatgcc gatgaccatt gcgaacacga acgacgtgat
     6001 cgagtgcggg ccgaacagca gcagtgccgt cagcaccatg atcgtcgtca acgacgtgtt
     6061 gagcgaccgg ccgacggtct ggagcaggct gtgattgacg atcgcgctga atggctcgcc
     6121 agcgtgtcgg atgcggttct cacgcacgcg gtcgaagacc acgatcgtgt cgtggacgga
     6181 gaagccgatg accgtcagca tcgccgtcac gaacagggcg tcgatctcga ggtggaagaa
     6241 cgtgcccagc acggcgaaga tgcccacgac gacgatgatg tcgtgcacca gggcgacgag
     6301 cgccgaaacg cccatgcgaa agtcggcgaa gcggaaggtc atccagctga ggatgccgat
     6361 tgagccgacc acgaacagga tcaacgcctg ccacgtcagc tcgccgctga tgatcgggcc
     6421 gatggacgcc tcttgccgca cgtcgtcgat cggcccgaac tcatcctcca acgcgaagcg
     6481 caggtcggcc aagcggccct cagtcggcag gccgatcggg ttcgccgtcg gcgatggtga
     6541 agggctgacc gcgggcgacg cgctgggact cgctgccgcg gatgggctgg gactggctgc
     6601 cgcagatggg ctcgcgctgg gcgtcgcact cgtcgtggca cttggcgcgg gc
//
LOCUS       DATBRX010000041         8040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_907308, whole genome shotgun sequence.
ACCESSION   DATBRX010000041 DATBRX010000000
VERSION     DATBRX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907308"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VIF08_01405"
     CDS             <1..766
                     /locus_tag="VIF08_01405"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEY8134666.1"
                     /translation="SLLTRDTGHPGSLGVAISEAIEDTVTHPGTKYSLGSVFDFVLLH
                     QTVIGQEAILQMEMAGEEPDTVIACVGGGSNFAGLAFPFIGRQLRGEGRYRVIAVEPE
                     AAPSLTKGRYEYDFGDTGEMAPLVKMHTLGHHYVPPPIHAGGLRYHGMAPLVSKLVDD
                     GLVEARAVHQNATFEAGLRFARAEGIIPAPESNHAVRAAIDEALEAKAAGEKKVILFN
                     LSGHGHFDLAAYEAYLTGRLEDFEYRPTEAREAVAA"
     gene            842..1423
                     /locus_tag="VIF08_01410"
     CDS             842..1423
                     /locus_tag="VIF08_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134667.1"
                     /translation="MATPDELVLVLARDRVPGGLDFTGVADRPFAPVLEAVRQFGAFR
                     PRGKVEDDPSLKQIIPYLVLHDGERTFLMKRTSAGGDERLHERYSIGVGGHVNPGDAD
                     VLGGLNREWREEIAADFVPEFEPLGILNDDSNPVGAVHLGLVYAADAAGRPVAIRETH
                     KLSGEFVSMDAVAGVRDRLETWSALLFDFLSVS"
     gene            1438..2802
                     /locus_tag="VIF08_01415"
     CDS             1438..2802
                     /locus_tag="VIF08_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY8134668.1"
                     /translation="MNTVRFRSRLRWLGRAFFAAGATLLLAGASLADEARPQVYVMTT
                     TGIVDHVMADYLHDGFERAANEGFAAVIIELDTPGGELGATDAIVQSILDAPLPVITW
                     VAPVGARAASAGTFITIAGHVAVMAPGTRIGAAAAISGDGTDLPDTLADKVREDSLAM
                     LRAIKELRPSRNTDWAYTTVIDAKSYAGAEALAAGGIDGICGTLDEVLAFANGRSVIV
                     KGQPMTLALADAVTTDLPMNVQQNLLRFLADPNIAFILFTVGVYGLLFELQNPNFVTG
                     TVGAIAIVLAVIGFGSLPVNIGGLVLVGIGIVLFLLEPTVTSHGLLAIGGIVCFALGA
                     AALYTDPVLPGAPDISVALPVILTMTVLTGLFMVVVVWTAIRTRHMTTAQGLIGAGLP
                     ANTMGEVRRPLTPLGSVYAGGEEWSAKTSDERPLDRGTPVRILRQEGLTLIVEPGKEQ
                     TTNG"
     gene            2795..3688
                     /locus_tag="VIF08_01420"
     CDS             2795..3688
                     /locus_tag="VIF08_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8134669.1"
                     /translation="MVDTVTLAIVIAVLVFLLLALYLSIRVVQQYERMVIFRLGQTGP
                     NLVRDPGLRFLIPVIDRPYKVDIREKFIEIPSQTTITRDNAPINIDFLIYWYIVDPLK
                     SIVNVQNFAGALQGIATTTLRAVIGDILLDDVLSKRDQINEVLRAKLDEQTERWGGKI
                     TTVEIREIIPPRDVQEAMNRMLSAERTRRAVITESEGTRQANINVAEGEKQANILRAE
                     GKRQSQILEAEGYSGALQQIFGAARLVDEKTMTLQYLEALKALGASPSTKYVIPVELV
                     ELANRITGFTTAGLEAGKQVK"
     gene            3977..5170
                     /locus_tag="VIF08_01425"
     CDS             3977..5170
                     /locus_tag="VIF08_01425"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8134670.1"
                     /translation="MPARIQLVATQPAIQQQLGSMLRHDGHQVIIAPDGPAGLRQWTA
                     ERPDLVLCDNDLVGPSGMELVMRIRQAEGSGAHTPIILLGGGTDVDAKVAALRSGADD
                     YLAKPIHPQELSARVRGLLTRFQRAQPQPQPNRPTSGRVVAFYGAKGGVGTTTLAINA
                     AIAAQRELKRSVCLVDANLQFGDHRVFLDLGPDSRSIVDAVSAGTVELDMLRQSIVRH
                     ETGVELLLAPSSPEHAELVSQSQHHLLKVVEMLRTVYELVIVDMDERFDDHLLDVIGI
                     ADRLVVVLTADLSCVKNVRLVLETMAQLRVPRERLMLVLNRANAFTGISVKSVENVLR
                     RPIEHQVVNDYRSAISALNSGTPFMAKRPDSALGKDVLELARLIDKPAAATEELKALE
                     LSPVR"
     gene            5251..6852
                     /locus_tag="VIF08_01430"
     CDS             5251..6852
                     /locus_tag="VIF08_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY8134671.1"
                     /translation="MLATLMTAVINGLRGELVRVEIDVAPGLPVCHIVGLPDAALSEA
                     RERVRGAIRNSGFEYPMSRITVNLAPADRRKRGAAYDLAIAIGILVASTQIRRGGGTW
                     ALLGELSLSGTAEPVAGVLPMVATLARQGYRRVIVPAANELEARLVSGVEVHGVEGLD
                     DAARLVAGPRGRTAARAVRRSAVELSGPRGVGAAGAAARAGTTLTARLAPVDLAEVRG
                     QHQARWALEIALAGGHNLLLVGPPGAGKTLLARAIPGLLPPLDDEEALEVTVIESVAG
                     MLDGGSLEQGLRRERPFRAPHHTSSYAALVGGGPALQPGEATLAHHGVLFLDELAEFD
                     RPTLDALRQPLEEGVLTIARARGHVRYPARFQLVAAMNPCRCGYFNDLQRRCVCKTGD
                     PESYVRRVSGPLRDRFDMEIEMSRVPPKVLLSGPSPEGSAAVRARILAARHVALARNG
                     GRPNALLPGTAVLAAAAMTRSATEVLEEFAATYHLSARSVHRLLRVARTMADLAGRSS
                     VSEHDVRAAGSLRDPAAPAAQPLAA"
     gene            6849..8012
                     /gene="dprA"
                     /locus_tag="VIF08_01435"
     CDS             6849..8012
                     /gene="dprA"
                     /locus_tag="VIF08_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY8134672.1"
                     /translation="MNTRDEWICLASVQGVGEETFNSLIGEFGSAGAALEAVRDGRFD
                     SWFQRRRQEDGRVPMIKSAVAALRATAANPTQPLQAISALGLWTYTSLDTDYPARLRD
                     LPDPPPVIHGLGDRQALHGLRTVGIVGTRRPTPQGRALAAKIAARVVEFEATVVSGLA
                     VGIDGAAHAATLDNEGRAVGVIGGGHHFPGPRAHQRLRDEVVARGGAVISEHHPDDHP
                     KLGTFPRRNRIIAALSDAVLVVEAPTRSGAINTANHALGLGRPVFVAPGRVGEWSVAG
                     ALKLLHDTPARPLIGLDELVEDLGYLSVPVAAEDSEEQQAVSSREAALATLGAAERAI
                     ARRLAEGPAGLDLLVAETGYGPQVVSSAVTFLLMRGWVHAAGPAYIAAGPLAA"
BASE COUNT         1272 a         2723 c         2768 g         1277 t
ORIGIN      
        1 gtcactgctg acgcgcgaca ccggccaccc gggttcgctc ggcgtggcca tcagcgaggc
       61 catcgaggac acggtcaccc atccggggac caagtactcg cttggctcag tgttcgactt
      121 cgtgctgctg caccagacgg tcatcggcca ggaggcgatc ctgcagatgg agatggccgg
      181 cgaagagccg gataccgtca tcgcctgcgt cggtggtggc tcaaacttcg cgggcctggc
      241 cttcccgttc atcgggcgcc agctgcgcgg ggaggggcgc taccgcgtca tcgccgtcga
      301 gcccgaggct gcgccgtcgc tgacgaaggg ccgctacgag tacgacttcg gcgacaccgg
      361 cgagatggca ccactggtca agatgcacac cctgggccac cactacgtgc cgccgccgat
      421 ccacgccggc gggctgcgct accacggcat ggcgccgctg gtcagcaagc tcgtcgacga
      481 tggcctggtg gaggcccgtg ccgtgcacca gaacgcgacc tttgaagctg gtctgcgttt
      541 cgcccgagcc gagggcatca tccctgcgcc agagtcgaac cacgcggtcc gcgcagcgat
      601 cgacgaggcg ctcgaggcca aggccgcggg cgagaagaag gtaatcctct tcaacctgtc
      661 aggccacggc cactttgacc tggcggcata cgaggcgtat ctgaccggtc gactggaaga
      721 ctttgagtac cggcctaccg aggccaggga ggccgtcgcg gcctagctgc tcccacctct
      781 gggcggaagc cttgtcccct ccgggcctcc gcccatcttc gtcggtcatt agagtcagcg
      841 cgtggcgacg cccgacgagc tggtcctggt cctggcgcgc gaccgggtgc cgggcgggct
      901 cgatttcacg ggcgtggccg atcgaccgtt cgcgcccgtc ctcgaggcgg tgcgccagtt
      961 tggtgcgttc aggccgcgcg gcaaggtcga ggacgatccc agcctcaagc agatcatccc
     1021 ctacctggtg ttgcacgacg gcgagcgcac tttcctgatg aagcggacca gcgccggtgg
     1081 cgacgagcgg ctgcacgagc gctactcgat cggcgtgggc ggccacgtca acccgggtga
     1141 cgcggacgtg ctgggcgggc tcaaccgcga gtggcgcgag gagatcgccg cggacttcgt
     1201 gccggaattc gagccgctgg gaatcctgaa cgacgactcg aacccggtcg gcgccgtcca
     1261 ccttggcctg gtctacgccg ccgatgctgc cggtcggccg gtcgcgattc gcgaaacgca
     1321 caagctgagt ggcgagttcg tgtccatgga cgccgtcgcc ggcgtgcggg acaggctcga
     1381 gacctggagc gcgctcctgt tcgatttcct gtctgtctca taatcaacaa cagacagatg
     1441 aacaccgtcc ggttccggtc acgcctgcgc tggctgggcc gcgctttctt cgcggcggga
     1501 gccacccttc tccttgccgg cgccagcctc gctgacgagg cccggccgca ggtctacgtg
     1561 atgactacga ccgggatcgt cgaccacgtc atggccgact acctgcacga cggcttcgag
     1621 cgggccgcga acgagggctt cgccgccgtc atcattgagc tcgatacgcc gggcggtgag
     1681 ctgggcgcca ccgacgccat cgtgcagtcg atcctcgacg cgccgctgcc ggtcatcacc
     1741 tgggtagcgc ccgtcggggc gcgcgcagcc agcgccggca ccttcataac gattgccggg
     1801 cacgtggcgg tcatggcgcc cgggacgcgc atcggtgcgg cggccgcgat ctcaggcgac
     1861 ggcacggacc tgccggacac ccttgccgac aaggtccgcg aagacagcct cgcgatgctg
     1921 cgcgccatca aggagctgcg cccgtcgcgc aacaccgact gggcctacac gacggtcatc
     1981 gacgccaagt cctatgccgg cgccgaggca ctggcggcgg gcggcattga tggcatctgt
     2041 gggacgctcg atgaggtcct cgcatttgcc aacggccgca gcgtgatcgt gaagggtcag
     2101 cccatgaccc tcgccttggc cgacgcggtc acgaccgacc tgccgatgaa cgtgcagcag
     2161 aacttgctgc gcttcctggc cgacccgaac atcgctttca tcctcttcac cgtgggcgtc
     2221 tacggcctgc tcttcgagct gcagaacccc aacttcgtca ccggcacagt gggcgcgatt
     2281 gccatcgtcc tggcggtgat cggttttggc agcctgccgg tgaatatcgg tgggctggtc
     2341 ctcgtcggta ttgggatcgt cctgttcctg ctcgagccaa ccgtgaccag tcacgggctg
     2401 ctcgcgatcg gcggcatcgt gtgcttcgcg ttgggcgccg ccgcgctcta caccgaccct
     2461 gtcttacccg gcgcgcctga catcagcgtc gcgctgcctg tcatcctgac gatgaccgtg
     2521 ctgacggggc tgttcatggt cgtggtcgtg tggacagcga tcaggacgcg ccacatgaca
     2581 accgcgcagg gcctgatcgg cgccgggctg ccggccaaca cgatgggcga agtgcgccgg
     2641 ccgctgacgc cgctcggctc ggtctatgct gggggagagg agtggtcggc gaaaacgtcc
     2701 gatgaacggc cgctcgaccg tgggacaccc gtgcgcatcc tgcgtcagga ggggctgacg
     2761 ctgatcgtgg aaccaggaaa ggaacaaacc accaatggtt gacaccgtga cgctggcgat
     2821 cgtcatcgcc gttctcgtgt tcctgctgct ggcgctgtac ctgtcgatca gagtcgtgca
     2881 gcagtacgag cgcatggtta tcttccgcct gggccagact gggcccaacc tcgtgcgcga
     2941 ccccggactg cggttcctga tcccggtgat cgacaggccc tacaaggtcg acattcgcga
     3001 gaagttcatc gagatccctt cgcagacgac catcacgcgc gacaacgcgc cgatcaacat
     3061 cgacttcttg atctactggt acatcgtcga tccgttgaag tcgatcgtga acgtccagaa
     3121 cttcgccggc gccctccagg gcatcgcaac gacgacgctg cgcgcggtca tcggcgacat
     3181 cctgctcgac gatgtcctgt caaagcgcga ccagatcaac gaggtgctgc gcgccaagct
     3241 cgacgagcag accgagcgct ggggcggcaa gatcacgacg gttgagatcc gcgagatcat
     3301 cccgccgcgc gacgtgcagg aggcgatgaa tcgcatgctc tccgctgagc gaacgcggcg
     3361 cgccgtcatc accgaatctg aaggcactcg ccaggcgaac atcaacgtcg ccgaaggcga
     3421 gaagcaggcc aacatcctgc gcgccgaggg caagcgccag tcgcagatcc tcgaggcaga
     3481 gggctactcg ggcgccctgc agcagatctt tggcgccgcc aggctggtcg acgagaagac
     3541 gatgacactc cagtacctgg aggcgctcaa ggcgctgggc gccagcccgt cgacgaagta
     3601 cgtcatcccg gtcgagctgg tcgagctggc caaccgcatc accggcttca ctaccgcggg
     3661 cctggaggcc ggcaagcagg tcaagtagcc cgcgtcatcc gccgggcttg agccaaatag
     3721 acggaatacg accaccgctc acagccgaga gggcaagaag cctggatttg gctcacattt
     3781 ggtccttctg gcgcgaattg agcctgaata ttcgaatcgg tggcccagat gggtccattt
     3841 ggcccaaacc ggtcagtcgg aatcgccggc aggtgggcgg taatggtcct tccggacggg
     3901 tgcggggggt acgactgcag cgtgggtggg gcggtgagtc gggcctagga tcgctctcga
     3961 cgcacaacgg agagccatgc ccgcacgaat ccagctcgtc gccacgcagc cagccatcca
     4021 gcaacagctg ggcagcatgc tgcgccacga cgggcaccag gtcatcatcg cgcccgacgg
     4081 gccggccggt ctgcgccagt ggaccgccga acgacccgac ctggtgttgt gcgacaacga
     4141 cctcgtcgga ccgtctggca tggagctcgt catgcgcatt cgccaggccg agggcagcgg
     4201 cgcgcacacg cccatcatcc tgctcggtgg cggcaccgac gtggatgcga aggtggccgc
     4261 gctgcgctcc ggcgcggacg actacctggc gaagcccatc catccgcagg agctgtcagc
     4321 ccgcgtgcgc ggcctgctca cgcgcttcca gcgtgcccaa ccgcagccgc agccgaaccg
     4381 cccgacgagc ggccgcgtcg tcgcgttcta cggcgccaag ggcggcgtcg gcacgaccac
     4441 gctcgccatc aacgccgcca ttgccgcgca gcgtgagctg aagcgcagcg tgtgcctggt
     4501 cgatgccaac ctgcagttcg gcgaccaccg cgtgttcctg gacctggggc cggactcgcg
     4561 ctccatcgtc gacgcggtga gtgccggcac ggtcgagctg gacatgctgc gccagtcgat
     4621 cgtgcgccac gagaccggcg tcgagctgct cctggcgccc tcgagccccg agcacgccga
     4681 gctggtcagc cagtcccagc accatctgct caaggtggtc gagatgctgc gcaccgtcta
     4741 tgagctggtc atcgtcgaca tggacgagcg cttcgacgac cacctgctcg acgtgatcgg
     4801 tattgccgac cggctcgtcg tcgtcctgac ggctgacctg tcgtgcgtca agaacgtgcg
     4861 cctcgtgctc gagacgatgg cccagctgcg cgtgccgcgg gaacgcctga tgctggtcct
     4921 taaccgcgcc aacgcgttta cagggatcag cgtgaagagc gtcgagaacg tgctgcggcg
     4981 gccgatcgag caccaggtcg tcaacgacta ccgttcggcg atttccgcgc tgaacagcgg
     5041 cacgcccttc atggccaagc ggcccgacag tgcgctgggc aaggacgtcc tcgagctcgc
     5101 gcggctgatc gacaagccgg cagctgccac cgaggaactg aaggcgctgg agctgtcccc
     5161 ggtccgatag gacgagcgct cctcttgcga taagtcgggg gtaagcaccg actaaccgtc
     5221 aggcaagcgg gcaacggtac gttgccttgc gtgcttgcca ccctgatgac cgccgtgatc
     5281 aacggcctgc gcggcgagct ggtgcgcgtc gagattgatg tcgcacccgg gctgcccgtc
     5341 tgtcacatcg tggggctgcc ggacgccgcg ctgtcggagg cgcgcgaacg ggttcgaggc
     5401 gcgatccgca attccggttt cgagtacccg atgagccgca tcacggtcaa cctggcgccg
     5461 gctgataggc gcaagcgcgg cgcggcttac gacctggcga ttgcgatcgg gatcctggtc
     5521 gcatcaacac agatacggcg cggcggcggc acgtgggcgt tgttgggcga gctttcgttg
     5581 tccggcaccg cagagccagt ggcgggcgtg ctgcccatgg tcgcgaccct ggcgcggcag
     5641 ggctaccggc gcgtgatcgt gcctgccgcg aacgagctcg aggcgcggct ggtttctggt
     5701 gttgaggtcc acggcgtcga gggactcgac gatgccgcgc ggctggtggc cggaccgcgc
     5761 ggtcgtacgg cggcgcgggc ggtgcgccga tcggctgttg agctgagcgg accgcgcggc
     5821 gtgggcgcgg cgggtgccgc ggcgcgtgcc gggaccacac tgacggcacg ccttgcgccg
     5881 gtggacctgg cggaggtgcg cggccagcac caggcgcgct gggcactcga gatcgcgctc
     5941 gcgggcggcc acaacctgtt gctcgtcggc ccgccgggcg ccggcaagac gctcctcgca
     6001 cgggcgatcc ccggattgct gccgccgctc gacgacgagg aggcgctcga ggtgactgtc
     6061 atcgagagcg tcgccgggat gctcgacggc ggctcgctgg aacaggggct gcgccgtgag
     6121 cggcccttcc gcgcgccgca ccacacgtct tcgtacgcgg cgctcgtggg cggcggacct
     6181 gcgctccagc ccggtgaggc gacgctcgcc catcacggcg tcctgttcct cgatgagctc
     6241 gccgagttcg accggcccac gctggacgcc ctgcgccagc cgctcgagga gggcgtgctg
     6301 acgatcgccc gggcacgcgg ccatgtccgc taccccgcgc gcttccagct ggtcgcggcg
     6361 atgaacccat gccgctgcgg ctacttcaac gatctgcagc ggcgctgcgt ctgcaagaca
     6421 ggcgatcccg agagctacgt gcgccgggtg agcggcccgc tgcgcgaccg gttcgacatg
     6481 gagatcgaga tgtcgcgcgt gccacccaag gtcctgctga gcggaccaag cccggaggga
     6541 agcgccgcag tacgggcacg catcctcgcg gcgcgccacg ttgccctggc gcgcaacggc
     6601 gggcggccca atgctctgct ccctggcacc gctgttctgg ccgcggccgc catgacgcgc
     6661 tctgcgacgg aagtgctcga ggaattcgcg gccacttatc acttgagcgc gcgatcggtg
     6721 caccgtctcc tgcgcgtcgc gcggacgatg gctgacctgg ccgggcggtc cagcgtgagc
     6781 gagcacgatg tcagggcagc aggcagcctg cgcgacccgg ccgcgcccgc cgcccaacct
     6841 ctcgcggcgt gaacacacgc gacgagtgga tctgtctcgc atcggtccag ggcgtaggcg
     6901 aggagacgtt caactcgctg atcggtgagt tcggcagcgc gggcgcggcg ctggaggcgg
     6961 tgcgtgacgg tcgctttgac tcgtggttcc agcggcggcg ccaagaggac ggtcgcgtgc
     7021 cgatgatcaa gtcggctgtc gcggccctgc gcgcgaccgc cgcgaatccg acccagccgt
     7081 tacaggcaat cagcgcgctc gggctgtgga cgtatacatc gctggatacc gactacccgg
     7141 cacggttgcg cgacctgccc gacccgccgc cggtcatcca cggcctgggc gatcggcagg
     7201 cactgcacgg attgcggacg gtgggcatcg tcggcactcg gcgacccacg ccgcagggtc
     7261 gggcactcgc agccaagatc gcggcgcgcg tcgttgaatt cgaggcgacc gtggtgtcgg
     7321 gactcgcggt gggcatcgac ggcgcggccc acgccgccac cctcgacaac gaaggcaggg
     7381 cggtcggcgt gatcggcggt ggccatcact ttccggggcc gcgcgcccac cagcggttgc
     7441 gcgacgaggt cgtcgcgcgc ggcggcgccg tcatcagcga gcaccacccg gacgatcatc
     7501 caaagctcgg cacgttcccc aggcgtaacc ggatcattgc cgcgctgtcg gacgccgttc
     7561 tcgtggtcga ggcaccaaca cggagcggtg cgatcaacac ggccaaccac gcgctgggcc
     7621 ttggccggcc ggtattcgtc gcgcctggtc gcgtcggtga gtggtcggtc gcgggcgcgc
     7681 tcaagctgtt gcacgacacg cccgcgcggc cgctcatcgg gctcgacgag ctcgtcgagg
     7741 atctcggcta cttgtccgtg ccagtggcgg cggaggacag cgaggagcaa caggcagtca
     7801 gctcgaggga ggctgccttg gccacgctgg gcgcggctga gcgcgccatc gcgcggcgtc
     7861 tggctgaagg gccggctggc cttgacctgt tggtcgcgga aaccggctat ggcccgcagg
     7921 tggtgtcgag cgcggtgaca tttctactga tgcgaggttg ggtgcacgcg gcgggtccgg
     7981 cctacattgc cgcaggcccg ctcgccgcat gacgccccct gacctacgta ccgttgcccg
//
LOCUS       DATBRX010000042         8842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3365884, whole genome shotgun sequence.
ACCESSION   DATBRX010000042 DATBRX010000000
VERSION     DATBRX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3365884"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..723)
                     /locus_tag="VIF08_01440"
     CDS             complement(16..723)
                     /locus_tag="VIF08_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134673.1"
                     /translation="MPDGTVPQRIRPRHPRLRLLGLVAVAYGLVGLLLFVTVAVAING
                     PLERAHALSASLDEERAGLVATMEQARATLVDMSLGVGGVDESLASAQAATDRAAGIS
                     TSLASSMFGLRDSMSISILGTQPLQSLAAGFDASGQQLSALATDLATISSSLSTNRGA
                     VETTSESLGKLAVSLAELRVLVRDSPDVEISAASLDAIRLAVYAICGWLAVLALGCVV
                     GGLYLYRLGGRGRLPAE"
     gene            complement(716..1918)
                     /locus_tag="VIF08_01445"
     CDS             complement(716..1918)
                     /locus_tag="VIF08_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8134674.1"
                     /translation="MTDLLLGPFKSIGRVWAQVGKELIQVRRRPGAFFSLVLGPFLIM
                     AVFGLGYTGVRRPLETVVVIPSNLSLPREVAFYQDLAGPALHIVRVDETPDAAHAQLA
                     ARQLDLIVSAPADASEQFRAGRQAQIDVEFNSVDPVEAGNASFLAGLLDQQINREIIR
                     QAVSEGQSYLLEDVSPPVRVPPDVIAAPTTVHLTNIAPTQPNVVGFAAPAALALMLQH
                     MAVTLTALSFVRERMSGALELLRVSPLNSFELVLGKYLGLGAVSALIAAISVALLVGG
                     LGVPMLGSWLMVGLVIALIILASLGIGLIISVVSDSERQAVQLCLLLLLASVFFSGFV
                     LPVHEFKEGVQYGSYVLPVTHGIRLLQDFMLRGDTTAWWQLGALAALAVAFLILTIVL
                     LRRTLRSA"
     gene            complement(1915..2925)
                     /locus_tag="VIF08_01450"
     CDS             complement(1915..2925)
                     /locus_tag="VIF08_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8134675.1"
                     /translation="MPRASRMPAPPGPPPVVLDHVSRSFGKVLAVDDLSLMAAPGSLL
                     GVIGPSGSGKTTTIRLMLGALKPDRGVIRVLGEDPRHFHRRTRESIGYMPQSFVLYPE
                     LTARENVNHMAALFGIGPLRRGRRVAEVLELVDLGEARDRRASDLSGGMQRRLELACA
                     LVHKPQLLVLDEPTAGIDPLLRTRIWHELDHQRREGATVLVTTQYVTEAEYCDAVALI
                     SGGRLIAHATPTDLRRMALGGEVIEVGTSAPFDARSLPPIEGVVRVRQMGPAQMLVIA
                     HDAGVASPRVAAAVAAAGGHVDYSREYRPTFDEVFAALVTSDAEARAEAESDAEAQAS
                     FE"
     gene            3229..4275
                     /locus_tag="VIF08_01455"
     CDS             3229..4275
                     /locus_tag="VIF08_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134676.1"
                     /translation="MADEGRDAATASDGVEAARAAGLRYADPAEPGIRRIRRGRGFSY
                     RDAAGRLVKDPATLARIRALAIPPAWREVWICASARGHIQAMGRDARGRRQYRYHADW
                     TKARDQTKFERSIAFARALPRLRRRVARDLRRPGMPRDKVLAAVVRLLDRTLLRVGNE
                     RYARENSSFGLTTLRNRHARPDGRGGMRLSFRGKGGKRFEVGLSDRRLVRIIRACQDL
                     PGQRLFEYRDENGDERPVESEDVNDYIRAVTGDDFTAKDFRTWAATVLAAQELRRNDD
                     LPAVVRAVAEQLGNTPAVCRRSYINPVVLVPADPRPNAHVAPVLTGPSGLNSDERHVL
                     ALLRRARSAAARAA"
     gene            4307..7099
                     /locus_tag="VIF08_01460"
     CDS             4307..7099
                     /locus_tag="VIF08_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8134677.1"
                     /translation="MYRGQPARSRLRNGSNGNGNGHDRGNGHGRRNGRGNPNVRRIAI
                     ARVRSRSAPPNRLGLYAVLALAGVVGFFGAIGGSIGLGTTTGLSFIAQMEAELPPVSN
                     FEQLDFAQPTVVYDRTGTVELARFQIERRRVVAYEAIPKVLLDATIAVEDQTFWDNEG
                     YDPNAILSAALESLAGEGDRGASTITQQLVRQRLLDPELVADPDRLLERKVKEILLAR
                     NLTSAYPGDEGKQRILTAYLNQIYYGHAAYGIAAAAEVYFGVSDLKLLTPAQAAILAG
                     LPQAPNSYDLYKWAETDANGQLVVPLVPTEAHPEIATPVKRRNKILENLERGDGHFIR
                     LTPQQLDQALAEPIVLKPPTPFELKAPHFVFYMKRQLEALVADRAPVERGGYRVITTL
                     DMGAQALAEKYVAAGTIVPNLPTGQMEAEIDAQGLGEDRRWIEALHGTDIHNGSLVAM
                     DARTGQVIAYVGSAGYYRDDIASPQFDPKYDVAGSAFRQPGSAWKPIMYAAGFDQRVV
                     TPGTLLNDVITEFSRDWYPRDADRKERGPVLMRDALTYSLNIPAIRALDRIGVDPVAS
                     LASAMDLDFLRERQLFQAGLASAIGTVEVKQVELTAAYGALANQGVYEPIQTILSITD
                     ANGNPVPVTPAAPTQVLTEQAAWLMSDILKDSTDPALNTVFGPRLQIINGIRDPLVPG
                     SERRPAAAKTGTANDLRDLLAFGYLGPPADPNAMHIVASVWTGNSDHSAPNAGDFSII
                     AADGPGRIWSSFLRELSRDWPMATFPAPPPGVVQATIDAWSGGAPGPWTRDTRTEWFI
                     EGTQPGGQFQVDPPGLLYTQMCGRWFVDIAKAEAADPPRWIEADLGWMERARRGTGVR
                     GENGGTTAHLFGRSDWGGFIAPIDCASAPQPTPQPPPDHTPGPGPTPPPGPTPPPGTP
                     KPTDPPPTEPPPTAAP"
     gene            7373..8113
                     /locus_tag="VIF08_01465"
     CDS             7373..8113
                     /locus_tag="VIF08_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8134678.1"
                     /translation="MINRRVGRIHRALRQRAHLTQRELSANSGVVRWKIGRIERDLVD
                     RLRFDEVERCLAALGAELEVRVSYRGAEVDRLIDRVHSLVGARVIEVLRRQGWEVQTE
                     VTFSEWGERGSYDILAWHPQSRTLLVIEIKSELASVEGTLRQLDVKVRLARSVARKRF
                     GWEARAIARVMVLPEDRSARRQVAANAALLRAVLPATSRQVRTWLRFPDRSLSGIWFL
                     SAADGRPWPRNPSSIRRVRSKAAAGATR"
     gene            8232..8618
                     /locus_tag="VIF08_01470"
     CDS             8232..8618
                     /locus_tag="VIF08_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134679.1"
                     /translation="MAQTGTRSTTTRKSTSTDTDNNENGTGQTQINAVVSGAQQAAEV
                     ARQTAERAAARLPDAVARTQVAARDTQVALEGMDGQTLLAGTTFSLGLGVGLFVSGAH
                     RLLVTAAFLPALAMLLTLLGRQREEA"
BASE COUNT         1428 a         3098 c         3095 g         1221 t
ORIGIN      
        1 tggtccgcgg ctgattcact ccgcgggtag tcggcccctt ccgcccagtc ggtagaggta
       61 gagcccgccg acaacgcacc ccagggcgag cacggccagc cagccgcaga tggcgtagac
      121 cgcgaggcgg atcgcgtcga ggctggccgc cgagatctcg acgtcggggc tgtcgcgcac
      181 caggacgcgc agctcggcca gcgacactgc gagcttgccc aggctctcgc tggtcgtctc
      241 gaccgcgccg cggttggtgc tgagcgatga gctgatggtc gcgaggtccg tcgccagggc
      301 ggacagctgc tgaccgctcg cgtcgaagcc ggccgcgaga ctctgcagcg gctgcgtgcc
      361 gaggatggag atcgacatgg agtcgcgcag gccgaacatg ctcgacgcga ggctggtgct
      421 gatgcccgcg gcgcgatctg tcgccgcttg cgcgcttgcc agcgattcat cgacaccgcc
      481 cacgcccagg ctcatgtcaa cgagcgtcgc gcgcgcctgc tccatcgtcg cgaccaatcc
      541 cgcccgctcc tcgtcgagcg acgcggaaag cgcatgtgct cgctcgagcg ggccgttgat
      601 cgcgaccgcc acggtcacaa agagcagcag tccgaccagc ccgtaggcaa ccgcgaccag
      661 tcccagcagg cgcagccgcg gatggcgcgg gcgaattcgt tgtgggactg tgccatcagg
      721 cactgcgcag ggtccttcgc agcagcacga tggtcaggat aaggaatgcg accgcgagcg
      781 cggcgagcgc gcccagctgc caccacgcgg tcgtatcgcc gcgcagcatg aagtcctgca
      841 gcagcctgat gccgtgcgtc acgggcaaga catacgagcc gtactggacc ccctccttga
      901 actcgtgcac cggcaggacg aatccggaga agaacaccga cgcgagcagc agcagcaggc
      961 acagctgcac cgcctgacgc tcggaatccg atacgaccga gatgatcagc ccgatgccaa
     1021 ggctggccag gatgatcagc gcaatcacca atccgaccat cagccagcta cccagcattg
     1081 gcacacccag cccgcccacc agcagggcga cgctgatcgc ggcgatcagc gcgctgactg
     1141 cgcccaggcc caggtacttg cccagcacga gctcaaacga gttgagcggc gagacgcgca
     1201 acagctcgag tgcgccggac atgcgctcgc gcacgaaaga gagcgcggtg agagtcacgg
     1261 ccatgtgctg gagcatcaac gccagcgcgg ccggagccgc gaagccgacg acgttgggct
     1321 gagtgggcgc gatgttggtg aggtgcacgg tggtcggcgc agcgatcacg tcgggcggca
     1381 cgcgcaccgg tggcgagacg tcttcgagca ggtacgactg gccctcgctc acggcctggc
     1441 gaatgatctc gcggttgatc tgctggtcga gcaggccggc caggaagctg gcgttgccgg
     1501 cctcgacggg atcgaccgag ttgaactcca cgtcgatctg cgcctggcgg ccggcgcgga
     1561 attgctccga ggcgtctgcc ggcgcgctga cgatgaggtc cagctgccgg gcggcgagct
     1621 gggcgtgcgc ggcgtcgggg gtctcatcaa cacggactat gtgcagcgcc gggccggcaa
     1681 gatcctggta gaacgccact tcgcgcggca aggacaggtt ggacgggatg acgaccaccg
     1741 tctcgagcgg ccgccggaca ccggtgtagc cgaggccgaa gacggccatg atcaggaacg
     1801 gcccaaggac caggctgaag aacgcgcccg gccggcggcg tacctggatg agctccttgc
     1861 cgacctgggc ccagacccga ccgatcgact tgaagggccc cagcagaagg tcggtcattc
     1921 gaagctcgct tgagcttcgg cgtccgattc cgcttccgcg cgcgcctcag catcgctcgt
     1981 gaccagtgcc gcaaacacct cgtcgaaggt gggccgatat tcgcggctgt agtcgacgtg
     2041 gccgccggct gcggcaaccg ccgcggcgac acgcgggctc gcgacaccgg cgtcgtgggc
     2101 gatcaccaac atctgtgcgg gtcccatctg acgcacacgg accacgccct cgatcggtgg
     2161 cagggagcgg gcatcgaacg gcgcgctcgt gccgacttcg atgacctcgc cgcccagggc
     2221 catgcgacgc aggtccgtcg gcgtggcatg ggcgatgagc cggccgccgg agatcagggc
     2281 cacggcatcg cagtactcgg cctccgtcac gtactgcgtc gtgaccagca ccgtcgcgcc
     2341 ctcgcgccgc tgatggtcga gctcgtgcca gatgcgcgtt cgcagcaagg ggtcgatgcc
     2401 cgccgtgggc tcgtcgagga cgagcagctg cggcttatgg acgagcgcac aggcgagctc
     2461 gaggcggcgc tgcatgccac cggacaggtc tgatgcgcgg cggtcacgag cctctcccag
     2521 gtcaacgagc tccagcacct ccgccacccg gcgaccgcgg cgcagcggcc cgatcccgaa
     2581 gagcgcggcc atgtgattga cgttctctcg cgcggtcagc tccgggtaga gcacgaacga
     2641 ctggggcatg tagccgatgc tctcgcgggt ccggcggtgg aagtggcgcg ggtcctcgcc
     2701 cagcacgcgg atgaccccgc ggtcaggttt gagggcgccg agcatgagcc ggatggtcgt
     2761 ggtcttgccc gagccgctcg gcccgatgac gccgagcagg ctgcccggcg cggccatcag
     2821 cgagaggtcg tcgacggcca ggaccttgcc gaagctgcgg ctgacgtggt ccaggacgac
     2881 cggcggcggg ccgggcggcg cgggcatgcg actggcgcgg ggcatggcgc tggtcggcgg
     2941 cgtgcctccg ggcggcgcgc ccgcgagagg cgcgcctgtt ggcggctgcg gccccccaga
     3001 ggcgctcatc ggtatggtca cgacgggtcc tcgcggtgcg ggcgagagct cgacgccggc
     3061 ccaagcatct cacggcgata atcgggccgg cgccaagggc aaaggatcgg tgccgggtcg
     3121 gtttcagcgc gacatgctgg cgctcagcca gcgctgcaac tccatcgaaa cgccgcatgg
     3181 ctggtgggac atcgcgcgac cgcgccggcg cgggctagcg tccgggtcat ggccgatgag
     3241 gggcgcgatg cagccaccgc aagcgacggg gtggaggcgg cccgggccgc aggcctgcgc
     3301 tacgccgacc cggcggagcc gggcatcagg cgcatccgcc gcggccgcgg gttcagctac
     3361 cgcgatgctg ccgggcggct cgtaaaggac cctgcgacgc tcgcgcgcat tcgcgcgctg
     3421 gccatcccgc cggcgtggcg cgaggtgtgg atctgcgctt ccgcgcgcgg ccacattcag
     3481 gcgatgggcc gcgacgcgcg cgggcgacgg cagtaccgct atcacgccga ctggaccaag
     3541 gcgcgcgacc agaccaagtt cgagcgcagc atcgccttcg cccgcgccct cccccggctg
     3601 cggcgacgtg tcgcacgcga cctgcgtcgc ccgggcatgc cacgcgacaa ggtgctcgcg
     3661 gctgtcgtgc ggctgctcga ccggaccctg ctgcgcgtag gcaacgagcg ttacgcgcgc
     3721 gagaactcat cattcggtct gaccacgctg cgcaatcggc acgcccgccc ggacggccgc
     3781 ggcgggatgc gcctgtcgtt tcgcggcaag ggcgggaagc gcttcgaggt gggcctgagc
     3841 gaccgccgcc tcgtgcgcat catccgcgct tgccaggacc tgcccggtca gcggctgttc
     3901 gagtaccgcg acgaaaacgg cgatgagcgg ccggtcgagt cggaggacgt caacgactac
     3961 attcgcgccg tgaccggcga cgatttcacg gccaaggatt tccggacatg ggccgcgacg
     4021 gtcctcgcgg cgcaggagct gcggcgcaac gatgacctgc ccgcggtcgt gcgagccgtg
     4081 gcggagcagc tggggaacac acccgccgtc tgccggcgca gctacatcaa ccccgtcgtc
     4141 cttgtgccgg cagatccacg ccccaacgcc catgtcgccc cggtgcttac cgggccgtcc
     4201 ggcctgaaca gcgacgagcg ccacgtgctg gcgctgctac gccgagcgcg ttctgcggcg
     4261 gcgcgcgccg catgacctga cgctggatcg tccacaatcg agcaaggtgt atcgaggaca
     4321 accggcgcgc agccgtctgc gcaacggcag caacggcaac ggcaacgggc acgaccgcgg
     4381 caatggccac ggccggcgca acgggcgggg caaccccaac gtgcggcgaa tcgccatcgc
     4441 acgcgtgcgc tcgcgctccg cgcccccgaa tcggctgggc ctgtacgcgg tgctggccct
     4501 cgcgggggtg gtcggcttct tcggggcaat cggtggctcc attggactgg gcacgacgac
     4561 cgggctgtcg ttcatcgccc agatggaggc cgagctgccg ccggtctcga acttcgagca
     4621 gctcgacttc gcccagccga cggtcgtcta cgaccgcacc gggaccgtcg agttggcgcg
     4681 cttccagatc gagcggcggc gcgtggtcgc gtacgaggcc atccccaagg tcctgctcga
     4741 cgccacgatt gccgtcgagg accagacgtt ctgggacaac gagggttacg accccaacgc
     4801 catcctgtcg gcggcactgg aaagcctggc gggcgaaggc gaccgcggcg catcaacgat
     4861 cacccagcag ctggtccggc agcggctgct cgaccccgag ctggtcgccg atcctgaccg
     4921 gctgctcgag cgcaaggtca aggagatcct gctcgcgcgc aacctgacca gtgcctatcc
     4981 gggcgatgaa ggcaagcaac gcatcctgac cgcatacctc aaccagatct attacggcca
     5041 cgccgcgtac ggcatcgctg ccgcggccga ggtctacttc ggcgtgtccg acctcaagct
     5101 gctcaccccg gcccaggccg ccatccttgc tggtctgccc caggcgccca acagctatga
     5161 cctctacaag tgggctgaga cggacgcaaa cggccagctc gtggtgccgc tcgtgccgac
     5221 ggaggcgcat ccggagatcg ccacgccggt caagcggcgc aacaagatcc tggagaacct
     5281 cgagcgcggc gacggccact tcatacgact cacgccgcag cagctcgacc aggcgctcgc
     5341 cgagccgatc gtcctcaagc cgccaactcc gttcgagctc aaggcgccgc acttcgtctt
     5401 ctacatgaag aggcagctcg aggcgctcgt cgccgatcgc gcccccgtcg agcgcggcgg
     5461 ctacagggtg atcacgacgc tcgacatggg cgcgcaggcc ctggcggaga aatacgttgc
     5521 cgcgggcacg atcgtgccca acctgccgac tggccagatg gaggccgaga tcgacgccca
     5581 gggcctgggc gaggaccggc gctggatcga ggcgctccac ggcacggaca tccacaacgg
     5641 ctcgctcgtg gcgatggacg cgcggaccgg gcaggtgatc gcctacgtcg gcagcgccgg
     5701 ctactaccgc gacgacatcg cctcaccgca attcgatccc aagtacgacg tcgcggggtc
     5761 ggccttcagg cagcccgggt cggcgtggaa gccgatcatg tacgcggccg gattcgatca
     5821 acgcgtcgtc acgccgggca cgctcctcaa cgacgtgatc accgagttca gccgcgactg
     5881 gtacccgcgc gacgccgacc gcaaggagcg cgggccggtc ctgatgcgcg atgccttgac
     5941 gtactcgctc aacatcccgg ccatccgggc actggaccgc attggggtcg accccgttgc
     6001 cagcctcgcg agcgccatgg acctggactt cctgcgcgag aggcagctct tccaggcggg
     6061 cctcgccagc gccatcggca ccgtcgaggt gaagcaggtc gagctgaccg cggcctacgg
     6121 ggccctggcc aaccagggtg tctatgagcc gatccagacg atcctctcga tcaccgacgc
     6181 caacggcaac ccggtcccgg tcacgcccgc tgcgcccacc caggtcctga ccgaacaggc
     6241 cgcgtggctg atgagcgaca tcctcaagga ttcgaccgac ccggcgctca acacggtctt
     6301 cgggccgcgc ctgcagatca tcaacggcat ccgcgatccg ctggtcccgg gcagcgagcg
     6361 ccggccggcc gcagccaaga cgggtaccgc caacgacctg cgcgacctgc tcgcgttcgg
     6421 gtatttggga ccgccggctg atcccaacgc gatgcacatc gtcgcgtcgg tctggacggg
     6481 caacagcgat cactccgcgc cgaacgccgg cgacttctcg atcatcgccg ccgacggacc
     6541 ggggcgcatc tggtcgtcgt tcctgcgcga gctctcgcgc gactggccca tggccacgtt
     6601 cccggcgcca ccgccaggcg tcgtccaggc gacgatcgat gcctggtccg gcggcgcgcc
     6661 gggtccgtgg acgcgcgata cgcggaccga gtggttcatc gagggcacgc agccgggcgg
     6721 ccagttccag gtcgacccgc cgggcctcct ctacacccag atgtgcggcc gctggttcgt
     6781 cgacatcgcg aaggcggagg cggccgaccc gccgcgctgg atcgaggcgg acctcggctg
     6841 gatggagcgc gcgcggcgcg gcacgggcgt gcgcggcgag aacggcggca ccaccgcgca
     6901 cctctttggt cgctcggact ggggcggctt catcgcgccc attgattgcg catccgcgcc
     6961 ccagccaacc ccgcagccac cacccgatca cacgcccggc ccgggaccca cgccgccgcc
     7021 cgggccgacg ccgccgccag gcactcccaa gccgaccgac ccgccaccga cggaaccgcc
     7081 gccgactgct gccccctaac ccgggccacg cgatccccgc cccccgtccg ccatccccac
     7141 cgcacctgtc atcccagcag aagtgctcac ctgtcagcct taagccgcgc aggagagctc
     7201 ggcgaggcgc gaccgcgtcg gtgctgagcg tggtcgtggg cggggcgagc gctgtgggcg
     7261 aggccgcacg cgcgtaaggt cgaccagcgg gtacacctgc cgggatggtc gcggcatgcc
     7321 gggataacgg cgcgccaagc cggcgataac ccgcgatccc gacactgcca gcgtgatcaa
     7381 tcgccgcgtc ggccgcatcc atcgcgcgct ccgccagcgg gcacacctaa cgcagcgtga
     7441 gctgagcgcc aactcgggtg tcgtccgctg gaaaatcggg cgaatcgagc gtgacctggt
     7501 cgaccgcttg cgtttcgacg aggtcgaacg ttgcctggcc gcgctggggg cggagctcga
     7561 ggtgcgcgtc agttaccgcg gtgcggaggt ggatcggctc atagatcggg tccactcgct
     7621 ggtgggcgcg cgagttatcg aggtcctgcg tcggcaaggc tgggaggtgc agacggaggt
     7681 cacgttctcg gagtggggtg agcgcgggtc gtacgacatc ctggcctggc acccccaatc
     7741 gcgaacgctg ctggtgatcg agatcaagtc cgagttggcg tcagtcgaag gcacgcttcg
     7801 tcagctcgat gtcaaggttc gccttgcccg atcggtagcc cgcaagcggt ttggctggga
     7861 ggcgcgggcc atcgccaggg tcatggttct gccggaagac cggagcgctc ggcggcaggt
     7921 agcggccaac gccgcgctct tgcgggctgt gctgcccgcg acgtcgcgcc aggtccgcac
     7981 gtggctccgt ttcccggacc gcagcctgtc cggtatctgg ttcctgtccg ccgcggacgg
     8041 acggccgtgg ccgcgcaatc cgtcttcgat ccggcgtgtc cgatcgaagg ccgccgcggg
     8101 cgcgacgcgt tagatcgtcg cgagcgggac gcacctgaaa gcaggctgat acggcgcgcg
     8161 ggttcgcgcc ggaatgtcag cgagcggcgc tggtggacca tccggtcatc cacacagcgg
     8221 agggactcac catggctcag acagggacgc gctcgaccac gacgcgcaag agcaccagca
     8281 ccgacaccga caacaacgag aacgggaccg ggcagactca gatcaatgcg gtcgtgagcg
     8341 gcgcgcagca agcggccgag gtggctcgcc agacggcgga gcgcgccgcg gcgcggttgc
     8401 ccgatgccgt cgcgcggaca caggtcgcgg cgcgcgacac gcaggtcgcc ctcgagggca
     8461 tggacggcca gacccttttg gcggggacga cgttctcgct gggcttgggc gttggcctct
     8521 tcgtcagcgg cgcgcaccga ctgttggtga cggcggcgtt cctgccggcg ctggcgatgc
     8581 tgcttacgct cctgggccgc cagcgcgaag aggcatagcc agcgcggggt aactcgcggg
     8641 ccaagctgaa cgcggcgcga gcgtcgagag cgccgagagc cccgaaaggg gctaggccgt
     8701 taccgcggtg ccggtggccg ggccggtcga gccggtcgct gtgccgccgc catggctggt
     8761 gctcgagtcg ccgaggttgc cctcagtgtc cagcacgtag ccgatgccct ggaacgtctt
     8821 gatggggccg gcctcgccgg gc
//
LOCUS       DATBRX010000043        10278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2583728, whole genome shotgun sequence.
ACCESSION   DATBRX010000043 DATBRX010000000
VERSION     DATBRX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583728"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VIF08_01475"
     CDS             <1..358
                     /locus_tag="VIF08_01475"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEY8134680.1"
                     /translation="PDCPPVAMISRPLGIQTNNYAEYTGVILALEKALAMGAREVELV
                     LDSMLIVEQLSGRWKIKHPLIRPLAGRAMELLRQFDRWSLRHERRENNVQADALANLA
                     LDDPAAAAAMERGGAL"
     gene            355..858
                     /locus_tag="VIF08_01480"
     CDS             355..858
                     /locus_tag="VIF08_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8134681.1"
                     /translation="MTEPVVSLEPWSAADRPLLDALLGVPEMMEYLGGPESKDKLDER
                     QARYVNNPRSLRIVVDGEGVGWLGYWDHEEADGSLAWETGWSVRREFQGRGIATAAMR
                     LAFEQMRRDGNRRYVHATTTVDNAPSNALCRKLGFELVGPIDYEYPPGNPVHGNDWRL
                     DLKADQR"
     gene            855..1376
                     /locus_tag="VIF08_01485"
     CDS             855..1376
                     /locus_tag="VIF08_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8134682.1"
                     /translation="MSEIRIEPWGSGDLALMRRLLGDPVMTEHLGGPESEQKIAERQA
                     RYEKPDSRQYKVVVGDVHAGAVGYWEKEWRGTTVWEVGWLVLPEHQGRGLATAGMRLL
                     LEVIKADTVQYQFVHAFPSVDNAPSNSICRKLGFTLLEAIDFEYPPGSGSVLHCNDWR
                     LDLTGGGALGDPG"
     gene            1391..1737
                     /gene="rnpB"
                     /locus_tag="VIF08_01490"
     ncRNA           1391..1737
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIF08_01490"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1872..2132)
                     /locus_tag="VIF08_01495"
     CDS             complement(1872..2132)
                     /locus_tag="VIF08_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134683.1"
                     /translation="MAIQTRPLPPQSGMQPQDASAPAPLRRCTFRRVALKAAAGPRGL
                     PMYDVACTFPDRRKAVPLGDIEDARPVCASCTYQGIFRADSD"
     gene            2218..2895
                     /locus_tag="VIF08_01500"
     CDS             2218..2895
                     /locus_tag="VIF08_01500"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY8134684.1"
                     /translation="MNEWGSSSRYGLVDVALYLLGIAGLAASLTLVFLSMRAVMDVGG
                     FCAEGGPYEIQTHCPEGTVVLLPLSIFAGLGSAALAGWKGSQIGGPFAGLVALAWPAL
                     FISLGWNFLEYALWPPGDQGGIVWGWLIPGIVFEIMGILPLLGLLPSRRAFLGTETAD
                     SPVALTSARKRLLNDLVSHAHERGWGAQEDLVSKLERLAALREQGAITSAEFEAAKQA
                     VIGGSTR"
     gene            2892..3014
                     /locus_tag="VIF08_01505"
     CDS             2892..3014
                     /locus_tag="VIF08_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134685.1"
                     /translation="MTIDDNGGPFSTQILLLLVVVAVFAGILFAMWLFGVMTGT"
     gene            3409..3834
                     /gene="mraZ"
                     /locus_tag="VIF08_01510"
     CDS             3409..3834
                     /gene="mraZ"
                     /locus_tag="VIF08_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY8134686.1"
                     /translation="MFTGEFRHSIDGKGRVAVPARFRADLAAGATVCRWLENCVAIFP
                     KAAWQTLAEAASAERYADAGARLFTRFLFSGAFEVELDGQGRIVLPASLRHFGGLKSD
                     AVVVGAADHIELWEPARWDGISAEMSSEEFAERIGGLGI"
     gene            3845..4831
                     /gene="rsmH"
                     /locus_tag="VIF08_01515"
     CDS             3845..4831
                     /gene="rsmH"
                     /locus_tag="VIF08_01515"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY8134687.1"
                     /translation="MSLQGQQGPPLADAVTEGHLPVMVQEVLESLAPAPGSFQIDATV
                     GGGGHSRPILEATSPDGRLLGLDADRAAIARTRERLADLGARLTLRQANFEELADVAD
                     DAGFNPADGILFDLGLSSYQLADDARAFSFRATGPLDMRFDTGRGVPASTLINELDED
                     ALTDLFKRLGEEPQARRIARAVVARRGREPISTGQQLADIVAAAAPAPPRGRRRVHPA
                     TRVFQALRIAVNRELETLPVALAAAVGILRTNGRLAVISYHSLEDRIVKRFIAAERRG
                     CICPPESPVCVCGRQPRLALVGAQPRRPSDAEVARNPRSRSALLRAARRLAA"
     gene            4851..5051
                     /locus_tag="VIF08_01520"
     CDS             4851..5051
                     /locus_tag="VIF08_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134688.1"
                     /translation="MSRRNRANRRRTYGKRQHEVRERRPGDSPQGDGENPGENWITPE
                     LMDDRGRREATYDAYDTEGWAR"
     gene            5048..5404
                     /locus_tag="VIF08_01525"
     CDS             5048..5404
                     /locus_tag="VIF08_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134689.1"
                     /translation="MTATYVERPWRGARPAPTTRRRVSRPDRAGRRVHPVGALLAVIL
                     VAFLLGLTYLAQTVQLAATEYQIVQLQVQSDDLHRQIQTVETGVLHWGAESTVLENAQ
                     GLGLDQLPVGARLIER"
     gene            5408..7249
                     /locus_tag="VIF08_01530"
     CDS             5408..7249
                     /locus_tag="VIF08_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8134690.1"
                     /translation="MLGRTDSRLRLVALLGIFALIASLLGLRLAYWQIGQSDMLRGLA
                     AAQVLRPGEGRAIERGQILDRGGNVLATTAYRDLLAAYPDLMSDEQRETIPARLAEIL
                     NMSDQQTTELTARFTADRPYVIVARQLTEAQSAAVRAGLADESLAQLDLEPHPVRFYP
                     NAGGSPNTTLASQLLGFVNQEGLGRYGIEQSSQDVLSGQSGAVAAINGSTTTATEGGS
                     VQLTIDASLQLRLEKELYAAWVADRATRVTGAVIDPYTGAILAWASVPGYDANDYSEV
                     AEKSPELFSDPVYSQVYEPGSVMKMLTAAAALEDGVVTLDSPVLDDARLHLGSNIVEN
                     FDKKGMGIIPFEDVIANSRNVGTGHVALALGDTVADASGRLFQMWQRLGIGEMTGVGL
                     QAESAGIVPDPAGYPWQSIDLVNRSFGQGVAVTPLQLARAFSAMVNGGQLPSLHVYSS
                     IDGEPVTVPDPTQVISPELSATLLQLMIHVVDAGPHYAEETLIPGYVVGGKTGTAQIW
                     DTRNDQWMPHIYNHSFVGFVGAERPEAVIVVRIHEAQPEGRGRDQYRIELTSNELFRR
                     VALDTIDVLDIPPLPAGVPQQTAEPSVGVPAQEPEVDATAPPMAAGG"
     gene            7276..8730
                     /gene="murF"
                     /locus_tag="VIF08_01535"
     CDS             7276..8730
                     /gene="murF"
                     /locus_tag="VIF08_01535"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY8134691.1"
                     /translation="MTDRNDPSSLAFDARSLTGAISGELLHAGSAPIRGGAVDSRKVE
                     PSNAFFALPGERTDGHNFLADAAHRGAAALVVARRLGGAALRAIAEAGAPSVVLVPDV
                     LAALHAAAAAWRARFEPVVVGVTGSVAKTSTKEQVAQVLAQRWTVLRNQANENNEIGL
                     PLTLLRLAPTDDVAVLEMGMYKPTDIALLAELAQPRVGVVTAVRPTHLARAGSIEAIE
                     RGKRELVDALPVGGTAVLNADDERVARMADGRKGQLRVVRYGFGASADVGATNVESLG
                     EEGMRFDLRLPGGTAPARIPALGRHSVHNALAAAAVGDALGLDAAMIVAGLAQQFSVP
                     HRTTLVRAGDWRILDDTYNAAPDSMAAALDLLASLPGRRVAVLGEMLELGDLSDEAHR
                     KVGAHAARAAQLLVAVGPFAALYAAGARAAGMDAAAIVETPDRAATVAHLERRLRPGD
                     IVLVKASRGAELDLLVDELRRLAEHDSATPEPVA"
     gene            8727..9716
                     /gene="mraY"
                     /locus_tag="VIF08_01540"
     CDS             8727..9716
                     /gene="mraY"
                     /locus_tag="VIF08_01540"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8134692.1"
                     /translation="MTITAAVVQGILLTFALMVILMPSFITLLRRAGFGKRIREEGPA
                     THYIKEGTPTMGGVLMIAVVGVSALLFHFVGGGIFLGPQTIAPLLTLLLVGFLGAADD
                     YLNATTGEGIRIRQKLLWQIVVAGVIAYQIQNTYPSATTLAVPFLGSVAIDPIAYIAF
                     AGFAIVATTNGVNFTDGLDGLAGGTLIFAFVAYMIIALLGGQQELAILCALLIGALLG
                     FLWFNVHPAQVFMGDAGSLSMGATLAVISLITGQILLLPLIGIIFVLQVGSDVIQIGY
                     YKMTHKRIFKMAPLHHHFELSGWDEEKITMRFWIVSVLAAMLGVTFFFSTLGR"
     gene            9713..>10278
                     /locus_tag="VIF08_01545"
     CDS             9713..>10278
                     /locus_tag="VIF08_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134693.1"
                     /translation="MSGAAVVVEPIDLDALTLDSFRGRPVAVLGLARSGIALCRFLAD
                     RGAVVTGYDARPVDELSALDRLEGRPVRLLLGPDVEPRVALADQALICTSPSVSSHYP
                     TTEPRLRSALAEIEAAGVVPVVSEVDLFLRLCPATTIGVTGTKGKTTTSSLTAAVLAA
                     GPAPVVLGGNIGVPLVERLPELTADHRVV"
BASE COUNT         1580 a         3560 c         3536 g         1602 t
ORIGIN      
        1 tcccgactgc ccgcccgttg ccatgatcag ccggccgctg ggcatccaga ccaataacta
       61 cgccgagtac acgggcgtca tcctggccct ggagaaggcg ctggccatgg gcgcgcgcga
      121 ggtcgagctc gtcctggatt cgatgctgat cgtcgagcag ctgagcggcc gctggaagat
      181 caagcatccc ctgatacgtc cgctcgccgg gcgggcgatg gagctgctgc gccagttcga
      241 ccgctggtcg ctgcgccacg agcggcgcga gaacaacgtc caggccgacg cattggcgaa
      301 tctcgcgctc gacgatcccg ccgcggccgc cgcgatggag cgagggggcg ccctgtgacc
      361 gagcccgtcg tttcgctgga gccatggagc gccgcggacc ggccgctgct cgacgcgttg
      421 ctgggcgtgc cagagatgat ggagtacctg ggcgggccag agagcaagga caagctcgac
      481 gagcgccagg cgcgttacgt caacaacccg cgcagcctgc gcatcgtggt cgacggcgag
      541 ggtgtcggct ggctcggcta ctgggatcac gaggaggcgg acggctcgct cgcctgggag
      601 accggctgga gcgtgcgccg cgagttccag ggtcgcggca tcgccacggc ggcgatgcga
      661 ttggcattcg agcagatgcg ccgcgacggc aatcgacgct acgtccacgc caccacgacg
      721 gtcgacaacg cgccttccaa cgcgctctgc cgcaaactcg gctttgagct cgtcggaccg
      781 atcgactatg aatatccgcc ggggaaccct gttcacggca atgactggcg gttagatctc
      841 aaggccgatc agcgatgagt gaaatccgga tcgagccgtg ggggagcggc gacctggcgc
      901 tcatgcgccg actgctcgga gatcccgtta tgaccgagca cctgggcggc ccggagagcg
      961 agcagaagat cgccgaacgt caggcgcgat acgagaaacc tgattcgcgg cagtacaaag
     1021 tcgtggtcgg cgacgtgcac gccggcgcgg tcggctactg ggagaaggag tggcgcggca
     1081 cgaccgtgtg ggaggtcggg tggctcgtgc tgcccgagca tcagggccgc gggctcgcta
     1141 ccgcggggat gcgcctgctg ctcgaggtaa tcaaagccga cacagtccaa taccagttcg
     1201 tgcacgcctt tccatcggtc gacaacgcac cgtccaactc gatctgccga aaactcggct
     1261 tcacgctgct tgaggcgatc gacttcgagt acccacctgg cagcggcagc gtgctgcact
     1321 gcaacgactg gcggctcgac ctgactgggg gcggagcgct gggcgacccg gggtagaatc
     1381 agggggccag cgagatggtc ggacggtcgc gtgtcccgtc ggcgcaagcc gttaccgaca
     1441 cgaggaaagt ccgagctccg cgacgcaggg tagcgggtaa ctcccgtccg ccgagaggcg
     1501 aggaccagcg ccacagagac gagccggcag cgggcaaccg cgggccggag tgaaacgggc
     1561 aagctctacc cggagcaagg ccaaataggg gagcggtcgg acgcaagttc gaggcgagcg
     1621 gcgtcgctcg gctcccgggt cggccgctag agacggcggg taaccgtcgt cgtagatgga
     1681 tgaccgtccc cgatcgcaag atcgggacag aactcggctt acagaccgtc tcgctggcaa
     1741 gcccgcaggc cagcgcctgc atctgcaggg gcaagccaag ggcgcgcagc agcggcccgg
     1801 cggggccgac tgcggagacg aaatcagtta cacccgaaga ccggcacccg cgttgcgcgg
     1861 gcaacccgcg actagtcgga gtcggcccgg aagatcccct ggtaggtgca cgacgcgcac
     1921 accgggcggg cgtcctcgat atcacccagc ggcacggcct tgcgccgatc ggggaacgtg
     1981 cacgcgacgt cgtacatcgg cagtccgcgc ggaccggccg cggccttcag ggccacgcga
     2041 cggaacgtgc agcggcgcag cggcgcaggc gcactcgcat cctgcggctg catgcccgac
     2101 tgcgggggca gcggcctggt ctggatcgcc atgcccgcac cctaggcaaa tcgacgtccc
     2161 gtcacaaggc aaccagcagt actacgccgt gagtcgcggg tggttaccat cgcgagcgtg
     2221 aacgagtggg gttcgagctc gcgttacggc ctggttgacg tcgctctgta cctgctcggg
     2281 atcgccggcc tcgcggcctc cctgacgctg gttttcttga gcatgcgcgc ggtgatggat
     2341 gtcggcggct tctgcgccga gggtggcccg tacgagatcc aaacgcactg ccccgaaggc
     2401 accgtcgtgc tcctgccgct gtcgatcttt gcgggcctgg gatccgcggc cttggctggc
     2461 tggaagggat cgcagatcgg cggcccattt gctggcctgg tggcgctggc ctggccggcg
     2521 cttttcatca gccttggttg gaacttcctc gagtacgccc tctggccgcc cggcgaccag
     2581 ggcgggatcg tctggggctg gctcattccg ggcatcgtct tcgagatcat gggcatcctg
     2641 cccttgctgg ggctgctgcc cagccggcgc gcgtttctcg ggacggagac ggccgattca
     2701 ccggttgcgt tgacgtcggc ccgcaagcga ctgctgaacg acctcgtgtc gcacgcccac
     2761 gaacgcggct ggggcgcgca ggaagacctc gtgtcgaagc tcgagcgact ggccgcgctg
     2821 cgcgaacagg gtgcgatcac cagcgccgag ttcgaggccg cgaaacaagc agtcatcggc
     2881 gggtccacgc gatgaccatc gacgacaacg gcggcccatt ctcgacgcag atcctgctcc
     2941 tgctcgtggt cgtggccgtg ttcgcgggca tcctctttgc catgtggctt ttcggagtga
     3001 tgaccggcac ctgatcgctc cgcatcccgc gtcgtggtgc gctcgacgca cctagcgtcc
     3061 ggtcaaaccc ggtccagagg catctgactg cgctggtcgt tcgcccggtg cgcctcctgc
     3121 accagcgagc gtctggagcg cgccgtcggc ccggacctgg gttgccggac ggacgatagg
     3181 tgcgctgcat gcaccggccc gggcgccgat cccactacgt gttggattgg ggcttgacgt
     3241 ggcttcggcg tgcactatga tcccacgaag tggcccagag tgggtcaaag tggggagaga
     3301 gacatagaca gtcccgcccg ggcacgcgtt ttggcacgaa ttcgcacgcc cgggtggtga
     3361 caaacctctt cgagccgcgc tcctgtccag cgaccacgag gtagcggtgt gttcaccggc
     3421 gagttccggc attcaatcga cggcaagggc cgggtagccg tgcccgcgcg tttccgagcc
     3481 gacctggccg ccggcgcgac ggtctgtcgc tggctggaga actgcgtcgc gatcttcccc
     3541 aaggccgcgt ggcagaccct tgccgaagcg gccagcgccg agcgctacgc cgatgccggc
     3601 gcacgtctgt tcacgcgctt ccttttctcg ggcgctttcg aagtagagct cgacggccag
     3661 ggccggatcg tcctgcccgc cagcctgcgc cacttcggtg gcctcaagtc ggatgcagtc
     3721 gtcgtcggtg cggcggacca catcgagctg tgggagcccg cgcgctggga cggcatcagc
     3781 gccgagatga gctccgagga gttcgccgag cgcatcggcg gcctcgggat ctaggcgcca
     3841 ccaaatgagc ctccagggtc agcagggacc accgttggcg gatgccgtga cggaagggca
     3901 cctgcccgtg atggtgcaag aggtactgga atccctcgca cccgctccgg gcagcttcca
     3961 gatagacgcg accgtcggcg gtggtgggca ctcccggccg atcctggagg ccacctctcc
     4021 tgatggccgc ctgctcggcc tcgacgccga tcgggcggcc atcgccagaa cgcgagaacg
     4081 gctggccgac ttaggcgcac ggctgacgct gcgccaggcg aacttcgaag agctggccga
     4141 cgtggccgac gacgccggct tcaacccggc cgacgggatc ctcttcgacc tggggctgtc
     4201 gtcgtaccaa ctggccgacg acgcgcgcgc gttctccttt cgcgcgacag gtccgcttga
     4261 catgcgcttc gacaccgggc gcggcgtgcc tgcctcgacg ttgatcaacg agctcgacga
     4321 ggacgcgctg acggacctgt tcaaacgcct cggcgaggag ccgcaggcac gccgcatcgc
     4381 gcgcgccgtg gtcgcgcggc gcggtcgcga accaatatcg actggccaac agctcgcaga
     4441 catcgtggcc gccgccgccc cagcccctcc cagggggcgg cggcgagtcc accccgcgac
     4501 gcgcgtcttc caggcgttgc gcatcgcggt caaccgcgag ctcgagacac tccccgtggc
     4561 gcttgccgcg gccgtcggga tcctgcgcac caatggccgg ctcgcggtca tctcgtacca
     4621 ctcgctcgag gaccgcatcg tcaagcgctt cattgcggcc gaacgacggg gctgcatctg
     4681 cccgcccgaa tcgccggtat gcgtctgcgg ccgccagccg cggctcgcgc ttgttggcgc
     4741 ccagccgcgc cggccgagcg acgccgaagt ggcacgcaac ccgcgctcgc gcagcgccct
     4801 gctgcgcgcc gcacggcggc tcgcggcatg agcccggagc gacgacagcg gtgagtagac
     4861 gtaaccgcgc caacaggcgg cgcacctacg gcaaacgcca gcacgaagtg cgcgagcgcc
     4921 gcccgggcga ctcgccgcag ggtgacgggg agaaccccgg agagaactgg atcacgcccg
     4981 agctgatgga cgaccggggg cggcgcgagg ccacctatga cgcctacgac acggagggct
     5041 gggcgcgatg acagccacct acgtcgaacg gccgtggcgc ggcgcacgac ccgcgcccac
     5101 cacccgccgc cgcgtgtcac gaccggatcg cgccggccgg cgcgtccacc cggtcggggc
     5161 gctgctggcc gtcatcctgg tcgccttcct gctgggtctg acgtatctcg cgcagaccgt
     5221 ccagctcgcg gcgaccgaat accagatcgt gcagctgcag gtgcagagcg acgacctgca
     5281 ccgtcagatc cagacggtcg agacgggcgt ccttcactgg ggcgccgagt cgaccgtgct
     5341 tgaaaacgcg cagggtctgg gcctggacca gctcccagtc ggggctcgcc tgatcgagag
     5401 atgagcgatg ctcggacgaa ctgacagccg tctgcggctg gtcgcgctgc tgggcatctt
     5461 tgcgctcatc gcgtcgctgc tgggtctgcg cctggcctac tggcagatcg gccagtcgga
     5521 catgctgcgc ggactagccg ccgcccaggt cctgcgaccc ggcgaggggc gggccatcga
     5581 gcgcggccag atccttgacc gtggcggcaa tgtcctcgcc acgacggcct atcgcgacct
     5641 gctggccgcg taccccgacc tgatgagcga cgagcagcgc gagaccattc ccgcgcgact
     5701 ggccgagatc ctcaacatgt ctgaccagca gaccaccgag ctcaccgcgc ggttcaccgc
     5761 cgaccggccg tacgtaatcg tggcgcgaca gctgaccgag gcccagagcg ccgccgtgcg
     5821 cgcaggcctc gccgacgagt cgctcgcgca gcttgacctg gagccgcacc cggtgcgctt
     5881 ctaccccaat gccggcggtt cgccgaacac gacgctcgcg agccagttgc tgggcttcgt
     5941 caaccaggaa ggcctgggtc gctacggcat tgagcaatcg agccaggacg tgctgtcagg
     6001 gcagagcggc gcggttgccg cgatcaatgg cagcaccacg accgcgaccg agggcggcag
     6061 cgtgcagctg accatcgatg ccagtcttca gctgcgcctc gagaaggagc tctacgccgc
     6121 gtgggtggcc gaccgcgcca cgcgcgtgac gggcgccgtg atcgacccct acacgggcgc
     6181 gatcctggcc tgggcgagcg tgcccggata tgacgccaac gattactccg aggtcgccga
     6241 gaaatcgccc gagctgttct ccgacccggt ctacagccag gtctacgagc ccggatccgt
     6301 catgaagatg ctgaccgccg cggcggcgct ggaggacggc gtggtcaccc ttgactcgcc
     6361 ggtgctcgac gacgcgcgcc tgcacctggg ctcgaacatc gtcgagaact tcgacaagaa
     6421 gggcatgggc atcatcccct tcgaggatgt catcgccaat tcgcgcaacg tcggcaccgg
     6481 ccacgttgcg ctggccctgg gcgatacggt cgctgacgca tcggggcggc tgttccagat
     6541 gtggcagcgc ctgggcattg gcgagatgac cggcgtcggg ctgcaggccg aatccgccgg
     6601 catcgtcccc gatccggccg gctatccgtg gcaatccatc gacctggtga accgatcctt
     6661 cggccagggc gtggccgtga cgccgctcca gctcgcgcgc gccttctcgg cgatggtcaa
     6721 cggcggccag ctgccgtcgc tccacgtcta ctcgtccatc gatggcgagc cggtaacggt
     6781 gcccgacccg actcaggtca tatcacctga gctgtcagcc accctgctgc agctgatgat
     6841 ccacgtcgtt gacgcgggtc cgcactacgc cgaggagacg ctcatcccgg gctacgtcgt
     6901 tggcggcaaa accggcaccg ctcagatctg ggacacgcgc aacgaccagt ggatgccgca
     6961 catctacaac cactcgttcg tgggcttcgt cggcgcggag cggcccgagg cggtgatcgt
     7021 cgtgcgtatc cacgaggctc agcccgaggg ccgcggccgc gatcaatacc ggatcgagct
     7081 cacctcgaac gagctcttcc ggcgggtcgc gctggacacg atcgacgtgc tcgacatccc
     7141 gccgctcccc gccggggtgc cgcagcagac ggcggagccg agcgttggcg tgccggccca
     7201 ggagcccgaa gtcgatgcca ctgcgccgcc catggcggcc ggtgggtagg gcgcatcgtg
     7261 cgagaattcc cgcccttgac cgaccgtaac gatccatctt cgctcgcgtt cgacgcgcgc
     7321 tcgctgacgg gcgcgatctc tggtgagctg cttcacgcag gcagtgcgcc aatccgtggc
     7381 ggcgcggtcg attcgcgcaa ggtcgagccg agcaacgcct tcttcgcgct gccgggcgag
     7441 cgcaccgacg gccacaactt cctggctgac gcagcgcacc gtggcgcggc cgcgctcgtc
     7501 gtcgcgcgcc ggcttggcgg tgccgcgctg cgcgccatcg ccgaggccgg cgcgcccagc
     7561 gtggtcctcg tgcccgacgt cctggccgcg cttcatgccg ccgccgctgc gtggcgcgcc
     7621 cgctttgagc ccgtcgtcgt gggcgtcacc gggtcggtcg ccaagacgtc aaccaaggaa
     7681 caggtggcac aagtgctggc gcagcgctgg acggtgttgc gcaaccaggc caacgagaac
     7741 aacgagatcg ggcttccgct gacgctgctg cggctggcac cgaccgatga cgttgccgtg
     7801 ctcgagatgg gcatgtacaa gccgacagac atcgcgctcc tggccgagct cgcccagccg
     7861 cgggtcggcg tcgtcaccgc cgtgcgcccg acacacctcg cccgcgcggg ctcgatcgag
     7921 gccatcgagc gcggcaagcg cgagctcgtt gatgcgctgc ccgtgggcgg cacggcggtg
     7981 ctcaacgccg atgacgagcg cgtggcgcgc atggccgacg gccgcaaggg ccagctgcgc
     8041 gtcgtccgct acggcttcgg cgcgtccgcg gatgtgggcg cgaccaacgt cgagtcgctc
     8101 ggcgaggagg gcatgcgctt cgacctgcgc ttgcccgggg gcaccgcgcc ggcgcgcatc
     8161 ccggcgctcg gccggcacag cgtccacaac gccctggccg cggccgccgt tggtgacgcg
     8221 ctggggctcg atgcggcgat gatcgtcgcc ggtctcgccc agcagttcag cgtgccccat
     8281 cggacgacgc tcgtgcgcgc gggcgactgg cgcatcctcg atgacaccta caacgccgcg
     8341 cccgattcca tggccgcggc gctcgatctc ctggcgagcc tgcccgggcg gcgagtcgcg
     8401 gtcctgggcg agatgctcga gctgggcgat ctatctgacg aggcacaccg caaggtaggt
     8461 gcgcacgccg cccgggccgc gcagctgctg gtcgcggtcg ggccgtttgc ggcgctctac
     8521 gcagccggcg cgcgcgcggc cggcatggac gccgcagcca ttgtcgagac gcctgaccga
     8581 gcggccacag tggctcacct ggaacgccgg ctgcgtcccg gcgacatcgt gctggtcaag
     8641 gcatcacgcg gcgctgagct ggacctgctg gtcgacgagc tgcgccgact ggccgagcac
     8701 gactcggcca ctccggagcc cgtcgcatga ccatcaccgc ggcggtcgtc cagggcatcc
     8761 tcctgacctt cgcgttgatg gtcatcctga tgccgtcgtt catcacgctg ctgcgccggg
     8821 ccgggttcgg caagcgcatc cgcgaggaag gtcccgcgac tcattacatc aaggaaggca
     8881 cgcccacgat gggcggcgtg ctgatgatcg cggtcgtcgg cgtcagcgcg ttgctcttcc
     8941 acttcgtcgg cggcggcatc ttccttggcc cgcagaccat cgcaccgctg ctgacgctgc
     9001 tgctcgtcgg cttcctgggc gccgccgacg actacctgaa cgccacgacc ggcgagggca
     9061 tccgcattcg ccagaagctg ctgtggcaga tcgtggtcgc gggtgtgatc gcctaccaga
     9121 tccagaacac ctatccatcc gcgaccactc tggccgtgcc cttcctgggc agcgtggcga
     9181 tcgatcccat cgcctacatc gcgttcgccg ggttcgcgat cgtggccacg accaacggcg
     9241 tcaacttcac cgacgggctc gacggcctgg ccggcggcac gctgatcttc gcgttcgtcg
     9301 cctacatgat catcgccctg ctgggcgggc agcaggagct ggccatcctg tgcgcgctgt
     9361 tgatcggtgc cctgctcggc ttcctttggt tcaacgtgca tccggcgcag gtcttcatgg
     9421 gcgatgccgg ctcactctcg atgggcgcca cccttgccgt catctcgctc atcaccggcc
     9481 agatcctgct gctgccgttg atcgggatca tcttcgtgct ccaggtcggg tcggacgtga
     9541 tccagatcgg ctactacaag atgacccaca agcgcatctt caagatggcg ccgctgcatc
     9601 accacttcga gctgtcgggc tgggacgaag agaagatcac gatgcgcttc tggatcgtca
     9661 gcgtgctcgc ggcgatgctc ggcgtgacgt tcttcttctc gacgctgggc cggtgagcgg
     9721 ggccgcggtg gtcgtcgagc cgatcgacct cgacgcgctg actctcgatt ccttccgcgg
     9781 ccggccggtc gcggtgcttg gcctggcccg cagcggcatc gcgctgtgcc gcttcctcgc
     9841 cgatcgcggc gccgtggtga cgggctacga cgcgcgcccg gtggatgagc tgtccgcact
     9901 cgaccggctc gaagggcggc cggtgcgcct gctgctgggc ccggacgtcg agccacgcgt
     9961 ggctctggcc gatcaggcgc tcatctgcac ctcgccgtcg gtcagcagcc actacccgac
    10021 gacggagcca cggctgcgct cggcgctcgc cgagattgag gccgcaggtg tcgtgccggt
    10081 ggtcagcgag gtcgaccttt tcctgcgcct gtgtcccgcg acgaccatcg gcgtgaccgg
    10141 caccaagggc aagacgacga cctcgtcgct gaccgcggca gtgctcgccg ccggtccggc
    10201 gccggtggtg ctgggcggca atatcggcgt gccgctggtc gagcggctgc ccgagctcac
    10261 tgccgatcac cgcgtggt
//
LOCUS       DATBRX010000044         5327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1466403, whole genome shotgun sequence.
ACCESSION   DATBRX010000044 DATBRX010000000
VERSION     DATBRX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466403"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4098
                     /gene="rpoC"
                     /locus_tag="VIF08_01550"
     CDS             <1..4098
                     /gene="rpoC"
                     /locus_tag="VIF08_01550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY8134694.1"
                     /translation="DELKEGLKTLKAGAEEERTARTAELVEKQQASEATMKALGKAAA
                     EEPIVFEPTGEVIVAAGDKADKPAFTRLNKAAAAEVERVNDEIKGREKNEDDSVKRQI
                     EDLRLQLDATLETERERLKGEAEGTKDDVRKARDEINRIQPGLMLPESSLDGFNFREL
                     DRKYGTGVRGGARLFGGGMGAEAVRELIVRMELDELAQKLHGEVRTTSGQRRKKAIKR
                     LRLIEAFRRSGNRPEWMIMSVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNR
                     LKRLLELGAPEIIIRNEKRMLQEACDALIDNGRRGRSIVGTGNHRLKSLSDMLKGKQG
                     RFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFVMRQLVEKALAHNI
                     KSAKRIVERASSRPEVWDVLEEVIKDHPVLLNRAPTLHRLGIQAFMPVLVEGSAIQIH
                     PLVCFAFNADFDGDQMAVHVPLSRAAQEEARRMMLSTANLLSPSNGEPVVGPTQDMVL
                     GCYYLTLEEEPTQGKAALSRFSDEQEVLLAYDVGKIDTRTDVGLKNHSVPKLSLHSPI
                     EVTVKGWDEAAGEITDQVVRTTVGRVIFNQALPDRLRFANRTMNRTALREIVAECYRL
                     LGPNETAHLVDGIKSVGFHYATRGGMTIAVDDIAVPPAKGSLLKAADEQVEAIDKQFQ
                     RGLITDDERYEQVVDVWKKTTQTISDQMMEALDPTGAVTMMTASGARGNKGNIGQLGG
                     MRGLMADPTGRIIDVPVRSNFREGMTVLEYFISTHGARKGLADTALRTADSGYLTRRL
                     VDVAQDVITRADDCGTEEGTWITRGETEEYAPEADAFQRRVVGRYAAGPVADPRVPKK
                     GAEPIVARNQEITEDIANRIVEAGVEEVLVRSPLTCQLHHGVCRLCYGRNLATGNLVG
                     IGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGLDITAGLPRVEELFEARMPKGKAEIS
                     HIDGVVEVIQTEGGARRVKVTSREEFDTPLRIAKGLEVLVAEGDHVEANQIVARGPET
                     KKKDAKADELRAPEKGLMVKTADGLRIRSEDVVEREYAIPHNAKLLVENGQMVRAGDP
                     ITDGPINPQEYLETRGRDAVQRYLVKEVQRVYRSQGVTINDKHIEIIVRQMLRKVRID
                     QPGDSEQLPFELVDKFDFEELNNQVMAEGGEPATAETVLLGVTKASLNTASFLAAASF
                     QETTRVLTEAAITGSKDKLLGLKENVIIGKLIPAGSGAPQNLAAARAAARRAAAEALA
                     GGELPEGFGDEEINIFLAEAQARAEAEAEAAARQDFNPLLGDAADGDGALDGQNPFLM
                     EDEEEDDEDDELPALEEEAEAPAATATATKAKAPAKTKAAAKTKS"
     gene            4601..5014
                     /gene="rpsL"
                     /locus_tag="VIF08_01555"
     CDS             4601..5014
                     /gene="rpsL"
                     /locus_tag="VIF08_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY8134695.1"
                     /translation="MPTISQLVRHGRQPRIRKVKAPAMRKNWNSLHQKVEPIPGAPQK
                     RGVCLQVRTMTPKKPNSALRKIARVRLSNQQEVTAYIPGIGHNLQEHSVVLVRGGRVR
                     DLPSVKYHIIRGTLDAAGVRDRKQSRSLYGAKKPK"
     gene            5025..>5327
                     /locus_tag="VIF08_01560"
     CDS             5025..>5327
                     /locus_tag="VIF08_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012404.4"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY8134696.1"
                     /translation="MPRRASIARKEKYAPTLSRTTDKIVVKLMMNGKKGLAERILRQA
                     LARAEASAKRPGLEVLDAALRNATPIIEVKPRRVGGATYQVPVEIRGDRRMSLGVRW"
BASE COUNT          996 a         1806 c         1782 g          743 t
ORIGIN      
        1 gacgagctga aggagggcct caagaccctc aaggccggcg cggaggagga gcgcaccgcg
       61 cgcaccgccg agctggtcga gaagcagcag gccagcgagg ccaccatgaa ggccctgggc
      121 aaggcggcgg ccgaggagcc gatcgtcttc gagcccaccg gcgaggtcat cgttgccgcc
      181 ggcgacaagg ccgacaagcc ggctttcacg cgtctcaaca aggccgctgc ggcagaggtc
      241 gagcgcgtca acgacgagat caagggccgc gagaagaacg aagacgactc ggtcaagcgc
      301 cagatcgagg acctgcgcct ccagctggac gcgacgctcg agacggagcg cgagcgcctc
      361 aagggtgagg ctgagggcac caaggacgat gtccgcaagg cgcgcgacga gatcaaccgc
      421 atccagcccg ggctgatgct gcccgagtcg agcctcgacg gctttaactt ccgcgagcta
      481 gaccgcaagt acgggaccgg tgtgcgcggt ggcgctcgcc tgttcggcgg cggcatgggc
      541 gcggaagcgg tgcgcgagct gatcgtgcgc atggagctcg acgagctcgc ccagaagctg
      601 cacggcgagg tccgcaccac gagcggccag cggcgcaaga aggcgatcaa gcggctgcgc
      661 ctgatcgagg ccttccgccg ctcgggcaac cggccggagt ggatgatcat gtccgtcctg
      721 ccggtcatcc cgcccgacct gcggcccatg gtccagctcg acggtggccg cttcgccacg
      781 tccgacctga acgacctgta tcggcgcgtc atcaaccgca acaaccgcct caagaggctg
      841 ctcgagctgg gcgcgcccga gatcatcatc cgcaacgaga agcgcatgct ccaggaggcc
      901 tgtgacgcgc tgatcgacaa cggccgccgc ggccgctcga tcgtcggcac cggcaaccac
      961 cgcctgaaga gcctgtcgga catgctcaag ggcaagcagg gccgcttccg ccagaacctg
     1021 ctcggcaagc gcgtcgacta ctcgggccgt tcggtcatcg tggtcggccc ggagctgaag
     1081 ctgcaccagt gcggcctgcc caagaagatg gcgctcgagc tgttcaagcc cttcgtcatg
     1141 cgccagctcg tcgagaaggc gctcgcccac aacatcaaga gcgccaagcg catcgtcgag
     1201 cgggcgtcga gccggcccga ggtctgggat gtccttgaag aggtcatcaa ggaccacccg
     1261 gtgctcctca accgcgcacc cacgctccac cgcctgggca tccaggcgtt catgccggtg
     1321 ctcgtcgagg gctcggcgat ccagatccac ccactcgtct gcttcgcgtt caacgccgac
     1381 ttcgacggcg accagatggc cgtccacgtt cctctctcgc gcgcagcgca ggaagaggcg
     1441 cggcggatga tgctctcgac cgccaacctg ctgtcgccgt cgaacggcga gccggtcgtc
     1501 ggtcccacgc aggacatggt cctgggctgc tactacctga ccctcgaaga ggagcccacc
     1561 cagggcaagg ccgcgctctc gcggttctcc gacgagcagg aggtcctgct cgcctacgac
     1621 gtgggcaaga tcgacacccg cacggacgtc ggcctgaaga accactcggt gcccaagctc
     1681 agcctgcatt cgccaatcga ggtcacggtc aagggctggg acgaggccgc tggcgagatc
     1741 accgatcagg tggtccggac gacggtcggc cgggtcatct tcaaccaggc gctgcccgat
     1801 cggctgcgct tcgccaaccg gaccatgaac cgcaccgcgc tgcgcgagat cgtcgcggag
     1861 tgctaccgcc tgctcgggcc taacgagacg gcgcacctgg tcgacggcat caaatcggtc
     1921 ggcttccact acgcgacgcg cggtgggatg acgatcgccg tcgatgacat cgccgtgccg
     1981 ccggccaagg gcagtctgct gaaggcagcc gacgagcagg tcgaggcgat cgacaagcag
     2041 ttccagcgcg gtctcatcac cgacgacgag cgctatgagc aggtcgtcga tgtgtggaag
     2101 aagaccactc agaccatcag cgaccagatg atggaggcgc tcgatccgac gggcgccgtg
     2161 acgatgatga ccgccagtgg cgcccgcggt aacaagggca acatcggcca gctgggcggc
     2221 atgcgcggcc tgatggccga ccccaccggt cggatcatcg acgtgcccgt gcggagcaac
     2281 ttccgcgagg gcatgaccgt gctcgagtac ttcatctcga cccacggcgc gcgcaagggt
     2341 ctggctgata cggctctacg aacggcggac tcggggtatc tgacccgccg gctcgttgac
     2401 gtcgcccagg acgtcatcac ccgtgccgac gattgcggca cggaggaagg cacctggatc
     2461 acacgcggcg agaccgagga gtacgcgccc gaggcggacg cgttccagcg ccgtgtcgtg
     2521 ggccgctatg ccgcgggacc tgtcgcggat ccgcgcgtgc ccaagaaggg cgccgagccc
     2581 atcgttgcgc gcaaccagga gatcacggaa gacatcgcca accgcatcgt cgaggctggc
     2641 gtcgaggaag tgctggttcg atcaccgctg acgtgccagc tgcaccacgg agtctgccgc
     2701 ctgtgctatg gccgcaacct tgccaccggg aatctggtgg gcatcgggga ggctgtcggg
     2761 atcatcgctg ctcagtcgat cggtgagccc ggcacgcagc tgacgatgcg gacgttccac
     2821 accggcggcg tcgccggcct ggacatcacc gccggcctgc cgcgcgtcga ggagctgttc
     2881 gaggcgcgca tgcccaaggg caaggccgag ataagccaca tcgatggcgt ggtcgaggtc
     2941 atccagaccg agggtggcgc gcggcgcgtc aaggtcacct cgcgcgagga gttcgacaca
     3001 ccgctgcgca tcgccaaggg tctcgaggtg cttgtcgcag agggcgacca tgtcgaggcc
     3061 aaccagatcg tcgcccgcgg gccggaaacc aagaagaagg acgccaaggc cgacgagctc
     3121 agggcgcccg agaagggcct catggtcaag accgcggacg gccttcgaat tcgctcggag
     3181 gacgtggtcg agcgcgagta cgccatcccg cacaacgcca agctgctcgt tgagaacggg
     3241 cagatggtgc gcgccggtga cccgatcacc gacgggccga tcaacccgca ggagtacctc
     3301 gagacgcgcg gtcgcgatgc cgtgcagcgc tacctggtca aggaagtgca gcgcgtgtat
     3361 cggtcacagg gcgtgaccat caacgacaag cacatcgaga tcatcgtccg ccagatgctg
     3421 cgcaaggtgc gcatcgatca gcccggtgat tcggagcagc tgccgttcga gctggtcgac
     3481 aagttcgact tcgaggagct caataaccag gtcatggccg agggcggcga gccggcaacg
     3541 gctgagaccg tgctgctcgg cgtgaccaag gcgtccttga acaccgcttc attccttgcc
     3601 gccgcctcct tccaggagac gacgcgcgtc ctgacggagg cggccatcac cggctccaag
     3661 gacaagctgc tggggctcaa ggagaacgtc atcatcggca agctcatccc ggccgggtcg
     3721 ggcgcgccac agaacctcgc tgccgctcgg gctgccgccc ggcgcgccgc agccgaggcg
     3781 ctggccggtg gcgagctgcc ggagggcttc ggtgacgagg agatcaacat cttcctggca
     3841 gaggcgcagg cccgcgccga ggcagaggcg gaagccgctg cccggcagga cttcaacccg
     3901 ctgctgggtg acgcagccga cggcgatggc gccctcgacg gccagaaccc gttcctgatg
     3961 gaggacgagg aagaggacga cgaggacgac gagctgccgg cgctcgagga agaggctgag
     4021 gcgccggccg cgacggcgac cgcgaccaag gccaaggcac ctgccaagac aaaggcggcc
     4081 gccaagacca agagctagac tgctcttaca acctcgattt tcagagaacc cgccggacta
     4141 tcacccggcg ggttttcgta tgtccgcgcg ggagctgcgg cgggtcctgt ccttgcgacc
     4201 gtggtcacgc ctcggccctg gccctccggg cctcttacgg gccttcgggt gacgcagcgg
     4261 tcgccggcca cccgcctccc tcgccccacg cccaacgtcc cttgcatcgt cgcgtccctt
     4321 gggccacgcc tcaagtagag ccgtcaccgc gctcgattag cattgcagga gacgacgtgc
     4381 cgttgccatg aatggcaggt ttttggtaca ctccgcgatc gtggcctgag ggccgtatgt
     4441 cgttcccgat ggggagcggc accgaggcaa taaccgccca ggtgctgagc atcgggtcca
     4501 accccgccag ttgaaatagt ccgtccggct cgcgcgcgcg aattgcgcgg gggagggccg
     4561 gcgagtgcag cagtcaccaa agacaaggga gtcgtacttc gtgccgacga tcagccagct
     4621 cgtgcgccac ggccgccagc ctcgcatccg caaggtcaag gcgcccgcga tgcgcaagaa
     4681 ctggaacagc cttcaccaga aggtcgagcc gatcccgggc gccccgcaga agcgcggcgt
     4741 ctgtctccag gtgcgcacga tgacccccaa gaagcccaac tcggcgctgc gcaagatcgc
     4801 ccgcgtccgc ctgtccaatc agcaggaagt gaccgcctac atcccgggca tcggccacaa
     4861 cctgcaggag cactcagtgg tgctcgtgcg cggtggccgc gtgcgcgacc tcccgtcagt
     4921 caagtaccac atcatccgcg gcacccttga tgccgccggc gtgcgcgacc gtaagcagag
     4981 tcgctcgctg tacggcgcca agaagcccaa gtaggtagcg agcgatgccc cgtcgagcct
     5041 ccatcgcgcg caaggaaaag tacgcgccga cgctgtcccg cacgactgac aagatcgtcg
     5101 tcaagctgat gatgaacggc aagaagggtc tcgccgagcg catcctgcgc caggcgctcg
     5161 cccgcgctga ggcgtcagcc aagcgacccg gcctcgaggt gctcgacgcc gcgctgcgca
     5221 acgccacccc aatcatcgaa gtcaagccgc gccgcgtcgg tggcgccact taccaggttc
     5281 cggtcgagat ccgcggtgat cgccgcatga gcctcggcgt gcgctgg
//
LOCUS       DATBRX010000045        10025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2360826, whole genome shotgun sequence.
ACCESSION   DATBRX010000045 DATBRX010000000
VERSION     DATBRX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360826"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VIF08_01565"
     CDS             complement(<1..595)
                     /locus_tag="VIF08_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134697.1"
                     /translation="MRNFWHSLRRSVEQRPRATLAAGLIAVALVAAGVTWIVSNPART
                     PPVDVPTATATERWTLLELATRIDAGQVTAVTVGAGESGGRVLLAQRADGAYVAITFN
                     GSVGDAAAALQTLGYDDLLTPSAHAAIDASAASPSAGDPLRNAISVGLVVLLAGVAVS
                     FIYSARDRLPVLGRRGSRFSAIMPATPRSAASGLPVPG"
     gene            complement(624..1181)
                     /locus_tag="VIF08_01570"
     CDS             complement(624..1181)
                     /locus_tag="VIF08_01570"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8134698.1"
                     /translation="MSDEVLISVDIEASGDSPSNGSLLSIGACLVDDPSVGIYLELTP
                     VPDMPWSAEAEGVHKLTREHLERNGLDPAEAMRRLDGWVRDTAAGRWPVFVGFNAPFD
                     WMFVADAFWRYLARNPFGISALDLKSLYMGSHGVSSWAQTRKVHVERRLGVHFEHTHN
                     ALADARDQARLAQVLLLHLRRKEPE"
     gene            complement(1609..2112)
                     /locus_tag="VIF08_01575"
     CDS             complement(1609..2112)
                     /locus_tag="VIF08_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8134699.1"
                     /translation="MICPSCGFENLQGTDECDNCGADLRIADLPQPSSGFEKDLVSMP
                     LAAVKHHTPLTIAPTASAADAVAQMQAANVGCLLVEDASGALAGVLSERDLLLRLTGT
                     RLEGVTVADLMTRDPVVLRATDSVAVAIHKMALGGFRHIPLVADGRATGIVSARDIFA
                     HILAALR"
     gene            complement(2201..3289)
                     /locus_tag="VIF08_01580"
     CDS             complement(2201..3289)
                     /locus_tag="VIF08_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY8134700.1"
                     /translation="MTDGNGKGTTTNGNGAKLSPENAQVLETLQLTRKDFVSDQEVRW
                     CPGCGDYSILAQTQKTMPDFGVPRENIVFISGIGCSGRLPYYMNTYGFHSIHGRAPTL
                     ATGLKLANPALMVWVITGDGDALSIGGNHILHSMRRNVDIKVIMFNNRIYGLTKGQAS
                     PTSELGKKTKSSPVGTVDYPIQPLSIALAAEASFVARSVDTHTEHLQETLDRAGHHHG
                     SAFVEVLQNCNIFNDGAYRDFTERDVRDDRMLALEHGKPMIFGKDRDKGIRLNGLHPE
                     VVTIGQNGVTEADLLVHDEEAQDPYLAYMLSRMWYPDFPVPVGVLRRVNRPTHDQLVV
                     GQIEDAITRHGAGDLDKLVLAGETWTVE"
     gene            complement(3289..5214)
                     /locus_tag="VIF08_01585"
     CDS             complement(3289..5214)
                     /locus_tag="VIF08_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEY8134701.1"
                     /translation="MTTETQQTKDVEVQELERVTIRFAGDSGDGMQLTGSQFTRTAAV
                     FGNDISTLPDFPAEIRAPAGSLPGVSGFQISFSSAEIYTPGDAPDVLVAMNPAALRTN
                     IGELPPGGALIVNSDAFNANNLKKAGYDANPLLDGSLKQYNVFEVPISTLNTRALEGL
                     DMTSKQVDLTKNFFALGMMFWLYERSMEPTLRWIEDKFGAKPVIAEANMRALKAGYAF
                     GETTEMFHTTYRVAPAKLAPGTYRNITGNEATALGFVVAAQKAGRELVYGSYPITPAS
                     DILHQLATYKNFGVKTFQAEDEIAAIGSAIGASYGGALGMTASSGPGIALKSEAMNLA
                     VMVELPLIVVDVQRAGPSTGMPTKTEQTDLLMAMFGRNGESPVPILAPATPAECFDYA
                     IEAVRLALKYMTPVMYLSDGFLANGSEPWRIPNLADLGELAVANHTDKATFQPYNRDP
                     DTLARPWAVPGTPGLEHRIGGLEKADITGNVSYDPENHHKMTLLRAAKIAGIADDIPP
                     LTVFGPEQGDVLVVGWGSTYGAIRSAVERLQAEGWAVSHAHLRHLNPLPENTEQVLRS
                     FKHVLVPEVNLGQLSLLLRAKFLIDVRGLNKVRGKPYRIIEIRQAVEALLSGKLIEDM
                     TLPADHTPSPSAGGGLA"
     gene            complement(5211..5753)
                     /locus_tag="VIF08_01590"
     CDS             complement(5211..5753)
                     /locus_tag="VIF08_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8134702.1"
                     /translation="MTFPGGPFRRVDGTAQTKSIGRTSAGMSRVDLRNENVRAVAQHE
                     PVSVTSDTPLDEAIAQMRGSGGDALLIIDGARLTGIVTERDVLTRVLGREIDGTRPVS
                     DFMSTEPRTLGADATLVEAMIAMESGHYRNLPLVDESGTVVGMLRQQDLLEYVAEAFP
                     QEILNLPPRPHQTMEAPEGA"
     gene            5951..6151
                     /locus_tag="VIF08_01595"
     CDS             5951..6151
                     /locus_tag="VIF08_01595"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY8134703.1"
                     /translation="MGNEQQNEGRMEQARGTIKETVGDLTDNERMQREGEYDKAKGNI
                     REGVGDVREDVDRTVDDLQDNR"
     gene            complement(6358..7089)
                     /locus_tag="VIF08_01600"
     CDS             complement(6358..7089)
                     /locus_tag="VIF08_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEY8134704.1"
                     /translation="MLGTGLLPYVLLVMLPGLGLTLWAAWRVRSTYARWSRMDSGISM
                     NPYDFARYLLNSQGLTDVSIEAVPGQLTDHYDPRGKVLRVSSAVAAQMPGQAPGYADA
                     RPHLSVAAVAVIAHEVGHAAQHRTGDPQMALRQTIVPVASLGSTIAPWLIIGGLIFRI
                     MDLAVVGLVFFAAAVVFTFVTLPVEFGASRRALAYVEPLGLTGERADGARSVLRAAGW
                     TYVAAALTALLTFLYYLSLIFGSRR"
     gene            complement(7137..7931)
                     /locus_tag="VIF08_01605"
     CDS             complement(7137..7931)
                     /locus_tag="VIF08_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134705.1"
                     /translation="MNATPDRTATRGLRARIAHYVRGVFEHVPASLGGHFQRLPTWLT
                     FGITGPFNGQRRRTAAVAAMFDAIPFGTVIETGTYRALTTRHLRTQTDAPIATIEVNP
                     AYYRYSQRRLAKLPRVDQFLGKSPAVLEQLRTDQTAWHAEPVFFYLDAHWLHDLPLLD
                     ELASIRRGWTEFAALIDDFAVDGDDGYFYDDYGPGKTLDLRHFAGHQELADLRVFWPA
                     APATSESGARRGWVVLASPGAVAEALSKLSELRRAGTLAEALAASR"
     gene            8037..8879
                     /locus_tag="VIF08_01610"
     CDS             8037..8879
                     /locus_tag="VIF08_01610"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEY8134706.1"
                     /translation="MFDSIGRSWELAKGSFRVLRSDKELLIFPLLSFIALVIVSISFF
                     FPFMWVGGVASAQNGEANIASYVVAFVFYVVSYTVTFFFQTALVGAAMIRLDGGDPTL
                     GDGLRIAFSRLPKIIVYAVIAATVGMILRWISERAGIVGQIVGGILGFAWSVATFLVV
                     PVLVVENVGPFEAIKRSTGYLKRTWGEQLIGNIGIGLVFGLLMMLVIFAGGALLFFLF
                     QASVTLGLIGVVALILLVGILALIGSALGGIFTASVYRYATKGDGGSMFNNQVLATAF
                     RQKH"
     gene            8897..>10025
                     /locus_tag="VIF08_01615"
     CDS             8897..>10025
                     /locus_tag="VIF08_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY8134707.1"
                     /translation="MNVVFLSPHFPPNWYRFAVALRASGATTLGIADVGFEQLQPELR
                     DALHDYYRVDDLADYHQLTRALGWFIHRHGRIDRIDSLNEHWLETEAALRSDFNIPGI
                     NMATISAIKRKSVMKQRFIDAGLKPGRGRVCHDEAELRDFIAEVGLPVVAKPDVGVGA
                     ARTYKIENEAEVEAYLADKPATDYIVEEFIRGELLTYDGMTGRDGEIVFDSTFQYSNG
                     VMEVVNAQLDIYYWMPRVIPADIQEVGRAMARAFDLRERPFHFEIFRTATGALIPLEV
                     NMRPPGGWTVDMWNYSNDFDFYREWANVVVNGVFGAEIARSYNVLYASRRDGRSYVLS
                     HDEVLSEFNELIVHESRMERVLASAMGDHGYVMRSPDLEPLV"
BASE COUNT         1631 a         3265 c         3423 g         1706 t
ORIGIN      
        1 tgcccgggac gggtaaacca ctcgcggccg agcgcggagt ggcaggcatg atggccgaga
       61 atctgctgcc ccggcgaccg agcaccggca gccgatcacg ggcgctgtag atgaatgaga
      121 ccgcgacccc ggcgagcagc accaccaggc ccacgctgat cgcgttgcgc aacgggtcgc
      181 ctgcggacgg gctcgcggcg ctggcgtcga tcgccgcatg cgcggacggt gtcagaaggt
      241 cgtcgtagcc gagcgtctgg agcgcggccg cggcgtcacc gaccgagccg ttgaacgtga
      301 tcgcgacgta tgcgccgtcg gcgcgctgcg ccaacaggac gcggccgccc gactcaccgg
      361 ctcccacagt gactgccgtg acctggccgg cgtcgatgcg cgtcgccagc tcgagcagcg
      421 tccagcgctc ggtcgcggtg gcagtcggga catcgacggg cggggtccga gccgggttgg
      481 agacgatcca ggtgacgccg gcagcgacca gcgcgactgc gatcaggccc gccgcgagcg
      541 tggcgcgcgg acgctgctca acgctgcgtc gtagcgaatg ccagaagttg cgcaactcgt
      601 ccccccgcga tgtcgctgca atgttactcc ggctccttgc gacgtagatg taacaaaagt
      661 acctgcgcca gccttgcctg atcgcgcgcg tctgccaggg cattgtgcgt gtgctcgaag
      721 tgcaccccaa gccgccgctc tacgtggact ttgcgcgtct gcgcccatga gctcacgccg
      781 tgactgccca tgtacaggct cttcaggtcc agcgccgata tcccgaacgg gttgcgggcc
      841 aggtagcgcc agaaggcgtc ggcgacgaac atccagtcga agggcgcgtt gaaccccacg
      901 aacaccggcc agcgacccgc ggccgtgtcg cgcacccagc cgtccaggcg acgcatcgcc
      961 tccgccgggt ccaagccgtt tcgctccagg tgttcgcgcg tgagcttatg cacgccctcc
     1021 gcctcggccg accagggcat gtcggggaca ggtgtgagct cgaggtagat gccgaccgat
     1081 gggtcgtcga cgaggcaggc cccgattgac agcaggctgc cattgctggg gctgtcaccc
     1141 gacgcctcga tatccacgct gatcaggact tcgtcgctca tcggcgcaaa tagtcgcacc
     1201 tgtgtgctcc acaccggaat tgcttccagc tccgcttgtg tccacagggt tttccccagt
     1261 ccgccagggt tcgtggaaaa cattggcccg attccggcaa atcgtggatg acttctcttg
     1321 tgcctcctgc gatgcgttcg taatctctgg tcagcccgaa ggggccagac ggaaatcgga
     1381 ccggacagat cggggagcaa tcccaagagg tgcggacccc gacccaggag acgcggaccc
     1441 gggactcgct cccgcggacc agcgaagcag gctcgcaaga gacttgccgt cgaatgccga
     1501 gaggctcgtc ctctcacgca gcggactacc tggcaccgat ggtcacttcg ggtctttcct
     1561 tgcccgccag gcagcgcccc gcagggcgat ggcggagact gaagcgcgct acctcagcgc
     1621 agcgagaata tgcgcaaaga tgtcgcgcgc gctgacgatg cccgtcgccc gaccgtctgc
     1681 gaccagcggg atgtggcgaa agccgcccag cgccatcttg tggatcgcca ccgccactga
     1741 gtccgtagcg cgcaacacga ccgggtcgcg cgtcatgaga tcggccaccg tcacgccctc
     1801 gagcctggtg ccggtcaggc gcagcagcag gtcgcgctct gacaggacac cggccagcgc
     1861 gccagatgca tcctcgacga gcaggcagcc cacgttcgcg gcctgcatct gggccaccgc
     1921 gtccgcggcg ctggcggttg gggcgatggt cagcggcgtg tgatgcttga ccgccgcgag
     1981 gggcatgctg acgaggtcct tctcgaagcc gcttgacggc tggggcaggt ccgcgatgcg
     2041 caggtcggca ccgcagttat cgcactcgtc ggtgccctgg aggttctcga agccgcacga
     2101 cggacagatc accgcaagcg accggacggc accgacgccc cgtcggggcc ggacgactgt
     2161 cgcgcgcagc tcggccgaat gacgcagcgg agcgaaagca ttactcgacc gtccaggttt
     2221 cgccggccag gaccagcttg tcgagatcgc ccgcgccgtg gcgcgtgatg gcatcttcaa
     2281 tctggcccac gacgagctgg tcgtgcgtcg gccggttgac gcggcgtagc acaccgacgg
     2341 gcacggggaa gtcggggtac cacatgcgcg acagcatgta ggcgaggtag gggtcctgtg
     2401 cctcttcatc gtgcacgagc aggtctgctt ccgtgacgcc gttctggccg atcgtgacga
     2461 cttccggatg aaggccgttg agccggatcc ccttgtcgcg gtccttgccg aagatcatgg
     2521 gcttgccgtg ctccagcgcg agcatcctgt cgtcgcgcac gtcgcgctcg gtgaagtcgc
     2581 ggtacgcgcc gtcgttgaag atgttgcagt tctggaggac ctcgacgaac gccgagccgt
     2641 gatggtggcc ggcgcggtcg agagtctcct gcaggtgctc ggtatgggta tccaccgagc
     2701 gcgccacgaa tgacgcctca gccgccagcg cgatggacag cggctggatc gggtagtcca
     2761 cagtgccgac cggcgatgac ttggtcttct tgcccagctc agaggtcggc gacgcctggc
     2821 ccttggtcag gccgtagatg cggttgttga acatgatgac cttgatgtcg acgttgcgcc
     2881 gcatcgaatg gaggatgtgg ttgccaccga tcgatagcgc atctccgtcg ccggtgatga
     2941 cccacaccat cagcgcgggg ttggccagct tcaggccggt cgcgagcgta ggcgctcggc
     3001 cgtggatcga gtggaagccg taggtgttca tgtagtaggg cagccggccc gagcagccga
     3061 tgccgctgat gaagacgatg ttctcgcgcg gcacgccgaa gtcgggcatc gtcttctgcg
     3121 tctgagccag gatcgagtag tcgccgcagc ccgggcacca gcgcacttcc tggtcggaca
     3181 cgaagtcctt gcgcgtgagc tggagcgtct cgagcacctg ggcgttctcc gggctcagct
     3241 tggcgccgtt gccgttggtc gtcgtgccct tgccattgcc gtcggtcatc aggccagtcc
     3301 tccgcctgcg cttgggctgg gcgtgtggtc ggccgggagc gtcatgtcct cgatcagctt
     3361 gccgctcagc aatgcctcga cggcctgcct tatctcgatg attcggtacg gcttgccgcg
     3421 taccttgttg agcccccgca cgtcgatcag gaacttcgcc cgcaggagca gcgacagctg
     3481 gcccaggttg acctcgggca cgagcacgtg cttgaagctg cgcagcactt gctctgtgtt
     3541 ctcgggcagc gggttcaggt ggcgcagatg ggcgtggctg accgcccagc cctccgcctg
     3601 caagcgctcg acggcactcc tgatggcgcc gtaggtcgag ccccagccga ccaccagcac
     3661 gtcgccctgc tccggcccga agaccgtcag cggcgggatg tcatcggcga tgccggcgat
     3721 cttggccgcg cgcagcaacg tcatcttgtg atggttctcc gggtcgtatg agacgttgcc
     3781 ggtgatatcg gccttttcca gaccgccgat gcgatgctcg aggccggggg tgccgggcac
     3841 agcccacggc cgggcgagcg tgtcgggatc acggttgtac ggctggaacg tcgccttatc
     3901 cgtgtggttc gccacggcga gctcaccgag gtcggccagg ttcgggatgc gccacggctc
     3961 cgacccgttg gccaggaagc cgtccgacag gtacatgacc ggggtcatgt acttgagcgc
     4021 caggcgcacg gcctcgatgg cgtagtcgaa gcactcggcc ggcgtcgccg gggcgaggat
     4081 gggcacgggc gattcgccat tgcgcccgaa catcgccatg agaaggtcgg tctgctcggt
     4141 cttggtcggc atgcccgtcg atggtccggc acgctgcacg tcgaccacga tgagcggcag
     4201 ctcgaccatc acggcgaggt tcatggcctc gctcttgagc gcaatgccgg gacccgagct
     4261 ggcagtcatc cccagcgcgc caccgtacga ggcgccgatc gccgatccga tcgcggcgat
     4321 ctcatcttcg gcctgaaatg tcttgacccc gaagttcttg tatgtcgcga gctggtgcag
     4381 gatgtcgctc gccggcgtga tggggtaaga gccgtagacc agctcgcgcc ccgccttctg
     4441 ggcggccacg acgaagccga gtgccgtcgc ctcattgccg gtgatgttgc ggtacgtgcc
     4501 cggcgcgagc ttggccggcg ccacgcgata ggtcgtgtgg aacatctcgg tcgtctcgcc
     4561 gaacgcatag cctgccttga gggcacgcat gttcgcctcg gcgatgaccg gcttggcgcc
     4621 gaacttgtct tcgatccagc gcagcgtcgg ctccattgac cgctcgtaca gccagaacat
     4681 catgcccagc gcgaagaagt tcttcgtcag gtcgacctgc ttggaggtca tgtccaggcc
     4741 ctcgagggcg cgcgtgttga gcgtgctgat cgggacctcg aagacgttgt attgcttgag
     4801 cgagccgtcc aggagcggat tggcgtcgta gcccgccttc ttgaggttgt tcgcgttgaa
     4861 tgcgtcgctg ttgacgatga gcgcgccacc cggtggcagc tcgccgatgt tcgtccggag
     4921 cgcggccggg ttcatcgcca cgagcacgtc gggcgcgtca ccgggggtat agatctcggc
     4981 actcgagaat gagatctgga agccggagac gccgggcagc gagccggcgg gcgcgcggat
     5041 ctccgcgggg aagtcgggca gggtgctgat gtcgttgccg aagaccgcgg cggtgcgcgt
     5101 gaactgtgag ccggtcagct gcatgccgtc gcccgagtca ccggcgaagc ggatggtgac
     5161 tcgctcgagc tcctggacct cgacgtcctt ggtctgttgg gtctctgttg tcacgccccc
     5221 tcgggtgcct ccatggtctg gtgcggtctt ggcggtaggt taaggatttc ctgggggaag
     5281 gcctcggcca cgtattcgag caggtcctgc tggcggagca tgccgacgac cgtgccgctc
     5341 tcgtcgacga gcggcaggtt gcgatagtgg ccgctctcca ttgcgatcat cgcctcgacc
     5401 agggttgcat cggcgcccag ggtgcgcggc tccgtggaca tgaagtcgct caccggccgg
     5461 gtgccgtcga tctctcggcc aaggacgcgg gtcagcacgt cgcgctccgt gacgatgccc
     5521 gtgagccggg cgccgtcgat gatgagcagc gcatcaccac cgctgccccg catctgggca
     5581 attgcctcgt cgagcggcgt gtcagacgtg acgctcaccg gctcgtgctg ggcgacagcg
     5641 cgcacattct cattgcgcag atcgacgcgg ctcatgccgg cgctcgtgcg gccgatgctc
     5701 ttggtctggg cggtgccgtc cacgcggcgg aatggacctc cgggaaatgt cacgctcggg
     5761 gccagggtat ttcgatgccc gaagacgccc gcagattcta tctcgcgtgc ggcccaatcg
     5821 aaattagatg gatgaaaggc cccccaccgc ggcccgttga ggtgaatgac gcgcgccaca
     5881 ctggaccatg gctcagcaat cggcccaacc gtgtcagtgg tcgatgccct gaacaaggga
     5941 ggagtcacag atgggcaacg agcagcagaa cgagggccgc atggagcagg cccgcggcac
     6001 gatcaaggag accgtcggcg atctgaccga caacgagcgc atgcagcgcg aaggcgagta
     6061 cgacaaggcg aagggcaaca tccgcgaggg cgtgggtgac gttcgcgagg atgtcgatcg
     6121 caccgtcgac gatctgcagg acaaccgctg acgaccaaac cggcggtcaa gtccgccgac
     6181 caagtgatca cctccgcaga cggcctgggg gccgctgctg cgatctccgc gcaagcccgc
     6241 gcatgcgcgt gcgtgcgctg cgcttgcgaa tgacttagct cggccgtgtt atcgaccgag
     6301 tgaaaccggg gggaccgggt cgccgagagg cggcccggct ctctcttttc tgggggccta
     6361 gcggcgcgag ccgaagatca gcgacaggta gtacaggaac gtgagcagcg cggttagggc
     6421 ggcggcgacg taggtccagc cggcggcacg cagcactgag cgggcgccgt cagcgcgctc
     6481 accggtcagg ccaagcggct cgacgtaggc aagggcgcgg cggctcgcgc cgaactcgac
     6541 gggcagcgtc acgaaggtga agacgacggc cgcggcgaag aacacgaggc cgaccaccgc
     6601 gagatccatg atccggaaga tgagcccgcc gatgatcagc cacggcgcta tggtcgaacc
     6661 gaggctcgcg acgggcacga tcgtctggcg cagcgccatc tgcggatcgc cggtgcggtg
     6721 ttgtgccgcg tggccaacct cgtgggcaat gacagcgacc gccgcgaccg ataggtgcgg
     6781 tcgcgcgtcg gcgtagcccg gcgcctggcc gggcatctgc gccgcgaccg cggagctgac
     6841 gcgcagcacc ttgccccgcg ggtcgtagtg gtccgtcagc tgccccggaa cggcctcgat
     6901 gctgacgtcg gtcaggccct gcgagttgag cagatagcgc gcgaagtcgt agggattcat
     6961 tgagatgccc gagtccatgc ggctccagcg cgcataggtc gaacggacac gccacgctgc
     7021 ccacagggtc aaacccaggc cgggcagcat caccaggaga acgtaaggca ggagaccggt
     7081 gcctagcatc ggttcacgtt accccaaaac ggcttggcgg cccctccgcg cgcgggtcag
     7141 cgcgaggccg cgagggcctc cgccagcgtg cccgcgcggc gcagctcgga gagcttgctc
     7201 agcgcctccg cgaccgcgcc cggcgacgcg agcacgaccc agccgcgccg cgcgccggac
     7261 tcgctggtcg cgggcgccgc cggccagaag acgcgcaggt cggccagctc ctggtgcccc
     7321 gcgaagtgac gcaggtcgag cgtcttgccg gggccgtagt cgtcgtagaa atacccgtcg
     7381 tcgccatcga ccgcgaagtc atcaatgagc gccgcgaact ccgtccagcc acggcgtatc
     7441 gacgcgagct catcgaggag cggcaggtcg tgcagccaat gcgcgtcgag gtagaagaag
     7501 acgggctcgg cgtgccacgc cgtctgatcg gtgcgcagct gctccagcac cgcgggcgac
     7561 ttgcccagga actggtcgac gcgcggcagc ttcgcgaggc ggcgctggga gtagcggtag
     7621 taggccgggt tgacctcgat ggtggcaatc ggcgcatcgg tctgcgtcct caggtggcgc
     7681 gtcgtcagcg cccggtacgt gccggtctcg atgaccgtgc caaaaggaat cgcgtcgaac
     7741 atcgccgcaa ccgctgccgt gcgccggcgc tggccgttga acggccccgt gatgccgaac
     7801 gtcagccacg ttggcagccg ctggaagtgg ccgccgagcg atgccgggac gtgctcgaag
     7861 accccgcgta cgtagtgggc gatccgggca cgcagcccgc gggtggcagt ccggtcgggc
     7921 gtagcgttca cggcgccggg atactaccca ctctggcgcg gagagggatg agcggctagg
     7981 ctcattggac cggcgtgtgc aggggcgtcg ggcaagctga agggaaggcg tcaaagatgt
     8041 tcgattcgat cggccgcagc tgggagctgg ccaagggcag cttccgggtc cttcggtccg
     8101 acaaggagct gctcatcttc ccgctccttt cgttcatcgc cctggtcatc gtctcaatca
     8161 gcttcttttt cccgttcatg tgggtgggcg gcgttgcgag cgcccagaac ggcgaggcga
     8221 acatcgcgtc atacgtggtc gcgttcgtct tctacgtcgt ttcgtacacg gtgacgttct
     8281 tcttccagac cgcgctggtg ggcgcggcga tgattcgcct ggacggcggc gaccccacgc
     8341 tgggcgacgg gctgcgcatt gcgttctcgc gcctgcccaa gatcatcgtc tacgccgtca
     8401 ttgcggccac ggtaggcatg atcctgcgct ggatctcaga acgcgccggg atagtcggcc
     8461 agatcgtcgg cggcatactc ggcttcgcct ggagcgtcgc caccttcctg gtcgtgcccg
     8521 tcctcgtggt cgagaacgtg ggcccctttg aagcgatcaa gcgcagcacc ggctacctga
     8581 agcgcacctg gggcgagcag ctcatcggca acatcggcat cggcctggtc tttggcctgc
     8641 tgatgatgct cgtcatcttt gccggcggag cgctgctgtt cttcctgttc caggcgtcgg
     8701 tcaccctggg cctgatcggc gtggtggcgt tgatcctgct ggtcggcatc ctggcgctca
     8761 ttggctcagc actgggcggc atcttcaccg cgagcgtcta tcgctacgca accaagggcg
     8821 acggcggcag catgttcaac aaccaggtcc tcgccacggc cttccgccag aagcattgag
     8881 tctggagtgg ggctgagtga acgtcgtctt cctctcgccg cactttccgc ctaactggta
     8941 tcgcttcgcg gtcgcgcttc gcgcttccgg cgccacgacg ctgggcatcg cggacgtcgg
     9001 tttcgagcag ctccagccgg agctgcgcga cgccctgcac gactactacc gtgtcgacga
     9061 cctggctgac taccaccagc tgacgcgcgc cctgggctgg ttcatccatc gccacggccg
     9121 cattgatcgc atcgactcgc tcaacgagca ctggctcgag acagaggccg cgctgcgcag
     9181 cgacttcaac atcccgggca tcaacatggc gacgatcagc gcgatcaagc gcaagtcggt
     9241 catgaagcag cgcttcatcg acgccggtct caaaccgggc cgcggccgcg tctgccacga
     9301 cgaggcggag ctgcgcgact tcatcgccga ggttggcctg ccggtggtgg ccaagcccga
     9361 cgtgggagtc ggtgccgcgc gtacgtacaa gatcgagaac gaggccgagg tggaggccta
     9421 cctggccgat aagcccgcca ccgactacat cgtcgaggag ttcatccgcg gcgagctgct
     9481 gacctacgac ggcatgaccg ggcgcgacgg cgagatcgtc ttcgactcga cgttccagta
     9541 ctcgaacggc gttatggaag tggtcaacgc ccagctcgac atctactact ggatgccgcg
     9601 cgtcatcccc gccgacatcc aggaggtggg tcgcgcgatg gcccgcgcgt tcgacctgcg
     9661 cgagcggccg ttccatttcg agatcttccg caccgcgacc ggcgctctca tcccgctcga
     9721 ggtcaacatg cgcccgcccg gcggctggac cgttgacatg tggaactact cgaatgattt
     9781 cgacttctat cgagagtggg ccaacgtcgt ggtcaacggc gtgttcggcg cagagatcgc
     9841 gcggtcgtac aacgtcctct acgcctcgcg ccgcgacggc cggtcctacg tgttgagtca
     9901 cgacgaggta ctcagcgaat tcaacgagct gatcgtccac gagtcgcgca tggagcgcgt
     9961 gctggcgagc gcgatgggcg atcacggcta cgtcatgcgc agcccggacc tggagccgct
    10021 cgtta
//
LOCUS       DATBRX010000046         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1913821, whole genome shotgun sequence.
ACCESSION   DATBRX010000046 DATBRX010000000
VERSION     DATBRX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913821"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..339)
                     /locus_tag="VIF08_01620"
     CDS             complement(61..339)
                     /locus_tag="VIF08_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134708.1"
                     /translation="MLEIAPAPVLSVLVGLFHTGIYLLIRGYAGFRLAFVGLAAILGA
                     YAGQALGSRLGDPLAIGDFGLLYASLLSWVGILIIVATSVLSSTRTSR"
     gene            complement(348..1430)
                     /gene="mnmA"
                     /locus_tag="VIF08_01625"
     CDS             complement(348..1430)
                     /gene="mnmA"
                     /locus_tag="VIF08_01625"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY8134709.1"
                     /translation="MLVAMSGGVDSSVAAALLAESGVDTVGVWMRLHDVADSYSEFKK
                     SCCSLDAADDARRVAGQLGIPFYVLNLEREFGVGVMQPFFDAYLDGRTPSPCVDCNSY
                     VKFGALLGKALRQFDCDAVATGHYARVDALEDADAPGGRRYRLLTGLDADKDQSYFLY
                     GLRQDQLAHTNFPLGSLTKPEVRDVARRHGLVTAEKPESQEICFVPGGDYRDALKTRA
                     GWTETPGNLVDSDGRTVGEHRGAAAYTIGQRSGLGVALGEPRYVSAIDAPSNVITLGR
                     REDLYRAEFSMERVSFVAGAPPRDDFSTHIRIRHRATPVPGSVSRRGEGWTVRLDTPT
                     WAPAPGQAAVFYNGDEVVGGGRIASA"
     gene            complement(1453..>2238)
                     /locus_tag="VIF08_01630"
     CDS             complement(1453..>2238)
                     /locus_tag="VIF08_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8134710.1"
                     /translation="NAINDRTILVSLMLANNEVGTIQPLADLVRRVRAHRGVAIHVDG
                     VQAAPYMAIDVQEMDVDLLSIASHKFEGPKGVGALYVRHGTILLPQMHGGSQERFRRA
                     GTENVAGAYGMAVAYEMARAEQLEVAPRLAALRDRLRDALTALPNVELTGHPRKRLPG
                     HLSVIARETDGEALILNLDMAGISASTGSACTTGSTEPSHVLTALGYPEEETRGSLRL
                     TLGRTTADADIDQAVVVVPQTISRLRSATAALVLDPLGEQVGA"
BASE COUNT          367 a          772 c          768 g          331 t
ORIGIN      
        1 cccgcggcat cgaccatgcg cttggctcgc tcgacggccg cggccgggcc gcgcacgatg
       61 tcaccggctc gtcctggtcg atgacaggac gctggtcgcg acgatgatca ggatgcccac
      121 ccagctcagc agcgaggcgt acagcagacc aaagtcaccg attgccagcg gatcacccag
      181 gcgcgacccc agcgcctgcc cggcgtaggc gcccaggatc gcggcgaggc ccacgaacgc
      241 gaggcggaag ccggcgtagc cacggatgag cagatagatc ccggtgtgga aaaggccaac
      301 cagcaccgat aggaccggtg cgggcgcgat ctcaagcatt ggctatctca cgcactggcg
      361 attcgtccac caccgacgac ctcgtcgccg ttgtagaaga ccgcagcctg ccccggggct
      421 ggtgcccagg taggcgtgtc gagtcggacg gtccagcctt caccgcgccg gctgaccgag
      481 cccggcacgg gagtggcccg atgacggatc cggatgtgcg tactgaaatc gtcgcgcggc
      541 ggcgcgccgg caacaaacga cacccgctcc attgagaact cggcgcggta caggtcctcg
      601 cgccggccga gcgtgatcac gttgcttggc gcatcgatcg cgctgacgta gcgcggctcg
      661 cccagggcaa cgcccagccc actgcgctgg ccgatcgtgt aggccgcggc tccgcggtgt
      721 tcgccaactg tgcgcccgtc ggagtcgacc aggttgcccg gagtctccgt ccagccggcg
      781 cgcgtcttta gcgcgtcgcg gtagtcgccg ccggggacga agcagatctc ctggctctcc
      841 ggcttctcgg cggtgaccag gccatggcga cgcgcgacgt cgcgcacctc gggcttagtc
      901 agggagccca acgggaaatt cgtgtgcgcc agctggtcct ggcgcaggcc atagaggaag
      961 tacgactggt ccttgtccgc gtcgagaccg gtcagcagcc ggtagcgtcg gccgcccgga
     1021 gcgtcggcat cctccaacgc gtcgacgcgg gcgtagtggc cggtcgcgac cgcatcgcag
     1081 tcgaactggc gcagcgcctt accgagcagc gcgccgaact tgacgtacga gttgcagtcg
     1141 acacacgggc tgggcgtgcg gccgtcgagg tacgcgtcaa agaacggctg catgaccccg
     1201 acgccgaact cgcgctcgag attcaggacg tagaacggga tgcccagctg cccggcgacg
     1261 cggcgcgcgt catcagcggc gtcgagcgag cagcaggact tcttgaactc gctgtacgag
     1321 tccgcgacgt cgtgcaggcg catccacacg ccgaccgtgt cgacgcccga ttcggccagg
     1381 agcgcggctg cgaccgatga atccacccca cccgacatcg cgacgagcac tcgtcgcttg
     1441 cgggcctcgc ggttaggcac cgacctgttc gccgagcggg tcgaggacga gcgcggcggt
     1501 cgcgctgcgc agcctgctga tcgtctgggg cacaacgacg acggcctgat cgatatcagc
     1561 gtcagcggtc gtgcggccca aagtcaggcg gagcgagccg cgcgtctcct cctccggata
     1621 gccgagcgcc gtaaggacgt gcgacggctc agtggagccc gtcgtgcacg ccgatccggt
     1681 cgatgccgag atgccagcca tgtccaggtt caggatcaac gcctcgccat ctgtctcgcg
     1741 ggcgataacg ctcaagtggc cgggcagtcg cttgcgtgga tggccggtca actcgacgtt
     1801 gggcagggcc gtgagcgcgt cgcgcaaccg gtcgcgcaac gcggccagac gcggcgcaac
     1861 ctcgagctgc tcggcgcgcg ccatctcgta tgccacggcc atcccgtacg cgcccgcgac
     1921 gttctcggta ccggcgcgac ggaagcgctc ctgcgagcca ccgtgcatct gcggcagcag
     1981 gatcgtgccg tggcgcacgt acagcgcgcc cacgcccttg gggccctcga acttgtgaga
     2041 cgcgatcgac agcaggtcca cgtccatctc ctggacatcg atcgccatgt agggcgcggc
     2101 ctgcacgccg tcgacgtgga ttgccactcc ccggtgggcg cgcacgcggc gcaccaggtc
     2161 ggcgagcggc tggatcgtgc cgacctcgtt gttggcgagc atcaggctca ccaggatcgt
     2221 gcggtcgttg atggcgtt
//
LOCUS       DATBRX010000047         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_350290, whole genome shotgun sequence.
ACCESSION   DATBRX010000047 DATBRX010000000
VERSION     DATBRX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350290"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VIF08_01635"
     CDS             <1..333
                     /locus_tag="VIF08_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134711.1"
                     /translation="TITAIAVALGESESRADQRALLAIGADPSLRRRIVAARAGVIAL
                     MAALLAIPAGLLPVWGLLASRDQPVVIPLPELLAIVVLLPLAAIAGALLFSRPIPAWS
                     AFREVSSG"
     gene            complement(330..1142)
                     /locus_tag="VIF08_01640"
     CDS             complement(330..1142)
                     /locus_tag="VIF08_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8134712.1"
                     /translation="MTSDPTERTGQKPRIKVEPQGDHPGVVLVTLDRPEVLNALDYQT
                     MGELTDALEKLDTDESVRCVVITGAGERAFAAGADIKEMAPATPVTMSVANNFARWER
                     LRRVRVPLIAAVRGFALGGGCELAMACDMVVASDDSQFGQPEIKLGIMPGAGGTQRLT
                     HALGKAKAMELILTGRNIGAAEALERGLVSQVVAREETLNTALALADEVASMPPLAVR
                     AAKESVNRADELSLEAGLEFERRNFFMLFASDDQKEGMAAFVDKRKPTWKGR"
     gene            complement(1139..>1966)
                     /locus_tag="VIF08_01645"
     CDS             complement(1139..>1966)
                     /locus_tag="VIF08_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY8134713.1"
                     /translation="ARRGTCLVTVEYSVKDRVATVRLNRPEVFNALNRETLESIRDSF
                     RKASADHSVGVVVLTGTGDKAFCTGADLDEQQAFLARPNDYWDWMGRFIEAIESIKDC
                     AKPVVARLNGMVVGGGNELNIACDLAVAADDVVLRHVGPSRGSLPAGGATQWMPLLVG
                     DRRAREVLLLNRPVPAATALEWGWINRVVPRTELDAAVADYCRELLDKMPEIVRATRV
                     QLNYWKSQAWAGTVQMAREWLTVHAASAEVAEGLASFHEKRPPDYEKLRTDIEEAGR"
BASE COUNT          294 a          696 c          633 g          343 t
ORIGIN      
        1 accattacgg caatcgcagt cgcgctgggg gagagtgagt cacgggccga tcaacgggcg
       61 ctactggcca taggtgccga tccttcacta cgtcgtcgaa tcgtggctgc cagggcgggc
      121 gtcatcgcac tgatggcggc acttctcgcg atcccagccg gtttgcttcc tgtctggggg
      181 ctgcttgcca gccgcgacca gcccgttgtc ataccgctgc ccgagctgct ggcgatcgtg
      241 gtcctcttgc cgctcgctgc aatcgctggc gcgctcctgt tcagtcgccc gattccagcc
      301 tggtccgcct tccgcgaggt cagttccggc tagcggccct tccaggtggg cttgcgcttg
      361 tcgacgaacg cggccatgcc ttccttctgg tcgtcggaag cgaagagcat gaagaagttg
      421 cggcgctcga attcgagccc cgcttccagg gatagctcgt cggcacggtt gactgattcc
      481 ttggccgccc tcacggcgag cggcggcatg gaggcgacct cgtcggcaag tgcaagggcg
      541 gtgttgagcg tctcttcgcg ggccacgacc tgcgagacga ggccgcgctc cagcgcctcc
      601 gcggcaccga tgttgcgccc cgtcaggatc agctccattg ccttcgcctt gcccagggcg
      661 tgcgtcaagc gctgcgtccc gcccgcgccg ggcatgatgc cgagcttgat ctccggctgg
      721 ccgaactggg agtcgtcaga cgccacgacc atgtcacacg ccatcgccag ctcgcagccg
      781 ccacccaggg cgaagccgcg caccgcggcg atcagcggga cgcgcacgcg acgcagccgc
      841 tcccagcgcg cgaagttgtt cgcgacggac atggtcacgg gagtcgcggg cgccatctcc
      901 ttgatgtccg ccccagcggc gaacgcccgc tcgcccgccc ccgtgatgac gacgcagcgc
      961 accgattcat cggtgtccag cttctccagt gcatcggtca gctcgcccat ggtctggtag
     1021 tcgagcgcgt tgagcacctc gggccgatcg agcgtcacca gcacgacgcc ggggtggtcg
     1081 ccctgtggtt cgaccttgat ccgtggcttc tggccggtgc gttcggtcgg atcggaagtc
     1141 atctgccggc ctcctcgatg tcggtccgca gcttctcgta gtcgggcggc cgcttctcgt
     1201 ggaacgacgc cagaccttcc gcgacctcag ccgaggcggc atgaacggtg agccactcgc
     1261 gcgccatctg cacggtcccc gcccacgcct gcgacttcca gtagttgagc tggacgcgcg
     1321 tcgcgcgaac gatctcgggc atcttgtcga gtagctcgcg gcagtaatcg gcgaccgcgg
     1381 catccaactc ggtccggggc acgacgcggt tgatccagcc ccactccaac gccgtggccg
     1441 cgggcacggg gcggttgagc agcagcacct cgcgggcgcg gcgatcccca actaacagcg
     1501 gcatccactg cgtcgcgccg cccgccggca acgagccgcg cgacgggccg acatggcgca
     1561 atacgacatc gtcggccgcg actgccaggt cgcacgcgat gttcagctcg ttgccgccgc
     1621 ccacgaccat gccgttgagc cgcgcgacga cgggcttggc gcagtccttg atcgattcga
     1681 tggcctcgat gaagcggccc atccaatccc agtagtcatt cggccgcgcg aggaaggcct
     1741 gctgctcgtc gaggtccgcg ccggtgcaga acgccttgtc gcccgtcccc gtgagtacga
     1801 cgacgcccac cgagtgatcg gccgaagcct tccggaatga atcccggatc gactccagcg
     1861 tttcccggtt cagcgcgttg aagacctccg gccggttgag ccggacggtc gcaacccggt
     1921 ccttgaccga gtactcaact gtcacgagac aggttcctcg tcgagc
//
LOCUS       DATBRX010000048         5778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_350365, whole genome shotgun sequence.
ACCESSION   DATBRX010000048 DATBRX010000000
VERSION     DATBRX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350365"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /gene="murB"
                     /locus_tag="VIF08_01650"
     CDS             <1..580
                     /gene="murB"
                     /locus_tag="VIF08_01650"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY8134714.1"
                     /translation="LEFGLAIPGTIGGAVWANAGAHESDVRAVIVEAGVLRADGEVTL
                     SREHLVLRYRDSVLKDVPFGAPDVVTWATFELEPADPAVVAGRLDEIRRWRQSHQPLG
                     MPSAGSVFRNPEGDSAGRIIDELGLKGARIGGATVSEKHANFIVNDERGTAADVRRLA
                     EQIQATVRRERGIELVFEIVFAGDWSGWEATA"
     gene            577..1704
                     /locus_tag="VIF08_01655"
     CDS             577..1704
                     /locus_tag="VIF08_01655"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY8134715.1"
                     /translation="MTDTGRVRVGVLAGGASSEREISLATGIEIASHLPADRYDVTLI
                     DPLALMAGNAALTDEQRAHARALAEHSGVIEELPERDRRELPAALQERIVKGSTGLVG
                     ANSALVPAGNEQRRLDVVFVAIHGQWGEDGTIQGMLELLGIPYVGSGVLASALAMDKV
                     MAKTVLAAHGIEVPAGIVVSRTGWPEERESTLARAEALGFPLVVKPVRQGSSVGVVMV
                     DATEQLLPAIEQGFALDDRVMVEERLLGTELTVGVIGNGPSLRALPVIEIATKAEFFD
                     YRAKYDPALTDEICPARISAELAARVQQMAMDAHNALGCRDMSRTDLIATAEGRVVAL
                     EVNTIPGMTANSLLPKAARVAGIEFGELCSMLIEAALARNS"
     gene            1725..2558
                     /locus_tag="VIF08_01660"
     CDS             1725..2558
                     /locus_tag="VIF08_01660"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEY8134716.1"
                     /translation="MRRPRAVDSGRAPSWLGVFNRARIAGLLLIVLSAGAVFSLVTDG
                     RFNLGASGATGDVEVSGLGYTDAAAVTEALALADTGGNNVFLLRTTDMRRRLLDLPSV
                     ADADVRVVLPHRLVVTITERTAVLRVAHRGATYLLDGEGVVLEQRPATAPPLPDLPLI
                     DDRRVELGIPFEVGVGIDPAEAQAMLQIGALTPALVGSAATELAFSADDTNGFVVTAG
                     PYGWRAVFGFYTPTLRPPTLIAQQVQCLRGLLASGEEQMDTIYLAPEGDRCGTYLPRP
                     S"
     gene            2574..3809
                     /gene="ftsA"
                     /locus_tag="VIF08_01665"
     CDS             2574..3809
                     /gene="ftsA"
                     /locus_tag="VIF08_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY8134717.1"
                     /translation="MEREAVLVALDVGTNKVVALIGEVTRDGAVTVIGKGAVPSSGIK
                     KGVVVNIDQTVSSIAAAREAAERLSGYRIESAIVGIGGNHVESMNSRGAVAVSGPRRE
                     VTREDIDRATEVARAVTIPSNREVLHVLPRGFVVDGQEGVKDPLGMSAVRLEVETHIV
                     HGSGTAIQNLTKCVGRAEIKIDELVAAALASADAVLTETERELGVAVADFGAGTLDLA
                     LYMEGSPFHTAVLPLGGNNVTNDIAIGLKTSLAAAEDLKVRVGTADPNGVEKDEQVDV
                     AGIGEGDASSASRYELALIIEARMREVFEKIGEQIEKAGSGGMLPAGLVLTGGAAQLA
                     GAARLGREVLQMPVRVAGPTGVSGLTDHLLTPAYSTSLGLLIWGAQTIHGDESRSYES
                     TPGPGMLGRMRDWARGLLP"
     gene            3959..4363
                     /locus_tag="VIF08_01670"
     CDS             3959..4363
                     /locus_tag="VIF08_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEY8134718.1"
                     /translation="MKRIEIAVQTGSRRGLFEITRECAAFVSGEGDGLLNVFVPHATC
                     GLVVMELGSGSDIDLMDALDRLLPRDDRWRHAHGSPGHGADHVLPLLVPPSITIPVVG
                     GELQLGTWQSIALLDPNPDNATRRVTLTFIAG"
     gene            4542..5750
                     /gene="ftsZ"
                     /locus_tag="VIF08_01675"
     CDS             4542..5750
                     /gene="ftsZ"
                     /locus_tag="VIF08_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8134719.1"
                     /translation="MPLRSDSENFALIRVIGIGGGGSNAVNRMIRAELMGVEFISVNT
                     DAQALLQSDAPHKIRIGDKITRGLGAGGDPGIGQRAAEEDAEKIFEALKDSDMVFITA
                     GMGGGTGSGAAPVIAEIARDLGALTIGVVTKPFGFEGARRRLNAEKGTDALRDKVDTL
                     ITIPNDRLRDVVSKETSILDAFRVVDDVLRQGVQGISDLITVPGLINLDFADVRTVMK
                     DAGSALMGIGRASGEGRAAQAARDAISSPLLEVNISGAQGVLFNVTGGSSLSLFEVDE
                     AAEIVKATADPEANIIFGTVIDERMGDEIAITVIATGFDNTRRREQTHAQVTEMAQVL
                     RSREREQQLDQRIAAAIPISTNGNGDSLPAREPAAATASSRTSGNDLRRGPQQEVEDL
                     DIPAFLRRRS"
BASE COUNT          914 a         1955 c         2019 g          890 t
ORIGIN      
        1 gctggagttc gggctggcga tcccggggac gatcggggga gcggtgtggg ccaacgccgg
       61 cgcgcatgaa tcggatgtcc gcgccgtcat cgtggaggct ggcgtgctgc gggcggatgg
      121 cgaggtgacc ctcagccgcg aacacctggt tctcaggtat cgcgacagcg tcctcaagga
      181 cgtgcccttc ggcgcgcccg atgtcgtgac ctgggcaacc ttcgagctgg agccggccga
      241 cccggcggtc gtcgccgggc ggctcgatga gatcaggcgc tggcgccagt cgcaccagcc
      301 gctgggaatg ccctcggcgg gcagcgtctt ccgcaacccc gagggtgatt cggcgggccg
      361 gatcatcgac gagcttggcc tgaagggcgc gcgcatcggt ggcgcgacag tgagcgagaa
      421 gcacgccaac ttcatcgtta acgacgaacg cggcacggcc gcggacgtgc gccgcctggc
      481 cgaacagatc caggcgacgg tgcgtcgcga acgcggcatc gagctcgtct tcgagatcgt
      541 gtttgccggt gactggtcgg gctgggaggc aaccgcgtga ccgatacagg gcgcgtcagg
      601 gtaggggtcc tcgccggtgg cgcgtcgagc gagcgcgaga tctcgctcgc cacgggcatc
      661 gagatcgcgt cccacctacc ggccgatcgc tacgacgtga cgctcatcga cccactcgcg
      721 ctgatggcgg gcaacgccgc gctgaccgac gagcagcgcg cccatgcgcg cgccctggcg
      781 gagcactccg gagtcatcga ggagctgccc gagcgtgatc ggcgcgagct gcccgccgcc
      841 ctccaggagc ggatagtcaa gggttccacc ggtctcgtcg gtgccaactc cgcgctcgtg
      901 ccggcgggca atgagcagcg ccggctcgac gtggtcttcg tcgcgatcca cggccagtgg
      961 ggcgaagacg ggacgatcca gggcatgctc gagctgctcg gcattcccta tgtggggtcg
     1021 ggcgttctcg cgtcggcgct ggcgatggac aaggtgatgg ccaagaccgt cctcgcggcg
     1081 catggcatag aagtgcctgc gggcatcgtg gtcagccgga cggggtggcc cgaggaacga
     1141 gagtcaaccc tcgcgcgggc ggaagctctc ggctttccac tcgtcgtcaa accggtgagg
     1201 caaggctcga gtgttggcgt cgtcatggtc gacgccacgg agcagctcct gcccgcaatc
     1261 gagcagggct tcgcactcga cgatcgcgtg atggtcgagg agcgcctgct tggcaccgag
     1321 ctgacggtcg gagtgatcgg caatgggccc agcctgcgcg cgctgcctgt catcgagatc
     1381 gcgaccaagg ccgagttctt cgactaccgc gccaagtacg acccggcgct gaccgacgag
     1441 atctgcccgg cgcgcatcag cgctgagctc gcggcgcgcg tccagcagat ggcgatggac
     1501 gcccacaacg cccttggctg tcgcgatatg tcgcggaccg acctcatcgc gaccgctgaa
     1561 ggccgcgtcg ttgccctgga ggtcaacacc atcccgggca tgaccgccaa cagcctgttg
     1621 cccaaggccg cccgtgtcgc aggtatcgag tttggcgagc tgtgcagcat gctcatcgag
     1681 gcggcgctcg cgcgcaattc atgacccgcc gcgccgcgcg gcccatgcgc cgaccacgcg
     1741 ccgttgacag cggccgcgcc ccatcctggc tgggcgtctt caaccgcgcc cggattgccg
     1801 gcttgctgct gatcgtgttg agcgccggcg ccgtcttttc gctcgtgacc gacggtcgct
     1861 tcaaccttgg ggcgtcgggc gccactggtg acgtcgaggt cagcggcctt ggctacaccg
     1921 acgcagccgc tgtgacagag gcgctggcgc tggctgacac tgggggcaac aacgtgttcc
     1981 tgctgcgcac cacagacatg cgccggcggc tgctcgacct gccgagcgtc gccgatgccg
     2041 acgtgcgggt tgtgctgccg catcggctgg tcgtcaccat caccgagcgg acggcggtcc
     2101 tgcgcgtggc ccaccgtggc gcgacgtacc tgttggatgg cgagggcgtg gtcctggagc
     2161 agcgccccgc aactgccccg ccgctgcccg acctgccgtt gatcgacgac cgccgggtgg
     2221 agctgggcat cccgttcgag gtcggcgtgg gcatcgatcc ggccgaagcg caggccatgc
     2281 tccagatcgg tgcgctcacg cctgcgttgg ttggctccgc ggcgacggag ctcgcgttca
     2341 gcgccgatga caccaacggt ttcgtggtaa cggccgggcc atatggctgg cgtgcggtct
     2401 ttggcttcta caccccgacg ctccgcccac ccacgctcat cgcccagcag gtccagtgcc
     2461 tgcgcggtct cctggccagc ggcgaggagc agatggacac gatctacctc gcgccggaag
     2521 gcgatcgctg cggcacgtat ctgccgcggc ccagttagga gagaatggcg cccgtggaac
     2581 gagaggccgt gctggtagcg cttgacgtgg gcacgaataa ggtcgtggcg ctcatcggcg
     2641 aggtgacgcg cgacggcgcg gtgaccgtta tcggcaaggg cgcggtccct tcgtcgggca
     2701 tcaagaaggg cgtagtcgtc aacatcgacc agaccgtctc gtcgatcgcc gcggcgcgcg
     2761 aggcggcgga gcggctgtcc ggctaccgca tcgaatcggc catcgtgggc atcggcggca
     2821 accacgtcga gtcgatgaac tcgcgtggcg cggtcgccgt gagcgggcca cgacgcgaag
     2881 tcacgcgcga ggacatcgat cgcgccacgg aggtggctcg cgcggtcacc atcccgtcga
     2941 atcgcgaggt gctgcatgtc ctgccgcgcg gcttcgtggt cgacggccag gagggcgtca
     3001 aggatccgct cggcatgagc gccgtgcggc tcgaggtgga gacccacatc gtccacggca
     3061 gcggcaccgc gatccagaac ctgaccaagt gcgtcggccg cgcggagatc aagatcgacg
     3121 agctcgtggc ggcagcgctc gcctcagcgg acgcggtcct gaccgaaacc gagcgtgagc
     3181 ttggcgtggc ggtcgccgac ttcggcgcgg gcacgctgga cctcgcgctg tacatggagg
     3241 gctcgccctt tcacacagcc gtgctgccgc tgggcggcaa caacgtgacg aacgacatcg
     3301 ccatcggcct gaagacatca ctcgccgcgg cggaggacct caaggtccgc gtcggcaccg
     3361 ccgacccgaa cggcgtcgag aaggacgagc aggtcgacgt ggcgggcatc ggtgagggcg
     3421 atgcgtcgag cgcctcgcgc tacgagctcg cgctgatcat cgaggcgcgt atgcgcgagg
     3481 tcttcgagaa gatcggcgag cagatcgaaa aggccggcag cggcgggatg ctaccggcgg
     3541 ggctcgtgct gaccggtggc gccgcgcagc tggcgggcgc cgcgcgcctt ggccgggaag
     3601 tgctccagat gccggtccgc gtcgccggcc cgaccggcgt atcgggcctg accgatcacc
     3661 tcctcacgcc ggcctactcg acgtcgctcg gcttgctcat ctggggtgcc cagacgatcc
     3721 acggcgacga gtcgcgctcg tacgagtcaa cacccggccc gggcatgttg ggccgcatgc
     3781 gcgactgggc ccgcggcctc cttccctgag cgccggggca aacctctccg tgcaggcgtg
     3841 atgcgcgcag cggctcaaac gtcggccttt ccctggccaa cgccacccga atcgccattc
     3901 tgcggcgact ttgctgcgcg aatcggcttt ggatgatgcg ccacgcgcat cacgcgcagt
     3961 gaagcggatc gagatcgccg tccaaacggg ttcgcggcgc ggcttgttcg agattacccg
     4021 cgagtgcgcc gcattcgtct ccggcgaagg cgacggcctg ctcaatgtgt tcgtgcctca
     4081 cgcaacatgc ggcttggtcg tcatggagct cggcagtggt tccgacatcg acctgatgga
     4141 cgcactcgac cggctgctgc cgcgcgacga tcgctggcgc cacgctcatg gctcgcccgg
     4201 ccacggcgcg gatcacgtcc tgccgctgct cgtgcccccg agcatcacca tcccggtcgt
     4261 cggcggcgag ctgcagctgg gcacctggca atcgatcgcg ctgctcgatc ccaacccgga
     4321 caacgccaca cgcagggtca cgctgacctt catcgcgggc tgaccagact ttccacaggc
     4381 cgtccacaca cggtccgaaa ttagatcttg ccgtggccat ttcagcgagc ctcgcgggag
     4441 ggtggcgcgg ctatactcgg ccagccgttc ttaaccatgg cgtcggggcc cgcgccaggt
     4501 cggcactgtc acttagagtt ggtagaaggg gattggtcga catgccgttg aggtccgact
     4561 ccgagaactt cgctctcatc cgagtgatcg gtatcggagg cggcggctcc aacgccgtta
     4621 accggatgat ccgggcagag ctgatgggtg ttgagttcat ctccgtcaac accgacgccc
     4681 aggcgctcct ccagtcggat gcgccgcaca agatccgcat cggcgacaag atcacgcgcg
     4741 gccttggcgc cggcggcgac cccggcatcg gccagcgcgc ggccgaggaa gatgccgaga
     4801 agatcttcga agcgctcaag gactcggaca tggtcttcat caccgccggc atgggtggtg
     4861 ggaccggctc cggcgcggca ccggtcatcg ccgagattgc gcgtgacctg ggggcgctga
     4921 ccatcggcgt cgtcaccaag ccattcggct ttgagggcgc gcggcggcgc ctcaacgccg
     4981 agaagggcac ggacgcgctg cgcgacaagg tcgacacgct gatcaccatc cccaacgacc
     5041 gcctgcgcga cgtcgtcagc aaggagacgt ccatcctcga cgccttccgc gtcgtcgacg
     5101 acgtgttgcg ccagggggtc cagggcatca gcgacctgat caccgtgccc ggcctgatca
     5161 acctcgactt cgccgacgtg cgcacggtca tgaaggacgc cggctccgcg ttgatgggca
     5221 tcgggcgtgc gagcggcgag ggccgtgccg cgcaagcggc gcgcgacgcc atcagcagcc
     5281 cgctgctcga ggtcaacatc agcggcgcgc agggcgtgct gttcaacgtc accggcggct
     5341 cgagcctgag cctctttgag gtcgatgaag cggcggagat cgtcaaggcg acggccgatc
     5401 ctgaggcgaa catcatcttc ggcacggtca tcgacgagcg gatgggcgac gagatcgcga
     5461 tcacggtcat cgccaccggc ttcgacaaca cgcgccggcg cgagcagacg cacgcccagg
     5521 tgacggagat ggcgcaggtg ctgcgctcgc gcgagcggga gcagcagctc gaccagcgca
     5581 tcgcggcggc cattcccatc tcgacgaacg gcaacggtga ctcgctgccc gcccgcgagc
     5641 cggccgcagc cacggcttcg tcccggacgt cgggcaacga cctgcggcgc gggccgcagc
     5701 aggaggtcga ggacctcgac atcccggcgt tcctgcgccg gcgctcctga gcgaacagcc
     5761 cgacctagct cgccgcct
//
LOCUS       DATBRX010000049        12164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2919584, whole genome shotgun sequence.
ACCESSION   DATBRX010000049 DATBRX010000000
VERSION     DATBRX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12164
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2919584"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VIF08_01680"
     CDS             <1..271
                     /locus_tag="VIF08_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134720.1"
                     /translation="DTDAEGPSTDRLIRDYFGMYRFEWPEEDSVEFHLGYGDMIRLLR
                     SSGLEVEDLVELRPAEGATTRFPYVSAEWSRRWPSEEVWKARKRA"
     gene            complement(268..576)
                     /locus_tag="VIF08_01685"
     CDS             complement(268..576)
                     /locus_tag="VIF08_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134721.1"
                     /translation="MTFQVLAEQYRILLTDPADIAIAQRLLAGDDTAPSIPNGLIVRD
                     DPSVNDGWSWSIDPDSLEFADMTTEVCDGLPSYVEDGSLTGDYFCPWSAEIVALVPAP
                     "
     gene            complement(803..2095)
                     /locus_tag="VIF08_01690"
     CDS             complement(803..2095)
                     /locus_tag="VIF08_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8134722.1"
                     /translation="MAQSLTRSQVRDPLSLRVGGRIREARTRAGLTQQALAGDRYTKA
                     YISALENGLIRPSVKALDYLATRLGTTPGWLMASEAPTWTRLEADLELAAGNWQKAAD
                     AYAELLDASVESVQRAEILRGLAEAHARLDRGAEAVRAAAEAVELFEAAGRDVDAALT
                     SYWLAAGLYYQDNVVESKSVLVAILAKVRAGLNVEPDFKVRLLMALSSNESREGNHQG
                     ALAYLEEVRGLADTLDDRRRAAFLFDLAYSYCESGDFEAAIRAGYASLALYSASNTTT
                     EMAKIENEMALAHLGAGSIGRAAELAASAQQRFAQLGNDRQLAHVLETQAQIAAARSD
                     WVEALRLSQESIDFARKTDNLAAQAAAGLTAARCHVALGDGGAAEDSFAAAADAARQL
                     KRPAVLRRVLSEWADLLAAQGDHERAFALSREALATAQ"
     gene            complement(2061..2354)
                     /locus_tag="VIF08_01695"
     CDS             complement(2061..2354)
                     /locus_tag="VIF08_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8134723.1"
                     /translation="MSTITKHRETASLTLGAEIRRQRRAKRMTQTELGRPLTRAFVSA
                     VEHGRCLPSLAALAHIADRLNVSPADLLEPVKHELAALYTARHGSVADPVPGP"
     gene            2716..3006
                     /locus_tag="VIF08_01700"
     CDS             2716..3006
                     /locus_tag="VIF08_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134724.1"
                     /translation="MEEAAARKNDRTPDSGMFDVPAWLGDLSGEHDWLVSRSFTRRKV
                     EFVIDYTTSHGSNAQVTMRAVTAARSASWELRADLGDSRAGVVIPARTRIKW"
     gene            complement(3025..3975)
                     /locus_tag="VIF08_01705"
     CDS             complement(3025..3975)
                     /locus_tag="VIF08_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134725.1"
                     /translation="MSNLKASDIFTRHPLNPIITADQLPYRANSVFNPGAGLVNGETL
                     LLIRVEDLRGISHLLAARSADGVTNWRFEPTPLIAPEPDTHPEEIWGCEDPRLTWLPE
                     LDQWAIAYTAYSRRGPLVSLALTHDFTNVRKMGPVMPPEDKDAALFPRRIDGRWAMIH
                     RPSPLRGFAHMWVSYSPDLRHWGDHELLLEARDGAWWDAGKIGLGPPPLETADGWLVC
                     YHGVHSTSAGPIYRVGLALLDLDDPRKVLRRTDEWVLGPTAPYERSGDVNKVVFPTGW
                     VVDPVTRVLSLYYGAGDSAIALATADLDAVLDLMRTLPPG"
     gene            complement(3972..5084)
                     /locus_tag="VIF08_01710"
     CDS             complement(3972..5084)
                     /locus_tag="VIF08_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134726.1"
                     /translation="MPDRPLFPARRTHLDRLTGPFGIWQHARGVEPDEAFGTCTDDVA
                     RALTVDLLHRRALGWEAVSSTAHRSVEFLEAAFDQETGTFRNFRHTHGDWLDEAPSHD
                     SLGRALLALGTAARDAPEVALRADAQRLFAAALTAARRLTSPRAIASAVLGCDAALDG
                     GMTGEVQSTFELLTGRLRAVFFDVDLDGDWPWPEETLTYENALLPHALIVAARRLGDA
                     RLRSVGLHVLDWLIAVQTSRRGTFTPIGSDGWWTRGMSRSQFDQQPIEATALILAAAA
                     AYGTTQDAGYLRSAEAAYAWFLGDNDAGLMIADVVTGGCHDGLSDGHVNVNQGAESTL
                     MWLTALETMRQLRTDAMTTRHTRSHGGVPVLVELHA"
     gene            complement(5077..6255)
                     /locus_tag="VIF08_01715"
     CDS             complement(5077..6255)
                     /locus_tag="VIF08_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8134727.1"
                     /translation="MPTTAFVSTYPPQRCGIATFTSDLSAAVGDREVVALQPPDKPGF
                     YPLEVSRRIARDVLPDYLSAAHWINERPFRVVSLQHEYGIWGGQDGAYVLDFVKALRT
                     PVVATLHTVLQNPTPSQRRILTELVHLAAETVVMSTAAASLMTRSYGIDPTRLEIVPH
                     GVPKFPLMAPDGVKSQLGLAGHKVILSFGLLGPGKGYESAIAAMPAVVRADPTAVYVI
                     VGATHPELLRREGEAYREQLMRLAASLGVADRVRFVGRYVAAGELALWLTAADIFVTP
                     YPNLEQIVSGTLSYAMGAGKAIVSTPYAYASEQLAQGRGRLVAAGSTDALADGLIELA
                     LSSALRSDYSRKAGEYSRGMQWPEIGTAYGKIFDRVAGVQHRREDMAGIADRVAIASA
                     "
     gene            6450..6638
                     /locus_tag="VIF08_01720"
     CDS             6450..6638
                     /locus_tag="VIF08_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134728.1"
                     /translation="MDINATVLVEELDARGNVWRVHSSHMVRTNVERCISAIRAGYNM
                     ELTRIRVDGQVVVEPRHR"
     gene            6854..8125
                     /locus_tag="VIF08_01725"
     CDS             6854..8125
                     /locus_tag="VIF08_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEY8134729.1"
                     /translation="MALVRHGVSRRTFLKFSAAIAATLALPMSYAPRIAAAVENAPRV
                     PLIWLRGLACGGDSQAFLQSTNPTVGELLIGALSLDYDEAFMAAAGADATGTLTGLRA
                     RFPEGYIAVVEGSVPTADGGAHATLGGRPMRDVISEVCAGAIGTIAVGSCAFDGGVAA
                     AAGGLTGAVGVGRVINTPHLVALPGCPVNGDNLAATLVHYLTLREFPPTDGLGRPFFA
                     YGGLIHNQCERRAHFEFGEFVQSWGDEASQKGWCLYKMGCKGPQTFANCPTQQYAESS
                     SWNVLAGHGCIGCTMPGFWDGMGGAYQRLPAPLPFLPDVSADAIGLALTGGVAGLAVV
                     HGAASTVRQWRVRRTKKPTPDTTEMTEMTDTTDTTEAAEVTPTAAIETTESAVPAEPL
                     GELEGRPTVDEVWVAEQPLSEPEPVDEEPDL"
     gene            8122..9828
                     /locus_tag="VIF08_01730"
     CDS             8122..9828
                     /locus_tag="VIF08_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEY8134730.1"
                     /translation="MSRIVIDPVTRAGGQLRVEAEVLGGAVTEAWLSATSFRGLESVV
                     RGRAARDAWLVAQRVCGAATGVHALASVRAVENALGVDIPKNARLVRNILAATTLVQE
                     HVVNFYLRELINWADPVAALTADPAATSTLARSITDWPNSDSSWYAAARDRLTALVRP
                     GAGGPLGGAWSGHPAYRLSPEFGLMLYGHYLEALDWQRQLVRVRTILGGKSPHPQTYL
                     VGGMAIAAEWGGPPVPPRGEHPSTRHSPPPLSQQALADMNMLLTNAARFINEAFVPDA
                     LTVAAHYPEWQRLGGGIGHYLSFGEFPVSDSFAGQLLLARGRVMDRDFANPRAIDQGS
                     VAEAVDHAWYDYDATPDALVHPALGQTRPRYAGPALPYSSLGGLDKYSWIKAPRYADD
                     PMETGALARMLVAYGAGNSFVRNAVDGVAFKLDAGMDSLFSTIGRTVARALEAQIVMA
                     RTTEYLRELVANLAQGDLALVNLEGWDMARWPSQASGFGLAEGPGGAVGHWLTIRDGR
                     IADYQIVDGSTWNGSPRDNRGRPGACEQALVGTPVADPNRPLELLRTVHSFALCSACA
                     VH"
     gene            9828..10526
                     /gene="cybH"
                     /locus_tag="VIF08_01735"
     CDS             9828..10526
                     /gene="cybH"
                     /locus_tag="VIF08_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HEY8134731.1"
                     /translation="MQEPERVRLYVWQVPVRVTHWVIVACIVILSVTGVYIADPFLLP
                     AGGNVMTAVRVIHIATAIVFLLAGMVRTLWLLIGNRFARWSAFIPTSRNQATELFRQA
                     GYYAFLRRDIPKVLGHNQLAATAYIALFGLLFIETITGFALDGIGGAQPAGMIFGWLS
                     DLLGPQLIRLIHHLCMWLILAIAIFHIYSCVLVDHIEKNGLMSSIFSGYKFPTREEVL
                     ESRDGGPELLEDAE"
     gene            10523..11284
                     /locus_tag="VIF08_01740"
     CDS             10523..11284
                     /locus_tag="VIF08_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEY8134732.1"
                     /translation="MSLERGIKILIALNAVLVALLAGGLLFNLWNTAAGHPAQAPLPT
                     PTPSQGTMQMGLAHIPTNNDCVLCHEGGGGAAGLKVVPAILHPVEGWRRCTVCHTDTT
                     LGRLAPGHNGIAEEECLNCHKSGQAGPAITQPHAALANQDCLSCHGSVAHLPSSMASS
                     SATDCVLCHKPTLLPPPQFPHSTDPALSCRACHQGEQVGGLPISHALRSDRTCLLCHD
                     ILTEDGTTFPPTVPSPGTQSLLSPTRYPLVLLIAS"
     gene            complement(11578..>12164)
                     /locus_tag="VIF08_01745"
     CDS             complement(11578..>12164)
                     /locus_tag="VIF08_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8134733.1"
                     /translation="YHPGELNVRMADVLVINKVDSASPEVLETLEANLRELNPRATIV
                     RAESELTLVGDPIAGKSVVIVEDGPTLTHGEMTFGAGVVAARRFGAATLVDPEPVSVG
                     SIRAVLEKYPKLEPLVPAMGYGADQTHELEQTLNACDADLVLSATPIDITRVLTLNKP
                     VTRVRYELKQVGGPALEDLLAPIVARARRPAGVA"
BASE COUNT         1957 a         4164 c         4161 g         1882 t
ORIGIN      
        1 cgacacggac gcggaagggc cgtcgaccga tcggctcatc cgcgactact tcggcatgta
       61 ccgcttcgaa tggcccgagg aggacagcgt cgagttccac ctgggctacg gcgacatgat
      121 ccggctgctg cgctcgagcg gactcgaggt cgaggatctg gtcgagctgc ggccagccga
      181 aggcgcgacg acgcgcttcc cctacgtgag cgcggaatgg tcgcggcgct ggcccagcga
      241 ggaagtatgg aaggcgcgca agcgagccta gggcgccggc acgagcgcga cgatctcggc
      301 gctccacggg cagaagtagt caccggtcag cgagccatcc tcgacatacg acggcaggcc
      361 gtcgcacacc tcggtggtca tgtcggcgaa ctcaagtgag tcgggatcga tcgaccagct
      421 ccagccatcg ttgacgctgg ggtcgtcgcg cacgatcaag ccgttgggaa tgctcggcgc
      481 ggtgtcatcg cccgcgagca gccgctgggc gattgcgatg tcggctgggt cggtgaggag
      541 gatgcggtac tgctcggcga ggacctgaaa cgtcacgacg atcggtcctg aacctggcgt
      601 cgggctgggt aagggtgagg gagtcggcgt tgggccggtc gtggggctgg tcgtggggct
      661 gggcgtcgtg gtgccgcagg caaggcacag ggccgcaagg acaaacgtga gcagagccgg
      721 tcgaaactgg gacatgccgc ccagacgcgc gaagagcgtt atgcgttccg ccgggcccca
      781 acgcggcgcg cggccggcga ctctattggg cggtggcgag cgcctcacgg ctgagggcaa
      841 acgcgcgctc gtggtcaccc tgcgcggcca gcagatcggc ccactcgctt aacacgcggc
      901 gtaacacggc cgggcgcttg agctggcgcg ccgcgtcggc cgcggccgcg aaggaatcct
      961 ccgcggcgcc gccatcgccc agcgcgacat ggcagcgggc ggcggtcagt ccggccgccg
     1021 cctgggcggc gaggttgtca gtcttgcgcg cgaaatcaat cgactcctgg ctcaatcgga
     1081 gcgcctcgac ccagtcggat cgagcggcag cgatctgggc ctgcgtctcc aggacgtggg
     1141 ccagctggcg atcgttaccc agctgcgcga accgttgctg tgccgacgcc gccaactccg
     1201 ccgcgcggcc aatgctgccc gcgcccaggt gggccagcgc catctcgttc tcgatcttgg
     1261 ccatctctgt cgtcgtgttg ctcgcggagt acagcgcgag gctcgcgtag ccagcgcgga
     1321 tggctgcctc gaaatcgccc gactcgcagt agctgtaggc caggtcaaac aggaaggcgg
     1381 cacggcgcct gtcgtccagg gtgtcagcca gaccgcgcac ctcttcgaga taggccagcg
     1441 cgccctgatg gttgccctcg cgtgattcgt tcgacgagag cgccatcaac aggcggacct
     1501 tgaagtcagg ctcgacgttc aggcccgccc gtaccttggc gaggatggcc accagcacgg
     1561 acttggactc gacgacgttg tcctggtagt acaggccggc ggccagccag tagctcgtga
     1621 gcgccgcatc gacgtcccgg ccggcggcct cgaacagttc gaccgcttca gcggctgcgc
     1681 ggacggcctc cgcgccgcgg tcgagacgcg catgcgcttc cgccaagccg cgcaggatct
     1741 ccgcgcgctg gacgctttcg acgctggcgt cgagcagctc ggcgtaagcg tcggcggcct
     1801 tctgccagtt gccggcggcc agctcaaggt cggcctcaag ccgggtccat gtgggcgctt
     1861 ccgacgccat gagccagcca ggggtagtcc ccagacgggt cgccaggtag tcaagggcct
     1921 tgaccgacgg ccggatcagg ccgttctcga gcgcgctgat gtaggccttt gtgtagcggt
     1981 cgccggcgag tgcctgctgc gtcaggcctg cgcgagtgcg cgcctcacgt atccgcccgc
     2041 ccacgcggag tgagagcgga tcacggacct gggaccgggt cagcgactga gccatggcga
     2101 gcagtgtaca gcgctgccaa ttcgtgcttg accggttcaa gcaaatcggc cggcgagacg
     2161 ttcaggcggt cagcgatgtg cgccaatgcc gcgagcgacg gcaggcagcg cccgtgctcg
     2221 acggcactga cgaacgcgcg cgtcagtggc cggcccagct cggtctgcgt catgcgcttg
     2281 gcccgtcgct ggcgcctgat ctcagcaccc agcgtgagcg atgccgtctc ccgatgttta
     2341 gtaatagttg acatgcggac cgagcaaccc tagcatctcg gcgggcgccg tgccagatgc
     2401 cgcgtccaca cccacgaggc aggaggggtg ggtgaaagga gcaccaggca tgcacatcag
     2461 cagtctgcgt cgggtccgcc cggcgctcgt ggcctcattc gtttctctgg tcctcagcct
     2521 tatcgcagcc gcgacagctc tcgctgacgg cggcgggacg tccttcccct aggacacccg
     2581 ctcacaggtc gcacgacact tcgtcaactc ttcaggtaga ccaaccaccc ctccaacacc
     2641 tcgcttaacg accccgacca tggaattgac cggcctgtcg gtcggtcaat tgctgctata
     2701 cacttcggcc gacgcatgga agaggcggcg gcgcgcaaga acgacaggac gcccgactcg
     2761 ggaatgttcg acgtgcctgc ctggctgggc gatctgtccg gcgaacacga ttggcttgtc
     2821 agtcgcagct tcacgcgccg caaggttgag ttcgtgatcg actacacgac atcccacgga
     2881 tcgaatgccc aggtcacgat gcgcgcggtc acggccgcca ggagcgccag ctgggagcta
     2941 cgggccgatc tcggtgacag tcgcgcgggc gtcgtcatcc ccgcgcgaac tcgcatcaag
     3001 tggtgacggg cgtcatccgg cgcgtcagcc gggaggcagc gtccgcatca gatcgaggac
     3061 agcgtccagg tcggccgtgg ccagtgcgat agccgagtcg cccgcaccgt agtacagcga
     3121 cagcaccctg gtgacggggt caaccaccca gccagtcggg aagacaacct tgttgacgtc
     3181 gccgctgcgc tcgtaggggg ccgtcggccc gagcacccat tcgtcggtcc ggcgcagcac
     3241 cttgcgtggg tcgtcgaggt cgaggagcgc caggccgacc cgatagatgg gcccggccga
     3301 cgtcgagtgg acgccgtggt agcagaccag ccaaccgtcg gccgtttcga gcggcggcgg
     3361 gcccaggccg atcttgcccg cgtcccacca cgccccatca cgcgcttcga gcagtagctc
     3421 gtggtcgccc cagtggcgca gatcgggcga gtacgacacc cacatgtggg cgaatccgcg
     3481 caggggtgac gggcggtgga tcatcgccca gcggccatcg atccggcgcg ggaagagcgc
     3541 cgcgtccttg tcttccggcg gcatgaccgg gcccatcttg cgaacgttgg tgaagtcgtg
     3601 ggtcaacgcg agcgagacga gcggaccgcg ccggctatag gcggtgtagg cgattgccca
     3661 ctggtcgagc tccggcagcc aagtcaggcg cgggtcttcg cagccccaga tctcctccgg
     3721 gtgagtgtcg ggctcgggtg cgatgagcgg cgtgggctca aatcgccaat tcgtgacgcc
     3781 gtcggcgctg cgcgcggcca ggaggtgcga gataccccgt aggtcctcga ccctgatcag
     3841 cagcagcgtt tcgccattga ccagccccgc ccctgggttg aacacggagt ttgcgcgata
     3901 cggcagctgg tcggccgtga tgatcgggtt gagtgggtgc ctggtgaaga tgtccgacgc
     3961 cttgaggttg ctcatgcgtg caactccacc agcacgggca cgccgccatg agaacgggta
     4021 tgccgagtgg tcatagcgtc cgtgcgcagc tggcgcatcg tctcgagcgc ggtcaaccac
     4081 atcagggtcg actcggcgcc ctggttcacg ttcacgtggc cgtcggacag cccatcgtgg
     4141 cagccgccgg tgacgacatc ggcgatcatg agacccgcgt cgttgtcgcc caggaaccac
     4201 gcatatgccg cttctgccga tcggagatag cccgcatcct gcgtcgtgcc gtaggcagca
     4261 gcggccgcga ggatcagcgc cgtggcctcg atgggctgct ggtcgaactg gctgcggctc
     4321 atgccgcggg tccaccagcc gtcactgccg atcggggtga aggtgccgcg gcgcgaggtc
     4381 tgaacagcga tcagccagtc aaggacgtgt agcccgacgc tgcgcagccg cgcgtcgccc
     4441 aaccgtcgcg cggccacgat cagggcgtgc ggcagcagcg cgttctcgta ggtgagcgtt
     4501 tcctccggcc agggccaatc cccgtccagg tcgacgtcga agaacacggc gcgcaggcgt
     4561 cccgtcagaa gctcgaacgt cgactgcacc tctccagtca tgccgccgtc aagtgccgcg
     4621 tcacagccca gcacggccga cgcgatcgcg cgcggcgagg tcaagcgcct ggcagcagtg
     4681 agcgcggccg cgaacaagcg ctgggcatcc gctcgcagcg cgacctcggg cgcatcccgg
     4741 gcagccgttc ccagcgcgag aagggcacgc cccaggctgt cgtgagatgg cgcctcgtcc
     4801 agccagtcgc cgtgcgtgtg acggaagttc cggaaggtcc cggtctcctg gtcaaacgcc
     4861 gcctccagga actcgacgga tctgtgcgcg gtgctgctca ccgcttccca gccgagagcg
     4921 cgccgatgga ggagatcgac cgtcagcgcc cgggcaacat catccgtaca ggtgccgaag
     4981 gcctcgtctg gctcgacgcc ccgggcgtgc tgccagatcc cgaacgggcc ggtcagacga
     5041 tcgagatgcg tgcggcgagc gggaaacagc ggtcgatcag gcactggcga tggccactct
     5101 gtccgcgatg ccggccatgt cctcgcgacg atgctgcaca ccggctacgc ggtcaaagat
     5161 cttgccatac gccgtcccaa tctcgggcca ttgcatgccc cggctgtatt cgcctgcttt
     5221 gcggctgtag tcggaccgca aggcgctgct cagggcgagc tcgatcaggc cgtcggcgag
     5281 cgcgtctgta gagccggccg cgacaagccg gccgcgacct tgcgcgagtt gctcgctcgc
     5341 gtaggcataa ggcgtcgaca cgatcgcctt gccggcgccc atggcgtacg acagcgtgcc
     5401 agagacgatc tgttccaggt tggggtaggg cgtgacgaat atgtctgcag cggtcagcca
     5461 cagtgcgagc tcgcctgcgg cgacgtagcg gccgacgaaa cggacgcggt ccgccacgcc
     5521 gagggacgcc gcgagccgca tgagctgttc gcgataggcc tcgccttcgc gccggaggag
     5581 ctctgggtgg gtagcgccga cgatgacata cacggcggtc ggatcggctc ggaccaccgc
     5641 gggcatggcg gctatggctg attcgtagcc cttgccggga cccagcagac cgaagctcag
     5701 gattaccttg tggccggcca agcccagttg cgacttgacc ccgtcgggtg ccatcagtgg
     5761 gaacttcgga acgccgtgcg ggacgatctc gaggcgtgtt ggatcgatgc cgtaggagcg
     5821 ggtcatcagc gacgcagctg ccgttgacat gacgacggtc tccgcggcca ggtggacgag
     5881 ctcggtcagg atgcgccgct gcgacggtgt cgggttctgc aggaccgtgt gcagggtcgc
     5941 gaccacgggc gtgcgcagag ccttgacgaa gtcgaggaca taggcgccat cctggccgcc
     6001 ccagatgccg tactcgtgct ggagcgagac gacacggaac gggcgctcat tgatccagtg
     6061 cgccgcgctg aggtagtccg ggagcacatc gcgcgcaatg cggcggctga cctcgagtgg
     6121 atagaagccg ggcttgtcag gcggctggag ggcaactacc tcccgatcgc caacggctgc
     6181 ggacaggtca gaggtgaatg tcgcgatccc gcagcgctgc ggcgggtaag ttgaaacgaa
     6241 tgcagtcgtc ggcatgcagt acctctcgtg gagcgggagt cccgaaccga acagcaggcg
     6301 cacggcccgt tcaggattgg gtcgaccacg cggagggtcc ccgcagacgg ggggacgtac
     6361 ccaccgtagc agatcggccc gaccacactc ccggcgccga atgaccaacg gcactacccg
     6421 cccgcgtcgc cagacgcgac aaaacgcgca tggatatcaa tgccaccgtc ctcgtcgagg
     6481 agctcgacgc gcgcggcaac gtctggcgcg tccattccag ccacatggtc cggacgaacg
     6541 tcgagcgctg tatcagcgcc atccgggcgg ggtacaacat ggagctgacg cggatcaggg
     6601 tcgacggcca ggtcgtggtc gagccgcgcc accgctgagc gagccgcgcc accgctaggc
     6661 gagccgcgcc accgctaaca accgtttggc gggtcagaaa gggaactctg gccctccgcc
     6721 gctgcaccgg tgaaagccga gtattcatcg tggaagggac cggccgttag cttcggagca
     6781 cggactgaaa cccatcccag gcgcgggcgc gaccgagcgg ccggcgcttc ctgatcgcga
     6841 tgagcgcccg tacgtggcgc ttgttcgcca tggcgtctcg cgccgcacgt tcctgaagtt
     6901 cagcgcggcg atcgccgcga ccctggcgct gcccatgtcg tacgcgccgc gcatcgcggc
     6961 cgcggtcgag aacgcgccgc gcgtcccact catctggctg cgcggcctcg cctgtggcgg
     7021 cgacagccag gcgttcctcc agtcgacgaa cccgacggtc ggcgagctcc tgatcggcgc
     7081 gctgtcgctc gactacgacg aggcgttcat ggccgcggcc ggcgcggacg cgaccggcac
     7141 cctgaccggg ctcagggcac gcttccctga gggctacatc gccgttgtcg aagggtccgt
     7201 gccaaccgcg gacggcggcg cccatgcaac gctcggcggc cggccgatgc gcgacgtcat
     7261 cagcgaggtc tgcgccggcg cgatagggac catcgccgtg ggcagctgcg catttgacgg
     7321 aggcgtggca gcagcagccg gcggcctgac cggcgccgta ggtgtcggtc gggtcatcaa
     7381 tacgccacac ctggtcgcct tgcccggctg cccggtcaac ggcgacaacc tggccgcgac
     7441 cctcgtgcat tacctgacgc tcagggagtt cccaccgacc gacggcctgg gccggccgtt
     7501 cttcgcctat ggcggactca tccacaacca gtgtgagcgg cgcgcccact tcgagttcgg
     7561 cgagttcgtc cagtcgtggg gcgacgaggc cagccagaag ggttggtgcc tgtacaagat
     7621 gggctgcaag ggtccgcaga ccttcgccaa ctgtccgact cagcagtacg cggaatcatc
     7681 ctcgtggaac gtgctcgccg gccacggctg catcggctgc acgatgcccg gcttctggga
     7741 cggcatgggc ggcgcgtacc agcggctgcc ggcaccgctg cctttcctgc cggacgtctc
     7801 cgccgacgcg atcggcctgg cgctgaccgg tggcgtcgcg gggctggcgg tcgtccacgg
     7861 cgcggccagc accgtccgcc agtggcgcgt gcgcaggacg aagaagccga cgcccgacac
     7921 gaccgaaatg accgaaatga ccgacacgac cgacacgacc gaagccgccg aggtcacgcc
     7981 gaccgcggcc atcgaaacca cggaatccgc ggtaccagcc gaaccgttgg gcgaactgga
     8041 agggcgcccg acggtcgacg aggtatgggt cgctgagcag ccgctcagtg agccggagcc
     8101 ggtcgacgag gagcccgatc tatgagccgc atcgtcatcg atccggtcac ccgggcaggc
     8161 gggcagctgc gcgtcgaagc cgaggtgctg ggcggtgccg tcacggaagc gtggctgtcg
     8221 gccacctcct tccgcgggct cgaatccgtg gtgcgcggcc gtgcggcacg cgacgcctgg
     8281 ctcgtcgcgc agcgcgtctg tggcgccgcg accggggtcc acgcgctggc gtcggtgcgc
     8341 gcggtcgaga acgcgctcgg ggtcgacatt cccaagaacg cccgactggt acgcaacatc
     8401 ctggccgcga cgacgctcgt ccaggagcac gtcgtcaact tctatctgcg cgagctcatc
     8461 aattgggccg acccggtcgc ggccctgacc gccgatcccg ccgctaccag cacgctcgcc
     8521 cgttcgatca ccgattggcc aaattcggat agcagctggt acgccgccgc gcgcgatcgg
     8581 ctcacggcgc tcgtgcgtcc cggcgccggc ggcccgctgg gcggggcgtg gtcgggacat
     8641 ccggcgtacc ggctgtcgcc ggaattcggc ctgatgctgt acggccacta cctcgaggcg
     8701 ctcgattggc agcgtcagct ggtgcgcgtc cggacgatcc tcgggggcaa gagcccgcac
     8761 ccgcagacgt acctcgtggg cggcatggcg atcgcagccg agtggggcgg gccgccagtg
     8821 ccgccccgcg gcgagcatcc atccacgcgc cactccccac cgccgctcag ccagcaggcg
     8881 ctcgcggaca tgaacatgct gctgaccaac gcggcgcgct tcatcaacga ggcgttcgtg
     8941 cccgacgcgc tgaccgttgc cgcgcactac cccgagtggc aacgtcttgg cggcggcatc
     9001 ggccactacc tgtcattcgg cgaattcccc gtgtcggact cgttcgcggg ccagctcctg
     9061 ctggcgcgcg gccgggtgat ggatcgcgac tttgcgaacc cgcgcgcgat cgatcagggc
     9121 agcgtggccg aggcggtcga tcacgcctgg tacgactacg acgccacgcc cgacgcgctc
     9181 gtgcacccgg cgctgggcca gacccggccg cgctacgccg gcccggcgct gccgtactcg
     9241 tcgctcggcg gtctcgacaa gtactcgtgg atcaaggcgc cgcgctacgc cgacgatccc
     9301 atggagacgg gcgcgctggc gcgaatgctc gttgcctatg gcgccggcaa ctccttcgtt
     9361 cggaacgccg tggacggcgt cgcgttcaag ctcgatgccg gcatggactc gctgttcagc
     9421 acgatcggac ggacggtcgc acgcgcgctt gaggcgcaga tcgtgatggc gcgcacgacc
     9481 gagtacctgc gcgagctggt ggccaacctc gcccagggcg acctggcgct cgtcaacctc
     9541 gagggctggg acatggcccg ctggcccagc caggcttcag gtttcggcct cgccgagggg
     9601 cccggcggcg cggtgggcca ctggctgacc atccgcgacg gccggatcgc cgactaccag
     9661 atcgtcgacg gcagcacctg gaacggatcg ccgcgcgaca accgcggccg ccccggcgca
     9721 tgcgagcagg cgctggtcgg cacacccgtt gccgacccca atcggccgct cgagctgcta
     9781 cggacggttc attcgttcgc gctgtgctcg gcgtgcgcgg tgcactgatg caagaacctg
     9841 agcgggtccg gctctacgtc tggcaggtgc ccgtgcgcgt cactcactgg gtgatcgtcg
     9901 cgtgcatcgt gatcctgagc gtcaccggcg tgtacatcgc cgacccgttc ctcctcccgg
     9961 ccggcggcaa cgtcatgacc gccgtgcgcg tgatccacat cgcgacggcc atcgtctttc
    10021 tgctggctgg catggtgcgc accctgtggc tcctgatcgg caatcgtttc gcgcgctggt
    10081 cggcgttcat ccccacgtcg cgcaaccagg cgaccgagct gttccgccag gctggctatt
    10141 acgccttcct gcgccgcgac atccccaagg tgctgggcca caaccagctc gccgccacgg
    10201 cgtacatcgc cctgttcggc ctgctgttca tcgagacgat caccggcttc gcgctcgacg
    10261 gcatcggcgg tgcgcagccg gccgggatga tcttcggctg gctgtctgac ctgctcgggc
    10321 cgcagctcat ccggctcatc caccacctgt gcatgtggct gatcctggcc atcgcgatct
    10381 tccacatcta cagctgcgta ctggtcgacc acatcgagaa gaacggcctg atgtcgagca
    10441 tcttcagcgg ttacaagttc ccgacgcgcg aagaggtgct cgagtcgcgc gacggcggac
    10501 ctgagctgct cgaggacgct gaatgagcct cgagcgcggc atcaagatcc tgattgcgct
    10561 caacgcggtt ctggtcgcgc tgctcgcggg cgggctgctg ttcaacctct ggaacaccgc
    10621 ggcgggccac ccggcgcaag ccccgctgcc caccccgacg ccctcgcagg gcacgatgca
    10681 gatgggcctc gctcacatcc ccaccaacaa cgactgcgtc ctatgtcatg agggcggcgg
    10741 gggtgcggcc ggactgaagg tcgtgcccgc gatcctgcac cccgtcgagg gctggcggcg
    10801 atgcaccgtg tgccatacgg acacgacgct cggccggctc gcgccaggcc acaacggcat
    10861 cgccgaggag gagtgcctca actgtcacaa gagcggccaa gccggtccgg cgatcaccca
    10921 gccgcacgcg gcgttggcga accaggactg cctctcgtgc cacggctcgg ttgcccacct
    10981 gccttcgagc atggccagca gcagcgccac cgactgcgtg ctgtgccaca agccgaccct
    11041 cctgccgccg ccgcagttcc cgcactcgac cgatcccgcc ctgagctgcc gggcatgcca
    11101 ccagggcgag caagtcggcg gactgccaat cagccacgcc ctgcgctccg accggacgtg
    11161 cctgctctgc cacgacatcc tcaccgagga cggcacgacg ttcccgccca cggtcccgtc
    11221 gccgggtacc caatcgctgc tcagcccgac gcgctacccg ctggtcctgc tgattgcctc
    11281 ctgaggcgca cggccaaatg ctcgtctggc gagcggtaat ggcgcgacaa gccggggcgg
    11341 ctggttatac gctccaatag cgagcgtaat aggcgcctaa ccattcctgg cagcgtcaga
    11401 tgcgcgccac caaagcacta ggccaactcg acgatctgcg agttgacata gcgccccgcc
    11461 tgcgcgcgca aggccgcgtt ggtgccatcc agttgcgtcg atggacgcgg tgtcccgtca
    11521 gttgcggtcc agatgtcggc cgagtgccgg gttaatgctc caagttggag cgtatggcta
    11581 ggcgacgccg gcagggcggc gggcgcgcgc cacgatcggc gccagcaggt cctcgagcgc
    11641 cggcccgcca acctgcttga gctcgtagcg cacccgcgtg acgggcttgt tgagcgtcag
    11701 cacacgagtg atgtcgatcg gcgtggctga aagcacgagg tccgcgtcgc aggcgttgag
    11761 cgtctgctcg agctcgtgcg tctggtcggc cccgtagccc atcgcgggaa cgagcggctc
    11821 gagcttgggg tacttctcca gcacagcgcg aatggaaccc accgacaccg gctcgggatc
    11881 gaccagcgtc gcggcaccaa agcgccgcgc ggcgaccacg ccggcgccaa aggtcatctc
    11941 gccgtgggtc agcgtggggc cgtcctcgac gatgaccaca ctcttgcccg cgatcgggtc
    12001 gccgacgagc gtcagctccg actcagcgcg aacgatggtc gcgcgcgggt tgagctcgcg
    12061 caggttggcc tcgagcgtct cgaggacctc gggcgacgcg ctgtcgacct tgttgatcac
    12121 cagcacgtcg gccatgcgga cgttcagctc gcccgggtga tagc
//
LOCUS       DATBRX010000050         2159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3702124, whole genome shotgun sequence.
ACCESSION   DATBRX010000050 DATBRX010000000
VERSION     DATBRX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3702124"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2143
                     /locus_tag="VIF08_01750"
     CDS             <1..2143
                     /locus_tag="VIF08_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY8134734.1"
                     /translation="PRLQKRPEGAEEGGDFFVDLKEKAVAPTEEGISKIERLLKIDNL
                     FDTDPRLARHFEQALRAHALYQRDRDYIVENGEIVIVDEFTGRKMPGRRWSEGLHQAI
                     EAKEGLRVQRESVTLATITFQNYFRLYQKLSGMTGTAMTEHEEFYKIYGLEVVAVPTH
                     RAMIREDHTDVVYRSEDAKFNALIDEIAEMSEAGRPVLVGTTSVEKSEVLSEMLKRRG
                     IKHEVLNAKFHEKEAPIVAQAGRSGAVTIATNMAGRGTDILLGGNPAGMASAELHRRG
                     INPAEAPPEVYEEALANAKAATAEDHDRVVAAGGLHIIGTERHEARRIDNQLRGRAGR
                     QGDPGSSRFYLSLEDSLMKRFASERVTGLMERMGLQGDVSLEHGLVSRTIESAQTRVE
                     GYNFDIRKRVVEYDDVINKQRETIYAERDKVLNNEDLTETVRNFIEGEIEALVDGHLA
                     GPEQDWDYDGLARELAQMGLVGDDLDGDGLAEFTEREGLLEHLRDAVDRTLEQREQQL
                     GADVWAQVERLVLLRSIDSLWVDHLTELDDMRRGIGLRGYGGTDPLNEFKREAFKLYE
                     ELRGFISRQVANTIFRVQVTQQAPAQTFPMPVPDQFNSTGTDGNGSAADRITPVEGGH
                     VHSDGTFHAGEAPAPGPGVAPQVGAAAGVALSGLKGERPREMQYSAGEQVAAAGAARG
                     AIPKAAAGPKLGRNDLCYCGSGKKYKRCHGA"
BASE COUNT          410 a          709 c          753 g          287 t
ORIGIN      
        1 gccgcgactc cagaagcggc ccgaaggagc ggaggagggc ggcgacttct tcgtcgatct
       61 caaggagaag gccgtcgccc ccacggaaga gggcatctcc aagattgagc ggctgctcaa
      121 gatcgacaac ctcttcgaca ctgatccacg cctggcgcgc cacttcgaac aggcgctgcg
      181 cgcccacgcg ctctaccagc gcgaccgcga ctacatcgtc gagaacggcg agatcgtgat
      241 cgtcgacgag ttcaccgggc gcaagatgcc cggccggcgc tggtcggagg gtctgcacca
      301 ggcgatcgag gccaaggagg ggctgcgcgt ccagcgcgag tcggtgacgc tcgccacgat
      361 caccttccag aactacttcc ggctttacca gaagctgtcg ggcatgaccg gcacggccat
      421 gaccgagcac gaggagttct acaagatcta cggcctcgag gtggtcgcag tcccgaccca
      481 ccgggcgatg atccgcgagg accacaccga cgtggtgtac cgcagcgagg acgccaagtt
      541 caacgcgctc atcgacgaga tcgcggagat gagtgaggcc gggcggccgg tgctggtggg
      601 cacgacgagc gtcgagaaga gcgaagtcct gtccgagatg ctcaagcggc gcggcatcaa
      661 gcacgaggtg ctcaatgcca agttccacga gaaggaggca ccgatcgtcg cccaggcggg
      721 ccgctcgggc gcggtgacca tcgcgaccaa catggcgggc cgcggcacgg acatcctgct
      781 cggcggcaac ccggcgggca tggcctcggc cgagctccat cgccgcggca tcaacccggc
      841 tgaggcgccg cccgaagtct acgaagaggc gcttgcgaac gccaaggcag cgaccgcgga
      901 ggaccacgac agggtggtcg cggcgggcgg tctgcacatc atcggcacag agcgccacga
      961 ggcgcggcgc attgacaacc agctgcgcgg ccgcgccggc cgccagggtg acccgggctc
     1021 gtcgcgcttc tacctgtcgc tcgaagacag cctcatgaag cgctttgcgt cggagcgggt
     1081 gacgggcctg atggagcgca tgggcctcca gggcgacgtt tcgctggagc acggcctcgt
     1141 ctcacgcacg atcgagagcg cccagacccg ggtcgagggc tacaacttcg acatccgcaa
     1201 gcgcgtcgtg gaatacgacg acgtcatcaa caagcagcgc gagacgatct acgccgagcg
     1261 cgacaaggtc ctcaacaacg aggacctgac cgaaacggtc cggaacttca tcgaaggcga
     1321 gatcgaggcg ctcgtcgatg gccacctggc cgggccggag caggactggg attacgacgg
     1381 cctggcgcgc gagctcgccc agatgggcct ggtcggcgac gatctcgatg gcgatggcct
     1441 ggccgagttc accgaacgcg aaggcctgct cgagcacctg cgcgacgcgg tcgatcgcac
     1501 cctggagcag cgcgagcagc agctgggcgc ggacgtgtgg gcccaggtcg agcggctggt
     1561 gctgctgcgc tccattgatt cgctgtgggt cgaccatctg accgagctcg acgacatgcg
     1621 gcgcggtatc ggcctgcgcg gctacggcgg caccgatccg ctgaacgagt tcaaacgcga
     1681 ggccttcaag ctgtacgagg agctgcgcgg cttcatcagc cgccaggtcg cgaacacgat
     1741 cttccgcgtg caggtcacac agcaggcacc ggcgcagacc ttcccgatgc cggttccaga
     1801 ccaattcaac tcgaccggga cagacggcaa tggatcggcg gcggaccgga tcacgcccgt
     1861 cgagggcggg cacgtccatt cggatgggac gttccatgcc ggcgaggcgc ccgcgccagg
     1921 gcccggcgtg gctccgcagg tcggcgctgc agccggtgtc gcgctgagcg gtctcaaggg
     1981 cgagcggccc cgtgagatgc agtactcggc aggcgagcag gttgcggccg ctggggcggc
     2041 gcgcggcgcc atacccaagg ccgccgccgg acccaagctg ggacgcaacg acctctgcta
     2101 ttgcggcagc ggcaagaagt acaagcgctg tcacggggct tgagcaagac caagctcca
//
LOCUS       DATBRX010000051         4018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_15723, whole genome shotgun sequence.
ACCESSION   DATBRX010000051 DATBRX010000000
VERSION     DATBRX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15723"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VIF08_01755"
     CDS             complement(<1..200)
                     /locus_tag="VIF08_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134735.1"
                     /translation="MGSNSERTKFMLGEEQIPRAFYNIVADLKEPPPPPLHPGTGQPI
                     GPADLAPLFPMELIKQVVSTDR"
     gene            complement(607..1203)
                     /gene="thpR"
                     /locus_tag="VIF08_01760"
     CDS             complement(607..1203)
                     /gene="thpR"
                     /locus_tag="VIF08_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY8134736.1"
                     /translation="MSSQTGRPDRDAWRLFVAAPMPAAAAEEAAAAMAALRGRHPDTR
                     WLPPHKFHLTLVFLGQTASRDVQRIAAVTAAVAQRHRAYQVVTGDGGGRVHSQRGGVC
                     WLRLAEGGSETAQLALDLDADLGSATYDATHTPHPHLTLARGIDRATLEDVRSTARGL
                     TLRWTADRVVLFRSYTDPAGSIYEELADFALRGSRTVD"
     gene            complement(1206..1976)
                     /locus_tag="VIF08_01765"
     CDS             complement(1206..1976)
                     /locus_tag="VIF08_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8134737.1"
                     /translation="MSRSATGLARPEVLFLDVGDTLIRAHPSWAGVYRQGLLESGIDI
                     GEKDLERALLQETQAGGWWLDETPFEPSEVKSFETVVAFDAAVLARLGYPDLAEDTFR
                     RIEDAFARRSAWYVFPDVNPALDALTGAGIRLCVISNFVWGAPELIHDLELARHFEKL
                     VISARVGFQKPNPGIFRHALEQMGVAPERAMHVGDSYRADVMGARRLGIAAALIARGL
                     DDPVRLRDEHSDPDLVVLTDLNDLLELFAIERPVASPA"
     gene            2049..2726
                     /locus_tag="VIF08_01770"
     CDS             2049..2726
                     /locus_tag="VIF08_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134738.1"
                     /translation="MNGRRARERRRRHDDESGIVMPRPEEVRAALDGLSPELPWSELR
                     DQLRPVFIRRRPLPPGMTAPVVRRLPPGLDVALGADIGPAFLYVSAPLLDSWSVSETE
                     AFEIALSNLRNVIEAEGHFEMAYEPIDDVPVWWYQSHGGLASGLLLLEDVIVERYGDQ
                     PRLLMAPMRNLLLAAPHDADRALMSWLRDEISAADPNGLDLPLFSLVDGRLSIDAVPH
                     GRRALVH"
     gene            2756..3103
                     /locus_tag="VIF08_01775"
     CDS             2756..3103
                     /locus_tag="VIF08_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY8134739.1"
                     /translation="MDEEQFETTVPPSGEGCVECLATPDGWWFHLRRCARCGHVGCCD
                     SSPSQHGSKHAHLTGHRVAQSFELGEDWFWDYFEDDFVDGPDLAPPHAHPVTQPAPGP
                     AGRVPDDWQDKLH"
     gene            complement(3131..>4018)
                     /locus_tag="VIF08_01780"
     CDS             complement(3131..>4018)
                     /locus_tag="VIF08_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8134740.1"
                     /translation="RSGELMMIGGAQGTGKTTMALQIARNIAQSGQANVLYICFEHDE
                     EYLLNRLMAMESALAGAMPPDRNRSIRIQDVRREVLGTWLAQGGQDNADLRGNPRLRP
                     ALERISRYGQGLFLLRGSHAHTTAPNLRTMIEGYRRVAPGRPLVVFIDYLQRVPAVPD
                     PPTETERVTNVVAALKDLALGLGVAVVCIVAADKEGLKAARLRNHHLRGSSALNYESD
                     VILILNEKYNIVAKVNIEFNPHQAQRFRDWTILTVEKNRSGRDAVDLEFEKHFEFSCF
                     DPEGRQVVEKLIEERLYND"
BASE COUNT          607 a         1356 c         1387 g          668 t
ORIGIN      
        1 tccctatcgg tgctcacgac ctgcttgatg agctccatcg ggaagagcgg cgccaggtcc
       61 gcgggaccga tgggctggcc cgttccggga tggagcggtg gtgggggcgg ctccttgaga
      121 tccgccacga tgttgtagaa ggccctgggg atctgctcct caccgagcat gaacttggtg
      181 cgctcgctgt tcgagcccat ttcggtggta cctccgcttt gcttcggcct cgttccgggg
      241 ccgaaaacac gtaaccggcc ttcgtcctgc cgggacgaaa ggccggtcct tccgcggtac
      301 cacccgtttt cggcggtcaa ctgatgtgtg cccgccgcac tcgcctgccg acggagccac
      361 acgctcgatc ggtgccgccc tgataacgct ggcgctctgc gccggagcct actcgctcga
      421 ctcgccccct ccgccgcatt ggcagtcaag ccgaactttc ggtccggggc tcacggggcc
      481 attcgccttt gccttcgtcc cggctcacac caaccgccgg gtctctgcat cgaagggccg
      541 ccggctacta atcccgttca aagcccgtat tcagttgagc gacgagtcta ggcagactga
      601 cggccgctag tcaaccgttc gagatccgcg cagggcaaaa tcggccagct cctcgtagat
      661 ggatcctgcc gggtcggtat atgaacggaa caacacgacc cgatccgcgg tccagcgcaa
      721 ggtcaggccg cgcgctgttg agcgaacgtc ctcgagcgtg gcccggtcga tgccgcgcgc
      781 cagggtcagg tggggatggg gcgtgtgggt ggcgtcatac gtcgcgctgc cgagatctgc
      841 atcgagatcg agcgccagct gtgctgtctc actgccgccc tccgccaggc gcagccagca
      901 cacgccgccg cgctgggagt ggacgcggcc gccgccatcc ccggtaacga cctggtaggc
      961 gcgatgccgt tgggcgacgg cggcggtcac tgccgcgatg cgctggacgt cgcgcgacgc
     1021 ggtctggccc aggaagacaa gcgtcaggtg gaatttgtgc ggcggcagcc agcgcgtgtc
     1081 ggggtggcga ccccgcagtg cggccatggc ggccgctgcc tcctcggccg cggccgcggg
     1141 cattggtgcg gcgacgaaca gccgccacgc gtcgcggtcg gggcggccgg tttgactgga
     1201 aatggctagg ccggtgacgc gacgggccgc tcgatcgcga acagttcgag gaggtcgttg
     1261 agatcggtca gcaccacgag gtcaggatcg gaatgctcat cgcgcagccg caccgggtcg
     1321 tccaggccgc gcgcgatgag cgcggcggca atgccgagcc gccgcgcccc catgacgtcg
     1381 gcgcgatacg agtcaccgac gtgcatcgcg cgctctggcg cgacccccat ctgctcgagc
     1441 gcgtgccgga agatgcccgg gttgggcttc tggaagccga cgcgcgcgct gatgacgagc
     1501 ttctcgaagt ggcgcgccag ctccaggtcg tggatcagct ccggcgcgcc ccacacgaag
     1561 ttgctgatga cgcacagccg gatgcccgcg cctgtcaggg cgtcgagggc cgggttgacg
     1621 tccgggaaga cgtaccaggc ggagcgtcgg gcgaaagcgt cctcgatgcg gcgaaaggtg
     1681 tcctcagcca gatcgggata accaagccgc gccagcacgg ccgcatcgaa ggccacgact
     1741 gtctcgaacg acttgacctc gcttggctcg aacggcgtct cgtcgagcca ccagccaccg
     1801 gcctgtgtct cctgcagcag cgcacgctcg aggtcctttt cgccgatgtc gatgcccgat
     1861 tcgagcaggc cctggcggta gacgccggcc cacgacgggt gggcgcgaat gagcgtgtcg
     1921 cccacgtcca gaaagagcac ctcgggccgt gccaggcccg tcgcgctgcg actcatctcc
     1981 actcgcgcaa gtctacggtg agcggctgat aaggcgccgc caagtggcgc gaatcaacat
     2041 ggccggccat gaacggacgg agggcgcgcg agcgtcggcg gcggcatgac gatgagtcgg
     2101 gcatcgtgat gccgcgcccc gaggaggttc gcgcagcgct cgacggcctg tcgccggaac
     2161 tgccgtggtc ggagctgcgc gatcagctgc ggccggtctt catccgtcgt aggccgctgc
     2221 caccgggcat gaccgcaccg gtcgtgcggc ggctgccgcc cgggctggac gtcgccctcg
     2281 gcgccgacat cggcccggcc ttcctgtacg tcagcgcacc cctgctcgac tcgtggtcgg
     2341 tcagcgagac cgaggcgttc gagatcgcac tctcgaacct gcgcaatgtc atagaggccg
     2401 agggccactt cgagatggcg tacgagccga tcgatgacgt gcccgtgtgg tggtaccagt
     2461 cgcacggcgg cctcgcgtcg ggcctgctgc tgctggagga cgtgatagtc gagcgctacg
     2521 gcgaccagcc gcggctcctc atggcgccca tgcgcaacct gctcctggcc gcgccccacg
     2581 acgcagaccg cgcgctcatg tcgtggttgc gcgacgagat ctcagccgcg gatcccaacg
     2641 gcctcgatct gccgctcttc tcgctggtgg acggcaggtt gagcatcgat gcggtgcccc
     2701 atgggcggcg agcactggtg cactagcggc gcgcaacccg tagcatccgg cgggcatgga
     2761 cgaggaacag ttcgaaacca cggtgccgcc cagcggcgag ggctgcgtgg agtgcctcgc
     2821 cacgcctgat ggctggtggt tccacctgcg gcgctgcgcc aggtgcggtc acgtgggctg
     2881 ctgtgactca tcgccctcgc agcacggctc gaagcacgcc catctcacgg gtcaccgcgt
     2941 cgctcagagc ttcgagctag gcgaggactg gttctgggac tacttcgagg acgacttcgt
     3001 ggatgggccc gacctcgcgc cgccgcacgc tcatcccgtg acgcagcccg cacccggccc
     3061 ggcgggacgg gttccggacg actggcagga caagctccac tagcgctctg cccggctgaa
     3121 gcccggcagg tcagtcgttg tagaggcgct cctcgatcag cttttccacg acctggcgcc
     3181 cttccgggtc gaagcacgag aactcgaagt gcttctcgaa ttcaaggtcc accgcgtcac
     3241 gacctgagcg gttcttctcc acggtcagga tggtccagtc gcggaagcgc tgtgcctggt
     3301 gcgggttgaa ctcgatgttc accttggcga cgatgttgta cttctcgttc aggatcagga
     3361 tgacgtccga ttcgtagttg agggccgatg acccgcgcag gtggtgattg cgcagtcggg
     3421 ctgccttcag gccctcctta tcggcagcga cgatgcacac gaccgcgaca cccaggccca
     3481 gcgccaggtc cttgagcgcg gccacgacgt tcgtcacgcg ctcggtctct gtcggcgggt
     3541 cgggcactgc cgggacgcgc tgcaggtagt cgatgaagac aacgagcggc cggcccggcg
     3601 ccacgcggcg atagccctcg atcatcgtgc gcaggttcgg cgcggtggta tgcgcgtgcg
     3661 agccgcgcag caggaacagc ccctggccgt agcggctgat gcgctcgagg gccggccgca
     3721 ggcgcgggtt gccgcgcagg tccgcgttgt cctggccgcc ctgggcgagc cacgtgccca
     3781 gcacctcgcg gcgcacgtcc tggatgcgga tgctccggtt cctatccggt ggcatggccc
     3841 cggcgagcgc ggactccatg gccatcagcc ggttgagcag gtattcctcg tcgtgctcga
     3901 agcagatata gagcacgttg gcctggccgc tctgcgcgat gttgcgcgcg atctggaggg
     3961 ccatcgtggt cttgccggtg ccctgggcgc cgccgatcat catcagctcg ccgctgcg
//
LOCUS       DATBRX010000052        21541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2026918, whole genome shotgun sequence.
ACCESSION   DATBRX010000052 DATBRX010000000
VERSION     DATBRX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21541
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026918"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1868
                     /gene="purL"
                     /locus_tag="VIF08_01785"
     CDS             <1..1868
                     /gene="purL"
                     /locus_tag="VIF08_01785"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY8134741.1"
                     /translation="TRGLVNGVVHGVGGYGNCVGVPTIGGELTFDPTYTGNPLVNVMC
                     VGVLPEDRIAHAAAPTPGSLAVLYGSTTGRDGIGGASVLASANFDEASAQMRPSVQVG
                     DPFAGKLLIETTLELVERGLLEGLQDLGAAGLTCAASELADRGGTGMRVDLAAVPRRE
                     AGMAPFEVMISESQERMLAIVTPARLDEVLEVCRRWGLPAAVVGEVTGDGDVVVVDAG
                     QEIARVPARALASESIEIPRLSSPPERRRSAPAPGETPLAHDGLPERGMDPAAVLEAL
                     LGHSNLGSRAWVTTQYDQTVGADTVAGCERAAGMLRIKGTKRAIVVATDSQPAVALHD
                     PALAASLAVAECARNIAVTGARPLGITNCLNFGDPGVPDAYWQLEQSVRGLRDAAIAL
                     AIPITGGNVSLYNESPLGRIAPTAQIGVVGVIDDIATLVAPAFKSEGDIVVLLGEGGP
                     GLVGSTYELIAGAAPDDRPPSLEMTRELALHEVLRRAAAARLLESAHDVSGGGLAVAI
                     AEAAIWGDLGADLQVAVALAPAVVLFGESPSRAVVTIKPERWSALVKVASGLRVACRK
                     LGSVGGSRLRIRLVGVGATGAAEERGAGVADELDVALTDLRRAWDQALPRVMGEG"
     gene            1883..3310
                     /gene="purF"
                     /locus_tag="VIF08_01790"
     CDS             1883..3310
                     /gene="purF"
                     /locus_tag="VIF08_01790"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY8134742.1"
                     /translation="MVGVHAPGHEAAPLATTALFALQHRGQESAGIAVTDGRGVMVYK
                     DLGHVDQVLQEHRLESLRGGLAIAHCRYSTTGSTIWENSQPTLRMGPRRTVAVGHNGN
                     LVNTRELLAALPGGKARLVGTTDTELLTALLALEPAEDLVEALLALLPRVSGAYSLVV
                     MDEGRVIGVRDPYGFRPLVLGTLAMPDGATGYVLASETAALDVLGAEFVRDVEPGEMV
                     VLGEPGGPRSVRFAEGRENLCVFELIYFARPDSYMLGRNLYEARRRMGEALAREAPAD
                     VDLVMPVPDTGAPAAAGYAEASGIPYREGMVRNRYAGRTFIQPSNGLRQRGVLVKLSA
                     LREQVRDKRLVVVDDSIVRGNTTRQIVGLLRRAGAREVHVRISAPPIYHPCFYGIDTQ
                     IETELIAAAKSVEEIRDFIGADSLSYLSIGGVLDAINLPRERFCFACFDGQYPVPVPY
                     DPGNHKLVLERDEEPVAAAVAVVGR"
     gene            3307..4278
                     /gene="purM"
                     /locus_tag="VIF08_01795"
     CDS             3307..4278
                     /gene="purM"
                     /locus_tag="VIF08_01795"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY8134743.1"
                     /translation="MTRTAYAQAGVDIDAGERAVALMRRHAQSLSGEFASIAAVPPGM
                     REPLVVSATDGVGTKTDIARRLGRYDTIGQDLVAMCADDVVCHGAAPSFFLDYVAVGR
                     ILPERVAEIVGGIAAACAEIGCELLGGETAEHPGLMGHDEFDLAGFCVGFVERDAVID
                     GSAARAGDVVIGLASSGLHSNGFSLIRRLVDDGRLALTDELLTPTRLYARAVLGVGGV
                     HGMAHITGGGLARNLPRGVGPALGVRVDPTSWRQPAIFDTVAHAVGMDGAEMRATFNC
                     GIGFTLIVEPDAAATVVGHLEARGIEAWRIGHVRPVDELGGSRYVEA"
     gene            4275..6455
                     /gene="purH"
                     /locus_tag="VIF08_01800"
     CDS             4275..6455
                     /gene="purH"
                     /locus_tag="VIF08_01800"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY8134744.1"
                     /translation="MTGRIAVGVSGAGSNLRALRRAEKRGLLGGTIGLVFADRPCPGL
                     EFAAAEGIATALAAPSDYPDRASWDAALAQTLIGASVDWVVLAGLMRVLGDRTLGAFP
                     ERILNIHPTLLPSYPGAHPVRDTLAGGARITGVTIHIVDSSLDGGPIVAQQPLPLPQG
                     EALTDLLPRIRALEHQLLPRVVALALAGGISIDDGRVLIDEAIAGELPRPRRVLLSVS
                     DKSGLVELGRSLADLGFDLVSTGGTARALRDAGVDVIDVASVTGFPEMLDGRVKTLHP
                     RVAAGVLADVRSAEHRRQLAAAAIDPFELVVVNLYRFAEAAARPNIDDDGLIEEIDIG
                     GPTLVRASAKNHANVGIVTDPAQYEDVVAELRDRGRLSDELRRELAAAAFRLTATYDG
                     LIADTLSRRWGTGTEQPGRMSLDLERAEELRYGENPHQSAALYLARGVDLAPGAFANG
                     PLLQQGKPLSYNNILDTAAATGLARDLRGAACVIVKHANPCGVAEADDLAAAWEGALA
                     GDPVSAFGGVVALTGAVDAALAEQLTSIFLEVVAAPEVTREAREILARKPNLRLLLDP
                     GLGAPPLPGMELRSAGGALLMTQADVAPDDPQSWTAATRRAPTDEERAALDLAWRVCR
                     HVKSNAIVLVKGRAVVGVGAGQMSRVDSARLAVAKAGPERAAGAVCASDAFYPFPDAL
                     EVCAGAGVTAFVQPGGSQRDAEVIAAADAAGAAMLMTGTRHFRH"
     gene            6491..6868
                     /locus_tag="VIF08_01805"
     CDS             6491..6868
                     /locus_tag="VIF08_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134745.1"
                     /translation="MDENMNPNNEPMADAERPRAGREMLVQLQQMIDTLATQAAPVMR
                     EVAAKAAELAAIAGEKAGPLAHRAAGMTESAGQKIAARSKEVAADLRRPRDTADPLAD
                     PMADASPDAPPDAERSEHESVGE"
     gene            complement(6941..7138)
                     /locus_tag="VIF08_01810"
     CDS             complement(6941..7138)
                     /locus_tag="VIF08_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134746.1"
                     /translation="MTKAKETKKKVAEKPGQESDVEGHNIFATHDYYTTRNIERRSEL
                     DRDIRDRQRAKEAENRKKGQR"
     gene            7299..8957
                     /locus_tag="VIF08_01815"
     CDS             7299..8957
                     /locus_tag="VIF08_01815"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8134747.1"
                     /translation="MGVIAAYRRVLGNGALSRLLFGEFVSSIGDWLYLVALLVLVWDA
                     TGGDTLALGLIGAARILPYVLLSIPAGIVADRFDRRKILLFTDIVRGLLMLVIAAAVY
                     LELSIWIIVALAITATCFSAFFGPAIGAYLPSLVRDESELGPANSAWSSLVNLAFFIG
                     PAVAALLLSIGSLVAAFVLNSLTFAIVAIVLLGLPSKQPGVKTEAAADAAEPTKAQPL
                     MNALRPISRPLLGLTVLNMVVGFVWGGMGVVTVILSVEVFHIDEATGTGLLNSAIGVG
                     GVVGALIAGALVLRRRQGPPLVVGAAALGVGLALLGQVPDFALALAALGFASAGALLV
                     EIVMTTLLQRIVPDAVRGRALGLIDTVYVLFYAAGAFAIPIIASGQAGLVLLVSGIAI
                     AVSGVVGLVLLGAYAVQQPPDDLRRRLGDVALFAGLPPGRIETAMRHATLRDVKAGTT
                     IIRQGDPADFFYVIGNGRVEVAQTDANGTSRVLRQMSGGEFFGEIGLLSRVPRTATVV
                     ALSDCTLVALEGAAFLELVESGPGLTYKLLDVHRGTVSATGEVS"
     gene            8960..9652
                     /locus_tag="VIF08_01820"
     CDS             8960..9652
                     /locus_tag="VIF08_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8134748.1"
                     /translation="MDDTFAVEVLARCPLFAHGSRSILESLAGTLRRRRFRRGEVIMH
                     QGDPGDSLHIVAAGAVKILLPSAEGEEAIIATLRPGDFFGELALLDGAPRSATATALE
                     ASDTLVLPRSVFMELLDTLPGLRDALLAGLAHELRRLTGHVEELHFLDLAGRLAMRLS
                     RLALEAAPDAHGEIRLEWPYTQSDLAAMIGGTRQSVNRLLSELVEDGLIRIEPDALVI
                     GNLDELARRAER"
     gene            9815..10051
                     /locus_tag="VIF08_01825"
     CDS             9815..10051
                     /locus_tag="VIF08_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134749.1"
                     /translation="MPRPIRPAKMFRAEGVSPVDEDTEGHRMGINSAKGALGAKGALG
                     AKGALGAKGALGAKGALGAGGDDEDTEGHRLSKD"
     gene            10072..10845
                     /locus_tag="VIF08_01830"
     CDS             10072..10845
                     /locus_tag="VIF08_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8134750.1"
                     /translation="MTDPGLTAALEALRQAARRSSVARRLDADTEQALLQTIVEATVM
                     LFEAEASSIALFERDPDRLEFRVAAGAQGAGAIGLSVPPSKGIVGFVFSTGQPLALSD
                     VLADPRFDKTVAEKTGYVPRSIAAVPLVDAETTVGVLQVLDKHTSPTFSLRDMELLAV
                     FARQAAAAIRAARVQRDSQRLLRAALAGVGGADVSDEQLDILISAATTELDTDPDTPF
                     WRLVDRVARMRDLSDRELRIVSDILDVLATHAGRGRRSV"
     gene            10856..11671
                     /locus_tag="VIF08_01835"
     CDS             10856..11671
                     /locus_tag="VIF08_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8134751.1"
                     /translation="MTELDLPAWSEPFTDDERHNLVRAATLKQLNRKWAWGGATGKGV
                     KVAILDSGVEGSHPVVQGRLVESVAVEIVDEEATVVPDEPVDLYGHGTACAGIIVGLA
                     PEAEIYSVRVLGSDLKGKGVAFLRGLEYAVEKGVQVMSLSLSSKSERLFPFFHEVVDE
                     AYFRHVCLVSAVNNVPGLSYPSTFSSVFSVAAHAIPEPETFFYNPSPPVEWGAWGVDV
                     PIGWKDGATTVATGNSFAAPHVAGFVARILSKHPGLTPFEVKAVLAAIANDPG"
     gene            complement(11748..11966)
                     /locus_tag="VIF08_01840"
     CDS             complement(11748..11966)
                     /locus_tag="VIF08_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134752.1"
                     /translation="MSRIPGALDFNPSFYHQEALHEAELRRKEHAARAAQAHHGTHAV
                     ETVVGNFRQHLVAVAASVVTVLGVVALI"
     gene            12172..13758
                     /gene="metG"
                     /locus_tag="VIF08_01845"
     CDS             12172..13758
                     /gene="metG"
                     /locus_tag="VIF08_01845"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY8134753.1"
                     /translation="MTADGARERFYLTTAIFYPSARPALHSLFEAIGADAIVRYQRML
                     GKDVRFLTGMDEHSANVERKATEEGVTPRDLVDPWAATWRAAFDKFGISYDRFIRTTD
                     ADHARASTEMVTRAMASGDIYKGTYSGWYCPGDNEFKTDQQLVDGRCPDHPSLELQWL
                     EEENWFFRLSAYQDRLEQLYRDNPSFCEPEHYRNEVLGWLKDGLRDFSISRAGGQWGI
                     PFPTDPDHRIYVWFDALTNYITGAGFPDDLDAFARWWPVDVHVIGKNITRFHCLYWPA
                     MLMSAGIALPKQVFAHGFMLDRGGAKMSKTQGNVLDPDAMAELMSIDGVRYAVLREVP
                     FDRDADVSYDSFVRRYNADLANDFGNLLNRTLTMTSRFLDGERPAPATTGELGSAWAT
                     AWGSYTGQMDSYLLHKALEALWDFVGHANRFVDDEQPWALNKSAKAGDAEAADRLRST
                     MADLLEACRVVALAGAPFMPGAAQSAHSQLGLDYTYAANGSDGPPLAEQATWGALGGG
                     GKVGAQDILFPRVEIEAPGS"
     gene            13781..14155
                     /locus_tag="VIF08_01850"
     CDS             13781..14155
                     /locus_tag="VIF08_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8134754.1"
                     /translation="MAHGQLTHLEIPADDLPRARRFYSELLGWEIGDMPEFADYPLFS
                     FGAIERAGGALGLRGKTVGNQLRVYFDVDSIDQVLPKVARLGGKVVEPKTEIPNMGWY
                     AVIDDTEGNQVALYQGMPQPAM"
     gene            14152..14934
                     /locus_tag="VIF08_01855"
     CDS             14152..14934
                     /locus_tag="VIF08_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8134755.1"
                     /translation="MRLIDSHCHLQAGAFATDVADVLASAREAGVERVLAPGYDLESS
                     RASIELARTLGVDASVGIHPHVADGSEADWPKIAALAADPLVVAIGETGLDYDRGFSP
                     REAQIANLRRHLRLAAETNKPAILHCRSKPGQRDAQDDLLRELADASAPPALLHSFSG
                     PLDYAERAIDMGVAMSFSGLVFRTGEEASAEVARITAADRLLVETDSPYLSPPGAPRR
                     RNEPRFVEITARWLAEQRGDDPEELGARLVANYDRVFSRPPS"
     gene            complement(14915..15673)
                     /locus_tag="VIF08_01860"
     CDS             complement(14915..15673)
                     /locus_tag="VIF08_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HEY8134756.1"
                     /translation="MTVERRIERPYRVRFDEAGPDGYLRASGFLRFAQDLAWVHSESA
                     GFDRDWYRERRLLWLARAIELDLLAQVPYSHDLDVSTELTGFRRVWARRRSEFKPAGE
                     ERIIAVAITDWVLITEEGRPVRPPQEILDVFGSDFGEFTPMRMEAPTAPESASAREFR
                     PRRSELDPMAHVNNAAYLDYMDEQFLGLERIRHGLPVPRRYRAEFLWSAEAGAKVIGR
                     GWSEHSAWLYQLWGDERELFRARLETDPSSWVGG"
     gene            complement(15670..16599)
                     /locus_tag="VIF08_01865"
     CDS             complement(15670..16599)
                     /locus_tag="VIF08_01865"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEY8134757.1"
                     /translation="MTFSIVARDPATGDLGIAVASKFLAVGAVVPHARAGVGAVATQA
                     LANVRYGADGLALLAAGKSATDVLAELTSNDEGRDHRQAGIVDAAGGSATYTGSGCIT
                     WAGGRTADGVAAQGNLLAGPSVVDALIETFLASTAAFPERLLAGLEAADDQGGDRRGR
                     ESAALLVVREGGGYGGGSDRWIDLRVDDHPDPMREVARLLDLNHLYLDRPAATDLVPI
                     DESLANELRQRLTAVGYAPDKLDTSNSLAEIVERSGLTRTGEPRDLPANWDASWTGAL
                     DEWMSIENLEERAAAGGWIDPRVLEFMREKSPA"
     gene            16981..17289
                     /gene="groES"
                     /locus_tag="VIF08_01870"
     CDS             16981..17289
                     /gene="groES"
                     /locus_tag="VIF08_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8134758.1"
                     /translation="MATATATKLRPLGDRVVIQPTPREEMTKSGIVLPDTAKEKPQEG
                     KILAAGPGRLTDEGKREPMDVKEGDVVLYAKYAGTEFKVEGEDLLIVSQKDILAIVNS
                     "
     gene            17344..18969
                     /gene="groL"
                     /locus_tag="VIF08_01875"
     CDS             17344..18969
                     /gene="groL"
                     /locus_tag="VIF08_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779552.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8134759.1"
                     /translation="MAKQLIFDETARRSLKRGVDRLADAVKVTLGPKGRNVVLDKKFG
                     APTITNDGVTIARDIELEDPFENMGAQLLKEVATKTDDVAGDGTTTAVVLGQALVTEG
                     LKNVTAGANPMIVKRGIDKAVEAIVAELKKNSRPVDNREQISAVAAISAADPEVGETI
                     AEVMDKVGKDGVITVEEGQSLGLEKEYTEGMQFDRGYISAYFVTNPDRMETVLENAAI
                     LITDKKISSIQELIPSLEKAVQLGKPLLIVAEDVDGEALATLVVNKLKGTISVLAVKA
                     PGFGDRRKEMLRDMAILTGATVISEELGRKLESVTPEDFGHARRVVATKDDTTLIEGA
                     GSPEEIKGRMTQIKAQVEETTSDYDREKLQERLAKLAGGVAVIKVGAPTEVELKEKKH
                     RIEDALSTTRAAIEEGLVAGGGVALLQAIPALDLVELVGDEAVGLGILRRALEAPVRQ
                     IADNAGAHGEVVVEKVKGLESGHGFDALKGEYGDMFAKGIVDAAKVTRSALQNAASIG
                     AMVLTTEALVTDLPEKHRAAAPAGAGYGGDMDY"
     gene            19519..21384
                     /locus_tag="VIF08_01880"
     CDS             19519..21384
                     /locus_tag="VIF08_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134760.1"
                     /translation="MPPIRPRAWILLPIVLMWSLGATGAQAQSPGASPDASPTTLDPL
                     VEDALAGALEDPAAIAAGEAAHRHGERAALGVAAIDPELPEIMDQLDAIAAEVLGGYL
                     AEPPFPTDPGATDGLGTPPGQGDRGRPLAMLQAQIGLNLTWGMASAAAESFIGGWRPV
                     PGTATLPGRPISTSGTFEGGGRFTSYTTTLSDALRVENGTIHLSYEFTMHSEVLDSAS
                     NATVAGIDDRGRVAISINPCPAAGGRVDTVVEAESDLTVRRPGGGSSSAREHATATAI
                     TTVTDGAEIGQTVTDLAARRTTTGSSSSDVGFTARFGADGRPTSSTQTQDDGSDADLL
                     AAAQGAAAAALGSVSRLVDAARTVWRARCVTVTPDPAGREVFAGSDTSISVTVHHAVE
                     DRDIEVPVRATLEGVESIDPNDEPDDAPADITYRAATQPRAEGKVTFKSTSNRGLAQR
                     TETYRTGPRLLVDVDGDVDASLGQAKSTFTVTGRDLKVTFGPGDPPTVRLEGRVRIRG
                     RTSDPTGCRSDYSGAIAVDPDASARLVMDAEVPRLAVLLLATDPAATVRVRASCPSGS
                     STSPFPAAALLGVWALARDPAVVDLPNGSTTVRVARSGPTPRQTWTISVRPASGD"
BASE COUNT         3256 a         7436 c         7492 g         3357 t
ORIGIN      
        1 cgacgcgcgg gctcgtcaac ggcgtcgtcc acggcgtggg cggctacggc aactgcgtcg
       61 gcgtcccgac aatcggcggg gagctgacgt tcgacccgac ctacaccggc aacccgctgg
      121 tcaacgtgat gtgcgtgggc gtgcttcccg aggaccgcat cgcccacgcc gcggcgccga
      181 cgcccggttc gctggcagtg ctctacggct cgacgaccgg ccgcgacggg atcggcggcg
      241 cgtccgtcct cgcgagtgcc aacttcgacg aagcgtcggc gcagatgcgg cccagcgtcc
      301 aggtgggcga cccattcgcc ggcaagctgc tcatcgagac gacgctcgag ctcgttgagc
      361 gtggcctgct cgaggggttg caggacctgg gcgcggccgg cctgacgtgc gcggcgtcgg
      421 agctcgccga ccgcggtggg accgggatgc gcgtcgacct cgcagcggtc ccgcggcgcg
      481 aggcgggcat ggcgcccttc gaggtgatga tcagcgagag ccaggagcgg atgctcgcca
      541 tcgtcacgcc ggcgcggctc gatgaggtgc tcgaggtgtg ccgtcgctgg gggctaccgg
      601 cggctgtggt gggcgaagtc acgggcgacg gcgatgtcgt tgtcgtggac gcgggccagg
      661 agatcgcgcg cgtgccggcg cgggcgctcg ccagcgaatc catcgagatc ccacggcttt
      721 catctccgcc ggagcgacgc cgctccgcgc ccgcgccggg cgaaacgccg ctggcgcacg
      781 atggcctgcc cgaacggggc atggaccccg cggccgtgct ggaagcactg ctgggtcatt
      841 ccaacctggg cagccgcgcg tgggtcacca cgcagtacga ccagaccgtt ggcgcggaca
      901 ccgtggctgg ctgcgagcgg gccgccggca tgctgcgcat caagggcacc aagcgcgcga
      961 tcgtggtcgc gaccgactcg cagccggccg tcgcgctgca cgacccggcg ctcgccgcga
     1021 gcctcgcggt cgccgagtgc gcgcgcaaca tcgccgtcac aggcgcgcga ccgctgggca
     1081 tcaccaactg cctcaacttc ggcgatcccg gggtgcccga cgcgtactgg cagctcgagc
     1141 agtcggttcg cggcctgcgc gacgcggcca ttgcgctggc gattccgatc accggcggca
     1201 acgtcagcct gtacaacgaa tcgccgctcg gacgcatcgc gcccaccgcg cagatcggcg
     1261 ttgtgggcgt gatcgatgac atcgccacgc tcgtcgcgcc cgcgttcaag agtgagggcg
     1321 acatcgtggt gttactgggc gaaggcgggc cgggcctcgt cggctccacg tacgagctca
     1381 tcgcgggggc cgcgccggat gaccggccgc cgtcgctcga gatgacccgg gagctggcgc
     1441 tgcacgaggt cctgcgccgc gccgccgccg cgcgcctcct cgaatcagcg cacgacgtga
     1501 gcggcggcgg cctcgctgtc gctatcgccg aagcggccat ctggggcgac ctgggcgccg
     1561 acctgcaggt ggccgtcgcc ttggcaccgg ccgtggtgct gttcggcgag agcccgagtc
     1621 gggcggtcgt aacgatcaaa ccggagcgct ggtcggcgct ggtcaaggtc gcatcaggcc
     1681 tgcgcgtcgc ctgccgcaag ctcgggtcgg tcggtggctc acgtctgcgt atcaggctcg
     1741 tcggtgttgg ggcgacgggc gcggctgagg aacgcggcgc tggtgtcgcc gatgagctcg
     1801 acgtcgcgct cactgatctg cgccgggcgt gggatcaggc gctgccgcgc gtcatgggcg
     1861 agggctagcc cggatgtgcg gggtggtggg cgtccacgcg ccgggtcacg aggcggcgcc
     1921 gcttgcgacg acggcgcttt tcgccctgca gcatcgcggc caggagtcgg cgggcatcgc
     1981 cgtcacggac ggccgcggtg tgatggtcta caaggacctg ggccacgtcg accaggtgct
     2041 gcaggagcac cgcctcgagt cgctgcgcgg cggcctggca atcgcccact gccgctactc
     2101 gacgaccggc tcaacgatct gggagaacag ccagccgact ctgcgcatgg gccctcggcg
     2161 caccgtcgcg gtgggccaca acggcaacct cgtcaacacg cgcgagctgc tcgcggcgct
     2221 gcccggtggc aaggcgcgtc tcgtcggcac gacggacaca gagctcctca ccgcgctgct
     2281 ggcgctcgag ccggctgagg atctggttga ggctctgctc gcgctcctgc cgcgggtgag
     2341 cggcgcgtac tcgctggtgg tgatggacga ggggcgggtc atcggggtgc gcgatccgta
     2401 cggcttccgg ccgctcgtgc tgggcacgct ggccatgccc gacggcgcga ccggctacgt
     2461 cctcgcgtca gaaacagcgg cgctcgatgt cctgggtgcc gagttcgtgc gcgatgtcga
     2521 gccgggcgag atggtcgtcc tgggcgagcc cggtgggcct cgctccgtgc ggttcgcgga
     2581 gggccgcgag aacctgtgcg tgttcgagct gatctacttc gcccggcccg actcgtacat
     2641 gctcggccgc aacctgtatg aagcacgccg gcgcatgggc gaggcgcttg cgcgcgaggc
     2701 gccggccgat gtggacctgg tgatgccggt ccccgacacg ggcgcaccgg ccgctgccgg
     2761 ctatgccgag gcgagcggca tcccataccg cgagggcatg gtccgcaacc gctacgccgg
     2821 gcgcacgttc atccagccgt caaacggcct ccgccagcgc ggagtgctgg tcaagctgtc
     2881 ggcgctgcgc gagcaggtcc gcgacaagcg cctcgtcgtg gtcgacgact cgatcgtgcg
     2941 cggcaacacc acgcgccaga tcgtcggcct gctgcgccgc gccggggcgc gcgaggtcca
     3001 cgtgcgcatc agcgccccgc ccatctacca cccgtgcttc tacggcatcg acacccagat
     3061 tgagaccgag ctgatcgcag cggccaaatc ggttgaggag atccgcgact tcatcggcgc
     3121 cgactcgctg tcttacctga gcatcggcgg cgtgctcgac gcgatcaacc tgccgcgtga
     3181 gcgattctgc ttcgcctgct tcgacggcca gtacccggtt cccgtgccct acgacccggg
     3241 caaccacaag ctggtgctcg agcgcgacga ggagcctgtg gccgctgcag tggcggtggt
     3301 cggccgatga cccgcactgc ctatgcccag gcaggcgtgg acatcgacgc gggcgagcgc
     3361 gcggtggcgt tgatgcgccg ccacgcgcaa tcgctgagcg gcgagttcgc ttcgatcgcg
     3421 gcggttcccc cggggatgcg cgagccgctc gttgtgagcg ccaccgatgg ggtagggacc
     3481 aagaccgaca tcgcgcgccg gctgggccgc tacgacacga tcggccagga tctcgtcgcg
     3541 atgtgtgccg atgacgtcgt gtgccacggg gcggcgccgt ccttcttcct ggattacgtc
     3601 gccgtcggac gcatcttgcc cgagcgtgtg gccgagatcg ttggcggcat cgcagctgca
     3661 tgcgcagaga tcggctgcga gctgttgggc ggggagacgg ccgagcatcc gggtttgatg
     3721 ggtcacgacg agttcgacct ggccgggttc tgcgtcggct tcgtcgagcg cgacgcggtg
     3781 atagatgggt ccgcggcgcg ggccggcgac gtagtgattg ggctcgcttc gtctggcctg
     3841 cattcgaacg gcttctctct gatccggcgc ctcgtcgatg acgggcgatt ggcgttgacc
     3901 gacgagctgc tcacacccac gcggctctac gcccgcgccg tgctcggcgt gggcggcgtc
     3961 cacggcatgg cgcacatcac cggcggcggg ctggcgcgca acctgccacg tggggttgga
     4021 ccagcacttg gggtgcgcgt cgatccgaca agctggcggc agccggcgat cttcgatacc
     4081 gtcgcccacg ccgtcggcat ggatggggca gagatgcgcg cgacgttcaa ctgcggcatc
     4141 ggcttcaccc tgatcgtgga gccagatgcg gcggccacgg tcgtcgggca cctcgaggct
     4201 cgcgggatcg aagcgtggcg tatcggacat gtgcgcccgg tcgacgagct cggcggcagc
     4261 cgttacgtag aggcgtgacc ggccgcatcg ccgtcggcgt gtcgggcgcc ggctcgaacc
     4321 tgcgcgcctt gcgtcgcgcg gagaagcgcg gactgctggg tggcacgatt ggcctggtat
     4381 tcgccgatcg gccgtgcccc gggctcgagt tcgccgccgc ggagggcatt gccacggcgt
     4441 tggcggcgcc ttccgactac cccgaccggg cgagctggga tgcggcactc gcgcagacgc
     4501 tcatcggcgc cagcgtggat tgggtggtgt tggccgggct gatgcgggtg ctcggtgatc
     4561 gcactcttgg cgcgttcccc gagcggattc tcaacatcca tcccacgctg ctgccctcat
     4621 acccgggcgc ccatccggtg cgcgacacgc ttgccggcgg cgcgcggatc accggcgtga
     4681 cgattcacat cgtggactcg tcgctcgacg gcgggccgat cgtggcccag cagccgctgc
     4741 cgcttcccca aggcgaggcc ctgaccgatc tgctgccgcg catccgtgcg ctcgaacacc
     4801 agctgctgcc gcgcgtcgtg gctctcgcac tagccggtgg gatcagcatc gacgacggtc
     4861 gcgtcctcat cgacgaggcg atcgccggtg agctgccgcg gccgcgacgg gtgctgctct
     4921 cggtctcgga caagagcggt ctcgtcgagc tgggccgttc cctggcggac ctcggtttcg
     4981 accttgtttc aactggcggc acggcgcgcg cgctgcggga cgccggcgtg gacgtgatcg
     5041 atgtggccag cgtcaccgga tttccggaga tgctcgacgg gcgcgtcaag accttgcacc
     5101 cgcgcgttgc tgcgggcgtc ctggccgatg tgcgctcagc cgagcaccgc cgccagctgg
     5161 ccgctgcggc catcgatcca ttcgagctcg tggtcgtcaa cctgtatcgc ttcgcggaag
     5221 cggcagcgcg gccgaacatc gatgacgacg gcctgatcga ggagatcgac atcggcgggc
     5281 cgacgctggt gcgggcgtcg gcaaagaacc atgccaacgt cggcatcgtc accgaccccg
     5341 cgcagtacga ggacgtcgtg gccgagctgc gcgaccgtgg ccggctgtca gacgagctgc
     5401 ggcgcgagct ggccgcggcc gcgttccggc tgactgccac ctatgacggg ctgatcgcgg
     5461 acaccttgtc gcgccgttgg gggacgggca ccgagcagcc agggcgcatg tcgctcgacc
     5521 tggagcgcgc cgaggagctg cgctatggcg agaacccgca tcagtccgcg gcgctctacc
     5581 tggcgcgcgg tgtcgacctg gcgccgggtg ccttcgcgaa cgggccgctg cttcagcagg
     5641 gcaagccgct ctcctacaac aacatcctcg acacggccgc tgcgactggt ctggcaagag
     5701 acctgcgcgg cgcggcctgc gtgatcgtca agcacgccaa tccgtgcggc gtcgcggagg
     5761 cggacgatct cgcggctgca tgggagggcg cgctggccgg ggatccagtg agcgcgttcg
     5821 gaggcgtcgt ggcgctgacc ggggccgtcg acgcggcgct cgccgagcag cttacgtcga
     5881 tcttcctgga ggtcgtggcc gccccggagg tgacgaggga agcgcgcgag atcctggctc
     5941 gcaagcccaa cctgcgcctg ctcctggatc cgggactcgg cgcgccgccg ctccccggca
     6001 tggagctgcg cagcgcaggg ggcgcgctgc tcatgaccca ggccgacgtc gcgcctgatg
     6061 acccacagag ctggacggcc gcgaccaggc gcgcgccaac ggacgaagaa cgcgccgcgc
     6121 tcgatctggc gtggcgcgtc tgccgccacg tcaaatcgaa cgcgatcgtg ctggtcaagg
     6181 gccgcgcggt ggtcggcgtg ggcgccggcc agatgagccg cgtcgacagc gcccgcctgg
     6241 cggtcgcgaa ggccggaccg gagcgcgccg cgggcgcggt ctgcgcgagc gacgccttct
     6301 acccgttccc cgatgcgctc gaggtctgcg cgggggccgg cgtgacggcg ttcgtccagc
     6361 ccggcggctc ccagcgtgat gcggaggtga tcgcggccgc ggacgccgcc ggcgcggcga
     6421 tgctgatgac cggcacgcgc catttccgcc attaaggggg gcttatcgcc cccgggctta
     6481 gaatcggtgc atggacgaaa acatgaaccc gaacaacgag cccatggccg atgccgagcg
     6541 gccccgcgcc ggccgcgaga tgctggttca gctccagcag atgatcgaca cgctggccac
     6601 ccaggccgcg ccggtcatgc gcgaggttgc cgcaaaggcg gcagagctgg ccgcgattgc
     6661 cggcgagaag gccggtccgc tggcccacag ggccgccggc atgaccgaga gtgcaggcca
     6721 gaagatcgcc gcgcgatcaa aggaggtcgc ggccgacctg cgccgccccc gtgacacggc
     6781 cgacccactg gccgacccaa tggccgacgc ttctcccgac gcaccccccg acgcagagcg
     6841 ctccgagcac gaatcggtcg gcgagtagga agccccagcg ccaatagaaa aagccgggcc
     6901 tcgcggcccg gcttttgtct tgctgaaagg agcctgcgct ctagcgctgc cccttcttgc
     6961 ggttctcggc ttccttggcg cgctggcgat cgcggatgtc gcggtccagc tcgctgcggc
     7021 gctcgatgtt gcgcgtggtg tagtagtcgt gcgtcgcgaa gatgttgtgg ccttcgacgt
     7081 cggactcctg cccgggcttc tcggcaacct tcttcttcgt ttccttcgcc ttggtcattg
     7141 aacctgggct ccttcctgca tgtgtatggt gatctgcata cctagcgcac tggcgtcagc
     7201 atcggccttc gcacgcggcg ggtcaatgcg cgagccgaca cggcgcctgt aaagtggccg
     7261 acaccgggcg cgggcccgtc agagcaagct gagggacgat gggagtaatc gccgcctacc
     7321 ggcgcgtgct cggcaacggg gcgttgtcac ggctgctttt cggcgagttc gtgtcgtcca
     7381 ttggagactg gctgtacctc gtcgcgctgc tggtgcttgt ctgggacgcc accggcggcg
     7441 acacacttgc cctggggctg atcggcgccg cgcgcatcct gccgtatgtg ctcctgtcga
     7501 ttccggcggg catcgtggcc gaccgcttcg accggcgcaa gatcctgctc ttcaccgaca
     7561 tcgtgcgtgg cctgctcatg ctcgtgatcg cggccgcggt ctacctggag ctgtcgatct
     7621 ggatcatcgt ggcgctggcc atcacggcga cgtgcttctc ggccttcttc gggccggcca
     7681 tcggcgcgta cctgccttcg cttgtgcgcg acgagtcaga gctgggcccg gccaacagcg
     7741 cgtggtcgag cctggtcaac ctggcgttct tcatcgggcc cgcggtcgcg gccctattgc
     7801 tgagcatcgg ctcgctggtc gcggcgttcg tcctcaactc gctcaccttt gcgatcgtcg
     7861 cgattgtgct gctgggcctg ccctcgaagc aaccgggggt aaagactgag gcggcggccg
     7921 atgcagcgga gccaaccaag gcccaaccgc tgatgaacgc gctccgcccg atctcgcggc
     7981 cgctgctcgg cctgacggtg ctcaacatgg tcgtcggttt cgtatggggc ggcatgggcg
     8041 tcgtgacggt catcctgtcg gtcgaggtct tccacatcga cgaggcgacc ggtaccggcc
     8101 tgctcaactc ggccattggc gttggtgggg tcgtaggcgc gctcattgcc ggggcgctgg
     8161 tgctgcgccg ccgccagggg ccgccgctgg tcgttggcgc agcggccctg ggcgtgggcc
     8221 tggccctgct cggccaggta ccggacttcg cgctggccct ggcggcgctc ggctttgcgt
     8281 cggctggcgc gctgctggtc gagatcgtca tgaccacgct gctccagcgc atcgtgcctg
     8341 acgcggtgcg cgggcgagcg ctgggcctga tcgacacggt ctacgtcttg ttctacgcgg
     8401 ccggcgcgtt tgccatcccg atcatcgcct ccggccaggc cggcctcgtg ctcctcgtct
     8461 ccggcatcgc gattgccgtc tccggtgtgg tcggcctggt gttgctcggc gcctacgcag
     8521 tccagcagcc gcccgacgac ctgcgccgcc ggctgggtga cgtggcgctc ttcgctggtt
     8581 tgccgccggg ccgcatcgag accgccatgc gccacgccac gctgcgcgac gtgaaggccg
     8641 ggacgacgat catccgccag ggtgacccgg ccgacttctt ctacgtcatc ggcaacggcc
     8701 gggtcgaggt cgcccagacg gatgcgaacg gaacgagccg cgtgctgcgc cagatgagtg
     8761 gtggcgagtt cttcggcgag attggcctgc tctcgcgcgt gccacggacc gcgaccgtcg
     8821 tggcgctgag cgattgcacg ctcgtcgcgc tcgagggcgc ggccttcctg gagctggtcg
     8881 aatcgggacc gggcttgacg tacaagctgc ttgacgttca ccgtggcacc gtgagcgcca
     8941 ccggggaggt ctcgtaggct tggacgacac cttcgcggtt gaggtcctgg cgcgctgccc
     9001 gctcttcgcg cacgggtcgc gttcgatcct cgagtccctc gcaggcacgc tccgccggcg
     9061 ccgcttccgg cgcggcgagg tgatcatgca ccagggcgat cccggcgact cactccacat
     9121 cgtggccgca ggcgcggtca agatcctgct gccgtcggcc gagggcgagg aggcgatcat
     9181 cgccaccctg cggccaggtg acttcttcgg cgagctcgcg cttctcgacg gggcgccgcg
     9241 ctccgcaacg gcaactgcgc tcgaggcctc ggacacgctg gtcctaccgc gctcggtgtt
     9301 catggagctg ctcgacacgc tgcccggact gcgcgatgcc cttctggcag ggttggcgca
     9361 cgagctgcgg cgcctgaccg gccacgtgga ggagctgcac ttcctcgatc tggccggtcg
     9421 actggcgatg cgcctgagcc gcctggcgtt ggaggccgca ccagacgcgc atggcgagat
     9481 tcgcctcgaa tggccctaca cccagtccga cctcgcggcg atgatcggcg gtacccgcca
     9541 gagcgtgaat cggcttctct cggagctggt cgaggacggt ctgatcagaa tcgagcccga
     9601 cgcgctggta atcggcaatc tcgacgagct ggcccgccgc gccgaacgct gattaggggg
     9661 gaactcggga ctagacctgc gctcgcccga cattcatgct gtcgggtatt cgacagtcca
     9721 cctgtccgct cgtcggttgc cgcggcggcc gccgtgcccg aatctgaaaa acagccgaac
     9781 aggacggcag ccaaccatag ggaggtttcc atcaatgcct agaccaatca ggccagccaa
     9841 gatgttccgc gccgagggcg tttcgccggt cgacgaggat accgaaggcc accgcatggg
     9901 catcaacagc gctaaaggtg cgctcggcgc caagggtgcg ctcggtgcca agggtgcgct
     9961 cggtgccaag ggtgcgctcg gtgccaaggg tgcgctcggt gccggcggcg acgacgagga
    10021 cacagagggc cacaggctca gcaaggacta gcttcgtcct ccctttccgg gatgaccgat
    10081 cccggcctga ccgccgcgct ggaagcctta cggcaggcag cgcggcggtc ttctgttgcc
    10141 cgccgcctcg acgctgacac ggagcaggcg ctcctccaga cgatcgtcga ggcgacggtc
    10201 atgctcttcg aggccgaggc gtcatcaatc gcgctgttcg aacgcgaccc cgaccgcctc
    10261 gagttcaggg tcgccgcggg cgcccaggga gcgggtgcga taggcctttc cgtccccccg
    10321 tcgaagggca tcgtcggctt cgtcttctcg accggccagc cgctggcgct gagcgatgtc
    10381 ctggccgatc ctcgtttcga caagaccgtc gcggagaaga ccggctatgt gccgcgctct
    10441 atcgccgctg tgccgctggt cgatgccgag acgacggtcg gggtcctgca ggtcctcgac
    10501 aagcacacct cgccgacctt ctcgctgcgc gacatggagc tgctcgcggt cttcgcccgc
    10561 caggcagcgg cggcgatccg cgccgcgcgc gtgcagcgcg acagccaacg gttgctgcgc
    10621 gcggcgctgg ctggtgtcgg tggcgcggac gtcagcgacg agcagctcga catcctcatc
    10681 tcagccgcga ccaccgagct cgacactgac cccgacacgc ccttctggcg cctggtcgac
    10741 cgcgttgcca ggatgcgcga cctgtcggac cgcgagctgc gcatagtgag cgacatcctc
    10801 gacgtcctcg ccacgcacgc cggtcgcggc cgccgcagcg tctagtcgga cagcggtgac
    10861 tgaactcgat ctcccagcgt ggagcgagcc ctttacggac gacgagcggc acaacctggt
    10921 tcgcgccgca acgctcaagc agctcaatcg caaatgggca tggggcggcg ccaccggcaa
    10981 gggggtgaag gtcgcgatcc tcgactccgg cgtggaaggc agccatcctg tagtgcaggg
    11041 ccggctggtc gagtcggtcg cggtcgagat cgtcgacgag gaggcgaccg tagtgcccga
    11101 tgagccggtt gacctgtacg gccacggcac tgcctgcgcg ggcatcatcg tgggcctggc
    11161 gccggaggcc gagatctact ccgtgcgggt gctcggatct gacctcaagg gcaaaggagt
    11221 ggccttcctg cgcgggctcg agtacgccgt cgagaagggc gtccaggtga tgagcctctc
    11281 gctttcgtcc aagagcgaac gcctcttccc gttcttccac gaggtcgtcg acgaggcgta
    11341 cttcaggcac gtatgccttg tcagcgcggt gaacaatgtg cccggcctct catatccctc
    11401 caccttctcg agcgtcttct ccgtggccgc gcacgcaatc ccggaacctg agacgttctt
    11461 ctacaaccct tcgccgccgg tcgagtgggg cgcctgggga gtcgacgtcc cgatcggctg
    11521 gaaggacggt gcgactacgg tcgcgaccgg caacagcttc gccgcccccc acgtcgccgg
    11581 cttcgtggcg cggatcctct ccaagcatcc tggccttacg ccattcgagg tcaaggccgt
    11641 cctcgccgcg atcgccaacg acccagggtg atggccaacg acccaaactg aagcgccggc
    11701 cctggtgatg ggaccggcgc ttctttgagg aggagttcga ttgggcgcta gatcagcgcg
    11761 acgactccga ggacggtgac cacgcttgcg gccacggcga ccaggtgctg gcggaagttg
    11821 ccaaccacgg tctcgactgc gtgagtgccg tggtgcgcct gcgccgcgcg agctgcgtgc
    11881 tccttgcggc gaagctcggc ctcgtgcagg gcttcctggt ggtagaagct gggattgaag
    11941 tccagcgctc cggggatccg tgacatctga gttctccttt gtcggttggg ttccggctgc
    12001 ttcatctctt gtctgttgtt gatgggcaaa tgctcgcttc gcgcaacgct ctgcaccatc
    12061 aaagggacga cgaacgggcg gtcaacattt cgaccgatcg tccgtcgctg actcacgccc
    12121 agatctgtcg gccagcggac acgaaggggg aaccgctacg atcgccgccc catgaccgcc
    12181 gacggcgcgc gcgagcgctt ctacctcacg accgcgatct tctatcccag cgcgcggccg
    12241 gcgctgcact cgctcttcga ggcgatcggc gccgacgcga tcgtgcgcta ccagcgcatg
    12301 ctgggtaagg acgtgcgctt cctgaccggc atggacgagc acagcgcgaa cgtcgagcgc
    12361 aaggcgaccg aggaaggcgt gacgccgcgc gacctcgttg acccgtgggc cgccacgtgg
    12421 cgtgcggcgt tcgacaagtt cggcatcagc tacgaccgat tcatcaggac caccgatgcc
    12481 gaccacgccc gggcgtcaac cgagatggtc acgcgcgcca tggcctccgg tgacatctac
    12541 aaaggcacct attccggttg gtactgcccg ggcgacaacg agttcaagac cgaccaacag
    12601 ctcgtcgatg ggcgctgccc cgatcacccc agcctcgagc tgcagtggct cgaagaggag
    12661 aactggttct tccgcctctc ggcttaccag gaccggctgg agcagctgta ccgcgacaac
    12721 ccttcgttct gcgagccgga gcactaccgc aacgaggttc tgggctggct gaaggacggg
    12781 ctgcgcgact tctccatcag ccgcgccggc ggccaatggg gcatcccgtt cccgaccgac
    12841 cccgatcacc gcatctacgt ctggttcgac gcgctcacga actacatcac cggcgccggc
    12901 tttccggacg acctcgacgc gttcgccagg tggtggccgg tcgacgtgca cgtcatcggc
    12961 aagaacatca cacgcttcca ttgcctgtac tggcccgcga tgttgatgag cgccggcatc
    13021 gcgctgccca agcaggtctt cgcccatggc ttcatgctcg accgcggcgg cgcgaagatg
    13081 agcaagaccc agggcaacgt gctcgatccc gacgcgatgg ccgagctgat gagcatcgat
    13141 ggcgtccgtt acgccgtgct gcgcgaggtg ccattcgatc gcgacgcaga tgtgtcgtac
    13201 gactcgttcg tgcgccgcta caacgccgat ctcgcgaacg attttggcaa cctgctcaac
    13261 cggaccctca cgatgacgag ccgcttcctg gacggcgagc ggccggcgcc cgcgacaacg
    13321 ggcgagcttg gctcggcttg ggcgaccgcc tgggggtcgt acaccggaca gatggactcg
    13381 tacctgctgc acaaggcgct cgaagccctg tgggacttcg tcggtcacgc caatcgcttc
    13441 gtcgacgacg agcagccctg ggcgctcaac aaatctgcca aggcgggcga cgcggaggcg
    13501 gccgatcgac tgcgctcaac gatggccgac ctgttggagg cctgccgggt cgttgcgctc
    13561 gccggcgcgc ccttcatgcc gggcgcggca cagagtgcgc acagccagct gggcctcgat
    13621 tacacgtatg cggccaatgg cagcgacggc ccaccgctgg ctgagcaggc cacctggggt
    13681 gcgctgggcg ggggagggaa agtcggagcg caggacatcc tgttcccgcg tgtcgaaatc
    13741 gaggcacccg gttcgtagta gtcgtgaagg aggtgctgaa ttggcacacg gacaactgac
    13801 acacctcgag atcccggccg acgacctgcc gcgcgcgcgg cgcttctaca gcgagctgct
    13861 gggctgggag atcggtgaca tgccggagtt cgcggactat ccgctcttca gcttcggcgc
    13921 gatcgagcgg gcgggcggcg ccctggggct gcgcggcaag accgtcggca atcaactgcg
    13981 ggtgtacttc gacgtcgact cgatcgacca agtgctgccc aaagtcgcca ggctgggcgg
    14041 caaggtggtc gagcccaaga ctgagatccc gaacatgggc tggtacgcgg tgatcgacga
    14101 caccgagggc aaccaggtcg cgctttacca gggcatgccg cagcctgcaa tgtgaggctg
    14161 atcgactcgc actgtcatct ccaggcgggg gcgtttgcga ccgatgtcgc cgacgtcctc
    14221 gcttctgcgc gcgaggcagg agtggaacgg gtcctggcgc cgggctacga cctcgagtcg
    14281 tcgcgcgcat cgatagagct tgcccgcacg cttggcgtag acgcatcagt cggcatccat
    14341 ccgcacgtcg cggacgggtc tgaggccgac tggccgaaga tcgcggcgct cgccgccgac
    14401 ccattggtcg tcgcgattgg cgagaccggc ctcgactacg accgcggctt ctcgccgcgc
    14461 gaggcccaga tcgccaacct ccggcgccac ctgcggctcg ccgcggaaac caacaagccg
    14521 gcaatcctgc actgccgctc caagcccggc cagcgcgacg cccaggacga cctgttgcgc
    14581 gagctggccg acgccagcgc gccgcctgcg ctgctgcact ccttctccgg cccgctcgac
    14641 tacgcggagc gtgcaatcga catgggcgtc gcgatgtcct tttccggcct cgtcttcagg
    14701 acgggggagg aggccagcgc agaggtagcg cggataaccg cggccgatcg gctgctggtc
    14761 gagacggact cgccgtacct ttcaccgccc ggcgccccca ggcggcgcaa cgagccgcgt
    14821 ttcgtcgaga tcaccgcacg ctggctggcg gaacagcgcg gcgacgaccc cgaagagctc
    14881 ggcgcccgcc tggtcgcgaa ctacgaccgg gtcttcagcc gcccacccag ctagacggat
    14941 cggtctcgag acgcgcccgg aacagctcgc gctcgtcacc ccataactgg tacagccagg
    15001 ccgagtgttc gctccagccg cggcctatca ccttggcgcc ggcctcggct gaccagagga
    15061 actcggcccg ataacgacgc ggcaccggta gcccgtgccg gatgcgctcc aggcccagga
    15121 actgctcgtc catgtagtcg agataggccg cgttgttgac atgcgccatg gggtcgagct
    15181 cgctgcgccg cgggcgaaac tcacgcgcgc tggccgactc cggtgcagtc ggagcctcca
    15241 tgcgcatggg tgtgaactct ccgaaatcgg agccgaagac gtccaggatc tcctgcggcg
    15301 ggcggaccgg ccgaccctcc tcggtgatca ggacccagtc agtgatggcc acggcgatga
    15361 tgcgttcttc gccggccggt ttgaactcac tccggcggcg ggcccagacg cggcgaaagc
    15421 cggtcagctc ggtcgagacg tcgaggtcgt ggctgtacgg cacctgggcg agcaggtcca
    15481 gctcgatcgc gcgcgccagc cagagcaggc ggcgttcgcg gtaccagtcg cggtcgaagc
    15541 ccgcgctctc cgaatggacc cacgcgaggt cctgggcgaa acgcagaaag cccgacgcgc
    15601 gcaggtaacc gtccggaccg gcctcgtcga aacggacccg atagggccgt tcgatccggc
    15661 gctcgaccgt cacgccggcg acttctcgcg catgaattcg agcacccgcg ggtcgatcca
    15721 gccacctgcc gccgcgcgct cctcgaggtt ctcgatggac atccactcgt cgagcgcgcc
    15781 ggtccacgac gcgtcccagt tcgccggcag atctcgtggc tcgccggttc gcgtgagccc
    15841 tgagcgctcg actatctcgg ccaggctgtt cgacgtgtcg agcttgtcgg gtgcgtagcc
    15901 gactgccgtc aggcgttgcc taagctcgtt ggccaggctt tcgtcgatcg gcaccaggtc
    15961 ggtcgcggcc gggcgatcga gatagaggtg attcagatcg agcagccggg cgacctcgcg
    16021 catcgggtcg gggtgatcat cgacgcgcag atcgatccag cggtcggagc cgccaccgta
    16081 gccgccccct tcgcggacca cgagcagcgc ggccgattcg cggccacgcc gatcgccacc
    16141 ctggtcgtcc gcggcctcca gcccggccag tagtcgctcc gggaacgcgg ccgtgctcgc
    16201 gaggaacgtc tcgatcagcg cgtccacgac gctcggcccc gccaggagat tgccctgggc
    16261 cgcgacgccg tccgccgttc ggccgccggc ccaggtgatg cagccgctgc cggtgtaggt
    16321 cgcggagccg cccgccgcgt ccacgatgcc ggcctggcgg tggtcgcgcc cttcgtcgtt
    16381 gctcgtcagc tcggccagga cgtcggttgc gctcttgccg gcggcgagga gcgccaggcc
    16441 gtccgcgcca taacgaacgt tggccagtgc ctgggtcgcg acagcgccca cgccggcgcg
    16501 ggcatggggc acgactgcgc ccacggccag gaattttgac gcgacggcaa tgcccagatc
    16561 gcccgtcgct gggtcgcgcg ccacgatcga gaacgtcatg ctgccagtac ctccgcgatg
    16621 cttgtcgtgc gttttgcttt cgtggtgggc gttgctctgt cgaaggttat gcgtgtggtg
    16681 caacggcacc ctcgtcgggc ggcccgcgac tcatgcggcc ggctatgcgc tgggtgcacg
    16741 actaagcgcc agattcaggc ccgcggtcat cgttcgccgg acgagtcgtg cgcagggtgc
    16801 acgaaccacg caggcgcagg gcgtcgcgct tgacacctca cccggcactg cgtaaccttc
    16861 tagcactctc agacagagag tgctaaatgc acaaagatcg gccatgcgcc gtgacccgcc
    16921 ggcccaaatc aaggcggacg cgcaccggcc atcgcacagg accaccagga gggtaacgcg
    16981 ttggcgactg ccactgcgac gaagcttcgg ccgctcggcg accgtgtcgt cattcagccc
    17041 actccgcgcg aggagatgac caagtcgggc atcgttctgc ctgatacggc caaggagaag
    17101 ccgcaggagg gcaagatcct cgccgccggc cccggccgct tgaccgacga gggcaagcgc
    17161 gagccgatgg acgtcaagga aggcgacgtc gtgctctacg caaagtacgc cgggaccgag
    17221 ttcaaggtcg agggcgagga cctgctcatc gtcagccaga aggacatcct cgcaatcgtc
    17281 aacagctagc cgggcgccca cgcgcaacgc ggcttttctg tttccaggag gtaactctca
    17341 cccgtggcca aacaactcat ttttgacgag accgctcgtc gctcgctgaa gcgcggcgtc
    17401 gaccggctcg cggacgcagt gaaggtgacg ctcggtccca agggccgcaa cgtcgtcctc
    17461 gacaagaagt tcggggcgcc cacgatcacc aacgatggtg tcaccatcgc gcgcgacatc
    17521 gagctcgagg acccgttcga gaacatgggc gcgcaactcc tcaaggaagt cgcgaccaag
    17581 accgacgacg ttgcgggcga cggcaccacg accgcggtcg tgctcggcca ggcgctcgtt
    17641 accgagggcc tcaagaacgt caccgccggc gccaacccga tgatcgtcaa gcgcggcatc
    17701 gacaaggccg tcgaggccat cgtcgcggag ctcaagaaga actcacggcc ggtcgacaac
    17761 cgcgagcaga tcagcgccgt cgcggcgatc agcgccgccg atccggaggt cggcgagacc
    17821 atcgcggagg tgatggacaa ggtcggcaag gacggcgtca ttaccgtcga ggagggccag
    17881 tcgctcggcc tcgagaagga atacacggaa ggcatgcagt tcgaccgggg ctacatctcg
    17941 gcctacttcg tgaccaaccc cgaccggatg gagaccgttc tcgagaacgc cgccatcctc
    18001 atcaccgaca agaagatctc cagcatccag gagctcatcc cgtccctcga gaaggccgtc
    18061 cagctgggca agcccctgct gatcgtcgcc gaggatgtcg atggcgaggc actcgcgacg
    18121 ctggtcgtca acaagctgaa gggcactatc tccgtgctcg ccgtcaaggc ccccggcttt
    18181 ggcgaccggc gcaaggagat gctgcgtgac atggccatcc ttacgggtgc caccgtcatc
    18241 agcgaggagc tcgggcgcaa gctcgaatcc gtcacgccgg aggacttcgg gcatgcccgc
    18301 cgggtcgtgg cgaccaagga cgacacgacg ctcatcgagg gcgccggctc tcccgaggag
    18361 atcaagggtc gcatgaccca gatcaaggcg caggtcgagg agaccacgtc cgactacgac
    18421 cgcgagaagc tccaggagcg cctcgccaag ctggccggtg gcgtcgccgt catcaaggtc
    18481 ggcgcgccaa ccgaggtcga gctcaaggag aagaagcacc gcatcgagga cgcgctctcg
    18541 accacgcgcg ccgcgatcga ggagggcctt gtggccggcg ggggcgtcgc gctcctgcag
    18601 gccatcccgg ccctcgatct cgtcgagctc gtgggcgacg aggcggtcgg cctcgggatc
    18661 ctgcgccgcg ccctcgaggc gccggtgcgc cagatcgccg acaacgccgg tgcccacggc
    18721 gaggtcgtgg tcgagaaggt caagggcctc gagtcagggc atggcttcga tgccctcaag
    18781 ggggagtacg gcgacatgtt cgcgaagggc atcgtggacg ccgccaaggt cacgcggtcg
    18841 gcgctccaga acgcggcctc gatcggggcc atggtgctca cgacagaagc gctcgtcacc
    18901 gacctgcccg agaagcaccg ggcggccgcc ccggccggcg ccggctatgg cggagacatg
    18961 gactactagg ccgatcgctc gtcacaacag ccacggggcc cgagccgcca ggctcgggct
    19021 ccgcggacag gagagcagca ccatgactga cgagctcggc gggcgccagc ctccacggac
    19081 gcctgcggcc cgaggccgcc ggttcaagcc gcatgcggcc gaactcacgg acggcagcag
    19141 gctcgttctg ggtgtcgatg gttcgatcga ccacgtcgat gaccaaggct ccaggatgca
    19201 ccgctggaag cctggcgatc cagggtggtc ggaccaggcg atccggttcg ggctgcaccc
    19261 ccaggcgccc accgtcgcgc cgcacggtcg ccgcgtccag gaaagcaagc cacctcgcgt
    19321 gtagcgcgct tcacagcaag agatccaggc ctggccgcca tgggacccgc gaccagcagc
    19381 cccgttcggc gctcgcggaa cgtgattgcg ccctagtgac aagcgcttcg ttctcctgga
    19441 ccgtccgggg gatcgggcgt aggctcatcg tcgtctcccg gccgcgggat cggcccggag
    19501 cgaccgaggt gctgcgcgat gcccccgatc cggccccgag cgtggatcct gctgccgatc
    19561 gtcctgatgt ggtcgctggg ggcgacgggc gcccaggccc agtcgccggg cgcctcgcca
    19621 gatgcgtcac cgacgacgct ggaccccctg gtcgaggatg cgctcgccgg agcgctcgag
    19681 gatcccgcgg cgatcgcggc gggtgaggcc gcgcacaggc acggggagcg ggcggcgctc
    19741 ggtgtggcgg ccatcgaccc ggagctaccg gaaatcatgg accagctcga cgcgatcgcc
    19801 gcggaggtgc tcggcggcta cctcgcggag ccgccgttcc cgaccgaccc gggcgccacg
    19861 gatggcctgg gtacgccccc ggggcagggc gatcgcggcc gtcccctggc catgctccag
    19921 gcccagatcg gcctcaacct cacctggggc atggcgtccg cagcggccga gtcgttcatc
    19981 ggtggctggc gccccgtccc gggcaccgcg acgcttcccg gccgcccgat ctccacatcc
    20041 ggcacgttcg aggggggcgg tcggttcacg tcctacacga cgacgctgag cgacgcgctg
    20101 cgcgtcgaga acggcacgat ccacctcagc tacgagttca cgatgcactc ggaggtgctg
    20161 gacagcgcct cgaacgcgac ggtcgccggc atcgacgatc gggggcgcgt cgcgatctcc
    20221 atcaacccct gcccggccgc gggcgggcgc gtggacacgg tcgtcgaggc ggagagtgac
    20281 ctcaccgtcc gccgccctgg cggcggttcc tcttcggcgc gtgagcacgc cacggcgacg
    20341 gccatcacca ccgtgaccga cggggccgaa atcgggcaga ccgtcaccga cctggcggcc
    20401 cggcggacga ccaccggatc gtcgtcgtca gacgtgggct tcacggcgcg cttcggggcc
    20461 gacggtcggc ccacctcgtc gacccagacc caggacgacg gcagcgacgc cgatctgctg
    20521 gcagcggccc agggtgccgc cgccgcagcg ctgggaagcg tgagccgcct ggtcgacgcg
    20581 gcgcgaaccg tgtggcgagc ccggtgcgtg accgtcaccc cggacccggc cgggcgcgag
    20641 gtgttcgcgg ggtcggacac ctcgatcagc gtgaccgtcc atcacgccgt ggaggaccgt
    20701 gacatcgagg tgccggtgcg agcgaccctc gagggagtcg agtcgatcga tccgaacgac
    20761 gagcctgacg atgcgccggc cgacatcacc tatcgtgcgg cgacccagcc ccgcgcggag
    20821 ggcaaggtga ccttcaagtc cacgtccaac cggggcctgg cgcagcgcac cgagacctac
    20881 cgcaccgggc cccggctgct cgtcgacgtc gacggcgacg tcgatgcgtc gctgggccag
    20941 gcgaagagca cgttcacggt gaccggccgg gacctcaagg tcaccttcgg tccgggcgac
    21001 ccaccgacgg tccgtctcga gggcagagtc cgcatccgcg gccggaccag cgacccgacc
    21061 gggtgtcgct ccgactactc gggcgccatc gcggtcgatc cggacgccag cgcgcgcttg
    21121 gtcatggacg ccgaggtccc ccggctggcg gtcctgctgc tggcaaccga tccggccgcg
    21181 accgtgcgtg tccgagcaag ctgcccctcg ggttccagca cctcgccgtt cccggccgcc
    21241 gccctgctgg gcgtgtgggc gcttgcccgc gacccggccg tggtcgacct cccgaatggc
    21301 tccaccacgg tccgagtcgc acgcagcggc ccgacgccca ggcaaacctg gacgatcagc
    21361 gtccgacctg cgagcggcga ctgatcggtc gctcgcgctc accgcccttc agctcctcgg
    21421 ccactcgtcg atcgtcttga cctcgagcac ctacgggcac gtgctggagc agcggcagcg
    21481 acaggtggcg ctagcgatgg acgcggtgct gggcgggcga cagggacgcg gccgcctcgg
    21541 a
//
LOCUS       DATBRX010000053         7527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2808609, whole genome shotgun sequence.
ACCESSION   DATBRX010000053 DATBRX010000000
VERSION     DATBRX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808609"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..829)
                     /locus_tag="VIF08_01885"
     CDS             complement(164..829)
                     /locus_tag="VIF08_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY8134761.1"
                     /translation="MPTNLDAFLADPQVTALIRLALAGLVAGLIGLERELAGKAAGIR
                     TYGLVGLGAAMFTVTAIYGFGSLDYASRIVGGVITGIGFLGAGAILHTKVHVVGLTTA
                     AGLWVAAGVGLAIGSGLYLLGIGAGLLMFVLLQFAGPEEFIRKRRKRMGLDSQDPEND
                     FVAEESASQDDDEPLRRTLRPLNAPEPQAPMSAWPGQIPAPPANGPWSTGNQQPPRED
                     GRG"
     gene            912..1703
                     /locus_tag="VIF08_01890"
     CDS             912..1703
                     /locus_tag="VIF08_01890"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEY8134762.1"
                     /translation="MPVADETLPATDARLADPALDLSGLARVWAAYGARRAMTAEQMV
                     GADRKAQRLGVAGRDLMEQAGTAVAVAARALVNSTDRPSSGHVLILAGPGNNGGDAFV
                     AARHLAEAGIRSYVVLVSAATKPGTPDAAANWARLEGVGEVQRMHATSGREVAILLNG
                     IERAALVIDGLLGTGVRGALREPVAQAVELCLAARRAGVPILSIDTPTALDLTSGEPS
                     EPNVQANLTVTFHRPKQGLQTRTGRALAGGVLVAPIGIPVEADHD"
     gene            1696..1932
                     /locus_tag="VIF08_01895"
     CDS             1696..1932
                     /locus_tag="VIF08_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134763.1"
                     /translation="MTDPASNPGPDSPPGARARDLLLALEVAGLIVVGVLVFAWVTDQ
                     VPVLGEALLFEPLVIIALVAVTIVILARALWPRR"
     gene            complement(1929..2558)
                     /gene="plsY"
                     /locus_tag="VIF08_01900"
     CDS             complement(1929..2558)
                     /gene="plsY"
                     /locus_tag="VIF08_01900"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEY8134764.1"
                     /translation="MLPQIALDGLFIAIGYLGGSLPMGVIVARLTGARDPRTVGSGRT
                     GGTNALRAMGAWRAGLVATLDVLKGFVPVAAAALLGASDLGVALTGVAAVVGAWASVW
                     LRFHGGRGVATGIGATVWMAPLAIPLAVPFFFGPILLTRYVSLGSLIGTLGGTIIIGI
                     LVLLGYNVWPHFLFAAIGTAIVWVAHHDNIQRLLRGEERRFDPVARGDG"
     gene            complement(2585..3313)
                     /locus_tag="VIF08_01905"
     CDS             complement(2585..3313)
                     /locus_tag="VIF08_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134765.1"
                     /translation="MAFEERGGWVIASDRVLRLLCWALILFALATAALQLTVSQNPFG
                     TPVPDGTDFVDRLLSYRSDDQKILGLALVSGLAGAGLYLMGSLIGIALRRLAPNGAAG
                     DVMAGLFVIGGIAGVVSQLMYVANANLATSGYCDCGYKTYEVIGQAYALEAGWALQNW
                     VNLGGLVIVGIAAAFAGWVVNLSRDWRILSYLIALGALAGAALRIFGAYQASDITLGI
                     TAGIAVPIWAFLLARGSSRLTARA"
     gene            3401..4837
                     /gene="lpdA"
                     /locus_tag="VIF08_01910"
     CDS             3401..4837
                     /gene="lpdA"
                     /locus_tag="VIF08_01910"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY8134766.1"
                     /translation="MAVGSSNSFDLIVLGGGTGGYSAAFRAGQLGLKVALVDQQPKLG
                     GTCLHWGCIPTKAMLEAAELYERIAHGAADFGVKATDVSFDYAVAAKRRQAVVDRLAK
                     GLDALAKKNKVEVIRGTGTLNGGKRVRVVTLDADGKPDGERPLDATDVILATGSRVKS
                     LPGLEPDGNQIITSDEATTGNTLPKSIIVVGGGAVGVEFASFYKDAGVDVALLEYLPR
                     LTPLEDAEISKELERSFRRRGINVITGARFDTAAVKKDEAGVSVMVGKEGEEPKEVRA
                     EQMLVATGRAPNSDNVGLESTRAKIEKGFVQVDARMRTAEPHLYAIGDLIGGLLLAHV
                     AAHEGIAAVHAIAGAEAEKVDYLTMPRATYSRPQIASIGRSQEEAEAAGMKVRVGRMP
                     FQAVGKALIRGEHEGFVKVVADATTDQLLGVHMIGAGVTDLIAEASAAMLLEATAWEV
                     GNAVHPHPTLSEALGEAALAVDGKSINF"
     gene            4862..6025
                     /locus_tag="VIF08_01915"
     CDS             4862..6025
                     /locus_tag="VIF08_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY8134767.1"
                     /translation="MATTKPATAKSDTPGEGPAETPADSATDSQALGANLGLTRDDLL
                     EMYRLMAVARAVDERMWILNRAGKIPFVISGQGHEGAQVALAYALKKGHDWMVPYYRS
                     VASLITFGMSSREIMLAQFARGVDPSSGGRQMPGHYGIVERNILSVSSPVATQLLHAT
                     GIAWAAKLRKTDQVTITYIGEGSSNQGDFHEGLNFAGVHRLPVIYVVENNGYAISVPM
                     SLQSAVADIAIRGAGYNMPGVIIDGVDVLACYKAGKEAIDRARRGDGPTLIEAKVTRL
                     TAHSSDDQQTKYRSAEELESEKGRDPLPRFRQQLVDAGFLDEATEEKMVAEARKVVED
                     ATDWAESQADPDPATAQQKVYADTPPARMDDPLWTGTRFIGDGGPWGALKGEG"
     gene            6040..7014
                     /locus_tag="VIF08_01920"
     CDS             6040..7014
                     /locus_tag="VIF08_01920"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY8134768.1"
                     /translation="MTFIESVRTTLADEMRRDDSIIVLGEDVGKKGGVLLATDGLFDE
                     FGGDRVLDTPLSESMIVGVSIGAAVAGLRPVPEIQFADFIFPAFNQILSEAARMRFRS
                     NGAFSCPMTLRAPYGGGVHGALYHSQSVEAFFAHIPGLKVVIPSTPYDARGLLRTSIR
                     DDDPVLFFEHKKMYRSVRGDVPDGDYTIPIGQAKVTREGTDVTLIAYGLMAQYALEAA
                     EILASDGVNAEVVDVRTLRPLDGETLLGSVRKTGKAVVIYEDNRFGGYGAEIAAILAE
                     EAFESLDGPIVRVTGPDVPGVPYNHVLEDWFMPNPERIAEAARRLAAY"
     gene            7037..7504
                     /locus_tag="VIF08_01925"
     CDS             7037..7504
                     /locus_tag="VIF08_01925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8134769.1"
                     /translation="MATVELIPMTDAAYAVWRDRSVREYAAEHVKSGNWTLEEAPANA
                     EAQFAQILPAGLATTGQFLWTVRDSAGDDVGILWVGTERRPGHAFIYDIEIADDRRGQ
                     GFGTAAMLALEEWARANGIATIGLHVFGHNTGAWQLYKRLGYVETNVQMEKRL"
BASE COUNT         1276 a         2639 c         2555 g         1057 t
ORIGIN      
        1 ctcgacgcga tccgcctcgc ggtctacgcc atctgcggct ggctggccgt gctcgccctg
       61 gggtgcgttg tcggcgggct ctacctctac cgactgggcg gaaggggccg actacccgcg
      121 gagtgaatca gccgcggacc agtccgcgcg acttgagccc cgatcagccc cggccgtctt
      181 cccgcggcgg ctgctggttt ccagtcgacc atggcccatt cgccggcggc gccgggattt
      241 ggcccggcca ggcggacatc ggggcctgcg gctccggcgc gttgagcggg cgcagcgtgc
      301 gcctgagcgg ctcgtcatcg tcttgcgacg ctgactcctc tgcaacgaaa tcgttctcgg
      361 ggtcctggct atcgaggccc atgcgcttgc gtcttttgcg aatgaactcc tcgggacccg
      421 cgaactgcag cagaacgaac atgaggagcc cggcaccgat acccaggaga tacaggccgc
      481 tgccgatggc caggcccacg cctgccgcaa cccacagtcc cgccgcggtc gtgagaccga
      541 cgacgtgcac cttggtgtgc aggatcgcac ccgcgcccag gaagccgatg ccggtgatga
      601 ccccgcccac gatgcgtgat gcgtagtcga gagagccgaa gccgtagatc gccgtgacgg
      661 tgaacatcgc cgcgcccagg ccaaccaggc cgtacgtgcg gatgccggcc gccttgccgg
      721 cgagctcgcg ctcaaggccg atcaggccgg cgaccaggcc ggccagggca aggcggatga
      781 gcgccgttac ctgcggatcg gcaagaaagg cgtcgaggtt ggtgggcatg tgcgcgtgca
      841 agtgtgcggt gtcggagcgt gtacatcaac agcgctgtgt tgcatcccgg caccggtcct
      901 acgatgggac catgccagtg gccgatgaga cgctacccgc caccgacgcg cgcctggccg
      961 atccggcgct ggatctgtca gggctggcgc gagtctgggc ggcgtacggt gcgcggcgcg
     1021 cgatgaccgc ggagcagatg gttggcgcgg accgcaaggc gcagcgcctg ggggtcgcgg
     1081 ggcgcgacct gatggaacag gcgggcacgg ccgtcgccgt cgcggcccgg gcgctggtca
     1141 actcgaccga ccggccgtcg tccggccacg tgctgatcct cgccggcccg ggcaacaacg
     1201 gcggcgacgc gttcgttgcc gcgcggcacc ttgccgaggc cgggatccgc tcgtacgtgg
     1261 tgctggtcag cgcggccacc aaaccgggca cgcccgacgc cgctgccaac tgggcgcgcc
     1321 tcgaaggcgt tggcgaggtg cagcgcatgc acgcgacgag cggccgcgag gtggcgatcc
     1381 tgctcaacgg gatcgagcgc gcggcgctgg tcatcgatgg gctgctcggg accggcgtcc
     1441 gcggcgcgct gcgcgagccg gtggcccagg cggtcgagct atgcctcgcc gctcggcggg
     1501 ccggcgtccc catcctgtcc atcgacacgc ccaccgccct cgacctgacc tcgggcgaac
     1561 cgtcggagcc gaatgtccag gccaacctca cggtgacctt ccaccgaccc aaacaagggc
     1621 tgcagacgcg caccggtcgg gccctcgccg gcggggtgtt ggtggcgccg atcggcatcc
     1681 ccgtggaggc cgaccatgac tgatcccgct tcgaatccag gacccgattc gccgcccggc
     1741 gctcgcgccc gtgacctgct ccttgcgctg gaagtcgccg ggttgatcgt ggtcggcgtg
     1801 ctcgtctttg cctgggtgac ggaccaggtg cccgtgctcg gcgaggccct gctcttcgag
     1861 ccgctggtca tcatcgccct cgtggcggtg acgatcgtga tcctcgcccg cgctctctgg
     1921 ccgcgccgct agccgtcccc tcgggcgacc gggtcgaagc gacgctcttc gccgcgcagc
     1981 aggcgctgga tgttgtcgtg gtgcgcgacc cacacgatcg ccgtgccgat cgccgcaaaa
     2041 aggaagtgtg gccacacgtt gtagcccagc agcaccagga tgccgatgat gatcgtgccg
     2101 cccagcgtgc cgatcagcga gcccagcgag acgtagcgcg tcagcaggat tggcccaaag
     2161 aagaagggca cggccagcgg gatggccagg ggcgccatcc agaccgtcgc gccaatgccc
     2221 gtcgccacac cgcggccgcc atggaagcgc agccagacgg atgcccacgc gccaaccact
     2281 gccgccacgc cggtcagcgc cacgcccaag tcggacgcgc caagcagcgc cgcggccgcg
     2341 accggcacga agcccttcaa gacgtcgagc gtcgccacca gcccggcccg ccacgcgccc
     2401 atggcccgca acgcgttcgt gcccccggtg cggcccgaac cgactgtgcg cgggtcgcgc
     2461 gcgccggtga gtcgggcgac gatgacgccc ataggcaatg agccaccgag atagccgatc
     2521 gctatgaaca gcccgtcaag ggcgatctgc ggcagcatct ggccggcccg cggtcagtcg
     2581 ttgatcaggc ccgcgcggtc agtcggctgg acccgcgcgc cagcaggaag gcccagatcg
     2641 gcaccgcgat gccagccgtg atgccgagcg tgatgtcgga cgcctggtag gcaccaaaga
     2701 tgcgcagcgc ggcaccggcc agggctccca aagcaatgag gtacgacagg atgcgccagt
     2761 cgcggctcag attgaccacc cagccggcaa acgcggccgc gatccccacg atcacgagcc
     2821 cgccgagatt gacccagttc tggagcgccc acccggcctc cagcgcgtaa gcctggccga
     2881 tgacctcgta cgtcttgtag ccgcagtcgc aatagcccga tgtcgcgagg ttggcgttgg
     2941 cgacatacat cagctgcgat acgacaccgg cgatgccccc gatgacgaag agcccggcca
     3001 tcacgtcacc ggcggcgccg ttcggcgcga gacggcggag cgcgatgccg atcagcgagc
     3061 ccatcaggta caggccggcg ccagccaagc ccgacacgag cgccaggccg aggatcttct
     3121 ggtcatcgct gcggtagctc agcagccggt cgacaaagtc ggtgccgtcc gggaccggag
     3181 tgccgaacgg attctgcgac acggtcagct gcagcgccgc cgtcgccagc gcaaagagaa
     3241 tgagcgccca gcacagcagg cgcagcacac gatctgacgc gatcacccag ccacctctct
     3301 cctcaaacgc catcccgcaa cggctattcc gcgggccagt gtacgtggcg ctgctaccat
     3361 caaatgacat gattcccacc gtccttgagg ccggctgaac gtggctgtcg gctcctcgaa
     3421 ttcgttcgat ctgatcgtcc tcggcggtgg caccggcggc tactcggccg ccttccgcgc
     3481 cggccagctg gggctcaaag tggcgctcgt ggaccagcag cccaagctcg gcggcacgtg
     3541 cctccactgg ggctgcatcc cgaccaaggc gatgctggaa gcggcggagc tatacgagcg
     3601 catcgcccac ggcgcggccg actttggcgt gaaggccaca gacgtgagct tcgactacgc
     3661 ggtcgcggcc aagcgccgcc aggcggtggt cgatcggctg gccaagggcc tggacgcgct
     3721 cgccaagaag aacaaggtcg aggtcatccg cggcactggc acgctcaacg gcggcaagcg
     3781 ggtgcgcgtc gtgaccctcg acgcggacgg caagccggac ggtgagcggc cgctcgacgc
     3841 gacggacgtg atcctcgcca cgggctcgag ggtcaagtcg ctccccggcc tcgagccgga
     3901 cggcaaccag atcatcacca gcgacgaggc cacgaccggc aacacgctgc ccaagtcgat
     3961 catcgtcgtc ggcggcggcg cggttggcgt cgagttcgcg tcgttctaca aggacgcggg
     4021 cgtggacgtg gcgctgctcg aatatctgcc gaggctcacg cccctcgagg acgcggagat
     4081 cagcaaggag ttggagcgca gcttccggcg gcgcggcatc aacgtcatca cgggcgcgcg
     4141 ctttgacacc gccgcggtga agaaggacga ggccggcgtg tccgtgatgg tcggcaagga
     4201 aggggaggag cccaaggagg tgcgcgccga gcagatgctc gtcgccactg gccgcgcccc
     4261 caacagcgac aacgtcgggc tcgaatcaac acgcgccaag atcgagaagg gcttcgtcca
     4321 ggtggacgcg cgaatgcgca ccgctgagcc gcacctctat gcgatcggcg acctgatcgg
     4381 ggggttgctg ctggcgcacg tcgccgcgca cgagggcatc gcggcggtcc acgccatcgc
     4441 gggcgcagag gcggagaagg tcgactacct cacgatgccg cgcgcgacct actcccggcc
     4501 gcagatcgca tcaataggca ggagccagga agaggctgag gccgccggca tgaaggtcag
     4561 ggtcggcagg atgcccttcc aggccgtcgg caaggcgctc atccggggcg agcacgaggg
     4621 cttcgtcaag gtcgtcgccg acgccaccac ggaccagctg ctgggcgtgc atatgatcgg
     4681 cgccggggtg accgatctaa tcgcggaggc atcagcagcg atgctgctgg aagcgaccgc
     4741 atgggaggtc ggcaacgccg tccatccgca ccccaccctg tcggaggcac tgggcgaagc
     4801 ggccctggcg gtcgacggca agtcgatcaa cttctagacc cgcccgataa actccgcgcc
     4861 gatggcaacg accaaaccag ccaccgcgaa gtccgacacc ccaggcgaag gcccggccga
     4921 gaccccggcc gacagcgcga ccgattccca ggcgctcggc gcaaaccttg gcctgacgcg
     4981 cgacgacctg ctcgagatgt accgcctgat ggcggtcgcg cgtgcggttg acgagcgcat
     5041 gtggatcctc aaccgcgccg gcaagatccc gttcgtcatc agcggccagg gccacgaggg
     5101 cgcgcaggtt gcgctcgcct acgcgctcaa gaagggccac gactggatgg ttccgtacta
     5161 ccgctccgtc gcgagtctca tcacgtttgg catgagctcg cgcgagatca tgctcgccca
     5221 gttcgcgcgc ggcgtggatc cgtcgagcgg cggtcgccag atgcccggcc actacggcat
     5281 cgtcgagcgc aacattctgt cggtctcgtc gcccgtggcc acccagctgc tccacgcgac
     5341 gggcatcgcc tgggcggcca agctgcgcaa gaccgaccag gtgacgatca cctatatcgg
     5401 cgagggctcg tccaaccagg gcgacttcca cgagggcctc aacttcgcgg gcgtgcaccg
     5461 cctgccagtc atctacgtgg tcgagaacaa cggctacgcg atcagcgtgc ccatgtcgct
     5521 ccagagcgcc gtggctgaca tcgccattcg cggcgccggc tacaacatgc ccggcgtgat
     5581 catcgacggc gtcgacgtgc tcgcttgcta caaggctggc aaggaggcga tcgaccgcgc
     5641 tcgtcgcggc gatggcccga cgctgatcga ggccaaggtc acccgactca ccgcccactc
     5701 ctcggacgac cagcagacga agtaccgctc cgcggaggag ctcgagtcgg agaagggccg
     5761 cgacccgctg cccaggttcc gccagcagct ggtcgacgcg ggcttcctcg acgaggcgac
     5821 agaggagaag atggtcgcgg aggcgcggaa ggtcgtagag gatgcgaccg actgggccga
     5881 gtcgcaggcc gaccccgatc cagccacggc ccagcagaag gtctacgcag atacgccccc
     5941 cgcgcgcatg gacgatccac tctggaccgg cacccggttc atcggcgacg gcgggccgtg
     6001 gggcgcgctg aagggcgagg gctagtcgcg atggccgtca tgacgttcat tgagagcgtc
     6061 cgcaccacgc tcgccgacga gatgcggcgc gacgactcga tcatcgtgct gggtgaggac
     6121 gttggtaaga agggcggcgt cctcctcgcg accgacgggc tatttgacga gttcggcggc
     6181 gaccgcgtgc tcgacacccc gctttcggaa tccatgattg ttggcgtctc aattggcgcc
     6241 gccgtcgccg gcctgcggcc ggtcccggag atccagttcg ccgacttcat cttccccgcg
     6301 ttcaaccaga tcctgtcgga ggcggcgcgg atgcgcttcc gctccaacgg ggcgttctca
     6361 tgtccaatga cgctgcgcgc accgtatggc ggcggcgtgc acggcgcgct gtatcactcg
     6421 cagagtgtcg aggcgttctt cgcccacatc cccggcttga aggtcgtcat cccgtcgacg
     6481 ccgtacgacg cgcgcgggct gctgcgcaca tccatccgcg acgacgaccc ggtgctgttc
     6541 ttcgagcaca agaagatgta ccgctccgtg cgcggagacg tgccggacgg cgactacacg
     6601 atccccatcg gccaggccaa ggtgacgcgc gaggggacag acgtgaccct catcgcgtac
     6661 ggcctgatgg cgcagtacgc cctggaggcc gcggagatcc tggcgtcgga cggcgttaac
     6721 gcggaggtcg tcgatgtgcg cacgctccgt cccctggacg gggagaccct gctggggagc
     6781 gtccgcaaga ccggcaaggc ggtcgtgatc tacgaggaca accgcttcgg cggctacggc
     6841 gcggaaatcg ccgcgatcct ggccgaggag gccttcgagt cgctcgacgg acccatcgtt
     6901 cgcgtcacag gccccgacgt cccgggcgtg ccctacaacc acgtcctcga ggactggttc
     6961 atgcccaacc cagagcgcat cgccgaagcc gcccgccgcc tcgccgccta ctgaccggcg
     7021 cccgtaccac cggcgcatgg ccacagtcga gctgatcccc atgaccgacg ccgcctacgc
     7081 cgtgtggcgc gaccgcagcg tccgcgagta cgccgccgag cacgtgaagt ccggcaattg
     7141 gacccttgaa gaagcacccg ccaacgcgga ggcgcagttc gcgcagatcc tgcccgccgg
     7201 cctagcgacg accggccaat tcctgtggac ggtccgcgac tccgccggcg atgacgtggg
     7261 catcctgtgg gtcggcaccg aacggcgacc cggccatgcg ttcatctacg acatcgagat
     7321 cgccgacgac cgacgcggcc agggcttcgg cacggcggca atgctcgccc tcgaagagtg
     7381 ggcgcgcgcg aacggcatcg ccaccatcgg cctgcacgtc ttcggccaca acaccggcgc
     7441 gtggcagctc tacaagcgcc tcggctacgt cgagacgaat gtgcagatgg aaaagcgcct
     7501 ctagccaccc gcctgcgtcg tcgcccg
//
LOCUS       DATBRX010000054         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3479718, whole genome shotgun sequence.
ACCESSION   DATBRX010000054 DATBRX010000000
VERSION     DATBRX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3479718"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VIF08_01930"
     CDS             complement(<1..1820)
                     /locus_tag="VIF08_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134770.1"
                     /translation="MDRHSRVNRIVAAAFALALLALQPASLALAESPLPSPSPQTNTD
                     IGMEAHALLGGHVRPGAWTAVAVHLTNNGPAIDGELRIGSGTGTTSQYGLEVHLATPA
                     NQEVTLYAQTQIFGSKINVDLAVGDQVIARQTVTIRSHDSYSPIVAVVAEQPQGLLPQ
                     INAALVNPNFATSTVITLGAADLPPRVEAWAAIDRLVWQDVDAADLTPQQAEALRLWL
                     GAGGQLIIVGGTTGTGTVRGFDGPLDPVTGLPQDPILPFDPTHTVDVAAADLVPLVGA
                     LPSDATTIPAVAGLLRGGSILARSGDDVIAAQQSYGRGMVSLIGFNPAERWLTDGATA
                     SALWHRLLPAVTSQALNPLQLTDDSQIVYAMQNVPQVALPPIEQLFILLVAYIALIGP
                     INYLVLRRLDKREWAWITIPVLVVVFALGSYGLGASLKGSDVIVNEVGVVRASQGSGR
                     GIGQVYVGIYSPSRRSFQVSIPGGALLSNPISQVQNTGSEQPLDVLFGSAASHLRNFE
                     VGFGVVRGFRAEAPADAPQIESDLHLGSGRLQGTITNRSDSTLENIAVVFGGGVAIVE
                     SLEAGKSSTIDINITTSNQYGYALSERIFGSSFPRDAATAR"
     gene            1963..>2743
                     /locus_tag="VIF08_01935"
     CDS             1963..>2743
                     /locus_tag="VIF08_01935"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY8134771.1"
                     /translation="MPQLRPFRGLRYTPAAVPDLAAVLCPPYDVISTDERRGLIERDP
                     YNAVRLELPDSYDDATALLASWQDAGVLARDERPLVYIYEQRYVVGDSERVARSFFCR
                     LRLEPYGPGSGVRPHEHTLAAPKKDRFHLLEATRVNLSPVLLLYDDARGGAASASLLD
                     ELTGAPPTASAAGPGGVGQRLWAIDPEAVPAANELLELAGRAPLTIADGHHRYETALR
                     YRATSGAPPDADHVLALLYDAHSGGLALRPWHRVVADSLAAR"
BASE COUNT          423 a          911 c          980 g          429 t
ORIGIN      
        1 cgcgccgtgg ccgcgtcgcg cgggaagctc gagccgaaga tgcgctccga cagcgcgtag
       61 ccgtactggt tgctcgtggt gatgttgatg tcgatcgtgc tgcttttgcc cgcctcgagg
      121 ctctcgacga tggcgactcc gccaccgaac acgaccgcga tgttctcgag cgtgctgtcc
      181 gaccgattcg tgatggtgcc ctgcagccga cccgagccca ggtgcaggtc gctctcgatc
      241 tgcggcgcat ctgccggcgc ctccgcgcgg aagccgcgca ccacgccaaa gccgacctcg
      301 aagttgcgca ggtgggaggc cgcgcttccg aacagcacgt cgagcggctg ctcggacccg
      361 gtgttctgca cctggctgat gggattcgac aacagcgcgc cgcccgggat gctgacctgg
      421 aatgagcgcc ggctgggtga gtagatgccg acgtagacct ggccaatgcc ccggccgctg
      481 ccctgcgatg cgcgtacgac accaacctcg ttgacgatca cgtcgctgcc cttgagcgat
      541 gcgccgaggc catatgagcc gagggcgaac acgacgacca gcacggggat ggtgatccac
      601 gcccactcgc gcttgtcgag gcggcgcagc acgaggtagt tgatggggcc gatcagcgcg
      661 atgtaggcca cgagcaggat gaacagctgc tcgatgggcg gcagcgcgac ctgcgggacg
      721 ttctgcatgg cgtagacgat ctgcgagtcg tcggtcagct ggagcgggtt gagcgcctgg
      781 ctggtcacgg ccggcaacag ccgatgccac agcgccgatg cggtcgcgcc gtcggtcagc
      841 cagcgctcgg ccgggttgaa gccgatgagc gacaccatgc cgcggccgta tgactgttgc
      901 gcagcaatga cgtcgtcgcc gctgcgggcg aggatcgagc caccgcgcag gagaccggcg
      961 acggccggga tcgtcgtggc atcggacggc agtgccccga cgagcggcac gagatcggcc
     1021 gcggcgacat cgaccgtgtg cgtcggatca aagggcagga tcgggtcttg gggcaggcca
     1081 gtcaccgggt cgagcggtcc gtcaaagccg cggacggtgc cggtgccggt tgtacccccg
     1141 acgatgatga gttgaccgcc tgcgccgagc cagagacgca gcgcctcagc ctgttgcggc
     1201 gtgagatccg ctgcgtccac gtcctgccag acgaggcgat cgatcgcggc ccatgcctcg
     1261 acgcgcggcg ggagatcggc cgcgcccagg gtgatgacgg tggaggtggc gaagttgggg
     1321 ttgaccaacg cggcgttgat ctgggggagc aggccctgcg gctgctcggc cacgacagcc
     1381 acgatcgggc tgtaggagtc atgcgaacgg atcgtcacgg tctggcgcgc gatcacctga
     1441 tcgccgaccg cgagatccac gttgatcttg ctgccgaaga tctgggtctg agcgtacagc
     1501 gtcacttcct ggttcgcggg ggtggcgaga tggacctcca gcccgtactg cgaggtggtc
     1561 cccgtgccgg aaccgatgcg cagctcgccg tcgatcgccg ggccgttgtt cgtcaggtgg
     1621 accgcaaccg cggtccaggc accgggtcgg acgtggccgc ccagcagcgc gtgcgcctcc
     1681 atcccgatgt ctgtgttcgt ctggggcgag ggcgagggga gaggtgactc ggccagcgcg
     1741 agagaggcgg gttggagcgc gagcagggca agcgcaaacg ccgcggccac gatgcggttc
     1801 accctggagt gacggtccat cagccctcag attaggtggt tgtccgtcca tgacggtgcg
     1861 cgtgccacga aagttgcacg gccggcggct ccagcggccc tcatctggga catggcttcg
     1921 acttgcaggg cgcgactgcc cgcgcggcta ccatcccttg acgtgcccca gctacggcca
     1981 tttcgcggtt tgcgctacac cccggccgcc gtgccggacc tcgcggccgt cctgtgccca
     2041 ccgtacgacg tcatctcaac cgacgagcgg cgtggcctga tcgagcgcga cccgtataac
     2101 gcggtgcggc tcgagctgcc cgattcgtac gacgacgcga cggctctgct cgcgagctgg
     2161 caggacgcgg gcgtgctcgc gcgcgacgag cggccgcttg tctacatata tgagcagcgc
     2221 tacgtcgtcg gggacagcga gcgggtcgcg cgttcgttct tctgtcggct gcggctcgag
     2281 ccgtacgggc cgggcagcgg ggtgcggccg cacgagcaca ccctggccgc gcccaagaaa
     2341 gatcgcttcc acctcctcga ggcgacgcgc gtcaacctgt caccggtcct gctgctgtac
     2401 gacgacgcgc gcggcggcgc ggcgagtgcc agtctcctgg acgagctcac aggggcgccg
     2461 cccacggcga gcgcggccgg cccgggcggc gtcggccaaa ggttgtgggc gatcgatccc
     2521 gaagcggtac ccgcagccaa cgagttactg gagctggccg gccgcgcccc gctgaccatc
     2581 gccgatggcc accatcggta cgaaaccgcg ttgcgctatc gagctacctc cggcgctccg
     2641 ccggacgcgg accacgtgct ggccctgctg tacgacgctc attccggcgg cctcgctctg
     2701 cgaccgtggc atcgggtcgt cgcggactcg cttgcagcac gat
//
LOCUS       DATBRX010000055         6006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3479805, whole genome shotgun sequence.
ACCESSION   DATBRX010000055 DATBRX010000000
VERSION     DATBRX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3479805"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3034
                     /locus_tag="VIF08_01940"
     CDS             <1..3034
                     /locus_tag="VIF08_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8134772.1"
                     /translation="PMAFIGRERELAVVGTALQRSSQRELTRVAISGPLGIGTTRLLD
                     ELEARIAKAADLAKVTIVRARSLEPEAGIAYAGLRRALGKEFDAAVAARPALDAPDQR
                     GARVRESLLRLFESKAANGQVCLIVEDLEHADPGTLELIRMVLRLRRRMALTLIVTFH
                     GDEIGRGHGAAGLRDELLGNPGVEQIALEPMSADELLALVEAMTGDRPTLSFMAAVTE
                     GSRGNPLVASQLVEASQRQAGIRLSDPHEEIIHARLAQLERPTLRALRLLAAARVPMA
                     TDQLARVRLPDGHLTRTAVAELGESGLVAGDGADGGLLSLHQLVAEAVDHSTLPGELH
                     ELHAALAQANGASPAIAAWHWSRAFKPVKARDAYYAAAALAEEIEPGPTTMADYNAAL
                     ELDIDGEADADTIARAAAAAAAAGAFRHAATLLEQAIERLADGRAERLLSPRASAEAR
                     DRAALLSEELGRYRRESGDIVGGRAAIEQALELAATAPASTRARVLASLAQDLMLADY
                     EGHYEDSAKRAEEARRVARSAGADGLADLAHATDTLAVDLGFGRGAINKALALLDEAI
                     DAARKAGRLDELMRCYANKTTLLDLDTRREEALAAVKEGIAEAERGGLALTYGSFLRG
                     NAADILFQLGRWAESEAECRAALEFPPAGVAWFSPILYLGLVLVESRADEEAARLVGQ
                     TLLELEAVPAGQWSALVLRSAVSLALWRGEAADAVAAAESGWAAVAETDDAHQMAFAA
                     ATVLEACAAAAERGRAKRDYSAVADAGALAERVLAAATEAMAKPGLPRTVGARKEAEL
                     YLATARAHSERVRGRARPEPWAKLAEAWAAQSVPYNVARARYWQTAALLTDRSRRSEA
                     RKSITEAWKIAGKLPAAPLRKALAELGVRGNIRLPEDKLVAIPIQPEVGTKDLVAVGP
                     GPVSGVVPADFAARIGTGHRHPASGRFGLSPRESSVLVILTEGRTNREIAERLFISER
                     TVAVHVRRILQKMGVKGRVEAAGVAIRLGLVPDAKVRR"
     gene            3085..3546
                     /locus_tag="VIF08_01945"
     CDS             3085..3546
                     /locus_tag="VIF08_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8134773.1"
                     /translation="MHPQSKLIRFALPVSVALILSACGGAGPGWTYAPLGPSAPPTSA
                     ASPTPAATPSVTLAVKTTGASPLTFNPATLDAPANTVVQVDYLNDSPVPHNINFFAGA
                     DSTAESLGKTAVKTGPGATESVIFTTPTAPGDYYFWCDVHGAAMSGTLHVQ"
     gene            3601..5130
                     /locus_tag="VIF08_01950"
     CDS             3601..5130
                     /locus_tag="VIF08_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8134774.1"
                     /translation="MSLNPSPAIAALLSFLFPGVGQIYAGDLRKGVLWALPMLAFILA
                     IIWLLIGPKIALVGLITNPQTRVAILVLNVAFFLYHVAAMVDAYSVAKAERYRGFSRT
                     SATAPVVLAALVAVTMLLHGVPEVIGMDVNNALNIIGQGHQVVPSFTPRPTLAPSTTP
                     LVTPTPDVTATPDASGSPVPTPTGPAGPSPTPRACPQTDFAGWGPASDGRLNILLSGS
                     DSRSDEGASTNSVRTDSMMLLSIEIATCKAALFSFPRNMNCDVRYPEWFHIPLENGQD
                     YPDCLNYLWRSAAANPNNYPGSVGIGPECQAQFDCERGWRALTGAIQNFANQQIDGVV
                     AVNLKGFVALVEALPGHGVWIDVPYALHDDVYHDSQQRPIVVDFQPGCQFLDGEQALA
                     FARSRHQDSDYQRARRQQIFLQQVRKQLDPLALLGSMDSLLLAAQENLFMSFQQSDFQ
                     YLAQIASHVDADRLYRYDFAPAQLNDFHNMDDLAAKIYNIFSEPEPKPDTNNEDPCPP
                     R"
     gene            complement(5141..5788)
                     /locus_tag="VIF08_01955"
     CDS             complement(5141..5788)
                     /locus_tag="VIF08_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY8134775.1"
                     /translation="MFDLDGTLVDTVEARIQAWLEVFKEVRIKAERDHVARLIGSDGK
                     RLAHEVADVAGQPINDDKAEAIDKRCGEIYDRINTDPRPTAGAHELLAALSAGNLPWA
                     VATSSRAEQVGTSIDALRLTRKPRIIDGSHVEHAKPAPDLLLLAAEQLDLPASACWYV
                     GDSTWDMQAATAARMTAVAVPYGAVSGETLTRAGANVVTTLLQLKSDLERRRLIP"
     gene            complement(5824..>6006)
                     /locus_tag="VIF08_01960"
     CDS             complement(5824..>6006)
                     /locus_tag="VIF08_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134776.1"
                     /translation="ERGTYTFNVTVGAASGSPLPTPPPNDGEPVTDSKDPLVAVGLAA
                     IAIVLILAFIFFRRRR"
BASE COUNT          925 a         2109 c         2060 g          912 t
ORIGIN      
        1 gcccatggcg ttcatcgggc gtgaacgcga gctggccgtg gtggggacgg ctctgcagcg
       61 tagttcgcag cgcgagctga cccgagtcgc gataagcggt ccactcggga tcggcactac
      121 gcgcctgctc gatgagctcg aggcacgcat cgccaaggcc gcggacctcg cgaaggtgac
      181 gatcgtccgg gcgcgttctc tcgagcccga ggctggcatc gcctacgccg gcctgcgccg
      241 cgcgctgggc aaggagttcg acgcggcggt ggccgcgcgg ccagccctgg atgcgcccga
      301 ccagcggggc gcgcgtgtcc gcgagagcct gctgcgcctc ttcgagagca aggcggcgaa
      361 cggccaggtg tgcctgatcg tcgaggatct cgagcacgct gacccgggca cgctggagct
      421 gatccgcatg gtcctgcggc tgcgccggcg gatggcgctg acgttgatcg tgacgttcca
      481 tggcgacgag atcgggcgcg gtcacggtgc tgccgggctg cgtgacgagc tgctgggtaa
      541 ccctggcgtg gagcagatcg ccctggagcc gatgtcagcc gacgagctgc tcgcgttggt
      601 cgaggcgatg accggtgacc ggcccacgct ctctttcatg gccgcggtga cggagggctc
      661 gcgcggcaac ccgctggtgg cgtcgcagct ggtcgaggcg agtcagcgcc aggcggggat
      721 ccggctgtcc gacccgcacg aggagatcat tcacgcccga ctggcccagc tggagcggcc
      781 gactctgcgg gcgctccgcc tgctggccgc cgcgcgggta cccatggcca ccgatcagct
      841 ggcccgggtc cgactgccgg acgggcacct gacccgcacc gcggttgccg agctgggcga
      901 gagcggcctg gtggcgggtg acggcgccga cggcggactg ttgtcgcttc accagctggt
      961 cgcggaggcg gtcgatcact cgaccctgcc cggcgagctg catgagctgc acgccgcgct
     1021 ggcgcaggcg aacggcgcct cgccggcgat agcggcctgg cactggtcgc gcgccttcaa
     1081 gccggtcaag gcgcgcgacg cctattacgc ggcggccgcc ctggcggagg agatcgagcc
     1141 gggtccgacc accatggccg actacaacgc cgccctggag ctggacatcg acggtgaggc
     1201 ggacgccgac acgatcgccc gcgccgcggc cgccgcggca gcggcgggcg ccttccgtca
     1261 tgcggcaacg ctgctcgagc aggccatcga gcgcctcgcc gacggccgcg ccgagcgcct
     1321 gctgagcccg cgtgcgtcgg ccgaggcgcg cgatcgcgcc gcactgctga gcgaagagct
     1381 gggtcgctac cgccgcgagt cgggcgacat cgttggcggc cgcgcggcga tcgagcaggc
     1441 gctggagctt gccgccaccg cgcctgcgtc gacacgggcg cgcgtcctgg cgtcgctcgc
     1501 ccaggacctg atgctggccg actacgaggg ccactacgag gacagtgcca agcgcgccga
     1561 ggaagcgcgc cgcgtggcca ggtcggcggg cgccgacggg ctggccgatc tagctcacgc
     1621 caccgacacg ctcgcggtcg acctgggctt tggccgcggc gcgatcaaca aggcgctggc
     1681 gttgctcgac gaggcaatcg atgccgcgcg caaggccggg cggctcgacg agctgatgcg
     1741 ctgctatgcc aacaagacga cgctgctcga tctcgacacg cggcgcgagg aggcgctcgc
     1801 ggcggtcaag gagggcatcg ccgaggccga gcgcggtggt ctggcgctga cctacggctc
     1861 gttcctgcgc ggcaatgcgg cggacatcct gttccagctg ggccgctggg cagagagcga
     1921 ggcggagtgc cgcgcggcgc tcgagttccc gccggccggc gtcgcatggt tcagccccat
     1981 cctgtacctg ggcctggtcc tggtcgagtc gcgcgccgat gaggaagcgg cgcggctcgt
     2041 gggccagacg ctgctcgagc tggaggccgt gcccgccggc cagtggtcgg cgctggtgct
     2101 gcgcagcgcg gtcagtctcg cgctgtggcg cggcgaggcc gccgacgccg tcgcagcagc
     2161 ggagagcggc tgggcggccg tcgccgagac cgacgacgcg caccagatgg cgttcgccgc
     2221 agctaccgtc ctcgaggcct gcgccgcggc cgcagagcgc ggtcgcgcca agcgcgacta
     2281 ctcggccgtc gctgacgcgg gcgcactcgc cgagcgtgtc ctagccgccg cgacggaggc
     2341 gatggccaag ccgggattgc ccaggaccgt cggcgcacgc aaggaggccg agctgtatct
     2401 cgcgaccgcg cgcgcccaca gcgagcgagt ccgcggccgc gcgcgcccgg agccgtgggc
     2461 gaagctggcc gaagcctggg ccgcgcaatc ggtgccgtac aacgtcgccc gcgcgcgcta
     2521 ctggcagacc gccgcgcttc tgactgaccg gtcgcggcgc agtgaagcgc gcaagtcgat
     2581 aacggaggcg tggaagattg ccggcaagct gccggccgcg ccattgcgca aggcgctcgc
     2641 cgagctcggc gtgcgcggca acatccggct gcctgaagac aagctggtcg cgatcccgat
     2701 tcagcccgaa gtcggcacaa aggacctggt ggctgtgggc cccggcccgg tgagcggtgt
     2761 ggtccccgcc gacttcgcgg cccggattgg cacaggccat aggcatccag cgtccggtcg
     2821 gttcggcctc agcccgcgcg agtcatcggt gctggtgatc ctcaccgaag gccgcaccaa
     2881 ccgcgagatc gcggagcggc tcttcatcag cgagcgcacg gtcgcggtgc acgtccggcg
     2941 catcctccag aagatgggcg tcaagggccg cgtggaggcc gccggcgtcg ccatccgact
     3001 ggggctcgtg cccgacgcga aggttcggcg ctgaccgcgc tccggccccg gcgccattcg
     3061 tcgcaatcgg ccagaatggc ggtgatgcac ccccagtcaa agctgatccg tttcgcgctg
     3121 ccggtcagcg tggcgctcat cctgtccgcg tgtggcggcg ccggtcccgg ctggacctat
     3181 gcaccgctcg gcccgtctgc gccgccaacg tccgcggcga gccctactcc ggccgcgacg
     3241 ccatcagtca cgctcgccgt caagaccacc ggcgcgagcc cattgacctt caatcccgcg
     3301 acgctggacg cgccggcaaa caccgtcgtc caggtcgatt acctcaacga ctcacccgtg
     3361 ccccacaaca tcaacttctt tgccggcgcc gactcgactg cagagtcgct gggaaagacc
     3421 gcggtcaaga ccgggccggg ggcaaccgaa tcggtcatct tcacgactcc gaccgcgccc
     3481 ggtgactact acttctggtg cgatgtccac ggcgccgcga tgtcgggaac gctgcacgtc
     3541 cagtaatcgg ctggccgcga aacggaacat gaccctgctc acattcgtca ggacgaagga
     3601 atgtcactga atccatcacc ggcaatcgcc gcgctgctct cgttcctgtt cccgggcgtc
     3661 ggccagattt acgccggcga tctgcgcaag ggcgtgctgt gggcgctgcc gatgctggcc
     3721 ttcatcctgg ccatcatctg gctgctgatc ggccccaaga tcgcgctcgt cgggctcatc
     3781 accaacccgc agacacgcgt cgcgatcctg gtactgaacg tcgctttttt cctgtaccac
     3841 gtcgcagcaa tggtcgacgc atattccgtc gccaaggccg agcgctatcg cggcttcagc
     3901 cggacgtcgg ccacggcgcc ggtcgtcctc gccgcccttg ttgctgtgac gatgctgctg
     3961 cacggcgtgc cggaggtcat cggcatggat gtgaacaacg cgttgaacat catcggccag
     4021 ggtcaccagg tggtccccag cttcacgccg cggcccaccc tcgcgccgag caccacgccg
     4081 ctagtaacgc cgacgccgga tgtcaccgcc acacccgacg ccagcggcag ccccgtgccc
     4141 acgccaacag gtccggccgg cccatcgccg acgccgcggg catgtccgca gaccgacttc
     4201 gccggttggg gtcctgcttc tgacggccgc ctcaatatcc tgctgtcggg ctcagactcc
     4261 cgatccgacg agggcgccag cacgaatagc gtccggaccg actcgatgat gctgctgtcg
     4321 atcgaaatcg cgacgtgcaa ggcggcgctg ttcagcttcc cgcgcaacat gaactgcgat
     4381 gtgcgctacc ccgagtggtt ccacatccca ctcgaaaacg ggcaggacta ccccgactgc
     4441 ctcaactacc tgtggcgctc tgcggccgct aacccgaaca actacccagg ttcggtaggc
     4501 atcggcccgg aatgccaggc gcagttcgac tgcgaaagag gctggcgggc actaaccgga
     4561 gccatccaga acttcgccaa ccagcagatc gacggcgtgg tcgccgtgaa cctgaagggc
     4621 tttgtcgcct tggtcgaggc actgcccggc catggtgtct ggattgacgt gccctacgct
     4681 cttcacgacg acgtctacca cgattcccag caacggccca tcgttgtcga cttccaaccg
     4741 ggctgccagt tcctcgatgg cgagcaggcg ctcgccttcg ctcggtcgcg ccaccaggac
     4801 tcggactatc agcgcgcgcg acgccagcag atcttcctgc agcaggtgcg caagcagctc
     4861 gatccgttgg ctctcctggg cagcatggac agtctcctgc tggctgccca agagaacttg
     4921 ttcatgtcat tccagcagtc ggatttccaa tacctcgccc agatcgcgtc gcacgtcgat
     4981 gcggacaggc tctaccgata cgacttcgcg ccggcgcagc tgaacgattt ccacaacatg
     5041 gatgacctgg cggcgaagat ctacaacatc ttctcggagc cagagccgaa gccggatacc
     5101 aacaacgagg atccctgccc gccgcgctag ggaacctggg ctatgggatc aggcggcgcc
     5161 gctccaggtc gctcttcagc tggagcaggg tcgtaacgac gttcgcgccg gcgcgcgtca
     5221 gcgtctcgcc actcaccgct ccatagggca ccgcgacagc ggtcatgcga gcggcggtcg
     5281 cggcctgcat gtcccacgtc gagtcgccca cgtaccagca cgcactggcg ggcaggtcga
     5341 gctgctccgc cgcgagcagc aacaggtcgg gcgcaggctt ggcgtgctcg acgtggctgc
     5401 catcgatgat gcgcggcttc ctggtgaggc gtagcgcatc gatcgacgtg ccgacttgct
     5461 cggcccggct cgaagtggcg accgcccacg gcagattgcc cgcgctgagc gcggcgagca
     5521 gctcgtgtgc gcccgcggtc ggccgcggat ccgtgttgat gcggtcgtag atctcgccgc
     5581 agcgcttgtc gatggcctct gccttgtcgt cattgatggg ctgaccggcg acgtccgcga
     5641 cctcgtgcgc caggcgcttg ccgtcagagc cgatcagccg cgcgacgtgg tcgcgctcgg
     5701 ccttgatgcg gacctctttg aagacctcca gccaggcctg gatgcgcgcc tcgaccgtgt
     5761 cgacgagcgt cccatcgagg tcgaacacca gcccgcgcgg atcggtcagc tcctcgagag
     5821 tgctcagcgc cgcctccgga agaagatgaa agccaggatc agaacgatcg cgatcgccgc
     5881 gagccccacg gcgacgagcg gatccttcga atccgtgacg ggctcgccgt cgttaggcgg
     5941 cggcgtcgga agtggagagc cgctcgccgc cccgactgtg acgttgaacg tgtaggtgcc
     6001 gcgctc
//
LOCUS       DATBRX010000056        10627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1469513, whole genome shotgun sequence.
ACCESSION   DATBRX010000056 DATBRX010000000
VERSION     DATBRX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10627
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469513"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VIF08_01965"
     CDS             <1..921
                     /locus_tag="VIF08_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY8134777.1"
                     /translation="RFAEPVVQPGWPANKVDLHSHSDRSDGVLPPTELYQHMTEVGLE
                     ICALADHDTLAGYRLLRDGIATGAITLGPRLIPAVEINSVADRALIELGIELEEGELH
                     ILGYGVDTDDGVFEAKLEGQRNARRTRLELMIERLRELGLPIDEQIAPALASEEAVGR
                     PHVARAMVAAGHVASVQQAFDEWIDRNGRAYVPRQGMRSRDAIDAIMAAGGIPVLAHY
                     PAAPEQPTLIGLLMEWGVRGLEVYYRRYLPATVSAMIDLAARLGVLATGGSDYHGDTM
                     SYAEATQTTFVPLAAGERLLEAIDTGVRAT"
     gene            918..2402
                     /gene="miaB"
                     /locus_tag="VIF08_01970"
     CDS             918..2402
                     /gene="miaB"
                     /locus_tag="VIF08_01970"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY8134778.1"
                     /translation="MTTRALPVLDVVRPARPARTDSAAVTPSLAEFMTDDLALPRFHV
                     WTLGCQMNHSDSEEMAGALAATGCAEAPDLESADLIVINSCAIREAAEQKVIGRMGHL
                     ANLRRANPGLRVVLTGCSVREDNAAGLKRRYPEVDLFLRPDEEPELTARLGLAGATAP
                     GQMIGSQRSIGAVQRRSASSAWLPIIYGCDKTCTYCIVPFSRGPERSRPFDDVVAEAR
                     SLADRGYREITLLGQNVNSYGHDLPAESRFGHIRRSRDLGRDIELDGRPDIAELLRAI
                     RDIPRIRFVTSHPWDLSERLIAAMAECPSVCEHLHLPVQSGDDAVLRRMGRQYTIDAY
                     LELVTRLRAAVPGITLTTDVIVGFCGETEAQFERTLDLLRQVRFETVFAAAFSMRPGT
                     PAARLDDDVPRAEKKRRLQALLDLQEGIGRAANEAWLGRTTEVLVDQVRPPSSHDGGE
                     ARVAGRNRNNKLVHFDGGPDLVGQLVNVRVDHAGPYALVGTLDA"
     gene            2374..3330
                     /gene="miaA"
                     /locus_tag="VIF08_01975"
     CDS             2374..3330
                     /gene="miaA"
                     /locus_tag="VIF08_01975"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY8134779.1"
                     /translation="MPWWGRSTPEVERSGAYPPLIVICGATATGKTGVSLELAQSIPG
                     AEIISADSRQVYRGMDIGTAKVTADQRALVPHHGLDLADPDAAFSAADYQRAADSALQ
                     AIAGRGGIALLVGGTGLYLRAVARGMALEETGRDPGVRADLERRLAGEGLHVLVAELR
                     GRAPSLAARTDLANPRRVVRALERVSVAGDAPPPAPRGYPAPSVWIGLQVDPAQHRDW
                     ITARARGQFESGLLHEAAALRERYDPTLPAFSAFGYHEAFAVLDGKLTVEDAVARDAA
                     RTWQFARRQRTWFRAEPDITWFGAPGDPAQVRAQVTAALARR"
     gene            complement(3327..3527)
                     /locus_tag="VIF08_01980"
     CDS             complement(3327..3527)
                     /locus_tag="VIF08_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134780.1"
                     /translation="MDRDEPDVVPPISPGQPPVALGIGVGVYVGRTEEPDSDNDQESH
                     VSPTDNDGETVPLDKARDEVGG"
     gene            3677..4990
                     /gene="hflX"
                     /locus_tag="VIF08_01985"
     CDS             3677..4990
                     /gene="hflX"
                     /locus_tag="VIF08_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY8134781.1"
                     /translation="MPSRSNGRSRMIDLAPPAERAFLVAVDTGKDAGWTAEESLTELA
                     QLAATAGAEVVGAEWQNRRHVDPNWYVGKGKAEDLVSAKRETGFSLLVADDEISPNQQ
                     KALESLLGVKVLDRSGLILDIFAQHARTHEGRLQVELAQLEYQLPRLTRLWTHLSRTQ
                     GGIGSRGPGETQLETDRRIIRDKIKKLKERVENVRRQRETAARSRDRRLMPTVAIVGY
                     TNAGKSSMLNALVGHEAARAEDQLFATLDPTSRNVKLGDGQSVVVTDTVGFIHKLPHQ
                     LVDAFRATLEEVNRADVLLEVVDVADQHAPEHRATVQQVLDDLGAGEKPRLLVYNKAD
                     LVDPAASDAGVAAPSIPGSVLVSAMTGYGMDTLRIRLAAILSDLWEEVDVNLPYSEGE
                     LLSRVRERGTVDIGYRARDVRVRGRVTPTLASELRAAAFGHNGRA"
     gene            5310..5987
                     /locus_tag="VIF08_01990"
     CDS             5310..5987
                     /locus_tag="VIF08_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134782.1"
                     /translation="MPWVEIETEMLALELRSVDLPIEDDQTGDGLSADGSAPRDISPD
                     LDLLRSFGGWAAIEQSHFGPDSFFDCADPLVWCGNNPVPIGGNNLVLIGLQGSDTFDV
                     VTQRDGQLAVCFDRLGANNSPDGSGAFGGCDLVLIADLTGDVMFELVYDGQYQQRNTI
                     GRARIDGDTALFVMPLGTDEAPRYRIVAFERIPPQVAGGEHVDTLPSHPWNVDSFFDI
                     TYSIPGL"
     gene            complement(6045..6377)
                     /locus_tag="VIF08_01995"
     CDS             complement(6045..6377)
                     /locus_tag="VIF08_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134783.1"
                     /translation="MTETGHDRGFGREDAAQQRSPRKDDGDEICAARQRAWKTLSGFS
                     EEKHVGQTALLDARGSPAGSVRHGRRLRVHANNKRSRLAPGAAQHGLSATRSDINHHP
                     LVAGDEGG"
     gene            complement(6577..7362)
                     /locus_tag="VIF08_02000"
     CDS             complement(6577..7362)
                     /locus_tag="VIF08_02000"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEY8134784.1"
                     /translation="MDLSFLSSPETWIALLTLTALEIVLGIDNIVFISIMAGKLPEAQ
                     RGQAYALGLGAAMVMRILLLFSISFIVGLTAPLFTLPFIDREVSGRDLILFGGGLFLI
                     AKATFEIHERLEGSESGAATTRAGATLGRVVLQIMLLDIVFSLDSVITAVGMARELAV
                     MVAAVVIAVLVMLVAARPIGQLVERHPSLKMLALSFLLLIGTALVAEAAHVEIPKGYI
                     YFAMGFSIFVELLNIRAHARSAPVHLRDVYTKEREADSPPGPA"
     gene            7771..7986
                     /locus_tag="VIF08_02005"
     CDS             7771..7986
                     /locus_tag="VIF08_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134785.1"
                     /translation="MLQQLTVTLRRRAAAFMNQGHDGGQNTDSALAHPVLVRQAEIDS
                     LRGVTPAAADVSECTCPEPCERDHANE"
     gene            7994..9226
                     /locus_tag="VIF08_02010"
     CDS             7994..9226
                     /locus_tag="VIF08_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEY8134786.1"
                     /translation="MKATLDRPETVHRSGTVSRAITDPLQADGGGAVSAPALATTPLL
                     PDGYYVPTSRWTRPSTRMRELLATESYLFGPGVYDPMGAQLAMYHGFKAVYFSGYSFA
                     IGHLGTTDMDLYTSVEIADGARRTVSGLRKFQLTMAVGDPDRDEPPRHLEIPPVVVDM
                     DAGYGNIFNVQRTTELYVNAGVAAAHIEDQVLPKRCGHIGGKALVAADEMVGKLRMAR
                     AVANDLGNTDFVIIARTDAISAVDAPESARGMSLAVDRALRYLDTGVPDLVWCEFPTA
                     DRGPVEEWAGAVRRRFPEAAFAFNWSSSFKWYLDPKPMTFDELGDLGFKFIFITLGGQ
                     HATGLGLNELLAAMAERQEQGYIDLQRREWEGKTVIPTRSHHTFSGVPYHHLMGEKYS
                     SARLGRGYVEDLPADKVV"
     gene            complement(9235..9597)
                     /locus_tag="VIF08_02015"
     CDS             complement(9235..9597)
                     /locus_tag="VIF08_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134787.1"
                     /translation="MTTGVSVVRLAMGVVGGIMLLGGLALVASGVPGGFVGAIWLIPS
                     GMVLLIVAFIEVGRYRSQAAEQGGNSAPGPGGGEPAPLEPRFQRTDEVFIDPTTNVTM
                     RVYLDAVTGERRYVAEIR"
     gene            complement(9602..9946)
                     /locus_tag="VIF08_02020"
     CDS             complement(9602..9946)
                     /locus_tag="VIF08_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY8134788.1"
                     /translation="MLIATAPFIAGWKVTETRGHVFGLVVRSRGLGGNIMAGLRSLGG
                     GEIHEYTSLLEDTRRQAIDRMVQNATMMGANAIISFRFDSSELANTMSEIVAYGTAVV
                     VAQDAGAPQPGE"
     gene            9993..>10627
                     /locus_tag="VIF08_02025"
     CDS             9993..>10627
                     /locus_tag="VIF08_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134789.1"
                     /translation="MTPIEWLLDSDPAIRWQAMAALSDAPTAEVVTERAKVAQSGWGA
                     QLLARQESDGVWRWSGWDPQSGQWGGSPGWITMHVLQLLRALGPDPLDPDVASAISKL
                     RDSDAFATSMPEFFAWAGRGFFDGETEACINGRAAAAGAYFHQDVDVIVERLLKEQMA
                     DGGWNCELERGSTRGSFHSTINVLEALLEYERAGTGRHVAQAPEARRRGEE"
BASE COUNT         1724 a         3626 c         3585 g         1692 t
ORIGIN      
        1 cgcttcgccg agcccgtcgt tcagccgggt tggccggcca acaaggtcga cctgcactcg
       61 cactccgacc gatccgacgg cgtcctgccc ccgaccgagc tctaccagca catgaccgag
      121 gtcgggctcg agatctgtgc cctcgccgat cacgacacgc tcgctggcta tcgcctgctg
      181 cgcgacggga tcgccaccgg cgcaatcacc ttgggcccgc gcctgatccc ggcggtcgag
      241 atcaacagcg tcgccgatcg tgcgctgatc gagctgggca ttgagctcga ggagggcgag
      301 ctgcacatcc tgggctacgg cgtcgacacg gacgacggcg tgttcgaggc caagctcgaa
      361 ggccagcgca acgcgcgccg cacgcggctc gagctgatga tcgagcgact gcgcgagctg
      421 ggcttgccga tcgacgagca gatcgcgccg gcgctggcga gcgaggaggc cgttggccgg
      481 ccgcacgtcg cgcgggccat ggtcgccgcg ggtcacgtgg cgagcgtcca acaggcattc
      541 gacgagtgga tcgaccgcaa cggcagggcc tacgtaccgc gccagggcat gcgctcgcgc
      601 gacgcgatcg acgcgatcat ggccgcgggc ggtataccgg tgctcgccca ttacccggcc
      661 gcgcccgagc agccgacact catcgggctg ctcatggagt ggggcgtgcg cggcctcgag
      721 gtgtactacc gccgttacct gccggccact gtcagcgcga tgatcgacct cgcggcgcgc
      781 ctgggtgtcc tggcgaccgg tggctccgac taccacggcg acaccatgtc ctacgctgag
      841 gccacacaga cgacctttgt gccgctcgca gcgggcgagc ggctgctgga ggcaatcgac
      901 actggggtgc gtgccacgtg acgacccggg ccttgccggt gctcgatgtg gtgcggccgg
      961 cgcggcccgc acgcacggat agcgccgcgg ttacgccgag tctggccgag ttcatgaccg
     1021 acgatctggc cctgccgcgc ttccacgtct ggacactggg ctgtcagatg aatcactccg
     1081 attcagaaga gatggccggc gcgctggccg ccacgggctg tgcagaagcg cccgatctcg
     1141 agtcggccga cctgatcgtg atcaactcgt gcgccatccg cgaggcggcg gagcagaagg
     1201 tgatcggtcg gatgggccat ttggccaacc tgcgccgcgc caatccgggg ctgcgcgtcg
     1261 tgctgaccgg gtgctcggtc cgcgaagaca acgcggccgg cttgaagcgc cgctatccgg
     1321 aggtcgacct gttcctgcgc ccggacgagg agcccgagct gaccgcgcgg ctgggcctgg
     1381 ccggagctac tgcacccggg cagatgatcg gctcccagcg ttcaatcggt gccgtccagc
     1441 gccgcagcgc gagcagcgcc tggctgccga tcatctacgg ctgcgacaag acgtgcacct
     1501 attgcatcgt gcccttctca cgtggcccag agcggtcgcg cccgttcgat gatgtcgtgg
     1561 cggaggcgcg ctcgctcgcc gaccggggct atcgcgagat cacgctgctc ggccagaacg
     1621 tcaactccta cggccacgac ctgcccgctg agtcgcgctt tggccacatc cgccgctcgc
     1681 gcgacctggg ccgcgacatc gagctcgacg ggcggccgga tattgccgag cttctgagag
     1741 caatccgcga catcccgcgc atcaggttcg tcacctcgca tccgtgggat ctgtcggagc
     1801 gcctcattgc ggcgatggcc gagtgccctt ccgtgtgcga gcaccttcat ctgccggtcc
     1861 agtcgggcga cgacgcggtg ctgcgccgga tgggccgcca atacacgatt gacgcgtacc
     1921 tcgagctggt cacgcgactg cgcgccgccg tgcccgggat cacgctcacg acggacgtga
     1981 tcgtgggctt ctgtggggag acggaagcgc agttcgagcg caccctggac ctgctccgcc
     2041 aggtgcgctt cgagacggtg ttcgcggccg cgttctccat gcggcccggc acgccggcgg
     2101 cgcgcctcga cgacgacgtg ccgcgcgcgg agaagaagcg ccgcctccag gcgctgctcg
     2161 acctccagga ggggatcggc cgcgcggcga acgaagcgtg gctgggcagg acgaccgagg
     2221 tgctcgtcga ccaggtgcgg ccaccgtcgt cgcacgacgg cggcgaggca cgcgtcgccg
     2281 ggcgcaaccg caacaacaag ctggtccatt tcgacggcgg accggatctt gttggtcagc
     2341 tcgtcaacgt gcgggtcgac cacgccggcc cgtatgccct ggtggggacg ctcgacgcct
     2401 gaggttgagc ggtcgggcgc ctacccgccg ctgatcgtca tctgcggcgc gaccgcgacc
     2461 ggcaagaccg gggtgtcgct ggagctcgcg caatcgatcc cgggcgcgga gatcatcagc
     2521 gccgattcgc gccaggttta ccgcggcatg gatatcggca cggccaaggt gaccgccgac
     2581 cagcgcgccc tggtgccgca ccacgggctc gatttggccg atcccgatgc ggccttctca
     2641 gcagccgatt accaacgggc cgcagactcc gccctgcagg ctattgcggg ccggggcggg
     2701 atagcgctgc tcgtcggtgg gaccggtctg tacctgcgag cggtcgcccg gggaatggcg
     2761 ctcgaggaga cgggacgtga tcccggcgtt cgcgcggacc ttgagcgccg cctcgcgggg
     2821 gagggcctgc acgtgctcgt cgcagaattg cgcggccgtg ctcccagcct tgcggcgcgg
     2881 acagaccttg ccaatccgcg gcgagtggtg cgtgccctgg agcgggtgtc agttgccggg
     2941 gacgcgccgc caccggcgcc gcgcggctac ccggctccgt cagtgtggat cggcctgcag
     3001 gttgatcccg cgcagcaccg cgactggatc accgcgcgcg cccggggcca gttcgaatca
     3061 ggcctgctcc acgaggcggc cgcgctgcgg gaacgctacg acccgaccct gcccgcgttc
     3121 agcgcgttcg gctaccacga ggctttcgcc gtgctggatg gcaagctcac cgtggaggac
     3181 gccgttgccc gcgatgcggc ccgcacgtgg cagttcgcgc gtcgccagcg cacctggttc
     3241 agagccgagc cagacatcac atggttcggc gcacccggcg atcccgccca ggtgcgcgct
     3301 caggtcacag cggcgctggc tcgacgctag cccccaacct cgtccctcgc cttgtccagc
     3361 gggaccgtct cgccatcgtt atcggtcgga gagacatggc tctcctggtc gttgtcggag
     3421 tccggctcct cggtccgtcc gacgtatacc ccgacgccta tgcccagcgc cacagggggc
     3481 tgtccgggcg agatcggcgg gacaacatcg ggctcgtcgc gatccatgat ctgcctcccg
     3541 gctatcggca ttcgaggcgc ttgggccgcg cctgcgttca ggttgcagcg cgcggcattg
     3601 caaacgcgcg cgtcagagca gccgcgccat caatccccag ccccgaagcg tcgagcggca
     3661 gccgtatcct gtcggcatgc ccagtcgctc caatggccgc tcgcgcatga tcgatctcgc
     3721 gccgccggcc gagcgcgcct tcctggtcgc ggtcgatacg ggcaaggacg ccggctggac
     3781 cgctgaggag tcgctgacgg agctggctca gctggccgcg acggctgggg ccgaggtggt
     3841 cggtgccgaa tggcagaacc ggcgccacgt ggatcccaac tggtacgtgg gcaagggcaa
     3901 ggcggaggat ctcgtgtcgg ccaaacgcga gacagggttt tcgctgctcg tcgccgacga
     3961 cgagatctcg cccaatcaac agaaggcgct cgagagcctg ctcggggtca aggtgctcga
     4021 tcggagcggt ctgatcctcg acatcttcgc ccagcacgcg cggacgcacg aaggccgtct
     4081 ccaggtcgag ctggcgcagc tcgagtacca gctgcccagg ctgacccgcc tctggaccca
     4141 cttgagccgt acccagggcg gcatcggctc gcgcgggccc ggcgagaccc agctcgagac
     4201 agaccggcgg atcatccgcg acaagatcaa gaagctcaag gaacgcgtcg agaacgtgcg
     4261 ccgccagcgc gagacagcag cacgctcgcg cgaccggcgg ctgatgccaa cggtcgcgat
     4321 cgtcggctat accaacgccg gcaagagctc gatgctcaac gcgctcgttg gtcacgaggc
     4381 cgcccgcgcg gaggaccagt tattcgccac gctcgacccg acgagccgca acgtcaagct
     4441 gggcgacggc cagagcgtgg tcgtgaccga cacggtgggc ttcatccaca agctgccgca
     4501 tcagctcgtc gacgccttcc gcgcgaccct cgaagaggtc aaccgcgccg acgtgctgct
     4561 cgaagttgtc gatgtcgcgg accagcacgc gccggagcac cgcgcaaccg tgcagcaggt
     4621 cctcgacgat ctgggcgctg gtgagaagcc gcgcctgctc gtctacaaca aggccgacct
     4681 ggtcgaccca gcggcgagcg acgccggcgt ggccgcgccc agcatccccg gcagcgtgct
     4741 cgtctcggcg atgaccggct atggcatgga cacgctacgc atccgcctgg ccgcgatcct
     4801 gtcggatctc tgggaagagg tcgatgtcaa tctgccctac agcgaaggcg agctgctgtc
     4861 gcgcgtccgc gagcgcggca ccgtggacat tggctatcgc gcgcgcgacg tgcgggtgcg
     4921 cggccgggtc acgccgacgc tcgcgagcga gctccgagcc gccgcctttg gccacaacgg
     4981 gcgggcgtag caagccgcgg cgcatttgac aacgcgccgg acaatgaatg caggcggcgc
     5041 tgcgatcgcc ttacggggga ccgcgaccca ggtgaggtga tccgatgagt cgtcacgcca
     5101 gtgcgtcggg agtcctgatt gcactcacag tcatcgctat cggatgctcc ggcgttaccg
     5161 gctcgaactc accgccaact ccaaccccga caccttcccc gacctattac gcgccggtgc
     5221 ccccgacacc cacaccgacg gcttctccaa ccccaccacc gaccgccagc cccagtccgg
     5281 cgccgacggt gcgtccagac ccgactccga tgccctgggt cgagatcgag acggagatgc
     5341 tcgcgcttga gttacgcagc gtggatctgc cgattgagga cgaccagact ggcgacggac
     5401 tcagtgctga cggcagcgcg ccgcgcgaca tctcgcccga tctcgatttg ctccggtcct
     5461 ttggcggctg ggccgcgatt gagcagtcgc acttcgggcc ggacagcttc tttgactgtg
     5521 ccgatccgct cgtgtggtgc ggcaacaacc cggtccccat cggcggcaac aatcttgtcc
     5581 tgatcggcct gcagggcagc gacaccttcg atgtcgtcac gcaacgtgac ggtcagctgg
     5641 cagtctgctt cgatcgcctg ggcgccaaca actcgcccga cggcagcggc gcgttcggcg
     5701 gctgcgacct ggtgctgatc gccgatctca ccggcgacgt gatgttcgag ctggtctacg
     5761 acggtcagta ccagcagcgc aacacgatcg gccgggcgag gatcgacggt gatacggcgc
     5821 tgttcgtcat gccgctcggc acggatgagg cgccgcgcta ccggatcgtt gccttcgagc
     5881 ggatcccgcc acaggttgcc ggtggcgagc acgtcgacac gcttcccagc catccatgga
     5941 acgtcgattc cttcttcgac atcacctact cgatcccggg tttgtaggat gtccgcatgc
     6001 gccaggtcat cggccgccgc cttctcgagg tagacgtcgg ttagttaacc gccctcatcg
     6061 cccgccacga gcgggtggtg attgatatcg gaacgggtgg cggacaggcc gtgctgcgcc
     6121 gcgcccggcg cgagccgcga acgcttgttg ttggcgtgga cgcggaggcg acggccatgg
     6181 cggacgcttc ccgcagggct gccgcgagcg tcaagaaggg cggtctgccc aacgtgcttt
     6241 tcttcgctga agccgctgag cgtcttccag gcgcgctggc gggccgcgca gatctcgtca
     6301 ccgtcgtcct tccgtgggga tcgctgctgc gcggcatcct cgcggccgaa accacgatcg
     6361 tggccggtct cagtcacctg atgagtccca ccggcgagct ggtgctgttc ctgtcggaga
     6421 tcgatcgggc gcaaaccaat gcgctggtgg atcggctcga cggtcttgag tccgtcgaag
     6481 tgcgcgaagc gacagagtcg gacgttgccg agctgtcgtc tggttgggcc aggcgtttgg
     6541 gtatcccggg cagccggccg gcctgggtga tcagactcag gcggggccag gtggtgaatc
     6601 ggcctcgcgt tccttggtgt aaacgtcgcg caggtgcacc ggtgccgagc gcgcgtgggc
     6661 gcggatgttg agcagctcga cgaagatcga gaagcccatc gcgaagtaga tgtagccctt
     6721 ggggatctcg acgtgcgcgg cctctgctac aagcgccgtg ccgatcagta acaggaagct
     6781 cagcgccagc atcttgagac tggggtggcg ctcgacgagc tggccgatcg gccgggcagc
     6841 gacgagcatc acgagcacgg cgatgacgac ggccgcgacc atgaccgcga gctcgcgcgc
     6901 catgcccact gcggtgatga ccgagtcgag cgagaacacg atgtcgagca gcatgatctg
     6961 tagcaccacg cggccgaggg tcgcgccggc gcgcgtcgtt gccgcgccgc tctccgatcc
     7021 ctcgaggcgc tcgtggatct cgaacgtcgc cttggcgatc aggaacaggc caccgccaaa
     7081 gaggatcagg tcgcgcccgc tcacctcacg atcgatgaat ggcaaggtga agagcggcgc
     7141 tgtgagaccg acgatgaacg agatggagaa cagcagcagg atgcgcatga ccatggctgc
     7201 gcccaagccc aacgcatagg cctggccgcg ctgcgcttcc ggcagcttgc cggccatgat
     7261 cgagatgaac acgatgttgt cgatgcccag cacgatctcg agcgcggtca gcgtcagcag
     7321 cgcaatccac gtctcagggc tggacaggaa gctcagatcc acggcccgca aaggatatct
     7381 ggagcgattg tggctcctcg cgacctgggc actaaatgga agaggagggg agccccgcgg
     7441 ccaccctcct cctggttttc tctggggcgc agcgccccgt tgactacttg ccggctccgc
     7501 ccttggcctt cttggtcgag gtcttgggct tcttgccgcc gcccttgctg ccaggtccct
     7561 tgtcgcccat cgtcaagccc cttctctacg gtgcaaaacg cgcattccgc gcgtgatctc
     7621 aatcgtaaac agcggctgca aacgtttcat gtcgcacgtg cacgttgcag agtgcctgcc
     7681 gcaagactga cgttacccgc cacgcagcgc gtcgtaacag gtctggaggg tcgacgcgcc
     7741 tacgctgctg atccacaagg aggtggacgc gtgcttcagc agctgactgt caccttgcgc
     7801 agacgcgcgg ccgcattcat gaatcaaggc cacgatggtg gccagaacac cgactctgca
     7861 ctcgcacacc ccgtcctggt gcgacaggcc gagatcgaca gcctgcgcgg cgtgacgccc
     7921 gcggcggccg acgtgagcga atgcacatgt ccggagccgt gcgagcgcga tcacgccaac
     7981 gagtgatgag ccggtgaagg ccaccctgga ccgaccggag actgtccacc gatcgggcac
     8041 agtcagccgc gccatcacgg accctcttca ggctgatggc ggcggggcgg tcagcgcccc
     8101 agcactggcc acgaccccgc ttctgccgga cggctactac gtccccacgt cgcgctggac
     8161 gcgccccagc acgcgcatgc gcgagttgct cgcgactgag tcatacctgt tcggacctgg
     8221 cgtctacgac ccgatgggtg cgcagctcgc gatgtaccac ggcttcaagg ccgtgtactt
     8281 cagcggctat tccttcgcga tcggtcatct cggcacgacc gacatggatc tctacacgag
     8341 cgtcgagatt gccgatggcg cccgacgcac cgtgagtggc ctgcgcaagt tccagctgac
     8401 gatggcggtt ggcgaccccg accgcgacga gccaccccgg cacctggaga tcccgccggt
     8461 ggtcgtggac atggacgccg ggtacggcaa catcttcaac gtccagcgca cgaccgagct
     8521 gtacgtcaac gccggcgtcg cagcggcgca catcgaggac caggtactac ccaagcgctg
     8581 cggccacatt gggggcaagg cgctggtggc cgcggacgag atggtgggca agctacgcat
     8641 ggcgcgcgcg gtggcgaacg acctgggcaa cacggacttc gtgatcatcg cccgcaccga
     8701 cgccatctca gccgtcgacg cgcccgagtc ggcgcgaggc atgagtcttg ccgtcgaccg
     8761 tgccttgcgc tatctcgaca ccggtgtccc ggacctggtg tggtgtgagt tcccgacggc
     8821 tgatcgggga cccgtggaag agtgggcagg ggcggtccgt cggcggtttc cagaggcggc
     8881 cttcgccttc aactggtctt cgtcgttcaa gtggtacctc gacccgaagc cgatgacctt
     8941 cgatgagctg ggtgacctgg ggttcaagtt catcttcatc acgctcggcg gccagcacgc
     9001 gaccggcctg ggcctgaacg agctcctcgc cgcgatggcg gagcgccagg aacagggcta
     9061 catcgatctt cagcggcgcg agtgggaagg caagaccgtc atcccgacgc gcagccacca
     9121 cacgttcagc ggcgtgcctt atcaccacct gatgggtgag aagtactcgt ccgcccgcct
     9181 gggccgggga tacgtcgagg acctgcccgc agacaaggtc gtctagctcc gcgtctagcg
     9241 gatctccgcc acgtacctcc gctcgccggt caccgcgtcg aggtagacgc gcatcgtcac
     9301 gttcgtcgtg gggtcgatga agacctcgtc ggttcgctgg aatcgcggct cgaggggcgc
     9361 cggctcgccg ccgcccggcc cgggagccga attgccgccc tgttcggcag cttgtgagcg
     9421 atagcgaccg acctcgatga acgccacgat caagagaacc atgccgcttg ggatgagcca
     9481 gatggccccg acgaagccac ccggcacgcc actcgcaacg agcgcgagtc cgcccagcag
     9541 catgatcccg ccgaccacgc ccattgcgag gcgtacgacg gacacgcccg tggtcatccc
     9601 gctactcgcc gggctgcggc gcgccggcgt cctgggcgac cacgacggcc gttccatagg
     9661 cgacgatctc cgacatcgtg ttcgcgagct cggagctgtc gaagcggaac gagatgatcg
     9721 cgttggcgcc catcatcgtg gcgttctgga ccatgcggtc gatcgcctgg cggcgtgtgt
     9781 cctcgagcag cgacgtgtac tcgtggattt cgccgccgcc cagggaccgt agcccggcca
     9841 tgatgttgcc gcccagtccg cggctgcgga cgaccaggcc aaagacgtgg ccgcgggtct
     9901 ccgtgacctt ccagccggcg atgaacggtg cggttgcgat gagcatgtga tcgagacctc
     9961 cttgtgtcac gttagagtct aggcttccgc agatgacacc gatcgaatgg ctgctggact
    10021 ccgacccggc catccgctgg caggcgatgg ccgctctcag cgacgcgccc accgcggagg
    10081 tagtcacaga gcgcgcaaag gtcgcccagt ccggctgggg cgcgcagctg ttggcgcgcc
    10141 aggagtccga cggcgtatgg cgctggtcgg gctgggaccc ccagtccggg cagtggggcg
    10201 gatcgcccgg ctggatcacc atgcacgtcc tccagctgct ccgggcactc ggtccggatc
    10261 cgctcgatcc agacgtcgcc agcgcgatct ccaagctgcg cgacagcgac gcattcgcga
    10321 cctcgatgcc cgagttcttc gcatgggccg gtcgtggttt cttcgacggc gagaccgagg
    10381 catgcatcaa cggccgcgcc gccgcggcag gcgcctattt ccaccaggat gtcgacgtta
    10441 tcgtcgaacg gctcctcaaa gagcagatgg cggacggcgg ctggaactgc gagctggagc
    10501 gcgggtcgac gcgcggctct ttccattcaa cgatcaacgt cctagaggcg ctgctggagt
    10561 acgagcgcgc cggcactggc aggcacgtgg cccaggcccc ggaggcgcgc cggcgcggcg
    10621 aagagta
//
LOCUS       DATBRX010000057         5469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1135544, whole genome shotgun sequence.
ACCESSION   DATBRX010000057 DATBRX010000000
VERSION     DATBRX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135544"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VIF08_02030"
     CDS             <1..204
                     /locus_tag="VIF08_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134790.1"
                     /translation="AERTNQALATGADAVATACPFCLVMMRDGVADSGPRGEGVAVQD
                     IAEIFAAGLPAPGTNGRSLPVVQ"
     gene            394..1548
                     /locus_tag="VIF08_02035"
     CDS             394..1548
                     /locus_tag="VIF08_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8134791.1"
                     /translation="MTVLNEEQQALRDAVRELAQDKIAPRAGEIDASGKFPHDIVELL
                     AQHDILALPFPTEYGGLGGDMVSILVVIEEISRIDASCGLIPAVQELGSLPLVHAGTE
                     EQKKRWVPDLAAGKILSAFALTEAGAGSDAANARTSAKRDGDGWILNGEKRFISQGDV
                     ADIVAVFAVTDSTPEAVKSHRHMTCFYVEKGTLGYSSPRVEHKMGIRGSTTAELAFQN
                     ATVPDENRVGEVGDGWGLAMKTFERSRPGIGAQAVGIAQGAMDVAVKYASERKQFGKP
                     IGAHQMIGAMLADMATRTEAARQLLWTAAEHIERGDPHAARWASMCKLFAGDTAMAVT
                     TDAVQVLGGYGYTTEYPVERMMRDAKITQLYEGTQQIQRLIIARDLLASL"
     gene            1563..2339
                     /locus_tag="VIF08_02040"
     CDS             1563..2339
                     /locus_tag="VIF08_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY8134792.1"
                     /translation="MNIVVLLKAVPVVGTERLDSGRLTDRSVQLEANGNDEYLLEKAL
                     KLAETHGGEVVTLTVGPAGSTEPIRKALAMGTARAVHVADEALRGSDMRATLDVLAAA
                     LKKMQPWDLVFAGADTSDGQGGVLGAALAVRLALPYLSYASEIEPSDGGVRIHRLNPT
                     GYDILEAATPAVVMGTQLLGAPRYPSLRGIMQARQKPVETWSLADLGLEAGGVGSGAA
                     TTKTLDAAKPPERAGATFVREAPDDAVAMVVEYLAARRLL"
     gene            2339..3322
                     /locus_tag="VIF08_02045"
     CDS             2339..3322
                     /locus_tag="VIF08_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY8134793.1"
                     /translation="MATQIWAIGEIADGQPTKLTLELATLARTLAAAGGGQARTVLVG
                     AGAAAAAAGVTAYGPDVLAVEAETAGLPVATVIAPRVAALVAERKPDFLLIGASNDGK
                     DVAGLLLGLTDLPLLANGSGVDWGDGAPRVEMSTFGGKLVTHSTFAGTRGMVIVRPAS
                     VAAEAASAPGKVEQVSVTVAEELSPVRVTGSVEESGAAAPIEEARVIVGAGRGIGSPD
                     GLKVIEDLAAALGGAVGATRAVVDSGWVPYGQQIGQTGKIVKPALYLACGISGAIQHK
                     VGVQTASTIIAINRDADAPIAEFADLFVVGDLFEIVPRLAAAIRARKSGSA"
     gene            complement(3327..4358)
                     /locus_tag="VIF08_02050"
     CDS             complement(3327..4358)
                     /locus_tag="VIF08_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134794.1"
                     /translation="MRYRLVERVTQAVTVLLLVGGCGWALITWGPGLACQNLSLFCPP
                     APGAQELGPFVNDYMAAAGSDTDAEEYTLFLKSEKPLTVAPYRVGLIAAVNVADHAID
                     GLLTLALPSEIVATRPADVGTVIWVRWGETHEGTYHDEGTRRVSGEAYRGYCAIVVID
                     LAAGEIVGQAFYEAVSPPTTTTGSGDVHTQVNVESVANWVKTLPVHAAGSTPVPSSRA
                     TPTNSPSRSDSGTALEPGESLTLSGDQWGSGDMISEEQFELLAGDYSVVFEGEASKGC
                     ANCMAIKVFNERDEYPIFVVNDPDRIAEGRWRFTGVFTLEWWEDGATFHFELDAPKGS
                     WTITLTRDS"
     gene            4517..4819
                     /locus_tag="VIF08_02055"
     CDS             4517..4819
                     /locus_tag="VIF08_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY8134795.1"
                     /translation="MTYVIAEPCVDVMELSCVAVCPVDCIHYDKGVDRMLYIDPNECI
                     DCGACEPECSVEAIRVEDQLPADWTPYTEINALWFTDKQAARAKLDALKPRAAAAG"
     gene            4859..>5469
                     /locus_tag="VIF08_02060"
     CDS             4859..>5469
                     /locus_tag="VIF08_02060"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY8134796.1"
                     /translation="MTEAAAQLKPIEVEGHTYERVGVRTRMVKFGDDLDALVREYVTP
                     LAQPGDWIALSEKVVSVCQNNVRHISTVKVGRLARLIVKGVKKYPEDIAWEHPAKMQV
                     AIDMAGYPRMLTALVVGGLTRLVGIHGVFWRIAGRVSEIDGFNPRAMWPYTEYAILPP
                     KHPDGLVQRMEDQLGIPNTIIDGNNIDVKIISCSRKLPVDAATA"
BASE COUNT          936 a         1848 c         1843 g          842 t
ORIGIN      
        1 gccgagcgca ccaaccaggc gctcgccacc ggcgccgacg cggtcgcgac ggcatgcccg
       61 ttctgcctgg tgatgatgcg cgacggcgtc gccgattccg gcccccgcgg cgagggcgtc
      121 gcggtccagg acatcgccga gatcttcgcc gcgggcctgc ccgcgcccgg caccaacggt
      181 cgctcgctgc ccgtcgtcca gtagccgggg agcgcgaccg gtgatccgcg ctggcgggct
      241 tgatgtaccg acccgactag atatgagccg acccgaccag atatgaacca ggccgactgg
      301 aaatgcaccg gtgcgcatgg ttagtcgggt cgatacatat tcaatcgggt cggtacatga
      361 cctcgtttct ggcaagtcgg tagcatcggg ctcatgaccg ttctcaacga agagcagcag
      421 gcgctgcgtg acgccgtgcg tgagctggcg caggacaaga tcgcgccgcg cgccggcgag
      481 atcgacgcga gcggcaagtt cccgcacgac atcgtcgagc tgctcgccca gcacgacatc
      541 ctggccctgc cgttcccgac cgagtacggc ggcctcggcg gcgacatggt ctcgatcctc
      601 gtggtgatcg aggagatcag tcgcatcgac gcgagctgcg ggctcatccc agccgtccag
      661 gagctgggct cgctgccgct cgtccacgcc ggcacggagg agcagaagaa gcgctgggtg
      721 cccgacttgg cggccggcaa gatcctcagt gcgtttgcgc tgacggaggc gggcgccgga
      781 tcggacgcgg cgaacgcgcg gacgagtgcc aaacgcgacg gcgacggctg gatcctcaac
      841 ggcgagaagc gcttcatcag ccagggcgat gtcgccgaca tcgtggccgt attcgccgtc
      901 accgactcga caccggaggc cgtcaaatcg caccgccaca tgacctgctt ctacgtggag
      961 aagggcacgc tcggttactc atcgccaagg gtggagcaca agatgggcat ccgcggctca
     1021 accactgccg agcttgcctt ccagaacgcc accgtgccgg atgagaaccg cgtcggcgag
     1081 gtcggcgacg gctggggcct ggcgatgaag acgttcgagc gcagccggcc gggaatcggt
     1141 gcacaggcag tcggcatcgc ccagggcgcg atggacgtcg ccgtcaagta cgcgagcgag
     1201 cgcaagcagt tcggcaagcc aattggcgcg caccagatga tcggcgcgat gctggccgac
     1261 atggccacgc gcacagaagc cgcgcgccag ctcctgtgga ccgcggcgga gcacatcgag
     1321 cgtggcgatc cccatgctgc gcgctgggcg agcatgtgca agctgttcgc gggtgacacc
     1381 gcgatggcgg tcaccacgga cgcggtccaa gtgctcggcg gctacggcta cacgaccgag
     1441 tacccggtcg agcggatgat gcgcgacgcc aagatcacgc agctctacga gggcacacag
     1501 cagatccagc gactgatcat cgcccgcgat cttctggcca gcctgtaacc tccgctctgc
     1561 ccatgaacat cgtcgtcctt ctaaaggccg tgcccgtggt cggcaccgaa aggctcgatt
     1621 cgggccggct gaccgatcga tcggtccagc tcgaagcgaa cggcaacgac gagtacctgc
     1681 tcgagaaggc gctcaagctg gccgagacgc atggcggcga ggtcgtgacg ctgaccgttg
     1741 gcccggccgg gtcgaccgag cccatccgca aggcgctcgc gatgggcacc gctcgcgccg
     1801 tccacgtcgc cgacgaggcg ctgcgcggct cggatatgcg tgccacgctc gacgtgctcg
     1861 ccgccgcgct caagaagatg cagccgtggg acctcgtgtt cgcgggggcc gacacgtccg
     1921 atggccaggg tggggtgctc ggcgcggcgc tggccgttcg actcgcgctg ccgtacctgt
     1981 cctacgcgag cgagatcgag ccgagtgatg gcggggtgcg gatccaccga ctgaacccca
     2041 ccggctacga catcctcgag gcagcgacgc cggccgtggt catgggcacg cagctgttgg
     2101 gtgcgccgcg ctacccgtcg ttgcgcggca tcatgcaggc gcgccagaag ccggtcgaga
     2161 cttggtccct cgctgacctg ggcctggagg caggaggcgt tggctccgga gcggccacga
     2221 ccaagacgct cgatgcggct aagccaccgg agcgggccgg cgcgacgttc gtgcgcgagg
     2281 cgcccgacga cgccgtggcg atggtcgtcg agtacctcgc tgcgcggagg ctcctctgat
     2341 ggccacacag atctgggcga tcggcgagat tgccgacggc cagccgacca agctgacgct
     2401 cgagctggcg acactggctc gcacactcgc ggccgcgggt gggggacagg cgcgcaccgt
     2461 cctcgtgggc gcgggcgccg cggccgccgc agccggcgtg actgcctacg gtccggacgt
     2521 gctcgcagtc gaagcggaaa cggccggtct gcctgtcgca acggtgatcg ccccccgggt
     2581 agcagcgctg gtggccgaaa ggaagccaga cttcctgttg atcggtgcct ccaacgacgg
     2641 caaggatgtc gccggcctgc tcctgggcct gaccgacctg cccttgctcg ccaacggctc
     2701 cggcgtggac tggggcgacg gcgcgccgcg tgtcgagatg agcaccttcg gcggcaagct
     2761 ggtgacgcac agcaccttcg ccggcacgcg cggcatggtc atcgtccggc cggcttcggt
     2821 cgccgcggaa gcggcgagcg cgccgggcaa ggtcgagcag gtcagcgtga ccgtggccga
     2881 ggagctatcc ccagtgcgag tcaccggctc cgtcgaggag tcgggtgctg ccgcgcccat
     2941 cgaggaggcg cgcgtcatcg tcggcgccgg gcgcggcatt ggctcacccg acggcctgaa
     3001 ggtgatcgaa gacctggccg cggcgctcgg tggtgccgtg ggcgcgacga gagcagtcgt
     3061 cgattccggt tgggtgccgt acggccagca gattggccag accggcaaga tcgtcaagcc
     3121 cgcgctgtac ctcgcgtgcg gcatcagcgg cgccatccag cacaaggtcg gcgtccagac
     3181 cgcgagcacg atcatcgcca tcaaccgcga cgccgacgcc cccatcgccg agtttgccga
     3241 tctcttcgtg gtcggcgacc tgttcgagat cgttcccagg ctggcagccg ccatccgcgc
     3301 ccgcaaatcg gggagcgcct gacctttcag gaatcgcgcg tcagcgtgat cgtccagctg
     3361 cccttgggcg cgtccagctc gaagtggaag gtcgcgccgt cctcccacca ttcgagcgtg
     3421 aacacgccgg tgaaccgcca gcggccttcg gcaattcgat ccgggtcgtt gaccacaaag
     3481 atcgggtact catcgcgctc attgaagacc ttgatggcca tgcagttcgc acatcccttg
     3541 ctcgcctcgc cctcgaacac gacgctgtag tcaccggcga gcagctcgaa ctgctcctcc
     3601 gaaatcatgt cgccagaccc ccactgatcg ccgctgagcg tcaacgattc accaggctcc
     3661 aacgccgtcc cgctgtcgct ccgactgggt gagttcgttg gcgtcgcgcg cgacgatggc
     3721 accggagttg acccggccgc atgcacaggt agagtcttta cccagttcgc gacgctttcg
     3781 acgttcacct gcgtgtggac gtcgccagaa ccggtcgttg tggtgggtgg gctcacggcc
     3841 tcgtagaacg cttgacccac gatctctccg gcggcgaggt cgatgacaac aatcgcgcag
     3901 taaccgcggt aggcttctcc gctcacccgc cgtgtgccct cgtcgtgata ggtgccctcg
     3961 tgggtctcgc cccagcgcac ccagatcacc gtacccacgt cggccggccg cgtcgccacg
     4021 atctcggacg gcagcgcgag ggtgagcagg ccgtcgatcg cgtgatcagc cacgttgaca
     4081 gccgcgatca ggcccactcg gtacggcgcc acggtaagcg gcttctcgga tttgagaaag
     4141 agcgtgtatt cctcggcgtc ggtgtcggat ccggcggccg ccatgtagtc gttcacaaac
     4201 ggacccagct cctgggcgcc cggagccggt gggcagaaca ggctgaggtt ctggcatgca
     4261 aggcctggtc cccaggtgat gagcgcccag ccgcagccac ccacgagcag caggaccgtc
     4321 accgcttggg tgacgcgctc aacaagccga tagcgcacgc caggagacct ccggggtcct
     4381 ggcgccagca tccactgcag ccggcacgcg gccaagtgcc gaaaggcccc tcccaagaat
     4441 ggtccatctg accctagccc gcgtgcggct caggccggac catcgggtcg ctgaccaact
     4501 ctcagagagg ggcaaagtga cctacgtaat cgccgaaccg tgcgtcgacg tgatggagct
     4561 ctcgtgcgtg gccgtctgcc ccgtcgactg catccactac gacaagggtg tggatcggat
     4621 gctctacatc gaccccaacg agtgcatcga ctgcggcgca tgtgagcccg agtgctccgt
     4681 cgaggccatc cgcgtggagg accagctgcc ggccgattgg acgccctaca cagagatcaa
     4741 cgcgctctgg ttcaccgaca agcaggccgc gcgagcgaag ctcgacgcgc tcaagccgcg
     4801 ggccgccgca gccggctagc cgggcgggaa actctatggg ccgttaggct ccgcgcctgt
     4861 gacagaggca gcggcccagc tcaagcccat cgaggttgag gggcacacat acgagcgtgt
     4921 gggcgtgcgc acgcgcatgg tcaagttcgg cgatgacctg gacgcgctcg tacgcgagta
     4981 cgtcaccccg ctggcacagc cgggtgactg gatcgcgctc tctgagaagg tcgtctccgt
     5041 ctgccagaac aacgtgcgcc acatctctac ggtcaaggtc ggtcggctcg cgcgactgat
     5101 cgtcaagggc gtcaagaagt accccgagga catcgcctgg gagcatccgg ccaagatgca
     5161 ggtggccata gacatggccg gctatccgcg catgctgacg gcgctagtgg tcggtggcct
     5221 gacccggctg gtcggcatcc acggcgtctt ctggcgcatc gccggccgcg tttctgagat
     5281 tgacggcttc aatccgcgtg cgatgtggcc ctacaccgag tacgcgatcc tgcccccgaa
     5341 acacccggat ggtctcgtgc agcgcatgga ggatcagctg ggcatcccga acacgatcat
     5401 cgatggcaac aacatcgacg tgaagatcat ctcgtgcagc cggaagctgc ctgtcgacgc
     5461 tgccacggc
//
LOCUS       DATBRX010000058         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3258471, whole genome shotgun sequence.
ACCESSION   DATBRX010000058 DATBRX010000000
VERSION     DATBRX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3258471"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..817
                     /gene="phoU"
                     /locus_tag="VIF08_02065"
     CDS             71..817
                     /gene="phoU"
                     /locus_tag="VIF08_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY8134797.1"
                     /translation="MSDQNTGASEAREATAAAESEGLPPRPQYYPSREMLDRELREIK
                     DEVLRMGSLVAVQIGDAIDALVKHDTDAATVAIASDGRTNEAQRHIAQLIVTTIATQA
                     PVARDLRYLLALDHVTYELERMGDHAASVAKQARKLAPEPPLKDYVDLPAMGTLAAEE
                     VRGVLRALVDLDVDLARRVAAGDNEIDALYHQIFSEVLELMRQDPTNVDRGTRILFAA
                     KNLERIGDRVTNIAEDVVFLATGDIEDLNP"
     gene            832..1263
                     /locus_tag="VIF08_02070"
     CDS             832..1263
                     /locus_tag="VIF08_02070"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY8134798.1"
                     /translation="MARPDPQRVLFLCVHNSARSQMAEGLLRARAGDRFEAFSAGNEA
                     TEVRPLAIAAMAEIGIDISGQRSKTTADLGGQRFDYVITVCDDAKEACPYYANAAHQI
                     HWGFDDPSAATGSDDERLAVFRRVRDEIAARIDEFVSAAGA"
     gene            1283..1795
                     /locus_tag="VIF08_02075"
     CDS             1283..1795
                     /locus_tag="VIF08_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8134799.1"
                     /translation="MDAGPDGTDFALYLIRHAHAGNAANWEGDDAERPLSARGKEQAQ
                     KLGRLLAARGVVPDAIVSSPKVRARQTANVVADALGVGVASDDRLAEMFDLDALAAIM
                     AGVGGTSLMVVGHDPDFSEMLATLVGAAELPMRKGAIARVDVALPLGPGCGILRWLIP
                     PELLPDQADD"
BASE COUNT          355 a          705 c          707 g          287 t
ORIGIN      
        1 gtgttcacaa acccgcagaa ccgactgacc gaagactatg tctccggtcg cttcggttag
       61 gaggccagcc atgagcgacc agaacaccgg cgcgagcgag gcccgcgaag ctacagcggc
      121 ggcggagtcc gagggcctgc caccgcgacc gcagtactac cccagccgcg agatgctcga
      181 ccgcgagctg cgcgagatca aggacgaggt gctgcgcatg ggcagcctcg tcgcggtcca
      241 gatcggcgat gcgatcgatg cgctggtcaa gcacgacacc gacgcggcca cggtggcgat
      301 cgccagcgat ggacgaacca acgaggcgca gcgccatatc gcgcagctga tcgtgaccac
      361 gatcgcgacc caagctccgg tcgcgcgcga cctgcgctac ctcctcgcgc tcgaccatgt
      421 cacgtacgag ctcgagcgca tgggcgacca tgctgcgtcc gtggccaagc aggcgcgcaa
      481 gctcgcgccc gagccgccgc tcaaggacta cgtcgacctg ccggctatgg gcactctcgc
      541 ggcggaagag gtgcgaggcg tgctgcgtgc cctggtggat ctcgacgtcg atctggcgcg
      601 gcgcgttgcc gcgggcgaca acgagatcga tgcgctgtac caccagatct tcagcgaggt
      661 gctcgagctg atgcgccagg acccgaccaa cgtcgaccgc ggcacgcgca tcctgttcgc
      721 ggccaagaac ctcgagcgta tcggcgaccg cgtgaccaac atcgccgagg acgtggtctt
      781 cctggcgacc ggcgacatcg aggacctcaa cccatagaca tcggcaacga cgtggcgcga
      841 cccgacccgc agcgcgtgct cttcctgtgc gtccacaaca gcgctcgctc gcagatggcg
      901 gagggcctgt tgcgcgcccg cgcgggcgat cgattcgagg cgttcagcgc cggtaatgag
      961 gcgaccgaag ttcggccgtt ggccatcgcg gccatggctg agataggcat agacatcagc
     1021 ggccagcgca gcaagacgac cgcggacctg ggcgggcagc gcttcgacta cgtcatcacc
     1081 gtgtgcgacg acgcgaagga agcctgtcct tactacgcga acgcggcgca tcagatccac
     1141 tggggcttcg atgatccctc ggccgcgacg ggatccgatg atgagcggct cgcggtgttc
     1201 cgccgcgtgc gtgacgaaat tgctgcccgc atagacgaat tcgtaagcgc cgccggggcc
     1261 tgacgggtct aggctggcgc cgatggacgc aggcccagac ggcaccgatt tcgcgctcta
     1321 cctgatccgc cacgcgcatg ctggcaacgc cgccaactgg gaaggcgacg atgccgagcg
     1381 gcccttgagc gcgcgcggca aggaacaggc gcagaagctg ggccggctgc tcgccgcgcg
     1441 cggcgtggtg cccgacgcga tcgtgagcag ccccaaggtg cgcgcccgcc agacggccaa
     1501 cgtcgtcgcc gacgcgctgg gcgtcggtgt tgccagtgac gatcgcctcg cggagatgtt
     1561 cgatctggac gcgctcgcag cgatcatggc cggggtcggc ggcacgagcc tgatggtcgt
     1621 gggccacgat cccgacttct ccgagatgct cgcgacgctc gtcggcgcgg ccgagctgcc
     1681 catgcgcaag ggcgcgattg cgcgggtgga cgtggcgctg ccgctcggcc cgggctgcgg
     1741 catcctgcgc tggctcattc caccggagtt gctgcccgac caagcggacg actaaaagcg
     1801 tccgggcgga ccccggcctc ggccgggtgc cggccggtaa gcgcgtagca gcggcccgca
     1861 ggccagctgc gaagaaagca cattagcgac cggctacagt cccggccatg ctcgaggccg
     1921 ccctcgccgg tgccctagcc ggctacgcca tcgccatccc ggtcggcgcg atcgccgtgc
     1981 tgatcatcca tatcggcctg acccaaggtc tgcgccaggg actcgcggcg ggagcagggg
     2041 cagctacagc ggac
//
LOCUS       DATBRX010000059         7434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2811017, whole genome shotgun sequence.
ACCESSION   DATBRX010000059 DATBRX010000000
VERSION     DATBRX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811017"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VIF08_02080"
     CDS             complement(<1..237)
                     /locus_tag="VIF08_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134800.1"
                     /translation="MKRLIWLPIAGFLLIAGAAVAAAAPSLVDQARGQLGLGVQDSGP
                     VTVGDAELAHPGRDLLADVLADLVSNNTITQAQSD"
     gene            529..1716
                     /locus_tag="VIF08_02085"
     CDS             529..1716
                     /locus_tag="VIF08_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY8134801.1"
                     /translation="MAVLPEKATHQQTRTFNQQLVLRAIYDRHETSRAELARLTGLTR
                     TSVSALVGELLRDGLIEEVGTGPSTGGKAPILLRVRPDGRHTIGIDLTEPYFAGALVD
                     LRGHIIRSVRLPLDGRDGMAAVEVVMAVIEELANRNGRTPLLGIGIGAPGIVNSRDGT
                     IRWGVNLDWADVPLGAMVEQRYGLPAVVVNDSQAAAVAEFTFGTPPRPNNMVVVRVGR
                     GIGSGIILDGRLYQGDGAGAGEIGHTIYGATEPSADGERCRCGRVGCLEMAASMHAMV
                     TAAGRLEPSVTDAGSLVAALSARNENVRQVVVDAGHRLGQAIAALVSVLNVNLVLLIG
                     AATRLGDVWLNAVRERAASGALHQLTATTRIELGAARDEDDVLIGASALLMTRELGLS
                     LVR"
     gene            1713..2729
                     /locus_tag="VIF08_02090"
     CDS             1713..2729
                     /locus_tag="VIF08_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8134802.1"
                     /translation="MITHALSSTTPIATSRAMLTVGIDVGGTKTSCVVTDADDQLLMH
                     EVEPTHAEQLTEQLAALVQRAVDRIAIDARGTIAAVGVAVPGRVETHSGRIALAVNLG
                     GRDLDLGPQLERELGLPCFVEHDARAAAIWINAQDSAADDLAYLAIGTGVSTGIVLNG
                     RVLRGANGLAGEIGHVTAEPGGAPCACGLKGCLEVVAAGPHIARLAREAVASGRTTAL
                     PADPTAADVFRAAAAGDELATEIAASVAERLARAIRALVLTLGVNHIVIGGGVAAAGA
                     SLLGPVLAAIDAERAASPLVDAAFREASVELLPPQAEAGARGAAQIARERISARQQKG
                     VGER"
     gene            2801..4273
                     /locus_tag="VIF08_02095"
     CDS             2801..4273
                     /locus_tag="VIF08_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8134803.1"
                     /translation="MTTKRNWWLTAVGAVVLATACQTAAPSPSPSSQPGPTSTPAGPA
                     AVPAVPTGHAELDQALGADQPFKGKKVTIQTQWILGEGANFDASVADFEAATGIEIVQ
                     ESIASQHELVLRSRIEGHTPPDLAMLAQPSAVIEYGDRGLTTNVASLIDASQFSAGFG
                     TSLVTGAGGEIWGIPFKVDVKSTIWYPIKAFAAKGYAVPTTWAELQTLAAKIVSDGNG
                     SPWCVAIKDGQATGWIATDWLEDVMLRTAGVEKYNQWITHELPFDSPEVRAALAVIGE
                     QWFTQGYVYGGYQSITATDRLAPMDPMFPTDGNWEGFTPGCWMHRQATWFGPDFFPGV
                     RLNGQSGSEHPNVVGEDIGIMYFPSIDAAQGKPALGAGDTLMVLTPGDGSEVRPEVKA
                     VAEFLSTPYGLQRWIERGSAISANQTTPEAWYAGAYKLAVAADIVGNSTSFGFDASDL
                     MPPEANRAFWDGITAWIDAGGTPAATDTFVQSVDAAWPAQ"
     gene            4362..5534
                     /locus_tag="VIF08_02100"
     CDS             4362..5534
                     /locus_tag="VIF08_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY8134804.1"
                     /translation="MATTSRDTVEISPLRRFAPPLAAVLGLVIVAIGVGILADRNAAS
                     NLMASIYDALGNTQGAVALRNGAGDQLAAKLVLGVLALFVGVGGIWMLFVGVSAIVGL
                     LSPRWQGRILPWVFIVPAMALLTVFLIYPTVGTIVTSLTVQTNGDPLAHYKALFSPQY
                     LGLIQNNLTWLIVGTGGSVLIGLLIAGLVDRVKREALAKTFIFLPLAISFVGASVIWR
                     FMYEWQPGGQPQIGLLNAVVTSVGAGPIRWVQDQPLNTYALILIFIWLQTGFAMVVLS
                     AAIKGVSIEVIEAARLDGASERQIFTRVIVPIIKGSIITVATTIAIVVLKIFDIIYVM
                     TGGRDGTNVIANRMFQEFYEFFNDGRAAALGTVLFIAVLPVMFVNVRNLRKQGIGS"
     gene            5621..6430
                     /locus_tag="VIF08_02105"
     CDS             5621..6430
                     /locus_tag="VIF08_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8134805.1"
                     /translation="MVGICLLWSIPTLGLLVTSVRNPAEINTTGWWEALLNPFRENQW
                     TLSNYQSVIASEGMLSAFINSLVVTIPSTVIPITIAAFAAYAFAWINFPFRGLLFAIV
                     VGLLVIPLQMAFIPVLRLFGQFGLNGTFLGIWLAHTAFGLPLAIFLLYNFISQLPRDL
                     FESAAIDGASHFQVFIRLVLPLSIPALGAFAIFQFLWVWNDLLVALVFLGTGADQRVM
                     PSALNVLIGNRGGQWHLLTAGAFVTMLLPLAVFLILQRAFVRGILAGSVKS"
     gene            6427..>7434
                     /locus_tag="VIF08_02110"
     CDS             6427..>7434
                     /locus_tag="VIF08_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY8134806.1"
                     /translation="MTAERLMVAFAGTVLPEAVRIALGTDAYAGVSLFRHANVESPAQ
                     VRALTDALQAAARVRPLLIATDQEGGQLNALGDGPTRFAGAMALGAAADEDLAERVAA
                     ATAREMRALGVNVNYAPVCDLATNPLNPALGIRCFGDEPDAVGRLAAATVRGLQGEGV
                     AATVKHFPGLGDAPVDTHHDLAVVETTRAQLDARELVPFRAAIAAGARLAMAGHVAVP
                     ALTGAKDLPASLSAAALRGVLRDELGFAGLTITDALDMAGVNAGAADPLSAALAAGED
                     LLLGTPVVPLLGRDLQANDQAPVARLAALRAWLGRFGQPDLSVVGCAEHRALADELAR
                     RS"
BASE COUNT         1167 a         2488 c         2488 g         1291 t
ORIGIN      
        1 gtccgactgc gcttgggtga tcgtgttgtt gctgacaaga tcggccagta catcggccag
       61 caggtcgcgg cccggatggg ccagctcggc gtcgccgacg gtgacgggcc cgctgtcctg
      121 gacgcccagc ccgagctggc cgcgcgcctg gtcgacgagc gacggggcgg cggccgcgac
      181 ggcggcaccc gcgatcagca ggaagcctgc gatcgggagc cagatgagcc ttttcatggg
      241 cagagttacc tccgttgttg tcggacccca gtccgttccc cgatgtgttg atgctgccga
      301 tgatcacgac ggggactggg ccgcggctgt ctgcgtgctg gcgattggct gtgaatgcag
      361 cgcatcgcct gcgctgccaa agaggctttg tccattgact tgacatacat ggtccggtgt
      421 gcgacaaaat ggtgtttgtc aactgacttg gcaaagtctc gacgccgtgg gtggggagtc
      481 gaccggcgca ggaggcgaga ggagccaggc caggaccgga aggtgtccgt ggcggttctg
      541 cctgagaagg cgacgcatca acagacgcgc acgttcaacc agcagctggt gctgcgcgcc
      601 atctacgatc gacacgagac cagtcgggcc gagctcgccc ggctgacggg gctcacccga
      661 acgtcggtct cggcgctcgt cggtgagctg ttgcgcgacg gcctcatcga agaggtcggc
      721 accggaccgt cgaccggtgg caaggccccg atcctcctgc gggtgcggcc cgacggccgg
      781 cacacgatcg gcatcgacct gacggagccc tacttcgcgg gcgcgctcgt tgacctgcgc
      841 ggccacatca tccgttcggt gcgcctcccc ctcgacgggc gcgatggcat ggcggcggtg
      901 gaagtggtca tggcggtcat cgaggagctg gccaatcgca atggccgcac accactgctg
      961 ggcatcggca tcggcgcccc cggcatcgtc aactcgcgcg acgggaccat ccgctgggga
     1021 gtcaatctcg actgggccga cgtgcccctg ggcgcgatgg tcgaacagcg ctacggactg
     1081 cccgccgtgg tggtcaacga tagtcaggct gcggccgtgg cggagttcac tttcggcacg
     1141 ccgccgcgcc cgaacaacat ggtcgtcgtg cgcgtgggcc ggggcatcgg ctcgggcatc
     1201 atcctcgacg gccggctata ccagggcgac ggcgccggcg cgggcgagat cggccacacc
     1261 atctacggcg cgacggagcc gagcgcggac ggcgaacgct gccgctgcgg ccgagttggc
     1321 tgcctggaga tggcggcgag catgcatgcg atggtcaccg ccgcgggccg gctggagccg
     1381 tccgtcacgg atgccggctc gctggtcgcc gcactgagcg cccggaacga gaatgtgcgc
     1441 caggtcgtcg tcgacgccgg ccaccggctg ggccaggcaa tcgcggcgtt ggtcagcgtg
     1501 ctgaatgtca acctcgtgct gctcattggc gccgcgacgc ggctgggcga cgtgtggctc
     1561 aacgccgtgc gcgagcgcgc tgccagtgga gcgttgcacc agctcaccgc aactacgcgg
     1621 atagagctcg gcgccgcgcg tgacgaggac gacgtcctga ttggcgcatc ggccctgctc
     1681 atgacgcgcg agctgggcct gagtctcgtt cgatgattac ccacgccctg tcgtcaacga
     1741 cgcccatcgc caccagtcgc gcgatgctca ccgtgggcat cgacgttggc ggcaccaaga
     1801 cctcgtgtgt cgtgacggac gccgacgatc agctgctcat gcacgaggtg gagccgacac
     1861 acgccgagca gctgaccgaa cagcttgccg cgctcgtgca gcgcgcggtc gatcggatcg
     1921 cgatcgacgc ccgcggcacc atcgcggccg tcggcgtggc cgtgccgggc cgcgtcgaga
     1981 cgcacagcgg ccgaattgcg ctcgcggtga acctgggcgg gcgtgacctt gaccttggtc
     2041 cgcagctcga gcgcgagctc ggcctgccgt gcttcgtcga gcacgacgcg cgagccgccg
     2101 cgatatggat caacgcccag gacagcgccg ctgatgatct ggcgtacctg gccattggca
     2161 cgggcgtctc gacgggcatc gtcctgaatg gccgcgtgct gcggggtgcg aacggcctag
     2221 cgggcgagat cggccacgtc accgctgagc cgggcggcgc gccatgcgcg tgcggcctca
     2281 agggctgcct cgaagtcgtc gcggccggac cgcacatcgc gcgcctcgca cgcgaggcag
     2341 tggcgagcgg tcgcacgacc gcgctgccgg ccgatccgac cgcggccgac gtttttcggg
     2401 ccgcggccgc cggcgacgag ctcgccacgg agatcgcggc gagcgtcgcc gagcgcctcg
     2461 cgcgggcgat ccgcgcgctt gtcctgacgc tgggcgtcaa ccacatcgtg atcggcggcg
     2521 gggttgccgc ggccggcgca tcgctgctgg gccccgtgct cgctgcgatc gacgccgaac
     2581 gtgccgcttc cccgttggtc gatgcggcct tccgcgaggc gagcgtggag ctgctgccgc
     2641 cacaagccga agccggagcc cggggagcgg cacagattgc gcgcgagcgc atcagcgccc
     2701 ggcagcagaa gggggtgggc gaacgataaa aacaaggagg acagctatag agtcatcacg
     2761 tgaaacccaa ttgggccaaa caaactcgag gaggagtaat ttgactacga agcggaactg
     2821 gtggcttacg gcggtaggcg ccgtagtgct cgccacggcc tgccagacgg cggcgccgag
     2881 tccgtcaccg agcagtcagc ccggcccgac gagcaccccc gcgggacccg ccgcggttcc
     2941 cgcggtcccg accgggcacg ccgagctcga tcaggcactc ggcgctgatc agcccttcaa
     3001 gggcaagaag gtcacgatcc agacgcagtg gatcctcggc gagggcgcca actttgacgc
     3061 ctccgtcgcg gacttcgagg ctgcgaccgg cattgagatc gtgcaggaga gcatcgcctc
     3121 gcagcacgag ctcgtgctcc gcagccgaat cgaaggccat actccacccg atctggcaat
     3181 gctggcccag ccatctgccg tgatcgagta cggcgatcgc ggtctcacga ccaacgtcgc
     3241 ctcgctcatt gacgcctcgc agttctctgc tggcttcggt accagtctgg tcaccggcgc
     3301 tggcggcgaa atctggggta ttccgttcaa ggtcgacgtc aagtcgacca tctggtaccc
     3361 gatcaaggcc tttgcagcca agggttacgc cgtgccgacg acgtgggcag agctgcagac
     3421 cctcgcggca aagatcgtct ccgacggcaa cggctcaccg tggtgcgtcg cgatcaagga
     3481 cggtcaggcc acgggctgga tcgccaccga ctggctcgag gatgtaatgc ttcgaacggc
     3541 cggcgtcgaa aagtacaacc agtggatcac ccatgagttg ccgttcgatt cgcccgaagt
     3601 acgcgctgcc ctcgccgtga tcggcgagca gtggtttacc cagggctacg tttacggcgg
     3661 ctatcagtcg attaccgcaa cggaccggct cgcgccgatg gacccgatgt tcccgaccga
     3721 cgggaactgg gagggtttca cgcccggctg ctggatgcat cgccaggcaa cctggttcgg
     3781 gcccgacttc ttcccgggtg tccgcctcaa cggacagtcg ggatctgagc atccgaatgt
     3841 cgtcggcgaa gacatcggca tcatgtactt cccgtccatc gacgccgcac agggcaaacc
     3901 tgccctgggc gccggtgaca ccctgatggt cctcaccccc ggggatggct cggaagtccg
     3961 acccgaggtc aaggccgtcg cggagttcct gtcaacccca tacggtctgc agcggtggat
     4021 cgagcgcggt agcgcgatct cggcgaacca gaccactccc gaggcgtggt acgcgggcgc
     4081 ttacaagttg gctgttgcgg ccgacatcgt gggcaactcg acgagcttcg gcttcgacgc
     4141 gtctgacctc atgccaccgg aggccaaccg ggccttctgg gatgggatca ctgcgtggat
     4201 tgacgccggt ggcacacctg ctgccaccga cacgttcgtc cagagcgtgg acgcagcctg
     4261 gcctgctcaa tagccatcac ctagcctctt agtcaagggc gcgtccgcgg taacgcggcg
     4321 cgcccttgta acatcaccgc cgccgagcaa gggagacacg gatggcgacc acgtcccggg
     4381 atacggtcga gatcagcccc ttaagacgct tcgccccgcc attggcggcc gtgctcggat
     4441 tggtcatcgt tgccatcggg gttggcatcc tcgccgaccg taatgcggcc tccaacctga
     4501 tggcgtcgat atacgacgcg ctggggaata cccagggagc agtcgctctc aggaatggcg
     4561 ccggcgatca actggccgcc aagctcgtgc tcggcgtctt ggccctgttc gtgggtgtcg
     4621 ggggcatctg gatgctgttc gtcggcgtga gcgccatcgt cggcctcctc agccccaggt
     4681 ggcagggtcg aatcctcccc tgggtcttca tcgttccggc gatggccctg ctgaccgttt
     4741 ttctcatcta cccaacggtc gggacgatcg tcaccagcct taccgtgcag acgaacggcg
     4801 acccgttggc gcactacaag gcgcttttct caccgcagta cctcgggctc atacagaaca
     4861 acctcacctg gctgatagtg ggcactggcg gcagcgttct gatcgggctg ctgatcgcgg
     4921 gattggtgga tcgtgtcaag cgcgaggccc tggccaagac attcatcttc ctgccgctgg
     4981 cgatctcatt cgtcggcgcc tcggtgatct ggcgcttcat gtatgaatgg cagcctgggg
     5041 gtcagcccca gatagggttg ctcaacgctg tggtcacctc cgtgggcgca gggccgatcc
     5101 gctgggtaca ggatcagccg ctcaacacct atgcgctgat cctgatattc atctggctcc
     5161 agaccggctt cgcaatggtc gtgctttcag ccgcgatcaa gggggtctcg atcgaggtca
     5221 tcgaggctgc gcggctggac ggagcgagcg aacggcagat cttcacccgg gtgatcgtgc
     5281 ccatcatcaa aggctcgatc atcacggtgg caacgacgat cgcaatcgtc gtcctgaaga
     5341 tcttcgacat catctacgtg atgaccggcg gccgcgacgg caccaacgtg atagcgaatc
     5401 gaatgttcca ggagttctac gagttcttca acgacggacg cgcagcggcc ctgggaactg
     5461 tgctcttcat cgccgtgctg ccggtcatgt ttgtgaacgt gcgcaatctc cgcaagcaag
     5521 gcatcggctc atgacatcac cggcacgccc cgtagcgcga gtcaggtccg tcgcggcctg
     5581 gcgaccggcg ttcattcgaa gcgccccact gcggatcgcg gtggtgggga tctgtctgct
     5641 ctggtcgatc ccaactcttg gcctgctggt gacctcggtg cgcaacccgg ccgagatcaa
     5701 taccaccggg tggtgggagg cgctgctgaa ccccttccga gagaaccaat ggaccctgtc
     5761 gaactaccag tcagtcatcg cgagcgaggg catgctcagt gccttcatca acagcctggt
     5821 cgtgaccatt ccgtcaacgg tgatacccat cacgattgcg gcgttcgcgg cctacgcgtt
     5881 cgcgtggatc aacttccctt tccgcggtct cctgttcgcc attgtcgtag gactcctggt
     5941 catacccctg caaatggcgt tcatcccggt tctgcggctc ttcgggcagt tcgggctaaa
     6001 cgggacgttc ctgggcatct ggctggcgca cactgccttc ggccttcctc tcgcgatctt
     6061 cctgctctac aacttcatct cgcagctacc gcgcgacctt ttcgagtcgg cggcgattga
     6121 cggcgcgtcg cacttccagg tcttcattcg gcttgtcctg ccgctgtcga ttcccgccct
     6181 tggagcgttc gccatcttcc agttcctgtg ggtctggaat gacctcctgg tagcgcttgt
     6241 cttcctgggc acaggcgcgg atcagcgcgt catgccctct gccctcaatg tgctgatagg
     6301 caatcgcggt ggccaatggc acctcctgac cgccggcgcc ttcgtgacga tgttgctgcc
     6361 actggccgtg ttcctgattc tccagcgcgc attcgtacga ggaatcctgg ccggctccgt
     6421 caagtcatga ccgcggagcg gctcatggtc gctttcgccg gcaccgtgtt accggaggcg
     6481 gtccggatcg cgctgggcac cgacgcgtat gcaggcgtgt cgctcttccg tcacgccaac
     6541 gtggagtccc cggcacaggt gcgtgcgctg accgacgctc tccaggccgc cgcgcgtgtc
     6601 cgcccgctgt tgatcgccac tgaccaggag ggcgggcagc tcaacgcgct gggcgatggt
     6661 cccacgcggt tcgcgggcgc gatggccctg ggcgccgccg cggacgagga tctcgccgag
     6721 cgggtcgcgg cggccacggc gcgcgagatg cgcgcgctgg gcgtcaacgt caactacgcg
     6781 ccggtgtgcg acctggcgac gaacccgctc aacccggcgc tgggcatccg ctgcttcggc
     6841 gatgagcccg acgcggtcgg acggctcgcg gcggcaacgg tgcgcggcct ccagggtgag
     6901 ggcgtcgccg cgaccgtcaa gcacttcccg ggccttggtg acgcgccggt tgacacgcat
     6961 cacgatctcg ccgtcgtgga gacgactcgc gcgcaactcg acgcgcgcga gctcgtgccc
     7021 ttccgcgccg cgatcgctgc cggcgcgcgc ctggcaatgg ccgggcacgt cgcggtgccc
     7081 gcgctgaccg gtgccaagga cctgccggcg agcctttcag cggccgcgct gcggggcgtg
     7141 ctgcgcgacg agctcggctt cgccggcctg acgatcaccg acgcgctcga catggctggc
     7201 gtcaacgccg gcgcggccga tcccctatcg gccgccctgg ccgcgggcga ggacctgttg
     7261 ctgggtacgc cggtcgtgcc cctcctcggt cgcgatcttc aggcgaacga ccaggcaccg
     7321 gttgcccgcc tggcagccct gcgggcctgg ctcggtcggt tcggacagcc cgatctgtcc
     7381 gtggtcggct gcgccgaaca tcgcgctctc gcagacgagc tcgcgcggcg ctcg
//
LOCUS       DATBRX010000060         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1470543, whole genome shotgun sequence.
ACCESSION   DATBRX010000060 DATBRX010000000
VERSION     DATBRX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470543"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VIF08_02115"
     CDS             complement(<1..536)
                     /locus_tag="VIF08_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134807.1"
                     /translation="MRLVEIRSLDGPNIYRLEPAVRVEVAVGRRRTWYGQRLPARHAL
                     VRLGAPVRPSDAPSGVVALAHAVRRLHRVAVARRVPVTIHRTSEPGSWVVAFPWQQRD
                     QAELIARAAFRLAETPSERLFARAVEQARGADGEPPEWITDEARASAERRGRGRVPLI
                     SISGTNGKSTTTRMISHIA"
     gene            576..1784
                     /locus_tag="VIF08_02120"
     CDS             576..1784
                     /locus_tag="VIF08_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY8134808.1"
                     /translation="MVTDSQLDELRSAVAAALPEYLADLERLVNIDCGSYTKHGVDEV
                     GRWVADQLRELGAEMRVLPHATYGDTVVGVLEGRGRGRALLIGHLDTVFEPGTAAERP
                     FVIDDGRAYGPGVDDMKGGLLSGLYALRALRSTRGGGVPADWLPFERLTFVANPDEEI
                     GSPSSTPHIKEAARDATVAFVLESARENGDIVSARKGIADFRLTISGRAAHAGVEPEK
                     GRSATLEAAHKTVALHALNGRWEGVTVNVGAIHGGTRPNIVAEKCELSVDVRATSVNA
                     LDEVEAAIRDICETFTVSDTTCIIERIAHWPPMEKTPATGALAELAISLAARIGFELH
                     DAATGGASDANTTSGMGVPSLDGLGPVGGADHAPGEYLEVDSIVPRTTLFAALLLAVA
                     EPEADFSASS"
     gene            complement(1781..2152)
                     /locus_tag="VIF08_02125"
     CDS             complement(1781..2152)
                     /locus_tag="VIF08_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134809.1"
                     /translation="MSRRGVAVVVVLAIAVPLVVLLRSFTYVPFLNDGGPDVHVAAEL
                     PDRIRVCGRTYGHHGIGAHHTLAQVKEMTDHPAFVNPWIFAACPRYGYFEEDGERIVT
                     TVMFVRVGEDAYDTYALSGGP"
     gene            complement(2168..3895)
                     /locus_tag="VIF08_02130"
     CDS             complement(2168..3895)
                     /locus_tag="VIF08_02130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEY8134810.1"
                     /translation="MNKRAPLLRRALCLAVIGLLAACSGQPAAQQASTANAPVVAGAS
                     EPKAVEIGPTAAGEQVQFSVSLRLPGTAELDTYLHDLVTPGTSNYRQFLAPEEFGARF
                     GLSDTRIAPILAWLANAGLAGDLMPQRTSIAVSGSAGQVSDLLGVKLVDWKNAAGKRY
                     HVPVGDVTVPANLGGDVAAVIGLNTEPIIQPSAHPTPIKAGAGEGLVPSTVATAYEIE
                     ALHDAGLNGEGMTIAIVSFATFTPSDVELFDETFGYAGGTPVELVRLPGAITELDEGS
                     GEVALDIQVIRGIAPRAQIINYEGPNTSDGFAGIMARIVADGRAKIVSISWGLCENWS
                     APSSMNAEQREFAAAFAAGISIFSSSGDDAAYDCRRVDISDNPFERDLSPGVDWPTSS
                     PSVISVGGTFLTLREDGTYVEEAGWEEPLGGGGGGGGLSGIQERPEWQVGLGVDNEAS
                     NGKRQVPDVAGPADPTSGFVVTYTDSELGLVSGQVGGTSAAAPFWAASMLLTQQLAAR
                     EGVAALGPLGPVLYQISAEQPADAVFHDIVRGGNLLYSAGPGWDYSTGLGSPRVAPLA
                     RAIVDVLLR"
     gene            complement(3892..>4420)
                     /locus_tag="VIF08_02135"
     CDS             complement(3892..>4420)
                     /locus_tag="VIF08_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134811.1"
                     /translation="PTPVTNSPILLSGELWQSSALNYSFEYDPNRWSLEDSSEDTAVF
                     YSVRYDAQLVIHAVPAQTSPAELIAQQLEIIDRIMVGRTKDTDDYDALLGPSIGYVDG
                     EGDVWSGVLLSQDGTPIAPGGVTVMAATNGRLTVSVLVLVGSPDAQVGSGTQQHRVRG
                     QADEFLKSFDWDTGS"
BASE COUNT          709 a         1498 c         1513 g          700 t
ORIGIN      
        1 gcgatgtggc tgatcatgcg cgtggtcgtg ctcttgccgt tggtgccgct gatgctgatg
       61 agcggcaccc gaccgcgacc gcgccgctca gcgctggccc gcgcctcgtc ggtgatccat
      121 tcgggcggct ccccatccgc accacgggcc tgttcgacgg cccgcgcaaa gagccgctcc
      181 gatggcgtct ctgccagtcg gaacgcggcg cgcgcgatga gctccgcctg gtcgcgctgc
      241 tgccacggga aggcgacgac ccagctgccc ggttcgctgg tgcggtggat cgtcaccggc
      301 acgcgtcgcg caacggcgac gcggtgcagc cgccgcacgg cgtgcgcgag cgcgacgaca
      361 ccggatggag catcgctggg acggactggc gctcccagcc gaaccagcgc gtggcgtgcg
      421 ggaaggcgct ggccgtacca cgtccgcctt cggccgactg cgacctcgac gcgcacggcc
      481 ggctcgaggc gatagatgtt gggaccgtcg agtgaacgga tttcgactaa gcgcacggtg
      541 cgccggagta tatgaacctc cgataccctg ccgccatggt caccgattca cagctcgacg
      601 agctgcgctc cgccgtcgcg gctgcgctgc ccgagtacct ggccgacctt gagcggctcg
      661 tcaacatcga ctgcggcagc tacaccaagc acggcgtcga cgaggtcggc cggtgggtcg
      721 ccgaccagct gcgcgagctg ggtgcggaga tgagggtcct gccgcacgcg acttacggcg
      781 atacggtggt cggcgtcctg gaagggcggg gcaggggcag ggcgctgttg atcggccacc
      841 tcgacacggt gttcgagccg ggcaccgccg cggagcggcc cttcgtgatc gatgacgggc
      901 gcgcctacgg gccgggcgtg gacgatatga agggcggact gctgtccggc ctgtacgccc
      961 tgcgcgctct gcgctcgacg cgcggcggcg gcgtgcccgc tgactggttg ccgttcgagc
     1021 gcctgacgtt cgttgccaac ccggacgagg agatcggctc gccgtcgagc acgccgcaca
     1081 tcaaggaagc ggcgcgcgat gcgaccgtcg cgttcgtgct cgagtcggcg cgcgagaacg
     1141 gcgacatcgt gagcgcgcgc aagggcatcg ccgacttccg cctgactatc tccggtcgcg
     1201 cggcgcatgc cggcgtcgaa ccggagaagg gccgcagcgc gaccttggaa gcggcgcaca
     1261 agacggtcgc gttgcatgcg ctcaatggcc gctgggaggg tgtcaccgtc aacgtcgggg
     1321 cgatccacgg cggtacgcgg ccgaacatcg tggccgaaaa gtgcgagttg agcgtggacg
     1381 tgcgcgccac gtccgtgaac gccctcgatg aggtcgaggc cgccatccgc gatatctgtg
     1441 aaacgttcac ggtgtcagac acgacatgca tcatcgagcg gatcgcccac tggccgccga
     1501 tggagaagac gcccgcgacc ggggcactcg ccgagctggc gatcagcctg gcggccagga
     1561 ttggcttcga gctgcacgac gccgcgaccg gcggcgcgtc agacgcgaac actacgtcgg
     1621 gcatgggtgt gccgtcactc gatggcctgg gtccagtggg cggtgccgat cacgcgccgg
     1681 gcgagtacct ggaagtcgac tccatcgtgc cccggacgac cctattcgcc gccctgcttc
     1741 tggccgtggc cgagcctgag gccgacttca gcgcctcgtc ctaaggaccg ccggacagcg
     1801 cgtaagtgtc gtaggcgtcc tcgcccacac ggacgaacat cacagtcgtg acaatcctct
     1861 ccccgtcctc ttcgaaatac ccatagcgag gacaggcggc gaatatccag gggttgacga
     1921 aggccgggtg gtcggtcatt tccttcacct gtgccagcgt gtggtgagca ccgatcccgt
     1981 gatggccgta ggtccgtcca caaactcgaa tgcgatcagg cagctcagcg gctacgtgca
     2041 catcgggtcc gccgtcattg aggaacggca cgtacgtgaa gcttcggagc aggacaacaa
     2101 ggggaacggc gatggccagg accacgacga ccgcaacgcc acgccgagac atcgcgcggc
     2161 gctcccgcta tcgcaacagt acgtcgacga tggcgcgggc cagcggggcg acgcgcggcg
     2221 agcccaagcc ggtcgagtaa tcccagccgg gtccggccga gtagagcagg ttgccgccgc
     2281 gaacgatgtc gtggaatacg gcgtcggccg gttgctcggc tgagatctgg tagagcaccg
     2341 ggccgagcgg tccaagggcc gcgacgccct cgcgcgcggc gagctgctgg gtaagcagca
     2401 tcgacgccgc ccagaatggc gcggccgcgc tcgtgccacc cacctggccg ctgaccaggc
     2461 ccagctcaga atcggtgtaa gtgaccacga agccgctcgt cgggtcggcc gggccggcga
     2521 catcgggcac ctggcgcttg ccgttgctgg cctcgttgtc gacccccagg ccgacctgcc
     2581 attccggtcg ctcctggata cccgacaggc cgccaccgcc gccgccaccg ccgagcggct
     2641 cttcccagcc ggcttcttcg acgtaagtgc catcctcgcg cagagtcagg aaggtgccgc
     2701 ccacgctgat cacgctcggg ctcgatgtcg gccagtccac gcccggcgac aggtcgcgct
     2761 cgaacgggtt atccgaaata tcgacacgcc ggcagtcgta ggccgcgtcg tcaccggatg
     2821 aggagaagat gctgatgcct gccgcgaacg cggccgcgaa ctcgcgttgc tcggcgttca
     2881 tgctcgacgg tgccgaccag ttctcgcaca ggccccagct gatgctcacg atcttggccc
     2941 tgccatcggc gacgatgcgc gccatgatcc cggcgaagcc atcggaggta ttggggccct
     3001 cgtagttgat gatctgcgcc ctgggcgcga tgccccggat cacctggata tcgagcgcca
     3061 cttcgcctga cccctcatcg agctcggtaa tcgcgccggg caggcggacc agctcaacag
     3121 gtgttccgcc ggcgtagccg aaagtctcgt cgaagagctc gacgtcgctg ggcgtgaacg
     3181 tggcgaacga gacgattgcg atcgtcatgc cctcgccgtt caggccggcg tcatggagcg
     3241 cctcgatctc gtacgcggtc gcgacggtgc tgggcaccag cccctcgccg gcacctgcct
     3301 tgatcggggt gggatgtgcc gacggctgga tgatcggctc tgtgttgagc ccgatcacgg
     3361 ccgcaacgtc gccgccaagg tttgccggca cggtgacgtc gcccaccggc acgtggtagc
     3421 gcttcccggc ggcgttcttc cagtcgacca gcttgacgcc gagcaggtcg ctcacctgac
     3481 cggcgctgcc gctgaccgcg atggaagtgc gctgcggcat caggtcgccg gcgagaccgg
     3541 cgttggccag ccaggccaag atgggtgcga tccgcgtatc ggacagccca aatcgcgcac
     3601 cgaactcctc gggcgccagg aattgccggt agttgctcgt gccgggcgtc accaggtcgt
     3661 gcaggtatgt atcgagctcg gccgtcccgg gcagccgcag gctgacgctg aactgcacct
     3721 gctcgcctgc ggcagtaggc ccaatctcga ccgccttggg ttccgaagcg cctgcaacca
     3781 ccggcgcatt ggctgtgctc gcctgctgtg ctgcgggctg cccgctgcac gccgccagca
     3841 gtccaatcac ggccaaacac agggctcgcc ggagcagtgg cgctcgcttg ttcatgaccc
     3901 tgtgtcccaa tcgaaggact taaggaactc gtcggcctgg ccgcgcacac ggtgctgctg
     3961 tgtgcccgat ccgacctggg catcaggcga gccaaccagg accagcaccg acaccgtgag
     4021 ccggccgttg gtcgcggcca tgacggtcac gccgcccggc gcaatgggcg tgccatcctg
     4081 gctcagcagc acgcccgacc agacatcgcc ctcgccgtcg acatagccga tgctggggcc
     4141 gagcagtgcg tcgtaatcgt cggtgtcctt tgtccgaccg accatgatcc tgtcgatgat
     4201 ctctagctgt tgcgcgatca gctcagccgg cgatgtctgc gccggcaccg cgtggatgac
     4261 gagctgcgcg tcgtaccgga ccgagtagaa gaccgcggtg tcttcgcttg agtcctcgag
     4321 cgaccagcgg ttggggtcgt actcgaacga gtagttgagc gcgctgctct gccacagctc
     4381 gcccgacagg aggatcggtg agttggtgac cggcgtcggc
//
LOCUS       DATBRX010000061         9168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3817374, whole genome shotgun sequence.
ACCESSION   DATBRX010000061 DATBRX010000000
VERSION     DATBRX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3817374"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VIF08_02140"
     CDS             complement(<1..188)
                     /locus_tag="VIF08_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134812.1"
                     /translation="MTEQYIGWALVVGLVIGGALVWFAIGRLPRSGEEIPQAERHVEA
                     EWISRTIVKRGGVAPPDLV"
     gene            216..1142
                     /gene="lgt"
                     /locus_tag="VIF08_02145"
     CDS             216..1142
                     /gene="lgt"
                     /locus_tag="VIF08_02145"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8134813.1"
                     /translation="MVGLINITPGSQVIPGFFIGWYGLLYVVGLAVLLLLSQREAERR
                     GYDPSHVFNAFLVVAAFALIGARLYHVIDQWGPIYSQDPLKAILPPYAGLGLYGGIAG
                     AIVGILLYVRWKRLPFRIALDVVIPGTFFAQGIARWGNFFNQELYGPPTDLPWGITID
                     CAHRVAQYLCPPGSLPTDVPLEGFHPLFFYESSLNIIGGLIALYVSRRYLKRLQPGDL
                     AAFWLIWYGTVRSFLEIFRAGWNWTLGGIATAQIIGIAIVAIGVAWLLYNHRRGTTPY
                     PYLPAYVAPPDEEHTLERDDRLYEEEEEEENP"
     gene            complement(1107..2567)
                     /locus_tag="VIF08_02150"
     CDS             complement(1107..2567)
                     /locus_tag="VIF08_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134814.1"
                     /translation="MASLATSQIPHADDTVDGRSVVDGGETVLAVAISLSSVGPNAYD
                     YDGSQSHLQFQVRGEPGTGPIGLSGAVEVQIVRLPGEPQPAAWSTTAATVPLPLDGCA
                     PSCILDFAVHIRAAQPDSVPLVVPWRIYAIAIGDVSRLVIDVDAVPQGPLLTDLAIWS
                     TSLGLFVALVVAMAVWAVGRGRTWSASALLEAIAALVLIGGGALVAQGLLVNLKSGMA
                     GLFEIGSGTAVVIGLTLNRPLLSRASAWLVPLALVLPPLALAQFVMGGEFRVADVIAA
                     FVATGVALVIAMILLVPRVRTRIAPLKLTQPRTLMLIALALAGAGVAAVWATLISAGG
                     TRVGVGVWAPLEFSYMALLIGLWRWLAGDSVTPFIAGFAAAVAAVVSAFMTVMSFFAN
                     LGVPGPASPLLIEAVGAVALSGVLFAIFTTLPPRRKDPAAHRGRAFQTGWAIVDATAS
                     GSAQPGSNDRLLEVVAVGIVGVAFVLGILLFFLFFI"
     gene            complement(2618..3046)
                     /locus_tag="VIF08_02155"
     CDS             complement(2618..3046)
                     /locus_tag="VIF08_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134815.1"
                     /translation="MVAGDGLLHPVAMAAIALLILNDHVLKSAYPGPITGKISDFMGL
                     LFFPLAVQAAVEIGARIVGRDATANQRVLLVAIAATGIVFTAVKLIPEINVVAELAIG
                     WLQVADTRPRIALDATDLIALPALLMAWHIGAGRVRADLA"
     gene            complement(3166..4272)
                     /locus_tag="VIF08_02160"
     CDS             complement(3166..4272)
                     /locus_tag="VIF08_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8134816.1"
                     /translation="MSGVALIVDDSAVNRMLLVRHLASLGIDAREAVDGKSALESLRG
                     QSADLAVVLLDVVMPEMDGYEVLAAIKSDEALRHLPVIMISGIDELDSVARCIELGAV
                     DFLPKPFNPVILGARVRASLADKRLRDLEAESAAAQARLLATIERQKEELSRFLSPQV
                     AALVSSPEGEQLLAGHRREATAVFCDLRGFTSFSDSAEPEEVLGLLREYHKQMGELIV
                     DHGGTLEHFAGDGMMVFFNDPVPQPDHVARAVRMAVAMRDRFATLAARWAKLGYELGF
                     GVGIATGFATLGRIGFEGRYDYGMVGAAVIVAARLSAKAEANQILLNPRAHAAVEELV
                     EVEDVGALELKGFARPIPTVNVRGLVASAESVPA"
     gene            complement(4269..4631)
                     /locus_tag="VIF08_02165"
     CDS             complement(4269..4631)
                     /locus_tag="VIF08_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8134817.1"
                     /translation="MSDELPILDVSGLAELRASVGDDENFVRDLVGTFLAEEPNNLEL
                     VAAAAARGDAEAVIRPAHTLKSSAAAIGAARLSSICRQLEFAGREGRSGDLAALTEEA
                     RAAWAATVEALRAQGLAT"
     gene            complement(4628..9163)
                     /locus_tag="VIF08_02170"
     CDS             complement(4628..9163)
                     /locus_tag="VIF08_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8134818.1"
                     /translation="MSFTYGVERGRRLENGTFPLIGPRRRVAETREPLVINEDAEARV
                     RELGQEGALEGEMARSALWVPLASGDEVQGIISLQNLDRENAFGEADVRLLTTLASSL
                     SVALENARLFDETQRLLTETNERAAELAIINSVQESLAAKLDMQSMYELVGDKIREIF
                     DAQVLDIGIYDFENRLTRYPYTIEKGQKLPDDPAPFGPNALWFIEHQKTNPAPVNIGN
                     IEKWVEETGLTFSIIGEPSKSIVFAPLISSGKLFGRISLQNLDRYDAFSESDVRLLAT
                     LAGSLSVALENARLFDETQRLLTETNERAAELAIINSVQQGLAEKLDMQAMYELVGEK
                     ITEIFDAQAMVISTVDVERGEMTWHFAVERGVRQDVVVLPLNSFAQYLIDHPEPLLVN
                     SGIREWEAEQGIESVVVGEPPKSMLFAALMRGGKVIGDISIQNLDHEFAFGEADLRLL
                     TTLAGSLSVALENARLFDETQRLLTETNERAAELAIINSVQEGLAANLDMQAMYELVG
                     DKIQEIFDAQVVDIGLFDFDRQMVSFPYTKERGVRFPDQPIAITQTARQLIAEKKPVV
                     IDDAVAWQEARGVPATIQGEAPQSMVFAPLMSGDRVFGRISLQNLDRKSAFSEADVRL
                     LSTLAASLSVALDNARLVAETRQRAAELSIVNELGQATASQLDLEKLIDLAGPQMAAT
                     FQADIAYVALVDPNTNMIEFPFYVENGVNEPQEPLPLGQGMTSRIIQARQPVLLNKAS
                     DHEDMASHGVGTIARSYLGVPILAGDKAIGAVSVQSVTQEGRFGESDVRLLTTLAANI
                     GSAIQNALLYGESQRRATEMSTLADVAREISATLDLGTLLPRIANQASDLLDGTSAAV
                     FLRDPDGKRFRAAAAVGSIAEQIKQTTLELGVGIIGTLAAEARAEVINDVASDKRAIQ
                     IAGTPQMATERLIVAPLIGRAGVNGMMAVWRTGNYRAFTPHDLDFLVGLSQNTAIAID
                     NAQLFAELREAREAADAANQAKSAFLAAMSHEIRTPMNAIIGMSGLLSDTELNVEQRD
                     YADTIRSSGDALLTIINDILDFSKIEAGKVDLDAEPFSPADCIEDALDVIAPAAAAKG
                     VELAYEVDGDLPPAVVGDLGRLRQIMLNLLSNAIKFTEKGEVLVSVSSQVDRSEVELR
                     VAVKDTGIGIAKDQMTRLFQSFSQADSSIARKYGGTGLGLAISRRLAEAMNGALVAES
                     SGRAGEGSTFRLTVRLPSAPASALTAVPARRTVELAGKRALIVDDNATNRRILKAQLA
                     RWDIGVRDTASPEEALKWIRQGERFDICLIDLFMPVMDGVALGDAIRAALPENTPKMI
                     LVSSAAMREHGANMDALLPKPIKPSALHDTLVTVFSSAEGRIQIQRAPEAAADRELAT
                     RHPLHILLAEDNAVNQKLALRLLANMGYVAEVANDGAQAIAALEAIDYDTILMDVQMP
                     ELDGLEATRRIRRQWPDRKVHIVAMTANAMAGDREACLAAGMNDYVSKPIKPAELAAA
                     LMRAPSNGTTKGEEGAS"
BASE COUNT         1539 a         3051 c         2991 g         1587 t
ORIGIN      
        1 accaggtccg gcggggcgac gccgccgcgc ttgacgatcg tgcgactgat ccactcggct
       61 tcgacatggc gctcggcctg ggggatctct tcgccgctac gtggtagccg gccgatggca
      121 aaccagacga gcgctccccc gatgaccagg cccaccacga gggcccagcc gatgtactgc
      181 tcagtcatcg agccgagtat aggtatgctg cgcggatggt cggtctgatc aatatcaccc
      241 ccggctcgca ggtgatcccg gggtttttca tcggctggta cggcctgctc tatgtcgtgg
      301 gcctggcggt gctgctgctg ctgtcgcagc gcgaggcaga gcgccgcggc tacgacccca
      361 gccatgtctt caacgcattc ctggtggtcg ccgcctttgc gctgatcggc gcgcggctct
      421 atcacgtgat cgaccagtgg ggccccatct acagccagga tccgctgaag gcgatcctgc
      481 cgccctatgc cgggctcggc ctgtacggcg gtatcgcggg cgccatcgtc ggcatcctgc
      541 tgtacgtgcg ctggaagcgc ctgccgttcc ggatcgcgct cgacgtggtc atcccaggca
      601 cgttcttcgc ccagggtatc gcgcgttggg gcaatttctt caaccaggag ctgtacgggc
      661 cgccgaccga tctgccgtgg ggcatcacaa tcgactgtgc ccaccgcgtt gcgcagtacc
      721 tctgcccgcc cgggtcactg cccaccgacg tgccgctcga aggctttcac ccgctcttct
      781 tctacgagtc ctcgctcaac atcatcggcg gactgatcgc gctgtacgtg tcgcggcgtt
      841 acctgaagcg cctccagccg ggcgatctcg cggcgttctg gctgatctgg tacgggacgg
      901 tgcgatcctt cctcgagatc ttccgcgccg gctggaactg gacgctgggc ggcatcgcga
      961 cagcccagat catcggcatc gcgattgtgg ctatcggcgt cgcctggctg ctctacaacc
     1021 accgtcgcgg cactacgcca tacccgtacc tgcccgcgta cgtcgcgccg ccagacgagg
     1081 aacacacgct cgagcgggac gatcgcctat atgaagaaga ggaagaagag gagaatccct
     1141 aagacgaagg cgaccccgac gatcccgaca gcgacgacct caagtagacg gtcgtttgag
     1201 ccgggctggg cagatccgga tgcggtggcg tcaacgatgg cccatccagt ttggaacgct
     1261 cgacctctat gagcggcggg gtccttcctg cgtggaggca gggtcgtgaa gatcgcaaac
     1321 aacacgccag agagcgcgac ggcgccgacg gcttcgatca gcaggggaga ggccggtcct
     1381 ggcacaccca ggttggcgaa aaaactcatc acggtcatga acgccgagac gacggcggcc
     1441 accgccgctg cgaagccagc gatgaatggc gtcaccgagt cacctgcgag ccatcgccat
     1501 agaccaatca gcagcgccat gtaactgaac tcgagtggag cccagactcc gacgcccacg
     1561 cgcgttcccc ctgcggagat cagcgtcgcc caaacggcgg cgactccggc gcctgcgagc
     1621 gcgagcgcga tcaacatcag cgtcctaggc tgcgtcaact tcagtggcgc gatgcgggta
     1681 cggacgcgcg gcaccagcaa aatcatcgcg ataaccaggg cgacgccggt cgcgacgaat
     1741 gccgcgatca catccgcgac ccgaaactcg cctcccatga caaattgagc gagggcgagc
     1801 ggcggcagga cgagggccag aggaaccagc cacgctgatg cgcgcgacaa cagcggtcgg
     1861 ttgagggtca ggccgatgac cacagcggtg ccagatccga tctcgaacag cccggccatc
     1921 cccgacttga ggttgaccaa tagaccttgc gccacaagcg ctccgccgcc gatcaatacg
     1981 agggcagcaa tggcttccag gagggccgag gctgaccacg tacgccctcg gccgacggcc
     2041 cagacagcca tggctaccac cagcgccaca aagagcccga gcgacgtcga ccaaattgcc
     2101 aggtcggtca gcaagggtcc ctggggaact gcgtcaacat cgatcacgag tcgacttacg
     2161 tccccgatag cgatcgcata gatccgccat ggaacgacga gcgggacgct gtcgggctgc
     2221 gcggctcgga tgtgaacggc aaagtccaaa atgcaactgg gcgcacagcc gtccagcgga
     2281 agcggcactg ttgccgcggt cgtgctccaa gccgcaggtt gtggctcgcc gggcaggcgc
     2341 acgatctgga cctctacggc gccagacagc ccgatcggtc ccgtgcccgg ctcacctcgg
     2401 acctggaact gaaggtggct ctgggaaccg tcgtagtcat acgcgttggg tcccacgctg
     2461 gaaagcgata tggctactgc caaaacggtt tcgccgccat ctacgactga gcgcccatcg
     2521 accgtgtcgt ccgcatgggg aatctgggat gtggccagcg acgccacccc ggcgacaact
     2581 agaagcagca gggccgctac ctgacgagga gttgaagtca cgccaagtcc gcccggactc
     2641 gacccgcgcc gatgtgccac gccatcaaga gcgctggcag tgcaatcagg tccgtcgcat
     2701 ccagcgcgat gcgcgggcga gtgtcggcga cttggagcca accaatggcg agctcggcca
     2761 ccacattgat ctctgggatc agcttgacgg ccgtgaacac gatgccggtc gcggcaatag
     2821 cgacgaggag gacacgttgg ttggccgtgg catcacgacc gacgatccgg gcgccgattt
     2881 cgacagcggc ctgtacggcc agcgggaaga acagcaaccc catgaagtcg gagatcttgc
     2941 ctgtgatggg tcccgggtat gcgctcttca gaacgtggtc gttcaggatc agtagggcaa
     3001 tcgccgccat ggccaccggg tgtagcaggc cgtcacccgc gaccatcgga gatctggccc
     3061 tgacggctgt cgccacaggc acagactcag cccgacgcgc gcgcgcatca aggtccattc
     3121 ggcattctta tcgcaattcg gtgcccacga ccgtggggcc cgggttcagg ccggaaccga
     3181 ctccgcgctg gcgaccagcc cgcgcacatt gaccgtgggt atcggtcggg cgaagccctt
     3241 cagctccagc gcgccgacat cctcgacctc gaccagctcc tcgacggcgg cgtgggctcg
     3301 tgggttgagc aagatctgat tggcctcggc cttggctgag aggcgcgccg cgacgatcac
     3361 cgccgcaccc accatgccgt agtcgtaacg gccctcgaag ccgatccggc ccagcgtcgc
     3421 gaagcccgtt gcgatgccca cgccaaaacc aagctcgtag ccgagcttcg cccaccgcgc
     3481 agcgagtgtc gcgaagcgat cgcgcatcgc gaccgccatc cgtacggcgc gtgcaacgtg
     3541 gtcgggctgc ggcaccgggt cgttgaagaa gaccatcatg ccgtcgccgg cgaagtgctc
     3601 gagcgtgccg ccgtggtcga cgatcagctc gcccatctgc ttgtggtatt cgcgcagcag
     3661 accgagcacc tcctccggct cggccgaatc gctgaagctc gtgaagccgc gcaggtcgca
     3721 gaagacggcg gtcgcctcgc gccggtgacc agcgagcagc tgctcaccct ccgggctcga
     3781 gaccagcgcc gcgacctgcg gcgacagaaa gcggctcagc tcttccttct ggcgctcgat
     3841 cgtcgcgagc aggcgcgcct gcgcggcggc gctctccgct tccaggtcgc gcaaccgctt
     3901 gtcggcgagc gatgcccgca cgcgcgcgcc caggatgact gggttgaacg gcttgggcag
     3961 gaaatcgacc gcgcccagct cgatgcagcg cgcgaccgag tcgagctcat cgatgccact
     4021 gatcatgatc accggcaggt ggcgcagcgc ctcgtcactc ttgatcgcgg cgaggacctc
     4081 gtagccatcc atctcgggca tcaccacgtc gagcagcaca accgccaggt ccgcggactg
     4141 gccgcgcagt gattcgaggg ccgacttgcc gtcgaccgct tcgcgcgcgt cgatgccgag
     4201 cgacgccagg tgacgcacca acagcattcg gttgacggcg ctgtcatcga cgatcagcgc
     4261 gacgccactc aagtcgcgag tccctgtgcg cgcagcgcct ccaccgtggc cgcccatgcc
     4321 gcgcgggcct cttcggtgag cgctgccaga tcccctgagc ggccctcgcg gcccgcgaac
     4381 tcgagctggc ggcagataga ggacaggcgc gcagcgccga tggcggcggc gctcgacttc
     4441 agcgtgtgcg ccggccggat gaccgcttcg gcgtcgccgc gcgccgcggc cgccgcgacg
     4501 agctcgaggt tgttcggctc ttcggcgaga aaagtgccga ccaggtcgcg cacgaaattc
     4561 tcgtcgtcgc cgaccgatgc gcgcagctcg gcgagtccgg acacatcgag gatcggcagc
     4621 tcgtcgctca tgacgctccc tcttccccct tcgtggtgcc attcgatggg gcgcgcatca
     4681 gggccgcggc aagctcggcc ggcttgatgg gcttgctgac atagtcgttc atgcctgccg
     4741 cgaggcacgc ctcgcggtcg cctgccatgg cgttggccgt catggcgacg atgtgcacct
     4801 tgcgatcggg ccactggcga cggatgcgcc gggtcgcctc gaggccgtcg agctcgggca
     4861 tctggacgtc catcaggatc gtgtcgtagt cgatcgcctc gagcgcggcg attgcctggg
     4921 cgccgtcgtt ggcgacttca gccacgtagc ccatgttcgc caggaggcgc agcgccagct
     4981 tctggttaac ggcgttgtcc tcagcgagca ggatgtgtag cgggtggcgc gtcgccagct
     5041 cgcggtcggc cgcggcttct ggagcgcgct gtatctggat gcggccttca gcgctcgaga
     5101 agacggtcac gagcgtgtca tgcagcgccg acggcttgat cggcttgggc aggagcgcgt
     5161 ccatgttggc gccgtgctcg cgcatcgcgg ccgacgacac caggatcatc ttgggcgtgt
     5221 tctcgggcag cgcggcacgg atggcatcac ccagcgcgac tccatccatc accggcatga
     5281 acaggtcgat cagacagata tcgaagcgct cgccctggcg gatccacttg agcgcctctt
     5341 ccggcgatgc ggtgtcgcgc acgccgatgt cccagcgcgc gagctgtgct ttcaggatgc
     5401 gtcggttggt cgcattgtcg tcgacgatca gcgccctctt gccggcgagc tcaacggtcc
     5461 gccgcgccgg gaccgcggtc agggccgagg ccggggcgct gggcagccgg acggtcagtc
     5521 ggaaggttga cccttcaccg gccctgcccg agctctcggc gacgagcgcg ccattcatcg
     5581 cttcggcgag ccggcggctg atggccaggc ccagaccggt gccgccgtac ttgcgcgcaa
     5641 tggatgagtc ggcctggctg aaggattgga agagccgggt catctggtcc ttggcaatgc
     5701 cgatgccggt gtccttgacc gcgacccgca gctcgacctc actgcggtcg acctggctgc
     5761 tgaccgagac gagcacctcg cccttctcgg tgaacttgat cgcgttcgag agcaggttga
     5821 gcatgatctg gcgcaagcgg cccaggtcac ccacgaccgc gggtggcagg tcgccgtcaa
     5881 cctcgtaggc gagctccacg ccctttgccg cggccgccgg ggcgatcacg tcgagcgcat
     5941 cctcgatgca gtcggccggg ctgaagggct cagcgtcaag atcgaccttg cccgcctcga
     6001 tcttcgagaa gtcgaggatg tcgttgatga tcgtcagcag cgcgtcaccc gatgagcgaa
     6061 tggtgtcggc atagtcgcgc tgctcgacgt tgagctccgt gtcgctcagg aggccgctca
     6121 tcccgatgat cgcgttcatc ggcgtgcgaa tctcgtggct catcgccgcg aggaatgcgc
     6181 tcttcgcctg gttggcggcg tcggccgcct cacgcgcctc gcgcagctcg gcgaagagct
     6241 gggcgttatc aatggcgatc gcggtgttct gcgacaggcc gaccaggaag tcgaggtcat
     6301 gcggcgtgaa tgcgcggtag ttgcccgtgc gccacacggc catcatgccg ttgacgcctg
     6361 cgcggccgat gaggggcgcg acgatcagcc gctcggtcgc catctggggc gtgccggcga
     6421 tctggatcgc ccgcttgtcg ctggcgacgt cgttgatgac ctcggcccgc gcctccgccg
     6481 ccagggtgcc gatgatgccc acgcccagct ccagcgtggt ctgcttgatc tgctccgcga
     6541 tggagcccac cgcggctgcg gcgcggaacc gtttgccgtc cggatcgcgc aggaagaccg
     6601 cggctgacgt gccgtcgagc aggtcggacg cctggttggc aatgcgcggc aggagcgtgc
     6661 ccagatcaag cgtcgccgag atctcccggg cgacgtcggc gagtgtcgac atctcggtcg
     6721 cgcggcgctg tgactcgcca tacaggagcg cgttctggat ggcgctgccg atgttcgcgg
     6781 cgagtgtggt cagcaggcgc acgtcgctct cgccaaagcg gccttcctgg gtcacgctct
     6841 ggacgctgac cgcgccgatt gccttgtcgc ccgcgaggat gggcacgccc agataggagc
     6901 gggcgatcgt gccgacgccg tggctggcca tgtcctcgtg gtcggacgcc ttgttgagca
     6961 gcaccggctg gcgcgcttgg atgatgcgcg acgtcatgcc ctggcccagc ggcagcggct
     7021 cctgcggctc gttcacgccg ttttcgacgt agaacgggaa ctcgatcatg ttggtgttcg
     7081 gatcgacgag cgccacgtag gcaatatccg cctggaaggt cgcggccatc tgcggacccg
     7141 caagatcaat cagcttctcg aggtccagct gcgaggcggt ggcctgaccc agctcgttga
     7201 cgatcgacag ctccgcggcg cgctggcgcg tctccgccac gaggcgcgcg ttatcgaggg
     7261 ccacgctaag gctcgccgcg agggtgctca gcagccggac atcggcctca gagaaggcac
     7321 tctttcgatc caggttctgc agcgatatcc gaccgaacac tcggtcgcct gacatcaacg
     7381 gcgcaaagac catcgattgc ggggcctcgc cctggatcgt cgcgggcacg ccgcgggcct
     7441 cctgccatgc caccgcatcg tcgatcacga cgggcttctt ttcggcgatc agctggcgcg
     7501 ccgtttgggt aatggcgatc ggctggtcgg ggaagcgcac gccccgctcc ttcgtatacg
     7561 ggaagctcac catctggcgg tcgaagtcga acagcccgat atcgaccacc tgggcatcga
     7621 aaatctcctg gatcttgtcg cccaccagct cgtacatggc ctgcatgtcc aggttggcgg
     7681 ctagaccctc ctggacgctg ttgatgatcg ccagctcggc cgcgcgctcg ttggtctccg
     7741 tgagcaggcg ctgcgtctca tcgaagagcc gcgcgttctc gagggccacg ctcaggctgc
     7801 cggccagcgt cgtcagcagc cgcaggtccg cttcgccgaa cgcgaactcg tgatccaggt
     7861 tctgaatgga gatgtctccg atgactttgc cgccacgcat cagcgccgca aagagcatcg
     7921 actttggcgg ttcgccgacg accacggact cgatgccctg ctcggcctcc cattccctta
     7981 tcccggagtt gaccaggagc ggctccgggt ggtcgatcag gtattgggca aaactgttga
     8041 gcggcagcac gacaacatct tgccgaaccc cgcgttcgac agcgaaatgc catgtcatct
     8101 cgccgcgctc gacatcgacg gtcgagatca ccatggcctg cgcatcgaag atctcggtga
     8161 tcttctcacc aacgagctcg tacatggctt gcatgtcgag cttctcggca agtccctgct
     8221 ggacgctgtt gatgatcgcc agctcggcgg cgcgctcgtt ggtctcagtc agcaggcgct
     8281 gcgtctcatc gaaaaggcgc gcgttctcaa gtgccacgct caggctgccg gcgagggttg
     8341 ccagcaggcg cacgtccgat tcgctgaaag cgtcataacg atctaggttc tggagcgaga
     8401 tgcggccgaa gagcttgccg ctgctgatta gcggcgcgaa gacgatcgac ttcgacggct
     8461 caccgataat cgagaacgtc aggccagtct cctcgaccca cttctcgatg ttgccgatgt
     8521 tcacgggcgc agggttcgtc ttctggtgct cgatgaacca cagggcgttg gggccgaagg
     8581 gtgcgggatc gtcgggcagc ttttggccct tctcgatcgt gtatggatag cgggtgagcc
     8641 ggttctcgaa gtcgtagatg ccgatgtcga gcacctgggc atcaaagatc tcgcggatct
     8701 tgtcgcccac gagctcgtac atcgattgca tgtcgagctt cgccgcgagg ctttcctgga
     8761 cgctgttgat gatcgccagc tctgccgcgc gctcgttcgt ctccgtcagc aggcgctgcg
     8821 tttcgtcgaa aaggcgcgcg ttctcaagtg ccacgctcag gctcgaggcg agcgtcgtca
     8881 gcagccggac gtcggcctcg ccgaaggcgt tctcgcggtc cagattctga agcgaaatga
     8941 tgccctggac ctcatcgccg ctcgcaaggg gcacccacag cgccgacctg gccatttcgc
     9001 cctcgagggc gccctcctgg cccagctcgc gaacgcgtgc ctcggcgtcc tcgttgatca
     9061 cgagtggctc tcgagtctcg gcgacacggc ggcgcgggcc tatcaacggg aatgttccgt
     9121 tttccagacg tctgccgcgt tcgacgccgt aggtgaacga catcgttt
//
LOCUS       DATBRX010000062        17845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_689423, whole genome shotgun sequence.
ACCESSION   DATBRX010000062 DATBRX010000000
VERSION     DATBRX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 17845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17845
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689423"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VIF08_02175"
     CDS             <1..264
                     /locus_tag="VIF08_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY8134819.1"
                     /translation="PGHTMSGFLFLLLQMLVTVLWLMILGRVLLSWINPRFDGPVGRF
                     LYETTEPLLAPIRRILPQTGMLDLSPLVLLLGLGFLMQLILRR"
     gene            279..545
                     /locus_tag="VIF08_02180"
     CDS             279..545
                     /locus_tag="VIF08_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY8134820.1"
                     /translation="MRVTPRAGRDEVVGVGVAGELLVKVRTAPADGAANVAMLRTIAA
                     AAGVAPSRLQLVRCATSRTKIVSIDGVTDAELRARWPGLLTRAG"
     gene            586..660
                     /locus_tag="VIF08_02185"
     tRNA            586..660
                     /locus_tag="VIF08_02185"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:618..620,aa:Val,seq:cac)
     gene            complement(727..2505)
                     /locus_tag="VIF08_02190"
     CDS             complement(727..2505)
                     /locus_tag="VIF08_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134821.1"
                     /translation="MAAVLVMLSAPVGPSVVRAATAPANDFFANAVVLTDSTLPYGPV
                     TIDTTNATTEANEPWEVSGCQGDYYGHLNHTVWYRYNATADGAIAVSTELSNFDTDIA
                     VYAGSSIGSLNQVACAHENGTTGHYLAFHAQAGLTYYIQIGGDSWDFGGPQFGSLTVA
                     IKPHVPGNDFFIDAVQLNSDSLPYGPVTLDTTRATNDPNEPWHLYNCVGTFDAFPYKT
                     VWYRFDATADEAISVSTDGSNFDTEIIVYVDSPATGFDNIACAHEHGVLGQHTSFQAH
                     AGLTYYIQIGGDSQDAMTDKFGSLHVEIKPHVLANDYFMEAVGINTASLPYGPVALDT
                     TDASIEAYEPAPWSCGDDYINGPTKTVWYRYDATTDGIVAVSTAGSNFDTVIAVYQGS
                     SMAGLTHVTCAHQTGVAGQYASFQTTAGATYWLQIGGDSGDTATAPYGPLVVSVGVAV
                     DQSDGLIKRGATAVIGNGIYNSHGQNQTLQASRRPRHSATFTINVQNDGRAADRFTVA
                     SSGPSIAGFTVKYFHGTTDITAAVVGGSFETPTLAPGSTYSITAKVKVTRTAAKRSST
                     YRLVTLRPEAAASGAFDAVGFIVKRG"
     gene            complement(2691..3356)
                     /locus_tag="VIF08_02195"
     CDS             complement(2691..3356)
                     /locus_tag="VIF08_02195"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEY8134822.1"
                     /translation="MAASAWMARARALRDSNPLAVAVLVAVNLVPLVGVLFWGWDVAT
                     ILIAYWLENGVIGLINIPKIMLAARDTPVIGPVIAGFFALHYGGFWIGHGIFVFVIVG
                     MATRGAFGFFMGVDPIGNVARDPQVPLIALLLLVSHGVSFFFNYMGRYEYLNTTPMKQ
                     MFQPYGRLVILHITIIVGAFFVIGLGQPVALVALLVILKTAVDLLFHLREHAKASQPV
                     TVI"
     gene            complement(3373..6057)
                     /gene="acnA"
                     /locus_tag="VIF08_02200"
     CDS             complement(3373..6057)
                     /gene="acnA"
                     /locus_tag="VIF08_02200"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY8134823.1"
                     /translation="MTQSTPLPDPFSARAELRVGHRAYIYYRLDRSGLADLAAAPMTV
                     KVLLENVIRNAGRGVVREEEARALAAWRPKLGSDAEVPFMPARVLMQDFTGVPAVVDL
                     AALRDAMAAIGGDPALVEPLVPADLVIDHSVQVDRYRTSDAFAFNVAREYERNGERYA
                     LLRWAQSAFAGLRVVPPGTGIVHQVNLEYLAAVVATRDDETGATVAFPDTLVGTDSHT
                     TMVNGLGVLGYGVGGIEAEAVLLGQPLYQPMPRVVGVRLHGDLPRGSTATDLVLVISN
                     MLRTHGVVGSFVEFAGDGLASLALADRATISNMAPEYGATAALFPIDDETLAYLRMTG
                     RRPEQVALVEAYAKENALWREPGPGPVFDETLELDLATVEPTVAGPRRPQDKVRLPDL
                     PANFHAAYPQTADAHPVAVAGNGHGPQTIDHGSVAIAAITSCTNTSNPTVMVGAGLLA
                     RNALARGLTVRPTVKTSLAPGSRAVTEYLQTAGLLKPLEELGFGVVGYGCTTCIGNSG
                     PLDAPVAEAIEGNELVVAAVLSGNRNFEGRIHPLARASYLASPPLVVAFALAGTVDID
                     LASQPLGIDRDGKPVLLADIWPTPEEIKRSIASSVSPEIFNRIYASVFDGDAQWRALP
                     VPVGSKRYEWDEASTYVARPPFMDGIQRDPAPVKDIVGARALAMLGDSVTTDHISPAG
                     SIPAWSPAGTWLQEHGVSPVDFNSYGARRGHHEVLMRGTFGNIRLRNALAEKEGPYTT
                     HQPSGEPMFIYDAAMRYAGEGVPLVVLAGKEYGSGSSRDWAAKGPALLGVRAVLAETY
                     ERIHRSNLVGMGVLPLQYLPGQNAESLGLTGHEEFSVVGLADGLAVRKHVTLRVRDAN
                     GTRDIEVIARIDGPVELDYYHQGGIMPAVLRRLAGVTN"
     gene            6372..7292
                     /locus_tag="VIF08_02205"
     CDS             6372..7292
                     /locus_tag="VIF08_02205"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY8134824.1"
                     /translation="MTDHQHGPDHQHADDGKPRIGFIGAGRVGTALAVAFSRAGWEVT
                     GVASRDEERRARFHSLVPTARAFADATNVLDEADLIFLTVPDDAIAATAESLHLYSGQ
                     ALVHTSGALPASVLSPAMAAGTSKASFHPLVAFADLEHALDALHGATVALDGDESLLP
                     VLADMAESVGARPVRLPEDGKAAYHAAAMMAAGGLVGLLDGIVAVARLAGLDEKTALA
                     VYTPLARQALANSERLGVDGALTGPFLRGDVGTVRGHLAVLRVSAPEALDLYIAVARR
                     ELAIARRRGDLAEEAATELESLLDDADGSD"
     gene            7343..7672
                     /locus_tag="VIF08_02210"
     CDS             7343..7672
                     /locus_tag="VIF08_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134825.1"
                     /translation="MRITSAGLKSGHPTTRFSRSPSTRRERQLGLRISTRPHMTAHPG
                     SHARVPLLTSLRQAARSASNVEPATSAPAASAPARITPSVSFTWTRPAGTGSRRPRPR
                     SAAPWRG"
     gene            7603..8148
                     /locus_tag="VIF08_02215"
     CDS             7603..8148
                     /locus_tag="VIF08_02215"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8134826.1"
                     /translation="MDATRRHWLTPAAAAQRRAVARLSEQARRKQARGGRTARATSCG
                     GILLRDVDGITEICLGRRQRSLGRGGMTWTLPKGTPNDGESIDQTALREVAEETGLEV
                     RIVAPVGAIEYFFTQGGERVHKTVHYFLMEAIGGSLEAHDHEFDEVRWMPVDEARRLM
                     TFETERQIVEEALPVAHQGAA"
     gene            8145..9296
                     /gene="gcvT"
                     /locus_tag="VIF08_02220"
     CDS             8145..9296
                     /gene="gcvT"
                     /locus_tag="VIF08_02220"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY8134827.1"
                     /translation="MIDHATDDSAASEDAAQTQMRRTALVDRHRALGARLIDFAGWEM
                     PVSYSGILEEHKAVRERVGLFDLSHMGEVWVSGPGAATGLATALVSDPTRLAEGRAHY
                     SMICNDDGGIIDDLIVYRVAAERFMVVPNASNREAVAAALRQRLDGHDAMLDDASLRT
                     SLVAVQGPRAAELLQPLTDVDLAPLKYYAIAAGHACGHPVLIARTGYTGEDGFELFVE
                     WDLANPVWDTLLDAGNSYDIQPCGLGSRDTLRLEAGMPLYGNELDLATNPFEAGLGRV
                     VKLDKPNDFVGRAALEKVAADGPRKQLVGLTMRGRNIARHGYPVSMPGDAEPTGIVTS
                     GAPSLTLGVPIAMAYVPPAHSAVGTMVEVAVRSTQAQADIVQLPFYKRS"
     gene            9302..9688
                     /gene="gcvH"
                     /locus_tag="VIF08_02225"
     CDS             9302..9688
                     /gene="gcvH"
                     /locus_tag="VIF08_02225"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY8134828.1"
                     /translation="MQVPDGLLYTQDHEWLRVEGDEAVVGITAYAATELGDIVFVEMP
                     ALGARFEPAQSFGVIESVKTASDLYAPAGGEIIAINEALHDKPELVNNDPYGEGWMIR
                     IKLATGAGAPDGLMEAPAYRALIEAA"
     gene            9709..11064
                     /gene="gcvPA"
                     /locus_tag="VIF08_02230"
     CDS             9709..11064
                     /gene="gcvPA"
                     /locus_tag="VIF08_02230"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEY8134829.1"
                     /translation="MTYGYGPNTAGDRERMLGAVGLESIDGLFSDIPASVRATGLDLP
                     AGLDELSLARRLETLAARNQVGLASFLGAGAYRHHIPATVDQILSRGEFYTAYTPYQP
                     EISQGTLQTIYEYQSLICQLTGLDVVSASHYDGGAATAEAALMTIRQTKRERVLVSRA
                     VHRHFVDTTRTYFQGGRLTLEELPTTAEGTTDLAALEQALADPDRPVAGVLIGQPNAF
                     GILEPMAQAAQLAHAAGALFVAVVEPVSLAVLAAPGDYEADIAVGEGQPLGIGLQYGG
                     PYLGLLAARSDLVRQVPGRLVGRTTDVDGRRAFVMTLRAREQDIRRDKAASNICTNQA
                     LCALASTVYIATLGPHGLRDVAAGGAAKAAELEGALAAFGVPRIHTGAYLNEFAVRVP
                     DARRIHAALLERGILAGLPLADWYPDDEALKDALLVCATEVTTSDDIAKFASALREVA
                     A"
     gene            11061..12611
                     /gene="gcvPB"
                     /locus_tag="VIF08_02235"
     CDS             11061..12611
                     /gene="gcvPB"
                     /locus_tag="VIF08_02235"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEY8134830.1"
                     /translation="MTATIERPEQETAQVPGPRQVVGPGLQPTLDELSRPGRATTKVP
                     HAPADALERIPPDHRRAAPLGLPELNEPEVIRHFVNLSQLNYSVDTGFYPLGSCTMKY
                     NPKINEWAARLPGFAALHPLAPDAVAQGTLELMWELEQWLAEISGMKAVTLQPAAGAH
                     GELTGILMIRAYHMARGDHERTEVIVPDSSHGTNPATASMAGFRTVTVKSTSEGQVDL
                     AALSAALGPHTAAVMLTNPSTLGLFERQIVELLDAVHAAGALAYMDGANMNAILGKFR
                     PGAAGFDVMHFNTHKTFSTPHGGGGPGAGPVAVNEKLVPFLPWPRVFRDGDAYRLEKP
                     GERPSSIGRVRSYQGSTGVLVRAYAYLLAHGGEGLVEVSEDAVLAANYLRHRLSDTFE
                     MPFAQVSKHEFVASAARLKDETGVRMLDIAKRLIDKGFHPPTIYFPLTVEECMLVEPT
                     ETESVETLDAFADALIEVAREAHDDPELVKSAPHDSPVRRLDEATAARQPDLRWRAMS
                     GRETPCPG"
     gene            12652..13647
                     /locus_tag="VIF08_02240"
     CDS             12652..13647
                     /locus_tag="VIF08_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8134831.1"
                     /translation="MTLASDTQAIGAGAPAQDTLNAAQGRSLREIVIARLRRDKVAMV
                     ALVILIIVYLLAIIGPFIGPMFGFNPFAYDPAAVATATGGPALPFGGISAQHPLGVEW
                     GTGRDIASQLLWGLRISLIVATASTVLVVSIGTIVGIIAGYGGGWTDMLLGRFMDLVL
                     AFPTLLVLIAMSAVLTQRLTDLGVPSGNPSRILYLVLAISAFGWPYLARIVRGQVLSL
                     REREFVEAAVAAGASRRRILFTEILPNLWAPILVYTTLALPAFIGLEAALSFLGVGIL
                     PPDVTFGVMLANSVKYFQTVPTYLFIPGLALVIIVVAFNLLGDSLRDALDPRATH"
     gene            13724..15784
                     /locus_tag="VIF08_02245"
     CDS             13724..15784
                     /locus_tag="VIF08_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8134832.1"
                     /translation="MAGGFPAQVFEEDPLMVITQGRNMRALALGVTTVMLLAACQGTN
                     LASPTPNASSTAPAPTGTGSTPTGTPVVTDAPEPTATPENLSGGTLFILAPTDLSNAG
                     EGIQDLDPQRTYTGEDLALLGATINRTLNNYVYSDDPAAASALVADAATDTGTASADA
                     KTWSFTVREGIKWQDGSALTCADFKFGASRVFATDLVGGGPSYALAYLDVATVDGFSP
                     VLDDAGNVTGAVAATKVAYAGPYQENIPNFFADEAMTQPVANDQAAFDAAVVCDGQTI
                     TYHLKQPIADFNYTVTLGLGAVPDPVDHPDVPLTLSGSEAYNDTPWSNGPYMIESFER
                     GVGGHLRFIRNPNYDPATDESGRMAYPDEIMMEQGLDPAVLDTRMITPTGDDRFAVSQ
                     GNVEPQNLETVYADPHTANPDFVGRAFSDFDPYVSYYTIRTDVVTNVKIRQAMAVALN
                     RDALRQNGGGEYVGDYADGFIKPNIGMDYAPTHLWEAAGPFGQDVPGTGDAALAQQLI
                     TDSGEAAPTLTWQYVPSDVSNRAAGIIQDSLQQAGFTINLSEITGPRYSCYFDLDCQT
                     EFQGTGWGPDWPNASTVIGPLFTTDGGWDISRVGQVNGENDPSSPDADYVDAVHANLG
                     QTDRQAQATEWQRLNALAGERMYAIPTFFGLTQTMAGDAVGNLYRWGPYGSWPYGILY
                     VKNQ"
     gene            15915..16895
                     /locus_tag="VIF08_02250"
     CDS             15915..16895
                     /locus_tag="VIF08_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8134833.1"
                     /translation="MVRRLVGMVILLLALTLAVFVMFNLIPGDPARLACGKSCSPDVL
                     DAIRHRLEYDLPMWEQYFRFLKGIFFGRVYGDNPLTQLECAAPCLGYSYLRHESVLRL
                     IGRSFPITFWLTLGGFIVWIVTGLIAGIYAALKRGRWQDRSLMGVALIGYSMPSFFIG
                     LILITFVVLEWHWLPFPKFAPPEQNPARFLQTMILPWIVIAVLNAAFYVRLTRNQVLE
                     TFSEDYVRTARAKGLPERTVVVKHALRAGLTPIVTAAGLDIAYLLGGAIITESIFTLQ
                     GLGQVSIASIRDFDLPIITAITMISAAFIIVANLVVDLLYAVLDPRVKVG"
     gene            16896..>17845
                     /locus_tag="VIF08_02255"
     CDS             16896..>17845
                     /locus_tag="VIF08_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8134834.1"
                     /translation="MIGRLNKMAPGGAVEVPEAAGDRPFLEVKDLFVKFPTEDGLVHA
                     VDGVSFTLERGKTLAIVGESGSGKSVTAQAIMGLLDRRQAKVSGEIWLDRLNLLALKP
                     AQVQALRGERMAMIFQDPMSSLHPFYRVGDQIVEAIQAHRSVSKSAARQETLDMLKRV
                     GIPAAARRIDAYPHQLSGGMRQRVMIAMALINSPELLIADEPTTALDVTVQAQILELI
                     RQLQAEFGTTVIMITHDLGIVADVADEVAVMYGARIMERASTQEIYEHPEMPYTLGLL
                     SSIPRMDRDVTGRLEPIKGNPPSAVRLPSGCVFEPRCPYSG"
BASE COUNT         2930 a         6039 c         5905 g         2971 t
ORIGIN      
        1 cccggtcaca cgatgagcgg cttcctgttt ctccttctcc agatgctcgt cacggtgctg
       61 tggctgatga tcctcgggcg ggtgctgctc tcatggatca atccgcgctt cgacggtccg
      121 gttggccgct tcctgtatga aacgaccgag ccgctgctcg cgccgatccg gcgcatcctg
      181 ccccagacgg gcatgctcga tctgtcgccc ctggtgttgc tgctcggtct gggcttcctg
      241 atgcagctga tcctgcgccg ctgagcgtca ccttcggcgt gcgcgtcacg ccacgtgccg
      301 gtcgagatga ggtcgttggc gtgggtgtgg ccggcgagct cctggtcaag gttcgcacgg
      361 cacccgcgga cggcgccgcg aacgtggcca tgctgcgaac cattgctgct gccgccggcg
      421 tcgcgccatc gcggctgcag ctggtgcgct gcgcgacgtc gcgcaccaag atcgtgtcga
      481 ttgacggcgt gacggacgcc gagctgcggg cgcgctggcc gggtttgctg acaagggctg
      541 gctgagcgcc gataatcggc ctgccgcgcg atgcggcgtc cacgggggcg attggctcag
      601 cggtagagca ctcggttcac atccgagggg tcactggttc gaatccagta tcgcccacca
      661 aagtcacccc acgcgagagc ggagccgtta cctcccgcat tgcgttcaag gcgtgggcac
      721 cgcaggctaa ccgcgcttga cgatgaatcc gaccgcgtcg aatgcgcccg acgctgcagc
      781 ctcgggcctt aacgtcacga ggcggtatgt gctcgatcgc ttggctgctg tgcgcgtcac
      841 cttgaccttg gccgtgatcg agtaggtgct gccgggcgca agggtcggcg tctcgaagct
      901 cccgccgacc acagcggccg tgatgtcggt cgtgccgtgg aagtacttga cggtgaagcc
      961 cgcgatactg gggccgctcg atgcgaccgt gaagcgatca gccgccctgc cgtcgttctg
     1021 gacgttgata gtgaaggtcg ccgaatgccg tgggcgccgc gacgcctgga gcgtttggtt
     1081 ctggccatga gagttgtaga tgccgttgcc gataaccgcg gttgcgcccc gcttgatcag
     1141 accatcggac tggtccacag ctacgccgac agacaccacc agcggtccgt agggggccgt
     1201 cgcggtgtcg ccagagtcgc cgccgatctg cagccagtag gtcgcgccgg cagttgtctg
     1261 gaaggaggcg tactgacccg caacgcccgt ttggtgagca caagtcacgt gggtcaggcc
     1321 ggccatcgaa ctgccttgat agacggcgat cacggtatcg aagttcgatc ccgcggtact
     1381 gacagcgacg atcccgtctg tggttgcgtc gtaccgatac cagaccgttt tggtcggccc
     1441 atttatgtag tcgtcgccac aggaccacgg ggcaggctcg taagcctcga tcgacgcgtc
     1501 cgtcgtgtcg agggccactg ggccgtacgg caggctggcg gtattgatcc cgaccgcctc
     1561 catgaagtag tcattggcca ggacgtgcgg ctttatctca acgtggagcg atccgaattt
     1621 gtccgtcatc gcatcctggc tgtctccgcc gatttggatg tagtaggtga gcccggcgtg
     1681 agcctggaaa gaagtgtgct ggcccaggac tccatgctcg tgcgcacatg cgatgttgtc
     1741 gaacccggtt gccggggaat ccacgtaaac gatgatctcc gtgtcgaagt tcgagccgtc
     1801 ggtgctgaca ctgatcgcct cgtcggccgt cgcgtcgaac cggtaccaga cggtcttgta
     1861 gggaaatgcg tcgaaggtac cgacgcagtt gtagagatgc caaggctcgt tggggtcgtt
     1921 cgtcgctcgg gtcgtgtcga gcgtgaccgg gccatagggc aggctgtcgc tgttgagctg
     1981 gacggcgtcg atgaagaagt cgttgcctgg gacgtgcggc ttgatcgcca ccgttaggga
     2041 gccgaattgc ggaccgccga agtcccagct gtcccctccg atctggatgt aatacgtcag
     2101 gcccgcctga gcgtggaagg ccaggtagtg gcccgttgtc ccattctcgt gggcgcacgc
     2161 cacctgattg aggctgccga tcgaactccc cgcatacacc gcgatgtccg tgtcgaaatt
     2221 cgagagttcg gtgctgacgg cgatcgcacc gtcggccgtc gcgttgtagc gataccagac
     2281 ggtgtggttg agatgaccgt agtagtcccc ttggcagcca gagacctccc atggctcgtt
     2341 cgcctcagtg gtggcgttgg tggtatcgat cgtgaccggg ccgtacggca aggtggagtc
     2401 ggtcagcact acggcattcg cgaagaagtc gttggcaggc gctgtggccg cgcggactac
     2461 agaaggtcca accggcgcag agagcatgac aaggacggcc gccattgctg aaacgcgttg
     2521 cgcgcgcgct ttaatggcgg agacctgtcg ggtcagagac tgcctgcccg aaaccatctg
     2581 acttccccct cgtgaactgc tttccgaggc tcgtcaaggt gcctccggac cggcgggcac
     2641 ttgcccagcg gacattctct catggcgtca agagccgacc ggccagcagc ctagatgaca
     2701 gtcacgggct gtgacgcctt cgcgtgctcg cgcaggtgga agagcagatc aacggcggtc
     2761 ttcaggatca ccagcagcgc caccagcgcc accggctggc caaggccgat cacgaagaac
     2821 gcgccgacga tgatcgtgat gtgcaggatc accagtcgtc cgtaaggctg gaacatctgc
     2881 ttcatcgggg tcgtgttcag gtactcgtat cggcccatgt agttgaagaa gaagctgacc
     2941 ccgtgactca ccagcagtag cagtgcgata agggggacct gcgggtcgcg ggccacgttg
     3001 ccgatcgggt cgacgcccat gaagaagccg aacgcgccgc gcgtggccat gccgacgatg
     3061 acgaagacga agatgccgtg gccgatccag aagccgccgt agtgcagcgc gaagaaaccg
     3121 gcgatcaccg gtccgatcac cggggtgtcg cgcgcggcga gcatgatctt cgggatgttg
     3181 atcaggccga tcacgccgtt ttcgagccag tacgcgatga ggatcgttgc gacgtcccag
     3241 ccccagaaca ggacgccgac cagcggcacc aggttgaccg ccacgaggac cgccaccgca
     3301 agcgggttcg agtcgcgcaa tgcccgtgcg cgggccatcc aggctgaggc tgccatcccc
     3361 cgattttgac gcctagttgg tcacccccgc cagccgccgc agcacagccg gcatgatgcc
     3421 gccctggtgg taatagtcga gctcgaccgg accgtcgatg cgcgcgatga cctcgatgtc
     3481 ccgcgtcccg ttcgcgtcac gaacccggag cgtcacgtgc ttgcggactg cgagtccatc
     3541 ggccaacccg acgacgctga actcctcgtg gccggtcagg ccgagcgact ctgcattctg
     3601 gcccggcagg tactgcagcg gcagcacgcc catgccgacg aggttcgagc gatggatgcg
     3661 ctcgtaggtc tcggcgagga cggcgcggac acccagcagt gccgggccct tggccgccca
     3721 gtcacgcgac gagccgctgc cgtactcctt gcccgcgagc accacgagtg gcacgccctc
     3781 gccggcgtag cgcatggccg cgtcgtagat gaacatcggc tcgccgctcg gctggtgggt
     3841 ggtgtacggg ccctccttct ccgcgagggc gttgcgcagg cggatgttgc cgaacgtgcc
     3901 gcgcatcagc acctcatggt gaccgcgccg cgccccgtag gagttgaagt cgacaggcga
     3961 tacgccgtgc tcctgcagcc acgtgcccgc gggtgaccag gccgggatgg agccggcggg
     4021 cgagatgtga tcggtcgtga ccgagtcgcc cagcattgcc aacgcgcgcg cgccgacgat
     4081 gtctttcacg ggcgctgggt cgcgctgaat gccgtccatg aagggcgggc gcgcaacata
     4141 ggtcgaggcc tcgtcccact cgtagcgctt cgagccaacc ggcacgggca gtgcgcgcca
     4201 ctgcgcgtcg ccgtcgaaga cgctggcgta gatgcggttg aagatctccg gcgaaaccga
     4261 cgatgcgatg gagcgcttga tctcctcggg cgtgggccag atgtcggcca gcagaaccgg
     4321 cttgccatcg cggtcaatgc ccaggggctg ggacgccagg tcgatgtcga ccgtccccgc
     4381 cagagcgaac gccacgacca ggggcggcga ggccaggtac gaggcgcgcg ccaacggatg
     4441 aatgcggccc tcgaagttgc ggttgccgct cagcaccgcg gcgaccacca gctcgttgcc
     4501 ctcgatcgcc tccgcgaccg gcgcgtcgag cggaccgctg ttgccgatgc acgtggtgca
     4561 gccatagccg accaccccga agcccagctc ctccagcggc ttgagcagac ccgcggtctg
     4621 aaggtattcg gtcacggcgc gtgaccctgg cgcgagcgag gtcttgacgg tgggcctgac
     4681 cgtcaggccg cgagcgagcg cgttgcgagc gagcaggccg gcgccgacca tcacggtcgg
     4741 gttggaggtg ttggtgcagg acgtgatcgc cgcgatcgcc acggaaccgt gatcgatcgt
     4801 ctgggggcca tggccattgc cggcgaccgc gactggatgt gcgtcggccg tctgcgggta
     4861 ggcggcatgg aagttcgcgg gcaggtcggg cagtcggacc ttgtcctgcg gccggcgtgg
     4921 cccggcgacg gtcggttcga ccgtggccaa atcgagctcg agcgtctcgt cgaacaccgg
     4981 cccggggccg ggctcgcgcc acagcgcgtt ctccttggcg taggcctcga ccagcgcgac
     5041 ttgctcgggc cgccgaccgg tcatgcgcag atacgcgagc gtctcgtcat cgatcgggaa
     5101 gagcgccgcg gtcgcgccgt attcgggcgc catgttggag atcgtcgccc ggtcggccag
     5161 cgccagtgac gccaggccgt cgcccgcgaa ctcgacgaac gacccgacca cgccgtgcgt
     5221 ccgcagcatg ttcgagatga ccagcacgag gtcggtcgcg gtcgagccgc gcggcaggtc
     5281 gccgtgcagt cgcacaccga cgacgcgcgg catgggctgg tagagcggct gacccagcag
     5341 cactgcctct gcctcgatgc cgccgacgcc atagcccagc acgcccagcc cgttgaccat
     5401 cgtcgtgtgt gagtcggtgc cgaccagagt gtcggggaag gcgacggtcg cgccggtctc
     5461 atcgtcgcgg gtcgcgacaa ccgccgcgag gtactcgagg ttcacctggt ggacgatgcc
     5521 cgtgccgggc ggcacgacgc gcaggccggc gaaggcactc tgcgcccacc gcaggagcgc
     5581 gtatcgctcg ccgttgcgct cgtattcgcg cgcgacgttg aatgcgaacg cgtcagatgt
     5641 gcgatagcga tcaacctgga ccgagtggtc aatgaccagg tccgccggga cgagcggctc
     5701 aaccagcgcc gggtcgccgc cgatcgcggc catcgcgtcg cgcaacgccg cgaggtccac
     5761 taccgccggc accccagtga agtcctgcat caggacgcgc gccggcatga agggcacctc
     5821 tgcgtcgctg cccagcttgg gtcgccacgc ggcaagtgcg cgcgcctctt cctcgcggac
     5881 gacgccgcgc ccggcgttgc gaatgacgtt ctcgagcagg accttgaccg tcatcggcgc
     5941 ggcggccagg tcggccaggc cgctgcggtc gagccggtaa tagatgtacg cgcgatgccc
     6001 gacgcgcagc tcggcgcgtg ccgaaaaagg atcgggaagg ggagtggact gagtcatcgg
     6061 cgacgggtct ccgagtcccc gcaagcattt ggcggggctg agcggcacct ggcgggagtg
     6121 cccgccgcgg gtcgcgatgt gtcgacctgc cgagtatagg gcacgggacg cggttcacaa
     6181 aggttccgtc gctgtcttac gccccacgtc cgaatgcgcc gaaacccaac cgcgaccggc
     6241 ctcctgagag cccaaatccg gttgtttctg tcgtcccacg cacaattcgg ccggatctcg
     6301 cccgcttggt gccggatatc ggtgtattcg gtcgtgccac gtcaggtcgc caaggctgga
     6361 caatggcgga gatgaccgac caccagcatg gccccgacca tcagcacgct gacgacggca
     6421 agccgcgcat cggcttcatc ggcgccggtc gagttggcac ggcattggcc gtcgcgttca
     6481 gcagagcggg ctgggaggtc acgggcgtgg ccagtcggga cgaggagcgc cgggcgcgct
     6541 ttcactcact tgtgccgacg gcgcgcgcat ttgcggacgc gaccaatgtc ctcgacgagg
     6601 ccgacctcat cttcctgacg gtgcctgacg acgcgatcgc cgcgacggca gagagcctgc
     6661 atctctacag cggccaggca ctggtccata cgtcgggcgc cctgcccgcg tcggtcctgt
     6721 cacccgcgat ggccgcgggc acgagcaagg cgagcttcca tccactggtc gcgttcgccg
     6781 atctcgagca cgccctggat gcgctgcacg gcgcgacggt ggcgctcgat ggcgacgagt
     6841 cgctgctgcc ggtgctcgcg gacatggccg agtcggtcgg ggcgcggccg gtgcgcctgc
     6901 ccgaagatgg caaggcggcc taccacgcgg ccgcgatgat ggctgcgggc ggcctggtcg
     6961 gattgctcga cggcatcgtc gccgtcgcgc gcctcgccgg gctcgacgag aagacggcgc
     7021 tcgcggtcta cacaccgctc gcccgccagg cgctggcgaa cagtgagcgc ctgggagttg
     7081 atggtgcgtt aaccgggccg ttcctgcgcg gcgatgtcgg gacggtgcgc ggccatctcg
     7141 cggtcctgcg cgtgtccgcg cccgaggcac tcgatctcta catagcggtt gcgcgccgcg
     7201 agctggccat cgcgcgccgg cgcggcgatc tggctgaaga ggccgcgacc gagctcgagt
     7261 cgctcctcga cgatgcggac gggtccgact gaactttcgc gccaattgca ggcggcgggc
     7321 caaccgctaa cattcgaacg cgatgcgcat cacatccgcc ggattaaaga gcggccaccc
     7381 gaccacccga ttcagccgct cccccagcac ccggcgcgag cgccagctgg gcctccgaat
     7441 ctcgactcgg ccgcacatga cggcccaccc cggaagtcac gcgcgagtgc cgctgctgac
     7501 ctcgctgcgc caggccgcgc gcagcgcctc caacgtcgaa ccagcgacca gcgcgcctgc
     7561 cgcgtcggca ccggcacgca tcacaccgag cgtctcattc acatggacgc gacccgccgg
     7621 cactggctca cgccggccgc ggccgcgcag cgccgcgccg tggcgcggct gagcgagcag
     7681 gcgcggcgca agcaagctcg cggcggcagg actgcccgcg cgacatcgtg cggcggcatc
     7741 ctgttgcgcg acgtcgacgg catcacggag atctgcctgg gccggcgtca acgctcgctt
     7801 ggacgaggcg gtatgacgtg gacgctgccc aagggcacgc ccaatgatgg cgagagcatt
     7861 gaccagaccg cactgcgcga ggtcgccgag gagacgggcc tggaggtgcg catcgtagct
     7921 cccgtcggcg cgatcgagta cttcttcacc cagggtggcg agcgcgtcca caagaccgtc
     7981 cactacttcc tgatggaagc gatcggtggc agccttgagg cccacgacca cgagttcgat
     8041 gaggtgcgct ggatgccggt cgatgaagcg cgccggctga tgaccttcga gaccgagcgc
     8101 cagatcgtcg aagaggcgct gcccgtcgcg caccagggtg ccgcttgatc gaccacgcga
     8161 cagacgattc ggccgcgagc gaggacgcgg cgcaaacgca gatgcgccgg acggcgctcg
     8221 tcgatcgcca tcgcgcgctg ggtgcgcgcc tgatcgactt cgccggctgg gagatgccgg
     8281 tgtcgtactc gggcatcctc gaggagcaca aggctgtgcg cgagcgcgtc ggcctgttcg
     8341 acctgtcgca catgggcgag gtctgggtga gcggccccgg cgcggcaaca ggacttgcca
     8401 cggcgctcgt cagtgacccg acgcgcctcg ccgaggggcg cgcccactac tccatgatct
     8461 gcaatgacga cggcggcatc atcgacgact tgatcgtcta tcgcgtcgcc gcggagcgct
     8521 tcatggtcgt gcccaacgcg tccaaccgcg aggcggtcgc ggccgccctg cgccaaagac
     8581 tcgacggtca tgacgccatg ctcgatgacg cgtcgctgcg cacctcgctc gttgccgtcc
     8641 aagggccacg agccgcggag ctgctgcagc cgctgaccga tgtcgacctg gcgccgctca
     8701 agtactacgc catcgccgcg ggtcacgcgt gtggccatcc ggtgctcatc gcgcgcaccg
     8761 gctatacggg cgaggatggc ttcgagctct tcgtcgaatg ggacctcgcc aatccggtct
     8821 gggacacgct ccttgatgcc ggcaacagct acgacatcca gccgtgcggc ctggggtcgc
     8881 gcgacacgct gcgcctggaa gcgggcatgc cgctctacgg caacgagctc gaccttgcga
     8941 ccaatccatt cgaggccggc cttggccgcg tcgtcaagct cgacaagcca aacgacttcg
     9001 tcggccgcgc cgcgctcgag aaggtcgccg cggacggccc gaggaagcag ctcgtgggcc
     9061 tgacgatgcg cggccgcaac atcgcccgtc atggctaccc cgtgtccatg ccgggcgacg
     9121 ccgaacccac aggcatcgtc accagcggtg cgccgtcact caccctgggc gtgcccatcg
     9181 ccatggccta cgtgcccccg gcgcacagcg ctgtcggtac catggtcgag gtcgccgtca
     9241 ggtcgaccca ggcccaggcc gacatcgtcc aactgccctt ctacaagcgg agctagccac
     9301 cgtgcaggta cccgacggac tcctctacac ccaggaccac gagtggctgc gcgtcgaggg
     9361 cgacgaggcg gtggtcggca tcaccgcgta cgccgcgacc gagctgggcg acatcgtctt
     9421 tgtcgagatg cccgccctgg gtgcgcgctt cgagccggct cagtctttcg gcgtgatcga
     9481 atcggtcaag acagcgtccg acctgtacgc gcctgccggg ggcgagatca tcgccatcaa
     9541 tgaggcactg cacgacaagc cagagctggt gaacaacgat ccatacggcg aaggctggat
     9601 gatccgaatc aagctcgcca ccggcgccgg cgcgccggac gggctgatgg aagcgccggc
     9661 ttaccgcgcg ctgatcgagg ccgcctgatc tcgaacagat aagaaaacat gacctacggc
     9721 tacggaccca acaccgcggg cgatcgcgag cgcatgctcg gcgccgttgg cctcgaatcg
     9781 atcgacggcc tgttctccga cattcccgcg tctgtccggg ccacggggct tgacctgccc
     9841 gccgggctcg acgagctgtc gctcgcgcgg cggctcgaga cactcgcagc gcgcaaccag
     9901 gttgggctcg cgagcttcct gggcgcgggc gcgtatcgcc atcacatacc tgcgaccgtc
     9961 gaccagatcc tgtcgcgcgg cgagttctac accgcgtaca cgccgtatca gcccgagatc
    10021 agccagggca cgctgcagac gatctacgag taccagtcgc tcatctgtca gctgaccggg
    10081 ctcgatgtcg tatcggcgtc gcactacgac ggcggcgcag cgacggcgga agcggccctg
    10141 atgaccattc gccagaccaa gcgcgagcgc gtccttgtga gccgcgcggt ccaccgccac
    10201 ttcgtcgaca cgacgcggac ctatttccag ggcggccgcc tgaccctcga agagctgccg
    10261 accacagcgg agggtacgac cgacctggcc gccctggagc aggcgctggc cgaccccgac
    10321 cgacccgtcg ccggcgtgct gatcggccag cccaacgcct tcggaatcct cgagccgatg
    10381 gcgcaggcgg cgcaactggc acacgctgcg ggcgcgttgt ttgtcgcggt cgtcgagccg
    10441 gtgtcgctgg cggtcctggc cgcgcccggc gactacgagg ctgacatcgc ggtcggcgag
    10501 ggccagccgc tgggcatcgg gctgcagtac ggcggtccgt acctgggcct tctcgcagct
    10561 cggtcggatc tggtccgcca ggtgccgggc cggttggtcg gacggacgac tgatgtcgac
    10621 ggccggcggg cgttcgtgat gacgctgcgc gcgcgcgaac aggacatcag gcgcgacaag
    10681 gcggcgagca acatctgcac caaccaggcg ctgtgcgcgc tcgcgtcgac ggtctacatc
    10741 gccaccctgg ggccgcacgg cctgcgcgac gtcgccgcgg gtggcgcggc caaggccgct
    10801 gagctggagg gtgcgctagc agcgttcggc gtgccgcgca ttcacaccgg cgcgtacctc
    10861 aatgaattcg ccgttcgcgt gcccgatgcg cgacggatcc atgcggcgtt gctcgagcgc
    10921 ggcattctcg ccggcctgcc gctcgccgac tggtaccccg acgacgaagc tctgaaggac
    10981 gcgttgctcg tgtgcgcgac cgaggtcacg acgagcgacg acatcgcgaa attcgccagc
    11041 gcactgcgcg aggtggccgc atgaccgcca ccatcgagcg cccggagcag gagacagcgc
    11101 aggtacccgg tccgcgccag gtggtcgggc cgggccttca gcccacgctc gacgagctct
    11161 cgcggcccgg tcgcgcgacg accaaggtgc cgcacgcgcc ggccgacgcg ctcgagcgca
    11221 ttccgcccga tcaccgccgc gccgcgccgc ttggcctgcc ggagctgaac gagcctgagg
    11281 tcatccgcca cttcgtgaac ctctcgcagc tcaactacag cgtcgacacg ggcttctacc
    11341 cgctcggctc gtgcacgatg aagtacaacc ccaagatcaa cgagtgggcc gcgcggctgc
    11401 caggtttcgc ggcgctccat ccgctcgcgc cagatgccgt cgcgcagggc acgctcgagt
    11461 tgatgtggga gctggagcaa tggctcgccg agatcagcgg catgaaggcg gtcacgctcc
    11521 agccggctgc cggcgcgcac ggcgagctga cggggatctt gatgatccgg gcgtaccaca
    11581 tggcgcgcgg cgatcacgag cggacagagg tgatcgtgcc cgattcgtcg cacggcacca
    11641 accccgcgac ggcatcgatg gccggtttca ggaccgtcac cgtcaagtcg acctcggagg
    11701 gccaggttga cctcgccgcg ctcagcgctg cgctgggtcc gcacacggcc gccgtgatgc
    11761 tcaccaatcc gtcgacgctg ggcctcttcg agcgccagat cgtcgagctc cttgacgccg
    11821 tccacgccgc cggtgcgctg gcgtacatgg atggcgccaa catgaacgcg atcctcggca
    11881 agttcaggcc cggcgccgcc ggcttcgacg tcatgcactt caacacccac aagaccttct
    11941 cgacaccgca cggcggcggt ggtcccggcg ccgggccggt ggccgtcaac gagaagctcg
    12001 tgccgttcct gccgtggccg cgcgtgttcc gtgacggcga cgcctatcgc ctcgagaagc
    12061 caggggagcg gccgagctcg atcggccgcg tccgctcgta ccagggcagc accggcgtgc
    12121 tcgtgcgcgc gtacgcgtac ctgctggcgc atggtgggga ggggctcgtc gaggtcagcg
    12181 aggacgcggt gctcgcagcg aactatctgc gccaccgcct gagcgacacc ttcgagatgc
    12241 cgttcgccca ggtcagcaag cacgagttcg tggccagcgc cgcgcggctc aaggacgaga
    12301 ccggcgtgcg catgctcgac atcgccaagc ggctgatcga caagggcttc cacccgccga
    12361 ccatctactt cccgctcacc gtcgaggagt gcatgctcgt cgagccgacg gagaccgaat
    12421 cggtcgagac gctcgacgcc ttcgctgacg cgctcatcga ggtggcgcgc gaggcgcacg
    12481 acgatccgga gctggtcaag tcggcgccgc acgactcacc ggtgcgccgc ctcgatgagg
    12541 cgacagccgc ccggcagccg gatctacgct ggcgagcgat gtccggccgt gaaacaccct
    12601 gccccggcta gttgcatcaa atcgccacat ggcgtgctac cctcctgaac catgacgctg
    12661 gcatcggaca cccaggcgat cggcgctgga gcgccggcac aggacaccct caatgctgcg
    12721 cagggccgga gcctgcgtga gatcgtcatc gcgcgcctgc gccgcgacaa ggtcgcgatg
    12781 gtcgccctgg tcatcctgat catcgtgtac ctgctggcga tcatcggacc gttcatcggt
    12841 ccaatgttcg gattcaaccc attcgcctac gaccccgcgg cggtcgccac ggcaacggga
    12901 gggccggcgc tgccattcgg cggcatcagc gcacagcacc cgctcggcgt ggagtggggc
    12961 accggacgtg acattgcctc gcagctgctg tggggtctgc gcatctcact gatcgtcgcc
    13021 acggcgtcaa ccgtgctcgt ggtcagcatc ggcacgatcg tgggcatcat cgccggctat
    13081 ggcggcggat ggaccgacat gctgctcggg cgcttcatgg acctggtcct ggccttcccg
    13141 acgctgcttg ttctgattgc catgtctgcc gtgctgacgc agcgcctgac tgacctgggc
    13201 gtgccgtccg gcaacccctc gcgcatcctc tatctggttc tggcgatcag tgcgtttggc
    13261 tggccgtacc tcgcgcgcat cgtgcgcggc caggtgctga gcctgcgcga gcgcgagttc
    13321 gtcgaggccg ctgtggctgc gggtgcgagc cgacggcgca tcctcttcac ggagatcctg
    13381 cccaacctgt gggcacccat cctcgtctac acgacgctcg ccctgccggc gttcatcggc
    13441 ctcgaagcag cgctgtcgtt cctgggcgtg ggcatcctgc cgccggacgt gacgttcggc
    13501 gtgatgcttg ccaactcggt gaagtatttc cagaccgtgc cgacgtacct tttcatcccg
    13561 ggcctcgcgc tggtcatcat cgtggtcgcg ttcaacctgc tgggagactc gctgcgcgac
    13621 gctctcgatc cgcgcgcgac ccactgagag gcccaataga ggtctattaa cttacctaga
    13681 tctagcgccc gctccgagat cgtgcgttgt atgatctggc ggcttggccg ggggcttccc
    13741 ggcgcaggtc tttgaggagg acccactaat ggtgataacc caggggcgaa acatgcgcgc
    13801 cctggcgctt ggcgttacca cggtgatgct gttggcggcc tgtcaaggca ccaacctggc
    13861 atctccaacg ccaaacgcaa gctctactgc accggcacca acaggtaccg gtagcactcc
    13921 aacgggaacc ccggtcgtaa ccgacgcccc ggagccgacg gcgaccccgg agaatctctc
    13981 cggcggcacg ctgttcatcc tggcgccgac cgatctatcg aacgctggcg agggtatcca
    14041 ggacctggac ccgcagcgca cctataccgg tgaagacctg gcgctccttg gcgccacgat
    14101 caaccgaacc ctgaacaact acgtctactc agacgatcct gcggcagcct ccgcgctcgt
    14161 tgccgatgcg gcgacggaca cgggcaccgc gagcgctgac gcgaagacgt ggagcttcac
    14221 ggttcgcgaa ggcatcaagt ggcaggacgg ctcggccctt acctgcgcgg acttcaagtt
    14281 cggcgcctcg cgcgtgttcg cgaccgatct cgtcggtggc ggcccgtcgt atgcgcttgc
    14341 gtaccttgac gttgccacag tcgatggctt ctccccggtg ctcgatgatg cgggcaacgt
    14401 gacgggtgca gtggcggcca ccaaggtcgc ttacgccggc ccgtaccagg agaacatccc
    14461 caacttcttc gcggacgagg cgatgaccca gccggtcgcc aacgaccagg ccgcgttcga
    14521 tgccgccgtc gtgtgcgatg gccagaccat cacctatcac ctgaagcagc ccatcgcgga
    14581 cttcaactac acggtgacgc tgggcctggg cgctgtcccg gatccggtcg accaccccga
    14641 tgttcccctc accctctccg gcagtgaggc ctacaacgac acgccctggt ccaacggccc
    14701 gtacatgatc gagtcgttcg agcgcggcgt cggtggccac ctgcgcttta tccgcaaccc
    14761 gaactacgat ccggcgaccg acgagagcgg ccgcatggcg tatcccgacg agatcatgat
    14821 ggagcagggc cttgacccgg ctgtcctcga cacgcgcatg atcaccccca cgggtgatga
    14881 ccgcttcgcg gtctcgcagg gcaatgtcga gccgcagaac ctggagaccg tgtacgcgga
    14941 tccgcacacc gccaacccgg acttcgtggg tcgtgccttt agcgacttcg acccgtacgt
    15001 gtcgtattac acgatccgca cggacgtggt caccaacgtc aagatccgcc aggcgatggc
    15061 ggttgcgctc aatcgtgacg cgctccgtca gaacggtggc ggcgagtacg tcggcgacta
    15121 tgccgacgga ttcatcaagc ccaacatcgg catggactac gcaccgaccc acctgtggga
    15181 agccgctggc cccttcggcc aggacgtccc gggcacaggt gatgcggcgt tggcccagca
    15241 gctgatcacc gactccggtg aagcagcacc gacgcttacc tggcagtacg tcccgagcga
    15301 cgtctccaac agggcagcgg gcatcatcca ggattcgctc cagcaggccg gcttcacgat
    15361 caacttgagt gagatcaccg gtccgcgcta ctcctgctac ttcgatctgg attgccagac
    15421 ggagttccag ggcacgggct ggggccccga ctggccgaat gcctcgacgg tgatcgggcc
    15481 gctgttcacc acggacggcg gttgggacat ctcccgagtc gggcaggtca atggcgagaa
    15541 cgacccgtcg tctcctgacg cggactacgt cgatgccgtt cacgccaacc tcggtcagac
    15601 cgaccgtcag gcacaggcga ccgagtggca gcgcctcaac gcgctggcag gcgagcgcat
    15661 gtacgccatc ccgacgttct tcggcctcac ccagaccatg gccggcgacg ccgtgggcaa
    15721 tctgtatcgt tggggtccgt acggctcatg gccgtatggc atcctgtacg tcaaaaacca
    15781 gtaaggtctc cttcgggaac cagactgtga acaccgggga agcggcgcga gccgcttccc
    15841 cggtgtctct atccagcgaa atccggctgc tgttctagcg acacacctgg agggcaggtc
    15901 ccgttttcgg ttacatcgtc cgacgtctcg tgggcatggt gatcctgctg cttgcgctga
    15961 cgctggccgt attcgtgatg ttcaacttga taccgggtga tccggcgcgc cttgcctgcg
    16021 gcaagagctg ctcgcccgac gtcctcgacg ccatacgcca tcggctcgag tacgacttac
    16081 cgatgtggga gcagtacttc agatttctga agggcatctt cttcggtcgc gtttacgggg
    16141 ataacccact cacccaactc gagtgtgccg cgccgtgcct gggctattcg tatctgcgcc
    16201 atgaatcagt gctccgcctc atcggacgca gcttcccgat caccttctgg ctgactctgg
    16261 gcgggttcat cgtgtggatc gtcaccggct tgatcgccgg catctacgcc gccctcaagc
    16321 gcggccgctg gcaggaccga agcctcatgg gcgtcgccct gatcggctac tcgatgccat
    16381 cgttcttcat cggcctgatc ctgatcacct tcgtggtcct cgagtggcat tggctgccat
    16441 tcccgaagtt cgcaccacca gagcaaaatc ccgcgcgctt cttgcagacg atgatcctgc
    16501 cgtggatcgt gatcgccgtg ctcaacgctg cgttctacgt gcgtctcacg cgcaaccagg
    16561 tgcttgagac gttcagcgag gattacgtgc gcacggcacg cgccaagggg ctgcccgagc
    16621 ggacggtggt ggtcaaacac gctctgcgcg ccggtttgac gcccatcgtc accgccgcgg
    16681 gcctggacat cgcctaccta ttgggcgggg cgatcatcac agagagcatc ttcacgctcc
    16741 agggcctcgg gcaggtgtcg atcgcgtcca tccgggactt cgatcttccg atcatcaccg
    16801 ccattacgat gatcagtgcc gcgttcatca tcgtcgccaa cctcgtcgtc gacctcctct
    16861 acgcggtcct cgatccgcgc gtcaaggtcg gctagatgat tggccggctg aacaagatgg
    16921 cgccgggcgg cgccgtggaa gtgcctgagg cggccggcga caggccgttc ctcgaggtca
    16981 aggatctatt cgtcaagttc ccgactgagg acggcctcgt ccatgcagtc gacggcgtgt
    17041 cattcacgct cgagcgagga aagacactgg cgatcgtcgg tgagtccggg tcgggcaaga
    17101 gcgtcaccgc ccaggccatc atgggcctgt tggatcgtcg tcaggccaag gtgagcggcg
    17161 agatctggct cgatcggctc aacctgctcg cgctcaagcc cgcccaggtg caggcgctgc
    17221 gcggtgagcg catggccatg atcttccagg accccatgtc gagcctgcat ccgttctacc
    17281 gggtcgggga ccagatcgtc gaggccattc aggcccaccg cagcgtcagc aagtccgccg
    17341 cgcggcagga aacgctcgac atgctcaagc gcgtcggcat ccccgcggcc gcgcggcgca
    17401 tcgacgccta tccgcaccag ctgtcgggcg gtatgcgtca gcgcgtgatg atcgccatgg
    17461 cgctcatcaa ctccccggag ctgctcatcg ccgacgagcc gaccacggcc ctcgacgtga
    17521 cggtgcaggc tcagatcctg gagctgatca ggcagctcca ggccgagttc gggacgacgg
    17581 tcatcatgat cacccacgac ctgggcatcg tcgccgacgt cgccgacgag gtcgcggtca
    17641 tgtacggcgc gcgaatcatg gagcgggcat cgacccagga gatctacgag caccccgaga
    17701 tgccgtacac gcttggcctg ttgtcctcca ttccgcgcat ggacagagat gtcaccgggc
    17761 ggctcgagcc catcaaaggc aatccaccgt cggctgtccg gctgccgtcg ggctgcgtct
    17821 ttgagccacg ctgcccgtac tcggg
//
LOCUS       DATBRX010000063         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_689890, whole genome shotgun sequence.
ACCESSION   DATBRX010000063 DATBRX010000000
VERSION     DATBRX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689890"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VIF08_02260"
     CDS             <1..420
                     /locus_tag="VIF08_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8134835.1"
                     /translation="GISLLTVEIPLVLYTLVIFVRNIAAGFDSVPRDVLEAANGLGFT
                     KAQRLWRVEVPLAVPLILAGVRLATVSTIALVTVTSILGDGFGGLGFFILEGYRRSFP
                     TELYFGAVPSVALALLADFALARVQGRLTPWHTAEAG"
     gene            421..1140
                     /locus_tag="VIF08_02265"
     CDS             421..1140
                     /locus_tag="VIF08_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8134836.1"
                     /translation="MDLLNRTIVWLSDPTHWAGPNGIPARLMEHAGLSAVSLLIALAI
                     ALPVGLWIGHTRRFVWLAAASANLWRALPSLAVIAIVLPITVAIDPQAGFKVYPTVVA
                     MVVLAIPPILVNAQAGISGVERDVVDAGRGQGMSAGQVLARIELPLAVPVIVTGIRSA
                     AIQIVATATLGAILGFGGLGRYLVEGFATFSLGGDAQVVGGAILVGALVIAVEAAFAV
                     AQYLLTPRGLRRQRSVEPVSA"
     gene            1227..2138
                     /locus_tag="VIF08_02270"
     CDS             1227..2138
                     /locus_tag="VIF08_02270"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8134837.1"
                     /translation="MRTSRRLALGAIALALLVGACSSGGGSKPTIKIGSVGFDEARVM
                     AEIYAQALEANGYTVDRAGIGLGQRPVVAPAIESGQIDLQPEYIGSRLVYEGGTAGSD
                     PAANLTALQEKLNAKNLTALNFTPAVDTNAFVVRQETATEFNLSKMSDLAAVQDQLTF
                     GLATDCLTNAVCGAPGGALSQYGLTADTIAAATLLEPCSTPMADALKNGTVHVAELCS
                     TSPEIIVNGWVSLEDDLHTQPADNVAPIVRDDYLAKLSDKDAFKKILNDVSAKIDTDT
                     LADLYKQVSVDHKDLADVARTWLQANP"
     gene            2138..>2637
                     /locus_tag="VIF08_02275"
     CDS             2138..>2637
                     /locus_tag="VIF08_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY8134838.1"
                     /translation="MDDGFLALADWRRRVAEIYAEWRRASATDPHAAWLRWRESRDEL
                     FRDHPQSPLTSDARARFGGLSYFDYDPRLRMTASYESADPTDQPHAPGAPAAGLLQLP
                     SSGAEAFSFRRIGQIRLAGPLDGASLPVFWMSGYAGGIFLPFRDATSGSATYGAGRYL
                     LDTVKGA"
BASE COUNT          404 a          903 c          872 g          458 t
ORIGIN      
        1 gggatcagcc tgctcaccgt cgagattccc cttgtcctgt acacgctcgt gatcttcgtg
       61 cgcaacatcg ccgcgggctt cgattcggtc ccgcgcgacg tcctcgaggc cgccaacggc
      121 ctcggcttca cgaaggccca gcgcctgtgg cgcgtcgagg tgccactggc tgtgccgctg
      181 atcctggccg gcgtgcgcct tgcgaccgtg tcgacgatcg cgctcgtgac cgtaacttcg
      241 atcctcggcg acggctttgg cggtctcggc ttcttcatcc tggagggcta ccggcgcagc
      301 ttcccgaccg agctctactt cggggcggtc ccgtcggtcg ccctggccct tctggccgac
      361 ttcgcgctgg cacgcgtgca gggccggctt accccctggc acacggctga ggcgggataa
      421 atggacctgc tgaaccgaac catcgtctgg ctgtcggatc cgacgcattg ggccgggccc
      481 aacggcatcc ccgcccggct catggagcat gccggcctgt cggcggtgtc gctgctgatt
      541 gcgcttgcca tcgcgttgcc cgtagggctg tggatcggcc acacgcgccg tttcgtgtgg
      601 ctcgcggcgg cgtcggccaa cctgtggcgc gccctgccgt cgttggccgt catcgccatc
      661 gtcctgccga tcaccgtggc gatcgatccg caggccggct tcaaggtcta tcccaccgtc
      721 gtggcgatgg tcgtgctggc gatcccgccc atcctcgtca acgcgcaggc cggtatcagc
      781 ggagtggagc gggacgtcgt cgacgccggc cgcggccagg gtatgagcgc agggcaggta
      841 ctggcgcgga tcgagctgcc gctggccgtg cccgtgatcg tgactggaat tcgttccgcg
      901 gccatccaga tcgtcgcgac cgcgaccctg ggcgcgatat tgggtttcgg tgggctgggc
      961 cgctatctcg tggagggctt cgcgacattc agcctgggcg gcgatgccca ggtggtcggc
     1021 ggcgcaattc tcgtcggcgc tttggtcatc gcggtcgaag cagcttttgc cgtggcgcag
     1081 taccttctca ctccgcgcgg tttgcggcgc cagcgatccg tggagccggt cagcgcgtag
     1141 atcctgagta gttcggcgcg cggttccgat gtatagtccg cgcctgacgc cggactaacc
     1201 ggatgaacga aaggagaagg ttacccatgc ggacctcccg caggctggcc ctcggggcga
     1261 tcgcgctcgc gctgctggtc ggtgcgtgct cgtcgggtgg gggcagcaag ccgaccatca
     1321 agatcggctc tgtcggcttc gacgaggcgc gagtgatggc tgaaatctat gcccaggcac
     1381 tcgaggcaaa tggctacacc gtggatcgtg ccggcatcgg cctgggccag cgtccggtcg
     1441 tggcgccggc catcgagagc ggccagatcg acctccagcc ggagtacatc ggcagccgcc
     1501 ttgtgtatga gggaggcacg gcgggcagcg atccggcggc caacctgacc gcgctgcagg
     1561 aaaagttgaa cgccaagaac ctcacggcgc tcaacttcac gcccgcagtc gataccaacg
     1621 cattcgtcgt ccgccaggag accgcgacag agttcaacct gtccaagatg agcgaccttg
     1681 ccgcggtcca ggaccagctc acgttcggtc tggcgactga ctgcctaacc aacgccgtat
     1741 gcggcgctcc cggaggcgcg ctcagccagt acggcctcac cgccgacacg atcgcagcgg
     1801 caacgttgct cgagccctgc agcacaccga tggccgacgc gctcaagaac ggcacggtcc
     1861 acgtggccga gctgtgctcg acgagccccg agataatcgt caacggctgg gtctcactcg
     1921 aagacgatct gcacacgcag ccggcggaca acgtcgcgcc catcgtgcgc gacgactacc
     1981 tcgcgaagct gagcgacaag gacgcattca agaagatcct gaacgacgtc tcggccaaga
     2041 tcgacacgga caccctggct gatctgtaca agcaggtcag cgtcgatcac aaggacctgg
     2101 ccgacgtggc ccggacctgg ctccaggcaa acccctgatg gatgacggct tcctggcgct
     2161 cgccgattgg cggcgccggg tggccgagat ctacgcggag tggcggcgcg cttctgcgac
     2221 cgacccacat gccgcgtggc tgcgctggcg cgagtcgcgt gacgagctgt tccgcgacca
     2281 cccgcaatcg ccgctgacga gtgatgcgcg agcccgcttc ggcgggctct cgtatttcga
     2341 ctacgacccc aggttgcgca tgactgcgag ctacgaatcg gccgatccga ccgatcaacc
     2401 tcacgcgccc ggcgcgccag ccgccggact gctgcagctg ccgtcgagcg gcgccgaggc
     2461 gttctcgttc cggcgcatcg ggcagatccg tctggccggg ccactcgacg gcgcgtcgct
     2521 ccccgtgttc tggatgagcg gctatgccgg cggcatcttc ctgcccttcc gcgatgcgac
     2581 ctcgggctct gccacctacg gcgcgggccg ctacctgcta gacacagtca aaggcgc
//
LOCUS       DATBRX010000064         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3261383, whole genome shotgun sequence.
ACCESSION   DATBRX010000064 DATBRX010000000
VERSION     DATBRX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3261383"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VIF08_02280"
     CDS             complement(<1..258)
                     /locus_tag="VIF08_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134839.1"
                     /translation="MDWTAWHTDYDADTPLRRRLEIVQRHIVDALASIERSPVRVISM
                     CAGEARDLRGALARVERRDLVGRLVELDPGLAGNARRGLSEL"
     gene            complement(298..963)
                     /gene="upp"
                     /locus_tag="VIF08_02285"
     CDS             complement(298..963)
                     /gene="upp"
                     /locus_tag="VIF08_02285"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY8134840.1"
                     /translation="MAADPHPDFPSLHVSSHPAVLHKLSYLRDSAVEPKKFRELVREI
                     SWLVGYEALADARVRDVKVQTPIELTDGVKLADRIGLVPILRAGLGMVDAMLELMPTA
                     QVWHLGLFRDERTLRPVEYYNKLPDSAAVDLCLILDPMLATGGSATAAIDVLKRWGAV
                     QPVRIKLLNLIAAPEGVRAVQEAHPDVPIYAAALDRQLNDKGYIMPGLGDAGDRQFGT
                     GGN"
     gene            complement(966..1163)
                     /locus_tag="VIF08_02290"
     CDS             complement(966..1163)
                     /locus_tag="VIF08_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134841.1"
                     /translation="MAGATAFVLVLSYFLLLTSGVAWGLDLVLGSIILSSVLAMGLAL
                     IVAWDRPAESDGSTESGATVS"
     gene            1366..2529
                     /locus_tag="VIF08_02295"
     CDS             1366..2529
                     /locus_tag="VIF08_02295"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8134842.1"
                     /translation="MAQTPVRPSATARKTLRQTLPPALGKTRYPRNVAAPVLVEFHRG
                     PFVESRHRGHVVQVDVTGKVQQGIGDPDMVTSVRSAIKPFALAALLDAGGAESFHLTG
                     PEIAVMASSHHGEDAHVRTIQAVLRRAGLTQALLACGTADAPEDEITAARLAREGEAP
                     GAVRHNCSGFHTASLLMSKLAGWSLADYWRPEHRSQVAMADTVARVFGVRPSALVTAV
                     DNCGVYTYAFPLVAIARAFVLLADPAGAADTARTPLIPALTRIRDAMIAAPEMVGGSH
                     NAPDTMIMRARPAQLVAKGGAEGLRGIGLLAGARGADSGAAGIAIKIEDGDWAKRANR
                     AVTIEALAQLGVLSGSTLERLADLHRPPTVDPRGVEIAQAVPSFRLAPLSELG"
     gene            2533..>2869
                     /locus_tag="VIF08_02300"
     CDS             2533..>2869
                     /locus_tag="VIF08_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8134843.1"
                     /translation="MAAAHDPYRALGVPRGASAADIKKAHRLLAKRYHPDAAKGDGNR
                     FLVVQEAYRVLSDPLLRREWDARHAPGPVRATAKVPTKRAAPNATRPARRTKAPEAET
                     TASGPAGARP"
BASE COUNT          449 a          998 c         1007 g          415 t
ORIGIN      
        1 cagttcgctc aagccgcgac gggcgttccc ggcgaggccc ggatccagct ccacgagccg
       61 cccaacaagg tcgcgtcgct cgacgcgagc gagcgcgccg cgcaggtcgc gtgcctcgcc
      121 ggcgcacatg ctgatgaccc ggacgggcga ccgctcgatg gacgcgagcg cgtcaacgat
      181 gtggcgctga acgatctcga ggcgccggcg caacggcgta tccgcgtcgt agtcggtgtg
      241 ccaggccgtc caatccaagg ggcggccgct gccctcgact gtcagctgcc caagctccta
      301 gttcccgccg gtgccgaact ggcgatcgcc cgcgtcaccc agcccgggca tgatgtagcc
      361 cttgtcgttg agctggcgat caagggccgc ggcgtagatg ggcacgtcgg ggtgcgcctc
      421 ctggacggcg cgcacgccct ccggcgcggc gatcagattc agcagcttga tgcgcaccgg
      481 ttgcaccgcg ccccagcgct tcaggacgtc gatcgccgcc gtggctgatc cgccggtcgc
      541 gagcatggga tcgaggatga ggcacaggtc gaccgcggcc gagtccggga gcttgttgta
      601 atactcgacc gggcgcaggg tgcgctcgtc gcggaacagg cccaggtgcc acacctgcgc
      661 ggtcggcatc agctcgagca ttgcgtcgac catgcccagg cccgcgcgca ggatcggcac
      721 gaggccgatg cgatccgcaa gcttgacccc gtcggtcagc tcgatgggcg tctggacctt
      781 gacgtcgcga acgcgcgcat cggccagtgc ctcatagccg acgagccacg agatctcgcg
      841 cacgagctca cgaaacttct tgggctctac tgccgagtcg cgcaggtagg aaagcttgtg
      901 caggacggcg gggtgggagg agacgtgcag gctgggaaag tcaggatgag ggtcggcggc
      961 cattctcagc tgactgtagc gcccgactcg gtcgaaccgt ccgattcagc gggccgatcc
     1021 caggcgacga tcagggccag acccatcgcc agcacgctgg acaggatgat cgagcccagg
     1081 accaggtcga ggccccaggc cacgccgctc gtgagcaaca ggaagtagct caggaccagc
     1141 acgaacgcgg tcgcgccggc catgttgagt gccaggcgcc ggatcgtcgg tcagattgcg
     1201 gggttgggtg ccgtccggat ttgccagcca gatgtcgaga ttgccgccgc ggctcgacat
     1261 gaaagcgagg gtcgatccat cgggcgacag gtgcggccac tggtcgatgc ctgggtcgtc
     1321 cgccctcacc cgggaatcgg gaggcgacgc gctaccatcc ggccgatggc ccagaccccg
     1381 gtgagaccct ccgcaaccgc gcgcaaaacg ctgcgtcaga cgctgccgcc ggccctgggc
     1441 aagacgcgct atccgcgcaa cgtagccgcc ccggtgctcg tcgagttcca ccgcgggccg
     1501 ttcgtcgaga gccgtcaccg cgggcacgtc gtgcaggtcg atgtgaccgg caaggtgcag
     1561 caggggatcg gcgatccgga catggtgacg tccgtccgat ccgcgataaa gcccttcgcg
     1621 ttggccgcgc tgctcgacgc tggcggcgcc gagtcgttcc acctgaccgg ccccgagatc
     1681 gcggtcatgg cctcttcgca ccacggcgag gatgcccatg tccggaccat ccaggccgtg
     1741 ttgcgccgcg ccgggctgac ccaagcgctg cttgcctgtg gcaccgccga tgcgccggag
     1801 gacgagatca ccgctgcccg gttggcccgc gaaggtgagg ctccgggcgc ggttcgtcac
     1861 aactgctcgg gcttccacac cgcgagcctg ctcatgtcga agctcgcggg ctggagcctc
     1921 gccgactact ggcggccgga gcaccggtca caggtcgcga tggccgacac cgtggccagg
     1981 gtctttggcg tgcggccgtc ggcgctggtc accgcggttg ataactgcgg cgtctacacc
     2041 tacgcgtttc cgctcgtcgc catcgcacgc gcgttcgttc tcctggccga tccggccgga
     2101 gcagccgaca cggcgcgcac gccgctgatc cccgcgctga ccaggatccg cgacgcgatg
     2161 atcgccgcgc cagagatggt cggcggcagc cacaacgcgc ccgacacgat gatcatgcgt
     2221 gcccgacccg cccagctcgt ggccaagggc ggcgcggaag gtctgcgcgg tatcggcctt
     2281 cttgccggcg cccgcggagc tgattcgggc gcggccggta tcgcgatcaa gatcgaggac
     2341 ggcgactggg cgaagcgggc gaaccgcgcg gtgacgatcg aggcgcttgc ccaactgggc
     2401 gtcctgtcgg gttcgaccct ggaacggctg gccgacctgc accgcccgcc gacggtcgat
     2461 ccgcgcggcg tggagatcgc gcaggcggtg ccgtcgttcc ggctcgcgcc gctgtccgag
     2521 ctgggctagc tggtggctgc tgcgcacgat ccgtaccgcg cgctcggcgt ccctcgcggg
     2581 gcgtctgccg cggacatcaa gaaggcgcat cgccttttgg ccaagcgcta ccacccggac
     2641 gcggccaagg gtgacgggaa ccgcttccta gtcgtgcagg aggcgtatcg cgtcctgtcc
     2701 gatccgttgc tgcgccgtga atgggacgca cgccacgcac ccgggccggt cagagcaacg
     2761 gccaaggtac cgactaagcg cgctgcgccc aacgccacgc ggccggcccg ccggacaaag
     2821 gcgcccgaag cggagacgac cgcgagcgga cctgccggag cgcgaccgc
//
LOCUS       DATBRX010000065         6615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2143580, whole genome shotgun sequence.
ACCESSION   DATBRX010000065 DATBRX010000000
VERSION     DATBRX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2143580"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VIF08_02305"
     CDS             <1..393
                     /locus_tag="VIF08_02305"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF027523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8134844.1"
                     /translation="EVACSAGTYIRAIARDLGDQLGSGAYLAALTRTASGPFTIDAAH
                     ALDEVRAALANGRADGMLLAMDTGLTFPEVTLSADELVPFARGQQLRRAGTLPGEGLV
                     RVRDGSGRLVAIARVADGVLAPEKVFAA"
     gene            390..1346
                     /locus_tag="VIF08_02310"
     CDS             390..1346
                     /locus_tag="VIF08_02310"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY8134845.1"
                     /translation="MTLTLRSIDDLPPRLRFALAIGVFDGAHRGHKRVVRALLAAAQR
                     HHAEPVALTFDPHPSEIVRGAAPPLLCDPDERVALLHGLGVTTVVLQHFDKPFAEQSP
                     EDFLERVRSGRELVALVMTPESAFGHDREGTIAKIRRLAPALGFELVEVGQLASSGAV
                     VSSTRVRDALAAGRLADVRRLLGRPYTVTGHVVRGDRRGRALGFPTANLSFQSPVALP
                     ADGIYAVRAAWGGRDRLNPARRADGVASLGVRPTFGPGGARVLEVHLFDIDENLYGKR
                     MRVELVRRLRGEKRFASAAALVRQMDRDAARARAVLTASRGR"
     gene            1404..1673
                     /gene="rpsO"
                     /locus_tag="VIF08_02315"
     CDS             1404..1673
                     /gene="rpsO"
                     /locus_tag="VIF08_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY8134846.1"
                     /translation="MPLARETKEGIVADYARADADTGSPEVQIALLTERIKGLTEHLR
                     AFPKDNHSRRGLLKLVGQRRRLLAYLMRKDNARYRAVIGSLGLRR"
     gene            1705..4293
                     /gene="pnp"
                     /locus_tag="VIF08_02320"
     CDS             1705..4293
                     /gene="pnp"
                     /locus_tag="VIF08_02320"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8134847.1"
                     /translation="MPVTTLTAEVGGRTLTIETGKLALLAGGSVTVRYGDTMLLGTAN
                     RSEPRPGLDFFPLTVEFEERMYAAGKIPGGFIKRESRPSEAAILAARLTDRPIRPLFP
                     EGYKDDVQVVLTVLSTDQQNDPDIIGTIAASAALTISEIPFKGPIAAVRVGRINGELV
                     LNPTVSQLDESDIDLIVSGTRDAIMMVEAGAKIVPEAVMAEAIMFGHRSLAPILELQE
                     KLREQVGKPKRVPYIEPGTESVLDFVDAFKANKPFVVFDIETTSRDVKQGSMVEIGAV
                     KVAGGKITDKWSTLVNPGMTIVGRQLHGLTDADVKDAPTPAEAAKTFLDWAGDALLVG
                     HNVGFDIGFLEAASGQHFEAGRYVDTLVLAREAYPDGDLKLGDLARFFALEVEPNHRA
                     LPDAEATAELLIRLGNDLPARIDAFKKTVADTIRASKGMGPDKATTYGMSDEARKTAR
                     FSKSLAGVLHKKTVRELVLNEGLRMDGRDLDTIRPISVEVGLLPRAHGSGLFTRGQTQ
                     ALSVATLGPSSDVQRIDTISPEESKRYLHHYNMPPYSTGENKPMRGPGRREIGHGALA
                     ERALLPVLPSSEEFPYVIRVVSECVTSNGSTSMASTCGSTLALMDAGVPIKAPVAGAA
                     MGLVTGENGKFAVLTDILGKEDAFGDMDFKVTGTREGITALQMDIKVTGINEAIIRDG
                     LEKALVARLAILDKMLEVMPTTRSEMSAYAPRIITIKINPEKIRDIIGKGGATIRKIQ
                     EDTGTEINVEDDGTVEIAAIDADKSRAAIQMIEGLTREVEVGGLYLGKVTRIMTFGCF
                     VEILPGKEGLVRIGELADYHVPTVEDVVSVGDEIMVVVVEIDRQGRVNLSRKAAMQRH
                     LAKTPA"
     gene            complement(4479..5135)
                     /locus_tag="VIF08_02325"
     CDS             complement(4479..5135)
                     /locus_tag="VIF08_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY8134848.1"
                     /translation="MSNRSRGRPRHPDVLTPTEWSVLDMYRHGMPRRAIAWRRGTSEY
                     AVRYHLRNATSKLGLARTQDLRHWQGSPATSPVNASEHRSRRFKTMDSEIQLGELAQV
                     SMLCRSATATETWYRDVLRLPHIFTFGALVFFDMNGTRLYFREVPDKEFRASSTLYFA
                     VDDINVATDLLKGRGVKFQGAPHMIYKDDATRVEEWFSFFEDPDGNTLALLARVPSST
                     "
     gene            5306..6103
                     /locus_tag="VIF08_02330"
     CDS             5306..6103
                     /locus_tag="VIF08_02330"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8134849.1"
                     /translation="MNRDERKRALDQIAAEVSVCRLCPLHVGRTNAVPGEGHPDTEVV
                     FVGEGPGFNEDQQGRPFVGAAGGLLNELLKAIGWKREEVFITNVVKCRPPQNRDPEPS
                     EIAACAPYLRRQLEILDPAVVVTLGRHSLQTFMPGARIGGVHGTVHPVDSQTGAANAQ
                     TYAMYHPAAALRASSLKQTMLEDMSGLPQVLIDARAKRAGGIASPLQSVAATAVVTEP
                     VTLEPVAAEPVAVDMELQVEAEQVLAAVAVTADTVPEPVDENQMSLF"
     gene            6104..>6615
                     /locus_tag="VIF08_02335"
     CDS             6104..>6615
                     /locus_tag="VIF08_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY8134850.1"
                     /translation="MLNDQSRVLPAAPGDVLRVLPLGGVGEIGKNMYVFEYGDEIVVI
                     DCGLMFPEEEMFGIDLVVPDVTYLRQRREKVKAFLITHAHEDHVGGLPYVLPDFPGVP
                     IYASTLARGLLGGKIKEHKLNNNPLLALEPGETVELGAFSATAFRIGHSIPDAMGIAL
                     RTPVGLVVHTG"
BASE COUNT         1125 a         2231 c         2185 g         1074 t
ORIGIN      
        1 gaggtcgcct gctcggccgg gacgtacatc cgcgccatcg cgcgcgacct gggcgatcag
       61 ctgggcagcg gggcgtacct ggcggcgttg acacgtacgg ccagcggccc gttcaccatc
      121 gacgccgcac atgcgctcga cgaggtccgc gcggcgctcg ccaatggacg cgcggatggc
      181 atgctgctgg caatggacac agggctcaca ttcccggagg tcacgctgtc agccgatgag
      241 ctggtgcctt tcgcgcgtgg ccagcagctg cgccgcgcgg gcacgctgcc gggggagggc
      301 ctcgtccgcg tgcgcgacgg cagcggtcgc ctcgtggcga tcgcgcgggt cgcggatggc
      361 gtgctcgccc cggagaaagt gttcgccgcg tgacgctgac cctgcgctcg atcgacgatc
      421 tgcccccgag gctgcggttc gcactcgcga ttggcgtgtt tgatggcgcc caccgcggcc
      481 acaagcgcgt cgtccgcgca ctgctcgcag ctgcgcagcg ccatcacgcg gagccggtgg
      541 cactgacgtt cgacccgcac ccgtcggaga tcgtgcgcgg cgcagcgccg ccactgctgt
      601 gcgaccccga cgagcgtgtc gccttgctcc acggtctggg tgtcacgacc gtggtgctgc
      661 agcacttcga caagccgttt gccgagcagt cgcccgagga cttcctggag cgcgtgcgga
      721 gcggccgaga gctggtcgcg ctggtcatga ccccggaatc ggcattcggc cacgaccgcg
      781 aggggacaat cgccaagatt cggcgcctgg cgccggctct ggggttcgag ctcgtggagg
      841 tcggccagct ggccagtagc ggtgcggtcg tctcgagcac cagggtgcgt gacgcgctgg
      901 ctgctggtcg cctggcggac gtgaggcggc tcctgggccg gccctacacg gttacaggcc
      961 acgtcgtgcg cggcgaccgt cgcgggcgcg ccctgggctt tcccacggcc aacctgtcat
     1021 tccaatcgcc cgtcgccctg cccgctgacg gcatctacgc cgtgcgcgca gcatggggcg
     1081 gacgcgaccg cctcaatccc gcgcgccggg cagatggtgt ggcgtcactc ggcgtgcggc
     1141 caacgttcgg ccccggcggc gcgcgcgtcc tggaggttca cctcttcgac atcgacgaga
     1201 acctctacgg caaacgaatg cgcgtcgagt tggtgcgccg gctgcgcggc gaaaaaaggt
     1261 tcgcgtcagc cgccgccctg gtccgccaga tggaccgtga cgccgccagg gcgcgcgccg
     1321 tcctgactgc atctcggggg cgctaaagcc gggtgactgc tagactccga gcgataagag
     1381 ataaggaaac attggaggaa cgggtgcccc tggcgcggga aaccaaggaa ggcatcgtgg
     1441 cggactacgc ccgagcagac gccgatacag gctcaccgga ggtccagatc gcgctcctga
     1501 ctgagcggat caagggcctc acggagcatt tgcgtgcctt tcccaaggac aatcactcgc
     1561 gccgcggctt gctcaagctc gtcgggcagc gacgccggct gcttgcgtat ctgatgcgca
     1621 aggacaacgc tcgctatcgc gccgtaatcg gcagcctcgg actgcgccgc taagcaagac
     1681 ccgtcctccc cacttggagg actgatgcca gtcactactc tgaccgccga agtcggcggt
     1741 cgaaccctga ccattgagac gggcaagctc gccctgctcg ccggtggttc cgtcaccgtg
     1801 cgctacggcg acacgatgct gctcggcacc gccaaccgct ccgaaccgcg tcccgggttg
     1861 gatttcttcc cgttgaccgt cgagttcgag gagcgcatgt acgccgcggg caagatcccg
     1921 ggcggcttca tcaagcgcga gtcgcgcccg tcggaggcgg ccatcctggc tgcgcgtctg
     1981 accgaccggc ccattcgtcc gcttttcccc gagggctaca aggacgacgt ccaggttgtc
     2041 ctgaccgtcc tgtcgaccga ccagcagaat gatcccgaca tcatcggcac gattgccgcc
     2101 tcggccgcgc tgacgatcag cgagattcct ttcaagggcc cgatcgcagc ggtccgcgtc
     2161 ggccgcatca acggtgagct ggtgctcaac ccaaccgtca gccagctcga cgagtcggac
     2221 atcgacctga tcgtgtccgg cacccgtgac gcgatcatga tggtcgaggc cggcgccaag
     2281 atcgtgcccg aagccgtcat ggccgaggcg atcatgttcg gccaccgctc actggcgccc
     2341 atcctggagc tccaggagaa gctgcgcgag caggtcggca agcccaagcg cgtgccgtac
     2401 atcgagccgg gcacggagtc tgtgctcgac tttgttgacg ccttcaaggc caacaagccc
     2461 ttcgtcgtct tcgacatcga gaccaccagc cgcgacgtga agcagggctc catggtcgag
     2521 atcggtgccg tcaaggtggc cggtggcaag atcaccgaca agtggtcgac cctggtcaac
     2581 ccgggcatga cgatcgtcgg tcgccagctg catggcctca ctgatgcgga cgtcaaggac
     2641 gcgccgacgc cggccgaggc tgccaagacc ttccttgact gggcgggcga cgcactgctc
     2701 gtcggtcaca acgtcggctt cgacatcggc tttctcgagg cggcctcggg ccagcacttc
     2761 gaggccggcc gctacgtcga cacgctggtc ctcgctcgcg aggcctatcc ggacggcgac
     2821 ctgaagctgg gcgacctggc gcggttcttc gcgctcgagg tcgagcccaa tcatcgtgct
     2881 ctgcccgacg ctgaggccac ggccgagctg ctgatccgcc tgggcaatga cctgcccgcg
     2941 cgtatcgacg ccttcaaaaa gacggtcgcc gacaccatcc gcgcgtcgaa gggcatgggc
     3001 ccggacaagg caaccacgta cggcatgagc gacgaggcgc gcaagacggc gcgtttctcg
     3061 aagagcctcg ccggcgtgct gcacaagaag acggtccgcg agctcgtcct caacgagggc
     3121 ctgcgcatgg acggccgtga tctcgacacg atccgtccga tcagcgtcga ggttggcctc
     3181 ctgccgcgcg cccatggctc cgggctcttc acccgcggcc agacccaggc gctgagcgtc
     3241 gccacgcttg gtccgtcgtc ggacgtccag cgtatcgaca ccatcagccc ggaggagtcg
     3301 aagcgctacc tccaccacta caacatgccg ccctattcga cgggcgagaa caagccgatg
     3361 cgcggccccg gccggcgcga gatcggacat ggcgcactcg ccgagcgcgc cctgctcccg
     3421 gtcctgccga gctccgagga gttcccgtac gtcattcgcg tcgtatccga gtgcgtcacg
     3481 tcgaacggtt cgacgtcgat ggcctcgacc tgcggctcga ccctcgcgct catggatgcc
     3541 ggcgtgccca tcaaggcacc cgtcgccggc gccgcgatgg gcctggtcac aggcgagaac
     3601 ggcaagttcg ccgtcctgac cgacatcctg ggcaaggaag acgcgttcgg tgacatggac
     3661 ttcaaggtca ccggcacccg cgagggcatc accgcgctcc agatggacat caaggtcacg
     3721 ggcatcaacg aggcgatcat ccgcgatggt ctcgagaagg ccctcgtcgc tcggctggcg
     3781 atcctcgaca agatgctcga ggtcatgccc acgacgcgca gtgagatgag cgcctatgcg
     3841 ccgcgcatca tcacgatcaa gatcaacccg gagaagatcc gcgacatcat cggcaagggt
     3901 ggtgccacga tccgcaagat ccaggaggac accgggacag agatcaacgt cgaggacgac
     3961 ggcacggtcg agatcgcagc gatcgacgcc gacaagtcac gcgccgccat ccagatgatc
     4021 gagggtctga cccgcgaggt ggaggtcggt ggcctttatc tcggcaaggt gacccggatc
     4081 atgaccttcg gctgcttcgt cgagatcctg ccgggcaagg aaggcctggt gcgcatcggc
     4141 gagctggccg actatcacgt cccgaccgta gaggacgtcg tctcagtggg cgacgagatc
     4201 atggtcgtgg tcgtggagat cgaccgccag ggtcgcgtca acctgtcgcg caaggcggca
     4261 atgcagcgcc acctggccaa gacgcccgct taagcggcgc aaccgcagag caatcaggaa
     4321 cggcccgctt agaggcgggc cgtttctctt gttcggcgcc ttttcaacag cgcgaacgcc
     4381 tcgttgaggc cgcgacacca ccatcgtcac gaacgcggct actagcggtc agcggtgcac
     4441 ttgacgatag tggtgtcaca aacgcagcgc gcccccacct aggtcgatga cgggacgcgc
     4501 gccagcagtg cgagcgtatt gccatcggga tcctcgaaga acgagaacca ctcctccacg
     4561 cgggtcgcgt cgtccttgta gatcatgtgc ggcgcgccct ggaacttgac gccgcggccc
     4621 ttcaggaggt ccgtggcgac gttgatgtcg tcgaccgcga agtagagcgt cgagctggcg
     4681 cggaactcct tgtccgggac ttcacggaag tagagccgcg tgccgttcat gtcgaagaac
     4741 accaacgcgc cgaacgtgaa gatgtgcggc agccgcagga catcgcggta ccaggtctct
     4801 gtcgcggtag cagagcggca cagcattgac acctgtgcga gctcgccgag ctggatctct
     4861 gaatccatcg tcttgaacct cctcgagcgg tgttcgctcg cattgactgg acttgtcgcc
     4921 ggcgagccct gccaatgacg caggtcctgc gtcctggcga gcccgagctt tgatgtcgcg
     4981 ttgcgcaagt ggtacctgac tgcgtactcg ctcgtgccgc gccgccatgc gatcgcgcgg
     5041 cgcggcatgc cgtggcggta catatcgagc accgaccact cggtcggcgt gagaacgtcc
     5101 gggtgacgcg gtcggccgcg cgatcggttg ctcatcggcc atagcctatc gggctaccac
     5161 ggattttccc tcccccgatg accgggcccg gagttcttcc gctcaggagc cacgggaagg
     5221 gcaggggcga tgtaccatcg aggccgtgcc catcggtatc cgctttgagt gaatccatgt
     5281 ccatcaccga gcaccaaggg cacacgtgaa ccgcgacgag cgcaagcgcg cactggatca
     5341 gatcgcggcg gaagtctcgg tctgccggct ctgtccgctc cacgtgggtc gcaccaacgc
     5401 ggtcccgggg gagggccatc cggacaccga ggtcgtcttc gtcggcgaag gccccgggtt
     5461 caacgaggac cagcaggggc ggccgttcgt cggcgcggcc ggtggactgt tgaacgaact
     5521 gctgaaggca atcggctgga agcgcgagga ggtcttcatc accaacgtcg tcaagtgccg
     5581 gccgccgcag aatcgcgacc cggaaccatc ggagatcgct gcttgcgccc catacctgcg
     5641 ccgccagctg gagattctcg acccggccgt ggtagtcact ttggggcggc attcgctcca
     5701 gacgttcatg cccggggcgc gcatcggcgg cgtgcacggc accgtgcatc cagttgactc
     5761 tcaaaccggc gccgcgaacg cccagactta cgcgatgtat cacccggccg cggcgctgcg
     5821 cgcgagcagt ctgaagcaga cgatgcttga ggacatgagc ggcctacccc aggtgcttat
     5881 cgatgcgcgc gcgaagcgtg ccggcgggat cgcttcgcca ttgcagagcg tcgccgcgac
     5941 agccgtggtg acggagcccg tcacgctcga gcccgtcgcg gccgagcctg tcgcggtcga
     6001 catggagctc caggtcgaag ccgagcaggt cctcgctgcc gttgcggtga ctgccgatac
     6061 cgtgccagag ccggtcgacg agaaccagat gagtctcttc tagatgctga acgaccaatc
     6121 acgcgtcctg cccgcggcgc ccggtgacgt gctgcgcgtc ctgcccctgg gcggcgtggg
     6181 cgagatcggc aagaacatgt acgtgttcga gtacggcgac gagatcgtcg tgatcgactg
     6241 cgggctcatg ttccctgagg aggagatgtt cggcatcgac ctggtcgtgc ccgacgtcac
     6301 ctacctgcgc cagcgccgcg agaaggtgaa ggcgttcctc atcacccacg cccacgagga
     6361 ccacgtgggc ggcctgccgt atgtgctgcc ggattttccc ggcgtgccca tctacgcctc
     6421 gactcttgcc cgcgggctac tgggcggcaa gatcaaggag cacaagctca acaacaatcc
     6481 gctcctcgcc ctcgaacctg gtgagacggt cgagctgggc gcgttcagtg cgacggcctt
     6541 ccgcattggc cactcgatcc ccgacgcgat gggcattgcc ctgcgcacgc cggtcgggct
     6601 ggtcgtgcac acggg
//
LOCUS       DATBRX010000066         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1697110, whole genome shotgun sequence.
ACCESSION   DATBRX010000066 DATBRX010000000
VERSION     DATBRX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697110"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..676
                     /locus_tag="VIF08_02340"
     CDS             164..676
                     /locus_tag="VIF08_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8134851.1"
                     /translation="MTILFPPPISPSLVIGLAALILGVLGFATAARLRGSTLGSMVVT
                     LRLLFLAVGVVMGLSGFFAPTPQTRLANPIPATVDSISAGEGVYLNTCAACHGVDARG
                     GGPQSGTTRVRPPALVGPGSHLGQHTDGDLHYFIGNGLPGGMPAWANRLTDDEIWNVI
                     NFLRDLHSAP"
     gene            857..1894
                     /locus_tag="VIF08_02345"
     CDS             857..1894
                     /locus_tag="VIF08_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8134852.1"
                     /translation="MDTVTLPAVALPPSIFVAEDDLATSALLTATLERANYEVRHADD
                     GADALTQILRVPPDVALLDIGMPGLDGLQVTRALRRSPTTALLPIILVTARGRLEDKV
                     AGLDAGASDFITKPFEPAELLARVRSNLRLSAALARLESAQDVLVALASAVDAKDAQT
                     EHHCSRVAEAALKLAKHHGLSGEQLEAVGYGAVLHDVGKIGIADSVLTKPGLLTPEER
                     LEMQKHPLVGAAILRPLRIGRLVEPIVRGHHERFDGTGYPDRLHGEAITLGARIVTVV
                     DSYDAMTHSRPYRAAMSLDQARDELLRHRGSQFDPALVDLFLESIDLPPAPDPELDAV
                     TRGLRYIRGDA"
     gene            1891..>2653
                     /locus_tag="VIF08_02350"
     CDS             1891..>2653
                     /locus_tag="VIF08_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134853.1"
                     /translation="MSGLTFAVEIAFGLLFVATFVTWLRRRDAVSLDVMLIFAALAGL
                     FFVSAISTVSTNQELAGALALALLFAQPVLTLRLVARIRPIRRSVLVGAGLVWALTAV
                     PPIFISMRVVWPLLFGGLIAFVITDIVAAVYLWLEARKRVASAKARLVAAAAGSALMA
                     AAILVSIIGPALTHSSGSASEAGRATALMAALLFAGAFVAPQWIRRIGGAITSAQFTG
                     HLLSGPAGERPEEIWSRLAELARRTTGASVGLVAIG"
BASE COUNT          375 a          934 c          870 g          474 t
ORIGIN      
        1 gcagcggcgg tctttggctt caccatcgtc ctcgccggcg gggcactgcc ccgggtcgaa
       61 cgacgcctgg gccgcctgac gctcttggct ggatccatag ccgccgcgct cctggcggtc
      121 gcggcattta tcgtgggacc acaactgtga ggtacgtctt ccagtgacga tcctcttccc
      181 gccgccgatc agcccgagcc tggtcatcgg gctggctgcc ctgatcttgg gggtcctggg
      241 cttcgccacc gccgcccgcc tgcgcggctc gactctcggc agcatggtgg tgacgctgcg
      301 cctgctgttc ctggccgtgg gtgtggtgat gggcctgtcg ggtttcttcg cgcccacacc
      361 gcagactcgg ctggccaacc cgattccagc cacggtcgac tcaatcagcg caggcgaggg
      421 cgtctacctg aacacctgcg ccgcgtgcca cggggttgat gctcggggtg gtggaccgca
      481 gtcgggtacg acacgggtcc gaccaccggc gctggtcggt ccgggcagtc acctgggcca
      541 acacacagat ggcgacctgc actacttcat cggcaacggc ctgcccggcg gcatgccggc
      601 ctgggccaat cgccttaccg atgacgagat ctggaacgtc atcaatttcc tgcgcgacct
      661 gcactcagcg ccctagggcc gggagaggcg ctgctcgacc accggagcgc ggcgtatacg
      721 cctggatccg gcgcaggaac cgcgcgtttg gtacaccggt cgggctactg cgcggttcca
      781 gacggcggcg aaggtaccgg tgtcctgacg cacctgttgc tgccactctg tggggaacca
      841 gcaccgaggc ctaggattgg acacggtcac gctcccagcg gttgccctgc cgccatcgat
      901 cttcgttgcc gaagacgacc tcgcgacaag cgccctgttg acggccacgc tcgagcgggc
      961 caattacgaa gtgcgccacg cggatgacgg cgccgatgcg ctgacccaga tcctgcgtgt
     1021 gcccccggac gtcgcgctgc tggacatcgg catgcccggc ctcgacgggc ttcaggtcac
     1081 gcgcgcgctg cggcggtcgc ccacgaccgc gctcctgccg atcatcctcg taacggcgcg
     1141 cggccgcctc gaagacaagg tcgccggcct ggatgccggc gcgagcgact tcatcaccaa
     1201 gccgttcgag ccggccgagc tcctggcacg ggtccgatcg aacctacgcc tttctgccgc
     1261 cttggcccgt ctcgagagcg cccaggatgt gctcgtcgcg ctggccagcg cggtcgacgc
     1321 caaggacgcg caaaccgaac atcactgcag ccgcgtggct gaggctgcgc tcaagctggc
     1381 caagcaccat ggcttgagcg gcgagcagct ggaggcagtg ggctatggcg cggtgctgca
     1441 cgatgtgggc aagataggca tcgccgatag cgtgctgacc aagcccgggc tactgacacc
     1501 tgaagagcgc ctggagatgc aaaagcaccc gctcgtgggt gcggcgatcc tgcggccgct
     1561 gcgcattggc cgcctggtcg agcccattgt gcgcggtcac cacgagcgct tcgacggcac
     1621 cggctaccca gaccgtctgc atggcgaggc cattacgctg ggagcgcgca ttgtgaccgt
     1681 cgtcgactcg tacgacgcca tgacgcatag ccgtccgtat cgcgcggcga tgagcctcga
     1741 ccaggcgcgc gacgagctgc tgcgccacag gggcagccag ttcgaccccg cgctcgtgga
     1801 tctgttcctt gagtcgatcg acttgccgcc cgcgccagac cccgagctcg acgcggtgac
     1861 gcgcggcctg cgctacatca ggggtgacgc atgagcgggc tgacgttcgc ggttgagatc
     1921 gcgttcggcc tcctctttgt tgcgacgttc gttacctggc tgcgccggcg ggacgccgtc
     1981 agtctcgacg tgatgctcat ctttgccgcg ctggccggcc tcttcttcgt atcggccatc
     2041 agcacggtga gcaccaacca ggaactcgcc ggcgccttgg cgctcgcgct cctctttgcc
     2101 cagcccgtcc tcacgctcag gctcgtcgcc cgcattcgcc ccattcgccg cagcgtcctc
     2161 gtcggcgcgg gtttggtatg ggcccttact gccgtgccgc ccatcttcat cagcatgcgc
     2221 gtggtgtggc cgcttctgtt cggcggcctc attgccttcg tcatcacgga catcgtggcc
     2281 gcggtctacc tgtggctcga ggccaggaaa cgcgtcgcat ctgccaaggc ccggctggtc
     2341 gcagccgctg ccggtagcgc gttgatggcc gcagcgattc ttgtttcgat aatcgggccg
     2401 gcgctcaccc actcgagcgg ttccgcctcg gaggccggcc gcgccaccgc ccttatggct
     2461 gccctcctgt tcgccggtgc gttcgtcgcg ccgcaatgga tacgccgcat tggcggggcc
     2521 atcacttctg cccaattcac aggtcatcta ctgagcgggc ccgccggcga gcggccggag
     2581 gagatctggt cacggctggc cgagctggcc cggcgcacca ccggcgcgtc agtcgggctc
     2641 gtcgccattg gca
//
LOCUS       DATBRX010000067         9968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2590305, whole genome shotgun sequence.
ACCESSION   DATBRX010000067 DATBRX010000000
VERSION     DATBRX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590305"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VIF08_02355"
     CDS             <1..1212
                     /locus_tag="VIF08_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134854.1"
                     /translation="FRDQYPGGLTDAVVFLNGCATFGDGATDLGNAIRGSTSVVLGWS
                     RSVISQDAQAAALAMYDGLAGNGRTVANALEQLGDLSSSEWQNVAGQTVVSTLSSSGR
                     AAGGDLRIRDIVDFENSAGASLADGASVPLIGEPGDGQADAVTWQVRVEGIEPQAASA
                     LLRVTIDGHAAPPVAISTGTTQADDAWQLSGTLDLGVDVSVPHPSQFDASIELPEGGE
                     SVDVVSATLVAPEPTPGTSPFGSEWRGHVSQRTEVPHDGVWTVAEADLVFTLVPHTVP
                     SPYYVYELTGGTMFFSVSGADSEGCTFELSPVEIPITPEMAESAQGFTIDASGATPTF
                     RGFVHVVGPVVEVMQTCPAPYEYLTGPYSTSATGIFIDVSSDEGRAVIGDRISGTSLD
                     GSKTFDISRAD"
     gene            complement(1231..2757)
                     /gene="gltA"
                     /locus_tag="VIF08_02360"
     CDS             complement(1231..2757)
                     /gene="gltA"
                     /locus_tag="VIF08_02360"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEY8134855.1"
                     /translation="MNDETTRPVGEPLVPIEDRLDHPLGERAAVTPKERMTILRQLMP
                     EQDADARAHNFAEVNLGLPERVAMLEAERCLQCKNPVCITGCPVRVNIPRFIDLLARG
                     DMRAAADSLLDDNALPCVTGRVCPQESQCEAVCLRGKKGDAVAIGYLERFVADWANKH
                     RESAPVAPASSGKSVAIVGSGPAGLTAAGELAKRGHAVTVFEAFHTAGGVLIYGIPEF
                     RLPKDIVHDEVTRLEELGVKIEPNAIVGKTYTLDELRQTFDAIFIAVGAGLPVFMDIP
                     GENMKGVYSANEYLTRVNLMGAYRADADTPVLHGQRVAVVGGGNVAMDAVRTARRLGA
                     AEATIVYRRGMEELPARREEVHHAQAEGIKFETQVAPVEVVGENGWVTGLKCVRMELG
                     EPDESGRRRPSPVAGSEFVIGCDMVVVAIGTRSNPILTTSAAGLKVNEWGYIEVDGAG
                     MTSLPGVFAGGDIVRGAATVILAMGDGKRAAAAIDAYVTGALPAQAETTLPEAAAIAG
                     "
     gene            complement(2754..3635)
                     /locus_tag="VIF08_02365"
     CDS             complement(2754..3635)
                     /locus_tag="VIF08_02365"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HEY8134856.1"
                     /translation="MHPITGKSQLSPNVTRLVLEAPRIAQVRQAGQFVIVRAAEGAER
                     IPLTIADADPEAGTITLIIQAIGKSTEDLVALEVGQAIRDVVGPLGRETELLDHGRAV
                     CVGGGVGTAVMLPLAQGLARNGVGVTSIIGGRSREWVILEEELKRYGEVITCTDDGSY
                     GRPGFVTQALADVIADGPIDVVYAVGPVPMMRAVAEMTRPTGIQTVVSLNPIMIDGTG
                     MCGGCRVSVGGKTLYACVDGPEFDAHQVDFALLSDRLSPYRDFEKEALEKRHCQLGPQ
                     IAEVNAAAVAAPAGTAR"
     gene            complement(3777..4616)
                     /locus_tag="VIF08_02370"
     CDS             complement(3777..4616)
                     /locus_tag="VIF08_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8134857.1"
                     /translation="MTHHDSDPRVIALEHEQYNARLIARVDHTPELATFWVRFEGDPV
                     HFDPGQYMTIGVYTDDKIVQRPYSVASPPEVAGSEGYEFYVRLVPILRFTTLLWRLPI
                     DHPMRMIGPKGRFLLEPNDERTHLFVSTGTGIAPFISMMRQGLIHRQPRRVVMVNGSS
                     YVDELGYRDVLESWERDKTYPVTYVPTISRPSDPRNAGWEGRTGRVEGVVLDVCRDLH
                     LRPEKTVVYICGNPDMILNVEGLLMNAGFPEFHVKKELYWPKGKTAEVAPAAPATTAT
                     SAS"
     gene            complement(4639..4998)
                     /locus_tag="VIF08_02375"
     CDS             complement(4639..4998)
                     /locus_tag="VIF08_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134858.1"
                     /translation="MYERYDQPMLSRRAFVARVLRHAGLAGLLLLGSLAVGVAGYMFL
                     AHLSFVDAFLNAAMILGGMGPVDVLITDGAKLFAGVYALYCGLFFLVTAGVILAPFLH
                     RVLHSLHLDDDDDDQPR"
     gene            complement(5078..5608)
                     /locus_tag="VIF08_02380"
     CDS             complement(5078..5608)
                     /locus_tag="VIF08_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134859.1"
                     /translation="MSAPIAAPTRRGQSRTALIVGFVLVLVGGGALASQWLPDLDRYV
                     PLVIGLGLLAVFLMTRSYVALVFAGILTGVGAGLLVADLLPGTKDDGPGAVLGLAFGF
                     LGIWGVSWLFRLKEHHFWPLIPGGILSLVGIGLVLDLFDTDASKWVVPAIVVAIGVLV
                     MAYGYMRMAGGATTGD"
     gene            5787..8582
                     /gene="ppdK"
                     /locus_tag="VIF08_02385"
     CDS             5787..8582
                     /gene="ppdK"
                     /locus_tag="VIF08_02385"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY8134860.1"
                     /translation="MTQSPTLPAERAHRAAKRYIYAFGGGRAEGDAKMRDQLGGKGAG
                     LAEMTNAGLPVPPGFTITTEACNDYFAAGEKLPDGLWDDVLAAVAQVERETGKGLGDP
                     ANPLLVSVRSGAKFSMPGMMDTVLNLGLNESTLNGLVALTGNERFGWDAYRRFIQMFG
                     RIVMDVDGTKFEHAIDAAKEARGVKQDTELDADALRELVGTFLRVVKESTGREFPTDP
                     HEQLDLAIKAVFASWFGKRAHDYREYNHIAHDLGTAVNVVTMVFGNMGDDSGTGVAFT
                     RDPNTGEKVLFGEYLTNAQGEDVVAGIRTPQPISHMKDELPEAYAEFERIGQRLEKHY
                     KDVQDLEFTIERGRLYMLQTRSAKRTAAAAVRIAADLVEEGIITEREALSRIEPAHVD
                     QLLRDQFDPAAKTGAKKITAGLNASPGAAVGKAVFDADRAAEMAGQGEKVILVRIETS
                     PDDFHGMAAAQGVLTARGGATSHAAVVARQIGKPCVAGCAALVIDYGTKSARVNGLGF
                     SEGEWISLDGTTGEVFVGALPTTAARFEDQAELQQVLAWADKVRRLEVWTNADKPEEA
                     AQARRYGAQGIGLCRTEHMFREGERLDIVRDAILVAFQATRAKEKAAAGTILSTDDAA
                     AVASFDEAIAKLEKLQEGDFYGIFEAMDGLPVVIRLIDPPLHEFLPNHSEVLVEVTRM
                     EAKGVPADDADYVKAKELLRAVEALKEQNPMLGLRGIRLGLMIPDLIKMQTRAILNAA
                     IRRQNAGGHPHAEIMIPLVGHVNELAATRKILDAEAKAVQEAAGTTVDFMFGTMIEIP
                     RGALTADQIAEHAEFFSFGTNDLTQMAFGYSRDDAEGGFLLNYVERGILPDNPFQTLD
                     TVGVGQLVRIAVERGRAARPGLKIGICGEHGGDPASIAFFNEVGLDYVSCSPFRVPVA
                     RLAAAQAALSGAAERDK"
     gene            8582..9379
                     /locus_tag="VIF08_02390"
     CDS             8582..9379
                     /locus_tag="VIF08_02390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8134861.1"
                     /translation="MNEPPVVWHYGLMADHWAEFNTATPELEFIREAVSKYGEPVLDL
                     ACGAGRLLVPLLSAGVDVDGADISSDMIERCRQAAARVGRSTNLYCQQMDQIDLPRRY
                     RTIYLCGSFGLAGSRQRDLETLRRCRASLADGGALIFDIDAEYASPESWNKWLPEHAS
                     RLPEAWPKSGSRQTDADGAEHRAFFRAIEDDPLELTYTREVRLERWEKGSLVASEQYS
                     LRGNAYLKPEVELMLRVAGFGDVDVRGDWREQPASVTSEQVVFIART"
     gene            complement(9471..9806)
                     /locus_tag="VIF08_02395"
     CDS             complement(9471..9806)
                     /locus_tag="VIF08_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY8134862.1"
                     /translation="MQADRTLTCADCGQEFVFTASEQQFYQERGFSDPRRCRSCRAAR
                     KASMGSGDSMGGGGGYSAGGGGGFRERRPRELFDATCSNCGKTAQVPFRPTSGKPVFC
                     DDCFQRRRA"
BASE COUNT         1704 a         3314 c         3370 g         1580 t
ORIGIN      
        1 ttccgcgacc aatacccagg cgggctcacg gatgcggtcg tgtttctcaa cggctgcgcc
       61 acgttcggcg atggtgcgac cgatcttggc aatgccatcc gcggctcgac ctccgtcgtg
      121 ctgggctgga gccgttcggt catcagtcag gatgcgcagg cggcggcctt ggccatgtac
      181 gacgggcttg caggcaacgg acgcacagtg gccaacgccc tcgagcagtt gggtgatctg
      241 tcgagcagcg aatggcagaa cgtcgccgga cagaccgtgg tttcgaccct gagctccagt
      301 ggccgcgccg ccgggggcga tctgcgcatc cgtgacatcg tcgatttcga aaacagcgca
      361 ggtgcgtcgc ttgcggacgg tgcctccgtg ccgctcatcg gcgagccggg tgacggtcag
      421 gcagatgccg tcacgtggca ggtgcgcgtc gaagggatcg agccgcaggc agcctcagcg
      481 ctgctgcgcg tgacgattga cggccacgct gcgcccccgg tcgcgatctc aactggaacg
      541 acgcaggcag atgacgcctg gcagctgagc ggcacgctcg atctgggcgt cgacgtgagc
      601 gtgccgcacc cgtcgcaatt cgatgcttcg atcgagctgc cggaaggtgg ggagagcgtt
      661 gatgtcgttt cggcgacgct ggttgcccca gagccgactc ctggcacctc acccttcggc
      721 agcgaatggc gtggccatgt ctcgcagcgc acggaggtgc cccatgacgg cgtgtggacg
      781 gtggccgagg cagacctggt cttcaccctc gtgccacata ccgtgccgtc gccctactac
      841 gtctatgagc tgaccggcgg cacgatgttc ttctccgtca gcggagccga ttcggagggc
      901 tgtacgtttg agctgtcccc agtcgagata ccgatcactc ccgagatggc cgagtccgcc
      961 cagggcttca cgatcgacgc cagcggcgcg acgccgactt tcagggggtt tgtgcacgtg
     1021 gtggggccag tagtcgaggt gatgcagacc tgccccgcgc cgtatgagta cctgaccggc
     1081 ccctattcga cgagcgccac cggcatattc atcgacgtca gctcagatga ggggcgtgcc
     1141 gtgatcggcg atcgcatctc cgggacgagc ctcgacgggt cgaagacgtt cgatatctcg
     1201 agagccgact agctctcgcg acggccggcg ttagccggcg atggctgcgg cctccggcag
     1261 tgtggtctcc gcctgtgcgg gcagggcgcc agtcacgtag gcatcgatcg cggccgccgc
     1321 gcgcttgccg tcgcccatcg cgaggatgac cgtcgcggcg ccgcgcacga tatcgccacc
     1381 ggcgaacacg ccgggcagcg aggtcatgcc cgcaccgtca acttcgatgt agccccactc
     1441 gttgactttc agtccggccg cggacgtcgt caggatgggg ttgctgcgcg tgccgatcgc
     1501 gaccacgacc atgtcgcagc cgatgacgaa ctccgacccg gccacgggcg atggccggcg
     1561 ccggcccgac tcatcgggct cgcccagctc catgcgcacg cacttcaggc cggtgaccca
     1621 gccgttctcg ccgacgacct cgactggcgc cacctgcgtc tcgaacttga tgccttccgc
     1681 ctgggcgtgg tggacttcct cgcgccgcgc cggcagttct tccatgcctc gccggtagac
     1741 gatcgtcgcc tccgccgcac ccagccggcg cgccgtgcgc accgcgtcca tggcgacgtt
     1801 gccgccaccc acgaccgcga cgcgctggcc gtgcagcacc ggcgtatccg cgtcggcacg
     1861 gtacgcgccc atcaggttga cgcgcgtcag gtattcgttg gcggaataca cgcccttcat
     1921 gttctcgccg gggatatcca tgaacaccgg caggcctgcg cccacggcga tgaatatcgc
     1981 gtcgaacgtc tggcgcagct cgtcgagcgt gtacgtcttg ccgacgatcg cgttgggctc
     2041 gatcttgacg cccaactctt ccaggcgtgt gacctcgtca tgcacgatgt ccttgggcag
     2101 ccggaactcg gggatgccgt agatgagcac tccgccggcg gtgtggaacg cctcgaacac
     2161 ggtgaccgcg tggccgcgct tggcgagctc gccggcggcc gtcaggccgg ccggaccgga
     2221 gcccacgatg gccaccgatt tgccgctcga ggccggcgcg accggcgcgc tctcgcgatg
     2281 cttgttggcc cagtcggcta cgaagcgctc caggtagccg attgccaccg cgtcgccctt
     2341 cttgccgcgc aggcagaccg cctcgcactg cgactcctgc gggcagactc ggccggttac
     2401 gcacggcagc gcgttgtcgt cgagcagcga atcggccgcg gcgcgcatgt cgccgcgcgc
     2461 cagcaggtcg atgaagcggg ggatgttgac gcggaccggg caacccgtga tgcacaccgg
     2521 gttcttgcac tgcaggcagc gctccgcctc gagcatcgcc actcgctcgg gcaggcccag
     2581 gttgacctcg gcgaagttgt gcgcacgggc gtccgcgtcc tgctcgggca tgagttggcg
     2641 caggatcgtc atgcgctcct tgggggtgac cgcggcgcgc tcgcccagcg gatggtcgag
     2701 gcgatcctcg atcgggacga ggggctcgcc gacgggccgc gtggtctcgt cgttcatcgc
     2761 gcggttcctg ccggtgccgc aacggccgcg gcgttgacct cggcgatctg cggacccagc
     2821 tggcagtggc gcttctcgag cgcctccttc tcgaagtcgc ggtaggggct gagacgatcc
     2881 gatagcaggg caaaatcaac ctggtgtgcg tcgaactccg ggccgtcgac gcaggcgtag
     2941 agcgtcttgc cgccgacgct cacccggcag ccgccgcaca tgcccgtgcc atcgatcatg
     3001 atcgggttga gcgacacgac cgtctggatg ccggtcgggc gggtcatctc cgcgaccgcg
     3061 cgcatcatcg gcaccgggcc gacggcgtag accacatcaa tcgggccgtc ggcgatcaca
     3121 tcggccagcg cctgggtgac gaagcccggc cggccgtacg agccgtcatc ggtgcaggtg
     3181 atcacctcgc catagcgctt gagctcctct tcgaggatga cccactcgcg cgagcggccg
     3241 ccaatgatgg aggtgacgcc cacaccattg cgcgccagtc cctgggcgag cggcagcatc
     3301 actgccgtgc cgacgccgcc gccgacacac accgcgcggc catggtcgag caactccgtc
     3361 tcgcggccca gcggcccgac aacatctcga atggcctgac caacttcgag cgcgaccaga
     3421 tcctcggtgc tcttgccgat ggcctggatg atcaaagtga tcgtgcccgc ctccgggtcc
     3481 gcgtcggcga tggtcagcgg gatccgctcg gcgccctcag ccgcacggac gatgacgaac
     3541 tgcccggcct ggcggacctg ggcgatacgt ggcgcctcga gcaccaggcg ggtgacgttg
     3601 ggcgatagct gtgatttccc ggtgatcggg tgcatctgtc ctccaagcgc agccggacaa
     3661 atgcatagtc agtcggtagg tgatttggcc gatacatcgg cagggcctat ttgagcgggc
     3721 cgaatgtcac tccgctgcgt gccgagcggt cctgcgcgcc gcgctgggaa gcgggatcag
     3781 ctcgcgctgg tcgccgtggt agcgggcgcc gccggcgcca cctcggccgt cttgcccttg
     3841 ggccagtaga gctccttctt gacgtggaac tccgggaagc cggcattcat gagcagccct
     3901 tcgacgttca ggatcatgtc cggattgccg cagatgtaga caacggtctt ctccggccgc
     3961 aggtgcaggt cgcggcacac gtcgagcacg acaccttcga cgcgaccggt gcggccttcc
     4021 cagccggcgt tgcgcggatc cgacgggcgc gagatcgtgg gcacgtaggt caccggatag
     4081 gtcttgtcgc gctcccatga ctcgagcacg tcgcggtagc ccagctcgtc gacgtaggac
     4141 gagccgttga ccatcaccac ccggcggggc tggcggtgga tcaggccctg gcgcatcatc
     4201 gagatgaacg gcgcgatgcc ggtgccggtc gaaacgaaca ggtgcgtccg ctcatcgttg
     4261 ggctcgagca ggaaccgtcc cttgggcccg atcatccgca tggggtggtc gatcggcagt
     4321 cgccacagca gcgtggtgaa gcgcaggatg ggcacgaggc gaacgtagaa ctcgtagccc
     4381 tcgctgcccg cgacctccgg cggagacgcg accgagtagg gccgctggac gatcttgtcg
     4441 tcggtgtaca cgccgatggt catgtactgg cccgggtcga agtgaacggg gtcgccctcg
     4501 aagcgcaccc agaaggtagc cagctcgggg gtgtggtcca cccgcgcgat cagtcgggcg
     4561 ttgtactgct cgtgctcgag cgcgatcacg cgcgggtcgg agtcgtgatg ggtcatgcgt
     4621 cgcgcatggt acgcggcttc atctgggctg gtcgtcgtca tcgtcgtcga gatgaagcga
     4681 gtgcaggacg cgatgcagga agggcgccag gatcactccg gcggtgacca ggaagaacag
     4741 gccgcagtac aacgcgtaga cgccggcgaa cagcttggcg ccgtccgtga tcagcacgtc
     4801 gaccgggccc atgccgccga ggatcattgc cgcgttcagg aaggcgtcga cgaatgacag
     4861 gtgcgccagg aacatgtagc ccgccacgcc gacggcgagc gaaccaagca gcaatagccc
     4921 ggccagcccg gcgtggcgca ggacgcgcgc cacgaaggca cgccgcgaga gcattggctg
     4981 gtcgtaccgc tcgtacacct tgcgcggatg ctgtcacacg ccgcgtggtt cgtctcgacg
     5041 ttcccgccgg cccagctcac gcggggagcg tcggggatca gtcccccgtg gtggcgccac
     5101 ccgccatacg catgtacccg taggccatga ccagcacgcc gatggcgacg acgatcgccg
     5161 ggacgaccca cttcgatgca tccgtatcga acaggtccag taccagaccg atgccgacga
     5221 gggacaggat gccgcccggg atcagcggcc agaaatgatg ctccttgagc cggaagagcc
     5281 agctcactcc ccagatgccg aggaagccga aggcgaggcc cagcacagcg ccggggccgt
     5341 catcctttgt gccgggcagc aggtcagcca ccaggaggcc cgccccgacc ccggtcagga
     5401 tgccggcgaa gacgagcgcg acatacgagc gggtcatgag gaatacggcc aacaggccaa
     5461 gaccgatcac gagcggaacg tagcggtcaa ggtcgggcag ccactgggat gccagggcgc
     5521 cgccgccgac gagcacgagg acgaaaccga cgatcagcgc cgtgcgcgac tggccccgcc
     5581 gagtcggagc ggcaatcgga gcagacatag tcatctgagt gcatgcctcc tgaagcctga
     5641 ttggccaccg tcagggtcgc tcgcagacct cagcggccac cagtgccaat cggaccattc
     5701 cgggtccgcg cgtacaatcg gagcctcgaa tatcaccgac agccgcgatg tcagtggctc
     5761 cccagcagcc tccgaggtca tcccaaatga cgcagtcccc gaccctgcct gccgaacgcg
     5821 cccacagggc cgccaagcgc tacatctatg ccttcggcgg cggccgcgcc gagggtgacg
     5881 ccaagatgcg cgaccagctc ggtggcaagg gcgcgggact ggccgagatg acgaacgccg
     5941 gcctgccggt gccgcccggc ttcacgatca cgaccgaggc gtgcaacgac tatttcgccg
     6001 cgggcgagaa gctgcccgac ggcctgtggg acgacgtgct cgcggcggtc gctcaggtcg
     6061 agcgcgagac cggcaagggc ttgggcgacc cggccaaccc gctgctcgtc agcgtgcgtt
     6121 caggtgcgaa gttctcgatg cccgggatga tggacacggt tctcaacctg ggcctcaatg
     6181 aatcgacctt gaatgggctg gtcgcgctga ccggcaacga gcgtttcggt tgggatgcat
     6241 accggcgctt catccagatg ttcgggcgca tcgtcatgga cgtcgacggc acgaagttcg
     6301 aacacgcgat cgatgccgcc aaagaggcac ggggcgtcaa gcaggacacc gagctggacg
     6361 ccgacgcact gcgcgagctg gtgggcacct tcctgcgcgt ggtcaaggag tcgaccggac
     6421 gcgaattccc gaccgacccg cacgagcagc tcgatctggc catcaaggcc gtcttcgcat
     6481 cgtggttcgg caagcgggcg cacgactatc gcgagtacaa ccacattgcc cacgacctcg
     6541 gcaccgcagt caacgtggta acgatggtct tcggcaacat gggcgacgac tcgggaaccg
     6601 gcgtagcgtt cacgcgcgat ccgaacacgg gcgagaaggt cctcttcggt gagtacctga
     6661 ccaatgccca gggcgaggac gtggtcgccg gcattcgcac gccgcagccg atcagccaca
     6721 tgaaggacga gctgcccgag gcctacgccg agttcgagcg catcggccag cgtctcgaga
     6781 agcactacaa ggacgtgcag gatctcgagt tcaccatcga gcgcggccgc ctctacatgc
     6841 tccagacgcg gtcagcaaag cgcacggccg cggccgcggt ccgcatcgct gctgacctcg
     6901 ttgaagaggg catcatcacc gagcgagagg cgctgtcgcg catcgagccg gcgcacgtcg
     6961 accagctgtt gcgcgaccaa ttcgacccag ctgccaagac gggcgccaag aagatcaccg
     7021 cgggcctgaa cgcgtcaccc ggcgcggccg tgggcaaggc cgtgttcgat gcggatcgcg
     7081 cggccgagat ggccggccag ggcgaaaagg tcatcctcgt gcgcatcgag acgtcgcccg
     7141 acgacttcca cggcatggcc gcggcgcagg gcgtgctcac cgcgcgcggt ggtgcgacat
     7201 cacatgccgc cgtcgtggcg cgccagatcg gtaagccgtg cgtcgctggt tgcgcggcgc
     7261 tcgtcatcga ctacgggacc aagtccgcgc gcgtgaatgg cctcggcttt tccgaagggg
     7321 agtggatctc cctcgacggc accacgggtg aggtgttcgt gggcgcgctg ccgacgaccg
     7381 cggcgcgctt cgaggaccag gccgagcttc agcaggtgct cgcgtgggcc gacaaggtgc
     7441 gccggctcga ggtctggacc aatgccgaca agcccgagga agcggcccag gcgcggcgct
     7501 acggcgcgca gggcattggc ctgtgccgga ccgagcacat gttccgcgag ggggagcggc
     7561 tggacatcgt gcgcgacgcc atcctcgttg ccttccaggc gactcgggcg aaggagaagg
     7621 cggccgccgg caccatcctg tccaccgacg acgccgccgc ggtagcttcg ttcgacgagg
     7681 caatcgccaa gctcgaaaag ctccaggaag gcgacttcta cggcatcttc gaggcaatgg
     7741 acgggctgcc cgtcgtcatc cgcctgatcg atccgccgct gcacgagttc ctgcccaacc
     7801 acagcgaagt gctggtcgag gtcacgcgca tggaggcgaa gggcgtcccc gccgacgacg
     7861 cggactatgt caaggccaag gagctgctac gcgcggtcga ggcgctgaaa gagcagaacc
     7921 cgatgctcgg cctgcgcggc atccgcctcg ggctgatgat ccccgacctc atcaagatgc
     7981 agacccgcgc catcctcaac gccgccatcc gccgccagaa cgccggcggc catccgcacg
     8041 ccgagatcat gatcccgctc gtcggccacg tgaacgagct cgctgcgacg cgcaagatcc
     8101 tcgacgccga ggcgaaggcc gtccaggagg ccgctggcac aaccgtcgac ttcatgttcg
     8161 gcacgatgat cgagatcccg cgcggcgcgc tgacggccga ccagatcgcc gagcacgccg
     8221 agttcttcag cttcggcacg aacgacctga cgcagatggc cttcggctac tcgcgcgacg
     8281 acgcggaggg cggcttcctc ctcaactacg tcgagcgcgg catcctgccc gacaacccgt
     8341 tccagacgct cgacaccgtc ggcgtcgggc agctcgtgcg catcgcggtc gagaggggtc
     8401 gggccgcacg accgggcctc aagatcggca tctgcggcga gcatggcggc gaccccgcgt
     8461 cgatcgcttt cttcaacgag gtcgggcttg actacgtgtc gtgctcgccg ttccgcgtcc
     8521 ccgtggcgcg tctggccgcg gcgcaggccg cactctccgg ggcggccgag cgcgacaagt
     8581 aatgaacgag cccccggtcg tctggcatta cggccttatg gccgatcact gggcggagtt
     8641 caacacggcc acgcctgagc tggagttcat tcgcgaggcg gtaagcaagt acggcgagcc
     8701 cgtgctcgac cttgcctgcg gagccggtcg cctcctcgtc ccgctgctgt cggccggagt
     8761 ggacgttgac ggcgcggaca tctcatcgga catgattgag cgctgtcgcc aggcggcggc
     8821 gcgggtcggc aggagcacga atctgtactg ccagcagatg gatcaaatcg atctgcctcg
     8881 ccgctatcgg acgatttacc tgtgcggctc gttcgggctc gccggtagtc gtcagcgcga
     8941 cctcgaaacg ctgcgccgtt gccgggcgag tctcgcggat gggggtgcgc tcattttcga
     9001 catcgacgcg gagtacgcgt cacctgaatc ctggaacaag tggctacctg agcatgccag
     9061 caggctgccc gaggcgtggc ctaaaagtgg ttcgcgccag acggatgctg acggcgctga
     9121 gcatcgcgct ttcttccggg ccatcgagga cgacccgctc gaactgacct atacccgtga
     9181 ggtgcgcctc gagaggtggg aaaagggttc gctggttgcc tcagagcagt attcgctgcg
     9241 cgggaatgcc tatctcaaac cagaggtgga gttgatgctg cgtgtcgcgg gcttcggcga
     9301 cgttgacgtg cgcggtgatt ggagggagca gccggcgtcg gtgaccagcg agcaggtcgt
     9361 gttcatcgca cggacatagt tcgctgggaa aagagatgag cctcgatctc tcgatcgagg
     9421 ctcatccagt agcgacggaa acgatgccgt caggcggggt tggctaaggg ttaggcgcgg
     9481 cggcgctgga aacagtcgtc gcagaagacg ggcttgccgc tcgtcgggcg gaacggcacc
     9541 tgtgccgtct tgccacagtt cgagcaggtg gcatcgaaca gctcgcgcgg acggcgctcg
     9601 cggaaaccgc caccgccgcc ggcgctgtag ccgccgccac cacccatgga atcgccagag
     9661 cccatcgacg ccttgcgggc ggcgcggcat gagcggcagc ggcgcgggtc agagaaacca
     9721 cgctcctggt agaactgctg ctcactggca gtgaaaacga actcctggcc gcagtcggca
     9781 caggtaagcg ttcgatccgc ttgcaaagaa aaaactcctt ctcgagccgg gcttcgcgac
     9841 ttagttctcg cgtaagaccc gtgagaggag cgtcgtggtg gaccggctat tacacccgta
     9901 gcgacgggct tcgccttaat cgtacccgaa agcctgatgc ttacgcaatg acctccgatc
     9961 agcccagc
//
LOCUS       DATBRX010000068         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_468240, whole genome shotgun sequence.
ACCESSION   DATBRX010000068 DATBRX010000000
VERSION     DATBRX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468240"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VIF08_02400"
     CDS             complement(<1..746)
                     /locus_tag="VIF08_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134863.1"
                     /translation="MLARSAVLRDFTRRDGVRLVAFGIALELILGTILAISAFLDPFG
                     NRIPRVAGWYLLAALVVALLLAWIWRFRPQIWNRSNALWLIALLLIGTTIAMKITAGR
                     PVLPYVLPTMAVGLLLAVLLDAGTALLVTGMLALLGGAITGSVEFAAYGLLGGMAGIV
                     LVRRGERFGHFLQAAIAIAIVNIGVVASWALLGNRDLTGLAELAGAAFASAAGSALAA
                     LGSFVVVGTLFGITTSFQLLELANPTQKLLR"
     gene            complement(759..1229)
                     /locus_tag="VIF08_02405"
     CDS             complement(759..1229)
                     /locus_tag="VIF08_02405"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY8134864.1"
                     /translation="MTLQNRLQQDMQAAIRERDELRRDTLRMVVSAAYNAQKHAGREL
                     TDDEVITVLAREVKTRRESVEAYTSGGRADAAAKEQAEIAIISAYLPEQIGAEELAQL
                     VATTVEESGATSVRDVGKVMAVLMPRVRGRADGKQVSALVAQELTKRDLAGHGH"
     gene            complement(1266..1445)
                     /gene="rpsU"
                     /locus_tag="VIF08_02410"
     CDS             complement(1266..1445)
                     /gene="rpsU"
                     /locus_tag="VIF08_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY8134865.1"
                     /translation="MAEVRVGENESFESALRRFNKKIQQSGILAEARRREHYEKPSVK
                     RKRKEAKRKKATREG"
     gene            1572..>2710
                     /locus_tag="VIF08_02415"
     CDS             1572..>2710
                     /locus_tag="VIF08_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8134866.1"
                     /translation="MGSWDLPDPRTFDSIIDVIEDAATRWAGREQMALRTDDGLHLPW
                     TAADINYHSKLVAWRLKRLGLEEGDRLLTWSPSTPALPALYWGAMRARVVIVPLDLRM
                     APDVLQRIATASDAKWLALGTGLDAPDPQASGLGHLNVRTVEWLAAEPAHERADHTDE
                     GGLDDPFPVDWPAQVDAWPRPTRDSLFEVIYTSGTTGNPKGVMLKHGTILSTLEGITR
                     ILPPREHRTVSLLPPSHLFEQAPVLMFGTMIGAHILFVRSRNPRVIFDALKEERVTTM
                     VLVPQLVELFWLGLMREVKRQGKEKTFERARRIARLLPYWARRLIFRSVHKQLGGALN
                     LMVSAGAYLPPALQQSWEDLGVIVLQGYGATECGPAAATSEQDHPT"
BASE COUNT          437 a          954 c          898 g          421 t
ORIGIN      
        1 cgcagcagct tctgggtggg gttggccagc tccagtagct ggaacgaagt ggtgatgccg
       61 aagagggtcc cgaccaccac gaaggagcca agcgccgcga gggcgctgcc tgccgcggaa
      121 gcgaacgcag cgccggccag ctccgccagg ccggtgagat cgcggttgcc caggagcgcc
      181 cacgacgcga ccacgccgat gttgacgatc gcgatcgcga tggcggcctg caggaagtgg
      241 ccgaagcgct cgccgcggcg caccaggacg atgcctgcca tgccaccaag cagcccgtag
      301 gccgcgaact cgactgagcc agtgatggcg ccgccgagca gggcaagcat gccggtgacc
      361 agcaaggccg tgcccgcgtc gagcagcacc gcgagcagca ggccgactgc catcgtcggc
      421 agcacatacg gcagaaccgg ccggccggcg gtgatcttca tcgcgatcgt ggtgccgatc
      481 agcagcaggg caatcagcca cagcgcgttc gagcggttcc agatctgcgg ccggaagcgc
      541 cagatccagg cgagcaacag cgccacgacg agcgccgcca acaggtacca gccggcgacc
      601 cgagggatgc ggttgccgaa tgggtcgagg aaagcgctga tcgcgaggat cgtgcccagg
      661 atcagctcga gcgcgatccc gaacgcgacg aggcgcacgc cgtcacgacg ggtgaaatcg
      721 cgcagcaccg cggaccgagc cagcatcttc tgtgggcctt agtggccgtg gccagccagg
      781 tcgcgctttg tcagctcctg ggcgaccagc gccgagacct gcttgccgtc cgcccggccc
      841 ctgacgcgcg gcatgaggac ggccatgacc ttgcccacgt cgcgcacaga ggtggcgccg
      901 ctctcttcca ccgtggtcgc gaccagctgg gcgagctcct ccgcgccgat ctgctcgggc
      961 aggtacgcgc tgatgatcgc gatctctgcc tgctccttgg ccgcagcgtc tgcccggccg
     1021 ccggaggtgt acgcctcgac cgattcacgg cgcgttttga cctcgcgcgc gagcacggtg
     1081 atcacctcgt catcggtgag ctcacggccg gcgtgcttct gggcgttgta cgcagccgaa
     1141 accaccatcc gcaacgtgtc gcgccgaagc tcgtcgcgct cacggatggc cgcctgcatg
     1201 tcctgctgga gcctgttctg gagagtcacg gctttgcgtt cctgggggcg atgccgccca
     1261 gccggctaac cctctcgggt ggccttcttg cgcttcgctt ccttgcgctt gcgcttcacg
     1321 ctgggcttct cgtagtgctc acgtcggcga gcctcggcga ggatgccgct ctgctggatc
     1381 ttcttgttga agcgtcgcag agcgctctcg aaggactcgt tttcgcctac gcggacttcc
     1441 gccaaaccag gatcacctcc cacgggccca atgtcagacg ctcatctttg tgactcgccg
     1501 cgctcgtgcg cgaccgcgtc tggggctgcg gaagtgtacc cgaaggaggc gaatccatag
     1561 actccgcggc gatggggagc tgggacttac ctgatccacg gacgttcgat tcgatcatcg
     1621 acgtcatcga ggacgccgcg acgcgctggg cgggacgcga gcagatggcg ctgcgcaccg
     1681 acgatgggct gcacctgccc tggaccgcgg ccgacatcaa ctaccactcg aagctcgtcg
     1741 catggcgcct gaagcggctg gggctcgagg agggtgatcg gctactcacc tggagtccgt
     1801 cgacgcctgc gctcccggct ctctactggg gcgccatgcg cgcacgggtc gtcatcgtgc
     1861 cgctcgacct ccggatggcg cccgatgtcc ttcagcgaat tgcgacggcc agcgacgcga
     1921 agtggctggc ccttggcacg ggcctcgacg ccccagaccc acaggcatcc gggctcggcc
     1981 atctgaacgt ccgcaccgtc gaatggctcg ccgccgagcc cgcgcacgag cgcgccgacc
     2041 acaccgacga gggcggtctc gacgatccct tcccggtcga ctggccggcc caggtcgacg
     2101 catggccgcg acccacgcgc gactcgctct tcgaggtcat ctacaccagc ggcacgacgg
     2161 gcaaccccaa gggcgtgatg ctcaagcacg gcacgatcct ctccacgctg gagggcatca
     2221 cccgcatcct gcctccccgc gagcaccgca cggtcagcct gctgccgccc agccacctgt
     2281 tcgagcaggc gccggtgctg atgttcggca cgatgatcgg cgcacacatc ctctttgtcc
     2341 ggtcgcgcaa cccgcgcgtc atcttcgacg cgctcaaaga ggagcgcgtc acgacgatgg
     2401 tcctcgtgcc gcagctggtc gagctgttct ggctgggcct gatgcgcgag gtcaagcgcc
     2461 agggcaagga gaagaccttc gagcgcgccc ggcgcatcgc gcgcctgctg ccctattggg
     2521 cgcgccgcct catattccgc agcgtccaca agcagctcgg cggtgcgctg aatctcatgg
     2581 tgagcgcggg tgcctatctg ccgcccgcgc tccagcagtc ctgggaggac ctgggcgtga
     2641 tcgtcctcca gggctacggc gcgaccgagt gtggtcccgc ggcggcgacg agcgagcagg
     2701 accaccccac
//
LOCUS       DATBRX010000069         4667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2367827, whole genome shotgun sequence.
ACCESSION   DATBRX010000069 DATBRX010000000
VERSION     DATBRX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367827"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VIF08_02420"
     CDS             <1..861
                     /locus_tag="VIF08_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134867.1"
                     /translation="AKVAQSGWGAQLLARQESDGVWRWSGWDPESGEWGGSPPWITLL
                     SLGLLKSLAPDPADPTVAAAMARLVAGGFRSSMPDFFAWAGRGFFEGETEACINGRVV
                     GIGAYFRQDVHAVVERLLGEQMADGGWNCELENGSTRGSFHSTVCVLEGLLEYERSGL
                     ARHATDDARRRGEEYLLERHLLRRLSTGEEVSEHFGRFGFPYAYRFNVLRGLDYLRAA
                     GAPRDPRMDEALQLIRERRGSDGKWLLENSHEEEWVLDMGEAEGEPSRWVTLHALRVL
                     RHFSALPGIS"
     gene            867..1637
                     /locus_tag="VIF08_02425"
     CDS             867..1637
                     /locus_tag="VIF08_02425"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEY8134868.1"
                     /translation="MRGPGSLHVITGCMFSGKTDELVRQLRRAAVGGRSVMLIRPSAD
                     TRSPAEVAESRSGARFDSIAVASANDIIPTVMAAPAEVIAIDEVEFFGFDVADVADRL
                     ATDGYEVIVSGLDTDFAGRPFGALPNLLALADSVTKLTAVCVFPGCGAEATRTQRLVN
                     NEPAAIDDPLIVVGGTETARAPDRYEARCRTHHRVAPPRHGTATIKSNATANPELTEL
                     AQEMSPDMAHEMTSAMPEPAASNGNGHEDFNLSELPAP"
     gene            complement(1672..2577)
                     /locus_tag="VIF08_02430"
     CDS             complement(1672..2577)
                     /locus_tag="VIF08_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134869.1"
                     /translation="MLSQIGHAVLWATGTAFPQIEPELIVAPVMAGMLLALSVFMRRT
                     ADAAFDDFRPALANPDVEEVERHRLLSIPDRAFLASAVVITVMTTALYLAFVGPSATP
                     RTPTADSVITAGWIVFSVMLGLVISQIVSQLRSVRHLSEIAQNIDVLNPGPVDALSRV
                     TAVGGIGMLTFLAVANIGLPGSASGYVALDVGIIVFALVAFLLPLQVMHRRLDRQKAD
                     LLAASRVRLKSVLGALHSAVDAHDYTSADALNKMVATTIAERDLLMRLQTWPWTPTTI
                     RGFASALLLPVIIFVITRGIDRLLV"
     gene            complement(2662..4278)
                     /locus_tag="VIF08_02435"
     CDS             complement(2662..4278)
                     /locus_tag="VIF08_02435"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HEY8134870.1"
                     /translation="MLVALLALAALAQPALADEPAPTPLPEALSGPDANSLFRTTSTY
                     GLSCQSFSAPPLGTQWACGREVAPAVWYRARFAISDPLIMELHVDGPVPWPLDHDQYL
                     QQMTSWFCDAGTTDLVLAFLAQGRVQPEGAVYEDASCRLFSDYTTDPIPVQHVIVWAK
                     KTSDVTVVPTATPFNEFTLPPIPTAPPPLATSTASPTPGISGTPIPQPTLPTGGGEGG
                     SPGPGRFVTSIAVPAALSADPLVLLQSMGLALLIVLLMPFPAQLFNSTYETHAGYIRR
                     FFRLDTLQRAMGPLSGFWESLVGILLFVLLSGVVYALLDPNLSLSGSGLATILGLALG
                     ILLTGLLVSAPSLINSIRTGERFKMRVLPATLLVGLACVVVSRLTGFLPGYVYGVILG
                     FVFARELSRMEQGRANALAALAMLAVALVSWLLLGFVANAGLGLIGIVVSTLCATLLV
                     ACLEGVVFGLLPFRFLPGEPLYATNRLIWAALLFVGAFCFFHILINPASGYMGSTATT
                     PLFTMVALLVGFGALSIAFWAYFRNKPVEA"
     gene            complement(4458..>4667)
                     /locus_tag="VIF08_02440"
     CDS             complement(4458..>4667)
                     /locus_tag="VIF08_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134871.1"
                     /translation="DLCHATSPAAVSLVGQGGPQGNPKGPRDANDPGSTRYGKRSGAG
                     GNEPKESETKQVDGWNSRQGGDQSR"
BASE COUNT          843 a         1510 c         1619 g          695 t
ORIGIN      
        1 gcaaaggtcg cccagtccgg ctggggcgcg cagctgttgg cgcgccagga gtccgacggc
       61 gtatggcgct ggtcgggctg ggaccccgaa tcgggcgaat ggggcgggtc tccgccctgg
      121 atcacactgc tgtcgctcgg cctgctcaag agccttgcgc cagatccggc cgacccgacg
      181 gtcgcagcag caatggcgcg actggtcgcg ggaggttttc gatcctcgat gcccgacttc
      241 ttcgcatggg ccggtcgggg cttcttcgaa ggcgagaccg aggcctgtat caacggccgg
      301 gtcgtcggga tcggcgccta cttccgccag gacgtccatg cggtcgtcga gcgactgctc
      361 ggcgagcaga tggccgacgg aggctggaac tgcgagctcg agaacgggtc gacgcgtggc
      421 tcgttccact caacagtttg cgtcctcgaa ggcctgctcg agtacgagcg atcgggcctt
      481 gccaggcacg ccacggacga tgcccggcgg cgcggcgagg agtacctgct cgagaggcac
      541 cttctgcgcc gcctgtccac tggcgaggag gtcagcgagc actttgggcg ctttggattt
      601 ccctacgcct accgcttcaa cgtgttgcgc gggctcgact acttacgtgc tgcaggtgcg
      661 ccacgggacc cgcgcatgga tgaagcgctc cagctcatca gagagcgccg cggttcggat
      721 gggaagtggc tgctcgaaaa ctcccatgaa gaggaatggg tcctcgacat gggcgaggcg
      781 gagggcgagc ccagccgctg ggtgacgctt catgcgctgc gcgtgctgcg ccatttcagt
      841 gcgctacccg ggatatcctg acggccatgc gtggacccgg ctcgctgcac gtcatcaccg
      901 gctgcatgtt ctccggcaag accgacgagc tggtgcgtca gctgcgccgc gcggccgtgg
      961 gcggccggag cgtcatgctg atccggcctt cagctgacac ccgatcgccc gcagaagtcg
     1021 ccgagtcacg ctccggcgca cgcttcgact ctatcgccgt ggccagcgcg aacgacatca
     1081 tcccgaccgt catggccgcg cctgccgaag tcatcgcgat cgacgaggtc gagttcttcg
     1141 gcttcgacgt ggctgatgtc gccgatcgcc tcgcgaccga cggctacgaa gtcatcgtgt
     1201 cgggtctcga cacggacttt gcgggccggc ctttcggtgc gctgcccaac ctgttggcgc
     1261 tcgctgactc ggtcacgaag ctcaccgccg tgtgcgtctt tcccggttgc ggtgcagagg
     1321 cgacgcgcac gcagcgcctg gtcaacaacg agccggcggc cattgacgat ccgctcatcg
     1381 tcgtcggtgg cacggaaacg gcccgcgcgc ccgatcgtta cgaggcgcgc tgccggacgc
     1441 accaccgggt ggcgccgccg cgccacggca cggcgactat caagtcgaac gcgacggcca
     1501 atccggagct gaccgagttg gcgcaggaga tgtcacccga catggcgcac gagatgacat
     1561 cggcgatgcc cgagccggcg gcatcaaacg gcaacggcca cgaggacttc aacctctcgg
     1621 agctgcccgc gccgtaggcc cgcaagcgga gcggcgcgga gacagacgct ctcagaccag
     1681 cagtctgtcg atgcctcgag tgatcacgaa gatgatcacc ggcagtagca gcgccgaagc
     1741 gaaaccgcga atagtcgtcg gcgtccacgg ccacgtttgc aggcgcatca gcaggtcgcg
     1801 ctcggcgatc gttgtggcga ccatcttgtt gagtgcgtca gcactcgtgt agtcgtgcgc
     1861 atcaaccgca gagtggagcg cgcccagcac ggacttgagg cgcacgcggg aagccgcgag
     1921 gaggtccgct ttctgtcgat cgaggcgtcg gtgcatcact tgcagcggca gcaggaacgc
     1981 gacgagggcg aagacgatga tgccgacatc cagcgcgacg tagcccgacg cggaacctgg
     2041 caagccgata ttcgcgacag caagaaatgt cagcatgccg atgccgccga ccgccgtgac
     2101 gcgcgacagc gcatcgaccg ggccggggtt gaggacatcg atgttctgag cgatctcgct
     2161 gaggtggcgg acggaacgga gttggctgac tatctgtgag atcaccagtc cgagcatgac
     2221 cgagaacacg atccagccgg cggtgatcac gctgtcggcc gtgggcgtgc gcggcgtggc
     2281 cgaagggccg acaaaggcga ggtaaagagc ggtggtcatg accgtgatca caactgcgct
     2341 ggccagaaat gcgcgatcgg ggatcgacag gaggcggtga cgctcgacct cctccacatc
     2401 cgggttcgcc agggccggcc ggaagtcgtc gaaggcggcg tctgccgtgc ggcgcatgaa
     2461 cacgctcaag gccaggagca tgcccgccat gacgggcgcg acgatcagct ccggttcgat
     2521 ctgcggaaac gccgtccccg tggcccagag cacggcgtgg ccgatttgag acagcaagac
     2581 tcccgctcca gcgaaccagg cttcgggcgg gcccggcagg cgttcaaccc aattcaggac
     2641 gcggtcgatc acggcgattg gctacgcctc gacgggcttg ttcctgaaat acgcccagaa
     2701 ggcgatcgag agcgccccga acccgacgag cagagcgacc atcgtgaaca gcggcgtggt
     2761 cgcggtcgag cccatgtagc ccgacgcggg gttgatcagg atgtggaaga agcagaaggc
     2821 gccgacaaac agcagcgctg cccagatcag ccggttcgtc gcgtacagcg gctcgcccgg
     2881 caagaaccgg aatggcagca ggccgaacac gacgccctcg agacaggcca ccagcagcgt
     2941 cgcgcacagc gtgctcacga cgatgccgat caggcccagt ccagcgttcg ccacaaaccc
     3001 gagcagcagc cacgaaacga gggccaccgc gagcatcgcc agcgcagcca gggcattggc
     3061 gcgaccctgc tccatgcgcg acagctcgcg cgcgaatacg aagcccagga tcacgccgta
     3121 cacgtagccc ggcaagaagc cggtcagtcg gctgaccacg acgcacgcca ggccgacgag
     3181 cagcgtcgca ggcaggaccc tcatcttgaa ccgctcgccc gtgcgaatgc tgttgatcag
     3241 cgacggcgcc gacaccagca gcccagtcag caggatgccc agcgccagcc cgaggatcgt
     3301 cgccaggcca gagccgctga gcgacaggtt cggatccagc agcgcgtaga ccacgccgga
     3361 tagcagcacg aacagcagga tgccaacgag actttcccag aagccgctga gcggtcccat
     3421 ggcgcgctgc agcgtgtcga gtcggaagaa gcggcggatg tagcccgcgt gcgtctcgta
     3481 ggtcgagttg aagagctgcg ccgggaaagg catcagcagc acgatgagca gcgcaagacc
     3541 catgctctgc aacaggacca gtggatcggc cgacagcgct gccggcacgg cgatggaggt
     3601 tacgaacctg cccggacccg gggagccgcc ttcgccacca ccagtgggca gcgtgggttg
     3661 ggggatcggc gtcccggaga tgcctggagt tgggctggcg gtcgaagtgg caagcggggg
     3721 aggggcagtt gggatgggcg gcagcgtgaa ttcgttaaaa ggcgtcgcgg tcggcacgac
     3781 cgtgacgtcg gaagtcttct tggcccagac gatgacatgc tgaaccggta tcgggtcggt
     3841 cgtgtagtcg gagaagaggc ggcatgacgc atcctcgtag accgcgccct cgggttgcac
     3901 gcgcccctgc gccaggaagg caaggacgag gtccgttgtc cccgcgtcgc agaaccacga
     3961 cgtcatctgc tggaggtact ggtcgtggtc gagcggccat ggcacaggac cgtcgacgtg
     4021 cagctccatg atcagggggt cactgatcgc gaaccgcgcg cggtaccaga ccgctggcgc
     4081 cacctcgcga ccgcacgccc attgggtacc gaggggcggc gccgagaacg actggcagct
     4141 gagcccatac gtgctggtcg tgcggaaaag ggagttggcg tccggcccgg acagcgcctc
     4201 ggggagggga gtcggcgcgg gctcgtcggc cagggcaggc tgggccaggg cggccagagc
     4261 cagcagagcg accagcatcg cgccagtcgc cagggacacg cgggtgcgcg cgtgcagcat
     4321 ccgcggatga tgcacccccg atgggccggc cgctagcctc cggcgacacc tccggcccca
     4381 aagtcggcta cccctagcct cgcaggcgcg aattcaccac tgctagtgtc accggtgctt
     4441 cgctcggcgc gcgggtatca gcggctctga tcgcctccct ggcgggagtt ccagccgtcc
     4501 acctgcttcg tctcgctttc cttgggctcg ttgccaccag cgccgctgcg ctttccgtag
     4561 cgcgtcgagc cggggtcgtt ggcgtctctg ggtcccttgg gatttccctg tgggccgccc
     4621 tggcccacga gagataccgc ggcgggtgag gtggcgtgac agaggtc
//
LOCUS       DATBRX010000070         5506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2479714, whole genome shotgun sequence.
ACCESSION   DATBRX010000070 DATBRX010000000
VERSION     DATBRX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2479714"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..872
                     /locus_tag="VIF08_02445"
     CDS             108..872
                     /locus_tag="VIF08_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY8134872.1"
                     /translation="MNDAELAAREVEIPNTGATVHDVTFDEAVELIVRWAREGSGGYV
                     STPNVDHLVRAHREADFRALMRGARLRLPDGMGLIYGMRLRGRRLKGAVHGRLLPEAI
                     VRTAGDSVALAMIGGRDDAPARASERLTELGGKVVAAVSPPMGFEIGGAADEEATRGL
                     VAAEPRVVIVGFGAPKQERWMARHAAEFPHTVMLGLGQTIDVLGGRVPTAPAWMTRVG
                     LEWAFRMLRDPVRIGRRVFVDDPPFFWWMVRARLGR"
     gene            897..1967
                     /locus_tag="VIF08_02450"
     CDS             897..1967
                     /locus_tag="VIF08_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8134873.1"
                     /translation="MSRPRRIAFYLALAFVMALAGASPVAAQVRAFDAMPWIGLAAKF
                     AAPLFPSAVSKGSDNRLTVMLVGSDWRPRLAGTGERTDSIIFMTIKDGQISAVSLPRD
                     VGNVPIAPGVIFKPKINGLFKYYKSQSGLSDPLAARNWALQKMRTAFQYTFGIQIDYV
                     VYIRFTGFERLVGNVSGVPVNVEKTIYDKKITDDRYPNNQRGAKFLAQVTTELGSSAP
                     ACYTVGSPVNWNASPNCTRALEYVRSRHGPGNNDWVRGRRQQSFIFAAIKRVISRGSG
                     ANFNSLRTSALSNSTDFYTTLPTDTGSALELFNLLAGATMPNQAVLKPTTYASTVAGT
                     SKQQLKIEVVRSLMRSWFGPIN"
     gene            2101..3492
                     /locus_tag="VIF08_02455"
     CDS             2101..3492
                     /locus_tag="VIF08_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8134874.1"
                     /translation="MLIRQFIVDGLGHISALVADDARGVAAVIDPRRDVDVYLAAAAE
                     RGWRINHVLETHLHNDYISGGPELAALTGARHVIGAGAETAYEHQPTRDGDAIDIGSL
                     RFTTVDTPGHTPEHVAYTLADRGRADDPLAVFTGGSLLVGAVGRTDLLGADNAEQFAR
                     QMFHSLHDKLLAHNDYVGVLPTHGGGSLCSKDISSTPSSSIGFERRHSELLAIPAIED
                     FVRALLFDQPGYPRYFARMRPINKAGATPLGTIPGPRELTVAQVQALLADDGLVIDAR
                     PYPDYAAGHLPGSFAIPAGSSFGTWLGWVVPDGRPLVFVLESAADWDDLLRQALRIGY
                     DDVRGHLSSVADWRAAGLPLETGKRANIEELATLLNRGGPVAPLVLDVRQHNEWVEGH
                     IPGSVHLMGGDLPDRLAELPRDRPIMTICRTANRSAIAAGVLLRAGFSDVTWIDSGVP
                     AWRRAGFPIEKGE"
     gene            3600..4886
                     /locus_tag="VIF08_02460"
     CDS             3600..4886
                     /locus_tag="VIF08_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134875.1"
                     /translation="MNKSSFVLVLAFSFAGGLPAARPVDAASVSSLNLRATYEVSASF
                     SWATRAVSVHTTAHVTNPTGSAVSTVAFNLGTLHTGNANVGVVTVRGSAVSETIKDQT
                     VLVPFSPPLGAGSSADVVINYNARLASKAGGYKWQFVRRAGVMTAYRWVPWLSRTARF
                     ANAGGGEAYVTPSSASVEVAITTDQPLVIASSGQRIAVNGLTQTFVAHDVRDFNFSAA
                     PDYQTATRVTHGTTIKFFYRTLPPSTVLDKAVASFNHLSDNVGAYPEDFLNIAEVGPW
                     YGMESPNLNWIPSDAGGLLNYMVAHEVAHEWFYSTVGNDQALEPFADEALSEFLGREI
                     AGTWTSSKCPNGRLDRTIYTTPGDCYVAVVYYQGYRYLDAYRDHVGNSDFYQGLRNYY
                     QANKFGMGGTRKLLNALDAASGYHPNHAARFPTLYP"
     gene            4895..>5506
                     /locus_tag="VIF08_02465"
     CDS             4895..>5506
                     /locus_tag="VIF08_02465"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY8134876.1"
                     /translation="MSPARRSAPPRPKRIDVPTRTEADSQAPALSEEITRAWDDPPYE
                     ERPRASRGDDVPLLGNDELARIFYEIGDMLEIQGEVVFKTGAYRRAADNIAHASVDVA
                     RAYRAGNPPRLAGVGKAIDEKLAELADTGRLRYYERLRQDVPPSVVTLLQVPGLGPRT
                     AGELWRQANISTLDQLEAAASAGTLRELKGMTARTEERLLTALK"
BASE COUNT          876 a         1916 c         1873 g          841 t
ORIGIN      
        1 gcgcgcatgg atccggcgct gcgtgccgcg ctgaccgccg agttcgcgcc gcagatcgcc
       61 gagctgggcg agatcatcgg gcgcgatctc tccgcgtgga gcagggcatg aacgacgccg
      121 agctggccgc gcgcgaggtc gagataccca ataccggggc gaccgtccac gacgtgacct
      181 tcgacgaggc ggtagagctc atcgtgcgct gggcgcgcga gggcagcggc ggttacgtct
      241 cgacgcccaa cgtcgaccat ctcgtacggg cgcaccgcga ggctgacttc cgggcgctca
      301 tgcgcggcgc acgcctcagg ctgcccgacg gcatgggcct gatctacggc atgcgcctgc
      361 gcggccgccg actgaagggc gcggtccatg ggcgcttgct gccggaggcg atcgtgcgca
      421 ccgccggcga ctcggtcgcg ctcgcgatga tcggcggccg cgatgacgcg ccggcgcgcg
      481 cgagcgagcg gctgaccgag ctgggcggca aggtcgttgc cgcggtcagc ccgccgatgg
      541 gcttcgagat cggcggtgcc gctgacgagg aggccacacg tgggctcgtg gcggccgagc
      601 cgcgcgtagt gatcgtcggg ttcggggcgc ccaagcagga gcgctggatg gcgcgccacg
      661 cggccgagtt tccgcacacc gtgatgctcg gcctgggcca gacgatcgac gtgctcggcg
      721 gccgcgtccc gaccgcgccg gcgtggatga cccgggtcgg cttggagtgg gcattccgga
      781 tgctgcgcga cccggtgcgc atcggccggc gcgtcttcgt cgacgatccg ccattcttct
      841 ggtggatggt gcgcgcccgg ctcgggcgct gattcacttg ggggataatg caaacgatgt
      901 cgcgacctcg ccgtatcgcg ttctatctcg cgcttgcctt cgtgatggcg ctggccggcg
      961 cgagcccggt cgctgcccag gtgcgcgcct tcgatgcgat gccgtggatc ggtctggccg
     1021 ctaagttcgc cgcaccgctt ttcccgagcg cggtcagcaa gggcagcgac aaccgcctga
     1081 cggtgatgct tgtcggctcg gactggcggc cccgtctcgc gggcacgggc gaacggaccg
     1141 actcgatcat cttcatgacc atcaaggacg gccagatctc cgccgtgagc ctgccgcgcg
     1201 acgtgggcaa cgtgcccatc gccccgggtg tcatcttcaa gcccaagatc aacggcctgt
     1261 tcaagtacta caagtcgcag tccgggctga gtgaccctct ggctgcccgc aactgggcgc
     1321 tccagaagat gcgcacggcg ttccagtaca cgttcggcat ccagatcgac tacgtcgtct
     1381 acatccgctt caccggattc gagcggttgg tcggcaatgt cagtggcgtg ccggtcaatg
     1441 tcgagaagac gatctacgac aagaagatca ccgatgaccg ctaccccaac aaccagcgcg
     1501 gtgccaagtt cctggcgcag gtgacaactg agctggggag cagcgccccg gcgtgctaca
     1561 cggtcggctc gccggtcaac tggaacgcct caccgaactg cacccgggcg ctcgaatacg
     1621 tgcgcagccg tcacgggccg ggcaacaacg actgggtgcg cggcaggcgc cagcagagct
     1681 tcatcttcgc ggcgatcaag cgcgtcatca gccgcggtag cggcgccaat ttcaattcgc
     1741 tccgcacatc cgcgctgagc aactcgaccg acttctacac gaccctcccg acagacaccg
     1801 gcagcgccct cgaattgttc aacctgctcg ccggcgcgac gatgcccaac caggccgtcc
     1861 tgaagccgac cacctacgcg tcgacggttg cgggaacgtc gaagcagcag ctcaagatcg
     1921 aggtcgtcag gagcctgatg agatcgtggt tcggccccat caactgaccg ggcgggcgac
     1981 gccacccatc tgagcggcca ggcgaccgcc gaacggaggg cgggcagatc ccgggtcggc
     2041 gctgccggaa ccatcaatcg cccgcgcgcc ggtcgcgttg cgcggctgta tcgtgccgcg
     2101 gtgctgatcc gccagttcat cgtcgacggg ctcggtcaca tcagcgcgct cgtggccgac
     2161 gatgcgcgcg gcgtggccgc ggtcattgac ccgcggcgcg atgtcgacgt ctatctcgcc
     2221 gcggccgctg aacggggctg gcgcatcaac cacgtgctcg agacccacct ccacaacgac
     2281 tacatcagcg gtgggccgga actcgcggcg ctgaccggcg cgcgccacgt gatcggcgcg
     2341 ggcgcggaga cggcatacga gcaccaaccg acgcgcgatg gcgacgcgat cgatattggc
     2401 tcgctcaggt tcacgactgt ggatacgcct ggccacacgc ccgagcacgt cgcgtacacg
     2461 ctggccgacc gcggccgcgc cgacgatcca ctggctgtgt tcacgggtgg ctcgctgttg
     2521 gtcggggcgg tcggccggac cgatctgctc ggtgcggaca atgccgaaca gttcgcgcgg
     2581 cagatgttcc actcgctgca cgacaagctc ctcgcgcaca acgattacgt gggcgtgctg
     2641 ccgacccacg gcggtggctc gctgtgctcg aaggacatct cgtccacgcc cagctcgagc
     2701 atcggatttg agcggcgcca cagcgagctg ctggccatcc ccgccatcga ggatttcgtg
     2761 cgggcgctcc tgttcgacca gccgggctac ccgcgctact tcgcgcggat gcggcccatc
     2821 aacaaggccg gcgccacgcc actgggcacg atcccaggtc cacgcgaact gaccgtggcg
     2881 caggtccagg cgctgcttgc cgacgatggg ctagtgatcg atgcgcggcc gtatcccgac
     2941 tatgccgccg gccacctgcc cggctcattc gccattcccg cgggctcgtc cttcggcacc
     3001 tggctgggct gggtggtgcc ggatggccgg ccgttggtgt tcgtgctcga atcggcggcc
     3061 gattgggatg acctgttgcg ccaggcgctg cgcatcggct acgacgacgt gcgcggccat
     3121 ctatcgtcgg ttgccgactg gcgcgcggct ggcctgccgc tcgagaccgg caagcgggcc
     3181 aacatcgagg aactggccac gctgctgaac aggggtggcc cggttgcgcc gctcgtcctg
     3241 gatgtgcgcc agcacaacga gtgggtcgag ggccacatcc cgggcagcgt ccacctgatg
     3301 ggcggcgacc tgcccgatcg tctcgcggag ctgccgcgcg accggccgat catgaccatc
     3361 tgccggaccg ccaaccggag tgccatagcg gccggggtgc tgctgcgcgc cggcttcagc
     3421 gacgtcacct ggatcgacag cggcgtgccg gcctggcgtc gcgcgggctt cccgattgag
     3481 aagggcgagt agtccgcggt agccgcaccg cgagcgcgca gcgggggcgg ccgctcggac
     3541 cagcatccgg gcgagcaggt cataatggcc cgaccgccca gggggttgcc atgcgccgtg
     3601 tgaacaagtc gtcgttcgtc cttgtcctcg ccttctcgtt tgccggcggg ctgcctgccg
     3661 cgcggcccgt cgacgccgca tccgtcagca gcctgaacct gcgcgccacg tacgaggtga
     3721 gcgcgagctt ctcgtgggcg acgcgcgcgg tgagcgtcca tacgactgct cacgtgacaa
     3781 accccacggg cagcgcagtc tcgaccgtcg ccttcaacct ggggacgctg catacgggca
     3841 acgccaacgt gggcgtagtc acggtgcgcg gcagcgcggt cagcgagacg atcaaggatc
     3901 agaccgtgct cgtgcccttc agcccgccgc ttggtgccgg cagcagcgcc gacgtggtca
     3961 tcaactacaa cgcgaggctc gcgtcaaagg cgggcggcta caagtggcag ttcgtccggc
     4021 gcgccggcgt gatgaccgcg taccgctggg tcccgtggct gtcgcgcacg gcacgctttg
     4081 cgaacgcggg cggcggtgag gcgtatgtca cgccatcgag cgcgagcgtg gaggtggcga
     4141 tcacgaccga tcagccgctg gtcatcgcca gcagcggcca gcgcattgcc gtcaacgggc
     4201 tgacccagac cttcgtggcc cacgacgtgc gcgacttcaa cttcagcgcc gcgcccgact
     4261 accagaccgc gacgcgcgtt acccacggca cgacgatcaa gttcttctac cgcacactgc
     4321 cgccttcgac cgtgctcgac aaggcggtcg cgtccttcaa tcacctcagt gacaacgtcg
     4381 gcgcgtaccc cgaggacttc ctcaacatcg ccgaggtcgg gccgtggtac ggcatggaat
     4441 cgcccaatct caactggatc ccgagcgacg ccggcggact gctcaattac atggtggcgc
     4501 acgaggtcgc gcacgaatgg ttctactcca cggtcggcaa cgaccaggcc ctggagccgt
     4561 tcgcggacga ggccctgtcc gagttcctgg ggcgcgagat agcgggcacg tggacatcga
     4621 gcaagtgccc caacgggagg ctcgatagga ccatctacac gacgcctggc gactgctacg
     4681 tcgcggtcgt gtactaccag ggctacaggt acctggatgc ctaccgcgac cacgtcggta
     4741 attcggactt ctaccagggc ctgcgcaact actaccaggc caacaagttc ggcatggggg
     4801 gcacgcgcaa gctgctcaac gccctcgacg ccgcgtcggg ctaccacccc aatcacgccg
     4861 cgcgtttccc gaccctgtat ccctagatcc gcgcgtgagc cccgcgcgcc gctcggcacc
     4921 gccacgtccc aagcgcatcg acgttccaac gcggacggag gccgactcgc aggcgccggc
     4981 gctcagcgag gagatcacgc gcgcctggga cgacccgccc tacgaggagc ggccgcgcgc
     5041 ctcgcgcggc gacgatgtgc cgctgctcgg caatgacgag ctggcgcgca tcttctacga
     5101 gatcggcgac atgctcgaga tccagggcga ggtggtgttc aagacgggcg cctaccgccg
     5161 cgcggcggac aacatcgccc acgcgtcggt cgacgtggcg cgcgcctatc gcgcgggcaa
     5221 tccaccgcgc ctggcgggcg tcggcaaggc cattgacgag aagctcgctg agctcgccga
     5281 cacgggccgg ctgcgctact acgagcgcct gcgccaggac gtgccgccat cggtggtgac
     5341 gttgctccag gtgcccggtc tgggcccgcg caccgcgggc gagctgtggc gccaggccaa
     5401 catctcgacg ctcgaccagc tcgaggccgc ggccagcgcg ggcacgctgc gcgagctcaa
     5461 gggcatgacc gcgcgcaccg aggagcggct gctcacggcc ctgaaa
//
LOCUS       DATBRX010000071         8895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1586113, whole genome shotgun sequence.
ACCESSION   DATBRX010000071 DATBRX010000000
VERSION     DATBRX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586113"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VIF08_02470"
     CDS             complement(<1..186)
                     /locus_tag="VIF08_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY8134877.1"
                     /translation="MISTGDLKKGVVIQLDGEIWQILDYHHIKMGRGSAQVKIKLRNV
                     KRGQTVERSFQAGDKWPR"
     gene            complement(196..1398)
                     /locus_tag="VIF08_02475"
     CDS             complement(196..1398)
                     /locus_tag="VIF08_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY8134878.1"
                     /translation="MTIVAGRLPTAADEARWRSADTAARPRRLELVRERMAREGVGAY
                     FGVRRENIRYLTGLEFSEGEEKGAGNSGQFLVSSDDLVVLADSRYWIQVGEQCPAARM
                     EKVYNDFFDQWPRLLAGIGSPKRVAVEANFVSKLTWDRMAAAAPDVELVPAENWVEAQ
                     RQTKEPDEIERVAAAAAVADAALARIAKTIMAGMTEHEVALALEWAMRTSGAEAIAFD
                     TTVLSGPRAALPHGTPGARQVAAGEVLLFDFGAQVCGYRSDTTRTLFVGEPSSRDLDI
                     YALVLRAQQSAIDALTAALAAGGPLPSGRALHIVARDVIVDGGHGDHFGHGLGHGIGL
                     ATHELPNLGMSGADTPLPSPTVFTVEPGVYLEGETGVRIEDTMVLDAAAGRFEHLSLF
                     PREVTVVG"
     gene            complement(1395..2828)
                     /locus_tag="VIF08_02480"
     CDS             complement(1395..2828)
                     /locus_tag="VIF08_02480"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY8134879.1"
                     /translation="MTFQPRRGTTPRRDPAEVEAQVRSRDRNRSAASRERVAATIERS
                     VAAQQARAASRPRKRGSWRKRLIVGTLLITLLVVGVIVLPPLSGNVFRTLADANPDLM
                     RIGPIADAVGATIGTRPDTPAGTDPTVVEFTIAEGASATDITNDLVARGLVTDRVAFM
                     WLLISGGAINHLEAGVHELARTMTPREVALQLVVPGTPLGGTGVAVLLREGLRIEQIA
                     AYLQTLPFTNFDPDQFYVLATNPPGSLRQKFPWLSVVPQGRSLEGFLGSGLFDIDKTI
                     DAQGMLELLLQAFQDSPSYPLIAQAQAEGKDFYAAVILASIVDHEASLHEEMPLIAGV
                     YQNRLDGLLPTRLLNADPTIIYAKDTMLLRDLHVSDWPDYEFWTLQDIDGVGSFKPTD
                     DLASFQTYHSRGLPDWPICSPGYDALAAALNPDTADGYLYFVAKGDGTRTHAFARTYA
                     EHQANIRYYWGGGATPDPTPTAGVPTP"
     gene            complement(2825..3274)
                     /gene="ruvX"
                     /locus_tag="VIF08_02485"
     CDS             complement(2825..3274)
                     /gene="ruvX"
                     /locus_tag="VIF08_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY8134880.1"
                     /translation="MSRLLALDIGERRIGIALADTESGSVRPLATIRRADAARDAATL
                     ARLATEQSATELVLGLPLNADGSEGTQAQLTREWAASVAAAVALPVTWRDERLTSERA
                     EAIAGRPARGRSGGPPSAKARNAYRSRIDQLAAAGIAQAELDARRGR"
     gene            complement(3271..5055)
                     /locus_tag="VIF08_02490"
     CDS             complement(3271..5055)
                     /locus_tag="VIF08_02490"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEY8134881.1"
                     /translation="MSTIYLEADDEITTAIARLRAVTDGEVVLVVPPGSRVATSRINF
                     KLLAREANERKLNVAAVSDDPAVRALAVSAGLPTYDTIGAAENALANFREQDRRLAER
                     LSGRAGEAKVSDEEAEALRTSRPPLWSSRRGSEMRDSDVRDSDTSIMPAPALDAPLAE
                     QAADNARNATFAAGRARMGATDSRFGDTRAMDTDQRSDIEARSQRRRRGRRIPIAPLL
                     VVGLLALLIGGVAYGAYVLLPTATITLTPQTTTMRLDPFAVTADPNTAVVDPVSGTLP
                     AQNIAVPLHVSDTFNATGTQVSETKATGTVRFRSENTVTPVTVPAGTFVATEDGVEFV
                     TLEAAVVPTADFATSTPGTVNVPVRAVRAGPNGNVDEHTIVLAPRSISSQLVSVSNPA
                     PTSGGARNEASVITRADYDEAVVTLTSRLDHALAVALADPNSVPRGLTAYPATATHDA
                     PTVDQPAEELVGLVAPTFELALDASGHVVAVNEAVIDDIAAARLEAALGPSQQVIGPP
                     PVITVGPGEVADGLITYEVDASVLTYTAPDQPSLVNQVRGKAIAEAETILSAYGIVDI
                     VIWPEFIDRLPDQASRISLTVTPPAAGS"
     gene            complement(5148..6410)
                     /locus_tag="VIF08_02495"
     CDS             complement(5148..6410)
                     /locus_tag="VIF08_02495"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEY8134882.1"
                     /translation="MAFWRRFLKGSDSNKLAACTALDVGTEFAKALVFEINADGTATV
                     HGVGRKRQGLSHMQSGTVADINAVVDNCQVALQEAEEMAGFRSSQVIVGIAGELVKGF
                     TTSMSQERKKPDSQLSARELDVLIDNTQREALKEAERTITWETGLPNVDIRLVHAAVT
                     GATIDGYQVTNPQGFHGRHVRISIFNAFAPLIHLGALQTVAGMLDLELVAIVAEPYAV
                     ARCMDDEQVQQTGALFIDVGGGTTDVALVRQGGIEGTRMFALGGRAFTKSLADRLELP
                     FARAEEIKVDYSRGMTVERHEEVAQIVAEDVAVWQAGVELVLEEFAKTGMLPGRIYLC
                     GGGARLPQITASLRDANFAKALPFARPPQVEAISVKDVAQITDATGLLVDEQDIPPMS
                     LAHQALEMAAAEAPLDEALRRVLRQMKV"
     gene            complement(6415..>8895)
                     /gene="alaS"
                     /locus_tag="VIF08_02500"
     CDS             complement(6415..>8895)
                     /gene="alaS"
                     /locus_tag="VIF08_02500"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY8134883.1"
                     /translation="FFEMLGNWSFGDYFKKEAIAWAIELLTTVYRIPFERLGATTYKD
                     DEEARTYWREMGMPSERMAIWGDVDKGDDSNFWRMADTGPCGPCSEIHFDRGAHLSEG
                     PHCTPDHDENCPRWLEIWNLVFMEFDQPAEGPRVPLPFKSVDTGMGFERLASVLQGVN
                     TNYDTDIFTPIHEKMRSLLGHDPERFEQERFSYQVIADHIRALTFLVADGIRPSNEGR
                     GYVFRRILRRAVRHGRLLGRTEPFLAELSGVVVDSMQGAYPLLTDARPDIYSVIAAEE
                     RQFARTLDAGVVHFEEALIPITGATRKVGGVRPEDLPADSPVLPGDVAFRLHDTYGFP
                     IDLTVELAAEYGVRVDRAGFEAALDEQRQRSRGGKKAELAGHAEMQALYGQLSERTGA
                     TRFLGYETTDAPGKVVAILRDGTDYQELEAKPEAELRLPAEAEAEIVLDQTPFYAEGG
                     GQVGDQGVLKSESGEVIFTVTDTQRPVAGLIVHRGTLHGRVKVGETLTAVVDGVRRAR
                     TMRNHTATHVLHRALRNTVGERARQAGSLVTPDYLRFDFPLDRPLSDDEKRAIETEVR
                     GVVRDNRTVTPKFMTMAEAQAAGADAFFDEKYGEQVRVMFVDGYSRELCGGTHCAATG
                     QIGSFVITGERSIGSGMRRIEALTGDGADAYFNNRLALLDRAAEAAGAKTMDQLPARI
                     AEMKSRGGRGGASPGSDSATESPSALARSAAEVDGTRFLSYAAQFDTMKELQSYAKAL
                     RAELGAGVIALGLEADEPQLFVTVSDDLVSRGVSAGTLVGHGAPLIDGKGGGRPEMAQ
                     ARGTRRDRLPSALTAIREALEATLRNGA"
BASE COUNT         1283 a         3044 c         3078 g         1490 t
ORIGIN      
        1 gcgcggccat ttgtcgccgg cctggaacga acgctccacg gtctggccgc gcttaacgtt
       61 gcgcagcttg atcttcacct gtgccgagcc gcggcccatc ttgatgtggt ggtagtcgag
      121 gatctgccag atctcgccat ccagctggat gacgacaccc ttcttgaggt cgcccgtcga
      181 aatcacggcg gaatgctagc cgacgacggt cacttccctt ggaaacaggc tcaggtgctc
      241 gaagcggccg gccgcggcgt cgaggaccat cgtgtcctcg atgcgcacgc ccgtctcacc
      301 ctcgagatag acgcccggct cgaccgtgaa gacggttggt gacggcaggg gcgtgtcggc
      361 gccgctcatg cccagattgg gcagctcgtg cgtggccagg ccaatgccgt ggcccaggcc
      421 gtgcccgaag tggtcgccgt ggccgccatc gacgatcacg tcgcgggcca cgatgtgcag
      481 cgcacgtccg gacgggagcg gtccgccggc ggccagcgcg gccgtcagcg catcgattgc
      541 cgattgctgg gcacgcaaga cgagcgcgta gatgtcgagg tcgcgcgacg atggctcgcc
      601 cacgaacagg gtgcgcgtcg tatcactgcg gtaaccgcac acctgcgcgc cgaagtcgaa
      661 gagcagcacc tcgcctgccg cgacctggcg cgcgccgggc gtgccgtgtg gcagcgccgc
      721 gcgcggccct gacagaaccg tcgtatcaaa cgcaattgcc tccgcgcccg acgtgcgcat
      781 cgcccactcg agtgcgagtg cgacctcgtg ctcggtcatt ccagccatga tcgtcttcgc
      841 gatccgcgcc agggccgcgt ccgcgaccgc ggcagcggca gcgacgcgct cgatctcgtc
      901 aggctccttc gtctggcgct gcgcctccac ccagttctcg gcaggcacta gctcaacgtc
      961 gggcgcggcc gcagccatgc gatcccaggt gagcttggac acgaagttgg cctcgaccgc
     1021 cacgcgcttg ggcgagccga tgcctgcaag caaccgcggc cactggtcga agaagtcgtt
     1081 gtagaccttc tccatccgcg cggcggggca ctgctctccg acctggatcc agtagcgcga
     1141 gtcggccagt accaccaggt cgtccgagga caccaggaat tggcccgagt tgccggcgcc
     1201 cttctcctcg ccctcgctga actcgagccc ggtcaggtag cggatgttct cgcgccgcac
     1261 accgaaataa gcgccgacac cttcgcgcgc catgcgctcg cgcaccagct ccagccggcg
     1321 tggccgggca gctgtgtcgg ccgatcgcca gcgcgcttcg tcggctgcgg tcgggagcct
     1381 tccggcaacg atggtcacgg tgttggcact cctgctgtag gcgtcgggtc gggggttgca
     1441 ccgccacccc agtagtagcg gatgttggcc tggtgctcgg catacgtgcg cgcgaacgcg
     1501 tgcgtccggg tgccgtcgcc cttggccacg aagtaaaggt agccgtcggc tgtgtccggg
     1561 ttcagggccg ctgcgagcgc gtcgtagccc ggcgagcaga tgggccagtc gggcaggccg
     1621 cgcgagtggt aggtctggaa agaggccagg tcgtcggtcg gtttgaaact gcccacgccg
     1681 tcgatgtcct gcagagtcca gaactcgtaa tcgggccagt cgctaacgtg caggtcgcgc
     1741 agcagcatcg tgtccttggc gtagatgatc gtcggatcgg cgttgagcag gcgtgtgggc
     1801 agcagcccgt cgagtcggtt ctggtagacg ccggcgatga gcggcatctc ctcgtggagc
     1861 gacgcctcgt ggtcgacgat gctcgccagg atcaccgccg cgtagaagtc cttgccctct
     1921 gcctgtgcct gggcgatcag cggatacgag gggctgtcct ggaacgcctg caacaggagt
     1981 tcaagcatgc cctgcgcgtc gatggtcttg tcgatatcaa aaagacccga tcccaggaag
     2041 ccctcgaggc tccgaccctg gggcacgaca ctcagccacg ggaacttctg gcgcaggctg
     2101 cccgggggat tggtcgcgag cacgtagaac tggtcgggat cgaaattggt gaagggcaat
     2161 gtctgcaggt atgcggcgat ctgctcgatg cgcaggccct cgcgcagcag caccgcgacc
     2221 ccggtgccgc cgagcggcgt gcccggcacg acgagttgga gcgcgacctc gcgcggcgtc
     2281 atcgtccggg cgagctcgtg cacaccagcc tctagatgat tgatggcacc gccacttatc
     2341 agcagccaca tgaaggccac gcgatcggtg acgaggccgc gcgcaacgag gtcgttggtg
     2401 atgtcggtcg cactcgcacc ctcggcgatc gtgaattcca ccacggtcgg gtcggtgcca
     2461 gcgggagtgt ccggccgggt accgatggtc gcgccaaccg cgtcagcgat tggaccgatg
     2521 cgcatcaggt cggggttggc gtcggccagg gtccggaaca cgttgcccga cagcggcggc
     2581 aggacgatca cgccgaccac gagcagagtg atcagcagcg tgccaacgat gaggcgctta
     2641 cgccatgagc cgcgcttcct tggtcggctg gcggcgcgcg cctgctgcgc ggcgacgctc
     2701 cgttcgatgg tcgcggccac gcgttcgcga ctggccgcgc tgcggttgcg gtcgcggctg
     2761 cgaacctgcg cctcgacctc cgctggatcg cgccgcggag tggtgccgcg gcgtggctgg
     2821 aaggtcatct gccgcgccgc gcgtcgagct cggcctgggc gatgccagcc gcggcaagct
     2881 gatctattcg cgaccggtag gcgttgcgcg ccttggcgga tggcggtccg ccggagcgac
     2941 cgcgcgctgg ccggccggca atggcttcgg cgcgttcgct ggtcaggcgc tcgtcgcgcc
     3001 aggtgacggg caacgcaacg gcggcggcca cggaagccgc ccactcccgc gtcagctgcg
     3061 cctgcgtgcc ttcgctgccg tccgcgttga gcggcaggcc aaggacgagc tcggtcgcgc
     3121 tctgctcagt ggcaaggcgg gcaagcgtgg ccgcgtcgcg cgccgcatcg gcgcgccgaa
     3181 tcgtggccag gggcctgacc gagccgctct ccgtatcggc gagcgcgatg ccgatgcggc
     3241 gctccccgat gtccagggcg agcagccggc tcacgaacct gccgcaggcg gcgtcaccgt
     3301 caggctgatg cgcgacgcct ggtcgggcag ccggtcgatg aattccggcc agatgacgat
     3361 gtccacgatc ccatatgcgg aaaggatggt ctctgcctcc gcgatggcct tgccgcgtac
     3421 ctggttgacg agcgacggct ggtcgggcgc ggtgtaggtc aggacagacg cgtcgacctc
     3481 gtaggtgatg aggccgtccg cgacctcgcc tggaccaaca gtgatgaccg gcggcggacc
     3541 gatcacctgc tgcgagggtc cgagtgccgc ctcgagccgc gcggcggcga tgtcgtcgat
     3601 caccgcctcg ttgaccgcga ctacgtggcc cgacgcatcg agcgcgagct cgaaggtggg
     3661 cgcgacgagg cccacgagct cctcggcggg ctgatctacg gtcggcgcgt cgtgggtcgc
     3721 ggtggcggga tacgcggtca ggccacgtgg caccgagttc gggtccgcga gggcgacggc
     3781 cagcgcgtgg tccagccgtg acgtcagcgt gacgaccgcc tcgtcgtaat cggcccgcgt
     3841 gatcacgctc gcctcattgc gcgcgccacc gctggttggc gccgggttgc tgaccgacac
     3901 gagctgcgat gagatcgagc gcggcgccag gacgatcgtg tgctcgtcga cattgccgtt
     3961 cggcccggct ctgacggccc gcaccgggac gttgaccgtg cccggggtgg acgtcgcgaa
     4021 gtcggccgtc ggcaccacgg cggcctcgag cgtgacgaac tcgacgccgt cctcggtcgc
     4081 cacgaaggtg cccgccggaa ctgtgaccgg ggtgaccgtg ttctcgctgc ggaatcggac
     4141 ggttccggtg gccttggtct cgctgacctg ggtgcccgtc gcattgaacg tgtcgctcac
     4201 gtgcagcggc acggcgatat tttgcgccgg cagcgtgccg ctcacgggat cgacgacggc
     4261 cgtgtttgga tcggccgtga ccgcaaaagg gtcgaggcgc atggtcgtgg tctggggcgt
     4321 gagggtgatg gtcgcggtcg gcagcagcac ataggcgccg taagccacgc cgccgatcag
     4381 cagcgcgagc agtcccacca ccaggagcgg cgcgatgggg atccgccggc cgcgccgccg
     4441 acgttggctg cgcgcctcga tatcgctccg ctggtcggta tccatggcgc gcgtgtcgcc
     4501 aaagcggctg tccgtcgcgc ccatgcgcgc acggcccgcg gcgaacgtgg cattgcgcgc
     4561 gttgtcggcg gcctgctcgg cgaggggcgc gtccagggct ggcgcgggca tgatgctcgt
     4621 gtcgctgtcg cgcacgtcgc tgtcgcgcat ttcgcttccg cgcctcgatg accacagtgg
     4681 tggccgtgat gtgcggagcg cctccgcctc ctcgtcgctc accttggctt cgcccgcgcg
     4741 gccgctcagg cgctcggcga gcctgcggtc ctgttcgcgg aagttggcca gcgcgttctc
     4801 ggccgcgccg atggtgtcgt aggtgggcaa cccggcggaa acggccagcg cccgcactgc
     4861 cggatcgtca ctgactgcgg cgacgttgag cttgcgctcg ttggcctcac gcgcgagcag
     4921 cttgaagttg atgcgcgacg tggcgacgcg cgagccgggt ggcacgacca gcacgacctc
     4981 gccgtccgtg accgcgcgca gccgcgcgat ggcggtcgtg atctcatcgt ctgcttcgag
     5041 gtagatcgtg ctcatcgcaa gccgagacgc aggcccgcgg ctgcggtccg gcgccgagtg
     5101 cgcgcagcag cgcccgtgag ggcgcctgcg gaggttgggt tcatttccta aaccttcatc
     5161 tggcgcagca cgcgccgcag cgcctcatcg agcggcgctt ccgccgcggc catctcgagc
     5221 gcctggtgag cgagcgacat gggcgggatg tcctgctcat cgacgagcag cccggtcgcg
     5281 tcggttatct gggcaacatc cttgacgctg attgcctcca cctggggcgg ccgcgcgaaa
     5341 ggcagtgcct tggcgaagtt cgcgtcgcgc aatgatgcgg tgatctgggg tagccgcgcg
     5401 ccaccgccgc acaggtagat gcggccgggc agcatgcccg tcttggcgaa ctcctcgagc
     5461 accagctcca ccccggcctg ccataccgcc acgtcctccg cgacgatctg cgcgacctct
     5521 tcgtggcgct caacggtcat gccgcgcgaa tagtcgacct tgatctcctc tgcccgtgca
     5581 aagggcagct cgagcctgtc ggcgagcgac ttggtaaatg cgcgcccgcc cagcgcgaac
     5641 atgcgcgtgc cctcgatgcc gccctggcga acgagcgcca cgtctgtcgt tccgccgccg
     5701 acgtcgatga acagcgcgcc ggtctgctgg acctgctcgt catccatgca tcgcgcgacc
     5761 gcgtagggct cggccacgat cgcgacgagc tccaggtcga gcatgccagc gactgtctga
     5821 agcgcaccga ggtggatcag cggcgcgaac gcgttgaaga tgctgatgcg cacgtggcgg
     5881 ccgtggaagc cctgcgggtt cgtgacctgg tagccgtcga tcgtggcccc ggtgaccgcg
     5941 gcgtggacga gccggatgtc gacgttgggc aggccggtct cccaggttat ggtccgctcc
     6001 gcctccttga gcgcctcgcg ctgggtgttg tcgatgagta cgtccagctc gcgcgccgac
     6061 agctgcgaat cgggcttctt ccgctcctgc gacatgctcg tggtgaagcc cttcacgagc
     6121 tcgcccgcta tgccgacgat gacctggctc gaacggaagc cggccatctc ctccgcctcc
     6181 tggagggcga cctggcagtt gtcgaccacg gcgttgatgt cggcgactgt gcccgactgc
     6241 atgtgcgaaa gtccttgccg tttccggccg accccgtgca ctgttgccgt cccgtcggcg
     6301 ttgatctcga acaccagcgc tttcgcgaac tcggtgccga catcgagcgc ggtgcacgcc
     6361 gcgagcttgt tgctgtcgct gcccttgaga aatcgccgcc agaaggccac tcgactacgc
     6421 cccgttgcgc agcgtcgctt cgagtgcctc gcggatcgcc gtcagggcgc tgggcagccg
     6481 atcacgccgc gtgccgcggg cctgcgccat ctccggcctg ccgccgccct tgccgtcaat
     6541 caggggcgcg ccatggccca cgagcgtgcc ggcgctgacg ccgcgcgaaa ccaggtcgtc
     6601 gctcaccgtg acgaagagct gcggctcatc cgcctccagc ccgagcgcga tcacgcctgc
     6661 gcccagctcg gcgcgcagtg ccttggcgta cgactgcagt tccttcatcg tgtcaaattg
     6721 cgcggcgtat gagagaaagc gcgtcccgtc gacctccgcg gcggacctgg cgagcgccga
     6781 tggcgactct gtagcgctgt cgctgcccgg gctcgcaccg ccgcgcccgc cgcgcgactt
     6841 catctcagcg atgcgcgccg gcagctggtc catcgtcttg gcgccggccg cttccgccgc
     6901 gcggtcaagg agtgccagac gattgttgaa gtacgcgtcc gcaccgtcgc cggtcaacgc
     6961 ctcgatccgg cgcatgccgc tgccgatcga tcgctcgccg gtgatcacga acgagccgat
     7021 ctggccggtc gcggcgcaat gggtgccgcc gcacagctcg cgcgagtagc cgtcgacgaa
     7081 catgacgcgc acctgctcgc cgtacttctc gtcgaagaac gcgtcggcgc ccgcggcctg
     7141 cgcctcggcc atggtcatga acttgggcgt cacggtgcgg ttgtcgcgca cgacgccgcg
     7201 cacctcggtc tcgatggcgc gcttctcgtc atcgctgagc ggccggtcga gcgggaagtc
     7261 gaagcgcagg tagtcgggcg tgaccagcga cccggcctgg cgggcgcgct cgccgaccgt
     7321 gttgcgcaac gcacgatgca gcacgtgcgt ggcggtgtgg ttgcgcatcg tcctggcccg
     7381 ccgcactccg tcaacaacag cggtcagcgt ctcccccacc ttgacgcggc cgtgcagcgt
     7441 gccgcggtgc acgatcaggc ccgcgaccgg gcgctgcgtg tccgtcacgg tgaagatcac
     7501 ctcgcccgat tccgacttca ggacgccctg atcaccgacc tggccaccac cctccgcgta
     7561 gaagggtgtc tggtcgagca cgatctccgc ctcggcctcg gccggtagtc ggagctcagc
     7621 ttccggcttg gcctccagct cctggtagtc ggtgccatcg cgcaggatcg cgaccacctt
     7681 gcccggcgca tccgtggtct cgtagcccag gaacctggtc gcgccggtgc gttcgctcag
     7741 ctgtccgtac agggcctgca tctccgcgtg cccggcgagc tccgccttct tgccgccgcg
     7801 cgagcgctgg cgctgttcgt cgagcgcggc ctcgaagcct gcgcggtcga ctcttactcc
     7861 gtattccgcc gcgagctcga cggtcaggtc gatcgggaag ccgtacgtgt catgcaggcg
     7921 gaacgcgaca tcaccgggca ggaccggtga atcggccggc aggtcttccg ggcgcacgcc
     7981 gcccaccttg cgcgtcgcgc cggtgatggg gatgagtgcc tcttcgaaat gcaccacgcc
     8041 cgcgtccaac gtgcgagcga actggcgctc ctccgcggcg atgacgctgt agatatccgg
     8101 gcgcgcgtcg gtcagcagcg ggtaggcacc ctgcatggag tcgacgacga cgccggacaa
     8161 ctcggccagg aacggctccg tgcggccgag cagccggccg tggcgcaccg cgcgtcggag
     8221 gatgcgccgg aagacgtagc cgcggccctc gttcgagggg cggatgccgt ccgccacgag
     8281 gaaggtcagc gcccgtatgt ggtcggcgat gacctggtag ctgaaacgct cctgctcgaa
     8341 cctctccgga tcgtggccca ggagcgaacg catcttctcg tggatgggcg tgaagatgtc
     8401 ggtgtcgtag ttggtgttga cgccctgcag gacgctcgcc aggcgctcga agcccatgcc
     8461 tgtgtcgacg ctcttgaacg gcagggggac acgcggcccc tcggccggct ggtcgaactc
     8521 catgaagacg aggttccaga tctccagcca gcgcgggcag ttctcgtcgt gatcgggggt
     8581 gcagtgcggc ccctccgata ggtgcgcccc gcggtcgaag tggatctcgc tgcacggacc
     8641 gcacggcccg gtgtcagcca tgcgccagaa gttgctgtcg tcgcccttgt cgacgtcgcc
     8701 ccagatggcc atgcgctcgg atggcatgcc catctcgcgc cagtacgttc gcgcctcttc
     8761 gtcgtccttg taggttgtcg caccgaggcg ctcgaacggg atcctgtaga ccgtcgtcag
     8821 cagctcgatc gcccaggcaa tcgcttcctt cttgaagtag tcgccgaacg accagttgcc
     8881 gagcatctcg aagaa
//
LOCUS       DATBRX010000072         8085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2591293, whole genome shotgun sequence.
ACCESSION   DATBRX010000072 DATBRX010000000
VERSION     DATBRX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591293"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VIF08_02505"
     CDS             complement(<1..422)
                     /locus_tag="VIF08_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8134884.1"
                     /translation="MTILFPPPISPSLIIGLAALLLGVLGFAIAARLRGSTLGSLVVT
                     LRLLFLAVGVVMTLSGLFAATPQTQLANPIPATVDSISAGEEVYLNTCAACHGVDARG
                     GGPQSGTTQVRPPALVGPGSHLGQHTDGDLHYFIGNGLP"
     gene            complement(436..2277)
                     /locus_tag="VIF08_02510"
     CDS             complement(436..2277)
                     /locus_tag="VIF08_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEY8134885.1"
                     /translation="MRRLKAALLAVALFWIAGPTLAGPAFQAVSAHAQLVSSVPGAGE
                     RLDQAPSQIILVFSERLDQLGTAVDLLDADGRLILRGSGAIDPADQRSFLVPLPALGD
                     GLYTVNWRSLSADDGHSIQGFFSFGIGDDVVVPGAGQGDGGDIHSGHGLVQSVIETIA
                     RIAAELGAMLALGFVLIALAVLRPVDAGAARVVGRWASLALIAAGLGAAALIPLVAST
                     AVVDPVAYALQTGSGQLLVARAALGLGIGSVALLIARGAELSAAFAAAGGVALTLLLA
                     LSGHGAGFESAAPILFVAVHVAAAGCWLAGLLGLAWLILGNGGRHRPLLGASIPRFSA
                     VALVSVGLFATTSLYLWWLMNRAVIDLASNYGVLLVVKAVLALAAIALGGLSYLGWRS
                     TGALGVNRRIPIEAGLALAVVAVTALLASGSPPGPTRPIPIERAATSSPNELNANLSL
                     LPARPGPNRVIVNLPDSVVTGHETLELVLQRLDQTGETRVVLTLVPDNPQIGATGATD
                     VPMPPGSQWDASARLMLHDAELSRARFVFAFDDAGLSQGQSRPVLDPLALVALALAAA
                     AVFGFTIVLAGGALPRVERRLGRLTLLAGSIAAALLAVAAFIVGPQL"
     gene            complement(2297..2899)
                     /locus_tag="VIF08_02515"
     CDS             complement(2297..2899)
                     /locus_tag="VIF08_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134886.1"
                     /translation="MSISLLRRGLTMAAVLILLALTAITPVLAHEERTVAGYDMEVGM
                     IGEPVFTGQKSGLEFSVFKDDVPVIGLQDTLQAEAIFGEAHRNLPISRRFGEDGWYQS
                     YFFPTVAGPYTFRIFGTIEGNAIDETFTAGPDGFGEVQEASSGQFPTTLPALSDISAQ
                     AQKGADAAGQVTLALGLGGLGFLLGLVGIGLAVAARRRPA"
     gene            complement(2964..3635)
                     /locus_tag="VIF08_02520"
     CDS             complement(2964..3635)
                     /locus_tag="VIF08_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134887.1"
                     /translation="MVLHATPVQVRRWRRLLYLALLVVALAVTHEAVFIVRGHDATGI
                     FPTDPAHAYWPAFLVMALVAFAGLAWWARWRLTSGVRSAAANPTTADRPADGTFDQWQ
                     RYFARLAPTFAAAFLLLENAEHLMSHGHIEGVGVYTAPGAELALPVLLSVVGAISLLG
                     ALVRWREITLIERLRAMRARVAHRRHGPISQAPHWRVTAALVQLELLMARDDHGRAPP
                     QASAI"
     gene            3769..4017
                     /locus_tag="VIF08_02525"
     CDS             3769..4017
                     /locus_tag="VIF08_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY8134888.1"
                     /translation="MPVNVHEAKTHLSRLLARVEAGESIVIARAGRPIAVLAPVDGPT
                     SRKPGNEKIVIGPDFDDPLPEFDPEYAHPDDPLLNAKP"
     gene            4014..4403
                     /locus_tag="VIF08_02530"
     CDS             4014..4403
                     /locus_tag="VIF08_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY8134889.1"
                     /translation="MRLLVDTHALLWWVTSGGSRLSVQARDLLSDSSTDALVGAGSLY
                     EIAYKSSIGRLQLPGRPEAYLPRLLRRHAFAVLPIADVHAIHAGGLPLIHRDPWDRLL
                     VAQAQVESLPILTTDPAIGQYDVDVIW"
     gene            complement(4434..4835)
                     /locus_tag="VIF08_02535"
     CDS             complement(4434..4835)
                     /locus_tag="VIF08_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8134890.1"
                     /translation="MALTVVDAGVVIAVLDAADSHHAASVDAVRRAIASGESLVLPVS
                     AYAEVLVAPFRRNLESAAVVDSFIDALPATVQPITREVARMAAQLRAQHGPRLRLPDA
                     LVVATALELSADRLLTTDKRWPDVGLKLETA"
     gene            complement(4826..5080)
                     /locus_tag="VIF08_02540"
     CDS             complement(4826..5080)
                     /locus_tag="VIF08_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134891.1"
                     /translation="MREVKVGRRRQRGVTRISTQNQVTLPTDALAQAGLAAGDRLRAR
                     VQAPGQILLVRESDPIDQYAGALTGLYPPNYLDELRDEWR"
     gene            5156..6145
                     /locus_tag="VIF08_02545"
     CDS             5156..6145
                     /locus_tag="VIF08_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134892.1"
                     /translation="MSLLRTGVWTAVVLSIGACGAAAPSATGWTYGPEDAARDKLATL
                     IEDVTSADAYRYDPHDDRGHTLTGPKILQVPDGTFVATYFSWLEAGRRFQTHVATSSD
                     LINWTWQRTLGENASQPTIAEASDGGYVVGWEQEPPNHLLFAYYESLDDLLAGNAQKT
                     YEPPTQLSPDCAEGTPSFYSASSTFLDVGFHYYRDCELDRQAHGTTNWVSWTSSPAPD
                     LDAAIERLGVEGGIGDRDGPLVFRGFEFMMFEGMRQLPLWETFRVFLYDRTSGSARQL
                     DMQTHGGSRAFTNPTIGQVTLDGRAVLVVSLFIPGEGSAQDEAGGVIYYRFID"
     gene            6159..7349
                     /locus_tag="VIF08_02550"
     CDS             6159..7349
                     /locus_tag="VIF08_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8134893.1"
                     /translation="MTEPTRATSGEAAPQDVLAALGEVIRAVSSSGFELDDVLQTIIR
                     RAVELSRVDFGSILRPHGDHYQMVAHHGQVSDAYIDLVRRFKYVPDRGSMIGRTLLAK
                     APVQIVDVLADPDYTAWEIQKAGNYRTLLGVPMLRGDEWVGVFVLTRHEVQPFTDQQI
                     SMLSTFADQAVLAIDSLRLVQTIQSQRTELARYAPQAAELMSTESGVALLAGHRREIT
                     AMFCDLRGFTAFAESAEPEEVLGVLREYHSMVGEIVVGAGGTVEHFAGDGLMAFFNDP
                     RALENHQLVAVRSAIAIRDRFAQLAAGWSKRGYELGVGIGLALGFATLGRIGFEGRFD
                     YGAVGNVVILASRLSDAAGPAEILLSQRLNAAVEGSVATDALPDLALKGFSKPVQAFR
                     VTSE"
     gene            7655..7900
                     /locus_tag="VIF08_02555"
     CDS             7655..7900
                     /locus_tag="VIF08_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134894.1"
                     /translation="MGTVRDRDVVSQGSLDAIWAWLKDQHIVIQALVWLFFLPVTAGL
                     WKWETGWPLAGRLVAIGGLALATLFVFFPRFLLGPRP"
     gene            complement(7897..>8085)
                     /locus_tag="VIF08_02560"
     CDS             complement(7897..>8085)
                     /locus_tag="VIF08_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134895.1"
                     /translation="SPRDGVDSVSPGPGVVYSQRLSAQRTKSRLNRIVGTPEYRSMTI
                     RNWNTTAKLLELLRKRAA"
BASE COUNT         1418 a         2676 c         2735 g         1256 t
ORIGIN      
        1 ggcaggccgt tgccgatgaa gtagtgcagg tcgccatctg tgtgttggcc caggtgactg
       61 cccggaccga ccagcgccgg tggtcggacc tgtgtcgtcc ccgactgtgg tccgccgccg
      121 cgagcatcaa ctccgtggca cgcggcgcag gtgttcaggt agacctcctc acccgcgctg
      181 attgaatcga ccgtggccgg aatcgggttg gccagctgcg tctgcggcgt cgccgcgaag
      241 aggcccgaga gggtcatcac cacgcccacg gccaggaaca gcagccgcag cgtcacgacc
      301 aagctgccga gcgtcgagcc gcgcaggcgt gcggcgatgg cgaacccgag cacccccagg
      361 agcagagcag ccagcccgat gatcaggctc gggctgatcg gcggcgggaa gaggatcgtc
      421 accggaagac gtacctcaca gctgtggccc cacgataaat gccgcgaccg ccaggagcgc
      481 ggcggctatg gatccagcca agagcgtcag gcggcccagg cgtcgttcga cccggggcag
      541 tgccccgccg gcgaggacga tggtgaagcc aaagaccgcc gctgctgcga gggccagggc
      601 caccagggcg agcggatcga ggacgggccg cgattggccc tggctgagcc cggcatcgtc
      661 gaacgcgaac acgaagcggg cacgcgacag ctccgcgtcg tgcagcatca gccgggcgct
      721 cgcgtcccac tggctgccgg gcggcatcgg cacatccgtc gcgcccgtgg cgccgatctg
      781 tggattgtcc ggaacaagtg tcagcaccac cctggtttcg ccagtttgat cgagccgctg
      841 aagcacgagc tcgagcgtct cgtggccggt caccacggag tccggcaggt tgacgatcac
      901 gcggttcggt ccgggtcgcg cgggcagcag cgacaggttg gcgttgagct cgttggggct
      961 gctggtcgcg gcgcgctcga tcgggatggg ccgcgtgggg ccgggcggtg agccacttgc
     1021 cagcagcgcg gtcaccgcga ccacggcaag ggccagtccc gcttcgatgg gaatgcggcg
     1081 gttgacgcca agcgcgcccg tggatcgcca accgaggtag ctcagcccgc ccagcgcgat
     1141 ggcggcgagg gcgagcaccg ccttgacgac gagcaggacg ccgtaattcg atgcgaggtc
     1201 gatcacggcg cggttcatca gccaccacag gtacaggctg gtcgtcgcga acaggcccac
     1261 ggacacgagg gcgaccgcag aaaagcgcgg gatcgatgcg ccgagcaacg gtcggtgcct
     1321 gccgccgttg cccaggatca gccacgccaa cccaaggagg ccggccagcc agcagcccgc
     1381 ggccgcgaca tggacggcaa cgaagaggat gggcgcggcg gactcaaacc cggcgccgtg
     1441 gccggacagg gcgagcagca gagtgagtgc cacgccacca gcggcggcga aggcggccga
     1501 gagttccgcg ccgcgcgcga tcagcagtgc cacgctgcca atgcccagtc cgagcgcggc
     1561 tcgggcgacg agaagctgtc cggagccagt ctggagggcg taggccaccg gatcgactac
     1621 tgccgtgctt gccaccagcg gaatgagcgc cgccgcacca agaccggcgg cgatgagcgc
     1681 caacgaagcc caccggccga ctacccgcgc ggcccccgca tcgacgggac ggaggactgc
     1741 aagggcgatc agcacgaagc ccagggcgag catcgcaccc agctccgccg caatgcgtgc
     1801 gatcgtttcg atgaccgact gaacgagacc gtggcccgaa tgaatgtcgc cgccgtcacc
     1861 ctggccggcg cccggcacca cgacgtcgtc gccgatgccg aagctgaaga agccctgtat
     1921 cgaatggccg tcatcggctg acagagagcg ccagttgacc gtgtacaggc cgtcgcccag
     1981 cgccggcagc gggacgagga acgatcgctg atcggccgga tcgatggcgc ccgagccacg
     2041 caggatcagc cgaccatcgg catcgagcag atcaacggcg gtgcccagct gatcaaggcg
     2101 ctcagagaag acgaggatga tctggctcgg cgcctggtcg agccgctcac cggcgccggg
     2161 cacgctgctc acgagctggg cgtgcgcgga caccgcctgg aaggctggcc cggcgagcgt
     2221 cggaccagca atccagaaca aggcgacggc cagcagcgct gccttcagac gtcgcatgag
     2281 gcgaaccgac tccgcgtcag gcaggacgcc tgcgcgcggc aacggccagg ccgatcccga
     2341 ccagcccgag cagaaagccc agtccgccca gccccagtgc gagcgtcacc tggccggccg
     2401 cgtcggcgcc cttctgggcc tgcgctgaga tgtcgctcag cgcggggagg gttgtgggga
     2461 actgaccgga ggatgcctcc tggacctcgc cgaagccgtc ggggccagcg gtgaacgtct
     2521 catcgatcgc gtttccctcg atcgtgccaa agatgcggaa ggtgtacggg ccggcgacgg
     2581 tcgggaagaa gtacgactgg taccagccgt cctcgccgaa acgcctgctg atcggcaggt
     2641 ttcgatgcgc ctcgccgaag atcgcctcag cctggagcgt gtcctgtagg ccgatcaccg
     2701 gcacgtcgtc cttgaacacg gaaaactcga ggccgctctt ctgtcctgtg aagacgggct
     2761 ctccgatcat gccgacttcc atgtcgtagc cggcgacggt ccgctcctcg tgcgcaagaa
     2821 cgggagtgat cgccgttaac gcaagaagaa tgaggaccgc ggccatcgtc agcccgcgcc
     2881 gcagcagaga aatagacacg aaatacctcc gacagtctcg attggcgtgt cctgacggcg
     2941 ccttttcgcg cgcccggacg aagtcagatc gcggaggcct gtggcggtgc cctgccgtgg
     3001 tcgtctcggg ccatcaagag ctcaagctgg acgagcgcgg cggtgacgcg ccagtgcggc
     3061 gcctgcgaaa tggggccgtg tcggcgatgt gccacccggg cacgcatggc gcgcaaccgc
     3121 tcgatgagcg tgatctcgcg ccagcgcacg agggcgccca gcagcgagat ggcgccgacc
     3181 accgacagta gcaccggcag cgcgagttcc gctcccggtg cggtatagac gcccacgcct
     3241 tcgatgtggc cgtggctcat caggtgctcg gcgttctcga gtagcaggaa cgccgccgcg
     3301 aacgttggcg cgaggcgcgc gaagtaccgt tgccattggt cgaaggtgcc gtccgcgggt
     3361 cgatcggccg tcgtggggtt cgcggccgct gagcgaacgc cactggtcag gcgccatcga
     3421 gcccaccagg ctaggcccgc gaacgcgacc agcgccatga ccaggaatgc cggccagtac
     3481 gcgtgcgcag ggtcggtcgg gaaaatgccc gtcgcgtcgt ggccgcgtac gatgaacaca
     3541 gcctcatgcg taaccgcgag cgcgacgacg agaagtgcga ggtacaggag tcgccgccag
     3601 cgacgaacct ggaccggggt cgcgtggaga accacgacgg ccagcctacc tcaaccagga
     3661 gctgcccatg gcctcgcccg cggctagatc actgctcgga gctgggcggc cgcccggtcc
     3721 gtgtttagtc gagttgacta accaagttga ctaaggcaga ctccggccat gccggtaaat
     3781 gtccacgagg ccaagacgca tctatcccgc ctcctggcgc gcgtcgaggc cggcgagagc
     3841 atcgtcattg ctcgggccgg tcggccaatc gcggttctcg ctccggtcga cggaccaacc
     3901 tcacgcaagc ctgggaacga gaagatcgtc atcggaccag acttcgacga tcctcttccc
     3961 gagttcgacc cggaatacgc gcaccccgac gacccgctcc tcaacgccaa gccgtgaggc
     4021 tactggtaga tacgcacgcc ctgctgtggt gggtgacctc gggcggatcg cgcctgagcg
     4081 tccaggccag agacctcctc tccgattcgt cgacggatgc gctcgtgggt gccggctccc
     4141 tgtacgagat cgcgtacaag tcgagcatcg gccggcttca gcttcccggt cggccagagg
     4201 cgtacttgcc gcgtctgctg cgtcgacatg ctttcgcggt gctgccaatc gcggacgttc
     4261 atgccatcca cgcaggcggc ctgccgctga tccatcgcga cccatgggat cggctgctgg
     4321 tcgcccaagc gcaggtcgag agcttgccaa ttctcaccac ggatccggcc ataggtcagt
     4381 acgacgtcga cgtcatctgg tagagcacga cctgttgtcg agatcccacc catttaggcc
     4441 gtctccaatt tgaggccgac gtctggccag cgcttgtccg tcgtcaggag ccgatcggca
     4501 gacagctcga gcgccgttgc cacgacgagc gcgtcaggca accgcaggcg cgggccatgc
     4561 tgcgcgcgga gttgagccgc catgcgggct acctcacgag ttatgggctg cacggtcgcg
     4621 ggcagtgcgt cgatgaatga atcgacgacc gcggcggact ccaggttgcg ccggaagggc
     4681 gcgaccagga cttcggcgta ggccgataca ggcagaacga ggctctcccc acttgcaatc
     4741 gcacgacgca ccgcatcaac ggacgcggcg tgatgggaat ctgccgcgtc caggacggca
     4801 atgacgactc cggcatcaac gactgtcagc gccactcgtc ccgaagctcg tcgaggtaat
     4861 tgggtgggta caggccggtc aaggcgcccg cgtattggtc gatcgggtcc gattcgcgca
     4921 ccaggaggat ctggccgggt gcttgaactc gcgctcgcag gcggtcgccg gcggcaagtc
     4981 cggcctgagc cagtgcgtcc gtgggcagcg tcacctggtt ctgcgtgctg atgcgcgtga
     5041 ctccacgctg cctgcggcga ccgaccttta cttctcgcac tatgagtaaa tattacacca
     5101 cggcgctcac cggagcctta tctcgccctc cacagacgcc gcgtagtctt tcgtcgtgag
     5161 cttgctccgg acaggagtgt ggacagcagt cgtgctgtca atcggtgcgt gcggagcggc
     5221 tgcgccgtcg gcgaccggct ggacgtacgg cccggaagat gcggcgcgcg acaagctcgc
     5281 gacgctgatc gaggacgtca cgtccgccga cgcataccgc tacgacccgc atgatgaccg
     5341 cggccacacc ttgaccgggc cgaagatcct gcaggtcccg gacggcacct ttgtagccac
     5401 gtacttctca tggctggagg caggccggcg attccaaacg cacgtcgcga cgtcgagcga
     5461 cctcatcaac tggacgtggc aacggaccct gggcgagaac gcctcgcagc cgacgatcgc
     5521 ggaggccagc gatggtggct acgtcgtggg ctgggagcag gagcccccga accatctgct
     5581 cttcgcctac tacgaatcgc tcgacgatct gctcgcgggc aacgcgcaga agacgtacga
     5641 gccgcccacc cagctgtcac ccgactgcgc cgaaggaact ccttccttct attcggccag
     5701 cagcacgttc ctggatgtcg gcttccacta ctaccgcgat tgcgagctgg accggcaggc
     5761 acacgggacg accaattggg tcagctggac gtcgagcccc gcgcccgatc tggacgcggc
     5821 gatagagagg ctcggcgtgg agggtgggat cggcgaccgc gatggcccgc tcgtcttccg
     5881 tggcttcgag ttcatgatgt tcgagggcat gcgccaactc ccgttgtggg agacctttcg
     5941 ggtattcctg tacgaccgaa cgagcggcag cgccaggcag ctggacatgc agacgcacgg
     6001 cggcagcagg gcattcacga atcccacgat cggccaggtg actctcgacg gccgcgccgt
     6061 cctcgtcgtg tcgctgttca tccccggcga aggcagcgct caagatgaag cgggcggagt
     6121 tatctactac cgcttcatcg actaactaac ctaggtcagt gacggagccc acccgcgcga
     6181 cttctggcga ggcggcgccg caggacgtgc tggcggcgct gggtgaggtc atccgcgccg
     6241 tttcgagtag cggcttcgag ctcgatgacg tgctgcagac gatcatccgc cgcgccgtcg
     6301 agctgagtcg cgtcgacttt ggcagcattc tgcggccgca tggcgaccac taccagatgg
     6361 tcgctcacca cggccaggtg agcgacgcgt acatcgacct ggtcaggagg ttcaagtacg
     6421 ttcccgaccg cggctcgatg atcggccgga cgttgctcgc caaggcgccg gtgcagatcg
     6481 tggacgtcct cgccgacccc gattacacgg catgggagat ccagaaggcc ggcaactacc
     6541 gcacgctgct cggtgtgccc atgctgcgcg gcgacgagtg ggtcggagtg ttcgtcctga
     6601 cccgccacga ggtgcagcca ttcaccgacc agcagatctc gatgctttcg acttttgccg
     6661 accaagcggt cctggccatc gacagcctcc gactcgtcca gacaatccag tcgcagcgca
     6721 ccgagctggc gcgctatgca cctcaggcgg cggagctgat gtcgaccgaa tccggagtcg
     6781 cactgctcgc cggccaccgg cgcgagatca cggcgatgtt ctgtgacctg cgcggcttca
     6841 ccgccttcgc cgaatcggcg gagccagagg aagtgctggg cgtcctgcgc gagtaccact
     6901 cgatggtcgg ggagatcgtc gtcggcgcgg gcggcacggt cgaacatttc gcgggtgacg
     6961 gcctcatggc gttcttcaac gacccgcgcg cgctcgagaa ccaccaactg gtcgcggtgc
     7021 gcagcgccat cgccatccgt gaccgatttg cgcagctggc cgcgggctgg agcaagcgcg
     7081 gctatgagct gggcgtaggc atcgggctgg ccctgggctt tgcgaccctg ggtcgaatcg
     7141 ggttcgaagg acgcttcgac tacggcgccg tgggcaacgt ggtcatcctc gcctcgcgcc
     7201 tgtccgacgc ggccggtccg gcggagatcc tgctcagtca gcgcctcaac gccgcggtcg
     7261 agggctcagt ggcaacagac gccctgcccg atctggccct gaagggcttc agcaagcccg
     7321 tccaggcgtt ccgcgtcacc agcgagtagg cgctgccagc cggcctctag tagccgctgc
     7381 gcggatctcg ccggttgccg cggatgattc cccagagctt gcggattgcc cagagtcgga
     7441 aaagcaaact gatcatggtg tcctccctgc aggccaccga ttcaaccgcc gcggccgacc
     7501 aaaccgccct cgcctgcgcc agaaatgagc cgcaaacatc tctcagatgc cggtcggctt
     7561 ccaggttttg ttgcctccgg ccgttatcca cggttggcga aggctgggaa tctcaccagg
     7621 gcgtcatcga agaacgccgg gattagcagg gagggtgggc actgttcgcg atcgcgacgt
     7681 cgtcagccag ggaagcctgg acgcaatctg ggcgtggctg aaagaccagc acatcgtcat
     7741 tcaagcgctg gtgtggctct tcttcctgcc cgtgacggca ggcctgtgga aatgggagac
     7801 aggctggccg ctggcggggc gactggtggc catcggtggt ctcgcgctcg cgacgctgtt
     7861 tgtgttcttc ccgcgcttcc ttcttggacc gcgaccctag gccgcgcgtt tgcgcaggag
     7921 ttcgagcagc ttcgcggtcg tgttccagtt gcggatggtc atcgagcggt attcgggcgt
     7981 gcccacgatg cggttgaggc gactcttcgt tcgctgagcg ctgaggcgct gcgagtagac
     8041 gacgcctggc ccgggactaa cgctgtcgac gccatcgcgc gggct
//
LOCUS       DATBRX010000073         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1921238, whole genome shotgun sequence.
ACCESSION   DATBRX010000073 DATBRX010000000
VERSION     DATBRX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921238"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1347
                     /locus_tag="VIF08_02565"
     CDS             157..1347
                     /locus_tag="VIF08_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEY8134896.1"
                     /translation="MIGSPFHDRTGPLNQKQQWREWSGYFAASVYDTSHIHEYFAIRN
                     QAALIDVSPLYKYVVRGPDAGRLVDRVLSRDATRIKPGQVVYTAWCDEHGKVIDDGTV
                     ARLDDDSFRWTAADPNLRWFELNARGLDVAIDDVSDSTAAIALQGPQSRSVLEALTGT
                     DWTDLAYFRRRTANAGKIALDVSRTGYTGDLGYELWVRVGDAVALWDRLMEVGRPFGI
                     HAAGMLALDMTRLEAGLILLEVDYTSSRSALVPDQSYSPFEIGLGRMVQLDKKADFVG
                     KRALLAEQAAGGPARQLVGLAINWESIDKLHREQNLPISVSYSTWREQRPVYAGRAQV
                     GRATSGTWSPILKEHIALASVDRTHAAPGTQLQLEWTVEGRRGRAAANVVPLPFFDPP
                     RKRA"
     gene            complement(1728..>2135)
                     /locus_tag="VIF08_02570"
     CDS             complement(1728..>2135)
                     /locus_tag="VIF08_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8134897.1"
                     /translation="ADRAAIDAAIERVGITLLRGRDVRELSLGERQLAVLAMAVAQAP
                     RLLLLDEPTVHLDLRHQVTVMELLRDLATRDNVTILAVMHDINLAAHFFGRLMLLDQG
                     RLVADGRPSGVLTPERIRVVYGVDPALVAIARG"
BASE COUNT          367 a          712 c          724 g          332 t
ORIGIN      
        1 aggacgtggc gtcgctcgag gacgagcgtc ttcaatccag cgcgggcgag atacgcggcg
       61 gacgtcaggc cgttgtgccc gccaccaatg acgatcgcgt cgtaccgacg ctcccctcgc
      121 cctcctcctg ccacacgcta actgtatctt tggcgcgtga tcggctcacc tttccacgac
      181 cggaccggcc cgctcaatca gaagcaacag tggcgtgagt ggtcgggcta cttcgcggcg
      241 agcgtctacg acacatcgca catccacgag tacttcgcga tccgcaacca ggcggcgctg
      301 atcgacgtgt cgccgcttta caagtacgtg gtgcgcggtc cagacgcggg gcgcctggtt
      361 gaccgcgtcc tgtcgcgtga cgccacgagg atcaagcccg gccaggtggt ctatacagcg
      421 tggtgcgacg agcacggcaa ggtcatcgac gacggcaccg tcgcgcggct cgacgacgat
      481 tcgttccgct ggaccgcggc ggatcccaac ctgcgctggt tcgagctcaa cgcgcgtggt
      541 ctcgacgtgg ccattgatga cgtctccgat tcgaccgctg ccatcgcgct ccaggggccg
      601 cagtcgcgct cggtcctgga ggcgttgacc ggcaccgatt ggaccgacct ggcctacttc
      661 cgccgacgga cggccaacgc gggcaagatc gcgctcgatg tcagccgcac gggctacacc
      721 ggcgacctgg ggtacgagct gtgggtgagg gtgggcgatg ccgtggcgtt gtgggatcgg
      781 ctcatggaag tcgggcgccc cttcggcatc cacgccgcgg gaatgctcgc gttggacatg
      841 acccggctcg aggcgggact gattctgctg gaggtcgact acacctcgtc acgcagcgcg
      901 ctcgtgcccg accagagcta ctcgccgttc gagatcggcc tggggcgcat ggtccagctc
      961 gacaagaaag cggacttcgt cggcaagcgc gcgcttctcg ccgagcaggc ggcgggcggg
     1021 cccgcgcggc agctcgtggg gctagccatc aattgggagt cgatcgacaa gctgcatcgc
     1081 gagcagaacc tgccgatcag cgtgtcctat tcgacatggc gcgagcagcg gcccgtctac
     1141 gcggggcggg cccaggtcgg ccgcgccact agcgggacat ggagccccat cctcaaggaa
     1201 cacatcgcgc tcgcgtcagt ggacaggacc cacgccgcgc cgggcacgca gctgcagctg
     1261 gagtggacgg tcgagggcag gcgcggccgc gcggccgcca atgtcgtgcc gctgcccttc
     1321 ttcgacccgc cgcgcaagcg cgcctagcgg ctggcgagtc tcaggcagtc cagatgggca
     1381 agccctgctt ggcgagggcg agcagagcca gatagatggg caaagcgccg gcgatgcggt
     1441 tgagagccac attcacgaaa tcggacctga gtgggtcgag agggccgaat gccgcccgta
     1501 ttcggctgga gtcgccccta gttcgccttg ccagcggcca taggaaccgt catgtcactc
     1561 gaatctgagt tgagatccaa ccgcgaggcg gcaatcgcgc ccaatcgcgg ctagctgact
     1621 ccaaatcggt gaacgtggct cacatcagcg ccgagccggc gaattctggg ttcgcgctgc
     1681 cgcccgggcg ctgctgcgtg gtccccactg cgaattggcg cgccgcgtca gccgcgggcg
     1741 atcgccacca gcgcgggatc gacgccgtag acgactcgaa ttcgctccgg cgtgagaact
     1801 ccagaaggcc gaccgtcggc taccaggcgg ccctggtcga gcagcatcaa acgaccgaag
     1861 aagtgagcgg ccaggttgat gtcgtgcatc accgcaagga tcgtcacgtt gtcgcgcgtg
     1921 gccagatcgc gcaggagctc catgacagtc acctggtggc gcaggtcgag atggaccgtc
     1981 ggctcatcga gcaacaagag gcgcggcgcc tgtgcgacgg ccatcgccag caccgccagc
     2041 tggcgctccc caagggacag ctcgcgcacg tcgcgaccgc gcaataaggt gatgcccacc
     2101 cgctcgattg ccgcgtcgat cgccgcgcgg tcggc
//
LOCUS       DATBRX010000074         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_22194, whole genome shotgun sequence.
ACCESSION   DATBRX010000074 DATBRX010000000
VERSION     DATBRX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22194"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..639
                     /locus_tag="VIF08_02575"
     CDS             58..639
                     /locus_tag="VIF08_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134898.1"
                     /translation="MEMLARWARPMAIASALLLIIGVVLTGLNLLLGGWTVYAAGQVL
                     AIAALAAFWAANRGRLDGWATLGLLVLLIGLTLALPQIATIWQTYAPGAFHEVGAQHM
                     ELPVWTAPFGLTAELVTWVGVAFFGLAARGAHALPRGIGWVMAGAAAIGIIAALYIVS
                     PYAWVAAMLLFAVALLSVGVSLVQPSSRMANGG"
     gene            673..>4110
                     /locus_tag="VIF08_02580"
     CDS             673..>4110
                     /locus_tag="VIF08_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8134899.1"
                     /translation="MTLICTNCGTQNLAGAKFCMECAIPLASGCPNCGFVNLPNAKFC
                     SECATPLIAGSTPAPRAVAAASTAERRLVSVLFADLVGFTPFAEERDAEDVRDTLSRY
                     FDLSRELIERYGGTVEKFIGDAVMAVWGAPTAREDDAERAVRAGLDLVGAVRSLGPTI
                     QARVGILTGEAAVTLGATNQGMVAGDMVNTAARLQGVAEPGSVLVGESTQRAAGESIV
                     FEKVADQQLKGKQAPVAAWRAMRVVAQRGGRGRSETLEAPFVGRDDEMRLLKELFHAT
                     NRERKPRLVSVIGPGGIGKSRLAWELLKYIDGLTETVWWHDGRSPAYGDGITFWALGE
                     MVRGRAGLLETDDEQTTREKVGATVRDNVPDESERPNIERALLALLGFESGFDASHLF
                     GAWRMFFERLAEVNPVVMVFEDLHFADPGLLDFIDHVQEWSRHSPITIVTLARPELLE
                     KRPTWGAGKRSFSSIYLEPLTPEQMRELLAGLVPGLPEKAAAAIVARADGIPLYAVET
                     VRMLLADARLTLSDGAYRPTGELDDIAVPETLTALIAARLDGLEAADRSLIADAAVLG
                     QSFSPAALAALSGVAAGELETRLAALVRRELLQQEANPRSPELGQYVFVQALIREVAY
                     NTLSKKDRKVRHIAAARHLESLATDEAAGALASHYVAAHGLAGEGPEAEALAAQARLA
                     LRGAAERAAALGSHEQAVTLFEQAVEMTSDTAERAELLERAYASSDQGIDVQRNMRLA
                     EAALAARREQGDRAAIARAAARVSHVVRGNLGDPERAHELAMHAWEEFSDLAETPQGV
                     KVIEALAKSLSTMGRRAEALPLADRMIAIADRLDLLEDLAGAIVSRGQLLLNMDRPSE
                     GMVLMRGGHAMALAGGFRAPELSARTLLTFREQWGEPVEGVRLAREGFDIARQIGSTL
                     YGWSMVGNGVVCALRVGEWDWAAAVIEEWIPAAMSGSDTPAQISEFYVDRAILTSLRG
                     GDPTSDLELATTARGPMTDTQYESYDSWARAWAAFCAGRFEEARSEGLRAAERTVYFY
                     PLAVPLAARAALWAGDPLSARDAITRGHTSHRGAALSLDKRTIAAGVAALEGHMAEAL
                     AMYREVLRGWREIGAAFDEALTVVDMVTFLGADEADMRSFAQWARTTLTRLGAQPFLD
                     "
BASE COUNT          606 a         1380 c         1456 g          668 t
ORIGIN      
        1 ctatcgtagc cgccgatcag ccgtccacct acccccatat ccgctacgct cggcttgatg
       61 gaaatgctgg cccgctgggc acggccgatg gcgatcgcgt cggcactgtt gctgatcatc
      121 ggcgtcgtgc tgacgggcct caatctgctt ctgggcggct ggactgttta cgcggcgggg
      181 caggtcctgg ccatcgcggc ccttgccgcg ttttgggccg ccaatcgggg ccgattggac
      241 ggctgggcga cgctcggcct gctggtgctg ctcatcggcc tgacgctggc gttgccgcag
      301 atcgccacga tctggcagac ctacgcgccg ggcgccttcc acgaggttgg tgcgcagcac
      361 atggagctgc ccgtctggac ggcgcctttt gggctcacgg ccgagctcgt gacatgggtc
      421 ggcgtcgctt tctttggcct ggcggcgcgc ggcgcccacg cgctgccgcg gggcatcggc
      481 tgggtcatgg cgggcgccgc ggcgatcggc atcatcgccg cgctgtacat cgtctcgccc
      541 tacgcctggg ttgcggcgat gctcctgttt gccgtcgccc tgctgagcgt tggcgtgagc
      601 ctggtacagc cgtcatcgag gatggcaaac ggcggttagt tatcctctgc cgcggggggc
      661 gcttcgaggg gcatgacttt gatctgcacc aattgcggga cgcaaaacct ggccggagcc
      721 aagttctgca tggagtgcgc catcccactt gccagcggct gccccaactg cggcttcgtc
      781 aacctgccca acgccaagtt ctgttctgag tgcgccacgc cgctcatcgc cggttccacg
      841 cccgcgccgc gtgccgtggc cgccgcatct actgccgagc gaagactggt cagcgtcctc
      901 ttcgccgacc tggtcggctt cacgccgttc gccgaggagc gcgacgcgga ggacgtgcgc
      961 gacacgctgt cgcgctactt cgacctgtcg cgcgagctga tcgagcgcta cggcggcact
     1021 gtcgagaagt tcatcggcga cgcggtcatg gccgtctggg gagcgcccac cgcgcgcgag
     1081 gacgacgccg aacgcgcggt tcgcgccggg ctcgacctcg ttggcgccgt taggtcgctt
     1141 ggccccacca tccaggcgcg cgtcgggatc ctgaccggcg aagcggcggt gactctgggc
     1201 gcgaccaacc agggcatggt cgcgggcgac atggtcaata ccgcggcgcg actgcagggc
     1261 gtggccgagc cggggagcgt gctggtcggt gagtcgaccc agcgtgcggc tggcgagtcg
     1321 atcgttttcg agaaggttgc cgaccagcag ctcaagggca agcaggcgcc ggtggccgcg
     1381 tggcgggcga tgcgcgtcgt cgcccaacgt ggcgggcgcg gccggagcga aacgctggag
     1441 gcgccgttcg tcggccgcga tgacgagatg cggctcttga aggagctatt ccacgccacc
     1501 aaccgagagc gcaagccgcg gctcgtcagc gtcatcggtc caggcggcat cggtaagagc
     1561 cgcctggcgt gggagttgct gaagtacatc gacggcctca cggagaccgt ctggtggcat
     1621 gacggccgct cgcctgcata cggcgacggg atcaccttct gggcgctggg cgagatggtc
     1681 cgcggccgtg ccggcctgct cgagaccgac gatgagcaga cgacacgcga gaaggtcggc
     1741 gcgaccgtcc gtgacaacgt ccccgacgaa tctgaacgcc ccaacatcga gcgcgcactg
     1801 ctggccctgc tgggcttcga atccggcttt gacgcgagcc atctattcgg cgcctggcgc
     1861 atgtttttcg aacggctagc tgaggtcaac ccggtggtaa tggtcttcga ggacctccac
     1921 ttcgccgacc ccggcctgct cgacttcatc gaccatgtcc aggagtggag ccggcactcg
     1981 ccgatcacca tcgtcaccct ggcgcggccg gagttgctcg agaagcggcc cacctgggga
     2041 gccggcaagc gcagcttcag ctcgatctac ctggagccgc tgacgcccga gcagatgcgc
     2101 gagctgctcg ccggcctggt gccggggctg ccggagaaag ccgcagcagc gatcgtcgcc
     2161 cgtgccgatg gaattccgct gtacgcggtc gaaaccgtgc gcatgctgct cgcggatgcg
     2221 cggctgacgc tcagcgatgg cgcttaccgg ccgaccggcg agctcgacga catcgccgtg
     2281 cccgagacgc tcaccgcgct cattgccgcg cggctcgacg gcctggaagc ggccgaccgc
     2341 agcctgatcg ctgatgcggc ggtgctcggc cagagcttct cgcccgcggc gctggcggca
     2401 ctgtcgggtg tggcggcggg agagttggaa acgcgtctgg ccgcgctggt ccgacgcgag
     2461 ctgctccaac aggaggccaa tccgcgctcg ccggagctcg gccagtacgt cttcgtccag
     2521 gcgcttatcc gggaagtggc atacaacacg ctctccaaga aggaccgcaa ggtgcgccac
     2581 atcgccgccg cgcgtcacct ggagagcctc gccaccgacg aggcagccgg tgccctggcg
     2641 agccactatg tggctgccca cgggctggcc ggggagggcc cggaagccga agcgctcgct
     2701 gcccaggccc ggctagccct gcggggagcg gccgaacgcg cggctgccct gggctcgcac
     2761 gagcaagcgg taacgctgtt cgagcaggcg gtcgagatga cctccgacac ggccgagcgc
     2821 gccgaactgc tcgagcgcgc ctatgcctcg agtgaccagg gaatcgacgt ccagcgcaac
     2881 atgcgactgg ccgaggccgc ccttgccgcg cgccgggagc agggcgaccg cgcggcgatc
     2941 gcccgtgcgg cagctcgcgt ttcgcacgtc gttcggggga acctgggcga cccggagcga
     3001 gcccacgaac tggcaatgca cgcatgggag gagttcagcg acctcgccga gaccccgcaa
     3061 ggcgtcaagg tgatagaggc gctggccaag agcctgtcga cgatgggccg tcgcgccgaa
     3121 gcgctgccgc tggccgatcg catgatcgcc attgccgacc ggttggacct gttggaggac
     3181 ctggcaggtg cgatcgtgtc gcgcggccag ctactcctga acatggaccg gccatcggaa
     3241 ggcatggtac tgatgcgcgg cggccacgcg atggcgctgg cgggtggttt ccgcgccccg
     3301 gagctgtccg cccggacgct tctgacattc cgcgagcagt ggggcgagcc ggtcgagggc
     3361 gtccgtcttg cgcgcgaggg cttcgacata gcccgtcaga tcggctcgac actttatggc
     3421 tggtccatgg tcggcaacgg cgtcgtctgc gccttgcgcg tcggcgagtg ggactgggcg
     3481 gcggcggtca ttgaagaatg gatccctgcc gcgatgtcag gcagcgacac gccggcccag
     3541 atctcggagt tctacgtcga ccgcgcaatc cttacgagtc tgcgcggcgg cgatccgaca
     3601 tcggacctcg agttggcgac gaccgcgcgc gggccgatga ccgacacgca gtacgagtct
     3661 tatgactctt gggcccgggc ttgggcggca ttctgcgccg gccgcttcga ggaagcccgc
     3721 agcgagggcc tacgggcggc cgagcggaca gtctatttct acccgctagc ggtgccgctc
     3781 gccgcgcgcg cggcgctctg ggccggcgac cccctttcgg cacgcgacgc cattacccgt
     3841 ggccacacga gccatcgcgg cgccgctctg tcgctcgata agcgcacgat tgcggctggc
     3901 gttgcagccc tcgaagggca catggcggag gcgctggcga tgtaccgcga ggtgctgcgc
     3961 ggctggcgcg agattggcgc ggcgttcgac gaggcgctga ccgtggttga catggtcacc
     4021 ttcctgggtg ccgatgaggc cgacatgaga tcctttgccc agtgggcgcg taccacgctg
     4081 accaggctcg gcgctcagcc gttcctcgac
//
LOCUS       DATBRX010000075         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1809965, whole genome shotgun sequence.
ACCESSION   DATBRX010000075 DATBRX010000000
VERSION     DATBRX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809965"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VIF08_02585"
     CDS             <1..600
                     /locus_tag="VIF08_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8134900.1"
                     /translation="LGIERFSIVAWSGGGPYGLACGFALPDRVDAVAAVSPAAPLAKP
                     TDPGYLRWRDRHAVRFAKHSPWVIRLAMWHWGRGQRGDAEKFFERSVADMCAADQAVL
                     AEPELRSRMIDNSAELYRQGGRGMYDEALVLTKPWGFEPAELTVPVHIWQGGRDDTVP
                     EAMATHLAEVIPGASLRTYPDEGHHLLYRYWPEILADLA"
     gene            complement(694..1851)
                     /locus_tag="VIF08_02590"
     CDS             complement(694..1851)
                     /locus_tag="VIF08_02590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEY8134901.1"
                     /translation="MERVLILGGGVGGTIVANLLSRKLRRQIEDEDVAVTVVDQLGNH
                     LYQPGFMYIAFGNEQERNLSRPERSLLDSRIKLIVGEATGVNADARTVTLADGQVLVY
                     DQLVLATGSRIAPEEIEHFDTDAHHFYTADAARRLRTALDAFTGGSIVVGIAGMPYKC
                     PPAPLEVSFLIEAELRARGLRDRSEVHFCSPIGRAFTIETVSEMATPVLEEKGIELHT
                     FFNVEAIDPARKVVQSLEGEELHYDLLILVPPHHGAQFLIDSGLAPAPGGWLPTDHHT
                     LLVGGRSNVYALGDATDLPLSKAGSTAHFEAPVVAERIAAKLTDRPLDEKHGNYTGRV
                     MCFFEIGDGKGTLLRFDYDHPPQPPKPNRVWHLGKMLFNKTYWHTVPRGRV"
     gene            complement(1851..2108)
                     /locus_tag="VIF08_02595"
     CDS             complement(1851..2108)
                     /locus_tag="VIF08_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY8134902.1"
                     /translation="MTTPTQQLIKPVDRVIDARGSFCPGPLMELIRAIRESEVGTVIA
                     VRSSDRGSRIDIPKWVEKAGHRLLGVSPAEGFDEIVIEKLH"
     gene            complement(2126..2728)
                     /locus_tag="VIF08_02600"
     CDS             complement(2126..2728)
                     /locus_tag="VIF08_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY8134903.1"
                     /translation="MTAPVAEPETIISPETELERDLLRGLLEEAPDDEPQTEAETPVR
                     QLVIINWSGEFDRVMPTLIMANAAAAMGYQTKVFTTFWGLLPFVKDQKRIVGENWMQK
                     MLAFMQRPGISHLKTSKMNFFGMGPWMMGVLSRKYNVASPKELLEAAEALGVEFMPCQ
                     MTMDMFGLKREDMIDGLGAPVGAATVLELMTQPNTASLFI"
     gene            complement(2739..2996)
                     /locus_tag="VIF08_02605"
     CDS             complement(2739..2996)
                     /locus_tag="VIF08_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEY8134904.1"
                     /translation="MTLTENNPAGTVKVTTLDAKGLKCPMPIVKTAQAIKTVGSGDLL
                     EVLTTDPGAVADFAAWSRATGNTLVEQTLVEGVYRFVLRRK"
     gene            complement(3014..3439)
                     /locus_tag="VIF08_02610"
     CDS             complement(3014..3439)
                     /locus_tag="VIF08_02610"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEY8134905.1"
                     /translation="MNDQAEEPINLVLFSGTDDRLTAAAVLAAGGAALGRPVHIFLQY
                     WALDAFRADKMGARLPLAPEAGVAGAAAVEHMRAAGQQPWVETLRQAKDIGEVDIQAC
                     SLSMDLLSLSADQLDPIVDGVEGVAAFYVNAGAGQIVFI"
BASE COUNT          570 a         1200 c         1272 g          647 t
ORIGIN      
        1 ctgggcattg agcggttctc gatcgtcgcg tggtcgggcg gcggccccta tgggctggcg
       61 tgtggtttcg ctctccctga ccgcgtcgat gcggtcgcgg ccgtttcgcc ggccgcgccg
      121 ctggcaaagc cgactgatcc gggctatctg cgctggcgcg accgccacgc ggtccgcttc
      181 gccaagcact cgccgtgggt catccgtctc gccatgtggc actggggccg cggtcagcgg
      241 ggcgacgccg agaagttctt cgagaggtcc gtcgcggaca tgtgcgccgc cgaccaggcg
      301 gtccttgccg agccggagct gcgcagccgc atgatcgaca actcggccga gctgtaccgc
      361 cagggcggcc gcggcatgta cgacgaggcg ctcgtcctga ccaagccgtg gggcttcgag
      421 ccggccgagc taaccgtgcc ggtgcacatc tggcagggcg gccgcgacga cacggtccct
      481 gaggcgatgg ccactcacct cgccgaggtc atccctgggg cgagcctcag gacgtacccc
      541 gacgaagggc accacctgct gtatcgctat tggccggaga tcctcgccga cctggcctag
      601 acaaaggccg acggcggtcg tgctgcggga cgaccgccgt cgggagggac atgcgtattc
      661 ggttggtgcc gctcaaagcg gcactgtttc gggtcagacc cggccgcgcg ggaccgtgtg
      721 ccagtaggtc ttgttgaaga gcatcttgcc caggtgccac acccggttgg gcttgggcgg
      781 ctgtggcggg tggtcgtagt cgaagcgcag cagcgtgccc ttgccatcgc cgatctcgaa
      841 gaagcacatg acgcggccgg tgtagttgcc gtgcttctcg tcgagcggcc tgtcggtgag
      901 cttcgcggcg atgcgctccg ccactacggg cgcctcgaag tgggcggtgg agccggcctt
      961 tgacagcggc agatccgtgg cgtcacccag ggcatagacg ttcgaccgtc cgccgacgag
     1021 cagggtgtgg tgatcagttg gcagccagcc gcccggcgcg ggtgcgaggc cggagtcgat
     1081 caggaactgg gcgccatggt gcggcggcac gaggatcagc aggtcatagt gcagctcctc
     1141 gccctccagg ctctgaacga ccttacgcgc tggatcgatc gcctccacgt tgaagaaggt
     1201 gtggagctcg atgcccttct cctcgagcac cggtgtcgcc atctcactga ccgtctcgat
     1261 cgtgaacgcg cggccgatgg gcgagcagaa gtgaacctcg ctcctgtcgc gcaggccgcg
     1321 cgcacgcagc tccgcctcga tcaggaacga gacctcgagc ggcgccggtg ggcacttgta
     1381 cggcatgccc gcgatcccga cgacgatgga gccgccggtg aaggcatcaa gcgcggtgcg
     1441 caggcggcgc gcggcgtccg cggtatagaa gtgatgcgcg tcggtgtcga agtgctcgat
     1501 ctcctcgggt gcgatccgtg agccggtggc caggacgagc tggtcgtaca cgaggacctg
     1561 gccgtcggcc agcgttacgg tgcgcgcgtc cgcgttgacg ccggtggcct cgcccacgat
     1621 cagcttgata cgactgtcaa gcagcgatcg ttccggccgc gagaggttgc gctcctgctc
     1681 gttgccaaag gcgatgtaca tgaagcccgg ctggtacagg tggttgccca gctgatccac
     1741 gacggttact gcgacgtctt cgtcctcgat ctggcggcgc agcttgcgcg acagcagatt
     1801 ggcaacgatc gtgccgccaa ccccgccgcc caagatcagg acgcgttcca tcagtggagc
     1861 ttctcgatga cgatctcgtc gaagccctcg gccgggctca cgcccaggag gcgatggccg
     1921 gccttctcga cccacttggg gatgtcgatc cgcgagccgc gatcggacga gcgaacggcg
     1981 atgaccgtcc cgacctcgga ttcgcggatt gctcgaatga gctccatcag gggccccggg
     2041 cagaagctgc cgcgggcgtc gatgacccgg tccacgggct tgatgagctg ttgagttggc
     2101 gtggtcatgt gcgccacttc cttccctaga tgaagaggct ggcggtgttg ggctgggtca
     2161 tcagctcgag gaccgtcgcc gcgccgacgg gcgcgcccag gccgtcgatc atgtcttcgc
     2221 gtttcaggcc gaacatgtcc atagtcatct ggcagggcat gaactccacg cccagcgcct
     2281 cagccgcttc gagcagctcc ttgggcgagg cgacgttgta cttgcgtgac agcacgccca
     2341 tcatccacgg gcccatgccg aagaagttca tcttcgaggt cttgaggtgg ctgattccgg
     2401 gccgctgcat gaaggcgagc atcttctgca tccagttctc gccgacgatg cgcttctggt
     2461 ccttgacgaa gggcagcagc ccccagaacg tggtgaagac cttggtctgg tagcccatcg
     2521 ccgcggccgc gttggccatg atcagggtgg gcatgacccg gtcgaactcg ccgctccagt
     2581 tgatgatcac gagctgcctg accggcgtct ccgcttcggt ctgcggctca tcgtcaggag
     2641 cttcctcgag gaggccgcgc agcaggtcgc gctcgagctc ggtctcgggg ctgatgattg
     2701 tttcgggttc ggctacaggc gcagtcatat cggtaacctc acttcctgcg caggacgaag
     2761 cgatacacgc cctcgacgag ggtctgctcg acaagggtgt tgccggtcgc gcggctccac
     2821 gccgcgaaat cggccacggc gcccggatcg gtggtcagca cctcgagcag atcgcccgac
     2881 ccgacagtct tgatcgcctg ggccgtcttg acgatgggca tggggcactt gagacccttg
     2941 gcgtcgagcg tggtcacctt gacggtgcca gcgggattgt tttcggtgag cgtcattgcc
     3001 ctcactcctt gcgttagatg aagacgatct ggccggcgcc ggcgttgacg tagaacgcgg
     3061 ccacgccctc caccccgtcg acaatggggt cgagctggtc ggcagagagg ctcagcaggt
     3121 ccattgacag cgagcacgcc tggatgtcga cctcgccgat gtccttggcc tgacgcaggg
     3181 tctcgaccca gggctgctgt ccggcggcac gcatatgctc gacggcggct gctccggcaa
     3241 cgcccgcttc gggcgcgagc gggaggcgcg cgcccatctt gtcagcgcgg aacgcgtcga
     3301 gcgcccagta ctggaggaag atgtgcaccg ggcggcccag ggcggcgccg ccagcggcaa
     3361 gcacggcagc cgcagtcagg cggtcatctg tgcctgagaa cagcacaagg ttgatgggct
     3421 cttcagcctg atcgttcacg atcggctccg cagcggtttc ctacggccta acgatggccc
     3481 cgtgcacgtt ggcgttcgag tgcccgggcg ccccttggaa cgggccatgt gacccgcccg
     3541 caagggccat tcgggtccgc gctgagcggg gagcgcctca gtgggcgacg gtcgcggcag
     3601 cctcggctat gacgatgtcc tcgcgcgccg cgacgcgcag cacggcaata ccagcctcgg
     3661 gcgctgaaag caggccgtcg ccgctcccc
//
LOCUS       DATBRX010000076        25794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3487188, whole genome shotgun sequence.
ACCESSION   DATBRX010000076 DATBRX010000000
VERSION     DATBRX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 25794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487188"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VIF08_02615"
     CDS             <1..522
                     /locus_tag="VIF08_02615"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEY8134906.1"
                     /translation="EWGVESTGQSEVVLTRSAGGLSVRKSIAVFHGRMNGSLDLDIVV
                     TADSAFRGPLELEWNLNLLGGGANPEAHYHWADQEIRHDAPGSVAAGVQLSFGNRYEG
                     VAISVQLEPAAPQQWFPVETVSNSEAGFERVYQGSCLTQRWPLELAAGESKRFTVTMA
                     FKQTRDRAGEEAG"
     gene            complement(723..1499)
                     /locus_tag="VIF08_02620"
     CDS             complement(723..1499)
                     /locus_tag="VIF08_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8134907.1"
                     /translation="MRAIELERTRWVTLLGARVERDDALGVAWAAHPARGAGLNFASC
                     IRWPAADVNARLAAVDARMRASNTWPQVVVAEGLTEPHDLESRLRAAGWVPLGSERLM
                     FTRHASVVPHLDPGLRIEAVTPATALECATLESAAFGLDPDGIDVSAGLLALVVESGD
                     TRAFIVRLAGEPIACARLVPGEPGSGVASLHAVGVAERHRRRGYGRLITAIATRAGLA
                     TGHKLVWLSVDADNAGAITMYRGLAFDFTFSWTRWAAPVR"
     gene            complement(1601..2482)
                     /locus_tag="VIF08_02625"
     CDS             complement(1601..2482)
                     /locus_tag="VIF08_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8134908.1"
                     /translation="MDFGLILPSYRPGASVEGIEAASETSGRLGWHSAFSTDHFLVGT
                     SAREEDYFHIFDALATVVHVAARQPTLRVGVSVLVVPMRNAVELAKELATIDVLSGGR
                     LIVGIGGGWDEKEFAHLGLADRFGQRGAYLNEAIAIWRHLWGGGQGPFHGRFHSFEEV
                     RFGPLPVQGAELEIWVGGRDEAALRRAGQLANGYHSSATGPDHYAPRVPVVRAAAAEA
                     GRPEPRFSARFRVAFGEHSVPYFMLSGTPDQMIATLREFAALGVTHVAVDFAETDPDK
                     VARLIERFDSEVRAAFL"
     gene            complement(2495..4276)
                     /locus_tag="VIF08_02630"
     CDS             complement(2495..4276)
                     /locus_tag="VIF08_02630"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8134909.1"
                     /translation="MSLPPDNRPTPVTAFDHALKAFLDDFFAAQPVWATQIGFHAHDD
                     RWPNMTEAGRSARLAMLRDHAGRFEALAASGLSADEQIDRGIVAEAIEGMIFEDEQLR
                     EHAWDALSYVWQAGSGLFGLLAREYAPWAHRGTAFAGRLRNLPAFLEGAAASLTGLPG
                     RPVSRLHAETALLQMDGITELIDDGIAEAEKRAGDDREVAAAIHAAAEPARAAVARYA
                     EALRDEIMPRAEGEGRLGADLFAAKLRHTTSSDLPPGELGARARRDYDLVRAEMLRLA
                     RLVWPEWLPGEEMPADTDELIRRALFAVAQEHRQPAELLDFSRAEVRRIEDFCRANDV
                     IGLPDEPMMITWTPVFMRAFGRAFLNAPGVLDKGQSSYFWITPPDDSLGAEATESYLR
                     EDNDRMLRLLAIHEGVPGHYLQGAYANRSPSLARAVFGSGMFAEGWAVYVTQVLMDMG
                     YGEHEPALLLNHWKFYLRAITNALMDVAIHGEGMTEEQAMELMVGGGFQERDEATAKW
                     LRARLTSTQLTTYYLGSLEMWALEEGARRRAAVAAGAGADVVPPQRIAGGMGETPGFD
                     YRAHLESVISHGSPPIKWLKRALFGSD"
     gene            4319..4726
                     /locus_tag="VIF08_02635"
     CDS             4319..4726
                     /locus_tag="VIF08_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8134910.1"
                     /translation="MLLDLLLRVAINAIALILAVQFVPNVRAPSDILKLVLLAVVFGL
                     INAYLRPIVKALSLPLTIVTFGLVSLVINVAMVLVAAAISDNLNLGFALGGWPLNDPS
                     IGIDTLIAAFLVSLLISIVAAVVALVRRLTPGI"
     gene            4782..6731
                     /locus_tag="VIF08_02640"
     CDS             4782..6731
                     /locus_tag="VIF08_02640"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEY8134911.1"
                     /translation="MSGTSSSQRDAFAARLAKAPLLLDGAMGTLLYSRGVPQRASLDQ
                     LVEVRPDLIGAIHREYIEAGADAIETHSFGANRLRLAPFGLADKAALFNRRAAQIARE
                     ARDVAGKRDVLVAGSIGPLEAPLHGPDAAKEEAIRAAFGEQIEGLLEGGVDFLLFETF
                     SDLRELLIGISAAKALSDLPIVAQMTFGEELLAVDGTSPQTAATALAAAGVDAVGVNC
                     GVGPQICLDALTQMGRPAAGLARSILPNAGLPQRVEGQFVYAAEPAYFAAEVPRFLDA
                     GARIVGGCCGTTPEHIRHMRQALDAYNAHKQSATASVEAPEIGGTPRTPVAERPSGSI
                     AEPPPPTRLAQLLTQDTFILSVEIDPPRSVRIERTIEAARLLQDAGVDVVNISDSAMA
                     RVRMGALAVAFGVQHDLDLECLVHFTTRDRNLMALESELLGAHALGVRNILALTGDPP
                     RVGDYPTGTGIWDVDSVGLIGILARLNRGEDQAGKPIGSPAGFTVACALDPTADNLGR
                     EIERLAAKMDAGANVVMTQPIYSRDQWDRFMEFAAARWPSGLPVPVLLGVLPLHTARH
                     ADFLHNEVPGITIPKEVRAAMHAAGERGAEVGLEMAHRLLDSLAPLVHGTYIMPSFGR
                     YELAAELVRRIRSDTRIVTSARSPA"
     gene            6728..8479
                     /locus_tag="VIF08_02645"
     CDS             6728..8479
                     /locus_tag="VIF08_02645"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY8134912.1"
                     /translation="MTRTRFARLVRSALAAGWLLLIGGSAVLAADPPLAGPPFPAPQA
                     DVVVYDYADILSPATEARATQSITDIEHRTGAEIVVYTQYKPGSDEDSTEQDAIDLID
                     QWGVGRAGFDDGLAIMWNTGRLECMPGVSGNGQVQLYAAPGYRAAYLSNGERQSIFDN
                     DMLPYLRQCDEDSALLAALDKIDAAATPEHAQMLDRARIIDAAIGLILGPGIFILLVG
                     WVSISWLRYGKDPVYLDDPSVLMPAPPPDLTAASGAVIWDGRSSRRALTTAMLDLASR
                     GEIGFKPESELLGLRTKAGIQINETYDADDPLIDRNRRKPLSKAEQYALERLRGIASP
                     LTGNYIGASDLLKFGKYVSGFDKQIEAHVVSKGWFHEAPAKATERWASRAILILIAGF
                     VGLIIGVNIPSGGVVVFTVALMAAAFVMLFIARSMPARTMAGAMIYAMLAAYRRTMEK
                     TMAQARSMNTVVEEPALAWMETPDQAVAWGVALGLQRDVEEVLARSAEDAQAGVTTYH
                     PYLPAWYGGGLTSGQGGQGGFAPGLMSSSAIPNFGGMMAALSSIGNSPSSSGSGGSGG
                     FSGGGSGGGGGGAGGGY"
     gene            8515..8844
                     /locus_tag="VIF08_02650"
     CDS             8515..8844
                     /locus_tag="VIF08_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134913.1"
                     /translation="MGATETMEQFFELLNNGQRDDALKLMDEKIEMRVHVGDNARTLR
                     GVEQVGGWFLRADKGMRMIPANERDMGVNLEIDLMVMRPGVQSQHLDATFRVEAGKIT
                     AINFAPR"
     gene            complement(8877..10067)
                     /locus_tag="VIF08_02655"
     CDS             complement(8877..10067)
                     /locus_tag="VIF08_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8134914.1"
                     /translation="MSQRGDLFGEGPQACPFIALELDRDRRSDKPDYRHRCFAEPTPQ
                     PRAIAHQEAYCLSTEFAACPIFQGWAMRAAARPVPTPAGYEGRPGQSQAPVPTVAEVQ
                     AAAAQAPLPVVEPIVPAPGEAWPVEAFSPAAAVDAPAQLAVFESDAPVEPASPAEQEF
                     PSAWSTVSASPVAPAFLAGASAGPADETPDDDFDDAPVPGFLAGRTERTPGAQPRASR
                     PDVQLRETVSREDVVPSWDLTDRYGADLDEHAHRRGRDDDGGRGGDRISGLMTAIAVV
                     AILALGVAGVIFLPGMLAGSPRATATPATTLGPTFLTSPSVPAASTTPVTSIGPTVAP
                     TATAEPDATPRLYRIKNGDTLGKIAKRFHVTVADIMAANPEITDPDHIVPGQVIVIPP
                     SSGS"
     gene            complement(10103..11431)
                     /gene="cysN"
                     /locus_tag="VIF08_02660"
     CDS             complement(10103..11431)
                     /gene="cysN"
                     /locus_tag="VIF08_02660"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEY8134915.1"
                     /translation="MDLLRFSTAGSVDDGKSTLIGRLLYDSKGIFEDQLAAIEKTSRR
                     RGYGQVDLALLTDGLRAEREQGITIDVAYRYFATPRRKFIIADTPGHVQYTRNMVTGS
                     STADLAIVLIDARHGVLEQSRRHAFIATLLGIPHLVVAVNKMDLVSYSEETFNAIREE
                     FATWAQKLDVHDIQFIPISALHGDNVVTRSLSMPWYQGPSLLYHLESVYIGSDRNLID
                     ARFPVQWIIRPQTDEHHDYRGYAGQIAAGVFRKGDQVTILPSGKTSRIARIETMDGEL
                     EAAGPPMSVSMLLEDDIDISRGDMICRPHNQPTVANELDAMMCWMSESELKPGGRYLL
                     KHTTRTVKAVVDDLRYRIDVNSLHRDQAADSLGLNEIGRIHVRTSAPLLLDPYDLSRT
                     TGGFILIDEATFDTVGAGMVIHAGPEKQQMPLESALETDTDDAARVPAAA"
     gene            complement(11435..12370)
                     /gene="cysD"
                     /locus_tag="VIF08_02665"
     CDS             complement(11435..12370)
                     /gene="cysD"
                     /locus_tag="VIF08_02665"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY8134916.1"
                     /translation="MIPLEAQPDAMARANITHLDALEAESVFIMREVAAEFERPVLLF
                     SGGKDSIVMLRLAEKAFRPAKFPFPVMHIDTGHNFPEVLAYRDRTIERLGERLIVASV
                     QESIDKGRVREEPGNPSRNRLQTTTLLDAILEHQFDAVFGGARRDEEKARAKERVFSL
                     RDEFGQWEPKAQRPELWSLYNGRLNKGQHMRVFPISNWTEMDVWQYIQREEIEVPSIY
                     FSHERDVFRRDSMWLAVSDFLPPKDGEEVQRMTVRYRTVGDMTCTGAVDSRAASLEDI
                     VLEVAAARITERGATRADDAFSETSMEDRKREGYF"
     gene            12439..13368
                     /locus_tag="VIF08_02670"
     CDS             12439..13368
                     /locus_tag="VIF08_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8134917.1"
                     /translation="MSDAQAPPRSADSSRLDIWAERIGVSRRTLIAGIVLPIISLIAI
                     CVTFALAPADQRGLIFFSTLVALAAGLSSTAIGVYGGVLVPGLLLLGVDARFAAAASL
                     FLQVLVIPLAASSHYRMGNFSRNVALPLLIGGVIGAFIGPFFAAAIPKEWIVRFVAAM
                     IVFVGIIVLATLHLTGLGKVRADDDIPQAQVGGIGLLAGFSSGVSGAGWGPIGVKLLI
                     LSRIDPRQAIGSSLFARIFMAAAAVVGFFVAQTAFNNATANWWIVVPLFAGSVAPMIA
                     GAMLVSRLGRERATVAITLLSIALALPSLLWGH"
     gene            13438..13872
                     /gene="sodN"
                     /locus_tag="VIF08_02675"
     CDS             13438..13872
                     /gene="sodN"
                     /locus_tag="VIF08_02675"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEY8134918.1"
                     /translation="MFRILSALAPSATAHAHCDIPCGVYDPQQARIEAESCYRIIEKY
                     AASSDELFRARCIVVKEERAELAKHHIDVMWHDYFKPEHVEKFPTLHETCWKAAKQAS
                     LVKRSLDIAEAQKLLDLIDKIDHMWRETGGPAATRMPAAASA"
     gene            13872..14189
                     /locus_tag="VIF08_02680"
     CDS             13872..14189
                     /locus_tag="VIF08_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEY8134919.1"
                     /translation="MRRLVRSWRHRVVVDGHSMEPTFHDGDWLLVDPDARPGLGDLAV
                     ARVAERLILKRVVDIDEGGRYTLAADNPVHAGQRIGPLEPSLVIGRPWFRYGPRRRYG
                     PLD"
     gene            14256..14717
                     /locus_tag="VIF08_02685"
     CDS             14256..14717
                     /locus_tag="VIF08_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8134920.1"
                     /translation="MPVAAPEDIVEQILDQLEPLVAHQRRAVAKEGCLRAISSTQLHV
                     LFMLLSDGPTTMSRLAESLNVSLPNVTGIVDRMVEHGLVERGRADDRRVVTVLATDAG
                     RHTVEEIDLLRRRRLTALLAQLDPDQQRRALETFTELRLAAERLDRQGAPA"
     gene            14720..16399
                     /locus_tag="VIF08_02690"
     CDS             14720..16399
                     /locus_tag="VIF08_02690"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY8134921.1"
                     /translation="MEATPPTTEGASATPAVQIDHRVRTEILIGILLALFLSALDQTI
                     VGTAMPRILTDLHGIDLYTWVVTIYLLTSTITGPIYGKLSDQFGRKNLLIFGVVVFLV
                     GSALSGLSQDMIQLIVFRGIQGIGAGAIFPIALAVIGDLFTPRERGRYQGLFGAVFGI
                     SALVGPALGGFLTDSISWHWVFYVNIPIGAVALFIIWRLLPPLHTAGVTRKIDYLGVA
                     VFTAALVPILIGLTNAQQHDWTDPLVGGLILIGLALGGLFLWIETRAAEPIVPLGLFR
                     NRTYAISIVATFLASFGFFGAVLFLPLWYQVVNGSTATESGYQLLPLLGGLIISSIAS
                     GQIVSRSGRYKWLTVGALVVLSIGLLLLTNLRAETPPPMLWLWQFVTGLGVGPTFAVF
                     TIIVQNAVPWNQLGVATSNLTFFRQIGGTVGLSLAWTIFGSTLRTEAPTQVTAQLVNA
                     GVPQQQITLFTSNFTLSGQQLNDFGSVGDMGARILAQLPEQARQFVEPLIGYIVAGIH
                     QALSIAIANSMWLGVGAALIAVFVAALMPELPLRRDHNPDSAALKAAPPTP"
     gene            complement(16396..17349)
                     /locus_tag="VIF08_02695"
     CDS             complement(16396..17349)
                     /locus_tag="VIF08_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8134922.1"
                     /translation="MRFALMTEPQQGLSYTEILALARAAEAAGFEAFFRSDHYASFPG
                     GAGLPTTDAWATTAGLARETTRINLGVLVSPVTFRAPGNLAKVSATVAEMSGGRVEVG
                     LGAGWNEPEHHQQGLAFPDTRERFDMLEEQLAIVHGLWTEPDGWSYTGAHWQVSDSLF
                     RPRPPAPVGRKHPNLIVGGEAKARMAALVARYADEINITSATPGSASAGYARVDEACR
                     ATGRDPRDVTRSAMTGVLVGRDEADVERRAQALLASVNSGGLQGERWLDERRRRWIFG
                     TPEQARARVAELGAVGVERVMLQTFLPRDLEMVTLLGEVFL"
     gene            complement(17410..18342)
                     /locus_tag="VIF08_02700"
     CDS             complement(17410..18342)
                     /locus_tag="VIF08_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY8134923.1"
                     /translation="MRGRRLVLAAAAVLLVGLVAACSGAAAAPTPGGDRTGDGNGYQP
                     APTAAPGNGNNPFPNGNNFIPEQPQLIVYNGSLDLEVADVDTSVSQAEALVKGLGGHV
                     AGSRASDSGNGKSAYVTYRIPAARWSEALSGLKGLATRVANEETSSDDVTAQVVDLEA
                     RLANLRATEAALQGIMARANTITDVLKVQAELTQVQGDIESMTAQRDLLTNQAALATL
                     NVAFNPPPVAEVTQASTGWDLGREIDGALASLVRIGQGLASLLVWLVIVVLPVVGPVV
                     IGIWVVNRLWLRWRRLHPVAQPGAGPSGPWPPSM"
     gene            18465..18956
                     /locus_tag="VIF08_02705"
     CDS             18465..18956
                     /locus_tag="VIF08_02705"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8134924.1"
                     /translation="MTTDPDARVMPIPAGLVAFGAATAEFVGLAQVADLPAGTMLRVT
                     RGDHDLLLVNGQGGICAIDDRCPHMAAPLSLGSLEGCVIGCPLHKGKFDLCDGGIVQF
                     PTTGGLDPDGGYHAPWTPADAPPKPEPTDLKAMARAATRVRRLRYYPVHIVDGRIEVR
                     LPG"
     gene            19008..19613
                     /locus_tag="VIF08_02710"
     CDS             19008..19613
                     /locus_tag="VIF08_02710"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8134925.1"
                     /translation="MAGRIRIGGVLSGKVRASREVGREGSRSLRTVVTGEPCVDFRTT
                     RTIRSAIEIRAPLDVVWRVLTDFDAYPAWNPHVRRLVGKPQAGRRIGIHTRPPGGRTV
                     VMRPIILDWSPPHELRWRATFVSRRLFSGEHGFRLEEVAPDRVRFAQDETFSGLLVPL
                     YARLRLPSTRLGFAQMNEALRTRAEAEAAAAAPAASVGAGA"
     gene            19709..21487
                     /locus_tag="VIF08_02715"
     CDS             19709..21487
                     /locus_tag="VIF08_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEY8134926.1"
                     /translation="MTHPRPSAWALICVLVAGLLGSAGTGVTLAVDKPTQCTGWTDEF
                     HPPTEIRVYRNDGPNAGHVEVVPFRDYVGTVLAAEYSTAGPKGAIWFRMGAVSVKQYG
                     WYYTMHYRGGKVTNYNEDGTVASYDCWDVRDSTIDQLYKPVKLVDGQWVPRNVPSAAN
                     LSAIDETWHISLRKWMADKNKSRFFLTGYRSGKKNPCGADAKGFRIMQKSLGDCIVKG
                     LVYEETTRAYYEPNVLIVDPRERDILDDSNTYFGDVGALSLGANNTGWRVYAGLADGF
                     ADPVTGTFANITSGNFGGHGMGDVTGDGRNDLVILTLGATNKLRLAVANGIGYADPTS
                     QDLPGGAPTARMLVADFNGDLLADVGLLRSTPVQPLPDEPATMVVMRGNANGTFSAPV
                     DWWRGVLDLATQQVAAGDVNGDGKADLVMSDGVAGVTFSVAPSFASCADLTVWGPCPS
                     VAGIGLDVATQWLNKASWVAAEVKWTMTDFNRDGRTDVVAVLKDGSGIDVVGARAIKG
                     GGFADTTMMLGINSLAFGDVTPVGSEINPDGLGDLVLLKKNGANTTVQWLRASQAVGS
                     PAVTYVTTTGLNDTGLPWGPATTKAY"
     gene            complement(21481..21858)
                     /locus_tag="VIF08_02720"
     CDS             complement(21481..21858)
                     /locus_tag="VIF08_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEY8134927.1"
                     /translation="MPAQVPPVVVQVFGRRDSRDTQKALRFFRDRRVQVNFVDLGAKP
                     MAPTELRRFSARFGPANLFDTASRAYQDAGLGYLSMSADAAFEKLVRDQRLIRLPLVR
                     AGNRLSLGLAEDEWRAWLRAADQ"
     gene            complement(21861..22160)
                     /locus_tag="VIF08_02725"
     CDS             complement(21861..22160)
                     /locus_tag="VIF08_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134928.1"
                     /translation="MLDKPKAAARPRALSPKALARLKQHRQLVKAIGQIASAKDVFEI
                     RLGGDEKPLTMRQRIMRAAEEARKDIVVRKSDKGWLIGLTTPERRSKRGRRKTAK"
     gene            22265..23149
                     /locus_tag="VIF08_02730"
     CDS             22265..23149
                     /locus_tag="VIF08_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8134929.1"
                     /translation="MKIGVIVPQGWTGEYRGRIAVDAWARTRSVARQSEALGFESIWL
                     FDHFHTVPRPTLEITFEAFTSLAALAAETSRVRLGHVVLCAAYRNAALTTKMISTLDA
                     ISAGRAELGLGAGWKRDEWRAYGYGFPDAPERLAILKDTLEVARAMMTDDRATYAGKH
                     LHIAGAINRPRPVQQPRIPIMVGGNGPNVTWRLAARYADELNLDGLTPAEVKDALPTI
                     RARCEEIGRDPASLSVSVHVWWEQLVASGAARSDMIAAYRDLGISRVIALVRDSADSD
                     EALEGWAQDCRSAGAELE"
     gene            23169..24557
                     /locus_tag="VIF08_02735"
     CDS             23169..24557
                     /locus_tag="VIF08_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134930.1"
                     /translation="MHRLMSARTRLTLLGGAIGGPALLLAWPGVALAHQLNERYAAPL
                     PLLAYVGGAALAVAMSFAFIMLRNAPAPRDEVPVSNRLVPRWLRLALQGIGLIGWLWI
                     VIQTFGGGSGDGDVASLFLWVYGWVGLALISALGGPIWAWLDPFSTIHQILGAVAGRL
                     GISGGESAPYPARLGKWPAVIGLAVVVWLELVARVEGGRSLGLFLIGYTIITVAAMAY
                     FGRPAWRANGETFSVWFGVLGRLAPFALDGEPEEGRVVRRPYASGLMSAQWTIADVTL
                     VTLATGSIIFDGLSQTQTYFDLFVKNGLFGSTELRDSVTAVVFLGGLVAIVLVMARRL
                     GVAALGAGLLPVAVGYLVAHYLAYLLVDGQRIIAALNDPLLRGANLLPLDLAFWEPSL
                     FLPTAVLWSIQLAAVVGGHIVGAWAGHAQMARAEGGTTLVAQLPLAILMVALTTITLW
                     SLGQAVLVTPRN"
     gene            24596..25516
                     /locus_tag="VIF08_02740"
     CDS             24596..25516
                     /locus_tag="VIF08_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134931.1"
                     /translation="MRGATEQPPIERRFTLMTGTDPALAAGVLWWGPGDPQMQLGPHG
                     LVRAMRLASGPATLELAWAGQRVLAKAWGDGASEALALAPGLIGDLDEAPPLAAHHPI
                     VADLARRLPGLHLTRGAPVIEMLVNSIIGQKVTGLEARRSQRLLLARLGEPAPGPHRL
                     RLLPPPERLAGIPYWAFHPLGLERRRADTIRAAAAIAPRLEAIRAMSVDDARRRLESV
                     PGIGPWTAAETMRLALGDPDAVSVGDYNLPRQVCWVLASERDGDDARMLELLAPYAGQ
                     RARVVLLIERSGLRPARRAPRYAPRRIAGM"
     gene            25556..>25794
                     /locus_tag="VIF08_02745"
     CDS             25556..>25794
                     /locus_tag="VIF08_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134932.1"
                     /translation="MSNRVSRRQGVIYGPPRPEPEEGANSALIGRVLGIGMVALALAV
                     LAGAFVLVNRPPGPTPTRQVGPTASPPPGSPSALPS"
BASE COUNT         3951 a         8890 c         8732 g         4221 t
ORIGIN      
        1 gagtgggggg tcgaatcgac aggtcagtcg gaggtcgtgc tcaccagatc tgctggcggg
       61 ctatcggtcc gcaagtcgat cgcagttttt cacggccgga tgaatggctc gcttgacctg
      121 gacatcgtcg tcaccgctga ctcggccttc aggggaccgc tcgagctcga gtggaacctc
      181 aaccttctgg gtggcggcgc caacccggag gcgcactacc actgggccga ccaggaaatt
      241 cgccacgatg cgccggggtc ggtcgcggct ggcgtccagc tgtcatttgg taatcgctac
      301 gagggcgtcg ccatctccgt ccagctagag cccgcggcgc cacagcagtg gttcccagtc
      361 gaaaccgtgt cgaattcaga agccggcttc gagcgcgtct accagggctc gtgcctgacc
      421 cagcgctggc cgctggagct cgccgccgga gagtcgaaga gattcaccgt gacgatggcg
      481 ttcaagcaga cgcgcgaccg cgcgggggag gaagccggct agacccgcgt aggagagtcc
      541 atcccggccc ctgtgggatt cgatcccggc agatgtacct gtgggtcagc cttaagcgac
      601 gcgaccacgg ttgcgaggga gccgcatcgc gcctttcacg ctcccttagt cgcccctcgg
      661 cgaggaagag ggctcgacga gccacccaag gctgacgggc cagggccttt gctgggaatg
      721 actcaacgga cgggcgcggc ccagcgtgtc cagctgaacg tgaagtcgaa ggccaggccg
      781 cgatacatcg tgatggcgcc ggcgttatcg gcatcgaccg acagccagac caacttgtgg
      841 ccggtcgcga ggccggcgcg ggtagcgatc gcggtgatga gccggccgta gccgcgccgg
      901 cggtgccgct ccgcgacacc aacggcatgg agcgacgcga cgccgctgcc cggctccccg
      961 ggcacgagcc gggcacatgc gatcggctcg cccgccagac gcacgatgaa ggcgcgcgtg
     1021 tcaccggatt ccacgacgag agcgagcagc cccgcgctga cgtcgatccc gtcgggatcc
     1081 aacccgaagg cggcggattc gagcgtcgcg cactcgagcg cggtcgcggg cgtgactgcc
     1141 tcgatgcgca gaccagggtc gagatgcggc actaccgatg cgtggcgcgt gaacatcagg
     1201 cgctcgcttc cgagcggcac ccagcccgcg gcgcgcagcc gcgactcgag gtcgtgtggc
     1261 tcggtcaggc cctcggccac gacgacctgc ggccacgtgt tcgaggcacg catgcgcgcg
     1321 tcgactgcgg ccagccgggc gttgacgtca gcggccggcc agcgaataca cgacgcgaag
     1381 ttcaggccgg cgccgcgagc ggggtgggcc gcccacgcca cgccaagcgc gtcgtcacgt
     1441 tcgaccctcg ccccgagcag cgtgacccag cgcgtgcgct ccagttcgat ggcccgcaac
     1501 tcctctgcgg agggcgcggc gccagcgtct gactcgggcg catctacccg cggtcgtgca
     1561 ctttccggcc tggacggtgg tttcgttgcg cccatgtcgc tcacagaaac gccgcgcgaa
     1621 cctcgctgtc gaagcgctcg atgaggcgcg cgaccttgtc gggatccgtc tccgcgaaat
     1681 cgaccgcgac gtgagtcacg cccagggcgg cgaactcgcg cagcgtggcg atcatctggt
     1741 cgggcgtgcc gctcagcatg aagtacggca cgctgtgctc gccgaaggcg acccgaaaac
     1801 gcgccgagaa gcgcggctcg ggccggcccg cctcagccgc agcggcgcgc acgaccggca
     1861 cgcggggcgc gtagtggtcc gggccggtcg cggacgagtg gtagccgttg gccagctgac
     1921 cggcccggcg cagcgcggcc tcgtcccggc cgccgaccca tatctcgagc tcagcgccct
     1981 gcaccggcag cggcccgaag cgcacctcct cgaacgagtg aaagcgaccg tgaaatggcc
     2041 cctgaccgcc gccccacagg tggcgccaga tggcgatcgc ctcgttgaga taagcgccac
     2101 gttgcccaaa gcggtcggcg aggcccaggt gagcgaactc cttctcgtcc cagcccccgc
     2161 caatacccac gatcagccgc ccgcccgata gaacgtcgat ggtcgccagc tccttggcca
     2221 gctcgaccgc gttgcgcatc ggcacgacga gcacgctcac cccgacgcgc aacgtgggct
     2281 gacgcgcggc aacgtggacc accgtcgcga gcgcgtcgaa gatgtggaag taatcctcct
     2341 cgcgcgcgga cgtgccgacc agaaaatgat cggtgctgaa ggcggaatgc cagcccagcc
     2401 ggccactggt ttccgacgcc gcctcgatgc cctcgaccga cgcgcccggc cgataggagg
     2461 gcaggatcag tccgaagtcc acggtcaacc ggtctcagtc ggagccgaac agggcccgct
     2521 tgagccactt gatgggcggc gagccgtgcg agatcaccga ttcgaggtgg gcccgatagt
     2581 cgaagcccgg tgtctcaccc atacccccgg cgatgcgctg cggcggcacg acgtccgcgc
     2641 cggcgccggc cgccaccgcg gcccgacgac gtgccccctc ttcgagcgcc cacatctcga
     2701 gtgagcccag gtagtaagtc gtgagctggg tcgacgtcag ccgcgcgcgc agccacttgg
     2761 cagtcgcctc gtcgcgctcc tggaagccgc cgccgaccat gagctccatc gcctgctcct
     2821 cggtcatgcc ctcgccatgt atggcgacgt ccatcagcgc gttggtgatt gcgcgcaggt
     2881 agaacttcca gtggttgagc agcagggccg gttcgtgctc gccgtagccc atgtccatca
     2941 gcacctgggt cacgtacacc gcccagccct cggcgaacat gccgctcccg aagactgcgc
     3001 gcgccaggct gggcgagcgg ttggcataag cgccctggag gtagtggccc ggcacgccct
     3061 cgtgaatagc gaggaggcgc agcatgcggt cgttgtcctc gcgcaggtac gactcggtcg
     3121 cctccgcccc caggctatcg tccggcggcg tgatccagaa gtagcttgat tggcccttgt
     3181 cgaggactcc gggagcattc aggaacgccc ggccgaaggc gcgcatgaag accggcgtcc
     3241 aggtgatcat catcggctcg tcgggcaggc caatcacatc gttcgctcgg cagaagtcct
     3301 cgattcgacg tacctcggcg cggctgaagt cgagcagctc cgcgggctgg cggtgctcct
     3361 gcgcgaccgc gaacagggcg cgcctgatga gctcgtccgt gtccgccggc atctcctcgc
     3421 cgggcagcca ctcgggccag acgagccgcg ccaggcgcag catctcggcg cgcacgaggt
     3481 cgtagtcgcg gcgcgccctg gcgccgagct cacctggcgg caggtcgctc gacgtggtgt
     3541 ggcgcagctt cgcggcaaac aggtcagcac ccagccgccc ctcaccttcg gcgcgcggca
     3601 tgatctcgtc gcgcaacgct tccgcatagc gcgcgacggc cgcccgtgcc ggctctgcgg
     3661 ctgcgtggat ggccgccgcg acctctcgat cgtcgcccgc ccgcttctcc gcctcggcaa
     3721 tgccgtcgtc gatgagctcg gtgatgccat ccatctgcaa cagcgccgtt tcggcgtgca
     3781 ggcgcgacac cggccgcccc ggcaggccgg tcagggaagc cgccgccccc tcgaggaacg
     3841 ccggcaggtt gcgcagtcgg ccagcgaacg cagtaccgcg gtgcgcccac ggcgcgtact
     3901 cgcgcgcgag caggccgaag aggccgctgc cggcctgcca gacgtagctc agcgcatccc
     3961 acgcgtgctc gcgcagctgc tcgtcctcga agatcatgcc ctcgatcgcc tcggcgacga
     4021 tgccgcggtc gatctgctcg tcggcgctca gacccgacgc cgcgagcgcc tcaaaccggc
     4081 cggcgtgatc acggagcatg gccagccggg cggagcgtcc ggcttcggtc atgttcggcc
     4141 agcggtcgtc gtgagcgtgg aagccgatct gcgtcgccca caccggctgc gcggcaaaga
     4201 agtcgtccag gaatgccttc agcgcatggt cgaaggcagt cacgggtgtc ggtcggttgt
     4261 cgggtggaag ggacatgtcc ggcatcctag cgacggagcc gagctgtagc atcagcccat
     4321 gcttctcgac ctgctgttgc gcgtggccat aaatgccata gccctgatcc tggcggtgca
     4381 gttcgtgccc aatgttcggg ccccctctga catcctgaag ctcgtcctgc tggccgtggt
     4441 cttcggcctg atcaacgcct acctgcgccc catcgtgaag gcgctcagcc tgccgctgac
     4501 catcgtgacg ttcggcctcg tcagcctggt tatcaacgtg gcgatggtcc tcgttgcggc
     4561 ggcgatcagt gacaacctga atttgggctt cgcgctcggt ggctggccgc tgaacgatcc
     4621 atcgatcggc atcgacacgc tcattgccgc gttcctcgtg tcgctgctga tcagcatcgt
     4681 cgccgcagtg gtggcgctcg ttcgccgact gaccccgggt atctgactca gcccctctac
     4741 tcaggagagg ccgtcccggg ccttgccgta acctcttgac cgtgagtggg acgtcgtcga
     4801 gccagaggga cgcgtttgcc gcgcgccttg ccaaggcgcc gctgctgctc gacggtgcca
     4861 tgggcacgct cctctactcg cgcggcgtac ctcagcgggc gagccttgac cagctcgtcg
     4921 aggtgcggcc cgacctgatc ggcgcgatcc atcgcgagta catcgaggcc ggcgccgacg
     4981 cgatcgagac ccattcgttc ggcgcgaacc ggctgcgcct ggcgcccttc ggcctggccg
     5041 acaaggccgc gcttttcaac cgccgcgcgg cgcagatcgc acgcgaggca agagatgtcg
     5101 ccggcaagcg cgatgtgctc gtggccggct cgatcgggcc gctcgaggca ccgttgcacg
     5161 gccccgacgc cgcgaaggaa gaggcgatca gggccgcgtt cggggagcag atcgagggcc
     5221 tgctggaagg cggcgtcgat ttcctgctgt tcgagacatt cagcgacctg cgcgagctgc
     5281 tgatcggcat cagcgcggcg aaggcactgt ccgacctgcc gatcgtcgcc cagatgacgt
     5341 ttggcgagga gctgctcgcg gtggatggca ccagccccca gaccgccgcc acggcactcg
     5401 cagcggcagg cgtcgacgcg gtgggcgtca attgcggcgt cggcccgcag atctgcctcg
     5461 acgcgctgac ccagatgggc cgcccggcag ccggcctggc gcgctccatc ctgcccaacg
     5521 ccggcctgcc gcagcgcgtc gaagggcagt tcgtctacgc cgcggagccg gcctacttcg
     5581 cggccgaggt gccgcgcttc cttgatgccg gcgcacgtat cgtcggtggg tgctgcggca
     5641 ccacgcccga gcacatccgc cacatgcgcc aggccctcga cgcctacaac gcgcacaagc
     5701 agtcagcgac ggcatccgtg gaggcgcccg agatcggcgg cacgcctcgg acgcccgtcg
     5761 ccgagcgacc cagcgggtcc atcgccgagc cgccgccacc gacccgcctc gcgcagctgc
     5821 tgacgcagga cacgttcatc ctcagcgtcg agatcgaccc gccgcgctcc gtgcgcatcg
     5881 aacgcacgat cgaggccgcg cggctcttgc aggatgctgg cgttgacgtt gtcaacatca
     5941 gcgacagcgc catggcacgc gtgcgcatgg gcgccctcgc ggtggcgttt ggcgtgcaac
     6001 atgatcttga tctcgagtgc ctggtccatt tcacgacgcg cgaccggaac ctgatggcgc
     6061 tcgagtcgga actgctcggc gcgcacgccc ttggcgtgag aaacatcctg gcgctgaccg
     6121 gcgatccgcc gcgcgtcggc gactacccga ccggcacggg tatctgggat gtcgactctg
     6181 tcgggctgat cggcatcctc gcgcggctca accgcggtga ggaccaggcc ggcaagccaa
     6241 ttggcagccc ggcgggtttc accgtcgcct gcgcgctcga tcccaccgcc gacaacctcg
     6301 gacgcgagat cgagcgcctg gccgcgaaga tggacgccgg cgccaacgtg gtcatgacgc
     6361 agccgatcta ctcgcgcgac cagtgggatc gcttcatgga gttcgcagcc gcgcgctggc
     6421 cgagcggcct gcccgtgccg gtactgctgg gcgtgctgcc gctccacacc gcgcgccacg
     6481 ccgacttcct gcacaacgaa gtgcccggca tcactatccc gaaagaggtg cgcgccgcta
     6541 tgcatgccgc cggcgagcgc ggcgcggagg tcgggctcga gatggcccat cggctgctcg
     6601 actcgctcgc gccgctcgtc cacggcacgt acatcatgcc cagctttggt cgctacgagc
     6661 tcgcggccga gctcgtccgg cgcatccgtt ccgacactcg catcgtcacc agcgcgagga
     6721 gccccgcatg acccgaaccc ggtttgcccg ccttgtccgc tcggcgcttg ccgccggctg
     6781 gctgctgctc attggtggga gcgctgtgct ggcggccgat ccacccctgg ccgggccgcc
     6841 ctttccggca ccccaagccg acgtcgtggt ctacgactac gcggacatcc tctccccggc
     6901 caccgaggcg cgcgcaaccc agagcatcac cgatatcgag caccgcaccg gcgctgagat
     6961 tgtcgtctac acgcagtaca agcccgggtc ggacgaggac tcgaccgagc aggacgcaat
     7021 cgacctgatc gaccagtggg gcgtcggccg cgcgggcttc gacgatgggc tggcgatcat
     7081 gtggaacacc ggccgcctgg aatgcatgcc cggcgtgtcg ggcaacggcc aggtccagct
     7141 ctacgcggcg cccggttacc gcgcggcgta tctgtcgaac ggagagcgcc agtcgatctt
     7201 cgataacgac atgctgccct acctgcgcca gtgcgacgaa gacagcgcat tgctcgcggc
     7261 actcgacaag atcgacgccg cggccactcc ggagcacgcc cagatgctcg atcgggcgcg
     7321 catcatcgac gccgcgatcg gcctgatcct gggcccgggc atcttcatcc tgctcgtcgg
     7381 ctgggtcagc atctcatggc tgcgctacgg caaggatccg gtgtatctcg acgatccgtc
     7441 ggtgctgatg cctgcgccgc cgcccgatct gacagccgcc tcgggcgcag tgatctggga
     7501 tggccgctcg tcacggcgtg cgctcacgac ggcgatgctt gacctggcca gccgcggtga
     7561 gatcgggttc aagcccgaga gcgagctgct cggcctgcgc accaaggcgg gcatccagat
     7621 caatgagacg tacgacgccg acgatccatt gatcgaccgc aaccgacgca agccactgtc
     7681 gaaggcggag cagtacgcgc tcgaacggct gcgcggcatc gcctcgcccc tcaccggcaa
     7741 ctacatcggc gcttcggacc tgctgaagtt cggcaagtac gtcagcggct tcgacaagca
     7801 gatcgaagcg cacgtcgtga gtaagggctg gttccacgag gcgccggcca aggcgaccga
     7861 acgttgggct tcgcgcgcga tcctgatcct gatcgcgggc ttcgtcggcc tcatcatcgg
     7921 cgtcaacatc cccagcggcg gcgtggtcgt attcaccgtc gccttgatgg ccgcggcctt
     7981 cgtcatgctg ttcatcgcac gctcgatgcc ggcgcgcacg atggccgggg cgatgatcta
     8041 cgcgatgctt gccgcgtatc gaagaaccat ggaaaagacg atggcccagg cgcgctcgat
     8101 gaacaccgtc gtggaggagc cggcgctggc ctggatggag acacccgatc aggccgttgc
     8161 ctggggcgtc gccctggggc tgcagcgcga cgtcgaggag gtcctggcac gcagcgccga
     8221 ggacgcgcag gccggcgtca ccacctacca cccctacctg cccgcgtggt acggcggggg
     8281 cttgacctcc ggtcagggcg ggcagggcgg gttcgcgccg ggcctgatgt cctcgtcggc
     8341 gatacccaac ttcgggggca tgatggccgc gctgtcatcg atcggcaact cgcccagctc
     8401 atcgggcagc ggcggctcgg gcgggttcag cggtggcggc agcggtggtg gcggcggcgg
     8461 tgcgggaggc ggctactgag cgctgcctgc gtagcaaagt agactcagga ccgtatggga
     8521 gccactgaaa caatggagca gttcttcgag ctgctcaata acggccagcg cgatgacgcg
     8581 ctcaagctga tggacgagaa gatcgagatg cgcgtccacg tgggcgacaa cgcgcgcaca
     8641 ctgcgcggcg tcgagcaggt gggcggctgg ttcctgcgcg ccgacaaggg catgcgcatg
     8701 attccggcca acgagcgcga tatgggcgtg aacctcgaga tcgacctgat ggtcatgcgc
     8761 cccggcgtcc agtcgcagca tctcgacgcg acattccgcg tcgaggccgg caagatcact
     8821 gcgatcaact tcgcgccgcg ctgagcggcg gcgccgtggt ccgtcgcagg cggcggctag
     8881 gaccctgagc tcggcggtat gacgatcacc tggcccggca cgatgtggtc ggggtcagtg
     8941 atctccgggt tcgcggccat gatgtcggcc acggtgacgt gaaaacgctt ggcgatcttg
     9001 cccagcgtgt cgccgttctt gattcggtac agccgcggcg tggcatccgg ttccgccgtg
     9061 gcggtcgggg cgacggttgg cccgatcgag gtcacggggg tggtgctcgc cgcgggcacg
     9121 ctgggtgagg tcaggaaggt cgggccgagc gtcgtggccg gcgttgccgt cgcgcgaggg
     9181 ctgccggcaa gcatgcccgg caggaagatc acgcccgcga cgcccagcgc cagaatggcg
     9241 acaacggcga tggcggtcat cagcccactg atgcgatcgc caccacggcc gccatcgtcg
     9301 tcacggccgc gccggtgagc gtgctcgtcg aggtcggcgc cgtaacggtc ggtcaagtcc
     9361 caagatggga cgacatcctc gcgcgacacg gtttcgcgca actggacatc gggtcgcgac
     9421 gcgcgcggtt gcgcaccggg cgtgcgctct gtccggcccg ccaggaagcc cggcaccggc
     9481 gcgtcgtcga agtcgtcatc cggcgtctcg tccgctggcc ctgcggatgc gccagcgagg
     9541 aaggccgggg caacggggct ggcgctgacc gtcgaccagg cgctggggaa ttcctgctca
     9601 gccggcgacg caggctcaac cggcgcgtca ctttcgaaga cggccagctg cgccggggca
     9661 tcgaccgcag ccgccgggct gaacgcctcg acgggccacg cctcgcccgg cgcgggcacg
     9721 atcggctcca ccacgggcag cggcgcctgc gccgccgcgg cctgcacctc cgcgactgtc
     9781 ggcacgggtg cctgggactg ccctggccgg ccctcgtagc ccgctggggt gggcacgggt
     9841 cgcgccgcgg cacgcatcgc ccagccctgg aagatgggac acgccgcgaa ctcagtcgag
     9901 aggcagtacg cctcctggtg cgcgatcgcg cgtggctgtg gggtcggctc cgcgaagcag
     9961 cgatgccggt agtcgggctt gtcggagcgc cgatcccgat cgagctcgag agcgatgaat
    10021 gggcacgcct gcgggccttc cccgaacagg tctcccctct gactcatggt gagtcggatt
    10081 ctacgcggcg caccagaggg aatcaggccg ctgcgggcac ccgcgctgcg tcgtcggtgt
    10141 cggtctcaag ggctgattcg aggggcatct gctgcttctc agggccggcg tggatcacca
    10201 tgcccgcgcc gaccgtatcg aaggtcgcct catcgatgag gatgaagccg ccggtcgtgc
    10261 gcgacaggtc atacgggtcg agcagcagcg gcgcgctcgt gcgcacgtgg atccggccga
    10321 tctcgttgag gcccaggctg tcggcggcct gatcgcggtg gagcgagttg acgtcgatgc
    10381 ggtagcgcag gtcgtcgacg accgccttga ccgtgcgcgt ggtgtgcttg agcaggtacc
    10441 gaccgcccgg cttgagctcc gattcggaca tccagcacat catcgcgtcg agctcattgg
    10501 cgacggtggg ctggttgtgc ggccggcaga tcatgtcgcc gcggctgatg tcgatgtcgt
    10561 cttcgagcag catcgacacg ctcatcggcg gaccggcagc ttccagttcg ccgtccatgg
    10621 tctcgatgcg cgcgatgcgc gaggtcttgc ccgacggcag gatcgtgacc tggtcgccct
    10681 tgcggaagac cccagcggcg atctggccgg catagccacg gtagtcatgg tgctcgtcgg
    10741 tctggggccg gatgatccac tgcacgggga agcgcgcgtc gatcaggttc cggtcggagc
    10801 cgatgtagac gctctcgagg tggtagagca aggacgggcc ctggtaccac ggcatggaca
    10861 gcgagcgcgt caccacgttg tcgccgtgca gcgcgctgat cggtatgaac tggatgtcat
    10921 gcacgtcgag cttctgcgcc cacgtcgcga actcctcgcg gatcgcgttg aaggtctctt
    10981 ccgagtagct caccaggtcc atcttgttga ccgcgacgac cagatgcggg atgcccagga
    11041 gggtcgcgat gaacgcgtgg cggcgcgatt gctcgaggac gccgtggcgc gcatcgatca
    11101 gcacgatggc gaggtccgcg gtggatgagc cggtgaccat gttgcgcgtg tactgaacgt
    11161 ggccgggcgt gtcggcgatg atgaacttgc ggcgcggggt tgcgaagtag cgatacgcga
    11221 cgtcgatcgt gatgccctgt tcgcgctcgg cgcgcaggcc gtcggtcagg agcgccaggt
    11281 caacctgacc gtagccgcgc cggcggctgg tcttttcgat ggcggcgagc tggtcctcga
    11341 agatgccctt cgagtcgtac agcaggcgcc cgatgagagt cgacttaccg tcgtcgaccg
    11401 atccggccgt ggagaaccgc agtagatcca tgtcttagaa atacccctcg cgcttgcggt
    11461 cttccatcga cgtctcgctg aaggcgtcgt cagcccgggt ggcgccgcgc tcggtgatgc
    11521 gcgcggccgc gacctccagg acgatgtctt ccaggctcgc ggcgcgcgag tcgacggcac
    11581 cggtgcacgt catgtcaccc acggtgcggt agcgcacggt catgcgctgc acctcttcgc
    11641 cgtccttggg tgggaggaag tcactcacgg cgagccacat gctgtcgcgc cggaacacgt
    11701 cgcgctcgtg gctgaagtag atagagggaa cctcgatctc ctcgcgctgg atgtactgcc
    11761 agacgtccat ctcggtccag ttggagatgg ggaagacgcg catgtgctgg cccttgttga
    11821 gccggccgtt gtagaggctc cacagctcgg gccgctgggc cttgggctcc cactggccga
    11881 actcatcgcg cagcgagaac acgcgctcct tggcgcgcgc cttctcctcg tcgcgtcgcg
    11941 cgcctccgaa aacggcgtcg aactggtgct ccaggatcgc gtcgagcagg gtcgtcgtct
    12001 gcagccggtt gcgcgacggg ttgcctggct cctcgcgcac gcggcccttg tcgatcgact
    12061 cctggaccga tgcgacgatc agccgctcac ccaagcgctc gatggtccgg tcgcggtagg
    12121 cgaggacctc gggaaagttg tggccggtgt cgatgtgcat caccgggaac gggaacttcg
    12181 ccgggcggaa ggccttctcg gccaggcgca gcatcacgat cgagtccttg ccgcccgaaa
    12241 acagcagcac gggcctttcg aactcggccg cgacctcgcg catgatgaac actgactcgg
    12301 cctcgagggc gtcgaggtgg gtgatgttgg cgcgcgccat ggcgtcaggc tgtgcttcga
    12361 gagggatcac gggaggtcaa tcaccacaat aacgatagga attggcaaac ttgtgaagtc
    12421 accgtagtat acccgccaat gtccgacgca caagccccac cgcgttcggc cgattcgtcg
    12481 cgcctcgaca tctgggcgga gcgcatcggc gtgtcgcggc gcacgctcat cgccggcatc
    12541 gtgctgccga tcatcagcct gatcgccatc tgtgtgacct tcgccctcgc cccggccgac
    12601 cagcgcggcc tgatcttctt cagcacgctg gtggcccttg cagccggcct ctcatcgacg
    12661 gccatcggcg tctacggcgg ggtactcgtg cccggcctgc tgctgctggg cgttgacgcg
    12721 cggttcgctg cggccgcttc cctgttcctg caggtgctgg tcatcccgct cgcggcgagc
    12781 tcgcactacc gcatgggcaa cttctcgcgc aacgtggcgc tgccgctgct catcggcggc
    12841 gtgatcggcg cgttcatcgg cccgttcttc gccgccgcga tccccaagga atggatcgtg
    12901 cgcttcgtgg cggcgatgat cgtgttcgtc ggcatcatcg tcctcgcgac gctccacctc
    12961 accggcctgg gcaaggtgcg cgccgacgac gacatcccgc aggcacaggt cggcggcatt
    13021 ggcctgctgg ccgggttctc atcgggcgtg tccggcgccg gctggggccc gatcggcgtg
    13081 aagctgctga tcctcagtcg gatcgatccc cgccaggcga tcggctcatc gctgttcgcg
    13141 cggatcttca tggccgcggc ggcggttgtc gggttcttcg tcgcccagac ggcgttcaac
    13201 aacgccacgg ccaactggtg gatcgtcgtg ccgctcttcg cgggctcggt cgcgccgatg
    13261 atcgccggcg ccatgctggt ttcgcgactg ggccgcgaac gggcaactgt cgccatcacc
    13321 ctgctgtcga tcgcgctggc cttgccgagc ctcctctggg gccactagcc ccgcgatggc
    13381 ccgattgccc acgctggcgc aaaatggtcc acctgaagcc tgaaggagac gactgaaatg
    13441 ttccggatcc tgagtgccct cgcgccgagc gcaaccgccc acgcccattg cgacatcccg
    13501 tgcggcgtct atgacccgca gcaggcgcgc atcgaggctg agtcgtgcta ccgcatcatc
    13561 gagaagtacg cggcgtcctc cgatgagctg ttccgcgcgc gctgcatcgt ggtcaaggaa
    13621 gagcgcgccg agctggccaa gcaccacatc gacgtcatgt ggcacgacta cttcaagccc
    13681 gagcacgtcg agaagttccc gaccctgcac gagacttgct ggaaggcggc taagcaggcg
    13741 tcgctcgtca agcgctcgct cgacatagcg gaggcacaga agctgctcga cctgatcgac
    13801 aagatcgatc acatgtggcg tgagacgggc ggcccggccg ccactcgcat gccggcggcc
    13861 gcctcagcct gatccgccga ctcgtccgct cgtggcgcca tcgtgtcgtc gtcgacggac
    13921 actcgatgga gccaacgttt cacgacggcg attggctgct cgtcgaccct gacgcgcggc
    13981 ccgggctggg cgacctcgcc gtcgcccgcg tcgcggagcg gctcatcctg aagcgtgtcg
    14041 tggacatcga cgagggcggc cgctacaccc tcgctgccga caatccggtc catgcgggcc
    14101 agcgcatcgg cccgcttgag cccagcctgg tgatcggccg tccctggttc cgctacggcc
    14161 caaggcgcag gtacggccca ctcgattgac ggccgcaggc gggctgcgca taatgctgaa
    14221 tgctgcgtta actatttaga tggactgaac aacccttgcc tgtcgctgcc ccagaagaca
    14281 tcgtcgagca gatcctcgat cagctggagc ccttggtcgc ccaccagcgc cgcgccgttg
    14341 ccaaggaagg ctgcttgcgc gcgatttcct cgacgcagct tcacgtcctc ttcatgctgc
    14401 tgagcgacgg cccgacgacc atgagccgcc tcgccgaatc actcaacgtg tcgctaccga
    14461 acgtgaccgg catcgtcgat cgcatggtcg agcacggcct cgtggagcgc ggccgcgccg
    14521 acgaccggcg tgtcgtcacg gtgctcgcga ctgacgcggg acggcacacg gtcgaggaga
    14581 tcgacctgct gcgccggcgc cggctcacgg cactgctcgc gcaactggat ccggaccagc
    14641 agcggcgcgc gctggaaacc ttcactgagc tgcgcctcgc cgctgagcgc ctcgaccgcc
    14701 aaggagcccc cgcctgagaa tggaagccac tcccccaact accgaaggcg cgtccgccac
    14761 gccggcagtc cagatcgacc accgggtgcg caccgagatc ctgatcggca tcctgctcgc
    14821 gctcttcctg tccgcgctcg accagaccat cgtcggcacg gcgatgccca ggatcctgac
    14881 cgacctgcac gggatcgatc tctacacctg ggtggtcacg atctacctgc tgacctcgac
    14941 catcaccggg cccatctacg gcaagctgtc ggaccagttc ggacgcaaga acctgctcat
    15001 attcggcgtg gtcgtgttcc tggtcggctc ggcgctgtcg ggtctaagcc aggacatgat
    15061 ccagctgatc gtcttccgcg gcattcaagg catcggcgcc ggagcgatct tcccgatcgc
    15121 gctggccgtc atcggcgatc tgttcacgcc gcgtgagcgc ggccggtacc agggcctgtt
    15181 cggggccgtg ttcggcatct cggccctggt cggcccggcg ctgggtggct tcctgaccga
    15241 cagcatcagc tggcactggg tgttctacgt caacatcccg atcggcgccg tcgcgctgtt
    15301 catcatctgg cgcctgctgc cgccgctgca cacggcgggc gtcacgcgca agatcgacta
    15361 cctgggcgtt gcggtcttca cggccgcgct ggtgcccatc ctgatcggcc tgaccaatgc
    15421 ccagcagcac gattggaccg atccgctcgt cggcggcctc atcctgatcg gccttgcgct
    15481 gggcggcctc ttcctgtgga tcgagacgcg cgctgccgag ccgatcgtgc cccttgggct
    15541 gttccgcaac cgcacctacg ccatttcgat cgtggccacg ttcctggcga gctttggctt
    15601 cttcggcgcg gtcctgttcc tgccgctgtg gtaccaggtc gtcaacggtt ccacggcgac
    15661 tgaatccggt taccagctgc tgccgctgct gggcggcctg atcatcagct cgatcgcgtc
    15721 gggccagatc gtgagccgga gcggccgcta taagtggctg accgtgggcg cgctggttgt
    15781 cctgtcgatc ggccttctgc tgctgacaaa tcttcgagct gagaccccac cgccgatgct
    15841 ctggctgtgg cagttcgtga ccggcctcgg cgtgggcccg acgttcgccg tgttcacgat
    15901 catcgtccag aacgcggtgc cgtggaacca gctcggcgtc gccaccagca acctgacgtt
    15961 cttccgccag atcggcggca cggtcgggct atcactggcg tggaccatct tcggttcgac
    16021 gctgcgcacg gaagcgccga cgcaggtgac ggcccagctc gtcaatgccg gggtgccgca
    16081 acagcagatc acgctcttca ccagcaactt caccctctcc gggcagcagc tcaacgactt
    16141 cgggtcggtg ggcgacatgg gcgcgcgcat ccttgcccag ctgcccgagc aggcccgcca
    16201 gttcgttgag ccgctgatcg gctacatagt cgcgggcatc caccaggcgc tatcgatcgc
    16261 gatcgccaac tcgatgtggc tgggcgtggg cgcggccctg attgccgtgt tcgtcgccgc
    16321 actgatgcca gagctgccac tccggcgcga ccacaaccca gacagcgccg ccctcaaggc
    16381 tgcaccgcct actccttaga ggaacacctc gcccagcagc gtgaccatct cgaggtcgcg
    16441 cggcaggaac gtctggagca tcacgcgctc cacgcccacg gcgccgagct cggccacgcg
    16501 ggcgcgtgcc tgctcgggcg tgccaaagat ccagcgccgg cgacgctcgt cgagccagcg
    16561 ctcaccctgg agaccgccgc tgttgacgct cgcgagcagc gcctgggcgc gccgctcgac
    16621 gtcggcctcg tcgcgaccga cgagcacgcc ggtcatcgct gagcgcgtca cgtcgcgcgg
    16681 gtcgcggccg gtcgcgcggc acgcctcatc cacgcgcgcg tagcccgctg atgccgaacc
    16741 gggcgtggcc gaggtgatgt tgatctcgtc ggcgtagcgc gccaccagcg cggccatgcg
    16801 cgccttggcc tcgccaccga ctatcaggtt gggatgcttt cggcccaccg gcgccggcgg
    16861 ccgcggcctg aagagcgagt cgctcacctg ccagtgcgcg ccggtgtaac tccagccgtc
    16921 cggctccgtc cacaggccgt ggacgatggc cagctgctcc tcgagcatgt cgaagcgctc
    16981 gcgcgtgtcc ggaaaggcca ggccctgctg gtggtgctcg ggctcgttcc agccggcacc
    17041 caggccgacc tcgacgcggc cgccgctcat ctcggccacc gtcgcgctga ccttggcgag
    17101 gttgcccggg gcgcggaatg tcaccggcga gacgagcacg cccaggttga tgcgcgtcgt
    17161 ctcgcgcgcc aggccggcgg tggtcgccca ggcgtccgtg gtgggcagac cggcaccgcc
    17221 ggggaagctg gcgtagtgat cggagcgaaa gaacgcctcg aacccggccg cctccgcggc
    17281 gcgtgcgagg gccaggatct ccgtatagct cagcccctgc tgaggctccg tcatcagcgc
    17341 gaagcgcatc tgctctccag agtcagccgc cggcacccgc gtcggggcgc cggccttcgc
    17401 gctacatggt cacatggacg gcggccaagg acctgatggc ccggcaccgg gctgcgcgac
    17461 cgggtgtagc cgccgccagc gcagccacag ccgattgacg acccagatcc cgatcacgac
    17521 cggacccacg accggcagga cgacgatgac cagccagacg agcaggctgg ccaggccctg
    17581 accgatccga accagcgatg ccaacgcgcc atcgatctcg cgacccaggt cccagccggt
    17641 ggatgcctgg gtcacctccg ccaccggggg cgggttgaag gcgacgttga gcgtcgcgag
    17701 ggccgcctgg ttggtgagca ggtcacgctg ggcggtcatg ctctcgatat cgccctggac
    17761 ctgcgtcagc tcagcctgca ccttgagcac gtcggtgatc gtgttggcgc gggccatgat
    17821 gccctgcagg gcagcctcgg tcgcgcgcaa gttggccaag cgtgcctcca ggtcgacgac
    17881 ctgcgcggtc acgtcatcag atgatgtctc ttcattggcg acgcgcgtcg ccaggccctt
    17941 caggccgctc agcgcctcgc tccagcgagc ggccgggatg cggtacgtga cgtacgccga
    18001 cttgccattg ccgctgtcgc tggcacgtga gcccgcgacg tggccgccga ggcccttgac
    18061 cagcgcctcg gcctggctga ccgacgtgtc aacatcggcc acctccagat cgagcgagcc
    18121 gttgtagacg atgagctgcg gctgttcggg aatgaagttg ttgccgttcg ggaacgggtt
    18181 attgccgttc cccggcgccg cggtgggtgc tggttgatag ccgttgccgt caccggtgcg
    18241 gtctccaccc ggggtgggcg ctgcggctgc gccgctgcac gcggccacca acccgaccag
    18301 cagcacggcc gccgccgcga gcacgagacg tcgaccacgc atcttcaact cctgtccccc
    18361 caacgagcct gcctggctcc cagcctcggg ctgagtttcc cacccgcagg tttcgacgcg
    18421 tagtgcagtc gattaagttc ccgatgcgct gccagaatgg ccgcgtgacc actgatcccg
    18481 acgcgcgcgt catgccgatc cccgctgggc tggtcgcgtt cggcgccgcc acggccgagt
    18541 tcgtcgggct ggcccaggtg gccgacctgc ccgcgggcac gatgctgcgc gtcacgcgcg
    18601 gcgaccacga cctgctgctg gtgaacggtc agggtggcat ctgcgcgatc gacgatcgct
    18661 gcccgcacat ggccgcgccg ctgtcactag gaagcctcga ggggtgcgtg atcggctgtc
    18721 cgctgcacaa gggcaagttc gacctgtgcg acggcgggat cgtccagttc ccgacgaccg
    18781 gtggcctcga ccctgacggc ggctaccacg ccccctggac gccggccgat gctccgccca
    18841 agcccgagcc gaccgacctg aaggccatgg cccgtgcggc gacccgcgtg cgccggctgc
    18901 gctactaccc ggtacacatc gtcgacggca ggatcgaggt ccggctgccc ggctgagcgg
    18961 acgccggact acccgctgag cgtggttgtc cgccgaaacg agcgcgaatg gcgggcagga
    19021 ttcgcatagg tggggtcttg tcgggtaaag ttcgagccag ccgagaagtc gggcgagagg
    19081 gtagccgtag cctccgaacc gtcgtcaccg gagagccgtg cgtggatttc cgtacaacca
    19141 ggacaattcg cagtgcgata gagattcgcg cgccgctcga cgtcgtgtgg cgcgtgctga
    19201 ctgatttcga cgcctatccg gcgtggaatc cgcacgtccg gcgcttggtc ggcaagccgc
    19261 aggccggtcg ccggatcggc attcacacgc gcccacccgg cggccgcacc gtcgtgatgc
    19321 gcccaatcat ccttgactgg tcgccgccgc acgagctgcg ctggcgcgcc acgttcgtca
    19381 gccggcggct gttctccggt gagcacggct ttcggctgga ggaggtcgcg ccggatcggg
    19441 tccgctttgc ccaggacgag accttctccg ggctgctggt gccgctgtac gcgcggctgc
    19501 gtctgccctc cacgcggctg gggtttgccc agatgaacga ggcactgcgt acgcgcgccg
    19561 aggcagaggc cgcggcggct gcgccggccg cttcagttgg ggccggcgcg tgacccgcca
    19621 tttccgccca gggacggccc aatggtcccg ttttgctgaa cccatcggcc aatcgcaaac
    19681 cttttctggc gtcggtacct tgcgccacat gacacacccg cgtcccagcg cgtgggcgct
    19741 catctgcgtc ctcgtggccg gcctcctcgg ctcagccggt actggcgtga ccctggctgt
    19801 cgacaaaccg acgcaatgca ccggctggac cgacgagttc cacccgccga ccgagatccg
    19861 cgtttaccgc aacgatggcc cgaacgcggg ccacgtcgaa gttgtgccgt tccgggacta
    19921 cgtcggcacg gtcctcgccg ccgagtactc gaccgctggc ccgaagggcg cgatctggtt
    19981 ccgcatgggc gccgtgtcag tcaagcagta cggctggtac tacacgatgc actaccgcgg
    20041 cggcaaggtg acgaactaca acgaggacgg caccgtcgcc tcctacgact gctgggacgt
    20101 ccgcgattcg acgatcgatc agctctacaa gccggtgaaa ctggtcgatg gccagtgggt
    20161 gccgcgcaac gtgccctccg cggcgaacct gtcggcgatt gatgaaacct ggcacatctc
    20221 gctgcgcaaa tggatggccg acaagaacaa gagccggttc ttcctcaccg gctatcgatc
    20281 gggcaagaag aacccctgcg gcgcggatgc gaagggcttc aggatcatgc agaagagcct
    20341 gggcgactgc atcgtcaagg gcctcgtcta cgaggagacg acgcgcgcct actacgaacc
    20401 caacgtgctc atcgtcgatc cccgcgagcg cgacatcctc gacgacagca acacctactt
    20461 cggtgacgtg ggagcgctca gcctgggtgc gaacaacacc ggatggcgcg tatacgcggg
    20521 tctcgctgac ggctttgctg acccggtgac gggcacattc gcgaacatca cgtcgggcaa
    20581 cttcggcggt cacggcatgg gcgacgtcac cggcgatggc cgcaatgacc tggtgatcct
    20641 gaccctgggg gcgaccaaca agctccgcct cgccgtggcc aacggcattg gctacgctga
    20701 cccgacttcg caggacctgc ctggcggtgc cccgaccgcc cgcatgctcg tcgcggactt
    20761 caacggtgac ctgctggcag acgtcgggct gctgcgctca acgccggtcc agccgctgcc
    20821 cgatgagccc gcgacgatgg tcgtcatgcg cggcaatgca aacggcacgt tcagcgcccc
    20881 ggtcgattgg tggcgcggcg ttcttgacct ggcaactcag caggtcgccg cgggtgacgt
    20941 caacggcgac ggtaaggcag acctcgtcat gagcgacggc gtcgccggag tcacattctc
    21001 cgtggcgccc tcgttcgcat cgtgtgccga cctcacagtg tggggtccct gccccagcgt
    21061 ggcggggatc ggtctcgacg tggcgaccca gtggctcaac aaggcgagct gggtcgcggc
    21121 ggaagtcaaa tggacgatga ccgacttcaa tcgcgatggc cgcactgacg tggtggctgt
    21181 gctgaaggac ggtagcggta tcgatgtggt cggtgcccgg gccatcaagg gcggcggctt
    21241 cgccgatacg acgatgatgc tcgggatcaa cagtcttgcc tttggcgatg tcacgccggt
    21301 cggtagcgaa atcaacccgg acgggctggg cgatctggtt ttgctaaaga agaacggcgc
    21361 caacacgaca gtgcaatggc tgcgcgcgtc gcaagccgtc ggctcgccgg cagtgacata
    21421 cgtcacgacc actgggttga acgacaccgg cttgccgtgg gggccggcga cgaccaaggc
    21481 ctactgatcg gcggcgcgca accaggcgcg ccactcatcc tccgccaggc caagggacaa
    21541 ccgattcccg gctcgcacaa ggggcaaccg aatcaggcgc tgatcgcgca ctagcttctc
    21601 gaatgccgcg tcggccgaca tgctcaggta gcccagtcct gcgtcctggt atgcacgact
    21661 cgcggtgtcg aacaggttcg caggtccgaa tcgagcactg aaacggcgca actccgtcgg
    21721 cgccatcggc ttcgctccca ggtcaacgaa attgacctgc acgcggcgat cgcgaaagaa
    21781 gcgcagcgcc ttctgtgtgt cgcgcgagtc gcgccggccg aagacctgaa cgacgaccgg
    21841 cgggacctgg gcgggcaggc ctacttggcc gtcttgcgcc ggccgcgctt cgagcggcgc
    21901 tcaggggtcg tcaggccgat cagccagccc ttgtcggact tgcgcacgac aatgtccttc
    21961 ctggcctctt cggcggcgcg catgatgcgc tggcgcatgg tcagcggctt ctcgtcgccg
    22021 cccagccgga tctcgaagac gtccttggcg gacgcgatct ggccgatcgc cttgacgagc
    22081 tggcggtgct gcttcaggcg agccagcgcc ttggggctca gtgctcgcgg tcgggcggcg
    22141 gcctttggct tgtctagcat tgccacatca actttttgaa ttctcacgat ggcctcgctg
    22201 gtattgcgga gtaaggcccc caacggcgaa tcatgttaac cctgcctcaa ttggagcgct
    22261 caagatgaag atcggcgtaa tcgttccgca gggatggact ggtgaatatc gcggccggat
    22321 tgcagttgat gcatgggcgc gaacccggtc ggtcgccagg cagagcgagg ccctgggatt
    22381 tgaatcgatc tggttgttcg atcactttca cacggtgccg cgaccaaccc ttgaaattac
    22441 attcgaggca ttcacgtcgc tcgccgccct ggcggcggaa acatcgcgcg tgcgcctggg
    22501 acatgttgtc ctgtgcgccg cgtatcgcaa tgcggcgctg acaacgaaaa tgatctcgac
    22561 gctcgacgcc atcagcgccg gtcgagcgga gcttgggctg ggcgcgggct ggaaacgcga
    22621 cgaatggcgt gcctatggtt acggcttccc ggacgcgcca gaacgcctcg cgatcctcaa
    22681 ggacacgctc gaggttgcgc gggccatgat gaccgacgac cgcgccacct acgccggcaa
    22741 gcatctgcac atcgccggcg ccatcaaccg gccacggccc gtgcaacagc cgcgaatccc
    22801 gatcatggtc ggcggcaacg gacccaacgt cacgtggcgc ctcgcggccc gctacgccga
    22861 cgagctgaac ctcgacgggc tgacgccggc ggaggtgaag gacgcgctgc cgacgatccg
    22921 cgctcggtgc gaggagatcg ggcgcgaccc ggcgtcgctc agcgtgtcgg tccatgtctg
    22981 gtgggagcag ctcgtcgcga gcggcgccgc ccgctccgac atgatcgcgg cgtaccgcga
    23041 tctcgggatc agccgcgtca tcgcgctcgt ccgcgacagc gctgacagtg atgaagcgct
    23101 ggaggggtgg gcacaggact gccggtcggc cggagcggag ctcgaatagg gccgctaacg
    23161 tgatcgcgat gcaccggctc atgagcgccc gcacgcggct gacgcttctc ggcggcgcca
    23221 tcggcggccc ggccctgctg ctggcctggc ccggtgtggc cctcgcccac cagctcaacg
    23281 agcgctatgc ggcgcccctg ccgctactcg cctacgtggg tggcgcggcc ctcgccgtgg
    23341 cgatgtcctt cgccttcatc atgctgcgca acgcgcccgc gccacgcgac gaggtgccag
    23401 tctcaaaccg cctcgtgccg cgctggctgc gcttggcgct gcaggggatc ggcctgatcg
    23461 gctggctgtg gatcgtgatc cagacgttcg gcggcgggtc gggcgacggc gatgtcgcgt
    23521 cgctcttcct gtgggtctac ggctgggtcg gcctggcgct catcagcgcg cttggcgggc
    23581 caatctgggc gtggctcgat ccgttctcga cgatccacca gatcctggga gcggtcgcgg
    23641 gcaggctggg catctcgggc ggggagagcg cgccctatcc ggcgcggctc ggcaagtggc
    23701 ctgcggtgat tgggctcgcg gtcgtcgtgt ggctggagct ggttgcacgc gtcgagggcg
    23761 gccgatcgct ggggctgttc ttgatcggct acacaatcat caccgtcgcc gcgatggcgt
    23821 acttcggccg acccgcatgg cgggccaacg gcgaaacgtt cagcgtgtgg tttggcgtgc
    23881 tcgggcggct ggcgccgttt gccctcgatg gcgagcccga ggagggccga gtcgtccgcc
    23941 ggccctatgc atcgggactg atgtccgcgc aatggacgat cgccgacgtg acgcttgtca
    24001 cccttgccac gggctcgatc atcttcgacg gcctcagcca gacgcagacg tacttcgacc
    24061 ttttcgtgaa gaacggcctg ttcgggtcga ccgagctccg cgatagcgtc acggccgtag
    24121 tcttcctggg cggcctcgtg gcgatagtgc tcgtgatggc acgccgtttg ggcgtggccg
    24181 cgctgggcgc cgggttgctg cctgtcgcgg tcggctacct ggtcgcgcac tatctcgcgt
    24241 acctcctggt cgacggccag cgcatcatcg ccgcgctcaa cgatccactg ctccgcggcg
    24301 ccaatctgct gccgctcgac ctggcgtttt gggagccgtc gcttttcctg ccaacggcgg
    24361 tgctgtggag catccagctc gccgccgtgg tcgggggcca catcgtcggc gcgtgggcag
    24421 gccatgcgca gatggcgcgc gccgagggtg gaaccacact cgtcgcgcag ctgccgctcg
    24481 cgatcctgat ggtggcgctg acgaccatca ccctgtggtc gctcggccag gcggtcctcg
    24541 taaccccaag gaactgagcg tccgcggccc acacgcggcc tagactcagc aagcaatgag
    24601 gggtgcgacg gagcagccac cgatcgagcg ccgattcacg ctgatgacgg gcaccgatcc
    24661 ggcgctcgct gcgggcgtgc tgtggtgggg gccgggcgac ccacagatgc agcttgggcc
    24721 gcacggcctg gtgcgcgcga tgcgcctcgc cagtgggccg gcgacgctcg agctcgcgtg
    24781 ggccgggcaa cgcgtcctgg ccaaggcctg gggcgacggc gcgagcgagg cgctggccct
    24841 ggcgccgggc ctcataggcg acctcgacga ggcgccgccg ttagctgcgc atcatcccat
    24901 cgtcgccgat ctggcgcgtc gcctgcccgg cctgcacctg acgcgcggtg cgccggtcat
    24961 cgagatgctc gtcaactcga tcatcggcca gaaggtcacg ggcctggaag cccggcgcag
    25021 ccagcggctg ctgctcgccc ggttgggtga gcctgcgccc ggaccgcacc ggctgcggct
    25081 gctgccgccg cccgagaggc ttgccggcat cccgtactgg gcgttccacc cgcttggtct
    25141 cgagcgacgc cgggcggaca cgatccgggc ggcggcagcc atcgcgcccc ggctcgaggc
    25201 gatccgcgcc atgtctgttg acgacgcccg ccgccggctc gagtcggtgc ccggaatcgg
    25261 gccgtggacg gcggcggaga ccatgcgcct cgcgttgggc gacccggatg cggtcagcgt
    25321 gggcgactac aacctgccgc gccaggtgtg ctgggtgctc gccagcgagc gcgacgggga
    25381 cgacgcgcgc atgctcgagc tgctggcgcc atacgccggc cagcgcgctc gagtggtgct
    25441 gctgatcgag cgctccggcc tgcgtcccgc acgccgggcg ccgcgttatg cgccccggcg
    25501 catcgcgggc atgtgaggta tcgaagttga aagggccgga tgggacaatc cgcgcgtgtc
    25561 caatcgcgtc tcgcgccgcc agggcgtcat ctacggtcca ccccggccgg agcctgaaga
    25621 gggcgccaat agcgcgctga tcggccgggt gttgggcatc ggcatggtgg cacttgccct
    25681 ggccgtgctc gccggcgcgt ttgtgctggt caaccgcccg cccgggccga cacccacgcg
    25741 ccaggtcgga cctaccgcca gtccgccacc aggttcgccc agcgcgctgc catc
//
LOCUS       DATBRX010000077         8423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2034126, whole genome shotgun sequence.
ACCESSION   DATBRX010000077 DATBRX010000000
VERSION     DATBRX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034126"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VIF08_02750"
     CDS             complement(<1..510)
                     /locus_tag="VIF08_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8134933.1"
                     /translation="MSGATLELRDLTLTYGIRRAVDSVSARVDAAEMVALLGPNGAGK
                     STLLRAVSGSGYGYGTGGARRAGQVLIDEVPLDEYDGASLARIVAVVPGQSRVAFPLR
                     VEELVALGRIPHEHPLLGPRAADRAAIDAAIERVGITLLRGRDVRELSLGERQLAVLA
                     MAVAQAPRLL"
     gene            complement(507..1589)
                     /locus_tag="VIF08_02755"
     CDS             complement(507..1589)
                     /locus_tag="VIF08_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEY8134934.1"
                     /translation="MSTSVVARRAAVARPAWIDWLRHRPAWLALAGLVALCLVMVAGV
                     MFGSVAVAPGDTLAILAHRLLGWPVVVTWPASAETIVVELRLPRVLMALAVGAALSLA
                     GVALQGLLRNPLADPYVLGTASGAALGAAIALLVPVEFALLGFGLIHVLAFAGALGAV
                     AIVYRVSRLGGLSSLSSVLLTGYAIGSLLAAGLAITMYLSGQNLRQIFFYLLGSFSLA
                     SWPQVVVGLPIIVLGSIVLLTRARSLNALLLGEETAAHLGLDVRRERLVLLAAASLVT
                     AASVAMTGLIGFVGLVVPHVVRLVSGPNARALLPLTAIWGAAFLAMADLISRVSGELP
                     VGVITGVIGAPFFLFVLRRYRGGYEL"
     gene            complement(1586..2599)
                     /locus_tag="VIF08_02760"
     CDS             complement(1586..2599)
                     /locus_tag="VIF08_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEY8134935.1"
                     /translation="MRRLTLRLLPLLTLLLVVAACARPAASPTFAPPPTPGPTAAATP
                     TAAPTAPGITLVDDEGTTIELPGIPETIVSLTPATTELLFGLGVGERLRGRTDLDLYP
                     AEATALPVVATYQGVRIEDVVAIGPDLVIAGGNNFTAAGDVTRLRDLGIPVLVVYAPD
                     LDGVLADVRLVGQAVGEEEAAEGVVGAIQTRIDEVTAAVAGLDRPRTFYEIGYEPEIY
                     GPAPDSFVADMVNLAGGEPITTTDPAVFAIPLERLVTLDPEVIVLGDAAYGTCPHSVA
                     TRAGWAGMTAVRDGQVRPADDIIITLPGPRLGEGLAALALAIHPDADIEPPTAGSEYC
                     SEL"
     regulatory      complement(2602..2748)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            2905..3852
                     /locus_tag="VIF08_02765"
     CDS             2905..3852
                     /locus_tag="VIF08_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134936.1"
                     /translation="MKPLIAAMLLALCVCGPVTAREPTGAQRTPDDTYIPPRTPAQAA
                     FERMKLGLVAQINAFSLTTTAQPLAGNTATTNMLCSTDCGDGSGGSGDGSEPDPDEPV
                     PGEGSTSGTTGFIVPDNRVVLAVRARHQSTAFWCGPASGQVVINYSRGYVYESLNGDS
                     TATNWRTQATIASWMKTNQTTGTLGGNLAAALNRPDAVLRPVPDWTYVYARNTDGEDM
                     HAKVVVDVDHFAMPLVIAVKPHKADAEYWLPSWPREASGAKHWIVIFGYDGLWDGTTD
                     PTLYYTESAGNGSKNPGVYTVSSMAMWKVNQYNASTIVW"
     gene            3852..5069
                     /locus_tag="VIF08_02770"
     CDS             3852..5069
                     /locus_tag="VIF08_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134937.1"
                     /translation="MRTTANGHFVLALMLVSAGCGSVAPSPSPSLTASSMASLQPSVP
                     PISTSTPSPTVPPTPPTSGEVTDLACDPGRLPEGFTGHVSAFASLADEIVWSGGPGRN
                     DNDLYRYVPGDSQPELLYLNPERTSSITSIVGGAAGYAFTDERWDGDEPRGWRLWLLA
                     GLGTDPLLIDQSSDDRQIAPTVAMNDAWIAWEVVHGSYENPVNELRVVSVADPLAPTT
                     LLSYPGRDVYMQFPNLWDDELWYGIADNDWEALTERPRVEVIDLSNPAAAPAVFGAGR
                     RAFMPAPGTNVVAWKSGGSDELAALNSGVLTLYWRATGDIDELPVPGPETAAARISYP
                     SVGNRFVAWWDDIQQRLYVYDLAERQFRRIAEYDWTSDERVVKQSLAGDLLVYMHYLS
                     DGERYLEWCVLPR"
     gene            complement(5089..5361)
                     /locus_tag="VIF08_02775"
     CDS             complement(5089..5361)
                     /locus_tag="VIF08_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134938.1"
                     /translation="MHSSVIGKIEKANRYARELDRITIDQMSLTFRGDNDSHRVTLDT
                     DRWQCTCHNFEALHSCAHVLAMQKILGQMLSEPAQTSFFAAEGVPA"
     gene            complement(5522..6619)
                     /locus_tag="VIF08_02780"
     CDS             complement(5522..6619)
                     /locus_tag="VIF08_02780"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEY8134939.1"
                     /translation="MSTETGRVLEDVAAIKRRDPGSMLDKINRFADQMDASWKLSRAL
                     ELPERHRAASAVAVLGMGGSAVCGDLVRAIFGDRLTVPIVSVRDYELPAWVGPTTLVV
                     AVSHSGATEETLAALGTALERRAPVAVITTGGPLGEVATRVNLPRLVFPNETPPRASL
                     GYTMVALAGVLERAGMLALTDDEVTAGVEAAREVARACAVDIDTSANLAKQLAWSLLD
                     RLPVIEGSGFLAAVARRWKTQLNENSNSTASAEELPEATHNTVVGYEQPDTLRDHHYI
                     VFLEGSGENPRNSRRARLSRDMLDAVGTSAQRVLFDQLTRFAQACAAIALGDFVSFYL
                     AMLYGVDPSATPALTVVKETLAEFDAVEGDD"
     gene            complement(6616..8097)
                     /locus_tag="VIF08_02785"
     CDS             complement(6616..8097)
                     /locus_tag="VIF08_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134940.1"
                     /translation="MSSAARPPIVFGTDGWRARIGEDYTYENVRRCAQGVAEWTRGVG
                     SSAKGVVIAYDRRFASEFFAQAAAEVLLAYDIPVAMAETAVPTQMTSYEVVERGAACG
                     IMITASHNPYPDNGFKVKSPSGAAASPDMLAVIEKTIGDNAGALPPTRSYAEALAGGK
                     VELYDPYPGYVEYTKRSLDLDRLKAADMRVLVEPMWGAGAGWISRLLAGGKIQVTEIH
                     QERNPFFGGVNPEPIAPNINESLGILKGGGYDLGLLLDGDADRAGAADEQGTFIHQLQ
                     VMGLLMYYMLEHRGLREPVVSTINETSMVGRLGKLYDVPVFETPVGFKYVGPKMIETG
                     AMMGGEESGGYGFGMHLPERDGIYADLLLLDLFVREREAGRWPVSAAVAHLHEIAGAS
                     FYLRTDVHVDKAVYPALKDRLLVELKAQAPSKIAGQGVVRTDALSTNDGFKFWTDDGS
                     WLLVRFSGTEPLVRVYAEAMSPQMRDEMLDQGARLVRGDGAEH"
     gene            complement(8094..>8423)
                     /locus_tag="VIF08_02790"
     CDS             complement(8094..>8423)
                     /locus_tag="VIF08_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134941.1"
                     /translation="AAEGQVVMGAMDVGWSDVGTWTALLDALVGGYTAPARVVPAGDA
                     AHLAADDLAVVRAEEDRLVLETGPGEVRSAQPMGLLPAARPHTAVVQDLLARVNDATS
                     APLKVNA"
BASE COUNT         1419 a         2942 c         2839 g         1223 t
ORIGIN      
        1 caagaggcgc ggcgcctgtg cgacggccat cgccagcacc gccagctggc gctccccaag
       61 ggacagctcg cgcacgtcgc gaccgcgcaa taaggtgatg cccacccgct cgattgccgc
      121 gtcgatcgcc gcgcggtcgg cggcgcgcgg gccgagcagc ggatgctcgt gcgggatgcg
      181 ccccagtgca accagctcct cgacacgcaa cgggaaggcg acgcgcgact gaccgggcac
      241 gacggcgacg atccgcgcga gggaggcgcc gtcgtattca tcgagcggca cctcgtcgat
      301 cagcacctgg ccggctcggc gtgcgccgcc ggtgccgtag ccgtagccac tgccgctgac
      361 cgcgcgtagc agcgtggact tgccggcgcc gttgggcccg agcagcgcga ccatctcagc
      421 cgcgtcgacg cgcgcgctga cgctgtccac cgcgcggcgg atgccatacg tcagcgtcag
      481 gtcacgcagc tcgagcgtcg cgccgctcac agctcgtacc cgccgcgata gcgccgcagg
      541 acgaacagga agaacggcgc gccgatgaca ccggtgatga cgcccacggg cagctcgccc
      601 gagacgcgcg agatgagatc ggccatggcg agaaaggccg cgccccagat cgcggttagc
      661 ggcagcaggg cacgcgcgtt cggcccggac accagccgca cgacatgggg caccacgaga
      721 cccacgaagc cgatcagccc cgtcatcgcc acggaggcgg ccgtgaccag cgaagcggcc
      781 gcgaggagca cgaggcgctc acgtcgcaca tcgagcccga ggtgcgcggc cgtctcctcg
      841 cccagcagca gcgcgttgag cgaacgcgcc cgcgtcaaca gcacgatcga gccgagcacg
      901 atgatcggca gtccgaccac gacctggggc cacgacgcga gcgagaacga gcccaggagg
      961 tagaagaaga tctggcgcaa attctggccg gacagataca tcgtgatcgc gagccccgcc
     1021 gcgagcagcg agccgatcgc gtagccggtc agcaggaccg agctgagcga cgagagaccg
     1081 cccaggcgcg acacgcggta gacgatcgcg acagcgccca gcgcgccggc gaacgccagg
     1141 acgtggatca gtccaaagcc gagcagcgcg aactcgaccg gcaccagcag cgcgatcgcg
     1201 gcgccaagcg ccgcgccgct ggccgtgccc agcacatatg gatcggcgag cgggttgcgc
     1261 agcaatccct gtagcgccac tcccgcgagc gacaacgcgg ccccaacggc cagggccatc
     1321 aggacccgcg gcagtcgcag ctcaacgacg atcgtctccg ccgacgctgg ccaggtcact
     1381 acgacaggcc agccgagcag acggtgagcg aggatcgcga gcgtgtcgcc gggcgcgacg
     1441 gcgacgctgc cgaacatcac cccggcgacc ataaccaggc acagcgccac gaggccagcc
     1501 agtgccagcc acgccggcct gtggcgcaac cagtcaatcc atgccggccg tgccaccgcg
     1561 gcgcgacgag cgacgacgga ggtactcaca gctcggagca gtactccgag cccgcagtcg
     1621 gtggctcgat gtcggcatcg ggatggatcg ccagcgccag cgccgcgagt ccctccccca
     1681 gccgcggtcc gggcagggtg atgatgatgt cgtcggctgg ccgcacctgg ccgtcgcgca
     1741 ccgcggtcat gcccgcccaa cccgcgcgcg tagcaacgct gtgggggcac gtcccgtagg
     1801 cggcgtcgcc gagcacgatc acctccgggt cgagcgtcac caggcgttcg agcgggatgg
     1861 cgaagacggc cggatcggtg gtcgtgatgg gctcgccccc ggccaggttg accatgtcgg
     1921 cgacgaacga atcaggtgcc ggcccataga tctccggctc gtagccaatc tcatagaaag
     1981 tccgcggcct atccaatccg gccacggcgg cggtgacctc gtcaatgcgc gtctggatgg
     2041 caccgaccac cccttcggca gcctcctcct cgcccaccgc ctggcccacg aggcgaacgt
     2101 cggccagaac gccatcgaga tcgggtgcgt acacgaccag gaccgggatg cccaggtcgc
     2161 gcagccgcgt gacatcgccc gccgcggtga agttgttgcc accggcgata accaggtccg
     2221 gcccaatcgc gacgacgtcc tcgatgcgca caccctggta tgtggcgacc acgggcagcg
     2281 ccgttgcctc cgccggatac aggtcgagat cggtccggcc acgcaagcgc tcgcccacgc
     2341 ccagcccgaa gagaagctcg gtcgtggccg gtgtgagcga gacgatggtc tcagggatgc
     2401 ccggcagctc gatggtcgtg ccctcgtcgt cgacgagggt gatcccgggc gcggtgggcg
     2461 cggcggtcgg agtcgcggcc gcggtcgggc cgggtgtggg cggtggcgcg aacgtcgggg
     2521 acgcggctgg tctcgcgcat gccgcgacga ccagcagcag tgtcaacagg gggagtaatc
     2581 ggagggtgag cctgcgcatg ggtgcgttcc ctttctcgcg aaggaacttg gcacgaccat
     2641 gggcccggcg accggactac cgacgctccg ttgagagcgc ggatcaccgt agcgggactg
     2701 tgccggattc acaccggctt cgcggatccc acggtcctgt ctttgattgg tcggcggagt
     2761 gtagacctcc gccggccgtt tcgtggacga gctggataag tcagcgataa gagggtcggc
     2821 cgatcatcgg cgccagcact gcccagcgac gcaggtgtcc cgcccggact tccgcccgga
     2881 acgcccgccg caggaggtag tgaattgaaa ccgttgatcg ccgccatgct cctcgcgctg
     2941 tgcgtctgcg gcccggtgac ggcgcgcgag ccgactggag cgcaacgcac gcccgatgac
     3001 acctacatcc cgccacggac gcccgcgcag gccgcattcg agcggatgaa gctcggtctt
     3061 gtggcccaga tcaacgcctt ctcgctcacc accaccgcac agccgctggc cggcaatacc
     3121 gccactacca acatgctgtg ctccacggac tgcggcgacg gcagcggcgg cagcggcgac
     3181 ggcagcgaac ccgatcccga cgagcccgtc ccgggcgaag gcagcaccag cgggacgacc
     3241 ggcttcatcg tgcccgacaa cagggtcgtg ctggcagttc gcgcgcgtca ccagtccact
     3301 gcgttctggt gcgggccggc cagcgggcag gtcgtcatca actacagccg cggctatgtg
     3361 tacgaaagtc tcaacggcga cagcaccgcc actaactggc ggacgcaggc cacgattgcc
     3421 agctggatga agacgaacca gacaacgggc acgctcggcg gcaacctggc cgctgcgctc
     3481 aaccgcccgg atgccgtgct gagacccgtt cccgactgga catacgtcta cgcgcgcaac
     3541 acggacggcg aggacatgca cgccaaggtc gtggtcgacg tggaccactt cgcaatgccc
     3601 ctggtgatcg cggtcaagcc ccacaaggca gacgccgagt actggctgcc ctcgtggcca
     3661 cgggaggcat cgggcgccaa gcactggatc gtgatcttcg gttacgacgg cctgtgggac
     3721 gggacgacag atccaaccct gtactacacc gagtcggccg gcaacgggag caagaacccg
     3781 ggcgtgtaca cggtgagcag catggccatg tggaaggtca accagtacaa cgccagcacg
     3841 atcgtctggt gatgcgcacg accgccaacg gccacttcgt cctggcgctg atgctggttt
     3901 cggctgggtg cggcagcgtc gcacccagcc ccagccccag cctcacggcg tcatccatgg
     3961 cgtcgctgca gccgagcgtc ccgcccatct cgacatcgac gcccagcccg acggtcccgc
     4021 ccaccccgcc aaccagcggc gaggtgacag atctggcatg cgacccgggg cgcctgcccg
     4081 aaggattcac gggccacgtt tctgcattcg cgagcctggc cgacgagatc gtgtggagcg
     4141 gtgggccagg ccgtaacgac aacgatctgt atcgatacgt ccccggcgac tcccaaccgg
     4201 agctgctgta tctcaacccc gagcgcactt catccatcac cagcatcgtc ggcggtgccg
     4261 ccggatacgc attcaccgac gagcgctggg acggcgacga gccgcgcggt tggcggctgt
     4321 ggctgctggc tgggctcggc actgacccgc tgctcatcga tcagagcagc gatgaccggc
     4381 agatcgcacc gacggttgcg atgaacgacg cctggattgc ctgggaggtg gtccacggca
     4441 gctacgagaa ccctgtcaac gagctgcgcg tggtgagcgt cgctgatccc ctggcgccca
     4501 cgacgctgct ctcgtaccca gggcgcgatg tctacatgca gttccccaac ctgtgggacg
     4561 acgagctctg gtacggcatc gctgacaacg actgggaggc ccttacggag aggccgcgcg
     4621 tggaggtgat cgacctgtcg aatccggcgg ccgcacccgc ggtgttcgga gccggccggc
     4681 gagcgttcat gcccgcgccg ggcacgaatg tcgtcgcctg gaagagcggc gggagtgacg
     4741 agctggcggc gctcaattcg ggcgtgttga cactctattg gcgagcaacg ggcgacatcg
     4801 atgagctgcc ggtgccgggc cccgaaacag ctgccgctcg gatcagctac ccgtccgtgg
     4861 gcaaccggtt cgtagcgtgg tgggacgaca tccagcagcg gctgtacgtc tatgacctgg
     4921 ctgagcggca gttccggcgc atcgccgagt acgactggac atcggacgag cgcgtggtca
     4981 agcagtcgct ggcaggggac ctgctcgtct acatgcacta cctgagcgac ggggagcgct
     5041 acctcgaatg gtgcgtcctg ccgcgttgag ccgcggctga gacgcgcctc aggcgggaac
     5101 accctccgcg gcgaagaagc tcgtctgggc gggttcgctc agcatctggc ccaggatctt
     5161 ctgcatcgcc aggacgtggg cgcacgagtg cagggcttcg aagttgtgac aggtgcactg
     5221 ccagcggtcg gtgtcgaggg tcacccgatg actgtcgttg tcgccccgga aagtgagcga
     5281 catctggtcg atcgtgatcc ggtccagctc gcgtgcatag cgattggcct tctcgatctt
     5341 gccgatcacg cttgagtgca tgagcaacct cccgcccggg actttgggcc gtcccgtacc
     5401 tgtttggcgc tcggtttcac ggcatccgct ccagccgtgc gagccgagcg attaggaggt
     5461 cagactaaca ccgccgaggg tcgacgcaac cagtccaaat ggccccgatt ggacctttcc
     5521 gtcagtcgtc cccctccacg gcgtcgaact cagcgagggt ctccttgacg accgtcagtg
     5581 cgggcgttgc cgaggggtca acgccgtaca gcatcgccag gtagaagctc acgaagtcac
     5641 ccagggcgat ggccgcgcac gcctgggcga agcgtgtcag ctggtcgaac aggacgcgct
     5701 gcgcagacgt gcccacggcg tcgagcatgt cgcgcgacag gcgggcgcgg cgcgagttgc
     5761 gcgggttttc gccgctcccc tcgagaaaga cgatgtagtg gtggtcgcgc aacgtgtcgg
     5821 gttgctcgta cccgacgacc gtgttgtgcg tcgcctccgg cagctcctcc gcgctcgcgg
     5881 tcgagttgga gttctcgttg agctgcgtct tccagcgccg cgcgacggcc gccaggaagc
     5941 cgctgccctc gatgaccggc agtcggtcga gcagtgacca ggcgagctgc ttggccaggt
     6001 tggcgctggt gtcgatgtcg accgcgcacg cgcgggccac ttcccgcgcg gcctcaactc
     6061 cggccgtgac ttcatcgtcg gtgagcgcaa gcatcccggc gcgctcgagc acgccggcca
     6121 gcgcgaccat cgtgtagccc agcgacgcgc gcggcggcgt ctcgttgggg aagaccaggc
     6181 gcggcaggtt gacgcgcgtg gcgacctcgc ccagcggacc accggtggtg atcaccgcga
     6241 ccggtgcgcg gcgctccagc gcggtgccga gggcggcgag cgtctcctcg gtcgcgcccg
     6301 aatgcgagac agcgacaacc agggtggtgg gacctaccca cgctggcagc tcgtagtcgc
     6361 gcaccgacac gattggcact gtcagccggt cgccgaagat ggcccgcacc aggtcgccgc
     6421 acacggctga gccgcccatg cccagcaccg cgaccgcgct cgccgcgcga tgccgctcgg
     6481 gcagctcgag cgcgcggctg agcttccagg acgcgtccat ctggtcggcg aagcggttga
     6541 tcttgtcgag catcgagccc ggatcgcggc gcttgatcgc cgccacgtcc tcgaggacgc
     6601 ggccggtttc ggtgctcaat gctccgcccc gtcgccgcgc accagtcgcg cgccctggtc
     6661 gagcatctcg tcacgcatct ggggcgacat cgcctccgcg tagacgcgca cgagtggctc
     6721 cgtcccgctg aagcgcacca ggagccacga tccgtcgtcg gtccagaact tgaagccgtc
     6781 gttcgtcgac agcgcgtcgg tccggactac gccctggccc gcgatcttgg acggcgcctg
     6841 cgccttcagc tcaacgagca atcggtcctt cagggcgggg tagacggcct tgtcgacgtg
     6901 gacgtcagtg cgcaggtaga aagacgcgcc tgcgatctcg tgcaggtggg ccaccgcggc
     6961 cgacaccggc cagcgtccgg cctcgcgctc gcgcacgaac aggtccagca gcagcaggtc
     7021 ggcatagatt ccgtcgcgct ccgggaggtg catgccgaag ccgtagccac ccgattcctc
     7081 gccgcccatc atcgccccgg tctcgatcat cttgggcccg acgtacttga agccgacagg
     7141 cgtctcgaag accggcacgt cgtacagctt gcccagccgt ccgaccatgg acgtttcgtt
     7201 gatcgtcgat acgaccggct cgcgcaggcc gcggtgctcg agcatgtagt acatcagcag
     7261 gcccatcacc tggagctggt ggatgaacgt gccctgctca tcggcagccc cggctcggtc
     7321 ggcatcgcca tcgagcagca atcccaggtc gtagccgcca cccttcagga tgcccagcga
     7381 ctcgttgatg ttgggcgcta tgggctccgg attcacaccg ccgaagaatg ggttgcgctc
     7441 ctggtggatc tccgtgacct ggatcttgcc gcccgcgagg aggcgcgaga tccagccggc
     7501 accggcaccc cacatcggct cgaccagcac gcgcatgtcg gccgccttta ggcggtccag
     7561 gtcgagcgaa cgcttcgtgt actcgacgta gcccggatat gggtcgtaca gctccacctt
     7621 cccgccggcc aacgcctccg catacgaacg cgtcggcggc agggcgccgg cgttgtcgcc
     7681 gatcgtcttc tcgatcacag cgagcatgtc cgggctcgcc gcagcgccac tgggcgactt
     7741 caccttgaag ccgttgtccg gatacgggtt gtgcgaggcg gtgatcatga ttccgcaggc
     7801 ggcgccgcgc tcgacaacct cgtacgacgt catctgcgtg ggcacggccg tctcagccat
     7861 cgccactggg atgtcgtagg cgagcagcac ctcggccgcg gcctgggcga agaactcgga
     7921 cgcgaaacgc cggtcgtagg cgatcaccac gcccttggcg ctgctgccca cgccccttgt
     7981 ccattcggcg acaccctggg cgcagcggcg cacgttctcg tacgtgtagt cctcgccgat
     8041 gcgggcgcgc cagccgtccg tgccgaagac gatgggtggc cgcgcggcgc tgctcacgcg
     8101 ttcaccttca acggcgccga cgtcgcgtca tttacgcgcg ccagcagatc ctgcacgacc
     8161 gcggtgtgcg gtcgcgcggc aggcagcagg cccatgggct gcgcggagcg cacctcgccg
     8221 gggccggtct cgaggaccag gcggtcctcc tcggcgcgca ccaccgcgag atcgtcggct
     8281 gccaggtgcg cggcatcacc ggctggcacg acacgcgcgg gcgcggtgta tccacctacg
     8341 agtgcgtcca gcagcgccgt ccacgtgccg acatcggacc agccgacatc cattgcgccc
     8401 atcaccacct ggccctccgc ggc
//
LOCUS       DATBRX010000078         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3040825, whole genome shotgun sequence.
ACCESSION   DATBRX010000078 DATBRX010000000
VERSION     DATBRX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040825"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VIF08_02795"
     CDS             <1..547
                     /locus_tag="VIF08_02795"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY8134942.1"
                     /translation="FGHGANWGLFGEDLPDEANTLVLSQTLTDSSGLAAAEIHYRMSE
                     NTRKMLDFHIERATESLLTAGAFKIETDRLMRYSGWHLLGTARMGEDRETSVVDRWNR
                     SHDVPNLYVVDGSVFVTSSGVNPTSTIVAIALRAAEHMVETRFEQQVPEQAKSMQAAS
                     WRDAGAERAAAAGGPAAESPS"
     gene            544..1038
                     /locus_tag="VIF08_02800"
     CDS             544..1038
                     /locus_tag="VIF08_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134943.1"
                     /translation="MREFDGDRLLTLARIADHLIPEAHGMPSAAEVVDAQRIAFVLNA
                     RPDLRVPLVMALRPELDADPAARLAALANDEPDSLAALQLVIVGGYYTDKHVRELIGY
                     NGQEAIEVKSWLMPQYLEEGLIDKVLERGPVWRDPSTGARAVVEDAPRTYAERFAATA
                     PDKE"
     gene            1043..2527
                     /locus_tag="VIF08_02805"
     CDS             1043..2527
                     /locus_tag="VIF08_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8134944.1"
                     /translation="MAVIASDQTAQQEHWLDSFLADWQTGTGEMHSVHEPATGRHLLD
                     LRLSTPDDVARAAAAAKAAQPAWAAASYQERSAVLRRAAEIYEAHRPEFGTWTMRETG
                     AHRNKMHHEQNFTAGEALAAAAMPWQPYGSLVPTVQQERLSMIRRVPVGVVGAITPWN
                     SPSVLGMRVVAPALALGNAVVLKPDPQTPVCGGAMFAAVFKEAGLPDGLLQIVIGDAE
                     AGQALVTDPNIDLVSFTGSTEVGRIVGAMAGGLLKKVSLELGGNNPLIILDDADLAAA
                     ASAGAYASFQFQGQVCFATGRHIVHRSVADEYVDLLAQKARRLRTGDPYRTDVELGPI
                     VNERQIKRVDDIVQRSIASGGARLVEGGTYEGLFYRPTVLADVHLDTPAWTDEIFGPV
                     APVVTFASDDEAIELANSSEYGLSAGVYSRSISRGLAIAQRIRAGMVHVNDQTINDEA
                     TIPFGGMGMSGNGGRFGAEANFDTFTQWQWLTVRDEPKSFGPTY"
BASE COUNT          452 a          923 c          880 g          386 t
ORIGIN      
        1 cttcggccat ggcgcgaact ggggcctctt cggcgaggac ctgcccgatg aggcgaacac
       61 cctggtcctg tcgcagacgc tgaccgactc atccggtctg gccgcggccg agatccatta
      121 ccggatgtcc gagaacacgc gcaagatgct cgacttccac atcgagcgcg cgaccgaatc
      181 gctgctgacc gccggcgcct tcaagatcga gaccgaccgg ctgatgcgct acagcggctg
      241 gcacctgctg ggcaccgcgc gcatgggcga ggaccgcgag acgagcgtcg tggatcggtg
      301 gaaccggtcg cacgatgtgc cgaacctgta tgtcgtcgac ggctcggttt tcgtcacctc
      361 gagcggggtc aaccccacgt cgacgatcgt cgcgatagcg ctgcgcgcgg cagagcacat
      421 ggtcgagacg cgcttcgaac agcaggtgcc ggagcaagcg aagagcatgc aggccgccag
      481 ttggcgggat gcaggcgcgg agcgcgcagc agcggccggc gggccagctg cggagtcacc
      541 cagttgaggg agttcgacgg ggaccgcctc ctgacgctgg ccaggatcgc cgatcacctc
      601 atccccgagg cacatggcat gccctcggcg gccgaagtcg tcgacgccca gcgaatcgcg
      661 ttcgtgctga acgcgcggcc cgacctgcgg gtgcccctgg tcatggcgct gcgaccggag
      721 ctggacgccg atcccgctgc gcgactggcg gcgctcgcca atgacgagcc ggatagcctg
      781 gccgcgctgc agctggtgat cgtcggcggc tactacaccg acaagcacgt gcgcgagctg
      841 atcggctaca acggccagga ggcaatcgag gtcaagtcgt ggctgatgcc gcagtacctc
      901 gaggaagggc tgatcgacaa ggtcctggag cgcggcccgg tgtggcgcga cccgtcgacc
      961 ggcgcgcgag cggtcgtcga ggacgcaccc cgaacctacg ccgagcgttt cgccgcaacc
     1021 gcgcccgaca aggagtaacg aaatggccgt gatcgcgtcc gatcagaccg cgcagcaaga
     1081 gcactggctc gactccttcc tcgccgactg gcagaccggc acgggcgaga tgcacagcgt
     1141 ccacgagccc gcgaccggca ggcacctgct tgacctgcgc ctgtcgacgc ctgacgacgt
     1201 cgcccgtgcc gcggccgcgg cgaaagccgc gcaaccggcg tgggccgcgg cctcctacca
     1261 ggaacgctcg gcagttctgc gccgcgcagc tgagatctac gaagcgcacc ggcccgagtt
     1321 cggcacctgg acgatgcgcg agaccggcgc ccaccgcaac aagatgcacc acgagcagaa
     1381 cttcaccgcc ggtgaggcgc tggccgcagc ggccatgcca tggcagccct acggctcgct
     1441 cgtgccgacc gtccagcagg agcgcctgag catgatccgc cgcgtgccgg tgggcgtcgt
     1501 cggcgccatc acgccgtgga actcgcccag cgtcctgggc atgcgcgtcg tcgccccggc
     1561 gctcgccctg ggcaacgccg tcgtcctcaa gccggatcca caaaccccgg tctgcggcgg
     1621 cgcgatgttc gcggccgttt tcaaggaggc cggcctgccc gacgggctgc tccagatcgt
     1681 catcggcgac gctgaggccg gtcaggccct cgtcaccgat cccaacatcg atctggtcag
     1741 cttcaccggg tcgacggaag tcgggcggat agtcggtgcc atggccggtg gcctgctcaa
     1801 gaaagtcagc ctggagctcg gcggcaacaa cccgctgatc atccttgacg acgccgatct
     1861 cgccgcggcc gccagtgcgg gcgcgtacgc gagcttccag ttccagggcc aggtgtgctt
     1921 cgcgaccggg cgccacatcg tccatcgcag cgttgccgat gaatacgtgg atctgctcgc
     1981 ccagaaggcc agaaggctgc gcactggtga cccgtaccgg acggacgtgg agcttggccc
     2041 catcgtcaac gagcgccaga tcaagcgcgt cgacgacatc gttcagcgct ccatcgcgag
     2101 tggcggggct aggctcgtgg agggcgggac ctacgagggc ctcttctacc gtcccaccgt
     2161 ccttgccgac gtccacctcg acacaccggc ctggaccgac gagatcttcg gccctgtcgc
     2221 gccggtcgtg accttcgctt ccgacgatga ggcgatcgag ctcgccaact catccgagta
     2281 cggactgtca gcgggcgtct actcgcgctc gatctcgcgc ggcctcgcaa tcgcgcaacg
     2341 gattcgcgcg ggcatggtcc acgtcaacga ccagacgatc aacgacgagg ccacgatccc
     2401 gttcggcggc atgggcatga gtggcaacgg tggccgtttt ggcgcggagg ccaacttcga
     2461 caccttcacc cagtggcagt ggctgaccgt gcgcgacgag cccaagtctt tcggccccac
     2521 gtactaggca ccgaccacca gccatggctt cgatcaccga ggttgccaag ctcgccgggg
     2581 tctccacggc gacagcgtcg cgcgtcgtcg gcgaggtcga ctacccggtc agcgaaacga
     2641 c
//
LOCUS       DATBRX010000079        19875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1923981, whole genome shotgun sequence.
ACCESSION   DATBRX010000079 DATBRX010000000
VERSION     DATBRX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923981"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /gene="tuf"
                     /locus_tag="VIF08_02810"
     CDS             <1..142
                     /gene="tuf"
                     /locus_tag="VIF08_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY8134945.1"
                     /translation="EMVMPGDNVDMGVELITPIALETGQRFAIREGGHTVGAGAIIEI
                     EA"
     gene            147..455
                     /gene="rpsJ"
                     /locus_tag="VIF08_02815"
     CDS             147..455
                     /gene="rpsJ"
                     /locus_tag="VIF08_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY8134946.1"
                     /translation="MARQRIRIRLKAYDHRLLDQSALQIVETAQRTGADVVGPVPLPT
                     RIEKFTVIRSPFIDKDSREQFEIRTHKRLIDILDPSAKTVDALMRLSLAAGVDIEIKM
                     "
     gene            464..1096
                     /gene="rplC"
                     /locus_tag="VIF08_02820"
     CDS             464..1096
                     /gene="rplC"
                     /locus_tag="VIF08_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY8134947.1"
                     /translation="MSIGVIGKKVGMTSVFAEDGTMVPVSVVAVEPNTVTGLRTAERD
                     GYVAVQLGAHKPRRLTKPRAGQLKGLTPVKDVREFRLDDASDYQVGQTLDVSLFAEGE
                     TVDVTGVSKGRGFSGTIKRHNFRRGPETHGSDSHRQPGSVGAGTYPGKVFKGSGMAGR
                     MGNDTVTVKKATIVKADAERNVLLVKGPLPGARNALIVIRKITKAGKKAK"
     gene            1096..1719
                     /gene="rplD"
                     /locus_tag="VIF08_02825"
     CDS             1096..1719
                     /gene="rplD"
                     /locus_tag="VIF08_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY8134948.1"
                     /translation="MPSTTLYNKQGKSVGNVDLPETLFAADINQTVMHQAVTAQMNAR
                     RAGTSDTKTRGEVRGGGAKPYRQKGTGRARQGSTNAPHYSGGGVVFGPHPRSYEQRLP
                     KRMKRMALHGALTSKFGDGAVKVVDELGLDEPKTQVLVGYLDALAATGRVLVVANGSD
                     ANLDLSARNIPGVSIIRPDSLNIVDVMNADVLLITQPSLATMAEVYA"
     gene            1716..2021
                     /gene="rplW"
                     /locus_tag="VIF08_02830"
     CDS             1716..2021
                     /gene="rplW"
                     /locus_tag="VIF08_02830"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY8134949.1"
                     /translation="MTLQSAEIILRPVISEKSMDETQRGKYTFRVHSAANKHQVKAAI
                     EDLFKVDVVTINVSTTQPKEKSRNRRGGGRVEGWTSKWKKAVVTLAAGQKIEFFEGV"
     gene            2024..2854
                     /gene="rplB"
                     /locus_tag="VIF08_02835"
     CDS             2024..2854
                     /gene="rplB"
                     /locus_tag="VIF08_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY8134950.1"
                     /translation="MPLRSYKATSAGRRFQTRPTFEEITTDKPHKPLLEPQQRISGRN
                     SQGRMTVRHRGGGEKRHYRRIDFKRDKLDVPAVLATIEYDPNRSARIGLLHYRDGDKR
                     YMLVPNGLNVGDTVISGNKAEARVGNALPLKAIPLGTTIHNIEMVPGRGAQMVRSAGT
                     AAQLLAKEGDHAQVRMPSGEVRRVDVRCMATIGQVGNLDHENQSLGKAGRSRHIGRRP
                     SVRGVAMTPRDHPHGGGEGKSPTGMPPKTPWGKPAMGHRTRTNKLTGRNIVRSRHRKS
                     "
     gene            2871..3155
                     /gene="rpsS"
                     /locus_tag="VIF08_02840"
     CDS             2871..3155
                     /gene="rpsS"
                     /locus_tag="VIF08_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY8134951.1"
                     /translation="MSRSLKKGPYVEARLLSNVQQMNKKNEKKVLKTWSRASVIFPDF
                     VGHTLAVYNGKKHIPVYITENMVGHRLGEFSPTRSFRGHGKSTERSSALK"
     gene            3164..3502
                     /gene="rplV"
                     /locus_tag="VIF08_02845"
     CDS             3164..3502
                     /gene="rplV"
                     /locus_tag="VIF08_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY8134952.1"
                     /translation="MKVKATAKFQRLSTRKTRLVTQSIIGLPVAEASALLKHMTQGPA
                     RTVAKVLSSAAANAENNHDLAPDDLYVAEATANEGPTLKRFHPRAQGRAFAIHKPMTH
                     VTVVVENREA"
     gene            3506..4174
                     /gene="rpsC"
                     /locus_tag="VIF08_02850"
     CDS             3506..4174
                     /gene="rpsC"
                     /locus_tag="VIF08_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY8134953.1"
                     /translation="MGRKVHPYGFRLGTSRTWNAKWYADKNYTTLLKEDITIRQLVAK
                     RLANASVSLVDIERGINHVTVTVHTAKPGIVIGKGGANVEALRQQIGRITTSKVKLEI
                     KEIRQPELDAVLVAANIAQQLNRRIAFRKALKQSIQRTMKAGAKGVRIAVSGRLGGSE
                     MARREWDKEGRIPLNTLRADISYGQVHAHTTYGRIGVKVWIYRGDIMPTRQAPPEAAA
                     QGAA"
     gene            4177..4602
                     /gene="rplP"
                     /locus_tag="VIF08_02855"
     CDS             4177..4602
                     /gene="rplP"
                     /locus_tag="VIF08_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY8134954.1"
                     /translation="MLMPKRVKHRRVQRGRMSGLAKGGYTIAFGDYALMTEEPAWITA
                     RQIEAARRAMTRYVKRGGKIWIRVFPDKPVTKKPAEVRMGSGKGAPDHWVAVVKPGRI
                     LFEMSGVPVKEASEAMRLAAMKLPVNMKFLMREGVGDGH"
     gene            4592..5005
                     /gene="rpmC"
                     /locus_tag="VIF08_02860"
     CDS             4592..5005
                     /gene="rpmC"
                     /locus_tag="VIF08_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY8134955.1"
                     /translation="MDIDSVRNLTDTELLGELVTQRRALYDLRFQLATRQLTDHSQLT
                     QTRRSIARVLTVMTERSLDETMVPRTASPKPAARAAAAKAAAKSAAKPAAKSAGKAST
                     TSKDSAAKGAAATEAATKSTTGAETTADAAKGDEE"
     gene            5002..5256
                     /gene="rpsQ"
                     /locus_tag="VIF08_02865"
                     /pseudo
     CDS             5002..5256
                     /gene="rpsQ"
                     /locus_tag="VIF08_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            5397..5765
                     /gene="rplN"
                     /locus_tag="VIF08_02870"
     CDS             5397..5765
                     /gene="rplN"
                     /locus_tag="VIF08_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY8134956.1"
                     /translation="MIQQYSRLKVADNTGARVIQCIRVLGRSQKTTAGVGDVIVASVK
                     QAIPNAAVKKGDVVQAVIVRTTKELGRPDGSYIRFDENAAVLINNQNNPRGTRIFGPV
                     ARELRDRGYMKIVSLAPEVL"
     gene            5765..6238
                     /gene="rplX"
                     /locus_tag="VIF08_02875"
     CDS             5765..6238
                     /gene="rplX"
                     /locus_tag="VIF08_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY8134957.1"
                     /translation="MARTQPPARDMRVPDIRKGDQVLVLTGREAGKKGTVEHVVRNGQ
                     GFKKSVAKYGSGWRKVSPLASVAVVVEGLNIAKVHTKPRQKQGRTERQPRIQQGGILD
                     VAQPILASKVMIICPQCSQPTRIKHGVAGDGRSIRLCTNCGETLTTETRKETRKK"
     gene            6547..7086
                     /gene="rplE"
                     /locus_tag="VIF08_02880"
     CDS             6547..7086
                     /gene="rplE"
                     /locus_tag="VIF08_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017434009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY8134958.1"
                     /translation="MHGLHLRYRDEVAPALQKEFSYSNPMQVPRLDKIVVNIGLGEAL
                     TNAKALDAAVGDLAAITGQKPIVTRARRSIAQFRVRTGNPIGAKVTLRGQRMWDFLER
                     LTRLALPRIRDFRGVPDKSFDGRGNYSLGLREQLAFPEIDYDKIDKLRGLEVSIVTTA
                     RTDEESKRLLQLLGMPFAN"
     gene            7143..7328
                     /locus_tag="VIF08_02885"
     CDS             7143..7328
                     /locus_tag="VIF08_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY8134959.1"
                     /translation="MAKKSLIAKAKRKPHQTSQGYNRCFVCGRPRGYMRRFGLCRICF
                     RERALLGELPGVTKSSW"
     gene            7339..7728
                     /gene="rpsH"
                     /locus_tag="VIF08_02890"
     CDS             7339..7728
                     /gene="rpsH"
                     /locus_tag="VIF08_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010169919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY8134960.1"
                     /translation="MSDPIADMLTRIRNGSRARHSEILVPASRTKRAIAQILSDEGFI
                     DGFEDAQDGNHQQLKLHLKYVGKVPVISGMKRVSKPGLRVYATKTDIPRVLGGLGVVI
                     VSTSHGIMTGARARQAQLGGEVLAYVW"
     gene            7758..8309
                     /gene="rplF"
                     /locus_tag="VIF08_02895"
     CDS             7758..8309
                     /gene="rplF"
                     /locus_tag="VIF08_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY8134961.1"
                     /translation="MSRIGRLAIPIPAGVTVDVEGSHVAVKGPKGMLERDLSPELRIV
                     KDDGTLRVERPNDEKKSRELHGLTRTLLNNMVIGVTEGFRKPLEITGVGYRAQLVGKK
                     LQLNLGYSHPIEIDPPAGISFDVESPIKLAVVGIDKELVGHIAARIRSTRKPEPYKGK
                     GVRYAGEVIRRKAGKAGKIGGKK"
     gene            8306..8668
                     /gene="rplR"
                     /locus_tag="VIF08_02900"
     CDS             8306..8668
                     /gene="rplR"
                     /locus_tag="VIF08_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY8134962.1"
                     /translation="MSLATSRGTSRHKRHARLRLTLSGSPERPRLAVFRSINQIYAQL
                     IDDTAGKTLASASSLEGGLRDRKGTKTEDAKQVGKLLAERARAAGVTKVVFDRAGFRY
                     HGRVRALADAAREAGLDF"
     gene            8688..9236
                     /gene="rpsE"
                     /locus_tag="VIF08_02905"
     CDS             8688..9236
                     /gene="rpsE"
                     /locus_tag="VIF08_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY8134963.1"
                     /translation="MPRIDPARLTLEERVVQINRVAKVVKGGRRFSFSAVVVVGDGAG
                     HVGVGLGKAGEVPEAIRKGVEDAKKHLIKVPLVGTTIPHAVTQQYGASTVMLKPASQG
                     TGVIAGGSVRAVVIAAGIRDILSKSLGSTNPVNVVRATHEALRGMSSAEEIGARRGKK
                     LRSVISGQPGAGPRAEATANAG"
     gene            9226..9471
                     /gene="rpmD"
                     /locus_tag="VIF08_02910"
     CDS             9226..9471
                     /gene="rpmD"
                     /locus_tag="VIF08_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY8134964.1"
                     /translation="MPAKLKVTLIKSPISHTQRTRATMSALGLHRIGQSVVIDDTPEM
                     RGMTRAIRFLLRTEPTDGAETAAKAKSAARKKEASKS"
     gene            9468..10124
                     /gene="rplO"
                     /locus_tag="VIF08_02915"
     CDS             9468..10124
                     /gene="rplO"
                     /locus_tag="VIF08_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY8134965.1"
                     /translation="MKLHDLRPAAGARKARTRVGRGIAAGQGKTAGRGTKGQRSRAGA
                     SIPAWFEGGQTPIHIRVPKLHGFKNKFRVQYAVVNVGQISAYAEAGRLGDAKPPTVNA
                     ETLAQAGLIRGDGRPLKVLGQGEVATKLFVAADAFSASAKTKIEAAGGNVLVLAEPKP
                     EKVAKPDKADKSAKKAKPAQADAGEEANGSESPKAESEPQPEAAAEVSDSSESTESDN
                     "
     gene            10149..11444
                     /gene="secY"
                     /locus_tag="VIF08_02920"
     CDS             10149..11444
                     /gene="secY"
                     /locus_tag="VIF08_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY8134966.1"
                     /translation="MIEALINAFRAPDIRRRILFVLVMLVVYRLLAVVPVPNVNHQAL
                     DQLINNQTFLQLLDLFSGGGLRSFSIVAMGVNPYINASIIMQLMQGVVPRLQALSREG
                     EYGRTRLNQYTRYLTVPLALLQAYGYLAILSQSQIVEPIDILSPTTLTQMITLAGGTL
                     LAMWIGELITERGIGNGISFLIFAGIVARIPQGVAAFLQAPDIPAGILFVILALVVVA
                     VIVYIQEGQRRIPIQYASRVRGRRMYQGGATFLPLRVNQAGVIPIIFAVSILLFPTQI
                     ASYFATPPNAEPSLVNSIADGIVGFLSPNGIPYMVFYFLLTMGFTYFYTAFTFKPDET
                     ADQLRKNGGFIPGIRPGAPTRDYLQKVVFRLSLAGAIFLALIAVSPIVLSSLFAGQGV
                     VGGFALGGTALLIVVSVVVETMKQIEAQLMMRNYEGFIR"
     gene            11447..12109
                     /locus_tag="VIF08_02925"
     CDS             11447..12109
                     /locus_tag="VIF08_02925"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY8134967.1"
                     /translation="MYVMVGAPGAGKGTQAALLSERLGMPHVASGDLFRDNIKRGTAL
                     GKKVKGYLESGALVPDSVTVQMISDRLAKPDAADGAILDGFPRTRPQAEALDAMLAKM
                     DGEVGAALYVDVARDELLRRLSGRWICTVSPDHVYHSVARPPKVEGVCDIEGAPLYQR
                     DDDKPATVQTRLEQQLPPMYEVVDYYTDRGVLSTVDGAGEVAQVTDALLHAIAQPARL
                     QG"
     gene            12114..12941
                     /gene="map"
                     /locus_tag="VIF08_02930"
     CDS             12114..12941
                     /gene="map"
                     /locus_tag="VIF08_02930"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8134968.1"
                     /translation="MTPDRRVTLKSSAQIERMAIAGALVASVLDRLAAEVKPGVTTLQ
                     LDAIAEEMIRAAHGVPSFVGVPGRVAPYRHTLCVSLDDEIVHGVPGPRRIREGQIVSV
                     DAGAVVDGWHGDAARTFIVGEVPDHTRRLVETTERAMYAGIAAAQPGAFLADISGAIE
                     DIGRDAAYGVIRAFVGHGIGTEMHEEPQVTNYRTGSRGRRIEPGLCLAIEPMFTLGGH
                     EVRIREDGWTVVTADGSLAAHWENTIAVTADGPRVLTASAPAFMPVASGAPEALPTR"
     gene            13077..13310
                     /gene="infA"
                     /locus_tag="VIF08_02935"
     CDS             13077..13310
                     /gene="infA"
                     /locus_tag="VIF08_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY8134969.1"
                     /translation="MAKKDAIEVEGTVIEPLPNAMFAIEIDAADKVNKHRVLAHISGK
                     LRMNFIRILPGDRVRVELSPYDLSRGRITYRLK"
     gene            13321..13434
                     /gene="rpmJ"
                     /locus_tag="VIF08_02940"
     CDS             13321..13434
                     /gene="rpmJ"
                     /locus_tag="VIF08_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEY8134970.1"
                     /translation="MKVSASVKTRCDNCKIIRRHGVVMVICTNPKHKQRQG"
     gene            13444..13815
                     /gene="rpsM"
                     /locus_tag="VIF08_02945"
     CDS             13444..13815
                     /gene="rpsM"
                     /locus_tag="VIF08_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY8134971.1"
                     /translation="MARISGIDIPREKRVEVALTYIYGIGLPTSQKILAQANVNGDTR
                     VRDLTEDQVNRLREIIDRRYKVEGDLRREVALNIKRLQEIGSYRGLRHRRNLPARGQR
                     TKTNARQRRGPRRTVGVKRKK"
     gene            13833..14228
                     /gene="rpsK"
                     /locus_tag="VIF08_02950"
     CDS             13833..14228
                     /gene="rpsK"
                     /locus_tag="VIF08_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017795137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY8134972.1"
                     /translation="MAEKKGAKKTRRREKKNVPAGHAHIQASFNNTIITITDLAGGTV
                     SWGSAGVAGFKGSRKSTPYAAALSAEGAARKAMEHGVRQVEVFVKGPGAGREQAIRSL
                     QAAGIEVTAITDVTPIPHNGNRPPKRRRV"
     gene            14244..14870
                     /gene="rpsD"
                     /locus_tag="VIF08_02955"
     CDS             14244..14870
                     /gene="rpsD"
                     /locus_tag="VIF08_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY8134973.1"
                     /translation="MSRYRGPVSRLCRREGMKLFLKGAKCYTKKCPFERRPNPPGQHG
                     VRRRKVSDFGLQLREKQKMRRIYSVHERQFRNYFNEAERHTGVTGEALLRMLETRLDN
                     VVFRMGFSTSRAQARQLVSHGHFMVNGTATNIPSYAVAEGDRVEVREASRKSEYFKNA
                     RETLKGAQRPDWLSVDPDKLSGSVNAQPRRDQMPAELNEQLVVEFYSR"
     gene            14908..15903
                     /locus_tag="VIF08_02960"
     CDS             14908..15903
                     /locus_tag="VIF08_02960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY8134974.1"
                     /translation="MIELENPQIEAVGETQDWYAKYEASPLPAGYGVTIGNALRRVLL
                     SSLEGAAVTSIQVRDVYHEFSTIPGVVEDVTAIVLNVKKLRLKSYAPHPVQLRLSKSG
                     AGAVTARDIEANADVEIINPDQHLLTLDSDDAPVEIDMTVERGVGYLAAERSEQLPIG
                     VIPVDAIFSPVRKVNYTVENTRVGQMTNYDKLTIEIETDGTLTPEEALSRAAEILVGQ
                     FSLFTAAGKQMAATESGLPGAPQLPPNMLDMPIEELDLPMRAYNSLKRNNIVKVGQLL
                     QLQDEDLLRMRNFGKKSLDEMKERLRMRGFLPPDEGGDAAEFDPESGLDDLDEEE"
     gene            15908..16261
                     /gene="rplQ"
                     /locus_tag="VIF08_02965"
     CDS             15908..16261
                     /gene="rplQ"
                     /locus_tag="VIF08_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010285110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEY8134975.1"
                     /translation="MAHCIAGRKLGRKTGPRKALFKNLIVAVLRYEQIKTTEARAKEI
                     RGQVEHVITLAKDGSLAARRQIIAQLPDEPLVIDKLINEIAPKYSDRTSGYTRIIKIG
                     PRNGDAAPMVQLELV"
     gene            16281..17120
                     /gene="truA"
                     /locus_tag="VIF08_02970"
     CDS             16281..17120
                     /gene="truA"
                     /locus_tag="VIF08_02970"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8134976.1"
                     /translation="MGGTGKSGRKAAPRKCAREGVPVRYRARVEYDGTDFAGFQVQPG
                     GRTVQGELESALRRLAGGTRVVVDGSGRTDAGVHARGQVIAFTYTGRLGRSELQKALA
                     AGLPRDIGIGPIIPVGQDFSPRHQARHREYRYLIWNGRRSPLRERQSLGVRERLDVAA
                     MAAAAKVFIGRHDFSAFGGADRQPVRTLTKLSVVRRGDLITVTVIGDAFLRGMVRRIV
                     AALLRVGHGRASAEDLAQALGERRPAFNGEAAAPRGLTLWRVPIGPRRHKTRKREEQD
                     NQE"
     gene            17120..17566
                     /gene="rplM"
                     /locus_tag="VIF08_02975"
     CDS             17120..17566
                     /gene="rplM"
                     /locus_tag="VIF08_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY8134977.1"
                     /translation="MSRTYSPRASEIDRHWFVVDATNQRLGLLASRIAFVLEGKHKPT
                     YATHIDTGDHVIVLNAAKIDVGGKKSEQKTYSRHSGYPGGYREETLADLLTRRPEEVI
                     RRAVKGMLPRNRLGVQQLRKLKIYAGTEHPHEAQQPEPLPMGGSTR"
     gene            17566..17970
                     /gene="rpsI"
                     /locus_tag="VIF08_02980"
     CDS             17566..17970
                     /gene="rpsI"
                     /locus_tag="VIF08_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY8134978.1"
                     /translation="MAAGTTSFFYGTGRRKTSIARVRLLAGEGEIVVNGRSLEEHFGN
                     AIDLTEVTMPFKVTNTEGRYNAMIKVEGGGHHGQAGAIRHGIARALLQSDPEGVRAPL
                     RSAGLLTRDPRMKERKKFGLKRARKAPQYTKR"
     gene            18414..19091
                     /locus_tag="VIF08_02985"
     CDS             18414..19091
                     /locus_tag="VIF08_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8134979.1"
                     /translation="MVDVYGKYAETLRAGHQFAASGQLKEALRCYRAATELAGERALP
                     HVALGGTLLRLNQPREALAAYERALELEPDQIDALTGRAAALLAAGRRTEAAQVQERI
                     RQGPGSGPNSAAGLATPGSSADRYLASGEQARAAGKTDAAIDAWLAESAEHRVAGHHD
                     AALDACLRALALDSSAPRTHLEIARAWLARGWPDRAAQRLQLLERLLVLQPDKEVAAA
                     ARELFAG"
     gene            19171..>19875
                     /gene="cdaA"
                     /locus_tag="VIF08_02990"
     CDS             19171..>19875
                     /gene="cdaA"
                     /locus_tag="VIF08_02990"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEY8134980.1"
                     /translation="MDALLELVKSLQVEPIAFLDIGLTALLIYGLFSLIRGTRAVRLV
                     IGVTVLYVIYVLAQAFGLQLLSQILQAGAVVGLLALVVVFQPELRRALERIGRFGSMG
                     WLLAPGARASSERVGAVVAKAAVALGARKNGALIVIERETGLTDAAETGIMLDAQLTV
                     ELLETIFTPHSALHDGAAIVRHERVVSAGVVLPLSESGVYRERFGTRHRAALGITEQT
                     DAIAVVVSEETGSVSLV"
BASE COUNT         3624 a         6896 c         6599 g         2756 t
ORIGIN      
        1 ggagatggtc atgcccggcg acaacgtcga catgggggtc gagctcatca ccccgatcgc
       61 gctcgagacc ggccagcgct tcgccatccg cgagggcggc cacaccgtcg gcgccggcgc
      121 gatcatcgag atcgaggcct aacaagatgg cacggcagcg catccgcatt cgcctcaagg
      181 cctacgacca ccggttgctg gaccagtcgg cgcttcagat cgtcgagacg gcccagcgca
      241 ccggcgcgga cgtggtcggc ccggtgccgc tgcccacgcg catcgagaag ttcacggtga
      301 tccgctcacc gttcatcgac aaggacagcc gcgagcagtt cgagatccgg actcacaagc
      361 ggctgatcga catcctcgac ccgtcggcga agaccgtcga cgccctgatg cgactcagct
      421 tggccgctgg tgtcgacatc gaaatcaaga tgtagcgagt ccaatgagca ttggtgtaat
      481 cggtaagaaa gtcggcatga cctcggtctt cgccgaggac ggcaccatgg tgccggtctc
      541 ggtcgtggcc gtggagccga atacggtcac cggtctgcgc actgccgagc gcgacggcta
      601 tgtcgccgtg cagctcggcg cgcacaagcc gcgtcgcctg accaagccgc gtgccggcca
      661 gctcaagggc ctgacgccgg tcaaggacgt acgcgagttc cggctcgacg acgcgtccga
      721 ctaccaggtc ggccagacgc tcgacgtgtc gctgttcgcg gagggtgaga cggtcgacgt
      781 gaccggcgtt tccaaggggc gcggcttctc cggcaccatc aaacgccaca actttcggcg
      841 cggtccggag acgcacggct ccgactcgca ccgccagccg ggctcggttg gtgcgggcac
      901 gtacccgggc aaggtgttca agggatcggg catggcgggg cggatgggca acgacaccgt
      961 caccgtcaag aaggcgacca tcgtcaaggc cgacgccgag cgcaacgtgc tcctcgtgaa
     1021 ggggccgctg cccggcgcgc gcaacgcgct catcgtgatc cgcaagatca ccaaggccgg
     1081 gaagaaggcc aagtgatgcc cagcacgacg ctctacaaca agcagggcaa gtccgtcggc
     1141 aacgtcgacc tgcctgagac cctcttcgcg gccgacatca accagacggt gatgcaccag
     1201 gccgtcacgg cgcagatgaa cgcgcgccgg gccggcacgt cagacaccaa gacgcgcggc
     1261 gaggtccgcg gcggcggcgc caagccgtat cgccagaagg gcacgggccg cgcccgccag
     1321 ggctcgacca acgcgcccca ttactcgggc ggcggcgtgg tcttcggtcc gcatccgcgc
     1381 tcgtacgaac agcgcctgcc caagcgcatg aagcgcatgg cgctccatgg cgcgctgacc
     1441 tcaaagttcg gcgacggcgc ggtcaaggtc gtcgacgagc tcggactcga cgagcccaag
     1501 acccaggtcc tcgtcggcta cctcgacgcg ctcgcggcga ccggccgcgt gctggtggtc
     1561 gcgaatggca gtgacgccaa cctcgatctg tcggcgcgca acatcccggg cgtgtcgatc
     1621 atccggcccg actcgctcaa catcgtcgac gtcatgaacg ccgatgtgct gctcatcacc
     1681 cagccgtcgc tggcgacgat ggcggaggtg tacgcatgac gctccagtcg gccgagatca
     1741 tcctgcgccc ggtcatcagc gagaagtcga tggatgagac gcagcgcggc aaatacacat
     1801 tccgcgtcca cagtgcggcc aacaagcacc aggtcaaggc cgcgatcgag gacctcttca
     1861 aggtcgacgt cgtgacgatc aacgtctcca cgacgcagcc caaggagaag tcgcgcaacc
     1921 gccgtggcgg tgggcgtgtc gagggctgga cctcgaagtg gaagaaggcc gttgttaccc
     1981 tggccgcggg ccagaagatc gaattcttcg agggggtgta gggatgccgc tgcgcagcta
     2041 caaggcaacc tccgccggcc gccgtttcca gactcggccg accttcgagg agatcaccac
     2101 cgacaagccg cacaagccgc tgctcgagcc gcagcagcgc atcagcggcc gcaacagcca
     2161 gggccgcatg accgtgcgcc atcgcggtgg cggcgagaag cgtcattacc ggcgcattga
     2221 cttcaagcgc gacaagctcg acgtgcccgc agttctggcg acgatcgaat acgaccccaa
     2281 ccgctcggcg cgcatcggcc tgctgcacta ccgcgacggc gacaagcgct acatgctcgt
     2341 gcccaacggc ctgaacgtgg gcgataccgt catttccggc aacaaggccg aggcgcgcgt
     2401 cggcaacgcg ctgccgctca aggccatccc gctcggcacg acgatccaca acatcgagat
     2461 ggtgccgggc cgcggcgccc agatggtccg ttctgccggc accgccgcgc agctgctggc
     2521 caaggagggc gaccacgccc aggtgcgcat gccgtcaggt gaggttcggc gagtcgacgt
     2581 gcgctgcatg gcgacaatcg gccaggtcgg caacctcgat cacgagaacc agtcgctcgg
     2641 caaagccggc cgcagccgcc acatcggccg gcggccgtca gtccgcggcg tggccatgac
     2701 cccgcgcgac cacccccacg gtggcggcga gggcaagtcg cctaccggca tgccgcccaa
     2761 gacgccgtgg ggcaagcctg ccatgggcca tcgcacccgg accaacaagc tgaccggccg
     2821 caacatcgtc cgctcacggc accgcaagag ctaggcgcag gagtccatag atgtcacgtt
     2881 cactaaagaa gggcccctac gtcgaggcgc ggcttctctc gaacgttcag cagatgaaca
     2941 agaagaacga gaagaaggtg ctgaagacct ggtcgcgcgc cagcgtgatc ttccccgact
     3001 tcgtcggcca cacgctcgcg gtctacaacg gcaagaagca catcccggtg tacatcaccg
     3061 agaacatggt cggccaccgc ctcggcgagt tctcgcccac gcgcagcttc cgcggccacg
     3121 gcaagagcac cgagcgctcg agcgcgctga agtaggtcga atgatgaagg tcaaggcaac
     3181 cgccaagttc cagcgcttgt cgacgcgcaa gacgcgactg gtcacccagt cgatcatcgg
     3241 cctgcccgtc gcagaggcga gcgcactact gaagcacatg acccagggcc cggcacgcac
     3301 agtcgcgaag gtgctctcca gcgccgccgc caacgccgag aacaaccatg acctggcacc
     3361 cgacgacctg tacgtcgccg aggccacggc caatgagggc cccacgctca agcgttttca
     3421 cccgcgcgcg cagggccgcg cgtttgcaat tcacaagcca atgacccacg tgaccgtcgt
     3481 ggtcgagaac agggaggcct agcagatggg tcgcaaggtt cacccgtacg gcttccgcct
     3541 tggcacttcc cgcacgtgga acgccaagtg gtacgccgac aagaactaca caacgctgct
     3601 caaggaagac atcacgatcc gccagctggt cgccaagcgc ctggccaatg cgagcgtctc
     3661 gctggtcgac atcgagcgcg gcatcaatca cgtgaccgtg accgttcata ccgccaagcc
     3721 gggcatcgtc atcggcaagg gcggcgcgaa cgtagaggcg ctgcgccagc agatcgggcg
     3781 catcaccacg tccaaggtca agctcgagat caaggagatc cgccagcccg aactcgacgc
     3841 ggttctcgtc gcggccaaca tcgcccagca gctcaaccgg cgcatcgcct tccgcaaggc
     3901 gctcaagcag tcgatccagc gcacgatgaa ggccggcgcc aagggcgtgc gcatcgcggt
     3961 gtcgggccgc ctgggcggtt ccgagatggc ccgtcgcgag tgggacaagg aaggccgcat
     4021 cccgctcaac acgctgcgcg cggacatcag ctacggccag gtccacgccc acacgaccta
     4081 cggccgcatt ggcgtcaagg tctggatcta ccgcggcgac atcatgccca cgcgccaggc
     4141 gccgcccgag gcggcagcgc agggagcggc gtagccatgt tgatgccaaa gcgagtcaag
     4201 catcgccgcg tccagcgcgg ccgcatgtcc ggcctggcca agggcggcta cacgatcgca
     4261 ttcggcgact acgcgctgat gaccgaggag ccggcctgga tcaccgcgcg ccagatcgag
     4321 gccgcacgtc gcgccatgac gcgctacgtc aagcgcggcg gcaagatctg gatccgcgtc
     4381 ttcccggaca agccggtgac caagaagccg gccgaggtgc gcatgggctc cggcaagggc
     4441 gcgcccgacc actgggtcgc ggtcgtcaag ccaggccgga tcctgttcga gatgtcgggc
     4501 gtgccggtca aggaggcatc ggaggcgatg cgcctggccg ccatgaagct gcccgtcaac
     4561 atgaagttcc tgatgcgcga gggggtgggc gatggacatt gatagcgtcc gcaacctgac
     4621 cgacaccgag ctgctcggtg agctggtcac gcagcgccgc gcgctgtatg acctgcgttt
     4681 ccagctggcc acgcgccagc tgaccgatca cagccagctg acccagacgc gccgctccat
     4741 cgcgcgcgtc ctcacggtca tgaccgagcg cagcctcgac gagacgatgg tgccgcgcac
     4801 cgcctcgccc aagcccgctg cccgcgcggc agctgccaag gcggctgcca agtcggccgc
     4861 gaagccggcc gccaagtctg ccggtaaggc gagcacgacc agcaaggaca gcgccgccaa
     4921 gggcgccgcg gccaccgagg ccgcgaccaa gagcactacc ggtgccgaga cgacggccga
     4981 tgccgccaag ggagatgaag agtgagcgca gtcgctcagc gcgccgtgat caagaaggcc
     5041 aaggtgggcc gggtcgtgtc ggacaagatg gacaagacga tcgtcgtgtc cgtcgagcgc
     5101 ctgtcacgcc acccgctgta caagcgcgtc attcgcctct cgaccaagtt caaggcgcac
     5161 gacgagaaca acgaggcgcg catcggtgac accgtgcgca tcgtcgagtc gcgacccttg
     5221 agcgcgacca agcgctggcg catggtcgag atcgtcgctc gcgcaggcga gggcgccaca
     5281 gagccgctcg tctccgagga agaggcgacg agtgaggcca tccacggcgc tgcccacccg
     5341 ggccgccacg ccgccgccgc ggaggccggt gctgaggccg ccgaggaagt tgcgcagtga
     5401 tccagcaata cagccgcctg aaggtggccg acaacaccgg cgcgcgcgtc atccagtgca
     5461 tccgcgtcct gggccgctcc cagaagacga ctgcgggcgt gggtgacgtg atcgtggcca
     5521 gcgtcaagca ggccattcct aatgccgcgg tcaagaaggg cgacgtcgtc caggcggtga
     5581 tcgtgcgcac caccaaggag ctcggccggc cggatggctc atacatccgc ttcgacgaga
     5641 acgccgcggt gctcatcaac aaccagaaca acccgcgtgg cacgcgcatc ttcggcccag
     5701 tggcgcgcga gctgcgcgac cgcggctaca tgaagatcgt gtcgctcgcg ccggaggtgc
     5761 tgtgatggcg cgaacccagc cgccggcgcg cgacatgcgc gtgccggaca tccgcaaggg
     5821 cgaccaggta ctcgtgctga ccggccgcga ggccggcaag aagggcacgg tcgagcacgt
     5881 cgtgcgcaac ggccagggct tcaagaagtc cgtggccaag tacggctcag gctggcgcaa
     5941 ggtcagtccg ctggccagcg ttgccgtggt cgtcgagggc ctgaacatcg ccaaggtcca
     6001 taccaagccg cgccagaagc agggccgcac ggagcgccag ccgcgcatcc agcagggcgg
     6061 catcctcgac gtggcgcagc ccatcctcgc gagcaaggtg atgatcatct gcccgcaatg
     6121 cagccagccc acacggatca agcacggcgt agctggtgac ggccgatcga tccgcctgtg
     6181 caccaactgc ggcgagaccc tgacgaccga aacgcgcaag gagacgcgca agaagtgagc
     6241 gccaaggcgg ccgacgacaa gcccgtgacc aagacgacga agacaacggc gaaagccgag
     6301 ccaaaggcca ccgccaaggc gccggcagca gcggccaggg cgacggcaaa ggcgccgtcg
     6361 aaggcagttg ccagggccga gccgaaggct gcccccaagg cgccgccgaa ggccgcgagc
     6421 aaggctccgg cgaaggccgc cgccgagccc agggctgcgg ctatggtgga ggctccgtcg
     6481 ccggctccgg aagccaagat acagcgtggc gcggcgaagg ccgcacccgc cgctgccaac
     6541 ggcgcactgc acggcctgca cctgcgctac cgcgacgagg tcgcgccggc gctccagaag
     6601 gagttcagct actccaaccc gatgcaggtg ccgcgcctcg acaagatcgt ggtgaacatc
     6661 ggcctgggcg aggcgctgac caacgccaag gcgctcgatg ccgcggtggg cgacctggcc
     6721 gcgatcaccg gccagaagcc gatcgtgacc cgcgcgcggc gctcgatcgc ccagttccgg
     6781 gtccgcaccg gcaatcccat cggcgccaag gtcacgcttc gcggccagcg catgtgggac
     6841 ttcctggagc ggttgactcg cctggcgctg ccgcgtatcc gcgacttccg gggagtgccg
     6901 gacaagtcgt tcgacggccg cggcaattac tctctcgggc tgcgcgaaca gctcgcgttc
     6961 ccggagatcg actacgacaa gatcgacaag ctgcgcggcc tcgaggtgag catcgtcacc
     7021 accgcgcgca ccgacgaaga gtccaagcgg ctgctgcagc tgctcggcat gccattcgcc
     7081 aactagtcaa gcactagcag cgacgccgag cagaacgggc agagaaaccg agaaagggtc
     7141 caatggcaaa gaagtcactc atcgcgaagg ccaagcgcaa gccgcaccag acgtcgcagg
     7201 gctacaaccg ctgcttcgtg tgcggccggc cgcgcggcta catgcgccgt ttcggcctgt
     7261 gccgcatctg cttccgcgag cgcgcgctgc tgggcgagct gcccggcgtg accaagtcga
     7321 gctggtagcc agatgaacat gtcagacccc atcgcagaca tgctgacgcg catccgcaac
     7381 ggcagtcgcg cgcgccattc ggagatcctc gtgcccgcgt cgcggaccaa gcgcgcgatc
     7441 gcccagatcc tgtcagacga gggcttcatc gacggcttcg aggacgccca ggatggcaac
     7501 caccagcagc tcaagctgca cctcaagtac gtcggcaagg tgccggtcat cagcggcatg
     7561 aagcgcgtca gcaagcctgg cctgcgcgtg tacgcgacca agaccgacat cccgcgcgtg
     7621 ctcggcggcc tgggtgtcgt catcgtcagc accagccacg gcatcatgac cggcgcccgc
     7681 gcccgacagg cgcagctggg cggcgaagtc ctggcctacg tctggtaatc ggtagcagcg
     7741 gaagtcggta gctagcaatg tcacgaatcg gcagactcgc catacccatc ccggccggtg
     7801 tcacggtcga cgttgagggc agccacgtcg cggtcaaggg ccccaagggc atgctcgagc
     7861 gcgacctgtc gcccgagctg cgcatcgtca aggacgacgg cacgctgcgt gtcgagcggc
     7921 ccaacgacga gaagaagtcg cgcgagctgc acggcctgac acgaaccctg ctcaacaaca
     7981 tggtcatcgg cgtgaccgag ggcttccgca agccgctcga gatcaccggt gtcggttatc
     8041 gcgcccagct cgtgggcaag aagctgcagc tcaacctggg ctactcgcac cccatcgaga
     8101 tcgatccccc tgcgggcatc agcttcgatg tcgagtcgcc catcaagctc gccgtcgtcg
     8161 gcatcgacaa ggagctcgtc ggccacatcg ccgcgcgcat ccgctcgacg cgcaagcccg
     8221 agccgtacaa gggcaagggc gtgcgctacg ccggtgaggt catccgccgc aaggccggca
     8281 aggcgggcaa gatcggaggc aagaagtgag cctcgctact tcacgcggca cgtcgcgcca
     8341 caagcgccac gcccgtctgc gcctgaccct ctccggctcg cctgagcggc cgcgcctggc
     8401 ggtcttccgc agcatcaacc agatctacgc ccagctgatc gacgatacgg ccggcaagac
     8461 gctggccagc gcctcgtcac tggaaggcgg cctgcgcgat cgcaagggca ccaagaccga
     8521 agacgccaag caggtcggca agctgctcgc cgaacgcgct cgcgcggccg gcgtcacgaa
     8581 ggtcgtgttc gaccgcgccg gcttccgtta tcacggccgc gtgcgcgccc tggccgacgc
     8641 cgcgcgcgaa gccggcctcg acttctaacc aggagaaaga ccaagtcatg ccgaggatcg
     8701 atcccgcccg tctcacgctc gaggagcgtg tcgttcagat caaccgcgtt gccaaggtgg
     8761 tcaagggcgg ccggcgcttc tcgttcagcg cggtcgtcgt tgtcggcgac ggcgccggcc
     8821 atgtcggcgt tggcctcggc aaggcgggcg aggtgccgga ggccatccgc aagggcgtcg
     8881 aggacgcgaa gaagcatctg atcaaggtgc cgctcgtcgg cacgaccatc ccccacgccg
     8941 tgacccagca gtacggcgcg tcgacggtga tgctgaagcc cgcctcacag ggcaccggcg
     9001 tcatcgccgg cggctccgtc cgtgcggtgg tcatcgccgc gggcatccgc gacatcctca
     9061 gcaagtcgct cggctcgacc aacccggtca acgtcgtgcg cgccacccac gaggcgttgc
     9121 gcggcatgag cagcgccgag gagatcgggg cgcgccgcgg caagaagctg cgcagcgtca
     9181 tcagcggcca gccgggcgcc ggcccgcgcg cggaggcaac tgccaatgcc ggctaagctc
     9241 aaggtcacgc tcatcaagag ccccatcagc cacacccagc gcacgcgggc gaccatgagc
     9301 gccctgggcc tgcatcgcat cggccagtcg gtcgtgatcg atgacacgcc cgagatgcgc
     9361 ggcatgacgc gcgccatccg attcctgctg cgcacggagc cgaccgatgg cgcggagaca
     9421 gccgcgaagg ccaagtccgc ggcgcgcaag aaggaagcga gcaagtcgtg aagctgcatg
     9481 acctgcgccc ggccgccggt gcgcgcaagg cgcgcacccg tgttggccgc ggcatcgccg
     9541 ccggccaggg caagaccgcc ggccgcggca ccaagggcca gcgctcgcgc gcgggcgcga
     9601 gcatccccgc ctggttcgag ggcggccaga cgcccatcca tatccgcgtg cccaagctgc
     9661 acggcttcaa gaacaagttc cgcgtccagt acgcggtggt caacgtcggt cagatctcgg
     9721 cctacgccga ggccggccgg ctgggtgacg ccaagccgcc caccgtcaac gccgagacgc
     9781 tcgcccaggc gggcctgatc cgcggcgacg gccggccgct caaggtcctg ggccagggcg
     9841 aggtcgcgac caagctgttt gtcgcggccg acgcgttcag cgccagcgcc aagacgaaga
     9901 tcgaggccgc cggcggcaac gtccttgtcc tagccgagcc caagccggag aaggttgcga
     9961 agcctgataa agccgacaag tcggccaaga aggccaagcc ggctcaggcc gatgcagggg
    10021 aggaggccaa cgggtcggag tctccgaagg ccgaatcgga gccccagcct gaagcagccg
    10081 ccgaagtcag cgactcgtct gaatccactg agtccgacaa ctaagacccg acaggggatc
    10141 tataaaagat gattgaagcg ctgatcaacg cgttccgcgc gccggacatc cggcggcgca
    10201 tcctgttcgt gctggtgatg ctggtcgtgt accggctgct cgcggtcgtg ccggtgccga
    10261 acgtcaacca ccaggcgctc gatcagctca tcaacaacca gacgttcctg cagctgctcg
    10321 acctgttctc gggcggcggc ctgcgctcgt tctccatcgt ggccatgggc gtgaacccgt
    10381 acatcaacgc ctcgatcatc atgcagctca tgcagggcgt tgtgccgcgc ctgcaggcgc
    10441 tctcacgcga gggcgagtac ggccggacgc ggctcaatca atacacgcgc tacctgaccg
    10501 tgccgctggc gctgctccag gcctatggct acctggccat cctgtcgcag agccagatcg
    10561 tcgagccgat cgacatcctc agcccgacca cgttgaccca gatgatcacc ctcgcgggcg
    10621 gcaccctgct cgccatgtgg atcggcgagc taatcaccga gcgcggcatc ggcaacggca
    10681 tcagcttcct gatcttcgcc ggcatcgtcg cccgcatccc gcagggcgtg gccgcgttcc
    10741 tccaggcgcc cgacatcccg gccggcatcc tgttcgtgat cctggccctg gtggtcgtgg
    10801 cggtgatcgt ctacatccag gaagggcagc gccgcatccc catccagtac gcgtcgcgcg
    10861 ttcgcggccg gcgcatgtac cagggtggcg cgaccttcct gccactgcgc gtcaaccagg
    10921 ccggcgtcat cccgatcatc ttcgcggtga gcatcctgct gttcccgacg cagatcgcgt
    10981 cgtacttcgc gacgccgccg aacgccgaac cgtccctcgt caactccata gccgacggca
    11041 tcgtcggctt cctgagcccg aacggcatcc cctacatggt cttctacttc ctgctgacca
    11101 tgggcttcac gtacttctac accgcgttca ccttcaagcc cgatgagacc gccgatcagc
    11161 tgcgcaagaa cgggggcttt atccccggca tcagacccgg cgcgcccacg cgcgactacc
    11221 tgcagaaggt cgtcttccgg ctgagcctcg cgggcgccat cttcctcgcc ctgatcgccg
    11281 tttcgccgat cgtgctctcg tcgctgttcg ctggtcaggg ggtggtcggc ggcttcgcgc
    11341 tgggcggcac tgcgctgctc atcgtcgtga gcgtggtggt cgagacgatg aaacagatcg
    11401 aggcgcagct gatgatgcgc aactacgagg gcttcattcg ttgagggtgt atgtcatggt
    11461 cggggcgccg ggtgccggca agggcacgca ggcggcgctc ctgtccgagc ggctggggat
    11521 gccccatgtc gcgtcgggcg atctcttccg tgacaacatc aagcgcggca ccgctctcgg
    11581 caagaaggtc aagggctatc tcgagtcggg cgcgctcgtg cccgactccg tcacggtcca
    11641 gatgatctcc gaccgcctgg ccaagcccga cgcggccgat ggcgcaatcc tcgacggctt
    11701 cccccgcacc cggccccagg cggaggcgct cgatgccatg ctcgccaaga tggacggcga
    11761 ggtcggcgcg gcgctgtatg tcgacgtggc gcgcgacgag ctgctgcgcc ggctgtcggg
    11821 gcgctggatc tgcacggtca gcccggatca cgtctaccac tcggtagcgc gcccgcccaa
    11881 ggtcgagggc gtgtgcgata tcgagggcgc gccgctctac cagcgcgacg acgacaagcc
    11941 ggccacggtc cagacgcggc tggagcagca gctgccgccg atgtacgagg tggtcgacta
    12001 ctacaccgat cgcggggtgc tgagcaccgt tgacggcgcg ggcgaggtcg cgcaggtgac
    12061 cgacgccctg ctgcacgcca tcgcccagcc ggcgcgcctg cagggctaga cgcatgacac
    12121 ccgaccggcg ggtaacgctc aagagcagcg cccagattga gaggatggcg atcgccggcg
    12181 cgctcgtcgc gtccgtgctc gaccgcctcg cggccgaggt gaagccgggc gtgaccacgc
    12241 tccagctcga cgccatcgcc gaggagatga ttcgagccgc gcatggcgtg ccgtcgtttg
    12301 tcggcgtgcc cggccgcgtc gcgccctatc gccacacgct gtgcgtctcg ctcgacgacg
    12361 agatcgtcca tggcgtgccc ggcccgcgcc gcatccgcga aggccagatc gtgtccgtcg
    12421 acgccggggc cgtcgttgat ggctggcacg gcgacgcggc acgcacgttc atcgtgggcg
    12481 aggtgccgga ccacacgcgg cgacttgtcg agacgacgga acgagccatg tacgcgggca
    12541 tcgcggcggc ccagccgggg gccttcctgg ccgacatctc gggtgcgatc gaggacatcg
    12601 gtcgcgacgc cgcctacggc gtcatccgcg cgttcgtggg ccatggcatc ggcacggaga
    12661 tgcacgagga gccgcaggtc accaactacc gcacgggctc ccgtggccga cgcatcgaac
    12721 ccgggctgtg cctcgcgatc gagccgatgt tcacgctggg cggccacgag gtgcggatcc
    12781 gcgaggacgg ctggacggtc gtcacggccg acggctcact ggccgcgcac tgggagaaca
    12841 cgatcgcggt gaccgcggat gggccgcgcg tcctgaccgc gtccgcgccg gcgttcatgc
    12901 cggtggcgag cggagccccc gaggcgttgc cgacgaggtg acacttctgg tacactcccc
    12961 gttcgtgcgt cagactccgt ctggcgcgtc accacgctcg gtggctgggt cattctcggc
    13021 cgcgaatccg ggccccattc accgtcagtt tcagttgaag gagcctgccg ccgcctgtgg
    13081 ccaagaagga cgcaatcgaa gtcgagggta cggtcatcga gccgttgccc aacgcgatgt
    13141 tcgccatcga gatcgatgcc gcggacaaag tgaacaaaca ccgcgtcctg gcgcacatca
    13201 gcggcaagct gcgcatgaat ttcatccgca tcctgcccgg cgatcgggtg cgcgtggagc
    13261 tgtcgccata cgacctctcg cgcggccgca tcacctatcg cctcaagtag aaggactctc
    13321 gtgaaggtct cggcatcagt caagacgcgc tgcgataact gcaagatcat ccgtcgccac
    13381 ggcgtcgtga tggtcatctg cacgaatccc aagcacaagc agcgccaggg ctagggcaag
    13441 cagatggcac gcatctccgg catcgacatc ccgcgcgaga agcgcgtcga ggtcgccctg
    13501 acgtacatct acggcatcgg cctgccgacc agccagaaga tcctcgccca ggcgaacgtc
    13561 aacggcgaca cccgcgtgcg cgacctgacc gaagaccagg tcaaccggct gcgcgagatc
    13621 atcgatcggc gctacaaggt cgaaggtgac ctgcgccgcg aggtcgcgct gaacatcaag
    13681 cgactccagg agatcggctc gtatcgcggc ctgcgccacc ggcgcaacct gcccgcgcgc
    13741 ggccagcgaa ccaagaccaa cgcacgtcag cgtcgcggac cgcggcggac tgtcggcgtc
    13801 aagcgcaaga agtagggagc agccacagcc tcatggcgga gaagaagggc gcgaagaaga
    13861 cgcgccggcg cgagaagaag aacgtgccgg ccggccacgc gcacatccag gccagcttca
    13921 ataacacgat catcaccatc accgacctcg ccgggggcac ggtctcgtgg ggcagcgccg
    13981 gcgtggccgg cttcaagggc tcgcgcaaga gcacgcctta cgccgcggcc ctgtccgcag
    14041 agggcgcggc gcgcaaggcc atggagcacg gcgtgcgcca ggtcgaggtt ttcgtcaagg
    14101 gccccggcgc gggtcgcgag caggccatcc gctcgctcca ggccgccggc atcgaggtga
    14161 ccgccatcac cgacgtgacc cccattccac acaacggcaa ccgcccgccc aagcggcgcc
    14221 gggtctaggg ggagcgacaa gagatgtcac gttaccgcgg tcccgtttcg cgcctgtgcc
    14281 ggcgcgaggg catgaagctc ttcctgaagg gcgccaagtg ctacaccaag aagtgcccct
    14341 tcgagcgccg tcccaatccg cccggccagc acggcgtgcg ccggcgcaag gtgtcggact
    14401 tcggcctgca gctgcgcgag aagcagaaga tgcgccgcat ctacagcgtc cacgagcgcc
    14461 aattccgaaa ctacttcaac gaggctgagc gccacacggg tgtgaccggc gaggcgctgc
    14521 tgcgcatgct cgagacgcgc ctcgacaacg tcgtcttccg aatgggcttc tcgacgagcc
    14581 gcgcccaggc gcgccagctc gtgtcgcacg gccatttcat ggtgaacggc accgccacga
    14641 acatcccctc gtacgcggtc gccgagggcg accgggtcga ggtgcgcgag gcgagccgca
    14701 agtccgagta cttcaagaac gcgcgtgaaa cgctgaaggg tgcgcagcgc cccgattggc
    14761 tgtcggttga ccccgacaag ctgagcggca gcgtcaacgc ccagccgcgg cgcgaccaga
    14821 tgccggcgga gctgaacgag cagctggtcg tcgagttcta ctcgaggtag ttacgcgggc
    14881 gccaggcctg cttaggagaa gcatccagtg attgagctag agaacccaca gatcgaggcc
    14941 gtcggcgaga cgcaggactg gtacgccaag tacgaggcca gcccgctccc cgccggctac
    15001 ggcgtgacga tcggcaacgc gctgcggcgc gtcctgctct cgagcctcga gggcgccgcc
    15061 gtgacttcca tccaggtgcg cgacgtctac cacgagttca gcaccatccc gggcgtcgtc
    15121 gaggacgtga ccgcgatcgt gctcaacgtc aaaaagctgc gccttaagag ctatgcgccc
    15181 cacccggtgc agctgcgcct gtccaagtcc ggtgccggcg cggtgaccgc gcgcgacatc
    15241 gaggccaacg cggacgtcga gatcatcaac cccgaccagc acctgctgac ccttgactcg
    15301 gacgatgcgc cggtcgagat cgacatgacc gtcgagcgcg gcgtgggcta cctcgccgcg
    15361 gagcgcagcg aacagctgcc gatcggtgtc atcccggtcg acgccatctt ctcgccggtg
    15421 cgcaaggtca actacacggt cgagaacacg cgcgtcggcc agatgaccaa ctacgacaag
    15481 ctgaccatcg agatcgagac cgacggcacg ctcacgccag aagaggcgct cagccgcgcc
    15541 gcggagattc tggtcggcca gttcagcctg ttcaccgcgg cgggcaagca gatggccgcg
    15601 acggagagcg gcctgcccgg cgcgccgcag ctgccgccca acatgctcga catgcccatc
    15661 gaagagctcg acctgccgat gcgcgcctac aactcgctga agcgcaacaa catcgtcaag
    15721 gtcggccagc tgctgcagct ccaggacgag gatctgctgc ggatgcgcaa cttcggcaag
    15781 aagtcgctcg acgagatgaa ggagcgcctg cgcatgcgcg ggttcctgcc gcccgacgaa
    15841 ggtggcgacg cggccgagtt cgatcccgag tcgggtctcg acgacctgga cgaggaagag
    15901 taggcaaatg gcgcactgca tagcgggccg caagctcggt cgcaagaccg ggccgcgcaa
    15961 ggcgctgttc aagaacctga tcgtcgccgt gctgcgctat gagcagatca agacgaccga
    16021 ggcccgcgcc aaggaaatcc gcggtcaggt cgagcatgtc atcaccctcg ccaaggacgg
    16081 ctcactggcc gcgcggcgcc agatcatcgc ccagctgccc gacgagccgc tcgtcatcga
    16141 caagctgatc aacgagatcg cgcccaagta cagcgatcgc acctcgggct acacgcgcat
    16201 catcaagatc gggccgcgca acggagacgc ggcaccgatg gtccagctag agctggtcta
    16261 gctagcagac gccagcccgc atgggcggca caggcaagag cggtcgcaag gccgcgccac
    16321 ggaagtgcgc tcgagagggc gtccccgtgc ggtaccgcgc gcgggtcgaa tacgacggca
    16381 ctgactttgc cggcttccag gttcaacctg gaggtcgcac ggtccagggt gagctggaaa
    16441 gcgcgctgcg tcgactcgcg ggtgggaccc gggtggtcgt cgacggctct ggacgcaccg
    16501 acgccggagt tcatgcccgc gggcaggtga tcgctttcac ctacaccggg cgactcggtc
    16561 ggtcggagct ccagaaagca ctcgcggcag gcctgccgag ggacatcgga atcggtccga
    16621 tcatcccggt cgggcaggac ttcagcccac gtcatcaggc caggcaccgg gaataccgct
    16681 acttgatctg gaacggacgg cgcagcccgc tgcgtgaacg ccaatcgctt ggcgtgaggg
    16741 agcggctcga tgtcgcggcg atggccgcgg cggcaaaggt gttcatcggc cggcacgact
    16801 tctccgcctt tggcggggcg gatcgccagc cggtgcggac gctgaccaag ctgagcgttg
    16861 tccgccgggg cgacctgatc accgtgacgg tgatcggcga cgcgttcctg cggggcatgg
    16921 tgcggcgcat cgtggccgct ctgctgagag tgggtcacgg acgggcaagt gccgaggatc
    16981 tggcgcaggc gctgggcgaa cgacgcccgg cgttcaacgg cgaggccgcc gcgccacgcg
    17041 ggctgaccct ctggcgcgtg ccgatagggc cccgccgcca caagacacga aaaagggaag
    17101 agcaggacaa tcaggaatga tgagcaggac gtacagcccc agggctagcg agatcgatcg
    17161 ccactggttc gtggttgacg ccaccaacca gcgcctcggc ctgctcgcgt cacggattgc
    17221 cttcgtgctc gagggcaagc acaagcccac ctacgccacc cacatcgata ccggcgatca
    17281 cgtcatcgtg ctcaacgccg cgaagatcga cgtcggcggc aagaagagcg agcagaagac
    17341 ctactcacgc cactcgggct atccgggcgg ctaccgcgag gagaccctcg ccgacctgct
    17401 cacgcggcga cccgaagagg tcatccgccg cgccgtcaag ggcatgctgc cgcgcaaccg
    17461 actgggcgtg cagcagctgc gcaagctgaa gatctacgcg ggcaccgaac acccgcacga
    17521 ggcgcagcag cctgagccgc tgccaatggg aggttcgact cgctgatggc tgccgggacc
    17581 acatccttct tctacggcac gggccggcgc aagaccagca tcgcgcgcgt gcgcctgctc
    17641 gccggtgagg gcgagatcgt cgtcaacggc cgctccctcg aggagcactt cggcaacgcc
    17701 atcgacctga ccgaggtcac gatgcctttc aaggtgacca acaccgaagg tcgctacaac
    17761 gccatgatca aggtcgaggg cggtggccac cacggccagg ccggcgcgat ccgccacggc
    17821 atcgcccgcg ccctgctcca gtcggacccg gaaggcgttc gtgccccgct ccgctcggcc
    17881 ggcctcctga cgcgcgaccc gcgcatgaag gagcgcaaga agttcggcct caagagggcc
    17941 cgcaaggccc cgcaatacac gaagagatag cctccgcagc ggccctcacg ggccctgctg
    18001 cgcgtcgtcg gtgaccccgc tccggaggag cgtggacgcc tcctccttgc gggcgctgag
    18061 cgggggccgc ctcatgcggc cacgctgggg cgttgccgcc cacgttcgct cgcttacgca
    18121 cgagagtgcg cgcgctcgct agcttgccct ttccgtcgat tctcgccatt cgctcgtccg
    18181 cggccgcgtc ctggtcagtt tctcgccatt tgcgcgataa ggacctaacc aagcggtttg
    18241 gccagccccg ggctgcgtat tgccgagatt cggcagaaaa accgcctcgt tggcaagcac
    18301 ggacgtcagg cagaccgtag gtgcggtgaa cgcaccgaac tgacatctgg cgggcctggc
    18361 ctagcccggt cgcgccatat cgggcgcacc cggagtggcc cgatgatcgg cccgtggtag
    18421 acgtttacgg caagtacgcg gaaacgctgc gcgccggtca ccagttcgcg gcctcagggc
    18481 agctcaagga agcgctgcgt tgctatcgcg cggcgaccga gctcgccggc gagcgcgccc
    18541 tgccgcacgt cgcgctcggc ggcacgctcc tgcgcctgaa ccagccgcgc gaggcgctcg
    18601 cggcatatga gcgcgcgctc gagctcgaac cggatcagat cgacgcgctc acaggacgcg
    18661 cggcggctct gctggcagcc ggccggcgca ccgaggcggc gcaggtccag gagcggatca
    18721 ggcaaggccc cggaagcggc cccaacagcg cggccggcct cgccacgccc ggttccagcg
    18781 cggaccgcta cctggcgagc ggcgagcagg cgcgcgcggc aggcaagacc gacgccgcga
    18841 tcgacgcctg gctggccgag tcggcagagc atcgcgtcgc cggccaccac gacgcggccc
    18901 tcgacgcctg cctgcgcgcg ctggcgctcg actcgtcggc gccacgtacc cacctcgaga
    18961 tcgcgcgcgc ctggctggcc cgtggctggc ccgaccgcgc cgcccagcgg ttgcagctgc
    19021 tggagcgtct gctcgtcctc cagcccgaca aggaggtggc cgccgccgcg cgggagctct
    19081 tcgccggctg acgcagggcc gaggcccgct cacgcaggac cggtggcccg ctgacgcagg
    19141 accggtgagc cgctggctac cattgccgcg atggacgcgc tcctcgaact ggtcaaaagc
    19201 ctccaggttg agccgatcgc atttctggat atcggcttga ccgcgctgct gatctacggc
    19261 ctgttcagcc tgattcgggg cacccgcgcc gtccgtctcg tgattggcgt gaccgtgctg
    19321 tacgtcatct acgtgcttgc ccaggcattc ggcctgcagc tcctgtcgca gatcctccag
    19381 gcgggcgccg ttgtcggctt gctcgccctg gtggtcgtgt tccagcccga gctgcgccgg
    19441 gcgctcgagc gcatcggccg cttcggttcg atgggctggc tgctcgcacc aggcgctcgt
    19501 gcgtcgtcgg agcgggtcgg cgcagtagtc gccaaggccg ccgtcgcgct gggcgcgcgc
    19561 aagaacggcg cgctgatcgt gatcgagcgc gagaccggtc tgaccgacgc cgcggagacg
    19621 ggcatcatgc tcgacgcgca gctgacggtg gagttgctcg agactatctt cacgccgcac
    19681 tcggcgctgc acgacggcgc ggcgatcgtg cgccacgaaa gagtcgtgtc cgcgggcgtc
    19741 gtcctgccgt tgagcgagag cggcgtctat cgcgagcgct tcggcacgcg ccaccgcgcg
    19801 gcgctgggga tcaccgagca gacggacgcg atcgctgtgg tggtcagcga ggagacgggt
    19861 agcgtcagcc tcgta
//
LOCUS       DATBRX010000080         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1812854, whole genome shotgun sequence.
ACCESSION   DATBRX010000080 DATBRX010000000
VERSION     DATBRX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812854"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..796)
                     /locus_tag="VIF08_02995"
     CDS             complement(152..796)
                     /locus_tag="VIF08_02995"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY8134981.1"
                     /translation="MSATSPDGLAPLRPLERPFFDRATAEVARDLLGVWVVRSTAEGL
                     SGGPIVETEAYGGPEDLASHARAGLTRRTSPMFGEVGHAYVYLVYGMHECLNVVAHNG
                     AEAGAVLIRAISVDLGMDQVRQRRGRPTDPINRLCSGPAMVCQGLGVDRSFDGHDLTL
                     GEGLWLASGSGRESLDDIANGPRIGVDYAGEWAARPWRFWIAGHPSVSRPARRP"
     gene            complement(777..1316)
                     /locus_tag="VIF08_03000"
     CDS             complement(777..1316)
                     /locus_tag="VIF08_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY8134982.1"
                     /translation="MLADIAIIVLLVLFLLIGFFRGALRQLLALGAWLIALVVAAHGR
                     GFLADWLLAQEPDFSVQYANMLGFLVSFLILLVAALAIIEFSGRTVTLSNRAMVEELV
                     GGAALLLVGVLAITGVLFVLDTYYAAPSGFTAEVDLVRQLHTALTESKIATALHDTLV
                     PLVQSLLGPLLPPDVRHFA"
     gene            complement(1306..>1557)
                     /locus_tag="VIF08_03005"
     CDS             complement(1306..>1557)
                     /locus_tag="VIF08_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134983.1"
                     /translation="VGRDTPAANVEAAIRSNAGPNLASVTLFDRYTGAPLAEDEVSLA
                     YRLRFQPGAEQLSEAQLDEAIARVTKALEREVKGRIRAG"
BASE COUNT          277 a          541 c          534 g          205 t
ORIGIN      
        1 tcggtctcgt cgagccatct cctcacgacg agtggctcgg acgacggctc gatcgcctcc
       61 gccaaccgtc gcgacggcgc gaacgcggtc agcgccgcga ggcgcgcgcg gatctgtggg
      121 aactcgagca gctcctggct gcgcgcgtcg gtcatggccg ccgggcgggg cgcgaaacgg
      181 acgggtgccc ggcgatccag aagcgccacg gccgcgcagc ccactcgccg gcatagtcga
      241 cgccgattcg cggcccgttg gcgatgtcgt caagcgactc acgaccggac cccgacgcga
      301 gccacagccc ctcccccagg gtcagatcgt ggccgtcgaa tgaccgatcg acccccaagc
      361 cctggcagac catggccgga ccggagcaca ggcggttgat cggatcggtc ggccgtcccc
      421 gccgttgacg aacctggtcc atgcccaggt cgacagagat ggcgcgaatc aggacagccc
      481 cggcttcggc accgttatgg gcgacgacgt tcaaacactc gtgcatgccg tagacaaggt
      541 agacgtatgc gtggccaacc tcgccgaaca tcggcgacgt gcgccgcgtc aggccggcgc
      601 gcgcgtgcga cgcgaggtcc tcagggccgc catatgcctc tgtctcgacg atgggcccgc
      661 cgctcagccc ctccgccgtg gaacgcacga cccagacgcc gagcaggtcg cgcgcaactt
      721 cggcggtcgc tctgtcgaag aagggccgct ccagcggtct gagcggcgcg aggccgtcag
      781 gcgaagtggc ggacatcggg cggcagcagc ggcccgagca gcgactggac cagcggcacc
      841 agcgtgtcgt gcagcgccgt ggcgatcttg gactcggtca gcgcggtgtg gagctggcgc
      901 accaggtcga cctcggcagt gaacccggag ggagcggcgt agtaggtgtc aagcacgaac
      961 agcaccccgg ttatcgcgag tacgccgacc agcaggagcg cggcaccccc cacgagctcc
     1021 tcgaccatcg cccggttcga cagggtcacc gtgcggccgc tgaactcaat gatcgccagc
     1081 gcggcgacaa gcaggatcag gaagctgacc aggaagccga gcatgttggc gtactggacc
     1141 gagaagtcgg gctcctgcgc gagcagccaa tccgccagga agccgcgccc gtgggctgca
     1201 acgaccaatg cgatcagcca cgccccaagg gccagcagct ggcgcagcgc gccgcggaag
     1261 aagccgatga gcaggaacag gaccagcaga acgatgatcg cgatgtcagc cagcacggat
     1321 ccgccccttt acttctctct ccagcgcctt ggtgacccgg gcgatcgcct cgtcgagctg
     1381 agcctcgctc agctgctcgg cgcccggctg gaagcgcaaa cggtaggcga ggctgacctc
     1441 gtcctctgcg agcggcgctc cggtgtagcg atcgaacagc gtgacactcg cgaggttggg
     1501 cccggcattt gaccggatcg cggcctcgac attcgcggcg ggcgtgtcgc ggccaac
//
LOCUS       DATBRX010000081         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1924608, whole genome shotgun sequence.
ACCESSION   DATBRX010000081 DATBRX010000000
VERSION     DATBRX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924608"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VIF08_03010"
     CDS             <1..906
                     /locus_tag="VIF08_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8134984.1"
                     /translation="IGAGSGALDVAMNAAVSDVEVSSGKRLMYGAHALFSLAVLVASV
                     ATGFARQAGAGPLPILAVVAVLIALTAISTIASARARHRPIVTAPTTSISAAGHHPAA
                     VLGGLATLAILCAVGYLIEDATQNWSALHLERSLGASPALGGAAPGIFAGAMFIGRSS
                     GQRLGARFSERSLFASGAALAALGLAILAAAPSAVVALVGLAATGAGIALVAPALYGR
                     AGRAAAANDRGKAIARLTIFGYTGFIVGPALIGFISQAASLRAGIGTLAVLAAGLAVG
                     GWFALRGRPRATFEEGEELLKTSRA"
     gene            942..1373
                     /locus_tag="VIF08_03015"
     CDS             942..1373
                     /locus_tag="VIF08_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEY8134985.1"
                     /translation="MQFQQFDHRYQLRFMAGEKAVATLLDFCRREGIGYAALSGLGAV
                     SAVDLAYFNDETQKYETHEIAEQLEVIGLTGNITIKDGAPFAHVHATFGDRDLVVRGG
                     HVMEMTVKPNLEIWLTRGTQDVVRLPDEESGLYLMSLPDKT"
     gene            1370..>2073
                     /locus_tag="VIF08_03020"
     CDS             1370..>2073
                     /locus_tag="VIF08_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY8134986.1"
                     /translation="MNEPSGRVVQVNVSGGGVPKGPVERAWVGVLGLEGDAHRENTVH
                     GGPHRAVCLFGIEVIERLQSEGHPIEPGGAGENLTTSGIEWSLLPVGTRARIGEQLEL
                     EVSSSTTPCKTQVANFSDGNFNRIRIEQHPSDSRMYARVITEGEVKPGDPITLLPDVN
                     PEAAIDEMLRRLHRAETKSAVAAWRAAAASGFAVDILEDGEIALASSTDLPGPAFNSG
                     LGLVRLPNMLSMATDF"
BASE COUNT          323 a          701 c          723 g          326 t
ORIGIN      
        1 atcggcgcgg gatctggcgc gttggatgtc gcgatgaacg ccgcggtcag cgacgtggaa
       61 gtgtcgagcg gcaaacggct gatgtacggc gcgcacgcgc tgttctcgct ggccgtccta
      121 gtggcgagcg tcgccaccgg atttgcccgc caggcaggcg ccgggccgct gcccatcctg
      181 gcggtagtcg cggtcttgat cgcgctgacg gcgatttcga cgatcgccag cgcacgcgcc
      241 aggcaccgac caatagtcac ggcaccgacc accagcatca gtgccgcagg ccaccaccca
      301 gctgcggtcc tgggcgggct ggccacgctc gcgatcctgt gcgccgtggg ctacctgatc
      361 gaggacgcga cccagaactg gagcgcgctg catctcgagc gatcgctggg tgcgagcccg
      421 gcgctcggcg gggccgcgcc gggcatcttc gccggggcga tgttcatcgg ccgcagctcc
      481 ggccagagat tgggcgcgcg attcagtgag cggtcgctct tcgcttctgg cgccgcgctc
      541 gcggcgctgg gtctggcgat cctcgccgcc gccccgtccg ccgttgttgc gctcgtcggt
      601 ttggccgcga ccggcgctgg catcgccctg gtcgcgcccg cgctctacgg acgcgccggc
      661 cgagcggccg cggccaacga tcgtggcaag gcgatcgcgc ggctgacgat cttcggctac
      721 acgggcttca tcgttggccc agcgctgatc ggcttcatct cacaggcagc cagcctgcgc
      781 gcgggaatcg gcacgctggc ggtattggca gcagggctgg cagtcggtgg ctggttcgcg
      841 ttgcgcggcc gtccacgggc cacgttcgaa gaaggcgagg agctgctcaa gaccagccgc
      901 gcctgacgcg cagcgctcgc cacaccggat atcgtcgcgc catgcagttt cagcagttcg
      961 accatcgcta ccagctgcgc ttcatggcgg gcgagaaggc cgtggccacg ctgctcgact
     1021 tctgccggcg cgagggcatc ggctacgcgg cgctgtccgg cctgggagcg gtgagcgccg
     1081 ttgacctcgc ctacttcaac gacgagacgc agaagtacga gacgcatgag atcgccgagc
     1141 agctcgaggt gatcgggctg accggcaaca tcacgatcaa ggacggcgcg ccgttcgccc
     1201 acgtccatgc cacgttcggc gaccgcgacc tggttgtcag gggcggccac gtcatggaga
     1261 tgaccgtcaa gcccaacctc gagatctggc tgacgcgcgg cacgcaagac gtcgtgcggc
     1321 tgcccgacga ggagagcggc ctgtacctga tgtcgctccc ggacaagacg tgaacgaacc
     1381 gagcggccgc gtcgtgcagg tcaacgtgtc gggcggcggc gtgcccaagg gcccggtcga
     1441 gcgcgcctgg gtgggcgtgc tcggcctgga gggcgacgcc caccgcgaga acacggtgca
     1501 cggtggaccg caccgcgcgg tatgcctttt cggtatcgaa gtcatcgagc gtctccagtc
     1561 ggaaggtcac cccatcgagc ccggcggcgc gggcgagaac ctgacgacga gcggcatcga
     1621 atggtcgctg ctgccggtgg gcacgagggc gcgcattggc gagcagctcg agctggaggt
     1681 ctcgtcctcg acaacgccct gcaagacaca ggtggccaac ttcagcgacg gcaacttcaa
     1741 ccggatccgg atcgagcagc acccgtctga cagtcggatg tacgcacgcg tgatcaccga
     1801 gggcgaggtc aagccgggcg acccgatcac gctactgccg gacgtcaacc ctgaagcagc
     1861 aattgacgag atgctgcgcc gtctccaccg ggcggagacg aagtccgctg ttgctgcctg
     1921 gcgcgctgcc gcagcgtcag gtttcgcggt cgacatcctc gaggacggcg agattgccct
     1981 ggcgtcatcg accgacctgc ccggtccggc cttcaactcg ggcctgggcc tcgttcgctt
     2041 gcccaacatg ctgtcgatgg cgaccgactt ctt
//
LOCUS       DATBRX010000082         7388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2259109, whole genome shotgun sequence.
ACCESSION   DATBRX010000082 DATBRX010000000
VERSION     DATBRX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2259109"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VIF08_03025"
     CDS             <1..720
                     /locus_tag="VIF08_03025"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEY8134987.1"
                     /translation="PLRLDDMLLILAGIVPGAVVVGRALHGLDFWSYYASQPGKLFDP
                     SVGALSLLGAVIGGLMTGAYVARMIDAPVRRWADAAAVPMLLALGLGKLAQLLGGSGQ
                     GLPFDGVWAVAFTGAGPWVSTNPSLPSHPAQVYEGLWLLAGIPMVLLLAGQRYRPLRV
                     HRFVAWADRSARAGVVFAGALGWFLLGRLFIGYTWRDEAIVGGLNVEQTLALIAMLIL
                     LAYGVVHFRAERVVVPKASPR"
     gene            840..2381
                     /locus_tag="VIF08_03030"
     CDS             840..2381
                     /locus_tag="VIF08_03030"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY8134988.1"
                     /translation="MTQTADRPIETAPADAELGRVSHWIGGRIVEGTSGRHGPVFDPA
                     TGRQAKWVDFASVDEVDAAVAAAANAFPAWRATSLSKRTEIMFKIRNLVDQRRKEIAA
                     LLTAEHGKVPSDALGEVARGLENLEFATGIPHLLKGGFSEQVSGGIDVYQIRQPLGVA
                     AGITPFNFPAMVPMWMFGNAIACGNTFVLKPSEKDPSASLLMAELLHEAGVPDGVFNV
                     VQGDKVAVDRILNHPDVKAVSFVGSTPIAKYIYETGTRNGKRVQALGGAKNHMVVLPD
                     ADIDMAADAAVSAAYGSSGERCMAVSVVVAVGDVGDPLVKAIKERLPKINVGPGSDPA
                     AEMGPLVTREHRDKVASYLDKAGQQGATVVTDGREAEAFKSDGFFLGVSLIDNVQPDH
                     DSYKNEIFGPVLEVMRVKTYEDAVRLVNDNQYGNGTAIFTRDGGAARQFQFEAQAGMV
                     GINVPIPVPVAYYSFGGWKDSLFGDLHMYGPEGVQFYTRGKVVTARWPDPGTSKVDLG
                     FPRTR"
     gene            2392..2841
                     /locus_tag="VIF08_03035"
     CDS             2392..2841
                     /locus_tag="VIF08_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8134989.1"
                     /translation="MSGRFLEDFTPGQVIASTEWYDITPEGIHAFASEYDPQPIHLDP
                     AAAGESMFGGIIASGWHSLSVTMRLIVRADLFGGAPVVGVGVDNLRYLQPVRAGDRLT
                     AQAEVLGVKPSSSHPERGYLTLRVTTKRQDGMQVLTQDWTVLVPRRA"
     gene            2887..3117
                     /locus_tag="VIF08_03040"
     CDS             2887..3117
                     /locus_tag="VIF08_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134990.1"
                     /translation="MEGLGTAILIVAVGGILLLVVLAIEVAVLIRRLRQPDLCPDQRG
                     WLLMGGSLLVATAAMVVRRPLLKLDHCARRAS"
     gene            3166..4920
                     /locus_tag="VIF08_03045"
     CDS             3166..4920
                     /locus_tag="VIF08_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134991.1"
                     /translation="MKRALLAVLVALALAGCGSATPAPTQTVAPTHGLPSSQPTSGGP
                     SAGPSEQPTASSGTGPTDAPATPGPTGEDPLLEPVLTDAAAVADALFDPEQVAQGVVS
                     LLALMGVAIRDSAGTVVRAGSDRGAGELVLTDSQVRGLITMTRDDLTELGLHGGPYPL
                     ADMFTALQPSLPAGFTVEQLAAAYADAYSAEPEALAARVMRGQAIDAESGLLRIQEWV
                     LLIDGFLSPPQTASMTGSGTGRHGLARLAAGAALGTARQGLPALTSPSATLTNAEWAE
                     VIAALPTLASAIEFKVVASPGAIHEGHGAKGSPADFVAGIGVAAPLVSRSSGRTLLAA
                     APQPGIRVTWRSVDRTTWAKHGDFDPALGTPQLVDPGQPSGADLRYTPIKEDANGAGT
                     KLKEHPTLAARALQADLISHSYDTSSLGAALYLAAGARVATGPSSFEVGWHELGDGYV
                     IDIVWTDYYNGIADVITFSGIASRRDSGVQGIDAYYVGGGAVFGSRPGWTSCNPGVDV
                     VPSGFGQADFQAGIQNGEVVIAAYPQIGMPLSGIFAGPFILPAEGGSTTVSVPAQPGA
                     LCPHRVYGRIEAQPFGNP"
     gene            complement(4950..5249)
                     /locus_tag="VIF08_03050"
     CDS             complement(4950..5249)
                     /locus_tag="VIF08_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134992.1"
                     /translation="MTRWQYAWLTDQPQTTLGFSHPQPGLGGELTALLGPIVLTAQST
                     EWSIAFDRQRANLGYISGLMGDRGWEMFAVVNHPQGGPMGGVMQTNWYFRRPVEG"
     gene            5439..6488
                     /locus_tag="VIF08_03055"
     CDS             5439..6488
                     /locus_tag="VIF08_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134993.1"
                     /translation="MKVPARPSPKPSLLGVGALFLAALGVGAVVWVLLGGLPAAAPSP
                     STLATPTARVTRDPLEPLTIPSIQRGPHLIFQNVIRGDDYAKTSLVPLDSPGGMRLTT
                     GLICERVHFAAGHGICLTAEHGAESHYFAALFGADFVPTSRVPLDGAPTFARVSPDGR
                     MAAASFQTSPPEEEAPFAPSETWLLDTQSGQVVADLADFQLLRDSVQVTEPEVDYWGV
                     TFKRDSNGFYASVRFGGNIYLVEGNIAAKRLVVLNGGLSAPSLSPDGTRVAYASLVSS
                     IGPTWRFHVLDLTSMVDTALSETRSIDDQMEWLDNDELLYGLATDVWRVAADGSGQPS
                     PFLFGGLSPAVVEPL"
     gene            6498..>7388
                     /locus_tag="VIF08_03060"
     CDS             6498..>7388
                     /locus_tag="VIF08_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134994.1"
                     /translation="MSRAPVHRKPVVRWLISLALAALVMLLCVASAVAHPLGNTSVNL
                     YERVEIGAQEISVRFILDVSEFPALRERAFADTNDDGSVDEAESTAYLNSFWAYLEPF
                     LVLTADGQPLQMHRVRQTLTFPEGQGGLVLLRAVIELRAPQPSLAQGAVVAGALTETA
                     FQGVPGWHEIIVVGGSGVTLVDSNVPTTDLTSELTSYPPEMLTAPLSVRDAAFEYRLD
                     QPSATSAPTFQASAAPSPQPTGVDVGPRPTDPMVALLGNRVDLSSTLIGLLLATVLGA
                     VHALTPGHGKTLVAAYLVGTR"
BASE COUNT         1151 a         2493 c         2448 g         1296 t
ORIGIN      
        1 ccgttgcgcc tcgacgacat gctcctgatc ctcgccggca tcgtgcccgg cgcggtcgtg
       61 gtgggccgcg ccctccacgg gctcgacttc tggtcgtact acgcgagcca gcccgggaag
      121 ctgttcgatc ccagcgtcgg cgcgctgtcg ctgctgggcg ccgtgatcgg cggcttgatg
      181 acgggcgcct acgtggcgcg gatgatcgat gcgccagtgc gccgctgggc cgacgcggcg
      241 gccgtgccca tgctcctggc gctgggcctg ggcaagctcg cacagctgct tggcggcagc
      301 ggccagggcc tgccctttga cggcgtctgg gccgtggctt tcaccggcgc cgggccgtgg
      361 gtcagcacga acccatccct gccgtcgcac ccggcgcagg tctacgaggg cctgtggctg
      421 ctcgccggca tcccgatggt gctgctccta gccggccagc gctacaggcc gttgcgggtg
      481 catcggttcg tcgcctgggc cgatcgcagt gcgcgcgcgg gtgtcgtgtt cgccggcgcc
      541 ttgggctggt tcctgctggg ccgtctgttc atcggctaca cctggcgcga cgaggcgatc
      601 gtcggcggcc tgaacgtcga gcagacgctg gcgctcatcg cgatgctcat cctgctcgcc
      661 tatggcgtcg tccacttccg cgccgagcgc gtggtcgtac ccaaggcgtc gccgcgctga
      721 gccccgcccg acggggtatc gtgaccaagg acgacaatga cagccgctat tgcggcggtc
      781 agtggaggac gcgcagcagc agcccgcagg cgttgctgcg gagataggag caatttgacg
      841 tgacccagac cgccgaccgc cccatcgaga ctgcccccgc cgacgccgag ctcggccgcg
      901 tatcgcattg gatcggcggc cgcatcgtcg agggcacctc aggccgccac ggtccggtat
      961 tcgacccggc caccggtcgt caggcgaaat gggtcgattt cgcgtccgtc gacgaggttg
     1021 acgccgccgt ggcggccgca gccaacgcgt ttcccgcgtg gcgcgctacc tctctctcga
     1081 agcggaccga gatcatgttc aagatccgca acctggtcga tcagcggcgc aaggagatcg
     1141 ctgcgctcct gacggcggag cacggcaagg ttccctctga cgcgctgggc gaggtggcac
     1201 gcggcctgga gaaccttgag ttcgcgaccg gcatcccgca cctgctcaag ggcgggttct
     1261 cggagcaggt ctcaggcggc atcgacgttt atcaaattcg tcagcccctc ggcgtcgcgg
     1321 ccggcatcac gccgttcaac ttcccggcga tggtgcccat gtggatgttc ggcaacgcga
     1381 tcgcatgcgg caacacgttc gtcctgaagc cgtcggagaa ggaccccagc gcgtcgctct
     1441 taatggccga gctgctgcac gaggcaggcg tccccgatgg cgtgttcaac gtcgtccagg
     1501 gcgacaaggt ggcggtcgat cggatcctca accaccccga cgtcaaggcc gtctcattcg
     1561 tcggctcgac gccgatcgcc aagtacatct acgagaccgg caccaggaac ggcaagcgcg
     1621 tccaggcgct gggtggcgcc aagaaccaca tggtcgtact gcccgacgct gacatagaca
     1681 tggccgcgga cgccgcggtg tcggccgcgt atggcagcag cggcgagcgc tgcatggccg
     1741 tgtccgtggt ggtcgcggtg ggtgacgtcg gtgatccgct tgtcaaggcg atcaaggagc
     1801 gcctgcccaa gatcaacgtc ggaccgggca gcgatccggc ggctgagatg gggccgctcg
     1861 tcacgcgcga gcaccgcgac aaggtcgcct cgtatctcga caaggcgggc cagcagggcg
     1921 cgacggtcgt gaccgatggt cgcgaggcgg aggccttcaa gtcggacggc ttcttcctgg
     1981 gcgtgtcgct gatcgacaac gtccagccgg atcacgactc gtacaagaac gagatcttcg
     2041 gcccggtgct cgaggtgatg cgcgtcaaga cctacgagga cgcggtccgg ctcgtcaacg
     2101 acaaccagta cggcaacggc acggccatct tcacgcgcga cggcggcgcc gcgcgtcagt
     2161 tccagttcga ggcgcaggcg ggcatggtcg gcatcaacgt gcccatcccg gtgccagtcg
     2221 cgtactacag cttcggcggc tggaaggact cactcttcgg cgacctgcac atgtacgggc
     2281 cggagggcgt ccagttctac acgcgcggca aggttgtcac cgcgcgctgg cccgaccccg
     2341 gcacgtccaa ggtcgacctg ggcttcccgc gcactcgcta gcgttgcggc catgagcggt
     2401 cgcttcctcg aggacttcac gcccggccaa gtcatcgcct cgaccgagtg gtacgacatc
     2461 acacccgagg gcattcacgc cttcgcgtcc gaatacgacc cgcagccgat ccacctcgat
     2521 ccggccgcgg cgggggagag catgttcggc ggcatcatcg ccagcggctg gcactcgctc
     2581 agcgtgacca tgcgcctgat cgttcgcgcc gacctgtttg gtggcgcgcc cgtggtcgga
     2641 gtcggcgtcg acaacctgcg ttacctgcag ccggtgcgcg ccggtgaccg actgaccgcc
     2701 caggccgagg tcctcggcgt caagccgtcc tcgagccatc ccgagcgcgg ctacctgacg
     2761 ctgcgggtca cgaccaagcg ccaggatggc atgcaggtgc tcacccagga ctggacggtg
     2821 ctggtcccgc gccgcgcctg aatccgacac ttccgctcgg cgggcggctg gtgtataccg
     2881 gccggcgtgg agggcttggg aaccgcgatt ctgatcgtgg ccgtcggcgg gatactcctg
     2941 ctcgttgtct tggccatcga agtggcggtg ctgatccgcc gtctgcgaca gccggatctg
     3001 tgtcccgacc agcgcggctg gcttctcatg ggcggcagcc tgctcgtggc gacggctgca
     3061 atggtcgtgc gacgccccct cctgaagctc gatcattgtg cgcggcgcgc cagctgaagc
     3121 gctgaccatg cgccggccgt agtcacggat gtacggttgc ccacggtgaa gcgcgccctc
     3181 ctggcggtcc tcgttgctct cgccctcgct ggctgcggtt cggcgacccc cgcaccgact
     3241 caaacggtgg cgccgacaca cggcctgccg tccagccagc ccaccagcgg tggaccaagc
     3301 gccggtccat ccgagcaacc cacggccagc agtggcactg ggccgaccga tgcccccgcc
     3361 acccccggac cgacgggcga ggatccactg ctcgagccgg tgttgaccga tgccgcagca
     3421 gtcgcggacg ccctgttcga tcccgaacag gttgcacagg gcgtcgtttc gctcctcgcc
     3481 ctgatgggcg ttgccatccg tgactcggcg ggcacggttg tgcgcgccgg cagcgatagg
     3541 ggcgcgggcg agctcgtcct taccgactcc caggtgcgcg gcctcatcac catgaccaga
     3601 gacgacctga ccgagcttgg cctgcacggc ggtccgtatc cgctggccga catgttcacg
     3661 gcgctccagc ccagcctgcc cgcgggcttt acggtcgaac agctcgcggc tgcctacgcc
     3721 gatgcctatt cggctgaacc ggaagcattg gcggcgcgcg tcatgcgcgg ccaggcaatc
     3781 gacgccgaat cgggcctcct gcgcatccag gagtgggtgt tgctgatcga tgggttcttg
     3841 agcccgccgc agaccgcaag catgaccggg tcgggcacag gccggcatgg cctggcgcgc
     3901 ctggccgctg gcgccgcgct cggcacagcg cgtcagggcc tgcccgcgct gacctccccc
     3961 agcgcaaccc tcaccaatgc cgagtgggcc gaagtgattg ctgccttacc gacgctggcg
     4021 tcggccatcg agttcaaggt agttgccagt ccaggggcca tccatgaggg ccacggggcg
     4081 aaggggagcc cggcggactt tgttgccgga atcggcgtag ccgcgccgct ggtgtcgcgc
     4141 tcgagcgggc gcacgctgct tgcggctgcg ccacagccgg ggattcgcgt cacgtggaga
     4201 tcagtcgacc ggacaacctg ggccaaacac ggtgacttcg atcctgcgct cggcacgcct
     4261 cagctcgtcg atccggggca gccttcgggg gccgacttga gatacacacc catcaaggaa
     4321 gatgccaatg gcgccggcac caagctgaag gagcatccga cgctggctgc gcgagcactg
     4381 caggcagact tgatcagcca ctcgtatgac acgagctcgc tcggcgccgc cttgtacctc
     4441 gccgcgggtg cgcgcgtcgc caccggtccg agctcattcg aggtcggctg gcatgaactg
     4501 ggtgacggtt acgtgatcga catcgtctgg accgactact acaacggcat agccgacgtc
     4561 atcactttca gtggcattgc cagccggcgc gacagcggcg tccaggggat cgatgcctac
     4621 tacgtcggag gtggcgcagt attcgggtcg cgaccgggct ggacgtcgtg caatccgggg
     4681 gtcgacgtcg tgcccagcgg tttcggccag gcagatttcc aggcgggcat ccagaacggc
     4741 gaggtcgtga tcgccgcata cccgcagatc ggcatgccgc tgtctggcat cttcgccgga
     4801 ccgttcatcc tccccgcgga agggggatcc accacggtct cagtcccggc ccagcccggc
     4861 gcactctgtc ctcaccgcgt ctacggacga atcgaggcgc agccattcgg gaatccgtag
     4921 cacccgctga agagccgcgg cgccgcgcgt cagccctcga cggggcggcg gaaataccag
     4981 ttggtctgca tgaccccgcc catcgggccg ccctgcggat ggttgacgac ggcgaacatc
     5041 tcccagccgc ggtcgcccat caggccgctg atatagccca ggttggcgcg ctgccgatcg
     5101 aacgcgatgg accactcggt cgactgcgcg gtcagcacga tcgggcccag gagcgcggtc
     5161 agctcaccac ccaggccggg ctggggatgg ctgaagccga gggtcgtctg cggttgatcg
     5221 gtcagccagg cgtattgcca gcgcgtcatg tcaaagcctc ctactgcgca atccggcgct
     5281 tctactcggc cctcaggtac cattgatcgc tcgccagcga ccgtcggcgg ggtcaatgtc
     5341 gacgacgccc gctctcgatc ttcaactctg caacacctcg gtgatgttac ctatgggccg
     5401 cgccagatga ttcgtacagc ccgctgtttc gtcgcccatt gaaggtgcct gctcgtccca
     5461 gtccgaaacc gtcgctgctg ggcgtgggcg ccctgtttct ggcggccctc ggcgttggcg
     5521 ccgtcgtttg ggtgctgctc ggcgggctgc ccgccgcggc tccgtcacct tcgaccttgg
     5581 ccacgccgac agcgcgcgtg acacgcgacc cgctcgagcc actcactatc ccgtcgatcc
     5641 agcgcggccc ccacctgatc tttcagaacg tgatccgcgg cgacgactac gccaagactt
     5701 cgctcgtgcc actcgacagc ccggggggca tgcgtctcac cactggtctg atttgcgagc
     5761 gagtgcactt cgccgccgga cacggcatct gcctgaccgc ggagcacggc gcggagagcc
     5821 actatttcgc ggccctgttc ggcgctgact tcgtgccgac gagccgggtg ccgcttgatg
     5881 gcgcaccaac attcgcgcgc gtgtcgccgg atggtcggat ggccgccgcc tcattccaaa
     5941 ccagcccacc ggaggaggaa gctccattcg caccatcgga gacctggctg ctcgataccc
     6001 agagcggcca agtggtagcg gatctcgctg acttccagct gttgcgcgac agcgtccagg
     6061 tcacggagcc agaggtcgac tactggggcg tcacattcaa gcgagacagc aacggcttct
     6121 acgcctcggt gcgttttggc ggcaacatct acttggtaga gggcaacatc gcggccaagc
     6181 gcctggtcgt tctcaacggc ggactctcgg cgccgtcgtt gtcaccagac ggcacgcgcg
     6241 tggcatacgc cagtttggtt tcgagcattg gaccgacgtg gcgttttcat gtcctcgact
     6301 tgacgtcgat ggttgacacc gcactatcag agacgagaag catcgatgat cagatggagt
     6361 ggctggacaa cgacgaattg ctgtatggcc tggcgaccga cgtgtggcgt gtcgcggccg
     6421 acggctcggg ccagccctcg ccgttcctat tcggcggttt gtcgccagca gtggttgagc
     6481 cactctagtt gaacgcgatg tctcgagccc cggtacaccg caagccggtc gttcggtggc
     6541 tgatcagtct ggcgctcgcg gccttggtca tgttgctgtg cgtcgcatcc gcggttgcgc
     6601 atccgctggg caacacgtcg gtcaacctat atgaacgtgt tgagattggt gcccaggaaa
     6661 tttctgtcag gttcatcctg gacgtgtcgg aattcccggc gttgcgtgaa cgcgcattcg
     6721 ctgacactaa cgatgacggc tcagtggatg aagcagaatc gacggcatat ctgaatagct
     6781 tctgggcgta ccttgaaccc ttcctggtgc tgaccgcgga tggacaaccg ctgcagatgc
     6841 atcgcgtaag acagacgttg acgttcccgg aggggcaggg gggcttggtg ctcctgcgcg
     6901 cggtgatcga acttcgggct ccgcaaccgt cgctggcgca gggcgcggtt gttgcggggg
     6961 ccttgacaga gaccgcattc cagggcgttc cgggatggca cgagatcatc gtcgtcggcg
     7021 gctcgggggt gacgctggtc gacagcaatg tgccgacgac cgacctgaca agtgagctca
     7081 cctcataccc gccggagatg ctcactgctc ctctgtctgt tcgggacgcg gcattcgaat
     7141 accggctcga tcagccatcc gcgacatcgg caccaacctt ccaggcatct gccgctccgt
     7201 cgccacagcc gacaggcgtg gatgtcgggc ctcggcccac cgatcccatg gtggcgttgc
     7261 ttggcaatcg cgtggatctc tcatcgacgc tcattggctt gctgctggcg acggtgttgg
     7321 gcgcggttca tgccctgacg cccggccatg gcaagacgct cgttgctgcc tatctcgtcg
     7381 gaacccgg
//
LOCUS       DATBRX010000083        15644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2595314, whole genome shotgun sequence.
ACCESSION   DATBRX010000083 DATBRX010000000
VERSION     DATBRX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15644
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595314"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..492
                     /locus_tag="VIF08_03065"
     CDS             157..492
                     /locus_tag="VIF08_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8134995.1"
                     /translation="MVVDETGTPVEESGEAQRGGPTAAQFRRFIKSRPYVPLHELRRR
                     FELAGDLDDVSPVEMAGHGRVYLGLPPREAEFIATLVKDGDVGVELSRDPNVPIVVGV
                     YPMRPVTRS"
     gene            742..3087
                     /locus_tag="VIF08_03070"
     CDS             742..3087
                     /locus_tag="VIF08_03070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8134996.1"
                     /translation="MELTTASEPGKTPDYPQIELTLPIEGMTCASCVNRVERFLRKTD
                     GVLEANVNLATEQARVRFDPTVVGRDELHRAVVAAGYAVRTDAPAVEAGGALDAAAEA
                     DAQRRQRETRQLGWEAAAAIATGLVMMALTLWPTRPMPLEQLNVLLLVPATLVQFGLG
                     RRFYVSALRAARHASANMSTLVVLGTTAAWVYSAVITVAPGLVAGAGLEPMTYFDSAA
                     VIIGLVLAGRWLEARAKSATIGAVRRLAGLQARVARVVRDGQEVDVALAEVQAGDLVR
                     VRPGEKVPVDGRIVEGASALDESMLTGEPMPVAKGVGDEVIGATLNTSGSFVFRATRV
                     GRDTVLAQIVRLVEQAQGSKAPIQRLADAVTGWFVPAVLALAVATFVVWLIFGPEPRA
                     TFALVNLISVLVIACPCAMGLATPTAIMVGTGRGAEQGVLIRGGEALEAAERIDTVVF
                     DKTGTLTRGRPTLTEVVPAHGVDAAELLRVAAAVETGSEHPLAAAILEGAATRGVSAP
                     GATGFEAAAGYGAHAHIDGRLAIVGNARLMADHQVDLAPLHEAATRAATAAQTPVFVA
                     VDGRLLGLLAISDPVRPEAAAAVAELQRRGISTWLVTGDNRLVGESVARAVGIPVEQV
                     VADARPQDKIEFIKRIQARSRTAMVGDGINDAPALAQADLGVAIGTGTDVAMEASDVT
                     LVGGDLRLVATAISLSRATMRTIRQNLFWAFAYNVILIPVAMGVLYPFIGLLLDPILA
                     AAAMALSSVSVVANSLRLRRFRPPVRDSGDKGQIAPLPVAH"
     gene            3211..5451
                     /locus_tag="VIF08_03075"
     CDS             3211..5451
                     /locus_tag="VIF08_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY8134997.1"
                     /translation="MSKLARMLIAVALVVAAVPALLAGRGVSPVQAATTLLFADPFDG
                     NANPAWTVYVKAGGSVTWQSKTSVAAGALKAQSLANGRSLAYAQRALQGHPNVIAIDL
                     DLAASQSSATAQLPLVAVMGSSGRLFQLLRARDGQLVLQRSGKTLRLAGKLPLGAWRH
                     LTIDARGGSNPELTIGLAGKTIYDNSTPRFLNGASFYAMRLGSDREGRGGTSFFDSLV
                     VNAGGGTAPSPSPTRSPSPTSSPTATPSPTVSPSPSPTSSATPTFSPSPSPTSTSSPA
                     TCLLPPSPSPTPSTSPSPSVPPSVSPTPSSSPSAPPSSTPSPSASPSPSSSPTPTPTP
                     EPRFYFWGRGTDHGVGMSQWGARGRALAGQTYDEILHFYYTNVEIATIDGARPIRVRI
                     GSDFTPAAGCPARLTSVQGDWTSSAFDSQVFSVGSYLEMWPDAIAPAPPAPPAAPTPT
                     PAGWTAIVYSSQGTEIARTPTRDASMDSVLEANGLVKVSFKDSHPNMNQFRDSVRLMV
                     NSNGTLDQINTLPLDYYLLGVVPSEMPGSWHLEAVKAQAVAARSYAWPRIDGSGDYDI
                     GASSASQNYKGFLHEYPNSNRAVLETAGQVITYNGRVVAAYYHSSSGGHTENSEYAFV
                     TPAGNPGSVVGYCRGKPDVDANGLAYDRNSSDYYWESVSFTMTELSAIYATSSKTNVG
                     QILSLTFNRGVSGRAYQVVMTGTLGTKTVSGGAFSGIFNQNRPGGSNLVSNMFYLTPV
                     GEGPGG"
     gene            5566..7941
                     /locus_tag="VIF08_03080"
     CDS             5566..7941
                     /locus_tag="VIF08_03080"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY8134998.1"
                     /translation="MGNRAFHLRASLALVACLVALAVCVPAFVAAAPASSLEPVADAY
                     VSGGRATRNYGSVAILKARAGVLGAYLRFKLQPWVGQPADGLDLRLGGVVGDPTTLAL
                     MEVGGGWRETDLTFLTRPAPVSDIVVRGTVAEDGVHFPLGAFFDSGTVDRNYISLRVT
                     NDVATKVSFWSREGLAPPIVTLGSPPAGALLAARADTYTTPAAPDANYGAAARLAVDG
                     DPQAEAYLTFDTSAWLGRRIDAATLRLSLRDATGGALTIYRVDPGWDEQTLTWNTAAL
                     AGPAVATMAEAHPSGVAAIDISAAFADRVVDSPRLALRLVTTNADGFLFPARESGSSA
                     TLEMTPRSSTINPPPTATPSPTRTPSPTPTLTPSPTPTRSPSPSPTPTATPTPTATPS
                     PTPTPTPLFHFSGHGSDHGVGMSQYGARGRAAAGQTYDVILAHYYTGTSLGWVDPSQP
                     IRVMLNSAHVPTPDSPARIVAQIGPWTSAAFVDELGARTVFPADSYVELMPTTSGWQA
                     IASDSTGLVLATASTSDLTIDPADPATRLQMTWRSSLMRYALYRGSMRMLVSGTAVQA
                     INILPLDEYLQGVVPAEMPALWAIEAVKAQAVAARSYAMRHLHPERVFDVVPTADNQV
                     YGGVALEHPRSNMAVAQTGGQVVLYNGKVANTYFFAVGGGYTENNEYAWPSASGKAYD
                     DPVPYLRGVPDLDENGLPYDRNAPGFAWQSGSFTWAQLSAMMAADSRTNVGALRDIQF
                     KRGVSGRAYCVTLVGSARTVNVSGLVFKAVFNDHNGGGANLQSAMYFLEAE"
     gene            7982..8473
                     /locus_tag="VIF08_03085"
     CDS             7982..8473
                     /locus_tag="VIF08_03085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8134999.1"
                     /translation="MSRRPEPISGEFRYEHPVEVRFVDTDAFGHVNNATFLSYFEAAR
                     AGYYARVTGTPFMTGTHGLKHTFVIAEARIAYRSPVLFGEPLRAGVRFAWVGGSSFAF
                     EYRIRAEKSAVGEARVVADGETLQVMFDLDRNRVVRVPADLIGQFERFEERAIPQRPA
                     AKD"
     gene            complement(8470..9738)
                     /gene="fabF"
                     /locus_tag="VIF08_03090"
     CDS             complement(8470..9738)
                     /gene="fabF"
                     /locus_tag="VIF08_03090"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8135000.1"
                     /translation="MPEKDLARRAVVTGLGAVTPIGNDHPTFWKNLVAGVSGAGPITR
                     FDASNFDVRIAAEVKGFDPTVAMDRKMARRMSRFIHFAMAVAKEAVADARIDFSEWSP
                     EQRDRAAVIINSAGGGVEEILMNDGVLREKGPGQVSPFAYPALSASMAGCLVSMEYGI
                     TGPVINQSAACATSIVAFLDGLRLIQSGEMDVVFAGGSEAPLLQVGFAALGNMHALSR
                     RNDDPTRASRPFDRDRDGFVYGEGAGVVMLESAAHALARGATIHAELVGAAMTSDAFH
                     ISAPDPTGRGATMAMTRALQDAAVAPDEVDYIAAHGTSTALNDATETRAIKAAFGAHA
                     HKVAISSPKSMVGHTIGAAGVVNALAAIGAIREGVIAPTVNLDNPDLPELDLDYVPLV
                     ARQAKVDIAMINGFGFGGQNAVAVFRRFEP"
     gene            complement(9749..10609)
                     /locus_tag="VIF08_03095"
     CDS             complement(9749..10609)
                     /locus_tag="VIF08_03095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEY8135001.1"
                     /translation="MTDARQPDPVLGFYGPDSMMWRINREAVLLGAGPAALLLQVAHP
                     LIAEGVAHHSSFEQDPWRRLHGTIRTTMDLVFGDGVVANRAVRRLNSVHAGVRGDVLD
                     DEARRVARAYRALDPALLLWVQATLIVTSVAAYQRWVGPLSAEEREQFWQEARAVGVR
                     LGIPLSLSPADWPSLEAYWAKMLADDGPVHVTPTARRLARLIVRPPFPFVPGPLVDVA
                     ALPGLALLPPRIRSEFDITWGAPKAALAETIGTAVRLWTRIVPRRLRSMPQALAADRR
                     ARRATISGHP"
     gene            10620..11984
                     /locus_tag="VIF08_03100"
     CDS             10620..11984
                     /locus_tag="VIF08_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEY8135002.1"
                     /translation="MALDLLIRNVTLVSPAGQRVVNIFISDGQFSAILDPGPLPFAPA
                     AETIDGTGLVALPGLIDGHVHFREPGLTHEETWLSGTRAAVFGGVTTVLDMPNTVPPT
                     DSVERAREKLALADLTAYCDFGIFGLLGQDTVQTGDLAGSGLAVGLKAFVGPTTGNLP
                     APDEDELFRGLEQARVAGLRVAFHAEDRATIDALRAHETRTDPIAHLETRPAEAEVRA
                     IERIGALLEATRARGHICHVSSSAGLDAVRDWQQRDVDVTCEVTPHHALLGREVYADF
                     GGVAKVNPPIRGEADASALLAALADGRINTVASDHAPHLAADKVRASIWDVPSGFAGV
                     ETLLSLMLTAVHDGRLSLERLVHATSEAPAKTWGLWPHKGQVAVGADADLTLVDVERA
                     GEIHAADLHGMNNISPFEGRATLGAVVATLVRGKVVVRDGQLVGEPGWGQPVRTNSRS
                     SSTA"
     gene            complement(11942..13090)
                     /locus_tag="VIF08_03105"
     CDS             complement(11942..13090)
                     /locus_tag="VIF08_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8135003.1"
                     /translation="MTTKLDAAAARRIAVAAALLGARPLPPATDSVVEVARHFGGIQI
                     DPTRTVERTQHLVLWSRISGYERTLLDRVLAERRAFEFDAFIVPVERLPELRHVARTW
                     LDGPGSWRAKTRRFLDGNVAFRRSIVDQLRANGPMQSRQFDDSKVLVGWESSGWTHGK
                     NTTRMLEFMGVMLEVVVAGRIGQERLWDLPERVFPRDAPHTQLTRGQYDEARVMRAMR
                     RFGVATQQEIRLRAYGLSIPDAKAMFARLVEEGRLTAVRLALTGGEVDAFALPDALAA
                     ADNATRTTLVSPFDPLVYDRDRTARLFDFAYKLEMYKPVALREFGHFVLPIVHHEKLV
                     GRLDSERDRKKNELVVRKLHWEGKTPAAPVRAAVNQAVDELREFVRTG"
     gene            complement(13272..14543)
                     /locus_tag="VIF08_03110"
     CDS             complement(13272..14543)
                     /locus_tag="VIF08_03110"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8135004.1"
                     /translation="MGIRRPDFNRRVAVTGLGIISPIGQDKDAVWDSLVNAKTGLHRI
                     TRWDPTPYEAQISGEVNDFDGTKWMDFKAIRRTDRNVVLGVAASKMAVADSGLEVTDD
                     NRDDIGIYFGSGGGGPYLLMENLEKWKASGARTVSPFFIANMLPDTASGQIAIETGIR
                     GSNMCIVTACSTGTHNIGEAAEGIRRGDFVAAIAGATENPLHEMVYIGFSNMRGMGKP
                     RDGEPVETVSRPFDKTRDGFVLGEGSGALVLEDLEYAKARGATIYAEVVGYGSAADAW
                     DLIQPVEQGDGARRAMIQALERHGVPADQVDLINPHGTSTPLGDLREAQAIWATFGDH
                     TPNVAISATKSLTGHLMGAAGAVEGVFTVLSVYHQTAPATANYRDDDPEINLNVVHGE
                     AKKMEIRYAMSDNIGLGGHNGAVIFKRYEGD"
     gene            complement(14561..15508)
                     /gene="fabD"
                     /locus_tag="VIF08_03115"
     CDS             complement(14561..15508)
                     /gene="fabD"
                     /locus_tag="VIF08_03115"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8135005.1"
                     /translation="MAFAFVFPGQGSQYVGMGKPLSESSERVATTMAAVDMVLDEPLS
                     RLMFDGPAEQLDQTINSQPAILACSIAYLRAIEERLRSWEQELPPPAFYAGHSMGQYS
                     AMVAAGVISLADGVRLVRERGKQMQASAEDGSMAAVIGLPDEALSALESAGQQLGVFT
                     IANRNSPGQIVVSGERAAVEGAAEKAKELGAKRAIVLPVSVAAHSPLMSKAADAMREV
                     LATVAFNDPSAPLLANADARPLTTAEECRAELVEHLTRGVDWVKAVEAMSARGVGRFV
                     EVGPGKVLTNLIKRINSESEAVAVDEQSAPDRFFMPEFL"
BASE COUNT         2471 a         5429 c         5204 g         2540 t
ORIGIN      
        1 ggcccccctg gcggccggca acgtcttcag catcgagccg ggcatctaca tcgagggccg
       61 gtttggcgcg cgcatcgagg acctggtcgt gtgcggcgcg gatggtgtcg acgcgctcaa
      121 cgtctcgtcg cgtggcctgt acgtggtgcg cggctgatgg tggtcgacga gaccggcaca
      181 ccggtcgagg aaagcggcga ggcacagcgc ggcggtccga ctgccgccca gttccggcgc
      241 ttcatcaagt cgcgtcccta cgtgccactg cacgagctgc gccgacgctt cgagctggcc
      301 ggcgatcttg acgacgtctc accggtcgag atggcgggcc acggccgcgt gtacctgggg
      361 ctgccgccgc gcgaggcgga gttcatcgcg accctggtca aggacggcga cgtaggggtc
      421 gagctgtcgc gcgacccgaa cgtgccgatc gtcgtcggcg tgtacccgat gaggccggtc
      481 acccgcagct gaccctcccc ggaccgctcg cgtgagccac attcacgaat tccgtggctg
      541 gctcgccttc cgccgtgaat cggggagtat ccggaccttc tgaggcgcct aaccgccatc
      601 gccgagtcgc aggtgattgc ccatgccgca gcctccgcct ccaaacggct tcgtcggcag
      661 ccaagggtcg cactcaagct cgcatttcgt gaatgacgtt cacattcgta tcggcgaagc
      721 agccgtatac taggggggag tatggagctg accaccgcca gcgagcccgg caagaccccc
      781 gattacccgc agatcgagct cacgttgccg atcgagggca tgacctgcgc ctcatgtgtg
      841 aaccgggtcg aacgcttcct gcgcaagacg gacggtgtcc tcgaggccaa cgtcaacctg
      901 gcgaccgagc aggcacgggt gcgcttcgac cccacagtcg ttggtcgcga cgagctgcat
      961 cgggcggtgg tcgcggcggg ttacgcggtt cgcacggacg cgcccgcggt cgaggccggc
     1021 ggtgcgcttg atgccgcggc tgaagcagac gcccagcgcc ggcagcgcga gacgcgccag
     1081 ctgggctggg aggcggccgc ggccatcgcg acgggtctcg tgatgatggc gctgaccctg
     1141 tggcccacgc gacccatgcc attggagcag ctcaacgtgc tgctgctagt gccggcgacg
     1201 ctggtccagt tcggcctggg tcggcgcttc tacgtctcag cgctccgggc ggcacgccac
     1261 gcgtcggcca acatgagcac gctcgtcgtg ttgggcacga ccgcggcatg ggtctactcg
     1321 gccgtgatca cggttgcgcc cggtctcgtg gccggggctg gtctcgagcc gatgacctat
     1381 ttcgacagcg ccgcggtgat cattggcctc gtcctggccg ggcgctggct ggaggcgcgc
     1441 gcaaagtcgg ccacgatcgg tgccgtgcgc cgcctggccg gactccaggc acgcgtcgcc
     1501 cgggtggtgc gcgacggaca agaggtggac gtggcgctgg ccgaggtcca ggcgggtgac
     1561 ctcgttcgcg tccggccggg tgagaaggtg ccggtcgacg gccggatcgt ggaaggcgca
     1621 tcggcgctcg acgagtcaat gctgaccggc gaacccatgc ccgtcgcgaa aggcgtcggc
     1681 gacgaggtca ttggggcaac gctcaacacc agcggctcat tcgtattccg cgcgacgcgc
     1741 gtcggacgcg acacggtgct ggcgcagatc gtgcgcctgg tcgagcaggc gcagggctca
     1801 aaggcgccca tccagcgcct tgctgacgcg gtcaccggct ggttcgtgcc cgcggtgctc
     1861 gctctcgccg tcgcgacgtt tgtcgtgtgg ctcatatttg gcccggagcc acgggcgacg
     1921 ttcgcgctgg tcaacctgat cagcgtgctg gtcatcgcct gcccgtgcgc gatgggcctc
     1981 gcgacgccga ccgcgatcat ggtcgggacc ggccgcggcg cagagcaggg cgtgctgatt
     2041 cgcggcgggg aggcgctcga ggccgcggag cgcatcgaca cggtggtctt cgacaagacc
     2101 ggcacgctga cccgcggtcg ccctacgctg accgaagtcg tgccggccca cggcgtcgat
     2161 gccgccgagc tgctgcgggt ggcggcggcc gtggaaacgg gcagcgaaca cccacttgcc
     2221 gcggcaattc tcgagggagc ggccacacgt ggcgtcagcg cgccgggcgc gaccgggttc
     2281 gaagccgcgg ccggctacgg cgcccacgcc cacatcgacg gtcgcctcgc gatcgtgggc
     2341 aacgcccggc tcatggctga ccaccaggtc gacctcgcac cgctccacga ggccgcaacg
     2401 agggcggcca cggccgccca gaccccggtc tttgtcgccg tagatggacg gttgcttggc
     2461 ctcctggcga tctccgaccc tgttcgtccc gaagccgccg cagcagtcgc cgagctccag
     2521 cgccgtggca tcagcacctg gttggtgacc ggcgacaacc ggctggtcgg cgaatcggtc
     2581 gcccgcgccg ttgggatccc tgttgagcag gtcgtcgccg acgcccggcc ccaggacaag
     2641 atcgagttca tcaagcggat ccaggcaagg tcccggaccg cgatggtggg cgacggcatc
     2701 aacgatgcgc cggcgctcgc gcaggccgat cttggcgtcg ccatcggaac gggcaccgac
     2761 gtggccatgg aggcgtccga cgtgacgctc gtcggcggtg acctgcgcct ggtggcgacg
     2821 gcaatcagcc tgtcgcgcgc gaccatgcgc accatccgcc agaacctgtt ctgggcgttc
     2881 gcctacaacg tcatcctgat cccggtcgcg atgggcgtgc tctatccctt catcggcctg
     2941 ctgctggatc ccattcttgc cgccgccgcc atggcactga gctcggtcag cgttgtggca
     3001 aactcacttc gcctgcgccg tttcaggccg cccgtacggg attcgggcga caaaggtcag
     3061 atcgcgcccc tgccggtggc tcactagagt tagtgccagt cggccacggg ccgacctgcc
     3121 gtcacctcgg ggggccgaaa catagggctt cgtactccac cagtgtcaga tgacgctcgc
     3181 ccggcatcta ctggtcggga ggtaaacgca ttgtcgaagc tggcccgcat gctcatcgcc
     3241 gtggccctgg tggtcgcggc tgtgcccgcg ttgctcgccg ggcgcggcgt ctcccccgtc
     3301 caggcagcca ccacgctgct cttcgctgac cccttcgatg ggaatgcaaa tcccgcctgg
     3361 acggtatacg tcaaggccgg cggcagcgtc acatggcagt ccaagacgag cgtggctgcc
     3421 ggtgcactga aagcccagtc tctggccaat ggccgctcgc tcgcatatgc gcagcgggcc
     3481 ctgcagggac accccaacgt catcgcgatc gacctggacc tcgccgccag ccagtcgtcg
     3541 gcgacggctc agctgccatt ggtcgccgtg atgggcagca gcggccggct gttccagctg
     3601 ctccgcgccc gagacggcca gctggtcctg caacgctcgg gcaagacgct tcgcctggcg
     3661 ggcaagctgc ccttgggcgc atggcgccat ctcacgatcg atgcccgcgg cggctccaac
     3721 ccggagctca cgatcggcct cgcgggcaag acgatctatg acaacagcac accgcgcttc
     3781 ctgaacggcg cgagcttcta cgccatgcgc ctcggcagcg accgcgaggg tcgcggcggc
     3841 acgagcttct ttgacagcct ggtggtcaac gccggcggtg gcaccgcacc atcgccgtcg
     3901 cctacgcgat cgccgtcgcc tacgtcgtcg ccgacggcca cgccttctcc aaccgtttca
     3961 ccttcgcctt cacccacctc atcggcgacg ccgaccttct caccttcgcc ttcaccaacc
     4021 tcgacgtcat cgcctgcgac ttgccttctg ccgccgtcgc cgtcgccgac accttcgacg
     4081 tcgccatcgc cgtcggtgcc cccgtcggtg tcccccacac ccagctcgtc accgtcagcg
     4141 ccacccagca gcacaccgtc gccctccgcc agcccgtcgc cttcgtcctc gcccaccccc
     4201 acgccaacgc cggaaccgcg cttctacttc tggggccgcg gcaccgacca cggcgtgggc
     4261 atgtcccagt ggggcgcccg gggccgtgcc ctcgcggggc agacctacga cgagatcctc
     4321 cacttctact acaccaacgt cgagatcgcg acgattgatg gggcgcggcc catccgcgtc
     4381 cgcatcggca gcgacttcac tcctgcggcc ggctgcccgg cgcggctgac ttcggtgcag
     4441 ggcgactgga cctcgtcggc ctttgacagc caggtcttct ccgtcggcag ctacctcgag
     4501 atgtggccgg acgcaatcgc gccggcgccg cccgccccgc cggcagcgcc gacccccacg
     4561 ccagctggct ggacggccat cgtctacagc tcccagggca ctgagatcgc gcgcacgccg
     4621 actcgcgatg catcgatgga ttcggtcctg gaggccaacg gcctggtcaa ggtgtccttc
     4681 aaggacagcc acccgaacat gaaccaattc cgcgactcgg tcaggctgat ggtcaacagc
     4741 aacggcaccc tcgaccagat aaataccctg cccctcgatt actacctgct gggggtcgtg
     4801 ccatcggaga tgccaggcag ctggcacctc gaggccgtga aggcgcaggc agtagcggcg
     4861 cgtagctacg cctggccgcg gatcgacggc agcggcgact acgacatcgg ggcgtcgtcg
     4921 gcgtcccaga actacaaggg cttcctgcac gagtatccga acagcaatcg cgccgtcctc
     4981 gaaaccgccg gacaggtcat cacgtacaac ggccgcgtgg tcgccgcgta ctaccactca
     5041 tcgtcaggcg gccacacgga gaacagcgag tacgcattcg tgactccggc gggcaacccg
     5101 ggctccgtgg tcggctactg ccggggcaag ccggatgtcg acgccaatgg ccttgcctat
     5161 gatcgcaact cgagcgacta ctactgggaa agcgtctcgt tcaccatgac cgagctctcc
     5221 gcgatctacg ccacatcgag caagacgaat gtcggccaga tcctcagcct gacgttcaac
     5281 cgcggcgtat ccggccgcgc ctaccaggtg gtgatgacgg gcacgctcgg caccaagacg
     5341 gtgtccggcg gcgcgttcag tggcatcttc aaccagaacc gtcccggcgg ctcaaacctc
     5401 gtgagcaaca tgttctatct gacacccgtg ggcgaggggc cgggcggcta ggtcgcgctg
     5461 cctggccaac tgggcactcg tgccatccgc acctgacagt accgatctgg ggccggacca
     5521 acgtgacatg gtccgaaggg gcctcccgac ctagggtcgg gaggagtggg aaaccgtgct
     5581 tttcacctgc gtgcaagcct ggccctcgtt gcctgtctcg tggcactcgc cgtgtgcgtg
     5641 ccggccttcg tggccgcggc cccggcgagc agcctggagc ccgttgccga tgcctatgta
     5701 agcggcggtc gggcgacgcg caactacggc agtgtcgcga ttctcaaggc ccgcgcgggc
     5761 gtgctcggcg cctacctgcg cttcaagctc cagccgtggg tcggtcagcc ggcggatggc
     5821 ctcgacctgc gactgggcgg cgtggttggc gatcccacga ccctggcgct gatggaggta
     5881 ggcggcggct ggcgggaaac ggacctgaca ttcctgacgc ggcctgcgcc ggtgagcgac
     5941 atcgtcgtgc gcggcaccgt ggccgaagat ggcgtgcact ttccgctggg cgcgttcttc
     6001 gacagcggca ctgttgatcg caactacatc tcgctgcgcg tcaccaatga tgtcgcgacg
     6061 aaggtcagct tctggtcgcg cgagggcctg gcaccgccga tcgtgacgct gggcagccca
     6121 cctgccggcg cgctgctcgc cgcgagggcc gacacatata cgacgccggc cgcgccagac
     6181 gccaactatg gtgccgcggc ccggctggcg gtcgacggcg atccacaggc agaggcgtac
     6241 ctcacgtttg acacgtcagc ctggttgggc cggcgcatag acgcggcgac gctgcggctc
     6301 agcctgcgtg acgcgaccgg tggagcattg acgatctacc gcgtcgaccc cggttgggac
     6361 gagcagacgc tcacctggaa cacggccgcc ctggcagggc cggccgtcgc gacgatggcg
     6421 gaggcacatc ccagcggtgt ggccgcgatc gacatcagcg ccgcgttcgc ggaccgcgtc
     6481 gtcgatagcc cgcgcctcgc gctgcgcctg gtcaccacga acgctgacgg cttcctcttc
     6541 ccggcgcgcg aaagtggctc gtcggcaacc ctggagatga caccgcgctc aagcaccatc
     6601 aacccgccgc cgacggccac gcccagcccg acgcgcacac cttcgcccac cccgacgctc
     6661 acaccgtcac cgacccccac acgctccccg agcccaagcc cgacccccac cgccacgccc
     6721 acacccacgg ccacgcccag tcccactcca acccccacgc cgctcttcca cttcagcggc
     6781 cacggctcag atcacggcgt gggcatgtct cagtacggcg cacgcggccg cgcggcagcc
     6841 ggacagacat acgacgtgat cctggcgcac tactacaccg ggacgtcgct cggctgggtt
     6901 gacccaagtc agccgatccg ggtgatgctc aactcggccc acgtgcccac gccggacagt
     6961 ccggcgcgga tcgttgcgca gatcggtccg tggaccagcg cagcattcgt ggacgagctt
     7021 ggcgcgcgga ccgtctttcc ggccgattcc tacgtcgagc tgatgccgac cacgtcgggc
     7081 tggcaggcaa tcgcatccga ctcgaccggg ctggtcctgg ccaccgcctc cacgagtgat
     7141 ctgacgatcg acccggccga tccagccacc cgcctacaga tgacctggcg ctcgtctctg
     7201 atgcgttacg cgctgtaccg cggctcgatg cgcatgctgg tcagcggcac cgcggttcag
     7261 gcgatcaaca tcctgccgct cgatgaatat ctgcagggcg tcgtgcctgc ggagatgccc
     7321 gccctgtggg cgatcgaggc ggtcaaggcg caagcggtgg cggcacgcag ctacgccatg
     7381 cgccacctcc atcccgagcg cgtgttcgac gtagtgccaa ccgctgacaa ccaggtctac
     7441 ggcggcgtcg cgctggagca ccctcgctcc aacatggcag ttgcccagac cggtggccag
     7501 gtggtcctgt acaacggcaa ggtcgccaac acctacttct tcgcggtcgg cggcgggtac
     7561 acggagaaca acgagtacgc atggccgagc gcatccggca aggcctacga cgaccccgtc
     7621 ccgtacctgc gcggtgtgcc cgaccttgac gagaatggcc ttccgtacga tcgcaacgca
     7681 cccggcttcg cgtggcagag cggctcgttc acctgggcgc agctcagcgc gatgatggcc
     7741 gccgactcgc ggaccaatgt cggcgccttg cgggacattc agttcaagcg cggcgtgtcc
     7801 ggccgcgcct attgcgtgac gctcgtcggc agcgcgcgca cggtcaacgt ctccggcctg
     7861 gtcttcaagg ccgtgttcaa cgaccacaac ggcggtggcg ccaatctcca gagcgcgatg
     7921 tacttcctcg aggccgagta gcaccccatt cgttcgccgc gggccatagc attcagcgac
     7981 cgtgagccgc cggcctgaac caatcagcgg tgaattccgc tacgaacatc ccgtcgaggt
     8041 ccgcttcgtc gacaccgacg cgttcggcca tgtcaacaac gcgacttttc tgtcgtactt
     8101 cgaggcggcg cgagccggct actacgcgcg cgtgaccggc actcccttca tgaccggcac
     8161 gcacggcctg aagcacacct tcgtcatcgc cgaggcgcgc atcgcctacc gctcgcccgt
     8221 gctctttggc gagccgctgc gcgccggtgt ccgcttcgcc tgggtgggcg gttcgtcatt
     8281 cgcgtttgag taccgcatcc gggccgagaa gtcggccgtg ggcgaagccc gcgtggtcgc
     8341 ggacggcgag acattgcagg tgatgttcga tcttgaccgc aaccgcgtgg tgcgcgtgcc
     8401 ggccgacctg atcggccagt ttgaacggtt cgaggagcga gcgattccgc agcgaccggc
     8461 cgcgaaggat taaggctcga acctccggaa cacggcgacg gcgttctggc cgccaaaccc
     8521 gaagccgttg atcatcgcga tgtccacctt ggcctggcgc gcgacgagcg gcacgtagtc
     8581 gagatcgagc tcaggcagat cagggttgtc caggttgacc gtcggcgcga tgacaccctc
     8641 gcggatcgcg ccaatcgccg ccagggcgtt gaccacaccg gccgcgccga tggtgtggcc
     8701 gaccatcgac ttgggtgagc tgatcgcgac cttgtgagca tgcgccccga aagctgcctt
     8761 gatggcgcgc gtctccgtcg cgtcgttcaa cgccgtcgac gtcccgtggg cagcgatgta
     8821 atcaacctca tccggtgcca cggccgcatc ctgcagagcg cgggtcatgg ccatggttgc
     8881 gccgcggccg gttgggtcgg gcgccgagat gtggaacgcg tcggacgtca tggcggcgcc
     8941 cacgagctca gcgtgaatgg tcgcgccacg tgccagcgcg tgcgcggccg actcgagcat
     9001 tacgacgcct gcgccctcgc catagacgaa gccgtcgcga tcgcggtcga aggggcgcga
     9061 tgcgcgcgtg ggatcgtcgt tacgccgcga cagcgcatgc atgttgccca gggccgcaaa
     9121 gcccacctgg aggagcggcg cttccgaccc gccggcaaag acgacgtcca tctcgccgct
     9181 ctggatcagg cgcaggccgt caaggaacgc gacgatcgag gtagcgcacg ccgccgactg
     9241 gttgatgacc ggcccggtga tgccgtactc catcgagacc aggcagcccg ccattgacgc
     9301 cgacagcgcc ggataggcga acggactgac ctggcccggg cccttctcgc gcagcacccc
     9361 atcgttcatc aggatctcct cgacgccgcc gcccgccgag ttgatgatca ccgccgcgcg
     9421 atcgcgctgc tccggtgacc actccgagaa gtcgatcctc gcgtcggcga cggcctcctt
     9481 ggcgacggcc atcgcgaagt ggatgaagcg gctcatgcgc cgcgccatct tgcggtccat
     9541 ggccaccgtg ggatcaaagc ccttgacctc ggccgcgatg cgcacgtcga agttggaggc
     9601 gtcgaaacgc gtgatcgggc ccgcgccgct gacgccggcg acgaggttct tccagaaggt
     9661 ggggtgatcg ttgccgatcg gcgtgactgc gccaaggccg gtgactacgg cacggcgggc
     9721 gaggtccttt tcgggcacgt gagaactcct aggggtgtcc ggaaattgtc gcacggcggg
     9781 cccgccgatc ggccgccagc gcctgtggca tcgagcgcag ccggcgcggg acgatgcggg
     9841 tccataggcg caccgccgtg ccaatggttt cggccagcgc cgccttgggc gcgccccagg
     9901 tgatgtcgaa ctcgctccgg atgcgcggcg gaagcagcgc caggccgggc agggcggcga
     9961 catcgaccag cggcccgggc acgaacggga agggcgggcg cacgatcagc cgcgccaggc
    10021 gacgcgcggt gggagtcacg tggaccggcc cgtcgtcggc gagcatcttg gcccaatagg
    10081 cctcgagcga cggccagtcg gcagggctga gcgagagcgg gatgcccagc cgcacgccca
    10141 ccgcgcgtgc ttcctgccag aactgctcac gctcttcggc gctgagcggc cccacccagc
    10201 gctgatacgc cgcgacgctc gtcacgatca acgtcgcctg gacccacagc agcagcgctg
    10261 gatcgagggc acggtatgcc cgggcgacac gccgcgcctc gtcgtcgagc acgtcgccgc
    10321 gcaccccggc gtgaacggag ttgagacgcc tgactgcccg attcgcaact accccgtcgc
    10381 cgaacaccag gtccatcgtc gtccgaatcg tgccgtgcag ccggcgccac gggtcctgct
    10441 cgaagctgga gtggtgtgcg acaccttcgg cgatcagcgg atgcgctacc tgcaggagca
    10501 gcgcggctgg tccggcgccg agcagcaccg cctcgcggtt gatccgccac atcattgaat
    10561 cgggcccgta gaagccgagg actgggtccg gttggcgtgc gtcggtcaca cttggcatcg
    10621 tggccctaga tctgctgatc cgcaacgtga ccctcgtctc gcccgccggg cagcgcgtcg
    10681 tgaatatctt catcagcgat ggccagttct cggcgatcct cgatcccgga ccgctgccct
    10741 tcgctcccgc cgctgagacg atcgacggca ccggcttggt ggcgctgccc ggtctcatcg
    10801 acggtcacgt ccactttcgc gagccgggcc tgacgcacga ggagacgtgg ctgagcggca
    10861 cgcgcgccgc ggtgttcggc ggcgtgacga ctgtgctcga catgcccaac acagtgccgc
    10921 cgaccgattc tgttgagcgc gcgcgagaga agctggccct ggccgacctg actgcttatt
    10981 gcgacttcgg catcttcggg ctcttgggtc aagacactgt tcaaaccggc gaccttgccg
    11041 gatcgggact ggcggtcggc ttgaaagcgt ttgtggggcc gactaccggc aaccttcctg
    11101 ccccagacga agacgagctc tttcgcgggc ttgaacaagc tcgagtggcc ggattgcgcg
    11161 tggcgtttca cgccgaggac cgtgcgacta tcgacgctct ccgcgcgcac gagacgcgga
    11221 ccgatccgat cgcccatctc gagacgcggc cggcagaggc cgaggtcagg gcaatcgaac
    11281 ggatcggggc gctcctcgag gccacgaggg cgcggggcca catctgccac gtgtcgagct
    11341 cggccgggct ggacgcggtg cgagactggc aacagcgcga cgtggacgtg acctgcgagg
    11401 tgaccccgca tcatgcgctg ctggggcggg aggtctatgc cgacttcggc ggcgtggcca
    11461 aggtcaaccc gcccatccgc ggcgaggcgg acgcgtcggc cctgctggct gccctcgccg
    11521 acggccgcat caacacggtc gccagcgacc acgcgccgca cctcgccgcg gacaaggtgc
    11581 gcgcctccat ctgggacgtg ccgagtggct tcgccggcgt ggagaccctg ctgtcgctga
    11641 tgctgactgc cgtgcacgat ggccggctgt cgctcgagcg actcgttcac gcgacctctg
    11701 aggcgccggc gaagacctgg ggcctgtggc cacacaaggg ccaggtcgca gtcggggcag
    11761 atgctgacct gacgctcgtc gacgtggagc gtgccggcga gattcatgcc gcggatctgc
    11821 acggcatgaa caacatctcg ccgttcgagg gtcgcgcgac cctcggcgcg gtcgtcgcca
    11881 ccctcgtccg cggcaaggtc gtggttcgcg acggccagtt ggtcggcgaa cccggctggg
    11941 gtcaacctgt ccgcacgaac tcgcgcagct cgtctactgc ctgattgacc gcggcgcgca
    12001 ccggcgcagc aggggtcttg ccctcccagt gcagcttgcg caccacgagc tcgttcttct
    12061 tccgatcgcg ctcgctgtcc agccgcccga cgagcttctc gtggtggacg atgggcagta
    12121 cgaagtggcc gaactcacgc agcgcgacgg gcttgtacat ctcgagcttg taggcgaagt
    12181 cgaacagccg cgcggtgcga tcgcggtcgt agaccagcgg gtcgaatggt gagaccaggg
    12241 tcgtgcgtgt ggcgttgtcg gcggcagcca gggcatcggg cagggcaaac gcgtcgactt
    12301 cgccaccggt tagcgcaagg cgcaccgcgg tcaatcggcc ctcctcaacg agtcgagcga
    12361 acatcgcctt cgcatcaggg atcgacaggc cataggcgcg cagccggatc tcctgttgcg
    12421 tggcgacgcc gaagcgccgc atcgcgcgca tgacccgcgc ctcgtcatat tggccgcgcg
    12481 tcagctgtgt gtgtggggcg tcgcgcggga agacgcgctc gggcagatcc cacagccgtt
    12541 cctggccgat cctgcccgcg acgaccacct cgagcatgac gcccatgaac tcgagcatgc
    12601 gcgtcgtgtt cttgccgtgc gtccagccgc tcgactccca gccgaccaac accttcgagt
    12661 cgtcgaactg gcgcgactgc atgggcccgt tcgcgcgcag ctgatcgacg atggaccggc
    12721 ggaacgcaac attgccgtcg aggaacctgc gcgtcttggc gcgccatgag ccggggccgt
    12781 ccaaccatgt gcgcgccacg tggcgtagct ccgggaggcg ttctaccggc acgatgaagg
    12841 cgtcgaactc gaatgcacgc cgctcggcca ggacgcgatc gagcagggtt cgctcgtagc
    12901 cgctgatgcg gctccatagc acgaggtgct gcgtccgctc gacggtccgc gtcgggtcga
    12961 tctggatgcc cccgaaatgg cgcgccactt cgaccacgga atcggtcgcc ggcggcagag
    13021 ggcgtgcccc gaggagcgcg gccgcgaccg caatccgccg cgcggctgct gcgtcgagct
    13081 tcgtcgtcac gtgctttggc tcaattctcg gtagtggcga gacgggcacg gcctatcacg
    13141 gtcgcttggt gcgatttgtg gttctgcgcc agaaacgcaa cgacatgatg gcgcgctctc
    13201 gctatcggcg agaaacgggc caaagtgatg gctgaggacc aggtgaccaa aacggcgctg
    13261 gggagcggcc gtcagtcgcc ctcgtagcgc ttgaagatga ccgcgccgtt gtggccgccg
    13321 aggccgatgt tgtcgctcat cgcgtagcga atctccatct tcttggcctc accgtggacg
    13381 acgttgaggt tgatctccgg atcgtcgtcg cggtagttcg ccgtcgccgg tgccgtctgg
    13441 tgatacacgc tcaacacggt gaagacaccc tcgacggcgc cggccgcacc catcaggtgg
    13501 ccggtcagcg acttggtcgc ggaaatcgca acgttgggcg tgtggtcgcc gaatgttgcc
    13561 cagatggcct gtgcctcgcg cagatcgccg agtggcgttg atgtgccgtg cggattgatc
    13621 aggtcgacct ggtcggcggg cactccgtgg cgctcgagtg cctggatcat tgcccggcgg
    13681 gcaccgtcgc cctgctcgac cggctggatt agatcccacg catcggccgc ggagccatag
    13741 cccacgacct cggcatagat cgtcgcgccg cgcgccttgg cgtactcgag gtcctcgagc
    13801 accagcgcgc cgctgccctc gcccagcacg aagccgtcgc gcgtcttgtc gaacggccgc
    13861 gacacggtct ccacgggttc gccatcgcgc ggcttgccca tgccgcgcat attcgagaag
    13921 ccgatgtaga ccatctcgtg cagcgggttc tccgtcgcgc cggcgatcgc cgcgacaaag
    13981 tcgccgcggc ggatgccctc cgccgcctca ccgatgttgt gcgtgcccgt cgagcaggca
    14041 gtgacgatgc acatgtttga gccgcggatg ccggtctcga tcgcgatctg gccggacgcc
    14101 gtgtcgggca gcatgttggc gatgaagaac ggcgacacgg tgcgtgcgcc gctcgccttc
    14161 cacttctcga ggttctccat cagcaggtac ggcccgccgc cgcctgagcc gaagtagatg
    14221 ccgatgtcat cgcgattatc gtcggtgacc tcgaggccgg aatcggccac ggccatcttc
    14281 gacgcggcga caccaaggac gacgttgcgg tcagtccggc ggatggcctt gaagtccatc
    14341 cacttggtgc cgtcgaagtc gttcacctca ccgctgatct gggcctcgta gggcgtcgga
    14401 tcccagcgcg tgatgcggtg tagcccggtc ttggcattga ccaggctgtc ccagacagcg
    14461 tccttgtcct ggccgatggg gctgatgatg ccgaggccgg tgacggccac gcggcggttg
    14521 aagtcggggc gtcgaattcc catggtgcgg cggttgctcc ttacaagaac tcaggcataa
    14581 agaatcggtc gggtgccgac tgctcgtcga cggcgactgc ttccgattcg gagttgatgc
    14641 gcttgatcag attggtcagc accttgccgg ggccgacttc cacgaaccgg ccgactcccc
    14701 gcgcggacat ggcctcgacc gccttgaccc agtcgacccc gcgggtgaga tgctcgacca
    14761 gctcggcgcg gcactcctcg gcggtggtta ggggacgcgc gtcggcgttg gcgagcagcg
    14821 gcgcggatgg gtcattgaac gccaccgtgg cgaggacctc gcgcatcgca tccgcggcct
    14881 tgctcatcaa aggcgaatga gccgcgacac tgacgggaag gacgatcgcc cgcttggcgc
    14941 caagctcctt ggccttctcg gccgcgcctt cgaccgccgc gcgctcaccg ctgaccacga
    15001 tctgaccggg cgagttgcgg ttggcgatgg tgaatacgcc gagttgttga ccggccgatt
    15061 cgagggcgct gagtgcttcg tcgggcaggc cgatcaccgc ggccatcgaa ccatcctccg
    15121 cagatgcttg catctgcttg ccgcgctcac ggacgagacg cacgccgtcg gcaagtgaga
    15181 tgacgcccgc ggcgaccatc gcgctgtact ggcccatcga atggccggcg tagaaagccg
    15241 gcggcggaag ttcttgttcc cacgagcgta ggcgctcctc gatcgcgcgc aaatacgcga
    15301 tcgaacacgc caggatcgcc ggctgcgaat taatcgtctg gtcgagttgt tcggccggac
    15361 cgtcaaacat caggcgtgag agcggctcat cgagcaccat gtcgacagcg gccatagtcg
    15421 tggcaacacg ctcgctgctt tcggacaggg gcttgcccat gccgacgtac tgggacccct
    15481 gaccggggaa gacgaacgca aatgccactg cgcctgaacg gtagcagggg agtggcctca
    15541 ggccgcctga ttgcgtgcaa tgatgcacat atgtccgatg tcccccttga cgccgaagag
    15601 cgcgagctga gtccggccgt catcgccgcg cgcgaccaga tgca
//
LOCUS       DATBRX010000084         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3043857, whole genome shotgun sequence.
ACCESSION   DATBRX010000084 DATBRX010000000
VERSION     DATBRX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3043857"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VIF08_03120"
     CDS             complement(<1..530)
                     /locus_tag="VIF08_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8135006.1"
                     /translation="MTTPVALELSDADLTRIGTDIQRRIGVKTSRHEPLARFTTMRVG
                     GPADLFAEIHNLFELRAIVRFARARELPLFILGRGSDLVISDKGMRGLVVYNRAEGHK
                     VDGNRLTADSGLPMAKAATLCKNAGLSGLEFGLAIPGTIGGAVWANAGAHESDVRAVI
                     VEAGVLRADGEVTLSR"
     gene            complement(527..1669)
                     /locus_tag="VIF08_03125"
     CDS             complement(527..1669)
                     /locus_tag="VIF08_03125"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEY8135007.1"
                     /translation="MRILIAAGGTGGHVYPALAVARSLRERAPEAQIRWLGGRRGLEA
                     SIVPAEGYPLELLALRSLRTVDMSVHTVLDPIRLGASYPQALAKLARRRPDVVFTTGG
                     YLAIPVIAAAATLRIPSLMWEGNLVPGRSVRASARMASALAVSFAEACARLPGPCFVT
                     GTPIRSFAGRDKAAARAALGLEPDTPVMFVFGGSQQVRRLNRAVWDALPTLVATASVV
                     HMTGEPAYAEALKTREALAQEVRGRYKPFPFLRAEMTDALLAADLLVGRAGSSTLAEA
                     SALGLPMVVVPYPHASAHQVANARALAEAGAARIVADETFDAAALIDASGLLARPADL
                     QTMRDAARGFGRPGAANAVADLVMALAQRRPLPTVDAIERASRAAA"
     gene            complement(1581..>2183)
                     /locus_tag="VIF08_03130"
     CDS             complement(1581..>2183)
                     /locus_tag="VIF08_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8135008.1"
                     /translation="VVMSSQYQMARIQAFLDPWADPQGIGFHTVQGLLAMGMGGPFGI
                     GLGEGRQPGALHLPAAQNDFVFALVAQELGFLGGVAVIACYLVLAYRGIRIALSAPDT
                     FGALLAVGITGWLTFQAFINIGVVVVLLPITGITLPFVSAGGSSLLVSFAAVGILLSI
                     SRETLPRGTWNDADPDRRRWHRRPRLSGAGRRAQPARAGA"
BASE COUNT          359 a          769 c          778 g          277 t
ORIGIN      
        1 tcgcggctga gggtcacctc gccatccgcc cgcagcacgc cagcctccac gatgacggcg
       61 cggacatccg attcatgcgc gccggcgttg gcccacaccg ctcccccgat cgtccccggg
      121 atcgccagcc cgaactccag cccggacagg ccggcgttct tgcacagcgt cgccgccttg
      181 gccatgggca ggcccgaatc tgcagttagc ctgttcccat cgaccttgtg cccctcggcg
      241 cggttgtaga cgaccaggcc gcgcatcccc ttgtcgctga tgacgagatc cgagccacgg
      301 cccaggatga acaggggcag ctcgcgcgct cgcgcgaagc ggacgatcgc gcgcagctcg
      361 aagaggttgt ggatctccgc gaagagatcg gcgggaccgc cgacgcgcat cgttgtgaag
      421 cgcgcgaggg gctcatggcg cgacgtcttg acgccgatcc ggcgctggat gtccgtgccg
      481 attcgggtca ggtcggcgtc gctcagctcc aacgccacgg gcgtcgtcac gcggccgctc
      541 gcgaggcgcg ctcgatcgcg tccaccgttg gcagcgggcg gcgctgcgcg agggccatga
      601 ccaggtccgc cacggcgttg gcggcgccgg gccggccgaa gccgcgcgcg gcatcacgca
      661 tcgtttgcag atcggccggc cgcgccagca ggccgctggc gtcgatcagc gcggccgcat
      721 cgaaggtctc gtcggcgacg atgcgcgccg cgcctgcctc ggccagcgca cgcgcgttgg
      781 ccacctggtg ggcgctggca tgcggatagg gcacgaccac catgggcagg ccgagcgcgc
      841 tggcctcggc gagcgtcgaa gagccggcgc ggccgacgag caggtccgcg gcgagcagcg
      901 cgtctgtcat ctcagcgcgc aggaacggga acggcttgta gcggccgcgc acctcttgcg
      961 ccagcgcctc gcgcgtcttg agcgcctccg cataggcggg ctcgccggtc atatgaacga
     1021 cgcttgcggt ggcgacaagg gtgggcagcg cgtcccagac cgcgcggttg aggcggcgca
     1081 cctgctgcga gccaccaaag acgaacatca ccggcgtgtc cggctcgagc ccgagcgcgg
     1141 cgcgcgcggc tgccttgtcg cggccggcaa acgagcggat gggcgtgccc gttacgaaac
     1201 acggtccggg cagccgcgcg cacgcctcgg cgaacgacac ggcaagggcc gacgccatgc
     1261 gcgccgacgc gcgaacgctg cgtccgggca cgaggttgcc ctcccacatc aatgacggga
     1321 tgcgcaaagt tgccgcagcg gcgatgaccg gaatcgcgag gtaaccgccg gtcgtgaaga
     1381 cgacgtccgg ccggcgccgt gccagcttgg ccagcgcctg tgggtacgag gcgccgagtc
     1441 ggatgggatc gagcacggtg tggaccgaca tgtcaacggt gcgcagcgag cgcagcgcca
     1501 gcagctcgag cggatagccc tccgcgggaa cgatgctcgc ttcgaggccg cgccgcccgc
     1561 ccagccagcg gatctgcgcc tcaggcgccc gctcgcgcag gctgcgcgcg acggccagcg
     1621 ccggatagac gtggccgccg gtgccaccgg cggcgatcag gatccgcatc gttccaggtt
     1681 cctcggggca gggtctcgcg cgagatcgaa aggaggatac cgacagcggc gaagctcacg
     1741 agaagcgacg acccaccggc cgacacgaac ggcagggtga tgcccgtgat cggcagcagc
     1801 accacgacga cgccgatgtt gatgaatgcc tggaaggtca gccagccggt gattcccacc
     1861 gccagcaggg cgccgaacgt gtcgggcgcc gacagcgcga tccgtatgcc gcgataggcc
     1921 agcaccaagt agcacgcgat gaccgcgacg ccaccaagaa accccagctc ctgggcgacc
     1981 agggcgaaga cgaagtcgtt ctgcgcggcg ggcagatgca gcgcgccggg ctggcggccc
     2041 tcgcccaggc caatgccgaa cggcccgccc atacccatgg ccagcagtcc ctggacggtg
     2101 tggaagccaa tgccctgtgg gtcggcccag ggatcaagga atgcctggat ccgcgccatc
     2161 tggtactggc tgctcatgac gac
//
LOCUS       DATBRX010000085         3107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_697658, whole genome shotgun sequence.
ACCESSION   DATBRX010000085 DATBRX010000000
VERSION     DATBRX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697658"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1837
                     /locus_tag="VIF08_03135"
     CDS             <1..1837
                     /locus_tag="VIF08_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEY8135009.1"
                     /translation="FLVPTGDAKTIAEFSGKNLVQGEPDASSFPSGGIRATFEARGYT
                     AWDVTSPIFLQVEPNGVTMTIPTAYVSFTGEALDHKIPLLRSQAALGKQALRVLRWFG
                     NDRATRVLTAIGPEQEYFLVDRRLAEQRPDLLLTGRTLFGAPSPKGQELEDQYFGPIR
                     ERILAFMMDLDRELWRLGIPAKTRHNEVAPAQFEMAPVYEPTSVGSDHNMIVMSTMRR
                     LALQHGLMLLLHEKPFAGINGSGKHNNWSMATELGENLLDPGKDPHANAQFLAFLLAV
                     IRGVNVHADLLRAAIADAGNDHRLGANEAPPAIVSIFLGSQLEDVIHQIEQGPASASK
                     DGGSLDLGVDSLPGLPRDATDRNRTSPFAFTGNKFEFRAVGSSAPIYWPQTILNTAVA
                     DSLSDLADALDTLTPGDFDGLELILSDIARNHKQVLFEGNGYSPEWHAEAERRGLPNN
                     KTTVDALPALATDKAKTIFSRFGVLSERELAARVDIGWERYVKVGNIEANAALDIART
                     MIMPAAVRYLGQLLPAGAASRAAADLATRLGEKIDALGSAITKLEHAQHAAHETRSVQ
                     DEARAFVQEVIPAQNALRDIADELETIVADDLWPLPKYRELLFQY"
     gene            1856..2413
                     /locus_tag="VIF08_03140"
     CDS             1856..2413
                     /locus_tag="VIF08_03140"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY8135010.1"
                     /translation="MARCWWAGSDPLMVAYHDDEWGRPSSDERHLFEMLTLEGAQAGL
                     SWQTVLRKREGYRRAFGGFDVDVVARYTEADEARLLADAGIVRNRAKIRAAIGNAQAA
                     LRVRDEFGSLHAYFVGMVPQPPAKLPHTAEPGTIPITTAVSDALSKDLRKRGFKFVGS
                     TIVYSFMQAVGLVDDHLPMCFRYER"
     gene            complement(2414..2860)
                     /locus_tag="VIF08_03145"
     CDS             complement(2414..2860)
                     /locus_tag="VIF08_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135011.1"
                     /translation="MRKRLVLIWTPVVLGLALLLPATTLAGQPATYVVLKNSCSNSGA
                     GQGYGKSVIQIQQNEYATSGVRQFRQRAWAQMKWGGSWHIVDSFNWQYSSKFANSAAD
                     HSFTFKFIEHWSSDHLSYPARLKWRGEWLNGNGALLFYKNVFSSSC"
BASE COUNT          530 a         1066 c         1000 g          511 t
ORIGIN      
        1 gttcctcgtg ccgacgggcg acgccaagac catcgccgag ttcagtggca agaacctcgt
       61 ccagggcgag ccggacgcgt cgtccttccc gagcggtggc attcgtgcca ccttcgaggc
      121 gcgcggctac accgcgtggg atgtcaccag cccgatattc ctccaggtcg agcccaacgg
      181 cgtcaccatg accatcccga ccgcctacgt ttcgttcacc ggcgaggcgc tcgatcacaa
      241 gatcccgctg ctgcgctcac aggccgcgct gggcaagcaa gcgctgcgcg tcctgcgctg
      301 gttcggcaat gatcgcgcca cccgtgtgct gacagccatt gggccggagc aggagtactt
      361 cctcgtcgat cggcgcctgg ccgagcagcg gcccgacctg ctgctcacgg gccgcactct
      421 ctttggcgcc ccgagcccca agggccagga gctcgaggac cagtatttcg gcccgattcg
      481 ggagcgcatc ctcgcgttca tgatggatct cgaccgcgag ctgtggcgcc tgggcatccc
      541 cgccaagacg cgccacaacg aggtcgctcc ggcgcagttc gagatggcgc cggtgtacga
      601 accgacatcg gtcgggtccg accacaacat gatcgtcatg tcgacgatgc gcaggctggc
      661 cctccagcac gggttgatgc tgctgctgca cgagaagccg tttgccggaa tcaatggatc
      721 tggcaagcac aacaactggt ccatggcgac cgagctgggc gaaaacctgc tcgacccggg
      781 caaggacccg cacgccaacg cccagttcct ggcgttcctg ctggcggtca tccgcggcgt
      841 gaacgtgcac gccgatctcc tgcgggcggc cattgccgac gcgggcaacg accatcgcct
      901 gggcgccaac gaggcgccac cggccatcgt gtcgatcttc cttggctcgc agctcgagga
      961 cgtcatccac cagatcgagc aaggccccgc gtcggcgtcc aaggacggcg ggtcgctcga
     1021 cctgggagtc gattcgctgc cgggtttgcc gcgcgatgca acggaccgca accgcacctc
     1081 gccgttcgcc ttcacgggca acaagttcga gttccgcgca gtgggctcgt cggcgcccat
     1141 ctactggccg cagacgatcc tcaacacggc ggtcgccgat tccctgtccg atctggccga
     1201 cgcgctggac acgctcacgc cgggggactt cgacggcctc gaactgatcc tgtccgatat
     1261 cgcgcgcaac cacaagcagg tgctgttcga gggcaacggc tactcacccg agtggcacgc
     1321 ggaagccgag cgacgcggac tgcccaacaa caagaccacc gtcgacgcgt tgcccgcgct
     1381 ggccaccgac aaggccaaga cgatcttttc gcggttcggc gtcctgtccg agcgtgagct
     1441 cgcggcgcgc gtcgacatcg gttgggagcg ctacgtcaag gtcggcaata tcgaggccaa
     1501 cgcggcgctc gatatcgcgc gcacgatgat catgcccgcg gcggtgcgct acctgggcca
     1561 gctcctgccg gccggtgcgg cgtcacgcgc tgcggcagac ctggccaccc gtcttggcga
     1621 gaagatcgac gcactgggct cggcgatcac caagctcgag cacgcgcagc acgcggcgca
     1681 cgagacgcgc tccgtccagg atgaggcgcg ggcgttcgtc caagaggtca tcccggcgca
     1741 gaacgcgctg cgcgatatcg cggacgagct cgagaccatc gtcgccgacg acctgtggcc
     1801 gctacccaag taccgcgagc tgctattcca gtactgagcc cgcgctatgg tgagcgtggc
     1861 gcgctgctgg tgggccggca gcgatccgtt gatggttgcc taccacgacg acgagtgggg
     1921 ccgcccgtcg agcgacgaac ggcatctctt cgagatgctc accctggagg gcgcccaggc
     1981 gggcctgtcg tggcagaccg tcctgcgcaa gcgcgagggc taccggcgtg cgttcggcgg
     2041 ctttgacgtc gacgttgtgg cgcgttacac cgaggcggac gaagcgcgtc tgctcgcgga
     2101 cgcgggtatc gttcgcaatc gcgccaagat cagggccgcc atcggcaacg cccaggcggc
     2161 gctgcgcgtg cgtgatgaat tcggctcgct ccacgcctac ttcgtcggca tggtgccgca
     2221 gccgccagcc aaactcccgc ataccgctga gccgggcacg atcccgatca cgacggcagt
     2281 ttcagacgcg ctgtcaaagg acctgaggaa gcgcggcttc aagttcgtcg gctcgacgat
     2341 cgtgtactcg ttcatgcagg cggtcgggct cgtcgacgac cacctgccga tgtgcttccg
     2401 ctacgagcgc tagctagcag ctcgagctga agacgttctt gtagaagagc agcgccccgt
     2461 tgccgttgag ccattcgccg cgccacttga gccgcgccgg gtagctgagg tggtctgacg
     2521 accaatgctc aatgaacttg aaggtaaagg agtggtcggc agcgctattc gcgaacttgg
     2581 acgagtactg ccagttgaac gaatcgacga tgtgccacga cccgccccat ttcatctgcg
     2641 cccaggcacg ctggcggaat tgcctgacgc ctgacgtggc gtactcgttc tgctgaatct
     2701 ggataacgga cttcccgtag ccctgtccgg cgcccgagtt gctgcacgag ttcttcagca
     2761 caacgtacgt cgctggttgc ccggccaggg tcgttgccgg aagcaacagg gccaggccaa
     2821 gaacgacggg cgtccaaatc agaacgagcc gtttgcgcac cgctgaactc cctctagccc
     2881 atcctgcgtc aagcgactgg gctgcggcga atggtcgatc tcgacgtcgt cccccgtcaa
     2941 taccagaggg ccgccccttt cttggctcag gcgcccccgc ggcgggcgcc ggtggcctcg
     3001 ccgccgtcga cgccgatcac attgccggtc atccagtaca ggtgcggcga cgcgagcgcg
     3061 atgagcgcct caccgacgtc ctcgggccgc gtcaggcggc caccggg
//
LOCUS       DATBRX010000086        14943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1144782, whole genome shotgun sequence.
ACCESSION   DATBRX010000086 DATBRX010000000
VERSION     DATBRX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144782"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VIF08_03150"
     CDS             <1..949
                     /locus_tag="VIF08_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEY8135012.1"
                     /translation="RRRRVKVLVAEPLGQAGLDRLAQEHEVDVRLNLAREELLGLLPD
                     YDALIVRSQVQVDGAAIAAGTRLKVVGRAGVGVDNIDVAAATSAGIAVVNAPTANTLA
                     AAEHTMALMLALARRVAVADASVRRGEWRRADFMGTELGGKTLGILGLGRVGLAVADR
                     ARAFAMNLLGSDPLVSAEAAATYGVRLVDVDELLRESDVVTLHVPLVAQTRGLINAPR
                     LGRMKRGALLINVARGGVVDESALAVALTSGQLGGAAIDVFAHEPLGDSPLLSAPNTV
                     LTPHLGASTAEAQARASLEVVQGVLDVLAGRPATYQVNR"
     gene            complement(946..3351)
                     /locus_tag="VIF08_03155"
     CDS             complement(946..3351)
                     /locus_tag="VIF08_03155"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEY8135013.1"
                     /translation="MSHNRAQQVVVDDASEDYDADWPFTPREGWLSVIALVVMITAVA
                     VAIDDALWAGFSVGTRSSQTGFLPAAALASVLLGSYLAKTTLSTRRINLIGILGGAAA
                     MLFFVSNAVSRSATLDARLHDLNTSVSVFVHDVFVLGTRSTESSIFLLIMGAVVWGAG
                     LFCAITVFRRHRPLPAIVLAGTLLLLNVLLTTREQYLHLLVFAAAALLLAVRLNLREQ
                     ADGWRVRGTRNVSEISASFMRGGAVLVAVAVIASSFLAANASSAPLARAWSTWDDDLL
                     SIGYTINRWLGGVTGTARGPNVLFTPSQTIRDFWQSSSEEVFSATVSDGIGRRWRGAT
                     YDSFDGRSWQQLDRQAKLIQAGDLLFAGTPEAVARAGRAEITVQVMPIDYGGDVFVAP
                     AQPLDVSQPTELTTRGAGGAFVSGSLTYGIEPGVSYSVTSLVRAEGALTATRLASAGT
                     EYPEWVKPYLDIRPDSMGDAVADTAAKIVAGLPSAKRDPYHVALAIQDYLYSSGGFSY
                     TTDIRGECAGEPLLDCFVRIKKGYCEYFATAMVMMLRAQGVPARYVLGYLPGQQQEDR
                     SWRVTRGAAHAWVEVYFPNYGWVEFDPTPGNTENGQQPTRLPIGDPPPQGSPGPDNPD
                     PQGPDNLDNDCPFNEKTCVDVDRGQGPTGAPAAPGGGGPPQPLLIAALLVLVATALGA
                     WIAWRRIPSTQPELAYSGITRLASRLGYAPRPSQTTYEYAARLADLVPVARPDLELLA
                     TAKVEAVYGRRAAGSALLVRIGAAYRHARTGLLRLLFRRPKFRLGRGLRSAKGPGATS
                     ERR"
     gene            complement(3348..4640)
                     /locus_tag="VIF08_03160"
     CDS             complement(3348..4640)
                     /locus_tag="VIF08_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8135014.1"
                     /translation="MVRRIQLLVAAIVLVVAALSTGAQLLFFLVYLGIIVVGGSYVVT
                     RFGLADLEAGYVLDRLHARAGDMLRATYTVRNTSRLPKLWLEVHNPTTLPVALPGQAI
                     TIGPRGERTWSVHVPLTRRGHFRVDPLALRTGDPLGLFESNAAIGGYSTVVVYPRVEV
                     LPGWRMPPAFVEGSHADKVRTPHVTPTATSIREYLPGDAYNRIHWKASARQGQLLVKE
                     FELEQTADVWIFLDLHSPSHTGSGDESTLEYGVRVAASIAARALVENRKVALTASGRR
                     ISPIPADRGPRQYQKIMETLAAVAADGGRPLHEVLVENVGRLRRGMTALVVTASLDAH
                     WVLPLNGLRRRGVETFVIMLDAPAFAGYGQQVGQHPPSEQALNAQRTAREESLRAQRA
                     LRHALAEHDLRWLNVEPGEPLASQLVTRGPRPVLVAAR"
     gene            complement(4651..5625)
                     /locus_tag="VIF08_03165"
     CDS             complement(4651..5625)
                     /locus_tag="VIF08_03165"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8135015.1"
                     /translation="MTIAQTTDKVIANVEKVIVGKHAEVRLALVALLCQGHLLIEDVP
                     GTGKTSLAKAIAKSLGCTFRRIQFTPDLLPSDVTGLSIFNQKTHEFEFRPGPIMAQIV
                     LADEINRATPKTQSALLECMEERQATIDGVTYPMPDPFLVIATQNPIEYEGTFALPEA
                     QLDRFMLRIQLGYPLPAEEVRILDEHKSSRPIDDLGEVVSGKELQEMQAAVRDIYVDA
                     QVSEYIVRLVTATRNHPDVYLGASPRGSLGLYRAGQAFAALSGRDYVIPDDIKALAVP
                     ALAHRTIVKTAATVRDVDSSLIIRELLDSVPVEVHGVRSERPAAVRRA"
     gene            complement(5762..7336)
                     /locus_tag="VIF08_03170"
     CDS             complement(5762..7336)
                     /locus_tag="VIF08_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8135016.1"
                     /translation="MSGPRLRLQQLRLHLRPRLRTRGAVPSLPAGTLGAPPRVGRSLL
                     SLSVALALAYAALAGGLGYWQVVQAQALTADPLNPLTLAAARSAPRGTIYDVNGVVLA
                     SNVRGPNGERLRQYGYSIAAPVIGYASPIFGTAALERTYDAQLTGLISLRPGDELLRK
                     FRGQPYDPSDLHLSLDIQLQRVAADLLGDQKGAIVAIEPTSGRILALVSSPTYNPNRV
                     VDPDDGRRYMASLLERDDSPLLDRATQGLYVPGSVFKIVTSIAGLDSGSITAQTTYEN
                     QPDEFETGFLVDGFRIHDFPRDVQTDHPLDYYEALEVSSNIWFAHAGLDTGAANLTSW
                     AARLGFGARIPFDLPTSPSQINGGDGSLNGFRDRVELANAAYGQAEVLVSPLQMALVA
                     ATIANNGTLMRPSIVDSLTSSDGGVTTLAPQTWTQVCSFGTAQIIGDAMQQAVEGRFA
                     FAGAAKVPGVPTAGKSGTAEIGPDAAPHSWFIGFAPADAPRIAIAVIVEGGGLGSQRA
                     VPMGGRLMSAYLGVPR"
     gene            complement(7333..8763)
                     /locus_tag="VIF08_03175"
     CDS             complement(7333..8763)
                     /locus_tag="VIF08_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8135017.1"
                     /translation="MTAALTRWGIRPRPRWLELRLLVVVGLALVIGWTSLESFRSGAI
                     GPGDMSLLAIYLVAVGLIHLAFVITGRRMDEILFPAAALLAGLSLLIMNRLPQDLVVQ
                     SVAGLELPLGKLQLVWILLGFTLLGVTAVVVRSDGWLRRYKYTWAAAGIALLLLVFVF
                     GSIVGGARLTLHIGPFAGQPSELLKVILVVFLAAYLAENRALLAMSSTRVGAFNLPPL
                     PYLLPMLAMWGLALAIVIVQRDLGAALLYFLVFLGLLYVATRRIAYVLFGIALFLAGG
                     ALLYVLFTHVQDRINIWIDPFADAQGAGYQVIRAMYAYGRGGILGTGLGAGLPSVGDV
                     PSIPAIHTDFVFAALAEELGMLGALAILGLYLIIAERGFRIAAAATDDFRALLATGLT
                     LVIVVQAAIITGGNLRVLPLTGVTLPFISYGGSSLLVNGAVIGLLLALSDRGTEHKAP
                     DARVNVVSRAAQRARAVGGRIEEALS"
     gene            complement(8760..9200)
                     /locus_tag="VIF08_03180"
     CDS             complement(8760..9200)
                     /locus_tag="VIF08_03180"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8135018.1"
                     /translation="MDVFTLSLWALRLGFVLVIYIFFFFVARALWRDLRAGVAGAGRP
                     LARLLVVGSPEGRPPAGTSISLDAVNSLGRDVNNSIVLDDSFVSAEHALLTFRGRAWY
                     VEDRGSTNGTWLNGQRIEGLAPLGFGDEVQIGQVRMRLERPRQE"
     gene            complement(9298..10107)
                     /locus_tag="VIF08_03185"
     CDS             complement(9298..10107)
                     /locus_tag="VIF08_03185"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HEY8135019.1"
                     /translation="MAGPLTAVERFFERLFERPAARLFQERVERVQIQRGIERAMEAE
                     RVVRERRAFVPAHYRVLLSTADAAALDGDMASLTRDLAEQVRVYARAHQYTLQARPSV
                     EVIGSNAVASGDVRVYADRAPAPTRNAPTAPPEPAPVPVNADGDDAPDVDDDAATVPG
                     ATAVFAAPRPNTPRAQLAVRSPGQPVSRLNVRPGTIRLGRALDNEIVLADDKVSRHHG
                     QIGIRLGMLVYTDLGSTNGSYLNGALVTEIALGPGDVLQIGSSTVTVEPAP"
     gene            10231..11154
                     /locus_tag="VIF08_03190"
     CDS             10231..11154
                     /locus_tag="VIF08_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135020.1"
                     /translation="MDVFCERCGRPQPKPEVAAPRSAGLLARRVLDAVGITENQPAPT
                     EDWLRLCFNCRGYSCPACWNETAGVCQTCEPLPEPIVEVAPEPLPEPVVIEAEPVVPE
                     PLVAVPEPLVAAPEPMVATPEPMVAETVPEVEAEPLPVLFAEAELEPEIELALEREPV
                     EVLDADPVFAVEAESESEPEPERVAAAEPEPEPVAAAEPEPEPEPVERPRMPVLPLPR
                     PRPADAPAIPLPPLPAYPVAPQIAFNPQVSVAASSQTSAPAYEAHLLLAPPPQLAPNA
                     IAAGLRPCPNCHLTLSARAHFCRRCGAAQAH"
     gene            complement(11151..12017)
                     /locus_tag="VIF08_03195"
     CDS             complement(11151..12017)
                     /locus_tag="VIF08_03195"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8135021.1"
                     /translation="MIYRLRARVDALVTGITGHRRVRPVLDVINTANDTGATLFAAAL
                     AFGTMFAFIPLVLLVSGVIGWLIEDPLQRASLLDQLISYVPPLADFFRSSLEGAVAGR
                     GALSIIGLIGLLWGASAFYGVLDEVMRRIFSGGGLRNEFSRRIRGFATIVILLLVIVG
                     TVSLGGLWATLDELVGELSVWRYAAPLLSLVVMVLIALVVYLVVPTAPPSLRAAFWPA
                     VAAGFGIGLLTNLFSLLAPLLIGGMAGFGVIATVFGALVWLNFSYQILLFGAAWARVR
                     RDREQYAGTTAA"
     gene            complement(12049..12345)
                     /locus_tag="VIF08_03200"
     CDS             complement(12049..12345)
                     /locus_tag="VIF08_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY8135022.1"
                     /translation="MPLYDYICDNGHIVEVMHGVNDSGPAKCERCGAEMRKLLSTPAI
                     VFKGSGWAKKDRSSKPAPRDKPATDTEAKPSTATDGQSAKPDTATDTASSDSKP"
     gene            complement(12350..13084)
                     /locus_tag="VIF08_03205"
     CDS             complement(12350..13084)
                     /locus_tag="VIF08_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8135023.1"
                     /translation="MPIPFIGRKKKPVRAAPEPVRIPPDQIDVTSQSIKYQYYSMSSH
                     GVRTPGTHDVPQRLPALLSGFAISPVELIDPLPIDFEDAAPSLVRPEAALQWINAHHG
                     RSPLGRQALFVFESLDVVDLAYETFALALLHGELDGEGFPRYDAIFGGPVSYWDESSG
                     DLVVRFLLGWGGEGIRGDTQRIAQRLLARLMGNLLVSHDATELGAVARPVAVSGARHE
                     PCSHCGFPAIDRRAQYCPKCGMRPRA"
     gene            complement(13087..>14943)
                     /locus_tag="VIF08_03210"
     CDS             complement(13087..>14943)
                     /locus_tag="VIF08_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY8135024.1"
                     /translation="VKITPAHDQDDFETGKRHGLPIIDVMTDDGHINENGASFAGLTL
                     NDARTRILDELRESGDLAGEQPHDMVLGRCERSNDVIEPRLKTQWFVRTRPMADKAMA
                     AVNEGRTTFVQEHHRKVFFDWMEKIHDWNVSRQLWWGHRIPAWYCPDDHITVSDAADG
                     PGGCDLCGRPAAELRQEEDIFDTWFSSGLWPWSTLGWPEETADLAKYYPTTVMETGYD
                     ILFFWVARMMMLGEWLTGSEPFRYVYLHGIVRDPLGQKMSKSKGNVLDPLGVINELGA
                     DALRFALLHGPEPSQDQKMSRPRLEDARNFANKIWNAARFVLSARPNEMPPDFSLGLI
                     DTDQLGPAEHWIVQRCESTLAAAEAAYANFEFGEVARLLYDAIWNDFCDWYVELAKIG
                     LGDASASAERKRAIWSTLTWVLDRYLRLLHPLMPMLTEEIWGKLPHRADDPELLIVAP
                     WPVAADAAATADPARASGAAGLIELVTAMRSARAESGIEAGDILPAQIWLANGPARDA
                     YGDMAAAVARLARVTATLIDDRAALAEGVAVVSTVAEARLTQSATQLERERARLDKEL
                     RELRAQLDAARARLSDANFIGRAPTAVVNEARRRAAELEAQVAALTARRGEV"
BASE COUNT         2407 a         5031 c         5273 g         2232 t
ORIGIN      
        1 tcgccgccgg cgggtgaagg tccttgtcgc tgaacccctc ggacaggccg ggctcgaccg
       61 gctggcccag gagcacgagg tagacgttcg gctcaacctg gcgcgtgagg agctgctggg
      121 cttactgcct gactacgacg ccctgatcgt gcgcagccag gtccaggtcg acggcgcggc
      181 gatcgcggcc ggaacgcgac tcaaggtcgt tggccgcgcc ggggtcggcg tcgacaacat
      241 tgatgttgcc gcggccacca gcgccggcat tgcggtggtc aacgcgccaa cggccaacac
      301 cctcgctgcg gccgaacaca ccatggcgct gatgctggcc ctcgcgcgcc gcgtagccgt
      361 tgctgacgcg tcggtgcggc gtggggagtg gcgccgcgcc gacttcatgg gcacagagct
      421 gggcggcaag acgctcggca ttcttggcct tggccgggtg ggcctggcgg tagccgatcg
      481 cgcccgcgct ttcgcgatga acctcctggg tagtgacccc ttggtcagtg cggaagccgc
      541 cgcgacttat ggcgtgcgcc tggtcgatgt agatgagctg ctccgggagt cggacgtcgt
      601 caccctgcac gtgccgctgg tcgcacagac gcgcggcttg atcaacgccc cgcgcctggg
      661 gcgcatgaag cgcggagcgc tgctcatcaa cgttgcccgt ggtggcgtcg tcgacgagtc
      721 agcattggcg gtcgcgctga cgagtggcca gctgggtggc gccgcgatcg atgtcttcgc
      781 gcacgagccg ctgggcgact cgccgctgtt gagcgccccc aacaccgtac tgacgccgca
      841 cctgggcgct tcgactgccg aggcacaggc acgagcgtcg ctggaagtgg tccagggtgt
      901 gctcgatgtc ctcgccggtc gtccggcgac ctaccaggtg aaccgctagc gccgctctga
      961 ggtggcccct ggtcccttgg cgctgcgcaa tccgcggccc aggcggaact tgggccgcct
     1021 gaacagcagg cgcagcaacc cggtgcgcgc gtggcgatag gctgcgccga tccgcaccag
     1081 cagcgcagag cccgccgctc gacggccgta gacggcctcc accttggccg tggcgagcag
     1141 ctccagatcg ggccgcgcaa cgggcacgag gtcagccagg cgcgccgcgt actcgtaggt
     1201 cgtctgcgac ggccgtggcg cgtaacccag gcgcgacgcg aggcgggtga tgccgctgta
     1261 ggcgagctcc ggctgcgtgg acggtatgcg gcgccacgca atccacgccc ccagggccgt
     1321 cgcgaccagc accagcaacg ccgctatcaa gagcggctgc ggcggaccgc ctccgcctgg
     1381 agcagcgggc gcacccgttg gcccttgccc tcgatccacg tcgacgcagg tcttctcgtt
     1441 gaaggggcag tcgttgtcaa ggttgtcggg gccctgcgga tccgggttgt ccgggccagg
     1501 tgagccctgg ggaggcgggt cgccgatcgg caggcgcgtt ggctgctggc cgttctccgt
     1561 gttgcccggc gtcggatcga actcgaccca gccgtagttc gggaaataga cctcgaccca
     1621 tgcatgcgcg gcgccgcgcg tgacccgcca ggaccggtcc tcctgctgtt gaccgggcag
     1681 atagccgagt acatagcgcg cgggaacacc ctgggcgcgg agcatcatca ccatcgcggt
     1741 ggcgaagtac tcgcagtagc ccttcttgat gcgcacgaag cagtccagga gcggctcgcc
     1801 cgcgcattcg ccgcggatgt cggtcgtgta tgagaagccg cccgacgaat acaggtagtc
     1861 ctggatcgcc agggccacgt gataagggtc gcgcttggcc gatggcaggc cggcaacgat
     1921 cttggctgcc gtgtcggcga cggcgtcgcc catcgagtcg ggccggatgt cgaggtacgg
     1981 cttgacccat tcgggatact ccgtgcccgc actcgccagg cgcgtcgcgg tcagcgcgcc
     2041 ttctgctcgg acgagcgacg tgactgagta gctgacgccg ggctcgatgc cgtaggtcag
     2101 gctgcccgac acgaacgcgc caccggcgcc acgcgtcgtg agctccgtcg gctggctaac
     2161 gtcgaggggc tgcgccggcg cgacgaagac atcgccgcca tagtcgatcg gcatcacctg
     2221 gacggtgatc tcggcgcggc cggcccgtgc cacagcttcc ggcgtacccg cgaaaaggag
     2281 atcgccggct tggatcagct tggcctggcg atcgagctgc tgccacgagc ggccatcgaa
     2341 cgagtcgtag gtcgcgcctc gccaccgccg gccgatgccg tcgctgaccg tggcgctgaa
     2401 gacctcttcc gacgaggact gccagaagtc gcggatggtc tggctgggcg tgaacagcac
     2461 attcgggcca cgcgcggtgc cggtgacacc accgagccag cggttgatcg tgtagccgat
     2521 gctcagcagg tcgtcgtccc aggtgctcca ggcgcgggcg agcggggccg agctggcatt
     2581 ggccgcgagg aagctgctgg cgatgaccgc gacggccacc aacactgctc cgccgcgcat
     2641 gaacgatgcc gatatctccg acacgttgcg cgtgccgcgc acacgccagc cgtcggcctg
     2701 ctcgcgcagg ttgagccgca cggccaggag gagcgccgcg gcggcgaaga cgagcaggtg
     2761 caggtattgc tcgcgcgtcg tgagcaggac gttgagcaac agcagcgtgc ctgcaagcac
     2821 gatcgctggc agcggccggt ggcgtcggaa cacagtgatg gcgcagaaca ggccggcgcc
     2881 ccacacgacg gcgcccatga tcaagaggaa gatcgaggac tcggtcgagc gcgtgcccag
     2941 gacgaacacg tcgtggacga agaccgacac ggatgtgttc aggtcgtgca gccgggcgtc
     3001 gagtgtggcc gagcggctga cagcgttgct gacgaagaac agcatggcgg cagccccgcc
     3061 caagatgccg atcaggttga tgcgccgcgt cgatagcgtg gtcttggcca ggtaggaacc
     3121 gagcaacacg ctcgccagtg ccgccgcagg caggaagccg gtctgggacg atcgcgtacc
     3181 gacgctgaac ccggcccaca gggcatcgtc gatcgctacg gcgacggctg tgatcatgac
     3241 taccagggcg atgaccgaca gccagccctc gcgcggcgtg aacggccagt ccgcgtcgta
     3301 gtcctcggac gcgtcgtcga ccacgacctg ctgcgctcgg ttatggctca tcgcgcggcc
     3361 accaggaccg gccgcgggcc gcgcgtgaca agttgcgagg ccagcggctc cccgggctcg
     3421 acattgagcc agcgcaggtc gtgctccgcc agcgcgtggc gcagggcgcg ctgcgcacgc
     3481 aggctctcct cgcgcgcggt tcgctgcgcg ttcagcgcct gctccgaggg cgggtgttga
     3541 ccgacctgct ggccatagcc tgcgaaggcc ggggcgtcga gcatgatcac gaacgtctcg
     3601 accccgcgcc ggcgcaagcc gttgagcggc agaacccagt gcgcgtcgag cgaggcggtg
     3661 acgaccagcg ccgtcatgcc ccggcgcagc cgcccgacgt tctcgacaag cacttcatgc
     3721 agcggccggc cgccgtcggc cgcgaccgca gcgagcgtct ccatgatctt ctggtactgg
     3781 cgcgggccgc gatcggccgg gatggggctg atgcgcctgc ccgacgcggt cagcgccacc
     3841 ttgcggttct cgacgagcgc ccgcgcggcg atggaagcgg cgacccggac gccgtattcg
     3901 agcgtggatt cgtcaccgct gcccgtgtgg gagggtgagt gcaggtccag gaagatccag
     3961 acatcagccg tctgctcgag ctcgaactct ttgaccagca gctggccctg gcgcgcactc
     4021 gccttccagt ggatgcggtt gtaggcgtcg cccggcaggt attcgcggat gctggtggcg
     4081 gtcggcgtga cgtgcggtgt acgtaccttg tccgcgtgac tgccctccac gaatgcgggc
     4141 ggcatccgcc agccgggcag cacctcgacg cgcggataca cgacgacggt cgagtagccg
     4201 ccgatcgccg cgttggactc gaacaagccg agtgggtctc cggtgcgcag cgcgagcgga
     4261 tccaccctga agtggccgcg ccgcgtcagg ggcacgtgga ccgaccaggt gcgttcgccg
     4321 cgcggcccga tcgtgatcgc ctggccgggc agagcaacgg gcaatgtcgt ggggttgtgg
     4381 acctccagcc acagcttggg cagacgactc gtgttgcgga cggtgtacgt ggcgcggagc
     4441 atgtcgccgg ccctggcgtg caaacggtcg aggacatagc cggcttcgag atcggccagc
     4501 ccgaagcgag tcacgacgta cgagccgccg acgacgatga tgcccaggta tacgaggaag
     4561 aacaggagct gggcaccagt cgacagcgcg gcaacgacca gcacgatcgc cgcgaccagt
     4621 agctgaattc ggcggaccat cgccgtctac ctaggcccga cggaccgccg caggtcgctc
     4681 ggagcgcacg ccgtgcactt cgacaggcac gctgtcgagc agttcgcgga tgatcagcga
     4741 cgagtcgacg tcgcgcaccg tcgccgccgt cttgacgatg gtccggtggg cgagcgccgg
     4801 caccgcgagt gccttgatgt catcggggat cacgtaatcg cgaccggaca gggcggcgaa
     4861 ggcctgcccg gcgcggtaca gccccagcga gccgcgcggc gaagcgccga gatagacgtc
     4921 gggatggttg cgcgtcgcgg tgaccaggcg aacgatgtac tcgctcacct gcgcatcgac
     4981 gtagatgtcg cgcaccgctg cctgcatctc ctgcagctcc ttgccgctga cgacctcgcc
     5041 gagatcatcg atgggccgcg agctcttgtg ctcatcgagg atgcggactt cttccgcggg
     5101 cagcgggtag ccgagctgga tgcgcagcat gaagcggtcg agctgggctt cgggcagagc
     5161 gaacgtgccc tcgtactcga tcgggttctg cgtagcgatg acgaggaacg ggtcgggcat
     5221 ggggtaggtg acgccgtcga tggtcgcctg gcgctcctcc atgcactcca gcagcgcgga
     5281 ctgggtcttg ggcgtggcgc ggttgatctc gtcggcgagc acgatctggg ccatgatcgg
     5341 cccgggccgg aactcgaact cgtgggtctt ctggttgaag atcgacaggc cagtgacgtc
     5401 cgagggcagc aggtcgggcg tgaactggat gcgccggaaa gtgcagccga ggctcttggc
     5461 gatcgccttg gcgagcgatg tcttgcccgt gccgggcacg tcctcgatca gcagatggcc
     5521 ctggcaaagc agggcgacga gcgccaggcg cacctcggca tgcttgccga cgattacctt
     5581 ctcgacgttg gcgatgacct tgtcggtcgt ctgcgcgatg gtcattgtgc gagcggcccc
     5641 tcctgcctgg tcagacgcgt gcagcttgcc ctctgccgtc tgggccccgg tcaggaaagg
     5701 cttggcagat agtacgcccg agtgatccaa gcggttcccg atctacccag agtgccgaga
     5761 gctaccttgg gacgcccaga taggcgctca tgaggcgccc gcccatgggc acggcacgtt
     5821 gcgagcccag gccgccgccc tcgacgatca cggcgatcgc gatgcgcggc gcatctgccg
     5881 gggcgaagcc gatgaaccat gagtgcggtg cggcgtcggg cccaatctcc gcggtgccgc
     5941 tcttgccggc tgtcggcacg cccggcacct tcgcggcgcc ggcgaacgcg aagcgcccct
     6001 ccaccgcctg ctgcatcgcg tcgccgatga tctgtgccgt cccgaaagag cacacctgcg
     6061 tccacgtctg cggggcgagg gtcgtcacgc cgccgtcgct ggaggtgagc gaatcgacga
     6121 tcgacggccg catcagcgtg ccgttgttgg cgatggtcgc cgcgaccagg gccatctgga
     6181 gcggcgacac gagtacctcc gcctggccgt aggccgcgtt ggccagctcg acccgatcgc
     6241 gaaagccgtt cagcgatcca tcgccaccgt tgatctggct gggcgaggtg ggcagatcga
     6301 acgggatgcg cgcgccgaag cccagccgtg ctgcccagga cgtgaggttg gccgcgccgg
     6361 tgtcgagccc ggcatgcgcg aaccagatgt tcgacgacac ctccagcgcc tcgtagtagt
     6421 ccagcgggtg gtcggtctgc acgtcgcgcg ggaaatcatg gatgcggaag ccgtcaacca
     6481 ggaaaccggt ctcgaactcg tcgggctggt tctcgtatgt cgtctgcgcg gtgatgctgc
     6541 ccgaatccag gccggcgatc gacgtcacga tcttgaacac cgatccgggc acatacaggc
     6601 cctgcgtggc ccggtcgagc agcggcgagt cgtcgcgctc gagcaggctc gccatgtagc
     6661 gccggccgtc gtcgggatcg acgacgcggt tggggttgta ggtgggactg ctgaccaggg
     6721 cgaggatgcg gccgctggtg ggctcgatgg cgacgatcgc gcccttctgg tcaccgagca
     6781 ggtctgctgc gacgcgctgt agttggatgt ctagcgacag gtgcaggtcc gacgggtcat
     6841 acggttggcc gcggaacttg cgcagcagct cgtcacccgg cctgagcgag atgagcccgg
     6901 tcagctgcgc gtcgtacgtg cgctcgagcg cggcggtgcc gaatatcgga ctggcgtagc
     6961 cgatgaccgg cgcggcgatc gagtacccgt actggcgcag ccgctcgccg ttggggccgc
     7021 ggacgttgct cgccaggacc acgccgttga cgtcgtagat ggtgccgcgc ggcgccgaac
     7081 gggccgcggc cagggtgagc gggttgagcg ggtcggcggt gagcgcctgg gcctgcacga
     7141 cctgccagta ccccaagcca ccggccagcg cggcgtacgc cagcgcgagc gcgaccgaca
     7201 gcgagagcag gctgcgcccg acgcgtggcg gagcgccgag ggtgccggcg gggagcgacg
     7261 gaacggcgcc gcgcgtccgc aaccgcggcc gcagatggag tcggagctgc tgcagacgga
     7321 ggcgcgggcc gctcacgaca gtgcctcttc gatccggccg ccgaccgcgc gagcgcgctg
     7381 cgcggcccga gaaacgacgt tcacccgcgc gtccggcgcc ttgtgctcag tgccgcggtc
     7441 gctcaacgcg agcagcaggc cgatgaccgc gccgttgacc agcagcgatg agccgccgta
     7501 cgagatgaag ggcagcgtca cgccggtcaa cggcagaacg cgcaggttgc cgccggtgat
     7561 gatcgccgcc tggacgacga tgaccagcgt caggccggtc gcgagcagcg cccggaaatc
     7621 gtcggtcgca gcggcagcaa tgcggaaacc gcgctcggcg atgatgagat acaggcccag
     7681 gatcgccagg gcgccgagca tgcccagctc ctcggcgagc gcggcaaaca cgaagtcggt
     7741 gtggatcgcc gggatggacg gcacgtcgcc cactgagggc aacccggcgc cgagcccggt
     7801 gcccaggatg ccgccgcggc cgtacgcgta catggcgcgg atcacctggt agcccgcgcc
     7861 ctgggcatcg gcgaagggat cgatccagat gttgatccgg tcctgcacgt gcgtgaacag
     7921 cacgtacagc agtgccccac ctgccaggaa cagcgcgatg ccgaacagca cgtaggcgat
     7981 ccgccgcgtc gccacgtaga gcagccccag gaacaccagg aagtagagca acgctgcacc
     8041 caggtcgcgc tggacgatga cgatcgccag ggcgaggccc cacatggcga gcatcggcag
     8101 cagatagggc agcggcggca ggttgaacgc gcccacgcgc gtgctcgaca tggcgagcag
     8161 cgcccggttc tcggccaggt aggcggcgag gaacacgacg aggatgacct tgagcagctc
     8221 ggacggctgg ccggcaaacg ggccgatgtg cagcgtcagg cgtgccccac ccacgatgct
     8281 cccgaacacg aacacgagca gcaggagcgc gatcccggcc gccgcccagg tgtacttgta
     8341 gcggcgcagc cagccgtcgc tacgcaccac cacggccgtc acgccgagca gcgtgaaacc
     8401 gagcaggatc cagaccagct gcaacttgcc caggggcagc tcgagaccgg cgaccgactg
     8461 gacgacgagg tcctgcggca gtcggttcat gatcagcagc gagagcccgg cgaggagcgc
     8521 cgccgcgggg aagaggatct cgtccatgcg ccggccggtg atcacgaatg cgaggtggat
     8581 caagccgacg gccacgaggt agatcgccag caacgacatg tcacctggcc cgatcgcgcc
     8641 gctgcggaac gactcgagac tggtccagcc gatcacgagc gccagaccca ccaccaccag
     8701 caggcgcagc tccagccagc gcgggcgggg ccggataccc caccttgtca gagccgctgt
     8761 cattcctggc gcgggcgctc cagccgcatg cgcacttgac cgatctgcac ctcgtcgccg
     8821 aagccaagcg gcgcgaggcc ttcgatgcgc tggccgttga gccatgtccc attggtgctg
     8881 ccgcggtcct cgacgtacca ggcgcggccg cggaacgtga gcagcgcgtg ctcggctgag
     8941 acgaacgagt cgtcgagcac gatcgagttg ttgacgtcgc ggccgagcga gttcaccgcg
     9001 tcgagggaaa tcgacgtgcc cgccggcggc cggccctccg gcgacccgac gaccagcagc
     9061 cgcgcgagcg gccggccggc tccggccacg cccgcgcgca ggtcgcgcca cagggcacgc
     9121 gcgacaaaga agaagaagat gtagatgacc aggacgaagc ccagtcgcag ggcccacagg
     9181 ctcagagtga agacgtccat ccgggaccga ttctgcctcc gcagtagcgc tcacgcgcac
     9241 tgctgcgcgt cgtcggtgac cgcgctagtt gagggcggcc acgtcctcgc gcgaagtcta
     9301 aggcgctggc tcgacggtga ccgtcgagct gccgatctgg agcacgtcac ccggcccgag
     9361 cgcaatctcg gtcactaacg cgccgttgag gtacgagccg ttggtgctgc cgaggtccgt
     9421 gtagacgagc atgcccaggc ggatgccgat ctggccgtgg tggcgtgaca ccttgtcgtc
     9481 ggccaggacg atctcgttgt ccagcgcgcg gcccagacgg atcgtgcctg ggcggacgtt
     9541 gagccgcgac acgggctggc cgggcgagcg cacggcgagc tgggcgcgcg gcgtgttagg
     9601 tcgaggagct gcgaacacgg cggtcgcgcc cggcacggtg gcggcgtcgt cgtcgacatc
     9661 cggcgcatcg tcgccgtcgg cgttgaccgg tacgggagcg ggctccggcg gggcggtcgg
     9721 tgcgttgcgc gtgggagcgg gcgcgcggtc ggcatacaca cgcacgtcac cgctcgcgac
     9781 ggcgttcgag ccgatcacct ccacgcttgg ccgcgcctgg agggtgtatt gatgcgcacg
     9841 ggcatagacc cgcacctgct ccgccaggtc gcgcgtcagg ctggccatgt caccgtcaag
     9901 ggcggccgcg tcggcggtgc tgagcagcac gcgatagtgc gccggcacga acgcccgccg
     9961 ctcacgcacg acgcgctcgg cttccatggc ccgctcgatg ccgcgctgaa tctggacgcg
    10021 ctcgacacgc tcctggaaaa gcctggccgc agggcgctcg aacaggcgct cgaagaaccg
    10081 ctcgacggcg gtcaaagggc ctgccatcag gcgccgagtc taccagcggc cctgagcgcg
    10141 gccggttggg accatctata ggcgcaggcg gtactgatcg ttgagtaccg gtgcagtcgt
    10201 ccgtgcgcga tcctcacgta gatgcagggg gtggacgtct tctgtgagcg ctgcggcagg
    10261 ccgcagccca agcccgaggt ggccgcgccg cgctcggctg gtttgcttgc gcgtcgcgtg
    10321 ctggacgccg tcggcatcac tgagaaccag ccagcaccca cggaggactg gctgcgcctc
    10381 tgcttcaact gccgcggcta ctcgtgcccg gcctgctgga acgagaccgc aggtgtgtgc
    10441 cagacgtgcg agcccctgcc cgagccgatc gtcgaggtgg ccccggagcc gctgcccgag
    10501 ccggtggtca tcgaggcgga gccggtcgtg ccggagccgc tggtcgctgt gccggaaccg
    10561 ttggtcgctg cgccggaacc catggtcgct acgccggaac ccatggttgc ggaaacggtg
    10621 cccgaggtcg aagccgagcc gctgccggtc ttgttcgccg aggcggagct tgagcctgag
    10681 atcgagctcg cgctcgagcg cgagccggtg gaggtcctcg acgcggaccc agtgttcgca
    10741 gtcgaagccg agtcagagtc cgagcccgaa ccagaacggg tagctgccgc cgagcccgaa
    10801 ccagaaccgg tagctgctgc tgagcctgag ccggagccgg agcccgtgga gcggccgcgc
    10861 atgccggtcc tgccgctgcc gcgaccccgc cccgccgacg cgcccgcgat cccactgccg
    10921 ccgctgcccg cctatccagt cgcgccccag atcgcgttca acccgcaggt cagcgtcgcc
    10981 gcgtcctccc agacgagcgc acccgcctat gaggcccatt tgctgcttgc cccgccgccg
    11041 cagctcgcgc cgaacgcgat cgccgcgggc ctgcgcccgt gcccgaactg ccacctgacg
    11101 ctatccgcgc gggcgcactt ctgccgccgc tgcggcgcgg cccaggcgca ctaggcggcg
    11161 gtcgtccccg cgtattgctc gcggtcgcgc ctgacgcgcg cccacgccgc gccgaaaagc
    11221 aggatctggt agctgaagtt cagccacacc agcgcgccga agaccgtggc gatcacgcca
    11281 aagccggcca tgcccccgat gagcagcggc gccagcaggc tgaacaggtt ggtcagcagg
    11341 ccgatgccga agccggccgc gaccgccggc cagaaagcgg cgcgcaggct cggcggcgcg
    11401 gttggcacga cgagatacac cacgagcgcg atcaggacca tgacgacgag cgacaggagc
    11461 ggcgctgcat agcgccagac ggacaactcg cccaccagtt cgtcgagcgt cgcccacaga
    11521 ccgcccagcg agacggtgcc gacgatgaca agcagcagga tcacgatcgt ggcgaagcca
    11581 cggatgcggc gtgagaactc gttgcgcagt ccgccgccgc tgaagatgcg gcgcatcacc
    11641 tcgtcgagca cgccgtagaa ggcactcgcg ccccacagca ggccgatcag gccgatgatc
    11701 gacagtgcgc cgcgaccggc caccgcgccc tcgagtgacg agcggaagaa gtcggccagc
    11761 ggcggcacgt agctgatcaa ctggtcgagc agcgaggcac gctgcagtgg atcttcaatg
    11821 agccagccga tcacgcccga cacgagcagt accaagggga tgaaggcgaa catcgtgccg
    11881 aaagccagcg cggccgcgaa gagagtcgcg ccggtgtcgt tggccgtgtt gatcacgtcc
    11941 aggaccgggc ggacgcggcg atggcccgtg atccccgtga ccagcgcgtc gacgcgcgcc
    12001 cgcagtcggt agatcaaacc tggccctgac gcaggcgcag gcggaggctc agggcttgga
    12061 atcggagctg gctgtgtctg tggctgtgtc tggctttgcg gattggccat cggtggctgt
    12121 gctcggcttc gcctctgtgt cggtggccgg cttgtccctg ggtgcgggtt tgctcgagcg
    12181 atccttcttg gcccagcccg agcccttgaa cactatcgcc ggcgttgaga gcagtttgcg
    12241 catctcggcg ccacagcgct cgcacttggc cggcccggag tcgttaactc catgcatcac
    12301 ttcgacgatg tggccgttgt cgcagatgta gtcgtacagc ggcatgtctc taggcccgcg
    12361 ggcgcatgcc gcacttggga caatactggg cgcgccgatc gatggccggg aagccacagt
    12421 gcgagcacgg ctcgtggcgc gcgccggaga cagccacggg ccgcgcgacg gccccgagct
    12481 cggttgcgtc gtgactgacc aggagattgc ccataagccg ggccagcaac cgctgggcga
    12541 tgcgctgcgt gtcgccgcgg atgccctcgc cgccccagcc gagcaggaag cgcacgacca
    12601 ggtcgccgct cgattcatcc cagtagctca cggggccgcc aaagatcgcg tcgtaacgtg
    12661 gaaagccctc gccgtcgagc tcgccatgga gcagcgcgag cgcgaacgtc tcgtaggcaa
    12721 gatcgaccac gtcgagcgac tcgaacacga agagtgcctg gcgaccgagc ggtgatcggc
    12781 cgtggtgggc gttgatccat tgcagcgcgg cctctggtcg gacgagcgac ggcgcggcat
    12841 cttcgaagtc gatcggcagg gggtcgatca gctccacggg cgagatggcg aatccgctga
    12901 gcagggccgg cagacgctgc ggcacatcgt gggtgcccgg cgtgcgcacg ccgtgactgc
    12961 tcatggagta gtactggtat ttgattgact gcgaggtcac gtcgatctgg tcgggcggga
    13021 tgcgcaccgg ctcgggtgca gctcgtaccg gcttcttctt acggccgatg aacggtattg
    13081 gcatttctag acctcccctc ggcgagcagt cagcgccgcc acctgtgctt ccagctcggc
    13141 ggcgcgtctc cgagcctcat ttaccaccgc cgtgggtgcc ctccctatga agttcgcatc
    13201 agacagccgc gcgcgcgcag catccagctg agcccggagt tcgcgcagct ccttgtcgag
    13261 gcgcgcccgt tcgcgctcga gctgcgttgc ggactgcgtc agccgcgctt cagcgacagt
    13321 gcttacgacg gccacgccct ctgctagcgc ggcgcgatca tcgatgagcg tcgcggttac
    13381 gcgggcgagg cgcgcaacgg ccgcggccat gtccccgtat gcgtcgcgcg cgggaccatt
    13441 cgccagccag atctgcgcgg gcaggatgtc gcccgcctcg atccccgatt cggcacgcgc
    13501 tgagcgcatg gccgtgacca gctcgatgag ccccgccgcg ccgctcgccc gcgccgggtc
    13561 agcggtcgcg gccgcatccg cggcgaccgg ccacggggcg acgatgagca gctccggatc
    13621 gtcggcgcgg tggggcagct tgccccagat ctcctccgtg agcatgggca tgagcgggtg
    13681 cagcaggcgc aggtagcggt cgagcaccca ggtgagcgtc gaccagatcg cgcgcttgcg
    13741 ctccgcgctc gcggacgcgt cgcccaggcc gatcttggcc agctccacgt accagtcgca
    13801 gaaatcgttc cagatcgcgt cgtagagcag ccgcgcgacc tcgccgaact cgaagttcgc
    13861 gtatgccgcc tccgccgcgg ccaatgtgga ctcgcaacgt tgcacgatcc agtgctcggc
    13921 cggaccgagt tggtcggtgt cgatcaggcc cagcgagaag tcgggcggca tctcgttggg
    13981 tcgggcgctc aacacaaagc gcgccgcgtt ccagatcttg ttggcgaagt tgcgcgcgtc
    14041 ctcgagccgc ggccgcgaca tcttctgatc ctggctgggc tcagggccgt ggagcagcgc
    14101 gaagcgcagc gcgtcggcgc ccagctcgtt gatcacgccc agcggatcaa ggacattgcc
    14161 cttcgacttg gacatcttct gccccagcgg gtcgcgcacg atcccgtgca ggtatacgta
    14221 ccggaacggc tcgctgccgg tcagccactc accgagcatc atcatccgcg ccacccagaa
    14281 gaaaaggatg tcgtagccgg tttccatgac ggtggtcggg tagtacttcg ccagatcggc
    14341 agtctcctcg ggccagccca gagtcgacca cggccagagg ccgctggaga accacgtgtc
    14401 gaagatgtcc tcttcctggc gcagctccgc cgcgggccgg ccgcacaggt cgcacccgcc
    14461 tggtccgtcg gccgcgtcag agaccgtgat gtggtcatcg gggcaatacc acgccgggat
    14521 gcgatggccc caccacagct ggcggctcac gttccagtcg tggatcttct ccatccagtc
    14581 gaagaacacc ttgcggtggt gctcctggac gaatgttgtc cggccctcgt tgaccgcggc
    14641 catcgccttg tcggccatgg gtcgcgtgcg cacgaaccac tgtgtcttga gccgcggctc
    14701 gatgacatcg ttgctgcgct cgcagcggcc cagcaccatg tcgtgcggct gctcgccggc
    14761 cagatcgccg ctctcacgca gctcgtcgag gatgcgtgtc cgcgcgtcgt tgagcgtcag
    14821 cccggcgaag gacgcaccgt tctcgttgat gtggccgtcg tcggtcatca cgtcgatgat
    14881 cggcaggccg tggcgcttac cggtctcgaa gtcgtcctgg tcatgggcgg gcgtgatctt
    14941 gac
//
LOCUS       DATBRX010000087         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_698122, whole genome shotgun sequence.
ACCESSION   DATBRX010000087 DATBRX010000000
VERSION     DATBRX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698122"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1055)
                     /locus_tag="VIF08_03215"
     CDS             complement(12..1055)
                     /locus_tag="VIF08_03215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8135025.1"
                     /translation="MTDLSRWGVFLPSYIWAGDGPDRARGIKEFSRAVEDLGFDSLFV
                     TDHLIAAKRFYSVSFLEPLTALAVAAGATSRVRLGTSILIMPLRHPVMLAKELATLQF
                     LSENRVILGAGVGWNEFEYAALGVRKADRGKRTDEMLDIMIPLLEGETVTYRGEFYSV
                     DDLSIEPRSAQRPEIWIGGGSQLADPKSPDLPKFVESVKARVLRTDGWIPRPTCPVPE
                     IANDWRQLQDYYRVHGRDPSECVVAHENFVHVVLSNDPVKVREEQHRAFLNVMSNERG
                     PDYLETVYLFGTPTEIVASLQARVDAGVEYFMLHTMTPDPRQLETWISEIIANVKFPP
                     TAGPVRRMAVAAA"
     gene            complement(1073..>1794)
                     /locus_tag="VIF08_03220"
     CDS             complement(1073..>1794)
                     /locus_tag="VIF08_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8135026.1"
                     /translation="PMNLMSDEMLAAFEAALASLEAAAPGDVRAVVVSSAVERAFSAG
                     SDVKSFAKHAGAAGRAHHTRDEEVCARLAQLPMPTVAAIEANALGGGLEIALACDIRV
                     ASASAKLGLPEVRLGVTPGAGGTQRLPRAVGVARAKELALTGRIIDAHEAERIGLVSR
                     VVPAGQAVAAATEIANEIAQRGPLAVREVKRLIDLSSEADLDTGLAAEIEASVRVFSS
                     ADMLEGMNAFTEKRQPSYKGE"
BASE COUNT          284 a          626 c          591 g          293 t
ORIGIN      
        1 gacacgcagc gtcatgccgc ggcgacggcc atcctgcgca ccggcccggc cgtaggcggg
       61 aacttcacgt tggcgatgat ctcgctgatc cacgtctcga gctgacgcgg gtcgggcgtc
      121 atggtgtgga gcatgaagta ctcgacgccc gcgtcgacgc gcgcctgcag cgacgcgacg
      181 atctctgtgg gcgtgccgaa gaggtacacc gtctcgaggt agtcagggcc gcgttcattg
      241 ctcattacat tgaggaaggc gcggtgctgc tcttcacgca ccttgaccgg gtcgttcgac
      301 agcacgacgt gcacgaagtt ctcgtgggca acaacgcact cgctcgggtc gcggccgtgc
      361 acgcggtagt agtcctggag ctgacgccag tcgttggcga tttccggcac gggacacgtc
      421 ggccgcggga tccagccgtc agtgcgcagg acgcgcgcct tgaccgactc gacgaacttg
      481 ggcaggtcgg gcgacttggg atcggccagt tgcgagccgc ccccgatcca gatctccggc
      541 cgctgggcgg agcggggctc aatcgacagg tcgtcgaccg agtagaactc gccgcgatag
      601 gtcaccgtct cgccttcgag cagcgggatc atgatgtcaa gcatctcatc ggtgcgcttg
      661 cccctgtcgg ctttacgcac gcccagcgcg gcatattcga actcgttcca gcccacgccc
      721 gcacccagga tgacgcggtt ctcgctcaag aactggagcg tggccaactc cttggcgagc
      781 atcaccgggt ggcgcagcgg catgatcagg atcgacgtcc cgagtcggac gcggctcgtc
      841 gcacctgccg ccactgcgag cgcggtgagc ggctccagga acgagacgga atagaaccgc
      901 ttggccgcga tcaggtgatc ggtcacgaag agcgagtcga agccgagatc ctcgacggcg
      961 cgcgagaact ccttgatgcc gcgcgcccga tcggggccgt cgcccgccca gatgtacgac
     1021 ggcaggaata cgccccatcg gctcaggtcg gtcattggct tgccctctcc cctcactcgc
     1081 ccttgtatga cggttgtcgt ttctcggtga aagcattcat gccctcgagc atgtccgcgc
     1141 tggagaagac gcgtactgat gcctcgatct ccgccgcgag accggtgtcc aggtccgcct
     1201 cagacgacag gtcgatcagg cgcttcacct cgcgcaccgc aagcggaccg cgctgtgcga
     1261 tctcgttcgc gatttctgtc gccgcggcga cggcctggcc cgcgggcacg acgcgcgata
     1321 cgaggccgat tcgctccgcc tcgtgggcat caatgatccg gccggtcagc gccagctcct
     1381 tcgcgcgcgc cacgcccacg gcgcgcggca accgttgcgt gccgccggcg cccggcgtga
     1441 cccccagccg cacctcgggc aggcccagct tggcgctcgc cgacgcaact cgtatgtcgc
     1501 acgccagcgc gatctcgagg ccgccgccca gcgcgtttgc ctcgatcgcg gcgaccgtcg
     1561 gcatgggcag ctgcgcgagg cgggcgcaga cctcctcgtc gcgcgtgtga tgcgcccggc
     1621 cggcggcgcc agcgtgtttc gcgaatgact tgacgtccga tccggccgag aaagcccgct
     1681 caacggcact gctgacgacg accgcgcgga cgtcacccgg cgccgccgct tcgagcgacg
     1741 ccagcgcggc ctcgaacgcc gcgagcatct cgtcgctcat gagattcatc ggcg
//
LOCUS       DATBRX010000088        12821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1034145, whole genome shotgun sequence.
ACCESSION   DATBRX010000088 DATBRX010000000
VERSION     DATBRX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12821
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034145"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VIF08_03225"
     CDS             complement(<1..547)
                     /locus_tag="VIF08_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8135027.1"
                     /translation="MIRAVVGWLIAHPRLTALLGAISISFSGILYRVSETTPETAAFF
                     RCLYALPILLFAAWFERRELGALPRRSLLLAAGAGVLFAVDLIFWHHAVDAVGAGLAT
                     VLGNLQVVIVGVGAWLLFGERPSRRELIALPIVLAGVVLISGVLTADSYGANPPLGAV
                     LGIVAAFAYAFYLMVIRRVGRR"
     gene            complement(544..843)
                     /locus_tag="VIF08_03230"
     CDS             complement(544..843)
                     /locus_tag="VIF08_03230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135028.1"
                     /translation="MVEITNNPAEKRWEARLDGELAGYAEYGASPSRVVFTHTVVEPQ
                     FEGRGIGTALAKTALDAAVAAGRRIVPYCPFISAYLRRHHEYDASVDWPTTRTEA"
     gene            881..1252
                     /locus_tag="VIF08_03235"
     CDS             881..1252
                     /locus_tag="VIF08_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY8135029.1"
                     /translation="MSEQDHEGPAVTHAKSSRDAQDAFRAVVPDDARVSVRPMFGSVA
                     AFANDQMFMCLFADELYYRLGEEDRTGLEAAGGRALEPMPGRPMREYVGLAEWRSRTV
                     PSREWAERALAYAISLGPKKK"
     gene            complement(1374..1649)
                     /locus_tag="VIF08_03240"
     CDS             complement(1374..1649)
                     /locus_tag="VIF08_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY8135030.1"
                     /translation="MPDFNGIGWLGWIIVGFFAGALSGVIVRDRTAGGCLANILIGIL
                     GGIVGGLLARQFFNTNEVFGWIGAFLVAFVGAVIVRWLLALANPPRR"
     gene            1768..2862
                     /gene="ugpC"
                     /locus_tag="VIF08_03245"
     CDS             1768..2862
                     /gene="ugpC"
                     /locus_tag="VIF08_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEY8135031.1"
                     /translation="MATVTFDHVFKRYGEVTAVNDLTLDIQDEEFLVLVGPSGCGKTT
                     ALRMIAGLEDVSDGTLKIGDRVVNGLAPKDRDVAMVFQSYALYPHMSVYDNLAFGLKL
                     RKVPKAEIDKRVREAAATIELSNLLDRKPKQLSGGQRQRVALGRAIVREPAVFLMDEP
                     LSNLDAKLRVQTRAEIARLHQRLRTTVVYVTHDQVEAMTMGDRIAVMNFGVLQQVGPP
                     QELYQNPVNKFVAGFIGSPAMNFLSVAPKSDGGTLKLVGDGVELPVPDSLRNVASGGA
                     IEITAGIRPEHFQIMNGAAPGAGAHVRATCDVVEFLGNEELLHVRVGGHDLVAVVGAE
                     HRVRPGDVVELFVPIDKIKLFDTADGKALH"
     gene            complement(2890..4761)
                     /locus_tag="VIF08_03250"
     CDS             complement(2890..4761)
                     /locus_tag="VIF08_03250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8135032.1"
                     /translation="MADATQQELAAVLEELCQAVAADGGAALYLDDGDGALQRVASTG
                     ACGRRPSRLLDRFRDESHDKHSLVLSVPGSAPGVVVLSRTTGGTFTQQDRAVARLYVR
                     RLSEGAGAAGAPLARSVWTHRLEAIQRMGARLSKLGSLDEVAATICAGTFDVIGHDEA
                     HLLLTDEKGFLQAAARATADGRAPRMLPADGEIARHIGRALVSATPVLVQRLKDAGPG
                     RMAPHSLLIAPLLHESHVTGLLVLLSAGEARFDDDHLQLLQILADQAAVALENARLLF
                     GRDELVQELAGLLDISEAAGAASDEGELATLLATRMRSAIDLDGARVSRWDEGSTYLR
                     LVGRDGRDTEQEVVELADAPQRWFALRDGRPAVLQVDALDDTPESRDLRARGVKTLIL
                     MPLTTGGRTIGLVELVALHQPRALGESQLNALEAMARLTATGLERVRLFEQLRNAADT
                     DLVTGVHNHRYLQERLRQEVARSARSHSPLAVLMMDLDKFKQVNDRHGHADGDRVLHN
                     IGATIMAQVRTTDIVARYGGDEFVVLMPDTSAERAELVTRRVVDAILERRHELSNGSH
                     VSVGVSAGLAQYPDDGRTTAQLLATADAAMYEAKRGGGRVIERFAAEPLLEAVARAG"
     gene            complement(4754..5335)
                     /locus_tag="VIF08_03255"
     CDS             complement(4754..5335)
                     /locus_tag="VIF08_03255"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HEY8135033.1"
                     /translation="MAGLLTGGKLAALGSAHIRLWPVALIGLFAQLLLFSSPLAVAVG
                     PWGPSLYVVSTVAVLMALVVNLRQPGFWLIIVGALANFAVIVLNGGQMPASPEAWAAL
                     TGVAAVPTTDFSNSALAGPDTVFAILGDNFVLPPPIPFANVFSIGDVLIGAGGGLFVY
                     QQMHGRVLPRLLRAEPRVRPAGRVSPRVPATHG"
     gene            5684..6277
                     /locus_tag="VIF08_03260"
     CDS             5684..6277
                     /locus_tag="VIF08_03260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8135034.1"
                     /translation="MRIERESDLVEKLVDRQVVRESNYMQVVLDTIETSDGSRRQRDI
                     VLHPGAVAVLAIRPDGRILLVRQYRHAAGSVLLELPAGVLDKLDDGSIEDPAVAGPRE
                     LHEETGYTAKTWRRLGGFWTAPGFAREHMTLLLATDLMADPNHRGPQPDERLIVEAYT
                     LPELLARAGGDEIHDAKTLIGILWLDALVRSGEVKLP"
     gene            complement(6294..7268)
                     /locus_tag="VIF08_03265"
     CDS             complement(6294..7268)
                     /locus_tag="VIF08_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135035.1"
                     /translation="MNNRSAQLLAATLAFLLIVLVGAAIFILLSRPAVPGPTPTPSGS
                     PGFTPTFGPGASGTLLPTASASASPTPIILPTDSPSPTVQPTITPTPSPSPTPSPSPS
                     PSPTLPPPTSEDRHLTIVGFGLDRRTDVGSVQRTVTFFVDGPSLISARLSGVNDGKVR
                     MCLLRNEQDPQRECITAHGGTLSRAVFDVGRTAWNVTLIGAAETLGQYGTLDLTFNAL
                     NPELRLDSFRFNGTGDPRNNGFEIVLGPNLLAGDFHLHAEFSDGSPHPWHLRVAPDDA
                     AGFDQTGGPSTFVDVTTALESGTVYSVLFENPEASASVLLDNVSLTWP"
     gene            complement(7265..7669)
                     /locus_tag="VIF08_03270"
     CDS             complement(7265..7669)
                     /locus_tag="VIF08_03270"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8135036.1"
                     /translation="MTPDDAERLEELAERETAYGLFQRAQALQRKRHNAQAAVLLERA
                     DRLEPRKGSIVEALARACYNSGQHERAAETFRELLDIDPSSHYGHFGLGQSLKQLGQT
                     AEALVHLRLAVALAPESTLYRNALARLTRKSE"
     gene            complement(7680..8732)
                     /locus_tag="VIF08_03275"
     CDS             complement(7680..8732)
                     /locus_tag="VIF08_03275"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY8135037.1"
                     /translation="MTSPKPIMPGIRRTPSRDILSGESADVYFARAEEILSKEGMDPV
                     VAMEVFCRRDAILCGIDEVKVLLAHVFSANGDGVVVESLSDGDRVAPKEVVLRIRARY
                     RTFGLYETAMLGMLAQSTGWATAARECVDAAAPEPIVSFGARHVHPDVTDNLDYAAIV
                     GGCVGASTPAGARLAGLNPTGTMPHSMVLIFGDTVRAAEAFDRHMPPDVPRIILVDTF
                     RDEAEESLRVAEALGDRLYGIRLDTPSERGRVTPDLVREVRARLDQAGHGHVKIIVSG
                     GFDPTRIAFFKEAKAPVDSFAVGSYISGARPIDFTGDLKEIDGQPIAKRGRIPGVTPS
                     PRLVKLDLSAWRATES"
     gene            8807..12202
                     /locus_tag="VIF08_03280"
     CDS             8807..12202
                     /locus_tag="VIF08_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8135038.1"
                     /translation="MEAAMDAAQDVERRIITVLFADLVGFTTLSEQFDAEDVAAIQDR
                     YFGTVRETIGRYGGTLEKFIGDAAMAVFGVPRSRDDDAARAVRAGLALTSAIEQIGAE
                     LGLEPDALRLRVGVNTGEAVIAAGGADSGRVTGDTVNTAARLQTAAPPGRVLIGEKTA
                     LAVADIAELEPAAPFELKGKAEPVAARLVSGWRQEPSHDEAMGGLRAPTLGRAAELAQ
                     LEAAYERAAAGGVERWLIAAPPGVGKSRLLREFAARLESRPGPPLVLRTRSRADTVAP
                     FAAIAILMCEALAESDDLAARRTLIDTLDAAGVPELRRGIVVEACVAVAWPDPSSAAV
                     HPQEEREALFGAWLEGIDALAAGRTQVWLVEDVHWAGGDVLAFLDRAVARGAAGPPGG
                     RLIVATTRPSLLEANPDWSAELAENGRQLLQLSTLAPTDARSLVAALVGDALPDPLVE
                     RIAERSDGNPLFIEELLRTWVSVGTLVREDERWRLTVGADDIPLPTSVQSIYAAQLDD
                     LPLDARNLARRASVAGRRFPVGALQPLGAAAEGGLGPLQRRQLVVGPVDEPVVGTAFS
                     YRHALLRDAGYASLARAERARLHIRLARWLEGAAGERATELAEQMAGHYAAALESAPA
                     LTREVEGLEREALRRLAADWYERAGQGTLALSAHDAARQLFKRSIELTPDGDDLARAR
                     RWEKLGDATAFAADMDEGAAAYEKAIDLYRAAAGAAARATEAAARLAEARSGLARSTA
                     ALSDVRYQQLRFNDARDIANEALAEVGDEDRAAIARLLTARALGSLGASGPSPDIERD
                     LERATELARGSAEPRVELKARSGLTMLRSESGRGTMADWQELSEMAQATGDWREAVGA
                     STSAIMYLLDDRAVESFAPIERTRETAASHGLTEETGWLDYLAAEAAFVSGDWARARE
                     VGGRAIEVGQANAYLRMTVRTWHVLIPIAGATGDRATLDEAARWYRSLEGKFEFPDSP
                     YSRVIRVAQDLEMAAAGLWPELVPEVEPRIASYNDDPSGPSWSAALDRVFRAWLEAGD
                     LDGAARAFAALDEAVARMGSVTNLGHGAHRLMAGRLALARGDRDAAASAGRAALEHFR
                     ASNAPWWMAKAMRLVERSGAADDEMLGQVREIEHKLGSAGPTA"
     gene            complement(12210..12719)
                     /locus_tag="VIF08_03285"
     CDS             complement(12210..12719)
                     /locus_tag="VIF08_03285"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY8135039.1"
                     /translation="MAAHVGVKVDGWLAELGLEPVERAEREGATSWDLVLDGRRRRGV
                     RVTLIFDPAVALVAWVHFGPPLSDNFRKTYRQLLRWNDELPFAKFALSEDERPILTAE
                     VPADGLTRDVVGTLLARVLAICDLVHEPVLALVGELRRKDTALAATEREPAGVTLLER
                     YAAELSELT"
BASE COUNT         2079 a         4320 c         4584 g         1838 t
ORIGIN      
        1 ggcgccggcc gacacggcgg atgaccatca ggtagaacgc gtacgcgaac gcggcgacga
       61 tgcccagcac cgcgcccagc ggtgggttcg cgccgtagct gtccgctgtc agcacgcccg
      121 agatgagcac cacgccggcg agcacgatcg gcagcgcgat cagctcgcgc ctcgagggcc
      181 gctccccgaa gaggagccat gcgcccacgc cgacgatgac cacctggagg ttgcccagca
      241 cggtcgcgag gccggccccg accgcgtcga ccgcgtggtg ccagaagatg aggtcgaccg
      301 cgaatagcac gcccgcgcca gccgcgagga gcaggctgcg ccggggcagc gccccgagct
      361 cacgacgctc gaaccaggcc gcgaagagca ggatcggcag cgcatagagg caacggaaga
      421 acgccgcggt ctcgggcgtc gtctccgaga cgcgatacag gatgccggag aacgagatcg
      481 agatcgcacc caggagagcg gtgagccgcg gatgggcgat gagccagccg acgaccgcgc
      541 gaatcacgcc tccgtccgcg tcgtgggcca atcgacggac gcgtcgtact cgtggtggcg
      601 acgcaggtac gcgctgatga atgggcagta cggcacgatc cgccgacccg ccgcgaccgc
      661 cgcatcgagc gcggtcttgg ccagggccgt gccgatgccg cgaccctcga actgcggctc
      721 gacgacggtg tgcgtgaaca cgacccggct cggcgacgcg ccgtactcgg cgtagcccgc
      781 cagctcgccg tcgagccgcg cctcccaccg tttctccgcc ggattgttcg tgatctcgac
      841 cacgcacaga ggttatcggc gcgctgcgat acttgagtcg atgtcagaac aagatcacga
      901 ggggccagcg gtcacgcatg ccaagagcag ccgcgacgca caggacgcgt ttcgcgcggt
      961 agtgcccgac gacgcgcggg tcagcgtgcg gccgatgttc ggcagcgtgg cggctttcgc
     1021 gaacgaccag atgttcatgt gcctgttcgc cgacgagctc tattaccgct tgggcgagga
     1081 ggatcggacg gggctggagg cggctggtgg tcgggccctc gagcccatgc ccggccggcc
     1141 catgcgcgag tacgtgggtc tggccgaatg gcgctcacga accgtgccgt cgcgcgagtg
     1201 ggcggagcgc gccctggctt acgcgatcag cctggggccc aagaagaagt aacgcgaccc
     1261 ttctgggggc gactaccgcc gaggcggccg caaggaggca gcgaccacac tccaaagagt
     1321 ggggtcgcgg acgacgcgca gcagtgcgcg tgagcgctac tgcggaggta gaactaccgc
     1381 cgaggcggat tcgccagcgc cagcagccag cgcacgatca ccgcgccgac aaaggcgacg
     1441 aggaaggcgc cgatccagcc gaaaacctcg ttggtgttga agaactggcg cgccagcagg
     1501 ccgccgacga tgccgcccag gatgccaatc aggatgttgg ccaggcagcc acctgccgtc
     1561 cggtcgcgca cgatcacgcc cgatagggcg ccggcgaaga agcccacgat gatccagccg
     1621 agccagccga tgccattgaa gtcgggcact ggtttttcct ccgtgttgcc tgcgcatcgt
     1681 aattggtcct agcatgcgca caacgagacg gccgagccgc attgcaccga agccggtgcc
     1741 ggcttacggc gtttctggag ggttgtcatg gcgaccgtca ctttcgacca cgtcttcaaa
     1801 cgttacggcg aagtcaccgc cgtcaacgat ctcaccctcg atatccagga cgaggagttc
     1861 ctggtcttgg tcggcccatc tggctgcggc aagacaaccg cgctgcgcat gatcgccggc
     1921 ctcgaggacg tgtccgacgg cacgctcaag atcggtgacc gcgtcgtcaa tggcctggcg
     1981 ccaaaggacc gcgacgtggc gatggtcttc cagagctacg cgctctatcc gcacatgagc
     2041 gtctacgaca acctggcatt cggcctgaag ctgcgcaagg tccccaaggc ggagatcgac
     2101 aagcgcgtgc gcgaggccgc ggcgacgatc gagctttcca acctgctcga ccgcaagccc
     2161 aagcagctgt cgggcggcca gcgccagcgt gtcgcactgg gtcgcgcgat cgtgcgcgag
     2221 ccagccgtgt tcctgatgga cgagccgctg tccaacctcg atgccaagct gcgcgtccag
     2281 acgcgtgccg agatcgcgcg cctgcaccag cgcttgcgca ccaccgtcgt ctacgtgacc
     2341 cacgaccagg tcgaggcgat gaccatgggc gaccggatcg cggtcatgaa cttcggcgtg
     2401 ctccagcagg ttggcccgcc ccaggagctg taccagaacc cggtgaacaa gttcgtcgcc
     2461 ggcttcatag gcagcccggc catgaacttc ctgagcgtcg ctcccaagtc agacggcggc
     2521 acgctcaaac tcgtgggcga tggtgtcgag ctgccggtcc cggacagcct ccggaacgtc
     2581 gccagcggcg gcgcaattga gataaccgcg ggcatacggc ccgagcactt tcagatcatg
     2641 aacggcgccg cgccgggtgc cggcgcgcac gtccgcgcca cgtgcgacgt ggtcgagttc
     2701 ctcggcaatg aggagctgct ccatgtgcgc gtcggcgggc acgacctcgt ggcagtcgtc
     2761 ggtgcggagc atcgcgtccg ccccggtgac gtggtggagc tgttcgtgcc gatcgacaag
     2821 atcaagctct tcgacaccgc ggacggcaag gccctccact agccgcaacc gacccgcggc
     2881 cgttcggggc taacccgcgc gggcgacggc ctcaagaagc ggctcggccg cgaagcgttc
     2941 gatcacgcgg ccgccgccgc gcttggcctc gtacatggcc gcgtcggcgg tcgcgagcag
     3001 ctgcgccgtc gtgcgtccgt cgtcggggta ctgcgccaac ccggcgctga cgccgacact
     3061 cacgtggctg ccgttggaca gctcgtggcg gcgctcgagg atggcatcca cgacacgccg
     3121 cgtgaccagc tcggctcgtt cggcgctcgt gtcgggcatg aggaccacga actcgtcgcc
     3181 gccgtagcgt gccacgatgt cggtggtgcg aacctgggcc atgatcgtgg cgccgatgtt
     3241 gtgcagcacg cggtcaccgt cggcgtggcc gtggcggtcg ttgacctgct tgaacttgtc
     3301 gaggtccatc atcagcaccg ccagcggcga atggctgcgc gcgctgcggg cgacctcctg
     3361 gcgcaaccgc tcctgcaggt agcggtggtt gtgaacgccg gtcacgagat cggtgtcggc
     3421 ggcgttgcgc agctgctcga agaggcgcac gcgctcgagc cccgtggcgg tcaggcgggc
     3481 catcgcctcc agggcgttga gctgcgactc acccagggcg cgcggctgat gcagcgccac
     3541 cagctcgacc aggccaatcg tcctgccacc ggtggtcagc ggcatcagga tgagcgtctt
     3601 cacgccgcgc gcacgcagat cgcgtgactc aggagtgtca tcaagcgcat caacctgcag
     3661 gaccgcgggc cggccatcgc gcagcgcgaa ccagcgctgc ggcgcgtcgg ccagctcgac
     3721 cacttcctgc tcggtgtcgc gaccgtcgcg gcccaccagg cggaggtacg tcgagccctc
     3781 gtcccagcgg ctgacccgcg cgccgtcaag atcaatcgcg ctgcgcatcc gcgtggccag
     3841 cagggtcgcg agctcaccct catcggacgc cgcgcctgcc gcttcgctga tatcgaggag
     3901 gcccgcgagc tcctgcacga gctcgtcgcg gccgaacagc agacgcgcgt tctcgagggc
     3961 gacggcggcc tggtcggcca ggatctgcag cagttgtagg tggtcgtcgt cgaagcgcgc
     4021 ctcaccagcg ctcagcagga cgagcaggcc ggtcacgtgg gactcgtgca gcaacggcgc
     4081 gatgagcagc gagtggggag ccatacggcc gggaccggcg tccttgaggc gctgcacgag
     4141 gaccggcgtt gccgacacca acgcgcgacc gatgtggcgc gcgatctcgc cgtcggcggg
     4201 cagcatccgc ggcgcgcggc cgtcagcggt cgcgcgggcc gcggcctgca ggaagccctt
     4261 ctcatcggtc agcagcaggt gcgcctcgtc gtggccgatg acatcgaacg tgccggcgca
     4321 gatggtcgcg gccacctcat cgagcgagcc cagcttgctg agccgcgcac ccatgcgctg
     4381 gatcgcctcc agccgatggg tccagaccga ccgggcgagc ggcgcaccgg ccgcgcccgc
     4441 gccctctgat agacggcgaa cgtagagtcg tgcgaccgcg cgatcctgct gcgtgaatgt
     4501 gccgcccgtc gtccggctga gcacgacaac gcccggcgcc gagccgggga cgctcagcac
     4561 gaggctgtgc ttgtcgtggc tctcgtcacg gaaccggtcg agcagcctcg acggccgacg
     4621 gccgcaggcc ccggtggatg cgacgcgctg caatgcgccg tcgccgtcat cgagatagag
     4681 cgccgccccg ccatccgctg cgaccgcctg gcagagctcc tcgaggacgg ccgcgagctc
     4741 ctgctgcgta gcgtcagcca tgcgtggctg gcacccgggg cgaaacgcga cccgcaggcc
     4801 ggacgcgcgg ctccgcgcga aggaggcgcg gcagaacgcg gccgtgcatc tgctgataga
     4861 cgaacagtcc tccgccggcg ccaatcagga cgtcgccgat cgagaacacg ttggcgaacg
     4921 gaatgggcgg cggcagtacg aagttgtcgc ccaggatggc gaacacggtg tcgggcccgg
     4981 ccagcgccga gttgctgaag tccgtggtcg gcacggcggc cacaccggtc agcgcggccc
     5041 atgcctctgg cgatgcgggc atctggccgc cgttcaggac gatgaccgcg aagttggcca
     5101 gcgcgcccac gatgatcagc cagaagccgg gctggcggag gttgacgacg agggccatca
     5161 gcaccgccac cgtgctcacc acgtacagcg agggacccca cgggccgacg gcgacggcga
     5221 gcggcgagct gaacagcagc agctgcgcga agaggccgat cagcgccacc ggccatagcc
     5281 ggatatgggc cgagcccagg gcggcgagct tgccgcccgt gagcagccca gccatcaggc
     5341 caaggatcac ggcatacaga aggagcatcg cctatttgcg cccccgcgcg gcctccagat
     5401 aaacggaacg gcgcgtctct cgtacgcgcc gttgcccggt caattagatg aatgctgccc
     5461 gctgggagtg gctagagcca ccaccggaac ggcgcagcga cgacgagcac gaaggccgcc
     5521 agcacgacga ggctgaaggc gatctgccgg acgttggcca ttggtccatc tccagtgcgc
     5581 acgaccccct ggtgggttcg cgaggtcgtc aatttggcag gtcgaagcgg ctatacgcca
     5641 tgagaaggaa gtactgggac gtggactggc atactcctca gcgatgcgaa tcgagcgtga
     5701 atcggacctc gtcgaaaaac tggtcgaccg acaagtggtg cgcgagagca actacatgca
     5761 ggtcgtgctc gacacgatcg agacgtcgga cggcagtcgg cgccagcgcg acatcgtcct
     5821 ccaccccggc gcggtggccg ttctcgccat acgccccgat ggccgcatcc tcctggtacg
     5881 gcagtaccgt cacgcggctg gctcggtgct gctcgagctg ccggccgggg tgctggacaa
     5941 gcttgatgac ggctcgatcg aggacccggc cgtcgccggt ccgcgcgagc tgcacgagga
     6001 gaccggctac acggcgaaga cctggcgcag gctgggtggc ttctggaccg cgccgggatt
     6061 tgcgcgcgag cacatgacgc ttctcctggc gaccgatctg atggcggacc cgaaccaccg
     6121 gggcccacag cccgacgaac ggctgatcgt ggaggcctac acgctgcccg agctgctggc
     6181 gcgcgccggc ggcgacgaga tccacgacgc gaagacgctc atcggcatcc tgtggctcga
     6241 tgcgctggtg cgctccggcg aggtgaagct gccctagcgg gctggcacga ttcctacggc
     6301 caggtcaggc tgacgttgtc gaggagcact gacgcgcttg cctcgggatt ctcgaacagg
     6361 accgaataga cggtcccgct ctcgagggcg gtggtcacgt ccacaaacgt agaaggcccg
     6421 ccggtctggt cgaaacccgc ggcgtcgtcg ggcgccaccc ggaggtgcca cggatgcggg
     6481 ctgccatcgc tgaactcggc atgcaggtgg aagtcaccgg cgaggagatt cggtccaaga
     6541 acgatctcga agccgttgtt acgcggatcc ccggtgccat tgaaccggaa ggagtccagg
     6601 cgcaattcgg gattgagcgc attgaacgtg agatccagcg tgccgtattg gcccagcgtt
     6661 tcggctgcgc cgatgagggt cacgttccat gccgtgcggc ccacgtcgaa caccgcgcgt
     6721 gacagcgtcc cgccatgggc tgttatgcat tcgcgctgcg gatcctgttc gttgcgcagc
     6781 aggcacatcc gtaccttgcc gtcattcacg cccgacaggc gtgcgctgat gaggctcggg
     6841 ccgtccacga agaacgtcac ggtccgctga accgatccga cgtcggtccg gcgatcgagg
     6901 ccaaaaccaa caatggtgag gtgccgatcc tcggaggttg gcggtggcaa cgttggcgac
     6961 ggcgacggac tgggcgaagg ggtcggcgaa ggtgatggcg tgggggtgat cgtcggctgg
     7021 acggtcgggc tggggctgtc ggtcggaaga atgattggtg tcgggctggc cgacgccgag
     7081 gcagtgggca gcagggtccc gctggcgccg gggccgaacg ttggtgtgaa gccaggcgaa
     7141 ccggatggcg tgggtgtcgg tccgggcacc gccggccggc tcagcaggat gaagatcgcc
     7201 gccccgacga gcacgatcag caggaacgcg agcgtcgccg cgagcagctg cgcggagcgg
     7261 ttgttcattc gctcttgcgc gtcagccgcg cgagcgcgtt gcggtagagc gtgctctcgg
     7321 gcgcgagtgc gaccgcgaga cgcagatgga ccagcgcctc agccgtctga cccagctgct
     7381 tgagcgactg gcccaggccg aaatggccgt agtgggacga cggatcgatg tcgagcagct
     7441 cgcggaaggt ctccgcggcg cgttcgtgct ggccgctgtt gtagcaggcc cgggcgagcg
     7501 cctcgacgat cgagcccttg cgcggctcga ggcggtcggc acgctcgaga aggaccgccg
     7561 cttgagcgtt gtggcgctta cgctggagcg cctgggcacg ctggaagagg ccatatgccg
     7621 tttcacgctc ggccagctcc tcgagccgct cggcgtcgtc cggagtcatc acggtgaggt
     7681 taagactcgg tcgcgcgcca cgcagaaagg tccagcttca cgaggcgcgg gctgggcgtg
     7741 acgcccggga tccggccgcg cttggcgatg ggctggccgt cgatctcctt caggtcgccg
     7801 gtgaaatcga tgggccgcgc gccgctgatg tagctgccca cggcgaacga gtcgacgggc
     7861 gccttggcct ccttgaagaa cgcgatgcgc gtggggtcaa agccgccgct gacgatgatc
     7921 ttgacgtgac cgtggcccgc ctggtcgagc cgcgcccgca cctcgcggac caggtctgga
     7981 gtcacgcgac cacgctcact gggcgtatcg aggcgaatgc cgtagagccg atcgcccagc
     8041 gcctccgcga cccgcagtga ctcctccgcc tcgtcgcgga aggtgtcgac gaggatgatg
     8101 cgcggtacgt cgggcggcat gtggcggtcg aaggcctccg ccgcgcgaac cgtgtcgcca
     8161 aagatcagca ccatcgagtg gggcatcgtg ccggtggggt tcaagcccgc cagccgcgca
     8221 cccgcgggcg tcgacgcgcc gacgcagcca ccgacgatcg ccgcgtagtc gaggttgtcc
     8281 gtcacgtccg ggtgaacgtg gcgcgcgcca aacgagacga tcggctccgg cgcggccgcg
     8341 tcaacgcact cgcgcgccgc cgtggcccag ccggtcgact gagcgagcat gccgagcatc
     8401 gcggtctcgt acaggccgaa cgtgcggtac ctggcgcgaa tgcggagaac gacctccttg
     8461 ggtgcaacgc gatcgccgtc ggacagggac tccacgacga ccccgtcgcc gtttgcgctg
     8521 aagacgtgcg cgagcaggac cttgacctcg tcgataccgc acaggatcgc gtcgcggcga
     8581 cagaagacct ccatcgccac gaccgggtcc atgccctcct tggacaggat ttcctcggcg
     8641 cgcgcgaagt aaacgtcggc ggactcgccg ctcaggatgt cgcgcgacgg ggtccgccgt
     8701 atgccgggca tgatgggctt gggtgacgtc attggtcgcc aagtatgaag gcacgctgag
     8761 cgcgcggccg ggcgattgct gccgacagtg ccaataggat ggggcgatgg aggcagcgat
     8821 ggacgccgcg caggacgtcg aacgccggat catcacggtc ctgttcgccg acctggtcgg
     8881 attcacgacg ctgtccgagc aattcgacgc ggaggacgtg gcggccatcc aggaccgcta
     8941 tttcgggacc gtgcgcgaga cgatcgggcg ctacggcggg acgctcgaga agttcatcgg
     9001 cgatgcggcg atggccgtgt tcggcgtgcc gcgttcgcgc gacgacgatg ccgcgcgcgc
     9061 ggtgcgtgcc gggcttgccc tgacttcggc gatcgagcag atcggcgcgg agcttggcct
     9121 ggagcctgat gccctgcggc tgcgcgtcgg cgtgaacacc ggtgaggcgg tcatcgcagc
     9181 gggcggcgcc gacagcggcc gggtcaccgg cgacacggtc aatacggcgg cgcggctgca
     9241 gacggccgct cccccgggcc gggtgctgat cggcgaaaag accgcgctcg cggtcgccga
     9301 catcgcagag ctggagccag ccgcaccgtt cgagctgaaa ggcaaggccg agccggtcgc
     9361 ggcgcgactc gtcagtggct ggcgccagga gccatcgcac gacgaggcca tgggcggtct
     9421 gcgtgcgccc acccttggcc gcgcggcgga gctggcacag ctcgaagcgg catacgagcg
     9481 tgctgccgcg ggcggcgtgg agcgctggct gatagccgcg ccacccggcg tcggcaaatc
     9541 gcgcctgctg cgcgaattcg ccgcgcgact ggagagccgg ccagggccgc cgctggtgct
     9601 acggacgcgc agccgcgccg acaccgttgc acccttcgcc gcgatcgcga tcctgatgtg
     9661 cgaggcgctg gcggagagcg acgatctcgc ggcgcggcgg accttgatcg acaccctcga
     9721 tgcggcgggc gtgcccgagt tgcgccgcgg catcgtggtg gaggcgtgcg tcgccgtggc
     9781 atggcccgac ccgagcagtg ccgccgtgca tccccaggag gagcgcgagg cactctttgg
     9841 cgcctggctc gagggcatcg acgcgctggc cgccggccgc acgcaggtct ggctggtcga
     9901 ggatgttcat tgggccggcg gcgacgtgct ggcattcctc gatcgcgcgg tcgcgcgcgg
     9961 cgcggccggc ccgccgggtg gtcgcttgat cgtggccacg acgcgcccgt cgctgctcga
    10021 ggccaatccc gactggtcgg ccgagctggc cgagaacggc cgacagctgc tccagctgtc
    10081 cacgctcgca cccacggacg ctcgttcact ggtcgccgcg ctcgtgggcg acgcgctgcc
    10141 cgatcccctc gtcgaacgca tcgccgagcg cagcgatggc aacccactct tcatcgagga
    10201 gctgctgcgc acctgggtca gcgtcggcac gcttgtgcgc gaagacgagc gctggcggct
    10261 gaccgttggc gcggacgaca tcccgctgcc gacgagcgtc caatccatct acgccgcgca
    10321 gctggacgac ctccctttgg acgcgcgcaa cctcgcccgc cgcgcgtcag tggccgggcg
    10381 gcggtttccg gtaggcgcgc tgcagcccct gggtgccgca gctgagggcg gcttggggcc
    10441 cctgcagcgg cgacagctcg tcgtcggccc ggtggacgag ccggtggtcg gcaccgcgtt
    10501 ctcataccgg cacgctctgc tgcgcgatgc gggatacgca tcacttgctc gtgccgaaag
    10561 ggcgcgactg catatccggc tggcgcgctg gctggagggt gcggccggtg agcgggccac
    10621 ggagctcgcc gaacagatgg ccggtcacta cgcagccgcg ctcgaatctg ccccagcgct
    10681 cacgcgcgag gtggaaggac tcgagcgtga ggcactgcgc cggctcgctg ccgactggta
    10741 tgagcgcgcc ggccagggca ccctcgcgtt atcggcgcac gatgccgcgc gccagctgtt
    10801 caagcgctcg atcgagctca ctccggacgg agacgacctg gctcgggcgc ggcgctggga
    10861 gaagctgggt gacgcgaccg cgttcgcggc cgacatggat gagggcgccg ccgcgtacga
    10921 gaaggcgatc gacctgtatc gggcggctgc cggggcggcc gcgcgcgcga cggaggcggc
    10981 cgcgcgcctg gcagaggcac gcagcgggct cgcgcgaagc accgccgcgc tctccgacgt
    11041 gcgctaccag cagctgcgct tcaacgacgc gcgcgacata gccaacgagg ccctcgccga
    11101 ggttggcgac gaggaccgcg cggccatcgc gcgtctactc acggcgcgcg cgctgggcag
    11161 cctgggggcg agcggtccgt cgccggatat cgagcgcgac ctcgagcgag ccacggagct
    11221 ggcacgtggg tcggccgagc ccagggtgga gctgaaggcg cgcagcggcc tgacgatgct
    11281 gcgctccgaa tcgggtcggg gcacaatggc cgactggcag gaactgtcgg agatggccca
    11341 ggccacgggc gattggcgtg aagccgtggg cgcatcgacc agcgcgatca tgtacctgct
    11401 tgacgatcgg gcagtggaaa gcttcgcccc aattgagcgg acgcgtgaga cggcagcgtc
    11461 gcacggcctg accgaggaaa ccggctggct ggattacctc gccgcagagg ccgctttcgt
    11521 gagcggtgac tgggcgcgcg cccgcgaggt cggcggcagg gcgatcgagg tcggccaggc
    11581 caacgcctat ctgcggatga ccgtccggac gtggcacgtc ctgatcccta tcgccggcgc
    11641 aaccggtgac cgcgcaacgc tggatgaagc agcccgctgg taccgctcgc tggaaggtaa
    11701 gttcgagttc cctgactcgc cctactcgcg cgtcatccgc gtcgcccagg acctggagat
    11761 ggccgcggcc ggcctctggc cggagctcgt gccggaggtg gagccgcgga tcgcgtccta
    11821 caacgacgac ccttcaggcc cgagctggtc cgcggccctt gaccgcgtct ttcgcgcgtg
    11881 gctcgaggcc ggtgatcttg atggcgcggc gcgcgcattc gcagcgctcg acgaggccgt
    11941 cgcgcggatg ggatcggtga ccaatctcgg ccacggcgcc caccggttga tggccggtcg
    12001 cctggcgctg gcgcgcggcg atcgcgatgc cgctgcctca gccggtcgcg cggcgctcga
    12061 gcacttccgc gccagcaacg caccgtggtg gatggccaag gcgatgcgcc ttgtcgaacg
    12121 gtcgggcgcg gccgacgatg agatgctggg tcaggtacgc gagatcgagc acaagctcgg
    12181 atccgcggga ccgaccgcct gagacgcggc taggtcagct cgctcagctc cgccgcataa
    12241 cgctcgagca gcgtaactcc ggccggttcg cgctccgtcg cggcgagggc tgtgtccttg
    12301 cgccgcagct cgccgaccag cgcgaggacg ggctcgtgca ccaggtcgca gatcgccagc
    12361 actcgcgcga gcagcgtccc gaccacgtct ctcgtcaagc cgtcggccgg gacctcagcg
    12421 gtcaggatgg gacgctcgtc ctcggatagc gcgaacttgg cgaacggcag ctcgtcgttc
    12481 cagcgcagca gctggcggta ggtcttacgg aagttgtcgg acagcggggg gccgaagtgg
    12541 acccacgcga ccagggccac ggcgggatca aagatcagcg tgacgcgaac gccccggcgc
    12601 cgccgcccgt ccagcaccag gtcccatgac gtcgcgccct cgcgctcggc ccgctcaact
    12661 ggctcgaggc cgagctccgc cagccagccg tcgaccttca ccccaacatg cgctgccatc
    12721 cggtgaagta tcttcgacgg catggcgaaa gagctgcgcg ttctgatcga ggagccgccc
    12781 aagggcaagc gctgggtggc gatcgcggcc gactggccgg g
//
LOCUS       DATBRX010000089         4625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_28472, whole genome shotgun sequence.
ACCESSION   DATBRX010000089 DATBRX010000000
VERSION     DATBRX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28472"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VIF08_03290"
     CDS             <1..429
                     /locus_tag="VIF08_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135040.1"
                     /translation="GQLGLGVQDSGPVTVGDAELAHPGRDLLADVLADLVSNNTITQA
                     QSDAITQALADKVDALKADMEARRALFESFIEDGVITQDEIDQLPADDPLRVAFDSIA
                     NDGQVTLDQLRGFGPGFGPGHHGRGPGGFMFGESSPNTDS"
     gene            complement(497..1864)
                     /locus_tag="VIF08_03295"
     CDS             complement(497..1864)
                     /locus_tag="VIF08_03295"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY8135041.1"
                     /translation="MTDTDFETNPERLAQRFPADFTWGFAASAYQIEGAANEDGRGPS
                     IWDTFSHTPGKTANGETGDVACDHYHRYRDDVRLMAELGAHAYRFSVSWPRVMPEGRG
                     TVNQQGLDFYRRLVDELAAAGVEPTLNLFHWDLPQALQDKGGFANPDIVGWFTDFAHL
                     MATRLGDTVTDWMTFNEPAVYAFLGHADGIHAPGLTHWPTAIKVADNELRAHAAAADA
                     IKSEVPAARIGVAVDINHFAPASDSERDVRAAAAYRATRDTWFLDPLFGRGYPALGVE
                     QHRAAGHLEGVELADPPHGRLDYLGVNYYRQETVHAKSDLPFDWEVRPRKGIELTEMD
                     WEVVPDGLTAGLTWLQHEYAPAEIVITENGAAYPDTRAVNGEVQDDDRVSYLARHIAA
                     AADARDAGVNLRGYFVWSLMDNFEWSLGYGKRFGVIRVDFEDQRRTPKRSARWYQRLI
                     AASRR"
     gene            complement(1910..3307)
                     /locus_tag="VIF08_03300"
     CDS             complement(1910..3307)
                     /locus_tag="VIF08_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8135042.1"
                     /translation="MTLRARLVLAMGALLALALIVSGGAVVSLTRANLLADLDHDLLT
                     VSPADVAPGGPQQPDATGRRFAVLLFDREGQRIQTFPSGFSSSPDPLPDVTITGAQAV
                     PIGAVVERPATDGSLTYRVVAVRARLGIPPQVLTVVFAAPTRALEGTIVSLVRTLVVV
                     GLVVLVLLVVIGWLLIRRNLRALERMTATAEQISAGDLSQRVGMPDDGSEVGKLGHAF
                     DAMLDQIETAFASQQQALRAKEASEQMLRQFVADASHELRTPLTTVRGYADLHRAGGL
                     EQAAELDRAMDRIGSESRRMSVLVEDLLLLARLDQGRPPRQDRVSLSALLSDAVGDLR
                     AVDPGRPITASLAADVTVTGDEDRLRQLVGNLLANVRVHTPPDSPLEVSLSSGDQSVV
                     LTVADHGPGIDPAHVEHVFDRFYRADAGRTRDRGGSGLGLAIAASIAAAHGGTLEHSP
                     TPDGGATFTLTLPRP"
     gene            complement(3320..4057)
                     /locus_tag="VIF08_03305"
     CDS             complement(3320..4057)
                     /locus_tag="VIF08_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8135043.1"
                     /translation="MNTSALPVRNDARAERRGRVLVVDDEATITELLSTALRYMGYQV
                     ATAATGNAALESASKSPPDLVVLDVMLPDIDGFEVCRRLRADGDFVPVIFLTARDAEE
                     DRVTGFIRGGDDYVTKPFSLEELTLRIGALLRRTKTAADSPAEHRLKYRDLEMDEDRH
                     QVWRGGTEVRLSPTEFRLLRYLMLNAERVLSKQQILDHVWQYDFNGDDNVVETYVSYL
                     RRKLDTGHEPLIRTVRGFGYTLRAEAS"
BASE COUNT          720 a         1594 c         1584 g          727 t
ORIGIN      
        1 ggccagctcg ggctgggcgt ccaggacagc gggcccgtca ccgtcggcga cgccgagctg
       61 gcccatccgg gccgcgacct gctggccgat gtactggccg atcttgtcag caacaacacg
      121 atcacccaag cgcagtcgga cgccatcacc caggcgctgg cggacaaggt cgatgcgctc
      181 aaggccgaca tggaggcgcg ccgtgcgctg ttcgagagct tcatcgagga cggcgtgatc
      241 acccaggacg agatcgacca gctgcctgct gacgatccac tccgcgtcgc gttcgacagc
      301 atcgccaacg acggccaggt cacgctcgac cagctacgcg gctttggtcc cggcttcggg
      361 ccggggcatc acggtcgcgg cccgggcggc ttcatgttcg gcgagtcctc gcccaacacg
      421 gacagctagt cggcaacccc actcaatcgc cggagggcct cctcacgagg gctctccggc
      481 ttttcgttgt ccggcgtcag cgccggctgg cggcgatcag gcgctgatac cagcgcgcgc
      541 tgcgcttggg ggtgcgccgc tgatcctcga aatcgacgcg gatgacgccg aagcgcttgc
      601 cgtagcccag gctccactcg aagttgtcca tcagcgacca gacaaagtag ccgcgcaggt
      661 tcacgccggc atcccgcgcg tcggcggctg cggcgatgtg gcgcgcgagg tacgacacgc
      721 ggtcgtcgtc ctgcacctcg ccattgacag cgcgcgtgtc cgggtaagcg gcgccgtttt
      781 ctgtgataac gatctcggcc ggcgcgtact cgtgctgcaa ccaggtcaga cctgcggtca
      841 gcccgtcggg tacgacctcc cagtccatct cggtcagctc gatgcccttg cgcggccgca
      901 cctcccagtc gaacggcaag tctgacttcg cgtggaccgt ctcctggcgg taatagttga
      961 cgcccaggta gtcgagccgg ccgtgcggcg gatcggcgag ctcgacgccc tcgaggtgtc
     1021 ccgccgcgcg atgctgctcg acgcccagcg ctgggtagcc gcgaccgaag agggggtcga
     1081 ggaaccacgt gtcgcgcgtc gcgcggtaag ccgccgctgc acgcacgtcg cgctccgaat
     1141 cgctcgccgg cgcgaagtgg ttgatgtcaa ccgcgacgcc aatgcgcgcc gccggcacct
     1201 cgctcttgat cgcgtcggcg gccgcggcgt gggcacggag ctcgttgtcg gcgaccttga
     1261 tcgcggtggg ccagtgggtc aggcccgggg cgtggatacc gtcggcgtgg cccaggaagg
     1321 cgtacaccgc gggctcgttg aaggtcatcc agtcggtgac cgtgtcgccc aggcgcgtcg
     1381 ccatcaggtg cgcgaagtcg gtgaaccagc cgactatgtc ggggttggcg aagccgccct
     1441 tgtcctggag cgcctgtggc aggtcccagt ggaaaaggtt gagcgtcggc tccaccccgg
     1501 ccgcggcgag ctcgtcgacg aggcgccggt agaagtcgag tccctgctgg ttgaccgtgc
     1561 cgcgaccttc gggcatgacg cgcggccagg acacgctgaa gcggtacgcg tgggcgccca
     1621 gctccgccat caaccgcacg tcgtcgcggt agcggtggta gtggtcgcac gcgacgtcgc
     1681 cggtctcgcc attggctgtc ttgcccggcg tgtgcgagaa cgtgtcccag atggatggcc
     1741 cgcggccgtc ttcgtttgcc gcgccctcta tctggtacgc cgacgccgcg aagccccatg
     1801 tgaagtcggc cgggaaacgc tgcgcgagtc tttcgggatt ggtctcgaag tcggtgtcag
     1861 tcatcgagtc gaaggatacg ttcaccggtt gacgcggccc ggtgatcggt caggggcgcg
     1921 ggagagtcag cgtgaaggtg gcgccgccat cgggcgtcgg cgaatgttcg agcgtgccgc
     1981 catgcgccgc cgcgatcgac gcggcgatgg cgagtcccag gcctgagccg ccgcggtcgc
     2041 gagtgcggcc cgcgtcggcg cgatagaaac gatcgaagac gtgctcgacg tgcgcgggat
     2101 cgatgcccgg cccgtggtcg gccaccgtca gcaccacgga ctgatcaccg gagctgagcg
     2161 aaacctccag cggcgaatcg ggcggcgtgt gcacgcgcac gttggccagc aggttgccga
     2221 ccagctggcg cagccggtcc tcgtcgccgg tcacggtcac gtccgccgcc aacgacgccg
     2281 tgatcggccg gcccgggtcc accgcacgca gatcacccac cgcgtcggac aaaagggcgc
     2341 tcaggctcac ccggtcctgg cgcgggggcc gaccctggtc gagccgcgcc agcaggagca
     2401 ggtcctcgac gagcacgctc atgcgcctgc tctcgctgcc gatccgatcc atcgcccggt
     2461 ccagctcggc cgcttgctcc agcccgccgg cgcggtggag gtcggcatag ccgcgcacag
     2521 tggtcagcgg cgtgcgcagc tcgtgcgacg catcagccac gaactggcgc agcatctgct
     2581 cgctcgcctc cttggcgcgc agcgcctgct gctggctggc gaaggccgtt tcgatctggt
     2641 cgagcatcgc gtcgaaggcg tggcccagct tgcccacctc cgagccgtcg tcgggcatcc
     2701 cgacgcgctg cgataggtcg cccgccgaga tctgctcggc cgtcgcggtc atgcgctcga
     2761 gcgctcgcag gttgcgccgg atgagcagcc agccgatgac caccagcagg acaagcacga
     2821 ccaggccgac caccaccagg gtgcgcacga gcgacacgat ggtgccctcg agcgcgcgcg
     2881 ttggtgccgc gaagacaacg gtcagcacct gcggcggaat gcccagccgg gcacgaactg
     2941 cgaccacgcg ataagtcaac gacccatccg tggccggccg ctcaacgacg gcgccaatcg
     3001 gcacggcctg ggcgccggta atggtgacgt ccggcagcgg gtcgggcgaa gagctgaagc
     3061 ctgacgggaa ggtctggatc cgctggcctt cgcggtcaaa cagaagcacc gcgaagcggc
     3121 gcccggtggc gtccggctgc tgggggccgc ccggcgccac gtcggccgga ctcaccgtga
     3181 gcaggtcgtg gtcaaggtcg gccagcaggt tggctcgggt caagctgacg accgcgccgc
     3241 cggaaacgat gagcgcgagc gcgagcagcg cgcccatcgc cagtacgagg cgcgcgcgga
     3301 gggtcattcc ggggaggggc tagctggctt cggcgcgcaa cgtgtaaccg aagccgcgca
     3361 cagtgcggat cagcggctcg tggccggtat cgagcttgcg ccgcaggtac gagacgtacg
     3421 tctcgaccac gttgtcgtcg ccgttgaagt cgtactgcca gacgtggtcg aggatctgct
     3481 gcttggacag gacgcgctcg gcgttgagca tcaggtagcg cagaaggcgg aactcggtcg
     3541 gcgagaggcg cacttccgtg ccgccgcgcc acacctggtg gcgatcctcg tccatctcca
     3601 ggtcgcggta cttgagcctg tgctcggccg gcgaatcggc agccgtcttc gttcggcgca
     3661 ggagcgcgcc gatgcgcagc gtcagctctt cgagcgagaa cggcttggtc acatagtcgt
     3721 cgccgccgcg gatgaagccg gtgacgcgat cctcttctgc gtcgcgcgcc gtgaggaaga
     3781 tgaccggcac gaaatcgcca tcggcgcgca gccggcggca cacctcgaac ccgtcgatat
     3841 cgggcaacat cacgtcgagc accaccagat ccggcggcga cttggacgcc gactcgagcg
     3901 cggcgttgcc cgtagccgct gtcgccacct ggtagcccat gtagcgcagc gcagtcgaca
     3961 gcagctccgt gatcgtcgcc tcgtcgtcca cgacgagcac gcggccgcgc cgctcggctc
     4021 ttgcgtcgtt gcggacgggg agggcagacg tgttcacggg tggtatttga tgcgcctcac
     4081 cttagaactt gctgtgaatc atgactcagc ccgctgcaac cggccgattc acagcaaatc
     4141 cccagccttc gctgcgctgg agcacaggcc gccgaccgat cttatggctc gaacgacgcc
     4201 gacagctgcg gccccgcgcc ttctctccct cctcctcagg gcgcgccgcg aagtcggcgt
     4261 cgtgccatgt ccagcgcgcg gcgctcctca cgggcgagac cccggtccgc gtttgtttca
     4321 caggcgcgaa gtaaaagcca acttgagtcc ccaagctgcc cacctgttgc acagcgaggt
     4381 atacccagcc aacctgagcg ctcggcagat ccttgcggaa gccacccttg ccctttgttt
     4441 gcaccgccag aaacaaccgc gatgggcgcc cggccgcgcg gcctagtgga cctggccgtg
     4501 gcccggttcg ttgccctgtt cgcgcaggaa gcgctcgatc tcgctgatca gctcgtcgcc
     4561 cgccggtaat cccgactcgt cgaccatctc ctcgaggcgc gtgacgtatg agcgcgtctt
     4621 ctcgt
//
LOCUS       DATBRX010000090         5019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2374924, whole genome shotgun sequence.
ACCESSION   DATBRX010000090 DATBRX010000000
VERSION     DATBRX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374924"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2682)
                     /locus_tag="VIF08_03310"
     CDS             complement(<1..2682)
                     /locus_tag="VIF08_03310"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8135044.1"
                     /translation="MVAEKALVVDPGKAVVGATAQPPVSLAAVGELLAGLNREQRRAV
                     THRAGPQLVIAGPGTGKTEVITRRIAWLIATRRARPAEILALTFTDAAADEMQARVDM
                     LVPYGHAGAAIHTFHALGDRLVREHAFELGLPGDARLINRAEAIVLLRDNLFALGLDR
                     YRPLGDPTRFLGAMVDIFGRAKEEGVDATRLAQHAAQLAGRARDPAEHDAAAALAEQA
                     SAYAAYQHLLGRHGLIDHGDQLGLAARLLAERPNVRRDVVGRYRFLLVDEFQDMNRVQ
                     LDLLLALTPDGGNVTVVGDLDQAIYAFRGAASDNVRRFSAAHPGLEHVVLRRNYRSRQ
                     PIIDAAARLIGHGPRPHIDGADARQVAARRARNPRPVQLDTFVTSTDEADGVAAAAAR
                     RIASGVAPRDIAVLARSNAEIEPLVRSLNLAGLPVRTRTPADFFAQPEIRPLVAFLRC
                     VADPSSSIELYVLATAWPYGLGGERLTELLADSRRAHQPLWAALSRLADDRSLPGDEF
                     VTAVRRLVAHLRAAIDASHQRTAGEVLFDHLRRTSALARLAASDDAAAARSVARFFDL
                     VRSRSALLADARVAALVPHLNELIDVADPVADDGPLDLNAVSVLTVHRAKGLQFRFVY
                     LTGMVDGRFPVHSRPPALGVPWHELHADAASPAEDRLAEERRLCYVAMTRAMDELVLS
                     THLASPAGRGRRRPSPFIAEALDLPVNKAVVQQDQVGPLIGTPPTIPAMPAKASVADL
                     PASSFSFSMLEEYLDCPERYRLRHVIGLPTPPHHSLTYGRAVHAAVAWFHLRVAAGDT
                     PSDADLVAEFRRSWLSEGFLSREHEEARFAAGVRALTAFRAREVATPAPVVAVERPFE
                     FALDGMRIRGRVDRLDEDEGGTVIVDYKTSDVRDQRKA"
     gene            complement(2732..3157)
                     /locus_tag="VIF08_03315"
     CDS             complement(2732..3157)
                     /locus_tag="VIF08_03315"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEY8135045.1"
                     /translation="MIPESMVSSVAVAGIVVGLVGLFVAMWAVLRLRRVSRRLAALEG
                     VSPMAVPVDVDQVNKLGDQLQGAIQRVGVVRYNPFEDTGSNQSFVLAMLDARGDGFVL
                     SSLHSRQATRMFLKPVSRGKADAAVSDEEAEAIRIAAAR"
     gene            3195..3578
                     /locus_tag="VIF08_03320"
     CDS             3195..3578
                     /locus_tag="VIF08_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135046.1"
                     /translation="MTKKVRGAARAHHRPGTRPATDRSNTPRRRQTVVDQPSQLEAAE
                     VAAEDVMEDRPAAAAAVLEANARSHPRHRVKAGSLLATRAATEYVYVAKDMRRILIVT
                     ALLLGIMLVLWLLLVVMRVVPLPFY"
     gene            complement(3601..>5019)
                     /locus_tag="VIF08_03325"
     CDS             complement(3601..>5019)
                     /locus_tag="VIF08_03325"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY8135047.1"
                     /translation="DLSGTAAHAPLAARMRPQALEEFVGQEHLIGEVGALRRMIERGH
                     LASMILWGPPGSGKTTLARLLAESIDTPFATLSAVMSGVADVRAAIAAAREWIAGGGR
                     SSVLFIDEIHRFNKSQQDALLPQVEDGTITLIGATTENPYFEVNSALLSRMRVFRLEP
                     LSNEQLRVVVERALAHPRGLAGRVTLAEDALEHLVDISGGDARSALNVLEAAAVMSDT
                     PTLADIEEAAQQRILSYDRAGDGHYDTVSAFIKSLRGNDVDASLYWLATMVAAGEDPR
                     FIARRLIISASEDVGNADPHALQLATSAAAALDWVGLPEAQYALAQATAYIAAAPKSN
                     RAGTAYWAAMDDVLRHGSLPVPSHLRSATWRERRDFGHGMGYVYPHDYMGADVEQQYL
                     PDELAERRYYEPSDHGYEKTIGDRMALRQAAREEAAGKPRRDPTARPKSDAMKVAGNV
                     MQARDGKKREIAARQKKDASGT"
BASE COUNT          802 a         1732 c         1749 g          736 t
ORIGIN      
        1 cgccttgcgc tggtcgcgca cgtccgacgt cttgtagtcg acgatgactg ttccgccttc
       61 gtcctcgtcc aggcgatcga cacggccgcg gatgcgcata ccgtcgagcg cgaactcgaa
      121 gggtcgctcc actgcgacca cgggcgcggg cgttgccacc tcgcgcgccc tgaacgcggt
      181 caaggcgcgc accccggccg caaacctggc ctcctcgtgt tcacgcgaca agaagccctc
      241 ggacagccag ctgcggcgga actcggcgac gaggtcggca tcggacggtg tgtctccagc
      301 cgcgacgcgc agatggaacc aggcgacggc ggcgtgaacg gcgcggccgt aggtcagcga
      361 gtgatgcggc ggcgtgggca agccgatcac atgccgcaac cggtagcgct ctgggcaatc
      421 gaggtattcc tcgagcatcg agaagctgaa tgacgacgcc ggcaggtcgg cgacgctcgc
      481 ctttgcgggc atggcgggaa tcgtgggcgg cgtccctatt agcggcccga cctgatcttg
      541 ctgcacgacg gccttgttga cgggcaggtc gagcgcctca gcgatgaagg gcgatggccg
      601 ccgccggccg cgaccagccg gactggcgag gtgagtgctc aggacgagct catccatggc
      661 gcgcgtcatg gcgacgtagc acaggcgccg ctcctccgcc agacgatcct cggcgggcga
      721 cgcagcatcc gcgtgcagct cgtgccacgg cacgccaagt gccggcggcc gcgagtggac
      781 cgggaagcga ccgtcgacca tgcccgtcag atagacaaag cggaactgca ggcccttggc
      841 gcgatggacg gtcaacaccg acacggcatt cagatcgagc ggaccgtcgt cggccacggg
      901 atcagcgacg tcgatcagct cgttgaggtg gggcaccagc gcggcgacgc gagcgtccgc
      961 gaggagcgcc gagcgtgagc gcacgaggtc gaagaagcgc gcgacggacc tggcggccgc
     1021 cgcgtcatcg ctggcggcga ggcgcgccag cgcggaggtg cgacgcaggt ggtcgaacaa
     1081 cacctcgccc gccgtccgct gatgcgatgc gtcgatggca gcgcgcaggt gcgccaccag
     1141 gcggcgtacc gcggtcacga actcatcacc ggggagcgat cgatcgtccg ccaggcggct
     1201 gagcgccgcc catagcggct ggtgcgcgcg ccgtgaatcg gcaagcagct cggttaggcg
     1261 ctcgccgccg agcccatagg gccatgccgt cgcgaggacg tatagctcga tcgagctcga
     1321 ggggtcggcg acgcagcgca ggaaggcgac cagcggccgg atctcaggtt gggcgaagaa
     1381 atcggccggt gtgcgagtcc gcacgggcag cccggccagg ttgagcgagc gcacgagcgg
     1441 ctcgatctca gcattgctgc gcgccaggac cgcgatgtcg cgtggcgcga cgcccgaggc
     1501 gatccgccgg gctgcggcag ctgccacgcc gtctgcctcg tccgtcgacg tgacgaaggt
     1561 atcgagctgc actggtcgcg ggttgcgcgc acgccgcgcc gccacctgac gggcgtccgc
     1621 cccgtcgatg tgcggccgag gaccgtggcc gatcagacgc gccgccgcgt cgatgatcgg
     1681 ttggcgcgac cggtaattgc gtcgcaggac gacgtgctcg aggcctgggt gggcagcgct
     1741 gaagcgacgc acgttgtcgc tcgctgcgcc gcggaaggca tagatagcct ggtccaggtc
     1801 accgaccacg gtcacgttgc cgccatccgg cgtgagcgcc agcagcaggt caagctgcac
     1861 acggttcatg tcctggaatt cgtcgacgag caggaagcgg tagcgaccga cgacgtcccg
     1921 gcgcacgttc gggcgttcgg ccagcaggcg cgcggcgaga ccgagctgat caccgtggtc
     1981 gatcagcccg tgccgtccga gcagatgctg gtaagcggcg tatgccgacg cttgttcggc
     2041 gagcgccgcg gccgcgtcat gctcggcagg atcccgcgcc cggcctgcca gctgggcggc
     2101 gtgctgggcc aggcgggtgg cgtcaactcc ttcctccttg gctcggccga agatatcgac
     2161 catggcaccc aggaaacgcg tgggatcgcc caacggccga tagcgatcga gcccgagcgc
     2221 aaacaggttg tcgcgcaaca gaacgatcgc ttcggcgcga ttgatgagac gcgcatcgcc
     2281 gggcaggccg agctcgaacg cgtgctcgcg cacgagccgg tcgcccaatg catgaaaggt
     2341 gtggatcgcg gcaccggcat ggccgtaggg cacgagcatg tcgacccggg cctgcatctc
     2401 atctgccgca gcatccgtga aagtcagagc caggatctcc gcgggacgcg cccgccgcgt
     2461 ggcgatcaac caggcgatgc gcctggtgat gacctccgtc ttgcccgtgc cgggaccggc
     2521 aatgacgagc tgcggcccgg cgcggtgggt gacggcacgc cgctgctcgc gattgagccc
     2581 cgccaacagc tcgcccaccg ctgccaggct gaccggcggc tgggcagtgg caccaacaac
     2641 ggccttcccg gggtcaacaa caagggcctt ctctgcaacc acgcgcccac tgtcgcgcgg
     2701 ccacgtgcca ccttgcgtgg cacaccccta atcagcgcgc ggcggcgatc ctaatggcct
     2761 ccgcctcttc gtccgaaaca gccgcgtcgg ccttgccgcg gctgaccggc ttgaggaaca
     2821 tccgggtcgc ctggcgagag tgcaggctcg atagcacgaa cccatcccca cgggcatcga
     2881 gcatcgccaa cacgaatgac tggtttgagc cggtgtcctc gaacgggttg tagcggacca
     2941 cgccgacgcg ctggattgca ccctggagct gatcgcccag tttgttgact tgatcgacgt
     3001 caaccggcac cgccatgggc gagacgccct ccagcgcggc caaacgacgg ctgactcgac
     3061 gcagtcgcag caccgcccac atggcgacga acagaccgac caggccgacg acgatgccgg
     3121 cgaccgccac gctcgaaacc atgctctcgg ggatcacgga cgacattcta ggcggggcgc
     3181 tagactcgcc cgcgatgacc aagaaagtcc gcggggcagc gcgcgcgcac caccgaccgg
     3241 gcacccgacc ggcgaccgat cgctccaaca ctccgcgtcg gcgtcagacc gtggtcgatc
     3301 agcccagcca gctcgaggcg gcagaggtag cggccgagga cgtgatggag gatcgcccag
     3361 cggccgctgc cgcggtactc gaagcaaatg cgcgcagcca cccgcgccat cgcgtcaagg
     3421 ccggcagcct cctcgccacg cgcgccgcga ccgagtatgt ctacgtcgcc aaggacatgc
     3481 ggcgcatcct gatcgtcacg gcgctgctgc tcgggatcat gcttgtcctg tggctgttac
     3541 tggtggtcat gcgcgtggtg ccgctgccct tctactagag ggcaggcaag gctttctcgc
     3601 ctaggttccg gacgcgtcct tcttctggcg cgcggcgatc tcgcgcttct tcccgtcgcg
     3661 cgcctgcatg acgttgcccg cgaccttcat cgcgtccgac ttgggccgcg cggttgggtc
     3721 gcgccgcggc ttgccggcag cctcctcgcg cgcggcctgg cgcagggcca tccgatcgcc
     3781 gatcgtcttc tcgtagccgt ggtctgaggg ctcgtagtag cgccgctcgg cgagctcgtc
     3841 gggcaggtac tgctgctcga cgtcggcccc catgtagtcg tgcgggtaca cgtagcccat
     3901 gccgtggccg aagtcgcgcc gctcgcgcca cgtcgcacta cggaggtgtg acggcaccgg
     3961 cagcgagccg tggcgcagga catcgtccat cgccgcccag taggcggtgc cggcgcggtt
     4021 cgacttgggc gccgcggcga tgtaggccgt tgcctgggcg agggcgtact gtgcctccgg
     4081 cagccccacc cagtcgagcg ccgcggcagc cgacgtggcc agctgcagcg cgtggggatc
     4141 ggcattgccc acgtcctcgg atgcgctgat gatcaggcgg cgcgcgatga atcgggggtc
     4201 ctcaccggcc gcgaccatcg tcgccagcca atacagcgag gcgtcgacat cgttgccgcg
     4261 caggctcttg atgaacgcgc tcaccgtgtc gtagtggccg tcgccggcgc ggtcgtagga
     4321 caggatgcgc tgctgggcgg cctcctcgat atcggccagg gttggcgtgt cgctcatcac
     4381 ggcggctgcc tcgagcacgt tcagagcgga gcgcgcatca ccaccactga tgtcgaccag
     4441 gtgctcgagc gcatcctcgg ccagggtgac gcgccccgcc agcccgcgcg gatgggccag
     4501 tgcgcgttcc acgacgacgc gcagctgttc gttgctgagc ggctcaaggc gaaagacgcg
     4561 catgcgcgac agcagcgcgg agttgacctc gaagtacggg ttctccgtcg tcgcgccgat
     4621 cagcgtgatc gtgccgtcct cgacctgcgg caatagcgcg tcctgctgcg acttgttgaa
     4681 gcgatggatc tcgtcgatga acagcacgct cgagcggccg ccacccgcta tccactcgcg
     4741 cgcggcagcg atcgcggcgc gcacgtcggc gactccgctc atcactgccg aaagcgtggc
     4801 gaagggtgtg tcgatcgact ccgccagaag ccgtgcgagc gtcgtcttgc cgctgcccgg
     4861 cgggccccac aggatcatcg acgcgaggtg gccgcgctcg atcatccgcc gcaatgcccc
     4921 gacctcgccg atcaggtgct cctggccgac gaactcctcg agcgcctgcg gccgcatgcg
     4981 cgcggcgagc ggcgcgtgcg cggccgtgcc cgacaggtc
//
LOCUS       DATBRX010000091         2783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2151712, whole genome shotgun sequence.
ACCESSION   DATBRX010000091 DATBRX010000000
VERSION     DATBRX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151712"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VIF08_03330"
     CDS             <1..480
                     /locus_tag="VIF08_03330"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEY8135048.1"
                     /translation="SPADAQRVLGTLGPEPLGEELTLAEFRRMLRGRRGRLKTLLMNQ
                     AFLAGVGNIYADEALWRARLHPLRSAANLRADQARHLYVALRDVLNEAIARRGSSVDS
                     YRAPEGSGDMQHYLNVYGRTGKPCPRCGRPTRRMVIGARSTHFCSWCQRLPAAERPA"
     gene            480..2456
                     /locus_tag="VIF08_03335"
     CDS             480..2456
                     /locus_tag="VIF08_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY8135049.1"
                     /translation="MAILRLSNVRREIGDFVILNSINVAVAHGDKIGLVGANGAGKTT
                     LLRIAAGRDQPDSGEVHAKRGLKVGLLTQEANMDAAFIGASSLRAAVRSGAGELEQME
                     ARLRELESAGPPGVESAEYAHLRESFEARDGYTLDVRVDEALSGLGFARADWPRPPTE
                     LSGGQQTRAALARMLVAELDLLLLDEPTNHLDVDAIEWLEKTLAARRDAVVVASHDRA
                     FLDGVVARIWELRDRRIEAFRGNYAAYLAQREERDARSRLEGDVTQQTIKRERELVQT
                     YRSHRKFAKMHEHERRLEAALEEAAAAPKSMRRPALTLDGLARQGAARSGDVALDLEN
                     VVAGYAGRPIVKVPRLEARRGARIGLVGANGSGKTTLLRTIAGELAPLEGYIRFGHKV
                     QTGYLAQIRAEALGGATVLETMMTRGGLDEGPARSYVARFLFRGDDVFKTVDVLSGGE
                     RSRLELALVGLLAANLLLLDEPTNHLDIPAREALESFLRDAPGTVIVVSHDRRLLEAV
                     CTELWIVADGAGETASVVRFVGGFAEWRAAVAAGWTPASSAAAATPPLRASKPLKPEI
                     SKAPTNGKTRPAARATLPRLSKDAYRRRRQVVEDDLTRLGLRKSQLELALSDPRVQAN
                     FVELRRVSSELADVDAALAQAEDAWLMLADQAPR"
     gene            2456..>2783
                     /gene="coaE"
                     /locus_tag="VIF08_03340"
     CDS             2456..>2783
                     /gene="coaE"
                     /locus_tag="VIF08_03340"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY8135050.1"
                     /translation="MAKSNGDSHLHNQGEDRGGNHGDNRPLLIGITGPIGCGKSNVGR
                     VLGELGGTVIDADALARAATAPGGPALAEIRERFGGALFDARGELDRAGLAAIVFEDA
                     AALTDLE"
BASE COUNT          416 a          975 c          987 g          405 t
ORIGIN      
        1 agcccggccg acgcccagcg ggtgctgggc acgctgggtc ctgagccgct gggcgaggag
       61 ctgacgctcg cggagttccg gcgcatgctg cgcggccgcc gcggtcggct caagacgctg
      121 ctcatgaacc aggcattcct cgccggggtg ggcaacatct acgccgacga ggccctgtgg
      181 cgcgcgcggc tccatccact gcgctccgcc gccaacctgc gcgccgacca ggcgaggcac
      241 ctgtacgttg cactgcgtga cgtgctcaac gaggcgatcg cccgccgcgg gtcaagcgtc
      301 gactcgtatc gagcgccgga gggcagcggc gacatgcagc actacctgaa cgtctacggc
      361 cggaccggca agccatgtcc gcgctgcggc cggccgacgc gacgcatggt gatcggcgcg
      421 cgctccacgc atttttgttc ttggtgccag cgcctgcccg ccgctgagcg gcccgcctga
      481 tggccatcct gcggctgtcc aacgtgcggc gcgagatcgg tgatttcgtg atcctgaact
      541 cgatcaacgt cgccgtcgcg cacggcgaca agatcggcct cgtcggcgct aacggtgccg
      601 gcaagacgac gctgttgcgc atcgcggctg gccgcgatca gcccgatagc ggcgaggttc
      661 atgccaaacg cggcctcaag gtgggcctgc tgacccaaga ggccaacatg gatgccgcct
      721 tcatcggcgc atcgagcctg cgcgcggccg tgcgctcagg cgctggggaa ttggagcaga
      781 tggaagctcg cctgcgtgag ctcgaatcgg ccggtccgcc aggcgtggag tcggccgagt
      841 atgcccatct gcgcgagagc ttcgaggcgc gcgacggcta cacgctcgat gtgcgcgtgg
      901 atgaggcgct gtccggcctg ggctttgccc gcgcggactg gcccaggcca ccgaccgaac
      961 tgtccggtgg acagcagacg cgcgccgccc tggcacgcat gctcgtcgcc gagctcgatc
     1021 tgctgctgct cgacgagccc acgaaccacc tcgacgtcga cgcgatcgaa tggctcgaga
     1081 agaccctggc cgctcggcgt gacgccgtcg tcgtggcgtc acacgaccgt gcgttcctcg
     1141 acggtgtcgt agcgcgcatc tgggagctgc gcgaccgccg gatagaggca ttccgcggca
     1201 actatgccgc gtacctcgcg cagcgcgagg agcgcgatgc gcggtcacgg ctcgaagggg
     1261 atgtcacgca acagacgatc aagcgcgaac gcgagctggt gcagacgtac cgcagccacc
     1321 gcaagttcgc caagatgcac gagcacgagc gtcgcctgga ggccgcgctc gaggaggccg
     1381 cggccgcgcc caagtccatg cgccggcccg cactaacgct ggacgggctc gcgcgccagg
     1441 gagctgcgcg ctccggcgac gtggcgctcg acctggagaa cgtcgtcgct ggctacgccg
     1501 ggcggcccat cgtcaaggtt ccgcggctgg aggcgcgacg cggggcgcgc atcgggctgg
     1561 tgggcgccaa cgggtcgggc aagaccacgc tgctgcgcac catcgccggc gagttagcgc
     1621 cgctcgaggg ctatatccgc tttggccaca aggtgcaaac cggctacctc gcccagatcc
     1681 gcgccgaggc gctcggcggc gcgaccgtgc tcgagacgat gatgacgcgc ggcggcctgg
     1741 acgaaggccc cgcccgctcc tatgtggcgc gtttcctgtt ccgcggcgat gacgtcttca
     1801 agacggttga cgtcctgtcc ggcggcgagc gttcgcgcct cgagctggcg ctggtcggac
     1861 tgctcgcggc gaacctgctg ctgctcgacg agcccacgaa ccacctcgac atcccggcgc
     1921 gcgaggcgct cgagtcgttc ctgcgtgacg cacccggcac ggtcatcgtc gtgtcgcacg
     1981 accggcgcct gctggaggcc gtctgcaccg agctgtggat cgttgccgac ggcgcgggcg
     2041 agacggcgag cgtggtgcgc ttcgtgggtg gcttcgccga gtggcgcgct gcggtcgccg
     2101 cgggatggac tccggcttcg tccgcagcgg cggccacgcc cccgctacgt gcctccaagc
     2161 cgctcaagcc ggagatctcc aaggcaccga ccaacggcaa gacccgcccc gccgccaggg
     2221 cgaccctgcc gcggctgtcg aaggacgcct accgccggcg gcgccaggtg gtggaggatg
     2281 acctgacgcg gctggggttg cgcaagagcc agctcgagct cgccctgtcc gacccgcgcg
     2341 tgcaggccaa tttcgtcgag ctgcgccgcg tcagcagcga gctggccgac gttgatgccg
     2401 cactcgccca ggcagaggat gcctggctga tgctggccga ccaggcgccg cgctgatggc
     2461 caagagcaat ggcgacagcc acctccacaa ccaaggtgaa gaccggggtg gcaaccatgg
     2521 cgataaccgg ccgctgttga taggtatcac cggtccgatc ggctgcggca agtccaacgt
     2581 cggccgcgtt ctcggtgagc tgggcgggac cgtgattgat gccgacgccc tcgcgcgcgc
     2641 cgccaccgca ccaggcgggc ccgcgctggc cgagatccgg gagcgcttcg gtggcgccct
     2701 ttttgatgct cgcggcgagc tcgaccgcgc gggcttggcg gcgatcgtct tcgaggacgc
     2761 ggccgcgctc accgacctcg agc
//
LOCUS       DATBRX010000092         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3045700, whole genome shotgun sequence.
ACCESSION   DATBRX010000092 DATBRX010000000
VERSION     DATBRX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045700"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..606
                     /locus_tag="VIF08_03345"
     CDS             121..606
                     /locus_tag="VIF08_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8135051.1"
                     /translation="MKTPDGDLRELLAATWLTHELDAPARNALAAIGRIVSVRAGSVL
                     MREGETNDVMAIVLDGRVSLRMRVPERGQVSIVTIEPGDVVGWSAMVPPYRATSTAVV
                     LTDTELAQFDGPRLRELIATNTELAAQLYPVLLKAVARRLEGTRLQLLDLFSQRSAEP
                     W"
     gene            603..>2329
                     /locus_tag="VIF08_03350"
     CDS             603..>2329
                     /locus_tag="VIF08_03350"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8135052.1"
                     /translation="MSAVEPQTFFLPRQQLGRLMERLQDGGRQVIGPTVRDGAVMLDE
                     IETADQLPIGWGIDVAPGKARLVKRDDERVFDQPPGPTSWKRWTFPPRLTAYAWNDAN
                     DAADTADTSAGATRSPRPTPAQVGPLAFLGARACEISALHVQDKVLLEGPVVDGDYAA
                     RRRDNLIVAVECAVAGGTCFCTSMGTGPEVTDGFDLALSELDDGFVVRVGTDAGLAAL
                     DGLALAPASGDQTTAAATQVAAVRENLGAPIPMEGLPARLVAAADSPRWAQIAERCLA
                     CTNCTLVCPTCFCTSISQRSDLDGAGASAERRWDSCFTLDFARVAGGNFRTRVADRYR
                     QWLTHKFGTWWSQFGSSGCVGCGRCIAWCPVGIDVREELLAVAPAPQTPVIEALIESP
                     RIEPLPAVPVLPAMPVPPAVPQPARPMPWRMVEVTDRRRETHDVVTLTLGTDDPALLA
                     GRPGQFVMVGLPAVAVPPISVSRFHPDGLELTIRAAGPATAALVNLERGDKVALRGPM
                     GRGWPVELAEGRDVMVITGGIGLAPLRPLLDHMLARRDRIARIHLAYGARTPGDRLYT
                     DELDRLAASG"
BASE COUNT          331 a          850 c          803 g          345 t
ORIGIN      
        1 gcccgtctgg cggcccagtt cgtccgtttg gcggggcctt cgtgccaaac ggcacgcgtc
       61 cggctcgcgg ccgcccaccg aggattgggc agaagggagt tccggttgca gaaccagccc
      121 gtgaagacac ctgacggcga cctgcgcgag ctgctcgcgg ctacctggct cacccacgaa
      181 cttgatgccc cggcgcgcaa cgccctggcg gcgatcggcc gcatcgtgtc agttcgggcc
      241 ggcagcgtcc tgatgcgcga gggcgagacg aacgacgtca tggcaatcgt gctcgatgga
      301 cgcgtgtcgc tgcggatgcg cgtgcccgag cgcggccagg tatcgatcgt cacgatcgag
      361 ccgggcgacg tcgtgggctg gtcggcgatg gtgccgccgt accgcgcgac atcgaccgca
      421 gtggttctga cagacacgga gctcgcccag ttcgacggac cgcgactgcg cgagcttatc
      481 gccaccaaca ccgagctggc tgcccagctc tacccggtgc tgctcaaggc ggtcgcgcgc
      541 cgcttggaag ggacgcgcct gcagctgctt gacctgttca gccagcgctc ggccgaacca
      601 tggtgagcgc cgtagagcca caaacgttct tcctgccgcg gcagcagctg ggcaggctca
      661 tggagcggct ccaggacggc ggacggcagg tcatcgggcc gacggttcgc gacggcgccg
      721 tgatgctcga cgagatagag accgccgatc agctgcctat cggctggggc atcgatgtcg
      781 cgccaggcaa ggcgcgcctc gtcaagcgcg acgacgagcg cgtctttgac cagccgcccg
      841 gcccgacatc gtggaagcgc tggaccttcc cgccgcggct gaccgcctac gcctggaacg
      901 acgcaaacga cgcggccgac acggccgaca ccagcgcagg cgcaacgcgc tcacccaggc
      961 ccaccccggc acaggtcggg ccgctcgcgt ttcttggtgc tcgcgcctgt gagatcagtg
     1021 cgctccacgt ccaggacaag gtgctgctcg agggaccggt ggtcgacggc gactacgccg
     1081 cgcgacggcg cgacaacctc atcgtcgcgg tggagtgcgc ggtcgccggt ggaacctgct
     1141 tctgcacgtc gatgggcacc gggccggagg tgaccgacgg gttcgacttg gcgctctcgg
     1201 aactcgacga cggtttcgtc gtgcgcgttg gcacggacgc cggactcgcc gcactcgatg
     1261 gcctggcact cgcgccggcg tcgggcgacc agacgaccgc cgcggccacc caggtcgctg
     1321 ccgtccgcga gaacctcggc gcgccaatcc ccatggaagg tctgcccgcc cgattagtgg
     1381 ctgctgccga cagtccgcgc tgggcccaga tcgccgagcg ctgcctggcc tgcaccaact
     1441 gtaccctcgt ctgcccgacc tgcttctgca ccagcatcag ccagcgttcc gacctcgatg
     1501 gcgccggcgc atccgccgag cgtcgctggg actcgtgttt cacgctcgac ttcgcgcgcg
     1561 tcgccggtgg caacttccgg acgcgtgtcg ccgaccggta ccgccaatgg ctgacccaca
     1621 agttcggcac ctggtggtcg cagttcggca gcagcggctg tgtcggttgc gggcgctgca
     1681 tcgcgtggtg cccggtgggc atcgacgtgc gcgaggagct gctcgccgtg gcgccggcgc
     1741 cgcaaacgcc agtgatcgaa gcgctgatcg aatcgccacg gatcgaaccg ctgccggccg
     1801 tgcccgtgct gccggccatg cccgtgccgc ccgcagtgcc ccagccagcc cggcccatgc
     1861 cctggcgcat ggtcgaggtg accgaccgcc ggcgcgagac gcacgacgtc gtgacgctaa
     1921 cccttggcac cgacgatccg gcgctgctcg ccggccggcc cggccagttc gtcatggtcg
     1981 gactgcctgc ggttgccgtg ccgcccatct ccgtatcgcg cttccaccct gacgggctcg
     2041 agctgaccat ccgcgccgcc gggccggcga ccgcggcgct ggtcaacctc gagcgcggcg
     2101 acaaggtcgc tttgcgcggc cccatgggcc gcggctggcc cgtcgagctc gccgagggcc
     2161 gcgacgtgat ggtcatcacc ggcgggatcg gcctcgcgcc gctacggccg ctgctcgacc
     2221 acatgctggc gcggcgcgac cggatcgcgc gcatccacct cgcctacggc gcgcgcacgc
     2281 cgggcgatcg cctctacacc gacgagcttg atcggctggc cgcgagcgg
//
LOCUS       DATBRX010000093        14702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2262790, whole genome shotgun sequence.
ACCESSION   DATBRX010000093 DATBRX010000000
VERSION     DATBRX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14702
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262790"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VIF08_03355"
     CDS             <1..501
                     /locus_tag="VIF08_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135053.1"
                     /translation="RSADWAAIEFADIGELTDVIRIGGLANQKAPRIQAALRQIREQR
                     GDYSLEFLGDMTPPEALAWLTQVNGIGRKTASVVLLFSFGMPLMPVDRHIERVAMRIG
                     LIPRKIANAEQMHDMLQNQLTADTVHEAHVNLITHGRQTCHAQRPEHERCAIADRCRY
                     FNPKAP"
     gene            complement(513..2741)
                     /locus_tag="VIF08_03360"
     CDS             complement(513..2741)
                     /locus_tag="VIF08_03360"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY8135054.1"
                     /translation="MTASTTSLGRTAVFALVGARASRWVFVAALALSSFLMFTLELLA
                     GRVVLPVFGGAPGVWATALCFFTALLFIGYSYAHVLVTRLDSRRAATLHLVVAIVLVG
                     ATFLGTTGAADLRDANLPEAVNVLLALLVIAGPPAFLLASTTPLLSAWFAGGARDAWW
                     LYAASNAASLLALITYPFVVEPFMPLSAQWVVLRLGLLAFVAVLAAIALSRRADAGTN
                     IAAQSRALAAPTALGWRRVAHWLFLAFVPAGLLTATTSFLTTDLVSAPLIWIGPLAIY
                     LASFVVAFSERGRRVLPAVERLVPAAATFLWLPYVAPVGWPAVPLVVIVLGSFAVLAT
                     AVHGRLAQSRPGSSQLTHFYLLLSLAGVLATATVGVAAPMLLPDIYEFPALLVLALVA
                     LALAGRQTMGEVLRQPLGAARDLAARLIPYVAVGAALILFTGTADAGVVALLTLGGMV
                     VAFALTPRILLPTTGAAMVAALMVLTATPGATELFVGRSFFGVSKVVTVDGQQRLYSG
                     TTLHGVQFTDARSSDPTSYYVLGSPLSDVFADLRSRTTGADIGVVGLGAGVVAAYTET
                     ADRLTYFEIDPLVVKLASDPHYFSYLADAPAQVDIVLGDGRLTLADQPGGAFDVLVLD
                     AFSSDSVPPHLLTAEAIASYMRVLRPGGVLAFNLSNRYYDLAPAVAATARSLGLDAQT
                     RAFTPDAEQVTSHAATASLWLIVGQSGDIAAFAERGWSQVTRPGPVLSDDYPDITRVL
                     RL"
     gene            2852..4504
                     /locus_tag="VIF08_03365"
     CDS             2852..4504
                     /locus_tag="VIF08_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8135055.1"
                     /translation="MAEFTQLVEDFLKNEYETSPVAATALGLTDYDERLDDMSEGAYR
                     KRDADAIEWIARFQAVDPSTLDFDNQIDRDLAIATLRGRTIAADWQVWKRDPTIYSGP
                     ILNGLFYLFLNRLRPTADLVDAAVARLEQVPRALEQAAVNLDPALAAQLILERGLGSA
                     KAGAGYVRSMLVEEADTDEQRDRLRVAGAVAGDAFDAFAAHLEKLSGQASGTWVYGEE
                     RYSRQLREREVLDFDARSLRDMGQVEYDRLDAEMRALAREAKGTDDWHAVLSEANEDH
                     PPTEEAMRRSYAEWTQKSRDFLAATGLVTLPAGERCEVEPSPVFTRPLLAVASYSGPP
                     AFSNSLTGHFFVPIAPDGTSAEDIQKRLASNSYSGIPTTAVHEAYPGHHWHISWSKVH
                     APRLRRVLQTPYFSEGWALYAERVMRERGFFEDPLHVLNHLEATIFRAARIVVDTSLH
                     MGEMTYEEAVTYMTQKTAITEPTARAEVGRYCSWPTQASSYLTGCLEILRIRRRYLDA
                     RGLSGVPPADAPMDVIRDFHDKICGSGRLPIGLAERAVMTGL"
     gene            complement(4701..4994)
                     /locus_tag="VIF08_03370"
     CDS             complement(4701..4994)
                     /locus_tag="VIF08_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135056.1"
                     /translation="MTDPRPRVHLTARALTAGSLSAAGLLGLAFLLGLAGAVDLGRLV
                     GNIGVMVLLATPVAGLLTTWWELRVLRPTHAWLATAVLLVLGLATIVALAARA"
     gene            complement(4991..5821)
                     /locus_tag="VIF08_03375"
     CDS             complement(4991..5821)
                     /locus_tag="VIF08_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8135057.1"
                     /translation="MIPLELLALLAALGAGVFGAIAGVGGGIIVVPLLSVALGVPLHH
                     AIAVSLLGVIAISATASANYLEAGFVSRRIGLTLLVATTLGGIVGGFIAGLVDARLLA
                     ALFGIVLIAVALRMLFGREPVEPTTVEDLGPLEFDATYVEPRTGEEVAYRVRNFGIGS
                     AVSVFAGMLSGLLGVGGGIVNVPTMNSLMGVPIRVATATSTYMLGATAAASAVLYFSR
                     GEVEPALAGAVVVGSVVGARIGARFHHVLPQRALTLIFVGVAAFFAVQMLLRALGGGA
                     "
     gene            5855..7081
                     /locus_tag="VIF08_03380"
     CDS             5855..7081
                     /locus_tag="VIF08_03380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY8135058.1"
                     /translation="MPQTAAVGRGGPLVGLRVLELSSVLAGPYCAMVLADLGADVIKV
                     EPLDGDATRGYGPPWINPDAPASERSAVYFQSVNRNKRSLRLDLASEGGREVVRRLIR
                     KSDVLVENFRPGGMDRIGLGDAALNELNPKLVRLSITGYGPEGPDSQKPGYDFVAQAV
                     GGLMSVTGFPDAQGGQPTKLGVAITDIVTGLLGAVGVLAALRSGVGQRIDVSLLESTL
                     ALLINQAHNAFATGEQPPRLGNAHPNIVPYETFATADGEIVVAVGSERQWPKLCGELD
                     LPELATDERFADNGARVRNRDVLRPILAARLAEADSTHWLHRLDEAGIPCGPVNSVLA
                     AFEQPQAVAREMRVEVEHPKLGRVPQVGLPYKLSATPASIRTAPPLLGEHSTEVLVEI
                     GYSTAEIAALRSGGVI"
     gene            complement(7078..7884)
                     /locus_tag="VIF08_03385"
     CDS             complement(7078..7884)
                     /locus_tag="VIF08_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135059.1"
                     /translation="MTSAAGGNDRLYRGLLRLYPADYRAQFSDQMVQLFTDHKREVGA
                     TRAWLRAPIDVITTAVGEHLRRNRTVAHSMTVAPTPVTRLLGLVGLLGGASLLAAFVV
                     DIPNDANYIRLFVFGIGGLTVVTAIHLRQRQRSPGLALVGAIPALVFGTWMFVMELLV
                     IGREPPVFGGTFGWVFFWASIASWLGYAWFGVVTFLLHQVTRIGAAALVVGSLLGILG
                     IDRLGLASSTEPTIWNTVALTGVFLHGLAWVILGLDVAFRRRPAPPTASA"
     gene            complement(7881..8237)
                     /locus_tag="VIF08_03390"
     CDS             complement(7881..8237)
                     /locus_tag="VIF08_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY8135060.1"
                     /translation="MSTAHSAAAPPPQVSRTAILIMLAIGPEERHGYAIMQEVARITD
                     GATRLGPGGVYTTIRRLLEDGLIEESDERPDPEMDDQRRRYYRLTGLGRTVVAAEARR
                     LQSLVAAAKPWALEAR"
     gene            8307..8381
                     /locus_tag="VIF08_03395"
     tRNA            8307..8381
                     /locus_tag="VIF08_03395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8339..8341,aa:Thr,seq:cgt)
     gene            8415..9026
                     /locus_tag="VIF08_03400"
     CDS             8415..9026
                     /locus_tag="VIF08_03400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8135061.1"
                     /translation="MKIRLILAGAMALAIAAPALAKTPYTYRYPVTPGYTSGMANTHG
                     GGYPAADLIIKPRCGTAARSPVDGVVLEADRRNLWAQGFRGGAYRGGIFVSILGEDGV
                     RYYMSHFASIPEAIKRGVTVTAGQTVGIVGRTGRAGSCHIHFGISPPCFRKGDWWIRR
                     GVVWPQPYLRSWKKADIADLSPQARVARWLRANGCPATEADLP"
     gene            9065..9139
                     /locus_tag="VIF08_03405"
     tRNA            9065..9139
                     /locus_tag="VIF08_03405"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9097..9099,aa:Thr,seq:cgt)
     gene            complement(9165..11897)
                     /locus_tag="VIF08_03410"
     CDS             complement(9165..11897)
                     /locus_tag="VIF08_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8135062.1"
                     /translation="MDSALDRRLVAVMFTDMVGYSAQMQSDERVAVLKRDQYIAAVEQ
                     EHAAFGGTIVQRLGDGTMSMFPSALAAVSAAVAAQRRLSAADVSVRIGIHVGEVVVER
                     ERLTGDAVNVAARIESFAVPGGVLLSDAAREQLRNRSDVDLVSLGRFKLKSIGQPVEL
                     YAVAGDGLVLPDAQTLEGKGERFASLPSNLPEPASALVGRSRELQQIVELVRAQRIVT
                     VTGPGGVGKTRLLVELGRTLTPEFLDGVSFVSLADVLDPVGFVPALAAALDVKEAEER
                     SLREGVVALIGDKTALLLLDNMEQIVGAAADVAHIAQSCPNLRIVTTSRTPLRLAAER
                     EYPLPTLDVPDADQKDIAGSLMDYSATALFIERARAAKSSFEVTPENARAIADICRRL
                     DGLPLALELAAARLRILSPQALNERLGDALNVLTSGPRGAPERQQTLRATVEWSHSLL
                     TQPDQKLFRRMAVFVGGCAVDDFEAICADAGESALDGIESLVDKALVQVDGHTGRLRM
                     LQTIGDYAREQLRASAEESATAMRHAQRYAHVALAIRDGIESTDQVGALARGVADEGN
                     LMSALDTRLAAAGTGDTGAVQAGLQMCGDLYFYWHLRGKNVTAKDYTLAFLDADKAGL
                     PTVARAGALISAGLSSWVLGEFKLAADQCNEAYLAAAHLDAEREQAVAMLLEGVALLG
                     LDLQAGLRLMGECVTLSRRAGFTWCLGMALAFDGILRAVSGDSESAQVSLAEALQIQQ
                     TLGDIEGIGLTLGARASIAAAQGDLRLALELYTQARDAFESIGDRAEEARILSEMAWT
                     YLRHGDAATARGYFFDSVNAYDAIGSVRGVGLSMVGLASTLAAEDATRAVQIAAAAEV
                     YAAQEGIVNVYSDETPGREFVDTARASLAHDEAALTTESGRRLSMKAAIELARA"
     gene            12016..12537
                     /locus_tag="VIF08_03415"
     CDS             12016..12537
                     /locus_tag="VIF08_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135063.1"
                     /translation="MTIDLNDFPEILTPRLRLRAWTDADADQLADIYADPESVRYLRY
                     YDRAGTRAQVQRFRDHWDEHGFGLWAVEHRETGRFIGRIGLMHHDDWSASPHDAEVGW
                     VIAREMWGQGLASEGGAAALDFGRKKGLNHFVSIAHVDNAASQRVMEKLGLTRQGVTV
                     WRDSPVVWFATDR"
     gene            12545..13441
                     /locus_tag="VIF08_03420"
     CDS             12545..13441
                     /locus_tag="VIF08_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135064.1"
                     /translation="MTRIALATCAALPDLDPDEQLLLEPLRALGVDVQPAVWDDEAVD
                     WASFDLVVIRSTWDYTARRDEFLAWAESVPRLVNPARVLRWNTDKHYLQDLASAGVPV
                     VPTDWLESGNAVILPATGRHVLKPSVGAGSVDAALFDLAVAEQAGLARAHAERLVAAG
                     QTVMVQPYLELIERHGETALIFYGGQFSHAAAKGAMLVSQTEMVAGLYKAELIKPRDA
                     TAAEIEIARAALSAAPIAAEELAYARVDFIPAADGSPLVIELELTEPSLFLGQAPGSA
                     ERLARHLLERASEGTGVEAGQT"
     gene            complement(13668..>14702)
                     /locus_tag="VIF08_03425"
     CDS             complement(13668..>14702)
                     /locus_tag="VIF08_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY8135065.1"
                     /translation="ATPTSGPTTPVVPTPTITPIAEDATTWRELTEVASGPSARTDHT
                     WTAGWAVGAEGDRPVAYLFGGRTADGPSNDLWSFDLETYSWSELQPAGPPPAPRFGHT
                     ATMVPGVGLVVWSGQGDGFFDDIWAYDPGVDAWQQLPSLGAVPDARYGSCASLGPDGR
                     LWVSHGFTQDDGRFDDTRAYDFATGEWTDETPAGDRPVKRCLHDCYWSTQDTLVLYGG
                     QTTGVPALGDIWSYDASAQTWTEGPDSGAPPRQLYALASSSRGTSPFDAIAFGGGSLD
                     GGYLSDLVQISSDSVEAFSSELPNSPSARSGASLIQVGGIASTYLLFGGQAANGLFGD
                     LWQLQGYWLP"
BASE COUNT         2654 a         4983 c         4900 g         2165 t
ORIGIN      
        1 cggagtgcgg attgggccgc gatcgagttc gccgatatcg gcgagctgac cgacgttatc
       61 cgaatcggcg gcctggccaa ccagaaagcg ccgcgcatcc aggccgcgct gcgccagatc
      121 cgcgagcagc gcggtgacta ctcactcgag ttcctgggcg acatgacgcc gcctgaagcg
      181 ctcgcatggc tgacgcaggt caacggcatt ggccgcaaga cagcgtcggt ggtgctgctg
      241 ttctcgttcg gcatgccgct catgccggtt gatcggcaca tcgagcgtgt cgccatgcgc
      301 atcggcctca tccccaggaa gatcgccaat gccgagcaga tgcacgacat gctccagaac
      361 cagttgaccg ccgacaccgt gcacgaggcg cacgtcaacc tcatcaccca tgggcgccag
      421 acgtgccacg cccagcggcc ggagcacgaa cggtgtgcca tcgccgaccg ctgccgctac
      481 ttcaacccca aggcccccta gagccaaacg ccctagagcc gcagcacccg ggtgatgtcg
      541 gggtagtcgt cggaaaggac tgggcccggt cgcgtcactt gagaccagcc gcgttcggcg
      601 aacgctgcga tgtcgccact ctgcccgacg atgagccaca gcgacgcggt cgccgcgtgg
      661 ctcgtcacct gttcggcatc aggcgtgaac gcgcgtgtct gcgcgtcgag gcccaggctg
      721 cgcgccgtgg cggcgacggc cggcgcgagg tcgtagtaac ggttcgagag gttgaacgcg
      781 agcacgccgc cgggccgcag gacgcgcatg tagctcgcga tcgcctcggc cgtgagcagg
      841 tgcggcggca cgctgtcgga cgagaaggcg tccaacacca gcacgtcgaa ggcgccccct
      901 ggctgatcgg ccagggtcag acggccatcg cccagcacga tgtcaacctg cgcaggtgca
      961 tcggccaggt agctgaagta gtgcgggtcg gacgccagct tcaccaccag cggatcgatc
     1021 tcgaagtagg tcagccgatc ggctgtttcc gtgtacgccg caacgactcc cgcacccagc
     1081 cccaccacgc cgatgtcggc accggtcgtg cgtgagcgca ggtcggcgaa gacatcgctg
     1141 agcggactgc cgagcacgta gtagctcgtc gggtcggacg agcgcgcgtc ggtgaactgg
     1201 acgccgtgca gggtcgtgcc cgaatacagc cgctgctggc catcgacggt gacgaccttg
     1261 ctgacgccga agaacgaccg gccgacgaac agctcggttg ccccgggcgt cgcggtcagc
     1321 accatcagcg cagcgaccat cgccgcgccc gtcgtcggca ggaggatgcg cggcgtgagc
     1381 gcgaaggcca ccaccatgcc gcccagcgtg aggagcgcca cgacgcccgc gtccgctgtg
     1441 ccggtgaaga ggatgagcgc cgcgccaacc gccacgtagg ggatgagccg cgcggcaagg
     1501 tcacgtgccg cgccaagcgg ttgacgcagg acctcgccca tcgtctgacg gccggccagc
     1561 gcgagggcaa ccagcgccag gacgagcagc gccgggaact cgtagatgtc tggtagaagc
     1621 atcggcgccg cgacgccaac ggtcgcggtc gcgaggacgc cggcgagcga gagcagcaga
     1681 tagaaatgag tcagctggct cgacccaggc cgcgactgtg cgagccggcc gtggaccgcg
     1741 gtcgcgagga cggcgaatga gccgaggacg atgaccacca gcggcacagc gggccagccg
     1801 acaggcgcga cgtagggcag ccacaggaaa gtggccgcgg ccggcacgag ccgctcgacc
     1861 gccggcagca cacgccggcc gcgctccgag aacgccacca cgaagctcgc caggtagatc
     1921 gccagcgggc cgatccagat caacggcgcg gacacgagat cggtcgtcag gaagctcgtc
     1981 gttgccgtca acaggccggc gggcacgaag gccaggaaga gccaatgcgc cacgcgccgc
     2041 cagccgagcg cggtcggcgc cgcgagcgcc cggctctgag ctgcaatgtt ggttccggcg
     2101 tcggcgcggc gtgagagcgc gatggccgcc agcaccgcga cgaaagcgag caaacccaga
     2161 cgaaggacga cccattgcgc ggagagcggc atgaaaggtt cgaccacgaa cgggtaagtg
     2221 atcagggcca gtagtgaggc cgcgttcgac gccgcgtaga gccaccacgc gtcgcgcgcg
     2281 ccgccggcaa accacgcgga gagaagcggt gtcgtcgagg caagcagaaa cgcgggcggc
     2341 ccggcgatga cgagcagcgc caacagcacg ttgactgcct caggcaggtt cgcgtcacgc
     2401 aggtccgcgg cgccggtcgt cccaaggaag gtcgcgccta ccaggacgat cgccacgacc
     2461 aggtggagcg tcgccgcgcg gcgcgaatcg agccgcgtca ccaggacatg ggcgtacgag
     2521 tagccaatga acagcagcgc ggtgaagaaa cagagggccg tcgcccagac cccgggcgcc
     2581 ccgccgaaga ccggtaacac cacgcggccc gcgagcagtt cgagcgtgaa catgaggaac
     2641 gacgacagcg ccagcgctgc gacgaagacc caacgcgaag cgcgcgcgcc aacgagcgcg
     2701 aacacggcgg tgcggccgag gctggtggtg ctggctgtca ccggccgatg ctatgtagcg
     2761 tcgaccattc cgccgatagg acgtttgggc cagagggctg cggccctgct ggccacccgc
     2821 gacaatgccg aacgaaccgg aggtgcacca cttggccgaa ttcacgcagc tcgtcgaaga
     2881 cttcctgaag aacgagtacg agacgtcgcc tgtcgccgcg accgcactcg gcttgaccga
     2941 ctacgacgag cggctcgacg acatgtccga gggcgcgtac cgcaagcgcg acgccgacgc
     3001 catcgaatgg atcgcccgct tccaggccgt ggatccgtcg accttggact tcgacaacca
     3061 gatcgaccgc gacctcgcaa tcgccactct gcgcgggcgg accatcgccg ccgactggca
     3121 ggtctggaag cgcgacccca ccatctactc gggccccatc ctgaacggcc tcttctacct
     3181 gttcctcaat cgcttgcgac cgaccgccga cctggtcgac gccgccgtcg cgcggctcga
     3241 gcaggtgccg cgcgcgctcg agcaggccgc ggtcaacctg gatccggcgc tggcggcgca
     3301 gctgatcctc gagcgcggcc tgggctcagc caaggccggc gcggggtacg tccgctcaat
     3361 gctggtcgaa gaagccgaca ccgacgagca acgggaccgc ctgcgcgtcg cgggtgccgt
     3421 cgccggggac gcctttgacg cgttcgccgc tcacctcgag aagctctctg gccaagcctc
     3481 gggaacgtgg gtctacggcg aggagcgcta cagccgtcag ctgcgcgagc gcgaggtcct
     3541 ggatttcgat gcccgctcgc tgcgcgacat gggccaggtc gagtacgacc ggctcgacgc
     3601 ggaaatgcgc gccctggccc gggaggcgaa gggcacggac gactggcacg cggtcctcag
     3661 cgaggccaac gaggaccatc cgcccaccga agaggcgatg cgccgctctt acgctgagtg
     3721 gactcagaag tcgcgcgact tcctggcggc aaccggcctg gtgacgctgc ccgccgggga
     3781 gcggtgcgaa gtcgagccgt cacctgtctt cacccggccg ctcctcgcgg tcgcgtcgta
     3841 cagcggcccg cccgcgttca gcaacagcct caccggccac ttcttcgtcc cgatcgcgcc
     3901 cgacggcacg agcgccgagg acatccagaa gcggctcgcc tcgaactcgt acagcggcat
     3961 ccccacgacc gccgtgcacg aggcatatcc gggccaccac tggcacatca gctggagcaa
     4021 ggtccacgcg ccgcggctac ggcgcgtcct gcagacgccg tacttctcag agggctgggc
     4081 gctgtacgcc gagcgcgtca tgcgcgagcg cggcttcttc gaggatccgc tgcacgtcct
     4141 caaccacctc gaggcgacga tcttccgcgc ggcgcgcatc gtcgtcgaca cgtcgctcca
     4201 catgggtgag atgacgtacg aggaagcggt gacctacatg acccagaaga ccgcaatcac
     4261 cgagccgacg gccagggccg aggtcggccg ctactgctct tggcccaccc aggcgtcgtc
     4321 ttacctcacg ggctgcctcg agatcctgcg catccggcgc cgctacctgg acgcgcgcgg
     4381 gctgtccggc gtgcccccgg cagatgcccc gatggacgtc attcgtgact tccatgacaa
     4441 gatctgcggc agcggtcggc tcccgatcgg cctggctgaa cgtgcggtga tgaccggcct
     4501 gtagtaacga gaccgttacg cgcctgcaac ctcgtgggcg cggccggaat gggccggaaa
     4561 agggcgcgtc cgtagtctga cggcacacca atcgaacatc tactcattgc gaggcgtctg
     4621 gtgacggggc gctgccgcaa gagaaaatgc acggcccggg ccccctcccc gggtcgtgct
     4681 cttttatgta ggtctctggg tcaggcccgg gcagccaggg ccacgatggt tgccagacct
     4741 agcaccaaca gcactgcggt ggccagccag gcgtgggtcg gacgcaacac ccgcagctcc
     4801 caccaggtgg tcagcaggcc cgcgacgggc gttgccaaca ggaccattac cccgatgttg
     4861 ccaacgagcc ttccgaggtc tacggcgccg gcaagcccaa gcaggaacgc cagcccgagc
     4921 aggcctgctg cactcagcga gccagctgtc agggcgcgcg cggtcaggtg aacccgcggg
     4981 cggggatcgg tcatgcgccg ccgcccagcg cacgcagcag catctgtacg gcgaagaacg
     5041 cggcgacgcc gacgaagatc agggtcagcg cacgctgcgg caacacgtga tgaaatcgcg
     5101 caccgatgcg cgcgccgacg acgctgccca ccacgaccgc gccggccaat gcaggctcaa
     5161 cctcgccgcg gctgaagtac aggacggcgc tggcagccgc ggtcgcgccc agcatgtagg
     5221 tgctggtcgc ggtcgcgacg cggatcggca cgcccatcag cgagttcatc gtcggcacgt
     5281 tgacgatccc gccgccaacg ccaagcaggc cgctcagcat gccggcgaac acggacaccg
     5341 cggagccaat cccaaagttg cggacccggt acgcgacttc ttctccggtc cgcggctcga
     5401 cataggtcgc gtcaaactcg agtggcccaa ggtcctcgac agtcgtcggc tcaaccggct
     5461 cgcgtccgaa aagcatgcgc agcgcgacgg caatcaacac gatcccaaat agcgcggcca
     5521 gaagccgcgc atcgaccaga cccgcgatga acccaccaac gatgccaccc agggtcgtcg
     5581 ccacgaggag cgtcaggccg atcctgcgcg acacgaagcc ggcctccaag tagttcgctg
     5641 aagcggtcgc ggatatggcg atcacgccga gcaggctcac cgcaatggcg tggtggagcg
     5701 gcacacccag cgccacggac agcagcggga ccacgatgat cccgccgcca acgcccgcga
     5761 ttgcgccgaa cacgcccgcg cccagcgcgg cgagcagggc aagcagctcc agcgggatca
     5821 ctcggggcga tgttatggcc gctacgatcg ggccgtgccc cagacagcag cagttggccg
     5881 cggcggacca cttgttgggc tgcgtgtcct cgagctgtcg tcggtcctgg ccgggcctta
     5941 ttgcgcgatg gtgctggccg atctcggcgc cgacgtcatc aaggtcgagc cgctcgacgg
     6001 tgacgcgacg cgcggctacg gcccgccatg gatcaaccca gatgcaccgg ccagcgagcg
     6061 atccgcggtc tacttccaat cggtcaaccg caacaagcgc agcctgcgcc tggacctggc
     6121 atcggagggt ggtcgggagg ttgtccggcg actgatccgt aagtcggatg tgctggttga
     6181 gaacttccga cctggcggca tggatcggat cggcctcggc gatgcagccc tgaacgagct
     6241 caatccgaag ctcgtccgcc tgtcgatcac gggctacgga ccggagggcc cggattcaca
     6301 gaagccgggc tacgacttcg tggcgcaggc cgtgggcggg ctgatgagcg tgaccggatt
     6361 cccggacgca cagggcggcc agccgaccaa gctcggtgtg gcgatcacgg acatcgttac
     6421 cgggctgctc ggcgcggtcg gcgtgctcgc tgcgctgcgc agcggcgtgg gccagcgcat
     6481 cgacgtgtcg ctgctggagt cgactcttgc tcttctcatc aaccaggcgc acaacgcctt
     6541 tgccacgggc gagcagccgc cacgcctggg caacgcccat cccaacatcg tgccctacga
     6601 gacgttcgcg accgcggatg gcgagatcgt ggtcgccgtc ggcagcgagc gccaatggcc
     6661 caagctatgc ggggagctgg acttgcccga actcgccacg gacgagcgct tcgccgacaa
     6721 cggcgcgcgg gtgcgcaacc gtgatgtcct gcggcccatc ctcgccgccc gcctcgcgga
     6781 ggccgactcg acccactggc tgcaccggct cgatgaggcg ggcatcccct gcggccccgt
     6841 caactccgtc ctcgccgcct tcgagcaacc gcaggccgtc gcgcgcgaga tgcgcgtgga
     6901 ggtagagcat ccgaagctcg gacgggtgcc ccaggtcgga ttgccctaca agctgtcggc
     6961 tactcccgca tcaatccgca ccgcgccccc actcctgggc gagcactcga ccgaggtgct
     7021 tgtcgaaatt ggctactcga cagcggagat cgcggcgctc cgctccgggg gcgtcatcta
     7081 ggccgacgcg gttggcggcg ccgggcggcg acggaatgca acgtcgagcc cgaggattac
     7141 ccaagccaat ccgtgcagaa acacgcccgt gagagccacg gtgttccaaa tggtgggctc
     7201 ggtcgacgag gcaaggccca accggtcgat cccgaggatg cccagcaggc tgcccaccac
     7261 gagcgcggcc gcgccaatcc gagtcacctg gtggagcagg aatgtcacga cgccgaacca
     7321 ggcatagccc agccacgacg cgatcgatgc ccagaagaag acccagccaa atgttccgcc
     7381 aaagaccggc ggctcacgac cgattacgag caattccatc acgaacatcc acgtgccgaa
     7441 cacgagcgcc gggatggccc caaccaaggc caacccgggc gacctttggc gctggcgcaa
     7501 atgaatcgct gtgactactg tcaagccgcc aattccgaac acgaataggc ggatgtagtt
     7561 ggcgtcattg gggatgtcta ctacgaaggc agcgagcaga ctcgccccac cgagcaaacc
     7621 aaccagtccc agaagacgtg tcacaggcgt cggggcaaca gtcatcgagt gagccacggt
     7681 tcggttcctc ctcaggtgtt cgccaacggc agtggtgatc acgtcaatcg gcgcccgcag
     7741 ccacgccctg gtagcgccga cctcccgctt gtggtcagtg aagagctgaa ccatctgatc
     7801 ggagaactgc gcccgatagt cggccggata gagccgcagc aggccgcggt acaggcgatc
     7861 gttgcccccg gcggccgagg tcatcgtgcc tcgagcgccc agggcttggc tgcggcaaca
     7921 aggctctgga gtcgccgcgc ctctgcggcg acgacggtgc ggcccaagcc ggtcaagcgg
     7981 tagtagcgtc gcctctgatc gtccatctcc gggtccggcc gctcatcgga ttcctcgatg
     8041 aggccatcct cgagcagccg ccggatcgtg gtgtagaccc cgcccggtcc gagcctcgtc
     8101 gcgccgtcag tgatgcgcgc gacctcctgc atgatggcgt agccgtggcg ctcctccggc
     8161 ccgatcgcga gcatgatcaa gatcgccgtg cggctgacct ggggcggcgg cgcggcggcg
     8221 ctatgggctg tgctcatgcc ggtactatat caacggttga tacattgtca agtgatacag
     8281 ccggcgttgg taagatcagt gccggcgccg acatagctca gcggtagagc agctgtttcg
     8341 taaacagcag gtcctcggtt caaatccgag tgtcggctcc attccttcgc cgcaacgccg
     8401 gaaggagtgg atcgatgaag atcagactca tcctcgcggg cgcgatggcg ctcgcgatag
     8461 cggccccggc gctggccaag acgccataca cgtaccgcta cccggtcacg ccgggctaca
     8521 cgtcgggaat ggcaaacaca catggcggtg gctacccggc agcggacttg ataatcaaac
     8581 cgcgctgcgg gacggccgcg cgttcgcccg tggacggcgt ggtccttgaa gccgaccggc
     8641 ggaacctctg ggcgcagggc ttccggggcg gcgcttaccg gggcggcatt ttcgtgagca
     8701 tcctcggtga ggatggagtg cgctactaca tgagccactt cgcctcaatc cccgaagcca
     8761 ttaagcgcgg cgtgacggtc actgcgggtc agacggtagg catagtcggc aggaccgggc
     8821 gcgcggggtc ctgccacatt cacttcggga tcagcccgcc ctgtttcagg aagggcgatt
     8881 ggtggatcag gcgcggtgtc gtgtggccgc agccttacct ccggtcgtgg aagaaggctg
     8941 acattgctga cttgtcaccg caagccaggg tcgccagatg gctcagggcc aacggctgcc
     9001 cggcgaccga ggcggacctg ccatgagcgt caacaacaat ccggcaacag ttgcctttgc
     9061 cgacgccgac atagctcagc ggtagcgcag ctgtttcgta tacagcaggt cctcggttca
     9121 aatccgagtg tcggctccac tcctttctct atgccaaggt cggatcaggc gcgcgcgagc
     9181 tcgatcgcgg ccttcatgga aagccgacgg cccgactcgg tggtcagcgc ggcttcgtcg
     9241 tgcgcaaggg acgcgcgcgc ggtatcgacg aactcacgtc ccggcgtctc atccgagtag
     9301 acgttcacga tgccttcttg cgcggcgtag acctccgctg ccgcggcaat ctggacggcg
     9361 cgcgtagcat cctcagcagc cagggtcgag gccagcccga ccatcgatag cccgacgcca
     9421 cgcacgctgc cgatggcgtc gtaggcgttc accgaatcga agaagtagcc gcgagccgtg
     9481 gccgcatcac cgtgacgcaa atatgtccac gccatttcgc tcaagatgcg ggcctcttcg
     9541 gcgcgatcgc ctatcgactc gaaggcgtcg cgggcctgcg tgtacagctc caaagcgagt
     9601 cggaggtcgc cctgcgcggc ggcaatcgat gcgcgggcgc caagcgtcag gccaatgccc
     9661 tcgatgtcgc ccagcgtttg ctggatctgc agcgcctccg ccaaagagac ctgtgcagac
     9721 tcagaatccc cggaaacggc gcgaagaatc ccgtcgaacg cgagcgccat ccccaaacac
     9781 catgtgaagc cggcacgtcg gctgagcgtc acgcattcgc ccatcaaacg caaaccagcc
     9841 tgaaggtcta gacccagcag agcaacgccc tccaagagca tcgcgacggc ctgctcgcgc
     9901 tcggcgtcca ggtgggcggc cgccaagtac gcctcattgc actggtcggc tgcgagcttg
     9961 aattcgccca ggacccatga gctgagaccg gcgctgatca gggcgccggc gcgagcaacc
    10021 gtcggcaagc cagccttatc cgcgtcgagg aatgccagcg tgtagtcctt cgcggtcacg
    10081 ttcttgccac gaagatgcca atagaagtag aggtcgccgc acatctgcag gccggcctgc
    10141 acggcgcccg tgtcgccagt tcccgcggcc gccaggcggg tgtcaagggc cgacatgaga
    10201 ttgccttcgt cggccacgcc acgtgctaat gcgcctacct ggtccgtcga ctcaatgccg
    10261 tctcggatgg cgagcgcgac gtgtgcgtat cgctgcgcgt ggcgcatggc cgtggcggac
    10321 tcctcggcgg aggcgcggag ctgctcgcgc gcataatcgc cgatcgtctg gagcatgcgc
    10381 agccggccgg tgtgaccatc gacctggaca agcgccttgt cgacgagcga ctcaatcccg
    10441 tcgagagcgg actcgccagc gtccgcgcag atcgcctcga aatcgtccac cgcgcacccg
    10501 ccgacgaaga cggccatgcg gcggaatagc ttctggtcgg gctgagtcag cagggaatgg
    10561 ctccactcga ccgtcgcgcg caaagtctgt tgtcgctcgg gggctccacg cgggcctgag
    10621 gtgagcacgt tgagcgcatc accaagtcgc tcattcagcg cctgtggtga cagaatcctg
    10681 agacgcgccg cggccagctc caatgccagc gggagtccgt cgagccggcg gcagatatca
    10741 gcgatcgccc gggcgttctc gggcgtgacc tcaaaactgc tctttgccgc gcgtgctcgt
    10801 tcgatgaaca aggccgtcgc cgaatagtcc atgagtgaac ctgcgatgtc cttctggtcg
    10861 gcgtccggaa cgtccagcgt gggcagcgga tactcgcgct cggccgcaag tcgcaacggt
    10921 gtgcggctcg tcgtgactat tcgcagattc gggcagctct gggcgatgtg ggctacgtca
    10981 gcggcggccc cgacgatctg ttccatgttg tcaagcagca gcagcgcggt cttgtccccg
    11041 ataagcgcga caacgccctc gcgcagcgac cgttcttccg cctccttgac gtcgagtgcc
    11101 gccgcgagag ccggcacgaa gccaaccgga tcgagcacgt cggcaaggga cacgaacgag
    11161 acaccatcga ggaattcggg cgtcagcgtt cgaccgagct cgaccaggag gcgcgtcttc
    11221 ccgacgcccc caggcccggt gacggtgacg atccgctgcg cgcggacgag ctctacgatc
    11281 tgttgcagct cgcgcgaccg gccgacgagc gcggatgcag gctccgggag gttgctcggc
    11341 aagctcgcga accgctcgcc cttgccctcg agtgtctgcg catcgggcag cacgaggccg
    11401 tcgccggcaa ctgcgtacag ctcaacgggc tggccgatgc tcttcaactt gaagcggccc
    11461 aggctcacca ggtccacgtc gctccgattc ctgagctgtt cgcgagcagc atcggacagc
    11521 agcacgcccc cgggaaccgc aaacgattcg attcgagccg cgacattcac ggcgtcgccg
    11581 gtgagccgct cgcgctcaac gacgacctcg ccgacatgga tgccgatacg gacagatacg
    11641 tcggcggcgc tcagccggcg ctgagctgcc acagccgcgc ttactgctgc gagcgcgctc
    11701 ggaaacatgc tcatcgtccc gtcgcccagg cgctggacga tcgttccgcc gaacgcagcg
    11761 tgctcctgct cgacggcagc aatgtattga tcgcgcttca ggacggcgac ccgctcgtcc
    11821 gattgcatct gcgcgctata gccgaccatg tccgtgaaca tgaccgcgac gagcctgcgg
    11881 tcgagcgcgc tatccactga ccctcccacc ctctttccct agagacgcgc gccgagtcta
    11941 ggcccgcgac aacgcgcccg ccccagcttt cggcgaaatg ccgagtccca tcagtgaccg
    12001 ctagcctcag cggtagtgac aatcgacttg aacgactttc ctgagatcct cacgcccaga
    12061 ttgcggctgc gagcctggac ggatgcggat gccgaccagc tcgcggacat ctatgcggat
    12121 cccgagtcag tccgctacct gcgttactac gaccgcgcgg gcacgcgggc gcaggtccag
    12181 cggttccgcg accactggga cgagcacgga ttcggattgt gggcggtcga gcaccgcgaa
    12241 acgggacggt tcatcggccg gatcgggctg atgcaccacg acgactggag cgcgagcccg
    12301 cacgatgcgg aagtgggctg ggtgatcgcg cgcgaaatgt ggggtcaggg tctcgccagc
    12361 gagggtggcg ccgcagccct cgatttcggc aggaagaagg gcctcaatca cttcgtcagc
    12421 atcgcccacg tcgataacgc ggcgtcgcaa cgggtcatgg agaagctggg cctgacccgc
    12481 cagggcgtga ccgtgtggcg cgactcgccg gtcgtttggt tcgcgacgga ccggtgacgc
    12541 gagcatgacg cgaattgcgc tcgcgacctg tgccgccttg ccggatctcg atcccgacga
    12601 gcagctgctg ctcgagccgc tgcgtgccct gggtgtcgac gtgcaaccgg cagtgtggga
    12661 cgacgaggcc gtcgattggg cgtcgttcga cctcgtggta atccgctcga cgtgggacta
    12721 caccgcgcgg cgcgacgaat tcctggcctg ggcggaatcg gtgccgcgcc tcgtcaaccc
    12781 ggcacgggtc ctgcgctgga acacggacaa gcactacctg caggacctgg ccagcgcagg
    12841 cgtgcccgtg gttccgactg actggctcga gtccggcaat gcggtcatcc ttccggcaac
    12901 gggccggcac gtcctgaagc catccgtcgg cgctgggtcg gtcgacgccg cgctgttcga
    12961 ccttgctgtc gccgagcagg ccgggctggc gcgcgcccac gcggagcggt tggtcgccgc
    13021 cggccagacc gtgatggtcc agccgtacct ggagctgatc gaacgccacg gggagacggc
    13081 gctcatcttc tacggcggcc agttcagcca tgccgccgcg aagggcgcga tgctggtcag
    13141 ccagacagag atggtcgcgg gtctttacaa agcagagttg atcaagccac gggacgcgac
    13201 tgcggcggag atcgagattg cccgcgcggc gctgtccgcg gcgccgatcg ccgccgaaga
    13261 acttgcgtac gcccgcgtcg acttcatccc ggcggcggat ggcagcccgc tcgtcatcga
    13321 gctcgagctg acggagccat cgctcttcct gggccaggcg cccggctcag cggagcgtct
    13381 cgcgcgccac ctcctcgagc gcgcttccga agggacgggc gtagaggccg gacaaacgta
    13441 gaggccgggc gagcgcccca gcgctgctag cccggcccct tcaggagagg gtgtctcctg
    13501 accgttgcgc ggaggagagg gatctccgcg tgcaacgttc tggacgctac gttgggccag
    13561 ccggttccca agggcaatcc gccgtgtcac tgatttgggt actggccgcc atgtcggtat
    13621 ccggatggca cggctacggg cgtcctgctg cgcaacgagc aagagaccta aggcaaccag
    13681 tagccctgta gctgccagag gtcaccgaat aacccgttcg cagcctggcc tccgaagagg
    13741 agataggtcg aggcgatccc gcccacctgg atgaggctcg cgccagagcg tgcgctcggc
    13801 gaattgggaa gctcactcga aaaggcttcg accgaatccg acgagatctg gaccaggtcg
    13861 ctgagatagc cgccgtccaa cgatccgcca ccgaacgcga tcgcatcgaa gggcgaggta
    13921 ccgcgcgaac tactggcgag tgcgtatagc tgacggggcg gcgcacccga gtccgggccc
    13981 tctgtccagg tttgtgcact tgcgtcgtac gaccagatat cgcctaaggc gggcacaccc
    14041 gtggtctggc cgccatatag gacgagagtg tcttgagtcg accagtagca gtcgtgcagg
    14101 cagcgcttga ccggacgatc gccagcgggg gtctcgtcgg tccattcgcc ggtcgcgaag
    14161 tcataggcac gggtgtcgtc gaagcggccg tcgtcctggg tgaagccgtg gctgacccag
    14221 agccgcccgt ccggcccgag cgaggcgcaa gagccgtaac gggcatctgg cacggcgccg
    14281 agcgagggca gttgctgcca cgcgtcgacc ccagggtcgt aggcccagat gtcatcgaag
    14341 aagccgtcgc cctggccact ccaaaccaca agccccaccc ccgggaccat ggtggccgtg
    14401 tggccgaagc gcggggccgg ggggggtccc gcgggctgga gctcgctcca cgagtacgtt
    14461 tcgagatcaa atgaccagag gtcgttggac ggcccgtcag cagtccggcc gccgaacagg
    14521 tacgcaacgg gcctgtcgcc ttcagcgcca accgcccagc cagctgtcca cgtgtggtca
    14581 gtgcgggctg acggaccgga agcgacctcg gtcaactccc gccaggtggt tgcgtcctcc
    14641 gcgatgggtg tgatggtagg tgttggcacg accggggttg taggtccgga cgtgggcgtg
    14701 gc
//
LOCUS       DATBRX010000094         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1928810, whole genome shotgun sequence.
ACCESSION   DATBRX010000094 DATBRX010000000
VERSION     DATBRX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928810"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VIF08_03430"
     CDS             complement(<1..690)
                     /locus_tag="VIF08_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8135066.1"
                     /translation="MKTSYDVIVVGGGHNGLVCAAYVARAGRSVLVLERRDVAGGVLA
                     APELMPGVPAPGPVNSVGRLDARVAKDLWLDTHGVKLVTSQARLTSIADDGRALTLWA
                     DPTRTAADLRLVLRTDGAAYARLDSDLRAMARFGARIAAAAPPDVARLRASDLRTGMG
                     LLRGYRGMGRRLAHDFLRVLPMPVGDVVADYVDDDQLRAAIAWRGVRYTSLAPSDAGS
                     AQVLLADMVGGD"
     gene            complement(687..2294)
                     /locus_tag="VIF08_03435"
     CDS             complement(687..2294)
                     /locus_tag="VIF08_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8135067.1"
                     /translation="MAGGGRGERRYDAIVIGGGHNGLTSAAYLARAGLKTLVLERRHV
                     LGGAAVTEEIVPGFRFSVASYVVSLLRPEIVRELELPRHGLSILPLDGTFTPLDNDYL
                     WRTDDHGATRRELRRWSLRDAEAYEEYALLMVEMARFIKPILSIVPPDPTHLDPREFL
                     PLAGLAQAFRKLPRVQQQTFIRLMTMSAAEFLDRWFETDPLKATMSASGIIGTFLGVR
                     SPGTAYVLLHHYIGEVDGAFRAWGIPKGGTGGVSAAIASAAREQGAEIRTEAPVARIL
                     TRDGRATGVVLESGEEIEAGVVLSSLDANQTFLKLIEPGTLEPEFEREVRRYRYRGSS
                     GKVNLALDGLPDFTCLPGFGDHLRGAISFSPSVDYMEQAYIDAREGRFSRRPYIDMII
                     PTLTDPTMAPPGKHIISCFVQYAPYELDPALGGWDAQREAFGDTVIDTIAERAPNIRS
                     LIVGRQVLTPLDMERTLGLTQGNIFQGELTLSQLFFSRPVPGWARYKTPLRSLWMCGS
                     ATHPGGGIMGAPGRIAALEVLKSVGRR"
BASE COUNT          355 a          808 c          811 g          327 t
ORIGIN      
        1 atcgccgccg accatgtccg cgagcagcac ctgcgccgag ccggcgtcgg acggcgccag
       61 cgacgtgtag cggacgccgc gccaggcgat cgcagcgcgg agctggtcgt cgtccacgta
      121 atcggcgacg acatcgccca cgggcatggg taggacgcgc aggaagtcgt gcgccaggcg
      181 gcggcccata ccgcgatagc cgcgcagcag gcccatgccg gtgcgcaggt cggacgcgcg
      241 caacctggcg acgtcaggtg gcgccgcggc cgcgatccgt gcaccgaacc tggccatcgc
      301 gcgcaggtcg ctatccagcc gcgcgtaggc cgcgccgtcg gtgcgcaaca ccaggcgcag
      361 gtccgcggcg gttcgcgtgg gatcggccca cagggtcagc gcccgaccgt cgtcggcgat
      421 gctcgtcagc cgggcctgcg aggtgaccag cttcacgccg tgcgtgtcga gccacaggtc
      481 cttggcaacg cgcgcgtcca gccgcccgac agagttgacc gggcccggcg cgggcacgcc
      541 gggcatcagc tcgggcgcgg ccagcactcc gcccgccaca tctcgtcgtt ccagcaccag
      601 cacactgcgt ccggcgcgcg cgacgtacgc ggcgcagacc aggccgttgt ggccgccgcc
      661 gacgacgatc acgtcgtagc tggtcttcat cggcggccca cgctcttgag gacttcgagc
      721 gccgcgatcc tgcccggcgc gcccatgatg ccgccgccgg ggtgcgtcgc cgatccgcac
      781 atccacaggc tccgcagcgg cgtcttgtag cgcgcccagc ccgggaccgg gcgcgagaag
      841 aacagctgcg acagtgtcag ctcgccctga aagatgttgc cctgggtcag gcccagcgtc
      901 cgctccatgt cgagcggcgt cagcacctgg cggccaacga tcagcgagcg gatgttgggc
      961 gcgcgctcgg cgatggtgtc gatgaccgtg tcgccgaacg cctcgcgttg cgcgtcccag
     1021 ccaccgagcg ccggatcgag ctcgtacggc gcgtactgga cgaagcacga aatgatgtgc
     1081 ttgcccggcg gcgccatggt cgggtcggtg agggtgggga tgatcatgtc gatgtagggc
     1141 cggcgactga atcggccctc ccgggcgtcg atgtaggcct gctccatgta gtcgacgctc
     1201 ggcgagaagc tgatcgcgcc gcgcaggtga tcgccaaagc caggcaggca ggtgaagtcg
     1261 ggcagcccgt caagcgccag gttcactttc cccgacgatc cgcggtagcg atagcggcgc
     1321 acctcgcgtt cgaactccgg ctcgagcgtg cccggctcga tgagcttcag gaacgtctgg
     1381 ttggcatcga ggctcgagag gacgacgccc gcctcgatct cctcgccgct ttccagcacg
     1441 acgcccgtgg cgcgaccgtc gcgcgtcagg attcgtgcga cgggcgcctc tgtccggatc
     1501 tcagcgccct gctcgcgcgc cgcggacgcg atggccgcgc tgacgccgcc ggtgccaccc
     1561 ttggggatgc cccacgcccg gaaggcgccg tcgacctcgc cgatgtagtg gtgcaggagc
     1621 acgtaggcgg tgcccggtga gcgcacgccc aggaacgtgc cgatgatgcc cgacgcggac
     1681 atcgtggcct tcaatggatc ggtctcgaac cagcggtcga ggaactcggc cgcgctcatc
     1741 gtcatgagcc tgatgaacgt ctgttgctga acgcgtggca gcttgcggaa cgcctgggcg
     1801 aggccagcga gcggcaggaa ctcccgcggg tcgaggtggg tcgggtcggg cgggacgatc
     1861 gagaggatgg gcttgatgaa gcgcgccatc tcgaccatca gcagcgcgta ctcctcgtag
     1921 gcctccgcgt cgcgcagcga ccagcggcgc aactcgcgcc gggtcgcgcc gtggtcgtcg
     1981 gtccgccaca ggtagtcgtt atcgagcggc gtgaacgtgc cgtcgagcgg caggatcgac
     2041 aggccgtggc gcggcagctc cagctcgcgc acgatctcgg ggcgcaacag gctcacgaca
     2101 tacgacgcga cgctgaagcg gaagccgggc acgatctcct cggtcaccgc cgcgccaccc
     2161 aggacgtggc gtcgctcgag gacgagcgtc ttcaatccag cgcgggcgag atacgcggcg
     2221 gacgtcaggc cgttgtgccc gccaccaatg acgatcgcgt cgtaccgacg ctcccctcgc
     2281 cctcctcctg ccacacgcta a
//
LOCUS       DATBRX010000095         5295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3605012, whole genome shotgun sequence.
ACCESSION   DATBRX010000095 DATBRX010000000
VERSION     DATBRX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3605012"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VIF08_03440"
     CDS             complement(<1..806)
                     /locus_tag="VIF08_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135068.1"
                     /translation="MALDEQVLGQFLGDDSFPIEWASETEKKLFWVFDDLHCPHPLSP
                     MFEEIGGWWLSCDHMFRRFGTPFASDWIIKNVNGYLYTAAIPAEAGVDVSSEEYGARY
                     AARVPLDAGYPARIGKYLDAVLPVYGLHFADWWRDRLVPEMQRNFEYLEGRLDDAPNL
                     SLMELATVWEDAIDIHDRHWKIHWMLNFAQLSATLDLRAAMEKVHGKIDEALLGRLQN
                     SASDRNWDSIEALWKAKEEVKADPELSEAFSAGGAEAIIARLNASDRGRR"
     gene            916..1959
                     /locus_tag="VIF08_03445"
     CDS             916..1959
                     /locus_tag="VIF08_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEY8135069.1"
                     /translation="MTQRTPTRYWNDSCAVAELAHAVERGATGATSNPSIVMEVLGKE
                     SEHWIPRIRELATDHPDWSEVELTWAIAEEMAARGAAVLEGVGRLSMQVNPAAHRSPA
                     LMLEQARRFAALAPNMQVKFPNTAPGLVAIEEATALGININATVSFSVPQAIAAAEAV
                     ERGLTRFAAGGGDADSMTPVITLMIGRMDDWVKVLVARDRIALREDAAEWAGIAVFKR
                     AYRLFLERGYRARLLAAAYRNTLHWTELVGGEVTLTMPFAWQQKFDASGIEPEPRMDV
                     PVDPELVADLLEKIPDFRRAYEPDGMTADKFESFGPSARTLRAFIKSYHDLQGAIRDV
                     YLPDPDVPLASGA"
     gene            complement(2104..3279)
                     /gene="hemW"
                     /locus_tag="VIF08_03450"
     CDS             complement(2104..3279)
                     /gene="hemW"
                     /locus_tag="VIF08_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY8135070.1"
                     /translation="MTQPVALYVHFPFCVSICPYCDFVVYGGKDARGPSNRIAYLVDA
                     LEVEIGLRAVPGANLGSVYFGGGTPSLMSATQVERLLAAADDAFGLQSDAEITIEANP
                     GGGERGDLPGFRAAGVNRLSIGAQSFDPTELKRLGRRHAPTDISATVRAARQAGFANI
                     SLDLLYDVPGQTIDSWRDTLAGTLALEPDHISAYALTLDDPDLEGITGPTGDHLPLRP
                     GARNWRTQASSEQDADRAAAMYEMADDAFATAGLHWYELSNWSLLGRESRHNLTYWRG
                     QAWEAIGPGAHAFDGELTRRWNAARLDAYLAALSAGHLPPGDSVTVDAATAQAERAML
                     ALRTGSGLIVGEMPGIETVIAWGTEQSLLESDESVVRLTRRGRLLSNELFARLVPAA"
     gene            complement(3276..4055)
                     /locus_tag="VIF08_03455"
     CDS             complement(3276..4055)
                     /locus_tag="VIF08_03455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY8135071.1"
                     /translation="MTLDFENLMTVAALGLLIVLRVDARRFGAADFDDETAVDGVRVW
                     ARRLTWYGLGIVLVLAAYFLFPRPEAGLHLRLGEDRVFAVMAGLTLAMLGIGAAVLYA
                     WWRFGEVKLPDARAYPVGLLNSMATAIIDEAAFRGVLLGLLLLSNWPPVYAVAFQAVA
                     YALATRLGGAGRPRSLLILWLVVGLLNGWLTYLTGGIGAAVLTHALTRFAIFTATGHA
                     GQVTPSAVSEEEAHELAETAGDAGLDVVPDPEPGGYLRPIE"
     gene            complement(4063..>5295)
                     /gene="lepA"
                     /locus_tag="VIF08_03460"
     CDS             complement(4063..>5295)
                     /gene="lepA"
                     /locus_tag="VIF08_03460"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY8135072.1"
                     /translation="LRGLIFDSHYDPYKGVVAYVRLFEGRLADHESVRLMASGAQSEI
                     LELGVFRPQQVAVDALGPGEVGYVATGLKSVRDCQVGDTMTTSARAAVTPLPGYKQAK
                     ALVFAGLYPIVGEDFPDLREALEKLHLNDASISFEPETSIALGTGFRVGFLGLLHMEI
                     VQERLEREFNLDLIASAPSVEYRVRLTAGRGEVVVDNPAHLPPLGEIDAISEPWVKAS
                     IITPSSYIGTLTELATGRRGAFEGIEYLDPERVLLTFELPLAEVIVDFFDQLKSRSQG
                     YASLDYEEIGYREGELVRLDVLVNGEPVDALSMIVHRSNSHSLGRELVGRLKKLIPRQ
                     MFEVPVQAAIGSNVIARETISAMRKNVLAKCYGGDITRKRKLLEKQKAGKARMKRVGS
                     VEIPQEAFLAVLRSTVEA"
BASE COUNT          859 a         1781 c         1795 g          860 t
ORIGIN      
        1 aagcgccggc cccgatcgga cgcgttgagc ctggcgatga tggcctcagc accaccggcg
       61 gagaaagcct ccgacagctc cgggtcggcc ttgacctctt ccttcgcctt ccacagggcc
      121 tcgatggagt cccagttgcg atcgctcgca ctgttctgca gacggcccag cagcgcctcg
      181 tcgatcttgc cgtgcacctt ttccatcgcc gctcttagat cgagcgtcgc ggagagctgc
      241 gcgaagttca gcatccagtg gatcttccag tgccgatcgt ggatgtcgat cgcgtcctcc
      301 cagacggtcg cgagctccat cagcgacagg ttgggcgcgt catcgaggcg accctcgagg
      361 tactcgaagt tgcgttgcat ctccggcacg aggcggtcgc gccaccagtc ggcgaagtgc
      421 aggccgtaca ccggcagcac cgcgtccagg tacttgccga tcctggcggg gtagcccgcg
      481 tcgagcggca cccgggcggc gtatcgggcg ccgtactcct cgctggacac gtccacgccg
      541 gcctcggcgg ggatcgccgc ggtgtagagg tagccgttga cgttcttgat gatccagtcg
      601 ctcgcgaagg gcgtgccaaa gcggcggaac atgtggtcgc acgacagcca ccagccgccg
      661 atctcctcga acatggggct cagcgggtgc gggcagtgca ggtcgtcgaa gacccagaag
      721 agcttcttct cggtttccga ggcccactcg atggggaacg agtcgtcgcc caggaactgg
      781 ccgaggacct gttcgtcgag cgccaccttt tgccctcctc ctgccaagtc cgtttcaacg
      841 gcaattcggc tcttgccgtc ttccagtgtt catgcaagcg tacccggtgt gactgtcgat
      901 tcggcccttt tgcgcatgac acagcgcacc cccacccgct attggaacga ctcgtgcgcg
      961 gtcgctgagc tggcgcacgc cgtggagcgc ggcgcgaccg gcgccacgtc caacccgtcg
     1021 atcgtgatgg aggttctcgg gaaggagtcg gagcactgga ttccgcggat acgggagctg
     1081 gccaccgacc accccgattg gagcgaggtc gagctgacgt gggcgatcgc cgaggagatg
     1141 gcggctcgcg gcgccgcggt gctcgagggc gtgggtcgac tatcaatgca ggtcaatccg
     1201 gctgcgcatc gctcaccggc gctgatgctg gaacaggcac gccggttcgc cgcgctcgcg
     1261 ccgaacatgc aggtcaaatt tccgaacacc gcgcctggcc tggtcgcgat cgaggaggcg
     1321 acggcgctgg gcatcaacat caatgcaacc gtctcgtttt ccgtgcccca ggcgatcgcg
     1381 gcggcagagg ccgtcgagcg cggcttgacg cggttcgcgg cgggtggcgg cgacgccgac
     1441 tcgatgacgc cggtgatcac gctgatgatc ggccgcatgg acgactgggt gaaggtgctc
     1501 gtcgcgcggg acaggatcgc gttgcgcgag gacgcggcgg agtgggccgg gatcgccgtg
     1561 ttcaagcgcg cgtaccggtt gttcctcgaa cgcggctacc gcgcccggtt gctcgccgcc
     1621 gcctaccgca acacgctgca ctggacggag ctcgtcggcg gcgaggtgac cctcacgatg
     1681 ccgttcgcat ggcagcagaa gttcgacgcg agcggaatcg agcctgagcc gcgaatggac
     1741 gtgccggtcg atcccgagct ggttgcggac ctcctggaga agatccccga cttccggcgc
     1801 gcctacgaac ctgacggcat gaccgctgac aaattcgagt cgttcggccc gtccgcgcgc
     1861 acgctgcgcg cgttcatcaa gtcctaccac gacctccagg gcgcaatccg agacgtctac
     1921 ctgcccgacc cagacgtgcc attggcgagc ggcgcatgag cgcgctccga gtgctcctgt
     1981 tgatcgccgt cacgttcgtc gcggtcctgg tgatcttcta tctacttggc atcgggttcg
     2041 tggcctagcg cacatcgcgc acaagttccc actcgtcgac cgtcacctgc cgaacggcgg
     2101 cgctcatgcc gctgggacta ggcgcgcgaa gagctcgttg ctgaggaggc ggccacggcg
     2161 ggttagtcgg acgaccgatt cgtcggactc caacaggctc tgctccgttc cccaggcgat
     2221 gacagtttcg attccgggca tctcaccgac tatcagtccg ctgccggtac gaagggcgag
     2281 cattgccctc tccgcctggg ccgtcgcggc gtcgaccgtc accgaatcgc ctggcggcag
     2341 gtggccggcc gacagcgcgg ccagataggc gtcgaggcgg gcggcgttcc agcggcgggt
     2401 cagctcgccg tcaaaggcgt gtgcccccgg accgatcgcc tcccaagcct gaccgcgcca
     2461 gtacgtgagg ttgtggcggc tctcgcgacc gaggagtgac cagttcgaga gctcgtacca
     2521 gtgcaggccg gccgtggcga aggcatcgtc ggccatctcg tacatcgcgg cggcccgatc
     2581 cgcgtcctgt tcggagcttg cctgggtgcg ccagttgcgc gcgcccggtc gcagcgggag
     2641 gtggtcaccg gtcgggccgg tgatgccttc caggtctggg tcgtcgagcg tcagggcgta
     2701 ggccgagatg tgatccggct ccagggcgag tgtgccagcc aaggtgtcac gccacgagtc
     2761 gatggtctgt ccgggcacgt cgtacaggag gtcgaggctg atgttggcga agcctgcttg
     2821 gcgggcagcg cgcaccgtgg cgctgatgtc tgtgggcgcg tggcgccggc ccaggcgctt
     2881 cagctcggtc gggtcgaaac tctgcgcgcc aatgctcagt cggttcacgc ctgccgcgcg
     2941 gaatccgggg aggtcgccgc gctcgccgcc gcccgggttg gcttcgatcg ttatctcggc
     3001 atctgactgg aggccgaacg cgtcatccgc ggcggcaagc agccgctcaa cttgggtcgc
     3061 gctcataagt gacggcgtgc caccgccgaa gtagacgctg cccaagtttg cgcccggtac
     3121 ggcccgcagg ccgatctcga cctcgagtgc gtcgaccaga tacgcgatgc ggttcgacgg
     3181 gccgcgcgcg tccttgccgc cgtagacgac gaagtcgcag tacgggcaga tcgagacgca
     3241 gaacgggaag tggacgtaca gggctaccgg ctgggtcatt cgatcggccg taggtagcca
     3301 cccggctcag ggtccgggac gacatcgaga cccgcgtcac cggccgtctc ggccaactcg
     3361 tgcgcctcct cctcggaaac ggcggagggg gtgacctggc cggcgtggcc ggtcgccgtg
     3421 aagatcgcga agcgtgtcag ggcatgcgtc agcacggccg cgccgatgcc gccggtcagg
     3481 tacgtcagcc agccgttcag cagacccacg accagccaca ggatgagcag gctgcgcggc
     3541 cggcccgcgc caccaagtcg cgtcgcgagt gcgtaggcga ccgcctggaa cgcgaccgcg
     3601 tagaccggcg gccaattcga gagcagcagc aggccaagca ggacaccgcg gaaggcggcc
     3661 tcatcgatga tcgcggtggc catcgagttg agcagcccca ccgggtaggc gcgggcgtcg
     3721 ggcagcttca cctcgccgaa gcgccaccac gcatagagca cggccgcgcc aatgccgagc
     3781 atcgccagcg tcagcccggc catcaccgcg aagacgcggt cctcgcccag gcgtagatgg
     3841 agccctgcct cgggccgcgg gaacaggaaa taggccgcga gcaccaggac gatgcccagg
     3901 ccataccagg tgaggcggcg cgcccacacg cgcacgccat caaccgcggt ctcgtcgtcg
     3961 aagtccgcgg cgccaaagcg ccgtgcgtcg acgcgcagca cgatcagcag ccccagtgcc
     4021 gcaaccgtca tcaggttttc gaagtcgagc gtcatgcgcg acctacgcct cgaccgtcga
     4081 gcgcaacacc gcgaggaacg cctcctgcgg gatctcgacg ctgccgacac gtttcatgcg
     4141 cgccttgccg gccttctgct tctcgagcag cttgcgcttg cgcgtgatgt cgccgccgta
     4201 gcacttggcg aggacgttct tgcgcatcgc gctgatggtc tcgcgggcaa tgacgttcga
     4261 gccgatcgcc gcctggacag gcacttcgaa catctggcgc gggatcagct tcttgagtcg
     4321 ccccacgagc tcgcgcccga gactgtgcga gttggagcgg tgcacgatca tcgacagcgc
     4381 atccactggc tcgccgttca ccaggacatc gagccggacg agctcgccct cgcgatagcc
     4441 gatctcctcg taatcgagcg acgcgtagcc ctgcgagcgg ctcttgagct ggtcgaagaa
     4501 gtcgacgatc acctccgcca ggggcagctc gaaggtcagc agcacccgct ccggatcgag
     4561 atactcgatg ccttcgaagg cgccgcgccg cccggttgca agctcagtaa gcgtcccgat
     4621 gtacgagctc ggggtgatga tgctggcctt gacccacggc tcgctgatcg cgtcgatctc
     4681 gcccagcggc ggcaggtgcg ccgggttatc gaccaccact tcgccgcggc cggcggtgag
     4741 tcgcacccgg tactcgacgg acggcgccga cgcgatcagg tccaggttga actcgcgctc
     4801 gagccgctcc tggacgatct ccatgtggag cagccccagg aagccgacgc ggaacccggt
     4861 gcccagcgca atcgaggtct ccggctcgaa cgagatcgag gcgtcgttga ggtggagctt
     4921 ctcgagcgcc tcgcgcaggt ccgggaagtc ctcgcccacg atcgggtaga ggcccgcgaa
     4981 tacgagcgcc ttcgcctgct tgtagcccgg cagcggcgtg acggcggcac gagcggaggt
     5041 ggtcatggtg tcgccgacct ggcagtcgcg cacgctcttg agccccgtgg cgacatagcc
     5101 gacttcgccg ggacccagcg catcgacggc cacctgctgt ggtcggaaga cccccagctc
     5161 caggatctcc gactgggcgc ccgaggccat cagtcggacc gattcgtggt cggccagcct
     5221 gccctcgaag agtcggacgt aggcgaccac gcccttgtag gggtcgtagt gcgaatcgaa
     5281 gatcagcccg cgcag
//
LOCUS       DATBRX010000096         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1259647, whole genome shotgun sequence.
ACCESSION   DATBRX010000096 DATBRX010000000
VERSION     DATBRX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259647"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..660)
                     /gene="smpB"
                     /locus_tag="VIF08_03465"
     CDS             complement(196..660)
                     /gene="smpB"
                     /locus_tag="VIF08_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY8135073.1"
                     /translation="MSETVALNRKARHNYLITDTFEAGIVLTGTEIKSIRAGKANLSD
                     AYARVERREAWLVGAHIAPFEQGNRYNHEPRRDRKLLLHRTEIDSLMGKAAAKGLTVV
                     PLRLYISDKGRAKVELGLGKGKQLHDRRRDIADRQSKRDVERELADATRGRR"
     gene            complement(852..2240)
                     /locus_tag="VIF08_03470"
     CDS             complement(852..2240)
                     /locus_tag="VIF08_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8135074.1"
                     /translation="MNDQMSAVPSSNLPDTPPDRAQVAPHLDPQLEQRSRDRRASTLL
                     ALLVVAVLGGAGIFVSGFTLGRLAGATPGTTDANQDLFRPFWDAYTDVSSNYVGQIDP
                     HFLVEGAIKGMFNALDDPFSQYLTEEEYRASLGGISGEFEGIGVEMSTQDAAGTPCDT
                     ISDTCRLVITRVIRRSPAITAGLRDGDVVTAVDGQVTRGRTLGEVVPTIRGPKGTAVT
                     LTIVRGTETLDVPVTRDVIQREDVRSELLADGRVGYLQVSGFSSASAQDLHDMLTQLV
                     QTDHVAGLILDLRDDPGGYVDAAHKIANEFSAADPLYWEQSATGEPEPKHPNPGGAAT
                     DTAIPLVVLVNGGTASASEIVAAALQGNGRALLIGSLTYGKGTIQEFKELPGAGGYRL
                     SVRKWLTPDQTWIHGVGLRPDIAVLPPADEQAPNDVVLQRAVQVVLDQIAGKPVTTDP
                     PPTPSLAPTPSA"
     gene            complement(2279..3145)
                     /locus_tag="VIF08_03475"
     CDS             complement(2279..3145)
                     /locus_tag="VIF08_03475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135075.1"
                     /translation="MVDLRPIDPADLDVLGEVFYEADDELNVRRGLPTTPRNPMPLLR
                     LFDHIIKESPQRGWLAERDGRALGFGIAAQRVDMTFLSFLFVRPDAQASGLGRELLVR
                     AMSGSTYRAVCIGAIQPISAALYAQYGMVPRVPLFMFSGKPNTTLRALPEGLEIGPVS
                     IDDVAQLDHEVTGVTRPGDHAAWERWDRQRLGLYEAGRLVGYGYVQSVGRLGPVVVRD
                     AGHLLPFVGALIGRLPDVETWLLNVPGAAAETFETLLKAGLRLEGPPVIYCATELRID
                     HSRYLPAGFALP"
     gene            complement(3145..4257)
                     /gene="prfB"
                     /locus_tag="VIF08_03480"
     CDS             complement(3145..4257)
                     /gene="prfB"
                     /locus_tag="VIF08_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY8135076.1"
                     /translation="MPCASSSSASTRPRGGFDLAAKEKRVDELDARASVEGFWSDQRA
                     AQKVLREADSLREEVGTWRALEKRADDLLELAQLAESGADPDLAAELDSEFGAVSAEY
                     EGLRTSLLFSGDYDADNAILTITAGAGGTEATDWAEMLLRMYLRWAERHRFHTEILDQ
                     LEGEAAGLKSVTVDIRGRNAYGHLRAERGVHRLVRISPYDSQKRRHTTFALVEVVPEV
                     EDDTEVGDIPEDELRIETFRSQGAGGQHVNKTDSAVRLTHIPTGITAQSQNERSQHQN
                     RESAMKILKARLLEQRIAEREKELSALRGEHVEAGWGNQIRSYVLHPYQMVKDHRTSY
                     ETSATSDVLDGDLDAFMKAELERLATTVPVAAAGDR"
     gene            complement(4655..5881)
                     /locus_tag="VIF08_03485"
     CDS             complement(4655..5881)
                     /locus_tag="VIF08_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135077.1"
                     /translation="MDDIAREYLLIALAIDEIEHGIVDSYYGPPELREQAKSGNDGAA
                     ALAGRAAALRRRLNDETDEQQRALWLDRQLLGLETIARRLAGETLPYLVEVEHCFDAA
                     PEPTPPEDYARTRAELEELLPGDGDLRDRLAQRDERLTVPADRLPAIIDWVAAELRSA
                     AAAIWPLPDGEELSFSMVRDEPWAAYNWYDGNLKSRVEVNTDLPMRAHQVALTLAHET
                     FPGHHMEHAWKEARLYRERGYGEASAMLINTPESYISEGLAEVGAWLLNNETRFQEML
                     LGVTERAGIALTTDDAQREWHTSNALHRLRGAGGDAALQLHVAKRSRDDVVRFLEQDA
                     LSTHERALKVLEFIDHPLWRTYVFCYAGGERLLSHWVESAGDKEAQHARFSRLLTEQI
                     TPSGIAAESMQIKIPY"
BASE COUNT          913 a         2010 c         2052 g         1057 t
ORIGIN      
        1 tccggggcca cctgagccgc aggctggcca atttgggtcg gttggacctg ataggtccac
       61 cgttcggccc ggtgagccgc atcttgtgtc aaccaggtcg cgactggtcc aactggcgca
      121 atttgtgggc cgattcgtga acggtggacc gaatgcgtcc aaacgacgca ttcatgccgg
      181 ccggccactt aggggtcagc ggcggccgcg ggtggcgtct gcgagctcgc gctcaacgtc
      241 gcgcttcgac tgccggtcgg cgatgtcgcg ccggcggtcg tggagctgct tgcccttgcc
      301 caagccgagc tcgaccttgg cgcggccctt gtcgctgatg tacaggcgca gcggcacgac
      361 cgtcaggccc ttggccgcgg ccttgcccat cagcgagtca atctccgtcc gatggagcag
      421 cagcttgcgg tcgcgccgcg gctcgtggtt gtagcggttg ccctgctcga acggcgcgat
      481 atgggcaccg acgagccacg cctcgcggcg ctcgacacgc gcgtaggcgt cggacaggtt
      541 ggccttgccg gcgcggatgc tcttgatctc cgtgccggtc aggacgatgc ctgcctcgaa
      601 cgtatcggtg atgaggtagt tgtggcgcgc cttgcggttg agcgccacgg tctctgacac
      661 tcgagaagtc ctctggtacg aactagtgga attgtcgccg gacacacgaa cgccggccca
      721 aggcgggccg gcgtctcgtg atcttgccta caccatctcc ggtgtcagct caggcgagca
      781 tcccccggga gatggtgcag ctagaacgat ctaacactgc acatcacctc ctttcgttcc
      841 caaacaatct atcacgcgct cggggtgggt gccaatgagg gtgtaggcgg cgggtcggtc
      901 gtcacgggct tgccggcgat ctggtcgagc accacctgca ctgccctctg gagcactacg
      961 tcgttaggcg cctgctcgtc ggccggcggc agcacggcga tatccggccg caggccgacg
     1021 ccatggatcc acgtctggtc cggtgtgagc cacttgcgca cgctgaggcg gtagccgccc
     1081 gcgccgggca gctccttgaa ctcctggatg gtgcccttgc cgtaggtcag cgagccgatc
     1141 agcagcgcgc ggccgttgcc ctggagcgcc gccgcgacga tctcgctggc gctcgccgtg
     1201 ccgccgttca ccaggaccac gagcgggatc gcagtgtccg tcgccgcgcc acccgggttg
     1261 ggatgcttcg gctccggctc acccgtggcg ctctgctccc agtagagcgg atcagcggcg
     1321 ctgaactcgt tcgcgatctt atgcgccgcg tcgacgtaac cgcccgggtc gtcgcgcaga
     1381 tcgaggatca gtcccgccac gtggtcggtc tggacgagct gcgtgagcat gtcgtgcagg
     1441 tcctgtgcgg acgcggacga gaagccgctg acctgcaggt agcccacccg gccatcggcg
     1501 agcagctcgc tgcgcacgtc ctcgcgctgg atcacgtcgc gcgtgaccgg tacgtcgagc
     1561 gtttccgtgc cgcgcacgat ggtcagcgtc acagccgtgc ccttggggcc gcggatggtg
     1621 ggcacgacct ccccgagcgt cctaccccgg gtgacctggc cgtccaccgc ggtgacgacg
     1681 tcgccatcac ggagcccggc cgtgattgcc ggggagcggc ggatcacgcg cgtgatcacc
     1741 agccggcacg tgtccgagat tgtgtcgcac ggcgtgccgg ccgcgtcctg ggtggacatc
     1801 tcgacgccga tgccctcgaa ctcgcccgag atgccgccca gcgaggcgcg gtactcctcc
     1861 tcggtcaggt actgcgagaa cggatcgtcg agcgcgttga acatgccctt gatcgcgcct
     1921 tcgaccagga agtgcggatc gatctggccg acgtagttcg agctgacgtc ggtgtacgcg
     1981 tcccagaagg gccggaacag gtcctggttg gcatcggtcg tgccgggcgt ggcaccggcg
     2041 agccgaccca gcgtgaaacc ggacacgaag attccagccc cacccaggac cgcgacgacg
     2101 agcagcgcca gcagcgttga tgcgcggcga tcccgcgatc gttgctccag ctgcggatcg
     2161 aggtgcggcg cgacctgcgc gcggtccgga ggcgtatcgg gcaggttcga ggaaggcaca
     2221 gcagacatct gatcgttcac gggagcgcca gtctatgtgc cgtggctgtg aaccaggatc
     2281 agggcagggc gaagcctgcg ggcaggtagc ggctgtggtc gatgcgcagc tcggtcgcgc
     2341 aatagatgac cggcggcccc tccaggcgca ggccggcctt gagcagcgtc tcgaacgtct
     2401 ccgccgcggc cccggggacg ttgagcagcc acgtctcgac atcgggcagg cgacctatga
     2461 gcgcacccac aaacggcagc aggtgccccg cgtcacgcac gacgaccggt cccaggcgac
     2521 cgaccgactg gacgtagccg tagccgacca gcctgccggc ctcgtacaac cccaggcgct
     2581 ggcgatccca cctttcccat gccgcgtggt cgcccggccg ggtgacgccg gtcacctcgt
     2641 ggtcgagctg ggccacgtca tcaatgctga ccggcccgat ttccagacct tccggcaagg
     2701 cgcgcagagt ggtgtttggc ttgccgctga acatgaacag cgggacgcgc ggcaccatcc
     2761 cgtactgcgc gtaaagggcc gcggagatgg gctggatcgc gccgatgcac accgcgcggt
     2821 acgtgctgcc ggacatggcg cgcaccagca gctcccggcc aaggccgctc gcctgggcat
     2881 ccggacgcac gaacaggaac gagaggaacg tcatgtccac ccgttgcgca gcgatgccaa
     2941 agcccagcgc ccgtccgtcg cgttcggcca gccagccgcg ttgcggcgat tccttgatga
     3001 tgtgatcgaa gaggcgcagc aggggcatgg ggttgcgcgg cgtcgtgggc aggccgcggc
     3061 gcacgttcaa ctcgtcgtcg gcctcgtaga agacctcgcc caggacgtcc aagtcggccg
     3121 gatcgatcgg ccggaggtcg accatcagcg gtcgccagcg gccgcgaccg ggacagttgt
     3181 tgcgagtctt tcgagctcgg ccttcatgaa cgcgtcgaga tcgccgtcga gcacgtccga
     3241 cgtggcgctg gtctcgtagc tcgtgcggtg gtccttgacc atctggtagg gatgcagcac
     3301 gtacgagcgg atctggttgc cccagccggc ctccacgtgc tcgccgcgca gcgccgatag
     3361 ctccttctcg cgttctgcga tgcgctgctc gagcaatcgg gctttgagaa tcttcattgc
     3421 cgattcgcgg ttctggtgct gcgagcgctc gttctggctc tgagcggtga tgccggtggg
     3481 gatgtgcgtc aggcgcacgg ccgagtcggt cttgttgacg tgctggccgc cggcgccctg
     3541 cgagcggaaa gtctcgatgc gcagctcgtc ctccggtatg tcgccgacct ccgtgtcgtc
     3601 ctcgacctcc ggcacgacct cgaccagggc gaacgtcgta tgccgtcgct tctgcgagtc
     3661 gtaggggctg atgcgcacca ggcgatgcac accgcgctcc gcgcgcaggt ggccataggc
     3721 attgcgacca cggatgtcca cggtgacgct cttgaggccc gcagcctcgc cctcgagctg
     3781 gtcgaggatc tcggtgtgga agcggtggcg ctctgcccag cgcaggtaca tgcgcagcag
     3841 catctcggcc cagtcggtcg cctctgtccc gccggccccg gcagtgatcg tcaggatcgc
     3901 gttgtcggcg tcgtagtcac cggagaacag aaggctggtg cgcagccctt cgtattcggc
     3961 cgagactgcc ccgaattccg agtcgagctc ggcggccaga tcgggatcgg cgccggattc
     4021 cgcgagctgg gccagctcga gcaggtcatc ggcgcgtttt tcgagggcgc gccaggtgcc
     4081 cacctcttca cgaagactgt cggcctcgcg tagcaccttc tgggcggcac gttgatccga
     4141 ccagaagccc tcgacgcttg cgcgcgcgtc gagctcatca acgcgcttct cttttgcggc
     4201 taggtcaaag ccgccccgag gtcgcgtgga cgcgctcgac gagctcgcgc aaggcagcga
     4261 tctccatcag ccaggtattg cttcgactag ttcctggcgc gcaggtcctg gagcaagatc
     4321 ggccgcagct cgatgatctt caacgcggtc ttgctcctgg cgacgacggg gttgacacat
     4381 ggacagtatc cgttgtgggg acataccccg cagttgccgt cgcaatcgag cgttgcggcg
     4441 taactgcagg ttcggcactc tcgctcacag gcgatgagat ccacgaatgg atcggctagt
     4501 gcctccttcg ccggcttgag gttcacgcct cgccagactc cttgttgagc cagggcaccc
     4561 tcgaccccgg ggacgtggtt aggacacgtt caccggagga ggcacggcct ccggaggcgg
     4621 gtacgtccga gtatgccagc cgaggcgctc acgctcaata gggaattttg atttgcatcg
     4681 attcggctgc gatccccgac ggcgtgatct gctcggtcag cagccgggag aaccgcgcat
     4741 gttgggcctc cttgtcaccg gccgattcga cccaatggct caatagccgc tcgcctcccg
     4801 catagcagaa gacataggtc cgccacaggg ggtggtcgat gaactcgagg accttgaggg
     4861 cgcgctcgtg ggtcgatagc gcgtcctgct cgaggaagcg cacgacgtcg tcgcgcgacc
     4921 gcttggccac gtgcagctgg agtgcggcgt cgccgcccgc gccgcgcaac ctgtgcaagg
     4981 cgttcgaggt gtgccattcg cgttgcgcgt cgtcggtggt gagcgcgata ccggcgcgct
     5041 cggtcacgcc gagcagcatc tcctggaagc gcgtctcatt gttgagcaac cacgcgccga
     5101 cttcggcgag gccctcgctg atgtatgact cgggcgtgtt gatgagcatc gcgctcgcct
     5161 cgccgtagcc gcgttcgcga tagagccgtg cttccttcca ggcgtgctcc atgtgatggc
     5221 cggggaatgt ctcgtgggcc agggtgaggg cgacctggtg ggcgcgcatg ggcaggtcgg
     5281 tgttgacctc gacgcgcgac ttcagattgc cgtcgtacca gttgtaggcc gcccacggtt
     5341 cgtcgcgcac catgctgaag gaaagctctt caccatcggg cagcggccag atggcggcgg
     5401 ccgcggagcg caactccgcc gcgacccagt cgatgatcgc cggcagccga tccgcgggca
     5461 ccgtcaggcg ctcgtcgcgc tgggccaggc gatcgcgcag gtcgccgtca ccaggcagca
     5521 gctcttcgag ctcggcccgc gttcgcgcgt agtcctccgg tggcgtcggt tctggcgccg
     5581 cgtcgaagca gtgctccacc tccacgagat acggcagcgt ctcgccggcg agccggcgcg
     5641 cgatggtttc caggccgagc agctggcgat cgagccacag cgcccgttgc tgctcgtctg
     5701 tctcgtcatt cagccgcctg cgcaatgccg ccgcgcggcc ggcgagcgcg gcagcgccat
     5761 cgttgccgct cttcgcttgc tcgcgcagct cgggcggccc gtagtacgag tcgacgatgc
     5821 cgtgctcgat ctcgtcgatg gccagcgcga tcagcaggta ttcgcgcgcg atatcgtcca
     5881 tcagggcgcc acttcagtgt tggcgtcgac gagccgcgcg cgcagccgcg ccagttgctc
     5941 ggaggtcatg ccgccgtcga gcaggtagcg ctccgcgccg ccgtagcgct cacgcaaata
     6001 agcgagcgta tccagcatcg cttcgcgact gg
//
LOCUS       DATBRX010000097         4866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1929950, whole genome shotgun sequence.
ACCESSION   DATBRX010000097 DATBRX010000000
VERSION     DATBRX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929950"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /gene="mreB"
                     /locus_tag="VIF08_03490"
     CDS             <1..621
                     /gene="mreB"
                     /locus_tag="VIF08_03490"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEY8135078.1"
                     /translation="IGAGLPVAEPTGSLIVDIGGGTTEVAVISLGGIVVSRSIRIGGD
                     ELDQDIIAYARREYNLLLGERTAEDVKIALGSAYPGDYDEQRVMLRGRDLLTGLPRAV
                     EITAEQLREAIEPSVSQIVDEIKDTIEETPPELVADIMDQGIVLAGGGALLQGLDRRV
                     SEATQMPVHVADDPLTCVVRGTGKALEELDTMERTLVSETYSHAPR"
     gene            671..1564
                     /gene="mreC"
                     /locus_tag="VIF08_03495"
     CDS             671..1564
                     /gene="mreC"
                     /locus_tag="VIF08_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY8135079.1"
                     /translation="MLTNRSLRRQIVVYVVLLAIAMLMLAFSSSPPVTQLRRGVGYAM
                     APIQEVLRGAGQTVGSVFAAFGEIERLRQQNEDLARRLNEVEAENQGLVSLREQNELL
                     TQLLDVRSSFEYETVAAEVISKRITDQERAISLDRGADAGIEVDDPVIGPGGALIGQV
                     VEVGANFSRVLLISDTRIHVAGLIESSRAVGLLSGKLGQPLEMVNIPAEVTVSANESV
                     VTAGIELAEGIRSPYPKGLLIGLVAGVSRSPDQLFQTALVTPVADLDRLEYVLVITSY
                     EGGLPDTAPSPSPTLEPSAAP"
     gene            complement(1580..2113)
                     /locus_tag="VIF08_03500"
     CDS             complement(1580..2113)
                     /locus_tag="VIF08_03500"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8135080.1"
                     /translation="MVRQPMEPEFPDAPEVVGDVREDDDLEPEPKRPRRRGAADTALP
                     WVLATVVVLLLAVSAGLFAAWVVASLKAVPIPAGAIVTASPVPGATDVPSTQPTSPSS
                     GQPRHTPTPAPALTPEPPPFVHVVQRGEYLTLIADMYDVRVEDILALNDIKDPDRIRP
                     GRELLIPGYGIRPSPSP"
     gene            2412..3161
                     /locus_tag="VIF08_03505"
     CDS             2412..3161
                     /locus_tag="VIF08_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEY8135081.1"
                     /translation="MTSEVLTGTWVVLPTYNEIENLPLIAPAILGQLPEATLLVVDDS
                     SPDGTGDLANRMALENPRVKVLHRAAKQGLGKAYTDGFRHALESGAELLIQMDADWSH
                     DPTYLPGMVATLQNSADLVIGSRYVKGGGVRNWGILRRLVSRGGSIFARTVLGLKAHD
                     LTGGFKAWRRPTLAALPWASLHSGGYVFQIETTFRAARAGARVVEVPIVFVDRRLGAS
                     KMSRSIILEALLVVIRLRWDELRGRRGPKGA"
     gene            3227..3697
                     /locus_tag="VIF08_03510"
     CDS             3227..3697
                     /locus_tag="VIF08_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135082.1"
                     /translation="MDADEQHSVPSDDDSDAPVSFEDTTPAHHNGHLPDGMDASGDVR
                     AENVTISQGGARDIVATTVSITQGGAAQVRAEQMTLSQGGVALAKTDNLTVADGGSAF
                     AVLSDHATVNEGGNVLVLLAGEVSGDARPLLDWRSALALGAGLVVAWRLVRRIF"
     gene            3713..>4866
                     /locus_tag="VIF08_03515"
     CDS             3713..>4866
                     /locus_tag="VIF08_03515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8135083.1"
                     /translation="MTYRPRRQSRPGLGTRVRRLFEGEERQQATVTALFIGAIVIVVL
                     ALIGAVALAWYNDNLRPLARVGTFEIPPQLLRDRVELETWRIGRDQDRVAVALQNGEI
                     DEATATSRTQALQEQLNSLTTDALENLIDIVFQSQLAPAEGITVTPEQVDGLLAKEFA
                     GVEKRHMWEIVVKPIAADAAEGPTGSEQRAALAKAEEALAAINAGGDWATIAHQYSNG
                     DSEPAGGDLGIATANKISDLEFQRQLFELPLNGTTGIVRGEDGFYRIGRVTEILPAGE
                     QAGLRDQLFTRLPESNVRALLNYEAAAAALHDKITNDALAQTPEQARIAVIFIEGEVS
                     EDPTAAQGEIDYSEIVFAPNNSLEDTPPADDPAWAQALTDAQAVYDQLKA"
BASE COUNT          803 a         1672 c         1672 g          719 t
ORIGIN      
        1 atcggcgccg ggctgccggt cgcggagccg acaggctcgc tgatagtcga catcggcggt
       61 ggcacgaccg aggtcgccgt catctcactc ggcggcatcg tggtcagccg cagcatccgc
      121 atcggcgggg acgagctcga ccaggacatc attgcctacg ctcgccgcga gtacaacctg
      181 ctgctgggcg agcgcaccgc cgaggacgtc aagatcgcac ttggctccgc gtacccgggc
      241 gactacgacg agcagcgcgt catgctgcgc gggcgcgacc tgctgacggg gctgccgcgc
      301 gcggttgaga tcaccgccga gcagctgcgc gaggccatcg agccgtcggt ctcgcagatc
      361 gtcgacgaaa tcaaggacac catcgaggag actccgcccg agctcgtcgc cgacatcatg
      421 gaccagggca tcgtcctcgc cggcggcggg gcactgctgc agggtctcga tcggcgcgtg
      481 agcgaggcga cccagatgcc ggtccatgtc gccgacgacc cgctgacgtg cgtcgtgcgt
      541 ggcaccggca aggcgctcga ggagctcgac acgatggagc gcacgctagt ctcggagacg
      601 tactcacacg ccccgcgctg agtccgtcgc gacgggctga gcgcccagta aatgggaaca
      661 gggtgcacca gtgctgacta atcgttcgct ccgacggcag atcgtcgtct acgtggtgct
      721 gctcgcgatc gcgatgctga tgctggcgtt cagcagctca ccgcccgtga cccagctgcg
      781 ccgcggcgtc ggctacgcga tggcgcctat ccaggaagtg ctgcgcggcg caggccagac
      841 cgtcggctcc gttttcgcgg cgttcggcga gatcgagcgg ctgcgccagc agaacgagga
      901 tctggccagg cggctcaacg aggtcgaggc ggagaaccag ggcctggtca gcctgcgcga
      961 gcagaacgag ctcctgaccc agctcctcga cgtccgttca tcgttcgagt acgagacggt
     1021 tgccgccgag gtgatcagca agcgcatcac cgaccaggag cgggcgatca gcctcgatcg
     1081 cggcgccgac gccggcatcg aggtcgacga tccggtcatc ggcccgggcg gcgcgctgat
     1141 cggccaggtc gtggaagtcg gcgccaactt cagtcgcgta ttgctgataa gtgacacccg
     1201 gatccatgtc gctggattga tcgagtcgag ccgcgccgtg ggactgctga gcggcaagct
     1261 gggccagccg ctggagatgg tcaacatccc tgccgaggtg acggtcagcg caaacgagtc
     1321 ggtcgtcacc gcgggcatcg agctcgccga aggtatccgc tcgccgtatc ccaagggact
     1381 gctcatcggc ctggtcgccg gcgtcagccg ctcgcccgat cagcttttcc agaccgcgct
     1441 ggtcacaccc gtggcggacc tcgatcgact cgagtacgtg ctcgtgatca ccagctacga
     1501 gggcggtctg cccgacacgg cgccttcacc ctcgcccacg ctggagcctt cggccgctcc
     1561 ctagagaagc ctcgtccgct tacggtgacg gcgagggccg gatgccgtag ccggggatca
     1621 gcagctcgcg cccgggacga atgcggtcgg gatccttgat gtcattcagg gcgaggatgt
     1681 cctcgacgcg cacgtcgtac atgtccgcga tcaacgtcag gtactcgccc ctctgcacga
     1741 cgtgcacgaa tggcggcggc tccggcgtca gagcgggcgc gggtgtcggg gtgtggcgcg
     1801 gctgcccgga cgacggcgac gttggctgag tgctgggcac gtcggttgcc cctggcaccg
     1861 gcgacgccgt gacgatcgcg ccggccggga tgggcacggc cttgagtgag gcgacgaccc
     1921 aggctgcgaa cagccctgcg ctcacggcca gaagcaggac gacgaccgtg gcgagcaccc
     1981 acggcagcgc cgtgtcagcc gccccgcggc gacgcggtcg cttgggctcc ggctcgaggt
     2041 cgtcgtcctc gcggacgtcg cccaccactt cgggcgcgtc ggggaattcg ggctccatgg
     2101 gctgcctgac cacgcgcgca gagtagccaa cgacagccca gtgcgttcca cggcccaact
     2161 tgaccccacc gcacatcctc aaggaccgcc aacggtgccg ggtggcgcgg gaccatcttg
     2221 gtatggcacg gcgcgccggt caccgggagg cttgacgcgg tgggctctcc ggcagccgtg
     2281 atctggttga gccccttcgc aatccagcgg gtcgcagcct ccgacccgcg gacaggtatc
     2341 gccgagccgg cccgcgaggg ccggctcggc gcttcaaccg acgttcctgg gcgcaggtag
     2401 actcgcgggc agtgacttca gaggtgctga ccggcacgtg ggtcgtgctg ccgacgtaca
     2461 acgagatcga gaacctgccg ctcatcgcgc ccgcgatcct gggccagctg cctgaagcga
     2521 cgctgctcgt cgtcgatgac tcgtcgcccg acggcaccgg cgatctggcc aaccgcatgg
     2581 ccctcgagaa cccgcgcgtc aaggttcttc accgcgcggc caagcagggc ctgggcaagg
     2641 cctatacgga cgggttccgg catgcgctcg agagcggtgc cgagctgctc atccagatgg
     2701 acgccgactg gtcgcacgac ccgacgtatc tcccgggaat ggtcgcgact ctgcagaaca
     2761 gcgccgatct cgtcattggg tcgcgctacg tgaagggtgg cggcgtgcgc aactggggca
     2821 tcctgcgccg ccttgtatcg cgcggcggct cgatcttcgc gcgcaccgtc ctggggctga
     2881 aggcgcatga tctgaccggc ggcttcaagg cgtggcgccg cccaaccctc gcggcgctgc
     2941 cgtgggcaag cctccactcg ggcggctacg tcttccagat cgagaccaca ttccgcgcgg
     3001 cgcgcgccgg ggcgcgggtg gtggaagtgc cgatcgtctt tgttgatcgc cgcctggggg
     3061 cgtccaagat gtcacgaagc atcatcctcg aggcgctcct ggtggtcatt cggctgcgct
     3121 gggacgagct ccgcggccgg cgcggcccca agggcgcctg acgcccgcga tcacagacac
     3181 cccacaggca gcgcgatcgg gcgcggcgcg gtacgctggc gcccccatgg acgctgacga
     3241 acagcacagc gtgccgtccg acgacgactc ggacgcgccg gtgagcttcg aagacaccac
     3301 acccgcccat cacaatggcc acctgcccga cggcatggac gcgtccggtg acgtgcgcgc
     3361 ggagaatgtg acgatcagcc agggcggggc gcgcgacatc gtcgcgacga cggtctcgat
     3421 cacccagggc ggcgcagccc aggtgcgcgc cgagcagatg accctcagcc agggcggcgt
     3481 cgccctggcc aagaccgaca acctgaccgt cgccgacggc ggctcggcat tcgccgtgct
     3541 ctctgaccac gccaccgtga acgagggcgg caacgtcctc gtgctgcttg ccggcgaggt
     3601 cagcggcgac gcgcgaccac tgcttgactg gcgctcggcg ctggcccttg gcgccgggct
     3661 cgtggtcgcc tggcgcctgg tgcggcgcat tttctaggag acacgcaatc aaatgactta
     3721 tcgaccacga cgccagtcgc ggcctggcct tggcacgcgc gtacgtcgtc tcttcgaagg
     3781 tgaggagcgt cagcaggcca ccgtcaccgc gctgttcatc ggcgccatcg tcatcgtcgt
     3841 gctggcgctc atcggagcgg tcgcgcttgc ctggtacaac gacaacctgc ggccgctggc
     3901 gcgcgtcggc acgttcgaga tcccaccgca gctgctgcgc gatcgcgtcg agctcgagac
     3961 gtggcgcatc gggcgcgacc aggaccgcgt cgccgtagcc ctgcagaacg gcgagatcga
     4021 cgaggccacc gccacatcac ggacccaggc gctgcaggag cagcttaatt cgctgactac
     4081 ggatgccctg gagaacctga tcgacatcgt cttccagtca cagcttgcgc cagcggaggg
     4141 catcacggtc acgcccgagc aggtcgatgg gctgctcgcc aaggagttcg cgggcgtcga
     4201 gaagcgccac atgtgggaga tcgtggtcaa gcccatcgcg gccgacgcag ccgagggccc
     4261 cacgggttct gagcagcggg ccgcgctcgc caaggcggag gaggcgctcg ccgccatcaa
     4321 cgccggtggc gactgggcga cgatcgccca tcagtacagc aacggtgaca gcgagcccgc
     4381 cggtggcgac ctgggcatcg cgaccgccaa caagatcagc gacctggagt ttcagcgcca
     4441 gctcttcgag cttccgctca acgggaccac cggcatcgtc cgcggcgagg atggcttcta
     4501 tcgcatcggc cgggtgacgg agatcctgcc ggccggtgag caggcaggcc tgcgcgacca
     4561 gctcttcacc cgtctgccgg agtccaacgt gcgcgcgctg ctgaactatg aggccgcggc
     4621 ggcggcgctt cacgacaaga tcaccaacga cgccctggcg cagacgccgg agcaggcgcg
     4681 catcgccgtc atcttcatcg agggcgaggt cagcgaggat ccaaccgccg cgcagggtga
     4741 gatcgactac agcgagatcg tgttcgcgcc aaacaacagc ctcgaagaca ccccgccagc
     4801 cgacgatccg gcatgggcac aggcgctcac ggacgcacag gcggtctacg accagctgaa
     4861 ggcgat
//
LOCUS       DATBRX010000098        11190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1930280, whole genome shotgun sequence.
ACCESSION   DATBRX010000098 DATBRX010000000
VERSION     DATBRX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1930280"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /gene="cysS"
                     /locus_tag="VIF08_03520"
     CDS             complement(<1..1344)
                     /gene="cysS"
                     /locus_tag="VIF08_03520"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY8135084.1"
                     /translation="MTGLHLTNTLGGALEEFTPLDPANVRVYSCGPTVYGPSHVGNFR
                     AFLLGDLLSRYLRWSGYTVTWVMNITDVDDRIIRDLAAHGGTLDELTAPHIAGFLENL
                     GKMRIGPPDKLTRATEHINEMAALIARLQANGHAYKTDDGSIFFDISSWPAYGVLAKL
                     EPGAARQTERVAADDYGKEDVRDFALWKGPRDGEPSWDTEVGPGRPGWHIECSAMSMK
                     HLGESFDIHTGGVDLIFPHHENEIAQSEAATGKKFVTTWLHNAHLQLRGIKMARRIGN
                     IEKPAEVFAEGWTPIELRYALLATHYRAPLDWGDETLEHARAAVERLAAALAALDAYE
                     EERADDPDLPAALKSARDAFKLAMDDDLNVSGGLAAVMELVRDLNRRVDARSLSTADA
                     RRGVDALRDLDQVLGLLPEAQTLPDGAAALLEERAAARAAKDWAKSDELRDRLAGL"
     gene            complement(1352..2533)
                     /gene="ispF"
                     /locus_tag="VIF08_03525"
     CDS             complement(1352..2533)
                     /gene="ispF"
                     /locus_tag="VIF08_03525"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY8135085.1"
                     /translation="MSSNDRADSETARDAKPFADAVIVAAGGSTRMGGSDKMAEPLLG
                     RSLLQWSVENMAAAQTVARVIVVARPDRVADLAAQPWLSDATVVAGGERRSDSVRAGV
                     TAATAEVVLVHDGARPLASPALADAVANATAQHGAAVPVVPVVDSLKRDNGATLGESV
                     KRDGLVRTQTPQGARRQLLLDAMAAAGNVAFSDEAALLESQGIAIATVPGEPRNIKVT
                     LPQDLEMVRALARGGAETRVGLGQDSHAFGPDLGLWLGGIQIEAAPRLYGHSDGDVIL
                     HAIATAILSATGNGDLGRLFPSDDRQTTGIASSDLLREAIAKAGQSGWAVESAQVSVV
                     GARPRLGGQRLDAMAANVAGLLAVDRDSVAITASTGNLSGDEGAGRVISATALIGLRR
                     R"
     gene            complement(2551..3612)
                     /locus_tag="VIF08_03530"
     CDS             complement(2551..3612)
                     /locus_tag="VIF08_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8135086.1"
                     /translation="MNSFIRLFGGALGALIAVVLAGGARIPPLDLSSIGGVVLLVLWV
                     AAWAVIGFSILPYVTVLPARWLIGRVMTLTAGEFVSAAAGLVAGLVIGALIGLPMANL
                     PEPYRWVLPIGTVVVTGLGMMGLTVAKRHDLAEGLRSAGVLKPPATDSPVVPIEGPVI
                     YVDTSVLIDGRLTDVVASGFLWGTLVVPRFVVAELQHIADQRDAGRRARGRRGLEILA
                     VLQKDPRIAVELPDEDVAAETEVDAKLIALARRRGAAILTNDFNLNRVAQLDDVRVLN
                     LNHLANAIKPAFLPGDTLHVKVTAQGKEAGQGIAYLDDGTLIVVEGGTDLIDKEVDVN
                     VTRVLQTVAGRMVFAQPRS"
     gene            complement(3609..5033)
                     /gene="radA"
                     /locus_tag="VIF08_03535"
     CDS             complement(3609..5033)
                     /gene="radA"
                     /locus_tag="VIF08_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY8135087.1"
                     /translation="MAKQGDRAYVCGTCGATSLRWEGQCRSCSGWNTLVETVVERPRG
                     ATGARRKAVAAPPVTPLNEVAAHDTDRIVIGLREVDRVLGGGFVPGSLVLLGGEPGIG
                     KSTLVLAVCGRVAGPARVLYASGEESAAQLRLRATRLGLDGATGIDVLAETSVERIVA
                     AAEASNPALLVVDSIQTLTTDELDGPQGSVGQVRAAAARLQSYAKENAVPVLLVGHVT
                     KDGTLAGPKTLEHLVDVVLTLEGERFSGLRLLRSAKNRFGSTEEVGVFEMAGTGLVEV
                     ADPGGAFLEADSLGAPGVAVAATLEGSRPLLVEVQALVAPQVHGSPRRTSAGLDNQRL
                     ALLIAVLGRRAGMNLASHDVYANIVGGLTVEEPTLDLPLALALASALRDRPLAPGTVS
                     CGEVGLTGELRPISGLGRRLREAARLGFTRAIVPRNVGRGEDLQDVKIDLVRVGTLRE
                     AISAALVERPRDEARAIPVAIDAA"
     gene            complement(5050..7527)
                     /locus_tag="VIF08_03540"
     CDS             complement(5050..7527)
                     /locus_tag="VIF08_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY8135088.1"
                     /translation="MDRFDKFTDRARKVLTLAQDEAQRFNHNYIGTEHLLLGLVREGE
                     GVAARVLENMNVELAKVRTAVEFIIGRGDRPVVGEVGLTPRAKRVIELAIDEARRLGH
                     NYIGTEHLLLGLVREGEGIAAGVLESLGVNLDKVRHEVIRVLSQSSSAGPTTETKRAS
                     KTPTVDQLGINLTDAVRAGKIDPVIGREKEIERVIQILSRRTKNNPALIGEPGVGKTA
                     IAEGLAQRIVAGDVPETLLNKRVLTLDIGSLVAGTKYRGEFEERLKKIIEELRNTNDA
                     VLFIDELHTLVGAGAAEGAIDAANILKPPLARGELQCIGATTLDEYRKYIERDAALER
                     RFQPVMVDEPTREQTVDILLGVRSRYEEHHKVKITDDAVKAAADLSIRYITDRHLPDK
                     AIDLIDEAASRVRLRTASTPPELKEAQKELERVTKEKEAAINDQDYEVAANLRDAEAQ
                     AKEAVDSIRASWQASHSAEMAQVTQEDIAQVVAMWTGIPVTAIAQEESERLMHMEDAL
                     REKVIGQDEAIDTIAKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTWLAKALAEFMFG
                     SEDSLIKIDMSEFMERHNVSRLVGAPPGYVGFDEGGQLTEAVRRKSYSVVLLDEIEKA
                     HPEVFNILLQILEDGHLTDAKGRRVDFRNTVIIMTSNVGAKSLLKDTSLGFKPVAIDQ
                     SKAEEQMYDRMKEKVLDQLKTQFRPEFLNRVDSVVVFRSLQIEQIRMIVELLLARVRD
                     QLRAQQIELEITQEAKDHIIKLGYDIDYGARPLRRVIQNMVEDQLAEALLTGRFSAGM
                     TVRVDKSPDAGLVIEPITEKIPVEAGA"
     gene            complement(7742..9946)
                     /gene="rpsA"
                     /locus_tag="VIF08_03545"
     CDS             complement(7742..9946)
                     /gene="rpsA"
                     /locus_tag="VIF08_03545"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY8135089.1"
                     /translation="MTVEGMEGTDQAEPTADEPTTTDATATENPVAADEMAPDASASA
                     EPAPTEDVPAEEPALSDSAMAELMAMADAQAAAHVEAAQMAEADLAEAPAAAEEQTEA
                     VAESAPEAPVAAAPEADATAAEASPEASAAEAAPEAAPEAALAPAPKPAPRVYEGPEP
                     TTMEELLNEQATEIRSLKHGDVVEGNVVRIDKDEILVDIGAKSEGVISNRELYGRNAE
                     SQPQLNIGDQVLVYVLQPESPEGHAVLSLRRAGLERKWRAMQEQLEAGVIIEAPVIDH
                     NKGGLIVDCGIRGFVPISQIVDFPRRPQNEQPRDAAQEIAEKLQPYVGRKLRLKILEV
                     NRKANRLILSEKVALYEERREKRDELFSSLQVGQKVKGTVRSIAPFGVFVDLGGIDGL
                     VHKSELSWNKVNNPEAGYRVGEEVEAEVIDINHERGRISLSIRRLQPDPWHSTVADLK
                     IGDVIDGTVTKIVNFGAFVRVRDGLEGLIHISELSHQRVAHPGDVVHEGQTLKLKIIS
                     LEQDRHRLGLSLKQAEEAPARPEQPAPSAGPSGGGGAPERRERRPRGGDRGERGGFDM
                     SQAVQEPEGGIDNTLAAAFAQVRQQIEQTEAARESSETEESGEGPAEEAKPKAKAARA
                     KAQPADEPADETAAEPAAEPAAEAKPKATKAKAAKAAPAEEPAPKAKAAAKTAAKPAA
                     KAEAKAAPKAEAKAAPKAEAKAAPKAKAAKPDAAEKPAAKKAAPSKAKPKAK"
     gene            10176..11045
                     /locus_tag="VIF08_03550"
     CDS             10176..11045
                     /locus_tag="VIF08_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY8135090.1"
                     /translation="MPELPDIAILADALDVALAGRPLTGSKIPQTLVLRGTPSELAAF
                     EGQALLDVSRRGKFLVFRFERDRMVFNPMLTGRLGVVTAGEKAWPQWAAMFEFGAATG
                     KARRHTWPPAKATWLPRRDLGVELRYRDATRMGKIYVMPAGVARPVAGWDEQGPDADD
                     PALKLEVWRERIGRHSGELKSLLRNQAFVAGIGNAYSDEILWAAGLLPFRKRPSLAPD
                     EVDALYSATRETVTWAIEQLHELVPPRLEVEQREFLNVHRKGGSACPRCGTRLSQVSA
                     GGFDTTFCRGCQR"
BASE COUNT         1600 a         3843 c         3810 g         1937 t
ORIGIN      
        1 cagccctgcc agccggtcgc gcagctcatc tgacttggcc cagtccttgg ccgcgcgagc
       61 cgcggcacgc tcctcgagca gcgcagccgc gccatcgggc agcgtctgcg cctccgggag
      121 cagaccgagc acctggtcca gatcgcgcaa agcgtcaacg ccccggcgcg cgtcggcagt
      181 cgacagcgac cgcgcatcga cgcgccggtt gaggtcacgc accagctcca tcaccgcggc
      241 caggccgcca ctcacgttga ggtcgtcgtc catggccagc ttgaacgcgt cccgtgccga
      301 tttcaaagca gcggggaggt cgggatcgtc ggccctctct tcttcatacg cgtccagcgc
      361 cgcgagcgcg gcagccagcc gctccaccgc ggcgcgcgca tgctcgagcg tctcatcgcc
      421 ccaatcgagt ggcgcgcggt aatgagtggc gagcaacgcg taacggagct cgatcggggt
      481 ccagccctcc gcgaacacct ccgcgggctt ttcgatgttg ccgatgcgcc gggccatctt
      541 gatgccccgc agctgcaggt gcgcgttgtg cagccaggtc gtgacgaact tcttgcccgt
      601 ggcggcctcg ctctgcgcga tctcgttctc gtgatggggg aaaatcaggt cgacgccacc
      661 ggtgtggatg tcgaacgact cacccaggtg cttcatgctc atcgccgagc actcgatgtg
      721 ccagcccggc cggcccggac cgacctctgt gtcccacgac ggctcgccat cgcgcgggcc
      781 cttccacagg gcaaagtcgc gcacgtcttc cttgccgtag tcgtcggcgg cgacgcgctc
      841 cgtctggcgc gcggcaccag gctccagctt ggccagaacg ccgtacgccg gccacgacga
      901 gatgtcgaag aagatcgagc cgtcatcggt cttgtaggcg tggccgttcg cctgcagccg
      961 cgcgatcaac gcggccatct cgttgatgtg ctcggtcgcg cgcgtgagct tgtccggcgg
     1021 cccgatgcgc atcttgccca ggttctccag gaagcccgcg atgtgcggcg cggtcagctc
     1081 gtcgagcgtg ccgccgtggg cggcgaggtc gcgaatgatc cgatcgtcga catcggtgat
     1141 gttcatgacc cacgtcaccg tgtagccaga ccagcgcagg tagcggctca gcaggtcgcc
     1201 cagcaggaag gcccggaagt tgcccacatg cgacgggccg tagacggtcg gcccgcacga
     1261 gtacacgcgc acattcgccg gatcgagcgg tgtgaattcc tcgagcgcgc cgccgagcgt
     1321 gttggtgaga tggagtcccg tcactccccc atcatcgccg acgcagccca atcagtgctg
     1381 tggcgctgat gacccggccc gcaccttcgt cgccgctgag gttgcccgtg gacgccgtga
     1441 tggccacgga atcccgatct accgcgagca gcccggcaac attggcagcc atcgcgtcca
     1501 gtcgttggcc accgagcctt ggccgggcac cgaccaccga cacctgggcc gactcgaccg
     1561 cccagcccga ctgtcctgct ttcgcgatgg cttcgcgcag cagatcggag ctggcgatgc
     1621 ccgtcgtttg cctgtcgtcg gatgggaaga gccggccaag gtcgccgttg cccgtcgcgg
     1681 acaggattgc cgtcgcgata gcgtggagga tgacgtcgcc gtccgagtgg ccgtacaggc
     1741 gcggcgcggc ctcgatctgg ataccgccca gccacaggcc aaggtctggc ccgaaggcat
     1801 gcgagtcctg gcccaggccc actcgcgtct cggcgccgcc tcgtgccagc gcgcggacca
     1861 tctcgagatc ctgcggcagc gtgaccttga tgttgcgcgg ctcaccggga accgtggcga
     1921 tcgcgatgcc ttgactttcg agcagcgccg cctcgtcgga gaacgccacg ttcccggccg
     1981 cggccatagc gtcgagcagc agctggcgcc gcgcgccctg gggcgtctgg gtgcgcacga
     2041 ggccgtcgcg cttgaccgat tcgccgagcg tcgcgccgtt gtcgcgcttc agtgagtcga
     2101 cgaccggcac gaccggcacc gcggcgccgt gttgcgcggt agcgttggcg accgcatccg
     2161 caagcgcggg cgaggccagc gggcgcgccc cgtcgtggac cagtacgact tcagcggtcg
     2221 cagcggtgac gccggcgcgg actgaatcgg agcggcgctc ccctcctgcc acgaccgtcg
     2281 cgtcgctgag ccatggctgc gcggcaaggt ccgcgactcg atccggccgc gccacgacga
     2341 tgactcgcgc aacggtctgc gcggccgcca tgttctccac cgaccactgc agcagagagc
     2401 ggcccagcag cggctcggcc atcttgtcgg acccgcccat gcgcgtcgat ccaccagcgg
     2461 ccacgatgac cgcgtcggcg aagggtttcg cgtcccttgc cgtctccgag tcagctctat
     2521 cgttggacga catgggcctc aggcatcgcc ttagctgcgc ggctgagcga agaccatccg
     2581 ccccgcgacg gtctggagga cgcgcgtcac gttgacgtcg acctccttgt cgatgagatc
     2641 ggtgccgcct tcgacgacga tgagcgtccc atcatcgagg taggcgatgc cctggccggc
     2701 ctctttgccc tgcgccgtga ccttgacgtg cagcgtgtct cccggcagga acgcgggctt
     2761 gatcgcattg gcgaggtgat tgaggttcag gacgcgcacg tcgtcgagct gggccacgcg
     2821 attgaggttg aaatcattgg tcaggatcgc ggccccgcgc cgacgcgcca gcgcgatcag
     2881 cttggcgtcg acttcagtct ctgcggcgac atcctcgtcg ggcagctcga ccgcgatgcg
     2941 cggatctttc tgcagcacgg cgaggatctc gaggccgcgc cgcccccggg cgcgccgacc
     3001 cgcgtcacgt tgatcggcta tgtgctgcag ctcggcgacg acgaagcgcg gcacgacaag
     3061 ggtgccccac aggaacccgg atgcgacgac gtcggtgagg cggccgtcga tgagcacgct
     3121 ggtatcgacg tagataaccg gaccttcgat cgggacgacc ggagaatcgg tggccggtgg
     3181 cttcaggacg cccgcggagc ggaggccctc agccaaatcg tgccgcttgg cgaccgtgag
     3241 gcccatcatc cccagacccg tgaccacgac cgtgccgatg ggcagcaccc agcgatatgg
     3301 ctcgggtagg ttggccatcg gcagtccgat caacgcaccg atcacgaggc cggccaccag
     3361 ccctgccgct gcgctcacga actcgcccgc ggtgagggtc atcacgcgcc cgatgagcca
     3421 gcgcgcgggc aagaccgtga cgtacgggag gatcgagaag ccgatcaccg cccacgctgc
     3481 gacccacagg acgagcagca ccactccgcc gatggagctg aggtcgagcg gcgggatgcg
     3541 cgcgccgccg gccagcacga ccgcgatgag cgcgccgagc gcgccgccaa agagacggat
     3601 gaagctgttc atgccgcgtc gattgctaca ggaatggcgc gcgcctcgtc tctaggccgt
     3661 tcgactagcg ccgcgctgat cgcctcgcgc agggtgccga cgcgaacaag gtcgatcttg
     3721 acgtcttgca ggtcctcgcc gcggcccacg ttgcgcggca cgatcgccct ggtgaagccg
     3781 agccgcgccg cctcgcgcag tcgccggccg aggccgctga tgggccgcag ctcgccggtc
     3841 agccccacct cgccgcacga cacggtaccc ggcgcgagcg gcctgtcgcg caacgcggag
     3901 gcgagcgcga gggcgagcgg caggtcgagc gtcggctcct cgaccgtcag cccgccgacg
     3961 atgttcgcgt agacgtcatg gcttgcgagg ttcatgccgg cacgccggcc cagaacggcg
     4021 atcaacaacg ccaggcgctg gttgtcgagc cccgcagagg tgcggcgcgg tgagccgtgg
     4081 acctgcggcg cgaccagcgc ctggacctcc acgagcagcg gccggctgcc ctcaagggtc
     4141 gcggctacgg cgacgccggg tgcgcccagc gagtcggcct ccaggaatgc gccgccgggg
     4201 tcggcgacct cgaccagccc cgtgccggcc atctcgaaca cgcccacttc ctcggttgag
     4261 ccaaagcggt tcttggcgga gcggagcagc cgcagcccgg agaagcgctc gccttcaagg
     4321 gtcagcacga catcgaccag gtgctcgagc gtcttggggc cggcgagcgt cccatccttg
     4381 gtgacgtggc ccacgagcag gaccgggacc gcgttctcct tggcgtatga ctgcaagcgc
     4441 gcggcggcgg cacgaacctg gcccacggag ccctgcgggc cgtccagctc gtcggtggtg
     4501 agcgtctgga tagagtcaac gacgagtagc gccgggttgg acgcctccgc ggcggcaacg
     4561 atgcgctcga ctgatgtctc ggcgaggacg tcgatgcctg tagcaccatc aaggcccagt
     4621 cgcgtggcgc gcaggcgcaa ttgcgcggcc gactcctcgc ctgatgcgta caggacgcgc
     4681 gccggcccgg cgacacggcc gcataccgcg agcacgagcg tcgacttgcc gatgcccggc
     4741 tcgccgccga gcaacacgag cgaccccgga acgaacccgc cgccgagcac gcggtcgacc
     4801 tctctcaggc caatgacgat gcggtcggtg tcgtgcgccg cgacctcgtt gagcggcgtg
     4861 accggcgggg cagcaactgc cttgcgccgc gcgcctgtcg cgccgcgcgg ccgctccacg
     4921 accgtttcca cgagcgtgtt ccagccgctg catgagcggc attgaccctc ccagcgcagg
     4981 ctggtcgcgc cacaggtgcc gcagacataa gcgcggtctc cctgtttggc catcctgggc
     5041 gccggcgcct taggcgccgg cctcgacggg gatcttctcg gtgatcggct cgatgaccag
     5101 gcccgcgtcc ggtgacttgt cgacccgaac agtcatgccc gcggaaaagc ggcctgtcaa
     5161 cagtgcctca gccagctggt cctcgaccat gttctgaatc acgcgccgca acggccgcgc
     5221 gccgtagtcg atgtcgtagc ccagcttgat gatgtggtcc ttcgcttcct gggtgatctc
     5281 aagctcgatc tgctgcgccc ggagctggtc gcgcacgcgc gcgagcagca gctcgacgat
     5341 catccggatc tgctcgatct ggaggctgcg gaagacgacg accgagtcga cgcggttcag
     5401 gaactcgggc cggaactgcg tcttgagctg gtcgaggacc ttttccttca tgcggtcata
     5461 catctgctct tcggccttcg actggtcgat ggcgaccggc ttgaagccca ggctcgtgtc
     5521 cttgagcagc gacttggcgc cgacattgct ggtcatgatg atgaccgtgt tgcggaagtc
     5581 gacgcgacgg cccttggcgt ccgtcaggtg gccgtcctcg aggatctgca gcaggatgtt
     5641 gaagacctcc gggtgggcct tttcgatctc gtcgagcagc accacggagt agctcttgcg
     5701 gcgtacggcc tcagtcagct ggccgccctc gtcgaagccg acgtaaccgg gaggtgctcc
     5761 gaccaggcgc gacacgttgt ggcgctccat gaactcgctc atgtcgatct tgatcagcga
     5821 gtcctcggag ccgaacatga actcggcgag ggccttggcc agccacgtct tgccgacgcc
     5881 ggtggggccg aggaagatga aggagccgat tgggcgcttg ggatccttga gccccgcacg
     5941 tgcccggcgc accgccttgg cgatcgtgtc gatggcctcg tcctggccga tgaccttctc
     6001 gcgcagcgcg tcttccatgt gcatgagccg ctcggactct tcctgggcga tcgccgtgac
     6061 cgggatcccg gtccacatcg ccacgacctg ggcgatgtcc tcctgggtaa cctgggccat
     6121 ctcggcgctg tggctcgcct gccagctggc gcggattgag tcgacggctt ccttggcctg
     6181 ggcttccgcg tcgcgcaggt tggcagcgac ctcgtagtcc tggtcgttga tcgccgcttc
     6241 cttctccttg gtgacgcgct cgagctcctt ctgagcttcc ttgagctcgg gcggcgtgga
     6301 ggcggtgcgc aggcggacgc ggctggccgc ttcgtcgatc aggtcgatcg ccttgtcggg
     6361 cagatggcgg tcggtgatgt agcggatcga caggtcggct gccgccttga ccgcgtcatc
     6421 ggtgatcttg actttgtggt gctcctcgta gcggctgcgc acgccgagca ggatgtcgac
     6481 cgtctgctca cgcgtcggct cgtcgaccat gaccggctgg aagcgacgct cgagcgccgc
     6541 gtcgcgctcg atgtacttgc ggtattcgtc gagcgtcgtc gcaccgatgc attgcagctc
     6601 tccgcgcgcc agcggtggct tgaggatgtt cgcggcgtcg atcgcgccct ctgccgcgcc
     6661 cgcgccgacg agggtgtgca gctcgtcaat gaacagcacc gcgtcgttcg tgttgcgcaa
     6721 ctcttcgatg atcttcttga gccgctcctc gaactcgccg cggtacttcg tgccggcgac
     6781 gagtgagccg atgtcaaggg tgaggacgcg cttgttgagc agcgtctcgg gcacgtcgcc
     6841 ggcaacgatg cgctgggcga ggccttcagc gatcgccgtc ttgccgacgc ccggctcgcc
     6901 gatcagcgcc gggttgttct tcgtgcggcg cgacaggatc tggatgacgc gctcgatctc
     6961 cttctcgcgc ccgatgaccg ggtcgatctt gccggcgcga accgcgtcgg tcaggttgat
     7021 gccgagctgg tcgacggttg gcgtcttgga cgcgcgcttg gtttccgtcg tgggtccggc
     7081 gctcgacgac tggctcagga cacgaatgac ctcgtggcgc accttgtcga ggttcacgcc
     7141 caggctctcg agcacgccgg cggcgatgcc ctcgccctcg cgcaccaggc cgagcagcag
     7201 gtgctccgtg ccgatgtagt tgtgtcccag gcggcgggcc tcgtcgatcg cgagctcgat
     7261 gacgcgcttc gcgcgcgggg taagcccgac ttcaccgacg accggccgat cgccgcggcc
     7321 gatgatgaac tccaccgcgg tccggacctt ggccagctcg acgttcatgt tctcgaggac
     7381 gcgcgcggcg acaccctcac cctcgcgcac gaggcccagc agcaggtgct cagtgccgat
     7441 gtagttgtgg ttgaagcgct gtgcctcgtc ctgggcaagc gtcagcacct tgcgcgcgcg
     7501 gtccgtgaac ttgtcgaagc gatccatgac cccaccgatg ctaccaaaga ccgcaccctc
     7561 gccgacccta cttcggtcat acgcatgtcc gaactgatgg gcggttaggt gtggtcagat
     7621 tctcgcggtt cgaggccagc ggcacgagcc ccaatgacgc gctgaatagt taaagaaatg
     7681 ccaatcgccg catccgcggt ggacagcttt cgagataagc gccgcccgtt gcggcgcgtg
     7741 gttacttggc cttgggcttg gccttgctgg gcgcggcctt cttggcggcc ggcttctcgg
     7801 cggcatccgg cttggcggcc ttcgccttcg gcgcggcctt ggcctctgcc ttgggagcag
     7861 ccttggcctc tgccttggga gcggccttcg cctctgcctt tgcagcgggc ttcgccgccg
     7921 tcttggcagc ggccttggcc ttgggcgcgg gctcctcggc gggcgcggcc tttgccgcct
     7981 tcgccttggt ggccttgggc ttcgcctcgg ctgccggctc cgcagctggc tctgcggccg
     8041 tctcatcggc cggctcatcg gcgggctggg ccttcgccct ggccgccttg gccttgggct
     8101 tcgcttcctc cgcgggtccc tcgcctgact cctcggtctc ggacgactcg cgcgccgcct
     8161 cggtctgctc gatctgctgg cgaacctggg cgaacgctgc agcaagcgtg ttatcgatgc
     8221 cgccttcggg ctcctggacg gcctggctca tgtcgaagcc accgcgctcg ccgcgatcgc
     8281 caccgcgcgg gcggcgctca cgacgctcgg gcgcaccacc accaccactg ggaccggcgc
     8341 tcggtgccgg ctgctcgggg cgcgccggcg cctcctcagc ctgcttgagc gacaggccca
     8401 ggcggtgccg gtcctgctcg agcgagatga tcttgagctt cagcgtctgg ccctcgtgga
     8461 cgacgtcacc cggatgggca acgcgctgat ggctcagctc gctgatgtgg atgaggccct
     8521 cgaggccatc gcgcacgcgc acgaaagcgc cgaagttgac gatcttggtg accgtgccgt
     8581 cgatgacatc gccgatcttg agatcagcga cggtggagtg ccacgggtca ggctggaggc
     8641 gacggatcga aagcgagatg cggccgcgct cgtggttgat gtcgatgacc tcggcctcga
     8701 cctcctcgcc gacgcgatag ccggcttcgg ggttgttgac cttgttccac gagagctcgc
     8761 tcttgtgcac caggccgtcg atgccgccca ggtcgacgaa gacgccgaac ggggcgatcg
     8821 aacggaccgt gcccttgacc ttctggccca cctgcaggct gctgaagagc tcgtcgcgct
     8881 tctcgcgccg ctcctcgtac agcgcgacct tctcggacag gatcaggcgg ttggccttgc
     8941 ggttgacctc gaggatcttg aggcgcagct tgcggcccac gtagggctgc agcttctcgg
     9001 cgatctcctg ggccgcgtcg cgcggctgct cgttctgggg ccggcgcggg aagtcgacga
     9061 tctggctgat cggcacgaag ccgcggatgc cgcagtcgac gatgaggccg cccttgttgt
     9121 ggtcgatgac cggcgcctcg atgatcacgc ccgcctcgag ctgctcctgc atggcgcgcc
     9181 acttgcgctc taggccggcg cggcggaggc tgagaacggc gtggccctct gggctctccg
     9241 gctggaggac gtagaccagc acctggtcac cgatgttcag ctgcggctgc gactcggcgt
     9301 tccggccata cagctcgcgg ttgctgatga cgccttcgct cttggcgccg atgtcgacga
     9361 ggatctcgtc cttgtcgatg cgtacgacgt tgccctcgac cacgtcaccg tgcttcaggc
     9421 tgcggatctc ggtcgcctgc tcgttgagca gctcctccat cgtcgtcggc tcggggcctt
     9481 cgtagacacg cggcgcgggc ttgggcgccg gtgcgagggc ggcctcaggc gccgcttctg
     9541 gggccgcttc tgcggcagaa gcttctgggg acgcttctgc ggcagtggcg tcggcctcgg
     9601 gcgctgcggc gacaggcgct tctggggcgg attcggcgac cgcttccgtc tgctcctcgg
     9661 ccgcggcggg cgcctcggcc aggtcggcct ctgccatctg cgcggcttcg acgtgcgcgg
     9721 ctgcctgggc gtcggccatc gccatcagct ccgccatggc ggaatcgctc agggccggtt
     9781 cttcggccgg gacgtcctcg gtgggagcgg gctccgcgga cgcggatgcg tcgggcgcca
     9841 tctcatcagc ggcgacgggg ttttcggtcg ccgtggcgtc cgtggtggtg ggctcgtcgg
     9901 cggtcggttc cgcctgatcg gtcccctcca tgccttctac ggtcaaagtt gtgttgtcct
     9961 ccgatcacaa gctcctcgcg gaaaacaaca agacccgagc atctgctcgg gcctccgaca
    10021 tcgggattgt ctgcaggtgt cctggtgggc tcttcagact cctttgacgt gaacctgagc
    10081 gacgctcttc ttgctgctcc ccgtagggcc tgcgcatcct accacgcgcg tgcgcaattt
    10141 cacgcgcaaa cgaaggtgcg ccgtagagtg gcgccgtgcc tgagctgccg gatatcgcga
    10201 tcctcgccga cgcgctcgac gttgcgctcg ccggccggcc gctgacgggc tcgaaaatcc
    10261 cgcagacgct ggtgctgcgc ggcactccgt ctgagctcgc cgctttcgaa ggacaagcgc
    10321 tgctggacgt ctcgcgccgc ggcaagttcc tggtctttcg cttcgagcgc gaccggatgg
    10381 tcttcaaccc catgctgacc ggccggctgg gcgtcgtgac cgccggcgag aaggcgtggc
    10441 cgcaatgggc agcgatgttc gagttcggcg cggccacggg caaggcgcga cgacacacgt
    10501 ggccccccgc caaggcgacg tggctgccgc ggcgcgacct gggggtggag ctgcgctacc
    10561 gcgatgcgac gcgcatgggc aagatctacg tgatgccagc gggcgtggcg cgaccggtcg
    10621 cgggctggga cgagcagggc ccggacgcgg acgacccggc gctcaagctc gaggtctggc
    10681 gggagcggat cggccgccac tccggggagc tcaagagcct gctgcgcaac caggcgttcg
    10741 tggcgggcat cggcaacgcc tattcggacg agatcctgtg ggccgccggg ctgttgccgt
    10801 tccgcaagcg cccgtcgctc gcgcccgacg aagttgatgc cctgtacagc gcgacgcgcg
    10861 agacggtcac ctgggcgatc gagcagctgc acgagctggt cccgccacgg ctcgaggtgg
    10921 agcagcgcga attcctcaac gtgcatcgca aagggggcag cgcctgccca cgctgtggga
    10981 cgcgcctctc acaggtgtcc gcgggtggtt tcgacacgac cttctgccgt ggctgccagc
    11041 gctaagcggc ctcggcctcg ctgaggaacc gctggaatca aaaagaaccc ggccgcgggc
    11101 cgggttcacg atgaagccaa gaatcaagaa ggatcctggc gacgacctat tttcccgagg
    11161 agctaccccc tgagtatctt cggcgctgga
//
LOCUS       DATBRX010000099        17547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2712827, whole genome shotgun sequence.
ACCESSION   DATBRX010000099 DATBRX010000000
VERSION     DATBRX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 17547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17547
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2712827"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..839)
                     /locus_tag="VIF08_03555"
     CDS             complement(159..839)
                     /locus_tag="VIF08_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135091.1"
                     /translation="MGRRCAWPIVVLLVGASVAGCASAAAPTATPGATRPQALAAGTY
                     RSQAFQPRVEFTLPAGWWIASDVADYLALQPVDNDQIGIHLFRDPMPASQDLDCPLEP
                     EPDVGTLSSELSAWMRTLPGLSAGSPRLASVGGLRGTELDLRIADGWQASCPFAGGMP
                     TVPLFVGQDGGLRWVIAGSEQLRLSLLDVPGGGTVVVDIDAFDGLLMNSLLSAANPIV
                     GSMAFPSP"
     gene            1000..1683
                     /locus_tag="VIF08_03560"
     CDS             1000..1683
                     /locus_tag="VIF08_03560"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8135092.1"
                     /translation="MPRTVNQRKHTARRDEFIDAGQRLILTRGFDQFSVEDLLAEVGA
                     SKGAFYHYFDSKQALLEAMIDRMVVSAMGVVGQVVEDTGLTAPEKLRAYFRTIARIKA
                     GQREFLLQLMKVWYSDDNAIVRQKLRRGQIERVTPHLAKIVRQGIAEGAFALADPDQM
                     ARVVLALILDTGDETGELFMKHQAGEIDFATVWRLFETYELALERLLGVPAGTLDLMD
                     EVTLRTWFE"
     gene            1719..2627
                     /locus_tag="VIF08_03565"
     CDS             1719..2627
                     /locus_tag="VIF08_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135093.1"
                     /translation="MSAVIHTEKLTKFYGKHRGMIDIDLDVEEGEAFGFLGPNGAGKT
                     TMIRTLLDHIRPTSGRATVFGIETTVDPVAIHRRLGYLPGEFALYDKLTGGQTLDYFA
                     NLRGGIDKLYQQDLIARLDVDPSRKFREYSKGNKQKIGLIIALQHKPDLLLLDEPTSG
                     LDPLIQQEFYGVIREAKHEGRTVFLSSHILSEVEKTCDRVAIIRDGRLTRVDRTEALR
                     DLAHHTVELVFTGPVPAAEFEALPGVSGVVATDHVLRMKVSGSITPVVRAAAKYELAD
                     FVSREPSLEETFLAEYSKAPAEAAVQ"
     gene            2624..4303
                     /locus_tag="VIF08_03570"
     CDS             2624..4303
                     /locus_tag="VIF08_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135094.1"
                     /translation="MTAATQSIAQPPTLWRRLYGFGSIFGKAMRDSRRAMIVTGALLA
                     IIFLGVSKALVAEFDTPESRIELENLTRAIPPILAGLGGKITDNIATMGGYLQYKYGT
                     FIPLIVSLWSILALSGTLASEAQRGSLDFLATTGKSRARIALEKLAGHIVPLSIALVV
                     LFVSIVIAGSAYPVLPGDEISVTSAFAYTVWVGLMALAAGSLAFALGPFVGRGAAAGI
                     AGFVTFAGFILNGYQDPVPALAPFASLTWWGWTSNHLALAGYYDWPSVGLVALLVVVL
                     LYVGVTAFTRRDIGVTSALPTPSMPRALRGLGGPVGRASALNLGAAIAWGAGLGLFGL
                     ILGNTSRDFLEQIQKSPMFLQLLQQAFPNTDYASAGGFLQLLFVQFGVVLAGLAAATF
                     VAGWASDETSGRLEMVLATPLSRLRWAFASGTGALFNVGVFVALVAGGIALGISSSGG
                     DVATPVIGSLILGVYAAALVGIGLAVGGVLRTSWAAPAVVIVVLLTWLVQLLGPLLGL
                     PDFVQNLALTAHLGQTMVGVWDWGGVAASLALALGGIALGAWGLQRRDLVR"
     gene            4317..5252
                     /locus_tag="VIF08_03575"
     CDS             4317..5252
                     /locus_tag="VIF08_03575"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8135095.1"
                     /translation="MPDLPSGTVTFVFSDLEGSTRLLKQLGDAGFAELLATHRRLVRE
                     TFGRHGGNEIDTQGDAFFYSFARARAAVAAAVEVQRAHVEQEWPRGVKVNVRLGLHTG
                     EPVVGDEGYTGLDVVRAARIAADGKGGQILLSEATRAIVGGNLPDGVAIRELGERTLK
                     DIDQPEPLFELVYAPEPAAAAESALAVAPSEISFDVKPASVLKQWQKLARVAIDAARY
                     DEDPGSAIEERVLAQLKETFGDEIITTGSNPKVKPPKAPKLHDVPGRKGVPAKSVKSG
                     ESVAAGLTRFNELREAGALTDEQYSKAVDRLLSEG"
     gene            5271..6533
                     /locus_tag="VIF08_03580"
     CDS             5271..6533
                     /locus_tag="VIF08_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY8135096.1"
                     /translation="MTDQPLPDRPVTGEATDPHASADPTWRPETLAVHAGMAPDELTG
                     AVAPPIYQTATFAQEGVGRPRGGWEYARTGNPTRARLEQAIAALEGGTHGLAFASGSA
                     TTQVIATLALPGERILCVNDVYGGTFRLFERVLRDTGVDATYHDFAGDSGGELERNLD
                     VRTRLVWLETPTNPHLRVIDIAAIAARLATHVGARGERPILVVDNTFASPLSQQPLKL
                     GADVVFHSATKYLAGHSDTISGVLATSRDDLFARLSFLQNAIGAVPGPMDCFLVLRGL
                     RTLALRIERHASNAQAVAEALAKRDDIEILNYPGLRSGRFAHPNADLAKRQMRYAGGM
                     VSFVPAARDGRSADERARNFVERTRIFTLAESLGGVESLCEIPAVMTHGSVANSPLEV
                     STALVRLSVGVEHVDDLIADVTQALHQA"
     gene            complement(6563..7225)
                     /locus_tag="VIF08_03585"
     CDS             complement(6563..7225)
                     /locus_tag="VIF08_03585"
                     /inference="COORDINATES: protein motif:HMM:NF042831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEY8135097.1"
                     /translation="MKQFLRDNGLSIAMFTIFALCAVGLAVVGWDTFNAEQEEHGRLA
                     IGLADYLATPDFGEAMFENWESEFLQMGAYVLLTVFLFQRGSSESKDPDESKGAADDA
                     TRKGTKRDAPWPVRRGGLVLAIYRHSLSIALFAFFAFSFVMHLITGAARFSEEQLAHG
                     SGPVTAAQYLFTSQFWFESLQNWQSEFLAVGVLIVLSIFLRQKGSPESKSVAAPHAQT
                     GT"
     gene            7322..8692
                     /locus_tag="VIF08_03590"
     CDS             7322..8692
                     /locus_tag="VIF08_03590"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HEY8135098.1"
                     /translation="MDPWQTIIEPFRIHSVEPIRMTTVAEREAALAKSGWNLFNLHAD
                     DVIIDLLTDSGTGAMSRDQWAAIQHGDESYAGSPSWFRFLAAVQKLWPFKHVIPTHQG
                     RAAEKILFSVIGGPGKLVPNNAHFDTTRANVENTGAEAIDLTPPRNPHEMLPFKGDMD
                     VAALERLIKERGVENIPVVFMTITNNSMGGQPVSLANLRAVREVCTRYGLPLFFDACR
                     FAENAWFIKQREPGYAAKSITDIVHEMAALADGMTMSAKKDGLANIGGWLAMNDDALA
                     ERCRNVLILTEGFPTYGGLAGRDLEAIAQGLEEVVEEDYLRYRIQSTAYLGEALDASG
                     VPVIMPIGGHAVYIDAREMLPHIPPLQYPGQSLAVALYRAGGIRGCEIGTVMFGMHPD
                     GTETAAAQDLVRLAIPRRTYTQSHIDYTIEVIQRVAEQASQLRGYKIASAPAALRHFT
                     AVFAPL"
     gene            8793..9227
                     /locus_tag="VIF08_03595"
     CDS             8793..9227
                     /locus_tag="VIF08_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135099.1"
                     /translation="MRKRLALLALPVMVLCLLLPGQAIATPSFSTTVERDTCVAGAGK
                     WGFGQGVLRIRIIEYGKSGANRFTFTAQVWHLPLHGSTWTKEHEWALGVYTFRDNNQS
                     YYNSRQYAWAPNHNAYHKIVIRVRALRDNTVLYSKLFQGKTC"
     gene            9221..9673
                     /locus_tag="VIF08_03600"
     CDS             9221..9673
                     /locus_tag="VIF08_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135100.1"
                     /translation="MLTRLARNVRRAGLLSLALLAFIAPAPTQADPGDFVFEVLRNRC
                     VNTGKDFGHGEVLLKVKVKENGASGANKFTLAAVAQHLKPSTGTWVNEYTFDFVKVTF
                     PDDANSYYHERWFAYDPKESKEHRIVLVMKTLHNKQVLASRTVTSKSC"
     gene            complement(9679..11463)
                     /locus_tag="VIF08_03605"
     CDS             complement(9679..11463)
                     /locus_tag="VIF08_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8135101.1"
                     /translation="MPETTPATIEAPWRRRFRAPRTTLPEWATEAPERLVYAANHVGR
                     FELFAWDRAAGTTHQLTDRREGTIDGHIGPRGENVWWFDDTDGDEFGRWIVQPFDATS
                     GTVAGEELGRFYSTGLQLGREVAVVGGSTESGSTIHVLGGEARQIYANEQESWLGGLS
                     ANEQLLCFHHAEHGDTRHAAIRVVDMDGTVIGDLWDGPGLGLESSGFSQAADDERVLV
                     SHERADLHRPMLWWPRDGRTRTFEIDLPGEIDASWYPDGNALLLVHEHAGRSSLYRLE
                     LEPERLVELPIAPGTVSSAAARPDGEVWYLWSDAATPPEIRSVRADGSAGGVVLRAPG
                     EESPGGVRYTDLLAGDVPAFIAEPTTPRPHPTIFTIHGGPEAHDRDSFQPVVQAWVDH
                     GLAVVQVNYRGSTGHGRAWRDALTGNPGLTELKDIAAVFDAVVAQGIADPARMVLSGN
                     SWGGYLTLLGLSLQAERWTLGVAGVPVADYVAAYEDEMAPLKAYDDALFGGSPTDNLQ
                     AYVDRSPLTFIEQVRVPVFILAGANDPRCPIRQIDNYLDRLVELGKPHEVLRFDAGHG
                     SLRTDERIRQVAAEIDFVARHLGTQPAQ"
     gene            11487..13754
                     /locus_tag="VIF08_03610"
     CDS             11487..13754
                     /locus_tag="VIF08_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8135102.1"
                     /translation="MAEEVGAAGGQTRGFLFADLRGYTDFVERRGDDAAAGLLERYRT
                     LVRGVVARHTGAEVKTEGDSFYVLFPSASSAVAAGLDIVASAAAESAEHPDAPIRVGV
                     GVHAGETSVLPEGPVGSAVNMAARICAQAGAGEVLVSDTVRGLTRTRNVAVFANVGSR
                     RLKGIAEPVALYRAAPPGTIIKGGSRRPTSKALPASWVGIGLAILVLVPATWIIATVL
                     GSAGASPTPAASNASPTAPSAATPTNATSPTAVPVTGTRIIYSRQIGADGCGGVPLDA
                     KLHVLDPDHPAVATYRVSDRSNLLEIEAAWSADGSTIGLVGSVGRGPSGLFTIDASGA
                     PLNVLVAPLSPQALNPFSPPNVTVAPDGSAVVHMDEDALWRTPTDGSGRTQVAGVIRP
                     EDPELEPPPAVERYRDVVYRSDGTLLVLIIGVDGGTPWLETMGDDGSNRTRVELDLEG
                     LDVGGISLAADDDTLALLVPSELGSSIQVGRISSGISDFEVVDFDAENPSQPALSPTG
                     SELVVSGDSNGTEQLYVADLASGEVTQLTDDPDASACSPAWREAPPDLLAGRPALEPG
                     AGGPFELGQLPSGPVLNEVVDPPIQLTFPDGWFARRNYVDGFSVFRPGEVYGEIDYGR
                     LQFNSSGSCDDTELVAVGPLPNDLLSNLQARSDLELAAVGPINLGGYSGITADISGKP
                     EQGCGEPEFRYWSLFRTAQDGFSLSEDEHLRLVALDVRGSTRSFLIFALAPDIDSYWT
                     EKARPILETVVFPEG"
     gene            complement(13757..15364)
                     /gene="pckA"
                     /locus_tag="VIF08_03615"
     CDS             complement(13757..15364)
                     /gene="pckA"
                     /locus_tag="VIF08_03615"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR00224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEY8135103.1"
                     /translation="MATTLDKPTKRALRTSDMPLLGKIRHSNLSVAQLYEHAVRRDEG
                     ILAANGPLVVRTGKHTGRSPKDKYLVDEPDAHANVWWGGFNTPISEERYADLKARVMS
                     HVNEREVFVQEGFVGADPRYRRSLRVYTESAWASIFADNLFIRPKAEDLVGFEPNFTI
                     IDAPSFHADPARDGVRSETVILVHLAKQEILICGTEYAGEMKKGAFGIMNYRLPTEGV
                     LPMHSSVNVGKDGDVAIFFGLSGTGKTTLSADPERVLIGDDEHGWSDDGVFNFEGGCY
                     AKTIRLSHIYEPDIYTTTQRFGTILENVDIDPLTRELNLDSEKFTENTRAAFPLHFIS
                     NSSDVGMAGHPSTIIFLTADAFGVLPPISRLTRDQAMYHFISGYTAKLAGTEVGIKEP
                     TATFSTCFGAPFMPRHPSVYAEMLGERMDRFNVPVWLVNTGWTGGPYGTGERMNITWT
                     RQMVRAALRGALKGVPTRTDPTFGFEVPVTCPDVPAEVLWARDTWADKDAYDAAARKL
                     AGMFVENFKQFEGGVSDAIRDAGPKPA"
     gene            15612..16595
                     /locus_tag="VIF08_03620"
     CDS             15612..16595
                     /locus_tag="VIF08_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8135104.1"
                     /translation="MTLNSSNSSRLDWLLFLVLGFMWGSSYLFIKIGVEAGLPPLTLV
                     MARLIIGGALLLAVVRLAREPFPKTFREYAKLSVLGFFGIALPFYLITVAEASTDSAL
                     AAVLVSPVPLFVIPMSALFLHDEKLTPARILGVVVGLVGVAILVGFDPASLAKNDFGA
                     ELLLVASAISYAVAGVYAKKYIVGYRPMIPALFEVATALVMIGVAAFIFERPWEAPIN
                     VDSVVSVAWLGLFGSGFAFLIFFRLISNWGATRTSTVAYLMPVVGIALGALFLSEEIT
                     PNRLLGTALVIGGVALVNVKREAIASAWAGWRGRTADESAEVAAEVVSDQR"
     gene            16618..>17547
                     /locus_tag="VIF08_03625"
     CDS             16618..>17547
                     /locus_tag="VIF08_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY8135105.1"
                     /translation="MSTTTGSPAHGAATQPISLRTRFSAWAEIMQTANAPIDKPLDTI
                     GKWLVMTRAAVFPMTLWSGTIGALLAVEQARVTGAVSIDWLAVVLAVVGIVLAHAANN
                     LINDFFDQSQGIDSEDYVRALYAPHPVLSGWITRAGLRNAILALTAIDGLILIALALR
                     NPQPALVVAFALAGLFLSVFYVAPPFSLKRRGLGELDVFLTWGPLMIGGTYLVATGTI
                     PAWVIVFSMPYALLVTSVLFGKHIDKIVPDTALGIRTLPVILGEPLARRTGQLLMIAF
                     YPLLAGAVLAGWVGPWVLLAGLAVPELIRALRFF"
BASE COUNT         2796 a         5962 c         5852 g         2937 t
ORIGIN      
        1 gcgccctcgg cgacgcgttg ggcgactgac gcgccgacgt tgccggcgcc gacgacggtg
       61 accttgggac gcgccattac aggcatctcc tcgtgctggg ccacgctgct ggggccgtgg
      121 tctggattgc cccgattgta cgagcgggca gccggcgcct acggactggg aaaggccatg
      181 ctgccgacga tcgggtttgc ggcactcaac aggctgttca tgagcaagcc atcgaatgcg
      241 tcgatgtcga cgacgaccgt gccgccaccg ggcacgtcga gcagcgacag gcgaagctgc
      301 tcgctgccgg caatgaccca gcgcaggccg ccgtcctggc ccacgaagag cggaacggtg
      361 ggcattcccc cggcaaacgg gcacgatgcc tgccagccgt cggcgatgcg caggtcgagc
      421 tcggtgccgc gcaagccgcc tacggacgcg agccgggggc tgcccgcgct gaggccgggc
      481 agggtgcgca tccaggccga tagctcggac gacagcgttc ccacgtccgg ctctggctcg
      541 agcgggcagt cgagatcctg cgatgcgggc attggatccc gaaacaggtg gatgccgatc
      601 tggtcgttat caaccggctg gagcgccagg tagtcggcga catcggacgc gatccaccag
      661 ccggcgggca gcgtgaactc gacccgcggc tgaaacgcct gcgatcggta cgtgccggcc
      721 gccagcgcct gcggacgtgt tgcgcccggc gtggccgttg gcgcggcggc ggaggcgcag
      781 ccagcgaccg acgcccccac caacagaacc acgatcggcc atgcgcagcg tcggcccatg
      841 cggaccatga tgcgttgcgc tgccgctggc ccatcgggcc aacgcacccg cttctggtgg
      901 ggccatatgg cctgaaccaa cgggcggacg gccccgaggc gcgcccaaac cgcagctctt
      961 gacgcgctcg ctattatcag accgacggtc ggtttgtaaa tgccaagaac agtcaaccag
     1021 cgcaagcaca cggcccggcg cgacgagttc atcgacgccg gtcagcgcct catcctgacg
     1081 cgcggcttcg accagttcag cgtcgaggac ctgctcgccg aggtcggggc ttcgaagggt
     1141 gcgttttacc actacttcga ctcgaagcag gcgttgcttg aggccatgat tgaccggatg
     1201 gtcgtgtcgg ccatgggggt ggtcggccag gtcgtcgagg acaccgggct cacagcgcct
     1261 gagaagctgc gcgcctactt ccggacgatc gcgcgcatca aggccggcca gcgcgagttc
     1321 ctgctgcagc tgatgaaggt ctggtactcg gacgataacg cgatcgtgcg ccagaagctg
     1381 cgccgcggcc agatcgagcg ggtgacaccg cacctcgcga agatcgttcg ccagggcatc
     1441 gcggaagggg ccttcgcgct tgccgatccc gatcagatgg cgcgcgtcgt gctcgccctg
     1501 atcctcgaca ccggcgacga gaccggggag ctgttcatga agcaccaggc cggggagatc
     1561 gatttcgcca cggtctggcg cttgttcgag acatacgagc tggcccttga gcgactgctc
     1621 ggggttccgg cggggacgct cgacctgatg gatgaggtga cgctgcgaac ctggttcgag
     1681 tgatttccca tgtcgatttt tgaaaggagt ctcctgcgtt gagcgcagtc attcacaccg
     1741 agaagctgac caagttctac ggcaagcaca ggggcatgat cgacatcgac ctcgatgtcg
     1801 aggaaggcga ggcattcggc ttcctgggtc ccaacggcgc cggcaagacg accatgatcc
     1861 ggacactgct cgaccacatc cgcccgacca gtgggcgagc gacggtcttc ggcatcgaga
     1921 cgacggttga cccggtcgcg atccatcgtc ggctaggcta cctgccgggc gagttcgcgc
     1981 tgtacgacaa gctgaccggc ggccagacgc tcgactactt cgccaacctg cgcggcggca
     2041 tcgacaagct ctaccagcag gacctgatcg cacggctgga cgtcgatcca tcgcgcaagt
     2101 tccgcgagta ctcgaagggc aacaagcaga agatcgggct gatcatcgcg ctccagcaca
     2161 agcccgacct gctgctgctg gacgagccga cctccggcct ggacccgctt atccagcagg
     2221 agttctacgg cgtcatccgc gaggccaagc acgaggggcg cacggtgttc ctctcgtcgc
     2281 acatcctgtc cgaggtcgag aagacgtgcg accgcgtggc catcattcgt gacggtcgcc
     2341 tcacgcgcgt cgatcgcacg gaggcgctgc gcgacctggc tcaccacacg gtcgagctgg
     2401 tgttcacagg tcccgtgccg gcggccgagt tcgaggcgct ccccggcgtg tccggcgtcg
     2461 tggccacgga ccacgtcttg cgcatgaagg tgtcgggcag catcacgccc gtcgtccgcg
     2521 ccgccgcgaa atacgagctg gccgacttcg tcagccgcga gccgtcgctc gaggaaacct
     2581 tcctcgccga atacagcaaa gctccggcgg aggcagcggt ccaatgaccg ccgcgacaca
     2641 gtccatcgcg cagccgccca ccctgtggcg gcggctctat ggcttcggca gcatctttgg
     2701 caaggcgatg cgcgattcgc gccgcgccat gatcgtcacc ggggccctgc tggcgatcat
     2761 cttcctgggt gtcagcaagg cgctcgtggc cgagttcgac acgcctgaga gccggatcga
     2821 gctggagaac ctcacgcgcg ccatcccgcc catcctcgcc gggctgggcg gcaagatcac
     2881 cgacaacatc gccacgatgg gcggctacct ccaatacaag tacgggacct tcatcccgct
     2941 gatcgtgagc ctgtggtcga tcctcgcgct gtcgggcacg cttgcttctg aagcgcagcg
     3001 tggcagcctc gacttcctgg ccacgaccgg caagtcgcgc gcgcggatcg cgctcgagaa
     3061 gctggccggc cacatcgtgc cgctctcgat cgcgctcgtc gtcctcttcg tctcgatcgt
     3121 catcgccggc agtgcctatc cggtcctgcc cggtgacgag atcagcgtca cctcggcatt
     3181 tgcatacacg gtctgggtgg ggctgatggc gctcgcggcc ggcagcctgg cattcgccct
     3241 cggcccgttc gtgggtcgcg gagctgccgc cggcatcgcg ggcttcgtga cgttcgctgg
     3301 gttcatcctc aacggctacc aggacccggt gccggcgctc gcgccgttcg ccagcctgac
     3361 gtggtggggt tggacgagca accaccttgc gctggcgggc tactacgact ggccgtccgt
     3421 cggcctcgtg gcactactcg tggtcgtgct gctgtacgtg ggtgtcacgg ccttcactcg
     3481 gcgcgacatc ggcgtgactt cggccctgcc cacgccgtcg atgccgcgcg ccctgcgcgg
     3541 cctgggtggt cccgtgggcc gcgcgtccgc gctcaacctt ggcgcggcga ttgcctgggg
     3601 cgcggggctg ggcctgttcg gcctgatcct gggcaacacg tcgcgcgact tcctcgagca
     3661 gatccagaag tcaccgatgt tcctgcagct gctccaacag gccttcccga acaccgacta
     3721 cgcgtcggcg ggcggcttcc tgcagctgct gttcgtccag ttcggcgttg tgctggccgg
     3781 cctggccgcg gcgacattcg tcgcaggctg ggcgtcggat gagacatccg ggaggctcga
     3841 gatggttctg gccacaccgc tgtcgcggct gcgctgggca ttcgcgagcg gcacgggcgc
     3901 gctgttcaac gtcggcgtgt tcgtcgcact ggtcgcaggc ggcatcgcgc tgggcatcag
     3961 cagctccggt ggcgacgtgg cgacgccggt gatcggctcg ctcatcctgg gcgtgtacgc
     4021 ggccgcgctg gtcggcatcg ggctggccgt gggcggcgtc ctgcgcacga gctgggcggc
     4081 gccggcggtt gtgatcgtcg tcctgctgac ctggctggtg cagctcctgg gaccactgct
     4141 gggtctgccc gacttcgtgc agaacctggc gctgacggcg cacctgggcc agacgatggt
     4201 gggcgtctgg gactggggcg gcgtggcggc ttcgctggcg ctcgcgttgg gcggcatcgc
     4261 gctcggcgcg tggggcctgc agcggcgcga cctcgtgagg tagaacatac gccctgatgc
     4321 ccgacctgcc gagcggcacc gtcaccttcg tcttcagcga cctcgagggc agcacgcggc
     4381 tgctcaaaca gctcggcgac gcagggttcg cggagctgct ggcgacgcat cgcaggctgg
     4441 tgcgcgagac cttcggccgc cacggcggca acgagatcga cacccagggc gacgcgttct
     4501 tctactcgtt tgcccgggcc cgggccgcgg ttgccgcggc ggtggaggtc cagcgcgccc
     4561 acgtcgaaca ggagtggccg cgcggcgtaa aggtcaacgt gcgcctgggg ctgcacaccg
     4621 gtgagccggt cgtcggcgac gaggggtata ccgggctcga cgtcgttcgc gcggcgcgca
     4681 tcgcggcgga tggcaagggc ggccagatcc tgctgtcaga ggcgacccgc gcgatcgtcg
     4741 gcggcaacct gcccgacggc gtggcgatcc gcgagctggg tgagcggacg ctcaaggaca
     4801 tcgaccagcc cgagccgctg ttcgagctcg tctacgcccc cgagccagcc gccgcggccg
     4861 aatctgccct cgccgttgcg ccgagcgaga tctcgttcga tgtcaagccg gcgagcgtgc
     4921 tcaagcagtg gcagaagctg gcccgggtgg ccatcgacgc ggcgcgctac gacgaggatc
     4981 ccggcagcgc catcgaggaa cgcgtcctgg cgcaactcaa ggaaacgttc ggagacgaga
     5041 tcatcaccac cggcagcaac cccaaggtca agccgcccaa ggcgcccaag ctccacgacg
     5101 tgcctggcag gaagggcgtg ccggccaagt cggtcaagtc gggtgaatcg gtggccgccg
     5161 ggctcacccg cttcaacgaa ctgcgcgaag cgggcgcctt gaccgatgag cagtactcca
     5221 aggcggtcga tcggctgctg tcagagggct gacgctatgc tcgccgcgcg atgaccgacc
     5281 aaccactccc cgatcgaccc gtcacgggcg aagccaccga cccacacgca tcggccgatc
     5341 caacctggag acccgaaacc ctggcagtcc atgccggcat ggcgcccgat gagctgactg
     5401 gcgcggtcgc gccgccgatc taccagacag cgactttcgc gcaggaaggc gtgggccggc
     5461 cacgcggcgg ctgggagtac gcccgcaccg gcaacccgac gcgggcccgg ctcgagcagg
     5521 ccatagccgc gctcgaaggt ggcacgcacg gccttgcctt cgcttctggg tcggcaacga
     5581 cccaggtcat tgccacgctt gccctgcccg gtgagcggat cctgtgcgtg aacgacgtct
     5641 acggcggcac gttccggctg ttcgagcgcg tgctgcgcga caccggcgtc gacgctacat
     5701 atcacgactt cgcgggcgac tctggcggcg aacttgagcg caacctcgac gtgcggacgc
     5761 gcctggtctg gctcgagacg ccgaccaacc cgcacctgcg cgtgatcgac atcgcggcga
     5821 tcgcggcgcg gctggcgacg cacgtcggcg cccgcgggga gcgacccatc ctcgtcgtcg
     5881 acaacacctt cgcctcgccg ctgtcgcagc agccgctcaa gctgggcgca gacgtcgtat
     5941 tccactccgc gaccaagtac ctcgcgggtc actccgacac gatcagcggc gtcctcgcga
     6001 catcgcgcga tgacctcttc gcgcgcctct cgttcctgca gaacgcgatc ggcgcggtcc
     6061 ccgggccaat ggactgcttc ctcgtgttgc gcggcctgcg caccctcgcg ctgcgcatcg
     6121 aacgccacgc gtcgaatgcc caggcggtcg cggaggcgct ggccaagcgc gacgacatcg
     6181 agatcctcaa ctaccccggg ctgcgcagcg gccgctttgc ccatcccaac gcagatctcg
     6241 cgaagcgcca gatgcgctat gcgggcggca tggtctcgtt cgtgcccgcc gcgcgcgacg
     6301 gccgctcggc ggacgagcgg gcgcgcaact tcgtcgagcg gacgcgcatc ttcaccctcg
     6361 ccgaatcgct gggcggtgtc gagtcgctgt gcgagatccc ggcggtcatg acccatgggt
     6421 ctgtcgcgaa ctcgccgctc gaggtatcga cggcgctcgt ccggctatcg gtcggcgtgg
     6481 agcacgtcga cgacctgatc gccgacgtta cccaggccct ccaccaagcc tgacatcaag
     6541 agttcgtcac atcaagagtt cgtcaggtgc cggtctgcgc gtgtggcgcc gcgacagact
     6601 tggactcggg cgatcccttc tggcgcagga agatcgacag gacgatcaga acgccgactg
     6661 ccaggaactc gctttgccag ttctggagtg actcgaacca gaactgggag gtgaacaggt
     6721 actgagcggc cgtcacaggt ccgcttccgt gcgccagctg ctcttcgctg aacctcgcgg
     6781 ccccagtgat caagtgcatc acgaacgaga aggcaaagaa agcgaacagg gcaatcgaca
     6841 gcgaatggcg gtagatcgcc agcaccaggc caccacggcg cacgggccac ggcgcatcac
     6901 gcttggtgcc tttcctggtc gcgtcgtcag ccgcgccttt cgattcgtct ggatccttcg
     6961 actctgacga tccgcgctgg aagaggaata cggtgagcag cacgtaggcg cccatctgga
     7021 ggaactcgct ctcccagttc tcgaacatgg cctcaccgaa gtccggcgtc gcgaggtagt
     7081 ccgcgagccc tatagccagc cgaccgtgct cctcctgctc agcgttgaag gtgtcccagc
     7141 caacgaccgc cagcccgacc gcgcacaagg cgaagatcgt gaacatggcg atcgaaagcc
     7201 cgttgtcccg taagaactgc ttcatcacgg caatcgtggg cgccaggccg tcaagagtca
     7261 gtcgcgcaac ggctccagac atttcaaccg ctggacggtc gccgcctatg ctgcgcgatg
     7321 catggaccct tggcagacga tcatcgagcc gttccggatc cactcggtcg aaccgatccg
     7381 gatgaccacc gtggctgagc gcgaggccgc actcgccaag tccggctgga atctcttcaa
     7441 cctccatgcc gacgatgtga tcatcgacct gctgaccgac tctggcacag gtgccatgag
     7501 ccgcgaccag tgggcggcca tccagcacgg tgacgagtcc tacgccggat cgccttcgtg
     7561 gttccgcttt ctcgccgccg tccagaagct gtggcccttc aagcacgtga tcccgacgca
     7621 ccagggccgc gcggcggaga agatcctgtt cagcgtcatc ggcggcccgg gcaaactcgt
     7681 gcccaacaac gcgcacttcg acacgacgcg cgccaacgtc gagaacaccg gggccgaggc
     7741 gatcgacctg accccgccgc gcaacccgca cgagatgctg ccgttcaagg gcgacatgga
     7801 cgttgccgcg ttggagcggc tgatcaagga gcgcggcgtc gagaacatcc cggtcgtgtt
     7861 catgacgatc accaacaact cgatgggcgg ccagccggtg tcactggcca acctgcgcgc
     7921 ggtgcgcgag gtgtgcacgc gctacggcct gccgctcttc ttcgacgcgt gccgctttgc
     7981 cgagaacgcg tggttcatca agcagcgcga gcccggctac gccgccaaat cgatcacgga
     8041 catcgtgcac gagatggcgg ctctcgccga cggcatgacg atgagcgcaa agaaggacgg
     8101 cctggccaac atcggcggct ggctggcgat gaacgacgac gcgctcgccg agcgctgccg
     8161 caacgtgttg atcctgaccg aaggattccc gacctatggc ggcctcgccg ggcgtgacct
     8221 ggaggcaatc gcccaggggc tcgaggaagt cgtggaagag gactacctgc gctatcgcat
     8281 ccagtcgacg gcgtacctgg gtgaagcgct cgatgcttcc ggggtgccgg tgatcatgcc
     8341 catcggcggt cacgcggtgt acatcgacgc gcgcgagatg ctgccccaca tcccgccgct
     8401 gcagtacccc ggccagtcgc ttgccgtggc gctctaccgc gccggcggaa tacggggctg
     8461 cgagatcggc acggtcatgt tcgggatgca cccagatggg accgagacgg ccgccgcgca
     8521 ggatctcgtg cggttggcca tcccgcgccg cacctacacc cagtcgcaca tcgactacac
     8581 gatcgaggtc atccagcgcg tcgccgaaca ggcctcacag ctgcgcggct acaagatcgc
     8641 cagcgccccg gcagcgttgc gccacttcac cgcagttttc gcgccgctct aggcccctga
     8701 ttcgcatcgc ggggtgcggt cggggtagga tttgggggct gcgcgacggg agccgtgaac
     8761 ccgtccgcat agggactcgc atagagggac ttgtgcgaaa gcgtcttgcg ctgctggcgc
     8821 tacctgtgat ggtgctgtgc ctgttgctgc ccggccaggc aatcgccacg ccgtcgttca
     8881 gcacgaccgt cgagcgtgac acctgtgtcg cgggcgcggg caagtggggc ttcggccagg
     8941 gtgtcctgcg gatacgcatc atcgagtacg gcaagtcggg cgccaatcgg ttcacgttca
     9001 ctgcccaggt ctggcatctc ccactccacg gctctacgtg gaccaaggag catgagtggg
     9061 cacttggcgt ttacaccttc cgggacaaca accagtccta ctacaactca cgccagtacg
     9121 cgtgggcacc caaccacaac gcctaccaca agatcgtgat ccgggtccgt gcgctacgcg
     9181 acaacaccgt cctgtattcc aagctgttcc agggcaagac gtgctgacgc gcctggcacg
     9241 caacgtgcgc cgcgccggcc tgctgagcct cgcgctgctt gctttcatag cgcccgcgcc
     9301 gacacaggcc gacccaggtg acttcgtgtt cgaggtgctg cgcaaccgct gcgtcaacac
     9361 cggcaaggac ttcggccacg gagaggtgct gctcaaggtc aaggtgaagg agaacggcgc
     9421 gtcaggcgcc aacaagttca cccttgcggc tgttgcccag cacctcaagc ccagcaccgg
     9481 tacgtgggtc aacgagtaca cgttcgactt cgtcaaggtc acgttccccg acgacgcgaa
     9541 cagctactac cacgagcgct ggttcgcgta cgaccccaag gagagcaagg agcaccggat
     9601 cgtgctcgtg atgaagaccc tgcataacaa gcaggtgctt gcctcgcgga ccgtcaccag
     9661 caagagctgc tgaccgcgct attgcgccgg ctgcgtcccc aggtgacgcg ccacgaagtc
     9721 gatctccgcc gccacctggc ggatccgctc gtcggtgcgc agcgagccgt ggcccgcgtc
     9781 gaagcgcagc acctcgtgcg gcttgccaag ctcgaccagg cggtcgaggt agttgtcgat
     9841 ctggcgaatg gggcagcgcg ggtcgttggc gccggcgagg atgaacaccg ggacgcgcac
     9901 ctgctcgatg aacgtgagtg gcgagcgatc gacgtatgcc tggaggttgt cggtgggtga
     9961 gcccccgaag agcgcgtcgt cgtacgcctt gagcggcgcc atctcgtcct cgtaggccgc
    10021 gacatagtcg gcgactggca cgcccgcgac gccgagcgtc cagcgctcag cctgcaggct
    10081 gaggcccaga agggtcaggt agccacccca cgaattgccg ctcagcacca tgcgcgccgg
    10141 gtccgcgatg ccctgggcca cgaccgcgtc gaacacggcg gcgatgtcct tcagctcggt
    10201 cagcccggga ttgcccgtca gcgcgtcgcg ccacgcgcgg ccgtgacctg tgctgccgcg
    10261 gtagttgacc tggaccacgg ccagcccgtg atcgacccaa gcctggacga cgggctggaa
    10321 cgagtcgcga tcgtgcgcct cggggccgcc gtggatcgtg aagattgtcg gatgcggccg
    10381 cggcgtggtg ggctcagcaa tgaatgccgg cacgtcacct gccagcaggt cggtgtagcg
    10441 cacgccaccg ggtgactcct cgccgggcgc gcgcaggacg accccaccgg cgctgccatc
    10501 ggcgcgcacc gagcggatct cgggcggcgt tgccgcgtca ctccacaggt accacacctc
    10561 accatcgggt cgcgcggcgg cagaggagac cgtccccggg gcgatcggca gctcgacgag
    10621 acgctccggc tccagctcga ggcgatagag cgaggaccgc ccggcgtgct cgtggaccag
    10681 cagcagcgcg ttgccatcgg gataccacga cgcgtcgatc tcgcccggca ggtcgatctc
    10741 gaaggtgcgc gtccggccgt cgcgcggcca ccacagcatg ggtcggtgaa ggtccgcccg
    10801 ttcgtggctg accagcaccc gctcgtcatc ggccgcctgc gagaaccccg atgactcgag
    10861 gcccagccct gggccatccc acaggtcgcc gatgacagtc ccatccatgt ccacgacacg
    10921 gattgcggcg tggcgcgtgt caccgtgctc ggcgtggtgg aagcagagca actgctcgtt
    10981 ggccgacagc ccgcccagcc acgactcctg ctcgttggcg tagatctgac gcgcctcacc
    11041 accgaggacg tgaatggtgc tgccactctc ggtcgagcca ccgaccaccg ctacctcgcg
    11101 gccgagctgc aggccggtgc tgtagaagcg gccgagctcc tcaccggcca cggtgcccga
    11161 agtcgcgtcg aacggctgga cgatccaccg gccgaactcg tcaccgtccg tgtcgtcgaa
    11221 ccaccacacg ttctcgccgc gcggcccgat gtgaccgtcg atggtcccct cgcgccgatc
    11281 ggtcagctgg tgcgtcgtgc cggccgcgcg gtcccaggcg aacagctcga aacggccgac
    11341 gtggttggcg gcatagacaa gccgctcagg cgcctctgtc gcccactccg gcagcgtcgt
    11401 gcgcggcgcc cggaaccgcc gccgccatgg ggcttcaata gtggccggtg tcgtttctgg
    11461 catggcccgt gattatgatc ggcggcgtgg cagaggaggt cggcgcggcc ggcggacaga
    11521 cccgtggctt tctcttcgcc gacctgcgcg gctatacaga cttcgtcgaa aggcgcggcg
    11581 acgacgccgc cgccggtctc ctggagcgct acaggacgct cgtccgtggc gtcgtagcgc
    11641 gccacaccgg ggccgaggtc aagaccgaag gcgactcctt ctacgtcctc ttcccgtcgg
    11701 ccagctccgc cgtcgcagcc ggcctggaca tcgtcgcgag cgcggccgcg gaatcagccg
    11761 agcatccgga tgcaccgatt cgggtgggcg tgggcgtgca cgccggcgag acttccgtcc
    11821 taccggaggg gccggtcggc tccgcggtaa acatggccgc gcggatctgc gcccaggcgg
    11881 gcgccggcga ggtgctggtc tcggacacgg tgcgcggcct gactcggacg cgcaacgtcg
    11941 cggtcttcgc caacgtcggc agccgcaggc tcaagggcat cgcggagccg gtggcgctgt
    12001 atcgcgctgc gcctccggga acgatcatca agggtggcag ccgacgtccg actagcaagg
    12061 cattgcccgc gtcatgggtc ggtatcggtc tggcgatcct cgtgctcgtg cccgccacgt
    12121 ggatcatcgc gacggtgctc ggaagcgccg gtgccagccc cactccggcc gcgtcgaacg
    12181 catcgcccac cgcgccgtca gcggcaacgc ccacgaacgc aacgtctccc acggcagtgc
    12241 ctgtcacggg aacgcggatc atctatagca ggcagattgg ggccgacgga tgtggtggcg
    12301 tgccactcga tgcgaagctg catgttctcg accccgacca tccggctgtc gcaacctacc
    12361 gcgtatcgga tcgctccaac ctcctcgaga tcgaagccgc ctggtccgcg gacggctcca
    12421 cgatcggcct ggtgggtagc gtcggccgtg gcccgagcgg cctattcacc atcgatgcgt
    12481 cgggggcacc gctcaacgtg ctcgtcgcac cgctgtcccc tcaggcgctc aacccgttct
    12541 cgcccccgaa cgtcacggtc gcgcccgacg ggagtgccgt cgtacacatg gacgaggacg
    12601 cgctgtggcg cacgccgacc gacggttcgg gcaggacgca ggttgcaggc gtcatccgac
    12661 ccgaagaccc cgaactggaa ccgccaccag ctgtcgagcg gtatcgcgat gtcgtgtacc
    12721 ggtccgacgg aaccctgctc gtgctgatca tcggggtgga tggcggaacc ccatggctgg
    12781 agacgatggg cgacgatgga tccaatcgca ccagggtcga gctcgatctc gagggtctcg
    12841 atgttggcgg gatctcgctc gccgcggatg acgacaccct cgcgctactt gtacccagcg
    12901 agctaggcag ctcgatccag gtcggtcgga tcagctcggg catctcggac ttcgaggtgg
    12961 tcgactttga cgccgagaat cccagccagc ctgctttgtc gcccaccggc tccgagctcg
    13021 ttgtgtcggg cgattcgaac ggaaccgagc aactgtatgt agccgatctc gccagcggcg
    13081 aggtcaccca gctgacggac gatcctgatg cgtcagcttg ctcgccggcg tggcgcgaag
    13141 cgccaccgga tctgttggcc gggcgaccgg ctctcgaacc gggtgccggc ggcccctttg
    13201 agctcggcca gctgccgagc gggccggtac tcaacgaggt cgtcgatccg ccgattcagc
    13261 tgacgtttcc cgacgggtgg ttcgcgcggc gcaactacgt cgacggcttt tccgttttcc
    13321 gacccggcga ggtttatggc gagatcgact acggacggct ccagttcaac tcgagcggct
    13381 cctgcgacga tacggaactg gtagcggtcg ggcccctccc aaacgacctg ctgtccaact
    13441 tgcaggcacg aagcgatctg gagctggctg ctgtcggtcc gatcaacctg ggcgggtact
    13501 ccggcataac ggccgacatc agcggcaaac cagaacaggg ctgcggggaa cctgagtttc
    13561 ggtactggtc cctgttcagg actgctcagg atggcttcag cctctcggag gacgagcacc
    13621 tccgtctcgt tgcgctggac gtccgtggga gcactcgctc gttcctgata ttcgctcttg
    13681 cgccggacat cgacagctat tggaccgaaa aggcccggcc gatcctcgaa acggtggtgt
    13741 tccccgaggg ctgaggctac gcgggctttg gccccgcgtc gcggattgcg tccgacacgc
    13801 caccctcgaa ctgcttgaag ttctcgacga acatgccggc cagcttgcgc gcggcggcgt
    13861 cgtaggcgtc cttgtcggcc caggtatcgc gcgcccagag gacttcggca ggcacgtcgg
    13921 ggcaagtgac cggcacctcg aagccaaacg tcgggtccgt ccgcgtcggc acgcccttga
    13981 gcgccccgcg tagcgccgcc cgaaccatct gccgcgtcca ggtgatgttc atccgctccc
    14041 cagtgccgta aggcccgccg gtccagccgg tgttgaccaa ccagacgggc acgttgaagc
    14101 ggtccatgcg ctcgcccagc atctccgcgt agacgctcgg gtggcgcggc atgaacggcg
    14161 caccgaagca cgtcgagaac gtcgcggtgg gctccttgat gccgacctcc gtgccggcga
    14221 gcttggccgt gtagccgctg atgaagtggt acatcgcctg gtcgcgggtg aggcgcgaga
    14281 tgggcggcag gacgccgaac gcgtcggcgg tcaggaagat gatggtcgac gggtggccgg
    14341 ccatgccgac atcggagctg ttgctgatga aatgcaacgg gaacgcggcc cgtgtgttct
    14401 ccgtgaactt ctccgagtcg aggttgagct cacgcgtcag cggatcgatg tcgacgttct
    14461 cgaggatggt cccaaagcgc tgcgtggtcg tgtagatgtc gggctcgtag atgtgcgaga
    14521 ggcggatcgt cttggcgtag cagccgccct cgaagttgaa gacgccgtcg tcgctccagc
    14581 cgtgctcgtc atcgccgatg agcacccgct ccggatcggc cgacagtgtg gtcttgcccg
    14641 tgcccgacag cccgaagaag atcgccacgt cgccgtcctt gccgacgttg accgacgagt
    14701 gcatgggcag cacgccctca gtcggcaggc ggtagttcat gatcccgaat gcgcccttct
    14761 tcatctcgcc cgcgtactcg gtgccgcaga tcaggatctc ctgcttggcg aggtgcacca
    14821 ggatcaccgt ctcgctgcgc acaccgtccc gggccgggtc ggcgtggaac gagggcgcgt
    14881 caatgatcgt gaagttgggc tcgaagccga cgaggtcctc tgccttgggc cggatgaaga
    14941 ggttgtcggc aaagatgctc gcccaggcgc tttccgtgta gacgcgcagc gagcggcggt
    15001 agcgcggatc ggcgccgacg aacccctcct ggacgaagac ctcgcgctcg ttgacgtggg
    15061 acatgacgcg cgccttgagg tcggcgtagc gctcctcgct gatgggcgtg ttgaagccgc
    15121 cccaccacac gttcgcgtgc gcgtcgggct cgtcgacgag gtacttgtcc ttgggcgagc
    15181 ggccggtgtg cttgccggtg cggacgacga gcggcccgtt ggccgccagg atgccctcgt
    15241 cacggcggac ggcgtgctcg tacagctgtg ccactgagag gttcgagtgg cggatcttgc
    15301 ccagcagcgg catatcgctc gtgcgcagcg cgcgcttggt gggcttatcc agggtggtcg
    15361 ccacgaggcc ggttctcctt gctcagctga gcgcggcggg gattgcccgc gctcgttctg
    15421 gcggaccggc cgcgcgtgcc cggcacagcc gcaggctgca tccgaggagt cggctttcgc
    15481 agttcgtcgg gcgcattgta cgcggagccc ccagcggcaa gcgcgcgtaa ctggcagcgt
    15541 gctgcgcaac agttacgcgg caaaacgcaa ccgatgccaa tatgcgcggg ttacatgaac
    15601 gtgtgagtga catgaccttg aacagctcga actcgtcgcg cctcgactgg ctgctcttcc
    15661 tggtgctggg cttcatgtgg ggcagcagct accttttcat caagatcggc gtcgaggccg
    15721 gcctgccgcc gctcaccctg gtcatggcgc ggctgattat tggcggcgca ctgctgttgg
    15781 ccgtcgtgcg cctggcgcgc gagcccttcc ccaaaacgtt cagggagtac gccaagctca
    15841 gcgtgctcgg ctttttcggc atcgccctgc ccttctatct gatcaccgtc gccgaggcga
    15901 gcaccgattc ggcgctggca gccgtcctcg tctcgcccgt gccgctgttc gtgatcccca
    15961 tgtcggcgct gttcctgcac gacgagaagc tcacgccggc gcgcatcctg ggcgtggtcg
    16021 tgggtctcgt gggggtggcg atcctggtgg gcttcgaccc ggcgtcgctg gccaagaacg
    16081 actttggcgc cgagctgttg ctcgtggcgt cggccatttc ttatgccgtc gccggcgttt
    16141 atgccaagaa atacatcgtg ggctaccggc ccatgatccc ggcgctcttc gaggtcgcga
    16201 ccgcgctcgt gatgatcggt gtcgcggcgt tcatcttcga gcggccgtgg gaggcgccga
    16261 tcaacgtcga ttcggtggtt tcggtcgcgt ggctgggcct gttcggctcc ggctttgcgt
    16321 ttctcatctt ctttcggctg atcagcaact ggggcgccac gcggacgtcg acggtcgcgt
    16381 acctgatgcc cgtcgtgggc atcgcgctgg gtgcgctctt cctgtccgaa gagataactc
    16441 ccaaccggtt gctcgggacc gcgctggtaa tcggtggcgt ggccctcgtc aacgtcaagc
    16501 gcgaggccat cgcgagtgcg tgggccggct ggcgtggccg caccgcggac gaatcagcgg
    16561 aggtagccgc ggaggtcgtc tccgaccagc gctaggcacc cacctacaat cagctccatg
    16621 agcaccacga ccggctcgcc cgcccacggc gccgccacgc agccgatcag cctgcgcacc
    16681 cgattctccg cttgggcgga gatcatgcag actgccaacg ctcctatcga caagccgctc
    16741 gacaccatcg gcaagtggct ggtcatgacg cgcgccgcgg ttttccccat gacgctgtgg
    16801 tcgggcacga tcggcgcatt gctggccgtc gagcaggcgc gggtgacggg cgctgtttcg
    16861 atcgactggc tggccgtggt tctggcagtc gtgggcatcg tgctcgccca cgcggccaac
    16921 aacctgatca acgacttctt cgaccagtcg cagggcatcg acagcgagga ttacgttcgc
    16981 gcgctctacg cgccccaccc ggtgctgagt ggctggatca cgcgcgcggg gttgcgcaac
    17041 gccatcctcg cgctgaccgc catcgacggg ctgatcctga tcgccttggc gctccgcaat
    17101 ccgcagcccg cactggtggt cgccttcgca ctcgccgggc tcttcctgag cgtcttctac
    17161 gtcgctccgc cgttcagcct aaagcggcgc ggcctgggcg agctcgacgt cttcctgacg
    17221 tggggcccgc tgatgatcgg cggcacgtac ctcgtcgcca ccggcacgat ccccgcgtgg
    17281 gtgatcgtct tctccatgcc ctacgccctt ctcgtgacga gcgtcctctt cggcaagcac
    17341 atcgacaaga tcgtgccgga cacggcgctc ggcatccgga cgctgcccgt gatcctgggc
    17401 gagccactgg cccggcgcac cggccagctg ctgatgatcg ccttctatcc gctcctcgcc
    17461 ggcgccgtgc tggccggctg ggtgggtccg tgggtgctgc tcgcggggct ggcggtcccc
    17521 gagctaattc gcgcgctgcg cttcttc
//
LOCUS       DATBRX010000100         9644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_254728, whole genome shotgun sequence.
ACCESSION   DATBRX010000100 DATBRX010000000
VERSION     DATBRX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254728"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VIF08_03630"
     CDS             <1..381
                     /locus_tag="VIF08_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY8135106.1"
                     /translation="PTAMARGQMAAQRRALRKRLIDYALGLPEAYIDHPWGEDVAKVG
                     KKVFVFFGTGQGDFGMGVKLPDSAPLALAQQGVRPSGYGLGDSGWVNVNFSEATLPYE
                     MLRDWITESYRAVAPKKLSMTLEG"
     gene            626..2092
                     /gene="proS"
                     /locus_tag="VIF08_03635"
     CDS             626..2092
                     /gene="proS"
                     /locus_tag="VIF08_03635"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY8135107.1"
                     /translation="MTEKKSRFVSDITPQSEDFPGWYNDVVLKTEMADYTPVRGSMVI
                     RPYGYAMWESLRDQLDRYIKRTGHENVYFPLFVPKSLLEKEAEHVQGFNPQVAWVTHA
                     GGEELNEWLAIRPTSEAIIAEKVKDWVHSYRDLPLLYNLWNNVVRWELRTRLFLRTAE
                     FLWQEAHTFHATEQEAVDEVAVGLEGYRQACEDWLAIPVIRGRKTESEKFPGAVYTDT
                     IEALMRDRKALQCGTSHMLGQNFARAAGIEFLDRDNKLQHPYGTSWGFTTRMIGATVM
                     VHGDDSGLVLPPNLAPTQLVIVPIFRTEDDRRVVAEAVESVQKSLADVMTASGPLRVK
                     VDGREESPGFKYSHWEMRGVPYRLEIGPRDVPAGNGVLVRRIDREKQTVALDALAAEL
                     PTRLADYQAMLFQRAVDFRDANTHTADTYDEFKATLEREGGFLLAPWCGSAECEKKIN
                     ADTSATIRCIPFDSPDEAGKCMVDGKPSERRVLFARAY"
     gene            complement(2098..2835)
                     /locus_tag="VIF08_03640"
     CDS             complement(2098..2835)
                     /locus_tag="VIF08_03640"
                     /inference="COORDINATES: protein motif:HMM:NF016365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEY8135108.1"
                     /translation="MFLTGTLLNVAAVLIGTTVGVLAGSRVPERMQRTLTDGLGLFTI
                     AIGIALALRYLTDPALPMGDDLAVLGALLAGGAVGELLRLSDRLDALGDWFQRKLARG
                     DQPSRVSEAFVTASLVFCVGPLTILGSIQNGLTGDIQLLGVKSLLDGVASIAFAAALG
                     AGVYLAALTVLVVQGGIATLAWAAGAGLDPGSIAAASAVGGLMLVGVGLGLLDVKRIR
                     VVNMLPGLVFAPLFVWLAAAIRGALAP"
     gene            complement(2826..3788)
                     /locus_tag="VIF08_03645"
     CDS             complement(2826..3788)
                     /locus_tag="VIF08_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135109.1"
                     /translation="MKGSRGPAKGLDSQALDWLVASDEPGIRMQARRDLLGQKVSIPA
                     DEILAGPMVRRLLAGQKADGGFGGHPYHKWTGVHWRLVSLVELGVPAGTPAVTAALDR
                     DLDWMLVDGRPTARMRIAGVQRVHASMHANPIACAVRLGMADDTRVRQLVGWLIDGQW
                     ADGGWNCDRHPAASHSSFYESVLALAALAEFARATGDAGAAQASRRAADFFLEHRVFK
                     SHTADRPGDPKWQKLRYPEYWHYDYLHGLVMLMRAGALPDARAQDALDLLREQQQPDG
                     RWVTAGAQYWRGTTGTYGDPASWNASSASQMLTLNALRVLKACS"
     gene            3953..5320
                     /gene="nusA"
                     /locus_tag="VIF08_03650"
     CDS             3953..5320
                     /gene="nusA"
                     /locus_tag="VIF08_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY8135110.1"
                     /translation="MNREALGALLQLNAEKNIPREQLVASLAEAIESAYRRATPGNEN
                     VYVEFDEPSAGFKAYRGMMVVEEVLDPQTEWTVEEAAKTKPDAKVGDLIKLGDIDTNQ
                     MGRVHAQIAQQLFRQRLREVEREQVFGQYAQRESEIITGTVSRVEPRGVILDVGRAEA
                     VLPVTDQSATEHYRIGQHVKAYVLEVRRTTKGPLIYVSRTHKNFLRRLFELEVPEIHG
                     GTVEIKAIAREAGSRSKVAVFSRQEGLDPVGATVGQRGGRVQAVVAELGGEKIDIIPW
                     HEDPAVFVANALSPAQVVRVDIDEENRIASVTVPERMLSLAIGREGQNARLAAKLTGW
                     RIDIRSDQAPPRVAPAAAVSTEAPAEQVVATEPAAVAEVPAAAAEETPVKPKRATKAK
                     AAAAEKPEKSATAKKSATAKKASPKASERTAVAKPKAAPKAKAAPKEKAAAKPKAASK
                     KAAKE"
     gene            5317..5610
                     /locus_tag="VIF08_03655"
     CDS             5317..5610
                     /locus_tag="VIF08_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEY8135111.1"
                     /translation="MTQATAHALPARHFPTRTCVACRTERQKREFVRVVRAPDGSVSI
                     DSTGRANGRGAYLCADRTCWPDAIKKKSIERALGVSLPADVRAQLEGGVGHGS"
     gene            5630..7495
                     /gene="infB"
                     /locus_tag="VIF08_03660"
     CDS             5630..7495
                     /gene="infB"
                     /locus_tag="VIF08_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8135112.1"
                     /translation="MRGPRKPPRRTGDGGGSTAIQTAEPTARGAVEIPNSITVKDLAD
                     LFGVNPADVIRELIKSGIFATINQLIDRDTASLVAGELGFEVSESAVPEQAEAKNGKE
                     PPTPQATKEVLFEEDDPSLLQPRPPIVTVMGHVDHGKTSLLDAIRTTTVATGERGGIT
                     QHIGASEINRDGRRVVFLDTPGHEAFTAMRARGARVTDIAVLVVAADDGVMPQTLEAI
                     DHAKAAQVPIVVAVNKIDKPEANAERVKTELSEHGVIIEEFGGDTPLVPVSAKTGAGL
                     QDLLDTILIVADVQELKANPKRPAIGTVVEAELDKGRGPVATVLVQTGTLKVGDIVVV
                     GDTYGRVRALENSHGQRITKAGPSSPAVILGLAEVPAAGDILRVVADEKAARTMIETR
                     RAGAAPEQTGRATLEDLYRQIQAGQTKELRVILKADVQGSLGAIRHALEQVQNDEVRI
                     NILHEGTGDITDNDILLASASNAIVVGFNAKVDPQARRTAEAEGVDVRLYDIIYQLTD
                     EMRAALSGLLEPVEVEVVEGRAEVRQIFRSGRTVICGSYVTEGRIVKGGARVYRGGKL
                     IATDKIESLRRFRDDVREVQTGYECGIGLADFNSAEEGDVIECFSQQMVSRVVEK"
     gene            7506..7922
                     /gene="rbfA"
                     /locus_tag="VIF08_03665"
     CDS             7506..7922
                     /gene="rbfA"
                     /locus_tag="VIF08_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY8135113.1"
                     /translation="MTNRTRRLDELLREEINEVIRRDVDDPRIGFHTITDVEVAPDLR
                     HATVWVSVIGSPDEKKQTLRALGHAMPYVRGRLGRLRLKRIPEFHVREDETAARGTRV
                     MKILEELEQGGTGELEPSIETLPSPQRKSPAAEGDG"
     gene            7919..8971
                     /locus_tag="VIF08_03670"
     CDS             7919..8971
                     /locus_tag="VIF08_03670"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEY8135114.1"
                     /translation="MTVNSPNSPNSLNSLTLADVAAVPADIVQRLLAATDVLTVCHEN
                     PEADALGSALGLALALETLGKRATPLCSDVVPPMYEFMPHIDRFRRDPEAGHDYDLIV
                     VGDCGDLARVGSVLGRHGDLFGRVPIVNIDHHISNTGFGALNWVDATAAATCEMDTLL
                     MPALGVPLDAVNGAIAANLMAGVVIDTANFQHPNVTPRTLRVAAELVGAGAPLSDTAR
                     LLYRTKPNRQLKLFGLVLGRIESELDGRLTWSALYDSDYEASGASVEDTEGLSDLLSQ
                     SASAEMMLLFRDMGERTKISVRTRDGGPDATVLTGLFGGGGHARASGATLPQPIDAAI
                     PVVLDAARRELTERRG"
     gene            8964..>9644
                     /gene="truB"
                     /locus_tag="VIF08_03675"
     CDS             8964..>9644
                     /gene="truB"
                     /locus_tag="VIF08_03675"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8135115.1"
                     /translation="MAEADAPKRGGGGATPFDGIIVVAKEPGPTSHDIVALVRRLTGV
                     KRVGHGGTLDPFAAGVLPVFVGQATRLVEYHMADDKAYRALVAFGARSTTDDLDGELT
                     PTGAAPTRAELEAALASFRGEIDQVPPDYSAVRVGGRKAYELARHGEKPELRSRRVTI
                     HRVDLLAWDDADPARPVATIEVACSAGTYIRAIARDLGDQLGSGAYLAALTRTASGPF
                     TIDAAHALD"
BASE COUNT         1683 a         3324 c         3308 g         1329 t
ORIGIN      
        1 ccaaccgcta tggcgcgggg ccagatggca gcgcagcgcc gcgcgcttcg taagcgcctg
       61 atcgactacg ccctcggctt gccggaggcg tacatcgacc atccgtgggg cgaggacgtc
      121 gccaaggtcg gcaagaaggt attcgtgttc ttcgggacgg ggcagggtga cttcgggatg
      181 ggcgtgaagc tgcccgattc ggcgcccctg gcgctggccc agcaaggagt caggcccagt
      241 ggctacggcc tgggcgactc cggttgggtc aatgtcaact tcagcgaggc gacgctccct
      301 tacgagatgc tgcgcgactg gatcaccgag tcataccgcg ccgtcgcacc caagaagctg
      361 tccatgacgc tcgaaggctg aggggcgccc ctcgtgacgc agctatggtc atggagagcc
      421 ctaacggcgc tcatcgtgcg ctgatcacgc tgcggcgagt cggcgagggg tccttcccag
      481 cgtgaccctg agctcgccac atccctgcat gtgggccaaa tggccgcgcg tgcatctcga
      541 agcggcttcg tgaccatagt ggagtcacgc gcgacgacaa acaacggtcg gctcggcagc
      601 gacgctgcgc tagcattcgc gccccgtgac cgagaagaag agccgtttcg tctccgatat
      661 caccccgcag tcagaagact tcccgggttg gtacaacgac gtcgtgctca agaccgagat
      721 ggccgactac acgccggtgc gcggcagcat ggtcatccgg ccgtacggct acgcgatgtg
      781 ggaatccctg cgcgaccagc tcgatcgcta catcaagcgg accggccacg aaaacgtcta
      841 cttcccgctt ttcgtgccca agagcctgct cgagaaggaa gccgagcacg tccagggctt
      901 caacccgcag gtcgcgtggg tgacgcatgc cggcggcgag gagctgaacg agtggctggc
      961 cattcgaccg acgagcgagg cgatcatcgc cgagaaagtc aaggactggg tccactcgta
     1021 ccgcgacctg ccgctgctct acaacctctg gaacaacgtc gtgcgctggg agctgcgcac
     1081 gcggctcttc ctgcgcaccg ccgagttcct gtggcaggaa gcgcacacct tccacgccac
     1141 cgagcaggag gcggtcgacg aggtcgcggt cggtctcgag ggctatcgcc aggcgtgcga
     1201 ggactggctc gccatcccgg tcatccgcgg ccgcaagaca gagagcgaga agttccccgg
     1261 tgccgtctac acggacacga tcgaggcgct catgcgcgac cgcaaggcgc tccagtgcgg
     1321 gacaagccac atgctcggcc agaatttcgc cagggcggcc ggtatcgagt tcctcgatcg
     1381 cgacaacaag ctccagcacc cgtacggcac gtcatggggt ttcactaccc gcatgatcgg
     1441 tgccaccgtg atggtgcacg gcgatgactc cggcctggtg ctgccgccca atctcgcgcc
     1501 gacccagctg gtcatcgtgc ccatcttccg gaccgaggat gaccggcgcg tcgtagccga
     1561 ggcggtcgag tcggtgcaga agtcgctggc cgacgtcatg accgcgtcag ggccgctgcg
     1621 cgtcaaggtc gacgggcgcg aggagtcgcc cggcttcaag tacagccact gggagatgcg
     1681 cggggtgccg taccggctcg agatcgggcc gcgcgacgtg ccggccggca acggcgttct
     1741 ggtccggcgc atcgatcgcg agaagcaaac agtggccctt gacgcgttgg cggctgagct
     1801 gcccacgcgc ctggccgact accaggccat gctcttccag cgggcggtgg atttccgcga
     1861 cgcgaacacc cacacggccg acacgtacga cgagttcaag gccaccctcg agagggaagg
     1921 cggcttcctc ctggcgccgt ggtgcggcag cgcggagtgc gagaagaaga tcaacgccga
     1981 caccagcgcc accattcgct gcattccctt cgactcgccc gacgaagcgg gcaagtgcat
     2041 ggtcgacggc aagccgtccg agcgacgggt cctcttcgct cgcgcctact agcgaactta
     2101 cggcgccaac gccccgcgga tggccgcggc gagccacacg aagagcggcg cgaagaccag
     2161 ccccggcagc atgttgacga cgcgaatgcg cttcacgtcg agcaggccca gtcccacgcc
     2221 cacgagcatc aggccaccga ccgcgctcgc ggcggcgatg gagcccggat cgaggcccgc
     2281 tccggccgcc caggcgaggg tcgctatccc gccctgcacg acgagcaccg tcaacgccgc
     2341 gaggtagacg ccggcgccga gcgccgccgc gaaggcgatc gaggcgacgc cgtcgagcag
     2401 gctcttgacg ccgagcagct ggatgtcgcc tgtcagcccg ttctggatgg agccgaggat
     2461 cgtgagtggg ccaacgcaga acacgaggct ggcggtcacg aaggcctcgc tgacgcgcga
     2521 cggctggtcg ccgcgggcca gcttgcgctg gaaccaatcg cccaaggcgt cgaggcggtc
     2581 ggacagacgg agcagctcgc ccacggcgcc gccggcgagc agtgcgccaa ggactgcgag
     2641 gtcatcgccc atgggcagcg ccggatcggt cagatagcgc agagcgagcg cgatcccgat
     2701 cgcgatcgtg aagagcccga ggccgtcggt cagggtgcgc tgcatccgtt cgggcacgcg
     2761 cgagccggcg agcacgccga ccgtcgttcc gatcaacacc gcggcgacgt tgagcagcgt
     2821 gcccgtcagg aacacgcctt gagcaccctc agcgcgttga gcgtcagcat ctggctggcc
     2881 gacgacgcgt tccagctcgc cgggtctcca tacgtgccag tcgtgccgcg ccagtactgc
     2941 gcgcccgccg tcacccatcg cccatccggc tgctgctgct cacgtagcag gtccagcgcg
     3001 tcctgggcgc gggcatcagg cagggcgccg gcgcgcatca gcatcacgag gccatgcagg
     3061 tagtcgtagt gccagtactc cgggtaccgc agcttctgcc acttgggatc gccgggccgg
     3121 tcggcagtgt gcgacttgaa gacgcggtgc tcgaggaaga agtcggccgc gcggcgtgac
     3181 gcctgcgctg ccccagcgtc gccggtcgcg cgagcgaact cggccaaggc cgccagcgcg
     3241 agtaccgatt catagaacga cgagtggctc gcggcgggat ggcggtcgca gttccagccg
     3301 ccgtcggccc actggccgtc gatcagccag ccgacgagtt gccggacgcg cgtgtcgtcg
     3361 gccatcccca agcgcaccgc gcaggcgatc gggttggcgt gcatggaagc gtgaacgcgc
     3421 tgcacgccgg cgatgcgcat gcgggcggtc ggcctgccat cgacgagcat ccagtccagg
     3481 tccctatcca gggcggcggt caccgccggt gtccctgctg gcacgccgag ctcgacaagc
     3541 gagaccagcc gccagtggac gcccgtccac ttgtggtaag gatggccgcc gaacccgccg
     3601 tcagccttct gcccggccaa caggcgccgc accatcggcc ccgccaggat ctcgtcggcc
     3661 gggatgctga ccttctggcc gagcaggtcg cgccgcgcct gcatgcggat gccaggttcg
     3721 tcggacgcca caagccagtc gagggcctgg gaatcgaggc ccttggcagg cccacgggag
     3781 cctttcatcg ttcgcttcag gttacaggcc cgggacggct ggccgaagcg gctataattc
     3841 ggccggtata tttcttggcc gcctctgaac gtgggtgacc cccacgttta tttatgcgga
     3901 ggggacacct cgccaacggg ggaagcctgg tcgaaagaaa ggagggcgct cgatgaaccg
     3961 tgaggctctt ggcgcgctcc tgcagctcaa cgcggagaag aacatcccgc gcgagcaact
     4021 ggtcgccagc ctggctgagg ccatcgaatc cgcctaccgg cgcgctaccc cgggcaacga
     4081 gaacgtctac gtcgagttcg acgagccatc ggctggcttc aaggcatacc gcggcatgat
     4141 ggtggtcgag gaggtgctcg acccccagac cgagtggaca gtcgaagagg ccgccaagac
     4201 caagccggac gccaaggtgg gcgacctcat caagctgggc gacatcgaca ccaaccagat
     4261 gggccgcgtc cacgcccaga tcgcccagca gctattccgc cagcgcctgc gcgaggtgga
     4321 gcgcgagcag gtcttcggcc agtacgccca gcgcgagtcg gagatcatca ccggcaccgt
     4381 gtcgcgggtt gagccgcgcg gcgtgatcct cgacgtaggc cgggctgagg cagtgctgcc
     4441 cgtcaccgac cagagtgcga ccgagcacta ccgcatcggc cagcacgtca aggcttatgt
     4501 gctcgaggtg cggcgcacga ccaagggccc gctgatctac gtcagccgga cacacaagaa
     4561 cttcctgcgc cgcctcttcg agctcgaggt acccgagatt cacggcggca cggtcgagat
     4621 caaggcgatc gcccgcgaag cggggtcgcg ctccaaggtg gccgtcttta gccgccagga
     4681 gggcctcgat ccagtcggcg caacggtcgg ccagcgtggc ggccgcgtcc aggctgtcgt
     4741 ggccgagctc ggcggcgaga agatcgacat catcccgtgg cacgaggacc cggcggtctt
     4801 cgtcgcgaac gcgctcagcc cggcgcaggt cgtccgcgtc gacatcgacg aggagaaccg
     4861 catcgccagc gtgaccgtgc ccgagcggat gctctcgctg gccattggcc gcgagggcca
     4921 gaatgcgcgc ctggcggcca agctgaccgg ctggcggatc gacatccgct ccgaccaggc
     4981 accgccgcgc gtcgctccgg ctgccgcggt ttccactgag gcgccggccg agcaggtcgt
     5041 ggccacagag cccgccgccg tcgccgaagt gcctgcggcc gccgcggaag agaccccggt
     5101 taagccaaag cgcgccacca aagcgaaggc ggccgctgca gagaaaccgg agaagtccgc
     5161 aacggcgaag aagtccgcga ccgccaagaa ggcctccccg aaggcgagtg aaaggacggc
     5221 cgtcgccaag cccaaggccg cgcccaaggc aaaggccgcg cccaaggaga aggccgccgc
     5281 caagcccaag gccgcgtcca agaaggcggc gaaagaatga cccaggccac cgcccacgcc
     5341 ctgccggccc gccacttccc cacgcgcacc tgcgtggcgt gtcgcacgga gcgccagaag
     5401 cgcgagttcg tgcgcgtcgt tcgcgcgccc gacggcagcg tgagcattga cagcaccggc
     5461 cgcgccaatg ggcgcggcgc atacctgtgc gctgaccgga cgtgctggcc ggacgccatc
     5521 aagaagaagt cgatcgagcg cgcgctcggc gtcagcctgc cggccgacgt gcgggcgcag
     5581 ctcgaggggg gagtcggaca tggctcgtag tcggagcggt acacgcaaca tgcggggccc
     5641 gcgcaagcca ccgcgccgga ccggtgatgg tgggggctcg acagcgatcc agaccgccga
     5701 gccgactgcg cgcggcgcgg tcgagatccc gaactcgatc acggtcaagg acctggccga
     5761 tctcttcggc gtcaatccgg ccgacgtgat ccgcgagctg atcaagagcg gcattttcgc
     5821 gacgatcaac cagctgatcg atcgtgacac ggcgagcctc gtggcagggg agctcggctt
     5881 cgaggtctct gaatcggctg tgcccgaaca ggccgaggcc aagaacggca aggagccccc
     5941 caccccgcag gcgaccaagg aggtcctctt cgaggaggac gacccgagcc tgctgcagcc
     6001 gcggccgccg atcgtgaccg tgatgggcca cgtcgaccac ggcaagacga gcctgctcga
     6061 cgccatccgc acgaccacgg tcgcgacggg cgagcgcggc ggcatcaccc agcacatcgg
     6121 cgcgagcgag atcaaccgtg acggccggcg cgtggtcttc ctcgacaccc ccggtcacga
     6181 ggccttcacg gcgatgcgcg cccgcggcgc gagggtcacg gacatcgcgg tgctcgtggt
     6241 ggctgccgat gacggcgtga tgccccagac gctcgaggcc atcgaccacg ccaaggccgc
     6301 gcaggtgccg atcgtggtcg cggtcaacaa gatcgacaag ccggaggcaa acgcggagcg
     6361 cgtcaagacg gagctgtccg agcacggcgt catcatcgag gagttcggcg gcgatacgcc
     6421 gctcgtgccg gtcagcgcca agaccggcgc cgggctgcag gacctgctcg acaccatcct
     6481 gatcgtcgcc gacgtacagg agctcaaggc caaccccaag cgcccggcca tcggcacggt
     6541 ggtggaggca gagctcgaca agggccgcgg gccggtagcc acggtgctcg tccagacagg
     6601 cacgctcaag gtgggcgaca tcgtggtcgt aggcgacacc tacggccgcg tgcgcgccct
     6661 cgagaacagc cacggccagc gcattaccaa ggctggccca tcgtcgccgg cggtgatcct
     6721 gggtctcgcc gaggtaccgg cggcgggcga catcctacgc gtcgtcgccg acgagaaggc
     6781 cgcgcgcacc atgatcgaga cgcgtcgcgc cggcgccgcg cccgagcaga cgggccgcgc
     6841 cacgctggag gatctgtacc gccagatcca ggccggacag accaaggagc tgcgcgtcat
     6901 cctcaaggct gacgtccagg gctcgctcgg tgccatccgc cacgcgctcg agcaggtcca
     6961 gaacgacgag gtgcggatca acatcctgca cgagggcacg ggcgacatca ccgataacga
     7021 catcctgctc gcctccgcgt cgaacgcaat cgtcgtcggc ttcaacgcca aggtcgatcc
     7081 acaggcccgg cgtacggctg aggcagaggg cgtcgacgtg cgcctgtacg acatcatcta
     7141 ccagctgact gacgagatgc gcgccgcatt gagcggcctg ctcgagccgg tcgaggtgga
     7201 ggtcgtcgaa ggccgcgccg aggtgcgcca gatcttcagg tcgggtcgga ccgtgatctg
     7261 tggctcgtat gtcacagagg gccgcatcgt caagggcggc gcgcgcgtct accgcggggg
     7321 caagctcatc gcgaccgaca agatcgagtc gctgcgccgc ttccgtgacg acgtgcgcga
     7381 ggtccagacg ggctatgagt gcggcatcgg cctggccgac ttcaactcgg ccgaagaggg
     7441 cgacgtcatc gagtgcttca gccagcagat ggtctcgcgc gtcgtcgaga agtaggtctt
     7501 aggcggtgac caaccgaacg agacgcctcg acgagctgct gcgcgaggag atcaacgagg
     7561 tcatccggcg cgacgtcgat gacccgcgca tcgggttcca cacgatcacc gatgtcgagg
     7621 tggcgcccga cctgcgccat gcaaccgtgt gggtgtcggt gatcggcagc ccggacgaga
     7681 agaagcagac cctgcgcgcc ctgggccacg cgatgccata tgtccgcgga cgcctgggcc
     7741 ggctgcgcct caagcgcatc cccgagttcc atgtccgcga agacgagact gcggcgcgcg
     7801 ggacgcgcgt catgaagatc ctcgaggagc tcgaacaggg cggcacgggc gagctcgagc
     7861 cgtcgatcga gacgctgccg tcgccccaac ggaagagccc cgcggccgag ggcgacgggt
     7921 gacggtcaac tcgcccaact cgcccaactc gctcaactcg ctcacgctgg cggacgtcgc
     7981 cgccgtgcca gcggacatcg tgcagcgcct gctcgccgca accgacgtgc tcactgtgtg
     8041 ccacgagaac ccggaggcgg acgcgctcgg ctccgcgctg ggtttggcgc ttgccctgga
     8101 gacgctcggc aagcgggcca cgccgctgtg ctccgacgtt gtgccgccga tgtacgagtt
     8161 catgccgcac atcgaccgtt tccggcgcga tcccgaggcc ggccatgact acgacctgat
     8221 cgtggtcggc gactgcggcg acctggcccg cgtcggatct gttttgggtc gccacggcga
     8281 cctgttcggc cgcgtgccga tcgtcaacat cgaccaccac atcagcaaca ccgggttcgg
     8341 cgccctcaac tgggtggacg cgaccgcggc ggcgacgtgc gagatggaca ccctgctgat
     8401 gcccgcgctc ggcgtgcctc ttgacgccgt gaacggcgcg atcgccgcga acctcatggc
     8461 gggcgtggtc atcgacacgg ccaacttcca gcatcccaac gtgacgccgc gcaccctgcg
     8521 tgtcgcggcc gagctggtcg gcgcgggtgc gccgctgtcc gacaccgcgc ggctgctcta
     8581 tcgaaccaag cccaaccgcc agctgaagct cttcggcctg gtgctgggcc ggatcgaatc
     8641 ggagctcgac ggcaggctta cctggtcggc cctgtacgac tccgactacg aggcctcggg
     8701 cgcctcagtc gaggacaccg aggggctgtc cgacctgctc agccaatccg ccagcgcgga
     8761 gatgatgctc ctcttccgcg acatgggtga gcgaacgaag atcagcgttc gcacccgcga
     8821 cggcgggccc gacgctaccg tgctgaccgg cctcttcggc ggcggcggcc atgcccgcgc
     8881 atcgggcgcc acgctgccac agcccatcga cgcggcgata cccgtcgtgc tcgacgccgc
     8941 gcgtcgcgag ctgaccgagc ggcgtggctg aggcagacgc gccaaaacgc ggcgggggcg
     9001 gggccacacc gttcgacggc atcatcgtgg tcgccaagga gccgggaccg acctcacacg
     9061 acatcgtggc cctggtgcgc cggctgacag gcgtgaaacg ggtgggccac gggggcacgc
     9121 tcgatccgtt tgccgcgggc gtgctgccgg tgttcgtggg ccaggcaacg cggctcgtgg
     9181 agtaccacat ggccgacgac aaggcgtacc gcgcgctcgt cgccttcggc gcgcgatcga
     9241 ccaccgatga cctggacggc gagctaacgc caaccggggc cgctcccacg cgggcggagc
     9301 tcgaggcggc gctggcgtca ttccgcggcg agatcgacca ggtgccgccc gactactccg
     9361 cggtgcgtgt cggtgggcgc aaggcgtacg agctggcacg ccacggcgag aagcccgagc
     9421 tgcgctcgcg ccgcgtgacc attcatcggg tcgacctgct cgcctgggat gacgccgatc
     9481 cggcacgacc ggtggccacc attgaggtcg cctgctcggc cgggacgtac atccgcgcca
     9541 tcgcgcgcga cctgggcgat cagctgggca gcggggcgta cctggcggcg ttgacacgta
     9601 cggccagcgg cccgttcacc atcgacgccg cacatgcgct cgac
//
LOCUS       DATBRX010000101         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2713093, whole genome shotgun sequence.
ACCESSION   DATBRX010000101 DATBRX010000000
VERSION     DATBRX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713093"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VIF08_03680"
     CDS             complement(<1..199)
                     /locus_tag="VIF08_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135116.1"
                     /translation="MATLRTAHSDELTAAEVARLHELMRDAFADNEHGGFTDDDWQHA
                     LGGTHLLLEADGAIVCHASVVT"
     gene            complement(218..1237)
                     /gene="cydB"
                     /locus_tag="VIF08_03685"
     CDS             complement(218..1237)
                     /gene="cydB"
                     /locus_tag="VIF08_03685"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEY8135117.1"
                     /translation="MITLADIWFVLIGVLLVGYAVLDGFDLGVGMLSPLVARTSAQRS
                     TLMQAIGPVWDGNEVWLLTLGGALFAAFPLVYATVFSGFYLALMLLLLGLIMRAVAME
                     FRHQVEWPRWQRAWDWVWWLGSLLPALLLGVALGNVARGLPLDDGQYLGGLLGLLNPF
                     SLLVGAAGVAMFATQGAAWLVLRTVGEVQARARRLGTYCWAAFAGLWVLVTIAAVIDA
                     PHLWVNYGQPLLWLAPLAFVAAMAWTGWSLLRGGSDRSPIAGSSLAIGALILILGQAL
                     YPALLPDRAGSADLTVSNAASTDTSLTVMLVVALIGMPLVLGYTVFVYRRFTHKVEES
                     SPGYH"
     gene            complement(1234..2616)
                     /locus_tag="VIF08_03690"
     CDS             complement(1234..2616)
                     /locus_tag="VIF08_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY8135118.1"
                     /translation="MDAVALARLQFAFTAAFHFIFPAITIGLGLLVAIAETIRWRRND
                     DTWDRLARFWTRIFALTFAVGVATGIVMEFQFGTNWSRFSTFVGDIFGSPLAAEGIFA
                     FFLESTFLGILLWGRERISSRLRWFSALMVSVGSALSGLWIIIANSWMQTPAGYEIHG
                     DRAVLTDFLAAALNPSTLPRYLHTIAAATAVGAFAMAGIAAWYLLRQRHQDVARLSLK
                     LGLSVALVSSLAMFITGDTHSRQVADTQPVKFAAMNGLFDTEQGVPMIIFAFPPGDDG
                     PSDLPAVAIPNLLSLLTRFDANATIDGLREYPRDVWPPVAATFTTYHLMVGLGTLMLL
                     VMVLAVLAWRRGRLDRWRWLLGAIVLMTPAPLIAVQLGWATAEIGRQPWIVYGLMRTA
                     DGVSPIVSAPEVLLSLIGFGLVYLVLAALWLFLVRRELMTGPAPGAIVPEPEQQIESV
                     PAIVPAEVPA"
     gene            complement(2764..>3246)
                     /locus_tag="VIF08_03695"
     CDS             complement(2764..>3246)
                     /locus_tag="VIF08_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEY8135119.1"
                     /translation="ATPRPTASPTPAPTPTPAPKDYSGRSHFWYPALNIDADWSWYGC
                     DYGGDPNGLGGGIYRWGCGPANNIYLLSHAWSTFKKIKLAYHNGALQEGQNLWYATAQ
                     GDVTKWEVKWIRRVTDDYLDATAGEWALNDSPTPIMTLQTCDGSQDQYRIIVRLVPAD
                     "
BASE COUNT          625 a         1060 c         1109 g          452 t
ORIGIN      
        1 gcgtcaccac ggacgcgtgg cacacgatcg cgccgtctgc ctcgaggagg aggtgcgtgc
       61 cgcccagggc gtgttgccag tcgtcgtctg tgaagccacc gtgctcgttg tcggcaaagg
      121 cgtcgcgcat gagctcgtgc agccgcgcca cttccgcggc cgtcagctcg tcactgtgcg
      181 ctgttcgaag ggtcgccacc cggcgacgat agcgaggtta gtggtagccc ggcgatgatt
      241 cctccacttt atgcgtgaag cgccggtaca cgaacacggt gtaccccagg acgagcggca
      301 ttccgatcag ggccacgacc agcatcaccg tcaggctggt gtccgtggaa gcggcattgg
      361 agactgtgag atcggccgac ccggcccgat cgggcaggag cgccgggtac agggcctggc
      421 ccaggatcag gatcagcgcg ccgatcgcga gcgacgagcc cgcgatgggg gagcgatcag
      481 agccgccgcg cagtaaggac cagccggtcc acgccattgc cgccacgaag gccagcggcg
      541 ccagccacag cagcggctgc ccgtagttga cccagagatg gggcgcgtca attaccgccg
      601 cgatcgtcac caggacccac aacccggcaa atgccgccca acagtaggtt cccagtcgac
      661 gtgcccgcgc ttgcacctcg cccaccgtgc gcagcaccag ccatgcggcg ccctgagtcg
      721 cgaacatggc gactccggct gcgccgacga gcaggctgaa cgggttgagc aggccgagca
      781 ggccgcccag gtattggccg tcgtcgagcg gcaggccacg tgcgacgttg cccagcgcca
      841 ctccaagaag gagcgctggc aagagcgagc ccagccacca cacccagtcc caggcgcgct
      901 gccaacgagg ccattccacc tggtggcgga actccatggc cacggcgcgc atgatcaagc
      961 cgagcagcaa caacatcagc gccaggtaga agccggagaa cacggtcgcg tagaccagtg
     1021 ggaacgcggc gaacagcgcg ccgcccaggg tcaacagcca gacctcgttg ccgtcccata
     1081 cgggcccgat cgcctgcatg agcgtcgagc gctgagcgga tgtgcgggcg accagtggtg
     1141 acagcatccc gacaccaaga tcgaagccat cgagcacggc gtagccgacc agcaggactc
     1201 cgatcagaac gaaccagata tcggcaaggg tgatcacgcg ggcacctccg ccggcacgat
     1261 cgccggcacc gattcgattt gctgctccgg ttccgggacg atggcgcccg gtgccggccc
     1321 ggtcatgagc tcgcgtctca ccaggaacaa ccacagcgcg gccagcacga ggtagaccag
     1381 cccaaatccg atcaacgaga gcagcacctc gggcgcgctc acgatgggcg acacgccgtc
     1441 ggcggtgcgc atcagtccgt acacgatcca cggctggcgg ccgatctccg cggtcgccca
     1501 accgagctgc acggcgatca acggcgcggg tgtcatcagg acgatcgctc cgagcagcca
     1561 gcgccaccga tccagccgcc cgcgccgcca ggcgagcacg gcgagaacca tgaccagcag
     1621 catcaacgtg cccagcccga ccatgaggtg ataggtggtg aatgtcgccg cgacgggtgg
     1681 ccacacgtcg cgcggatact ccctgagccc gtcgatggtc gcgttggcat cgaagcgggt
     1741 gaggaggctg agcaggttgg gaatcgccac agcgggcagg tcggacggcc cgtcgtcgcc
     1801 gggcgggaag gcgaagatga tcatcggcac accctgctcg gtgtcgaaga ggccgttcat
     1861 cgcggcgaac ttcaccggct gggtatcagc cacctggcgc gagtgggtgt cgccggtgat
     1921 gaacatcgct agtgacgaga cgagggcaac cgacaggccg agcttcaggc tgagtcgtgc
     1981 cacgtcctgg tgacgctggc gcaggaggta ccacgcggcg atgccggcca tggcgaaagc
     2041 gccgacggcg gtcgccgcgg cgatggtgtg caggtagcgc ggcagggtcg agggattgag
     2101 cgcggcggcc aggaagtcgg tcaggaccgc gcggtcaccg tggatctcgt agccggccgg
     2161 cgtctgcatc cacgagttgg cgatgatgat ccacaggccc gacagcgctg aacctacaga
     2221 caccatgagc gcgctgaacc agcgcaggcg tgagctgatg cgctcccgcc cccagagcag
     2281 gatccccagg aaggtcgact cgaggaagaa cgcgaagatc ccctcggccg cgaggggcga
     2341 tccgaagatg tcgcccacga acgtcgagaa acgcgaccag ttggtcccga actggaactc
     2401 catcacgatg cccgttgcca cgcccacggc gaaggtcagg gcaaagatgc gtgtccagaa
     2461 gcgcgcgagg cggtcccagg tatcgtcgtt gcgtcgccag cgaatggttt cagcgatcgc
     2521 gacgagcagg cccaagccga tcgtgattgc cggaaagatg aagtggaaag ccgccgtaaa
     2581 ggcgaactga agacgcgcga gagcgacggc gtccaacggg ccacctccga ctcacagggt
     2641 ggacggccgg gcaacgctcc ggccggaatt accaccgtcc gacgttagcc agggagatgc
     2701 ccgacgacat gtgccgaagg tcgcaaagtc agccgcgcca cggcctgtgg cagcgactca
     2761 ggatcagtcc gcagggacga ggcggacgat gatccggtac tggtcctggc taccgtcgca
     2821 ggtctggagc gtcatgatcg gcgtcggtga gtcgttgagc gcccactcgc ccgcggtcgc
     2881 gtcgaggtaa tcgtcggtca cgcgccggat ccacttgact tcccacttgg tcacgtcgcc
     2941 ctgcgcggtg gcataccaaa ggttctggcc ttcctgcagg gcaccgttgt ggtaagcgag
     3001 cttgatcttc ttgaacgtgc tccaggcatg gctgagcagg tagatgttgt tggccgggcc
     3061 gcaaccccag cgatagatgc cgccgcccag gccattcggg tcgccgccgt agtcgcagcc
     3121 gtaccacgac cagtcggcgt cgatgttcag ggccgggtac cagaagtgcg atcgcccgct
     3181 gtagtccttt ggcgcaggcg tcggggtcgg agccggtgtc ggcgaggcag ttggtcgcgg
     3241 cgtcgc
//
LOCUS       DATBRX010000102        19920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2713127, whole genome shotgun sequence.
ACCESSION   DATBRX010000102 DATBRX010000000
VERSION     DATBRX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19920
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713127"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VIF08_03700"
     CDS             <1..187
                     /locus_tag="VIF08_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135120.1"
                     /translation="RVSLVDGAPGIVSIDGRDDYPIEVGDSVEVRAMDRPMRFIEPPG
                     ALPFWDLLRQKAALLPA"
     gene            317..1990
                     /gene="recN"
                     /locus_tag="VIF08_03705"
     CDS             317..1990
                     /gene="recN"
                     /locus_tag="VIF08_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY8135121.1"
                     /translation="MLRELAVRDLALIERARVELGAGLTVITGETGAGKSLLIDALGL
                     VMGARADSGLVRHGATGARVEALFDRAGAADNDGATDDPLICVREVSAEGRSVARIDD
                     ETVTAARLAATVDPLVEIHGQHDQQRLLQGAWQRDVLDAYGGCGELRLAVREAVQALR
                     SNEAALRELATDPAELQRRLELAAFAADEIEAASVQPGEINELKSRLALAGNAQRIAS
                     LLSDAHEALSGEGRGAHDGLARASRAVADLARLDASAQSLSERMIGLEAELDDIALEL
                     RRRAEQMSETVGDTEQIEARLGVLYGLMRKYGDDEAALIEYGAQQRAEVDRLKGTEAE
                     RARRQADAERLGTAAHIAASALHNARAKTATGLAGEVTARLVELGFPSAAFVIDLDDA
                     PLDESGSDAVSYLLAPNPGEPPRPLARIASGGELSRVALALKSVLAAADQTPTLIFDE
                     VDAGIGGRSADPVGRLLWRLARDHQVLCVTHLPQIAAYADGHLRIEKRERDGRTVTEI
                     TRLDDGERRHELAQMLGGAAGATATLAAADELLERAASAREQLAPAASA"
     gene            2178..3101
                     /gene="xerD"
                     /locus_tag="VIF08_03710"
     CDS             2178..3101
                     /gene="xerD"
                     /locus_tag="VIF08_03710"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY8135122.1"
                     /translation="MTLDDAIEAYLTFLRVERGLADATMRAYANDLRGYGQYLERKRA
                     NWASGAQGAVDYLAALTKPPRVLKPSSHRRKAAAIRAFYRFCFAEGLTEKDIASLLDL
                     PRAARQLPDTLDVGQVEALLEAPSATTELGLRDRALLELLYACGLRVSEALNLDLEDV
                     ALSEGSVRVIGKGDRERRLPVGDVALAALTKYIDEARPALLGVAQHKQAKSALKRGGP
                     LFVSARGARLSRMAAWRVIRHAALVGGVRGHVTPHTLRHSFATHLLEGGADLRVVQEL
                     LGHASITTTQLYTHLTGERIRQVYARAHPRA"
     gene            3119..3871
                     /locus_tag="VIF08_03715"
     CDS             3119..3871
                     /locus_tag="VIF08_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY8135123.1"
                     /translation="MARYADTLLTEGEVIVLRTRQHWLALIGRARAGVALFVGGLLLL
                     FLIAYFNVAPGTMRDILSGVALIMLALGVIVFLYRFWIWWAQDYMITNRRLLKVTGVL
                     NKRSADSSLEKINDAILSQSLLGRMLNFGDLEILTAAEQAVDRYHMLRSPKEFKKTML
                     TQKHNLETEFIYGRAPTPPLRADAPAPAAPPAPMPAAPAPAPAAPAPAASEEDESREV
                     TETLARLADLRDSGAVTAEEYEAKKGELLGRL"
     gene            3868..4524
                     /locus_tag="VIF08_03720"
     CDS             3868..4524
                     /locus_tag="VIF08_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8135124.1"
                     /translation="MTPERLVGGAIVVAIFLLIAFPVHEFSHAWAAYRLGDSTARYQG
                     RLTLNPIAHFDPLGGGLLAVSMLFAGIGFGWAKPTPVNPYNLRYGRRGEAIVALAGPI
                     SNLVMAMAVAIPLRLIHADPALLVAVASNDAASFAFFVAVLFVKIAILLLVFNLLPIP
                     PLDGWRALLGLVDARTAYSLRQFEQYGFVVLILVIVAAPRFVGDLTSVLAGILLGFPV
                     "
     gene            4646..5032
                     /locus_tag="VIF08_03725"
     CDS             4646..5032
                     /locus_tag="VIF08_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135125.1"
                     /translation="MDLFDAMHPADQRHGLDVVAALRADGHAQPDLLLAGLLHDCGKG
                     RDLHVWHRVAWSLGERYGARVERAFERVPTFRAAFATMRDHAARSAELAAAAGCNAAT
                     VELIRNQAEPTDPVLGRALLLADQAN"
     gene            5051..5866
                     /locus_tag="VIF08_03730"
     CDS             5051..5866
                     /locus_tag="VIF08_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEY8135126.1"
                     /translation="MTAPATPVEISFGTGRRPEESTHVRSDYFDGPLALLLALIEQRQ
                     LDVLTVRLGDLAGAYLDALARIETGRLGLLSSFVTVCSQLILIKTRALLPRAPHLGAD
                     GTAADAADPEEELRRRLIEYRMFRDAGRVLGDRRATGAWLYHREAAAALAAGLSGARP
                     DPGPALAPQLLADALLASVKLVPPAPAPPEVMARTITLEERTEVIRAALKRAPLIVLQ
                     ELLRDVTDRVVVAVTFLALLELAKGREVAIDQAEPWGPITVTRLPAENQASDG"
     gene            5859..6446
                     /gene="scpB"
                     /locus_tag="VIF08_03735"
     CDS             5859..6446
                     /gene="scpB"
                     /locus_tag="VIF08_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY8135127.1"
                     /translation="MVDELTDLTDPADQPRGPLEPPPGELSAAQLEALLFVAERPLAR
                     REIASLAGVAVEDVDALLGDLEVTLRERGLRLLSAGEHVQLVTAPEAGRLIARYIGAD
                     GGRLSPAALETLAIVAYRQPVTRSVIERIRGVDSDYVLRSLLHRRLVVEQGRADAVGR
                     PFLYGTGFDFLERFGLTSLDELPPLEPEAALRLDE"
     gene            6439..7908
                     /gene="cmk"
                     /locus_tag="VIF08_03740"
     CDS             6439..7908
                     /gene="cmk"
                     /locus_tag="VIF08_03740"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY8135128.1"
                     /translation="MSDRVRVHKALADAGVASRRAAEQMVIAGRVAVNGVTATVGLQV
                     DPAADQITVDGKLISGAGQSRVYLLLAKPAGVTSTVSDRHAEQTVIDLVPAALRQGAA
                     RLYPVGRLDRDSEGLLLLTNDGAWAQRMLHPRHGVEREYAVGLLQPLTAEQREQLTDG
                     IILEEGVARLIALRQSTSAETRRLVALLGDQVRGLAWYQVTLGQGMKRQVRRMFGAVN
                     MPVHRLVRMRIGTLRMGDMALGDTRRLSAAERGQLELLVTGHGRDLVVSVDGPGGSGK
                     STVGMRAAEVVGYRFCDTGVLYRGLTWLQMARGVDVDDADALVGLIAHMRLEHDERQR
                     YVRLVADDREVTDELHTAEVDRSVSSVSQHAQVRAALLPVQRALADGGRLIMAGRDIG
                     TVVLPDADLKLYLDVSVAERARRRAAERGLANDPRALAQIEAELRERDEIDSTRAASP
                     LRIPDGAIVLDTSANTLEQTVDQVVALIRRAEKDDSLDE"
     gene            7901..8590
                     /locus_tag="VIF08_03745"
     CDS             7901..8590
                     /locus_tag="VIF08_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8135129.1"
                     /translation="MNDAVAKGPSKGVPLLLRIAGVLSRTLLRLGARVTVEVDGDIPR
                     TGPLIVVSNHISNADPPLVAGWLTPMLGRQMHILAKQALFIGPLGWILRKLGATPVKA
                     GGSDIEAYRVARAVLDRGDVLCIFPEGTRSVTGVMQEPKPGVAMLATRTGTPILPVGV
                     SGSDYFLGRGKKLPRLRAPIRLHIGKPFTLTLDPHLPHRQAMAAASDELMDRIAALVD
                     ERHRGRFGAAN"
     gene            8669..9280
                     /locus_tag="VIF08_03750"
     CDS             8669..9280
                     /locus_tag="VIF08_03750"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HEY8135130.1"
                     /translation="MTQAPDPNAMPGSSPPPDGPQPGPMGYQAPPSGPTSYMPPPVAP
                     PKKKTNWLGIVVLVAIVGAIGGGFWLFRDRLSSNASELVVGDCFDKPAETETITDVQH
                     QPCTSPHDREVITVLTHPAGPDDPYPVVSGFDDYIQENCVPAFETYTGRDADTESELN
                     LGYFQPTLSGWGEGDRGLSCFISRRDGQQLHGSLRNIGTSPLP"
     gene            9345..10331
                     /gene="ispH"
                     /locus_tag="VIF08_03755"
     CDS             9345..10331
                     /gene="ispH"
                     /locus_tag="VIF08_03755"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY8135131.1"
                     /translation="MGNVEEVRIARRTGFCYGVREAIDYARLAAAQGKQTHTLGQVVH
                     NEGVIAQLQAQGIQTVDTLDDVDRGAAVVIRAHGVTPEVMGRAQERGLEVIDGTCTWV
                     IQEQKELKSLVDDGYTIVLLGTPRHPEVVGLLGFAPDAIVVDEEEDWERIPRTKRMAL
                     ISQSTQPPWKFEKLAAYLVSRCHELKIINTVCPVTIRRQEDTMALAAEVDMMVVVGGR
                     NSANTKELTRLCQIANKPVIQIEGAPDLADGGAFGSARVVGVTGGTSTPIEDLEAVAE
                     RVFALAGTADRQPRAKELAHAALTEVATPAYRSTSLDEHGREKGHAPIAGAA"
     gene            10333..11859
                     /gene="der"
                     /locus_tag="VIF08_03760"
     CDS             10333..11859
                     /gene="der"
                     /locus_tag="VIF08_03760"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY8135132.1"
                     /translation="MPTRRGRRTPQTVERDAPTEGLPIVAIVGRPNVGKSTLFNRIVG
                     DRVAIVEDRARTTRDRLYDVGEWNGRRFVVVDTGGLETRPGDPIEERVQEQARVAIAE
                     ADTIVFMVDAEAGLTPADEEASSLLRTAKAPVIVAANKSDNAGREIQAAEFHAMGWPE
                     THPISAIHGRGVADLLDVIVWSLPPESESEVERKRAEDEASRDDAVTEGETEEAAFAV
                     GDRPVRLAIIGRPNVGKSSLLNALLGQPRAIVSDIPGTTRDAIDTTLEWAGRTMRLID
                     TAGVRRRGKVAAGPDAERFATMRALKAVTRADVAVLVIDAVDGMTSQDDHVAGYALDE
                     GTGLVIAINKWDLVEKDGHTFDDYVARLRRHAPFLAKTPILSISAMTGQRVERVLEAA
                     VEIAAERRRRIPTPALNTWLRDVTARRPPATVNGRQPRFLYATQVGSTPPTFVLFVSD
                     SKGVHFSYKRYLDNQLRAAFGFEGTPIRLVIRERERDEARRPRRSAARATRSKSRARG
                     "
     gene            11870..12931
                     /locus_tag="VIF08_03765"
     CDS             11870..12931
                     /locus_tag="VIF08_03765"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY8135133.1"
                     /translation="MERHATRAVVGAGQWGTTLALHLSRRGPVTLLARDEEQARQLIG
                     ERRNSRYLPDIALPVEIEITADPAALASASDLVIFAVPSRAMRTSAERVRGNLHNEAV
                     LLSVAKGLEQGSLDRMTQVLADVLPEAKERIGAMSGPNLALEIARGLPASAVIAAEDV
                     EVSRTASEIIGTRSFRVYRNRDVTGVELCGALKNVIAIAAGAAEQLGFGDNGKAGLIT
                     RGLAEMTRLGMAAGAHPLTFAGLAGLGDVIATCYSALSRNHALGVELAKGRKWSDIEG
                     TLAGVAEGAYTVEAALELAKRHNVEMPIAQEVHNGIYEGKSVQRCLIDLLSREQKDEL
                     ADVEQWARRAYGTKLSTGV"
     gene            13315..13391
                     /locus_tag="VIF08_03770"
     tRNA            13315..13391
                     /locus_tag="VIF08_03770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13349..13351,aa:Pro,seq:ggg)
     gene            13454..13771
                     /locus_tag="VIF08_03775"
     CDS             13454..13771
                     /locus_tag="VIF08_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135134.1"
                     /translation="MAREPAADGGKRLADSAVLLRRYRQHLRVVIGMLWDDYERRGTQ
                     ALNIELDSAHALEWDIQVRPIRAAHYRQAPVGYDKPAAFALGLDLNLLVGVKQLCKQL
                     VAH"
     gene            complement(13910..14788)
                     /locus_tag="VIF08_03780"
     CDS             complement(13910..14788)
                     /locus_tag="VIF08_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8135135.1"
                     /translation="MNRSLLYGMGIGVVVTAIVALLLGGAFIGPRATPSPSVRPSLSP
                     RPSASVRPSPTPIDTAIRATAVVVPQRSADLALPVSGIVDQIFVHELDAVTIGQLLLR
                     LDQSTYEAAIDVAQEELDSAEAAVTKAELQVERLPEDATPAEIEQAQVELRLARTDRD
                     VAASALSEAQVALRQTELRAPFSGTVAAINVERGEQAHAGEPVVTIGDLSGWLIETTD
                     LSELEVVHVHVGDAATVTFEALPDLVLAGHVDQIQVRGTGPAGNVVFAVAIRPDRHDE
                     QLFWNMTATVRITPSD"
     gene            14832..15272
                     /locus_tag="VIF08_03785"
     CDS             14832..15272
                     /locus_tag="VIF08_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEY8135136.1"
                     /translation="MAAVRSARAQWQGNLTEGSGSVSSLSSGQLVDLPISWSARTEDP
                     AGLSGPEELLAAAHASCFSMAFSNTLFKAGFTAEHIETTVEITGDKREAGWTVISSHI
                     TVRCRVPGASDADIADAAEKAKDGCPISRALKGNVELSVDATLE"
     gene            15287..15676
                     /locus_tag="VIF08_03790"
     CDS             15287..15676
                     /locus_tag="VIF08_03790"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEY8135137.1"
                     /translation="MPPIDHLQRVPIYHLEALERQGIFTTGLLLEVSETPTRRQYLAD
                     QISATVNDVHEWRDEALLLNLANFGPAEQRLFTQAGILGLSDLLALNAAEYEQRLARS
                     ARALGVELPPSLVVEGWMEQAHTLAEE"
     gene            15703..16350
                     /locus_tag="VIF08_03795"
     CDS             15703..16350
                     /locus_tag="VIF08_03795"
                     /inference="COORDINATES: protein motif:HMM:NF018964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HEY8135138.1"
                     /translation="MSWLLPPATALVIIVVGVLLLTTSIWMHFAIPAAGGGLPGYPIE
                     ASLVASDRTVRQLLGLGDFLISVECPGAAPCPPQELLYTADEQAHLRDARAVLYLFVA
                     LAVASLAFVIAALAHRPHDARRWRAVARGGAGLAIGAVVVGVVGYLVFDAAFELFHRV
                     LFPGGNWEFPADSNMIRLYPYAFWQLTAAALGMLSVIGGGLVWWLARRRAASLAE"
     gene            16347..17618
                     /gene="glp"
                     /locus_tag="VIF08_03800"
     CDS             16347..17618
                     /gene="glp"
                     /locus_tag="VIF08_03800"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY8135139.1"
                     /translation="MSHELPPLMSLEDARARMLEGLAALPTEMVALGDAAGRVLAVDL
                     TSRHTLPPWDNSAMDGFAVIAADVAMASKDAPVRLAVTGEAAAGRVADVAVTSGKALR
                     ILTGAPVPAGADAVVPVENTDARPGVADLPREVAIYSAAEPGAHIRRAGSDLTAGAPL
                     LEAGTELTPAAVAVAAAGGHAQLTVHRPPHVAVLATGDELVAPGQPLGPAQIPDSNTF
                     GVAAQARELGADVQVLGIARDDLDDVLAHLRQGLGWADVIVASGGVSVGAHDVVKDAF
                     AQIGRIDLWRIAVQPGRPLAFGRAPATDGQREVLLFGLPGNPVSSLVTFELFVRPVLR
                     RLAGQHDVIGRDIVRAALEQPVSKAHNRRAFIRVKLTAADDGLGWRASLAGGQDSHVL
                     SALAAADGLAIIGEEFDSVPAGTQVEVIRIR"
     gene            17615..18187
                     /gene="moaC"
                     /locus_tag="VIF08_03805"
     CDS             17615..18187
                     /gene="moaC"
                     /locus_tag="VIF08_03805"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY8135140.1"
                     /translation="MRQPPDRSERRRLTHVSRGGRPRMVDVSAKPATARRAVAEAFVT
                     LEQETLTAIIDGQTTKGDVLTVAEMAGVMGAKKTSELIPLCHPIALTDIVVEITPERA
                     GPGVRIRATAATVGPTGVEMEALTAASVAALTVYDMVKGVDRGAAIREVRLLEKSGGK
                     SGEWRRAPGDTAPRPGVPTKRGSFRKRPER"
     gene            18184..18681
                     /locus_tag="VIF08_03810"
     CDS             18184..18681
                     /locus_tag="VIF08_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY8135141.1"
                     /translation="MSKVALVLTISDGVSAGTRQDESGAALAARLTELGYGVERATVA
                     DEMDEIATAVSGATGHAALVISTGGTGLGPRDVTPQTLHGLLDYEIAGFGEQMRAFGR
                     TRTPMADLSRSVAGVLGTTLVIAVPGSRGGALDSLTAIEPLLDHALETLGGHTQHAAS
                     GEGKS"
     gene            18682..>19920
                     /locus_tag="VIF08_03815"
     CDS             18682..>19920
                     /locus_tag="VIF08_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY8135142.1"
                     /translation="MVERVPLYPLAPTLFILAVIGFVLLMARHMRVFALARPAPVGDQ
                     AGWFASMFKYAIVQIRMFRDLDAGLMHATIFWGFVILTVGTADRVTFGLIERIVRPLL
                     DGWLWRLLMLGQNMFVLGVLIMVGYALFRRLVWRYPRRTTLSRDALVILFLIGGVVLS
                     EWFAEGFRIAAYGDPDAGWAVMATPLSWVINGLLPAQVLGAWYALFFWMNVALVSFFL
                     IYLPHSKHLHIVTAFFNAALRKTRPRGELPQIDLEAADARFGVKTVADLSWKDLLDGF
                     TCTECGRCQEACPAWATGKPLNPKTMIMGIREMSVEAEAGVPLIPWIRPGGGTAIHGT
                     DKPIVDAAIPYDAVWDCVTCGACVEACPVMIEHVDKIVGLRRSLVLEDSRFPEELTAS
                     FNNLERYSNIWGQPQSTRLDW"
BASE COUNT         3081 a         6871 c         6906 g         3062 t
ORIGIN      
        1 gcgcgtcagc ctcgtcgatg gcgcgccggg catcgtcagc atcgacggcc gcgacgacta
       61 cccaatcgag gttggcgact cagtcgaggt gcgcgcgatg gaccggccga tgcgcttcat
      121 cgagccgccc ggagcgctgc ccttctggga cctgctgcgc cagaaggccg cgctgctccc
      181 cgcgtaatcg acatcgccga gtcgcgacgt cacgggggct gagtcaccct gcgtaactca
      241 ccagtcatca tggatgactc agcaccgttg actgagagcg cagcatcaac gccgccgcga
      301 gcgctaggct gacttcgtgt tgcgcgagct ggcggtccgc gacctggcac tgatcgagcg
      361 cgcccgcgtc gagctgggcg cgggcctgac cgtgatcacc ggcgagaccg gtgccggcaa
      421 atcgctcctc atagacgcgc tgggcctggt gatgggcgcg cgggcggaca gtggcctcgt
      481 gcgccacggc gcgacgggcg cgcgggttga ggcgctgttc gatcgcgccg gtgcggccga
      541 caacgacggc gcgaccgacg acccgctgat ctgcgtacgc gaggtgtcgg cggagggccg
      601 gagcgtggcg cgcatcgacg atgagaccgt cactgccgcg cgcctcgcgg cgacggtcga
      661 tccgctcgtc gagatccacg gccagcacga tcagcagcga ctgctccagg gagcgtggca
      721 gcgcgacgtg ctcgacgctt acggcggctg cggcgaactg cggctggccg ttcgtgaagc
      781 ggtgcaggcg ctgcgctcca acgaagccgc gctcagagag ctggccaccg atccggccga
      841 gctgcagcgc cgcctcgagc ttgccgcgtt cgctgcggac gagatcgagg ccgcatctgt
      901 acagcccggc gaaatcaatg agctcaagtc gcggctcgcc ctggcgggca acgcccagcg
      961 catcgcgtct ttgctgagcg atgctcatga ggcgctgagc ggggagggtc gcggcgcgca
     1021 cgatggactg gcgcgggcgt cacgcgcggt ggccgacctc gcgcggctgg atgcgagcgc
     1081 gcaatcgctg tccgagcgca tgatcgggct cgaagcggag ctcgacgaca tagcgctgga
     1141 gctgcgccgg cgggccgagc agatgtccga gaccgtgggc gacacggagc agatcgaggc
     1201 gcgcctgggc gtcctgtatg ggctcatgcg taagtacggc gatgacgagg cggcgctcat
     1261 cgagtacggc gcgcagcagc gggcggaggt cgaccgactg aagggcacgg aagccgagcg
     1321 cgctcgtcgc caggctgacg ccgagcgtct gggcaccgcc gcgcacattg cggccagcgc
     1381 cctgcacaac gcccgcgcga agacggctac gggcctggca ggggaggtga ccgcgcggct
     1441 ggtcgagctc gggtttccgt cggccgcatt cgtgatcgac ctcgacgatg cgcccctcga
     1501 cgagagcggc agcgacgccg tgagctatct cctcgccccc aatccgggcg agcctccccg
     1561 gccgctcgcc aggatcgcca gcggcggcga gctgtcgcgc gtcgcactcg cgctcaagtc
     1621 ggtccttgcg gcggccgacc agacgccgac gctgatcttc gacgaggtgg atgcgggcat
     1681 cggtgggcgc tcggccgatc cggtcggtcg gctgttgtgg cgcctggcac gcgaccacca
     1741 ggtgctgtgc gtcacccacc tgccgcagat cgccgcctac gccgacggcc acctgcgcat
     1801 cgagaagcgc gagcgagatg gccgcacggt gaccgagatc acgcgcctcg acgatgggga
     1861 gcggcgccac gagttggccc agatgctcgg cggcgcggcg ggcgcaacag cgacgctcgc
     1921 cgccgcggac gagctgctcg aacgtgcggc gtctgcgcgc gagcagctgg cgccggcggc
     1981 gagcgcctga ccttgttcgc caccgctgaa acaaaagcca agatgtcgcc cgatttgggc
     2041 cagatcaggc ccgagcctgg ctgcctgttc agcggcccta gacaaaccgc caccttcgag
     2101 gcccaagttt gccgttgttt cgctgcaggt gaacagacgc caccttgggg tatcgcggcg
     2161 gaggtgcagg cgaacccatg acgcttgacg acgcgatcga ggcgtacctg acgtttctgc
     2221 gcgtcgagcg cgggcttgcc gacgcgacga tgcgcgctta tgccaatgac cttcgcgggt
     2281 acgggcaata cctcgagcgc aagcgcgcca attgggcgag cggcgcacag ggcgcggtcg
     2341 actatctcgc cgcgctcacc aagccgccgc gcgtcctgaa gccgtcgagc cacaggcgca
     2401 aggcagcggc catccgggcg ttctaccggt tctgcttcgc cgaggggctg accgagaagg
     2461 acatcgcgtc gctgctcgat ctgccgcgcg cggcgcgcca gctgcccgac acgctcgacg
     2521 tcgggcaggt cgaggcgttg ctcgaggcac cgagcgcgac aaccgagctc ggcctgcgcg
     2581 atcgcgccct gctcgaactg ctttacgcct gcgggctgag ggtgagcgag gcgctcaacc
     2641 tcgacttgga ggacgtggcg ttatcggaag gcagcgtccg ggtcatcggc aagggcgacc
     2701 gcgagcggcg gctgccggtt ggcgacgtgg cgctcgcggc gctgacgaaa tacatcgacg
     2761 aggcgcggcc ggctctgttg ggcgtcgccc aacacaaaca agccaagtcc gcgctcaagc
     2821 gcggcggtcc gttgttcgtc agcgcccgcg gcgcgcgcct gagccgcatg gccgcgtggc
     2881 gcgtcatccg tcacgcggcg ctcgtgggcg gcgtgcgcgg ccacgtgaca ccgcacacgc
     2941 tgcgtcatag ttttgccacc cacctgctcg agggcggggc cgacctgcga gtcgtgcagg
     3001 aattgctggg acacgcgagc atcaccacca cgcagctgta cacccatctc actggcgagc
     3061 ggatcagaca ggtttacgcg cgggcccacc cccgcgccta ggaagaggag acacgcacat
     3121 ggcccgatac gcggacaccc tcctgaccga aggcgaggtc atcgtcctgc gcacccgcca
     3181 gcactggctg gcgctcattg gtcgcgcccg cgccggcgtc gcgctgttcg tgggcgggct
     3241 cctgctcctc ttcctgatcg cctacttcaa cgtcgccccg ggcaccatgc gcgacatcct
     3301 gagcggcgtc gcactgatca tgctggcgct cggcgtgatt gtgttcctgt accgcttctg
     3361 gatctggtgg gcacaggact acatgatcac caaccgccgg ctgctcaagg tcaccggcgt
     3421 actcaataag cgctcggccg acagctcgct cgagaagatc aatgacgcca tcctgagcca
     3481 gtcgctcttg gggcggatgc tcaacttcgg cgatctcgag atcctgaccg cagccgagca
     3541 ggcggttgac cgctatcaca tgctgcgtag ccccaaggaa ttcaagaaga cgatgctcac
     3601 ccaaaagcac aacctcgaga ccgagttcat ctacggccgc gcgccaacgc cgccgctccg
     3661 cgccgacgcg ccggccccag ccgccccgcc ggcgcccatg ccggccgcac ccgctccagc
     3721 gccagccgcg cccgcaccgg cggcctccga ggaggatgag tcgcgcgaag tgaccgagac
     3781 gctggctcgc ctcgccgatc tgcgcgacag cggcgcggtc accgccgagg aatacgaggc
     3841 gaagaagggc gagctgctcg gtcggctgtg acccctgaac ggcttgtcgg cggcgcgatt
     3901 gtcgtcgcca tcttcctgct gatcgcgttt ccggttcacg agttcagcca cgcctgggcg
     3961 gcgtaccggc tgggcgactc gacggcgcgt taccagggcc ggctgacgct caacccgatt
     4021 gcccactttg accccttggg cggtggcctg ctggcggtca gcatgctgtt tgccggaatt
     4081 ggcttcgggt gggcgaagcc gaccccggtc aacccctaca acctgcgtta cggcaggcgc
     4141 ggcgaggcca tcgtcgccct ggccgggccg atttcgaatc tcgtgatggc catggccgtg
     4201 gccattccgc tgcggctcat ccacgccgat ccggcgctcc tcgttgcggt cgcgagcaac
     4261 gacgcggcga gcttcgcttt cttcgtcgcc gtgctgttcg tgaagatcgc catcctgctg
     4321 ctcgtcttca atctgctgcc gatcccgccg ctcgacggct ggcgggcgct gctcgggttg
     4381 gtcgatgccc gcaccgcgta cagcctgcgc cagttcgagc agtacggctt cgtggtcctc
     4441 atcctggtga tcgtcgcggc accgcggttc gtcggcgacc tgaccagtgt cctggcaggc
     4501 atcctgctcg ggttcccggt ttgacggggc gcacgcgccg atgagttggt gggcggccaa
     4561 ggtccgtcgg tttgggcgct atttcaccgg ccgcgtcagc gcagcggagc gggccgaact
     4621 gcggagctgg ctttccccgc cgcagatgga cctgtttgac gccatgcatc cggccgatca
     4681 gcgtcacggg ctcgatgtcg tggccgcgct ccgggccgac ggccacgcac agcccgacct
     4741 gcttctcgcc ggtctgctgc acgactgcgg caaaggccgc gatctccacg tgtggcaccg
     4801 cgtggcctgg tcgctcggcg agcgctatgg ggcgcgggtc gagcgagcgt tcgaacgggt
     4861 gcccaccttc cgggcggcat tcgccacgat gcgcgaccac gccgcgcgct cggccgagct
     4921 ggccgcggcc gcgggttgca acgccgccac ggtcgagctg attcgcaacc aggctgagcc
     4981 gaccgacccc gtgctcggcc gagcgctgct gctcgccgac caggccaatt agggcgaggg
     5041 cagcaggacg gtgaccgcgc cagccacccc ggtcgagatc agcttcggca ccggccgtcg
     5101 gccggaagag tcgacgcacg tccggagcga ttatttcgat ggaccgctgg cgctgctgct
     5161 ggcgttgatc gagcagcgcc agctcgacgt gctgaccgtc cgcctgggcg atcttgccgg
     5221 cgcgtacctc gacgcgctcg cccgcatcga gacaggtcgc ctggggttgc tgagctcgtt
     5281 cgtgacggtg tgctcgcagc tcatcctcat caagacgcgg gcgctgctgc cgcgcgcacc
     5341 gcatctgggc gcggacggca cggcggctga tgcggctgat ccggaggagg agctgcgcag
     5401 gcggctgatc gagtaccgca tgttccgcga cgccggccgc gtcctgggtg atcgccgcgc
     5461 aaccggggcg tggctgtatc accgggaagc ggccgccgcc ctcgctgccg ggctgagtgg
     5521 cgcgcggcca gaccccggcc cagcgctagc accgcagctg ctcgccgacg cgctcctcgc
     5581 cagcgtcaag ctcgtgccgc cggcgcccgc tccgccggag gtcatggcgc gcacgatcac
     5641 gctcgaggag cggaccgagg tcatccgtgc ggcgctcaag cgcgcgccgt tgatcgtcct
     5701 gcaggagctg ctgcgcgacg tgacagaccg cgtcgttgtc gcggtcacgt tcctggcgct
     5761 gctcgagctc gcgaaggggc gcgaggtggc aatcgaccag gcagagccat ggggcccgat
     5821 cacggtcaca aggctgcccg ctgagaatca ggcatccgat ggttgacgag ctgaccgatt
     5881 tgaccgaccc ggccgaccag ccccgggggc ctcttgagcc gccgcccggc gagctgagcg
     5941 cggcccagct cgaggcgctg ctattcgtgg ccgagcggcc gctcgcgcgc agggagatcg
     6001 cgagcctcgc gggtgtcgcg gtcgaggatg tcgatgcgct gctcggggat ctcgaggtca
     6061 cgctgcgcga gcgcggtctg cgcctgctgt ccgccggcga gcacgtccag ctggtcaccg
     6121 cgcccgaggc gggccggctc atcgcgcgct acatcggcgc agacggcggc cgcctctcgc
     6181 cggctgcgct ggagacgctg gcgatcgtcg cctaccgcca acccgtgacg cgttccgtca
     6241 tcgagcgcat acgcggtgtc gactccgact acgtgctgcg cagcttgctc caccgccggc
     6301 tggtggtcga gcagggcagg gcagatgctg tgggtcggcc gttcctgtac gggaccggct
     6361 tcgatttcct ggagcgcttc gggctgacgt cgctcgacga gctgccgccg ctcgaaccgg
     6421 aggccgccct gcggctcgat gagtgaccgc gtgcgcgtcc acaaggcgct cgccgatgca
     6481 ggtgtcgcgt cgcgtcgcgc cgccgagcag atggtgattg ccggtcgcgt cgcggtgaac
     6541 ggcgtgacgg cgacggtcgg cttgcaggtc gacccggccg cggaccagat cacggtcgac
     6601 ggcaagctca tctctggcgc cggccagtcg cgcgtctacc tgctgctcgc caagccggcc
     6661 ggcgtgacga gcaccgtcag tgaccgtcac gccgagcaga cggtgatcga cctcgttcct
     6721 gccgcgctgc gccagggcgc cgcgcggctc tacccggttg gacggctcga ccgcgactcg
     6781 gaaggtctgc tcctgctcac caacgacggc gcgtgggcgc agcgcatgct ccacccgcgc
     6841 cacggcgtcg agcgcgaata cgccgtgggt ctgctccagc cgctgaccgc tgagcagcgc
     6901 gaacagctga cggacggcat catcctcgag gaaggggtcg cgcggctcat cgcgctgcgg
     6961 cagtcgacct cggcggagac gcgccgcctg gtggcgctcc tcggcgacca ggtgcgcggc
     7021 ctcgcctggt accaggtgac gctgggccag ggcatgaagc gccaggtgcg gcgcatgttc
     7081 ggcgccgtga acatgcccgt tcatcggctc gtacggatgc gcatcggcac gctgcgcatg
     7141 ggtgacatgg ccctgggcga cacgcgccgc ctttcggccg cagagcgcgg ccagctggag
     7201 ctgctcgtca cggggcacgg ccgcgacctc gtggtgagcg tcgatgggcc gggcggcagc
     7261 ggcaagagca ccgtcggcat gcgcgccgcc gaagtcgttg gctaccgctt ctgcgacacg
     7321 ggcgtcctgt accgcggcct gacctggcta cagatggcgc gcggcgtcga tgtcgacgac
     7381 gcagatgcgt tggtcggcct gatcgcgcac atgaggctcg agcacgacga gcgccagcgc
     7441 tacgtgcgtc ttgttgccga cgaccgggag gtcaccgacg agctccacac agccgaggtc
     7501 gaccgctcag tgagcagcgt gtcgcagcat gcgcaggtac gcgccgcgct gctgcccgtg
     7561 cagcgcgccc tggctgacgg cgggcggctc atcatggccg ggcgcgatat cggcaccgtt
     7621 gtgctgcccg acgccgatct gaagctgtac ctcgacgtgt cggtcgcgga gcgcgcgcgt
     7681 cggcgggccg cggaacgagg tctggccaac gatccgcgcg cgctcgccca gatcgaagcc
     7741 gagctgcgcg agcgcgacga aatcgacagc acgcgcgccg cgagcccgct gcgcatacct
     7801 gacggcgcga tcgtccttga tacgtccgcc aacacgctcg agcagacggt cgatcaggtg
     7861 gtcgcgctga tccgccgcgc ggagaaggac gactcgctcg atgaatgacg ccgtcgcgaa
     7921 aggccccagc aagggcgtgc cgctgctgct gcgcatcgcg ggtgtgctct cgcgcacgct
     7981 gctgcgcctt ggagcgaggg tcacggtcga ggtcgacggc gatatcccgc gcacagggcc
     8041 gctgatcgtt gtctctaacc acatctcgaa cgccgacccg ccactcgttg caggctggct
     8101 cacaccaatg cttggccggc agatgcacat cctggccaag caggcgctct tcatcgggcc
     8161 gttgggctgg atcctcagga agctcggcgc gacgccggtc aaggccgggg gcagcgacat
     8221 cgaggcatat cgagtcgcgc gcgcagtgct ggatcgcggc gacgtgctct gcatcttccc
     8281 ggagggcacg cgcagcgtca ccggcgtcat gcaggagccc aagccgggcg tcgcgatgct
     8341 cgccactcgt accggcacgc ccatcctgcc tgtcggcgtg agcggcagcg actacttcct
     8401 cggccgcggc aagaagctgc cgcgcctgcg cgcacccatc cgcctgcaca ttggcaagcc
     8461 cttcacgctg acgctcgacc cacacctgcc gcatcgccag gcgatggccg cggcatcgga
     8521 cgagctgatg gaccgcatcg ccgccctcgt cgacgaacgc catcgcggcc gttttggtgc
     8581 ggcgaactag tacaaggccg cccctctgcc gggtgcgagc gtccgtatag ttgccaggtc
     8641 agcagaaccc aggggagaga gacaaggaat gactcaagcg ccggatccca acgcgatgcc
     8701 cggctccagc ccgccgcccg atggtccgca gcccggcccg atgggctacc aggcgccacc
     8761 gtccggaccg acgagttaca tgccaccgcc tgtcgcgccg ccaaagaaga agaccaattg
     8821 gctgggcatc gtcgtgctcg tggccatcgt gggtgcaatc gggggcggct tctggctctt
     8881 ccgtgaccga ctgagcagca atgcatcgga gttggttgtc ggcgactgct ttgacaaacc
     8941 tgcggagaca gagacgatca ccgatgtaca gcaccagccc tgcacctccc cgcacgaccg
     9001 cgaggtgatt accgtgctga cgcacccagc cggcccagac gacccatatc cagttgtgtc
     9061 gggcttcgac gactacatcc aggagaactg cgttcctgcc ttcgagacct acacgggccg
     9121 tgacgcagac accgagtccg agttgaacct gggttacttt cagccgaccc tgagcgggtg
     9181 gggtgaaggc gatcggggtc tgagctgctt catctcgcgc agagacggtc aacagttgca
     9241 tggctcgctg cgcaacatcg ggacttcgcc gctgccatag caacatggcc ggcggccttg
     9301 gtggcaggcc tgatcgcggc tgccgatgcc atacttgacc catcatgggt aacgtcgaag
     9361 aggtccggat cgcgcgccgg accggcttct gctacggggt gcgcgaggcg atcgactacg
     9421 cgcgtctcgc cgccgcgcag ggtaagcaga cccacacgct gggtcaggtc gtgcacaacg
     9481 agggcgtcat cgcccagctc caggcgcagg gcatccagac cgtcgacacg ctcgacgatg
     9541 tcgatcgcgg cgccgcggtg gtcatccgtg ctcatggcgt gacgcccgag gtgatgggcc
     9601 gcgcccagga gcgcggtctc gaggtcatcg acggcacgtg cacttgggtt atccaggagc
     9661 agaaggagct gaagtcactc gtcgacgacg gctacacgat cgtgctgctg ggcacgccgc
     9721 gccaccccga ggtcgtcggg ctgctcggct tcgcgccgga tgcgatcgtc gttgatgagg
     9781 aggaggactg ggagcgcatc ccgcgtacca agcgcatggc gctcatcagc cagagcactc
     9841 agccgccgtg gaagttcgag aagctcgccg catacctcgt gagccgctgc cacgagctga
     9901 agatcatcaa caccgtgtgc cccgtcacca ttcggcgcca ggaggacacg atggcgctcg
     9961 cggccgaggt cgacatgatg gtcgtggtcg gcggtcgcaa cagcgccaac accaaggagc
    10021 tgacccggct ctgccagatc gccaacaagc cggtcatcca gatcgagggc gcgccggacc
    10081 tggccgacgg cggggcgttc ggcagcgcgc gtgtggtcgg tgtcacaggt ggcacttcaa
    10141 cgcccatcga ggacctggag gcggtcgctg agcgcgtctt cgccctcgcc ggcacggccg
    10201 accggcagcc gcgggcgaag gagctcgccc acgcggcgct gactgaggtc gccactccgg
    10261 cctatcgcag cacctcgctc gacgagcacg gtcgcgagaa gggtcacgcc ccgatcgccg
    10321 gcgccgcctg acatgccaac ccgccgcggg aggcggacgc ctcagacagt cgaacgggac
    10381 gcgcccactg aaggtctgcc gatagtcgcc atcgtcggcc ggccaaacgt cggtaagagc
    10441 acgctcttca atcgcatcgt gggcgatcgg gtggcgatcg tggaggatcg cgcccggacc
    10501 acgcgcgaca ggctgtacga cgtcggcgag tggaatggcc ggcgcttcgt ggtggtcgac
    10561 acgggcggcc tcgagacgcg gcccggcgat cccatcgagg agcgcgtcca ggagcaggcc
    10621 cgcgtggcca tcgccgaggc tgacacgatc gtcttcatgg tcgatgccga ggcgggcctg
    10681 acgccggccg acgaggaggc aagcagcctg ctgcgcacgg cgaaggcacc ggtcatcgtc
    10741 gccgccaaca agtccgataa cgcggggcga gagatccagg cggccgagtt tcacgcgatg
    10801 ggctggccgg agacgcaccc catctcggcc atccacggcc gcggcgtggc cgacctgctc
    10861 gacgtgatcg tgtggtcgct cccgcccgaa tccgaatcgg aggtcgagcg caagcgcgcg
    10921 gaagacgagg ccagccgcga cgacgcggtg accgagggtg agaccgagga agccgccttt
    10981 gccgttggcg acaggccggt ccggctggcc atcatcggcc ggcccaacgt gggcaagagt
    11041 tcgctgctca acgcgttgct tggtcagccg cgggcgatcg tgagcgacat cccgggcacg
    11101 acgcgtgacg ccatcgacac gacgctcgag tgggccggcc gcacgatgcg cctgattgac
    11161 acggccggtg tccgccgccg cggcaaggtc gcggccggac cggatgccga gcgcttcgcc
    11221 accatgcgcg ccctgaaggc cgtgacgcgc gccgacgtcg cggtcctggt catcgacgcg
    11281 gtggacggca tgacctcgca ggacgaccac gtcgcggggt atgcgctcga tgagggcacg
    11341 ggcctcgtga tcgcgatcaa caagtgggat ctcgtcgaaa aggacgggca cacgttcgac
    11401 gattacgtcg cgcgcttgcg ccgccacgca ccgttcctgg cgaagacgcc catcctgtcg
    11461 atcagcgcca tgaccgggca gcgcgtcgag cgcgtcctgg aggcggcggt cgagatcgcg
    11521 gccgagcgac gccgccgcat tccgacgccc gcactcaaca cctggctgcg cgacgtcacg
    11581 gcgcgccgcc cgccggcgac ggtcaacggc cgccagccgc gctttctgta tgcgacgcag
    11641 gtcggcagca cgccgccgac gttcgtgctg ttcgtgagcg actcaaaggg cgtgcacttc
    11701 tcctacaagc gctatctcga caaccagctc cgtgcggctt tcggtttcga gggcacgccc
    11761 attcggctcg tcatccgcga gcgcgaacgc gacgaggcac gccggccgcg gcgcagcgcc
    11821 gctcgagcga ctcgctccaa gagccgcgcg cgcggctagg ctgggcacga tggaacgcca
    11881 cgcaacgcgc gccgtcgtgg gcgccggcca atggggcacg acgctcgccc ttcacctgtc
    11941 gcggcgcggc ccggtgacgc tcctggcgcg cgacgaggag caggcgcgcc agctcatcgg
    12001 ggagcggcgc aacagccgct acctgcctga catcgcgctg cccgttgaga tcgagatcac
    12061 cgccgacccg gccgcgctcg cgagcgccag cgacctggtc atcttcgcgg tgccgtcgcg
    12121 ggccatgcgc acgtccgcgg agcgcgtccg cggcaacctg cacaacgagg cggtcctcct
    12181 gtccgtggcc aagggcctgg agcagggctc gctcgatcgc atgacgcagg tcctggccga
    12241 cgtcctgccc gaggccaagg agaggatcgg tgcgatgtcc ggacccaacc tcgcgctgga
    12301 gatcgcgcgc gggttgcccg cgagcgccgt gatcgccgcg gaggacgtgg aggtcagccg
    12361 gacggcgagc gagatcatcg gcacgcgctc gttccgcgtc taccgcaacc gcgacgtgac
    12421 cggcgtcgag ctatgtggcg cgctcaagaa cgtaatcgcg atcgccgccg gcgcggccga
    12481 gcagctgggt ttcggcgaca atggcaaggc cggcctcatc acccgcggcc tggcggaaat
    12541 gacgcgcctc ggcatggccg ccggcgcaca cccattgacc ttcgccgggc tggccggact
    12601 gggcgatgtc atcgccacct gctactcagc cctgtcgcgc aaccacgcgc tgggcgtgga
    12661 gctcgccaag ggccgcaagt ggtccgacat cgagggcacg ctcgcgggcg tggcggaggg
    12721 cgcgtacacc gttgaggccg cgctggagct ggccaagcgg cacaacgtcg agatgccgat
    12781 cgcccaggag gtgcacaacg gcatctacga gggcaagagc gtccaacgct gcctgatcga
    12841 cctgctgtcg cgcgagcaga aggacgagct tgcggacgtg gagcaatggg cgcgtcgcgc
    12901 atatggcacc aagttgtcca caggcgtgtg agaactctgc aacaaccctt ctactgtctc
    12961 tggccaaact agaccgcacc tatccgcacc cccacccggt acccgagtcc gggcccttgg
    13021 caaatggtcc gtatggagcc gtcggctcgg cacgactaac gtgcgcggca agaacccgcg
    13081 ttggggaagg caatcggata ggccaagtga ggaccgtcga agcggagccg tgagtcgcgc
    13141 gccagcgcta cttgtccgcc tcagataaca gtcggtctca gcttcgctcc gttgggtcga
    13201 gcgctcgcgg tctggctgac ctacaccgag ccgccggcgt cgaagcaagg tctaccgatt
    13261 gcccccaact ccctgtgttc gggggccgct acctctccgc tagacttgcc gtgtcggggc
    13321 gtagcgcagc ctggtagcgc acttgaatgg ggttcaagag gtcgaaggtt cgaatccttt
    13381 cgccccgacc acttgttcca gcacgaaaga gccccgcgac gaaacgcgat caggcgggcg
    13441 cgtcgtgcgc ggcatggccc gcgagccggc ggccgatggc ggcaagcgcc tcgccgacag
    13501 cgctgtattg ctcaggcggt atcgccagca cctgcgggtg gttatcggga tgctctggga
    13561 cgattacgag cgtcgcggga cgcaggcgct caatatcgaa ctggattctg cgcacgccct
    13621 cgaatgggat atccaggtcc ggccgatccg tgcggctcac taccgccagg cgccggtcgg
    13681 ttacgacaag ccggctgcct tcgccctggg cctcgacctg aatcttctcg tcggcgtgaa
    13741 gcagttgtgt aagcaactcg tcgctcactg aaatatgtcc tcgctagaaa tgccaagcca
    13801 ggcgaatcta ggcgattccg gcccgcgttg acgtggcgat cagtggttgt gccgttgcat
    13861 caaccgtcca acatggtgcc gcgaagtgcc ggctgcagcc gcgccagtgt cagtcgctgg
    13921 gcgtgatccg aacggtcgcc gtcatgttcc agaacagctg ctcgtcgtgc ctgtccggcc
    13981 tgatggccac cgcgaaaacc acattgccgg caggccccgt gccgcggacc tgaatctgat
    14041 ccacatggcc ggcgaggacg aggtccggca gcgcttcgaa ggtcactgtc gcggcatcgc
    14101 caacgtgcac gtgcacgacc tcgagctcgc tcaggtcggt cgtctcgatc agccagcccg
    14161 ataggtcgcc gatcgtaacc accggctcac ccgcgtgggc ctgctcgcca cgttcgacat
    14221 tgatggccgc gaccgtgccg ctgaacggtg ctcgcagctc agtctggcgc agcgcgacct
    14281 gggcttcgct cagcgccgac gccgccacat cacggtcggt tcgtgccagg cgcagttcaa
    14341 cctgcgcctg ctcgatctcc gccggcgtcg cgtcctcggg cagccgctcg acctgcagct
    14401 ccgccttcgt cacggcagcc tcggcactgt ctagctcctc ctgggccacg tcgatcgccg
    14461 cctcgtacgt cgactggtcg agcctgagga gcagctggcc gatcgtcacg gcgtcgagct
    14521 cgtgaacgaa gatctggtcg acgatgccgc tcaccggcag cgccaggtcg gcgctgcgct
    14581 ggggcaccac gacagctgtg gcgcggatgg ccgtgtcgat aggcgttggc gagggccgca
    14641 cggaagccga cgggcggggg ctgaggctgg gccggaccga cggcgaaggc gtggcccgcg
    14701 ggccaatgaa ggcgccaccc agcaaaaggg cgacgatggc ggtcaccacc acgccgatac
    14761 ccatgccgta cagcagtgag cgattcatct ggcgcgccct agactaaccg ggcagaaaca
    14821 ggaggaaaga catggcagca gttcgttcag cgcgtgcgca gtggcagggc aatctgaccg
    14881 agggcagcgg atcggtcagc tcgctgtcga gcggccagct ggtcgatctg ccgatcagct
    14941 ggtcagcacg aacggaagac ccggccggtc tgtccggtcc ggaggagctg ctggcagccg
    15001 cccatgcatc gtgcttctcg atggcgttct cgaacacgct cttcaaggct ggcttcacgg
    15061 cggagcacat cgagacaacg gtcgagatca ccggcgacaa gcgcgaggcc ggctggaccg
    15121 tcatctcgtc gcacatcacg gtgcgctgcc gtgtcccggg cgcgagcgac gccgacatcg
    15181 cggatgctgc cgagaaggcc aaggatggtt gtccaatctc gcgcgccctc aagggcaacg
    15241 tcgaactgtc cgtcgacgcc acgctcgagt agtagggcca gcgcacatgc cgccgatcga
    15301 ccacctgcag cgggtgccga tctatcacct cgaggccctc gagcgccagg gcatcttcac
    15361 caccgggctg ctgctcgagg tgtctgagac gcccacccgg cgccagtacc tcgccgacca
    15421 gatcagcgcc acggtcaacg atgtccacga atggcgcgat gaggcgctgc tgctcaacct
    15481 ggccaacttc gggccggccg agcaacggct attcacccag gccggcatcc ttggcctctc
    15541 cgacctgctg gcgctcaatg ccgcggaata cgagcagcgg cttgcgcgct ccgcccgggc
    15601 gctcggcgtt gagctgccgc ccagtctcgt ggtcgaagga tggatggagc aggcgcacac
    15661 cctggcggag gagtagcggt agcgcggccc gcccgcgact ggttgagctg gctgctgccg
    15721 ccagccacgg cgctggtcat catcgtggtc ggcgttctgc tgctgacgac gtctatctgg
    15781 atgcatttcg cgatcccagc agcgggcggc ggtctccccg gttatccgat tgaggcctcg
    15841 ctggttgcgt ccgaccgcac cgtccgccag ctcctcggac tgggcgactt cctgatctcg
    15901 gttgagtgtc ctggggccgc accatgtcca ccgcaagaat tgctgtacac cgccgacgag
    15961 caggcgcacc tgcgggatgc tcgcgcggtg ctctacctgt tcgtcgcgct cgcggtcgca
    16021 tcgctcgcgt ttgtcatagc agcactcgct catcgccccc acgacgcgcg gcgctggcga
    16081 gcggttgcgc gcggcggggc agggctggcg atcggggctg tcgtagtcgg ggtcgtcggc
    16141 tacctcgtgt tcgacgcggc gttcgagctg tttcaccgcg tcctattccc cggcggcaac
    16201 tgggagttcc cggcggacag caacatgatc cggctctacc cgtatgcgtt ctggcagctg
    16261 accgcggccg cgctcggcat gctgtccgtg atcggcggcg gtctggtctg gtggctcgcc
    16321 cgtcgccgcg ctgcgagtct tgccgaatga gccacgaact gccaccgctc atgtcgctcg
    16381 aggacgcgcg ggcgcgcatg ctcgaggggc tcgcggcgct ccccacggaa atggtcgcgc
    16441 tcggtgatgc cgcgggccgg gtcctggccg tggatctcac cagccgccac acgctgccgc
    16501 cgtgggacaa cagcgcgatg gacggcttcg cggtgatcgc cgcggacgtc gccatggcct
    16561 cgaaggacgc gccggtgcgg ctcgcggtca cgggcgaagc ggcggccggg cgggtggccg
    16621 atgtggctgt cacaagtggc aaggccctgc gcatcctcac cggcgcaccg gtgccggcgg
    16681 gcgccgacgc ggtcgtgccg gtcgagaaca cggacgcgcg gccgggcgtc gccgatctgc
    16741 cgcgcgaggt cgccatctac tcagcggcgg agcccggcgc gcacatccgt cgcgcgggca
    16801 gcgacctcac tgcgggcgcg ccactgcttg aggcagggac ggagctaacg cccgccgcgg
    16861 tcgccgtcgc cgcggcgggt ggacacgcgc agctcacggt tcaccgcccg ccgcacgtgg
    16921 ccgtcctcgc gactggcgat gagctggtgg cacctggtca gccgcttggc cccgcgcaga
    16981 tccctgacag caacaccttc ggcgtggccg cgcaggcacg cgagctgggc gctgacgtgc
    17041 aggtcctggg catcgcgcgc gacgacctgg acgacgtgct cgcccatctg cgccaggggc
    17101 tggggtgggc cgacgtgatc gtggccagtg gcggcgtatc ggtcggtgcc cacgacgtcg
    17161 tcaaggacgc gttcgcgcag atcggccgga tcgacctgtg gcgcatcgca gtgcaacccg
    17221 gaaggcctct ggcgttcggc cgcgcccccg ccacggacgg tcaacgcgag gtgcttctct
    17281 ttggcctgcc gggcaacccg gtcagcagcc tcgtcacctt tgagttgttc gtgcggccgg
    17341 tgttgcgccg cctcgcgggc cagcacgacg tgatcgggcg cgacatcgtc cgcgcggcgc
    17401 tcgagcaacc cgtgagcaag gcgcacaacc gccgcgcctt catccgcgtg aagctcacgg
    17461 cggctgatga cggtctcggc tggcgggcca gtctggctgg tggccaggat tcgcatgtcc
    17521 tgtcggccct cgcagctgct gacggattgg cgatcattgg cgaagaattc gatagcgtcc
    17581 ccgctggcac acaggtcgag gtgatcagga tccgatgagg cagccaccag accgctccga
    17641 gcgtcgccgc ctgacccacg tcagccgtgg tggccggccg cgcatggtcg acgtgagcgc
    17701 aaagcccgcc accgcgcgcc gagccgtcgc ggaagcgttc gtgacgctcg agcaggagac
    17761 gctgaccgcg atcatcgacg gccagaccac caagggcgac gtgctgacgg tggcggagat
    17821 ggccggcgtc atgggcgcga agaagacgtc cgagctcatc ccgctgtgcc atccgatcgc
    17881 gctgaccgac atcgtggtcg agatcacccc ggagcgcgcc ggtcccggcg tgcgtatccg
    17941 cgcgacggcg gcgaccgtcg ggccgacggg cgtcgagatg gaggcgctga cagccgcgtc
    18001 ggtcgccgcg ctcaccgtct acgacatggt caagggcgtc gatcgcgggg ccgcgatccg
    18061 cgaggtgcgg ttgctcgaga agtcgggcgg caagtcgggt gagtggcggc gcgcccccgg
    18121 tgacaccgcg ccacgccctg gtgtcccgac aaaacgcggc tcgttccgca agcgtcccga
    18181 acgttgagca aggttgcgct ggtactgacg atcagcgacg gagtcagcgc gggcacgcgc
    18241 caggatgaaa gcggcgcggc tctcgcggcg cgcctgaccg agctcggcta cggcgtcgag
    18301 cgcgcgaccg tggccgacga gatggatgag atcgcgactg cggtcagcgg ggcgacaggc
    18361 cacgcagcgc tcgtcatcag caccggcggc accgggcttg gcccgcgcga cgtcacgccg
    18421 cagaccctgc acggactgct cgactacgag atcgcgggtt tcggcgagca gatgcgcgcg
    18481 ttcggccgca cgaggacgcc catggccgac ctgtcacgca gcgtggccgg cgtgctcggc
    18541 acaaccctgg tcatcgcggt cccggggagc cgcggcggcg ccctcgactc gctgaccgcg
    18601 atcgagccgc tgctcgacca cgccctcgag acactcggcg gtcacaccca gcacgcggcc
    18661 agcggcgaag gcaagagcta gatggtcgag cgcgtcccgc tctacccgct cgcgcccacg
    18721 ctcttcatcc tcgcggtcat cggcttcgta ctactgatgg cgcgccacat gcgcgtcttc
    18781 gcgctcgcac ggcccgcgcc ggtcggcgat caggcgggct ggttcgcgtc gatgttcaag
    18841 tacgcgatcg tccagatacg gatgttccgc gacctcgacg cgggcctcat gcacgcgacg
    18901 atcttctggg gcttcgtgat cctgacggtc ggcacggcgg accgcgtcac cttcggcctg
    18961 atcgagcgca tcgtccggcc gctcctcgat ggctggctat ggcggctgct gatgctgggc
    19021 cagaacatgt tcgtgctcgg tgtcctgatc atggtcggct acgcgctctt ccgtcgactc
    19081 gtgtggcgct acccgagaag gacgacgctc tcacgcgacg cgctggtcat cctgttcttg
    19141 atcggtggcg tggtcctctc ggaatggttc gccgagggtt tccggatcgc cgcgtacggc
    19201 gatcccgacg cgggctgggc ggtcatggcc acgccgctgt cctgggtcat caatggattg
    19261 ctgccggcgc aggtgctcgg cgcctggtac gcgctcttct tctggatgaa cgtcgctctc
    19321 gtgtcgttct tcctcatcta cctgccgcac tccaagcacc tccacatcgt caccgcgttc
    19381 ttcaatgccg cgctgcgcaa gaccaggccg cgcggcgagc tgccgcagat cgatctggag
    19441 gcagcggacg cccgattcgg cgtgaagacg gtggccgacc tgtcgtggaa ggacctgctg
    19501 gacgggttca cctgcaccga gtgcgggcgc tgccaggagg cgtgcccggc gtgggcaact
    19561 ggtaaaccgc tcaaccccaa gacgatgatc atgggcatcc gcgagatgtc ggttgaggcc
    19621 gaggcgggcg tgccgctcat cccgtggatc cggcccggcg gcggcaccgc gatccacggc
    19681 actgacaagc cgatcgtcga cgcagcgatt ccctacgacg cggtgtggga ctgcgtcacc
    19741 tgcggcgcgt gcgtcgaggc ctgcccggtg atgatcgagc acgtcgacaa gatcgtcggg
    19801 ttgcgccgct cgctcgtcct cgaggactca cgcttccccg aggaattgac ggcgtcgttc
    19861 aacaacctcg agcgctactc caacatctgg ggccagccgc aatcgacgcg gctcgactgg
//
LOCUS       DATBRX010000103         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1372366, whole genome shotgun sequence.
ACCESSION   DATBRX010000103 DATBRX010000000
VERSION     DATBRX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372366"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VIF08_03820"
     CDS             <1..1099
                     /locus_tag="VIF08_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY8135143.1"
                     /translation="QRPVQSAVIYGGVGFDNQVRQIRQGPEIVVATPGRLLDHVGQRT
                     IDLSRVEVLVLDEADRMLDMGFIHDIKKVIALLPVARQNLLFSATFSAEIRRLAAEFL
                     KDPAYVQVTPKNTAPELVKQLVIPVDKSRKRELLAQLVKSGRIYQALVFTRTKHGADR
                     LVQQLSLDGISAVAIHGNKSQPQRVRALNDFKRNKVAILVATDIAARGLDIDALPHVV
                     NYELPMVASDYVHRIGRTGRAGTDGDAISLVCVDENAMLREIETLLGHKIEQEIIPGF
                     TPDRSIRPQAIQTRSPRPFQPRHQPEARQRGFGRPGQAAGRPAYGQRRPANGSSRPSY
                     GQSRPAHGGHRPPQASIGRPMALPGERLSRG"
     gene            complement(1111..>2747)
                     /locus_tag="VIF08_03825"
     CDS             complement(1111..>2747)
                     /locus_tag="VIF08_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8135144.1"
                     /translation="FAERAEALRQEAMLTERLSATVSALEHASEAKSDFLASMSHELR
                     TPLSAIIGFSALMRDQPLEDGKRPIPDEWITHVRRSGDHLLSLINDVLDLTKIEAGRI
                     DLAPEAFDLATALAESVEGLRPLADKKHIDLKLEAAPGVIEADRGRLRQIVYNLLSNA
                     IKFTPDEGRVVVQARWDRADAVIAVTDTGKGIAPEDVGHIFEEFRQVGDLKDRAAGTG
                     LGLALSRRLAEAHHGSITVVSALGSGSTFELRLPRGTVVELPVEPARPETARSAGAAL
                     VLVIEDDPAAVRLLRTYLEDAGHLVEATSDGESGMAAARRLNPDAIILDVLLPGIDGW
                     EVMRRLKADADLRDIPVVVLTVVDERNVAMSLGAADYFLKPVHRDALLARLASYTFTT
                     KVKLHTVRVLVIDDDPAARELVRQALEPEGFEIIAADSGPAGVALARQMRPDLVICDL
                     VMPGMDGFEVVQALAESDLPAQVPIMILTSQQLSDTDRERLNGRVAGIVQKSEDLRVS
                     LDRWFSRASRLARRRREIDAMPAVQAPPVELAAPRP"
BASE COUNT          457 a          960 c          904 g          426 t
ORIGIN      
        1 gcagcggccg gtccagtcgg ccgtcatcta cggcggtgtc ggtttcgaca accaggtccg
       61 gcaaattcgc cagggtccgg agatcgtcgt cgccacgccg ggccggctgc tcgaccacgt
      121 gggccagcgc accattgacc tgagcagggt cgaggtgctg gtgctcgacg aggccgaccg
      181 gatgctcgac atgggcttca tccacgacat caagaaggtc atcgcgctgc tccccgtggc
      241 gcgccagaac ctgctgttca gcgcgacctt cagcgccgag atccgccgcc tcgccgccga
      301 attcctgaag gacccggcgt acgtccaggt cacgcccaag aacaccgcgc ccgagctcgt
      361 gaagcagctc gtcataccgg tcgacaagag ccgcaagcgc gagctcctgg cgcagctcgt
      421 caagagcggc cgcatctacc aggcgctggt cttcacgcgc accaagcacg gcgccgatcg
      481 gctcgtccag cagctgagcc tcgacggcat cagcgcggtc gccatccacg gcaacaagag
      541 ccagccccag cgcgtgcgcg cgctgaacga cttcaagcgc aacaaggtgg cgatcctcgt
      601 cgccacggac atcgcggcgc gcggcctgga catcgatgcc ctcccccacg tcgtcaacta
      661 cgagctgccc atggtggcgt ccgactacgt gcaccgcatc gggcgcaccg gccgtgcggg
      721 caccgacggc gacgcgatct cgctggtgtg tgtcgacgag aacgccatgc tccgtgagat
      781 cgagaccctg ctgggacaca agatcgagca ggagatcatc cccggcttca cccccgaccg
      841 atcgatccga ccgcaggcca tccagacccg atcgccccgt ccattccagc cacggcacca
      901 gccagaagcc aggcagcgcg gttttggtcg gccgggccag gcggctggtc ggcccgccta
      961 cggtcagcgt cgacccgcca atggttcgag tcggccttca tacggtcaga gtcggcccgc
     1021 ccacggtggt catcggccgc cccaggcctc aatcggccgc ccaatggccc ttccgggaga
     1081 gcgcctctcc cgcggctagc gcgcgaaagg tcagggacga ggagcggcga gctctactgg
     1141 cggtgcctgg acagccggca tggcatcgat ctcgcgccga cgccgcgcca ggcgcgatgc
     1201 gcggctgaac cagcggtcga ggctcaccct gagatcctcg gacttctgca cgatgcccgc
     1261 gacgcgtccg ttgagtcgct cgcggtcggt gtcggatagc tgttgcgacg tcaggatcat
     1321 gatcggcacc tgggccggca ggtcggactc ggccagcgcc tggacgactt cgaagccatc
     1381 catgccaggc atcaccaggt cacagatgac gaggtccggc cgcatctgcc gggcgagtgc
     1441 tacgcctgcc ggcccggagt cggcggcgat gatctcgaag ccctctggct cgagcgcctg
     1501 cctgaccagc tcgcgcgccg ccggatcgtc atcgatcacg agcacacgca ccgtgtgcag
     1561 cttgaccttc gtggtgaatg tatagctcgc cagccgggcg agcagtgcgt cgcggtggac
     1621 cggcttgagg aagtagtcgg ccgcgccgag gctcatcgcg acgttgcgct catcaacgac
     1681 ggtcaagaca acgaccggga tgtcgcgcag gtccgcgtca gccttgagcc ggcgcatgac
     1741 ctcccagccg tcgatgcccg gcagcagcac gtcaaggatg atcgcgtccg ggttcaggcg
     1801 acgcgcggcc gccatgcccg attcgccgtc cgacgtggct tcgaccaagt gcccggcatc
     1861 ctcgaggtac gtgcgcagca ggcggacggc ggctggatcg tcctctatga caaggacaag
     1921 ggctgcaccg gcagaccggg cggtctcagg acgcgccggt tcaacgggta gctccacgac
     1981 ggtgcctcgc gggagtcgca actcgaaggt cgacccggaa cccagggccg agaccacagt
     2041 gatgctgccg tggtgcgcct ccgccaggcg gcgtgacagg gccaggccca ggccggtgcc
     2101 ggcggcgcgg tccttgaggt cgcccacctg gcggaactcc tcgaatatgt ggcccacgtc
     2161 ctcaggcgcg atgcccttgc cagtatccgt gacggcgatc accgcgtccg cgcggtccca
     2221 gcgcgcctgg acgactaccc tgccctcgtc gggcgtgaac ttgatcgcgt tggacaagag
     2281 gttgtagacg atctggcgca gcctgcccct gtccgcctcg atgacgccag gtgcagcctc
     2341 gagcttcagg tcgatgtgct ttttgtcggc aagcgggcgt aatccctcga ccgactctgc
     2401 caatgccgtg gcgaggtcga acgcctcagg cgccagatcg atccgacccg cctcgatctt
     2461 ggtcaggtcg aggacatcgt tgatcagcga cagcaggtgg tcgccgctgc gccgaacatg
     2521 ggtaatccac tcatcgggaa tgggccgctt gccgtcctcc agcggctggt cgcgcatcag
     2581 cgccgagaag ccgataatcg cgctcaaggg ggtgcgcagc tcgtggctca tgctcgcgag
     2641 gaagtcgctc ttggcctcgc tggcgtgctc gagggcgctg acagttgcgc tgagccgctc
     2701 ggtcagcatc gcctcctggc gcagggcctc tgcccgctcg gcgaaga
//
LOCUS       DATBRX010000104         5425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3048817, whole genome shotgun sequence.
ACCESSION   DATBRX010000104 DATBRX010000000
VERSION     DATBRX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048817"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..800)
                     /locus_tag="VIF08_03830"
     CDS             complement(156..800)
                     /locus_tag="VIF08_03830"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8135145.1"
                     /translation="MDGHPWALYDLRIWTERLELRLPTEDELYQLLDLARSGIHDPGD
                     MPFGFAWTDQPSPQLERSFMQYHWSTRASWSPGKWSLDLGVWSEGQLLGTQGIDAENF
                     GVLRQVSTGSWLARQFQGRGVGKEMRQAVLGFAFDHLGAVWAKSGSFRENPASAAVSR
                     AVGYEEDGLEILAPRGEPRELVRWRMHVDQWRAQERPRIEVGGLERCWDMFGAG"
     gene            952..2265
                     /locus_tag="VIF08_03835"
     CDS             952..2265
                     /locus_tag="VIF08_03835"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8135146.1"
                     /translation="MGPIGRLKQVRMLQPLRERDFALLTGGSAVSLIGDGFFHVALAW
                     QVYQISNVPTAMSFVGVAATLPLVIFILIGGTFSDRYDRRRLMIGADLLRGLAIGAMG
                     VLSMAGVLELWHVAAMMAVVGLGDAFFNPSATAIVPDLLTEEQLPQANAISGVLRRFM
                     LSIVGPALAGFTIAIFGPGQAFVIDALTFLVSAAAVYAIRARPQLRPKADLGVRQTVH
                     DIGEGMGYVRARPWIWATLLSAMFSLLVFIGPVQVLVPFLVKNRLGLGPEALGAIFAV
                     GGVGSILMAVAIGQLGLPRRRVTVMYVAWTLGIGVMAIYGVMDSLWQALLVAAFSHSM
                     FELGQVIWTTMLQQLVPRNLLGRVSSVDWLVSIGLVPLSYALTGPASDLLGPGTTMVY
                     GALAGAVITILLILVPGVRDPEKVTPPPPKETGLVVSTSPEQDAL"
     gene            complement(2294..2980)
                     /locus_tag="VIF08_03840"
     CDS             complement(2294..2980)
                     /locus_tag="VIF08_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135147.1"
                     /translation="MRATFGLIGSIALAMVMIGCGGAPAGPTLAPGQTATPPGATQPP
                     GGQTQPPAGVTGHECDAIPTMDITNPNPATPVPDTTLTGHFPATIDGQPVTDVESMQW
                     LYLMCQFGGQAAVNSAASQSGGLNITTMSFGSATANVDGEDVDISAFRTPGQDANALV
                     QTLATIAAQSGTDINPGNVSSANIGGKNVFVWTDTDGSKGYAYPAGDTLIMFDSVTDS
                     QATKILAALL"
     gene            3059..3544
                     /locus_tag="VIF08_03845"
     CDS             3059..3544
                     /locus_tag="VIF08_03845"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY8135148.1"
                     /translation="MRDSVSARLGRPFDPAELRAIKRLWVRHSIAEDRRDIDGLVATI
                     APHGEYEMVPTRQRWYGHTGVRGFYAELFAAFPDNRFELAEVVVGPQGVFEAARLTAT
                     NLGPWAGIPASGLPVDLEVLILFPWDPQARLFLGEKIWFDASGLNLVPGRSGGATRDQ
                     G"
     gene            complement(3515..4765)
                     /locus_tag="VIF08_03850"
     CDS             complement(3515..4765)
                     /locus_tag="VIF08_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8135149.1"
                     /translation="MTAPPVTTQGLKRHWLLDPDVTYLNHGAFGSCPGAVLRVQDEWR
                     TRMERHPVQFHDVELEGHLARARERLGELLNAQASDLAFVPNATTGVNTVLASLEFAP
                     GDEILSTDHEYNACLNAARRVAKRHGATVVVVPLPFPTMSPDEMADALLERVTQRTRL
                     VLISHVTSPTGIRLPVERLVPELAQRGIDTLVDGAHAPGMLALDLDALGAAYYTGNCH
                     KWLCAPKGAGFLHVRRDRQALIHPLVTSHGANSPRADESPFRLEFDWTGTSDPTAYLS
                     IPAALDFLDGLLPGGWPDVMRHNHELVVAGRQTLLNAIGGEPLAPDTMLGSLAAIELP
                     EDLRPSPTPPAADADPHATYPADPLRDALSDQDHIEVPVFTWPHTPADTAPRRRLLRI
                     SAQLYNEPADYERLASALVARRPA"
     gene            4800..>5425
                     /locus_tag="VIF08_03855"
     CDS             4800..>5425
                     /locus_tag="VIF08_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY8135150.1"
                     /translation="MCGRMTMRTDVSDVALIFDAEVRDPGAFADLGPRYNVAPTQPIE
                     VVVQRDDGRFIELHRWGLVPSYAKSVSAGNRLINARAETIATTPAFRASFARRRCIIP
                     ADGFYEWRREPGGKRKQPFLIHAADDRPLAFAGVWSPWKDPATGEWVLSAAVVTTSAN
                     AVVGQLHDRMPVILEPLAWPIWADPEIRDPGLLTDLLQPAADGLLMLTA"
BASE COUNT          763 a         1829 c         1902 g          931 t
ORIGIN      
        1 tgggcgccgg gctcgtcatc tggattttgg tccagctggt ggtgatgcct gaggtgatgt
       61 ggctgcagtg ggccttcctg gcggtcggcc tggtgctcgg ctcctgcgcg ctcctctggc
      121 tgcgccggac cggccagctc aagctctggt agggcctagc cggcgccgaa catgtcccag
      181 cagcgctcga ggccgccgac ctcgatgcgc ggccgctcct gcgcgcgcca ctgatcgacg
      241 tgcatccgcc agcggacgag ctcgcgcggc tcaccgcgcg gggcgaggat ctcgagtccg
      301 tcctcttcgt atccgaccgc gcgcgacacc gccgccgacg ccgggttctc gcggaacgac
      361 cctgacttgg cccacaccgc gcccaggtgg tcgaacgcga agccgagcac ggcctggcgc
      421 atctccttac cgacgccgcg gccctggaac tggcgcgcca gccacgaccc cgtcgagacc
      481 tggcgcagca cgccgaagtt ctcggcgtcg atgccctgcg tgccgagcag ctggccttcc
      541 gaccacacgc ccaggtcgag cgaccacttg ccaggcgacc acgacgcacg cgtgctccag
      601 tggtactgca tgaacgagcg ctccaactgc gggctgggct gatcggtcca ggcgaagccg
      661 aagggcatgt cgccggggtc gtggatgccg ctccgtgcca ggtccagcag ctggtacagc
      721 tcgtcctccg tgggcaggcg cagctcgagg cgctcggtcc agatgcgcag gtcgtacagc
      781 gcccacgggt gtccgtccat cagggagcga gtgtagtcat ccacatgaca gccccggccg
      841 gctgaccttg ttatctccgc aaatcgcccg ccggcgtttg ctgcgcgctg tggtgcgacc
      901 cgcgaccttg gccgcgtgtc gcccttccgc ttgcgggcta gagtcgggtt ggtgggtccg
      961 atcggccgtc tgaaacaggt gcgcatgctc cagccgctgc gcgagcgcga cttcgcgctg
     1021 ctcaccggcg gcagcgcggt gtcgctcatc ggcgacggct tcttccacgt cgccctggcg
     1081 tggcaggtct accagatcag caacgtgccg acggcgatgt cgttcgtggg cgtggccgcc
     1141 accctgccgc tggtcatctt catcctcatc ggcggaacgt tctccgaccg ctacgaccgc
     1201 cgccggctga tgatcggggc cgacttgctg cgcgggctgg ccatcggcgc gatgggtgtg
     1261 ctatcgatgg ccggggtgct cgagctgtgg cacgtggccg cgatgatggc ggtcgtcggc
     1321 ctgggcgacg cgttcttcaa tccgtccgcg accgcgatcg tgcccgacct gctgaccgag
     1381 gagcagctgc ctcaggcgaa cgccatcagc ggggtcctgc gccggttcat gctgagcatc
     1441 gtcggcccgg cgctggccgg cttcacgatt gcgatctttg ggccgggtca ggcgttcgtc
     1501 atcgacgcgc tgaccttcct tgtctcagcc gccgcggttt atgcgatccg agcccggccc
     1561 cagcttcgtc cgaaggcgga cctgggcgtt cgccagacgg tgcacgacat cggcgagggc
     1621 atgggctacg tccgcgcccg accatggatc tgggcgacgc tgctatcggc gatgttcagc
     1681 ctgctggtct tcatcggacc ggtccaggtg ttagtgccct tcctggtcaa gaaccgcctt
     1741 ggcctgggtc cggaggcgct gggcgcgatc tttgccgtgg gcggcgtggg ctcgatcctg
     1801 atggccgtgg ccatcggcca gctgggcctg ccgcgacggc gcgtcacggt catgtacgtc
     1861 gcctggacgc tgggcatcgg cgtgatggcg atctacggcg tgatggactc actgtggcag
     1921 gcattgctgg tggccgcgtt ctcgcattcg atgttcgagc tgggccaggt gatctggacg
     1981 acgatgctcc agcagctcgt gccgcgcaac ctgctgggcc gcgtttcgtc cgttgactgg
     2041 ctcgtgtcga ttggcctggt gccgctgtca tacgcgctga ccgggccggc gtcggacctg
     2101 ctgggcccgg gaacgacgat ggtctacggc gcgctcgcgg gcgcggtcat cacgatcctg
     2161 ctgatcctgg tcccgggcgt gcgcgatccg gagaaggtca cgccgccgcc accaaaggaa
     2221 acggggctgg tcgtttcgac cagccccgaa caggacgccc tgtgacagct gcctggcgca
     2281 gcttggatct gccttacagc agagcggcga ggatcttggt cgcctgcgaa tcggtgacgc
     2341 tgtcgaacat gatcagcgtg tcgccggcgg gataggcata gcccttgctg ccgtcggtgt
     2401 ctgtccagac gaagacgttc ttgccaccga tgttcgcgga gctgacattc cccggattga
     2461 tgtccgtgcc gctctgggcg gcaatggtcg ccagggtctg gaccagcgcg ttggcatcct
     2521 gaccaggcgt gcggaacgca cttatgtcca cgtcctcacc gtcgacgttc gccgtcgccg
     2581 agccgaagct catcgtggtg atgttcagcc cgccggactg cgacgcggcc gagttgaccg
     2641 ccgcttgacc gccgaactgg cacatcaggt acagccattg catcgactcc acgtcggtca
     2701 cgggctggcc atcgatcgtg gccgggaaat ggcccgtgag cgtcgtgtcg ggtaccggtg
     2761 tagccgggtt ggggttggtg atgtccatcg tcgggatggc gtcacactca tgcccggtca
     2821 cgccagccgg cggctgagtt tgtccgcctg gcggctgagt cgcgcccggc ggtgtggcgg
     2881 tctggcccgg ggcgagcgtc ggcccggcgg gcgcgccgcc gcagccaatc atcaccatcg
     2941 cgagggcgat ggaaccgatc aacccaaatg ttgcacgcac tcttatgtct cctaacggct
     3001 tcacagcgca gtcatcgtac ccggcccgag cggcgccggt tataaagaaa gtcatcaaat
     3061 gcgcgacagc gtcagtgccc gcctcggccg ccctttcgac ccggcggagc tgcgcgccat
     3121 caagcggctg tgggtgcgcc actcgatagc ggaggaccgg cgcgacatag atggcctcgt
     3181 cgccaccatc gcgccgcacg gcgaatacga gatggtcccc acgcgccagc gctggtacgg
     3241 ccacaccggc gtacgaggct tttacgccga gctgttcgcg gcctttccgg ataaccggtt
     3301 cgagctggcc gaggtcgtcg tcgggccaca gggcgtcttt gaagccgccc gactcaccgc
     3361 cacgaacctg gggccatggg cagggatacc tgcgtcgggc ctgccggtcg atcttgaggt
     3421 gctgatcctg ttcccgtggg acccgcaggc gcgtctcttc ctgggcgaga agatctggtt
     3481 cgacgcgtcc gggctcaacc tggtgcccgg ccgctcaggc ggggcgacgc gcgaccaggg
     3541 ctgacgcgag gcgctcgtag tcggccggct cgttgtagag ctgggcgctg atgcgcagca
     3601 gccggcgccg tggcgccgta tcggccggcg tgtgcggcca ggtgaagacc ggcacctcga
     3661 tgtggtcctg gtcgctgagc gcgtcgcgca gaggatctgc cgggtaggtc gcgtgcgggt
     3721 cggcatcggc ggcaggtgga gtgggtgagg gtcggaggtc ttcgggaagc tcgattgccg
     3781 ccagcgaccc gagcatcgtg tcgggcgcga gcggctcgcc gccgatggcg ttgagcagcg
     3841 tctggcgacc ggcgacgacc agctcgtggt tgtggcgcat cacgtccggc cagccgcccg
     3901 gcagcagccc gtcgaggaag tcgagcgcgg ccgggatcga cagatatgcg gtcggatcgg
     3961 aggtgcccgt ccagtcgaac tccaggcgga agggcgactc gtcggcgcgc ggcgagttcg
     4021 cgccgtgcga cgtcaccagt gggtggatga gcgcctggcg gtcgcgccgc acgtgcagga
     4081 agcccgcgcc cttgggcgcg cacagccact tgtggcagtt gccggtgtaa tacgccgcgc
     4141 ccagcgcatc aaggtcgagc gccagcatgc ccggcgcgtg ggcgccgtcg acgagcgtgt
     4201 cgatgccgcg ttgggccagc tccggcacca gccgctcaac aggcaggcgg atgccagtcg
     4261 ggctggtcac gtggctgatc aggaccagcc gtgtgcgctg agtgacgcgc tcgaggagcg
     4321 catcggccat ctcgtccggg gacatcgttg ggaacggcaa cggcaccacg accaccgtcg
     4381 cgccgtgcct ctttgccacc cgccgggcgg cgttgaggca ggcgttgtac tcgtggtcgg
     4441 tcgacaggat ctcgtcgccc ggcgcgaatt cgagcgacgc cagcacggta ttcacgcccg
     4501 tcgtggcgtt gggcacgaac gccaggtcgg aggcctgggc gttgagcaac tcgcccagcc
     4561 gttcgcgggc acgcgcgagg tgaccttcca gctcgacgtc gtggaactgg acagggtgcc
     4621 tttccatcct cgtccgccac tcatcctgga cgcgcagcac ggcccccggg cacgagccga
     4681 aggcgccgtg gttgaggtac gtcacatcgg gatcgagcag ccaatgccgc ttcagaccct
     4741 gcgtcgtcac gggcggtgcg gtcacggcgc gaagactagc ggccgctacg atcgcgccca
     4801 tgtgtggccg catgacgatg cgcaccgacg tgtcggacgt cgcgctcatc ttcgatgccg
     4861 aggtgcgcga tcccggcgcg ttcgccgacc tggggccgcg ctacaacgtc gcgcccactc
     4921 agccgatcga ggtcgtcgtc cagcgcgacg atgggcgctt catcgagctc caccgctggg
     4981 gcctggtgcc ctcatacgcg aagagcgtca gcgccgggaa tcgactcatc aacgcgcgcg
     5041 ccgaaacgat cgcgaccacg cccgcgttcc gcgcgtcgtt cgcgcggcgg cgctgcatca
     5101 tcccggctga cggcttctac gagtggcggc gcgagccggg tggcaaacgg aagcaaccct
     5161 ttctcatcca cgccgcggac gatcggccgc tcgccttcgc cggcgtgtgg tcgccctgga
     5221 aggacccggc cacgggcgag tgggtgctga gcgcggccgt cgtcacgacg agcgccaatg
     5281 cggtggtggg ccagctccat gaccggatgc cggtcatcct cgagcctttg gcatggccca
     5341 tctgggccga tcccgagatc cgcgatcccg gcctgctgac cgacctcctc caaccagccg
     5401 cggacggact gctgatgctg accgc
//
LOCUS       DATBRX010000105         6246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3161019, whole genome shotgun sequence.
ACCESSION   DATBRX010000105 DATBRX010000000
VERSION     DATBRX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3161019"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VIF08_03860"
     CDS             <1..302
                     /locus_tag="VIF08_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY8135151.1"
                     /translation="RWGRALYGGDVLSDASLATMTTFTFHPEKQGGYYGMGTRMRKFG
                     AFSMEGHTGTLRAFNAAMWYLPDLDLTVVVMTNRTGVDANALTDALLAVALPALD"
     gene            complement(299..1852)
                     /locus_tag="VIF08_03865"
     CDS             complement(299..1852)
                     /locus_tag="VIF08_03865"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY8135152.1"
                     /translation="MSTDLRALAELLRSRGMLRSVAGSVEGVEVGRVQFDSRDISDGD
                     LFVAVTGAERDGHEFCAAAVERGAAALIVERPLPDIQVPQLVVTSSRGAMALAAAWSH
                     GFPSRELGVIGITGTDGKTTTSYLVRAMLGECGYPAGLLTTVEAVVGGESRGYLGHTT
                     PEAPVIQADLRAMVDAGDRFAVVETTSHGLALERVAEVAYDIAVLTNITHEHLDLHGT
                     YEAYVAAKRSLFSRLALSDANPDKGYGKHAVVNARDAEGASFAAAARAAGATVLTYSA
                     GDDIAADICGTDVRDLPTGVALRVRTARWEDEVEVHLAGSFNVHNALAAIGVGEALGL
                     DPAAMRAGLAHVRQVIGRMQPVDAGQPFDVYVDFAHTPGALAATIAALAPVAAARGGG
                     VISLFGSPGSRDIAKRPMMGAAAAQHSRVVIVTADDPRNEDPAAIAEEIAAGALEAGK
                     TRDADLLIIPDRRAAIRRAFEIARPHDIVLLAGKGHEPTIALKGGPEPWDEAAIAAET
                     LSELGYSRS"
     gene            complement(1852..3483)
                     /gene="murJ"
                     /locus_tag="VIF08_03870"
     CDS             complement(1852..3483)
                     /gene="murJ"
                     /locus_tag="VIF08_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY8135153.1"
                     /translation="MSGRTLARAGMIVAGAYFVSRILGYVRVVVITNEFGASAQLDAY
                     FAAFRVPDTIFQLVAAGAFGSSMVPVLAGIFAKGEDDRGWRVVSTVINLMLIVLATLA
                     TIVAVFAPAIVPLITPGFDAVNTELTIRLTRILLMSPVLLALAAVASSSLNVRGRFAS
                     AAVAPSLYNVSIILGAVLLGPVLGVEGLAVGVVIGSLLHLAIQIRPLIRERFRLTLKI
                     DLSDPAVRQILTLMVPRAIGLGANQIVFMVATMLATGVGVGAVTSYNVAMTLLQIPLG
                     TISFPMSLVLMPTLSRAAALGSTREWAQLLVRSIRLIAWIMFFITALGIVLRRQGVTL
                     LFDPGLNEQAIALTADTLSFMLLGLTALSLVIILARAFYSGQDTRTPVFTAFVDLAVA
                     ITLAAALVGSMGLSGIALGLAAGAWAEAIMLAVLLWRRMPAMGLGSVIRPLLLFAIGA
                     IIAGVVMFFAVRLTDPLIGTSPGKVLLLAQILTVSAVGGFTYILYTRLLGIPELGQAI
                     SLLRSSLRRGDRQGGDQTDRQTDSELTGGPPGMAE"
     gene            complement(3480..4424)
                     /locus_tag="VIF08_03875"
     CDS             complement(3480..4424)
                     /locus_tag="VIF08_03875"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEY8135154.1"
                     /translation="MKGRSAGGSNGAEALDSFLRRLAARDTSEHTRRSYRTAITQYLD
                     FLEARAVDWHRPPRPLIRGYMAELAERPLAKRSISSRLAALRSFYRWARREGIVDADP
                     WSGALTPRQPRRLPQVLGTDQVEDLLDAAPHSSARPALALRDRAIIETAYAGGLRISE
                     LAAARLADLDLGRGELRVLGKGRKERITLLGGPAREALSDYLREGRPELLATGTMAES
                     GVVFLNSKGAPLGVRGLRYRLARLMLAAGLPEGTSPHTLRHSFASHLLEGGADLRVVQ
                     ELLGHASLGTTQVYTHVTPGRLRSAYRAAHPRSSQPAK"
     gene            complement(4421..>6246)
                     /gene="topA"
                     /locus_tag="VIF08_03880"
     CDS             complement(4421..>6246)
                     /gene="topA"
                     /locus_tag="VIF08_03880"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY8135155.1"
                     /translation="ETFNAELVRIDGQTIARGEDEKGLRIGDEATANEHATALREKRP
                     QVGGISVKQSRRRPAPPFTTSTLQQEASRKLSFNPKRTMRVAQDLYEGVDLADGRTGL
                     ITYMRTDSPQMATVAMAEARDVVAGRYGERYVVSGGRNYDAKSKGAQEAHEAIRPTSF
                     ARDPDSLEHVLNPEQLRLYRLIWQRAIASQMPDKELETTTVELTADKYDLRASATKTT
                     FDGFAAVYTEGYDDSAEEAESQLPELAQGERTNVVDVTPEQHFTQPPPRYTEASLIKA
                     LEERGIGRPSTYAATISTIIDRGYVVVVERRLRPEHVAEVVTDLLVEHFNEFVDYDFT
                     ARMEDDLDQVAEGKRGWVPMLHEFYDPFAKLVELKKAELKRSDFTTEETDEVCSEGHP
                     MVIRLGRTGRFLACSKYPEHKETRPLPGEEPEAVAIEGVGETCPECGEGTLVGKRGRF
                     GPFAGCSRYPDCKYIRKTGPPPPDQLPFDVVCPKCNEGHLKARRARRTGSVFYGCSRY
                     PKCDFTSSREPTGALHDADDGPVAREGESGGICLKCGAPVELPAKIEPGMKLAGGEPN
                     PEALKPARRGGGRAGGARGGARRGGATKRGSTTGRRTPRAA"
BASE COUNT          965 a         2178 c         2188 g          915 t
ORIGIN      
        1 ctcgctgggg acgggcgcta tacggcggtg atgtcctctc tgacgcctcg ctggccacga
       61 tgaccacctt caccttccac cccgagaagc agggcggcta ctacggcatg ggcacccgca
      121 tgcgcaagtt cggggccttc agcatggagg gccacaccgg cacgctgcgc gccttcaacg
      181 cggccatgtg gtacctgccc gatctggatc tcacggtcgt ggtcatgacc aaccgcacgg
      241 gcgtcgacgc caatgcgctg accgacgcgc tgctcgcggt cgcgctcccg gcgctcgact
      301 aggagcgcga gtagccaagc tcgctgagcg tctctgcggc gatcgcggcc tcgtcccagg
      361 gctccggccc gcccttcagc gcgatcgtcg gctcatgacc cttgcccgcg agcagcacga
      421 tgtcgtgcgg ccgcgcgatc tcgaacgcgc ggcggatcgc ggcgcggcgg tcggggatga
      481 tcaagaggtc ggcgtcgcgc gtcttgccgg cctccagcgc gcccgcggca atctcctccg
      541 cgatcgctgc cgggtcttcg ttgcgcgggt cgtcggcggt gacgatgacg acgcgcgagt
      601 gctgcgccgc ggccgcgccc atcatgggtc gcttggcgat gtcgcgcgag ccgggcgagc
      661 cgaagagcga gatcacgcct ccgccccgag cggcggcgac gggcgccagc gcggcaatcg
      721 tcgcggcgag ggcacccggg gtatgggcga agtcgacata cacgtcgaac ggctggccgg
      781 cgtcaacagg ctgcatccgg ccgatgacct ggcgcacgtg tgcgagcccg gcgcgcatcg
      841 cggccggatc gaggcccagg gcctccccca cgccgatcgc ggcgagcgcg ttgtgcacgt
      901 tgaagctccc cgcgaggtgc acctcgacct cgtcctccca gcgcgcggtc cggacacgca
      961 gcgcgacgcc cgtgggcagg tcgcgcacgt ccgtcccaca gatgtccgcc gcgatgtcgt
     1021 cgcccgccga ataggtcagc acggtcgccc cggcggcgcg cgccgcggcc gcgaacgagg
     1081 cgccctccgc gtcgcgcgcg ttgaccaccg cgtgcttgcc gtagcccttg tcagggttcg
     1141 cgtcgctcag cgcgagccgc gagaaaagcg accgcttcgc ggccacataa gcctcgtacg
     1201 tgccgtgcag gtcgagatgc tcatgggtga tgttggtcag cacggcgatg tcgtacgcga
     1261 cctccgcgac gcgctccagc gccaggccgt gcgacgtcgt ctcgacgacc gcgaatcgat
     1321 cgccggcatc gaccatcgcg cgcagatcgg cctggatgac cggtgcctcg ggggtcgtgt
     1381 ggcccaggta gccccggctc tcgccgccca ccacggcttc gaccgtggtc agcaaccctg
     1441 ccgggtagcc gcactcgccg agcatcgcgc gaaccaggta ggacgtggtc gtcttgccat
     1501 ccgtgccggt aatgccaatg acgccgagct cgcgactggg aaagccgtgc gaccacgcgg
     1561 cggcgagagc catcgcgccg cgcgacgagg tgacgacgag ctgcggcact tggatgtcgg
     1621 gcagcggccg ctcaacgatg agcgcggcgg cgcctcgctc taccgccgcg gcgcagaact
     1681 cgtgtccgtc gcgctcggcg cccgtgaccg caacgaacag gtcgccgtca gagatgtcac
     1741 gcgagtcgaa ctggacgcga ccgacctcga cgccctcgac cgaacccgcg acggaacgca
     1801 gcatgccgcg cgaacgcagg agctcggcca atgcccgcag gtcggtgctc atcattcggc
     1861 catcccgggc ggcccacccg tcagctccga atcggtttgc cgatcggtct ggtccccgcc
     1921 ctgacggtca ccccggcgca gcgaggagcg cagcagggaa atcgcctgac ccagctcggg
     1981 gatgcccagc aatcgggtgt acaggatgta cgtgaagccg ccaacggcag aaacagtgag
     2041 gatctgggcg agcagcagga ctttgcccgg cgaggtgccg atcagcgggt cggtcaggcg
     2101 cacggcgaag aacatcacca cgccagcgat gatcgcgccg atcgcgaaca gcagcagcgg
     2161 ccggatcacc gacccgaggc ccatggcggg catccggcgc cagagcagga cggcaagcat
     2221 gatcgcctct gcccacgccc cggccgccag tcccagcgcg atgcccgaca ggcccatgct
     2281 cccaaccagc gccgcagcga gggtgatcgc cacggccagg tcgacgaatg cggtgaagac
     2341 cggcgtgcgc gtgtcctggc cgctgtagaa ggcgcgcgcc aggatgatca ccagcgagag
     2401 tgcggtcagg ccgagcagca tgaatgacag ggtgtcggcg gtgagcgcga tggcctgctc
     2461 gttgaggccg ggatcgaaga gcagggtcac accctggcgg cgcagcacga tcccgagcgc
     2521 cgtgatgaag aacatgatcc aggcgatgag gcgtattgaa cgcaccaaca gctgcgccca
     2581 ttccctggtc gagcccaggg cggcggcgcg cgacagcgtc ggcatcaaca ccagcgacat
     2641 cgggaagctg attgtgccca gcgggatctg gagcagcgtc atggccacgt tgtagctggt
     2701 cacggcgccc acgcccacgc ccgtggcgag catcgtcgcg accatgaaca cgatctggtt
     2761 cgcgcccagg ccgatcgccc gcggcaccat cagcgtgagg atctggcgca cggccgggtc
     2821 ggacaggtcg atcttcagcg tcaggcggaa gcgctcacgg atcaagggcc ggatctggat
     2881 tgcgaggtgg agcagtgagc cgatcacgac gccgactgcc aatccttcga cgcccagcac
     2941 cggcccgagc agcaccgcgc ccaggatgat cgagacgttg tagagcgacg gcgccactgc
     3001 cgccgacgcg aagcggccac gaacgttgag cgacgacgac gcgacggccg cgagcgcgag
     3061 cagcaccggc gacatcaaca ggatgcgcgt gaggcggatc gtcagctctg tgttgaccgc
     3121 gtcgaagccg ggggtgatga gcggcacgat cgcgggcgcg aacacggcga cgatcgtggc
     3181 cagcgtcgcg aggacgatga gcatcaggtt gatgaccgtc gagacgacgc gccagccgcg
     3241 gtcgtcctcg cccttcgcga agatgcccgc gagcactggc accatcgacg agccaaaggc
     3301 gcccgcggcc acaagctgga agatcgtgtc cggtacccgg aacgcggcga aataggcgtc
     3361 cagctgggcg ctcgccccga actcgttggt gatgaccacg acccggacgt agcccaggat
     3421 gcgcgacacg aagtaggcgc cggcaacgat catgccggcg cgggccaagg tgcgaccgct
     3481 catttcgctg gttgtgacga gcgcggatgg gcggcgcgat acgcgctgcg caaccgcccc
     3541 ggggtgacgt gcgtgtacac ctgggtcgtg cccaggctgg catggcccag cagctcctgg
     3601 acgacgcgca ggtcggcgcc gccttcgagc aggtggctgg cgaacgagtg gcgcagggtg
     3661 tggggggacg tgccctcagg cagacccgcg gcgagcatca gccgcgccag gcggtagcgc
     3721 agccctctca ccccgagcgg cgcgcccttg ctgttgagga atacgacgcc cgattcggcc
     3781 atcgtgccgg tggcgaggag ctccggccga ccttcgcgca ggtagtcgct cagcgcttcc
     3841 ctggccggcc ctcccagcag cgtgatgcgc tccttgcggc ccttgcccag cacgcgcagc
     3901 tcgccgcggc ccaggtcgag atcggccagg cgcgcggcgg caagctcgct gatgcgcagc
     3961 ccaccggcat acgcggtctc gatgatcgcg cggtcgcgca gggcgagcgc cgggcgcgcc
     4021 gatgaatgcg gcgcggcatc gagcaggtcc tcgacctggt cggtgccaag gacctggggc
     4081 aggcgtcgcg gctgccgcgg cgtcagcgcg cccgaccacg gatctgcgtc aacgatgcct
     4141 tcgcggcgcg cccagcggta gaacgagcgc agagcggcca ggcggctgct gatggagcgc
     4201 ttggccagcg gtcgctcggc gagctccgcc atgtaaccgc ggatgagcgg ccgcggcggt
     4261 ctatgccagt ccacggcacg tgcctccagg aagtcgagat actgggttat cgccgtgcga
     4321 taggagcgcc gcgtgtgctc cgacgtgtcg cgcgcggcga gtcggcgaag gaaggagtcg
     4381 agcgcctcgg cgccgttcga tccgcccgcg gatcgcccct tcacgccgcg cgcggggttc
     4441 gacgcccggt cgtggacccg cgcttggttg ctcccccgcg acgcgcccca ccacgcgccc
     4501 cacccgcccg gccgccaccg cggcgcgccg gcttgagcgc ttccggattc ggctcgccgc
     4561 cggcgagctt catgcccggc tcgatcttgg ccgggagctc caccggcgcg ccgcacttca
     4621 ggcagatgcc gccgctctcg ccctcgcgcg cgaccggtcc gtcgtccgcg tcatgcaggg
     4681 cgcccgtcgg ctcgcgcgac gaggtgaagt cgcacttggg atagcgcgag cagccataga
     4741 agacggagcc ggtccgtcgc gctcgccgcg ccttgagatg tccctcgttg catttggggc
     4801 agaccacgtc gaacggcaac tgatcgggcg gcggcgggcc ggtcttgcgg atgtacttgc
     4861 agtcgggata gcgcgagcag ccggcgaacg gcccaaagcg gccgcgtttc ccgaccagcg
     4921 tgccctcgcc gcattccgga cacgtctcgc ccacgccttc gatcgcgacc gcctccggct
     4981 cctccccagg gagcggccgc gtctccttgt gctcggggta cttggagcat gcgaggaagc
     5041 gcccggtgcg gccgagtcgg atcaccatgg ggtggccctc cgagcacacc tcgtcggtct
     5101 cctcggtggt gaagtcggag cgcttgagct cggccttctt gagctcgacg agcttcgcga
     5161 acgggtcgta gaactcgtgc agcatcggca cccagccgcg cttgccttcc gcgacctggt
     5221 ccaggtcgtc ctccatgcgc gcggtgaagt cgtagtcgac gaactcgttg aagtgctcga
     5281 ccagcaggtc ggtcacgacc tcggcgacat gctccggccg caaacgccgc tcgacaacaa
     5341 cgacgtagcc gcgatcgatg atggtcgaga tggtggcggc atatgtcgac ggccggccga
     5401 tgccgcgctc ctcgagcgcc ttgatcagcg acgcttccgt gtagcgcggc ggcggctggg
     5461 tgaagtgctg ctccggcgtg acgtcgacga cgttcgtccg ctcaccctgc gcgagctcgg
     5521 gcagctgtga ttcagcttcc tcggcgctgt cgtcatagcc ctctgtgtag accgcggcga
     5581 agccatcgaa ggtggtcttg gtggcggagg cgcgcaggtc gtacttgtcg gccgtcagct
     5641 cgacggtggt cgtctcgagc tccttgtcgg gcatctgcga cgcgatcgcg cgctgccaga
     5701 tgagccggta cagccgcagc tgctccggat tgaggacgtg ctcgagcgag tcggggtcgc
     5761 gggcaaacga tgtgggacgg atcgcctcgt gcgcctcctg ggcgcccttg gacttggcgt
     5821 cgtagttacg cccaccgctc acgacgtaac gctcgccata gcgccccgcc acgacgtcgc
     5881 gcgcctcagc catcgcgacg gtcgccattt gcggcgaatc ggtgcgcatg taagtgatga
     5941 gtccggtgcg cccgtcggcc agatcgacgc cctcgtacag gtcctgcgcg acgcgcatcg
     6001 tgcgcttggg gttgaagctc agcttgcggc tcgcctcctg ctgcagagtg ctggtcgtaa
     6061 agggcggcgc cgggcggcgg cgactctgct tgacgctgat gccgccgacc tgcggccgct
     6121 tctcgcgcag cgcggtcgca tgctcgttgg cggtcgcctc atcaccgatg cgcaggccct
     6181 tctcgtcctc gccccgggcg atggtctggc cgtcgatgcg gaccagctcc gcgttgaagg
     6241 tctcgc
//
LOCUS       DATBRX010000106         5216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2379659, whole genome shotgun sequence.
ACCESSION   DATBRX010000106 DATBRX010000000
VERSION     DATBRX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379659"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VIF08_03885"
     CDS             complement(<1..1537)
                     /locus_tag="VIF08_03885"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY8135156.1"
                     /translation="MMVARPVIDLADRFWQWFLARNPVYATVLGDERYDDRLRDPGPD
                     ARAEEIAALKGFLRDASEIDTVGLDQEDSITLDMLEVVCRIQLREHELNPHHFEALDQ
                     MAGPQGLAGDLSRFQRLDTPERVDRLIRRLELFPEFLAKHRANMLDGIAAGRTASAVV
                     VERVIEQTRRAAAATADESPILLAHPELDDATRERIRAAIQDWVVPALADHVSALEGY
                     APYARQSEGVWALPDGREYYETAILASTTLEETPEALHEYGRSQLDAIRRETEGIARE
                     LKFADVAAMRAALNGDPANYAASGAEIVERATAQIAKANAAAPRWFGRLPVAPCEVRA
                     VEPYQEKEAPPAFYFAPAPDGSRPGIYFVNTYQPEQRPLYRLATTTYHEATPGHHFQI
                     AIEGELTNQPEFRRFGSRLTGAAYAEGWGLYSERLADEMGLFESAWERLGMLDAQAWR
                     AARLVVDTGMHAFKWTRAQSVELLGSIGLSQLEAETETDRYICWPGQALAYMTGMREI
                     MALR"
     gene            complement(1581..4310)
                     /locus_tag="VIF08_03890"
     CDS             complement(1581..4310)
                     /locus_tag="VIF08_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135157.1"
                     /translation="MADPLSSLIRPDAGALLPAFERFAPPPPDNVVSAELAARSAPGD
                     IVIDIHGRGGWIARNAISALRRVYVCESTALTRLLAEVVLRPPDLRHFDAGLATLATL
                     PRGDGEFRRVLEAMFASRCPTCGRPVIVDEFLWETGATGPDRKVFRCAFCKEQARGEP
                     RVVPVDADDLAHAHAVGERPAAYAALRARFPAPDASAALPDELLALYTPRTLIALEAL
                     ISRLEIGLRAAPVDAGLRLGLVHALLSMSRLNGYPGRTAALRIRHGHVQQPNSRAWRE
                     RNPWLAFEEGCRLMRDFITTLEVGAGSFQPRTGEDMEALSDGTANVVLRTGSAATPGN
                     EPQFSSRRPVLPGRLDPRSRVRLVLTSPPARWSIENASLAYLGTALVVGRQAATDLPL
                     EWIFGPPPRNDRGREATALRHSLLAVRPVLARDAKAVVVLDRGGASGLVAGVLGGIGA
                     GFRLNSALLAESNNQVTGVLEFDVAIPADEPDDHPELAGLPRADPDGPFALADVENAV
                     TALAVTVLQARGEPASAERLLGEVLIGLDRLGHLRRLVGTQTFNESEAATESMEIEVT
                     DQPGDFLASAEAEAAENAPAEIATTETAEGVREFAPDRPPEWVVGAGSATDHVRLLME
                     IVLGELRRPDNPRMAEIEPGRWWLRADKDLAQARPPLSDRLEWAVYGLLSTSSAMEEA
                     TFFERVARMFRGHDTPDPELVRKVLESYRDPEGHQVMLRSIDQLTTRHAEHGEMIGIL
                     TEYGHRLGLRVHVGQKECRRAYRGGVVGDLLSDEEQRAYLPLIAAGDVATLEALDCMW
                     YLRGKATFLFEVEWTALLTEPLLKRGPRIPAADTVVRFLVVPSARVELIRYKLAHSPL
                     LRRALDEQNWHILKSEHLRRLVAREDADLDMLGPMLGLDPEIEREAEQLPLFG"
     gene            4483..4926
                     /locus_tag="VIF08_03895"
     CDS             4483..4926
                     /locus_tag="VIF08_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8135158.1"
                     /translation="MANDDYVPPPHKARLAAAPSFFIPPPFGWEHPVTGRRGWQPSFE
                     LIAFGMYVKRARYLANMTQTKLEQLSGVDQGQISRLERALAPWTRVEDLVPLGTALGR
                     AFPLGFCPHEHWCEWQPAPKPPPEPTWQEKWQEAERRYRSHDPIE"
BASE COUNT          797 a         1820 c         1827 g          772 t
ORIGIN      
        1 ggcgcagagc catgatctcg cgcatgccgg tcatgtacgc cagcgcctgg ccaggccaac
       61 agatgtaccg atcggtctcc gtctcagcct cgagctgcga caggccgatg ctgcccagca
      121 gctcgaccga ctgggcgcgc gtccatttga aagcgtgcat gccggtgtcg acgacgaggc
      181 gcgcggcgcg ccaggcctgc gcgtcgagca tgccgagtcg ctcccaggcg ctctcgaaca
      241 gccccatctc gtcggccagg cgctcgctgt acaggcccca accctcggca taggcggcgc
      301 ctgtcagtcg agagccgaag cgccggaact cgggctgatt ggtcagctcg ccctcgatcg
      361 cgatctggaa gtggtgaccc ggcgtggcct cgtggtaggt ggtcgtcgcg agtcgataga
      421 gaggccgctg ctcgggctgg taggtgttga cgaagtagat gcctggccgc gagccgtcgg
      481 gcgccggggc gaagtagaac gccggcggcg cctccttctc ctggtacggc tctacggcgc
      541 gcacctcgca gggcgcgacg ggcaaccgcc cgaaccagcg cggcgccgcg gcattggcct
      601 tggcgatctg cgccgtcgcg cgctcgacga tctcggcgcc cgatgcggcg tagttggccg
      661 ggtccccgtt cagcgcggcg cgcatcgcag cgacatcggc gaacttgagc tcgcgcgcga
      721 tcccctccgt ctcgcggcgg atcgcatcga gctgcgaccg gccgtactcg tgcagcgcct
      781 caggcgtctc ttcgagcgtc gtcgaggcga ggatcgcggt ctcgtaatac tcgcgcccgt
      841 cgggcagcgc ccagacgccc tcgctctggc gtgcgtaggg tgcgtaaccc tcgagcgcgg
      901 acacgtggtc ggcgagggcc gggacgaccc agtcctggat cgcggcgcga atgcgctcac
      961 gcgtcgcgtc atcgagctcc gggtgggcga gcaggatggg cgactcgtcg gccgtcgcgg
     1021 ccgcggcgcg gcgcgtttgc tcgatcaccc gctccacaac gaccgcggag gccgtccggc
     1081 cggcggcgat gccgtcgagc atgtttgcgc ggtgtttggc caggaattcg gggaagagct
     1141 cgagccggcg gatgagccgg tcgacgcgct cgggggtgtc gaggcgctgg aagcgcgaca
     1201 gatcacccgc caggccctgc ggcccggcca tctggtcgag tgcctcgaag tggtgcgggt
     1261 tgagctcgtg ctcgcgcagc tggatgcggc acacgacctc gagcatgtcg agcgtgatgg
     1321 agtcttcctg gtccaggccg accgtgtcga tctcggacgc gtcgcgcagg aagcccttca
     1381 gcgcggcaat ctcctcggcg cgcgcgtcgg gccccgggtc acgcagccgg tcgtcgtagc
     1441 gctcgtcgcc cagcaccgtg gcatagaccg ggttgcgcgc caggaaccac tgccagaagc
     1501 gatcggcaag gtcgatcaca ggccgcgcca ccatcacgtc ggtcaactgt ccacctccac
     1561 gcgcgattga ctgtcagtcg tcagccgaaa agcggcagct gctcggcttc tcgttcgatc
     1621 tccggatcca gcccgagcat cggcccgagc atgtccaggt cggcatcttc gcgcgccacc
     1681 aggcggcgca ggtgctccga tttgaggata tgccagttct gctcgtcgag cgcccgtcgc
     1741 agcagcggcg agtgagcgag cttgtagcgg atcagctcga cccgcgcgct gggcacgacc
     1801 aggaagcgga ccacggtgtc ggccgcggga atgcgcggcc cgcgtttgag cagcggctcg
     1861 gtcagcagcg ctgtccattc cacctcgaac aggaacgtcg ccttgccgcg caggtaccac
     1921 atgcaatcaa gcgcctcgag ggtggccacg tcgccggcgg cgatcagcgg caggtaggca
     1981 cgctgctcct catcgctgag caggtcaccg accacgccgc cccgatatgc gcgccggcac
     2041 tccttctggc cgacgtggac gcgcaggccc aggcgatggc cgtactcggt caggatgccg
     2101 atcatctcgc catgctccgc gtggcgcgtt gtcagctggt caatggagcg cagcatgacc
     2161 tggtggcctt cggggtcgcg gtagctctcg aggaccttcc ggacgagctc cggatcgggc
     2221 gtgtcgtggc cgcggaacat gcgcgcgacg cgctcgaaga aggtcgcttc ctccatcgcc
     2281 gatgacgtcg acagcagacc gtagacggcc cactcgagcc gatccgacag gggcggccgt
     2341 gcctgcgcga ggtctttgtc ggcgcgcagc caccagcgac cgggctcgat ctcggccatg
     2401 cgcggattgt ccgggcggcg cagctcaccc agcacgatct ccatcagcag ccgcacgtga
     2461 tcagtggctg agccggcgcc gaccacccac tctggcggcc ggtcgggcgc gaactccctg
     2521 acaccctctg ccgtctccgt agtggcgatc tcggccgggg cgttctcagc cgcctcggcc
     2581 tccgcggagg cgaggaagtc acccggttga tcggtgacct cgatctccat cgactcggta
     2641 gccgcctcgc tttcgttgaa ggtctgcgtg ccgacgaggc gtcgcaggtg gcccaatcgg
     2701 tcgagcccaa tcagcacctc acccagcagc cgttcggcgc tggccggctc accacgcgcc
     2761 tgcaggaccg tgaccgcgag tgccgtgacc gcgttctcga cgtcggccag ggcgaacggc
     2821 ccgtccgggt cggcccgcgg cagaccggca agctcggggt ggtcgtcagg ttcatcggcc
     2881 gggatcgcga cgtcgaactc caggacgccg gtgacttgat tgttcgattc ggcgagcaat
     2941 gccgaattga gccggaagcc ggcaccgatg cccccgagca cgcctgccac gagcccggac
     3001 gctcccccgc ggtccagcac gaccactgcc ttggcgtcgc gcgccagcac cggtcggacc
     3061 gcgagcagcg aatggcgcag cgccgtggcc tcgcgcccgc gatcgttgcg cggcggcggt
     3121 ccgaagatcc actcgagcgg caggtcggtc gcggcctgcc ggccgaccac cagcgccgtc
     3181 ccgagatagg ccagcgacgc gttctcgatc gaccagcgcg cgggcggcga ggtgagcacc
     3241 aggcgcaccc gcgagcgcgg atcgagccgg cccggcagga ccgggcggcg cgaggagaat
     3301 tgcggctcgt tgccgggtgt cgccgcggag cccgtgcgca gcaccacgtt ggccgtgccg
     3361 tcgctcagcg cctccatgtc ctcgcccgtg cgcggctgga atgagccggc gccgacctca
     3421 agcgtggtga tgaagtcgcg cattagccgg cagccctcct cgaaggcgag ccacggattg
     3481 cgctcgcgcc aggcacgcga gttgggctgc tggacgtggc cgtggcggat gcgcagcgcg
     3541 gccgtgcggc cgggatagcc gttgagccgg ctcatcgaca gcagggcgtg caccaggcca
     3601 aggcgcaggc cggcgtcgac gggcgccgcg cgcaggccga tctcgagccg gctgatgagc
     3661 gcctcgagcg cgatcagggt gcgcggcgtg tacaacgcga ggagctcgtc gggcagggcc
     3721 gcgctcgcat cgggggcggg gaagcgggcg cgcagcgccg cataagcggc cggccgctca
     3781 ccgacggcat gggcgtgcgc aaggtcgtcg gcgtccaccg gcacgacccg cggctccccg
     3841 cgggcctgct ccttgcagaa ggcgcagcgg aacaccttgc gatccggtcc ggtcgcgccc
     3901 gtctcccaca ggaactcgtc gacgatgacc ggccggccgc acgtcggaca gcgcgacgcg
     3961 aacatcgcct cgagcacgcg ccggaactcg ccgtcgccgc gcggcagcgt tgcgagcgtc
     4021 gccagtccgg cgtcgaagtg acgcaggtcg ggcggccgca gcacgacctc ggccagcagc
     4081 cgtgtgaggg cagtcgattc gcacacgtac acgcggcgca gcgcgctgat cgcgttgcgg
     4141 gcgatccacc cgccgcggcc gtgaatgtcg atcacgatgt cgcccggggc gctgcgcgcc
     4201 gcgagctccg ctgagacgac gttatccggt ggaggtggcg cgaaccgttc gaacgcgggc
     4261 agcaacgctc ccgcgtcggg ccgaatgagc gaggagagtg ggtccgccat cgatgccgag
     4321 tctatccacg caccggccgc gcgccttacc actcgtgccg ccagcgcggg gttcatgtag
     4381 cgacaggcgt ggatatgaac cgaccggcgt gaatatgtgg gcgaaagtgg gcggtgatcc
     4441 gccggcaacc gcgggcgaag gcgacctcat cacactcgag ccatggcaaa tgacgactac
     4501 gtgccgccac ctcacaaggc gcgcctggca gccgcgccca gcttcttcat ccctcccccg
     4561 ttcggatggg agcacccggt caccggtcga cgcggctggc agccaagctt cgagctgatc
     4621 gcgttcggga tgtacgtcaa gcgtgcgcga tacctggcga acatgaccca gaccaagctg
     4681 gagcagctat ccggcgtgga tcagggccag atctcgcgcc tcgaacgggc tctggcgccc
     4741 tggacgcggg tcgaggatct agtgccgctc ggcacggccc tcggacgcgc attcccgctc
     4801 ggcttctgcc ctcacgagca ttggtgcgag tggcagccgg cacccaagcc accgccggag
     4861 ccgacgtggc aggagaagtg gcaagaggcc gagcggcgct atcgaagcca cgaccccatc
     4921 gagtagcggg agccacgggc aggcagttgg ctctcgccta cgacatattc aggccaatcg
     4981 acacatatct acgccggtcg gtaaatgagg cgatggctgg cgtgacgaga ggcggtcaaa
     5041 acgatccgcc ctgcgcgagc cggcaggcag ccgggacgcc tactcctcgg agtcgtctga
     5101 ctcttcgacc ggctcctctt cgacgaatgc ctcgtcctcg gtcacctcgg cgggcaggac
     5161 gcgaccgatg tcctcgatct cgtcctcttc ctcgtcctcc tccaggtcgt cctcga
//
LOCUS       DATBRX010000107         8067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_816108, whole genome shotgun sequence.
ACCESSION   DATBRX010000107 DATBRX010000000
VERSION     DATBRX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816108"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VIF08_03900"
     CDS             complement(<1..803)
                     /locus_tag="VIF08_03900"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY8135159.1"
                     /translation="MSLGSAIPTATSAPDLVRRPSKTDSLRPTYGFDDVSLAPGTETL
                     DPADVDTSVHFGGIDLAIPILAAAMDAVVDPSFAGQLAKLGGLAVLNLEGVQTRYEDP
                     APILQRIATLPDDQVQDALAEAYAAPIQEALIAQRIADIKAAGSPAFVAATPAAARRL
                     GPFCAEHGADMFLVQSQVSSARHLAARYEPLALSDFTRYMPIPVAVGNTTSYQAAIEL
                     MRQGAAAVFVGVGPGAACTTRDVLGIGVPQITAIADVAAARDDYFDQSGR"
     gene            900..1910
                     /locus_tag="VIF08_03905"
     CDS             900..1910
                     /locus_tag="VIF08_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY8135160.1"
                     /translation="MHPPDDGRLVTLDDIGEARSRIAGVAFRTPLVPFREDAWLKPES
                     LQPTGSFKIRGAYNTLAQMTPDERARGVVTHSSGNHAQAIARAARLLGIRALVVMPNN
                     APAVKVAGVRADGAEIEFVGPHNEERVARAHQIAERDGMVLVPSANHPSVIAGQGTIG
                     LEIVEQLLEMSWSHLPTASEAPVVLVPVGLGGLAAGVATAIKSAPAWAGATGRVYGVE
                     PEVAADTRDSLAARELTPWPSARTSSTIADGLRGESPSPIPFRHLLAHLDGVVTVSEA
                     EIGAAVAIAAREARLVLEPSGAVSLAALLNRRAELPEGPVVCVLSGGNVDPARYLEFL
                     RV"
     gene            1959..2975
                     /locus_tag="VIF08_03910"
     CDS             1959..2975
                     /locus_tag="VIF08_03910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8135161.1"
                     /translation="MDRSNPGFSLLEAARIVARGPELDTKLDALAGQVLSAAGATAAV
                     IYLLDPVANVLVPAAQSGLRGKMITARAEVPLDDPEELVAKVARERRQMSISGREAAS
                     AFTGQHPEWHDLVAVPLVAADETGGEDAEGVLLAAYEAAPPEGSDDLLTALADLSAIA
                     IRQARLQRALLERADWIGRLASTDPLTGLANRVTFERMLELELARATRQESQLSVLLF
                     DVDGFAQINSDAGSPAGDEVLRHVAATLADQVRLVDTVARYGGDEFALIAPGGGGEVV
                     GRRVRDALLKREAAGKPISVSVGAVVYPIDGATTTELLIAAANALAEAKRRGRGSIFV
                     ASGS"
     gene            complement(2972..3604)
                     /gene="tmk"
                     /locus_tag="VIF08_03915"
     CDS             complement(2972..3604)
                     /gene="tmk"
                     /locus_tag="VIF08_03915"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY8135162.1"
                     /translation="MTRLFITIEGPDGAGKSSQATLLAEHVRGLGKTVVLTREPGGTR
                     VGEGVRNVLLDREGEGHTAVSDALLYNAARHHSVIEVIRPALEHGDIVISDRYADSTL
                     AYQGYGGGAPLAELGAIQRLATGGLMPHRTVLIDVSVEAGLSRRHSGPAEGLNRFETS
                     SLHGTDFHQRVRDGYLQLAAADPERWRVVDGAADRDQVAARVWAAVQDLF"
     gene            complement(3617..4375)
                     /locus_tag="VIF08_03920"
     CDS             complement(3617..4375)
                     /locus_tag="VIF08_03920"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY8135163.1"
                     /translation="MNHAEAIELAGLYVLDALDPAERAQVDAHLASCPDAHDEFAEVG
                     AVAPALATLADPVGAPASLKNKVMADYRAGAGVRAWEPAPATTRPMQRSWLGWAAAAA
                     AVLVIAVTAGWAYVAQSNAALEAHRAQMIARAIDVMAAPGSSVAIMEGSATAAGASGF
                     AAFGADGAGYIVLIDMPVAPAGKTYQAWFIADGVPASAGLLSVDRDGYALMALSGRPG
                     TQVIALTVEPTGGSELPTSDPIAAGELRSNAAES"
     gene            complement(4372..4968)
                     /locus_tag="VIF08_03925"
     CDS             complement(4372..4968)
                     /locus_tag="VIF08_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8135164.1"
                     /translation="MSGPPNTDAADGGRASGPSDEALLERLAAGPDERALSDLYDRYQ
                     AQMYGLAMRITRDPALAQDAVQEAFVGVWRNAGRYAAGRSSVRTWMLSIAHHRAIDIV
                     RRRRPTSALPEIETAEPALTTPDVWPEVARTLDRDSVTAAMASLPDPQREAIQLAYFE
                     GLTQVEIAERTSTPLGTVKSRVRLGLLALRRALQEDGT"
     gene            5228..6262
                     /locus_tag="VIF08_03930"
     CDS             5228..6262
                     /locus_tag="VIF08_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEY8135165.1"
                     /translation="MRNSRQRAMAASIASALLILTLGLTPVVSGADHLDAPAVAAQGS
                     VDITDIYTFSANNGNSTVFIVGVNPGAGALPNSGETFGPGVDYKIKVDTNGDLKPDVT
                     YRYRFGTPNGMGVQTLKLWRNGSMLVNGWTDQNNLLPGGGRTTAGLFDDPFFFDLAAF
                     QGAVLMSGNGRTFCDGGTTDFFLGLNISAIVLRVPNSSLGGNGTNIGVWATTEAVRGG
                     KRVQLDQMGRPAINTVFNNQTAEHFDRDGFNRVQPSEMVAAGYRDHAEDVLTALGAAD
                     PAGLAAVLIPDVLTFQTGNMAGFLNGRRLANDVIDAELALVTNNGITTDCIGNDSAFP
                     GAWPYLAPAN"
     gene            6390..7715
                     /locus_tag="VIF08_03935"
     CDS             6390..7715
                     /locus_tag="VIF08_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135166.1"
                     /translation="MALVVVFAFQMWPRPVAAPPTNVTPITEGADTFTDVRPPGEVIA
                     LSGVDTKDTDRKIAFWQARIAANARDDISWTYLGDLFEIKGRQTGEVSNYLSARDAYA
                     TALDIAPGSAAAAIGAARIYSTLHDFLGAQAAATAVLEADPYATGALAILFDASLEMG
                     QLDIAQDALTLLLERTQTPAVVGRQARLAFVRGDTAGALALSNQAVLDATNAGDPVSS
                     VAFYQYAHAEYALLAGDLDGAQAGYEASLAALPGYPLALFGQGRVAYARGDVAHAIAL
                     LEAATAALPRPDMVAFLGDLYAIGGRPEDAATQYDTVEFIRGLAAAGSAKVYDREYVG
                     YLADHARDAALAVDLAAAELAARKDVYGYDALAWALHSAGKDAEALDNARLALASGTQ
                     DARLLIHAGLIELANGLDAEGQAHLRAGLALNPSFSPLVVEMARAQVAP"
     gene            7715..>8067
                     /locus_tag="VIF08_03940"
     CDS             7715..>8067
                     /locus_tag="VIF08_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135167.1"
                     /translation="MRRVVASLALSLAALALVVPATSAHPLGNFTINHYNGIRVAIES
                     VVIDHVTDFAEIPTFTERQAMDANGDGAISAAESTDYQLAACDGLASTLALSANGARL
                     ALTPIATGLHFSMGSG"
BASE COUNT         1220 a         2867 c         2739 g         1241 t
ORIGIN      
        1 cggccgctct gatcgaagta atcgtcgcgc gccgcggcga cgtcggcgat cgcggtgatc
       61 tgcggcacgc caatgccgag gacgtctcgc gtcgtgcacg cagctcctgg cccgacgccc
      121 acgaagaccg cggccgcgcc ctggcgcatc agctcgatcg cggcttggta gctcgttgtg
      181 ttgccgacgg ccacggggat gggcatgtac ctggtgaagt cgctcagcgc gagcggctcg
      241 tagcgcgccg ccaggtggcg cgcactggag acctgggact ggacgaggaa catgtccgcg
      301 ccatgctcgg cacagaaggg tcccaggcgg cgggcggccg ccggggtcgc cgcgacgaac
      361 gccggcgagc ctgcggcctt gatgtccgcg atgcgctgcg cgatgagcgc ttcctggatc
      421 ggcgccgcgt aagcctcagc gagcgcgtcc tggacctggt cgtcgggcag ggtcgcgatg
      481 cgctgcagga tgggcgccgg gtcctcgtag cgcgtctgga cgccctcgag attcagcacc
      541 gccaggccgc ccagctttgc gagctgtccg gcgaagctcg gatccacgac cgcgtccatt
      601 gccgcagcca ggatcgggat ggccaggtcg atgccgccaa agtgaacgga tgtgtcgaca
      661 tcggccggat cgagcgtctc cgtacccggc gcgagcgaga cgtcgtcgaa gccgtaggtg
      721 gggcggagcg aatcggtctt ggacggacgg cggacgaggt cgggcgcgct cgttgcggtg
      781 ggtatggcgc taccgagact catccgacga tggggacctc caactcgatt gcgacccgat
      841 tgcgactcgc ctgcgactcg cttgcaacac ggcttgccga gtgcgggcag tatagccaag
      901 tgcatccccc cgacgacggc cgactggtga cgctcgacga catcggcgaa gcgcgatccc
      961 gtatcgctgg cgtcgcgttc cgaaccccgc tggtcccgtt cagggaggac gcctggctga
     1021 agccggagag cctccagccg accggctcgt tcaagattcg aggcgcctac aacacgctcg
     1081 cccagatgac acctgacgag cgagcgcggg gcgtcgtcac ccattcctcc ggcaaccacg
     1141 cccaggcgat cgcacgcgcc gcgcggctgc tgggtatccg cgcactggtg gtcatgccca
     1201 acaacgcgcc cgcggtcaaa gtggcagggg ttcgtgccga tggcgcggag atcgagttcg
     1261 tcgggccgca caacgaggag cgcgtcgcgc gggcgcacca gatcgccgag cgcgacggca
     1321 tggtgctcgt cccgtcagcg aatcacccga gcgtgatcgc gggccagggc acgatcggcc
     1381 tggagatcgt ggagcagctg ctcgagatgt cgtggagcca tttgcccacc gcaagcgagg
     1441 cgccggtcgt tcttgtgccg gtcgggctgg gcggccttgc ggctggcgtc gcgaccgcga
     1501 tcaagagcgc gccagcatgg gccggcgcca ctggtcgtgt ctacggcgtc gaacctgagg
     1561 tcgcggccga cacccgcgac tcgctcgcgg cgcgcgagct aacgccgtgg ccgtctgcaa
     1621 gaacttcgtc gaccatcgcc gacgggctgc gcggcgaatc cccgtcgccc atcccgttcc
     1681 gccatctgct cgcccacctg gacggcgtcg taaccgtcag cgaggcggag atcggagcgg
     1741 ccgtcgccat cgcggcgcgc gaggcgcgtc tggtgctcga gccatcgggc gccgtttcgc
     1801 tggcggcgct gctcaaccgc cgcgccgagc ttccggaggg acccgtcgtg tgcgtcctct
     1861 ccggcggcaa cgtcgacccg gcgcgttacc tcgagttcct gcgcgtctga cgccgcgcgg
     1921 cttacgccgg gcgacttacg cgccctatca tcagccggat ggatcgcagc aaccctggct
     1981 tctcattgct cgaagcggca cgcatcgtcg cccgcggccc ggagctcgac accaagctcg
     2041 acgctctcgc cgggcaggtg ctgagtgcgg ccggtgccac cgccgccgtg atctacctgc
     2101 tggatccggt cgcgaacgtc ctcgtgccgg cggcccagtc cggattgcgc ggcaagatga
     2161 tcacggcgcg cgccgaggtc cccctggacg atccggaaga gctcgtcgcc aaggtagcgc
     2221 gcgagcggcg ccagatgagc atcagcggca gggaggcggc gagcgccttc acggggcagc
     2281 atcccgagtg gcacgacctt gtggcggtcc ctctggtggc ggccgacgaa acgggcgggg
     2341 aggatgctga gggcgtcctg ctggctgcct acgaagccgc gccgcccgag ggctccgacg
     2401 acctgctgac cgcgttggcc gacctttcgg ccattgccat ccgccaggca cggttgcagc
     2461 gcgcgctgct ggaacgggcc gactggatcg gccggctggc cagtaccgac ccgttgaccg
     2521 gactcgcgaa cagggtcacg ttcgagcgca tgctcgagct ggagctggca cgcgcgacgc
     2581 gccaggaatc gcagctgagc gtgctgctgt tcgatgtcga tggcttcgcc cagatcaact
     2641 ccgacgccgg gtcgccggcg ggcgatgagg tgttgcgcca cgtcgcggcg acgctcgccg
     2701 accaggtgcg cctcgtcgac acggtggccc gctacggcgg cgatgaattc gcgctgatcg
     2761 cccccggtgg tggcggcgaa gtggtcggcc ggcgggtgcg cgacgcactc ctcaagcgcg
     2821 aagcggcggg caagcccatc agcgtcagcg ttggcgcggt cgtctacccg attgacggcg
     2881 cgaccaccac cgagctcctg atagccgcgg ccaacgcgct ggctgaggcc aagcggcgcg
     2941 ggcgcggttc gatcttcgtc gcgagcgggt cctagaacag atcctggacc gcggcccaga
     3001 cacgggccgc gacttgatct cgatccgcgg cgccatcaac gacgcgccac cgctcaggat
     3061 cggctgctgc cagctggagg tagccatcgc gcacgcgctg gtggaaatcg gtgccgtgca
     3121 ggctggacgt ctcgaacctg ttcagacctt ccgccgggcc tgaatgccgg cgcgacaagc
     3181 cggcttccac gctgacgtcg atcagcaccg ttcggtgcgg catcagcccg ccggtcgcga
     3241 ggcgctggat cgcgcccagc tccgcgaggg gcgcgccacc gccgtagccc tggtacgcga
     3301 gcgtcgaatc ggcgtagcga tcgctgatca cgatgtcgcc gtgctcgagg gcgggacgga
     3361 tcacctcgat gacactgtgg tggcgcgcgg cgttgtagag cagcgcatca gagaccgcgg
     3421 tgtggccctc gccttcgcgg tcgagcagca cgttgcgcac gccttcaccg acgcgcgtcc
     3481 cgcccggctc gcgcgtcagg acgaccgtct tgcccagccc gcggacatgc tcggcgagca
     3541 gggtcgcctg gctgctcttg cccgcgccgt cagggccctc gatggtgatg aatagtctgg
     3601 tcaatgccgc cgaagtctag gactcggccg cgtttgacct cagctcgccc gctgcgatcg
     3661 ggtcgctggt tggcagctcc gagccgcccg tcggctcgac ggtcagggca atcacctgcg
     3721 tgcctgggcg gcccgaaagc gccatcaggg cgtagccatc gcggtcgacg ctgagcaggc
     3781 cggccgaagc cggcaccccg tcggcgatga accacgcctg gtacgtcttg ccggccggag
     3841 ccacgggcat gtcgatcagg acgatgtagc ccgccccatc tgcgccgaac gcggcgaagc
     3901 cgctcgcccc cgcagcggtc gcgctgcctt ccatgatcgc gaccgacgat ccgggtgccg
     3961 ccatcacgtc aatggcccgg gcaatcatct gcgcgcggtg ggcctcgagg gcggcattgg
     4021 actgcgcgac atacgcccac cccgccgtga ccgcgatcac caggaccgct gcggcagcgg
     4081 ccgcccagcc cagccaggac cgttgcatcg ggcgcgtggt tgccggtgca ggctcccaag
     4141 cgcggactcc ggcgccggcg cggtaatcgg ccatgacctt gttcttcaac gacgcgggcg
     4201 cgcccaccgg gtcggccagc gtcgccaggg cgggtgcgac cgccccgacc tctgcgaact
     4261 cgtcgtgcgc atccgggcag gacgcgagat gggcgtccac ctgcgcgcgc tcggccgggt
     4321 cgagcgcgtc gagcacgtac aggcccgcga gctcgatcgc ctcggcgtgg ttcatgtccc
     4381 atcctcttgg agagcgcgcc gcagcgcgag caatcccagc ctgacccgac tcttgacggt
     4441 accgaggggc gtgctcgtcc gctcggcgat ctcgacctgc gtcaggccct cgaaataggc
     4501 gagctggatt gcctcgcgct gggggtcggg caggctggcc atcgccgccg tcaccgaatc
     4561 acgatccagc gtgcgcgcca cctccggcca gacgtccggg gtggtcagcg cgggttccgc
     4621 ggtctcgatc tcgggcagcg cgctggtggg ccggcggcgg cgaacgatgt cgatggcacg
     4681 gtggtgggcg atcgacagca tccaggtgcg caccgacgag cggcccgcgg catagcgccc
     4741 cgcgttgcgc caaacaccga caaacgcctc ctggacagcg tcctgggcga gtgccgggtc
     4801 cctcgttatg cgcattgcca atccgtacat ctgggcctgg tagcggtcat acaagtcgga
     4861 cagcgcgcgc tcgtccgggc cggccgcgag acgctcgagg agcgcttcgt cggaaggccc
     4921 agatgcacgc ccgccgtcag cggcatccgt gtttggcggg ccgctcaccc gtatgtgcgt
     4981 cggtgagcgc cgattggttc acatccggtc agttcgcgca cgcgcgaagt ctagtcgggc
     5041 cgattcatgg gccgatttcg cccgccgatt catccctccg ccgccgcgct gcgtaaagcg
     5101 ggcagggatc ccggaattcc tgtccgttcc gagaggaggc cgctttacga gagcgttttt
     5161 ccgccgcgcg tcatccacgc aggcaagcac tggtcaagtc agttccacga ggaggtattt
     5221 cctgatcatg cgcaactcgc gccagcgcgc tatggcggct tcgatcgcca gcgctctgct
     5281 aatcctgacg ctcggcctta ccccggtcgt cagcggtgcg gatcaccttg atgcgcccgc
     5341 tgtcgcagcc cagggctcgg tcgacatcac cgacatctac acgttctccg ccaacaatgg
     5401 caactcgacc gtcttcatcg tcggcgtcaa cccgggcgca ggcgctctgc ccaactcggg
     5461 cgagaccttc ggaccgggtg tcgactacaa gatcaaggtc gacaccaacg gcgacctcaa
     5521 gcctgacgtc acgtaccgct atcgcttcgg cacaccgaat gggatgggcg tccagaccct
     5581 caagctgtgg cgcaacgggt cgatgctcgt caacggctgg accgatcaga acaaccttct
     5641 gcccggtggc ggtcgcacga cggcgggcct gttcgacgat ccattcttct tcgatctcgc
     5701 ggcgttccag ggcgcagtcc tcatgtccgg caacgggcgc accttctgcg acggtggaac
     5761 gaccgacttc ttcctcggcc tgaacatcag cgcgatcgtt ctgcgcgtgc ccaacagctc
     5821 gctgggcggc aacggcacga acatcggcgt ctgggcgacg accgaggccg tgcgcggtgg
     5881 caagcgcgtc cagctggacc agatgggccg gcccgcgatc aacaccgtgt tcaacaacca
     5941 gacggccgaa cacttcgatc gggacggatt caaccgagtc cagcccagcg agatggtcgc
     6001 agccggctat cgcgatcacg ctgaggatgt gctgacggca cttggtgccg ccgacccggc
     6061 cggtctggcc gcggtgctca tccccgacgt gctgaccttc cagaccggca acatggccgg
     6121 cttcctcaac ggccgccggt tggcgaatga cgtgatcgat gccgagctcg cgctcgtcac
     6181 caacaacggc atcaccaccg actgcattgg caacgacagc gccttcccgg gcgcctggcc
     6241 gtacctggca ccggccaact aggaaagtga acgccttcgc ggcgttgact cggagctcgg
     6301 gggccgccaa tgggcggtcc ccgaacttcc gggcgcgcct tggccgctcc ctcttctccc
     6361 gccgcgcggc gcttctggtg ccgccgctca tggcgctcgt cgtcgtgttc gcctttcaga
     6421 tgtggccacg accggtcgcg gcaccgccga ccaatgtcac tcccatcacc gagggcgccg
     6481 acacgttcac tgacgtccgc ccgccgggcg aggtgatcgc gctgtccggc gtggacacga
     6541 aagacaccga ccgcaagatc gcgttctggc aggcgcgcat cgccgccaac gcgcgcgacg
     6601 acatctcctg gacctacctg ggcgacctgt tcgagatcaa gggccggcag accggcgaag
     6661 tcagcaacta cctcagcgcc cgcgacgcgt acgcgaccgc gctcgacatc gcgcccggct
     6721 cggccgcggc agcgatcggc gcagcgcgga tctactcgac gctgcacgac tttctcggtg
     6781 cgcaggcggc cgcgaccgcc gtcctcgaag cggacccgta cgcgacgggc gctctcgcca
     6841 tcctgttcga cgcctcgctc gagatgggcc agctcgatat tgcgcaggac gcgctgacgc
     6901 tgctcttgga gcgcacccag acgcccgcgg tggtcggtcg gcaggcgcga ctcgccttcg
     6961 tgcgcggcga caccgccggc gcactcgcgc tgtccaacca agcagtgctc gacgcgacca
     7021 acgccggcga cccggtgtcg agcgtcgcct tctatcaata cgcccacgcg gagtacgcgc
     7081 tgctcgccgg cgatctcgat ggggcacaag ctggttacga ggcatcgctc gcggcgctgc
     7141 ccggctatcc gctcgcgctc ttcggccaag gtcgcgtggc gtacgcgcgc ggcgacgtgg
     7201 cccacgccat cgctctgctc gaggccgcga ctgccgccct gccacggccc gacatggtcg
     7261 cgttcctggg cgacctgtac gcgatcggcg gacggcccga agatgcggcg acccagtacg
     7321 acacggtcga gttcatccgc ggcctggcgg ctgccggcag cgccaaggtg tacgaccgcg
     7381 agtacgtcgg ctacctggcc gatcacgcac gcgacgccgc gctggccgtg gacttggcgg
     7441 ccgcggagct ggccgcgcgc aaggacgtgt acggctacga cgcgctcgcg tgggcgctcc
     7501 attccgcggg caaagacgcg gaagccctgg acaacgcgcg gctggccctc gccagcggca
     7561 cgcaggacgc gcgcctgctc atccacgccg ggctcatcga gctcgccaac ggcctcgatg
     7621 cggaggggca ggcgcacctt cgcgcggggc tggccctcaa cccttcgttc tcgccgctcg
     7681 tcgtcgaaat ggcgcgcgcc caggtggcac cgtgatgcgc cgcgtcgtcg cttcgctcgc
     7741 tctctccctc gcggccctgg cactcgtcgt cccggcgacg agtgcccacc cgctgggcaa
     7801 cttcacgatc aaccactaca acggcatccg ggtcgcgatc gaatcggtcg tgatcgacca
     7861 cgtcaccgat ttcgcggaga ttcccacgtt caccgagcgc caggcgatgg acgcgaacgg
     7921 tgacggcgcg ataagcgctg cagaatcgac cgactaccag ctggccgcct gcgacggcct
     7981 ggcctccacg ctcgccctga gcgccaacgg cgcacggctg gcactcacgc ccatcgccac
     8041 cggactgcat ttctcgatgg gttcggg
//
LOCUS       DATBRX010000108         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_145513, whole genome shotgun sequence.
ACCESSION   DATBRX010000108 DATBRX010000000
VERSION     DATBRX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145513"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VIF08_03945"
     CDS             complement(<1..369)
                     /locus_tag="VIF08_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8135168.1"
                     /translation="MLDLGWMADHLDELAFRTGQHILLTAIAVGAGLVISFGLAVLAV
                     RRRATYGPILAATDILYTIPSLALFVVLVSVTGISLLTVEIPLVLYTLVIFVRNIAAG
                     FDSVPRDVLEAANGLGFTKAQ"
     gene            complement(369..1331)
                     /locus_tag="VIF08_03950"
     CDS             complement(369..1331)
                     /locus_tag="VIF08_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135169.1"
                     /translation="MTAVDLEFRHVSRRYGETVAVDDLSLTVPAGKICVLVGPSGCGK
                     TTSLKMANRLIEPTSGQILIGGRDVMAQDPIELRRGIGYVIQQVGLLPHLTVGENVAI
                     VPRLLGWDKARITARVDELLALTGLAPKTYRDRYPAQLSGGERQRVGVARALAADPPL
                     MLMDEPFAAVDPIVRERLQNELLRLQEQLAKTIVFVTHDIDEAIKLGDLVAVLQVGGV
                     LAQFGPPDEILANPASEFVARFVGADRGLKRLSLTRVRDLELEPAPADYTAQPGQPTL
                     APQTTLKDALSMMLDSDVSVGVVVDRNGRVLGLLTYDKIAAALR"
     gene            1356..1901
                     /locus_tag="VIF08_03955"
     CDS             1356..1901
                     /locus_tag="VIF08_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY8135170.1"
                     /translation="MTANTAVPGGESAIIVPIAVPVTIARLRERMDPSAAVGVPAHVT
                     LLYPFMPVEGLDDDVRRAITRIVAAEPTFTVNFREVSRFPNVVYLPPDPPDPFRRLTA
                     ALAAEFPDYPPYEGAYETVIPHMTVAQDVSDDYYAAAEHALPAALPVRHIVREAWLIG
                     HLPEQPWHTLWRLPLGEAPRS"
BASE COUNT          341 a          725 c          698 g          326 t
ORIGIN      
        1 ctgggccttc gtgaagccga ggccgttggc ggcctcgagg acgtcgcgcg ggaccgaatc
       61 gaagcccgcg gcgatgttgc gcacgaagat cacgagcgtg tacaggacaa ggggaatctc
      121 gacggtgagc aggctgatcc cggtgaccga caccagcact acgaacaggg cgaggctggg
      181 gatcgtgtac aggatgtcgg tcgcggccag gatcggtccg tatgtcgcgc gcctgcgcac
      241 ggcgagaacc gccaggccaa acgagatcac aagcccagcg ccgaccgcga tcgcggtcag
      301 gaggatgtgc tggccagtgc gaaaagccag ttcgtcgagg tgatcggcca tccagccgag
      361 gtcgagcatc agcgcagcgc cgcggcgatc ttgtcgtacg tgaggagccc aagtacgcgg
      421 ccgttccgat cgaccacgac gccgaccgac acgtccgagt cgagcatcat cgacagcgcg
      481 tccttgagcg ttgtctgcgg cgccagggtg ggttgcccgg gttgcgcggt gtagtcggcc
      541 ggagctggct ccagctccag gtcgcggacg cgcgtcaacg acaggcgctt gagcccgcgg
      601 tcggcgccga cgaaccgtgc gacgaactcg gacgctgggt tggccaggat ctcgtcaggt
      661 ggcccgaatt gcgcgagcac cccgccaacc tggagcaccg cgaccaggtc gcccagcttg
      721 atcgcctcgt cgatgtcgtg cgtcacgaag acgatcgtct tggcgagctg ctcctggagg
      781 cgcagcagct cgttctggag tcgctcgcgc acgatggggt cgaccgccgc gaacggctcg
      841 tccatgagca tcagcggtgg gtccgccgcg agcgcccggg cgacgccgac gcgctgacgc
      901 tcgccaccgg acagctgggc cggatatctg tcgcgatagg tcttcggggc aagtccggtc
      961 agcgcgagca gctcgtcgac ccgcgccgtg atgcgcgcct tgtcccagcc gagcagccgc
     1021 ggcacgatgg cgacgttctc gcccaccgtc aggtgcggca gcagcccaac ctgctggatg
     1081 acgtagccga tgccgcgccg cagctcgatc gggtcctgcg ccatcacgtc gcggccgcca
     1141 atcaggatct gcccggaggt cggctcgatg agccggttgg ccatcttgag gctggtcgtc
     1201 ttgccgcagc ccgacggacc gacgaggacg cagatcttgc ccgccggcac ggtgagcgac
     1261 aggtcgtcga ccgcaaccgt ctcgccatag cggcgcgaga cgtgacggaa ttcgaggtct
     1321 acggcggtca tgtgggcacc gtatacgctt gcccggtgac cgccaacaca gccgttcccg
     1381 gtggcgagag cgcgatcatc gtcccgattg ccgtaccggt gacgatcgct cgattgcgcg
     1441 agcggatgga cccgtcggct gcggtgggcg tgcccgcgca cgtaaccctg ctctatccgt
     1501 tcatgcccgt tgagggcctc gacgacgacg tgcgccgggc gatcacccgt atcgtcgccg
     1561 ccgagccgac gttcaccgtc aacttccgcg aagtcagccg ctttcccaat gtcgtctatc
     1621 tgccgcccga cccgcccgat cccttccgcc gattgaccgc cgcactggca gccgaattcc
     1681 ccgattaccc gccctacgag ggtgcctacg agacggtcat cccgcacatg acggtcgccc
     1741 aggacgtgtc cgacgactac tacgccgcgg ccgagcatgc gctgcccgcc gcgctgcccg
     1801 tgcgccacat cgtgcgcgag gcgtggctga tcggccatct cccggagcag ccgtggcata
     1861 cgctgtggcg cctgcctctc ggcgaagcgc cccggtccta gccgcgttta gactcgcgcc
     1921 atgccacaga tccaccttcg cgccgagccc ggtgactacg cgcccctcgt cctgatgccg
     1981 ggcgatcccg gccgggcgac gatgatggcc ggcctgctcg atggcggcat ggacaaagtg
     2041 aaactcgtca accagcaccg ctcgctgttc ggctacaccg ggacctacaa
//
LOCUS       DATBRX010000109         2666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1486225, whole genome shotgun sequence.
ACCESSION   DATBRX010000109 DATBRX010000000
VERSION     DATBRX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486225"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VIF08_03960"
     CDS             complement(<1..980)
                     /locus_tag="VIF08_03960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8135171.1"
                     /translation="MAETFDVVVIGAGVHGASTAFHLAERGAKVVVLERTAAASGATG
                     RSSGLVRMHYDLKAESALAWRSYEYFINWETRVGGECGFTRTGFVRIVPPAHESNLRA
                     NVADQQAIGIRTSIIPAADVKALAPMLTTDDFDVAAYEPDSGYADPTSTCNSLLAAAR
                     RLGATYRHSTPVESIELDGDRVVGVRTASGSISAPIVVLAAGGWSGPLAKSAGVDIDL
                     KVWHHDTGFLRRPMGDAAGLPVVIDDINELYFRPEGQGLALIGLEDGNRIDDRPGDDT
                     RSDPEFVEHMVDRLTRRVPDYAHAGFQGAHGGTDGITPDQRMIVGAYGPDG"
     gene            1017..1613
                     /locus_tag="VIF08_03965"
     CDS             1017..1613
                     /locus_tag="VIF08_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135172.1"
                     /translation="MLRRAIAAALCLVVLSTSTTFASTTTVYVVNYAYQPAKPKIALS
                     NGILWRNTSDTSHTVTFNVPAVAPGSIGSLGPGGAGSLTFFHGGAYAYHCIFHPQQVG
                     TIKVKPSISPTSGTTSTNFSLRVATQNAPSGFVQDVQRRKGSGSWSLWQSTNLQLVVF
                     NPAPSENGTWHFRTRYRKTSTGEMTGWSPELAVVVSKP"
     gene            complement(1785..2246)
                     /locus_tag="VIF08_03970"
     CDS             complement(1785..2246)
                     /locus_tag="VIF08_03970"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135173.1"
                     /translation="MDGAPTHVDWTRGDYEISTDPARLDLDLTHRFLSEDAYWSPGVP
                     REIVERAIGNSMVFGVYHVGPSGSAQVGLARVVSDRATFAWICDVFVLPEHRGQGLGK
                     WLMECITSHQELQGLRRMVLATRDAHGLYAQYGFTPLEDPSRFMVRRDPGR"
     gene            complement(2254..>2666)
                     /locus_tag="VIF08_03975"
     CDS             complement(2254..>2666)
                     /locus_tag="VIF08_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8135174.1"
                     /translation="APADRSERGVRGRAPLTLAALGAAGLLVAALAACGGAPAPLPTP
                     PPGAVIITAQGTAFVSPPYTAPANAAFTLFFENRDNEPHNVRVWDAANASVYTTEIIQ
                     GPAAKAEQVPALPAGTYRLTCDIHPDMTAQLNVQ"
BASE COUNT          434 a          909 c          901 g          422 t
ORIGIN      
        1 ccgtcgggcc cgtacgcgcc gacgatcatg cgctggtcgg gcgtgatgcc gtccgtccca
       61 ccatgcgcgc cctggaagcc ggcatgggcg taatcgggca cccggcgggt caatcgatcg
      121 accatgtgct caacgaactc ggggtcgctg cgcgtgtcgt cgcccggccg atcgtcgatc
      181 cggttgccgt cctccaggcc gatgagagcc aggccctggc cttcgggtcg gaagtagagc
      241 tcgttgatgt catcgatgac gacgggcagg ccagcggcgt cacccatcgg ccgcctcaag
      301 aaacccgtgt cgtgatgcca gaccttgaga tcgatatcga cccctgccga tttggccaac
      361 ggacccgacc acccgcccgc agcgagcacg acaatgggtg cgctgatgct gccgctcgcg
      421 gttcggacac cgacgacacg gtcgccgtcg agttcgatcg actccaccgg cgtcgaatgg
      481 cgataggtcg cgccgagtcg ccgcgccgcg gccaacaacg agttgcacgt ggaggtggga
      541 tcggcgtagc ccgagtcggg ctcgtatgcg gcgacatcga aatcgtcggt cgtcagcatt
      601 ggggcgagcg ccttcacgtc cgcggcgggg atgatcgatg tgcggatgcc gattgcctgt
      661 tgatcggcca cgttggcgcg caggttgctc tcgtgcgctg gcggcacgat gcgcacgaag
      721 cccgtcctgg tgaagccgca ctcgccaccg acgcgcgtct cccagttgat gaagtactcg
      781 tacgagcgcc aggcgagcgc gctctccgct ttgaggtcgt agtgcatccg caccaggccg
      841 ctcgaccgcc ccgtcgcacc cgacgcggcg gcggtccgct ccagcaccac caccttggcg
      901 ccgcgttccg cgagatggaa cgccgtgctc gcgccatgca cgcccgcgcc gatcacgaca
      961 acgtcgaatg tctcagccat cacccggagt ctagacagcg gccccacaat gtccgggtgc
     1021 ttagacgcgc catagccgcc gccctgtgcc tggtcgtcct gtccacctcg acgactttcg
     1081 catcgaccac gacggtctac gtcgtcaact acgcctacca gcccgcgaaa cccaagatcg
     1141 cgttgagcaa cggcatcctg tggcgcaaca cgtcggacac cagccacacg gttacgttca
     1201 acgtccccgc ggtcgcgccg ggatcgatcg gcagcctggg ccccggcggt gccgggtcgc
     1261 tgacgttctt ccatggcggc gcgtacgcct accactgcat ctttcacccg cagcaggtcg
     1321 gcacgatcaa ggtcaagccg agcatctcgc cgacctcggg cacgacgtcg accaacttca
     1381 gtctgcgcgt cgcgacgcag aacgcgccgt cgggcttcgt ccaggacgtg cagcgccgca
     1441 agggcagcgg ctcatggtcg ctctggcaga gcaccaactt gcagctcgtg gtgttcaacc
     1501 cggcaccgag cgagaacggc acgtggcact tccggacgcg ctaccgcaag acgagcactg
     1561 gcgagatgac cggttggagc cccgagctgg cggtcgtcgt cagtaagccc tagcgacccg
     1621 cctgctggtc ggccgagcgc gttcgattgg ccgcgctcgt tcgatcggcc gcgctcggcg
     1681 agatggagtg gcgcgggaaa acgacctgct ggtggtgagt cagagatgga agaggccttg
     1741 gaaatgggta tctgctggga gccagcgggt gggtggcggc ccggctacct gccggggtcc
     1801 cggcgcacca tgaagcgcga ggggtcctcg agcggcgtga agccgtactg cgcgtagaga
     1861 ccgtgggcgt cacgcgtcgc cagcaccatc cgcctcaggc cctggagttc ctgatgcgac
     1921 gtgatgcatt ccatgagcca cttgccgagc ccttggccgc gatgctcagg tagcacgaac
     1981 acatcgcaga tccaggcgaa tgtcgccctg tccgacacga cccgggcgag gccgacctgg
     2041 gcgctgccgc tgggcccaac gtggtagacg ccgaagacca tcgagttgcc gatcgcccgc
     2101 tcgacgatct cgcgcgggac gccgggcgac cagtaggcgt cctcggacag gaaacgatgc
     2161 gtcaggtcga gatcgaggcg cgccgggtcg gtcgagatct cgtagtcgcc acgcgtccag
     2221 tcgacgtgcg tcggcgcgcc gtccatggtg cggctactgg acgttgagct gggcggtcat
     2281 gtccggatga atgtcgcacg tcaagcggta cgtgcccgct ggcagcgccg ggacctgctc
     2341 ggccttggcc gcggggccct ggatgatctc tgtcgtataa acgctcgcgt tggccgcgtc
     2401 ccagacgcgc acgttgtgcg gctcgttgtc gcggttttcg aagaagagcg tgaaggccgc
     2461 gttcgccggc gccgtgtacg gcggtgacac gaacgccgtg ccctgggcgg tgatgatcac
     2521 tgccccagga ggcggcgtcg gcaacggcgc cggcgcgccg ccgcacgcag ctagggccgc
     2581 gaccaacaat ccggccgcgc cgagcgcggc aagagtgagt ggagcgcgac ccctcacgcc
     2641 gcgctccgac cggtcagccg gcgcag
//
LOCUS       DATBRX010000110         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3050593, whole genome shotgun sequence.
ACCESSION   DATBRX010000110 DATBRX010000000
VERSION     DATBRX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050593"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..937)
                     /locus_tag="VIF08_03980"
     CDS             complement(2..937)
                     /locus_tag="VIF08_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8135175.1"
                     /translation="MSVQATDLVTGAFGYTGSRIAERLLARGRAVRTLTRRTAVDHPL
                     ATRVERFPSAFDDANLDGALAGVDTLYVTYWMRFPRGGATWDELVANVARLAGAARRQ
                     GIRRLVYISVSNARPDSTTAYFRAKAAAEGRIRAAGTGAMSVAIVRPTLLYGPSDILI
                     NNMAWSLRRLPVFGIPGDGRYRVQPVHVDDVADLCIRLGSGDDHVETDAAGPETLTFD
                     ELVRLVKTAVRSRALLAHMPVAVVLATTRLMGLLVRDVVLTRDEIRELAESLLTSSAN
                     PAGTTSTRFSEWLAANAADVGRCYSSELGRNFRLR"
     gene            957..1901
                     /gene="cofD"
                     /locus_tag="VIF08_03985"
     CDS             957..1901
                     /gene="cofD"
                     /locus_tag="VIF08_03985"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEY8135176.1"
                     /translation="MKVLVLAGGFGGARMAHGFELLGDQVSLSVIVNTADDLELHGLL
                     VSPDLDTIMYTLAGLANDATGWGVLDETWSGAEMLQRYGAPTWFKLGDRDLATHLLRT
                     AAVRGGERLTDVEATLASTLGVRAKLLPMSDSPIRTSVRTADGWLDFQEYFVRRHHAD
                     EALELRFSGAADARPTPEVIQAIGGADLIVIAPSNPFVSVGPILAVPGLLARLLEAPA
                     PIVAVSPIVGGAAVRGPAADMLVSLAHRPATAAGLATYYAATYPGLIDVLVLDNTDAG
                     HEEAVARTGMRALVTNTLIASPDARRRLAEELLALGRQ"
     gene            1922..2611
                     /gene="cofC"
                     /locus_tag="VIF08_03990"
     CDS             1922..2611
                     /gene="cofC"
                     /locus_tag="VIF08_03990"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEY8135177.1"
                     /translation="MIGDLSRVHVVVPLRGADSGKSRLGQALDAEERGALVLGLLART
                     LDVLATWPQAQRVYLVTGDAATGELARRAQPALTVLTEPRQGGLNAALREARDAAVDL
                     GATAVLMLPADLPLLDTAALDRLLDGADAALAAGQGHALVVVAPADARGGTNALLVSP
                     PTLIDPQFGEASLEAHLRAAALADATVQLVHDPALAFDLDTPDDLERLDTATVLDLEH
                     RGQALLAMQVA"
     gene            2611..3402
                     /gene="cofE"
                     /locus_tag="VIF08_03995"
     CDS             2611..3402
                     /gene="cofE"
                     /locus_tag="VIF08_03995"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY8135178.1"
                     /translation="MPTITVVPLPPLPEVQPGDDLPALIAQAWWELAAAQNDLAPRRT
                     DVLVVTQKIVSKAEGKLVDLTTITPRQEAIDFAKQWDRDARQVEVVLRESKEVLKFER
                     GIVVSRTHHGFVCANAGVDASNTGQKDIVTLLPDDPDQSARDIRDRLPALLDLPSDQA
                     PAVIISDSFGRPWRFGIMDVALGVAGIEPLIDLRDTPDADGRIMHSTIVAIADEIASA
                     AELAAGKSSNQPVVLVRGVEFYNRGQSEPSVVREVVMPTEMDLFK"
     gene            3417..4349
                     /locus_tag="VIF08_04000"
     CDS             3417..4349
                     /locus_tag="VIF08_04000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY8135179.1"
                     /translation="MKIGLQISSFSWPGGDAQIGPTLARLCRAADDAGFDSIWVMDHL
                     FQIRSVGEIEEPMLEGTTALGFMAAHTQRARLGLMVGAVPYRHPGVWIKSTTTLDVLS
                     EGRAWFGIGAAWNVEEARALGIPFPDLTDRFRALEDTLKMAHQAWTGAGEPGSAFTGK
                     TVRATRLMNSPQVLSRPRVPILVGGGGERTTLRLVARYADACNVFGAPDMLRHKFSVL
                     AEHCADVGRDYNTIERTNLSSVSITPDGRQGSLTPSGLVERLGNWAEAGTHHTIFSVR
                     NVWDLSKLELIGRDVIPQVRALGDPSPLDLTKVG"
     gene            4391..>5142
                     /locus_tag="VIF08_04005"
     CDS             4391..>5142
                     /locus_tag="VIF08_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8135180.1"
                     /translation="MDSTKLGFPSISIVDDGAPADATSERLISQQPSGNGASPDGDGN
                     NHAKVVIVGSGPAGLTAAIYAARANLAPIVIGGYAPGGQLMITSDVENYPGFPEGVQG
                     PELMQKFREQAERFGTRFVDVDVEKVDFSQRPFRLWAEGKQYTGDSVIVATGASALWL
                     GLENETRLRGRGVSACATCDGFFFRGKKVAVVGGGDTALEEATFLTRFATEVVMLHRR
                     DKFRGSKIMQDRALENPKIKVQWNTEVADVLGA"
BASE COUNT          814 a         1789 c         1764 g          775 t
ORIGIN      
        1 gtcagcggag gcggaagttg cggccgagct cggacgagta gcagcggccg acatcagccg
       61 cattggccgc cagccactcg ctgaagcgcg tcgaggtcgt gccggcgggg ttcgcggagc
      121 tggtcagcag gctttccgcg agctcgcgga tctcgtcgcg cgtcaggacc acgtcgcgca
      181 ccagcaagcc catcaggcgc gtcgttgcga ggacgaccgc gacgggcatg tgcgccagca
      241 gcgcccggct gcgcaccgcg gtcttcacca accgaactaa ctcgtcgaac gtgagcgtct
      301 cgggcccagc ggcgtcggtc tcgacgtggt catcgccgct ccccagccgg atgcacagat
      361 cggcaacgtc gtcgacgtgc accggctgga cgcggtagcg gccgtcgccg ggaatgccaa
      421 acacgggcag gcggcgcagc gaccaggcca tgttgttgat gaggatgtcg gatgggccgt
      481 agagcaacgt gggccgcacg atcgcaacag acatcgcgcc agtgccggca gcccggatgc
      541 gaccttcggc ggccgccttg gcgcggaagt aggccgtggt cgagtcgggc ctggcgttgc
      601 tgacgctgat atagaccaga cgccggatgc cctggcgccg cgcggcaccg gcgagacgcg
      661 cgacgttggc gaccagctcg tcccaggtcg cgccgccgcg cgggaagcgc atccagtagg
      721 tgacgtacag cgtatcgaca ccggcgagcg cgccatccag gttcgcgtcg tcgaatgccg
      781 aggggaacct ctcgacgcgc gtcgcgagcg gatggtcgac ggccgtccgc cgcgtcaaag
      841 tgcgcaccgc tcggccgcgc gcgagcagcc gctcggcaat ccggctgccg gtgtagccaa
      901 aggcgccggt cacaaggtcg gtggcttgaa cgctcacggg acatatagtc ggtgagatga
      961 aggtccttgt tctggccggt gggttcggcg gcgcgcgcat ggcgcacggg ttcgagctgc
     1021 tcggcgatca ggtctcgctg agcgtcatcg tcaacaccgc ggacgacctc gagctgcacg
     1081 ggctgctcgt gtcgcccgat ctggacacca tcatgtacac gctggccggc ctggccaacg
     1141 acgccacggg ctggggggta ctcgacgaga cctggtccgg ggcggagatg ctgcagcgct
     1201 acggggcgcc gacctggttc aagctgggcg atcgcgacct ggcgacgcac ctgctgcgca
     1261 ctgcggccgt acgcggcggg gagcgcctga ccgacgtcga ggcgactctt gcgagcacgc
     1321 tgggcgtgcg cgccaagctg ctgccgatgt ccgattcacc gattcggaca tccgtccgca
     1381 cagccgacgg ctggctcgat ttccaggagt acttcgtgcg ccgtcaccac gccgacgagg
     1441 cactcgagct gcgcttttcc ggagcggccg acgcccgacc cacgccggag gtgatccagg
     1501 ccatcggtgg tgccgacctc atcgtgatcg cgccgtccaa tccgttcgtc agcgttggcc
     1561 caatcctggc cgtgcccggt ctgcttgcgc gtctgctcga agccccggcg ccgatcgtcg
     1621 cggtcagccc catcgtgggc ggcgccgcgg tgcgcggacc ggcggcggac atgctcgtgt
     1681 cgctggccca ccgaccagcg accgccgccg ggctggcgac ctattacgcc gccacgtacc
     1741 cgggcctgat tgacgtgctc gtgttggaca acaccgacgc tggacacgaa gaagcggtcg
     1801 cacgcaccgg catgcgagcg ctcgtcacga acacgttgat cgcctcgccg gacgcgcgtc
     1861 gccgcctcgc cgaggagctg ctcgcgctcg gacggcaata ggacgcggct accatcgcgc
     1921 cgtgattggc gacctgtccc gcgttcacgt ggtcgtgccg ctgcgcggag ccgactccgg
     1981 caagagccgg ctgggccagg cgctcgatgc ggaagagcgc ggcgccctcg tcctggggct
     2041 gctggcgcgg acgctcgacg tcctcgcgac gtggccgcag gctcagcgcg tctatctcgt
     2101 cacgggcgac gccgccaccg gggagctggc gcggcgcgca caaccggcgc tgacagtcct
     2161 gaccgagccg cgccagggcg ggctgaacgc ggctttacgt gaggcccggg acgcggccgt
     2221 cgatctgggc gcgaccgcgg tgctgatgct gcccgccgac ctgccgctgc tcgacaccgc
     2281 ggcgctcgat cggctgctcg acggtgccga cgccgcactc gccgccggcc agggccacgc
     2341 cctggtcgtc gtcgctcctg ccgatgcgcg cggcggcaca aacgccctgc tcgtcagccc
     2401 gcccacgctc atcgaccccc agttcggcga ggccagcctc gaggcgcacc tgcgcgccgc
     2461 ggccctggcc gacgcgaccg tccagctggt gcacgatccc gcgctcgcct tcgacctgga
     2521 tacgccggac gacctagagc ggctcgacac cgcaacggtc ctcgacctgg agcatcgcgg
     2581 ccaggcgctg ttggccatgc aggtcgcctg atgccgacca tcaccgtcgt ccccttgccg
     2641 ccgctgcccg aggtccagcc gggtgacgat ctgcccgcgc tgatcgccca ggcctggtgg
     2701 gagctggccg cggcgcagaa cgacctcgcg ccgcgccgca cagacgtcct cgtcgtcacc
     2761 cagaagatcg tctcgaaggc agaaggcaag ctggtcgacc tgaccaccat caccccaaga
     2821 caggaagcga tcgacttcgc caagcaatgg gatcgcgacg cgcgccaggt ggaagtggtg
     2881 ctcagggaat cgaaggaagt cctgaagttc gaacgcggca tcgtcgtcag ccgcacgcat
     2941 cacgggttcg tgtgcgccaa cgccggcgtc gatgcctcga acacgggcca gaaggacatc
     3001 gtgacgctgc tgcctgatga ccccgatcag tcggcccgcg acattcgcga ccgcctccct
     3061 gccctgctgg acctgccctc cgaccaggcg ccggcggtca tcatcagcga ctcgttcggc
     3121 cgcccgtggc gcttcggcat catggatgtc gcgctgggcg tggccgggat cgagccgctg
     3181 atcgatctgc gcgacacgcc cgacgccgac ggccggatca tgcattccac gatcgtcgcc
     3241 atcgccgatg agatcgccag cgccgccgag ctcgccgcag gcaaatcgag caaccagccg
     3301 gtcgtgctgg tgcggggggt cgagttctat aaccgcggtc agtcggagcc gtcagttgtg
     3361 cgcgaggtgg tcatgcccac ggagatggac ctcttcaagt agcctcggca tccgtcatga
     3421 agatcggcct ccagatctcc tccttttcct ggccgggcgg tgacgcccag atcggcccaa
     3481 ccctggcgcg cctctgtcgc gcagccgacg acgccggctt cgactcgatc tgggtcatgg
     3541 accacctatt ccagatccgc agcgtgggcg agatcgagga gccgatgctg gagggcacga
     3601 ccgccctcgg tttcatggcc gcccacacgc agcgggctcg gctggggctg atggtcggtg
     3661 ccgtccccta ccgtcacccg ggcgtctgga tcaaatcgac gacgaccctc gacgtgctga
     3721 gcgagggccg cgcctggttc ggcatcggtg cggcgtggaa cgtggaggag gcgcgcgccc
     3781 tgggaattcc ctttcccgat ttgaccgacc gattccgagc gctggaggac accctcaaga
     3841 tggcccacca ggcgtggacg ggcgcgggcg agccaggcag cgcattcacg ggcaagaccg
     3901 ttcgcgccac ccggctgatg aactcgccgc aggtcctgtc gcggccgcgc gtccccatcc
     3961 tggtcggcgg cggcggtgag cgaaccacgc tccgcctggt cgcgcgctac gcggacgcgt
     4021 gcaacgtgtt tggtgcgccg gacatgctgc gccacaagtt ctctgttctg gccgagcact
     4081 gcgccgacgt aggccgcgac tacaacacca tcgagcgcac aaacctttcg agcgtgtcga
     4141 tcaccccgga cggccggcag ggctcactca cgccgagcgg cctggtcgag cggctgggca
     4201 actgggccga ggcaggcacg caccacacga tcttctccgt gcgcaacgtg tgggacctgt
     4261 ccaagctcga gctcatcggc cgcgacgtta tcccgcaggt acgcgccctg ggcgacccgt
     4321 caccgctcga cctgaccaag gtcggataga tactcccggg gagtatgcag ccctgctaaa
     4381 gttgagggag atggacagca ccaagctcgg ctttccgagc atctcgatcg tcgacgacgg
     4441 cgcgccggcc gacgccacga gtgagcgtct catcagccag cagcccagcg gcaacggcgc
     4501 ctcccctgac ggcgacggta acaatcatgc caaagtcgtc atagtcggct cgggcccggc
     4561 cggcctgacc gcggccatct acgccgcgcg ggccaatctg gcgcccatcg tcatcggcgg
     4621 ttacgcgccg ggcggccagc tgatgatcac gagcgatgtc gagaactacc ccggcttccc
     4681 ggagggtgtg caaggcccgg agctgatgca gaagttccgc gagcaggccg agcgcttcgg
     4741 cacgcgcttt gtcgatgtcg atgttgagaa ggtcgacttc agccagcgtc cgttccggct
     4801 gtgggcggaa ggcaagcagt acacaggtga ttcggtgatc gtggccaccg gcgcgtcagc
     4861 gttgtggctg ggccttgaga acgagacgcg gctgcgcggc cgcggggtca gcgcctgcgc
     4921 gacgtgcgac gggttcttct tccgcggcaa gaaggtcgcg gtcgtgggcg gcggcgacac
     4981 ggcgctcgaa gaggcgacct tcctcacgag attcgcgacc gaggtcgtga tgctccaccg
     5041 ccgcgacaag ttccgcggct caaagatcat gcaggatcgc gccctggaga atcccaagat
     5101 caaggtgcaa tggaacaccg aggtcgcgga cgtcctgggc gc
//
LOCUS       DATBRX010000111         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2268117, whole genome shotgun sequence.
ACCESSION   DATBRX010000111 DATBRX010000000
VERSION     DATBRX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268117"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VIF08_04010"
     CDS             complement(<1..1089)
                     /locus_tag="VIF08_04010"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY8135181.1"
                     /translation="MAARRIGILTGGGDVPGLNSVIKSVVYNATEIGCEVFGIRRGWE
                     GLTHMRRAEALSAGGRLDPDYIRPLDRVNTRTIDRSGGTMLHTSRTNPSRMPARRLPA
                     ALTPERAAQMQVSDDIYDLTPLVVDNIEYLGLDYLVTIGGDDTLSYSKVLDRVGVPLI
                     AIPKTMDNDVQGTEYCIGFSSAITRAKELITRQRTTLGSHERIGVFRIFGRDAGFSAL
                     YTAYVTSARCVIPEAPFDLARLIDVLVEDKRANQSRYSFVIASEGAIWQGGALAEVGE
                     TDSFGHRHKVDVGEALADEIKKRSGEEAVSSELTYDLRSGDPDSLDTMVAITFANIAV
                     DLIREGGITGRMIAVQDGKYTHVPIPEPG"
     gene            complement(1109..2005)
                     /gene="ccsB"
                     /locus_tag="VIF08_04015"
     CDS             complement(1109..2005)
                     /gene="ccsB"
                     /locus_tag="VIF08_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY8135182.1"
                     /translation="MTLAKLSYYAFDVGTVLAFIGFLLLIVHTTLLAAGRRQTFAAAG
                     AGGRTVTIGQSGPSATSAGSIGQAFIWASTILLAVGLIVRAYLVGRGPWGNLYEFSVA
                     FAFGVMAGYLYLGRRYPIRAIGFLPIGVAAFLLGYAFTLPQDISPLVPALDNPPLLTI
                     HVAMAMISYGIFAVSFGAAVGYLVQGRENRVSWLPSAKVLDEVAYRAVIIGFPIFATL
                     IVLGSWWAAIAWGRYWGWDPKETSALVTWLIYAVYLHARSQRGWAGRPAALILVIGFG
                     AVLFTYLGGNLFFGGLHSYSGL"
     gene            complement(2021..>2363)
                     /locus_tag="VIF08_04020"
     CDS             complement(2021..>2363)
                     /locus_tag="VIF08_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135183.1"
                     /translation="LYWLYVRAREKRGKVRFDDPQPTLLATNADGTYTYPPNAPYCPT
                     HHLVYPPTAHECDIDRGDLLVRCPVDDTVRPAGQQLCRSCGTRYQLGASLAPVVVRKR
                     GTPPAGGAAVA"
BASE COUNT          418 a          765 c          817 g          363 t
ORIGIN      
        1 gcccggctcc gggatgggca cgtgggtgta cttgccgtcc tgcaccgcga tcatgcggcc
       61 ggtgatgccg ccctcgcgga tgaggtcgac cgcgatgttg gcgaaggtga tcgcgaccat
      121 cgtgtccagt gaatcgggat cgccgctgcg caggtcatag gtcagctcgg acgagacggc
      181 ttcctcgccc gaccgcttct tgatctcgtc ggccagcgcc tcgccgacgt cgaccttatg
      241 gcggtgcccg aacgagtcgg tctcgccgac ctcggccaac gccccaccct gccagatcgc
      301 gccttccgaa gcgatgacga aggagtagcg cgattggttg gcgcgcttgt cctcgacgag
      361 cacgtcaatc agccgcgcga ggtcgaacgg cgcttccggg atgacgcagc gcgcggacgt
      421 gacatatgcg gtgtacagcg cggaaaagcc cgcgtcgcgg ccaaagatgc ggaacacgcc
      481 gatgcgctcg tgcgagccca gggtcgtgcg ctggcgcgtg atcagctcct tggcgcgggt
      541 gatcgccgac gagaagccga tgcagtactc ggtgccctgg acgtcgttgt ccatcgtctt
      601 ggggatcgcg atcagcggca ccccgacgcg gtcgagcacc ttggagtacg agagcgtgtc
      661 gtcgccacca atcgtcacga ggtagtcgag gcccaggtac tcgatgttgt caacgacgag
      721 cggcgtcagg tcatagatgt cgtcgctgac ctgcatctgc gccgcgcgct ccggcgtgag
      781 cgcggccggc agacgacgcg cgggcatccg cgagggattc gtacgactgg tgtggagcat
      841 ggtcccgccg gagcggtcga tggtccgcgt gttgaccctg tcgagcggcc ggatgtagtc
      901 cggatccagc cgcccgccgg cggacagtgc ctccgcccgg cgcatgtgcg tcaagccctc
      961 ccagccgcgc cggatgccga agacctcgca cccgatttcc gtcgcgttgt agacgacgct
     1021 cttgatgacc gagttgagtc ccgggacgtc gccgccgccg gtcaggatgc cgattcgtcg
     1081 tgctgccacg atttgcctcg attgggccct agagcccgct ataagagtgg agacccccaa
     1141 agaacagatt gccgccaagg tatgtgaaga gcaccgcccc aaagccgatc accagtatca
     1201 gcgccgcggg ccgaccagcc cagccgcgtt ggctgcgggc atggagatac acggcgtaga
     1261 tgagccacgt gacgagcgca ctggtttcct tgggatccca gccccagtag cggccccacg
     1321 cgatcgcggc ccaccatgag cccaggacga tcagcgtcgc gaagatcggg aagccgatga
     1381 tcaccgcgcg atacgcgacc tcgtcaagca ccttggcact cggcagccag ctgaccctgt
     1441 tctcgcggcc ctggaccaga taccccacgg ccgcgccgaa gctcaccgcg aagatgccgt
     1501 agctgatcat cgccatcgcg acgtggatcg tgaggagcgg cgggttgtcg agggccggca
     1561 caagcgggct gatgtcctgc ggcagcgtga acgcgtagcc cagcaggaaa gccgccacgc
     1621 caatcggcag gaacccgatg gcacgaatgg ggtaacgccg gcccaggtac aggtagcccg
     1681 ccatcacgcc gaaagcgaag gcgacgctga actcgtacag gttgccccac gggccgcggc
     1741 cgacgagata ggcgcgcacg atgagcccga ccgcgagcag gatcgttgac gcccagatga
     1801 aggcctggcc gatcgagccg gcggaggtgg ccgatggtcc ggactggccg atggtcacgg
     1861 tcctgccgcc tgctccggct gcggcgaacg tctgccgccg acccgcggcg agcagcgtcg
     1921 tatgcacgat cagcagcagg aagccgatga atgccagcac ggtgccgaca tcgaaggcgt
     1981 aataggagag tttcgccagt gtcatgaacc ccgcgactcc ctaagcgacc gcggcgccac
     2041 cggctggggg agtgccacgc ttgcgaacga ccactggagc cagcgacgcg ccgagctggt
     2101 agcgggttcc acatgagcgg cacagctgct ggccggccgg tcggacggtg tcgtcaacgg
     2161 ggcagcgcac cagcaggtcg ccgcgatcga tgtcgcattc gtgagcggtc ggcggataga
     2221 ccaggtggtg cgtcgggcag tagggcgcgt tgggcgggta ggtgtacgtg ccgtcggcgt
     2281 tggtcgcgag gagagtcggc tggggatcgt cgaagcgcac cttgccgcgc ttctcgcgtg
     2341 cgcgtacgta caaccaatac agg
//
LOCUS       DATBRX010000112         8793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2716145, whole genome shotgun sequence.
ACCESSION   DATBRX010000112 DATBRX010000000
VERSION     DATBRX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716145"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="VIF08_04025"
     CDS             <1..1793
                     /locus_tag="VIF08_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8135184.1"
                     /translation="GAYVAITLNGSVGDAAAALQTLGYDDLLTPAAHAAIKASATAPA
                     GDPLRDAIGIGLLVLVAGVAVSFIYSARDRLPVLGRRGSRFSAIMPATPRSAASGLPV
                     PGKVAAAPSGSSRPVHLSDVAGCDEAKLELAEPIEFLRHPDGFRSLGANVPRGILLYG
                     PPGTGKTMLARAVATEAGVPFLHASGSDFVEKYVGTGARRVRDLFGQAKRLGKAVIFI
                     DEFDALGKARGGANSHEEREQTLNQLLVELDGFGTSGEIVVIAATNRLDILDEALLRP
                     GRFSRKVHVGLPDVTGREAILAVHARGKPLAPEVDLEALARKTYGFSGAQLADLLNEA
                     AIMAARRGSATVTNDDMHAGWLKVAVGTGRRRSMNERDRAIIAAHEVGHAICGKVHGD
                     SRRVEEISLFAHGDALGVTVSSQEDNDLPAESDLRARLVALMGGRAAEELLFQEVTPG
                     AANDFEKATQIATSMVNRWGMGRDPKADEANAAGSSGRGTLSFFAAKRDASLPSELQG
                     AATRAIAAILDDAYAAARQTLIDHLDQLRRIGVYLVHQERIDGDTFDALFDGKIEVPD
                     ADVEWRPAAARPREWSSIGTVAIEAETKPD"
     gene            complement(1893..2372)
                     /locus_tag="VIF08_04030"
     CDS             complement(1893..2372)
                     /locus_tag="VIF08_04030"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY8135185.1"
                     /translation="MSRLPSLGPRGEGWVALQLLLLLAIVAAAMALPLPVGDSVEQVA
                     RVVGLGALVGGLVVFALGIVTLGSSLSPLPAPLPSAELVERGIYRFIRHPIYTGLVLA
                     ALGGSVYTVSPLAVGLTALLAVVLDLKARREEAWLRERFAGYAAYAARTKRFIPGLY"
     gene            complement(2369..3151)
                     /locus_tag="VIF08_04035"
     CDS             complement(2369..3151)
                     /locus_tag="VIF08_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEY8135186.1"
                     /translation="MGLLEGKKALIFGVANDHSIAWGIAKALHEQGAWIGFSSIEMLL
                     EKRVRPLAESIGSTFVEPCDVQKDDDIERVFSRWQREQGSLDILVHALAFARREDLDG
                     RFVDTSRDGFTLALDVSAYSMVALARAAAPLMSNGGSMMTLSYYGAEKVVANYNVMGV
                     AKAALEASVRYLAADLGPQSIRVNAISAGPIRTLAASGIAGFKQMYGRFKDIAPMRQN
                     ISIDDVGGTAVYLASDLSRNVTGETVYVDGGFNVLGVPTPEE"
     gene            complement(3163..4596)
                     /locus_tag="VIF08_04040"
     CDS             complement(3163..4596)
                     /locus_tag="VIF08_04040"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEY8135187.1"
                     /translation="MHIAMVASECEPYAKTGGLADVVDALSRALGELGHEVDVYLPRY
                     RGIELLPNTDSLELEVPMGTGIVHVALHSAQARGYRLRLVDHGYSFDRPDYYVVEPGV
                     GPDYPDNGWRFTLLGRAALETMRVESRVPDVLHGHDWEASPAILGLVHGYSSLAAFSN
                     VTTVLTCHNLAYHGWVPRSEAALQLDLPQSVGGPDAVDLLREGLIAADLATTVSPTFA
                     REALEGSVSEGVTDVLRAGAAQGRYLGIINGLDNELWDPATDTDLPARFSATDLAGKQ
                     TCRSELCTELGLDPDGPVLAMVSRLDPQKGFDLLADAMPELISMGARVCILGTGDKRL
                     VDHLKWLSGENPARLAVVDRFDRALARRMYAGADSFLMPSRFEPCGQSQMIAMRYGTP
                     PIVRFTGGLVDTVIDADYRPETGTGFGFGPAEPAALVQAVGRAMAAYADRDRWAAIQQ
                     RAMATDFSWRGPAQEYVAAYRRMLGLR"
     gene            4905..7085
                     /gene="glgP"
                     /locus_tag="VIF08_04045"
     CDS             4905..7085
                     /gene="glgP"
                     /locus_tag="VIF08_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY8135188.1"
                     /translation="MPQRIDIPTLPPQGVRLPPQLEGLNRLAYNHYWMWHPRVRVLFR
                     RIDVGSWLRYRNPVPLVQVQRDWSEILDDVDLMAEYRTLLDGFDRYMENGAGHWFDRE
                     HGAKLDGPVAYFCAEYGLHESLGIYSGGLGVLAGDHLKAASDMAIPFVGVGLFYRHGY
                     FRQTIDADGHQEHAYPDFDPRLLPVKRVAGEDGGPLRVGVELPGRTVWCAVWLADVGR
                     VPLLLLDTDIPDNDEADRPITHILYVRGREMRLHQEIVLGVGGTRALRALGIEPRAWH
                     LNEGHSALMLVERTRELMVAGMSMDDALERVRSNAVFTIHTPVSAGNERFDAALLRRL
                     AAPLVEGSGLDLERVLERGRGTDNDASQFDMTAFSLRTTNGANAVSHLHAQTANTTWS
                     SVVKEPILGITNGVHPPSWIGGPARALYESLGADLDNLDQQTKPFWNEVAKIPDAQLW
                     DAHRRQKLELAYFCRRRLQSQFARHGEAPQVLEELNEALNPDVLTIGFARRFATYKRA
                     SLIFSDEERLARILLNTERPVQIVYAGKAHPADRPGQRVIQDIFTRSRAPKFDHRVFV
                     LEDYDIRVGRYLVSGVDVWLNNPRRPLEASGTSGMKAAMNGIVNCSVLDGWWDEGYDG
                     SNGWAIGSRQTTADEGAQDWADAQDLYRLLETEIVPRFYERDANGVPTRWLEVMKAAM
                     ATTMWQFSTTRMLQEYVEQLYLPAADNGAAVEQPAAAGRASSST"
     gene            7099..>8793
                     /locus_tag="VIF08_04050"
     CDS             7099..>8793
                     /locus_tag="VIF08_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEY8135189.1"
                     /translation="MTARISLALILHNHQPVGNFGWVIGEVYERAYEPMVGALERHPG
                     VRVALHYTAPLLEWLDANKPDFIDRLRALVDRGQVEIVGGGHFEPILAALPDRDRLGQ
                     LTRMRSDLERRFGVVPRGAWLAERVWEPSLPTALVDAGYAYTVLDDNHLRGASVPEDE
                     MWGTYTTDDQGRLLTIFGTEKGLRYRIPWQPVPELIAYLRAAATEAGDRLGTMGDDGE
                     KFGGWPGTDELSWGKEQWVERCFSALEENSDWLTTVTPSDWMRSHPPIGRIYVPTASY
                     VEMTEWVLPADEAPIFGKLLHDAVEEGAPAARFLRGGMWRNFAARYREVNDLHKLMLR
                     VSAAVEAMPEGALRDRALDHLYRGQSNDCYWHGLFGGIYLVHMRMATLAELIAAEDLV
                     LGSAAASGVADYDLDGRDEVLVGTVGQTVLVDPAEGGGISAWDLRASRVALASVMRRR
                     PEAYHAKLREAEAAATAKKTKPLSPHEGYTTKEEGLAKFLVYDDHERRSGLVRVLDST
                     GVEVGDFIDGEWGVESTGQSEVVLTRSAGGLSVRKSIAVFHGRMNGSLDLDIVVTAD"
BASE COUNT         1459 a         3027 c         2981 g         1326 t
ORIGIN      
        1 acggcgcata cgtcgcgatc acgctcaacg gctcggtcgg cgacgccgcg gccgcgctcc
       61 agacgctcgg ttacgacgac ctcctgacac cggcggccca cgccgccatc aaggccagcg
      121 ccacagcccc cgccggcgac ccgctgcgcg acgccatcgg cattggcctg ctcgttctgg
      181 tcgccggcgt cgcggtctca ttcatctaca gcgcccgcga tcggctgccg gtgctgggtc
      241 gccggggcag cagattctcg gccatcatgc ctgccactcc gcgctcggcg gcgagtggtt
      301 tacccgtccc gggcaaggtg gccgccgcac cgagtgggtc gagccgcccg gtgcacctgt
      361 ccgatgtggc gggctgcgac gaagccaagc tcgagctggc cgagcccatc gagttcctgc
      421 gccaccccga cggcttccgg tcgctcggcg caaacgtgcc gcgcggcatc ctgttgtacg
      481 gcccgcccgg cacgggcaag acgatgctcg cgcgcgcggt cgcgaccgag gcgggggtgc
      541 ccttcctcca cgcgtccggt tcggacttcg tcgagaagta cgtgggcacg ggtgcgcgcc
      601 gtgtgcgcga cctgttcggg caggccaagc gcctgggcaa ggcggtcatc ttcatcgacg
      661 agttcgacgc gctgggcaag gcgcgcggcg gggccaactc gcacgaggag cgcgagcaga
      721 cgctcaacca gctgctggtc gagcttgacg gcttcggcac gtccggcgag atcgtggtca
      781 ttgcggcgac caacaggctc gacatcctcg acgaggcgct gctccggccg gggcgcttct
      841 cgcgcaaggt ccacgtcggc ctgcctgacg tcaccggccg cgaagcgatc cttgcagttc
      901 acgcgcgtgg caagccgctc gcgcccgaag tggacctgga agccctggcg cgcaagacat
      961 acggcttcag cggcgcgcag ctggccgatc tactgaatga ggcggcgatc atggccgcgc
     1021 ggcgtggatc cgcgacagtc acgaacgacg acatgcacgc gggctggctg aaggtcgccg
     1081 tgggcaccgg ccggcgccgc tccatgaacg agcgcgaccg ggcgatcatc gcggcccacg
     1141 aggtcggtca cgccatctgc ggcaaggtgc acggcgactc gcgccgcgtc gaggagatca
     1201 gcctctttgc gcatggcgac gcgctgggcg tgacggtctc gtcccaggag gacaacgacc
     1261 taccggcaga gtctgacctg cgcgcccggc tcgtggccct catgggcggg cgggcggctg
     1321 aagagctgct tttccaggaa gtgacccccg gcgccgccaa cgacttcgag aaggcgacac
     1381 agatcgcgac ctcgatggtc aaccgctggg gcatgggccg cgatcccaag gccgatgagg
     1441 cgaacgccgc cggctcgtcc ggccgcggca cgctttcctt cttcgcggcc aagcgcgacg
     1501 cctcgctgcc gagcgagctg cagggcgcgg ccacccgcgc gatcgcggcc atcctggatg
     1561 acgcgtacgc ggccgcgcgc cagacgctca tcgaccatct cgaccagctg cgccggatcg
     1621 gcgtgtacct ggtccatcag gagcggatcg acggcgacac cttcgacgcg ctcttcgacg
     1681 gcaagatcga agtgcccgac gcggacgtgg agtggcgacc agccgcggcg cgcccgcgcg
     1741 aatggtcatc gatcggcacc gtggccatcg aggccgaaac caagcccgac tgaccgccgc
     1801 ctgctcttgc gaaacgcctg atccgcgggc tgcgagtcaa ctccttggca accggtgact
     1861 cagtcactgg gctgactcgt caaagtgggc attcagtaca ggcccgggat gaacctcttg
     1921 gtacgcgcgg cgtacgcggc gtagccggcg aagcgctcac gcagccacgc ttcctcgcgc
     1981 cgcgccttga gatccagcac aacagccagc agcgcggtca ggcccacggc cagcggcgac
     2041 accgtgtaga ccgacccacc cagtgccgcc aggacaaggc ccgtatagat gggatggcgg
     2101 atgaatcggt agataccgcg ctcgaccagc tcggccgaag gcagtggggc aggcagcgga
     2161 ctgagcgagc tgcccagcgt cacgatgccc agggcgaaga cgacgaggcc accgaccaga
     2221 gcacccagcc cgaccacccg tgcgacctgc tccacggaat caccaacggg cagcggcagt
     2281 gccatggccg cggcgacgat cgcgagcagg agcagcagct ggagcgcgac ccagccttcg
     2341 ccgcgcggcc ccaggctggg taagcgactc attcttcggg tgtcggcacc ccgaggacgt
     2401 tgaagccacc gtcgacgtag acggtctcgc cggtcacgtt gcgcgacagg tcgctcgcca
     2461 ggtacacggc cgtgccgccg acgtcgtcga tggagatgtt ctggcgcatg ggcgcgatgt
     2521 ccttgaacct gccatacatc tgcttgaagc cggcgatgcc gctcgcggcg agggtccgaa
     2581 tcgggccagc agaaatcgcg ttgacgcgga tgctctgcgg accgaggtcg gccgcgaggt
     2641 agcgcaccga cgcttcgagc gccgccttcg cgacacccat cacgttgtag ttcgcgacga
     2701 ccttctcggc gccgtagtag ctgagcgtca tcatcgagcc gccgttcgac atcaggggtg
     2761 cggccgcgcg agccagcgcg accatggagt aggcgctcac gtcgagggcg agcgtgaagc
     2821 cgtcgcgcga cgtgtcgacg aaacggccgt ccaggtcctc gcgccgcgca aatgccagcg
     2881 catggacgag tatgtcgagc gacccctgct ctcgttgcca gcgcgaaaag acgcgctcga
     2941 tgtcgtcgtc cttctggacg tcgcacggct cgacaaacgt tgacccaatc gactcggcga
     3001 gaggccggac tcgcttctcc aggagcatct cgatcgacga gaagccgatc cacgcaccct
     3061 gctcatgcaa cgccttggcg atgccccagg cgatcgaatg gtcattggcg acgccgaata
     3121 tgagcgcctt cttaccttca agcagtccca tggcggagaa cgttagcgca ggccgagcat
     3181 ccggcgatag gcggccacgt actcctgggc ggggccgcgc cacgagaagt cggtcgccat
     3241 cgcgcgctgc tggatagccg cccagcgatc gcgatcagcg tatgcggcca tggcccggcc
     3301 gactgcctgg accaatgccg cgggctcggc cgggccaaag ccaaagcccg tgcccgtctc
     3361 aggccggtag tcggcatcga tcacggtgtc gaccagcccg cccgtgaaac gcacgatggg
     3421 cggcgtgccg tagcgcatcg cgatcatctg gctctggcca cacggctcga aacgcgatgg
     3481 catcaggaat gagtctgcgc cggcatacat gcgccgcgcc agagcacgat cgaaccgatc
     3541 gaccaccgcc aatcgtgccg ggttttcgcc cgacagccac ttgaggtgat cgaccaggcg
     3601 cttgtcgccg gtgcccagga tgcacacccg agcgcccatg ctgatcagct ccggcatcgc
     3661 gtccgcgaga aggtcgaagc ccttctgtgg gtcgagccgg ctgaccatcg ccaggacggg
     3721 gccgtccgga tcgagtccca gctccgtgca cagctccgat cgacacgtct gcttgccggc
     3781 caggtccgtt gccgagaacc gggcgggtag atcggtgtcc gtcgccggat cccagagctc
     3841 gttgtcgagg ccgttaatga tgcccaggta gcggccctgt gcggcgccgg cgcggaggac
     3901 atcggtcacg ccctcgctga ccgatccctc gagcgcctcg cgcgcgaacg tggggctgac
     3961 cgtcgtcgcc aggtccgcgg ctatgagccc ttcacgcagc aggtccacgg catccggacc
     4021 accgacggac tgcggcaggt ccagctgcag agcggcctcc gagcgcggta cccagccgtg
     4081 gtaggcgagg ttgtggcacg tcagcaccgt cgtgacgttg ctgaaggccg cgagcgagct
     4141 atagccgtgg acgaggccca ggatcgcggg cgaggcttcc cagtcgtggc cgtggagcac
     4201 gtctggcacg cggctctcga cgcgcatcgt ctccagcgcc gcgcggccga gcagtgtgaa
     4261 gcgccagccg ttgtcggggt agtcggggcc tacgcccggt tcgacgacgt agtagtcggg
     4321 gcgatcaaac gagtacccat gatcaacgag gcgcaggcgg tagccccgcg cctgcgcgct
     4381 gtgcagcgcg acatgcacga tgcccgtacc catgggcacc tcgagctcga ggctgtcggt
     4441 gttcggcagc agctcgatgc cgcggtagcg gggcaggtaa acgtcgacct cgtggcccag
     4501 ttcgcccaac gcccgcgaga gcgcgtcaac cacgtcggcc aggccgcccg tcttggcgta
     4561 cggctcacat tcagaggcga ccatggcgat gtgcatgacc ggccgatgct aactgggcca
     4621 acggggcggt ccttaaggcc aaccatgtcg cgtgacacca ctatcgtcac gaagcgggct
     4681 aacgcgtcgg gcgcgcgagg tttgtggcgc tgatgagccc gagctgagcg tgagcaaccg
     4741 gttgcggaac tgtctcgggc ggctgatcgg ccggcaggcg ccccactaag cggctcgttg
     4801 accatagctg cgtcatggag tcgcgcgaag caacggccgg ggcggcgcga agcatgctga
     4861 accggtttac gacgccaatc gacgtgcgct agcatctccc tcccgtgcct caaaggatcg
     4921 acattccaac cctgccgccg cagggcgtcc gactaccccc tcagctcgaa gggttgaacc
     4981 ggctcgcgta caaccactac tggatgtggc acccgcgggt gcgcgtcctt ttccggcgca
     5041 tcgacgtcgg ctcgtggctg cgctatcgca acccggtgcc gctggtccag gtgcagcgcg
     5101 actggtcgga gatcctcgac gacgtcgatc tgatggccga gtaccggacg ctgctcgatg
     5161 gcttcgaccg gtacatggag aacggggcgg ggcactggtt cgaccgcgag cacggcgcca
     5221 agctcgatgg gccggtcgcc tacttctgcg ccgagtacgg cctgcacgag tcgctgggca
     5281 tctactcggg cggcttgggc gtgctcgccg gcgaccacct gaaggcggcc agcgacatgg
     5341 ccatcccgtt cgtcggtgtc ggtctcttct atcgccatgg ctacttccgc cagaccatcg
     5401 atgccgacgg ccaccaggaa cacgcctatc ccgacttcga cccgcgcctc ctgccggtca
     5461 agcgcgtggc cggcgaggac ggcggtccgc tgcgcgtcgg cgtcgagctg cccgggcgca
     5521 cggtgtggtg cgcggtttgg ctggccgatg tgggccgagt gcctctgctc ctgctcgaca
     5581 cggacatccc cgacaacgac gaggccgacc ggccgatcac tcacatcctg tacgtgcgcg
     5641 ggcgcgagat gcgcctccac caggagatcg tcctgggcgt cggcgggacc cgcgcactgc
     5701 gcgcactggg gatcgagccg cgggcgtggc acctcaacga aggccactca gcgctgatgc
     5761 tcgtcgagcg cacgcgcgag ctgatggtcg ccggcatgtc catggacgat gccctggagc
     5821 gtgtcaggag caacgccgtc ttcaccatcc acacgccggt ctccgcgggc aacgagcggt
     5881 tcgacgctgc gcttctacgc cggctggccg ctcccctcgt tgaaggctcg ggcctggacc
     5941 tcgagcgcgt cctggaacgt ggccgcggca cggacaacga cgccagccag ttcgacatga
     6001 cggccttctc gctgcgcacc accaacggcg cgaacgcggt gtcgcacctc catgcgcaga
     6061 cggccaacac gacctggtcg agcgtcgtca aggagcccat cctgggcatc accaacggcg
     6121 ttcaccctcc gtcctggatc ggcggtcccg cgcgcgccct gtatgaatca ctgggcgccg
     6181 acctcgataa cctcgaccag cagaccaagc ccttctggaa cgaagtcgcg aagatccccg
     6241 acgcccagct gtgggatgcg catcgccgcc agaagctcga gctggcgtac ttctgccggc
     6301 gccgcctgca gtcgcagttc gcgcgccacg gcgaggcgcc ccaggtgctc gaggagttga
     6361 acgaggcgct gaacccggac gtgctgacga tcgggttcgc gcgacggttc gcgacctaca
     6421 agcgcgcgtc cctgatcttt tccgacgagg agcgcctggc gcgcatcctg ctcaacactg
     6481 agcggcccgt ccagatcgtc tacgccggca aggcacaccc cgccgaccgg cccggccagc
     6541 gcgtcatcca ggacatcttc acgcgcagcc gcgcaccgaa gttcgaccac cgcgtcttcg
     6601 tcctggagga ttacgacatc cgcgtcgggc gctacctcgt gagcggcgtt gacgtctggt
     6661 tgaacaaccc gcgccgtccg ctcgaggcga gtggcacgag tggcatgaag gcggccatga
     6721 acggcatcgt caactgctcg gtcctggacg gttggtggga cgagggctac gacggttcga
     6781 acggctgggc aatcggatcg cgccagacga ccgccgacga aggcgcccag gactgggccg
     6841 atgcacagga cctctaccgc ctgctcgaga cggagatcgt gccgcgcttc tacgagcgcg
     6901 acgccaacgg cgtgcccacg cgctggctgg aagtcatgaa ggccgcgatg gccacgacca
     6961 tgtggcagtt ctcgaccacg cgcatgctcc aggagtacgt cgagcagctg tacctgccag
     7021 cggccgacaa cggcgccgcg gtcgagcaac ccgccgcagc cggacgagcc tcgtcttcca
     7081 cgtaacggcc gcgccccaat gacggcgcgc atctcgctcg cgctcatcct ccacaaccac
     7141 cagccggtgg gcaatttcgg ctgggtcatc ggcgaggtct acgagcgcgc ctacgagccc
     7201 atggtcggtg ccctggagcg tcacccgggc gtccgcgtgg cgctgcacta cacggcgcca
     7261 ttgctcgagt ggctcgacgc gaacaagcct gacttcatcg accgattacg ggcactggtt
     7321 gaccgcggcc aggtggagat cgtgggcggc ggccatttcg agccgatcct cgccgcgctg
     7381 cccgaccgcg accggctggg ccagctgacg cggatgcgct cagatctgga acggcgattc
     7441 ggcgttgtgc cgcgcggcgc ctggctcgcg gagcgcgtct gggagccgtc actgccaacc
     7501 gcgctcgtcg atgccggcta cgcctacacc gttctcgacg acaaccacct gcgtggtgcg
     7561 tcggtgccgg aggacgagat gtggggcacc tacacgaccg acgaccaggg ccggctgctg
     7621 accatcttcg gcacggagaa gggcctccgc taccggatcc cgtggcagcc ggtgcccgaa
     7681 ctgatcgcgt acctgcgcgc ggcagcgacc gaggccggcg atcgcctggg gaccatgggc
     7741 gacgacggcg agaagttcgg cggctggccg ggcaccgacg aattgagctg gggcaaggag
     7801 caatgggtgg agcggtgctt cagcgccctg gaggaaaact ccgactggct gaccacggtc
     7861 accccgtccg actggatgcg ctcccaccca ccgatcggtc ggatctacgt gcccacggcc
     7921 tcgtacgtcg agatgaccga atgggtgctg cctgctgatg aggcaccgat tttcggcaaa
     7981 ctcctccacg acgcggtcga ggagggcgca ccggcggcgc gcttcctgcg cggcggcatg
     8041 tggcgcaact tcgcggcgcg ttatcgcgag gtcaacgacc tgcacaagct gatgctgcgc
     8101 gtgtccgccg cggtcgaggc gatgcctgaa ggtgcgctac gcgaccgggc cctggaccac
     8161 ctgtatcgcg gccagtcgaa cgactgctac tggcacggcc tcttcggtgg catctacctg
     8221 gtgcacatgc gcatggcgac actcgccgag ttgatcgccg cggaagacct ggtcctgggc
     8281 tcggcggccg cgagtggcgt ggccgactac gacctcgacg gccgcgacga ggtgctcgtc
     8341 gggactgttg ggcagacggt gctcgtcgat cctgctgaag gcggcgggat cagcgcgtgg
     8401 gacctccgcg cgtcgcgcgt cgcgctggcg tcggtgatgc gccgccggcc ggaggcatac
     8461 cacgccaagc tgcgcgaggc ggaggcggcg gccacggcca agaagacgaa gccgctcagc
     8521 ccacatgagg gttacacgac gaaggaagag ggtctcgcca agttcctcgt ctacgacgac
     8581 cacgagcgac ggagcgggct ggtgcgagtc ctcgattcga caggcgtcga agtcggcgac
     8641 ttcatcgacg gcgagtgggg ggtcgaatcg acaggtcagt cggaggtcgt gctcaccaga
     8701 tctgctggcg ggctatcggt ccgcaagtcg atcgcagttt ttcacggccg gatgaatggc
     8761 tcgcttgacc tggacatcgt cgtcaccgct gac
//
LOCUS       DATBRX010000113         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2716163, whole genome shotgun sequence.
ACCESSION   DATBRX010000113 DATBRX010000000
VERSION     DATBRX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2716163"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..2559
                     /locus_tag="VIF08_04055"
     CDS             118..2559
                     /locus_tag="VIF08_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8135190.1"
                     /translation="MSKRLPSGTVTLLFTDIEGSTRLLRKLEAGYAEALAGHRRLLRD
                     AFAKHGGVEVDTQGDALLVAFANAADAVIAAEDGQAALAGGQIAVRMGIHTGLPDLTG
                     EGYVGLDLHLGARIGAAGHGGQVLMSKATADLVGARAELADLGEHRLKDFPEPVWLYQ
                     LGRTAFPPLRTISNTNLPRSVSTFIGREREIAGIKAVLFGGARLVTLTGPGGTGKTRL
                     AIETAASLMPEFRNGVFWIDLAALRDPTLVMGEIVRTIGARDDLSEHIGERQMLLVLD
                     NLEQLVDAAPEVAALLGKCPNLAVLATSREVLRIGGEAEFPVPVLAGSEAVTLFAERS
                     GLSPSPEAAELCRRLDNLPLAVELAAARASVLSVAQITARLADRLDLFKGGRDADPRQ
                     RTLRATIEWSHDLLSVSEQRLFRALSVFAGGWALEAAEDVCGADIDTLQSLADKSLIN
                     HVNERFGMLESIREYAAARLAESDDLGIAHRHGRWFAELAERAEPLLEGGDQKVWLDR
                     LELENDNIRCALSGAIAGGDGELALRLAAAVATFWWIHGHWNEGRRWFELALGQRPER
                     DTLRVRALEGAAHLAQRQLDLGLAEQMAREALSISTELGDSSRTARVLRILGLTATAA
                     GDRDRFRTLTEQSADAGRASGDDWALLMAQMNLGYARLEDGDSTGAIEFFDDALALAA
                     GRGDERSQSHVLENRAWAWVDQGHLDRARADFAASLRLAHVLNFTEIAAEDLSGIAAI
                     LAARGELAEAALTLSGAQHLREQIGSDADTVENAMEERTVAAIERGLAQPEREAASAA
                     GRKLDLDGLVARALG"
     gene            2569..3093
                     /locus_tag="VIF08_04060"
     CDS             2569..3093
                     /locus_tag="VIF08_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8135191.1"
                     /translation="MSASTVLIIPDPALVLLIGPAGAGKSTFAAAHFSPVEIVSSDHL
                     RGLLANDPADQTASADAFRILTLMVAGRLKRRLTTVVDATSLRAANRRKYRSLAARHG
                     IPVVAIAFDLSPRAYHDRNRGRTDRVVDEDVVERQIEGMARTMAALPLEGYAALYVLR
                     DPHESIIAKRHGSA"
BASE COUNT          540 a         1072 c         1144 g          559 t
ORIGIN      
        1 ctggccgcag ccgcagcgct cgatcccgag ctggccgacc ggctcgacga gatccgcccg
       61 ctcggggagt ggctgcggct gtcggctgga gctgagtcgg cggcctgact gacccgcatg
      121 tctaagcgcc tgccaagcgg caccgtgacc ctcttgttca ccgacataga ggggtcgacc
      181 aggctcctgc gtaagttgga ggccgggtat gccgaagctt tggccggaca tcgccgacta
      241 ttgcgcgacg cctttgcgaa gcacggtggt gtcgaggtcg acacgcaggg cgatgcgctg
      301 ctggtcgcat tcgccaatgc cgcggacgcc gtgatcgcgg ccgaagacgg ccaggcggca
      361 ctggcgggcg gccagatcgc cgttcgaatg ggcatccaca ccggccttcc ggacttgaca
      421 ggcgagggct atgtcggtct cgacctccat ctgggcgcgc ggattggagc cgcaggtcat
      481 ggtggccagg tgctgatgtc aaaggccaca gctgacttgg tcggcgcgcg cgccgaatta
      541 gccgacctgg gcgagcaccg actcaaggac ttccccgagc cggtctggct ctaccagctc
      601 ggacggacag cattcccacc gctgcgcacg atctcgaata cgaacctgcc tcgatcggtg
      661 tcgacattca tcggccgaga gcgcgagatc gcgggcatca aagccgtgct gttcggcggc
      721 gccaggctcg tcaccctgac tgggcctggc ggcactggca agaccagact ggcaatcgag
      781 acggctgcct cgctcatgcc cgaattccgc aacggcgtgt tctggataga cctcgcggcg
      841 ctgcgcgatc cgacgctcgt catgggcgag atagtgcgaa cgattggtgc ccgcgatgac
      901 ctttccgagc acattggaga gcgccagatg ctgctcgtgc tggacaacct cgagcaactc
      961 gtggacgccg cgcccgaagt cgcagcgctc ctcggcaagt gtcccaacct ggcggtgctg
     1021 gcgaccagcc gagaggtgct gcggattggc ggcgaagccg aattcccggt accggtactt
     1081 gcaggatcag aggcggtgac attgtttgcc gagcgttctg gcctgtcacc tagtccggag
     1141 gcggcggagc tgtgtcgacg cctcgacaac ctgcccctcg ccgtcgaact cgccgccgcc
     1201 cgggcgagcg tgctgagcgt cgcgcagatc acagctcgcc tcgcggaccg tcttgacttg
     1261 ttcaagggcg gtcgcgatgc cgacccccga caacgcacct tgcgggcgac catcgagtgg
     1321 tcgcacgacc tgctttctgt atccgagcaa cggctatttc gggcgctctc tgttttcgcg
     1381 ggcggctggg cgctcgaggc tgctgaggat gtctgcgggg ccgacatcga cacgctccag
     1441 tctctggccg acaagagcct gatcaatcac gtcaacgagc gctttgggat gctcgaatcg
     1501 atacgcgagt atgcggccgc tcggttggcc gagtccgatg accttggcat tgcgcaccgg
     1561 cacggccgct ggttcgccga gctagctgag cgtgccgagc cgctccttga gggcggagac
     1621 caaaaggtgt ggctcgaccg gttggagctg gagaacgaca acatcaggtg cgcgttgtcg
     1681 ggagcgatcg ccggcggcga cggcgagctg gctttgaggc tggccgctgc tgttgcgacg
     1741 ttctggtgga ttcacgggca ttggaacgaa ggccggcgct ggttcgagct ggcccttggg
     1801 caacgcccgg aacgcgacac cctgcgcgtc agggcgctcg agggagcggc gcacctggcc
     1861 cagcgtcaac ttgacctcgg gcttgccgag cagatggcga gggaggccct gtcgatttcg
     1921 accgagctgg gagattcgtc gagaacggcg cgagtcctgc gcatcctggg cttgaccgca
     1981 acggccgccg gcgatcgaga tcggtttcgc acgcttacgg agcaaagtgc cgacgcgggt
     2041 cgcgcgagcg gcgacgattg ggcactgctc atggcccaaa tgaacctggg ctacgctcgg
     2101 ctggaagacg gcgacagcac aggcgcgatt gaattcttcg acgacgcgct tgcgctggcc
     2161 gccggccgcg gcgacgaacg cagccaatcg cacgtccttg agaatcgcgc gtgggcgtgg
     2221 gtcgaccagg gacatcttga ccgggcacgc gctgatttcg cggccagcct gcgactcgcc
     2281 cacgtgctga atttcacgga gatcgccgcc gaagacctga gcggaatcgc cgccatcttg
     2341 gccgcacgcg gcgaactcgc ggaggcggcc ttgacgttgt cgggggcaca gcacctgcgc
     2401 gagcagatcg ggtcagacgc ggatacggtc gagaacgcga tggaggagcg gacagtcgcg
     2461 gccatcgaac gaggcctggc ccagccagaa cgtgaggccg catccgccgc cggccgcaag
     2521 cttgacctcg atggcctggt agcacgtgcg ctgggctgac acgcgcacgt gagcgcgagc
     2581 accgtgctca tcatccccga cccggcgctg gtccttttga tcgggccggc gggggcgggc
     2641 aagtcgacct tcgcggcggc gcacttcagc ccggtcgaga tcgtctcgtc cgatcacctg
     2701 cgcgggctgc tggcgaacga cccggccgac cagacggcct cagctgatgc cttccggatc
     2761 ctgacgctga tggtcgccgg ccgactgaag cggcgcctga cgacggtggt cgacgcgacc
     2821 tcgctgcgcg ccgcgaaccg gcgcaagtac cgatcgctgg cagcgcgcca cggcatcccg
     2881 gtcgtggcga tcgcgttcga cctctcgccg cgcgcctacc acgatcggaa tcgcggccgg
     2941 accgaccggg tcgtcgacga ggacgtcgtc gagcggcaga tcgaggggat ggcccggacc
     3001 atggccgcgc tgccgctgga gggctacgcg gcgctttacg tgttgcgcga cccgcacgag
     3061 tcgatcatcg cgaaacgcca tgggtcggcc tagctcgcgg ttgtttcccg ggatgcaagt
     3121 atcggccaac aagacacgaa gctgagcctc ttcttgcccg cgtgtttcgt ggctgataac
     3181 gctcggccag ctttgagccg gtttcaggcc tgtctgttgc ctaacttggc ttttgtttcg
     3241 cccgggtgaa acaaagagcg cgcgaggtgg ctagtcctcg cgccgggttt cgtccacgcg
     3301 gcgcagcgcc cacgc
//
LOCUS       DATBRX010000114         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2380933, whole genome shotgun sequence.
ACCESSION   DATBRX010000114 DATBRX010000000
VERSION     DATBRX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380933"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..943)
                     /locus_tag="VIF08_04065"
     CDS             complement(311..943)
                     /locus_tag="VIF08_04065"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY8135192.1"
                     /translation="MKRSISVPGVGRVSIQPDIATLRLGVLVVRQTAAAARESAAATM
                     TAVLEALSANAVAKKDLRTALLSLSPVTDYSPETGPRVTGYQAANSVSVTVRDLTSAG
                     ALIDAALAAGATSMDGLDFTVDDPSAAEEQARQLAMADAKRRAQTIAAASGVTLGPVI
                     GVIEGDRGGAPVPFPGPRAMAFKAEAADTPVEAGSQEIVVSVSVSFAIDA"
     gene            967..1830
                     /locus_tag="VIF08_04070"
     CDS             967..1830
                     /locus_tag="VIF08_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8135193.1"
                     /translation="MAKLQKRNLGSPDELRTVGRGRLEVVEMGDAAFGRVTFPPGFRW
                     SEDVKPIVGTDYCEVHHVGYTISGRLHSEMRDGSTLDVGPGDIFEIPPGHDAWVVGDE
                     PWVSVDWTGRRFYGKTPDADAERVLATILFTDIVGSTERLREMGDQAWRALLADYHAM
                     LRRALERHRGLEVQTTGDGVLARFDSPARAVRCALETADGVRQLGLEQRAGLHTGEIE
                     LAGDDVRGVAVHLAARVAAAAGAGEVLVSGTTNGLLFGSGLTTVSRGNHSLKGIDQPV
                     ELFAVTGSATA"
     gene            1844..>2643
                     /locus_tag="VIF08_04075"
     CDS             1844..>2643
                     /locus_tag="VIF08_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8135194.1"
                     /translation="MKQHQVAMLGAGFISDFYAMTLHAQRSRDQVRIVSSRDAARGKA
                     FCERWGVPESTTDMAAAVNHPDVDVVVIGLPNYVHQEAVELAAKAGKAVLCTKPLGRT
                     AAEAKTMLDAVEKAGVFAGYLEDLCYTPKTLKAVKAVRAGAIGEVTWVRSRETHPGPH
                     SAWFWDGRLTGGGAIVDLGCHCVEIIRNYVGKGVRPVEVMCHADTLVHPIADEDNAVA
                     LIRFENGAIGQFEVSWTFRGGMDLRDEVAGTAGTIWTNHFLRTGFEMFT"
BASE COUNT          415 a          854 c          962 g          412 t
ORIGIN      
        1 agctcgaagc cggcgatcat gcgcagcgtc gtggtcttgc cgctgccgga cgggccgagc
       61 atcgagaaga actcgccgtc gcgcacgtcg agctcgattc cgtctacggc aacgacgtcg
      121 ccgaacttct tgaccacatg gtcgaggcga accgcgctgt ccgacgtaac ggccaattgc
      181 tctccgcagg tatggctctg cctgagcgga cgaatgagtg cgcgcgctcg atcccctggt
      241 ggcgcgctcc cgctggcgcg ggcgtgggct aattatcggt gcgggtctcg agcgatgtcg
      301 agcctgaatg tcaggcgtcg atggcgaacg aaacgctgac gctgaccact atctcctggc
      361 tgccagcctc gaccggggtg tcggccgcct ctgccttgaa cgccatggcg cgcggcccgg
      421 gaaaaggcac gggcgcgccg ccgcgatcgc cttcgatcac accgatcacg gggccgaggg
      481 tcacgcccga cgcggcggca atggtctggg cgcggcgctt cgcgtcggcc atggcgagct
      541 ggcgcgcctg ctcttcggcc gcgctcgggt cgtcgacggt gaagtcgagc ccatccatgc
      601 tggtcgcgcc ggcggcgagc gcggcgtcga tcagggcgcc cgccgatgtg agatcgcgca
      661 cggtgacgct gaccgagttg gcggcctggt agccggtgac gcgcggcccg gtctccggtg
      721 agtaatcggt gaccggactg agcgaaagga gggccgtgcg caggtccttc ttggcgacgg
      781 cattggcgct gagcgcctcg aggaccgcgg tcatcgtcgc ggcggccgat tcgcgtgcgg
      841 cggccgcggt ctgacgcacg accagcacgc ccaagcgcag cgtggcgata tctggctgga
      901 tggaaacgcg gccgacgccg gggactgaaa tcgatcgctt catacgccca atcctagaat
      961 cggaacgtgg caaagctcca aaagcgaaat ctgggctcgc ccgatgagct gcgaacggtg
     1021 ggccggggcc ggctcgaggt ggtcgagatg ggcgacgcgg ccttcggtcg ggtgaccttc
     1081 ccgccgggct ttcgctggtc ggaggacgtc aagccgatcg tcggcaccga ctactgcgaa
     1141 gtccatcacg tcggctacac gatcagcggc cggctccatt cggagatgcg cgacggctcg
     1201 acgctcgacg tggggccggg cgatatcttc gagatcccgc cgggccacga cgcctgggtc
     1261 gtgggtgacg aaccgtgggt gagcgtcgac tggacggggc ggcgcttcta cggcaagacg
     1321 cccgacgcgg acgctgagcg ggtcctggca acgatcctct tcacggacat cgtgggctcg
     1381 accgagcggc tgcgcgagat gggcgaccag gcgtggcgcg cgctgctggc cgattaccac
     1441 gccatgctgc ggcgagcact cgagcggcat cgcgggctgg aggtgcagac gacgggtgat
     1501 ggcgttctgg cgcgattcga ttcgccggcg cgcgccgtgc gctgtgccct ggaaacggcc
     1561 gacggagtgc gccagctggg cctcgagcag cgcgctggcc tgcacaccgg cgagattgag
     1621 cttgccggtg acgacgtgcg cggcgtcgcg gtccacctcg cggcccgcgt cgcggccgcg
     1681 gccggcgctg gtgaggtgct cgtgtcaggc acgaccaatg gccttctgtt cggctctggc
     1741 ctaacgacag tcagtcgtgg taaccattca ctgaagggga tcgatcagcc ggtggagctc
     1801 ttcgcggtca ccggcagcgc cacagcctga ggaggggcga cacgtgaagc agcaccaggt
     1861 ggccatgctc ggtgcggggt tcatcagcga cttctacgcc atgacgctcc acgcgcagcg
     1921 gtcgcgcgac caggtgcgca tcgtctcgtc gcgcgacgcc gcgcgcggca aggccttctg
     1981 cgagcgctgg ggcgtgcccg agtcgacaac ggacatggcc gccgcggtga atcaccccga
     2041 tgtcgacgtg gtcgtgatcg gcctgccgaa ctacgtgcac caggaagcgg tcgagctggc
     2101 ggccaaggcc ggcaaggcgg tgctgtgcac caagccgctg ggtcggacgg ccgcggaagc
     2161 gaagaccatg ctcgacgcgg tcgagaaggc gggcgtgttc gcgggctacc tcgaggatct
     2221 gtgctacacg cccaagactt tgaaagcggt caaagccgtg cgcgccgggg cgattggcga
     2281 ggtgacctgg gtgcgctcgc gcgaaaccca ccccggaccc cactcagcgt ggttctggga
     2341 cggccgattg accggcgggg gtgccatcgt cgacctgggc tgccactgcg tcgagatcat
     2401 ccgcaactac gtcggcaagg gcgtgcgccc ggtcgaggtg atgtgccacg ccgacacgct
     2461 ggtccatccc atcgccgacg aggacaacgc ggtcgcgctc atcaggttcg agaacggcgc
     2521 catcggccag ttcgaggtgt cgtggacgtt ccgcggcggc atggacctgc gcgacgaggt
     2581 cgccggcacg gcaggcacga tctggaccaa tcacttcctg cgcaccggct tcgagatgtt
     2641 cac
//
LOCUS       DATBRX010000115         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_258936, whole genome shotgun sequence.
ACCESSION   DATBRX010000115 DATBRX010000000
VERSION     DATBRX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258936"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VIF08_04080"
     CDS             complement(<1..723)
                     /locus_tag="VIF08_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8135195.1"
                     /translation="MARPDFRGTAARRLAEERDLSPAILSLISPEGTERPLGVRNIPL
                     DRITPNPDQPRMNFDEAALAELASSIREHGVLQPILVRPTGELHRYQLVAGERRWRAA
                     KAAELEVIPALIEQLDDETALEIGIIENLQREDLSPLEEAMIYDRMTHEHGYSVRRLA
                     QKIGKDKGYVENRLRLAGAPTEIKQLVSLRKDTLSHAYELLKVEDAKKRRKLAEQVAS
                     GELSLVKLREKIEGRPARKPGAK"
     gene            complement(716..1486)
                     /locus_tag="VIF08_04085"
     CDS             complement(716..1486)
                     /locus_tag="VIF08_04085"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8135196.1"
                     /translation="MARIIAIANQKGGVGKTTTAINLAGSLAEQGYRVLCVDMDPQAN
                     LSVGLGINLRNVENAIGDVLVGNGVGLDQIIQQTQTTGIDVAPATIDLSAAENQLFSA
                     IGREQTLREAFANSSAETYDYVVIDCPPTLGLLTLNALVASDGVIIPVQTQYYALKGF
                     SALMNVITQIRTKGLNPELRVLGLLPTFYDGRTVLGRDMLQELRDMGDHHIFENMIKQ
                     TVRLGEAPLVGRPVTSYASNTEAARAYRGLAREVIELG"
     gene            1615..3168
                     /locus_tag="VIF08_04090"
     CDS             1615..3168
                     /locus_tag="VIF08_04090"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY8135197.1"
                     /translation="MAPKKERFDRLRDMRAQAQLGGGRERIDQQHERGKLTARERLEL
                     LLDPGSFVEFDAFVTHRATDFGLEAQRYLGDGVVTGHGTIDGRLVFVFSQDFTVFGGS
                     LSEAHAEKICKVMDLAVRVGAPVIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGVV
                     PQISLVLGPCAGGAVYSPAITDFTIMVEGTSYMFVTGPDVVKTVTHEDVDREHLGGAS
                     VHTMISGVAHLAAADEDAAMTLARRILDHLPQNNLEKPADRPTTDPRDRRDPALDAVV
                     PDEANQPYDMHEVITRVVDDADFLEIQPAWAANIIVGFARLGGHSVGIVANQPAVLAG
                     ALDIDASDKAARFVRTCDAFNVPLLTFEDVPGFLPGVHQEHGGIIRHGAKLLYAYAEA
                     TVPKLTVITRKAYGGAYDVMSSKHIRGDVNLAWPTASIAVMGPEGAVNIIFRDEIAAA
                     TDAAGERVRLRTEYEERFANPYIAAGRGYIDDVIMPADTRPRLIAELEMLAGKRQVNL
                     PRKHGNIPL"
BASE COUNT          541 a         1088 c         1109 g          584 t
ORIGIN      
        1 ctttgctccc ggcttacgcg ccggccggcc ctcgatcttc tcgcgcagct tgaccagtga
       61 cagctcgccg ctcgcgacct gctcggccag cttgcgccgc ttcttggcgt cctcgacctt
      121 cagcagctcg taggcgtgcg acagcgtgtc tttgcgcaaa gacacgagct gcttgatctc
      181 cgtcggggcg cccgccaggc gaaggcggtt ctcgacgtag cccttgtcct tgccgatctt
      241 ctgggcgagc cggcgcacgg agtagccgtg ctcgtgggtc atgcggtcgt agatcatcgc
      301 ctcctcgagc ggcgacaggt cctcgcgctg caggttctcg atgatgccga tctcgagtgc
      361 cgtctcgtcg tcgagctgct cgatgagcgc cgggatgacc tccagctcgg ccgccttggc
      421 cgcgcgccag cgccgttcgc cggccaccag ctggtagcgg tgcagctcac cagtcggtcg
      481 gacgaggatc ggctggagca cgccgtgctc gcggatcgac gatgcgagct ccgcgagggc
      541 cgcctcgtcg aagttcatgc gcggctggtc ggggttgggc gtgatgcgat cgagcgggat
      601 gttacggacg ccaagcggac gctccgttcc ctcgggcgag atgaggctca ggatggccgg
      661 tgagaggtcg cgctcctccg cgagacggcg tgctgcggtg ccgcggaaat ctggtctagc
      721 caagttcgat cacctcccgc gccagccccc gataggcgcg cgccgcttca gtgttgctgg
      781 catatgaggt caccggtcgc ccgaccagcg gcgcctcgcc cagcctgacg gtctgcttga
      841 tcatgttctc aaatatgtgg tgatcgccca tgtcgcgcag ctcctggagc atgtcgcgcc
      901 cgagcaccgt gcgaccgtcg tagaacgtgg gcagcaggcc cagcacgcgc agctcggggt
      961 tgaggccctt ggtgcggatc tgggtgatca cgttcatcag ggcgctgaag cccttgagcg
     1021 cgtagtactg cgtctggacc ggaatgatga cgccgtcgga tgcgacgagc gcgttgagcg
     1081 tcagcaggcc gagtgtcggc gggcaatcga tgaccacgta gtcatatgtc tccgcgctcg
     1141 aattggcgaa tgcctcgcgc agtgtctgct cgcggccgat cgcgctgaag agctggttct
     1201 ccgccgcgga caggtcgatg gtggccggtg cgacgtcaat gccagtggtc tgcgtctgct
     1261 ggatgatctg gtccagtccg acgccgttgc cgacgaggac atcgccgatg gcgttctcga
     1321 cgttgcgcag gttgatgcca agaccgaccg acaggttggc ctgtgggtcc atgtcgacac
     1381 agagaacgcg ataaccctgc tcagcgagcg agccggcgag gttgatcgcc gtggtggtct
     1441 tgcccacgcc gcccttttga ttggcgatgg cgatgatccg tgccaccaaa accctcctac
     1501 ccaagtggat cgtcaatcta gaagcatcgc gcgcgcgaca ccatgcggtt atccacagga
     1561 tgatggactt gttcaccgtt tctgtggaca tctcgcgctt gctaatcttg cgccgtggcc
     1621 ccaaagaagg agcgtttcga tcgcttgcgc gacatgcgcg cgcaggcgca gctcggtggt
     1681 gggcgggaac gcatcgatca gcagcacgaa cgcggcaagc tgactgcgcg cgagcgcctg
     1741 gagctgctgc tcgatcccgg ctcgttcgtc gagttcgacg cattcgtcac tcatcgcgcg
     1801 acggatttcg gcctcgaggc gcagcgctac ctgggcgacg gggtcgtcac aggccacggc
     1861 acgattgatg gccggcttgt tttcgtgttc agccaggact tcacggtctt cggcggatcg
     1921 ctctcagagg cgcacgcgga aaagatctgc aaggtgatgg atctcgcggt gcgcgtgggt
     1981 gcgccggtca tcggcctgaa tgactccggt ggcgctcgca tccaggaagg tgtcgtgtcg
     2041 ctgggtggct atgccgacat cttcctgcgt aacacgctcg cgtctggtgt cgtgccgcag
     2101 atctcgctgg ttctgggccc atgcgccggg ggagcggtct actcgccggc gatcaccgac
     2161 ttcacgatca tggtcgaagg cacgagctac atgttcgtga ccgggccgga cgtggtgaag
     2221 accgtcaccc acgaagatgt cgaccgcgag catctcggtg gcgcgagcgt gcacacgatg
     2281 atcagcggcg tcgcccatct cgcggcggcg gacgaggacg cggcgatgac gctggcccgg
     2341 cgcatcctcg accacctgcc acagaacaac ctagagaaac cggccgaccg acccaccacc
     2401 gacccacgcg accgacggga cccagccctc gacgccgtcg tgcccgacga ggccaaccag
     2461 ccgtacgaca tgcacgaggt gatcacgcgc gtcgtcgatg acgcggactt cctcgagatc
     2521 cagccggcgt gggcggcgaa catcatcgtc ggcttcgcgc gcctgggcgg gcacagcgtg
     2581 ggtatcgtgg ccaaccagcc ggccgtgctc gcaggtgcgc tggacatcga cgcctccgac
     2641 aaggcggcgc gcttcgtgcg cacctgcgac gcgttcaacg tgccgctgct cactttcgag
     2701 gacgtgcccg gcttcctgcc cggcgttcac caggagcacg gcggcatcat ccgccacggc
     2761 gccaagctgc tctacgccta cgccgaggcg accgtgccca agctaaccgt gatcacacgc
     2821 aaggcctacg gcggcgccta cgacgtcatg agcagcaagc acatacgcgg cgacgtcaac
     2881 ctcgcctggc caacggcatc aatcgcggtg atgggccctg aaggggcggt caacatcatc
     2941 ttccgcgacg agatcgcggc ggcgacagat gcggccgggg agcgagtgag actgcggacc
     3001 gagtacgagg agcgcttcgc caatccttac atcgccgcgg gccgcggtta catcgacgac
     3061 gtgatcatgc ccgcggacac ccggccgcgc ttgatcgccg agctggagat gctcgccggc
     3121 aagcgccagg tcaacctgcc gcgcaagcac ggcaacatcc ccctgtaggc ggggcggagc
     3181 aagggtgtct ttgcacaaag acagcgcggg tcgggagggg cgctccaacg ccgcgccgat
     3241 ttcgccgttc gatcggctgc tgatcgccaa ccgcggcgag atcgcggtgc gcatcatccg
     3301 cgcctgccgc gagctgggca tc
//
LOCUS       DATBRX010000116         7453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1935647, whole genome shotgun sequence.
ACCESSION   DATBRX010000116 DATBRX010000000
VERSION     DATBRX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935647"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VIF08_04095"
     CDS             complement(<1..735)
                     /locus_tag="VIF08_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8135198.1"
                     /translation="MRTRTNLAFSSLLAALLLVAAVTPAAATTTTQADLAPEVRTWLA
                     TASTSAKLPVIVSFKNTTGLDRLSNLLGGVEHLDSVPMAYAKLSAGQIRTVASWSDTR
                     SVFGNRTYKLLDDVANRQIGADKVQTGYRLKRAYNGAGVGVAVVDSGVDATHPDLPYG
                     TKVKKNFYVLGDPLSGDEPSVFVEGSPNTDTEVGHGTHVAGTIAGIGAASGGKWVGVA
                     PGADIYMFRGGAGLNIFSWWAVRAFDW"
     gene            973..2856
                     /locus_tag="VIF08_04100"
     CDS             973..2856
                     /locus_tag="VIF08_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8135199.1"
                     /translation="MRPETRQLPRLSSRRIGAIALAVALLISAIIPSATTAASATPTG
                     LSQILAVNAPTTLARGIATFSAVPTAGQVNALKGLGLTVQPMHKVRLALVQGPVSAMQ
                     LAVTNGVALDVYPDNPIELLDTKSADAMGAAAVRAAGFTGKGVTVGVVDSGCDATHVD
                     LADHVVHNVTLSSAEYVNGDPNADNTIIVPVDQGPYSNTDVGGGHGTHVAGIIAADST
                     SAADGSHLGVAPDASLVCFAIGLVLFTSAVVTAYDVMMRQPDMWGIKVVNNSWGNSFR
                     QFDPNDPVHVVTKAVTELGVTVVFAAGNSGNDEMSLNPFSEAPWVISTGSGTLAHALS
                     DFSSTGLVYDNSEGGYIGAGGHTVYTGDRIGVYHPDVVGPGSSISSSCTSTGTVIAPC
                     IANGNGTASGTSMAAPHIAGAVAVLLQANPNLTPTQVRQVLQGTAKPILDANGKPLPF
                     WQVGYGYVDLNAAVAMVRSSSWKTKLASANTRANTRVLDQDGFKVSRSEFWTYAPPRA
                     TVAGTTDAHTYRFNVSSTTRYLKVTLSHPSTTVLIGNNMFYDVTVMDGAGSVIATGEE
                     LASAGTNTAFIDLRSVSGIKYGVFTVEISGFLAVSDPDTFDSDSLLGRMNTLQVAWVN
                     AGK"
     gene            complement(2915..4129)
                     /locus_tag="VIF08_04105"
     CDS             complement(2915..4129)
                     /locus_tag="VIF08_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEY8135200.1"
                     /translation="MIETVRQFRSWFWKPPRAHGEILADRRVSNLELLYDLVYVAVIS
                     QVAGALAEDVSIGGLIGFSVVFGLIWIGWVNGSLYLELHGQEDGRTRSYVFVQMGILA
                     VLAVFARDATHATGAQFALTYAAFLGVIAWLFFTVRRVDAPEIARVTGLYALTLGASA
                     LAIAVSAFLPEGARETIWAGFGVVWIGATILLGFRTRSFTIGVTPTDSMVERFNLFTI
                     IVLGEVVVGVVEGLSHVSGDLFAVATGILALFVGLGFWWIYFDIVGDRKPRLDGRALT
                     VWLLSHLPLTGAIAASGAAMVTLIEHANDPVAPQGPAWLLAVGTALVLLAEILCASAL
                     ADYDRMPGIRRTLGLALLIAAFVAIGVGLVQPPPWLLAVGLGMVLLLVWLVAVSKFMR
                     VSAWPPSRAQTE"
     gene            complement(4163..6820)
                     /locus_tag="VIF08_04110"
     CDS             complement(4163..6820)
                     /locus_tag="VIF08_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8135201.1"
                     /translation="MINQPAPGAETWIGISESARYLSVPMRTMYRLAQRGQVPAVKVG
                     RSWRFKRSALDAHLGGHRQQENTPAQTPAAEAMTDTARRLSDAHRWSQQLERIDALSR
                     RLNRSRDVAAVADAVASEIATVIDWHGLRFFLVEPDGIMRVIALRSTVSHYANEKPEM
                     LQIRVGQGLAGSVAATRVAEIVSDVRADKRGFKIPGTDDVDESMILVPLVYEDEVLGV
                     LSLTRLGRHAFDSTDLRLAQIIGAQAAVALSNARQLEELRRRGEALERRLASQRQLLA
                     ITERLLLTRDRTAIFDAIADTLAGVVPYDTLTIYLVDRQEHCLVPILARDPYAEQILA
                     TRPELGAGITGDVIERGEAEIINDAANDPRVIQVPGTPSDEQESLIVVPVLGKDSVVG
                     SLNLYRVARHFDEEDLELVKLFANHVAIALENATMNEQLVGAALTDPLTGLPNRRLFA
                     DRIDHALARRERTGAHVAVLFMDLDRFKLVNDGLGHAAGDAVLRAVGERLRAILRQSD
                     TVARLGGDEFGILVDEVAGIEEAGHTAQRVFDALAEPLLFDGRSVSVRASIGLAIDRE
                     IDTTSADELLRDADTAMYRAKANGRGCLAVFEPQMLAHQLARLELDVELREALLKDQF
                     EMRYQPIVDLTTGRIEEVEALVRWNHPTRGVLGPEAFIRASEETGHIVDLGAWVLREA
                     CERARGWLDKKLVSADFRVSVNTSVRQVVEAGFAGGVLGALAESKLAPHNLTIEITES
                     MLLADETASIAALHELRNAGVHIVIDDFGTGYSALSYLNQLPVDGLKIDRSFVLGLGR
                     EREKGAIVRATIAFARGLGLVVTAEGIETEAQLRQLVGLHCERGQGFLFSVPLSAGAL
                     SSLLRSHRTYELPSAHPSNVPRRSKVTVA"
     gene            7065..7268
                     /locus_tag="VIF08_04115"
     CDS             7065..7268
                     /locus_tag="VIF08_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEY8135202.1"
                     /translation="MATGTIKKVVADRGFGFITADDAKEYFFHRNSLDSSIDFDRLNG
                     GEKVEFEIEQSPKGPRANRVRSA"
BASE COUNT         1277 a         2582 c         2415 g         1179 t
ORIGIN      
        1 ccagtcgaac gcgcgcactg cccaccacga gaagatgttc agaccggcgc caccgcggaa
       61 catgtagatg tccgcgccgg gggcgacgcc gacccacttg ccaccgctcg cggcgccaat
      121 gccggcgatc gtgcccgcga catgtgtgcc gtggccgacc tccgtgtccg tgttggggct
      181 gccctcgacg aacactgatg gctcgtcgcc cgacagcggg tcacccagaa cgtagaagtt
      241 tttcttgacc ttggtgccgt acggcagatc agggtgggtc gcgtcgacac ccgagtcgac
      301 cacggccacg ccgacgcccg cgccgttgta ggcgcgcttc aacctgtacc cggtctggac
      361 cttgtctgcg ccgatctgcc tgttggcgac gtcatcgagc agcttgtagg tgcggttgcc
      421 gaagacgctc ctcgtgtccg accacgaggc cacggtccgg atctggccgg ccgacagctt
      481 cgcgtaggcc atggggacgc tgtcgaggtg ctccacgcca ccaagcagat tgctcagacg
      541 atccaggccg gtcgtgttct tgaacgagac gatcaccggc agcttggccg acgtgcttgc
      601 cgtcgccagc cacgtgcgta cctccggcgc cagatcggcc tgggtggtcg tcgtcgcagc
      661 ggcgggggtg accgcagcga cgagcagcag ggcggccagc agggagctga acgcgaggtt
      721 cgtgcgagtc cgcatttcca gtgtctcctt gcctactagg gcgggttcac aggcgggata
      781 ccactggtcg tggtgtcgcc accgcggcca cgataacgca tgccacaact caatgtggtg
      841 tcagacgtat aacaaatgcc ctccaggcct cccgttggct tgacaccatg ttgcgtggtg
      901 catgaccatc ggcggcgaac caccacccgc aagggtggcg catgtgggaa tccgaagcga
      961 aagaggagca cgatgcgccc cgaaacccgt cagctgccgc gcttgagctc gcgccggatt
     1021 ggtgcgatcg ctctcgcggt tgccctgttg atcagcgcaa tcatccccag cgccacgacc
     1081 gccgcgtcgg caaccccgac gggcctgtcg cagatcctgg ctgtgaacgc gccaaccacg
     1141 ctggcccgtg gcatcgccac gttctcggcc gtgcccacgg ccggccaggt caacgcgctc
     1201 aagggtctcg gcctgaccgt ccagcccatg cacaaggtgc gcctggcgct ggtgcaaggc
     1261 ccggtcagcg cgatgcagct cgccgtcacc aacggcgtcg ctctcgatgt ctatcccgac
     1321 aatcccatcg agctgctcga caccaagtcg gctgacgcga tgggcgcggc cgctgtccgc
     1381 gcggccggct tcaccggcaa gggcgtgacc gtcggcgtcg tcgactcagg ctgcgatgcc
     1441 acgcacgtgg acctcgccga tcacgtcgtt cacaacgtga ccctgtcgag cgccgagtat
     1501 gtgaatggcg acccgaacgc cgacaacacg atcatcgtgc cggtcgacca gggcccgtac
     1561 agcaacacgg acgtcggcgg cggtcacggc acgcacgtgg ccgggatcat cgcggccgac
     1621 tcgacgtccg cggccgacgg cagccacctt ggcgtggccc cggatgcgag cctcgtgtgc
     1681 ttcgcgatcg gcctcgtgct cttcacgtca gccgtggtca cggcctacga cgtgatgatg
     1741 cgccagcccg acatgtgggg catcaaggtc gtcaacaact cgtggggcaa ctcgttccgc
     1801 cagttcgacc ccaacgaccc ggtccatgtc gtgaccaagg cggtcacgga gctgggcgtc
     1861 acggtcgttt tcgccgccgg caacagcggc aacgatgaga tgagcctcaa cccgttctca
     1921 gaggcgccgt gggtcatttc gaccggctcg ggcacgctcg cccatgccct gtcggacttc
     1981 agctcgacag gcctcgtcta tgacaacagc gaaggcggct acatcggtgc cggcggccac
     2041 acggtttaca ccggtgaccg gatcggcgtg taccaccccg acgtggtcgg cccgggcagc
     2101 tcgatctctt ccagctgcac ctcgaccggc accgtcatcg cgccctgcat cgccaacggc
     2161 aacggaacgg cgtcaggcac gtccatggcc gcgccgcata tcgccggcgc cgtcgcagtc
     2221 ctgctgcagg ccaatcccaa tctgaccccg acgcaggtcc gccaggtcct ccaggggacg
     2281 gccaagccga tcctcgacgc caatggcaag ccgctgccct tctggcaggt tggctacggc
     2341 tacgtcgatc tgaacgccgc cgtcgccatg gtccgctcgt cgagctggaa gaccaagctc
     2401 gccagcgcca acaccagggc caacactcgc gtgctcgatc aggatggttt caaggtgagc
     2461 cgctctgagt tctggaccta cgcgccgccg cgggccacgg tcgcgggcac gacagatgcc
     2521 cacacctatc ggttcaacgt gtcctcgacg acgcgctacc tgaaggtcac gctgtcgcac
     2581 ccctcgacca ccgtgctcat cggcaacaac atgttctatg acgtgacggt catggacggt
     2641 gcgggcagcg tgatcgcgac aggcgaggag cttgcctcag cgggcacgaa cactgcattc
     2701 atcgacctgc gctcggtcag cggcatcaag tacggggtgt tcacggtcga gatcagcggc
     2761 ttcctcgccg tatcggaccc cgacaccttc gacagcgact cgctgctcgg ccgcatgaac
     2821 acgctccagg ttgcctgggt gaacgccggc aagtagcccc tcgtaaatcc ctctcccaag
     2881 agccgccgga accccacagc ccggcggctc ttttctattc cgtctgtgcg cgactgggcg
     2941 gccacgcgct gacgcgcatg aacttgctca ccgcgaccag ccagacgagc agcaggacca
     3001 tgcccaagcc caccgcgagc agccacggcg gcggctggac caggccgacg ccaatcgcca
     3061 cgaacgcggc gatcaacagc gccaggccca gggtgcggcg gatgccgggc atgcggtcgt
     3121 agtcggccag cgcggaagcg cacaggatct ccgccagcag cacgagcgcc gtcccaaccg
     3181 ccagcagcca cgccggtccc tggggagcga ccggatcatt ggcgtgctcg atcagggtca
     3241 ccatcgcggc gccagaagcg gcgatagcac cggtcagtgg gaggtgcgac aggagccaga
     3301 cggtgagtgc ccggccgtca agtcggggct tgcggtcgcc gacgatgtcg aagtagatcc
     3361 accagaagcc caggcccacg aacagcgcca ggatcccggt ggcgactgcg aacaggtcgc
     3421 cggagacgtg cgacaacccc tcgacgacgc ccaccacgac ctcgcccagg acgatgatcg
     3481 tgaagaggtt gaagcgctcg accatcgagt cggtcggcgt gacgccaatc gtgaaggagc
     3541 gcgtccggaa gccgagcagg atcgtcgcgc cgatccacac cacaccgaag ccggcccaga
     3601 tcgtctcgcg cgcgccctcc ggcaggaacg cggaaacggc gatcgccagc gcggacgcgc
     3661 cgagggtcag cgcgtagaga ccggtgacgc gggcgatctc cggcgcgtcc acgcgcctga
     3721 ccgtgaagaa gagccacgcg atgaccccca ggaacgcggc gtaggtcagc gcgaactgcg
     3781 cgccggtcgc gtgcgtcgcg tccctggcga acaccgcgag caccgcgagg atgcccatct
     3841 gcacgaacac gtaggagcgc gtgcgcccat cttcctggcc gtgcagctcc aggtagagcg
     3901 agccgttgac ccagccgatc cagatcagcc cgaagacgac gctgaagccg atcaggccac
     3961 cgatcgaaac gtcttccgcc aacgctccgg ccacctggct gatgacggcc acgtagacca
     4021 ggtcgtagag cagctcgagg ttgctgacgc gccggtcggc caggatctcg ccgtgcgccc
     4081 gcggcggctt ccagaaccag ctccggaatt gccgcaccgt ctcgatcatg gcgctgattg
     4141 tggcgccggc gacaaccgga tttcaggcga ccgtgacttt gctccgccgc gggacgttgc
     4201 tggggtgcgc gctcggcagt tcgtacgtcc gatgtgatcg cagcagactc gagagcgcgc
     4261 cggcgctgag cggcacggag aacaggaagc cctggccgcg ctcgcaatgc agaccgacga
     4321 gctgccgcag ctgcgcctcc gtctcgatgc cttccgcggt gaccacgagc ccaaggccgc
     4381 gcgcgaacgc gatggtcgcg cgcacgatcg cccccttctc gcgctcgcga cccaggccca
     4441 gcacgaacga gcggtcgatc ttgagcccgt cgaccggcag ctggttgagg tagctcaggg
     4501 cggagtagcc ggtgccgaag tcgtcgatca cgatgtgcac accggcgttg cgcagctcgt
     4561 ggagcgcggc gatggacgcc gtctcgtcgg ccaacagcat gctctccgtg atctcgatgg
     4621 tcaggttgtg gggcgcaagc ttgctctccg ccagggcacc aagcacgccg ccggcgaatc
     4681 cggcctccac cacctggcgc actgacgtgt tgacagacac gcggaagtca gcgctgacca
     4741 gcttcttgtc gagccagccc cgggcgcgct cacacgcctc gcgcagcacc caggcgccca
     4801 gatcgacgat atggcccgtc tcctcgctcg ccctgatgaa agcctccggc cctagaacgc
     4861 cgcgcgtcgg atggttccag cgcaccagcg cctcgacctc ctcgattcgc ccggtggtca
     4921 ggtccacgat gggctggtaa cgcatctcga actggtcctt caagagcgcc tcgcgcaact
     4981 cgacgtcaag ctccaggcgc gccagctgat gggcaagcat ctgcggctcg aacaccgcca
     5041 ggcagccccg tccgttggcc ttggcgcggt acatggccgt gtccgcgtcg cgcagcagct
     5101 catcggcact ggtcgtgtcg atctcgcggt cgatggcgag gccaatgctc gcccgcacgg
     5161 acacgctccg gccgtcaaac aggagcggtt cggccagcgc atcgaagacg cgttgcgcgg
     5221 tgtggcccgc ttcctcgata cccgccacct catcgaccag gatgccgaac tcgtcgccac
     5281 ccaggcgggc cacggtgtcg ctctggcgca ggatggcgcg caatcgctca cccaccgcgc
     5341 gcagcaccgc atcgcccgct gcatggccga gtccgtcgtt gaccagcttg aaccggtcga
     5401 gatccatgaa caggaccgcg acgtgggcgc cggtccgctc gcgccgtgca agggcatgat
     5461 cgattcggtc cgcgaagagg cgtcggttgg gcagcccggt cagcggatcg gtcagcgctg
     5521 cgccaacgag ctgctcgttc atcgtcgcgt tctcgagcgc gatggccacg tggttggcga
     5581 acagcttgac gagctccagg tcctcctcgt cgaagtgccg cgcgacccgg tacaggttga
     5641 gcgaaccaac gacgctgtcc ttgcccagga ccgggacgac gatgagcgac tcctgctcgt
     5701 cgcttggcgt gcccggcacc tggatgacgc gcggatcgtt ggcggcgtcg ttgatgatct
     5761 cggcctcgcc gcgctcgatg acgtcgccgg tgatgcctgc ccccagctcg ggccgcgtcg
     5821 ccaggatctg ctctgcatag gggtcgcgcg cgaggatcgg cacgaggcag tgctcctggc
     5881 gatccacgag atagatggtc agcgtgtcat acggcacgac cccggccagc gtgtcggcga
     5941 tggcatcgaa gatcgcggtc cggtcgcgcg tcagcaggag ccgctcggtg atggccagca
     6001 gctgacgctg gctggccagc cgtcgctcca gcgcttcacc gcgccggcgc agctcctcga
     6061 gctggcgcgc gttcgacaag gcgacggcgg cctgcgcgcc aatgatctgg gccaggcgca
     6121 ggtcggtcga gtcgaaggca tgccgtccga gtcgggtgag tgagagaacg cccaacacct
     6181 cgtcttcgta gacgagcggc accaggatca tcgactcgtc gacatcgtcc gtgccgggga
     6241 tcttgaagcc acgcttgtcg gcccgaacat cggacacgat ctcggccacg cgcgtcgcgg
     6301 ccacgctgcc ggcgagaccc tggccaacgc ggatctggag catctctggc ttctcattgg
     6361 cgtagtgcga gaccgtgctg cgcagggcaa tgacgcgcat gatcccgtcc ggctccacca
     6421 ggaagaagcg caggccgtgc cagtcgatga cggtcgcgat ctcactcgcg acggcgtcgg
     6481 ccaccgcggc aacgtcgcgc gacctgttga ggcgccgcga cagggcatcg atgcgctcca
     6541 gctgctggct ccagcggtga gcgtcggata gccgccgcgc cgtgtcggtc atcgcctccg
     6601 cggccggcgt ctgcgcgggc gtgttctcct gctgacggtg gccaccaagg tgagcgtcta
     6661 gcgctgaacg tttgaaacgc cagcttcggc cgaccttgac cgccggcacc tggccgcgct
     6721 gggcaagccg atacatcgtc cgcataggta cggacagata gcgcgccgat tcggagatcc
     6781 cgatccaggt ttcggccccg ggcgctggct gattgatcaa gacgcgcaat tgccccttgc
     6841 tgccacttgt tgctcggtcg tgaccgtgag tctccctgcc agcgcagcgg ccccggaacg
     6901 gtccggtagt cccgtggtgc cacccgaaag tgccaattca cccagtggcg agccaattcg
     6961 gggtagaatg tctgctcacc gcgatgtgcg gtggcctcgt gtgatcgacc ctctcgaacg
     7021 gatcgtgcgc agtgttcccg gttcgagaat gtttggaggt caatgtggcc accggtacca
     7081 tcaagaaggt cgtcgccgat cgcggcttcg gcttcatcac tgcggatgac gcgaaggaat
     7141 acttctttca ccgcaactcg ctcgactcct cgatcgactt cgatcgcctc aatggcggcg
     7201 agaaggtcga gttcgagatc gagcagagcc ccaaggggcc ccgggcgaac cgagttcgct
     7261 ccgcctaagc gctagtcgct aaagcgaagg tcgcccgcag caatgcgggc ggccttttga
     7321 ttccggacgc ggccgacggt cagttggtga accagggcgc gagcgcgacc ttgatcgtcg
     7381 cgaagggcgg cgcggcccaa tcgatcgcga cctggcagcc ctcgatgtgt tccacgccat
     7441 ctggcgtgct gaa
//
LOCUS       DATBRX010000117         8969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3500467, whole genome shotgun sequence.
ACCESSION   DATBRX010000117 DATBRX010000000
VERSION     DATBRX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3500467"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VIF08_04120"
     CDS             complement(<1..263)
                     /locus_tag="VIF08_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135203.1"
                     /translation="MGVYLTRGQPSALALVRRAIESERPPHALLLVGPTGVGKTTLAM
                     DLAAGLLCLAADPADRPCGQCAACHKVAHGNHPDLHLLAPEGAG"
     gene            complement(274..2103)
                     /locus_tag="VIF08_04125"
     CDS             complement(274..2103)
                     /locus_tag="VIF08_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY8135204.1"
                     /translation="MSKNVAVFVDVANIFYAAKAAGVDIDYVTLLKAATAGRDFVRAY
                     AYTGLDPENDNQRQFHSFLARSGYKVISKDIRKYGDGRIKANLDIELVVDLMKTARNL
                     DIAVVVSGDGDFASAIRAVQEMGVRVEVISFRGNTSSDLIDVADQFTDIAQVARVEKG
                     SARSGRRVAAEGDLSMTEVPEKETEGAPRRRRGRAAEERQRTPVAAAPVATEAVPAAN
                     LVALPGERLSRGAGAGDDAEGEGADDALDAAVDGDADGDGLTEDGRRRRRRRGGRGRG
                     RGRRTDEAGMPLEPAAAVAGGPAAGEEDEDFDEPQPANLPHHTFGSVWDSQIGLPTSG
                     SPAVDSLSGEMSDEEDLEEPEVPEYLLAERRQRGGARQGGRPGARPARGRSAYQAALD
                     RERFGNRGAPQPSFGGTSTRSPGGNRDRGRGGRPTGQGGSRPPRAPRMDDRPRYDEAR
                     SSSEPWSEVPPELEEMLRAELSRKAPAATTGDRPVSQAQISEVPLSEMPAPIASFGAT
                     TAADAPAAAKPARRTRTTKAKPADSSSAGEAATAATSAQAGAPAARSRTTKAAATKST
                     ATRTRKAASAADGEAAPKKPATRRKSAAAAEPAAESTSEATEG"
     gene            complement(2166..2624)
                     /locus_tag="VIF08_04130"
     CDS             complement(2166..2624)
                     /locus_tag="VIF08_04130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135205.1"
                     /translation="MDLQVREARLTDIDRITGLIERTDSRWTLSRLADAADVLRQMIY
                     LPNGSLIVALDGRMVVGTAVLALRPSVSAGGLVGSIDLLAVEPGHELDGVVEALLGET
                     IRTARNKGCVVLESEPPEDPAMLTRWEAAGFSEAGSRLRCPLARAAALTW"
     gene            3148..4362
                     /locus_tag="VIF08_04135"
     CDS             3148..4362
                     /locus_tag="VIF08_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8135206.1"
                     /translation="MTAAPSGGPSITAVKIGVDLPLSGASVANGQPTLQGIELAVKEW
                     NDAAMGHAYTVELNVQDDAVNGVYNPEQGASNANTLVADDEVVGMVGPFNSSVARAII
                     PVTNEFGLAQCSPANTAVDLTKEGSEVYRPAKPDQRNYFRVATPDDVQGPAGAQYTYN
                     DLGKRSVLVIDDTTAFGVGVADSYSTEFEALGGTIVDRVSNDYAVDTSFSADLTAAGD
                     AFDVVFFGGTQTLGGGQLRRDMGAAGLLNIPLVGPDGITDLGKGGDEGTFITLAGVEN
                     SDNVFGTIAGANALELDIAADFNAAYEAEYGVTPGAYSALAHACATVLLQAIDANIGT
                     AADLAALREAVRASIIGQDTLWSTVLGELSFDANGDSSQKWISFYRTDPSLNGGAGGW
                     VFDKQQDFSVTE"
     gene            4430..5365
                     /locus_tag="VIF08_04140"
     CDS             4430..5365
                     /locus_tag="VIF08_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8135207.1"
                     /translation="MIDDALIATLTQQFFNALAIGGIYALIALGYTMVYGIIELINFA
                     HGEIFMVGAFFGLTFLTAIGFTGAIANPITFLVVLLAAFAVVMPLLGLVGIGIERFAY
                     RPLRNAPRLAPLITAIGVSFILQNIVYIWHGPSPVQFPQILSPQVRFSILGASVSYIS
                     VLIVAVTAVLLVALQLFIGRSRLGRAMRSTAQDRDAAQLMGVNINTTIALTFFIGSAL
                     AGAAGIVYGLYFGTIAFNMGFIGGLKAFTAAVLGGIGNTTGAVLGGFMIGFIEVLSAQ
                     LGLAIWAHAIVFGVLVIVLVFRPAGLLGQPIGERA"
     gene            5362..6573
                     /locus_tag="VIF08_04145"
     CDS             5362..6573
                     /locus_tag="VIF08_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135208.1"
                     /translation="MTAVAPAKNAPRRPALRSARDYLDRHRSLSIIIGMAIVTAILPF
                     IVTLPPFSGFDAQRVWIGSFATAGIFVLLAIGLNVVVGLSGLLDLGYAAFYAIGAYTY
                     AFAASSFTGIHIPFWPMLLVGALIAAIFGILLGAPTLRLRGDYLAIVTLGFGEIVPIL
                     FRNADARNIGVNLTNGTNGIGGIDRPTLPVIQIGDFKILDVVFNAANPLSYYLTMAII
                     ITLVLILVYRLQDSRLGRAWQAIREDELAAASNGVNTITTKLLAFALGATTSGFAGVF
                     SASKLVFVSPDQFGFTVSFTILAMVVLGGMGNSIGVALGAFIIYVIQSVFLKQMTNFF
                     DDFQVPILSDINFIEYQFLLYGIALVAMMLLRPEGLFPSRQRARELHTDEGLDPMEGS
                     PSGGDAEGSLA"
     gene            6570..7679
                     /locus_tag="VIF08_04150"
     CDS             6570..7679
                     /locus_tag="VIF08_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135209.1"
                     /translation="MTDDATAGSAPAADDTAAGDISGDDPKAVLAGAWADGADTSDIM
                     LRARSVTKRFGGLVAVREADLVIPRGKIVSLIGPNGAGKTTFFNIVAGILDPTAGDIE
                     FRGRKMIARPVRAWFEPVLWFVPSLIIVAVTWLLTAFGAGVAALEVGILLSVLALVVT
                     MLLAIVRPVWYSRLLTRLGVFKSARPNDMVEAGIGRTFQNIRLFQNMSAIENVLVGMH
                     TRLKSNMVDAMLSTPRRNREERDGRKRARELLALVGLRGRDHALARNLPYGDQRRLEV
                     ARALASNPVLLLLDEPTAGMNPAETAEMTRLISRLRVELGLTILLIEHDMRVVMGISD
                     RITVLDHGEQIAEGTPDEVRRDPKVIEAYLGAPSS"
     gene            7688..8431
                     /locus_tag="VIF08_04155"
     CDS             7688..8431
                     /locus_tag="VIF08_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135210.1"
                     /translation="MPATLASTAPDGAFLTLTKVNSYYGRIRALQDLSLEVGKGEIVT
                     LIGANGAGKTTTLRTISGLLHARDGSVSFDGEDISHTPAHELVRRGIGHAPEGRRIFS
                     RLTVMENLQMGGFTRTAAERKEDLDRALTLFPRLKERLNQKGGTLSGGEQQMLAIGRA
                     LMARPRLLMLDEPSLGLSPILVQQIFDIIREINAQGTTVLLVEQNALQALAVAHRGYV
                     LQTGTVILADTAAALAANEDVRRVYLGEI"
     gene            8466..>8969
                     /locus_tag="VIF08_04160"
     CDS             8466..>8969
                     /locus_tag="VIF08_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135211.1"
                     /translation="MPLERIRGQAVGRREIAILVATVLAIGLFVLKPWDEPPPLTALA
                     PTPEPRPTVSDSAPPPVVPTPRPTADAAMLAERRRFCQPAETWRLVSIEETARWRIRT
                     MWAGTPQRAAGPGDVSLVARRDNAERLMAVGVCAPTDPIVALPDQLARVTLWHVPTDG
                     PPRAISGP"
BASE COUNT         1379 a         3115 c         2921 g         1554 t
ORIGIN      
        1 ccggcaccct ccggcgccaa cagatgcagg tcggggtgat tgccgtgggc caccttgtgg
       61 cacgcggcgc attgcccgca cggccgatcg gccgggtcag ccgcaagaca cagcagcccc
      121 gcggccagat ccatggcgag cgtcgtcttg ccgacacccg tcgggccgac gagcagcagc
      181 gcgtgcggcg gccgctcgct ttcgatcgcg cgccggacca acgccagcgc tgacggttgg
      241 ccgcgggtca ggtacacgcc cacgtccccg ggctcagccc tccgtggctt ccgacgtgga
      301 ctcggcagcc ggctcagctg cggcagcgct tttgcgccgc gtggccggct tcttgggcgc
      361 agcctcacca tcagccgctg acgccgcctt gcgggtccgc gtcgccgtcg acttggtggc
      421 ggcggccttg gtcgtgcgcg accgcgccgc cggggcgcca gcctgggccg acgtcgcagc
      481 cgttgccgcc tcaccggccg atgaactgtc agccggtttg gccttggtgg ttcgggttcg
      541 acgggcaggc ttggccgccg cgggggcatc ggccgctgtc gttgctccga aggaagctat
      601 tggcgccggc atctcgctca gcggcacttc gctgatttgc gcctggctta ccggcctatc
      661 gcccgtggtc gcggcgggcg ccttgcgcga cagctcggca cggagcatct cctcgagctc
      721 cggtggcacc tccgaccacg gctcgctcga ggaacgtgcc tcgtcgtagc gcggccggtc
      781 gtccatgcgc ggagcgcgcg gtggccggct gccaccctgg cccgtgggcc ggccaccgcg
      841 accacggtca cgattgccac ccgggctgcg cgtgctcgtg ccgccgaaag acggctgcgg
      901 cgcgccgcgg tttccgaagc gctcgcggtc gagtgccgcc tggtaggcgg atcggccgcg
      961 cgccgggcgg gcgccggggc gaccaccctg acgcgcaccg ccgcgttggc gacgctcggc
     1021 cagcaggtac tccggcacct cgggctcctc gaggtcctcc tcgtcggaca tctcgccgct
     1081 cagcgagtcg accgcaggcg atcctgaagt cggcaggcca atctgactgt cccagaccga
     1141 gccaaaggtg tgatgcggca ggttcgcggg ctggggctcg tcgaagtctt cgtcttcctc
     1201 cccagcggcg ggcccaccgg cgacagctgc ggctggctcg agcggcattc ctgcctcgtc
     1261 ggtgcgccgg ccacggccgc gaccgcggcc accgcggcgg cgtcggcggc ggcgtccgtc
     1321 ctcggtcagg ccatcgccat cggcatcgcc atcgacggca gcgtcgagcg cgtcatccgc
     1381 accctcgccc tcggcgtcgt cgcctgcgcc cgcaccgcgc gaaaggcgct cgccgggcag
     1441 cgcgaccagg ttcgccgcag gtacagcctc agtagcgacg ggagccgccg cgaccggcgt
     1501 gcgctggcgc tcctcggccg ctcgcccgcg gcggcggcgc ggcgcgccct ctgtttcctt
     1561 ctcgggcacc tcggtcatcg acaggtcgcc ctcggcggcc acgcggcgac cgctgcgcgc
     1621 tgagcccttc tcgacgcgcg ccacctgggc gatgtcggtg aactggtcgg ccacgtcgat
     1681 caggtcagac gacgtgttgc cgcggaagct aatgacctca acgcgcacgc ccatctcctg
     1741 gacggcacgg atggcgctcg cgaagtcgcc gtcgcccgac acgaccacgg cgatgtcgag
     1801 gttgcgcgcc gtcttcatca ggtcaaccac gagctcaatg tcgaggttcg ccttgatgcg
     1861 gccatcgccg tacttgcgga tgtccttgct gatgaccttg tagccgctcc gcgcgaggaa
     1921 cgagtggaac tggcgctggt tgtcgttttc cgggtcgagc ccggtgtacg cgtacgcgcg
     1981 cacgaaatcg cgccccgcgg tggccgcctt gagcagcgtg acgtagtcga tatcgactcc
     2041 ggctgccttg gcggcataga agatgttcgc cacgtctacg aagacggcga cgttcttact
     2101 cacgaagttc tcctgttttg ttgtgcgcgc cggttacggc gcggtggccg cgtgtgcgcg
     2161 gccgattacc aggtcagcgc cgcggcccgc gcgagcgggc agcgcagacg cgaaccggct
     2221 tcgctgaagc ccgccgcttc ccagcgcgtc aacatggcgg ggtcttcggg cggctcactc
     2281 tcgagcacga cacagccttt gttgcgcgcc gtgcggatcg tctcgccgag caatgcttcg
     2341 accacgccat cgagctcgtg tccgggctca accgcgagaa ggtcgatgct gcccaccagg
     2401 ccgccggcgc taaccgaggg ccgcagcgcg aggaccgccg tcccgacgac cattcggcca
     2461 tcaagcgcca cgatcaacga tccgttcggc aggtagatca tttgacgcag gacatcggcc
     2521 gcgtcagcca agcggctgag cgtccagcgt gaatcggtgc gctcgatcag accggtgatc
     2581 cgatcaatgt cggtcagccg cgcttcgcgt acctggagat ccacgctcgc caaggtccct
     2641 cgtctggctc aggcgccccg cgtgggtgcc cgaaacgtct catcaaaccg gtagatccgg
     2701 ccgccgctcg agcgcgccgc tccgggacgt gcagggtgcc accttgcggc ggccctgtcg
     2761 attcgctccg gggcgcattc aaacgtctgt tgcaaacgag acgtcgcggc ctatcatacg
     2821 gcatcgcgtc agcgggtcag ccgtctgcgc cctttgtgta cggtcacctt ttagggtcga
     2881 ggcgcggcca atcgagcggt tccgctaaac caatccatta cggactcgat ggacgtcact
     2941 ctcccctact cggaccgacc gggccggaag gctccgcgcg atgcggagga ggacaagcaa
     3001 actttgagga ggaacctagg gcttatgaag ctccgatatt ccagcgcgct ggccgttacg
     3061 gcgatgatcg ccgtggcctg cggcacgggc ggcgcatcgc cgaccccgcg cgctagtgcc
     3121 accgcgggcg ctagtgctac tcccggtgtg actgcggcgc cttcgggcgg accgtcgatc
     3181 acggcggtca agatcggcgt cgacctgccg ctgtccggcg catccgtcgc caacggccag
     3241 ccaacccttc agggcatcga gctggcggtc aaggagtgga acgacgccgc aatgggccac
     3301 gcctatacgg tcgagctcaa cgtccaggac gacgccgtca acggcgtgta caacccggag
     3361 cagggcgcaa gcaatgccaa caccctcgtc gctgatgacg aggtcgtggg catggtcggc
     3421 ccgttcaact ccagcgtcgc gcgggcgatc atcccggtaa ccaacgagtt cgggctcgca
     3481 cagtgcagcc cggccaacac cgccgtcgac ctgaccaagg aagggtcgga ggtttaccgc
     3541 ccggccaagc ctgaccagcg caactacttc cgcgtcgcaa ccccggatga cgtccagggc
     3601 ccggcaggcg cgcagtacac gtacaacgac ctcggcaagc gctccgtgct ggtgatcgat
     3661 gacacgacgg ccttcggcgt cggcgtggcg gactcgtaca gcactgagtt cgaagcgctc
     3721 ggcggcacca tcgtcgaccg cgtcagcaat gactacgcgg tcgacaccag cttctcggcc
     3781 gacctgaccg cggcgggcga cgcgttcgac gtcgtcttct tcggcgggac ccagactttg
     3841 ggcggcggcc agctgcgccg cgacatgggc gcagccggcc tgctcaacat cccgctcgtc
     3901 ggcccggacg gcatcaccga cctgggcaag ggcggcgacg agggtacgtt catcaccctc
     3961 gccggggtcg agaactcaga caacgtgttc ggtaccatcg cgggcgccaa cgccctcgag
     4021 ctggacatcg cggccgattt caacgccgcc tatgaggcgg aatacggtgt gaccccgggt
     4081 gcgtacagcg ctctggcgca tgcctgcgca acggttcttc tgcaggccat cgacgccaat
     4141 atcggcacag cggcagacct cgcggcactc cgcgaagctg tccgcgcgtc gatcattggc
     4201 caagacaccc tctggagcac tgtgctcggc gaactctcgt ttgacgccaa cggcgactcc
     4261 agccagaagt ggatctcgtt ctaccgaacg gatccctcgc tcaatggcgg agcgggcggc
     4321 tgggtcttcg acaagcagca ggacttctcg gttaccgagt aatccggctt gaattgacag
     4381 ccggggagcc gcaaagctcc ccggctgtct tgttatcgcg agcgagacga tgatcgatga
     4441 cgccctgatc gcgaccctga cgcagcagtt cttcaacgcg ttggcgattg gcgggatcta
     4501 cgccttgatc gctctcggct acaccatggt ctacggcatc atcgagctca tcaacttcgc
     4561 ccacggcgag attttcatgg tcggcgcgtt cttcggcctg acgttcctga cagcgatcgg
     4621 cttcacgggt gccattgcca acccgatcac ttttctcgta gtgctgctgg cggcgttcgc
     4681 cgtcgtgatg ccgttgctcg gtcttgtcgg catcggcatc gaacggttcg cctaccgtcc
     4741 ccttcgcaac gcgccgcggc tcgcgccgct gatcacggcg atcggcgtgt cgttcatcct
     4801 gcagaacatc gtgtacatct ggcacggtcc gtcgccggtc caattcccgc aaatcctgag
     4861 cccgcaggtg cggttctcca tcctgggcgc gtccgtcagc tacatcagcg tcctcatcgt
     4921 cgccgtcacc gcggtactgc ttgtggcact gcagctgttc atcggccgta gccgtctcgg
     4981 ccgggcgatg cgctccactg cccaggatcg tgacgcggcc cagctcatgg gcgtcaacat
     5041 caacacgacg atcgcgctga ccttcttcat tggctcggcg ctggccggtg ccgctggaat
     5101 cgtctacggc ctgtacttcg ggacgatcgc cttcaacatg ggtttcatcg gaggcctgaa
     5161 ggcgtttact gcggccgtgc tgggtggtat cggaaacacg acgggagccg tgctcggcgg
     5221 cttcatgatc ggcttcatcg aggtcttgag cgcgcagctc ggcttggcga tctgggctca
     5281 cgcgatcgta ttcggtgtcc tcgtgatcgt cctcgtgttc cggccagcgg gcctactcgg
     5341 gcaaccgatc ggagagcgcg catgaccgct gtcgcgccgg ccaagaacgc cccgcgccgc
     5401 cccgcgctga gaagcgcacg ggactacctc gaccgtcatc gctcgctctc gatcatcatc
     5461 ggcatggcca tcgtgacggc gatcctgccc ttcatcgtga cgctgccccc gttcagtgga
     5521 ttcgatgccc agcgcgtgtg gatcggctcg ttcgccacgg ctgggatctt cgttctactc
     5581 gccatcgggc tgaacgtcgt ggtcggcctg tccggcctgc tcgaccttgg ctacgccgcg
     5641 ttctacgcca tcggcgccta cacctacgcg ttcgcggcgt cgagcttcac cgggatccac
     5701 atccccttct ggccgatgct cctcgtcggc gcactcatcg ccgcgatctt cggcatcctg
     5761 ctcggcgccc cgacgctgcg actcagaggc gactacctgg cgatcgttac cctcggcttc
     5821 ggcgagatcg tgcccatcct gttcaggaac gccgacgcgc gcaatatcgg cgtcaacctc
     5881 acgaacggca ccaacggcat tggcgggatc gatcggccga ctcttccggt aattcagatc
     5941 ggcgacttca agatcctcga cgtcgtcttc aacgcggcca atccgctcag ctactacctc
     6001 acgatggcga tcatcatcac gctcgtcctg atcctggtct accggcttca ggactcgcgt
     6061 ctcggccggg cgtggcaggc catccgagag gacgagttgg cggctgccag caacggcgtc
     6121 aacacgatca ccaccaagct cctggcgttc gccttgggcg ccacgacgtc agggttcgcc
     6181 ggcgttttct ccgcctcgaa gctcgtcttc gtgagccccg accagttcgg gttcaccgtc
     6241 tcattcacga ttttggcgat ggtcgtactg ggcggcatgg gcaacagtat cggcgtggcg
     6301 ctcggcgcat tcatcatcta cgtcatccaa tcggtgttct tgaagcagat gaccaacttc
     6361 ttcgacgact tccaggtgcc gattctctcg gacatcaact tcatcgagta ccagttcctg
     6421 ctgtacggca tcgccctcgt agcgatgatg ctgttacgac ctgagggcct cttcccgagt
     6481 agacaacgcg cgcgcgagct gcacacagac gaaggcctgg acccgatgga gggctcgccc
     6541 agcggcggcg acgcggaggg tagcctcgca tgactgacga cgcgacggcc ggctcggctc
     6601 cggccgccga cgacacggcc gcgggcgaca tttccggcga cgacccaaag gccgtcctcg
     6661 ccggcgcctg ggcggacggc gcggacacaa gcgacatcat gctgcgggca cgatctgtga
     6721 ccaagcgctt cggcggcctg gtcgccgtgc gcgaggcaga cctggtgatc ccgcgcggca
     6781 agatcgtgag cctgatcggg ccgaacggcg cgggcaagac gacgttcttc aacatcgttg
     6841 ccggcatcct cgacccgacc gcgggcgaca tcgagttccg cggacggaag atgatcgccc
     6901 gccccgttcg cgcctggttc gagcctgttc tgtggttcgt cccgtcgctc atcatcgtcg
     6961 ccgttacgtg gctgctcacg gcattcggcg ccggcgttgc ggcgctggag gtcgggatac
     7021 tactttctgt gttagcgctg gtcgtgacca tgctgctggc gatcgtgcgc ccggtctggt
     7081 actcgcggtt gttgacgcgc ctgggcgtgt tcaagagcgc acgaccgaac gatatggtcg
     7141 aggcaggcat cggccgaacg ttccagaaca tccgtctctt ccagaacatg agcgcgatcg
     7201 agaacgttct ggtgggtatg cacacccgcc tgaagtcgaa catggtcgac gccatgctgt
     7261 ccacgccgcg gcgcaaccgc gaggaacgcg acggccggaa gcgggcgcgg gagctcctgg
     7321 cgctggtcgg ccttcgcggg cgtgaccacg cactggcgcg caatctgccc tacggggacc
     7381 agcgccgtct cgaggtcgcc cgcgcgttgg cgagcaaccc cgtgctgctc ctcctcgacg
     7441 aaccgaccgc gggcatgaac cccgccgaga cggccgagat gactcggctg atcagccggc
     7501 tccgcgtcga gctgggcctg accatcctgc tgatcgagca cgacatgcgc gtggtcatgg
     7561 gcatcagcga tcgaatcacc gtccttgatc acggcgagca gatcgccgag ggcacgcccg
     7621 acgaggtgcg ccgcgatccc aaggtcatcg aggcctacct gggggcgccg tcatcgtgac
     7681 cgaagcgatg ccggccactc tcgccagcac ggcgcctgat ggcgcgttcc tgacgctgac
     7741 gaaggtgaat tcgtactacg gtcggatccg ggccctacag gacctcagtc ttgaggtcgg
     7801 caagggcgag atcgtcaccc tgattggcgc caacggcgcg ggcaagacga caaccctgcg
     7861 gacgatctcg ggtctgctcc atgcccgcga cggatcggtc tcgttcgacg gcgaggacat
     7921 cagccacaca cccgcgcatg agctcgtgcg gcgcggcatt ggccatgctc ccgagggccg
     7981 gcgcatcttc tcccgactga cggtgatgga gaacctgcag atgggcggct tcacgcgcac
     8041 ggcagccgag cgaaaagagg acctggaccg cgcgctgacg ctgtttccgc gcctcaagga
     8101 gcggctgaac cagaagggcg gcactctttc tgggggcgag cagcagatgc tcgcgatcgg
     8161 acgtgcgcta atggcgcggc cgcgtctcct gatgctcgac gagccctcgc ttggcctttc
     8221 cccgatcctg gtccagcaga tcttcgacat catcagggag atcaacgccc agggcacgac
     8281 tgtcctcctc gtcgaacaga acgcccttca ggcgctcgcc gtggcgcacc gtgggtacgt
     8341 cctccaaacg gggaccgtga tcttggcgga cactgcggct gccttggccg ccaacgagga
     8401 cgttcgacgt gtctacttgg gggagatcta gcgtcccacc gccaagggaa gacgcaccgc
     8461 tggtggtgcc gctggagcgt atccgcggcc aggcggtggg caggcgcgag atcgccattc
     8521 tcgtggcgac cgtgctggcg atcgggctgt tcgtcctgaa accgtgggat gagccgccgc
     8581 ctctgacggc cctcgcgccg acacctgaac cacggccgac ggtctccgac agcgccccac
     8641 cgccggtggt tccgactccg cgcccgaccg ccgatgcggc gatgctggcg gagcgacgcc
     8701 gcttctgtca gcccgctgag acgtggcgcc tggtgagcat cgaagagact gcgcgctggc
     8761 ggatacgcac gatgtgggcc gggacgccgc agcgcgcggc gggcccgggt gacgtcagcc
     8821 tggttgcgcg gcgcgacaac gccgagcgcc tgatggccgt gggtgtctgc gcaccaaccg
     8881 acccgattgt ggcgctgccc gaccagctgg cacgcgtcac cttgtggcac gttcccaccg
     8941 atgggccgcc gcgcgcgatc agcggtcca
//
LOCUS       DATBRX010000118        11597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3390400, whole genome shotgun sequence.
ACCESSION   DATBRX010000118 DATBRX010000000
VERSION     DATBRX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11597
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3390400"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..900)
                     /locus_tag="VIF08_04165"
     CDS             complement(310..900)
                     /locus_tag="VIF08_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8135212.1"
                     /translation="MRLSLIVNILLVVAVATTVIGVGLFTNLLAVPGGATATTTPPPN
                     TGPLPTFSPSPSPVSSETPTPAPSVRPSPGGTYEVQPGDNLTKIANLYGISVAALIEA
                     NPQLLPPNYVIQPGDVLTIPNPAAECDGYEAYSVQSGDFLVSIGEQFSVSPTDIADFN
                     GLPWAGRHDYSDIRPGDILCIPAPGWTPLRTPVPTP"
     gene            complement(913..1764)
                     /locus_tag="VIF08_04170"
     CDS             complement(913..1764)
                     /locus_tag="VIF08_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEY8135213.1"
                     /translation="MGLSGPPQIGVRAGLPEPADATGAPVSFLVDYDGTISRVDIGDE
                     LLARHYPDKAEIARKDAQYDAGEVGSRELMRWDMDVLPHDAELLRNEAARMPQDGGFP
                     GFVQAVRERGALVEVVSDGLGFYVRPNLAALGVADLPVATNDNTVADGGAGMSFPYSH
                     PACFVCGTCKRERVRLHQAAGRVVVFIGDGISDRYAAAHADVTFAIGALTRICAAEGW
                     PFYEWRDFADLRAQVGAMFDDGRLPRSLADMASWHAAHSQPPRPFICGPEVWGPGRIN
                     PPNGQSV"
     gene            complement(1781..1951)
                     /locus_tag="VIF08_04175"
     CDS             complement(1781..1951)
                     /locus_tag="VIF08_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135214.1"
                     /translation="MKRLTKLFVLLFVLLLAAVIVVPLVALAGLFIWLKMTEEADDEA
                     MDLEPDSTDGAS"
     gene            complement(2035..2532)
                     /locus_tag="VIF08_04180"
     CDS             complement(2035..2532)
                     /locus_tag="VIF08_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135215.1"
                     /translation="MRIYEGAPRQNYEDVLRSIGALLDQRGMREITLNETEDGFIIQG
                     LALVADEERPWNDPAARLHKETVVLREDDIARFVEEGVTRRRPPAGPPAPNAQAFYEP
                     ALRALGVYLDQQQPRDIFFFEQEQQFVVRLLMQTRAGLRHMIVEFTREDIGAMIAAGM
                     RARGR"
     gene            complement(2533..4080)
                     /locus_tag="VIF08_04185"
     CDS             complement(2533..4080)
                     /locus_tag="VIF08_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY8135216.1"
                     /translation="MNREFQPVQLGLSGRSERSGVAAAALSFIFPGLGQAYLRDRRAA
                     IIFAAPIVVLLAVLLAFVSLDGVNRVGAKLLNPSIALGAALLALLVGVWWIINVINAW
                     RGGRHTGTNAFIAPAILIVALAAGTLYGADWMYRLSLADRNFGTGCDPTLDCPPGPTP
                     SGIAVVPTTPPPSGPTFPPEDTPEPSEPPATIAPGPSPSFDITKLSLEDGWLNVLLVG
                     IDSRCAGVGVVTGANTDTMIVASANADTGEVFMFSFPRDSAGFPLYVGGSMPGYWKLN
                     TFTGYTKQHPEVFPEPGLPALAYELGYLLGIPIDYYASINICGFPHLIDEVGGVDICN
                     SKLIQDSGYHWTDGHVGFTLETGPHHLDGETALAYARSRHGSSDFARARRQQELLGAL
                     RKAVLTPQNLARLPEIVSTAGELVHTNFPSENIEQLLALADKLQDDKPGQYVFQPPLW
                     ASHPPRIETNGRSVQFLKLDAIAALSRQVFGDKSLYEAGGEVPSFAPTTLPSATPGPS
                     PTVENPC"
     gene            4297..5193
                     /locus_tag="VIF08_04190"
     CDS             4297..5193
                     /locus_tag="VIF08_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135217.1"
                     /translation="MALLLTLSLCAPVGAASAPVLPEAPNCPMFPISNVWNKPVTGLP
                     VRSDSDTLVRSIGLNAYLHPDFSSYDGGGYGIPYNVVGGGRKKKYVKFTYASESDRGP
                     YPIPRRPKIEGGSDRHILIVDRDNCVLYELYAARKTSAGWRAGSGAIWNLNSNALRPA
                     GWTSADAAGLPILPGLVRYDEVAAGVIRHALRFTAPVTRRAYIFPARHYASDSNDAAL
                     PPMGLRVRLKATVDISRFGPQSRVLLQALKDYGMILADNGSPWYVTGAPNAGWDDDDM
                     HQLQTLHGSDFEVVDTSSLVNG"
     gene            complement(5298..6011)
                     /locus_tag="VIF08_04195"
     CDS             complement(5298..6011)
                     /locus_tag="VIF08_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135218.1"
                     /translation="MARDDDRRLARASRVLRRRLRLRQVDMRCPRHVVQAIEAGRAGR
                     LTLDVIQRYFEEFDARARLTLWWNGADLDRLLDSRHAGIVEAGLGEVRTYGWKTASEV
                     TFSEFGERGSIDLMAAKEAASAVLIGEAKSEWGSIEETLRRLDVKARLAPKIAFQTFG
                     FRPRAVAAVLLFAEDRTARRVAARFSTTLDSALPARGRDIRRWLREPAGNLRGLWFLT
                     DAADDRHARDKKARRRRNG"
     gene            complement(6035..6946)
                     /locus_tag="VIF08_04200"
     CDS             complement(6035..6946)
                     /locus_tag="VIF08_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEY8135219.1"
                     /translation="MRQPVVSPRVAAALAAGRPVVALESTLITHGFAPPLNLEAARRA
                     EAAVRAGGAEPATVAVRDGMLVVGLSDDELTALAAAPAPRKASRSTVALALVEGGWAG
                     TTVAATMIAAHLAGIRVFATGGIGGVHRGGAATMDVSADLDELAQTPVLVVCAGPKSM
                     LDIDLTLEYLETSGVPVLGWGTTELAGFYARSSGRTLAGSVADAATAARVAAAHWALG
                     LSSGLVIAAPLSAEAALPADEAEAAVAQATADAAAAGVHGPAATPYTLARVAELTYGR
                     SVKANLALIENNASIAARIAAALITAE"
     gene            complement(6943..7833)
                     /locus_tag="VIF08_04205"
     CDS             complement(6943..7833)
                     /locus_tag="VIF08_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8135220.1"
                     /translation="MTIGDLTLDIVVRQSARIESGTDVPAAIQFRAGGSAANTARAFA
                     RLGGAATFVGAVGDDRLGGLLAAALRREGVRVEVARNKGHTARLVVSLAQTGERSFLT
                     DRGTADSLRWGPTLPKLLKATDALHLPAYSLLKAPLAETALRAARVARAAGALVSVDL
                     ASRRPLLAGGSVAARATIAAVKCDVLFANHEEAAALVGPRNLPQLLELAPIVVVKAGA
                     AGCRVLWRDVDMEIAARPFKATDTTGAGDGFDAGFMYSLISTGRALGELRRLDLRHAA
                     YDGGKAAATFIRGPRSELAL"
     gene            7922..9772
                     /locus_tag="VIF08_04210"
     CDS             7922..9772
                     /locus_tag="VIF08_04210"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8135221.1"
                     /translation="MTDSNQDPNYWHELAQQLRVDSVRSSSLAGSGHPTSSLSAAELM
                     AVLIAKHLRYDVSSPRAANNDHLIFSKGHASPLYYAILLAIGAIGEDEFQTYRRLGSR
                     LEGHPTPVLPWVDVATGSLGQGLPIGVGVAMAGKELDRLPYRVWVLCGDSELAEGSIW
                     EAAAQASYDKLDKLVAILDVNRLGQTGPTRYEWDLDIYAERFKAFGWQTIEIDGHDVA
                     QVDAAYTKAAATEGQPTAIIAKTVKGKGVSAVENKGGAHGKPVPDEAAAIEELGGLRN
                     IRLKPQLPEGKATAHAFRGGGALALPTYEKGTSVATRKAYGDALLALGRADPKIVVLD
                     GEVGNSTYTETFAKEIPERFFQMYIAEQQMLAAGVGMHVRGWKPFAATFAAFLTRAYD
                     FARMAAVSRANICLCGSHAGVSIGEDGPSQMGLEDLASMRAIFGSTVVYPCDANQTAR
                     LLALLADQPGVTYMRTTRQATPVIYDADEEFELGGSRVLRSSGDDALAIVTAGVTVPE
                     ALAAAAALANDGIAARVIDLYSVKPLDLATLSDAATATGHVLTVEDHWPEGGIGEAVF
                     GALAAQGVGVKGELLAVRHMPGSATPEQELAEAGIDRDAIVAGAKRLIGP"
     gene            9786..11474
                     /locus_tag="VIF08_04215"
     CDS             9786..11474
                     /locus_tag="VIF08_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135222.1"
                     /translation="MTYAAGHSRRLPADLEKEVAAIQARAADEDWAGRLWSRDASLWT
                     GDEEVAALIANRLGWLDLPTHFSDDVEALAAFARGVRDEGFDAALVCGMGGSSLAPDV
                     LAASFGVTDGGIPVRILDSTDPAAVAQATAASDPARTLYLISSKSGTTTETLAFLAHF
                     WQAEDDIHADIPQGLAGQHFVAVTDPPPSLHAIPHSDLMREVFLNPPDVGGRYSALSY
                     VGLVPGALMGLDLRQMLDAAAQMAEDTRSPSLDNPGVWLGATLGALAKAGRDKLTLVI
                     EPRVARLGAWIEQLVAESTGKHGVGIVPVDGEGLGAPKSYGSDRVFVRISSGEDATWQ
                     ASTTAALDALAAAGHPVVDLATDAGIGGLGAEFFRWEFATAVAGIVLGINPFDEPNVT
                     ESKDNTKRVLEQFGRTGTVTLPAAIDPASLDRHLKRVTPNGYFAIQAYIALSESRDRV
                     LREAADALRDATKQAVTVGYGPRFLHSTGQLHKGGAPIGCFILLTAAHGDDLPIPGSH
                     ESFGTLIDAQAAGDFASLEAHGLPVARVDLGNDPDAGLEAFVTALRQALASPLE"
BASE COUNT         1893 a         3950 c         3986 g         1768 t
ORIGIN      
        1 gctcatcgtg cccggatcca cgttgatata cggatcgagc ttgatgatcg agacagagag
       61 tccgcggctc ttgagcagcc ggccgatgct ggcggcagtg atgcctttgc cgagcgagga
      121 ggtcacgcct ccggtcacaa agacatactt cgccatcgaa tcctggccct cgtgtcgtgg
      181 ctggagcgcg tcgcattcag cgggaacgcg cgagcacgga cgccaacgcc acactcggga
      241 agtgagtgta cctcaatagc cagccgcggc gccagcatct gacgcaaccg agggtcggtc
      301 aggggagcgc tagggggtcg gcacgggggt tcgcagcggg gtccagccgg gggccgggat
      361 gcacaggatg tcgcccgggc ggatgtcgct gtagtcgtgg cggcccgccc aagggaggcc
      421 gttgaagtcg gctatgtcag ttgggctgac gctgaactgc tcaccgatgc tgaccaggaa
      481 gtcgccggac tggacgctgt aggcctcgta gccgtcgcac tcggctgccg ggttcgggat
      541 cgtcagcaca tcgcccggtt ggatcacata gttgggcggc aggagctgtg gattcgcctc
      601 tatcagcgcc gcgacggaga tgccgtacag gttggcgatc ttcgtcaggt tgtcacccgg
      661 ctgaacctcg taggtgccgc ccgggctggg ccggacgctg ggcgccggcg tgggcgtctc
      721 ggacgacacc gggcttggcg agggcgagaa cgtcggcagg ggtccggtgt tcggcggtgg
      781 agtcgtcgtc gcggtcgcgc caccgggcac ggcgagcaga ttcgtgaaca ggccaacacc
      841 tatgaccgtc gtcgcgacgg ccaccacgag caggatgttg acgatcaggg aaaggcgcat
      901 ccgggcgcga ttctagaccg actgtccgtt cggaggattg atcctgcccg ggccccacac
      961 ctccgggcca cagatgaacg gccggggtgg ttgagagtga gcggcgtgcc agctggccat
     1021 gtcggcaagg gagcgcggca gccggccgtc atcgaacatt gcgccgacct gcgcgcgcaa
     1081 gtcggcgaaa tcgcgccact cgtagaacgg ccagccttcg gcggcgcaga tgcgcgtgag
     1141 cgcgccaatg gcgaacgtca cgtctgcatg cgcggccgcg tagcgatcgc tgatgccatc
     1201 accgatgaac acgacgacgc ggccggcggc ctggtggagt cgcacacgct cgcgcttgca
     1261 ggtgccgcac acgaagcacg ccgggtggct gtaggggaac gacatgcccg cgccgccgtc
     1321 ggccacggtg ttgtcgttgg tcgccacagg caggtcggcg acgccgagcg cggcaaggtt
     1381 gggtcgaacg tagaacccga gcccgtcgct gaccacctcg acgagcgcgc cgcgctcgcg
     1441 gacggcctgg acaaacccgg ggaagccccc gtcctggggc atccgcgccg cctcattgcg
     1501 gagcaattcc gcgtcgtgcg gtaggacgtc catatcccat cgcatcagct cgcgcgagcc
     1561 gacctcgccg gcgtcgtact gcgcgtcctt gcgcgcgatc tcggccttat ccgggtagtg
     1621 ccgtgcgagc agctcgtcgc cgatatcgac gcgcgagatc gtgccgtcgt aatcgaccag
     1681 gaagctgacc ggtgcgccgg ttgcgtcggc cggctcggga aggccggccc ggactccgat
     1741 ctgtggcggg ccgctcaggc ccaacgcgga tgccctcgga tcagcttgcg ccgtctgtgg
     1801 agtccggctc caggtccatg gcttcgtcat cggcttcctc ggtcatcttg agccagatga
     1861 agagaccggc gagtgccacc aggggcacga cgatgaccgc cgccagcagc agcacgaaga
     1921 ggagcacgaa caatttggtc aaacgcttca caggaacctc ctgttgactt cctggagcca
     1981 gcgtagcgca tgttgccgcg aaacccggtt gggcgcctcg cccggtccgg cgactcagcg
     2041 gccgcgggcg cgcatcccgg cggcgatcat cgcgccgatg tcctcgcgcg tgaactccac
     2101 gatcatgtgg cgcagcccag cgcgcgtctg catcagcagc cgcacaacga actgctgctc
     2161 ctgctcgaag aagaagatgt cgcgcggctg ctgctggtcg agatacacgc ccagcgcgcg
     2221 caacgcgggc tcgtagaacg cctgggcgtt gggcgctggt ggcccggcgg gcggtctgcg
     2281 cctggtcacg ccctcctcga cgaagcgcgc gatgtcgtcc tcgcgcagca cgacggtttc
     2341 cttgtggagc cgcgcggccg gatcgttcca cggccgctcc tcgtcagcga ccagtgcgag
     2401 gccctggatg atgaagccgt cttcggtctc gttgagcgtt atctcgcgca tcccgcgctg
     2461 gtcaagcagc gcgccgatcg agcgcagcac gtcctcgtag ttctggcgtg gcgctccctc
     2521 gtagatgcgc atctagcagg gattctccac cgtgggcgac ggcccgggag tggccgaagg
     2581 caacgtggtt ggagcgaagg aaggcacctc gccgccggcc tcgtacaggc tcttgtcacc
     2641 aaacacctgc ctggacaggg ccgcgatcgc gtcgagcttg aggaactgca cagatcggcc
     2701 gttggtttcg atccgcggcg ggtggctggc ccagagcggg ggctggaaca cgtattggcc
     2761 aggcttgtcg tcctgcagct tgtcggccag cgccagaagc tgctcgatgt tttcggacgg
     2821 gaaattggtg tgcacgagct cgcccgcggt gctcacgatc tccggcaggc gcgccaggtt
     2881 ctgtggagtg agcaccgcct tcctgagcgc gccgagcagc tcctgctggc gccgggcgcg
     2941 cgcgaagtcg ctgctgccgt ggcgcgaccg ggcataggca agggcggttt cgccgtcgag
     3001 gtggtggggc cccgtctcga gggtgaaacc cacgtggccg tcggtccagt ggtagcccga
     3061 gtcctggatc agcttcgaat tgcagatatc cacaccacca acctcgtcga tcaagtgcgg
     3121 aaagccgcag atgttgatgg aggcgtagta gtcgatcggg atccccaaca ggtagccgag
     3181 ctcgtaggcc agcgccggca ggcccggctc cggaaatacc tcgggatgtt gcttcgtgta
     3241 gcccgtaaaa gtgttcaact tccagtagcc gggcatcgag ccgccgacgt acagcgggaa
     3301 acctgcgctg tcgcgcggga agctgaacat gaagacttcc ccggtgtcgg cgttggcgct
     3361 ggccacgatc atcgtgtcag tgttggcgcc ggtcaccacg cccaccccgg cacagcgact
     3421 gtcaatgccg accaacagca cgttcagcca gccatcttcc agagagagct tggtgatgtc
     3481 gaaggagggc gacgggccgg gagcgatagt cgcgggcggc tcggacggct caggcgtgtc
     3541 ctcgggcggg aaggtcgggc cggacggcgg cggggtggtc ggtaccacgg cgatgccgct
     3601 cggcgttggc cccggcgggc agtcgagggt ggggtcgcag ccggtgccga agttacggtc
     3661 ggctagcgag aggcggtaca tccagtccgc gccgtagagc gtcccggccg caagcgccac
     3721 gatcaggatc gccggcgcta tgaacgcgtt ggtcccggta tgacggccgc cgcgccatgc
     3781 gttgatgacg ttgatgatcc accacactcc gaccagcagc gcgagcaggg cagcgcccag
     3841 ggcgatgctc gggttgagca gctttgcccc gacgcggttg accccgtcga gggacacgaa
     3901 ggccagcagc acggcgagca gcacgacgat gggcgcggca aagatgatcg ccgcgcgccg
     3961 gtcgcgcaga tacgcctgac caaggcccgg gaagatgaag ctcagtgccg ctgccgcgac
     4021 gcctgatcgc tcagacctcc cggacaggcc cagctgcact ggctggaatt cacgattcaa
     4081 gctgagggcg cgcctcctgc tgtcgggcat aactctattc gcagcacgct tgacgcgtca
     4141 tgactgggtg ccgttccctc gtggcaacgg ccgaaggtac gtggcccaat ggggcactgg
     4201 gcaggttgag cagatcgctc gatgtgggcg atgatgacgg agatgaacgc cgtgtgttcg
     4261 tcgagccgag gtcgggcgac gccgctcgct ctcggcttgg cgttattgct tacgctcagc
     4321 ctgtgtgcgc cggtcggcgc ggcgtcggcc cctgttctgc cggaagcgcc gaactgcccg
     4381 atgttcccga tcagcaatgt ctggaacaag cccgtgaccg gcctgcccgt gcgctccgac
     4441 tcggacacac tcgtccggag catcggcctg aacgcctatc tccaccccga cttctcgtcg
     4501 tacgacggcg gcggctacgg cattccctac aacgtcgtcg gcggcgggcg gaagaagaag
     4561 tacgtgaagt tcacctacgc gtcggagtcg gaccgcgggc catatccgat tcccaggcgg
     4621 cccaagatcg agggcggcag cgaccgccac atcctgatcg tggatcgcga caactgcgtg
     4681 ctgtacgagc tctacgcggc gcgcaagacc tcggccggct ggcgggcggg ctccggcgcg
     4741 atctggaatc tgaactcgaa cgcgctgcgg ccagctggct ggacgtcagc cgacgccgcc
     4801 ggcctgccga tcctgcctgg gctggtgcgc tacgacgagg tcgcggcggg cgtcatccgc
     4861 cacgcgctgc gcttcaccgc gccggtcacg cgacgggcgt acatcttccc ggcgcgccac
     4921 tatgcgtccg attcgaacga cgcggcgctg cccccgatgg gcctgcgcgt ccgactgaag
     4981 gccaccgtcg acatctcacg tttcggcccg cagtcgcgcg tgctcctcca ggccctcaag
     5041 gactacggca tgatcctcgc cgacaacggc tccccctggt acgtcaccgg cgcaccgaac
     5101 gcgggctggg acgacgacga tatgcaccag ctgcagacgc tccacggctc cgatttcgaa
     5161 gtggtcgaca cctcctcgct ggtgaacggc tagcggcgcc cctgccgcgc ccgttcctgc
     5221 caaatgctgg ccaggacggc cgctgccggg ccttggactc ggtatcctga cacctgccta
     5281 tccaggacga gcggctgtca accgttgcgg cgccttcgag ccttcttgtc ccgcgcgtgc
     5341 ctgtcgtcag cggcatctgt caggaaccac aggccgcgca gattgcccgc cggctctcgc
     5401 aaccaacgcc gaatgtcgcg tcccctggcg ggtagcgcgg agtcaagagt cgtcgagaag
     5461 cgcgcggcaa ctcgacgcgc cgttcgatcc tcagcgaaga gcaggacggc cgcgactgcc
     5521 cgtggcctga atccgaaggt ttgaaacgca atcttgggtg ccagccgggc cttcacatcc
     5581 agccggcgaa gggtctcctc gatcgaaccc cattcgctct tggcctcacc gatgaggacc
     5641 gcagacgcgg cctccttggc ggccatcagg tcgatcgatc ctcgttcacc gaactcggag
     5701 aacgtgactt cacttgccgt cttccacccg taagttcgga cctcgcccaa accggcctcg
     5761 acgatgcccg cgtggcgact atcaagcagt cgatcgaggt ccgccccgtt ccaccaaagg
     5821 gtcagccgag cgcgtgcgtc aaattcctcg aagtagcgct ggatcacatc aagggtcaga
     5881 cgccccgcgc ggcccgcctc gatcgcttga acgacgtggc gtgggcaacg catatccacc
     5941 tggcgcagcc tcaggcgccg tcggagcacc cgggaagcgc gcgcaaggcg ccggtcgtcg
     6001 tcgcgagcca ccaggagatc gtgacgggcg gggcttactc cgcggttatc agggcggccg
     6061 cgatccgggc ggcgatggac gcgttgttct cgatcagcgc aaggttggct ttgacggagc
     6121 ggccataggt gagctcggcg acgcgggcga gggtgtacgg cgtggcagca ggaccgtgga
     6181 cgcccgcggc cgcggcatca gcagtggcct gggccaccgc ggcctccgct tcatcggctg
     6241 gcagcgccgc ctctgccgac aggggcgcgg cgatcacgag gccgctgctc aaaccaagcg
     6301 cccagtgggc cgccgcgacg cgggctgcgg tggcggcatc cgcgacggag ccagcgaggg
     6361 tgcgcccact cgatcgggcg tagaagccgg ccagctcggt cgtgccccag cccaggacgg
     6421 gcacgccgct cgtctccaga tactcgagtg tcaggtcgat gtcgagcatc gacttgggac
     6481 cggcgcaaac gacgagcacg ggcgtctggg cgagctcgtc gaggtcggcg gagacatcca
     6541 tcgtcgccgc gccgccgcga tgcacgccgc cgatgccacc ggtggcaaag acacgaatcc
     6601 cagccaggtg cgcggcgatc atcgtcgccg ccacggttgt gcccgcccag ccgccctcga
     6661 ctagcgcaag ggccacggtc gagcgcgaag ccttgcgcgg cgcgggcgcg gcggcgagcg
     6721 cggtcagctc gtcgtccgaa aggcccacga ccagcattcc gtcgcgaacg gcgaccgtgg
     6781 ccggctcggc gccgccggcg cgcaccgcgg cctcagcgcg gcgggccgcc tcgaggttca
     6841 gaggtggcgc gaagccgtgg gtgatgagcg tcgactccag cgccacgact ggccggcccg
     6901 cagcgagcgc cgcggcgacg cgcggcgaga ccaccggctg cctcacagcg cgagctcgct
     6961 gcgtggcccg cggatgaatg tggccgcggc cttgccgccg tcgtacgcgg cgtggcgtag
     7021 gtcgagccgg cgcagctcgc ccagggcgcg tcccgtcgag atgagcgagt acataaagcc
     7081 tgcgtcgaag ccgtccccgg cgccggtcgt gtcggtcgcc ttgaagggtc gcgccgcgat
     7141 ttccatgtcc acatcgcgcc acagcacgcg acagcccgcc gcgccggcct tgacaacgac
     7201 aatcggtgcc agctcgagca actggggcag gttgcgcgga ccgacaagag cggccgcctc
     7261 ttcatgattc gcgaaaagca cgtcgcactt cacagcggcg attgtcgccc tggccgcgac
     7321 cgacccgccc gccaacagcg gtcgccgcga cgccagatcg acggacacca acgcacccgc
     7381 ggcccgtgcc accctcgcgg cgcgcaacgc tgtctcggcc agcggcgcct tcaaaagcga
     7441 gtaggccggc aggtgcaacg cgtctgtggc cttaagcaac ttcgggagtg tcggccccca
     7501 gcgcagtgaa tcggccgtcc cccggtcggt caggaatgag cgctcgccgg tctgggcgag
     7561 ggagaccacg agtcgcgcgg tgtggccctt gttgcgcgcc acctcgacgc gaacgccttc
     7621 gcgcctcaat gccgcggcca gcaggccgcc cagacggtca tctccgaccg cgccaacgaa
     7681 cgtcgccgcg ccacccagac gagcgaacgc gcgcgccgta ttcgccgccg aaccaccggc
     7741 tcggaactgg atggcggcgg gcacgtccgt gccgctctcg atccgggccg actggcgcac
     7801 gacgatgtcg agcgtcaaat cgccaatggt caccagacgc ggtcttccgg gcggcactcc
     7861 ggccatgata tgaagccttg acgccgcacg aatgacttcc cgctaggcta ggcgacagcc
     7921 catgaccgac tcgaaccagg atcccaacta ctggcacgag ctcgcgcagc agctgcgggt
     7981 cgactcggtg cgctccagca gcctcgccgg ctccggccat cccacctcgt cgctctccgc
     8041 ggctgagctc atggccgtgc tcatcgccaa gcacctgcgc tacgacgttt cgtcgccgcg
     8101 cgcggcgaac aacgaccacc tgatcttctc caagggccat gcgtcgccgc tgtactacgc
     8161 gatcctgctg gccatcggcg cgatcggcga agacgagttc caaacgtacc gccgcctggg
     8221 cagccggctc gagggccacc caacgcctgt gctgccgtgg gtggacgtgg cgaccgggtc
     8281 gctgggccag gggttgccca tcggggtggg cgtcgcgatg gcaggcaaag agctcgaccg
     8341 cctgccctat cgggtgtggg tgctgtgcgg cgacagtgag ctcgccgaag gctccatctg
     8401 ggaggccgcc gcccaggcgt cgtacgacaa gctcgacaag ctcgttgcga tcctcgacgt
     8461 caaccgcctg ggccagaccg gacccacaag gtatgagtgg gacctcgaca tctatgccga
     8521 gcgtttcaag gctttcggct ggcagacgat cgagatcgac ggccacgacg tcgcacaggt
     8581 ggacgcggcg tacacgaagg ccgccgcaac ggaagggcag ccgacggcga tcatcgccaa
     8641 gaccgtcaag ggcaaaggcg tcagcgccgt cgagaacaaa ggcggggcgc acggcaagcc
     8701 cgtgcccgac gaggcggcgg cgatcgagga gctgggcggg ctgcgcaaca tccgcctcaa
     8761 gccgcagctc cctgaaggca aagcgacggc gcacgcgttc cgcggcggcg gcgccctggc
     8821 cctccccacc tacgagaagg gcacgtccgt tgcgacgcgc aaggcctacg gcgatgcgtt
     8881 gctggccctt ggccgtgcgg accccaagat cgtcgtgctc gacggcgagg tgggcaactc
     8941 gacctacaca gagacgttcg ccaaggagat ccccgagcgc ttcttccaga tgtacatagc
     9001 cgagcagcag atgctcgccg cgggcgtcgg catgcacgtc cgcggctgga agccgttcgc
     9061 cgccaccttt gcggcgttcc tgacgcgcgc ctacgacttc gcgcgcatgg ccgcggtctc
     9121 gcgcgcgaac atctgcctgt gtggctcgca tgccggtgta tcaatcggcg aggacgggcc
     9181 gtcgcagatg ggccttgagg acctggcctc gatgcgcgcg atcttcggct cgacggtcgt
     9241 ctacccgtgt gatgcgaacc agaccgcccg attgctcgcg ctgctggccg atcaacccgg
     9301 cgtgacctac atgcgcacca cccgccaggc gacgccggtc atctacgacg ccgacgagga
     9361 attcgagctt ggcggcagcc gcgtgctgcg ctcgtctggt gacgatgcgc tggcgatcgt
     9421 cacggcaggg gtgacggtgc ccgaggcgct ggcagcggcc gcggcattgg ctaacgacgg
     9481 catcgctgcg cgggtgatcg acctgtactc cgtcaagccg ctcgacctgg ccacactcag
     9541 cgacgcggcg acggcaaccg gtcacgtgct gaccgtcgaa gatcactggc cggagggcgg
     9601 catcggcgag gcggtgttcg gcgcgctcgc cgcacagggc gtcggcgtca agggtgagct
     9661 gctggcagtg cgccacatgc ccggctcggc cacgccggag caggagctcg ccgaagcggg
     9721 catcgaccgc gacgcgatcg tcgccggcgc gaaacgactg atcggccctt gatggcggcg
     9781 cgctcgtgac gtacgcagcc gggcacagtc ggcgcctgcc cgcggacctc gagaaggaag
     9841 tggccgcaat acaggcacgc gccgccgacg aggattgggc gggccgcctc tggtcgcgcg
     9901 acgcgtcgct ctggactgga gacgaggaag tcgccgcgct gatcgcgaac cgcctgggct
     9961 ggctcgatct gccaactcat ttcagcgacg acgtcgaggc gcttgccgcg ttcgcgcgcg
    10021 gcgtgcgcga cgagggcttc gacgcggccc tggtgtgcgg catgggtggc tcgtcgcttg
    10081 ctccggacgt gctcgccgcc tccttcgggg tcacggacgg cgggatcccg gtacgcatcc
    10141 tcgattcgac cgatccggcc gcggttgccc aggccactgc tgccagcgat ccggcgcgga
    10201 cgctttatct gatcagcagc aagagcggta cgacgacgga gacgctcgcc ttcctggccc
    10261 acttctggca ggccgaggac gacatccacg ccgacatccc gcagggtctc gccggccagc
    10321 acttcgtggc cgtgactgat ccgccgccca gcctgcacgc gatcccgcac agcgacctga
    10381 tgcgcgaggt gttcctgaat ccgccagatg tcggcgggcg ctacagcgcg ctgtcgtacg
    10441 tcggcctcgt gccgggggcg ctgatgggcc tcgacctgcg ccagatgctc gacgccgcgg
    10501 cccagatggc ggaagacacg cgcagcccca gcctcgacaa cccgggtgtc tggctgggcg
    10561 cgacgctcgg tgcgctggcc aaggccggtc gcgacaagct gacactcgtc atcgagccgc
    10621 gcgtggctcg actgggggca tggatcgagc agctcgtcgc ggagagcacc ggcaagcacg
    10681 gcgttggcat cgtgcccgtt gacggcgagg gacttggcgc cccgaagagt tacgggagcg
    10741 atcgcgtctt cgtgcgcatc tcgtctggtg aagacgccac gtggcaggca tcgaccacgg
    10801 ccgcactgga cgcgctcgcc gcggctggcc atcccgtcgt tgacctcgcc accgacgcag
    10861 gcatcggcgg ccttggcgcc gagttcttcc gctgggagtt cgcgaccgcc gtggccggga
    10921 tagtcctggg catcaacccg ttcgacgagc cgaacgtcac cgaatcgaag gacaacacca
    10981 agcgcgtcct cgagcagttc ggccggaccg gcaccgtcac ccttccggca gcgatcgatc
    11041 cggccagcct cgatcgccac ttgaagcggg tcacgcccaa cggctacttc gccatccagg
    11101 cgtacatcgc gctgagcgag tcacgcgatc gcgtcctgcg tgaagcggcg gacgccctca
    11161 gggatgcgac gaaacaagcc gtgaccgtcg gttacgggcc gcgcttcctg cattcgaccg
    11221 ggcaactgca caagggcggc gcgccgatcg gctgcttcat cctgctgacc gcggcgcacg
    11281 gtgatgacct gcccattccc gggtcgcacg aatccttcgg cacactcatc gacgcccagg
    11341 ccgcgggcga cttcgcctcg ctggaggcgc acggcctgcc cgtcgcgcga gttgacctgg
    11401 gtaacgaccc cgacgccggc ctggaggcgt tcgtcacggc gctcaggcaa gccctcgcgt
    11461 caccactgga gtaaggagag gatcgtggag ctggccattg ccggactcgg ccgcatgggc
    11521 ggcaacatgg cccgtcgact gcacaaggcg ggcattcggg tggtcgctta caaccgctcg
    11581 cgcgacaaga ccgagga
//
LOCUS       DATBRX010000119         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3501486, whole genome shotgun sequence.
ACCESSION   DATBRX010000119 DATBRX010000000
VERSION     DATBRX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3501486"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VIF08_04220"
     CDS             complement(<1..500)
                     /locus_tag="VIF08_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135223.1"
                     /translation="MSRLTRFVALSVIGVALVAGPAATTASAGVLDYGAIARCKYAVT
                     QSHNGTWTEALLKRIVVRPPTLPAMTTMDSVGWRFVILRSLDRENTPYVITYRSPLQR
                     APRSADFTPMRHKLHVPAEADTPNGRSFVSYQVMLKFYWYKADGSLFTSAVYTMQNMD
                     LVIAGE"
     gene            897..2162
                     /locus_tag="VIF08_04225"
     CDS             897..2162
                     /locus_tag="VIF08_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEY8135224.1"
                     /translation="METWIAAAVFVAAYALIALERFDRTLIALLGGLLVVALGVIDQH
                     EAFAAIDLNVIFLLAGMMVIASIVARTGLFDWLALRSVRLVHGRPLPLLLVLCTVTAV
                     LSAFLDNVTTVVLIIPVTLSVARRLGVSPVPYLISQILASNIGGTATLIGDPPNILIG
                     SAAGLDFNAFLLNLGPVSVIVFVVFLLMMPLFFRRGLQVPDEAREAALEASAESAITD
                     RPLLVKSLAVVALTMLGFLAHQALGLEPATIALLGATVLMLIASIDPHDVLREIEWKT
                     LFFFVGLFVLVEAVVAVGIVGGIADMLATAVGGDATVASIGLLWFSALASAIIDNIPY
                     TATAIPIVERLIESGLPADPLWWSLALGACLGGNLTIVGASANVVVVNTAAREGHRIG
                     FLEFMRYGALVTLMSLAISTVYVWLVYLA"
     gene            2159..2326
                     /locus_tag="VIF08_04230"
     CDS             2159..2326
                     /locus_tag="VIF08_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135225.1"
                     /translation="MRGSWWRDPDYRRDALILVGLFGLGMLINILVGVAMLVLGDLLG
                     PWSAEYLARDT"
     gene            2443..2928
                     /locus_tag="VIF08_04235"
     CDS             2443..2928
                     /locus_tag="VIF08_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8135226.1"
                     /translation="MTDELRAQLVQLLRGTQAHMSFDEAVADFPAWAINQRAPDVTYT
                     PWHLIEHLRITQWDMLRYIEDPTEHVSPDWPVGYWPDPAAETDTTGFARSVASFKADL
                     RAMEQIALDESRDLSAVLDGTPGHTPLRGLMIIGNHNSYHLGEFASLRQVMGSWPDGH
                     S"
     gene            complement(2933..>3761)
                     /locus_tag="VIF08_04240"
     CDS             complement(2933..>3761)
                     /locus_tag="VIF08_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135227.1"
                     /translation="CAVRADGTVWCWGEDPGQDPSTLLPRRVAGINDAQFVTAGGAFA
                     CALRETHQVVCWGNNALGQLGTGSFSAGEATAQPVEGLSDAVEVNAGWNHACAIRFDR
                     TLWCWGGNGDGATGYGQLGDGTLQNRASPVQVSLPADVISVAAGGWTTCATLVDDTVW
                     CWGYGERGTLGDGNFSNSSVPVQVVGLDNVGLLALGDYTACASRDDDSVWCWGDTSWG
                     SSTGGPAGTPVEGNKSSSIAVGALANDRYCLFIDGREQVWWWGTGTNQAPERWPVGP"
BASE COUNT          547 a         1277 c         1260 g          677 t
ORIGIN      
        1 tcttcgccgg caatgacgag gtccatgttc tgcatcgtgt atacggcgct cgtgaacagc
       61 gacccatctg ccttgtacca atagaacttg agcatcactt ggtaggagac aaagctgcgg
      121 ccgttggggg tgtcggcctc ggccggcacg tgaagcttgt ggcgcatggg cgtgaagtcg
      181 gccgaccttg gtgcgcgctg gagggggctc ctgtacgtga tcacgtatgg ggtgttctcg
      241 cggtcgagtg accgcaggat cacgaaacgc cagccaacgc tgtccatggt ggtcatcgcg
      301 ggcaacgtcg gcgggcgcac aacgatcctc ttgagcaggg cctcggtcca ggtaccgttg
      361 tggctctgag tcacggcgta cttgcacctg gcaatggcgc cgtaatcgag gactcccgcc
      421 gatgcggtgg ttgcggccgg tcctgcgacc agggccacgc caatgaccga aagagcgacg
      481 aaacgggtca atctcgacat gtgactacct ccttgatcag ttgatgcccc tcgagcggca
      541 ggtgacaccc gtctgagtac ggaaagcgct acggataggc gccgtgcgta cagcgcatcc
      601 aactcccgcc cgcggaggca cagtcgccgg tggagcagct cgcctggatc cagtcggcgg
      661 acgcggagca gcgccgcgcc gcctgaacgc ggcgaggtcc ggtttgacgc gaccggttac
      721 cctgtcgcgg acatgactcg gctcgagacg acgaacgcgg cccatgagct cgcgtcgcag
      781 gtggtggcgg cctgcgacgg ggcggtttgg tagcgcggcg aacatctgat cgtcttcctc
      841 tagatcaatc gccgggcggc gcgactcgcc gtcgcggcgc gcggccgcgt taggccgtgg
      901 aaacctggat cgcggccgcg gtcttcgtcg cggcctacgc gctgattgcc ctggagcgtt
      961 tcgacaggac gctgatcgca ctcctgggcg gcctgctggt cgtcgcgctg ggcgtgatcg
     1021 atcagcacga ggctttcgcg gcgatcgacc tcaacgtcat cttcctgctc gcagggatga
     1081 tggtcatcgc ctccatcgtg gcccgcaccg ggctgttcga ctggctcgcc ctgcgctctg
     1141 tccggctggt gcatggccgg ccactgccat tgctgctcgt gctgtgcacg gtcacagccg
     1201 tactgtcggc cttcctcgac aacgtcacca cggtcgtgct gatcattccc gtcaccctgt
     1261 ccgtcgcgcg ccggctgggc gtctcgcccg tgccgtacct gatctcgcag atcctggcgt
     1321 cgaacatcgg tggcacggcc acgctgatcg gtgacccgcc gaacatcctg atcgggtccg
     1381 ccgcggggct cgacttcaac gcgttcctgc tgaacctggg tccggtgtcg gtgatcgtat
     1441 tcgtcgtctt cctgctgatg atgccgctgt tcttcaggcg cggcctgcag gtgccggacg
     1501 aggcgcgcga ggcagctctc gaggccagcg cggagtccgc catcacggat cgcccgctgc
     1561 tggtgaagtc gctcgccgtc gtggcgctca cgatgctcgg cttcctcgcc caccaggcgc
     1621 tggggctgga gccggcgacg atcgcattgc tcggcgcgac ggtgctgatg ctgatcgcct
     1681 cgatcgaccc acacgatgtg ctgcgcgaga tcgaatggaa gacgctgttt ttcttcgtgg
     1741 gcctgtttgt cctcgtggag gcagtcgtcg ccgtgggcat cgtgggcggc attgccgaca
     1801 tgctcgcgac ggccgtgggt ggtgacgcga ccgtggccag catcgggctg ctttggttct
     1861 cggcgctcgc gtcggcgatc atcgataaca tcccttacac ggcgaccgcc attcccatcg
     1921 tggagcggct gatcgagagc ggcttgcccg ccgatccgct gtggtggtcg ctcgcgctgg
     1981 gggcgtgcct gggcggcaac ctcacgatcg tcggcgcgtc ggccaatgtc gtcgtggtca
     2041 acaccgccgc gcgcgaaggg catcgcatcg gcttcctgga gttcatgcgc tacggcgccc
     2101 tggtaaccct gatgtcgctc gcgatctcga ccgtgtacgt ctggctggtg tatctcgcgt
     2161 gaggggttcg tggtggcgcg atcccgacta ccggcgcgat gcgttgatcc tggtcgggct
     2221 gttcggtttg gggatgctga tcaacatcct ggtcggcgtg gcgatgctcg tgctgggcga
     2281 tctcctcggg ccctggagcg cggagtacct ggcgcgcgac acgtagcgcc tggtccgtag
     2341 cgcctcgggt ggccgcacgg tgccacttgg agcgtgagcg gcatctcctg cttttgtccg
     2401 gctgcaatac gtcggctgac tcgcggagcc acaatcgggg gcatgaccga cgaactccga
     2461 gcccagctcg ttcagctgct gcgcggcacg caggcacaca tgtccttcga cgaagcggta
     2521 gccgacttcc cggcgtgggc gatcaaccag cgcgcaccgg acgtgacgta cacgccctgg
     2581 cacctgatcg agcacctgcg catcacgcag tgggacatgc tgcgctacat cgaggacccg
     2641 accgagcacg tctcgcccga ttggccggtc ggctactggc ccgacccggc agccgagacc
     2701 gacaccactg ggttcgccag gtcggtcgcg agcttcaagg ccgacctgcg cgccatggag
     2761 cagatcgccc tcgacgagag ccgcgatctc agcgcggtgc tcgacgggac gcccggccac
     2821 accccgctgc gcggcctgat gatcatcggc aaccacaact cgtatcacct gggcgagttc
     2881 gcgagcctgc gccaggtgat gggctcgtgg ccggacggcc acagctagag gcctacgggc
     2941 cgaccggcca gcgctcgggt gcctggttcg tgccggtgcc ccaccaccac acctgctccc
     3001 tgccgtcgat gaacaggcag tagcggtcgt tcgccagggc gccgacggcg atgctcgagg
     3061 acttgttgcc ctccacgggt gtgcccgccg ggccgccggt ggacgatccc cacgacgtgt
     3121 cgccccagca ccacacgcta tcgtcgtcgc ggcttgcgca cgcggtgtag tcgcccagcg
     3181 cgagaagacc cacattgtcg aggccgacta cctgaactgg caccgagctg ttgctgaagt
     3241 tgccgtcgcc caacgtgccg cgctcgccgt agccccagca ccacacggtg tcatcaacga
     3301 gcgttgcgca cgtcgtccaa ccgccggcag ccacgctgat cacgtcggcc ggcaaggaga
     3361 cctgcaccgg gctcgcccgg ttctgcagcg tgccgtcgcc gagctgcccg tagccggtcg
     3421 cgccgtcgcc gttgccgccc cagcaccaga gcgttcgatc gaagcggatg gcgcaggcgt
     3481 ggttccagcc ggcgttcacc tcgaccgcgt cgctcagtcc ttcaaccggc tgcgcggtcg
     3541 cctcgcctgc gctgaagctg cccgtgccga gctggccgag cgcgttgttg ccccagcaga
     3601 ccacctgatg tgtctcccgc agcgcgcagg caaacgcgcc gccggcagtg acgaactggg
     3661 cgtcgttgat gccggccacg cgccgcggca gcagggtgct cggatcctgg ccgggatcct
     3721 cgccccagca ccagaccgtg ccgtcggccc gcaccgcgca c
//
LOCUS       DATBRX010000120         3341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2830529, whole genome shotgun sequence.
ACCESSION   DATBRX010000120 DATBRX010000000
VERSION     DATBRX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2830529"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1354)
                     /locus_tag="VIF08_04245"
     CDS             complement(161..1354)
                     /locus_tag="VIF08_04245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEY8135228.1"
                     /translation="MPRVLHMADVHLGARHDDLGPTAAAQRERQFAAFARAIDLALTE
                     KVDLVLICGDLFDSNSQPRRSVERAAAELGKLVARHVPVVVIPGTHDCYEPESIYRVF
                     DLTEMAGATPDSNLVTVLTDSHPQADFGQLGISVLGHVFPAKRAAESPLVGIKAAADA
                     ARADGPRARNWLIGMVHGSIAQPGRFEHDEVIVTEQEIAQSGLDYLALGHVHSFREGR
                     AGTVTYAYSGAPEPVALDQDGAGQVLLVSLDERDGQRVVLVEPRTVGKTRFRKMEVDG
                     ATVVSQAALGAQLKGLSDPDLVLDARIVGVRSGELDLSTDELTKQLETSFLKLRLRDA
                     SVVALPDDAAAPPDTILGAMTQDFRARIEDHETRGDMERAAELREALRLGTALLDDPT
                     RVSLA"
     gene            1689..2039
                     /locus_tag="VIF08_04250"
     CDS             1689..2039
                     /locus_tag="VIF08_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEY8135229.1"
                     /translation="MSYADRTLTCADCGVEFIHSAADQEFYTQKGFASDPKRCPSCRA
                     SRRQMRETSSGFDVREIGGPRGYERGDYRPQREYFAVICSKCGNAAQVPFKPRQDKPV
                     YCSDCFRDVQGAAG"
     gene            complement(2078..3031)
                     /locus_tag="VIF08_04255"
     CDS             complement(2078..3031)
                     /locus_tag="VIF08_04255"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY8135230.1"
                     /translation="MTERRPNFFQVGAMKAGTTSMHNYLGQHPDIYMSPRKEPRWSSL
                     VPDLDSGGEADARYFTRDRDEYLGHFSGAGDEKVVGESSHVYLPSTQAARLIHEFAPD
                     ARILIMLRDPVTSIHSRHQQNVWMGREDILDFASALAAESDRRAGRRLPPDTPYHAGL
                     LYRAACTMTPQARRFLDEFGRDRVHFALLEDLGAKPLDTYRRVLEFLGVDPDFVPAEM
                     AVVNPSSQLRSVRFHRLRARLWPVERTVRRVVPAGAFRVLSAPLRAAWNANKRPAPRS
                     PMSPQLEAELTEYFAPDVASLSELIGRDLSEAWPRFRRAAG"
BASE COUNT          534 a         1138 c         1134 g          535 t
ORIGIN      
        1 aacgttgggc ggcggaagag ggccatctcg atcgctcgct ggatggtcga cttgccggcc
       61 tcgttggcgc cgcgcaccac gttcaggccg gggccgaact gcacatccag ggcgcggtgg
      121 cggcggaagt tcgtgaggcg cagacgggtg atgatcatcg tcaggccagc gacactcggg
      181 tggggtcatc gagcagcgcc gttcccaggc ggagtgcctc gcgcagctcg gccgcgcgtt
      241 ccatatcgcc gcgcgtctcg tggtcttcga tgcgtgcccg gaagtcttgc gtcattgccc
      301 ccaggatcgt gtccggtggc gccgccgcgt catcaggcag ggcgacgacc gacgcgtcgc
      361 gcaggcgcaa cttcaggaac gaggtctcca gctgcttcgt gagctcgtcc gtgctgaggt
      421 cgagctcgcc agagcggacg ccgacgatgc gcgcgtccag caccaggtcc ggatcggaca
      481 ggcccttaag ctgcgcgccc agggctgcct gggaaacgac cgtcgcgccg tcgacctcca
      541 tcttgcggaa cctggttttg cccacggtcc tcggctccac caaaacaacg cgttgaccgt
      601 cgcgctcgtc gagactgacg agcaatacct ggcccgcgcc gtcctggtcg agcgcgaccg
      661 gctcaggcgc gcccgagtag gcgtatgtga cggtaccggc gcggccctcg cggaacgaat
      721 ggacgtgccc gagggcgagg tagtcgagcc cgctctgcgc tatctcctgt tcggtgacga
      781 tgacctcgtc gtgctcgaac cggccgggtt gagcgatcga gccgtggacc atgccgatca
      841 gccagttacg ggcacgcggc ccatcggcgc gcgccgcgtc agctgccgcc ttgatcccga
      901 cgagcggact ctccgcggcg cgcttggccg ggaagacatg gcccagcacc gatataccca
      961 gctgcccaaa gtcggcctga gggtggctgt cggtcagaac ggtcacgagg ttcgaatcgg
     1021 gcgtggcgcc ggccatctcc gtgagatcga agacgcggta gatggattcc ggctcgtagc
     1081 agtcatgcgt gcccgggatg acgacgaccg gcacgtggcg cgcgaccagc ttgcccagct
     1141 ccgccgcggc gcgctcgacc gagcggcgcg gctggctgtt cgagtcgaag agatcgccgc
     1201 agatgagcac caggtcgacc ttctcggtca gcgccaggtc gattgcccgc gcgaaggccg
     1261 cgaactgccg ctcgcgttgg gcagccgcag taggcccgag gtcgtcgtgg cgagctccca
     1321 ggtgcacgtc ggccatgtgc aggacgcgtg gcatcgcggc tgtttcttgt tacctctgcc
     1381 tcgaacggtg ctggcccgca ccgcaattcg tgctttcaat tctgacagcc cttcggggag
     1441 ggacgacatg ggaaggaagt gctaccaagc gctcggccgg ctcggcccgg agacccgatt
     1501 tcgggtgagt ggtaaccttg ggttcgttac cggtgcgccc agttcatgga cctcggcaca
     1561 gcacctgggt ttgcgcggcg cccgacctct tcgatccgac gccaagcgat ccttacgtgc
     1621 ttgcccggac ccgctgatcg cctcggaaat ggagcttcat ttcagtgctg accacgggga
     1681 gacagctggt gtcctacgca gatcgaactc ttacctgcgc cgattgcggc gtcgaattca
     1741 tccactccgc cgccgaccag gagttctaca cccagaaggg cttcgcgtcc gaccccaagc
     1801 gctgtccctc atgccgcgcg tcgcgacgcc agatgcgcga gacgagcagc gggttcgatg
     1861 tccgcgagat cggcggcccg cgcggctacg agcgcggcga ttaccgaccg cagcgcgaat
     1921 acttcgcggt gatctgctcc aagtgcggca acgcagccca ggtgccgttc aagccgcgcc
     1981 aggacaagcc ggtctactgc tcggactgct tccgcgacgt ccagggcgcg gccggttaat
     2041 cgcggccggc taatcgcggg cggctaagcc cccgccctca tccggccgcg cgacggaagc
     2101 gcggccaggc ctcgctcaga tcgcgcccga tcagctcgct cagcgacgcc acgtcaggcg
     2161 cgaagtattc cgtgagctca gcctcgagct gcggtgacat ggggctgcgc ggcgccgggc
     2221 gcttgttagc attccaggcg gcgcgcaacg gggcgctcag caccctgaac gcgcctgccg
     2281 ggacgacccg gcgcactgtc cgctccactg gccagaggcg cgcgcgcaac cgatggaacc
     2341 tgaccgagcg cagctggctg ctggggttca ccaccgccat ctccgcgggg acgaagtcag
     2401 ggtctacgcc caggaactcg agcacccggc ggtaggtgtc gagcggcttc gcgcccaggt
     2461 cctcgagcag cgcgaagtgg acacggtcac gaccgaactc atcgaggaag cgccgcgcct
     2521 ggggcgtcat cgtgcacgcg gcgcggtaca gcaggccggc gtgatacggc gtgtccggcg
     2581 gcagccgacg tccggcacgg cggtcactct ccgcggccag tgccgaggcg aaatcgagga
     2641 tgtcctcgcg gcccatccag acgttctgct ggtggcgcga gtggatcgac gtcacggggt
     2701 cacgcagcat gatcagtatc cgcgcgtcgg gcgcaaactc atggatcagc cgcgcggcct
     2761 gcgtcgaggg caggtagacg tgtgacgact cgccgaccac cttctcgtcg cccgccccac
     2821 tgaagtggcc caggtattca tcacggtcgc gggtgaagta gcgcgcgtcc gcctcgccgc
     2881 ccgaatcgag atccggcacg aggcttgacc agcgcggctc cttacgaggc gacatgtaga
     2941 tgtccgggtg ctggcccagg tagttgtgca tcgaggtcgt gccggccttc atcgccccga
     3001 cctggaagaa gttgggtctg cgctcagtca cccgcggagg ctacccaaga gcagcggctt
     3061 ccaccaaatg cagaaacggt ctgcacggtc tccggagctt cccgtcagcc gcccaaccag
     3121 tcctgcgcct gatgccgaat gagcgcccaa tgggagcacg cggttgattc cgcgcatgcc
     3181 agtcctgcgt ttgacgggag gagccagcga accgggcgct ccgcccgcgc ggcagcgcgc
     3241 gccagctttg ccgcgcggct ccgctagcgc tgatcgagag gcaggtactt ccgctcgcgc
     3301 gcgccggtgt acacctggcg cggccggcca atccgcgaat c
//
LOCUS       DATBRX010000121         4858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2048841, whole genome shotgun sequence.
ACCESSION   DATBRX010000121 DATBRX010000000
VERSION     DATBRX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048841"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VIF08_04260"
     CDS             complement(<1..632)
                     /locus_tag="VIF08_04260"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135231.1"
                     /translation="MATADQPSVLIVGGFATVPPNYWPLRRRLLARGAARVDIAPLWT
                     PDWVLGALLGFGSVMLRTGRAIGRTWRDGGRKPVIVVGHSAGGIAARLAMADKPFNGR
                     IADVSEAVGCLVTLGTPHGLYRLTNRYRHAGHAATEFLDRATPGAYFAPRTSYLSVGG
                     RTPGAEFPGLAGDLANELFGVAVGKETQAPGDGIVPFSAVHLDGATQLTY"
     gene            complement(637..2622)
                     /locus_tag="VIF08_04265"
     CDS             complement(637..2622)
                     /locus_tag="VIF08_04265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY8135232.1"
                     /translation="MADREVLPYGSWPSPITVDMAVSSSLTLREPRFDGGDLYWTEGR
                     PQERGRQVIVRWNERDGAVDVTPPEFNARTMAHEYGGGWYAVADGTVYFANLADGRIY
                     RQPRGEAPVALTADGPYRYGDLTFDRARARLLCVREDMTRALAAGADAHTAGAGGRIP
                     EPEDALVAIDPASGATTVLAQGYDFYNTPRPSPDGTRLAWLSWRHPNMPWDSTELWVA
                     HLDAAGLPVDPQLIAGGPDESVIQPEWAPDGALVFISDRSGWWNLYRWTGAGRADEIR
                     PIAPMDAEFGGPLWVFGLSWYGLADDGTIVAMVRRAGTDELWRFPVRGAAARIDLPFA
                     TVGLVQVNGRRVAFEAAGSTEPLSVVLLDLDSGEPLILRRSFELAVDPAYVSMPEEIT
                     FPTSDGDVAHAWYYPPTNPGVAGPPDARPPLVVHNHGGPTSLAPAMLDLDKQFFTSRG
                     FAVVDVNYRGSNGYGREYMRKLYGTWGIYDVDDCINAARYLAQRTDVDPARMAIRGGS
                     AGGYTTLCALAFHDVFAAGASYFGVGDIEALARETHKFESRYMDQLVGPYPEAMALYH
                     ERSPIYHVEGFSKPLLVLQGRDDMVVPEAQSEQIVAALKERRIPHAYLAFEGEGHGFR
                     QAANIRRSLEAEASFYAQVFGFILADDFAPLKIEFLS"
     gene            2691..3338
                     /locus_tag="VIF08_04270"
     CDS             2691..3338
                     /locus_tag="VIF08_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135233.1"
                     /translation="MAERDGAPPPEQRHTGVVRRSDLTRPRPSTVRQDWLALVGVAAL
                     MVIVLVVIANFFGGGPPADPSSSPTMVANASGTPTLKPTYSAEPTRTPTPTPTPSPTP
                     SPTATPSPTPTPTPTPASTPAPTPAPTAPSAGLIILEPAEGSTVDRVVNVSGLTQPGA
                     TITRVKQFWFDDHVIADEAGRWTLAVELSPGWNAIEFRVADDPSTQVIVNLYYAG"
     gene            3338..4216
                     /locus_tag="VIF08_04275"
     CDS             3338..4216
                     /locus_tag="VIF08_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8135234.1"
                     /translation="MAQSGEVLIVGSINVDLVVRAAQLPAPGQTVTGGVFARHQGGKG
                     ANQAVAAARAGATVTMVGAVGDDEYGRGALADVVSEGVDVNRVAVMSGESTGLALIAV
                     DERGANQIVVAPGANAALGAAAVDTALAGYEPRAGSVAVLSFELGDEVIVAAGRYAAA
                     REMRLLVNPAPARALPRPLLAMSPIVLPNKGEAATLTGLSDPRAAAVALAGQTGAPVI
                     ITLGAGGALLHEPGGSPETYLLPAPTVEVVDTTGAGDAFVGALAAHLAAGRPLREAAA
                     AAVDAASKSVTSAGAR"
     gene            4218..>4858
                     /locus_tag="VIF08_04280"
     CDS             4218..>4858
                     /locus_tag="VIF08_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8135235.1"
                     /translation="MADLPEHVARNRAYWDAMADDWVAPGRRNWQQAEPHWGVWQIPE
                     SEAKLLPADLAGKDVIELGCGTAYVSAWLARRGARPVGIDNSAKQLETARAFQQEFGL
                     EFPLLHGNAESVPFPDASFDLAVSEYGAAIWADPYKWIPEAARVLRPGGQLIFLGNGV
                     FFMLTVPDTDAEGPSTDRLIRDYFGMYRFEWPEEDSVEFHLGYGDMIRLLRSSG"
BASE COUNT          743 a         1712 c         1709 g          694 t
ORIGIN      
        1 tcgtaagtca gttgcgtggc gccatcgagg tggaccgccg agaagggcac gatcccgtcg
       61 cccggcgcct gcgtctcctt gcccacggcg acgccgaaga gctcgttggc gaggtccccg
      121 gcaagccctg ggaactcggc gcccggagtg cggccgccga cgctcaggta tgaggtccgc
      181 ggcgcgaaat aggcacccgg cgtggcccgg tcgaggaact ccgtggccgc gtgacctgcg
      241 tgccgataac ggttggtcag gcggtagagg ccatgcggcg tgcccagggt cacgaggcag
      301 cccacggcct cagacacgtc ggcgatgcgg ccgttgaacg gcttgtcggc catggccagg
      361 cgcgcggcga tcccgcccgc cgagtggccc acgacgatga ccggcttgcg gccgccgtca
      421 cgccacgtcc tgccgatggc gcggccggtg cggagcatga ccgacccgaa gccaagcagt
      481 gctccgagca cccagtcggg cgtccacagc ggcgcgatgt cgacccgcgc cgcgccccgg
      541 gcgagcagcc ggcggcgcag cggccagtag ttgggcggca cggtcgcgaa gccgcccacg
      601 atgaggacgc tgggctgatc ggccgtggcc atcaggctag gaaaggaact cgatcttcag
      661 cggcgcgaag tcgtcggcca ggatgaagcc gaagacctgc gcatagaacg acgcctccgc
      721 ctcgagggag cggcggatgt tggccgcctg gcggaagcca tggccctcgc cctcgaatgc
      781 caggtaggcg tgcgggatgc gccgctcctt cagggcagcg acgatctgct cggactgcgc
      841 ctccggcacg accatatcgt cacggccctg gagcacgagc agcggcttgc tgaagccctc
      901 gacgtggtaa atgggcgacc gctcgtggta gagcgccatg gcctccgggt agggcccgac
      961 gagctggtcc atgtagcgtg actcgaactt gtgcgtctcg cgcgcgagcg cctcgatgtc
     1021 acccacgccg aagtaggagg cgccggcggc gaagacgtcg tggaacgcga gcgcgcacag
     1081 cgtggtgtaa ccgccggcac tgccgccgcg gatggccatg cgcgccgggt cgacatctgt
     1141 gcgttgcgcc aggtagcgcg cggcattgat gcagtcgtcg acgtcataga tgccccacgt
     1201 gccatagagc ttgcgcatgt actcgcggcc gtagccgttg gagccgcggt agttgacgtc
     1261 gacaaccgcg aagccgcggc tggtgaagaa ctgcttgtcc agatcgagca tcgccggcgc
     1321 gagggacgtc gggccgccat ggttgtggac gacgagtggc ggtcgtgcgt ctggcgggcc
     1381 ggccacgcct ggattcgttg gcgggtaata ccaggcatgg gcgacgtcgc cgtcgctcgt
     1441 cgggaaggtg atctcctcgg gcattgacac gtacgccgga tcaacggcga gctcgaacga
     1501 ccggcgcagg atgagcggct ccccggaatc gaggtcgagc agcacaacgc tgagcggctc
     1561 ggtggaacca gcggcctcga acgcgacgcg ccggccgttc acctggacca gtcccacggt
     1621 ggcgaacggc aaatcgatgc gcgccgccgc gcccctgacg gggaaccgcc acagctcgtc
     1681 cgtgccggcg cggcgcacca tggccacgat cgttccgtcg tcggcgaggc cataccacga
     1741 caacccaaag acccacagcg gcccaccgaa ctcggcgtcc atgggggcaa tcggccggat
     1801 ctcgtctgcg cggccggcgc cggtccagcg gtagaggttc caccagcccg atcgatcgga
     1861 aatgaagacg agcgcgccat cgggcgccca ttccggctga atgaccgatt catcgggccc
     1921 gccggcgatc agctgcggat cgaccggcag cccggccgcg tcgaggtggg cgacccacaa
     1981 ctcggtcgag tcccacggca tgttcgggtg gcgccacgac agccaggcca ggcgcgtgcc
     2041 gtcggggctg ggacgcggcg tgttgtagaa gtcgtatccc tgggcgagca cggtggtcgc
     2101 gccgctggcc ggatcgatgg caaccagggc gtcctccggc tccggtatgc gcccgcccgc
     2161 gcctgccgtg tgagcgtccg ccccagccgc cagcgcgcgc gtcatgtcct cgcgcacgca
     2221 cagcagccgg gcgcgcgcgc gatcaaaggt caggtcgccg tagcggtacg ggccatccgc
     2281 ggtcagcgcg accggcgctt cgccgcgcgg ctggcgatag atgcgcccgt cggcgaggtt
     2341 ggcgaagtag accgtaccgt cggcgacggc gtaccagccg ccgccgtact cgtgcgccat
     2401 cgtgcgcgcg ttgaattccg ggggcgtcac gtcgaccgcg ccgtcgcgct cgttccagcg
     2461 cacgatcacc tggcgcccgc gctcctgcgg ccggccctcc gtccagtaca ggtcgccacc
     2521 gtcgaaacgc ggctcacgca gcgtcaggct cgacgagaca gccatgtcca cggtgatcgg
     2581 cgacggccac gagccgtacg gcagcacctc gcgatcggcc aagtcgaacc ctcctgcgcc
     2641 cccggttgcc catgagcttg cttctcgtac cgactgtatc ctgccagccc atggccgaac
     2701 gcgacggcgc acctccaccc gagcagcgcc acaccggggt cgttcggcgc tctgatctga
     2761 cccggccgcg gccgtcgacg gtacgccagg actggctggc gcttgtgggc gtggccgcgc
     2821 tgatggtcat cgtcctggtc gtaatcgcca acttcttcgg cggcggcccg cccgctgacc
     2881 ccagttcgtc gccgacgatg gttgcgaacg catccgggac gccgaccttg aagccgacct
     2941 attcagccga gccgacgcgc accccgacgc cgacacccac gccttcgcca accccctccc
     3001 cgacggctac gcccagcccg acaccaactc cgacacccac gcccgcgtcg acgcctgcgc
     3061 cgacgcctgc gcccactgcg ccatcggccg ggttgatcat cctcgagccc gccgagggct
     3121 caaccgtgga tcgcgtcgtc aacgtcagcg gtttgaccca gcccggcgcc accatcaccc
     3181 gcgtcaagca gttctggttc gatgaccacg tgattgcgga cgaagcgggc cgctggacgc
     3241 tcgcggtcga gctcagtccc ggttggaatg caatcgagtt ccgcgtcgct gacgacccct
     3301 cgacgcaggt catcgtgaac ctctactacg caggctgatg gcacagtcgg gcgaggtcct
     3361 catcgtgggc tcgatcaatg tcgatctcgt ggtccgcgcg gcgcagctgc ccgcgcccgg
     3421 ccagacggtg acgggcggcg tgttcgcgcg gcaccagggt ggcaagggcg ccaaccaggc
     3481 cgtcgccgca gcacgcgccg gcgcgaccgt gaccatggtg ggcgccgtgg gcgacgacga
     3541 gtacggacgc ggtgcgctcg ccgacgtggt aagtgagggc gtcgacgtca accgcgttgc
     3601 ggtgatgagc ggtgaatcga ccggcctggc gctcatcgcg gtcgacgagc gcggcgccaa
     3661 ccagatcgtg gtggcaccgg gcgccaacgc agccctcgga gcagcggccg tcgacacggc
     3721 gctggccggc tacgaacccc gcgcgggcag cgtcgcggtg ctgagcttcg aacttggcga
     3781 tgaggtgatc gtggccgccg gccggtatgc ggccgcgcgc gagatgcgcc tcctggtcaa
     3841 cccggcgccg gcgcgcgctc tgccacggcc gcttctggcg atgtcgccta tcgtgctgcc
     3901 caataaaggt gaggccgcga ccctgaccgg gctgagcgat ccgcgcgccg ccgcggtagc
     3961 gctggcggga cagacgggcg cgccggtcat catcacgctg ggcgccggcg gcgccctgct
     4021 tcacgaacca ggcggctcgc cggagacata cctcctgccc gctcccaccg tggaggtggt
     4081 cgacaccacc ggcgccggcg acgcgttcgt cggtgcactg gctgcccatc tggccgcggg
     4141 caggcccttg cgtgaggctg cggccgcggc agtcgacgcc gcgtccaaat cggtcacctc
     4201 cgcgggagcg cggtgacatg gccgacctgc ccgagcacgt cgctcgcaac cgcgcgtact
     4261 gggatgcgat ggccgacgac tgggtcgcgc cgggccggcg caactggcag caggccgagc
     4321 cgcattgggg cgtctggcag atcccggagt cggaggcgaa gctgctgccc gccgatctcg
     4381 cgggcaagga tgtcatcgag ctcggctgtg gcacggccta cgtgtccgcc tggctggcgc
     4441 gccggggtgc gcgcccagtg ggcatcgaca actcagcgaa gcagctggag acggcgcggg
     4501 ccttccagca ggagttcggc cttgagtttc cgctgctgca cggcaacgcg gagagcgtgc
     4561 ccttcccgga cgcgagcttc gacctggcgg tcagcgaata cggcgctgcg atctgggccg
     4621 atccctacaa gtggatcccg gaggcggcgc gcgtcctccg acccggcggt cagctcatct
     4681 tcctgggcaa cggcgtgttc ttcatgctga ccgtgcccga cacggacgcg gaagggccgt
     4741 cgaccgatcg gctcatccgc gactacttcg gcatgtaccg cttcgaatgg cccgaggagg
     4801 acagcgtcga gttccacctg ggctacggcg acatgatccg gctgctgcgc tcgagcgg
//
LOCUS       DATBRX010000122        22204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2162754, whole genome shotgun sequence.
ACCESSION   DATBRX010000122 DATBRX010000000
VERSION     DATBRX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22204
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2162754"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VIF08_04285"
     CDS             complement(<1..755)
                     /locus_tag="VIF08_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY8135236.1"
                     /translation="MSTHSIPAERYAARLARAQAALPGEDAYALLIGVGADLQYLTGY
                     GAHELERLTMLVVPAMGRATLIAPRLELAPAQACTAAQAGLIDLAPWEETDDPFAIVA
                     ARLEASDSRPEIQLGALGGAWGRLGGLLVSDRLWATFLLRLQATLPDAAFGLASTVLA
                     PLRATKDAEEIELLRTAALAADRVVEQIAAGRLVGRTEADVSKEVLDRLVKEGHDEGS
                     FAIVASGPNSASPHHGPGPRVIEAGEPIVLDIGG"
     gene            complement(752..1090)
                     /locus_tag="VIF08_04290"
     CDS             complement(752..1090)
                     /locus_tag="VIF08_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135237.1"
                     /translation="MNVRVVADDLIWASRLDAAVKRAGAQPVRDGGDAVIVDLNGRGY
                     DGIAAVAAAVESGRTVIAVGQHDDPATRKRALTAGARRVFSYSKMFSDGPQVVADLVA
                     GRFDAPGVSA"
     gene            1117..2091
                     /locus_tag="VIF08_04295"
     CDS             1117..2091
                     /locus_tag="VIF08_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8135238.1"
                     /translation="MDRTRLVGIALVVVSAAGFGSGALFAKLGIYPNGVDWMTLLSWR
                     FVFAALLSWGWLLLWPSQRRALRSLSRRRVLVLLALGIFYMGNTGTYYAGLETVDASL
                     AALIVYIYPTVVAVISIRFARRLQGRRAWGALALATVGIALALGGINPDHAPPLLGLI
                     VMIGSPLIYAVWIVLAARMSGERRESDDAQTAPPYDSETTASAEPTDSAPTAAIMLSA
                     TAVGWTAAAVIFGRPVLPADVPMDAWWALFGIGLLATAVAMQAFYAGARRIGAAQASL
                     VSTVEPVYTIALAALLLHETLTPVQILGGIMVIIGVIVAQTGQQRRTA"
     gene            2088..2984
                     /locus_tag="VIF08_04300"
     CDS             2088..2984
                     /locus_tag="VIF08_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8135239.1"
                     /translation="MKLVSYRTPSAFAGEPGRDARLGIVEGESVVATASLGHGVPGTM
                     ADLLAAWPDALGVLQAAYRAADQPETAAVNAVELLAPVPRPGKIVAAGVNYATHAAEG
                     NRAAPDHPVLFAKLPTSVIGQGAEIRWSPELTQAVDFEAELAVVIGRTCRRTDESEAL
                     AYVAGYTCLNDVSARDLQYSDKQFVRAKSLDTFCPMGPWLVTADEIADPQALRVRCLV
                     NGEVMQDASTAEMIFGVAALVSFCSQAFTLEPGDVIATGTPSGVGWFRDPKRMLRDGD
                     EVVIDIEHVGRLVNRCREERPD"
     gene            2999..5470
                     /locus_tag="VIF08_04305"
     CDS             2999..5470
                     /locus_tag="VIF08_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8135240.1"
                     /translation="MPELPTGTVTFLFTDIEGSTRLLDELGAEAYAEALAGHRRIVRD
                     AIARHGGVEVDTQGDAFFVAFPEASAAVTAAGEAQRGLADHRVRVRMGLHTGTARVSD
                     GGYIGSDVHLGARVAAAGHGGQVLMSSATRGLIAYDVLDLGQHRLKDFDSPLAIYQLG
                     HDRYPPLRTISNTNLPRPASPLVGRTREVAEVTARLQNGARLLTLTGPGGMGKTRLAI
                     ESASELVSHNRNGVFWVPLASLTDPALVMSEVGQTLGAKGSVAEHVGASEMLLLLDNF
                     EQVVDAAPELAELLQACANLRLLVTSRERLRVAGEVEYAVPPLAEPEAVELFTARTGI
                     ADDGDVAELCRRLDNLPLAVELAAARASVLSPAQMLERLGQRLDLLKGGRDADARQQT
                     LRATIEWSHDLLNAEEQALFARLAVFRGGCALEAAEEVIEADVDTLQSLVDKSLVRHS
                     NGRFWMLETIREFAAERLDESGMAETMRRSHAVHYLALAEEVESALVGPGSDRWHDLI
                     QRDHENIRSALDHFEATSEIESAMRLSGAIAEFWDQRAHHPEALLRYTRLLEADTRPT
                     PARAKALDAASMIATKSGHQQTAVKWQEEALALHRQFGDKRGTAIAMWGLGYLWVEDG
                     NYVAAQDILRESVDLLGEVGDDVAAAWATRTLAFSYFKPGDNARARPLYEETLRRARE
                     IGDADLEAHGLGALAEYAIDDGRPLEAALLARQTLQIVAGAADPLLVTSRLSGVARVL
                     SLLGRPTVAARLISHAEFRHEEIGAREIWVATENQKTISLIHAALDEATFAREWDEGR
                     KLSPEAAMALAIAELDDAVERNRAN"
     gene            5460..6125
                     /locus_tag="VIF08_04310"
     CDS             5460..6125
                     /locus_tag="VIF08_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8135241.1"
                     /translation="MPTDSLTLLVVDDEPAWVGALGAVLGKAGHHIVAAYNGEEALRR
                     FRAERVDAVLLDLAMPDMDGAAVARQIRAESDVPILIVSGERDPAAPAELLDLGADDY
                     IRKSMPNDEKLARIRAAVRRRGRAATGSGWAVDARRHEIAWRGQVVPATAIEFRLLES
                     LVTHIGELRSHAQLLAAGWPDVRDPDPLWLKPHLARLRDKLARLGAPAPTAVRAVGYR
                     LDA"
     gene            complement(6107..7342)
                     /locus_tag="VIF08_04315"
     CDS             complement(6107..7342)
                     /locus_tag="VIF08_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8135242.1"
                     /translation="MIARSLSRLSPYGRELPAEFWFLWLGTVINRLGGFAVPFLMLYL
                     TAKRQMDPASAALFVSVLGAGSFVAQLVGGEVADRFGRRPVLLFSLFAAPVAMLSLGV
                     VREPWLIAVAMFALGFLTDLFRPAMSAAVVDLVPANRRTRAFGYIYWAINLGAAAAPI
                     VAGLMANYDYFLLFLGDAVTTAVFGVIVFLRVPETQSADHARDARAANTRGRAGQALR
                     DPMLLFFFVLSLAVGVMYSQSHVTLPLDMAAAGLPPSDYGLAIAVNGALIVLITLQVT
                     RFLERFPRYLVMALAALLLGVGFGLTELASTLPLYALTVVIWTLGEVIGAAVAPVIVS
                     EMSPAPLRGLYQGIWGASWGLSFFIGPALGGFVLHNYGSGMLWGGVFVLGIVVSFGYL
                     VMSIPARRRAQQVAQASSR"
     gene            7441..7698
                     /locus_tag="VIF08_04320"
     CDS             7441..7698
                     /locus_tag="VIF08_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135243.1"
                     /translation="MSDYSVSQFSSRESAGPVTCAACGCRLVGLTGLLDGAYRHFPSL
                     KSGHDARGCRTACVDALHDHAGRPLASSVADGQSALDSAAA"
     gene            complement(7860..9119)
                     /locus_tag="VIF08_04325"
     CDS             complement(7860..9119)
                     /locus_tag="VIF08_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8135244.1"
                     /translation="MATGIQRARGAPKRGARRLAELEFLAEVARLVGSARTWDELMGT
                     IVDRATAAAQAEVCSLYLLDRDGLGVTLAATNGLDPEQIGLARLPLGVGITGIVAQTR
                     EPQTALDVHADERFHWIRGVDQDKFTSMCSVPLIWNDQVVGVLNVQTERRRRFTRQDV
                     AFLEALAGLLAGIVERNRLQREAEAQLESLRAIDEARAHLVAVVTHALRTPLAVVRAY
                     VELLGGATKRMAGRDEASEASAWERSALEQVDRLDQTVDSILASLRVLTPDSGPVVAL
                     DVASVVDDMAREMAPMFRRQKLAASFVERPLRAYASVDQLKRLLGYLLENAAKYAPAE
                     GTIDIYGWRKDGRTYLAITDDGPGIPAEWRERIFEPFVRLDDSPRGSGIGLFAARHLA
                     RSMGGELLAEDRAPKGSQFVLELPAAR"
     gene            9163..10329
                     /gene="hppD"
                     /locus_tag="VIF08_04330"
     CDS             9163..10329
                     /gene="hppD"
                     /locus_tag="VIF08_04330"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY8135245.1"
                     /translation="MTDTLTRGAANQAGAHINKDAYAQAQDFLPIKGIDYVEFWVGNA
                     RQAAAYYRALWGFTNVAYAGLETKIRDRASYVMVQNDIRFVFTAPLTPDGEIAEHVRK
                     HGDGVHDIAFAVDDVKSAWTETTKRGAKSYLEYAESSDDDGTLRRSAVHTYGEVIHSF
                     VDRSDYHGAFAPGYRKVKKPARAATGLSLLEVDHCVGNVELGDMNKYVDYYRDTFGFS
                     QLIHYDDKVIHTEYSALMSKVMTNGNGRIKFPINEPASGKKKSQIQEFLDWYLDAGTQ
                     HVALRTEDIVGTVRALRESGVEFLGMPHEYYAALPDRVGDVGVPIDVLEELGIEADRD
                     EEGYLLQIFTRPVQDRPTFFFEIIERHGSRGFGVGNFKALFEAIEREQALRGNL"
     gene            10330..10806
                     /locus_tag="VIF08_04335"
     CDS             10330..10806
                     /locus_tag="VIF08_04335"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEY8135246.1"
                     /translation="MSNVRWARIGFALLAWLLAGLAIVQIYLAGLALFVVDSTFEMHR
                     NFGYLIGIIALVQLVLAFAGKLGGRMIGTSALLLGLMALQSVLVIMRADQPTIAAIHP
                     VNGFLIVLLSVWIAWRALGYIRAPLPPEPVEAAPAPAATHSPRPTLDQQDEQEDRL"
     gene            complement(10859..11680)
                     /locus_tag="VIF08_04340"
     CDS             complement(10859..11680)
                     /locus_tag="VIF08_04340"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEY8135247.1"
                     /translation="MKRSTRLSELHPALLRLRTAQLVAARRLADGIRRPHFARVQLSS
                     SLPVTLVKHESRLRRRLAGLDPRYQETKIVNLALAARSLDGLLVHPGELFSFWDRVGN
                     PTERRGFAPGLVLRHGEVGIDVGGGLCQMSNLLYWMALHTPLAVTEQHHHGFDPFPDD
                     VRVQPFGSGASVFYNYGDLRLLNTTGATFQISVQLTDTYLLGAIRSDRELPTQHEVEE
                     RGHRFRRAEDGRVYRENELWQTVIDRATGAHLVSRLITRNHALVKYPVPDNLIEA"
     gene            complement(11728..12153)
                     /locus_tag="VIF08_04345"
     CDS             complement(11728..12153)
                     /locus_tag="VIF08_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY8135248.1"
                     /translation="MSDWNNKIIDEFRAKGGRGVGPFGDGLLLLTTRGARSGKAHTTP
                     LAFHRDRGRYVVIASMGGAPKHPAWYHNLLANPEAEVEVGTEKFRVNATPQPSGPERD
                     RLFAQQATIMPGFWEYEKKTTRIIPAVVLEPIEQAAAAA"
     gene            12225..12809
                     /locus_tag="VIF08_04350"
     CDS             12225..12809
                     /locus_tag="VIF08_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8135249.1"
                     /translation="MVIEKTPASCRGRRRDDTIDARVLGITNRHLAERGFAALSISAI
                     ADEACTTRQALYRRWPTKERLVADAIRIAGGSDAVCVSDDPRADLEHELAAWVQDDTT
                     AGLAMAGAMLERETPEDARDCYREHVLAPRQQRMTDILVHAQALKRIDVDADVAGAVG
                     VAIGAAYVAHLSGSIAPDWPARTAALVWRALGGK"
     gene            12806..13813
                     /locus_tag="VIF08_04355"
     CDS             12806..13813
                     /locus_tag="VIF08_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8135250.1"
                     /translation="MKIAHARERHAPAGASFRLMAALDEAGRQWLDLEVARRRAVNAD
                     ERLAHNEVLFRQPVTTLDDHLARGLRVAALGALLDVYEPRGADDDVVLDGTDLSFGPP
                     ILRPASVRDFYAFEKHVGTIWSRRGQEIPEAWFRLPIFYFSNVSEVRGPDQPVWPPRG
                     SSELDYEMEIAALIDTPAQNLDAARGEEAIGGFMIMNDWSARDLQRDETAVRLGPAKG
                     KDFATTFGPWLVTPDELADKRSGKAYDLACTAEVNGTILSRSNLGTIHHSFGAMIERA
                     SADVRLVPGDVIGSGTVGGGCLLEIKDESGFGRYLQPGDRVTLKVERLGELSAPIIQR
                     P"
     gene            13925..14842
                     /locus_tag="VIF08_04360"
     CDS             13925..14842
                     /locus_tag="VIF08_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8135251.1"
                     /translation="MYLLLGIALAGFLAQLIDGSLGMGYGVSSTTLLLTVGLTPALAS
                     ASVHLAEIGTTAVSGASHWRLGNVDMKIMALLAIPGGVGAFLGAVVLSNLSTEAAKPW
                     VSVVLLVLGAVIVFRFLHARRTRQAPAVLHDQPLNAAPRRSRRWMLTPLGVAGGFLDA
                     SGGGGWGPVVTSTLMASGRLKPRTIIGTVSMSEFVVAIAASVGFLLALGAAGIRWDIV
                     AMMLVGGSIAAPVAAWLVRKFDDRDLGTAVGAIIILLNIDRLLVLLGMDSSLVTAVRL
                     FAIASAAVIIGMLVWRGRAGAVGGATASA"
     gene            14870..16093
                     /locus_tag="VIF08_04365"
     CDS             14870..16093
                     /locus_tag="VIF08_04365"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY8135252.1"
                     /translation="MFYVSRGNVPHTRHTQHRAPDGSLYAEELFGMEGFTGRSSLLYH
                     RVPPTQTHQIERVSEVHKEAIDDGVHRHRLVKTGGLPAAGNVVEGRMPLFFNSDVLMG
                     IVRPTENLAANIFYRNGEADEMLFVHTGEGVFESNFGPMRYGPGDYIVIPIGTTWRLN
                     QDAKSDHRILYVESPSEIVPPKRYRNDWGQLLEHAPYSQRDIRVPDDVAVHDEAGDFV
                     IWVKVRGRITAYHYRHHPFDVVGWDGYLFPFIFNIGDFQPITGRVHQPPPVHQTFAGR
                     NYVVCSFVPRKFDYHPLAIPAPYNHSNINSDEVIYYVAGNFMSRRGVDISSFTLHPAG
                     IPHGPHPGTVEASIGKEATEELAVMIDTFHPLNMTREAARLEDASYAYSWLPPEDGGE
                     HARELSERGPEAFPD"
     gene            16179..16730
                     /locus_tag="VIF08_04370"
     CDS             16179..16730
                     /locus_tag="VIF08_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8135253.1"
                     /translation="MDPAVVERARGGDREAFTTLVGQVSDSLYAVAFRILRETASAED
                     ALQNALISTWQQLPHLRDVERFEAWSQRILVHACYAEFSRRRQFAANVRVIDLNRAGA
                     SDGSRVLADRDELDRAFRKIPVDQRAVFVMHHYVGLPLVEIAETLGIPAGTARSRLHN
                     ATRALRTVMAAGALRTLEEGRPA"
     gene            16727..18505
                     /locus_tag="VIF08_04375"
     CDS             16727..18505
                     /locus_tag="VIF08_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135254.1"
                     /translation="MSDDRMFERSAQAWLELGPSTAPKRVVDAALRTIATTPQQRDLQ
                     ASWRFPRLTIQMRFAVAAAVGLMVLGAGLLVLRSPQNQIGQTPTPSPSASPITMERLQ
                     GAWASVGTRIDPILGNAPDKSGLQTIAVDAASLRLNGWKGEIASSASVIGAAGQLELL
                     MDAPQESLNREHWDCTTGDSGTYYASLSPDDQTLTLTSASDECASRDEILTGDWTRWS
                     CPDPARYAYCSHVSELAPGRYTAANFKPFEGGTRGQFAYTVPAGWADFHFPPTWPGPD
                     RRADLTLATVANPDSPLINVDSTIRASTMSAVIGAPPLACPDPVTASGGPAASVADWL
                     GSRPFLAVSQRRPITIGGLSGVQIDVSVVAGFVDPCTLGSAPGAPIEYSTQIEIFGGS
                     ETGDSVTMDTATDDHARFIVLDTGDWQGYPGTLLISLTAQGQSALDVVIAQAMPVVQT
                     FEFIPGPSASPTAVSSPIAVNATDLQGTWNSVGTRPIPDALDQLTVFTLGANYLDIHQ
                     SDGDVRSSWVLIEDTINRVWVHGPLTTVPVAPRWDCFGIGEYDVSLSTDGATLTFTPI
                     DEDCAQREAILAGDWTRDVAAEPSLK"
     gene            18661..19788
                     /locus_tag="VIF08_04380"
     CDS             18661..19788
                     /locus_tag="VIF08_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEY8135255.1"
                     /translation="MPTAKPTASPSPVPTPGLFRAPVVLDQAMLPIVVADFDGDGNLD
                     IAGGAADARVQLLLGVGDGRFQPGAEMPGGNGPASIGAADFNGDGRLDIVMAYSGVSD
                     QGTGDDDILVFLAKDDGSFEALVQPAGVNPQALVVGDFNEDEVPDLATANGFDYLSIF
                     AGLGDGTFADPENYPINARFASGIAAADFDGDGNLDLVTTNSMIGKGRSDRTMSVLLG
                     AGDGTFGEPQTFPVSGPQPIMPVVGDFNGDGFADVASPDGYPTRVTSVLLGLGNGALR
                     ETSYYLCGPNPHTLATADLDGDGILDLVCGNAGEEGGPVGKGIAALFGLGDGTFKPGV
                     DLVGNQFGEGIAAAADLDNDGQIDLIVYSDTLVVLFNDIPR"
     gene            19817..20293
                     /locus_tag="VIF08_04385"
     CDS             19817..20293
                     /locus_tag="VIF08_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8135256.1"
                     /translation="MDANDLVQMLEDVEGRLIEDAAGPAPSGLTQPDEGGTERWEAAQ
                     VWAHMAEFVGYWQAQLEHVVDSYAGQPVPFGRTKEDPGRIEGIETGRRVPIAQLMQQV
                     HDGIEVTRRYLPTLSAAQWASVGLHSRRGEMTVPQMVMRFTIDHLEEHADQLDDLA"
     gene            20290..20943
                     /locus_tag="VIF08_04390"
     CDS             20290..20943
                     /locus_tag="VIF08_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135257.1"
                     /translation="MKVYLETGEKKSFATAIDWIGLARGGKTPDDAMAALVEYTSRYL
                     ESMGAAAAALKAPATVAGLNIVERLPGNKTTDFGAPDAILPADRKPLSQRDLDAAIES
                     LHAAWAAFATAAKRAGGKDLAPSGPRGGGRSLDKMTAHVHEAQGGYTYAVGGKSKAGA
                     SWSEIQRDFIGALRLRNAGALPDFGPRGGERWPALFAIRRSAWHALDHAWEIEDRLG"
     gene            complement(20940..21509)
                     /locus_tag="VIF08_04395"
     CDS             complement(20940..21509)
                     /locus_tag="VIF08_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135258.1"
                     /translation="MTRLITRLYPRAWRDRYGAELSDLIDETGMTPRVAIDVARGAAR
                     EWTNQARLGLAGGGSVVIGPAWRHPQMWAAMALIILAPTAAFVLLSILAYQLGATWLI
                     TFMEPVTRWMQTVRIIDLMLVVSPVIALLIAAAPLVRVELHREATGREAVVGVRLKAA
                     NVLIGLLALFIAVLLVGHILAESVLRVGA"
     gene            complement(21506..21808)
                     /locus_tag="VIF08_04400"
     CDS             complement(21506..21808)
                     /locus_tag="VIF08_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8135259.1"
                     /translation="MADIQATIDPGLMLMISLAAGPKHGHAMLLDVERFAGVRLGPGT
                     LYGALSRLEASGHVEPLPAEGRRRPYRLTGAGKAALEQRLGSLQRAVATGLARVSA"
     gene            complement(21863..>22204)
                     /locus_tag="VIF08_04405"
     CDS             complement(21863..>22204)
                     /locus_tag="VIF08_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY8135260.1"
                     /translation="REEVDRSYIELMERLLTDGNYPALATHDDRLIDHALAFVKEHAI
                     DPARYEFQMLYGVRRDLHERLVALGQTVRIYVPYGSQWYPYYMRRLGERPQNVISILL
                     SVLQEGRGKKA"
BASE COUNT         3658 a         7590 c         7407 g         3549 t
ORIGIN      
        1 ccgccgatgt cgagcacgat cggctcaccc gcctcgatca cccgtggccc gggaccgtgg
       61 tggggcgagg ccgagttggg gccgctcgcc acgatcgcga acgagccctc gtcgtgaccc
      121 tccttgacga gccgatcgag cacttccttg gacacgtctg cctccgtccg gccgactaac
      181 cgcccagccg cgatctgctc caccacgcga tcggcggcga gcgccgccgt gcgcaggagc
      241 tcgatctcct cggcgtcctt ggtcgcacga agcggagcga ggaccgtcga agccagtccg
      301 aatgcggcgt cgggcagcgt cgcctggagt cggagcagga aggtcgccca cagtcgatcc
      361 gaaacgagca gccctcccaa gcggccccac gcgccgccca gcgcacccag ctggatctcc
      421 ggccggctgt cgctcgcctc gagccgcgcg gcaacgatgg cgaagggatc gtcggtctcc
      481 tcccacggcg cgaggtcgat caggccggcc tgcgcggcgg tgcaggcctg ggccggagcc
      541 agctcgagac ggggcgcgat cagcgtggcc cgtcccattg caggcacgac gagcatggtc
      601 aggcgctcga gctcgtgcgc gccgtagccg gtcaggtact gcaggtccgc gccgacgccg
      661 atcagcagcg cgtacgcgtc ctcgccgggc agcgctgcct gggcgcgcgc cagccgcgcg
      721 gcgtagcgct ccgcggggat cgaatgagtg ctcatgccga aacgccaggc gcgtcgaagc
      781 gcccggctac caggtcggcc acgacctgcg ggccgtcgct gaacatcttg ctgtaggaaa
      841 agacgcgtcg cgctccggcg gtcagcgcgc gcttgcgggt tgcagggtca tcgtgctgcc
      901 cgaccgcgat caccgtccgc ccgctctcta cggccgctgc gaccgcggcg ataccgtcat
      961 acccgcgccc attcagatcg acgatgaccg cgtctccacc gtcgcgcacg ggctgcgccc
     1021 cggcgcgctt cacggccgca tccaggcgcg acgcccagat caggtcgtcg gcaaccaccc
     1081 gcacgttcac gggccgcagt ctaccctcgg acgctcatgg accgaactcg gctcgtcggc
     1141 atcgcgctgg tcgtcgtgtc ggccgccggc tttggctcgg gtgcgctgtt cgcgaagctg
     1201 ggcatctacc ccaacggggt cgactggatg acgttgctgt cgtggcgctt cgtcttcgcc
     1261 gcgctgctgt cgtggggctg gctcctgctc tggccgagcc agcgccgcgc cttgcgttcg
     1321 ctgtcgcgcc gccgggtcct cgtgctgctc gcgctgggca tcttctacat gggcaacacc
     1381 ggcacgtact acgccgggct cgagacggtc gacgcatcgc tcgcagcgct gatcgtctac
     1441 atctacccga ccgtcgtcgc ggtcatctcg atccgcttcg cgcgccggct ccagggacgc
     1501 cgggcctggg gcgccctcgc gctggccacg gtgggcatcg cactggcgct gggtggcatc
     1561 aaccctgacc acgcaccgcc cctgctgggt ctgatcgtca tgatcggctc gccactcatc
     1621 tacgcggtct ggatcgtcct tgccgcgcgc atgtccggcg agcgccgcga gagcgacgac
     1681 gcgcagaccg cgccaccgta tgactcggag acgacggcct ccgctgagcc caccgattcg
     1741 gcgccgacgg cggccatcat gctgtccgcg accgccgtgg gctggacggc tgcggcggtg
     1801 atcttcgggc ggccggtcct gccggccgac gtgccgatgg acgcctggtg ggccctgttc
     1861 ggcattggcc tgctggcaac tgcagtggcg atgcaggcct tctacgccgg tgcgcgacga
     1921 atcggcgccg cgcaggcgtc gctggtcagc accgtcgagc cggtgtacac gatcgcgctc
     1981 gcggcactgc tcctgcacga gacgctgacg cccgtccaga tcctgggcgg catcatggtc
     2041 atcattggcg tgatcgtggc tcagaccgga cagcagaggc gcacggcgtg aagctcgtct
     2101 cgtatcgcac accgtcggcg ttcgccggcg agcccggccg tgacgcgcga ctgggcatcg
     2161 tcgagggcga gtcggtggtt gcgacggctt cgcttggcca cggggtcccg ggcacgatgg
     2221 ccgatctgct ggcggcctgg cccgatgcgc tcggcgtgct ccaggccgct taccgcgctg
     2281 ccgatcagcc ggagaccgcc gccgtcaatg cggtggagtt gctggcaccc gttccgcgac
     2341 cgggcaagat cgtcgcggcc ggtgtgaact acgccaccca cgccgcggag ggcaaccggg
     2401 ccgcgcccga ccaccccgtc ctcttcgcga agctgcccac gtcggtcatt ggccagggcg
     2461 ccgagatccg ctggtcaccg gagctcacgc aggcggtgga tttcgaggcg gagctggcag
     2521 tggtcatcgg tcggacgtgt cggcggaccg atgaatcgga ggccctcgcc tatgtggccg
     2581 gctacacctg cctgaacgat gtgagcgcgc gcgatcttca gtacagcgac aagcagttcg
     2641 tgcgcgccaa gtcgctcgac acgttctgcc caatgggtcc gtggctggtc acggccgacg
     2701 agatcgccga tccgcaggcg ctgcgtgtcc gctgccttgt caacggcgag gtcatgcagg
     2761 acgcctcgac cgcggagatg atcttcggcg tcgcggccct ggtgagcttc tgctcgcagg
     2821 cgttcaccct ggagccgggc gacgtgatcg cgaccggcac gccgtcgggc gtcggctggt
     2881 tccgtgaccc caagcgcatg ctcagggatg gcgacgaggt ggtgatcgac atcgagcatg
     2941 tcgggcggct cgtcaaccgc tgccgcgagg agcgcccgga ctgatacttg gcggtgcgat
     3001 gcctgagctg ccgacaggaa ccgtcacctt tctcttcacc gacatcgagg gctcgacgcg
     3061 cctgctcgat gagctgggtg ccgaggcata cgccgaggcg ctcgccggac accgccgaat
     3121 cgtgcgcgac gccatcgcgc gccacggggg cgtcgaagtc gacacccagg gcgatgcgtt
     3181 cttcgtcgcc ttccccgaag caagcgccgc ggtgacggcc gctggtgagg cacagcgtgg
     3241 tctggccgac caccgcgttc gagtccgcat gggcctgcac accgggacgg cccgagtgtc
     3301 cgacggcggg tatatcggca gtgatgttca cctgggagct cgcgtcgcag cggcaggcca
     3361 tggcggtcag gtgctcatgt ccagcgccac ccgtggactg atcgcctacg acgtcctgga
     3421 cctcggccag caccgactga aggacttcga cagcccgctg gcgatctacc agctcggcca
     3481 tgaccgatac ccacctttgc ggacgatctc caatacgaac ctgccgcggc cggcgagccc
     3541 gctggtgggc aggacacgcg aggtcgccga ggtgaccgcc cgcctgcaga acggcgcgcg
     3601 actgctgacc ctgaccggac caggcgggat gggcaagaca cgcctcgcaa tcgagtccgc
     3661 gtccgagctc gtgtcccaca accgcaacgg cgtgttctgg gtgccgctgg ccagcttgac
     3721 cgacccggct ttggtcatga gcgaggtcgg ccagaccctg ggcgccaaag ggtcggtcgc
     3781 cgagcacgtg ggtgcgagcg agatgttgct gctgctcgac aacttcgagc aggtcgtcga
     3841 tgcggcgccg gaactggccg agctgctgca ggcgtgcgcc aacctgcggc tgctcgtcac
     3901 gagtcgcgag cggctgcgcg tcgcgggcga ggtcgagtac gccgttcccc cgctcgccga
     3961 gccagaggcc gtcgagctgt tcaccgctcg cacgggaatc gccgacgacg gcgacgttgc
     4021 cgagctttgc cggcggctgg acaacctgcc gctcgcggtt gagctcgccg cggcgcgcgc
     4081 gagcgtcctc tcgccggcgc agatgctgga gcggctcggc cagcggttgg acctgttgaa
     4141 gggcggccgt gatgccgacg cgcgccagca gacgctgcgc gccacgatcg aatggagcca
     4201 cgacctcctg aacgcggagg agcaggcgct gtttgcccgc ctcgccgtct tccgcggcgg
     4261 ctgcgcgctc gaagccgcgg aggaggtgat cgaagctgat gtcgacacgc tccaatcgct
     4321 cgttgacaag agccttgtcc gccattcgaa cggccgcttc tggatgctcg agacgatccg
     4381 cgagttcgcg gctgagcggc tcgacgagtc cgggatggcc gaaacgatgc gtcgcagcca
     4441 tgccgttcac tatcttgcgc tggccgagga agtcgaatcc gcccttgtcg gcccgggatc
     4501 agaccggtgg cacgacctga tccagcgcga ccacgagaac atcagatcgg cgcttgacca
     4561 tttcgaggcg acctcggaaa tcgagtcggc aatgaggctc tccggcgcca ttgctgagtt
     4621 ctgggaccaa cgggcccacc accccgaggc gctgctccgt tacacgcgct tgcttgaggc
     4681 agatactcgg ccgacgcccg ctcgggccaa ggcgctcgac gctgcttcca tgattgccac
     4741 caaatccggc caccagcaga cggccgtcaa gtggcaggaa gaagcgctgg ccctgcaccg
     4801 ccaatttgga gataagcgcg ggactgcaat cgcgatgtgg ggcctgggtt acctgtgggt
     4861 tgaagacggc aattacgtcg cggcgcagga catactccgc gagagcgtcg acttactggg
     4921 ggaggtgggt gacgatgtcg cggctgcgtg ggcgacgagg accctcgcgt tcagctattt
     4981 caagcccggt gacaacgccc gggcgcgacc gctctacgaa gaaaccctgc gtcgtgctcg
     5041 cgaaatcggc gatgccgacc tcgaggcaca cggtcttggt gcgttggcgg agtacgcaat
     5101 cgacgatgga cggccgctgg aggcggcact tcttgctcgt caaaccctgc agatcgtggc
     5161 cggcgcggcc gatccgctgc tggtcacctc gcgcttgagc ggcgtggcca gggtcctcag
     5221 cttgctgggt cgcccgaccg tcgcggcgcg actgatttct cacgccgagt tccgccacga
     5281 ggaaatcggc gcacgtgaga tctgggtcgc gacagagaac cagaagacga tctcattgat
     5341 ccacgcggcc ctcgacgagg ctacgttcgc acgggagtgg gacgagggac gaaagttgag
     5401 cccagaggcc gcgatggcgc tagctattgc ggagctcgac gatgccgtcg agcgaaaccg
     5461 tgccaactga ctctctgacg ctgcttgtcg ttgacgacga gccggcgtgg gtcggcgcgc
     5521 tgggcgcagt gctgggcaag gcggggcatc acatcgtggc cgcgtacaac ggcgaggagg
     5581 cactgcgtcg tttccgggca gagcgagtcg acgccgtgct gctggacctc gcgatgcccg
     5641 acatggatgg cgccgccgtc gcgcgccaga tccgcgccga gtcggatgtg cccatcctga
     5701 tcgtgagcgg cgagcgcgac cccgctgccc cagccgagct gctcgacctg ggcgccgacg
     5761 actacatccg taagtccatg cccaacgacg agaagctggc ccgcatccgg gcggcggttc
     5821 gaaggcgcgg gcgggccgcc acgggcagcg gctgggcggt cgacgcgcgg cgccacgaga
     5881 tcgcatggcg cggccaggtc gtgcccgcga cggccatcga gttccggctt ttggagtcgc
     5941 tcgtcaccca catcggcgag ctgcgctcgc acgcccagct cctggccgcg ggctggccgg
     6001 atgtacgcga ccccgaccct ttgtggctga aaccgcacct cgcccggctg cgcgacaagc
     6061 tcgcccgcct gggtgcgccc gcgccgaccg cggtccgggc ggtgggctac cggctggacg
     6121 cctgagcgac ctgctgggcg cgccgccggg cggggatcga catcaccaga tagccgaagc
     6181 tcaccacgat cccgagcacg aacaccccgc cccacaacat gccggagccg tagttgtgca
     6241 gcacgaaacc gccgagcgcc ggaccgatga agaacgacag cccccacgac gcgccccaga
     6301 tcccctggta caggccacgc agcggagcgg gtgacatctc actgacgatc acgggcgcca
     6361 cagccgcgcc gatgacctcg ccgagcgtcc agatcaccac cgtcagggcg tacagcggca
     6421 atgtcgacgc gagctccgtc agcccgaagc ccacccccag cagcaacgcc gccagggcca
     6481 tgacgagata ccgcggaaag cgctcgagga agcgcgtcac ctggagggta atgagcacga
     6541 tcagcgcgcc gttgaccgcg atggccagcc cgtaatccga cggcggcagg ccggccgcgg
     6601 ccatgtccag cggcagcgtc acgtgcgact gtgagtacat gaccccaacc gccagcgaca
     6661 ggacgaagaa gaagagcagc atcgggtcgc gcagagcctg ccccgcccgg ccgcgggtat
     6721 tggcagcgcg ggcgtcgcgc gcgtgatcgg ccgactgggt ctccggcacc cgcagaaaga
     6781 cgatcacgcc gaatacggcc gtcgtcaccg cgtcgcccag gaacagcagg aagtagtcgt
     6841 agttggccat gaggcccgcg acgatgggtg cggccgcggc gcccaggttg atcgcccagt
     6901 agatgtagcc gaacgcccgc gtgcggcgat tggcggggac gagatcgacg accgccgcgc
     6961 tcatggccgg acggaagaga tcggtcagga agcccagcgc gaacatcgcg accgcgatga
     7021 gccacggctc gcggacgacg cccaggctca gcatcgcgac cggtgccgcg aacagagaga
     7081 acaggagcac gggcctccga ccgaaacgat cggccacctc gccacccacg agctgggcga
     7141 cgaaggagcc ggcacccagc accgacacga acagtgctgc gctcgcagga tccatctgcc
     7201 gcttcgctgt caggtagagc atcaggaagg gcacggcgaa gccgccgagg cggttgatga
     7261 ccgtgcccag ccacaggaac cagaactcgg ccggcagctc gcgcccgtag ggcgagagtc
     7321 gtgaaaggga gcgcgcgatc aaggccggcg cacgatacca gcgggctcgc aaccggaatg
     7381 caacaaaagg gataccgcgc cgccgctgac cggggcaccg ccggtaagta gcgtgggtca
     7441 atgagcgatt actcggtgag ccagttttct tcgcgtgagt cggccggacc ggtcacgtgt
     7501 gccgcgtgtg gctgccgcct cgtcgggctg acgggcctgc tcgatggcgc ctaccgccac
     7561 ttcccgtcgc tgaagtcggg ccacgatgcg cgcggctgcc gcacggcgtg cgtcgacgcg
     7621 ctccatgacc acgccggccg gccgttggcg agcagcgtgg ccgacggtca gtcggcgctg
     7681 gattcggccg cggcctgacg gccggtaccc gtggcggccg ggccgccagg gcaggttcac
     7741 atgatcgaga ttgctgtcat ttccatcttg gtagtcggcg ccttcgcctt catcggctgg
     7801 tggcgcggcc cggacatccc gagctgacct cttagggctg gcccctggcg cgcgggatgc
     7861 taccgcgcgg cgggcagctc gagcacgaac tggctgccct tgggggcgcg gtcctcagcc
     7921 agcagctcgc cgcccatcga gcgcgccagg tgtcgcgccg cgaacaggcc gatcccgctg
     7981 ccacgcggtg aatcgtccag gcgcacgaat ggctcgaaga tgcgctcgcg ccactccgcc
     8041 ggaatgcccg gcccgtcgtc ggtgatggcg agatacgtcc ggccgtcctt gcgccagccg
     8101 tagatatcga tcgtgccctc ggcgggcgcg tacttggccg cgttctcgag cagataaccg
     8161 agcagccgct tgagctgatc gactgacgcg tacgcccgca gcgggcgctc aacaaaggac
     8221 gcggcgagct tctggcggcg gaacatgggc gccatctcgc gtgccatgtc atcgacgacg
     8281 ctggccacgt cgagcgcgac caccggcccg gaatccgggg tcaggacgcg caggctggcc
     8341 aggatggaat ccacggtctg gtcgagccga tcgacctgct ccagcgcgct gcgctcccag
     8401 gcggacgcct cgctcgcttc atcgcggccg gccatgcgct tggtcgcgcc accgagcagc
     8461 tcgacgtagg cacgcacgac cgcgagcggc gtgcgcagcg cgtgggtgac gaccgcgaca
     8521 aggtgcgccc gcgcctcatc gatggcccgc agggactcga gctgcgcctc cgcctcgcgc
     8581 tggagccggt tgcgctcgac tattccagcc agcaagccgg cgagcgcctc gagaaaggcc
     8641 acgtcctggc gggtgaagcg ccgccgccgc tcggtctgga cgttgagcac gcccacgacc
     8701 tggtcgttcc agatcaaggg cacgctgcac atggaggtga acttgtcctg gtccacgccg
     8761 cggatccagt ggaagcgctc gtcggcgtgg acgtcgagcg ctgtctgggg ttcgcgcgtc
     8821 tgggcgacga tgccggtgat gcccacgccg agtggcagcc gcgccagccc gatctgctcg
     8881 ggatccagtc cgttcgtggc ggccagggtc acgcccaggc cgtcgcgatc gagcaggtac
     8941 agcgagcaca cctcggcctg cgccgcagcc gttgcgcggt cgacgatcgt gcccatgagc
     9001 tcgtcccacg tgcgggcaga gccgaccagg cgcgcgacct cggccaggaa ctcgagctcg
     9061 gccaggcgcc gcgcaccccg tttgggcgcg ccccgcgcac gctggattcc agtcgccacg
     9121 ggggtacgat agccagccca agcacacgga ggctgcgaac ccatgacgga cacgctcacg
     9181 cgcggagcgg ccaaccaggc cggggcgcac atcaacaagg acgcttacgc ccaggcgcag
     9241 gacttcctgc cgatcaaggg catcgattac gtcgagttct gggtgggcaa cgcccgccag
     9301 gcggcggcgt actaccgtgc cctgtggggc ttcaccaacg tcgcctacgc cggcctcgag
     9361 accaagatcc gcgaccgcgc gtcgtacgtc atggtccaga acgacatccg cttcgtgttc
     9421 accgcgccgc tcaccccgga cggcgagatc gccgagcacg tgcgcaagca tggcgacggc
     9481 gtgcacgaca tcgccttcgc agtcgacgac gtcaagtcgg cctggacgga gacgaccaag
     9541 cgcggcgcca agtcctacct ggagtacgcc gagtcatcgg atgacgacgg cacgctgcgc
     9601 cggtcagccg tgcacaccta cggcgaggtc atccactcat tcgtcgaccg gtccgactac
     9661 cacggcgcct tcgcgccggg ctaccgcaag gtcaagaagc ctgcgcgcgc ggcgacgggc
     9721 ctgagcctgc tcgaggtcga ccactgcgtc ggcaatgtcg agctgggcga catgaacaag
     9781 tacgtcgatt actaccgcga tacctttggc ttcagccagc tcatccacta cgacgacaag
     9841 gtcatccaca ccgagtactc ggcgctgatg agcaaggtca tgaccaacgg caacgggcgc
     9901 atcaagttcc ccatcaacga gcccgccagc ggcaagaaga agagccagat ccaggagttc
     9961 ctcgactggt atctcgacgc cggcacgcag cacgtcgccc tgcgcacaga ggacatcgtc
    10021 ggcaccgtgc gcgcgttgcg cgagagcggc gtcgagttcc tgggcatgcc gcacgagtac
    10081 tacgccgcac tgcccgatcg agttggcgat gtgggcgtgc cgatcgatgt gctcgaggag
    10141 ctgggcatcg aggccgaccg cgacgaagag ggctacctgc tgcagatttt cacgcggccc
    10201 gtccaggatc ggccgacctt cttcttcgag atcatcgagc gccacggctc acgcggcttt
    10261 ggcgtgggca acttcaaggc gctcttcgag gccatcgaga gggagcaggc attgagaggg
    10321 aacctgtaga tgtcgaatgt ccgctgggca cgcatcggct ttgcgctgct ggcctggcta
    10381 ctggctggac tggcgattgt ccagatctac ctcgccggtc tggccctatt cgtagtcgac
    10441 tcgacgttcg agatgcaccg gaacttcggc tacctgatcg ggatcatcgc cctcgtccag
    10501 ctggtactcg cattcgccgg caagcttggc ggccggatga tcggtaccag cgcactgctg
    10561 ctcgggttga tggccctcca gtcggtcctc gtgatcatgc gcgccgatca gccgacgatc
    10621 gccgccatac atccggtcaa cggcttcctg atcgtgctgt tgtcggtctg gatcgcgtgg
    10681 cgagcgctgg gctacatccg cgcaccgcta cccccggagc ctgtcgaggc cgccccagcg
    10741 cccgctgcca cacattcgcc aaggccgaca ctcgatcagc aggacgaaca agaggatcgg
    10801 ctctaagcgc cacgacgagc agcaatccct ttaccgatac ggactcagcc gcctgccact
    10861 aggcctcaat caggttgtcc ggaaccgggt atttgaccag ggcgtggttg cgggtgatga
    10921 gccgagagac gagatgcgcg cccgttgctc ggtcaatcac ggtctgccat agctcgtttt
    10981 cgcggtagac gcgtccgtct tccgcgcgcc ggaaacgatg gccgcgctct tcgacctcat
    11041 gttgcgtggg cagctcgcga tcactcctga tcgccccgag cagatacgtg tctgtcagct
    11101 ggaccgatat ctgaaatgtc gcgccagtcg tgttgagcaa ccgtagatcg ccgtagttgt
    11161 agaagacgct cgcgcccgat ccaaacggct gcacgcgaac gtcgtcggga aatgggtcga
    11221 agccatggtg atgctgttcg gtcaccgcca gcggggtgtg gagcgccatc cagtacagca
    11281 ggttcgacat ctggcacagc ccaccgccaa cgtcgatccc tacctcgccg tggcgcagaa
    11341 ccaggccggg agcgaaacca cgcctctccg ttgggttgcc aacgcggtcc cagaacgaga
    11401 aaagctcacc cgggtgaacc agcaacccgt cgagtgatct cgccgcgagt gcgagattga
    11461 cgatcttcgt ttcctggtag cgcgggtcca gccccgcgag gcgacggcgt agacgcgact
    11521 catgcttgac cagtgtcacg ggcagcgagg acgacagctg cacccgcgcg aagtgcgggc
    11581 gcctgatgcc gtccgccagc cgccgggcgg ccaccagctg agccgtgcgc agacgcaaca
    11641 gagcgggatg cagctccgaa agccgcgtcg aacgtttcac ccggcccagc ctagcgccgc
    11701 ggtccttcga ctcggcgacg agggccgtta ggcggctgcg gccgcttgct cgatcggctc
    11761 gagaacgacc gcggggatga tccgcgtcgt cttcttctcg tactcccaga agccgggcat
    11821 gatcgtcgcc tgctgggcga acagccggtc acgctcggga ccggatggtt gaggcgtggc
    11881 gttgacgcgg aacttctccg taccaacctc gacttcggcc tccgggttgg ccaggaggtt
    11941 gtggtaccag gcagggtgct tcggcgcgcc gcccatcgag gcgatcacaa cgtagcggcc
    12001 gcgatcgcgg tgaaacgcga gcggcgtcgt gtgcgctttg cccgaacgcg cgccgcgcgt
    12061 cgtcaggagc agcagcccgt cgccaaacgg ccctacgcca cggccgccct ttgcgcgaaa
    12121 ctcatcgatg atcttgttgt tccagtccga catgtcctta gttcccgtct aggttcgcga
    12181 agcgccttgc cttcgctgct atttgcaata caatacagca tcgtatggta attgagaaga
    12241 cccccgcctc gtgccgcggt cggcggcgcg acgacacgat cgacgcgcgc gtgctcggca
    12301 tcaccaatcg ccacctcgct gagcgcggct tcgcggcact gtcgatctcg gccatcgccg
    12361 acgaggcgtg taccacgcgc caggcgctct accgccgctg gccaaccaag gagcggctcg
    12421 tcgccgatgc gatccgaatc gcgggcggta gcgatgcggt ctgcgtatcg gacgatccgc
    12481 gggccgatct ggagcacgag ctggcggcgt gggtccagga cgacaccacg gccggcctgg
    12541 cgatggcggg ggcgatgctc gagcgcgaga cgccggagga tgcgcgcgac tgctatcgcg
    12601 aacacgtcct ggcgccgcgc cagcagcgca tgaccgacat cctggttcac gcccaggcgc
    12661 tgaagaggat tgatgtcgac gccgacgtgg cgggcgcggt cggggtggca attggcgcgg
    12721 cctacgttgc gcacctgtca ggctcgattg cccccgactg gccggcacgg acggccgcgc
    12781 tcgtgtggcg cgcgctgggc ggcaaatgaa gatcgcccac gcccgcgagc ggcacgcgcc
    12841 ggctggcgcg tcgttccggt tgatggccgc gctcgacgag gccggccggc aatggctcga
    12901 ccttgaggtc gcgcgacgcc gcgccgtgaa tgccgacgag cgcctcgccc acaacgaggt
    12961 cctgttccgc cagcccgtga cgacgctcga cgaccacctc gcgcgcggcc tgagggtcgc
    13021 ggccctgggc gcgctgctcg atgtctacga gccgcgcggg gccgacgacg acgtggttct
    13081 cgatggcacc gatctcagct tcgggccgcc catcctgcgc ccggccagcg tgcgcgactt
    13141 ctacgccttc gagaagcacg tgggcaccat ctggtcgcgc cgcggccagg agatcccgga
    13201 ggcgtggttc cgcctgccga tcttctactt ctcgaacgtg tccgaggtgc gcggcccgga
    13261 ccagccggtc tggccgccgc gcggctccag cgagctcgac tacgagatgg agatcgcggc
    13321 gctcatcgac acccctgcgc agaacctcga cgcggcgcgc ggcgaggagg cgatcggcgg
    13381 gttcatgatc atgaacgact ggtctgcccg cgacctgcag cgcgacgaga ccgccgtccg
    13441 gcttggcccc gccaaaggca aggacttcgc caccacattt ggcccgtggc tcgtcacgcc
    13501 cgacgagctg gccgacaagc gatcaggcaa ggcatacgac ctggcctgca cggcggaggt
    13561 caatggcacg atcctgagtc gcagcaacct gggcaccatc catcactcgt tcggggcgat
    13621 gatcgagcgt gcctccgccg acgtgcgcct cgtgcccggt gacgtcatcg gctcgggcac
    13681 cgtgggcggt ggctgcctgc tggagatcaa ggacgaatcc ggtttcggtc ggtacctcca
    13741 gcccggtgat cgcgtgacct tgaaggtcga gcgcctgggc gagctctccg cgcccatcat
    13801 ccagaggccc tagaacgccg tcttgacacg ccgtcacgtc cttgtgctag tatccctact
    13861 aatctgatgg acttactcgc acaagcaggg taagcccggt ccccacgcac ggaggcacac
    13921 gacggtgtat ctgctcctcg gcatcgcgct tgcagggttc ctggcgcagc tgatcgacgg
    13981 ctcgctcggc atgggctacg gcgtctcttc gacgacgctg ctgctcacgg tcgggctcac
    14041 ccctgccctg gcatcggcca gcgtccacct tgccgagatc ggcacgaccg ccgtatcggg
    14101 cgcttcgcac tggcgcctgg gcaacgtcga tatgaagatc atggcgctgc tggccatccc
    14161 cggcggcgtc ggcgcattcc ttggcgcggt cgtgctgtcg aatctctcga ctgaggctgc
    14221 caagccctgg gtgagcgtcg tcctgctggt ccttggtgcg gtcatcgtct tccgctttct
    14281 gcacgcgcgc cgcacccgcc aggcgcccgc cgtgctgcac gaccagccgc tcaacgccgc
    14341 gccacgccgc agccggcgct ggatgctcac tccgctgggc gtcgccggcg gcttcctcga
    14401 cgcgtccggt gggggtggct ggggtcccgt cgtgacctcc accctgatgg cgagcgggcg
    14461 gctcaagccg cgcacgatca tcggcactgt cagcatgtcc gagttcgtcg tcgccatcgc
    14521 ggccagcgtg ggcttcctgc tcgcgctggg cgcggcgggc atccgctggg acatcgtggc
    14581 gatgatgctg gtcggcggct cgatcgccgc gccggttgcc gcgtggctcg tccgcaagtt
    14641 cgacgaccgc gacttgggca cggccgtggg cgcgatcatc atcctcctca acatcgatcg
    14701 cctgctcgtt ctcctgggta tggactcgtc gctggtcaca gccgtccgcc tgttcgcgat
    14761 cgcgtctgcc gcggtgatca tcgggatgct cgtgtggcgc ggtcgcgctg gagccgtggg
    14821 gggcgcgacc gcaagcgcct agaccaccgg gcgcgctacg cttgcgcgcg tgttctacgt
    14881 aagccgcggc aacgtcccgc acacgcgcca cacgcagcat cgcgcgcccg atggctcgct
    14941 ctacgccgag gagctcttcg gcatggaggg cttcaccggc cgatcgtccc tcctttacca
    15001 ccgcgtgcca cccacgcaga cgcaccagat cgagcgcgtc agcgaggtgc acaaggaggc
    15061 aatcgacgat ggcgttcatc gccatcggct ggtgaagacc ggcggactgc cggcggctgg
    15121 caacgttgtc gaggggcgga tgccgctgtt tttcaacagc gacgtcctga tgggtatcgt
    15181 ccggccaacg gagaacctgg cagccaacat cttctaccgc aacggcgagg ccgacgagat
    15241 gctcttcgtc cacactggcg agggcgtctt cgagagtaac ttcgggccga tgcgctatgg
    15301 ccccggcgac tacatcgtga ttcccatcgg caccacctgg cggctcaacc aggacgccaa
    15361 gtcggaccac cgcatcctgt atgtcgagtc gccctcggag atcgtgccgc ccaagcgcta
    15421 ccgcaacgac tggggtcagc tgctcgagca cgcgccgtat tcacaacgcg acatacgcgt
    15481 acccgacgat gttgccgtgc acgacgaggc aggcgacttc gtcatctggg tcaaggtccg
    15541 cggccggatc accgcctacc actaccgcca ccatccgttc gacgtggtgg gctgggacgg
    15601 ctacctgttc ccgttcatct tcaacatcgg agatttccag cccatcaccg ggcgggtcca
    15661 ccagccgccg cccgtccacc agacgtttgc cggccgcaac tacgtcgtgt gctcgttcgt
    15721 gccgcgcaag ttcgactacc acccgctcgc catacccgcg ccctacaacc actcgaacat
    15781 caactccgac gaggtcatct actacgtcgc gggcaatttc atgagccgcc gcggcgtgga
    15841 catctcgtcg ttcacgctcc acccggcggg catcccgcac gggccgcatc cgggcaccgt
    15901 cgaggcgtcg atcggcaagg aagcgaccga ggagctggcg gtgatgatcg acacgttcca
    15961 cccgctgaac atgacgcgcg aggccgcccg cctggaagat gcgtcgtacg cgtattcctg
    16021 gctgccgccc gaggacggcg gcgagcacgc ccgcgagttg tctgagcgcg gcccggaggc
    16081 atttcccgac tgattccggg atttctgaac gtttggcgat acgggatgcc tcatagtgtt
    16141 tagaccgcgc ctcccggctc ggcacgttag aggtccatat ggacccagcc gtcgtcgagc
    16201 gcgccagggg cggtgaccgc gaagcgttca ccactctcgt aggtcaagtc agcgattcgt
    16261 tgtacgcggt cgctttccga atcctgcggg agaccgcgtc tgccgaggat gcgctccaga
    16321 acgcccttat ctccacgtgg cagcagctgc cgcacctgcg cgatgtcgag cgcttcgagg
    16381 cctggtcgca gcgcatcctg gtccacgcct gctacgccga attctctcgg cgccgtcagt
    16441 tcgcggccaa cgttcgtgtc atcgatttga accgcgcggg cgcgtctgac ggcagccgcg
    16501 tgctggccga tcgcgacgag ctcgaccggg cgtttcgcaa gataccggtt gaccaacggg
    16561 cggtcttcgt catgcatcac tacgtcgggc tgcccctggt cgagatcgcc gagacgctcg
    16621 gcatccccgc ggggacggct cgctcgcgac tccacaacgc cacgcgggcg ctgagaacgg
    16681 tcatggcggc cggcgctctg cgaacgctcg aggaaggacg accggcatga gcgacgaccg
    16741 gatgttcgag cgcagcgctc aggcttggct cgaattgggc ccgtcgaccg cacccaagcg
    16801 ggtcgtcgac gcggcgctgc gcaccatcgc aacgacacca caacagcggg acctccaggc
    16861 ctcgtggagg ttccctcgct tgacaatcca aatgcgtttc gcagtcgccg ctgccgttgg
    16921 cctcatggtc cttggcgccg gcctgctcgt cctgcgcagt ccgcagaacc agatcggtca
    16981 aaccccgacg ccatcaccct cggcgagccc gatcacgatg gagcggctgc agggcgcgtg
    17041 ggcttccgtc ggcacgcgca tcgacccaat tctggggaac gctccagaca aatcaggtct
    17101 ccaaacgatt gcggtggacg cggcaagcct ccgcctcaac gggtggaagg gagaaatcgc
    17161 cagctctgct tcggtcatcg gtgccgcagg ccagctcgaa ttgctgatgg acgcgccgca
    17221 ggagtcgttg aaccgagagc actgggactg cactaccggc gatagcggca cgtactacgc
    17281 cagcctatcg ccggatgacc agacattgac cctgacgtca gcgagcgacg agtgcgcttc
    17341 gcgcgacgag atccttactg gcgattggac ccgctggtcg tgtccggatc ctgctcggta
    17401 cgcctactgc agccacgtca gcgaacttgc ccctgggcga tacacggctg cgaacttcaa
    17461 accgttcgaa gggggcaccc gcggccagtt cgcgtacacg gtgcctgccg gttgggcgga
    17521 ctttcacttt ccgccaacct ggcctggtcc cgacaggcga gcggacctta ctttggcaac
    17581 tgtcgcgaac cctgactctc ccttgatcaa cgtcgactca actatccggg cgagcacgat
    17641 gtccgcagta atcggtgcgc ccccgctcgc ctgccctgac cccgtcaccg cttcgggagg
    17701 gccggccgcg agtgtcgcgg actggctcgg gtcgcgtccc ttcttggccg tttcgcagcg
    17761 gagaccgatc acgattggcg gactcagcgg tgtgcagatc gacgtctccg tggtcgccgg
    17821 gttcgtggat ccatgtacgc tcgggtcagc gccaggagca ccaatcgagt attcgacgca
    17881 gatcgagatt ttcggtggct cggagaccgg cgattcggtc accatggaca ccgctacgga
    17941 cgatcatgcc cgcttcatcg tgctagatac gggtgactgg caaggctacc caggaaccct
    18001 gctgatctca cttaccgcgc aaggccaatc ggcgctcgat gttgtcatcg cccaggctat
    18061 gccggtcgta cagaccttcg agttcatccc aggcccaagt gccagcccga ccgcggtcag
    18121 ctcgccgatt gcggttaacg caactgacct ccaggggacg tggaactccg tggggacacg
    18181 cccaatcccc gacgctctcg accaactgac tgtgttcacg cttggcgcca attacctcga
    18241 catccaccaa tcggacgggg acgtcagaag ctcgtgggtg ttaatagagg acacaatcaa
    18301 tcgggtttgg gtgcatggtc cgcttaccac tgtcccggtc gctccgcgtt gggattgctt
    18361 cggcatcggc gaatacgatg tcagcctttc gaccgacggg gcgacgctca ccttcactcc
    18421 catagacgag gactgcgccc aacgagaggc catcctggcc ggcgattgga cgcgggacgt
    18481 cgcggccgag ccttcgctga agtgaatgca tcggccgtag ccatcgtctg cacccttctg
    18541 atcgtcggcg cgtttaactc gggctgcgcc ggttcgccgc ccgcggtcac ccctactcca
    18601 gctccatcag cagcgccgac ggcggcgccg acggccgggc ggacggtcgt tccgacagcc
    18661 gtgccgacag ccaaaccgac tgcctcgccc tctccggtgc cgactccggg gttgttccgg
    18721 gcaccggtgg tcctggacca ggcgatgttg ccgatcgtcg tcgctgactt cgatggcgat
    18781 ggcaacctcg acatcgcggg cggcgcggcg gacgcacgtg tccaactgct cctgggagtg
    18841 ggcgacggca gattccagcc gggtgcggag atgccggggg gcaacgggcc cgcctcgatt
    18901 ggcgcggcag atttcaacgg tgacggccgc ctggacatcg tgatggccta cagcggcgtc
    18961 agcgaccaag gaactggcga cgacgacatc ctggtgttct tggcgaagga cgatgggagc
    19021 ttcgaagcgc tcgtccaacc cgcgggagtc aacccgcaag cgctcgtagt cggagacttc
    19081 aacgaagacg aggtgcccga cctggcaacc gccaacggct ttgattacct ctcgatcttc
    19141 gccggtctcg gcgacggcac attcgcagat ccggagaact acccgatcaa tgccaggttc
    19201 gcgtcgggca tcgcggccgc cgatttcgac ggggatggca acctcgacct ggtcacgacc
    19261 aactcgatga tcggcaaggg tcggagcgac cggaccatgt cggtgctgct cggcgcaggc
    19321 gacggcacgt tcggggagcc gcaaacattc cccgtaagcg gtccacagcc aatcatgccc
    19381 gtagtgggcg acttcaacgg cgatgggttc gccgatgttg ccagccctga cgggtaccca
    19441 accagggtca cgagcgtgtt gctgggcctc gggaatggtg ccctcagaga gacctcgtac
    19501 tacctgtgtg gcccaaatcc gcacaccctt gctacggcag acctggacgg agatgggatc
    19561 ctcgacctcg tctgcggcaa cgccggcgaa gagggcggcc ctgtcggcaa ggggatcgcg
    19621 gccctattcg ggctcgggga cgggacattc aagccgggtg tcgatctcgt cggcaaccaa
    19681 ttcggtgagg ggatagcggc cgcggcagat ctcgataacg atgggcagat cgatctcatc
    19741 gtgtacagcg acaccctcgt ggtgctgttc aacgacattc ctcgctaacc ccagccgctg
    19801 cgttatggtc ctcaccgtgg atgccaacga tctcgtacag atgctcgaag acgtcgaagg
    19861 gcgtctgatc gaagacgctg cggggccggc gccgagtggg ctcacccaac ccgacgaagg
    19921 tggcaccgag cgctgggagg cggcccaggt ctgggcgcac atggctgagt tcgtcggcta
    19981 ctggcaggcg caacttgagc acgtcgtcga cagctacgcc ggccagcccg tgccgtttgg
    20041 tcgcaccaag gaggatccgg gtcggatcga aggcatcgag accgggcgca gggtcccgat
    20101 agcgcagctg atgcagcagg tccacgacgg catcgaggtg acgcgccgct acctgccgac
    20161 gctcagcgcc gcacagtggg cgtcggtcgg tctgcattcg cggcgaggcg agatgaccgt
    20221 gccccagatg gtcatgcgat tcacaatcga ccacttagag gagcacgccg accagctcga
    20281 cgacctggcg tgaaggtcta tctcgagact ggcgagaaga aatcgttcgc gaccgcgatt
    20341 gactggatag gcctggcccg gggcggcaag acgcccgacg acgccatggc ggcgctcgtc
    20401 gaatacacat cgcgctacct ggagtcgatg ggcgcggccg ccgccgcgct gaaggccccg
    20461 gccacggttg ccgggctgaa cattgttgag cgcctgccgg gcaacaagac gaccgacttt
    20521 ggggcgccgg atgccatcct gccggccgac cggaagccgc tcagccaacg cgacctggat
    20581 gccgcgatcg aaagccttca cgcggcgtgg gccgccttcg caacagcggc aaagcgcgcc
    20641 ggtggcaagg atctcgcgcc atcgggcccg cgcggtggcg gccgatcgct cgacaagatg
    20701 actgcccatg tgcacgaggc gcagggcggc tacacatacg cggtcggcgg gaagtcgaag
    20761 gctggggcgt catggtcgga gatccagcgc gacttcatcg gtgcgctgcg cttgcgtaac
    20821 gccggcgcgc taccggactt cgggccgcgc ggcggtgagc gctggccggc cctgttcgcg
    20881 atccgccggt cggcgtggca cgcgctcgac cacgcctggg agatcgagga ccggctgggc
    20941 taggcgccga cccgcaacac cgactcggcc aggatgtggc cgaccagcag aacagctatg
    21001 aacagcgcca gcagcccgat caacacgttg gctgccttca gtcggacacc caccacggcc
    21061 tcacggccgg tagcttccct atgcagctca actctgacca gtggcgcggc cgcgatcagc
    21121 aacgcgatga ccggcgacac aacgagcatc aagtcgatga tgcgcacggt ctgcatccag
    21181 cgcgtgaccg gttccatgaa tgtgatcagc catgtcgcgc cgagctggta ggcgaggatc
    21241 gacagcagaa caaatgccgc cgtcggggcg aggatgatga gcgccatggc cgcccacatc
    21301 tgcggatgac gccatgccgg gccgatgacc acgcttccac ctcctgccaa tccgagccgc
    21361 gcctggttcg tccattcgcg cgcagcgccg cgcgccacgt cgatcgcgac gcgcggggtc
    21421 atgccggttt cgtcaatcag atcggatagc tccgcgccgt agcggtcgcg ccaagcgcgc
    21481 ggatacagcc gtgtgatcaa gcgcgtcatg cactcacccg tgctaggccg gtcgcgaccg
    21541 cgcgctggag gctgccgaga cgctgctcca gtgcggcctt gccggcaccg gtgagtcggt
    21601 agggtcggcg cctgccctcg gccgggaggg gctcgacgtg gccgctcgcc tcgagccggg
    21661 agagggcgcc gtatagcgtt ccggggccca aacgaacgcc cgcgaagcgc tcaacgtcga
    21721 gcagcatcgc gtggccgtgc ttggggccgg cggcgaggct gatcatcagc atcaggcccg
    21781 gatcgatggt tgcctggata tcggccacta ctacgtgctc cgtactacgt ggcgcgtagt
    21841 attagcgccc gggcgccgcg tgtcaagcct tcttgccgcg gccctcctgg aggacgctga
    21901 gcaggatcga gatcacgttc tgcggccgct cgcccaggcg gcgcatgtag tacgggtacc
    21961 actggctgcc gtacggcacg taaatgcgca cggtctggcc cagcgccacg aggcgctcat
    22021 gcaggtcacg ccggacgccg tagagcatct ggaactcgta gcgcgccggg tcgatggcgt
    22081 gctccttgac gaacgcgagc gcgtgatcga tgagccgatc gtcgtgcgtc gccagggccg
    22141 ggtagttgcc gtccgtgagc agccgctcca tcagctcgat atacgagcgg tcgacctctt
    22201 cgcg
//
LOCUS       DATBRX010000123         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1492371, whole genome shotgun sequence.
ACCESSION   DATBRX010000123 DATBRX010000000
VERSION     DATBRX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492371"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VIF08_04410"
     CDS             <1..1101
                     /locus_tag="VIF08_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY8135261.1"
                     /translation="RVMVHPDVADTVDSMLRGDPIAPELRWRDEGGVHRSQARPRPGP
                     QSQIPGERFGGLVGIGGGGFRGEPGWRPDSRPFEQERGYRPGYRPGASGGWRRGPGGS
                     NGGAGGGGPFRQGDLADRGPLERGTPQADHRARDAREFERQQAWRDSAAKAITQLRAD
                     ETRGPAVLDEDDDEDIELDALEQEAQFDEEELTGDRRPTPLPAVGDTSLPTLHVLGYG
                     VSHKRLEQAVRELGLPVTLVRDAEQADVVITLRNYFKQKAPALREAEERGLPIFVLKS
                     NTMVQMESALTSVFALEIDPRDAALREVEEAIGLVNEQAKPVELSPQNAYIRRLQHQM
                     AERANLVSHSRGREPYRRVRLYPDNGAARIRR"
     gene            complement(1114..2439)
                     /locus_tag="VIF08_04415"
     CDS             complement(1114..2439)
                     /locus_tag="VIF08_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HEY8135262.1"
                     /translation="MAICAAAALAALVASTSADAAAYPLLLKDINPTGSSNPSQMTAV
                     GSTLFFAANDGVHGNELWKSDGTAAGTKMVKNIRPLGKSSDPQNLVNVNGTLFFTAND
                     GAHGRELWRSNGTAAGTKLVKDLIPQCSGGFGCIGGLLIEIPPVAIGSRLFFINDYCC
                     YGTSTIYVSDGTAAGTKQLKTNLPVSTTVGAATGYNGKFYFAAYTTYGEYASVWVSNG
                     TRAGTYDLGAASGDDIAFLPVSGQSLYFATYYEDETEIDVQLWKTDGTAAGTKPLTSV
                     NALAEMPIEAALMNSRLYFNDGPLGSLWKTDGTAKGTKSISAGRAEWLTVASGKLYFT
                     RGGHLWMSNGTPTGTADVEGFGGLYARQLMAVGAEICFGVPDWDAATWTLWESDGTDS
                     GTYAVRSFVNPIDDGWGPLGAKLGATLGATLVFGADDGTHGTELWSYAP"
     gene            complement(2584..>3258)
                     /locus_tag="VIF08_04420"
     CDS             complement(2584..>3258)
                     /locus_tag="VIF08_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135263.1"
                     /translation="HGKTVMAAYLVGSRGTTRQAIGLGLTVTVSHTLGVLALGLLSLS
                     AAAVIPVERLYPVLGVTSGAIVVVIGGYLLITRIRAWRADSKPHHHGHHRSIVDRPDG
                     WHEHDGMGHTHLPSQGMGKRGLFALGLSGGMIPSVSALLVLIGSISIGRPAWGIVLTV
                     AFGLGMAAVLVGVGLALVHARKLVERLPVGRALRFGQRLPVVTAAVVLVAGLLIAGQG
                     LLAITG"
BASE COUNT          541 a         1072 c         1119 g          526 t
ORIGIN      
        1 cgggtcatgg tccatcccga cgtggccgac acggtcgatt cgatgctgcg cggtgatccg
       61 atcgcgcccg agctgcgctg gcgcgacgag ggcggcgtgc atcgatctca ggcccggccg
      121 cgtcccggcc cgcaatctca gatacccggc gagcgctttg gcggtctggt tgggattggc
      181 ggcggcggct tccgcggcga gcccggctgg cgtcctgact cgcggccgtt cgaacaggag
      241 cgcggctatc gacccggcta tcgccccggt gcgagcggcg gctggcgccg cggcccgggt
      301 ggctcaaacg gaggcgccgg tggcggcggc ccctttcggc agggagacct agccgaccgc
      361 ggaccgctgg agcgcggcac tccgcaagct gatcaccggg cacgcgacgc gcgcgagttc
      421 gaacgccagc aggcgtggcg cgattcggct gcgaaggcga tcacccagct gcgcgcggac
      481 gagacccgcg gcccggccgt gctggacgaa gatgacgatg aggacatcga gcttgacgct
      541 ctcgaacagg aggcgcagtt cgacgaagag gagctgaccg gcgaccgccg gccgacgccg
      601 cttcccgccg tgggcgatac gagcctgccc accctgcatg tcctgggcta cggcgtgagc
      661 cataagcggc tcgagcaggc ggtgcgcgaa ctcggcctgc cggtgacgct ggtgcgcgac
      721 gcggagcagg ccgacgtggt gatcacgttg cgcaactact tcaagcagaa ggcgcccgcg
      781 ctgcgcgagg cggaggagcg cggcttgccg atcttcgtgc tcaagtcgaa cacgatggtc
      841 cagatggaat cggcgctgac atcggtcttc gcgctggaga tcgacccgcg tgatgccgcg
      901 ctgcgcgagg tggaggaggc gatcggcctg gtcaacgagc aggccaagcc ggtcgagctg
      961 tcaccccaga acgcctacat ccgcaggttg cagcaccaga tggccgagcg cgccaatctg
     1021 gtctcgcact cgcgcggccg cgaaccctat cggcgggtgc gcctctatcc ggacaatggg
     1081 gcggcgcgca ttcgccgctg agaggtcggc cggctagggc gcgtagctcc agagctcggt
     1141 gccatgggtg ccgtcgtcgg cgccgaagac cagggtggct ccgagtgtgg ctcccagctt
     1201 cgcgcccagc gggccccaac catcgtcgat cggattgacg aacgaacgca ctgcgtacgt
     1261 cccgctgtcg gtgccatcgc tttcccacag agtccacgtc gctgcgtccc agtccgggac
     1321 gccgaaacag atctctgcac cgaccgccat cagctggcgc gcgtacaggc cgccgaagcc
     1381 ctctacgtct gcggtacctg ttggtgtgcc gttgctcatc cacaggtgcc cgccgcgggt
     1441 gaagtacagc ttgccgctgg cgacggtcag ccattcagcg cgtccggcgg agatggattt
     1501 ggtgcccttg gcggtgccgt ccgtcttcca tagcgatccc aggggcccgt cgttgaagta
     1561 gagacggctg ttcattagcg cagcctcgat gggcatctcg gccagggcgt tgaccgacgt
     1621 cagcggtttg gtccccgcag ccgtcccatc tgtcttccag agctggacgt ctatttcagt
     1681 ctcgtcctcg taataggtgg cgaagtacag gctctggccg gacacaggga ggaaggcgat
     1741 gtcgtcgccc gaggcagctc cgagatcgta ggtgcctgcg cgagttccat tgctgaccca
     1801 cacgctggcg tattccccgt acgtggtgta agcggcgaaa tagaacttgc cgttgtatcc
     1861 agtcgctgct ccaaccgtcg tggacacggg gagattggtc ttcagctgtt tggtgccggc
     1921 agccgtccca tcgctcacgt agatcgtgga ggtgccatag cagcagtaat cgttgatgaa
     1981 aaacaatcgt gagccgatgg cgaccggtgg aatctcgatc agcagaccgc cgatgcagcc
     2041 gaagccgccg gagcactgcg gaatgaggtc cttgaccagc ttggtcccgg ccgccgtgcc
     2101 gttgctcctc catagctcgc ggccgtgagc cccgtcgttg gcggtgaaga agagcgtgcc
     2161 gttgacgttg accaggttct gcgggtcgga gctcttgccg agtggtcgga tgttcttgac
     2221 catcttggtg ccggcggcgg tgccgtcgct cttccacagc tcgttgccgt gaacgccgtc
     2281 gttggcggcg aagaagagcg tgctgccgac cgcggtcatc tggctgggat tagatgagcc
     2341 agtcgggttg atgtccttga gcagcagcgg gtatgcggcc gcgtcagccg atgttgatgc
     2401 aacgagcgcc gcgagggccg ctgccgcgca aatcgccacc aaccgtcgca tgccacgctt
     2461 ctttcggcga ttggttcccg gcgccggatg gcgcgcccgt tagagccgat ttcgccgagt
     2521 acaggctccg tcgagcgcga gtcgaccaac aggaccggcc ggcccgtttc tgagggccgg
     2581 acgtcacccg gtgatcgcga gcagcccctg accggcgatc agcaggccag cgaccagcac
     2641 caccgcggct gttaccactg gcaggcgctg gccaaagcgc agggcgcggc ccactggcag
     2701 ccgctcgacg agcttgcggg catggacgag agcgagaccc accccgacca acacggctgc
     2761 catgcccagg ccgaaggcga ccgtcagcac gatgccccag gccggccgac caatcgagat
     2821 cgagccgatc agcaccagca gggccgatac cgacgggatc atcccgcccg acagacccag
     2881 ggcgaacagc ccgcgcttgc ccatgccctg gctcggcaga tgggtatggc ccatgccgtc
     2941 gtgctcgtgc cagccatccg gccgatccac gatcgagcgg tgatgcccgt gatggtgcgg
     3001 cttcgagtcg gcacgccaag cacgtattcg agtgatcagc agatagccgc cgattaccac
     3061 gacgatcgcg ccggaggtga cgcccaagac ggggtaaagg cgttcgacag ggattaccgc
     3121 ggccgcggac aggctgagca ggcccagcgc cagcacaccc agcgtgtgcg acacggtgac
     3181 cgtcagcccc aggccgatcg cttggcgcgt agtgccgcgc gacccgacga ggtacgccgc
     3241 catgacggtc ttgccgtg
//
LOCUS       DATBRX010000124         8211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2273777, whole genome shotgun sequence.
ACCESSION   DATBRX010000124 DATBRX010000000
VERSION     DATBRX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273777"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1115)
                     /locus_tag="VIF08_04425"
     CDS             complement(195..1115)
                     /locus_tag="VIF08_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8135264.1"
                     /translation="MTAISAPARPVARERAIESGPRPEDHLLRLADGRRLGWSESGDP
                     GGKPVLFFHGFGTTRVVCPPGDAALERHIRLIAVDRPGIGLSDPQPGRSLLDWPRDVA
                     ELADHLGIARFSIVAWSGGGPYGLACGHALASRVDAVAAVSPAAPLAKPTDPGYLRWR
                     DRHAVRFANRAPWVIRLAMWHWGRGQRSDAEKFFEKSVADMCAADQAVLAEPELRSRM
                     IDNSAELYRQGGRGMYDEALVLTKPWGFDPADITVPVHIWQGGRDDTVPAAMATHLAE
                     TIPGARLSMFADEGHHLLYVYWPQILADLA"
     gene            1180..1926
                     /locus_tag="VIF08_04430"
     CDS             1180..1926
                     /locus_tag="VIF08_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY8135265.1"
                     /translation="MFSGWTDRRAVAPIVAELAGTFLFFFVGIGAVASLDRLGGLSSL
                     ASGGMDPAAGLLVVALAHGIVLAVLVSALGAVSGGHFNPAVTLGVWLTGKIHWHRALA
                     YVVAQLIGAILAAWAVRIVIPATLSPSLGVPALGTDISAVAGILVEAVLTVILVIAVL
                     GTAVDLRGPKIGGLAIGFAVAADILMGGPLTGAAMNPARWFGPAAVTGLWDNWYVWIV
                     GPLLGGAIAGLAYRYLFVAEADPAEPATFA"
     gene            2027..3103
                     /locus_tag="VIF08_04435"
     CDS             2027..3103
                     /locus_tag="VIF08_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEY8135266.1"
                     /translation="MGQNLLRKLEDFRLLPLFVIVSMAIGIFIGSVLSISDFALTPPI
                     EAIKSIVGGTFELSVPNAISLGVPLGLFLMMYPAMTNVHLGEVGSAFRSPRQLAVVLV
                     FNYVIAPFLMFALANTFVADPELRTGLILYGLAPCIAMVIVFTYLAKGNTPMALVFVA
                     LNSIVQMLLIPVYARLLIGDVSFDVLVVGESVVLYLGLPLLLGMLTRMLVVRHGGEAA
                     MDRLKPGLNALSILGLLFTLIVMFSLKGDLILERPGIVLEMAIPMALFFGVMFFTVLF
                     VGAGMRFSYRDGVAVAFNATGRDFEIAIAIAITAFSPTVALATVVGPLIEVPVMLFLV
                     WAAMRLAPRLFHSVVLADAPVAAR"
     gene            3100..3429
                     /locus_tag="VIF08_04440"
     CDS             3100..3429
                     /locus_tag="VIF08_04440"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8135267.1"
                     /translation="MRTELPLVPELRLALPAPDTRDAVRVGALVGDPVRAGILAILRD
                     GPHCVCEMAAALGERQNNVSNHLARLREAGLVRASRHGADARWIYYERDEAAVAAALE
                     MLTSRIG"
     gene            complement(3449..4006)
                     /locus_tag="VIF08_04445"
     CDS             complement(3449..4006)
                     /locus_tag="VIF08_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEY8135268.1"
                     /translation="MAMLRFLGLGRQAAASPGAGETRVESETQTVRRIVAELGQLDPE
                     RRRILAGFAYVVSRAAHADLNVTPDETRLMEQIVAEIGGLPEAQAVLVVEIAKNQGEM
                     YGGTEDYLVTREFAAHSTSEQRRAAVEACYAVAAADGAITSEEAAELGQIANELDLPI
                     QDLNAIRRSYADKLLAIQLMRGQRG"
     gene            4065..4796
                     /locus_tag="VIF08_04450"
     CDS             4065..4796
                     /locus_tag="VIF08_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135269.1"
                     /translation="MTRKTSSLALALLATLLVACGGPGASPTPPPTPTPSPTPSPAPV
                     SEVTTAAQAAALVFAHEHIAQIGPLMPDVIGQSSWYEANEEAGGYAVKITVGAGDCQA
                     GCIERHTWTYHVDLDGTVTLIGDEGDDIGLPTDEGTADPLSLRITLSAGPVCPVEQNP
                     PDPACAPRPVANVEVLVFDPSGQQVGEGVSDDHGLINMQLPAGAYYVVAAPVEGLMRN
                     AEPLAFAGVGGDSVALVMGYDTGIR"
     gene            complement(4813..6144)
                     /locus_tag="VIF08_04455"
     CDS             complement(4813..6144)
                     /locus_tag="VIF08_04455"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135270.1"
                     /translation="MNRPRRPFLAALAASAVVLALAGGTAPGALAAAGPNGLIAYSSW
                     NDLLEYDIYVVDPAQPSLPPVRLTTDGAYNDNPDWSPDGSKIVFDGWGELGGPRIQVM
                     DTDPTTDDQQLLSDSQNGYDVQPAWSPNGTRIAFASSRTVGVDEDFGYEIYVMDALGE
                     VGPLANATPLTSDAFDPETGGSIEDSQVTWSPDGSRLAFVSTGRGLDPDTCDLWVMDS
                     ADSDGDGFGDNMHQLTFDESFNCDMFEDVTPQWSPNSSLIAFTSTRNGNFDIWLVNAN
                     DPTDLRNVTATADQNEDQPSWSPDGTQVIFRSGVSGHYELYSLPVPPRAALGSAGQPV
                     AAAAALPTQLTSDGLTKQHGDWGAKAGALRGTAALSVSKVGRGRVSATKIDCGKDCAS
                     TFVVGRSVRLTATPAPGYRFKSWAGACAGTQRTCTVRMGSAKTVTATFVRR"
     gene            6328..7203
                     /gene="thyX"
                     /locus_tag="VIF08_04460"
     CDS             6328..7203
                     /gene="thyX"
                     /locus_tag="VIF08_04460"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY8135271.1"
                     /translation="MTAEAVNPIVATPRADASATPRSPATRDLGMRATLLFPTREIAQ
                     QYYLPLIYTACRTCYSELEPADIFDRATRGQVATEKQQDLIRRVIESGHGSTIEHIVF
                     TFGISGVTRTLSHQLVRHRAGVAFDQQSQRYLNYKKPSYMVPGSLQDAPPEMRDQFTS
                     QMDESLAFYGEMLEAGIPGEDARFVMPNATRTNLIMTANLRSLIHMSGLRLCTMAQWE
                     IRRLFQLIRHEVFSVSPFLGSFLAPKCVPLGYCDEDRNRDEHCPIRPHKDNVLAAWAE
                     KKAAAKAAGRELPTL"
     gene            complement(7251..8087)
                     /locus_tag="VIF08_04465"
     CDS             complement(7251..8087)
                     /locus_tag="VIF08_04465"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY8135272.1"
                     /translation="MSTPSFSQRLDLLEPAVRARTDLVPRVGIVLGSGLGGLADALTD
                     AVAVPFAEFPGWPAASAPGHAGRLLLGRLDGVPVVCLQGRLHLYEGNSERLVVEPVLL
                     MRRLGAQVIVLSNAAGGIRVDWPAGTLMLITDHLNLTGRSPLIGANDDALGSRFPDLV
                     NAWSPRLNELMRQAAADEDVTLEEGVYAGLIGPTYETPAEVRMLRTLGADAVGMSTVL
                     EAVVARWAGAEVVGVSLVTNPGAGITGNPLSHEEVLEAAAEAGSRFQALIRSFVRRLA
                     AA"
BASE COUNT         1283 a         2847 c         2721 g         1360 t
ORIGIN      
        1 cgaccacccg ccccaaccgc ccaagcccgg tcggatctgg cacttgggca agatgctgtt
       61 caacaaggcc tactggcgca cggtcccgcg cggccgggtt tgacctgatc ggtgccgctt
      121 cgagcggcgc caaccgaata agcatgtccc tcccgacggc ggtcgtcccg cagcacggcc
      181 gccgtcggcc ttgtctaggc caggtcggcg aggatctgcg gccagtacac gtacagcagg
      241 tggtggccct cgtcggcgaa catgctgagg cgcgcgccgg ggatcgtctc ggccaggtgc
      301 gtcgccatgg ccgccggcac ggtgtcgtcg cggccgccct gccagatgtg taccggcacc
      361 gtgatatcgg cgggatcgaa gccccacggc ttggtcagga cgagcgcctc gtcgtacatg
      421 ccgcggccgc cctgccggta gagctcggcc gagttgtcga tcatgcggct gcgcagctcg
      481 ggctcggcaa gaaccgcctg gtcggcggca cacatgtccg cgaccgactt ttcgaagaac
      541 ttctccgcgt cactgcgctg gccccggccc cagtgccaca tggccagtcg aatgacccac
      601 ggcgcgcggt tggcgaagcg cacggcgtgg cgatcgcgcc agcgcaggta gcccgggtcg
      661 gtcggcttgg ccagcggcgc ggccggactc accgccgcga cggcgtcgac gcgtgacgcc
      721 agggcatggc cgcaggcgag tccgtacggc ccgccgcccg accacgcgac gatcgagaag
      781 cgcgcgattc ccaggtgatc agccagctcg gcgacgtcac gcggccaatc cagcagggag
      841 cgccccggct ggggatcgga caggccgatg cccggccggt cgactgcgat cagtcggatg
      901 tggcgctcga gcgcggcgtc acctggcggg cagacgacgc gtgtcgtacc gaagccgtgg
      961 aagaacaaga ccggcttgcc acccggatcg cccgactcgg accagccgag gcggcggccg
     1021 tcggcgaggc gcagcaggtg atcttcgggc cgcggacccg actcgatcgc gcgttcgcga
     1081 gcgaccggcc gcgcgggtgc ggatatcgcg gtcatgaccc tcccaggtga ctttggcgtg
     1141 tctagtatgg caagcgatca cattcgagga gggctaagca tgttcagcgg ctggacggat
     1201 cgtcgcgcgg tggcccccat cgtggccgag ctcgcgggca cgttcctgtt cttcttcgtc
     1261 ggcattggcg cggtggcgtc cctcgaccgc ctgggcggcc tgtccagcct ggccagcggc
     1321 ggcatggacc ccgcagcggg gctgctcgtc gtggcgctgg cgcatggcat cgtgctggcg
     1381 gtcctcgtca gcgccctggg cgcggtcagc ggcggtcact tcaacccggc ggtcacgttg
     1441 ggtgtctggc tgaccggaaa gatccactgg caccgcgccc tggcgtacgt agtggcgcag
     1501 cttatcgggg cgatccttgc cgcatgggcc gtccgtattg tgatcccggc tacgctgtcg
     1561 ccgtcactcg gcgtgcccgc actgggcacc gacatcagcg cagtcgcagg catcctcgtc
     1621 gaagccgtcc tgaccgtcat cctggttatc gctgtgctgg gcacagcggt cgacctgcgg
     1681 gggccaaaga tcggcggcct tgcaatcggc ttcgcggttg ccgccgacat cctgatgggc
     1741 ggcccgctca caggtgcggc gatgaacccc gcgcgctggt ttggcccggc agcggtcacc
     1801 ggcctgtggg acaactggta cgtctggatc gtcggcccgc tgctcggcgg ggcaatcgcc
     1861 ggcctggcct accgctacct gttcgtcgca gaggccgacc cggccgaacc tgccacgttt
     1921 gcctgatcgg gccaagcggc cctcgagtag ttgacctgcg gcccgtcagc atcgcatgag
     1981 caaataggac actgttgact gacatatcaa gcagcggtga ggactagtgg gccagaatct
     2041 gttgaggaag ctcgaggact tccgcttgct gccgctattc gtgatcgtca gcatggccat
     2101 cggcatcttc attggcagcg tgctgtcaat ctccgacttc gccctgacgc cgccgatcga
     2161 agcgatcaag tccatcgttg gcggaacgtt cgagttgtca gtgccgaacg cgatcagcct
     2221 gggtgtcccg ctcgggctgt tcctgatgat gtatccggcg atgaccaacg tccacttggg
     2281 cgaggtcggc agcgcattcc gctcgccgcg ccagctggcg gtggtactgg tcttcaacta
     2341 cgtgatcgcg ccgttcctga tgtttgccct cgccaacacc ttcgtcgccg acccggagct
     2401 acgtactgga ctgatcctgt acgggctggc gccctgcatt gcgatggtca tcgtgtttac
     2461 gtatctggcc aagggcaaca cgccaatggc actcgtgttc gtcgccctca actcgatcgt
     2521 ccagatgctg ctcataccgg tctacgcccg gctgctgatc ggcgatgtga gcttcgacgt
     2581 gctcgtcgtg ggcgagagtg tcgtgctcta cctcggactg ccgctgctgc tcgggatgtt
     2641 gactcggatg ctcgtcgtcc ggcacggagg cgaagcggcg atggaccgac tcaagcccgg
     2701 actcaatgct ctctcgatac tgggcctgct gttcacgctc atcgtcatgt tctcgctcaa
     2761 gggcgacctc atcctcgagc gccccggcat cgtgctcgag atggccatcc ccatggcgct
     2821 gttctttggg gtcatgttct tcaccgtcct cttcgtcggc gcgggtatgc gcttctccta
     2881 cagggacggc gtggcggtcg ccttcaacgc caccgggcgc gacttcgaga tcgccattgc
     2941 gatcgccatc accgcgttca gcccgaccgt tgccctcgcg accgtcgtcg ggccgctgat
     3001 cgaggtcccg gtgatgctgt tcctcgtctg ggcggccatg cgccttgctc cgcgcttgtt
     3061 ccactccgtg gtgctcgccg acgcaccagt ggcggcgaga tgagaaccga gctgccgctc
     3121 gttcccgagc tgcgtctcgc gctgcccgcg cccgacacgc gcgatgccgt ccgcgttggc
     3181 gcgctcgtgg gcgacccggt ccgggccggc atcctggcca tcctgcgcga cggcccgcac
     3241 tgcgtctgcg agatggccgc cgccctgggc gagcgccaga acaacgtcag caatcacctg
     3301 gctcgcctgc gcgaggccgg cctcgtgcgc gcgagccgcc acggtgccga tgcgcgctgg
     3361 atctactacg agcgcgacga ggcggcagtc gcggcagcgc tggaaatgct gacgtctcgg
     3421 atcggataga gccgcggcgg accgcaggct acccgcgctg gccgcgcatc agctgaatag
     3481 cgagcagctt gtcggcgtag ctgcgcctga tcgcgttgag atcctggatc ggcagatcca
     3541 gctcgttcgc gatctgtccc agctcggccg cttcctcgga cgtgatcgcg ccatcggctg
     3601 cggcaacggc atagcacgcc tccaccgccg cgcggcgctg ctcgctcgtc gagtgagcgg
     3661 caaactcgcg cgttaccagg tagtcctccg tgccgccgta catctcgccc tggttcttgg
     3721 caatctcgac gacgagcacc gcctgcgcct caggcaaacc gccgatctcg gccacgatct
     3781 gctccatcag ccgcgtctca tcgggagtga cgttcagatc cgcgtgggcg gcgcggctga
     3841 cgacataggc gaagccggcc aggatgcgcc gccgctctgg gtcgagctgc cccagctcgg
     3901 cgacaatgcg tcgcacggtc tgggtttccg actcgacacg ggtctcccca gcaccgggtg
     3961 acgccgcggc ctgcctgccc aggcccagga agcgcaacat tgccatgggt ggagcatgac
     4021 acagccgccg gaactctctg acgcgtgaga gcgtcaccgc gtccatgacc cgaaaaacca
     4081 gctctctggc gcttgccctc ctcgcaaccc tgctcgtcgc gtgcggaggc ccaggcgcaa
     4141 gcccaacccc gccaccgact cccacgccct ctcccacgcc atcgcctgcg cccgtcagcg
     4201 aggtgaccac ggccgcccag gctgccgccc tggtcttcgc ccacgagcac atcgcccaga
     4261 ttgggccatt gatgcccgac gtcatcggtc agtcctcgtg gtacgaggcc aatgaggagg
     4321 ccggtgggta cgcggtcaag atcaccgtcg gtgcgggcga ttgccaggcc ggctgcatcg
     4381 agcgccacac ctggacatac cacgtcgatc tcgacggaac ggtgacgctc atcggcgatg
     4441 aaggcgacga catcggcctg ccgaccgacg agggaacagc cgatcctttg tccctgcgga
     4501 tcacgctcag cgccggcccg gtttgcccgg tggagcagaa tccacccgat ccggcgtgcg
     4561 ccccacgacc ggtcgcgaac gtcgaggtgc tcgtgttcga cccaagcgga cagcaggtcg
     4621 gcgagggcgt gtccgacgac cacgggctga tcaatatgca gctcccagca ggcgcttact
     4681 acgtcgtcgc ggcgccggtc gagggcctga tgcgcaacgc cgagccgctt gcctttgccg
     4741 gcgtcggtgg tgacagcgtc gcgctcgtaa tgggctacga cacgggcatc cgctgagcgg
     4801 ccgtcgtgcg tcctagcggc gcacgaacgt cgccgtcacc gtcttggccg aacccatgcg
     4861 cacggtgcac gtccgctgcg tcccagcaca cgcgcctgcc cagctcttga accggtaacc
     4921 cggtgccggc gtggcggtca agcgcacact gcggcccacg acgaacgtgc tggcgcagtc
     4981 cttgccacag tcgatcttgg tggcgctgac ccgtccgcgg cccaccttgg acacggatag
     5041 cgcggccgtg ccgcgcagcg cgccggcctt ggccccccaa tcgccgtgct gcttggtcaa
     5101 gccgtcgctc gtcagctgcg tcggcagggc cgcggcggcc gcgaccggct gcccggcgct
     5161 cccaagcgcg gcgcgcggcg gcacgggcaa cgaatagagc tcgtagtggc ccgacacgcc
     5221 gctgcggaag atgacctggg tgccatcggg cgaccagctg ggttgatcct cgttctgatc
     5281 cgccgtcgcg gtgacgttgc gcaggtcggt cgggtcgttg gcgttgacca gccagatgtc
     5341 gaagttgccg ttgcgcgtgc tggtgaacgc gatcaggctg gaattgggcg accactgcgg
     5401 ggtgacgtcc tcgaacatgt cgcagttgaa gctctcgtcg aaggtgagct ggtgcatgtt
     5461 gtcgccaaag ccgtcgccat cggagtctgc cgaatccatg acccacagat cgcacgtgtc
     5521 aggatccagc ccgcggccgg tggaaacgaa agccagccgt gacccgtcgg gcgaccaggt
     5581 gacctgcgag tcctcgatgc ttccgccggt ttctggatca aaggcgtcgc ttgtcagggg
     5641 tgtggcattg gcgagcggtc cgacctcgcc cagggcatcc atgacgtaga tctcgtaccc
     5701 aaagtcctca tctaccccaa ccgtccgcga cgaggcgaag gcaatgcgcg tcccgttggg
     5761 cgaccacgcc ggctgcacgt cgtagccgtt ctgtgagtcg gacagaagct gctgatcgtc
     5821 agttgttgga tcggtgtcca tgacctggat gcggggtccg ccgagctcgc cccaaccgtc
     5881 gaaaacgatc ttgcttccat caggcgacca atcagggttg tcgttgtagg ccccatctgt
     5941 cgtcagccgg acgggcggca ggctgggttg cgccgggtcg acgacgtaga tgtcgtactc
     6001 gagcaggtca ttccacgacg agtaggcgat caggccgtta gggccggccg cggccaacgc
     6061 tccgggcgcg gttcctccag cgagggcaag gacgacggcg gacgcggcga gggctgccag
     6121 aaaaggtcgg cgcgggcggt tcattttgtt ccccaagaat ggtgcaggtt ttgcgacgtt
     6181 agatccgtga cctgggccaa atgagggtcg agtggcccct tgtggccgga ccaatcggcc
     6241 ctacgcgcca gcgccccacc gtggcgtgcc agcgatgtcg gtgacgcgac gcgtgactgg
     6301 ccgggcggcc gtaacctatg ccgagccatg actgccgaag ccgtcaaccc catcgtcgcc
     6361 accccccgcg cggacgcctc ggccactccc cgcagtccgg ccacgcgcga tctgggcatg
     6421 cgcgcgacgc tgctcttccc cacgcgcgag attgcccagc agtactacct gccgctcatc
     6481 tacaccgcgt gccggacgtg ctacagcgag ctcgagccgg ccgacatctt cgaccgagcc
     6541 acacgtgggc aggtcgcgac cgagaagcag caggacctca tccggcgggt gatcgagtcg
     6601 ggccacggct ccacgatcga gcacatcgtc ttcacctttg gcatcagcgg cgtcacgcgc
     6661 acgctgtcgc accagctggt ccgccatcgc gccggcgtcg cgttcgacca gcagtcgcag
     6721 cgttatctga actacaagaa gccctcatac atggtgcccg gctcgctcca ggacgcgccg
     6781 ccggagatgc gcgaccagtt cacctcgcag atggacgagt cgctggcctt ctacggcgag
     6841 atgctcgagg cgggcatccc cggcgaggac gcgcggttcg tgatgcccaa cgcgacgcgc
     6901 accaacctga tcatgactgc caacctgcgc tcgctgatcc acatgagtgg cctgcgcctg
     6961 tgcacgatgg cccaatggga gatccgccgg ctcttccagc tgatccgcca cgaggtcttc
     7021 agcgtgtcgc cgttcctggg ctcattcctc gcacccaaat gcgtcccgct gggctactgc
     7081 gacgaggacc gcaaccgcga cgagcactgc cccatccggc cgcacaagga caacgtgctc
     7141 gcggcgtggg ccgagaagaa ggcagccgcc aaggccgccg gtcgagagct gccgactctc
     7201 taggttcgcc cggcgctcgc ccggccggtc aggcaggggc caagcggcgt tcaggcagcg
     7261 gccaggcggc ggacgaagct ccggatcagc gcctggaagc gtgagccggc ctcagccgcc
     7321 gcctccagga cctcctcgtg cgagagcggg ttgccggtga tgcccgcgcc gggatttgtt
     7381 acgagcgaga cgcccacgac ttcagcgccc gcccagcgcg ccacgacggc ctccagcacg
     7441 gtggacatgc cgacggcgtc tgcaccgagc gtgcggagca tgcgcacctc ggccggcgtc
     7501 tcgtaggtcg ggccgatcag accggcatag acgccttcct cgagcgtcac gtcctcgtcc
     7561 gccgccgctt ggcgcatcag ctcattgagg cgcgggctcc aggcattgac gaggtcggga
     7621 aagcgcgacc cgagcgcgtc gtcgttcgcg ccgatgagcg gcgagcgccc ggtcagattg
     7681 agatggtcgg tgatcagcat gagcgttccc gccggccagt cgacgcggat gccaccggcc
     7741 gcgttgctga ggacgatcac ctgcgcaccc aggcggcgca tcagcaaaac gggctcgaca
     7801 acgagacgct ccgagttgcc ctcgtacaga tgcagacggc cctgcaggca gaccaccggc
     7861 acgccatcca gacggccgag caacagccgc ccggcgtggc ccggagcgga tgccgcgggc
     7921 caacccggaa actccgcgaa gggaaccgcc accgcgtccg tcagcgcgtc cgcgagcccg
     7981 cccagtcccg agccaaggac gatgccgacg cgtggcacga ggtcggtccg ggcgcggacg
     8041 gcgggctcga gcaggtcaag ccgctgggaa aacgacgggg tactcattcg cggagcataa
     8101 agaaacgccc ggagagacct gccgagcctc gtttcgcctt gatgtgatct cagcgctcga
     8161 gcgtctcgcg gcccctccga gcaaggacta cgttagggtc gggctactac c
//
LOCUS       DATBRX010000125         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_152449, whole genome shotgun sequence.
ACCESSION   DATBRX010000125 DATBRX010000000
VERSION     DATBRX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152449"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VIF08_04470"
     CDS             <1..279
                     /locus_tag="VIF08_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135273.1"
                     /translation="FFKFFMRKVFPRQVKRYEEEFGMKFIGWFNVAYGWDFDNVILLE
                     LPDYATIDKLESDPRARAIGHRAGEWMFERHHSMFLRERMGPELKYTP"
     gene            338..1216
                     /locus_tag="VIF08_04475"
     CDS             338..1216
                     /locus_tag="VIF08_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135274.1"
                     /translation="MSSALGEIATEAALERTSFLSAAGKQLHDFLQGNKARIKEAGGL
                     VLIDDDPDYLSVAPDGSFRSRTRYQDEVTGEWRSETEVIDTAAELVELYNPAEIFAAF
                     AEAAREQAGLPEEPTGAEDLMDVAGIAPDETVGVGIGGTDVTEEGYARAADEWAASQT
                     AQGAPANADEAASRLYDLALTFQERSQHTEARLLEQFETLVTDLSPFVGDLMVLDDED
                     ERLWLKRNGHFEAEVVPEADEDGAREGSWKRLSSPDDMVEFYDPTDVFGDLAEAIAES
                     FPHVTGDAESDSDSEA"
     gene            1238..2098
                     /locus_tag="VIF08_04480"
     CDS             1238..2098
                     /locus_tag="VIF08_04480"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135275.1"
                     /translation="MRLLDAELIESLEFLPGQWLQTYLAPLLATHSAAGQFVHVRTPD
                     YSGLVLRRPFSINTVDRVGGRITIHFRVTGVGTAWLARMQPTQQVEMLGPLGRGFEVD
                     PRSRHLLLVAGGLGMAGVRSLVDDALAADRSVTLLFGAASAMEVYPSSLLPDEVEYVV
                     ATDDGSFGHHGRVTELVPQYEAWADQCFACGPQPMLAAMAQLAASRQQRMGVARLGPR
                     RGRGTKAPPLGSRQARRRSWLQVSMEQNMGCAVGACLGCVIMGTEGPQRVCREGPVFA
                     AEEVAWEGWR"
     gene            2095..2754
                     /locus_tag="VIF08_04485"
     CDS             2095..2754
                     /locus_tag="VIF08_04485"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEY8135276.1"
                     /translation="MTARLVHYGPAMGFIGSAFFAIASLIAGVFYVGTQSEPFSPLNH
                     WVSELGEMGVSSLALVFNIGLFVGGLALALFFFALGRLRSSRLASVYVIVGIVAGVSG
                     ALVGVFPMNNRSIHIVFALGFFVLGWVAVGLASLDIWRRPEARFSRWLPALGALTVAV
                     FLLFLSVYIPYLYTGTGSDRPDVSIATVLEWLVLIGIMGWVLVASATWWRHGRGSRNT
                     A"
     gene            2732..>3312
                     /locus_tag="VIF08_04490"
     CDS             2732..>3312
                     /locus_tag="VIF08_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135277.1"
                     /translation="MAVATPPEAGSGVDLTVDLGRGLVLRNPFLVASGTFGYGTEYAD
                     VVDVERLGAICSKGTTLRPRQGNPPPRVTETPAGMLNSIGLQNPGVDGVLEKYGSTWA
                     SWKVPVIVNVAGESVEDYVEVARRLDGQPGVAGIELNISCPNVGAGGLQFALDAGAAS
                     EVTAAVRRATDLPLMVKLSPAATDVRGIARGIE"
BASE COUNT          507 a         1072 c         1161 g          572 t
ORIGIN      
        1 tttttcaagt tcttcatgcg caaggtcttc ccgcgccagg tcaagcgcta cgaggaagag
       61 ttcggcatga agttcatcgg ctggttcaac gtcgcctacg gctgggactt cgacaatgtc
      121 atcctgctcg aactgcccga ctacgccacc atcgataagc tcgagagtga cccgcgggcg
      181 agggcgatcg gccatcgcgc cggcgagtgg atgttcgagc gtcatcactc gatgttcctg
      241 cgcgagcgca tgggccccga gctgaaatac acgccgtaga tactcaatga caatcggacg
      301 cggaaaagac aagcgaaagg aggtcggaat ggccggcatg agcagcgccc tgggcgagat
      361 cgcaaccgag gcggcgctgg agcgaacctc cttcctatcg gcagcgggca agcagctgca
      421 tgactttctt cagggcaaca aggcacgcat caaggaagcc ggcgggctgg tcctgatcga
      481 cgacgacccc gactacctgt cggtcgcgcc ggacggcagc ttccgcagcc gcacgcgcta
      541 ccaggacgag gtcacgggcg aatggcggag tgagaccgag gtgattgaca ccgcggccga
      601 gctggtcgag ctctacaacc cggccgagat ctttgccgcg ttcgccgagg cggcgcgcga
      661 gcaggccggc ctgccggaag agccaaccgg agccgaggac ctgatggatg tcgcgggcat
      721 cgcgccggac gagacggtcg gcgtgggcat cggtggcact gacgtgaccg aggagggcta
      781 tgcccgcgcc gccgacgaat gggcggcgag ccagactgcg cagggcgcgc cggccaacgc
      841 ggacgaggcc gcgagccggt tgtacgacct ggccctcacc ttccaggagc gcagccagca
      901 cacggaggcg cgcctgctcg agcagttcga gacactcgtc accgacctgt cgccgttcgt
      961 cggtgacctg atggtgctcg acgacgagga tgagcgcctg tggctgaagc gcaatggcca
     1021 tttcgaggca gaggtcgtgc cggaggccga cgaggatggc gcgcgcgagg gctcgtggaa
     1081 gcgcctctca tcaccggacg acatggtcga gttctacgac ccgaccgacg tgttcggtga
     1141 cctggcggag gcgatcgccg agtccttccc gcacgtcacc ggtgacgccg agtccgactc
     1201 cgactctgag gcctaggccg gaccaggcgc gtaccacgtg aggctgctcg acgctgagct
     1261 gatcgagagc ctcgagtttc tgccggggca gtggctccag acctatctcg cgccgctgct
     1321 cgctacccat agcgcggccg gccagttcgt ccacgttcgg acgcccgact actcgggcct
     1381 cgtcctgcgc cggcccttct cgatcaacac cgtcgaccgg gtcggcggcc ggatcacgat
     1441 tcacttccgc gtgaccggcg tcggcactgc ctggctggcg cggatgcagc caactcaaca
     1501 ggtcgagatg ctgggtcctt tgggccgcgg ctttgaagtc gaccccagga gccgccacct
     1561 gctgctcgtg gccggggggc tcggcatggc cggcgtgcgc tccctcgtcg atgacgcgct
     1621 cgccgcggat cgcagcgtga cccttctgtt tggcgcggcg tcggcgatgg aggtctaccc
     1681 atcgagcctg ttgcccgacg aggtcgagta cgtcgtggcc accgacgacg gctcgttcgg
     1741 tcatcacggc cgcgtgaccg agctcgtgcc gcaatacgag gcgtgggccg atcaatgctt
     1801 cgcctgcggg ccgcagccca tgctggcggc gatggcccag ctggcggcga gccgccaaca
     1861 gcgaatgggc gtagcgcgac tcgggccacg ccgcggcagg gggaccaagg ctccaccatt
     1921 gggctcgcgg caggcgcgcc gccgctcttg gctgcaggtc agcatggagc agaacatggg
     1981 ttgcgccgtc ggggcgtgcc tggggtgcgt catcatgggt accgagggac cgcaacgggt
     2041 gtgccgcgaa ggccccgtgt ttgccgccga agaggtggcg tgggaaggtt ggcgatgacc
     2101 gcgcgactgg tgcactacgg gccggctatg ggcttcatcg gatcggcctt cttcgcgatc
     2161 gcgtcgctga tcgccggcgt gttctacgtg ggtacgcaga gcgagccctt ctcgccgctc
     2221 aaccactggg tctccgagct gggcgagatg ggtgtgtcca gcctggcgct ggtgttcaac
     2281 atcgggctgt tcgtcggtgg cctggcgctg gcgctgttct tctttgcctt gggcaggctg
     2341 cgcagctcgc gcctggcgtc ggtctacgtg atcgtcggca tcgtcgccgg cgtgtccggc
     2401 gccctggtgg gggtcttccc aatgaataac cgctccatcc acatcgtctt tgcccttggc
     2461 ttcttcgtct tgggttgggt cgccgtcggg ctggcgtcgc tcgacatctg gcgtcggcct
     2521 gaagcgcggt tctcgcgctg gctgcccgcc ctgggtgctc tgaccgttgc tgtcttcctg
     2581 ctgttcctgt cggtctacat cccgtacctg tacaccggca ccggtagcga tcggccggat
     2641 gtctcgatcg cgactgtcct cgagtggctc gtgttgattg gcatcatggg ctgggtgctc
     2701 gtcgcgtcgg cgacgtggtg gagacacggg cgtggcagtc gcaacaccgc ctgaggcggg
     2761 atcaggtgtc gacctgacgg tcgacctggg gcgcggcctg gtgctgcgca atcccttcct
     2821 ggtcgcgtcg ggcacgttcg gctacggcac ggagtacgcc gacgtcgtcg atgtggagcg
     2881 gctgggcgcg atctgctcca agggaacgac cctgcgacca cgacagggca acccaccgcc
     2941 gcgtgtcacc gagacgcccg ccgggatgct caactcgatc ggactgcaga acccgggcgt
     3001 cgacggcgtc ctcgagaagt acggctccac ctgggcatcg tggaaagtgc ccgtcatcgt
     3061 gaacgtcgcc ggtgaatccg tcgaggacta cgtcgaagtg gcgcgccgcc tcgatggcca
     3121 gcccggcgtg gcaggcatcg agctcaacat atcgtgcccc aatgtcggtg ccggcgggct
     3181 gcagttcgcg ctggatgccg gcgcggccag cgaggtgacc gcggccgttc ggcgcgccac
     3241 cgacctgccg ctgatggtca agctgtcgcc ggccgcgacc gatgtgcgcg gcatcgcccg
     3301 gggcatcgag ga
//
LOCUS       DATBRX010000126         9706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1492712, whole genome shotgun sequence.
ACCESSION   DATBRX010000126 DATBRX010000000
VERSION     DATBRX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492712"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VIF08_04495"
     CDS             <1..388
                     /locus_tag="VIF08_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEY8135278.1"
                     /translation="NQRDMAQEALDRWWGPLMQMHGPRTDPAKDRDLFWRIKEKTGEQ
                     LRQEFLSAYVPKLWEVGLYPPDPELKEDPETNEWRYTEPDWQELRSVVTGHGPKSQER
                     LDFRRLNHEQTAWVRQTILAPVGVAA"
     gene            385..735
                     /locus_tag="VIF08_04500"
     CDS             385..735
                     /locus_tag="VIF08_04500"
                     /inference="COORDINATES: protein motif:HMM:NF018002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135279.1"
                     /translation="MTSEQTVGEGHVDEAGRLGPGADPEVWEVFRQEKDGEAMRHAGN
                     VRAPDRELAQHYAREFFSRRNESVRLWVVKRTEISDLSDPDLLQPPLDRSFKRPGGYV
                     LRDKLDEARKRAQK"
     gene            732..1568
                     /gene="paaC"
                     /locus_tag="VIF08_04505"
     CDS             732..1568
                     /gene="paaC"
                     /locus_tag="VIF08_04505"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY8135280.1"
                     /translation="MTTETRRATRQLIQPDLPQFDASGLDPVLRAAIADLLLAMADDE
                     FVVGFRDSEWTGIAPMLEEDVAFSSLAQDEIGHARLLYQLRSALTGEDPDKVAFHRQP
                     NEYCHARLLDHPRQGWGFTIVRRWLYDTADSIRLAGLARSSFAPLAEVVAKIQREERY
                     HLMHLNAWLERLGTRPGKPRGKVLSALKQLNPIATSVFAPLSNEQMLVDAGVLSAPMV
                     QLAARWRSEANARLCALNLRPIPEGDPAADGRDHATPDESFVWLWNEFTSVARLEEGA
                     EW"
     gene            1562..2119
                     /gene="paaD"
                     /locus_tag="VIF08_04510"
     CDS             1562..2119
                     /gene="paaD"
                     /locus_tag="VIF08_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEY8135281.1"
                     /translation="MVAPTHNVSSAAAFGRAAREAAAPRRSGALLPSLAAIPLESAVW
                     AALHDVHDPEIPTISVVDLGVIGGVRTEEGRIRVELLPTFVGCPAINVLQDAIAERLR
                     EFADDVSVDVSFAEPWTTQRITADGRAALRAAGFAPPSTDETVECPYCRSRNTRIENI
                     FGPTLCRAIYYCPDCRQPFEQFKEV"
     gene            2119..2481
                     /locus_tag="VIF08_04515"
     CDS             2119..2481
                     /locus_tag="VIF08_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135282.1"
                     /translation="MIRDRFALAVSGLAATGLMTLAALAMNDAVPRNVDVTVLWLAGA
                     LTVGLVAAWVFVPRLPKWIVAGWGLILIALGVGVIISAPVPAGAGQFPWALIGLVVFG
                     IVIIVTAVVHSGSAGTDH"
     gene            2510..3013
                     /locus_tag="VIF08_04520"
     CDS             2510..3013
                     /locus_tag="VIF08_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HEY8135283.1"
                     /translation="MTNTEPPFTVTFELTTEDLVDYLRVAQKSLNTIGMAAGVVGMLY
                     GAYMAWTGDVVVGGVLVAMGAFLILVSATRYADRLRARSVGKRIIGTQATITFDDGGI
                     ASSTAGGSGHASWAAVDNIMESPETLVLRRGRLTKLWLPKRALGTPAERDALLDYIRA
                     HVSRPGA"
     gene            complement(3010..4548)
                     /locus_tag="VIF08_04525"
     CDS             complement(3010..4548)
                     /locus_tag="VIF08_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8135284.1"
                     /translation="MSSPSLPWFLAGVAAAAVAIGVGELIGGVLGGSSIVAAIGALVI
                     ALQPPWGKDLMAQLFGTNDKAMLEATVAIGGLLVGGVLGLAAKREERIAIAGFAAFGL
                     IAFVLLLQDPLNSLPASVIVALVATAGGLITMSWLAGAIRRFAASTNATTDLGRRGFV
                     ALAALLLVGGSGLTVVGRLLGGQVGNTSSTPPGPLPKPRETVGPILATADFSTDPALA
                     GISPIVVPNEDFYRIDTRLNTPRVDSATWSLRIHGMVDREISLSYDQLLARPLVERYV
                     TIACVSNQVGGPLVGNAKWTGTSLIDLLNEAGIQAGATQVVGRSFEGWTSGFPTEHLS
                     GAGRDAMVVVGMNDAPLPPQHGFPARLIVPGLFGYVSATKWLAEIELTTLEAFDAYWI
                     PLGWAKEGPILTQSRIDRPMNGAIVTLGNYEVAGVAWAPTRGVSRVEIRIDDGEWQQA
                     QLSQPLSNYAWLQWRATVDAVEGEHTLTVRATDGNGETQPEARTAPAPSGARGWHQVR
                     FFVR"
     gene            4775..4945
                     /locus_tag="VIF08_04530"
     CDS             4775..4945
                     /locus_tag="VIF08_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135285.1"
                     /translation="MELFAASGAMAVPHEADLLEHVEGPVHGRWDRLGIGRATALYQL
                     AGRNVPARPREH"
     gene            5098..6027
                     /locus_tag="VIF08_04535"
     CDS             5098..6027
                     /locus_tag="VIF08_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135286.1"
                     /translation="MPDAGTIALLLTALVLGLRHGIDWDHIAAITDITSTTSAAEAAE
                     VEHEEVHELGQGHAHEHGGEAELRTHDAELAAHTAHDPHTPVRHRSLLALERQPVMLG
                     TLYALGHGTTVAILGAIALTFGALLPDWVDPLMGRIVGLTLVLLGIFVFVSLYNYARH
                     GSEFRLRSRWMLVFDGVRYGWRRFQGWLHGHTHAEPLEMSSYGPRTAFLVGMIHGIGA
                     ETGTQILFIAAIGGANAVGLGIPMMVAFIVGLLITNTLMVLISSSGFLASQSRRQIYV
                     VIGAIAGIFSLVVGFAFLFGIEDVLPSLEHLFG"
     gene            complement(6358..7518)
                     /locus_tag="VIF08_04540"
     CDS             complement(6358..7518)
                     /locus_tag="VIF08_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8135287.1"
                     /translation="MSLINLRRLRRVAATATLLTLVATATPSPAMAAVGLQFVAGGFS
                     SPVFVTHAGDSRLFVVEQTGRIKIVGGGTFLDIHTLIRTSGGEQGLLGLAFHPNYASN
                     GLFYVNYTRKSDGDTVIAEFKRSTGNPDVANAASKRVVLRINQPFANHNGGWMAFKGN
                     YLYISTGDGGGGGDQKNRAQNLDKLLGKILRINPLDPPGAATYSIPSDNPFVGVAGKD
                     AIWSYGLRNPWRCSFDANDGSLYCGDVGQEKFEEIDHVGTGRGVNFGWRKLEGNHYYN
                     WSGHTSGTLCTGSCFTLPIAEIPHSEAGDDNCAITGGYVARRSGAPMFGHYVAGDFCS
                     GKIWVFPSTFSTGDPLPVPDDTSYNISSFGEGNDGKLYLVDLNGSIYRLTDS"
     gene            7655..>9706
                     /locus_tag="VIF08_04545"
     CDS             7655..>9706
                     /locus_tag="VIF08_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8135288.1"
                     /translation="MSFEFHVSRDARQRYEFDEALFGLSGNVVFAGYPAARRFAQAMN
                     AQRDVAADPGRAVRAGDINAMGLVDEILHHVVELYRRQRNPAAMAGALAALDQSLGSE
                     ALETALTVFAERFPTVAAYRGEQSAADYLAGEIGGVPNRQIALEELLLVWLANGNPAF
                     GRYEELFDDTPLADGSAYAQLIDGLRHYFRSQPGFGPDGDDLISLLQRPAMEAPGSLA
                     DQLRWISSRWGFVAERFGDVVTIGLDVLREDERAEWMRFNPSDGGTAVFGGGAEGAEA
                     ESGALHGFGEAADAEVERFSQDLDWMPRVTLLAKSTYVWLDQLSRSYGRPIYRLDQIP
                     DEELDRVAASGFTGLWLIGLWERSRASQQIKQLRGNPEAVASAYSLMDYRIADDLGGD
                     AAYANLRDRAWARGIRLASDMVPNHMGIDSRWVVEHPDWFVQRTDPPYPAYTYSGPNL
                     SSDERVGIYIEDHYFDNSDAAVTFKRVERRSGEERFMYHGNDGTSMPWNDTAQLDYLR
                     ADVREAVIQTILHVARQFPIIRFDAAMTLAKKHIQRLWYPEPGEGGAIPSRAESAMTK
                     AQFDAAMPQEFWREVVDRVAAEAPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNML
                     RDERNAEYRTVIKNTLEFDPQILKRYVNFMNNPDERTAVDQFGKGDKYFGVATLMATM
                     PG"
BASE COUNT         1558 a         3250 c         3308 g         1590 t
ORIGIN      
        1 gaaccagcgc gacatggccc aggaggcgct cgatcggtgg tggggaccgc tgatgcagat
       61 gcacggcccg cgcaccgacc cggccaagga tcgcgacctg ttctggcgga tcaaggagaa
      121 gaccggcgag cagctgcgcc aggagttcct gtcggcgtat gtgccgaagc tgtgggaggt
      181 gggcctttac ccgcctgacc cagagctgaa ggaagatccc gagaccaacg aatggaggta
      241 caccgagccc gactggcagg agctgcggtc ggtggtcacc ggccatggtc ccaagagcca
      301 ggagcggctc gacttccgcc ggctcaacca tgagcagacg gcgtgggtcc gccagacgat
      361 cctcgcgccg gtcggggtcg ccgcgtgacg agcgagcaga cagtcggcga aggccacgtc
      421 gacgaggccg gccgactggg cccgggtgcc gatcccgagg tgtgggaggt cttccgccag
      481 gagaaggacg gcgaggcgat gcgccatgcc ggcaacgttc gcgcgcccga ccgcgagctg
      541 gcgcagcact acgcgcgcga gttcttcagc cggcgcaacg agagcgtccg actatgggtc
      601 gtcaagcgga cggagatctc cgacctgagc gacccggacc tgctccaacc gccgctcgac
      661 cggtccttca agagaccggg tggctacgtg ctgcgcgaca agctcgatga ggcgcgcaag
      721 cgggcccaga aatgacgacg gaaacacggc gcgcgacgcg ccagctgatc cagcccgacc
      781 tgccgcagtt cgatgcgtct ggcctcgacc cggtgctgcg cgcggccatc gccgatctcc
      841 tgctggccat ggccgacgac gagttcgtcg tgggcttccg cgactcggaa tggaccggca
      901 tcgcgccgat gctcgaggag gacgtcgcgt tctcgtcgct cgcgcaggac gagatcggcc
      961 atgcccggct gctgtatcag ctgcgctcgg cgctcaccgg cgaggatccc gacaaggtcg
     1021 cgttccatcg ccagcccaac gagtattgcc acgcgcgcct gctcgatcac ccgcgtcagg
     1081 gctggggctt cacgatcgtg cgccgctggc tgtacgacac ggccgactcg atccgcctcg
     1141 ccgggctggc acgctccagc ttcgcgccgc tggccgaggt cgtggccaag atccagcgcg
     1201 aggagcgcta ccacctgatg cacctgaacg cgtggctcga gcgcctgggc acgcgccccg
     1261 gtaagccgcg cggcaaggtg ttgtccgcgc tcaagcagct caatcccatc gcgacatccg
     1321 tgttcgcgcc gctgagcaac gagcagatgc tcgtcgacgc gggtgtgctg tcggcaccga
     1381 tggtccagct ggcggcccgc tggcgatcgg aggccaacgc ccgcctgtgc gctcttaacc
     1441 tccgaccgat acccgagggc gaccccgccg cggacggacg cgaccacgcg acccccgacg
     1501 aatcgttcgt ctggctgtgg aacgagttca cctccgtagc ccgcctcgag gagggcgcgg
     1561 aatggtagcc ccgacccaca atgtctcctc cgctgcggcc ttcggccgag ctgcgcgcga
     1621 agcggcagcc ccccgccgtt ccggcgcgtt gctgccgtcg cttgcggcga tacccctcga
     1681 atcggccgtc tgggccgcgc tgcatgacgt gcacgatccc gagatcccga cgatctccgt
     1741 cgtggacctg ggcgttatcg gcggagtccg gacggaggag gggcgcattc gggtcgagct
     1801 gcttccgaca ttcgtcggct gcccggcgat caacgtcctc caggacgcga tcgcagagcg
     1861 gctgcgcgag ttcgccgacg atgtgagcgt cgatgtcagc ttcgccgagc cgtggacaac
     1921 gcagcgcatc accgcggacg gtcgcgcggc cctgcgcgca gccgggttcg cgccacccag
     1981 caccgacgag acggtcgagt gcccgtactg ccgttcgcgc aacacgcgca tcgagaacat
     2041 cttcggccca accctgtgcc gcgcgatcta ctactgtccg gattgccgcc aaccattcga
     2101 gcagttcaag gaggtctgat gatccgcgac cggtttgccc tcgcagtgag cggtttggcc
     2161 gcgaccggat tgatgacgct cgccgcgctg gcgatgaacg acgccgtgcc gcgcaacgtg
     2221 gacgtcacgg tgttgtggct ggcgggggcg ctgaccgttg gcctggtcgc ggcgtgggtc
     2281 tttgtgccgc gcctgcccaa gtggatcgtg gccggctggg ggctgatcct gatcgcactc
     2341 ggagtcggag tgatcatcag cgcgccggtt ccggctgggg cgggccagtt cccgtgggcg
     2401 ctgatcgggc tggtcgtatt cggcatcgtg atcatcgtca ctgccgtggt ccattctggg
     2461 agcgccggca cggaccactg acgccgcggg tcgactctag gatgcgcagg tgaccaatac
     2521 cgagccgccg ttcaccgtga cctttgagtt gaccaccgag gatctcgtcg attacctgcg
     2581 ggtggcccag aagtcgctca acaccatcgg catggcggcc ggggtcgtgg gcatgcttta
     2641 cggcgcgtac atggcgtgga ccggcgatgt ggtggtcggc ggcgtactcg tcgcgatggg
     2701 cgctttcctg atcctggtgt cggcgacgcg ctacgccgat cgcctgcgag cacggtcggt
     2761 gggcaagcgc atcatcggga cgcaggcgac gatcacgttc gacgatgggg gcatcgcgtc
     2821 gagcaccgcg ggcggctcgg gccacgcctc atgggcggcc gtcgacaaca tcatggagag
     2881 ccctgagacg ctggtcctgc gacgcggccg gctgaccaag ctgtggttgc ccaagcgggc
     2941 gctgggcacg ccggcggagc gcgacgccct gctcgattac atccgcgccc acgtcagccg
     3001 gccgggggcc tagcggacga agaaccggac ctggtgccag ccgcgcgcgc ctgagggcgc
     3061 aggtgcggtg cgggcttcgg gttgggtctc gccattgccg tcggtcgcgc gaacggtgag
     3121 cgtgtgctcg ccttccaccg cgtcgaccgt ggcgcgccac tgaagccagg cgtagttcga
     3181 cagcggctga gacagctgcg cttgctgcca ctcgccgtca tcgatccgta tctcgacccg
     3241 cgacacgccg cgcgtgggtg cccaggccac gccggcgacc tcgtaattgc ccagcgtgac
     3301 tatcgcgccg ttcatcggcc ggtcgatgcg cgactgggtc aggatgggac cctctttcgc
     3361 ccagccgagc gggatccagt aggcgtcgaa cgcctcgagc gtagtcagct cgatctcggc
     3421 cagccacttg gtcgcgctca cgtagccgaa caggccgggc acgatgagcc gcgccgggaa
     3481 gccgtgctgc ggcggcagcg gcgcatcgtt catgccgacg acgaccatcg cgtcgcggcc
     3541 ggcgcccgac aggtgctctg ttggaaaacc gctcgtccag ccctcgaagg agcggcctac
     3601 cacctgcgtc gcgccggcct ggatgcccgc ctcgttcagc aggtcgatga gtgacgtgcc
     3661 cgtccacttg gcattgccga cgagtgggcc gccgacctgg ttcgagacgc atgcgatcgt
     3721 cacgtagcgc tcgacgagcg gccgcgccag cagctggtcg tagctcaggc tgatctcgcg
     3781 atcaaccatg ccgtggatgc gcagcgacca tgtggcgctg tcgacgcgcg gcgtgttgag
     3841 gcgcgtatcg atcctgtaga agtcctcgtt cggcacgacg atcggggaga ttccggcgag
     3901 cgccggatcg gtcgagaagt cggccgtggc gagaattggg cctacggtct cgcgtggctt
     3961 gggcaacggt cccggtggcg tgctcgaggt gttcccgacc tggccgccga gcagccggcc
     4021 aaccacggtc agcccactgc caccgaccaa cagcaatgca gccagggcga cgaacccacg
     4081 cctgcccagg tcagttgtcg cgttggtcga cgccgcgaac cggcggatcg cgccggccag
     4141 ccagctcatc gtgatcagcc cgccggcagt cgccaccagc gcgacgatca cggacgcggg
     4201 caatgagttc agcgggtctt ggagaagcaa cacgaaggcg atcaacccaa acgccgcgaa
     4261 gccagcgatc gctattcgtt cctcgcgctt ggcggccagc cccagcaccc cgcccacgag
     4321 gagaccgccg atcgcgacgg tcgcctcgag catggccttg tcgttggtgc cgaagagctg
     4381 ggccatgagg tccttgcccc acggcggctg gagcgcgatg accagtgccc cgatggcggc
     4441 gacgatcgac gacccgccca gcaccccgcc gatcagctcg cccacgccga tcgcgaccgc
     4501 ggcagcggcg acccccgcga ggaaccaggg caggctgggc gagctcatca cacgacgcat
     4561 ctcagtgagg ttacgtcgcg cggcgaccta cggatcgggt cttctcggca cactccctgc
     4621 acacacccac cacagtcagg tgtgaaaggt ctgccctgaa gccgcgccgc gactcaaggc
     4681 tgccagtaag tgcagcggcc tcggtggcgt ctatctccca catcccatcg cacgcgcggc
     4741 agtgcagatg gccgtgctcg gttcgcggca gcacatggaa ctcttcgcgg cctccggcgc
     4801 catggccgtg ccgcacgagg ccgatctcct cgagcacgtc gagggtccgg tacacggtcg
     4861 atgggatcgt ctggggatcg gccgcgcgac agcgctctat cagctcgctg gccgtaacgt
     4921 gcccgcgcga ccgcgcgagc actgagagta ggagccgtcg ttgcggtgtc caacggaggc
     4981 cgcgcgcccg gagttcgtcg cgcgcggttg cccacgttgc cctcgtcatg tccggctcag
     5041 ggtaccactg gaattgtggg ttgttgctat tgcaacttac tgcaataatg cgcgcgcatg
     5101 cccgacgccg gaaccatcgc gctgctattg actgccctcg tgcttgggct gcgccatggc
     5161 atcgactggg atcacatagc ggcaatcact gacatcacca gcacgaccag tgcggcggaa
     5221 gccgcggagg tcgagcacga ggaagtgcac gagctgggcc agggccatgc tcacgagcac
     5281 ggcggcgagg ccgagctgcg cacccacgac gcggaattgg cggcgcatac ggcgcacgac
     5341 ccgcacaccc ctgtcaggca ccgctcgctg ctcgctttgg agcggcagcc cgtcatgctg
     5401 ggcactctgt acgcgctggg ccacggcacc acggtggcga tcctgggtgc catcgcgctg
     5461 acgttcgggg cgctgctgcc ggactgggtc gatccgctca tgggccgcat cgtcggcctg
     5521 accctggtgc tgctcggcat ctttgtcttc gtgtcgctct acaactacgc ccgccacgga
     5581 tccgagttca ggttgcgcag ccgctggatg ctcgtcttcg acggggtgcg ctatggctgg
     5641 cgccgcttcc agggctggct gcacggccat acccacgccg agccgctcga gatgagctcg
     5701 tacggcccgc gcaccgcctt tctcgtgggc atgatccacg gcattggcgc cgagaccggc
     5761 acgcagatcc tgttcatcgc tgccatcggt ggcgcgaacg cggtcggact ggggataccg
     5821 atgatggtcg cgttcatcgt gggcctgctg atcaccaaca cgctcatggt gctgatctct
     5881 tcgtcgggct tcctggcgag ccagagccgg cgccagatct acgtggtcat cggtgcgatc
     5941 gcgggcatat tcagcctggt cgtcggcttt gccttcctct tcggcatcga ggacgtgcta
     6001 ccgagcctgg aacacctctt cggctaagcg cgacccagcg cgcggacttg gccaaatgca
     6061 cgtggcgcgg tcgttattgc cgcacccggc caccttgccg acggcttggc taccaacggg
     6121 cctctcttcc gcgcaggcgc tggcttcgca ggtggccata cgacctgcat tggcgcacaa
     6181 ggccaggtcc accgcctcgc agttcgccta aggcgcgccg gacgcgcgtt aggcaggttc
     6241 gcgcgtcatc cggcgcggtt agtgactgct aggcgatcgt gtggccgtct tcacgcccga
     6301 gccgcgccat aaaggctggc acgaaagctt ttggcgggat tgcggctcca gcctgttcta
     6361 gctgtcggtc aggcggtaga tcgagccgtt cagatcgacc aggtacagct tgccgtcgtt
     6421 gccctcaccg aacgagctga tgttgtagct cgtgtcgtcg ggcacgggca gggggtcgcc
     6481 ggtggagaag gtcgacggga agacccagat cttgcccgag cagaagtcgc cggcgacata
     6541 gtggccgaac attggcgccc ccgacctgcg ggcgacgtag ccgccggtga tggcgcagtt
     6601 gtcgtcgccg gcttcactgt gcggaatctc ggcgatgggc aaggtgaagc acgagcccgt
     6661 gcacagagtg ccggaggtat ggcctgacca gttgtagtag tggttgccct cgagcttgcg
     6721 ccagccgaag ttgacgccgc ggccggtgcc cacgtggtcg atctcttcga acttctcctg
     6781 gccaacgtcg ccgcagtaca ggctgccgtc gttggcgtcg aacgagcagc gccacgggtt
     6841 gcgcaggccg tatgaccaga tcgcgtcctt gccggcaacg ccgacgaacg ggttgtcgga
     6901 cgggatcgag taagtcgctg ctccgggggg atcgagcggg ttgatgcgca gtatcttgcc
     6961 cagcagcttg tcgaggttct gggcgcggtt cttctggtcg ccaccgcccc cgccgtcgcc
     7021 agtcgagatg tagaggtaat tgcccttgaa ggccatccag ccaccgttgt ggttggcgaa
     7081 gggctggttg attctcagga cgacgcgctt gctcgccgcg ttggccacgt cagggttgcc
     7141 cgtggagcgc ttgaactcgg cgatgaccgt gtcgccgtcg ctcttgcgcg tgtagttgac
     7201 gtagaaaagg ccgttgctgg cgtagttggg gtggaacgcc aagcccagca ggccctgctc
     7261 gccgccgctc gtccggatca gtgtgtggat atcgagaaac gtgccgccgc cgacgatctt
     7321 gatgcggccg gtctgctcga ccacgaacag acggctgtcg ccggcgtggg taacgaaaac
     7381 cggcgagctg aagccgccgg ccacgaattg cagaccaacg gcggccattg ccggcgacgg
     7441 tgtcgccgtc gccacgagag tgagcagtgt ggctgtggcg gcgacgcggc gcaggcggcg
     7501 caggttgatc agggacatct cgcagcgacc tcattcacgg cgggcgggcg ggtagcgctg
     7561 accctaccaa aaaagccggg gtggccgatc ggccgatggt cccacagggg gaggggtgga
     7621 ccgatggccc cccgtcggtc aggattgcag tcagatgagc ttcgagttcc acgtttcgcg
     7681 cgacgcgcgg cagcgctacg agttcgacga agcgcttttc ggcctgtcgg gcaacgtggt
     7741 ctttgccggt taccctgccg cgcggcgctt cgcccaggcc atgaacgccc agcgcgacgt
     7801 cgccgcagat cctgggcgcg cggtcagggc aggcgacatc aacgccatgg ggctcgtcga
     7861 cgagatcctg catcacgttg tcgagctcta ccgccgtcag cgcaacccgg ccgcgatggc
     7921 cggggccctg gccgcgctgg accagagctt ggggagcgag gccctggaaa ccgctttgac
     7981 cgtcttcgcg gagcgcttcc cgacggttgc cgcctaccgc ggcgagcagt ccgcggccga
     8041 ctatctcgcg ggcgagatcg gcggcgtccc caaccgccag atcgcgctcg aggagctgct
     8101 gttggtctgg ctggccaatg gcaatccggc attcggccgg tacgaggagc tgttcgacga
     8161 cacgccattg gccgacggca gcgcgtatgc ccagctgatc gacgggctgc gccactactt
     8221 ccgcagccag cccggctttg ggcccgacgg cgatgacttg atctcgctcc tgcagcggcc
     8281 cgccatggag gcgccgggat cgctcgccga ccagctccgc tggatcagca gcaggtgggg
     8341 cttcgtcgcc gagcgcttcg gcgatgtcgt gaccattggc ctggacgtct tgcgcgagga
     8401 cgagcgcgcg gagtggatgc gcttcaaccc ctctgatggc ggaacagctg tcttcggcgg
     8461 cggcgcagaa ggggcagaag cggagagcgg cgcgctgcac ggcttcggcg aagctgccga
     8521 cgcggaagtt gagcgcttca gccaggatct cgactggatg ccgcgggtca cgctgctggc
     8581 caagagcacc tatgtctggc tcgaccagct gtcacgttcc tatggccggc ccatctatcg
     8641 gctggaccag atccccgacg aggagctcga cagggtagcc gcgagcggct tcaccggcct
     8701 gtggctgatc ggcctgtggg agcgcagccg cgcctcacag cagatcaagc agctgcgcgg
     8761 caacccggag gctgtcgcat ccgcgtactc gctgatggac taccgcatcg ccgacgacct
     8821 gggtggcgac gcggcctatg ccaacctgcg cgaccgtgcc tgggcgcgcg gcatccgcct
     8881 cgcgtcggac atggtgccca atcacatggg catcgactcg cgctgggtcg tcgagcatcc
     8941 cgactggttc gtccagcgca ccgatccgcc ctacccggcc tacacatata gcgggccgaa
     9001 cctctcgtcg gacgagcgcg tcgggatcta catcgaggat cactacttcg acaacagcga
     9061 cgccgccgtg acgttcaagc gcgtcgagcg caggagcggc gaagaacgct tcatgtacca
     9121 cggcaacgac ggcacatcga tgccctggaa cgacaccgcg cagctcgact acctgcgggc
     9181 cgacgtccgc gaggcggtga tccagacgat cctccacgtc gcccgccagt tcccgatcat
     9241 ccgtttcgac gccgcgatga cgctcgccaa gaagcacatc cagcggctct ggtatcccga
     9301 gccgggggag ggtggcgcaa tcccgtcgcg cgccgagagt gctatgacca aagcgcagtt
     9361 cgacgccgcg atgccgcagg agttctggcg cgaggtcgtg gaccgcgtcg ccgcggaggc
     9421 gccggacacg ctgctcctgg cggaggcgtt ctggctcatg gagggctact tcgtccgcac
     9481 cctgggcatg catcgcgtct acaacagcgc gttcatgaac atgctccgcg acgagcgcaa
     9541 cgccgagtac cggacggtca tcaagaacac gctcgagttc gacccgcaga tcctcaagcg
     9601 ctacgtcaac ttcatgaaca accccgacga gcgcacggcc gttgatcagt tcggcaaggg
     9661 cgacaagtac ttcggcgtcg cgaccctgat ggcgacgatg cccggg
//
LOCUS       DATBRX010000127         6028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3057765, whole genome shotgun sequence.
ACCESSION   DATBRX010000127 DATBRX010000000
VERSION     DATBRX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3057765"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VIF08_04550"
     CDS             complement(<1..769)
                     /locus_tag="VIF08_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8135289.1"
                     /translation="MGMLDELGQAMAAVAGKAGASVVHVGGGWRGGSGVVIGDGLVLT
                     NAHNVRGDGALVHFADGRRADAELKGVDVDGDLAVLAVDTAGTPALAWSRVSAAIGTP
                     VFAVAAADGGARVTMGFISSVARAFRGPRGRRISGSLEHTAALAPGSSGSALVDADGR
                     LVGLNTNRLGGGFYLALPADESLQNRVAGLGRGESAERPRLGIGIAPSWVARRMRRAV
                     GLPERDGVLVREVEDGSPAAAAGIQEGDLLIEAAGRPI"
     gene            851..1282
                     /locus_tag="VIF08_04555"
     CDS             851..1282
                     /locus_tag="VIF08_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8135290.1"
                     /translation="MSRANMAGVSGSGLDAALARVGDRWSLLVVEALLDGPRRFNELG
                     TAVAGIAPNILTDRLRRLERAGVLVGQRYSERPVRLAYSLTADGQELASVLRLLAAWG
                     SGEEGEGLRHLTCGTALEARWWCPTCSVAIGEDEASSLRYA"
     gene            1310..2245
                     /locus_tag="VIF08_04560"
     CDS             1310..2245
                     /locus_tag="VIF08_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY8135291.1"
                     /translation="MTGERARAALIVNPTSGGGRGWRVVGQAAPLLRQAGLVPEVVMC
                     TNGDEPGVLARQVAAAGHRLVVAVGGDGQAAAVAAGLVGSDTTFAMLPAGSANDYART
                     LGMPRGNVAGAVDAIAAGRTAHVDTIRVKVAGRERYFLNVVGTGFDAGVAARAERIPL
                     FRGAGRYVLALIAELPRFKAATITLDIDGATRELRAMMVAIANGVSYGGGMRVAPSAT
                     LDSGRLEVCVVGEISKPEFLRAFPRVFRGTHVTHRAVTMLSGREVRLAADRPLRLIGD
                     GEWFGELPATVTVVPRSLRVVVGPGYRGAAVSGSE"
     gene            2339..4909
                     /gene="pepN"
                     /locus_tag="VIF08_04565"
     CDS             2339..4909
                     /gene="pepN"
                     /locus_tag="VIF08_04565"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY8135292.1"
                     /translation="MSQTPVLTSPVAARDVLTEEEAQQRATRVSDTSYDIALDLVAGQ
                     STYRGDVTIRFNTSGAGPLFLDFRGREIELLEVNGLHIDPDWTGYRLTLPAEAVSGSM
                     AVHVVYVNDYDTTGDGFHRFVDPEDGAEYVYTNFEPYEAHRLYPCFDQPDIKARYEFS
                     VTAPAEWAVFSNAPIDSTEDAGAGRTLHRFARTELFSSYLTALIGGAYVHRSTEHNGV
                     SLRLYARASMERYLDDQAPEIFEITTQGMDFYSDLFAQPYPFGVKYDQVFVPEYNSGA
                     MENVACVTYNEAYLFRDPPTDNERLDRAETFLHELAHMWFGNLVTMRWWNDLWLNESF
                     ATYISYLAMTSATRFRNAWKVFNYRIKRWAYQTDQLPTTHPIAGTAADTEIAFLNFDG
                     ITYGKGASVLKQLAKYIGLDSFRDGLRLYFRRHAWGNATLADFLACLEEAHGASLENW
                     SEKWLRTASVNTIAAKWHAADGKIADVEIEQTAPADHPTLRPHAFEIALINEGATGLA
                     VESQPAWIDAAQTDLPELRGHAAPSLVFPNYGDHAYAKVALDERSLDFVRANLSRVDD
                     DLLRELLWMSLWEMVRDRQLRSTEYLAIARERIGSEPDRDILDMAVERAALALARFVP
                     ESRRTDEAHLWFEAALELLGAAKEADHQILWARSAVAVAASADDVARLATATAKDEPV
                     TGFVLDQEMRWLVAIKAVAFGLPNADELLAMEAARDRSDRGRRAMLRAEASRPTEAAK
                     AEAWERINGEGYGSFHLTRAAMQGFYWPHQKRLLETYEDRFFDQVRTVFETRDHPFAR
                     SYLLSLYPAYMGEPDVLERSHGLLSQLNGSLPTLTRQLTEAADDLDRVIKVRAFADKG
                     "
     gene            4914..5609
                     /locus_tag="VIF08_04570"
     CDS             4914..5609
                     /locus_tag="VIF08_04570"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8135293.1"
                     /translation="MTGRVWVEPVTLEGTYVRLEPMSLDHLPGLTQVGLDAEIWRWMP
                     MFVQAPGDMRTLVEEAIAEAEAGTMVPFTTIERATGKPVGSTRYLSIVPQHRRLEIGW
                     TWLAPAWQHSPVNTEAKLLMLRHAFEKLGALRVEFKTDALNEKSRTALLKIGAVEEGV
                     FRHHMVMPGGRQRDSAYYSVIEEEWPRVHQHLEARLTRLVRAAEQTAPPVADASEAVE
                     APELIEAPGSDRG"
     gene            complement(5606..>6028)
                     /locus_tag="VIF08_04575"
     CDS             complement(5606..>6028)
                     /locus_tag="VIF08_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY8135294.1"
                     /translation="DRTQPERERALAGFKSGEVPILVATDVAARGLDIEALPLVINYE
                     LPHTPHDYVHRIGRTGRAGATGQAISLVAPDEEDYLRAVNRLLKKDLPVRVVQGFEPD
                     LAAPRARPARSGRTSRSTTTRPVRRPGPPSRRGSTRVA"
BASE COUNT          928 a         2120 c         2059 g          921 t
ORIGIN      
        1 cgatcggccg gccggccgcc tcgatgagca ggtcgccctc ctggataccg gccgcggctg
       61 cagggctgcc gtcctcgacc tcgcgcacca gcacgccatc ccgctccggc agcccgaccg
      121 cgcggcgcat ccggcgcgcg acccacgacg gggcgatgcc gatgcccagg cgcggccgtt
      181 ccgcgctctc gccgcgaccc agtcccgcca cgcgattctg gagcgactca tctgcgggca
      241 gcgccaggta gaagccaccg cccaggcgat tcgtgttgag cccaaccagc cgcccgtcgg
      301 catcgaccag ggcactgccc gacgagccgg gcgctagcgc ggccgtgtgc tcgaggctgc
      361 ccgagatgcg ccggccgcgc ggaccgcgga aggcgcgcgc aacgctcgag atgaagccca
      421 tggtcacgcg cgcgccgccg tcggcggcgg ccacggcgaa gaccggcgtc ccgatcgcgg
      481 cactgaccct ggaccacgcc agggcagggg tacccgcggt atcgacggcc aggaccgcca
      541 ggtcgccgtc gacgtcgacg cccttgagct ctgcgtctgc ccggcgaccg tccgcgaagt
      601 gcacgagcgc gccgtccccg cgcacgttgt gcgcgttcgt caggacgagc ccatcaccga
      661 tcacgacgcc cgatccaccg cgccagccgc cgccaacgtg aaccaccgac gctccggcct
      721 tcccggccac cgctgccatc gcctgtccca gctcatcgag catgcccatt cggatccaac
      781 tccctgtttc tgtcgtgagt aacttgcgag ttgcaagtta ccatcgtcac gtaaccagcg
      841 caaaccggga gtgagccgtg ccaacatggc tggcgtgagt gggtcagggc ttgatgcggc
      901 actggcacgg gtcggcgacc gctggtcgct gctcgtcgtg gaggcgctgc tcgacggccc
      961 gcgccgcttc aacgagctgg gcaccgccgt agccggcatc gcccctaaca tcctgaccga
     1021 ccgcctgcgc cggcttgagc gcgccggcgt tctcgtgggc cagcgctact cggagcgtcc
     1081 tgtccgcctt gcctattcgc tcaccgccga tggccaggag ctcgccagcg ttctgcgcct
     1141 gctggctgcc tggggcagcg gcgaggaggg agaaggcctg cgccacctca cctgcggcac
     1201 ggcgctcgaa gcgcgctggt ggtgtcccac gtgcagcgtg gccatcggcg aggacgaggc
     1261 atcgagcctg cgctacgcgt gagcccaccg ctcacatagc atccgcgccg tgacgggtga
     1321 gcgagcgcgg gcggcgctga tcgtcaatcc aacgtcgggt ggtgggcggg gctggcgcgt
     1381 ggtcggccag gctgcgccgc tgctgcgcca ggcaggcctc gtgcccgagg tcgtcatgtg
     1441 caccaacggc gatgagcccg gcgtcctggc gcggcaggtc gccgccgcgg gtcatcggct
     1501 ggtcgtggcg gtcggcggcg acggccaggc ggccgcagtc gcggcgggcc tggtgggcag
     1561 cgacacgacc ttcgccatgc tgccggcggg cagcgccaat gactacgcgc gaacgctcgg
     1621 catgccgcgc ggcaacgtgg ccggcgcggt cgatgcgatc gccgcgggcc ggacggcgca
     1681 cgtcgacacg atcagggtca aggtcgctgg tcgcgagcgg tacttcctga acgtggtcgg
     1741 cacgggcttc gacgccggcg tcgccgcgcg ggccgagagg atcccgctat ttcgtggcgc
     1801 cgggaggtac gtgctcgcgc tgatcgccga gctgccgcgc ttcaaggccg caaccatcac
     1861 cctggacatc gacggcgcga cgcgcgagct gcgcgcgatg atggtcgcga tcgccaacgg
     1921 cgtcagctac ggcggtggca tgcgcgtcgc gccctccgcg acgctcgaca gcggccggct
     1981 cgaagtgtgc gtcgtgggcg agatttccaa accggagttc ctgcgtgcct ttccgcgggt
     2041 gttccggggc acccacgtaa cccatcgcgc ggtgacgatg ctctccgggc gcgaggtacg
     2101 cctggcggcc gaccgtccgc tgcgtctgat cggcgacggc gagtggttcg gcgagctccc
     2161 ggcgacggtg accgttgtac cgcgcagcct gcgggtggtc gtcggaccgg gctaccgcgg
     2221 agccgcggtg agtggttctg agtagtgcgg actagcgcca agcgccccat ccggcgctaa
     2281 aatcgcccgg cgtcgcgcca gcatgtcccg tccgacgttt cgaggagagc cccagacctt
     2341 gtcgcagacc cctgtcctga ccagccccgt cgccgcgcgc gatgtgctga ccgaagagga
     2401 agcgcagcag cgcgccaccc gcgtgagcga caccagctac gacatcgcgc tcgatctcgt
     2461 ggcgggccag tccacctatc gcggcgacgt cacgatccgc tttaacacca gcggcgccgg
     2521 cccgctgttc ctggacttcc gcgggcggga gatcgagctc ctggaggtca atggcctgca
     2581 catcgatccg gactggaccg gctatcggct gaccctgccc gcagaggccg tcagcggctc
     2641 gatggcggtc cacgtcgtct acgtcaacga ttacgacacg accggcgacg gattccaccg
     2701 ctttgtcgat cccgaggacg gcgccgagta cgtctacacc aacttcgagc cgtacgaggc
     2761 ccatcggctc tacccgtgct tcgatcagcc ggacatcaag gcgaggtacg agttcagcgt
     2821 cacggcgccg gccgaatggg cagtcttctc gaatgccccg atcgattcga ccgaggacgc
     2881 cggcgccggc cggacccttc accgcttcgc ccggacggag ctgttcagca gctacctgac
     2941 ggcgctgatc ggcggcgcct acgtgcatcg ctcgacagag cacaacgggg tcagcctgcg
     3001 cctgtacgcg cgcgcttcga tggagcgcta cctcgatgac caggcgccgg agatcttcga
     3061 gatcacgacg cagggcatgg acttctactc cgacttgttc gcccagccct atccgttcgg
     3121 cgtcaagtac gaccaggtgt tcgtgcccga gtacaactcg ggcgcgatgg agaacgtcgc
     3181 ctgcgtcacc tacaacgagg catacctctt ccgcgatccg cccacggaca acgagcgcct
     3241 ggatcgcgcc gagacgttcc tgcatgagct ggcgcacatg tggttcggca acctcgtgac
     3301 gatgcgctgg tggaacgacc tgtggctgaa cgagtccttc gcgacctaca tcagctatct
     3361 ggcgatgact tccgcgaccc gtttccgcaa cgcctggaag gtcttcaact accgcatcaa
     3421 gcgctgggcc taccagaccg accagctgcc gacgacgcac ccgatcgccg gcaccgccgc
     3481 ggacactgag atcgcgttcc tcaacttcga cggcatcacc tacggcaagg gcgcgtccgt
     3541 cctcaagcag ctggccaagt acatcggcct tgactcgttc cgcgacggcc tgcgcctgta
     3601 cttccgccgc cacgcctggg gcaacgcgac cctggcggac ttcctggcct gcctcgagga
     3661 ggcgcacggt gcgtcactcg agaactggtc ggagaagtgg ctgcgcacgg cgtcggtcaa
     3721 caccatcgcc gccaagtggc atgcggctga cggcaagatc gccgatgtgg agatcgagca
     3781 gaccgcgccg gccgaccatc ccacgctacg cccgcacgca ttcgagatcg cgctgatcaa
     3841 tgagggcgcg accgggcttg cggtcgagtc ccagccggcg tggatcgatg ccgcgcagac
     3901 ggacctgccc gagctgcgcg gccacgcggc gccgtcgttg gtcttcccga attacggcga
     3961 ccacgcctac gccaaggtcg cgctcgatga gcggtctctc gatttcgtac gcgccaatct
     4021 cagccgcgtc gacgatgacc tgttgcgcga gctcctgtgg atgtcgctgt gggagatggt
     4081 ccgcgatcgc cagctgcgct cgaccgaata cctcgcgatc gcacgggaac ggatcggctc
     4141 cgagccggac cgcgacatcc tcgacatggc tgtcgagcgc gcggccctgg cgctggcgcg
     4201 cttcgtgccc gaatcacgcc gcacagacga ggcgcacctg tggttcgagg cggcgttgga
     4261 attgcttggc gcagccaagg aggctgacca ccagatcctc tgggcgcgct ccgcggttgc
     4321 cgtcgccgca agcgcggacg atgtggcccg gctcgcaacc gcgaccgcca aagacgagcc
     4381 ggtcaccggc ttcgtactcg accaggagat gcgctggctc gtcgccatca aggcggtggc
     4441 ctttgggctg cctaacgccg acgagttgct ggccatggag gcggctcgtg accgctctga
     4501 tcgcggccgc cgggcgatgc tccgagcgga ggcgtcgcgc ccgaccgaag ccgccaaggc
     4561 ggaggcatgg gagcgcatca acggcgaggg ctacggctcg ttccacctga cgcgcgcggc
     4621 tatgcagggc ttctactggc cgcaccagaa gcggttgctc gagacttacg aggaccgttt
     4681 cttcgaccag gtgcgcacgg tcttcgagac gcgcgaccac ccgttcgcgc gctcgtacct
     4741 gctgtccctg tatccggcat acatgggcga gcccgacgtg ctcgagcggt cgcacgggct
     4801 gctctcgcag ctcaacggct cactgcccac cctgacgcgc cagctgaccg aggctgcgga
     4861 cgacctcgac cgcgtaataa aggtgcgggc attcgccgac aaggggtgag gtaatgaccg
     4921 gccgcgtatg ggtcgagccg gtgacgctcg agggcaccta cgttcgtctc gagccgatgt
     4981 cgcttgacca tctgccgggc ctgacgcagg ttggcctcga tgccgagatc tggcgctgga
     5041 tgccgatgtt cgtccaggcc cccggagaca tgcgcaccct ggttgaagag gccattgccg
     5101 aggcggaggc cgggacgatg gtgccgttca cgaccatcga gcgcgccacc ggtaagccgg
     5161 tgggcagcac gcgctacctg tcgatcgtgc cccagcaccg ccgcctggag atcggctgga
     5221 cctggctcgc gccggcatgg cagcacagtc cggtcaacac tgaggccaag ctgctcatgc
     5281 tgcgccatgc gttcgagaag ctgggtgcgc tacgcgtcga gttcaagacc gacgcgctca
     5341 acgagaagtc gcgcacggcg ctgctgaaga tcggcgcggt cgaggagggc gtgttccgcc
     5401 accacatggt catgcccggc ggccggcagc gcgactccgc gtactacagc gtgatcgaag
     5461 aggaatggcc gcgcgtgcac cagcacctgg aggcacgcct cacgcgcctg gtgcgcgctg
     5521 ccgagcagac cgcgccgcct gtggccgacg cgtcagaggc cgtcgaagca ccggagctga
     5581 tcgaagcgcc ggggtcggac cggggctagg cgaccctcgt gctgccgcgc cggctgggcg
     5641 gtcccggtcg gcggactggg cgcgtcgttg tcgagcggct ggtgcgtccg gaacgggccg
     5701 gccgtgccct gggcgcggcg aggtccggct cgaagccctg gaccacgcgc accggcagat
     5761 ccttcttgag cagcctgttg accgcgcgca ggtaatcctc ttcatcgggc gcgacgagcg
     5821 agatcgcctg gcccgtcgcc ccggcgcggc cggtgcggcc gatgcgatgc acgtagtcgt
     5881 ggggggtatg cggcagctcg tagttgatga cgaggggcag cgcctcgatg tcgaggcccc
     5941 gcgccgcgac atcagttgcg accaaaattg gcacctcgcc cgatttgaag ccggccaggg
     6001 cgcgttcgcg ctcgggctgc gtccggtc
//
LOCUS       DATBRX010000128        12108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3169185, whole genome shotgun sequence.
ACCESSION   DATBRX010000128 DATBRX010000000
VERSION     DATBRX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12108
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3169185"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2075)
                     /locus_tag="VIF08_04580"
     CDS             complement(<1..2075)
                     /locus_tag="VIF08_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8135295.1"
                     /translation="MSLFQRIGAACARQRYRVVAAWGVLLLLALPFAPQLPGALSSGG
                     FTLDDLEASKARQALAGLAVPPSAMVIVIQSETEARAGEPEFEIAVARALAAVPDAEH
                     VTAVLPHTIAPRQVSADGKTVYEVVALDLSPDDSPDALAPVNAAIAPQPGLRIMLAGA
                     PAFYGDIQTLSEDDLRRSEVISLPLAAIALLLVFGSVVAAGVPIVVGGTAVVIALGGL
                     FFVANATPLSIFVLNLATLLGLGLGVDYALLLTSRFREELRARGGGRSSDGTIDQAVV
                     NESVAITVGTAGRAVFFSGLTVLLGLIGLVLFEFMVLRSVGIAGAAVVGLAVSAALTL
                     LPAGLAILGPRIDAFPIRRGATTAGAEGSGAWARLANRVMDRPVLFFGSTLLVLLLLG
                     SPFLHVRFNAPDATILPPGVPSRLAYDVLAREFGEGEFSPLIVAVRTTGPVTDPENVG
                     KLYDYSRTLAADPRVTRVEGMVDVDPRLTRGQYQLIYGAPGGPADRFVRDVLARTSRA
                     DVAAFTVFTPYEGNRREALQLVADMRHPTSSVAPPPGVSVLVGGGAAEVRDTVNRIAA
                     DFPRTAAFILVATYLVLFVLLRSVVLPAKAIIVNALSLTASFGALVWIFQDGNLSALL
                     GFQPLGFVETTLPVILFCVLFGLSMDYEVFLLSRMKEVYDKTGDNRAAVARGMERSGR
                     IVTSAALVVV"
     gene            complement(2081..2614)
                     /locus_tag="VIF08_04585"
     CDS             complement(2081..2614)
                     /locus_tag="VIF08_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8135296.1"
                     /translation="MRTVKVSWDKEAGRFTALGRHPGQQIAINAPAAPSEENRAPTGF
                     SATELLLAGAGSCAAWDVVEILRKRRADVAALDVEVEGQQDADPPWTYRAVALHFRVN
                     GVGLKVPVLARVVRLAIVRYCSVITTLAGVAAISATIELVEPDGSSTGRVRIELALPA
                     VPLPPLLEPTADEDEDE"
     gene            complement(2651..3103)
                     /locus_tag="VIF08_04590"
     CDS             complement(2651..3103)
                     /locus_tag="VIF08_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135297.1"
                     /translation="MPAKPEAHQPMVLSLSAVDRLATMERARQFAIVFSVLLTGAVVV
                     SLIAGFQQLILHPFALIGSTLLAGVVAAIILGVALVPSRRTTSWVSMITGPNGRWFFL
                     ILILLWAAGMAFLASLGMGPQQNGSPAFIGLLAGIFIFMGFIWSVIGE"
     gene            3148..4698
                     /gene="ablA"
                     /locus_tag="VIF08_04595"
     CDS             3148..4698
                     /gene="ablA"
                     /locus_tag="VIF08_04595"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEY8135298.1"
                     /translation="MSSDLTVAPEAFINRRDPALALGPDGRPAVSRRAPKWADVPDEK
                     WNDWRWQLSNRVNDLADVATILNLTEEEREGLGAPDKFRVDITPYFISLIDPDDPNDP
                     IRRQVIPLGREQEAFTAMMEDSLAEDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCT
                     RSRIVGDPTQNFNRAEHEAQLDYLRRTPQVRDVLISGGDGLTLAPKLLENIIRGLREI
                     PHIEIIRIGTRVPVFMPQRVDDELCEMLGKYHPIWMNLHFNHANEITPEVARAVDKLT
                     RAGIPIGNQSVLLAGVNDCVNIQRELVHKLVEMRVRPYYLYQCDLVEGSGHFRTPVGK
                     GLEIIEGLRGHTSGYAVPTYVIDAPGGGGKIPVMPNYLISYSDHKVILRNYEGYITTY
                     EEPLEYKQHDKAACDACQHQRPEPGQSGVLGLLEGERMWIEPRGFEEVHARGNDQPHR
                     LQRPDKWTPYGVGAIEGTSRPTLPVLPADAGAANGNGASQKTDYGPGEEPRPPEGAPT
                     GSSHGELL"
     gene            4839..5606
                     /locus_tag="VIF08_04600"
     CDS             4839..5606
                     /locus_tag="VIF08_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135299.1"
                     /translation="MKRRDWRRAAGITAIPLLLVLSVSSAYAGTSSTGGDGVSLAGMV
                     VPGYPVSAAQTAPGACNDSAYSLLGGKWKQTLNWRYQSSSTPGDMNGSKVLNVIKRSF
                     DNITGARNDCGLADNVSATSHYLGTTSAKPNVNKRGRCSARDDQNVVGFGPLPPGILA
                     VTCIRYGSNNSITEADIRINTEYDWVLTVGACHFWQELLEPTLTHEIGHMFGLGHVGE
                     RKHGRLTMSTTSDGPCSNAETTLGRGDVRGLRHLYPL"
     gene            5606..5974
                     /locus_tag="VIF08_04605"
     CDS             5606..5974
                     /locus_tag="VIF08_04605"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2255 family protein"
                     /protein_id="HEY8135300.1"
                     /translation="MRFDPAAVGLIDAAYEVDIESTRPDGTKRTTTIWAVVDGGEVFV
                     RSWKGDRGYWYQAALDRPDELALIVDGTRLEVRAVLANDEAGIARCSAGLAKKYRRDS
                     SLPGMLLPRVLPTTLRLEPR"
     gene            5971..6384
                     /locus_tag="VIF08_04610"
     CDS             5971..6384
                     /locus_tag="VIF08_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135301.1"
                     /translation="MKLRDGAGLPDPAIAPPVVAESADPFAGLRVAHLIARLPRGERV
                     RLADVVDRLNAEYLDWSFSRNVVAAVAVQLAANWQADFRTTVGFELTAGERGDELLIE
                     DTPRAQTWLIGQVERYAAGCRLRLQAFARHEGDPS"
     gene            complement(6381..7451)
                     /locus_tag="VIF08_04615"
     CDS             complement(6381..7451)
                     /locus_tag="VIF08_04615"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8135302.1"
                     /translation="MVDALRREPARLDEVFAALRGRVVAGDLPAAGLAVGDGEGSIRA
                     EVTGEGGRPISTDTNFFLASITKPLVATAFMELVEDGAVGLHDPVARWVPGMDVGEKA
                     GVTPWHVLTHTSGLVDVPPDELVRQRPSAAKMLRAVVDKPLRFTPGTRWEYNSASFYL
                     LTAIIERVTGMPYVRYLEERLARPLDLGLTFDPRRSRRPVIKVHGAGVDNRFVRWFVV
                     RYLAGAALPGGGLFGNLADLAALGAALVSPRPAGGRAAFPVRAETVAEMAEDQTHGLP
                     GEIDGEERAVHVGLGWAKPTLMRDVPGSKSVISHGGVSGGRIWIDLDIGLVIVFLTNR
                     WQADRGPEIDTIRGVYEALERS"
     gene            7490..7816
                     /locus_tag="VIF08_04620"
     CDS             7490..7816
                     /locus_tag="VIF08_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135303.1"
                     /translation="MKRTELIERTQRLVAEGERLQKQPSMPALRTWLQISDELLSQAW
                     GAMDRYHLAWLGVGRVATIRGRAMTPDEESVYVRDVAEQKTAALKMSLRAVAEQNMPF
                     LGEERG"
     gene            complement(7813..8136)
                     /locus_tag="VIF08_04625"
     CDS             complement(7813..8136)
                     /locus_tag="VIF08_04625"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEY8135304.1"
                     /translation="MPKLLIAVVHEDDAQALVEALRAADLRVTEALSRGGFLKGRNAM
                     LYIGLEDVQVPAAMKLIEENCRSRVQDVPAEPLGAIEASWLPTQVTHGGATVFVVPLE
                     EIRRI"
     gene            complement(8161..8619)
                     /locus_tag="VIF08_04630"
     CDS             complement(8161..8619)
                     /locus_tag="VIF08_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135305.1"
                     /translation="MNDQPTQLQDPTDSPRFALGLGLGWLTAAAFAVIYALSAELVGL
                     TFGLPIIGLAGGWLIGTAVAYGGWGEFEHLPQRGLRLGVVALAVEGWLLGLILAYILS
                     QAFIPQASTPLSDRLSVTGFFDYFLGLDFARFLHLLALPLATFMAWRAAR"
     gene            8641..9144
                     /locus_tag="VIF08_04635"
     CDS             8641..9144
                     /locus_tag="VIF08_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135306.1"
                     /translation="MAHPSLGLPPADARAGLPDAAARLRANSERICRVALQAAERLEP
                     GMGERYDDAMLATFLRDYERHLEQLARAMETGDDSFVVNYGEWVIPIYRRRRVSMRDF
                     MALLGGLRDAAGGVLTPDENRAAAQLFERWSDRLKHHGRLPGDHKGNPIIRFFWKGAG
                     IGDDKWI"
     gene            9158..9469
                     /locus_tag="VIF08_04640"
     CDS             9158..9469
                     /locus_tag="VIF08_04640"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEY8135307.1"
                     /translation="MTIKWVKSDPLVMNGEPFCYATRLTVRNLLEMRANGLTPADMLA
                     NHPELRRVGIAEAFKYAADNRARYAEFFDGSSLRGPGFSADDAAGLPEHLRDMSGVVA
                     G"
     gene            9504..9875
                     /locus_tag="VIF08_04645"
     CDS             9504..9875
                     /locus_tag="VIF08_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEY8135308.1"
                     /translation="MTDQISTDDAPATAATATPATAPTAAPTFDPQARATELAILDAL
                     RAVVDPEIGMNVVELALIRQILLSPEQTEVKMILTTPFCPYAGSMIQQVKEQVESVVD
                     NDVKVTLLAERWDPRDAGLVW"
     gene            9869..10465
                     /locus_tag="VIF08_04650"
     CDS             9869..10465
                     /locus_tag="VIF08_04650"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY8135309.1"
                     /translation="MVARRSAIAKSAVATGKGDDGTTGLLYGGRIAKDDPRTEAYGTI
                     DEAVAALGLARAELNETGLDELAESVLRWQRELFVVGADLATAPDKRGRLEDGVTRVS
                     EVMLDGVEVELALWESAIEMPREFIVPGETRASAALEVARVILRRAERRVIAADHANP
                     LGSDWIVPYINRLADLLWVLARAAEQAAGAPATTARVR"
     gene            10462..10875
                     /locus_tag="VIF08_04655"
     CDS             10462..10875
                     /locus_tag="VIF08_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135310.1"
                     /translation="MSRGFRLLLGLVLGLSVLVGCSEIGINLDTPPPPSGIKGTVLLG
                     PTCPVESSPGANDPVPCLTPYAATLVVLDDEGVKVATVTSGADGKFQLDLAPGDYVVT
                     PESGTDTYPIAQPQSVTVATGAYTEIQVNYDTGIR"
     gene            complement(10897..11490)
                     /locus_tag="VIF08_04660"
     CDS             complement(10897..11490)
                     /locus_tag="VIF08_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY8135311.1"
                     /translation="MDFADVVRKRRMVRNYDASRPVARDVLERIAAAAQRAPSAGFSQ
                     GQRIVIVTDPARRARIAEICDEAEYVAAGFDPWVSRAPALFIPTVSEQIYHRRYQEPD
                     KVNDDGTEIDWPVPYWWTDIGATALLIHQAAIAEDLAAGFLGLADYTDLKAYLGIPDE
                     HAPIGIITVGYALPDRRSQSLKRGWVAMDEFAHWEKW"
     gene            complement(11490..11906)
                     /locus_tag="VIF08_04665"
     CDS             complement(11490..11906)
                     /locus_tag="VIF08_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8135312.1"
                     /translation="MSSQPRPLVLGIFRRGPLMLVGEGYDRVRDQRFYRPLGGQIEPG
                     ESPEQALVREMREELGDEIRSIRHLGELDNRFVFEGRPGWEQVHVLEAAFVSSAAPHV
                     NSVEAEGWNLKWIDPNALDLPLYPDGLADLLRLASS"
BASE COUNT         2070 a         4159 c         4125 g         1754 t
ORIGIN      
        1 acgacgacca gtgccgccga cgtgacgatc cggccgctgc gctccatgcc gcgcgcgacc
       61 gccgcgcggt tgtcgccggt cttgtcgtag acctccttca tgcgcgacag caggaatacc
      121 tcgtaatcca tcgataggcc gaagaggacg cagaacagga tgacgggcag cgtcgtctcg
      181 acaaagccga gcggttggaa gccgagcagc gccgagaggt tgccgtcctg gaagatccag
      241 acgagggcgc cgaaggaggc ggtcagcgag agcgcgttga cgatgatcgc cttcgccggg
      301 agcacgaccg agcgcagcag cacgaacagc accaggtacg tcgcgacgag gatgaacgcg
      361 gccgtacgtg ggaagtcggc cgcgatccgg ttgacggtgt cgcgtacctc tgccgcgcca
      421 ccaccgacca gcacactgac gccgggcggt ggcgcaaccg aggacgtcgg gtggcgcatg
      481 tcggcgacga gctgcagcgc ctcacgccgg ttgccctcgt acggtgtgaa caccgtgaac
      541 gcggcgacgt cagcgcggct cgtccgcgcc aggacgtctc tcacgaaacg atccgccgga
      601 ccaccggggg cgccgtagat cagctggtat tggccgcgcg tcaggcgcgg gtcgacgtcg
      661 accatgccct cgacgcgggt tacgcgcggg tctgcggcca gtgtgcgcga gtagtcgtac
      721 agcttgccga cgttctccgg atcagtgacc gggccggtgg ttctgaccgc gacgatcagc
      781 ggcgaaaact caccctcgcc gaattcgcgc gcgagaacgt cgtaggccag ccgtgatggc
      841 acgccgggcg gcaggatcgt cgcgtccggc gcgttgaaac gcacgtgtag gaatggcgag
      901 ccgagcagca gcagcacgag cagcgtgctg ccaaagaaca ggaccggtcg gtccatcacg
      961 cggttggcca ggcgcgccca cgcgccactg ccctcggcgc ccgctgtggt ggccccgcgc
     1021 cggatgggaa acgcgtcgat gcgcgggccg aggattgcga gtccggccgg cagcagggtc
     1081 agcgccgcgc tgaccgccag gcccacgact gccgctccgg cgatgccgac cgagcgcagg
     1141 accatgaact cgaagagcac gaggccgatc aaccccagga gcacggtcag gccgctgaag
     1201 aagacagccc gcccggcggt cccgacggtt atcgccaccg actcgttgac gacggcctgg
     1261 tcgatcgtgc cgtcactgga gcggccgccg ccgcgggcgc gcagctcctc gcggaagcgg
     1321 ctcgtcagga gcagcgcgta gtcgacgccc agccccaggc cgagcagcgt cgccaagttg
     1381 agcacgaaga tcgagagtgg cgtggcattg gccacgaaga acaggccacc gagtgcgata
     1441 acgacggccg tgccaccgac gacgatgggc acgccggccg cgacgacgct gccgaagacg
     1501 agcaggagcg caatcgcggc gagcggcagc gagatgacct cgctccgccg caggtcgtcc
     1561 tccgatagcg tctggatgtc cccgtagaac gcgggcgcgc cggcgagcat gatgcgcagc
     1621 cccggttggg gcgcgatcgc ggcattgacc ggagcgagcg cgtcgggcga atcgtcgggc
     1681 gagaggtcga gcgccaccac ctcgtacacc gtcttgccgt cagcgctgac ctggcgcggc
     1741 gcgatggtat gcggcagcac ggcggtgaca tgctctgcgt ccggaacggc cgcgagcgcg
     1801 cgggccaccg cgatctcgaa ttcgggctcg cccgcgcgcg cctccgtctc gctctggatg
     1861 acgatgacca tcgcgctcgg tggcaccgcg aggcccgcga gcgcctggcg cgccttggac
     1921 gcctccaggt catcgagagt gaatccgccg gagctcaggg cacccggcag ctgcggcgcg
     1981 aacggcagcg ccagcagcag cagcacgccc caagcggcaa cgacgcggta ccgctggcgg
     2041 gcacaagccg cgcctatgcg ctgaaagagg ctcaccccgc ctactcgtcc tcgtcttcat
     2101 cggcagttgg ttcgagaagc ggcggcagcg gcacggccgg cagcgccagc tcgatccgga
     2161 cgcgtcccgt gctcgagcca tccggctcaa ccagctcgat cgtggccgag atggccgcga
     2221 cgcccgcgag cgtcgtgatg accgagcagt agcgcacgat ggccaggcgc acgacgcgcg
     2281 ccagcacagg caccttcagg cccacgccgt tgacgcggaa gtgcagcgct acggcgcggt
     2341 atgtccaggg tgggtccgca tcctgctggc cctcgacctc gacatcgagc gcggccacgt
     2401 ccgcgcgccg cttgcgcagg atctcgacca cgtcccacgc cgcgcacgac ccggcgccgg
     2461 cgagcagcag ctcggtggcg gagaacccag taggggcgcg gttctcctcg gacggcgcgg
     2521 ccggagcgtt gatcgcaatc tgctggccgg ggtgtcgacc gagcgcggtg aatcggccgg
     2581 cttctttgtc ccaggagact ttgacggttc tcatgcgcgc gggcgggcgg cgccgcctgg
     2641 ggtcaggcgg ctactcgccg atgaccgacc agatgaagcc catgaagatg aagatgccgg
     2701 cgagcagccc aatgaacgcg ggtgagccgt tctgctgcgg acccatgccc aggctcgcca
     2761 ggaaggccat gcccgcggcc cagagcagga tcaggatcag gaagaaccag cggccgttcg
     2821 gcccggtgat catcgacacc cagctggtag tgcggcgcga cgggacgagc gcgacaccca
     2881 ggatgatcgc ggccacgact ccggccagca aagtcgagcc gatcagggcg aagggatgca
     2941 ggatcagctg ctggaaaccg gcgatgagcg acaccacgac cgccccggtg agcagcacgg
     3001 agaaaacgat cgcgaattgc ctggctcgct ccatcgtcgc gagtctatca actgcgctga
     3061 gcgacaggac cattggctgg tgggcctccg gcttggccgg cacgctatca tgccccacta
     3121 tccactctgc ataaggaata tgcactggtg agctcagatc tgaccgtcgc gcctgaggcg
     3181 ttcatcaacc gtcgcgatcc cgcgctggcg ctcggccccg atggccggcc cgcggtgagc
     3241 cgccgcgcgc ccaagtgggc ggacgttccc gacgagaagt ggaacgactg gcgctggcag
     3301 ctctcgaacc gcgtcaacga cctggcggac gtcgcgacca tcctcaacct gacagaggaa
     3361 gagcgcgagg gcctgggcgc gcccgacaag ttccgggtgg acatcacgcc ctacttcatc
     3421 tcgctcatcg accccgacga tcccaacgac cccatccgcc gccaggtcat cccgctgggc
     3481 cgcgagcagg aggccttcac ggcgatgatg gaggattcgc tcgccgagga ccgccactcc
     3541 cctgtaccgg gactggtcca ccgctatccc gaccgggtgc tgatgctggt cacgacgcag
     3601 tgcgcgtcgt actgccgcta ctgcacgcgg tcgcgcatcg tgggcgaccc cacgcagaac
     3661 ttcaatcgcg ccgagcacga ggcgcagctc gactacttgc gccgcacgcc ccaggttcgc
     3721 gacgtgctaa tcagcggtgg cgacggcctc acgctcgcgc ccaagctgct cgagaacatc
     3781 atccgcggcc tgcgcgagat cccgcacatc gagatcatcc gcatcggcac ccgcgtgccg
     3841 gtcttcatgc cgcagcgcgt cgatgacgag ctgtgcgaga tgctcggcaa gtaccacccg
     3901 atctggatga acctgcactt caatcacgcc aacgagatca cgcccgaggt ggcgcgcgcg
     3961 gtcgacaagc tgacccgcgc cggcatcccg atcggcaacc agagcgtgct ccttgccggc
     4021 gtcaacgact gcgtcaacat ccagcgcgag ctcgttcaca agctcgtgga gatgcgcgtc
     4081 cgcccgtatt acctctacca gtgcgacctg gtcgaaggct cgggccactt ccgaaccccg
     4141 gtcggcaagg gtctcgagat catcgagggc ctgcgcggcc acacctccgg ctatgccgtg
     4201 ccgacgtacg tcatcgacgc ccctggcggt ggcggcaaga tcccggtcat gcccaactac
     4261 ctgatcagct actccgacca caaggtgatc ctgcgcaact acgagggcta catcacgacc
     4321 tacgaggagc cgctcgagta caagcagcac gacaaggcgg cgtgcgacgc gtgccagcac
     4381 cagcggccgg aaccgggtca gtcgggggtg ctcggcctgc tcgagggtga gcggatgtgg
     4441 atcgagccgc gcggctttga ggaagttcac gcccgcggca acgaccagcc ccatcgcctg
     4501 cagcggccgg ataagtggac gccctacggc gtcggcgcga tcgagggcac ctcgcgcccg
     4561 acgctgccgg tgctgcccgc ggatgctggc gcggccaatg gcaacggcgc ctcacagaag
     4621 accgactacg ggccgggcga ggagccccgg ccacccgagg gcgcacccac cggctcgtct
     4681 cacggggagt tgctgtagca agtgcagcgc atgaacgcgc gctagcgcgg ccttgcgcgg
     4741 agcgcgcagc agcggcccga aggccgactg cggagatgta gtaggtagga cgatcgcgtc
     4801 agatggggga gggcgcgaaa ttaggtaagg tgtcaccaat gaaacgccgc gactggcgcc
     4861 gcgcggcggg gatcacggca atcccgctac tgctggtgct ttctgtcagc tctgcctacg
     4921 cgggcacctc aagcacgggc ggtgacggcg tgtcgcttgc cggcatggtc gtgcccggct
     4981 acccggtgtc cgcggcgcag actgcgccag gtgcctgcaa cgactctgcc tattcgcttc
     5041 tcgggggcaa gtggaagcag acgctcaatt ggcgctatca gtcgtcatcg accccgggcg
     5101 acatgaacgg atccaaggtc ctgaacgtga tcaagcgctc gttcgacaac atcaccgggg
     5161 cgcgcaacga ctgcggcctt gccgacaacg tcagcgccac ttcgcactac ctcggcacga
     5221 catcggccaa gccgaacgtc aacaagcgcg gccggtgcag tgcgcgcgac gaccagaatg
     5281 ttgtcggctt cgggccgctg ccgccgggca tcctcgcggt cacgtgcatc cgctacggca
     5341 gcaacaacag catcactgag gcggatatcc gcatcaacac ggaatacgac tgggtattga
     5401 ccgtgggggc gtgccacttc tggcaggagc tgctcgaacc cacgctgacc cacgagattg
     5461 gccacatgtt cggactgggc cacgtcggcg agaggaagca cggccggctg acgatgagca
     5521 ccacctctga cgggccgtgc agcaacgccg agacgactct cggcaggggc gacgtgcggg
     5581 ggctgcgcca cctctacccg ctgtagtgcg cttcgacccg gcggcggtcg gtctgatcga
     5641 cgcggcctac gaggtcgata tcgagtcgac ccgacctgac ggaaccaagc gaaccaccac
     5701 catctgggcc gtagtcgacg gtggcgaggt gttcgtgcgc tcgtggaagg gtgatcgcgg
     5761 ctactggtac caggccgccc tggaccgacc cgatgagctc gcgctgatcg ttgatggcac
     5821 acgcctggag gtacgggccg tgcttgccaa tgacgaggcc ggcatcgcgc gctgctcggc
     5881 cggactggcg aagaagtatc gccgcgactc gtcgctcccc gggatgttgc tgccgcgcgt
     5941 cctaccgacc accctgcgac tcgagccgcg atgaagctgc gcgatggcgc gggtctgccg
     6001 gatcccgcga ttgcgccacc ggtggtcgct gagtcggccg atcctttcgc cggcctgcgc
     6061 gtcgcccatc tgatcgcgcg gctaccgcgc ggcgagcgcg tccggctggc tgacgtcgtc
     6121 gatcgcctga acgcggagta tctcgattgg tcgttctcgc gcaacgtggt cgccgcggtg
     6181 gcggtccagc tcgccgcgaa ctggcaggcc gatttcagga cgacggttgg cttcgagctg
     6241 accgccggcg agcgtggcga cgagctgctg atcgaggaca ccccccgggc gcagacgtgg
     6301 ctgatcggcc aggtcgagcg ctacgcggct gggtgccggc tgcgcctgca ggccttcgcg
     6361 cgccacgagg gcgatccctc ctaggagcgc tccagcgcct cgtacacccc acggatcgtg
     6421 tcgatctccg gcccgcgatc ggcctgccag cggttggtca ggaagacgat caccaggccg
     6481 atgtccaggt cgatccagat ccgcccgccg ctcaccccgc cgtggctgat cacgctcttg
     6541 gagcccggca cgtcgcgcat caaggttggc ttggcccagc ccaggccgac gtgcacggcg
     6601 cgctcctcac cgtcgatctc gccgggcagg ccgtgcgtct ggtcttcggc catctcggcg
     6661 acggtttcgg cgcgcaccgg gaaggccgcg cgtccccctg ctggtcgcgg cgaaaccagg
     6721 gccgcgccca gtgccgcgag gtccgcgaga ttgccgaaca atccgccgcc cggcagcgcc
     6781 gcgccggcca ggtagcggac cacgaaccag cgcacgaagc ggttgtcgac gcccgcgccg
     6841 tgaaccttga tgaccggccg gcgcgaccgg cgcgggtcga aggtcagccc caggtcgagc
     6901 ggtcgcgcca ggcgctcttc gaggtagcgg acgtagggca tcccagtgac gcgctcgatg
     6961 atcgcggtga gcaggtagaa cgacgcgctg ttgtattccc agcgcgtgcc cggagtgaag
     7021 cgcagcggct tatcgacgac cgcgcgcagc atctttgctg ccgacgggcg ttggcgcacc
     7081 agctcgtcgg gtggcacgtc taccagcccg gacgtgtgcg tcaagacatg ccacggcgtc
     7141 actccggcct tctcgccaac atccatgccg ggcacccagc gcgcgaccgg atcgtgcaga
     7201 cccacggcgc cgtcctcgac gagctccatg aaggccgtgg cgacgagggg cttggtgatc
     7261 gatgccagga agaagttcgt atcggtgctg atcggccgtc cgccttcgcc cgtcacttcg
     7321 gcacggatcg agccttcgcc gtcgcccacc gcgaggccgg ctgccggcag gtcaccggcc
     7381 acgacacggc cgcgaagtgc cgcgaacacc tcatcgagcc gcgcgggctc gcgccgcaga
     7441 gcatcaacca cgccccgacc atagcgccgc gatgggctac ggtcctgcgg tgaagcggac
     7501 tgagctcatc gagcgcaccc aacgactcgt cgctgagggc gagcggctcc agaagcagcc
     7561 gagcatgccc gcgctgcgca cgtggctgca gatatccgac gagctgctga gccaggcgtg
     7621 gggcgcgatg gaccgctacc acctcgcgtg gctgggcgtc gggcgcgtcg cgacgatccg
     7681 cggccgcgcc atgacgcccg acgaagagtc cgtttacgtg cgcgacgtcg ccgagcagaa
     7741 gacagcggca ctgaagatga gcctgcgcgc ggtggcggag cagaacatgc ccttcctcgg
     7801 cgaagagcgc ggctagatcc ggcggatctc ctcgagtggg acgacgaaca cggtggcgcc
     7861 accgtgggtg acctgtgtcg gcagccacga cgcctcgatc gcgccgagtg gctctgccgg
     7921 cacatcctgg acacgggagc ggcagttctc ctcgatcagc ttcatggccg cgggcacctg
     7981 tacgtcctca aggccgatgt acagcatcgc gttgcggccc ttcaggaagc cgccgcgtga
     8041 cagcgcctcc gtcacgcgca ggtcggcggc gcgcagcgcc tcgacgagag cctgggcgtc
     8101 gtcctcgtgt acgaccgcga tcagcagctt tggcacgcgc ccatcatcgg gcagtagccg
     8161 ttatcgcgcc gcgcgccacg ccatgaacgt ggccagcggc agcgccagca ggtgcaggaa
     8221 gcgcgcgaag tcgagcccga ggaagtagtc gaaaaagccg gtcacggaca gtcggtcgct
     8281 gagcggcgtg gaggcctgcg gtatgaatgc ctggctcagg atgtaggcaa ggatcagccc
     8341 gagcagccag ccctcgaccg cgagcgccac cacgcccagc cgcaggccgc gctgcggcag
     8401 atgctcgaac tcaccccagc cgccataggc gaccgcggtg ccgatgagcc agccaccggc
     8461 cagcccgatg atcggcagtc cgaaggtcaa cccgaccagc tcggccgaca gcgcgtagat
     8521 gaccgcgaac gcggccgcgg tgagccagcc caggcccagg cccagggcaa agcgcggcga
     8581 gtcggtggga tcctgaagct gagtcggctg gtcattcaca cccgatagac taggtcgccg
     8641 atggcccacc caagtctcgg actgcccccc gcggatgccc gcgccggcct gccggacgcc
     8701 gcggcccgcc tgcgcgcgaa tagcgaacgc atctgccgcg tcgccctcca ggccgccgag
     8761 cgcctcgagc cgggaatggg cgagcgctac gacgacgcca tgctggccac cttcctgcgc
     8821 gactacgagc gccatctcga gcagctcgcc cgggcaatgg aaacgggcga cgacagcttc
     8881 gtcgtgaact acggcgagtg ggtgatcccc atctaccgca ggcgccgcgt atcgatgcgc
     8941 gacttcatgg cgctgctggg tggcctgcgc gacgccgcgg gcggagtgct gacgcccgac
     9001 gagaatcgtg ccgcagcgca actgttcgag cgctggagcg accgactgaa gcatcacggc
     9061 cggttgccgg gcgaccacaa gggcaacccg atcatccgct tcttctggaa gggcgccggc
     9121 atcggcgacg acaagtggat ctgacggggc cgattcgatg accatcaaat gggtcaagag
     9181 cgacccgctc gttatgaacg gcgagccgtt ctgctacgcc acacgcctga cagtgcgcaa
     9241 cctgctcgag atgcgcgcca atggcctcac gcccgcggac atgttggcta accaccctga
     9301 gctgcgccgc gtcggcatcg cggaggcatt caagtatgcg gcggacaaca gggcgcgcta
     9361 tgcggagttc ttcgacggat catcgctgcg cggcccgggt ttcagcgccg acgacgcggc
     9421 cggactgccg gagcacctgc gcgacatgag cggggtcgtc gccggctaac aacgggtgac
     9481 cggtgctacg atctatatcc gacatgaccg atcagatttc caccgacgac gcgcccgcga
     9541 ccgccgcgac cgccacaccc gccacggcac cgacagctgc gcccaccttc gacccgcagg
     9601 ctcgtgccac tgagctcgcc atccttgatg cgctgcgcgc cgtggtcgat cccgagatcg
     9661 gcatgaacgt ggtcgagctc gcgctgatca ggcagatcct gctcagcccc gagcagaccg
     9721 aagtcaagat gatcctgacc acgcctttct gcccttatgc tggttcgatg atccagcagg
     9781 tcaaggagca ggtcgagagc gtcgtcgaca acgacgtcaa ggtgaccttg ctggccgagc
     9841 gctgggatcc gcgcgacgcc ggactggttt ggtagcgcgc cgctccgcaa ttgcgaagag
     9901 cgccgtcgcc accggcaagg gcgatgacgg cacgaccggc ctgctgtacg gcggccggat
     9961 agccaaggac gacccacgga ctgaggccta cggcacgatc gatgaagccg tcgcggccct
    10021 gggcctggcg cgcgccgagc tgaatgaaac cggtctcgat gagctcgccg agtcggtact
    10081 ccgctggcag cgcgagctgt tcgtggtcgg tgccgatctg gccacggccc ccgacaagcg
    10141 cgggcgactc gaggatggcg tgacgcgcgt ttccgaagtg atgctcgacg gcgtcgaggt
    10201 tgagctggcg ctctgggagt cagcgatcga gatgccgcgc gagttcatcg ttcccggcga
    10261 gacgcgggcg tcggccgcgc tcgaggtggc gcgcgtcatc ctgcgccgcg ccgaacggcg
    10321 tgtcatcgcc gctgaccacg ccaaccccct ggggagcgac tggatcgtgc cctacatcaa
    10381 tcgcctcgcc gatctgctgt gggtgctggc gcgcgccgcc gagcaggccg cgggcgcccc
    10441 tgctacaaca gcccgcgtca gatgagccgt ggctttcgcc tcctgcttgg tctcgtgctg
    10501 ggcctgtcgg tgttggtcgg ctgctcggag atcggcatca acctcgacac cccgccgccg
    10561 ccgtcgggca tcaagggaac ggtgctgctc gggccaacct gtccggtcga gtccagcccc
    10621 ggcgccaacg acccggtgcc gtgcctgact ccctatgccg ccaccctggt cgtgctcgac
    10681 gacgagggag tcaaggtggc caccgtcacc tccggcgcgg acggcaagtt ccagcttgac
    10741 ctcgcgccgg gtgactacgt ggtgacgcct gagagcggca ccgacacgta ccccattgcc
    10801 cagccccagt cggtcacggt ggcgaccggc gcgtacacgg agatccaggt caactacgac
    10861 accggcatcc gctaggccgg tcgggcgcaa gctgcgctac cacttctccc agtgggcgaa
    10921 ctcgtccatg gcgacccagc cgcgtttgag tgattgggag cggcgatcgg gcagcgcgta
    10981 gccgacggtg atgattccga tgggtgcgtg ctcgtcgggg atgcccaggt aggccttcag
    11041 atcggtgtaa tcggccaagc cgaggaagcc cgcggcgagg tcttcggcaa tcgccgcctg
    11101 gtggatgagc agcgccgtcg cgccgatgtc ggtccaccaa tacggcaccg gccagtcgat
    11161 ctccgtgccg tcgtcgttga ccttgtccgg ttcctggtag cggcggtggt agatctgctc
    11221 gctcaccgtg ggaatgaaga gtgccggcgc gcggctgacc caaggatcga agcctgcggc
    11281 gacgtactcg gcttcgtcgc agatctcggc gatgcgggcg cgccgcgccg ggtccgtaac
    11341 gatgacgatg cgctggccct gcgagaagcc ggcgctcggc gcgcgctgag cggcggcggc
    11401 gatgcgctcc aggacgtccc gcgccacagg ccgtgacgcg tcgtagttgc gcaccatgcg
    11461 ccgcttgcgg acgacgtcgg caaagtccat caggaggagg ctaggcgcag caggtcggcg
    11521 aggccatccg gatagagcgg gaggtcgagc gcgttcggat cgatccactt caggttccag
    11581 ccctccgcct cgacgctgtt cacgtgtggt gccgcggaag agacgaacgc cgcctccagc
    11641 acatgcacct gctcccagcc cggccggccc tcgaacacga atcggttgtc gagctcaccc
    11701 aagtgacgaa tggagcggat ctcgtcgccg agctcttcgc gcatctcgcg caccagcgcc
    11761 tgctcagggc tctcaccagg ctcgatctgg ccaccgagcg gccggtagaa ccgctgatcc
    11821 cggacgcggt cgtagccctc gccgacgagc atgagggggc cgcgtcggaa gatgcccagc
    11881 acgagcgggc gtggctggct gctcatttcg ctcggattgt cgtcgagtca cgctggcgcg
    11941 gctggccaga acgggttgtt gcccctacct tggcaagtta gccagggagg gaacgtgcgg
    12001 caggagcgaa aacccatgaa gatccggcgt cgtcctcact tgcagcgatt agcgccggtc
    12061 ctatgggtct cgactctggc gctgctctcg gcctgtggat catgggca
//
LOCUS       DATBRX010000129         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2833638, whole genome shotgun sequence.
ACCESSION   DATBRX010000129 DATBRX010000000
VERSION     DATBRX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2833638"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VIF08_04670"
     CDS             complement(<1..387)
                     /locus_tag="VIF08_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135313.1"
                     /translation="MKEDDVRLGRVIRLIRHQACTTQAQLASSANVPLADLKLVEAGR
                     ASAVKLGRIRAILEALDGRAKLVPWWKGAAADRLLDERHAAIAERCAGYFPRPSWATH
                     LEVSFAEYGERGSIDILGANRAALAVA"
     gene            complement(448..1155)
                     /locus_tag="VIF08_04675"
     CDS             complement(448..1155)
                     /locus_tag="VIF08_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY8135314.1"
                     /translation="MTVAAVILFARPEGALADAAGRVAARRMVESAWAGGATPIVVVA
                     SDPTGALAAALAGSPAVLAEPAPVEMGPVGQIARGIDVAAQLVTETDAALVWPGRLAW
                     VDAETVGSLIEGHGPRRESILRPLYEGEAGWPVLVPMTVLPLVRSQAPTLMPDELIAA
                     TVEAGVRIELIDTGDPGVTHDISTPLQSLPPYQGPPEPAGGASPEWGAAAADMPDDAP
                     LEGPALAPYSQAADEGA"
     gene            1206..3137
                     /gene="typA"
                     /locus_tag="VIF08_04680"
     CDS             1206..3137
                     /gene="typA"
                     /locus_tag="VIF08_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY8135315.1"
                     /translation="MSATASVSVRNDLRNLAIVAHVDHGKTTLVDAMLRQSGVFHEYE
                     HLVERVMDSMDLEREKGITILAKQTTVEYHPTNDPTHVIRLNIVDTPGHADFGGEVER
                     TLLMVDAILLLVDAAEGPLPQTRYVLSKALARSLPTVVAINKIDRQDARPKEVLDAIY
                     ELFIDLGADANQLDFPILYTNAKLGTATPDLQKPGTDLRPLFDALVSTTPPPTYEPDH
                     PLQLLVTNLSANDYVGRMAVGRIWNGSIKKGQRISVVRAEPEAPDGTVDPDRTVTLTG
                     TVTSLTTAQGIERVDIDQAGPGEIVAVAGIPEVTIGDTITDPADPRPLPRLEVDQPTL
                     SMTFGVNTSPLSGRDGKFVTSRQIKDRLEREVLGNVSIEVLPTASPDTYEVRGRGELQ
                     LAVLIEQMRREGFELQVSRPEVLLREVNGVTQEPYEQITVDIPPEFIGPVSQSLAARR
                     AQLQLMTTDADGRTRMEFLIPTRGLVGYRGQLLTETRGTALLHQIGAGYGPWAGEVSH
                     RTSGVLVSDRTGESNAYGLFNLQERAQLLIDSGVEVYEGMIIGENSRPTDMDVNPTKE
                     KKLTNIRTHSHDEMLRLTPPPPLTLESAIEFIATDELVEVTPKNLRLRKRLLSQHDRR
                     REAGRAYDERRREELTAAD"
     gene            complement(3445..3855)
                     /locus_tag="VIF08_04685"
     CDS             complement(3445..3855)
                     /locus_tag="VIF08_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8135316.1"
                     /translation="MDTDQMTDYHELLATATSFLLIDWPSRDVPDTLARHGYRVASAD
                     GPRDDEYNAYELADGEVRVRPLDAAPHMIDIVYAHRPLAELERIVEQARALNARAVWL
                     QSGSPQAREIVERAGLMYFDSPYLPDAIRGTAGL"
     gene            3878..>4417
                     /locus_tag="VIF08_04690"
     CDS             3878..>4417
                     /locus_tag="VIF08_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8135317.1"
                     /translation="MTVYLDHAATTPLRREVLDAMMPLLTSDFGNPSSVHGHGRKARA
                     ALDETHERLAHGLNAQPREIIFTGGGSEAINLALKGVAWAGKGRGNRLITSAVEHHAV
                     LHSLRHLEKFGFEIIVLPVDRYGRLDPEQLDNAINDRTILVSLMLANNEVGTIQPLAD
                     LVRRVRAHRGVAIHVDGVQA"
BASE COUNT          743 a         1520 c         1496 g          658 t
ORIGIN      
        1 ggccacggcc agggcagcgc ggttcgcgcc aagaatgtcg atggaccctc tctcgccgta
       61 ttctgcgaac gacacctcca ggtgggtcgc ccacgatggt cttggaaagt aaccggcgca
      121 gcgctcagcg atggccgcgt ggcgctcgtc cagcaggcga tccgcggccg cgcccttcca
      181 ccacggcacg agcttggcgc gtccgtccag cgcctccagg atggcgcgaa ttcgccccag
      241 cttgacagcc gaggcacgtc cggcctccac cagcttcagg tcggccagcg gcacgttcgc
      301 ggaggaagcc agctgggcct gagtcgtgca ggcctggtgg cggatcagtc ggatgacgcg
      361 gccaagtcgg acatcatcct ccttcacgtt gaggagtctg cggagccggc cttatcggag
      421 ccttatgttg tgcttatctc ggccgggcta ggcgccttcg tcggcggctt gggagtaggg
      481 cgccagggcg gggccttcga gcggggcgtc gtcgggcatg tccgcggcgg ccgctcccca
      541 ttctggtgac gctccaccgg cgggctccgg cgggccttgg tatggcggca gcgattggag
      601 cggcgtcgag atgtcgtggg ttacgcccgg atcgccggtg tcgatgagct ctatccgcac
      661 gccggcctcg acggtcgcag cgatgagctc gtcgggcatg agcgtcggcg cctgcgaccg
      721 gaccagcggc agcaccgtca tcggcacgag caccggccag ccagcctcgc cctcgtagag
      781 cggccgcagg atcgactcac gccgcgggcc gtggccttcg atgagcgacc ccaccgtttc
      841 ggcatcgacc caggcgaggc gccccggcca caccagggcg gcgtcggtct ccgtcaccag
      901 ctgcgccgcg acgtcgatgc cgcgcgcgat ctggccgact ggacccatct cgactggcgc
      961 gggctcggca aggaccgcgg gcgatcccgc caaagcagcg gccagcgcac ccgttggatc
     1021 cgacgcgaca accacgatgg gcgtcgcgcc gccggcccat gcgctctcga ccatccgccg
     1081 cgcggcgacc cggccggcgg catcggcgag cgccccctcc ggacgcgcaa aaaggatcac
     1141 ggcagcaacg gtcacaggcg cgatcgtagt ggctgagcca cttcgtggga caatggacct
     1201 tccctatgag cgccaccgct tctgtttccg tgcgcaatga cctgcgcaac ctcgccatcg
     1261 tcgcccatgt cgaccacggc aagaccacgc tcgtcgacgc gatgctgcgg cagagcggcg
     1321 tcttccatga gtacgagcac ctggtcgagc gcgtcatgga ctcgatggat ctcgagcgcg
     1381 agaagggcat cacgatcctc gccaagcaga ccacggtcga gtaccacccc accaatgacc
     1441 cgacgcacgt catccgcctg aacatcgtcg acacgccggg ccacgccgac ttcggtggcg
     1501 aggtcgagcg gacgctgctc atggtcgacg cgatcctgct cctcgtcgac gcggcggaag
     1561 ggccgctgcc ccagacgcgc tacgtgctct ccaaggcgct cgcgcgcagc ctgcccacgg
     1621 tcgtggcaat caacaagatc gatcgccagg acgcgcggcc caaggaagtc ctcgacgcga
     1681 tctacgagct gttcattgac ctgggcgccg acgcgaacca gctcgacttc ccaatcctgt
     1741 acacgaacgc caagctgggc acggcgaccc ctgacctaca gaagccgggc accgatctcc
     1801 ggccgctctt cgacgcactg gtttcgacca ccccgccacc gacctacgag cccgaccacc
     1861 cgctgcagct gctggtcacc aacctatcgg ccaacgacta cgtgggccgg atggccgtcg
     1921 gccgcatctg gaacgggtca atcaagaagg gccagcgcat ttccgtcgtg cgcgcggagc
     1981 cggaggcgcc cgacgggacg gtcgatcccg atcggaccgt caccttgacg ggcacggtca
     2041 cgagcctgac gacggcccag ggcattgagc gagtcgacat cgaccaggcg gggccgggcg
     2101 agatcgttgc cgtcgcgggc attccggagg tgacgatcgg tgacaccatc actgacccgg
     2161 cggacccgcg tccgctaccg cgcctggagg tcgatcagcc cacgctgagc atgaccttcg
     2221 gcgttaacac ctcaccgctg tcgggccgcg acggcaagtt cgtgacgagc cgccagatca
     2281 aggaccgcct cgaacgcgag gtgctgggca acgtctcgat cgaggtgctg ccgaccgcgt
     2341 cacccgacac gtacgaggtg cgcggccgcg gtgagttgca gctcgccgtg ctgatcgagc
     2401 agatgcgccg cgagggcttc gagctgcagg tgtcgcgccc ggaggtgctg ctgcgcgagg
     2461 tgaacggcgt tacccaagag ccgtacgagc agatcacggt cgacataccg cccgagttca
     2521 tcggcccggt cagccagtcg cttgccgcgc ggcgcgccca gctgcagctg atgacgaccg
     2581 acgccgacgg ccggacgcgg atggagttcc tgatcccgac gcgcggcctg gtcggctatc
     2641 gcggccagct gctcacggag acgcgcggca cggcgctgct tcatcagatc ggcgccggct
     2701 acgggccctg ggcgggggag gtgtcgcacc gaacgtcggg agtgctggtg agcgatcgga
     2761 ccggagaatc gaacgcatac gggctgttca acctgcagga gcgcgcgcag ctgctgatcg
     2821 actcaggcgt cgaggtctac gagggcatga tcattggcga gaactcccga ccgactgaca
     2881 tggatgtcaa cccaaccaaa gagaagaagc tgaccaacat ccgcacccac tcgcacgacg
     2941 agatgctgcg cctgacgccg ccgccgccgc tgaccctgga gagcgcgatc gagttcatcg
     3001 cgaccgacga gctggtcgag gtgacgccca agaatctccg gttgcgcaag cggctgctga
     3061 gtcagcatga ccggcggcgc gaggccggcc gcgcctatga cgagcgccgg cgcgaggagc
     3121 tgaccgccgc cgattgaatg gcccccgcgg gggttcccag cggtgagaga atcgggacag
     3181 gaggcgcaag gtgcaggtcg tgctcctgtt gttggcagtg gtccttgcag tgggcgttct
     3241 ggacgtcttc tcccgcgcgt gtgagcaggc gatctccgct ttcggcgccc ttttcggtgg
     3301 ctggcgaccg gatggttggc cgcgcggtgt ccaggaggag aaccgtgagc gcccatgggg
     3361 caagaggcgt ccgggaccag ctcgcccgac gctgccgacg cccagcctca cccgggtgcg
     3421 accagccgtc cacatccgtt agggctagag gccggccgtc cctcgaatcg cgtcaggcag
     3481 gtacgggctg tcgaagtaca tcagcccggc gcgctcgacg atctccctgg cctgcggcga
     3541 cccactttgc agccatacgg cgcgcgcatt gagcgcgcgc gcctgttcga cgatgcgctc
     3601 gagctccgcc agaggccggt gggcgtagac gatgtcgatc atgtgcggcg cggcgtcaag
     3661 tgggcggacg cgaacctcgc cgtccgccag ctcgtacgcg ttgtactcgt cgtcgcgcgg
     3721 cccgtcggcg gacgcgaccc gatagccgtg ccgcgccagg gtgtccggca catcgcgact
     3781 gggccagtcg atgagcagga atgatgtggc cgttgcaagc agctcgtggt agtcggtcat
     3841 ctgatcggtg tccatggtca cacatacaat cggccggatg acggtttatc tggatcacgc
     3901 tgcgacaacg ccgctgcggc gcgaggtcct cgacgcgatg atgccgctgc tcacgagcga
     3961 tttcggcaac ccgtcaagcg ttcacgggca cggccgtaag gcacgggcgg cactcgatga
     4021 gacgcacgag cgcctggccc acgggctcaa cgcgcaaccg cgggagatca tcttcacggg
     4081 cggcggcagc gaggccatca acctagcgct caagggtgtc gcgtgggcgg gcaagggccg
     4141 cggcaaccgg ctgatcacga gcgcggtcga gcaccacgcc gtgctgcact cgctacgcca
     4201 tctcgagaag ttcggcttcg agatcatcgt gctgccggtc gaccgctacg ggcggctgga
     4261 ccccgaacag ctcgataacg ccatcaacga ccgcacgatc ctggtgagcc tgatgctcgc
     4321 caacaacgag gtcggcacga tccagccgct cgccgacctg gtgcgccgcg tgcgcgccca
     4381 ccggggagtg gcaatccacg tcgacggcgt gcaggcc
//
LOCUS       DATBRX010000130        22153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_265679, whole genome shotgun sequence.
ACCESSION   DATBRX010000130 DATBRX010000000
VERSION     DATBRX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 22153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22153
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265679"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1355)
                     /locus_tag="VIF08_04695"
     CDS             complement(36..1355)
                     /locus_tag="VIF08_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8135318.1"
                     /translation="MNEPPVYLEPVAPSPRPASALAGLLLLVVVFVVGVSVGQSGFLA
                     AATTTPQPTATPGLENMDLFWQALQDIRTRYVGRGELSDQTLLYGAIRGMVDSLGDTG
                     HTTFLTPEQVKLLQDSLNQSVVGIGVTLAQRDGAIVVTSVLPDSPARDAGIQTGDVIR
                     AVDGASVAELGLEELISRVRGEAGTSVEISLVRPSTGEALELTIVREELHVPAAAWAF
                     VPTTHVALLRLSSFGTGSGAELQAARDAAVAGGATALILDLRSNPGGYVHEAVNVASQ
                     FLTGKTVYISEDASGARTPVTTDDAVVATDLPLAVLVDQYTASSAEIVAGAIQSAGRA
                     PVVGETTFGTGTVLEVVDLGDGSQIRLATSRWLTPDGELIFGKGITPDEVVTLDGVYD
                     TPVDPEALAVPDQAAALNAALDDPQLLKALELLGVSLPTREGPAPSA"
     gene            complement(1699..2922)
                     /locus_tag="VIF08_04700"
     CDS             complement(1699..2922)
                     /locus_tag="VIF08_04700"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135319.1"
                     /translation="MSIDIRTVPADQLRPWAEAVNVAFGEPIDEAQWRLDQTLFEPER
                     VLGAYDGDALVGGGAAFSFRMTVPGGARVRTAGVTMVGVMPTHRRRGILRSVMARQLA
                     DVRRNDEPLAALVASEGGIYQRFGYGLAILQGAIEIERDRAQFRMDSPAVGSVRLVDR
                     EEARRLMPLAYDPVCAATPGFIERDPAWWDERLADIESQRRGAGPQYRAIYERDGEVR
                     GYVRYRIKSEWGTVASNSTLIVNELIAMDPVAEHELWRYCFGVDLIARIQYRWGPPSH
                     PLLLMLAEPRRLQLRVSDGIWLRIVDVPAALAARGYAADGTLVLDVRDDFVPDAAGRW
                     RMSVEGGAAKVEPTADSPDLALDTTDLAAAYLGTFSFSELARANRTEELTTGARVRAD
                     LMFATAVLPWCSTPF"
     gene            complement(2935..3219)
                     /locus_tag="VIF08_04705"
     CDS             complement(2935..3219)
                     /locus_tag="VIF08_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135320.1"
                     /translation="MDEGRLDRLEQRLEDMDRRSSAMERMLEMLLDRSRQAADVIMPT
                     KTRGHLRAAGRENLLAIRSLLDFWADKLADPSDDSDAPADQSKRQNIPID"
     gene            complement(3212..4411)
                     /locus_tag="VIF08_04710"
     CDS             complement(3212..4411)
                     /locus_tag="VIF08_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEY8135321.1"
                     /translation="MTKILLYTGKGGVGKTSIAAATAVLCARRGLRTLVVSTDIAHSL
                     ADALDTPVGPTPRQIEPNLWAHEPDVFFNISRYWRTISSYFSSLFSWGGLDEVMAEEM
                     TVLPGMDEIGNLLWIGDHVDKAEYDLIVVDAAPTGETVRLLSLPEASRWWIEKIAPIG
                     RRVQKLGGPVLRRIVGVPLPTDEVYQSAELLLERLRYVHDLLADPDRSSVRLVLNLEK
                     VSIQEAQRSFTYFHMYGFPTDLIVANRVLPAASGAYFDAWREAQARYLPMVEQQFAPV
                     PVRTVPFFDKEMVGVERLAELGQALFGDSDPHQFLYRGRPYSVSRDEAGYTLALELPF
                     ASKDEISLSRNGDELVLQVGSWRRNLVLPRLLLDAPTQGAKMEDRVLKVRFAEPPRAK
                     IGGSRNG"
     gene            complement(4497..5123)
                     /locus_tag="VIF08_04715"
     CDS             complement(4497..5123)
                     /locus_tag="VIF08_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8135322.1"
                     /translation="MRLLAVSDETDRALDIEANRVALGRIDGIVGAGDLEPDYLSFLA
                     DAFHAPLVYVRGNHDRGGNWDARGPVAPQPLGPSTEALCGLRVAGLSWPGEMRGQAIR
                     DEGAAWRQALGLWLRLRGARPEIVLSHVPPRGLGDVPEDNYHRGFAAYRWLCRRLAPR
                     LWIHGHTAPSAVAAWRHDWGGTTFVNATGAVLIEFAAVPARASMELGS"
     gene            complement(5168..5839)
                     /locus_tag="VIF08_04720"
     CDS             complement(5168..5839)
                     /locus_tag="VIF08_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135323.1"
                     /translation="MTTDKDAPSQPEEGRSVLADVAANIVDAASPVFAAARRVIDERP
                     GARVRRVRRLGHQRLPNLWEEHPEAQQAAIRELGLLSVPVAEIKGTAVAGPAQRGGDF
                     LPLRDRRGDDWQARWRRILDANERLEPLPPVELIKFADAYWVVDGHNRVAAALYIGQN
                     EIDAVVRELRLPGMPIETKPPIASVLEGSLDLRAAGEGRLTRTADRPLDLESARPHEH
                     TDEAE"
     gene            5926..6075
                     /locus_tag="VIF08_04725"
     CDS             5926..6075
                     /locus_tag="VIF08_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135324.1"
                     /translation="MIEQTCPWCEAKIEVELRVLTGDESETCPECLTTWAFEDEPVYE
                     LALAA"
     gene            complement(6072..7169)
                     /locus_tag="VIF08_04730"
     CDS             complement(6072..7169)
                     /locus_tag="VIF08_04730"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8135325.1"
                     /translation="MIGRDLITRAPKVLLHDHLDGGLRPTTVIELARDCGYDGLPTTD
                     PGELARVFTAGADRKSLVLYLEGFDHTVAVMQNRDAIIRVAQECAEDLAADGVVYAEV
                     RYAPELSTRDGLSLDEVVDAVLDGFRRGHASSGIEMGFIVTAMRQFARSVEIAELAVR
                     HRDEGVVGFDVAGPEKGFPPTRHLDAFNLIHQANFNLTIHAGEAFGLPSIWEALQWCG
                     AHRLGHGVRIVDDITVDADGQVRMGRLASYVRDMRVPLEMCPTSNVHTGAASTIEEHP
                     IDLLRRLRFRVTVNTDNRLMSGVTMSSEFETLSRTFGFGLDEMQWLTLNAMKSAFAPF
                     DQRLRLINGVIKPGYARLLAETEFAETAARY"
     gene            complement(7203..7727)
                     /locus_tag="VIF08_04735"
     CDS             complement(7203..7727)
                     /locus_tag="VIF08_04735"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8135326.1"
                     /translation="MPRKIVGLQIYLAHGASGNAASMQPHVAGLRKRGFEGHAVDLPV
                     GLAEKAVGRYRDQVADLASAIIGGQSYGGRVATLLAADEPPRGLVLLCFPLHRPGQPD
                     GTGRVGHFPRIECPALFLSGEADPFARIDLLRAAVKALPDGRLITYPRLGHSLRPVLD
                     DALDRVAEFARTLS"
     gene            7835..9052
                     /locus_tag="VIF08_04740"
     CDS             7835..9052
                     /locus_tag="VIF08_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135327.1"
                     /translation="MDVEGDVIVIDHLRVEDRALAAFVGSRAESDRAEMADRALRIGL
                     HALQDAGTALDVEFVRREFDALLERNASVNERAAKELDQVLRLNFGDREGRLPRTLEQ
                     FLGDHGQLTRLVAELFDESKRDSAIGRLRTLLGTYFDGDASRLAQLLDPTRMGAPLYQ
                     FRTEMNAAFEKLNDRLTAIEAASAARAAERSKSSAKGGDFEDLLEEMLGEAVRGTGDT
                     VERTGTAAGDVIRSKKGDFLLTIDPQWCGGAELRVVIEAKNRGKSWREMREELNEAKR
                     NRGAAAALAVYTPEHAPAGVAPFDVRFGHVFAVIDPESPDMATLTAAVRLARLHALAC
                     VAVQTTEVDTARIAAALAALKTELDAVRALKMSLTEIGKTANNVSVGLDRLRDQVLGR
                     VSEAEAQLKPKSV"
     gene            complement(9146..9637)
                     /locus_tag="VIF08_04745"
     CDS             complement(9146..9637)
                     /locus_tag="VIF08_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8135328.1"
                     /translation="MLPLNHPASVDSETVPQTEVHGPSPVKIRTVLLATDLTPASEAA
                     TVQAISMAASLGARLLVVNVIDQGGPVSPLATVRVDQQRALREGPLLQIVNRARARGV
                     EAAFLLWTGEPGQGIVAAAEAEGADMVIVGTRGLDRAGRFLLGSVSDYVVYHSSCPVM
                     VAR"
     gene            9787..11301
                     /locus_tag="VIF08_04750"
     CDS             9787..11301
                     /locus_tag="VIF08_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8135329.1"
                     /translation="MAAADLSLLPVHEFIVLLEDWRDEPTDAQRERLRESLGRIRAAY
                     GGRGVRLKLHSAHMPELDIANGTFTGSNDGDAPTGAASDLAVEPMARGEALLWAEGSD
                     ESVEAISAALQLAIDSIWSKHEARLRRRQLEALDLAVRGIAGVLPPERVLQLIVDRVR
                     ELVDAQYAALGIVGSFGRIEQFVTSGLTDEVRAHIGNLPRGLGLLGLIIREDRSFLID
                     DIATDDRRYGFPEHHPEMHSFLGVPVRSKGQSIGNLYLTNKRGGAPFNEADLRLVEMF
                     ALHAGIAMENARLHEEIGRLAIVQERERISQDLHDSIIQSLYGISLSLEDLPEIVSED
                     PAEGTARTDHAIDGIHATIRDIRNFIMGLQPELLMDADLSTGVETLAAEFRANTLIDL
                     ELHVDPDLPELPRDHAAHILAITRETLSNIARHSSATRAAIQLFAKDGTATLIVGDNG
                     RGFDVEAARSSRQRGLGNLRARAEQVGGRLILASEEGSGTRVTAELPLNREDQV"
     gene            11298..11981
                     /locus_tag="VIF08_04755"
     CDS             11298..11981
                     /locus_tag="VIF08_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8135330.1"
                     /translation="MTAAHEGGERTEIQHRLLVVDDHEVVRQGLVAMLERRAGFQVVA
                     EAGTAADAIEMARRHQPDLVVMDVRLPDGSGIEACREIRAEMPNTRVVILTSYPDEEA
                     VFSAIVAGASGYLLKQVRGRDLVAALEAVGRGESLLDPAVTEKVLERVRRIATGGATD
                     ELAQLTAQEQKILLLVAEGKTNKEIAAEVFLSDKTVKNYVSSILSKLNLQRRAQAAAY
                     LAKRHGGSS"
     gene            complement(11978..12418)
                     /locus_tag="VIF08_04760"
     CDS             complement(11978..12418)
                     /locus_tag="VIF08_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY8135331.1"
                     /translation="MGRTMPMRLQLTRRGDYAIRAMLLLAQESDVLTGADIARRTHIP
                     GRLVGQVMGQLVRAGLVHARLGRHGGYWLPPQGGATSILAIVEAVEGDARRQRCVLSG
                     TQCDVANRCQVHDIFASAQGALINRLAQASLDSAAAPLQRRGRA"
     gene            12538..14100
                     /locus_tag="VIF08_04765"
     CDS             12538..14100
                     /locus_tag="VIF08_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8135332.1"
                     /translation="MKATFRALILVVVVISLLAACTTGPRPAWTYTPATPSPGSSAQP
                     SGAPPTAVPNSDDDVLPALTAKADLGAVPAGEVNVAYAPEVPPAAARTTQAIVEVAFD
                     VVEGVKAIGPNGLEYTTWGYRLHGDSAVTTGTPGPIIRARVGDLLRFSLTNNGTMPHN
                     VDFHAVTGQGGGAKDTTVNPGETAIIEARLLYPGMFMYHCAAGDVPQHIAQGMYGGIL
                     VDPETPLAPVEHELYMVQSEFYTNDPAAGPLTTDRDAITDEHPQFVVFNGAVGALAGD
                     NAPKMQVGERMRIYFVNAGLNLDSNFHPIGSHWDRVWTEGALLNEPLRGSQTTLVPAG
                     GSTIVELIGLVPMTVVLVDHALARAFDKGAIGQIVITGDPNTEIFESIGKSGTPLPTT
                     VPTTSPVPSTTPQGSPLPTGTPGEGFHVDIVAGAFTFQDANAADEYADTESPADYSIN
                     VLMVPLGSTVTWTNHDPGQVHTVTAVDGSFDSGFLNEGQTFSLRFDTPGEFEYFCGPH
                     PWMRARIMVEMH"
     gene            14211..15482
                     /locus_tag="VIF08_04770"
     CDS             14211..15482
                     /locus_tag="VIF08_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8135333.1"
                     /translation="MRLVTAAEAVAGIRSGEQLYIHSAAAAPSVLLDALVARASELSD
                     VRILHFHTEGPGPHLTPEMAPHFRHRALFIGPNARKAVNEGRAEYIPAFLSDVGTLIR
                     RGVLPIDTVFVNVSPPDKHGFCSLGTSVIAMPTAIRYAKQVIAQVNSAMPRTLGDSFV
                     HVDDIDLAVEVDVPPYATAPAVVGDVERRIGDFVADLVPDGATIQLGIGAIPGAVAAA
                     LQGKRDLGIHTEMMTDVVREMVEAGVVTGRAKEINYGRVVATFMLGSQRLYDWADNNP
                     MVEMRPADYTNDTAVIRRFKKMVAINSAIEVDLTGQVCADSIGPRMYSGVGGQMDFIR
                     GAALADDGRAIIALPATTSDGTISRIVPYLREGAGVVTTRAHVETIITEYGVAEMHGR
                     SIPERARQLIAIAHPDLRPWLEEEARRAHYI"
     gene            15681..16361
                     /locus_tag="VIF08_04775"
     CDS             15681..16361
                     /locus_tag="VIF08_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135334.1"
                     /translation="MSEVGWLLVHGSPFEHGPAYLLVAIRPRPTRAHFDPERIECWCP
                     GSAAAEQATLEWPIAKPPPTYSWGTIKVIDRVAAVNKFASFGGSLSGVRDGQLSAALF
                     RSEAPILAVSGRGDAGDPLAASVVGFFARLAAACGADSELSRDVSAMDPLVLYAAYLV
                     RALELYGGDLAALAAPPRLQALLRSELCLLERDSGASAAAGEALARRLDGAGQERKGV
                     RSWSARFG"
     gene            16340..16801
                     /locus_tag="VIF08_04780"
     CDS             16340..16801
                     /locus_tag="VIF08_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8135335.1"
                     /translation="MVGTIRMTAADFAFARYEHAQLRAQLDDIRAAADTVGRLSPLEA
                     ARAVSRVRAWLATTLGPHAAWENLVVYPAIERGTATTAATNVMRSEHRQIEHMGFVLE
                     ADADLLRAGPVTHDLVCDIRAHLLGLQSILCTHMEREDEFLRPEIDSPDQR"
     gene            16807..17718
                     /locus_tag="VIF08_04785"
     CDS             16807..17718
                     /locus_tag="VIF08_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8135336.1"
                     /translation="MDNTAMRVLVATDGSEAADRAVGLASSIEWPAGTKIRVVTVIEP
                     VPPVPAAEWVGAAAPVEAMYPHEADRVAKAILERACDALAHTSAEVSGQMLYGRPATS
                     ILESARELGADLAIVGSRGHGTIASMVLGSVGAEVADLAECPVLIARGRHLTRAVLGV
                     DDSEFSRAAEKVVAEWPIFAKVAIEVTHVAQVALPWMVALGPGVPVQPEIGPDEYETV
                     GAATRRLKEAGRHAFGRMVVGDPAAGLLLTAEEAQADLIVVGTHGRTGLSRAVFGSVA
                     RNVMLHAPCSVLVVRRIREGQTQLKAA"
     gene            17836..19806
                     /locus_tag="VIF08_04790"
     CDS             17836..19806
                     /locus_tag="VIF08_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY8135337.1"
                     /translation="MNAAHPAYPGIPAIIDGSEAVAHVETRLAEACCAYPITPSTNMA
                     TLFQAAVADGKTNLWGTRLSFLEPESEHSSASAAEGFALAGGRVTNFTAGQGLLLMKE
                     VLFTISGKRLPAVFHVGARALTSQSLNIHAGHDDVMSVADAGWGILFARNVQEAADLT
                     AIARRVAEETETPFLVVQDGFLTTHTLENALLPEDELLREFVGDPRLRIRNLFEPSEA
                     LMTGVVQNQDSYMKGRVAQRAWYERIPAVMDAVMNEWAELTGRRYGAIDAYLTREAEH
                     VVVSMGTIADSARDVVDHLRATGVRAGGVTVTSFRPFPATQIAAALADARSVAIVERT
                     DEPAAAANPLTRETKSALADLAAAGRSIPRVVSASAGLGSRDVASADLLAVFDWLRDP
                     SAMDERPYVVLGIKHRLGLAAPDIELRPTGAYSLRGHSIGGYGSVTVNKLLASIVGQT
                     FGLYVQAYPRYGSEKRGLPTTYYLTTAESPIRQHSELRRVEFVPLHDVAAFGQGAPLS
                     GLVDGGTLFVNSTLDDPAGIWAALPANARAEIIGRGINVVALDTLALAKRFAPTPNLA
                     TRMQGAALVGVFLRVAPFIAGRGLSLDDLMAAVKGPLAKHVGKRGGAVVDANLALIRA
                     AYEGLIDVTAPLRAAPPQAAGEGAREMAGVTR"
     gene            19803..20246
                     /locus_tag="VIF08_04795"
     CDS             19803..20246
                     /locus_tag="VIF08_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8135338.1"
                     /translation="MSAELEVVHDEADVPLVGQLMTPDPIVVEDGASLDEAVRLLEEN
                     EISGLPVVDRDGLLVGVLSQTDIIHARAVAHLWSRWPGLRVRHLMHAPALTADREMTM
                     EEAAQIMERSHVHRLIVVDEDQLKPVGVISTTDLVRALAHRPTDG"
     gene            20239..21915
                     /locus_tag="VIF08_04800"
     CDS             20239..21915
                     /locus_tag="VIF08_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8135339.1"
                     /translation="MVELDTARPAVDLVDYDRALPVFDVGSYVEEFNESVMPAYKSGT
                     ADREYAADNGLARSIIPPGTAATRDFSTLASRIPQLLADACVGCMACVSACPDTAILG
                     TAQPRAALKERIAEFSAANANGVAADVIALDLTAQFVATQKYATVPERRGLEPADFGI
                     FIDPTNCKGCAECVEVCAHLDHNALVMIDKEAPTDRSEGTLEPYRRRMSFFRSLPTTP
                     VEYRNEKALADLMLGEHALGYVGGAGSCSGCGEATALRMMVAATRQVHGPESMGIVAA
                     TGCNTVYGSTYPFNPYMVPWTNSLFENAAADAMGIRARWDQAGHTDRRLWAVGGDGAM
                     YDIGFQALSRLVASGADVKVLVLDTQAYSNTGGQSSTATFGGQVTKLSAFGKEIHGYP
                     ARRKELGRIMLAHDQVFVAQTTTAHLNHFYRAVIDANEFPGPAVIVVYTPCQPEHGIG
                     DEAAAQQARFAVDSRAFPLFSYDPRRGPSLAERLSLQGNPALREDWLHTPDGTVTDFL
                     AFARTEGRFAPHFAADGSPSIEIQATNADRLENWRLLQEMAGLRKEGAAQ"
     gene            21938..>22153
                     /locus_tag="VIF08_04805"
     CDS             21938..>22153
                     /locus_tag="VIF08_04805"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEY8135340.1"
                     /translation="MHVRRGFLNWGIFLVCLGAVPLAVQLNVIDRDVATSLLRLWPLI
                     LIGIGLGIMLRFSRAAALGGIVVAATFG"
BASE COUNT         3627 a         7548 c         7525 g         3453 t
ORIGIN      
        1 ggcggcggag ccagcgacgc gagcggcccg gttcattagg cgctgggcgc cgggccctcg
       61 cgcgtcggca ggctgacgcc cagaagctcg agcgccttca gcagctgcgg atcatccaga
      121 gccgcgttca aagccgcggc ctgatcgggg acggcaagcg cttccggatc gaccggcgtg
      181 tcgtaaacgc catcgagcgt taccacctcg tcgggcgtga tgcccttacc gaagatgagc
      241 tcgccgtccg gcgtcagcca gcgtgacgtg gccagcctga tctgcgagcc atcgcccagg
      301 tcaacgactt caagcacggt gccggtgccg aaggtggtct cgcccacgac gggcgcgcga
      361 cctgctgatt ggatggcccc cgcgacgatc tccgccgagc tggccgtgta ttggtcgacc
      421 agcacggcca gcggcagatc ggtcgcgacg accgcatcgt cggtcgtgac cggcgtccgg
      481 gcgcccgacg cgtcctcgct gatgtagacg gtcttgccag tgaggaactg gctcgcgacg
      541 ttgaccgcct cgtggacgta gccacccggg ttggagcgca ggtccaggat cagcgccgtg
      601 gccccgccgg caacggcagc gtcgcgcgcc gcctggagct cggcgccgga gcccgtgccg
      661 aatgatgaaa gccgcagcaa cgcgacgtgc gtcgtcggga cgaacgccca tgccgccgcg
      721 ggaacgtgca gctcctcacg cacgatcgtg agctcgagcg cctctccggt cgatggccgc
      781 acaagcgaga tctcgacgct cgtccccgcc tcgccccgga cccggctgat cagctcctcg
      841 aggcccagct cggcaaccga tgcgccatcg acggcacgga tcacgtcgcc cgtctggatg
      901 ccggcgtcgc gagccggact gtccggcagg accgaggtga ccacgatcgc gccatcgcgc
      961 tgggcaagcg tgacgccgat gccgacgacc gactggttga gcgagtcctg cagcagcttg
     1021 acctgctctg gcgtcaggaa ggtcgtgtgc ccggtgtcgc ccagcgaatc gaccatgccc
     1081 cggatcgcgc catagaggag tgtctggtcg ctgagctcgc cccgccccac gtacctggtg
     1141 cggatgtcct gcagcgcctg ccagaacaga tccatgtttt cgaggcccgg ggtcgcggtc
     1201 ggctgcggcg ttgtcgtcgc tgcggccagg aagccgctct ggccgactga tactcccacc
     1261 acgaagacga ccaccaacag cagtagcccc gccagcgcgg acgcgggtcg ggggctgggc
     1321 gcgaccggct cgaggtagac cggcggctcg ttcaagggat gcgcctcatg gtcgggagat
     1381 catgggccag agcggcggac ggcgctcgca aggcgaggtc gaagcaccgc gcgagcgggg
     1441 catcgactga ggagcgcagc agcgccccgg cggggcggtg cggagagagt ccctgtggac
     1501 gaagttctga acacggcgtg gacaaggtcg accggccatt ccaacttcgt ggacaaaggc
     1561 cgttgccggg cctggcgggc ggcccgtagt ctctcgtcgt cccgaagggg ccacggcacc
     1621 ggggccgccc tcgctggctt gccagcggag acggcaactg gaccgggcgg ctccttcggg
     1681 cgttttgttg tcccctcgtt agaagggggt gctgcaccag ggcaggacgg ccgtcgcgaa
     1741 cattaggtcc gcgcggacgc gggcgccggt ggtcagctcc tcggtccggt tcgcgcgggc
     1801 cagctcgctg aagctgaagg tgcctaggta ggccgcggcg agatcggtcg tatcgagcgc
     1861 gaggtcgggt gaatcggcgg ttggttcgac cttcgctgcg ccgccctcca cggacatgcg
     1921 ccagcgcccg gccgcatccg gcacgaagtc atcgcgtacg tcgagcacca gcgttccgtc
     1981 cgccgcgtag ccgcgcgccg cgagcgcagc ggggacatcc acgatgcgca gccagatccc
     2041 atcgctgacg cgcagctgca ggcgccgcgg ctcggcgagc atcagcagca gcggatggct
     2101 gggcggtccc cagcggtact ggatgcgcgc gatgagatcg acgccaaagc agtagcgcca
     2161 cagctcgtgt tccgcgaccg ggtccatggc gatgagctcg ttgacgatga gcgtgctgtt
     2221 gctcgccacc gtgccccact cggacttgat gcgatagcgg acgtaaccgc gcacctcgcc
     2281 gtcgcgctcg tagatcgccc ggtactgggg acccgcgccg cggcgctggg actcgatatc
     2341 ggcgagccgc tcgtcccacc acgccgggtc gcgctcgatg aagccgggcg tcgcggcgca
     2401 taccggatcg taggcaagcg gcatcaggcg ccgcgcctct tccctgtcga cgagccgcac
     2461 tgagccgacc gctggggaat ccatacggaa ctgcgcgcga tcacgctcga tctcgatcgc
     2521 accctgcagg atggccaggc catagccgaa gcgctggtag atgccgccct ccgacgccac
     2581 gagggcggcg agtggctcgt cgttgcggcg tacgtccgcc agctggcggg ccatgacgct
     2641 gcgcaggatg ccgcgccggc ggtgcgtggg catgactccg accatcgtca cgcccgcggt
     2701 ccggacgcgc gccccgccgg gcacggtcat gcggaaggag aaagccgcgc cgccgccgac
     2761 gagggcgtca ccgtcatagg cgccgaggac ccgctcgggc tcgaagagcg tctggtcgag
     2821 ccgccactgg gcctcgtcga tcggctcgcc gaaagcgaca ttgacggcct ccgcccacgg
     2881 ccgcagctga tcggccggga cagttcgtat gtcgatgctc accgggttga ggagttagtc
     2941 gatcgggatg ttctgccgct tcgactggtc ggctggggca tccgagtcgt cgctcgggtc
     3001 tgccagcttg tcggcccaga agtcgaggag gctccggatc gcaagcaggt tctcgcggcc
     3061 ggccgcacgc aggtgaccgc gcgtcttggt gggcatgatc acgtcggccg cctggcgcga
     3121 ccgatcgagc aacatctcga gcatccgctc catggcgctg ctgcgccggt ccatgtcctc
     3181 caaccgctgc tcgagtctgt cgagccgacc ctcatccatt tcgcgaccct ccgatcttgg
     3241 ctcgcggggg ttccgcgaaa cgcaccttca aaactcggtc ttccattttc gcgccctggg
     3301 tcggcgcgtc gagcaacagg cgcggcagga cgagattacg gcgccacgac ccaacctgca
     3361 gcacaagctc gtcgccgttg cgcgagagcg agatctcgtc cttggacgcg aagggcagct
     3421 ctagcgccag ggtatagccg gcctcgtcgc gcgacaccga gtagggccgg ccgcgataga
     3481 ggaactggtg tggatcggag tccccgaaca gcgcctggcc aagctcggcg aggcgctcga
     3541 cgccgaccat ctccttgtca aagaagggca cggtgcgcac cggcactggc gcaaattgtt
     3601 gctcgaccat gggcaggtag cgcgcctggg cctcgcgcca cgcgtcaaag tacgcaccgc
     3661 ttgctgccgg caggacgcga ttggcgacga tcaggtccgt cgggaagccg tacatgtgga
     3721 agtaggtgaa cgagcgctgc gcctcctgga tggagacctt ctccaggttg agcacgaggc
     3781 gcaccgacga gcggtccggg tcggccagga ggtcatgcac gtagcgcagc cgctcgagca
     3841 gcagctcggc cgactgatag acctcatcgg tcggcagcgg gacaccgact atccgccgga
     3901 gcactggacc accgagcttc tgcacgcggc ggccgatggg cgcgatcttc tcgatccacc
     3961 agcggctcgc ctccggcagg gaaaggagcc gcaccgtttc gcctgtcggc gcggcgtcaa
     4021 ccacgatcag gtcgtactcg gccttgtcga cgtggtcgcc gatccacagc aggttgccga
     4081 tttcgtccat acccggcagc acggtcatct cctcggccat cacctcatcg aggccacccc
     4141 acgagaagag gctcgagaag tacgaggaga tcgtgcgcca gtaccggctg atgttgaaga
     4201 agacgtccgg ctcgtgcgcc cacaggttgg gctcgatctg gcgcggcgtc ggaccgacgg
     4261 gcgtgtcgag cgcgtcggcc aggctgtgcg cgatgtcggt cgagacaacg agggttctca
     4321 ggccacggcg cgcgcacaac acggcggtcg ccgcggcgat gctcgtcttg cccactcccc
     4381 ccttgccggt gtagagcagg atctttgtca actgtttccc tccgcagctg ccgctgctgc
     4441 gcgcgaagca ggcgacccgc agcatcctgc tgcgtttcgc cagtcgcttg ctcctctcaa
     4501 cttcccagtt ccatgctagc cctcgcgggc actgcggcga actcgatcag cacggcgcct
     4561 gttgcattga cgaacgtcgt gccgccccag tcgtggcgcc aggcggccac ggccgacggc
     4621 gccgtatggc cgtgaatcca gagccgcggc gccagcctgc ggcaaagcca gcggtacgcc
     4681 gcgaagccgc ggtgatagtt gtcctctggc acgtcaccca ggccgcgcgg cgggacgtgg
     4741 ctcaggacaa tttcgggacg tgcgccacgc agacgaagcc acaggccgag tgcctggcgc
     4801 caggccgcac cttcgtcccg gatggcctgg ccgcgcatct cgccgggcca ggaaagaccc
     4861 gcaacgcgaa ggccgcacag tgcctcggtc gaagggccca ggggctgcgg cgcgacagga
     4921 ccgcgtgcat cccagttccc gccccgatca tggttgccgc gcacgtagac cagcggcgca
     4981 tggaacgcgt cggccaggaa ggacaggtag tcgggctcga gatcacccgc gccgacaatg
     5041 ccatcgatcc ggcccagggc aacccggttg gcttcgatgt cgagcgcgcg atcggtctca
     5101 tccgacaccg ccagcaggcg cacgtcatcg gccaatcgcg cgggccgggc caggcgcagg
     5161 cggcgaatca ttcggcctcg tccgtgtgtt cgtgagggcg cgctgactcg agatcgagcg
     5221 gtcggtcggc agtgcgcgtc agtcggccct cgcctgccgc tcgcaggtcc aggctgcctt
     5281 cgagcaccga cgcgatgggc ggcttcgtct ctatcggcat gcccggcagt cgcaactcgc
     5341 gaacgacggc gtcaatctcg ttctggccga tgtacagcgc agctgcgacg cggttgtggc
     5401 catccacgac ccagtaggca tcggcgaact tgatgagctc aaccggcggt agcggctcga
     5461 ggcgctcatt ggcatcgaga atgcgtcgcc agcgggcctg ccagtcgtcg ccccgccggt
     5521 cgcgcaacgg caggaagtcg ccgccacgct gcgccggacc ggcgacagcg gtgcccttga
     5581 tctcggcgac cggaacactg agcaggccga gctcgcgtat cgccgcctgc tgcgcctcgg
     5641 ggtgctcctc ccataggttg ggcagtcgct ggtggcccag ccggcgcacg cggcgcacgc
     5701 gcgccccggg ccgctcgtcg atcactcgcc gcgcggccgc gaacaccggc gaagcggcgt
     5761 cgacgatgtt tgccgcgacg tcggccagga ccgagcgccc ctcctcgggt tggctcggag
     5821 cgtccttatc ggtggtcaca gcgcgaggct aactcgtttg cgccgcgtga cacacaagct
     5881 gtcattcacg gctgaaaacc ttggcgcgca aaggaggtga gcagagtgat cgaacagacc
     5941 tgtccgtggt gcgaggcaaa gatcgaagtc gagttgcgcg tcttgaccgg tgacgaatcg
     6001 gagacatgtc cggagtgcct gacgacgtgg gcgttcgaag acgagcccgt gtacgagttg
     6061 gcgctggccg cctaatagcg cgcggcggtc tccgcgaatt cggtctcggc gagcagccgc
     6121 gcgtagccgg gcttgatcac gccgttgatg agccgcagtc gctggtcgaa tggcgcgaaa
     6181 gcgctcttca tggcattcag cgtcagccac tgcatctcgt ccaggccaaa gccgaacgtg
     6241 cgcgacaggg tttcgaactc ggagctcatg gtcacgccac tcatcagtcg gttgtcggtg
     6301 ttgaccgtga cccggaaccg cagccgccgc agcaggtcga tcgggtgctc ctcgatcgtt
     6361 gacgcggcgc ctgtgtggac gttcgaggtt ggacacatct cgagcggcac gcgcatgtcg
     6421 cgcacgtagc tcgcgagccg ccccatgcgc acctgaccat ctgcgtcgac cgtgatgtca
     6481 tcaacgatgc gcacgccatg gcccaagcga tgcgcgccgc accactgcag cgcctcccag
     6541 atggacggca gaccgaatgc ctcgccggcg tgaatggtca ggttgaagtt ggcctgatga
     6601 atcaggttga acgcatcgag atggcgagtg ggcgggaagc ccttctccgg gccggccacg
     6661 tcgaaaccga caacgccctc atcccggtgg cggacggcca gctcggcgat ctcgaccgag
     6721 cgcgcgaact ggcgcatcgc ggtgacgatg aagcccatct cgatgccact cgacgcatga
     6781 ccgcgccgga agccgtcgag cacggcgtcc acgacctcgt cgagcgacag cccgtcgcgt
     6841 gtcgatagct cgggcgcgta gcggacttcg gcgtagacga cgccgtcggc cgcaagatcc
     6901 tcggcgcatt cctgggcgac gcggatgatc gcgtcgcggt tctgcatcac cgcgacggtg
     6961 tggtcgaagc cttccaggta caggaccagg ctcttgcggt cggcacccgc ggtgaagacg
     7021 cgcgccagct cacccggatc ggtggtcggc agaccgtcgt agccgcagtc gcgcgccagc
     7081 tcgatgaccg tcgttgggcg cagcccgccg tctaggtggt cgtgcagcag caccttgggc
     7141 gccctggtga tcaagtcgcg gccgatcatg agttcggcgc aaggttatgc gccggcgccg
     7201 ccttacgaaa gggtgcgcgc gaattcggcg acgcgatcga gcgcgtcatc caggaccggc
     7261 cgcagggagt ggcccagccg gggataggtg atgaggcgcc cgtcgggcaa cgccttcacg
     7321 gccgcacgca gcaggtcgat ccgcgcaaag ggatcggcct cgccggaaag gaacaacgcc
     7381 gggcactcga tgcgcgggaa gtggccgacg cggccggtcc cgtctggctg ccccggtcgg
     7441 tgcagcggga agcacagcag cacgaggccg cgcggtggct catcggccgc gagcagagtg
     7501 gcaacgcgac cgccatagga ctggccaccg atgatcgcgc tcgccaggtc ggccacctgg
     7561 tctcggtaac ggccgacggc cttctccgcc aggccgaccg gcaggtcgac cgcgtgccct
     7621 tcgaacccgc gcttccgcag gccggccacg tgcggctgca tgctggcggc gttgcctgac
     7681 gcgccatgcg cgaggtagat ctgcagcccg acgatttttc gcggcatctg cagatcgtga
     7741 cacgctaggt gtcacagcgt gcgtggaacg tcggcgcatg gaaaccgccc agatagtcgc
     7801 actccgggtt gccccgcaac gctcgcgccc cactgtggac gtcgagggcg atgtaatcgt
     7861 catcgaccac ctgcgggtcg aggaccgggc gctggctgcc tttgtcgggt cacgcgcgga
     7921 gtccgaccga gcagaaatgg ctgaccgggc gctgcggatc ggcctgcacg ctctccagga
     7981 cgcgggcacc gcactggacg tggagtttgt gcggcgcgag ttcgacgcgc tgctcgagcg
     8041 gaacgcgtcg gtcaacgagc gcgccgccaa ggagctcgac caggtgctgc gcctcaactt
     8101 cggcgaccgc gagggccgct tgccgcgcac cctggagcag ttcctgggcg accacggcca
     8161 gctgacccgg ctcgtggccg agctcttcga cgagagcaag cgcgacagcg ccattggccg
     8221 gctgcgcacg ctgctcggca cgtacttcga cggcgacgct tcgcgcctgg cgcagctgct
     8281 ggacccgacg cgcatgggcg cgccgctgta ccagttccga acggagatga acgccgcctt
     8341 cgagaagctg aacgaccggc ttacggccat tgaggcagcg tcggccgcgc gggccgccga
     8401 gcgctcaaaa tcgtcggcca agggtggcga cttcgaagac ctgctcgagg aaatgctggg
     8461 cgaggccgtc cgcggcaccg gtgacacggt cgagcggacc ggcaccgccg cgggcgacgt
     8521 catccgctcc aagaagggcg acttcctgct gacgattgac ccgcagtggt gcggcggcgc
     8581 agagctgcgg gtcgtcatcg aggcgaagaa ccgcgggaag tcgtggcgtg agatgcgcga
     8641 ggagctcaat gaggcgaagc gcaaccgcgg tgccgccgcc gccctggccg tctacacgcc
     8701 cgagcacgcg cccgccggag tcgcgccgtt cgacgttcgc tttggccacg tgttcgcggt
     8761 aatcgacccg gagtcgcctg acatggcgac gctcactgcg gcggtgcgac tggcgcgtct
     8821 ccacgcgctc gcgtgcgttg ccgtgcaaac gacggaggtc gacactgccc gcatcgccgc
     8881 ggcactggcg gcgctcaaga cggagctcga cgccgtacgc gccttaaaga tgagccttac
     8941 ggagattggc aagacggcca acaacgtgtc cgtggggctg gaccgtctgc gcgatcaggt
     9001 attggggcgc gtctcggaag cggaagctca gctcaaaccg aagtccgtct gacggtcggt
     9061 ccgagcgttg tcgcctgctc gcactcgcgt gcgtgagcga tcgaacgcag cgggcaacga
     9121 ccggatcggc cgccaggtgg gccgcctagc gggccaccat gacggggcag gacgagtggt
     9181 aaacgacgta gtccgagaca ctgccgagga ggaagcggcc ggcgcggtcg agcccacggg
     9241 tgccgacgat gaccatgtcc gcgccctcgg cttcggccgc ggcgacaatg ccctggcccg
     9301 gttcgccggt ccagagcagg aacgccgcct cgacgccgcg tgcgcgggca cgattcacga
     9361 tctgcaggag cgggccttct cgtagcgcgc gctgctggtc gacgcgtacg gtcgccagcg
     9421 gcgaaaccgg ccccccctga tcgatgacgt tcacgacgag cagccgcgcg ccaagcgagg
     9481 cggccatgct gatcgcctgg acggtcgcag cttccgaagc cggagtcagg tccgtcgcca
     9541 gcagcacggt ccggatcttg actgggctcg ggccgtgcac ctcggtctgg ggaacggtct
     9601 ccgaatcgac gctcgcaggg tggttcagcg ggagcatctc aggccccggc ttccatcaga
     9661 tcagcgcgca gcgtgtcctc ttccatcgcg gaagtctggc gggagtcctg cagcgcccga
     9721 cagtgacaat cggccctaat tggctgccga atgggctgag cgggcgactg ctagattggg
     9781 cgtgccatgg ctgcggcgga cctgtcccta ctgcccgttc acgagttcat cgtgctgctc
     9841 gaggactggc gcgacgagcc aaccgatgcc caacgggaac ggctgcgcga gtcgctcggg
     9901 cggatccgcg ccgcgtacgg tgggcgcggc gtgcggctaa agctgcacag cgcccacatg
     9961 cccgaattgg acatcgccaa tggcactttc accggttcga atgatggcga cgcgccaaca
    10021 ggcgcggcca gcgatctggc ggttgagccg atggccaggg gcgaggcgct cctatgggcc
    10081 gagggttcgg acgagtcggt cgaagccatc agcgcggcgc tgcagctggc gatcgactcc
    10141 atctggtcga agcacgaagc gcgactgcgc cggcgtcagc tcgaagcgct cgatctcgca
    10201 gtgcgcggca ttgccggcgt gcttccaccg gagcgcgtcc tccagttgat cgtcgatcgt
    10261 gtccgcgagc tggtcgatgc gcagtacgcg gccctgggca tcgtggggtc attcggtcgg
    10321 atcgagcagt tcgtgacgag cggcctgacc gacgaagttc gcgcccacat cggcaacctg
    10381 ccgcgcggcc tgggcctgct gggactgatc atccgcgagg atcgctcgtt cctcattgac
    10441 gacatcgcca ccgatgaccg gcgttacggc tttcccgaac atcacccgga gatgcactca
    10501 ttcctgggtg taccggtgcg cagcaagggc cagtcgatcg gcaacctgta cctgacgaac
    10561 aagcgcggcg gagcgccttt caatgaggcc gacctgcggc tggtcgagat gttcgcgctg
    10621 catgccggca tcgccatgga gaacgcccgc ctgcacgagg agatcggccg attggcgata
    10681 gtccaggagc gcgagcgcat cagccaggac ctgcatgact cgatcatcca gagcctctat
    10741 ggcatctcac tatcgctcga ggacctgccc gaaatcgtca gcgaggatcc ggctgagggg
    10801 accgcgcgca ccgaccacgc gattgacggc attcacgcca cgatccgcga tatccgcaac
    10861 ttcatcatgg gcctccagcc ggagctgctg atggatgcgg acctgagcac cggggtcgag
    10921 acacttgccg ccgagttccg cgcaaacacg ctcatcgacc tcgagctgca cgtcgatccg
    10981 gacctgcctg agctgccacg cgaccatgcc gcgcacattc tggccatcac ccgcgaaacg
    11041 ctgagcaaca tcgcccggca ctccagtgcg acgcgggcag cgattcagct cttcgcgaag
    11101 gacgggacgg cgacactgat cgtaggcgac aatggacgag gcttcgacgt cgaagctgca
    11161 cggagttcac gccagcgcgg gctgggcaac ctccgggcgc gtgccgaaca ggtcgggggc
    11221 cgattgatcc tcgcgagcga ggaaggcagc gggacacgcg tcacagccga gctaccgctt
    11281 aaccgagagg accaggtatg acagccgcac acgaaggcgg agagcgcaca gaaatccagc
    11341 atcggctgct ggtggtcgac gaccacgagg ttgtccgcca gggcttggtc gcgatgctcg
    11401 agcgccgagc cggcttccag gtcgtcgctg aagctgggac ggccgcggac gcgatcgaga
    11461 tggcccggcg ccaccagccc gacctcgtag tcatggatgt gcgtctgccc gacgggtccg
    11521 gtatcgaggc gtgccgtgag atccgcgcag agatgcccaa cacgcgagtc gtgatcctga
    11581 ccagctatcc ggacgaggag gccgtgttct cggcgatcgt ggccggcgcc agcggctacc
    11641 tgctcaagca ggtccgcggc cgcgacctgg tggctgcact cgaagcggtg ggtcgtggcg
    11701 aatcgctgct cgaccctgcg gtcactgaga aggtgctcga gcgggtgcgg cgcatcgcga
    11761 ccggtggcgc aaccgacgag ctggctcagc tgaccgcgca ggagcagaag atcctgctgc
    11821 tcgtagccga gggcaagacg aacaaggaga tcgcagcgga ggtcttcctg tccgacaaga
    11881 ccgtcaagaa ctatgtcagc tcgatcctgt caaagctgaa cctgcagcgg cgcgcgcagg
    11941 cggcggcgta tctcgccaag cgccacggcg gatcttccta ggcgcgacct cggcgctgca
    12001 gcggcgctgc cgctgaatcc agcgatgcct gcgcgaggcg attgatcagc gccccttgcg
    12061 cgctcgcgaa gatgtcatgc acctggcagc ggttggcgac gtcgcattgc gtgccgctca
    12121 agacgcagcg ctggcgccgg gcatcgccct cgacagcctc gacgatcgcg aggatcgagg
    12181 tcgcgccacc ctgcggcggg agccagtagc caccatggcg gccgagccgt gcgtgaacaa
    12241 gcccggcgcg taccagctgg cccatcacct gcccgacgag gcggccggga atatgcgtac
    12301 ggcgcgcaat gtccgcgccg gtcagaacat cggattcctg ggccagcagc agcatcgccc
    12361 gaatcgcgta gtcgcctcga cgagtcaact gcaatcgcat gggcatagtt cgccccatgc
    12421 cgaagggacc gagatagggc cgaatggccc atgtcagccg ccattcgaaa caacaaccat
    12481 tttggtgttc atttccatgg cggtcacagc gcctgctgca cgggagttcg caatcaaatg
    12541 aaggccacgt tccgcgcgct aatccttgtc gtcgtcgtca tcagcctgct cgcggcctgt
    12601 accaccggcc cgcggccggc gtggacgtac acgccagcga caccatcgcc gggttcgagc
    12661 gcccagccga gcggcgctcc gccaacggcc gtccccaatt cagatgacga cgtcctgccg
    12721 gcgctgaccg ccaaggccga tctgggcgca gtgcccgcgg gtgaggtcaa cgtcgcctac
    12781 gcgccggaag tgccccccgc ggcggcgcgc acgacgcagg cgatcgtcga ggtcgcattc
    12841 gatgtcgttg aaggggtcaa ggcgattggg ccaaacggac tcgaatacac gacgtggggc
    12901 tatcgcctgc acggcgacag cgccgtgacc accggcacgc ccgggcccat catccgcgcg
    12961 cgtgtcggcg atctcctccg cttctctctg accaacaacg gcacgatgcc gcacaacgtc
    13021 gactttcacg cggtcacggg ccagggcggc ggcgccaagg acacgaccgt caatccgggc
    13081 gagaccgcca tcatcgaggc gcgcctgctc taccccggga tgttcatgta ccactgcgcg
    13141 gcgggcgacg tgccgcagca catcgcacag ggcatgtacg gcggcatcct ggtcgatccg
    13201 gagacgcccc tggccccggt cgagcacgag ctgtacatgg tccagtccga gttctacacg
    13261 aacgatccgg ctgccgggcc gctgaccacc gatcgcgatg cgatcaccga cgagcacccg
    13321 cagttcgtgg tcttcaacgg cgccgttggc gccctggccg gtgacaacgc gccgaagatg
    13381 caggtcggcg agcggatgcg catctacttc gtcaacgcgg gcctcaatct cgactccaat
    13441 ttccatccca tcggctcgca ctgggacaga gtctggacgg aaggcgcgtt gctcaacgag
    13501 ccattgcgcg gctcacagac cacgctcgtt cctgccggcg gcagcaccat cgtcgagctg
    13561 atcggactgg tgcccatgac cgtcgtgctc gtcgatcatg ccctcgcgcg tgcgttcgac
    13621 aagggcgcga tcggtcagat cgtcatcacc ggcgatccga acaccgagat cttcgaatcg
    13681 atcggcaagt cgggcacccc gctgccgacg acggtgccga ccaccagccc ggtgcccagc
    13741 accacgccac agggttcacc gcttccgacc ggcacccctg gcgagggttt ccacgtggac
    13801 atcgtcgccg gtgctttcac cttccaggat gcgaatgctg ccgacgagta cgccgacacc
    13861 gagtctccag ccgactacag catcaacgta ctgatggtgc cgcttggctc aacggtgacg
    13921 tggaccaatc acgatccagg ccaggtccat accgtgacgg ccgtcgatgg atcattcgac
    13981 tcgggcttcc tgaacgaggg ccagaccttc tcgctcaggt tcgacacgcc cggcgagttc
    14041 gagtacttct gcggcccgca tccctggatg cgcgcccgga tcatggtcga gatgcactag
    14101 ggcttctggc ccccaggcag ggagtcttgg cactgtcggt tggcgctcct gcgccgctag
    14161 cctccgctct cgatctgcct tcaaagctct cgtaagcgag gggttcatac atgcgcctcg
    14221 tcaccgccgc ggaggccgtc gccggcatcc gctcaggcga acagctctac atccactcag
    14281 ccgcggcggc gccgtccgtg ctgctggacg cgctggtcgc gcgagcaagc gagctcagcg
    14341 acgtgcggat cctgcacttc cacacagaag gccccgggcc gcacctcacg cccgagatgg
    14401 cgccgcactt ccgccatcgt gcgctgttca tcgggccgaa tgcgcgcaag gcggtcaatg
    14461 agggtcgggc cgaatacatc cccgcattcc tatcggacgt tgggacgttg atcaggcgcg
    14521 gcgtcctgcc catcgacact gtgtttgtca acgtgtcgcc acccgacaag cacggcttct
    14581 gctctcttgg gacgtccgtc atcgccatgc cgacggccat ccgctacgcg aagcaggtca
    14641 tcgcccaggt caacagcgcc atgccgcgca cattgggcga tagcttcgtc cacgtggacg
    14701 acatcgacct cgcggtggag gtcgacgtgc cgccctacgc gaccgcaccc gcggtggtcg
    14761 gtgacgtgga gcgacgcatt ggtgacttcg tggccgacct cgtgcccgac ggcgcgacga
    14821 ttcagctggg catcggcgcc atccccggcg cggtggccgc agctctacag ggcaagcgcg
    14881 atcttgggat ccacaccgag atgatgaccg acgtcgtgcg cgagatggtc gaggcgggcg
    14941 tcgtcactgg ccgtgccaag gagatcaact acggccgcgt cgtggccacg ttcatgctcg
    15001 ggtcgcagcg cctctacgac tgggctgaca acaacccgat ggtcgagatg cggcccgcgg
    15061 actacaccaa tgacaccgcg gtgatccggc ggttcaagaa gatggtggcg atcaactcgg
    15121 cgatcgaggt cgacctgacc ggccaggtct gcgcagactc cattggcccg cgcatgtaca
    15181 gcggcgtcgg cggccagatg gacttcatcc gcggtgctgc cctggccgac gatggccgcg
    15241 cgatcatcgc tctccccgcg acgacaagcg acggcaccat ctcgcggatc gtgccctatc
    15301 tgcgcgaggg cgcgggcgtg gtgacgacgc gcgcccacgt cgagacgatc atcaccgagt
    15361 acggcgtggc cgagatgcac ggccgctcca tccccgagcg cgcgcggcag ctgatcgcga
    15421 tcgcccatcc cgacctgcgc ccgtggctcg aggaggaggc tcggcgggcc cattacatct
    15481 agcgagagcc cgcggaaagt gactttcggc ctccgctatc gcccgcgcat ggccgttccg
    15541 tggcgcgccc cagcgaccga cgatgcgatc ggattcagcc gtggaggctc agccgactga
    15601 agcctcccga cgaaggaggt cgcgtggagt tcgcaccagc agcccgggca ggactggcca
    15661 taacggatgc gcctgagctg ttgagcgagg tcggttggct gctggtccat ggatcgccgt
    15721 tcgagcacgg tccggcctat ctgctggtag ccatccgccc gcgcccgacg cgcgctcact
    15781 tcgatccgga acgaatcgaa tgctggtgtc ccggatccgc ggccgctgag caggcgacgc
    15841 tggagtggcc catcgcaaag ccgccgccca cgtactcgtg gggcaccatc aaggtcatcg
    15901 accgcgttgc ggcggtgaac aagttcgcgt cgttcggcgg gtcgctcagc ggggttcgtg
    15961 acgggcagct cagcgcagcg ctgttccggt ccgaagcgcc catcctggcc gtgagcggcc
    16021 gaggcgacgc cggagatccg ttggcggcca gcgtcgtggg cttcttcgca cgcctggcag
    16081 ccgcctgtgg cgcggactcc gaactctcac gtgacgtcag cgcgatggat ccgctggtcc
    16141 tctacgcggc ctacctcgta cgggcgcttg agctctacgg tggcgatctc gccgccctgg
    16201 cggcgccgcc acgactgcag gcgctgctgc gttctgagct gtgtctcttg gagcgcgact
    16261 cgggtgcttc ggccgccgcc ggcgaagcgc tcgctcgccg cctcgatggg gcgggccaag
    16321 agcggaaagg agtgcgctca tggtcggcac gattcggatg accgcagcgg acttcgcgtt
    16381 cgcgcgttac gagcatgccc agctgagggc tcagctggac gacattcgcg ccgccgccga
    16441 cacggtgggc cgcttgtcgc cgctcgaggc tgcgcgggct gtcagccgtg tgcgcgcctg
    16501 gcttgccacg actcttggcc cgcatgccgc ctgggagaac ctcgtcgtgt atccggcgat
    16561 agagcggggt accgcaacga cggcggcgac gaacgtgatg cgctcggagc accgccagat
    16621 cgaacacatg ggttttgtcc tcgaggcgga cgcggaccta ttgcgcgccg gaccggtcac
    16681 ccacgacctc gtgtgcgaca ttcgcgccca cctactcggt ctccagtcga tcctgtgtac
    16741 ccacatggag agggaagacg agttcctccg gccggaaatc gacagccctg accagaggtg
    16801 aaccagatgg acaacacggc gatgcgcgtt ctcgttgcaa ctgacggttc tgaggccgct
    16861 gatcgcgcgg tcggccttgc ctcttcgatc gaatggcccg ccgggaccaa gatccgcgtg
    16921 gtcaccgtca tcgagccggt cccaccggtg cccgcggcgg agtgggtcgg cgcggccgcc
    16981 cccgttgaag cgatgtaccc gcacgaggcc gacagggtcg ccaaggcaat cctcgagcgc
    17041 gcctgcgatg cgcttgccca cacgtcggcc gaagtcagcg ggcagatgct gtacggccgc
    17101 ccggcgacca gcatcctcga gagcgcgcgc gagcttggtg cggacctggc gattgtcggc
    17161 agccgcggtc acggcaccat cgcgtcgatg gtcctcggct cggtcggcgc tgaggttgcc
    17221 gacctggccg aatgccccgt tctcatcgcc cgcgggcgcc acctgacgcg ggccgtcctc
    17281 ggcgtggacg attcggagtt ctcgcgcgcc gccgagaagg tcgtggccga gtggcccatc
    17341 ttcgccaagg tggcgatcga ggtcacccac gtcgcccagg tagcgctgcc gtggatggtc
    17401 gccctggggc cgggggtgcc ggtgcagccc gagattgggc cagatgagta cgagacggtc
    17461 ggggccgcca cacggcggct caaagaggcg ggtcgccatg cctttgggcg gatggtcgtg
    17521 ggcgaccccg cagccggcct gctgctgacc gcagaagagg cgcaggccga tctgatcgtt
    17581 gtcggcacgc atggccgaac ggggctcagt cgcgcggtct tcggcagcgt cgcgcgcaac
    17641 gtgatgctgc acgcgccgtg ctcggtcctg gtcgtgcgca ggatccggga aggccagaca
    17701 cagctcaagg cggcgtaggt cggccgctca caccagggcg tcgtgttcgg cgcgagcgga
    17761 cgagggccga ttggcccgcc atttcggtac cgcgcgtcac gtgtcagtcg gcccgctgcg
    17821 cgccaccctt gcggtgtgaa tgctgcgcac ccggcctatc cgggcatacc cgcgatcatc
    17881 gacggctcgg aggctgtggc ccacgtcgag acgcgcctcg ccgaagcctg ctgcgcgtac
    17941 ccaatcacgc cctcgaccaa catggccacg ctcttccagg cggcggtggc ggatggcaag
    18001 accaacctgt gggggacgcg gctgtccttc ctcgagcctg agtccgagca ctccagtgcg
    18061 tccgctgcgg agggcttcgc gctcgccggt ggacgggtga ccaacttcac cgccggccag
    18121 ggcctgctgc tcatgaagga ggtcctcttc acgatcagcg gcaagcgcct gccggccgtg
    18181 ttccatgtcg gcgcacgtgc cctgaccagc cagtcgctca acatccatgc cggccacgac
    18241 gacgtgatgt cagtggccga cgctggctgg ggcatcctct tcgcgcgcaa cgtccaggaa
    18301 gcggcggacc tgacggcgat cgcgcgtcgt gttgccgagg agacggagac gcccttcctg
    18361 gtagtccagg acggctttct gacgacgcac accctcgaga acgcgctctt gccggaagac
    18421 gagctgctgc gcgagttcgt cggcgaccca cggctgcgca tccgcaacct cttcgagccc
    18481 tccgaggcac tcatgacggg cgtcgtacag aaccaggact cgtacatgaa gggccgcgtc
    18541 gcccagcgcg cctggtacga gcgcattccc gcggtgatgg acgcggtcat gaacgagtgg
    18601 gccgagctga cgggccggcg ctacggcgcg atcgacgcct acctgaccag ggaagcggag
    18661 cacgtcgtgg tcagcatggg cacgatcgcc gatagcgccc gcgacgtcgt cgaccacctg
    18721 cgcgccactg gtgtgcgcgc cgggggcgtg accgtgacat cgttccggcc tttcccggcg
    18781 actcagatag cggccgcgct ggcggacgcc aggtccgtcg cgatcgtcga gagaaccgac
    18841 gagccggcgg cggcggccaa tcccctgacg cgtgagacga agtctgcgtt ggcggatctg
    18901 gcagctgccg gacgatccat cccgcgcgtc gtctccgcct ctgccggtct cggcagccgc
    18961 gacgtggcgt cggccgacct gctggcggtc ttcgactggc tgcgcgatcc cagcgcgatg
    19021 gacgagcggc cgtacgtggt gctcggcatc aagcatcggc tggggctcgc cgcccccgac
    19081 atcgagctgc gcccgaccgg cgcgtacagc ctgcgtggcc attcgatcgg cggctacggg
    19141 tcggtcacgg tcaacaagct tctggcgtcg atcgtcggcc agacgttcgg cctgtacgtg
    19201 caggcttacc cgcgctacgg ctcagagaag cgcggcctgc cgacgaccta ttacctcacc
    19261 accgccgagt cgcccatccg ccagcacagt gagctgcgcc gggtcgaatt cgtgccgctc
    19321 cacgacgtcg cggcgttcgg ccagggcgct cccctgtcag gcctggtcga cggtggcacg
    19381 ctgttcgtga acagtacgct cgacgatccg gccggcatct gggccgcact gccggcgaat
    19441 gcgcgcgcgg agatcatcgg ccgcggcatc aacgtggtcg cactcgacac gctcgcgctc
    19501 gcgaagcgct tcgcgcccac gcccaacctc gcgacgcgca tgcagggcgc cgcgctggtc
    19561 ggcgtgttcc tgcgcgtcgc acccttcatc gcgggtcgcg gcctgtcact cgacgacctg
    19621 atggccgccg tcaaggggcc gctcgccaag catgttggca agcgcggcgg cgcggtcgtg
    19681 gacgcaaacc tcgctctaat tcgcgcggcc tacgaagggc tgattgatgt aaccgcgccg
    19741 ctgcgcgccg cgccgcccca ggcggccggt gagggggcgc gagagatggc aggagtcacc
    19801 cgatgagcgc cgagctcgag gtcgtccacg acgaggccga cgtgcccctg gtgggccagc
    19861 tcatgacacc cgaccccatc gtggtcgagg acggggcgtc actcgatgaa gccgtgcggc
    19921 tgctcgagga gaacgagatc agcggcctgc cggtggtcga tcgcgacggc ctgctggtcg
    19981 gcgtgctgag ccagaccgac atcatccacg cgcgcgcggt cgcgcacctg tggagccgct
    20041 ggcctggcct gcgcgtgcgt cacctgatgc atgccccggc gctgaccgcc gaccgcgaga
    20101 tgacgatgga ggaagcggcg cagatcatgg agcgctccca cgtccaccgt ctgatcgtcg
    20161 tcgacgagga tcagctcaag ccggtcggcg tgatctcaac gacagatctc gttcgcgcac
    20221 tggcgcacag gccgaccgat ggttgagctc gacaccgcgc gtcctgcggt cgacctggtc
    20281 gactacgacc gcgcactccc agtcttcgac gtgggctcgt atgtcgagga attcaacgag
    20341 agcgtcatgc ccgcgtacaa gagcggcacg gccgatcgcg agtacgccgc cgacaacggc
    20401 ctggcgcgga gcatcattcc ccccggcacc gctgcgacgc gcgacttctc aacgctcgcg
    20461 tcacggattc cgcagctcct tgcggacgcg tgcgtgggct gcatggcgtg cgtcagtgcg
    20521 tgtccggaca ccgccatcct gggcaccgcc cagccgcgtg cagcattgaa ggagcgcatc
    20581 gccgagttca gcgcggccaa tgccaacggc gtggccgctg acgtgatcgc gctcgacctg
    20641 acagcccaat ttgtcgcaac ccagaagtac gcgaccgtgc ccgagcggcg cggcctcgag
    20701 ccggcagact tcgggatctt catcgacccg acgaactgca agggctgcgc cgagtgcgtc
    20761 gaggtctgcg cccacctcga ccacaacgcg ctggtgatga tcgacaagga ggcaccgacc
    20821 gaccgctcgg aaggcacgct tgagccgtac cgccgccgga tgagcttctt ccgatcgctg
    20881 ccgacgacgc cggtcgagta tcggaatgag aaggcgttgg ccgacctcat gctcggcgag
    20941 cacgccttgg gctacgtggg cggcgcgggc tcgtgctcag gctgcggcga ggcaacggcc
    21001 ctgcggatga tggtcgccgc gacgcgccag gtgcacggcc ccgagtccat gggcatcgtt
    21061 gccgcgacgg gctgcaacac cgtctatggc agcacctatc cgttcaaccc gtacatggtg
    21121 ccgtggacca actcgctctt cgagaacgcg gctgccgatg caatgggcat cagggcgcgc
    21181 tgggatcagg ccggccacac cgaccgccgg ctgtgggccg tcggaggcga cggcgcgatg
    21241 tacgacatcg gcttccaggc gctctcacgc cttgtcgcct cgggcgcgga cgtgaaggtg
    21301 ctggtgctcg atacccaggc ttattccaac accggcggcc aatcatccac cgcgacgttt
    21361 ggcggccagg tgacgaagct ctctgcattc ggcaaggaga tccacggcta tccggcgcgg
    21421 cgcaaggagc tgggccgcat catgctcgcc catgaccagg tgttcgtcgc ccagaccacc
    21481 acggcgcatc tcaaccactt ctaccgcgcg gtcattgacg cgaacgagtt tcctggcccc
    21541 gcagtgatcg tcgtctacac gccctgccag ccggagcacg gcatcggcga tgaggcggcc
    21601 gctcagcagg ctcgcttcgc cgtggactcg cgcgcgttcc cactcttcag ctacgaccca
    21661 cgccgtggcc cgtcactggc cgagcgtctg tcgctgcagg gcaacccggc cttgcgcgag
    21721 gactggctgc acacgcccga tggcacggtc accgacttcc tcgcctttgc gaggacggag
    21781 ggccgcttcg cgccacactt cgcggccgac ggcagtccaa gcatcgagat ccaggcgacg
    21841 aacgccgatc ggctcgagaa ttggcgactg ctccaggaga tggccggact gaggaaagag
    21901 ggggcagctc aataaccact gtcacaggga gtcaacagtg cacgtgagac gcgggttcct
    21961 gaactggggg atcttcctgg tctgcctggg cgcggtaccg ctggccgtcc agctcaacgt
    22021 gatcgatcgc gacgtggcca cgtcgttgct gcgactgtgg ccgctcatcc tgatcggcat
    22081 tgggctgggc ataatgctgc gcttcagccg cgccgcggcg ctgggcggca tcgtcgttgc
    22141 cgcgaccttc ggc
//
LOCUS       DATBRX010000131        57482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2276346, whole genome shotgun sequence.
ACCESSION   DATBRX010000131 DATBRX010000000
VERSION     DATBRX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 57482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57482
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276346"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VIF08_04810"
     CDS             complement(<1..1022)
                     /locus_tag="VIF08_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135341.1"
                     /translation="MPNLLSRFLDANGREVRKLQPVVDEINSLEAEFQALSDAEIRAR
                     MIELREQIHEDGAPSEPSDEELNADSSERRRELRRARETADVKHLQGVLDDALPEVFA
                     AAREISRRKLGMRHFDVQLIGGVVLHQGKIAEMKTGEGKTLVAPLAAALNAMSGRGVH
                     VVTVNDYLAKRDPQWMGPIFDGMGLTVGVIQHETAYLYDPEHRGTDERLLNLRPVERR
                     EAYAADVTYGTNNEFGFDYLRDNMVTELNQRVQRGRYFGIVDEVDNILIDEARTPLII
                     SGQAAESEDLYYQFARLVPRLQKRPEGAEEGGDFFVDLKEKAVAPTEEGISKIERLLK
                     IDNLFDT"
     gene            complement(1070..1678)
                     /locus_tag="VIF08_04815"
     CDS             complement(1070..1678)
                     /locus_tag="VIF08_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8135342.1"
                     /translation="MDSGSRRQRSFRQDGGSALDDLIFRLRARRILRQFPPYAAVAAD
                     LGCGHDYRLLRHLHRSRRIGAGIAVDLALGQPPPPGITLIETDLGRPLEIASASVSVV
                     MSLAVLEHLDDPGLHLREAYRVLRSPGLLLLTSPSRASRPLLEFMAYRLHIIDAEEIR
                     DHRHYFNEAEIRSLLVDSGFSASTVEYRTFLLGLNQFASAAK"
     gene            complement(1691..2698)
                     /locus_tag="VIF08_04820"
     CDS             complement(1691..2698)
                     /locus_tag="VIF08_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8135343.1"
                     /translation="MTLVQTRPPAIRSTGRGIAVIIPCLNEAQTIGQVIDDFRRELPE
                     SRIWVIDNDSTDATAKVALEHGAQVLTETRRGKGFAVRTAFRSIDAEAYVMVDGDDTY
                     PAEAVHALLAPISTGRADMTVGSRTMEGTNSQFHFLNRFGNRMYAALVRFLLRVRLTD
                     ILSGLRVMSRQLVRTVPIAASGFEIEAELTVKTIERSMRIVELPIDLRSRPKGSASKI
                     KVFRDGARIAWTILLLFRDYKPLTFFGGIGLVFMAIGLIPGIEVVLEFARTGLVLRLP
                     SAVLAAVLEVVGILLGVVGMVLSSISRRFQEIESKLDLIAHEQIDALREPVDQVGGQG
                     G"
     gene            2853..4439
                     /locus_tag="VIF08_04825"
     CDS             2853..4439
                     /locus_tag="VIF08_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135344.1"
                     /translation="MSSTSVTMAAETRSTLVVRNERARVFGASLAVFIFTFLVYYLTG
                     PVNTPYDFQLSQANNIIHGHLDMTAEYTRNLNVLERVLYDGQGFCLPINDPRGPEIDA
                     LIPNARFSADCKHYMQHSLGPAFLLLPLVLIFGMTVSQALVSALIGAAGAVVVFAITR
                     HFTSSLRTQLALTVLGVLGTQIWFSSADGSVWHFAHATAVFFVFSGIYFTVVRRNPLL
                     AGAMFGAAFMCRPSTIIGGLFALVAFSDMWLPAATEAGRSILRRIQLRPLVALAIGVA
                     PFLVLNSLVNYLRFGSPFESGYSYTEQLYQDNLTWVYPHGLFSPQYWSRHVAIVFEQM
                     PNFSGNPPFIWPSWAGQAMWATSPALLLGLFVHLRKWVWPARLAAIAIALSGAVLISG
                     AAGAQLGVVRWTPASVPFALHLWPFWIVIGLAIVLSITNRDRLVVACWAAIVPIVLFI
                     WMFAATGWAQFGYRYGLDFVPFLFLLAVIMVTRGMRLTRYQVVLIGASVLINLWGVLW
                     ILKFAIAPGGPLNGWTWVSY"
     gene            complement(4486..4722)
                     /locus_tag="VIF08_04830"
     CDS             complement(4486..4722)
                     /locus_tag="VIF08_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135345.1"
                     /translation="MSLKLYRPTPNGLEPSPVEQRTWRSRLRSRRWKPAELANPEQAP
                     TSTRMAVAFWVVLAVLTFVVLVVGYGTGFWGSIS"
     gene            complement(4738..5835)
                     /locus_tag="VIF08_04835"
     CDS             complement(4738..5835)
                     /locus_tag="VIF08_04835"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY8135346.1"
                     /translation="MSAFGGRAATVYSDQYDLYTGNSNPELARKIARYLGRELGHAQV
                     FEFPNENIFVKIEDNARDRDVFIVQSTNSPVQKSIMELLIMIDAFKRASAGRITAVIP
                     YYAYGRSDKKDQPRVPITARLVADMISVAGADRVLTMDLHQGQIQGFFSIPVDELTAV
                     HMLSSHFKKLNLEDPVVVTDLGFAKRARTFAEILDAPLAVIEKRRPDNRGTAELLNVI
                     GEVKGKRAIIVDDEIDTASTLMEIERALIREGVTDIYACATHGVLSDPATDRLRASEI
                     TEVVITDTVPLAAAKQLPLIKVLSVAPLIGEAIRRIHRGESVGALFSSDMSLVQEMIL
                     WDEETPRGEDDDTFRDGVGHGEPAPAAAHAG"
     gene            complement(5832..8396)
                     /gene="gyrA"
                     /locus_tag="VIF08_04840"
     CDS             complement(5832..8396)
                     /gene="gyrA"
                     /locus_tag="VIF08_04840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY8135347.1"
                     /translation="MTDEGRVVPIRIEDELRVSYLDYAMSVIVSRALPDVRDGLKPVH
                     RRILYTMHEMGLTSAASYRKCAAIVGEVMGKYHPHGDVALYDALVRLAQTFSLRYPLI
                     DGQGNFGSVDGDPPAAMRYTEARMTAIAAEVLADIDKQTVDFVENYDGTRQQPSVLPA
                     KLPNLLVNGSAGIAVGMATNIPPHNLGEVAEATIALIENPSLTNDELCGIVKGPDFPT
                     GATIFRRDTRRNSVTGQVEEFDAIREMYAHGRGRIVMQATVSFEETRQQRMAIVVTEL
                     PFQVNKATLAEKIADLVQHKKVEGIADIRDESDRDGMRLVIECKRDAAPRKVLNNLFK
                     HTALKLAYNVNMLALVDGQPQTLPLKGVLQHHIDWRREVVRRRTEFDLGRARDRAHIL
                     EGLKIALDHLDAVIKTIRESADVDTARANLMSRFELTEIQANAILEMQLRRLAALERK
                     KIEDEYLAMIKLIAELEDILANPARVLSIIKDELREMSAKYSGERKTRVQEDADRELT
                     DEDLIADEDVVVTVSKRGYIKRQPLATYRRQIRGGKGIIGARTVEEDAVEHLLVAGTH
                     DWVLFFTNQGRVFSSKVHAIPAPTERTSKGIPVVNLEGVQLEPPNERVLAIITIPNFE
                     KGDNLVMATRKGMIKKTPIEQFERVRSTGIRAITVADKDELAWVAVSSGSDDILLASA
                     QGKIGRFNEKEVRPMGRDAAGVIGIRLARAGDQVVNMGVIKPGNDVLVLTETGYGKRV
                     EVSEFRRKHRGGQGVMLIPIEGSKTGKVAAVQLVDEQDEELLLISREGQVVRTDVKSV
                     NRYGSGARGVIVMRLNEGDIVAGIAVFRAETRDTVEPGLADGEPMPDNDGPAGGSS"
     gene            complement(8393..9382)
                     /locus_tag="VIF08_04845"
     CDS             complement(8393..9382)
                     /locus_tag="VIF08_04845"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY8135348.1"
                     /translation="MTSDITRRAQALVAERLPEARGLGQTLAELIDQPEEFVAIARTG
                     LTNLADEPYRAEQARVAPGTGAVFGVRNPLLAAIARQLKASLKQGSDASALWLAERLV
                     IEPEREFTILAHTCLLRSLPSDPERTWQLMRKLAHAAHEWISVDSLAGLYAAGIMREH
                     YRWAELEQLVYAASPWERRLVGATLAQLPFELGRGERPRLARTPALTLIRTLLGDAEP
                     DVQKALSWALRSWFEVDPNGVRELIRTEAGDALRNNDGHRAWVLRDALTSPLVDGSFA
                     AEIRARLEGVRRDGRKPSTSEASETARGFVGYERLADAALEQQGARQQGRGQR"
     gene            complement(9372..11345)
                     /gene="gyrB"
                     /locus_tag="VIF08_04850"
     CDS             complement(9372..11345)
                     /gene="gyrB"
                     /locus_tag="VIF08_04850"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEY8135349.1"
                     /translation="MTDQLTKPTARGSAGNGRKAKGAADAAYTAESIQVLEGLEAVRK
                     RPGMYIGSTDGRGLHHLIWEVVDNSIDEAMAGHATLIELTIKPDGSVVNRDNGRGVPV
                     GKQKQTGKDALEVVHTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSEWLYVESAFGE
                     KVWRQDYERGKPTNSVHAVRASNGSHGTLTAFKPDALVFETLEWSFETVEQRLRESAY
                     LNKGLWIKFVDERPTPAREKSFYFEGGLVSFVRHLNRDRDVLHPRPIYAERRDGSTQV
                     EVAIQYNDGYSESVFAFANNINTVDGGTHVTGFRAALTSSLNEYARKNAILKDSDANL
                     SGDDVREGLTAVISVKLTDPQFEGQTKAKLGNAEVKGQVQAAVAEGINQYLEENPQDA
                     RRIIEKSLLAARARDAARKARDLVIRKSALEGSALPGKLADCQERDPARSELFIVEGD
                     SAGGSAKQGRDRHFQAVLPMFGKMLNVEKARIDKVLSSEKIRPLIIALGAGIGETFDI
                     AKLRYHKIILLADADVDGAHIRTLLMTFFYRHMPFVIEGGHLYIAQPPLFRVSTGKVT
                     RYAKDEADRERVVKEFKAKNLTIQRFKGLGEMNAEQLWETTMSPATRTLLRADIVDAA
                     EADRIFSTLMGEKVEPRRDFIKARARQVQNLDV"
     gene            complement(11342..12202)
                     /locus_tag="VIF08_04855"
     CDS             complement(11342..12202)
                     /locus_tag="VIF08_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8135350.1"
                     /translation="MTAEGRRSGGLGRGLAALIPQRDEPAASVELPISAIRHNPYQPR
                     KGIEQANVADLAASIAEHGVLQPILVTHSADGYQLIAGERRLRAAQMAGLERIPALVR
                     SADGGAQLAWALIENLQRSDLNALEEAHAFRRLVDEFGLSHEDVGARVGRSRSAVANT
                     LRLLDLDEGVQAALTAGQITEGHARALAGLPDAAEQANALSQVLSSGLSVRQTEELVR
                     RSIKGKPKSTRSATARPADVERLERGLREALATKVTLSTSRKGGKITIEYYTQEELER
                     LYERLTGGAA"
     gene            complement(12199..12993)
                     /locus_tag="VIF08_04860"
     CDS             complement(12199..12993)
                     /locus_tag="VIF08_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8135351.1"
                     /translation="MGDVIACANQKGGVGKTTTVINLAAYLALAGRRVLVVDLDPQGN
                     ATSGLGVDRQGGASSIYPALVDPQLTRSVVQPTQIDGLSLIPSGLDLAGAEVELVSVG
                     QRERKLSAALAQVRDAYDFIILDCPPAVGLLTVNALTAADAVLVPIQCEYYALEGLGQ
                     LLATVDLVRDNLNPRLRLGGVLLTMYDARTTLSSDVAAEVRRHLNGTVYETIVPRSVR
                     LAEAPSYGRPIVRYSPESRGAQAYAALAEEVLQRHGARGPVAGVGA"
     gene            13276..13776
                     /locus_tag="VIF08_04865"
     CDS             13276..13776
                     /locus_tag="VIF08_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135352.1"
                     /translation="MTIPFAALFTILAALLETTVLPELPIAGAIADLVLICATCAAII
                     LGIEDGLAAAFLGGLMIDLLVPDRPLGAATLSLLLVLSLAMIVARVGGPSRRWLAVAM
                     TVILTPLLHGLMTVILVATEGVPLVFSPSAVLVAAFMNGVIAIPVATLFTAIEHRFGT
                     VERVDW"
     gene            13780..15690
                     /gene="mrdA"
                     /locus_tag="VIF08_04870"
     CDS             13780..15690
                     /gene="mrdA"
                     /locus_tag="VIF08_04870"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8135353.1"
                     /translation="MADYVLGRPEPPQNRLGVRFGVFGLIVVLVIGALTTRLFYMQGV
                     QGGYYTDLAEANQHRTLPIKSARGIIYDRAGRELAINIPSYVVYIRPADLPFTQRDAV
                     ADELGRLLGIDPTEIIAEVDRYASQRFELVRIASDISSDVARIIVEENRNLPGVEIGI
                     EDRREYEYGPLLAHVLGYVSPISADQLAQLKDEGYLADDRIGQTGVEARFEDVLRGTY
                     GQEQVERDASGRIVRTIQILEQPQAGNSLELTIDTEIQREATEALQWATDIVNLQHGV
                     IIVANPQTGEILAMVSLPTYDDNMFARGITTAEYKSLIDNPNHPLVNYAISEQYPPGS
                     TYKLVTASGALMDGLLSPTQQLETAAYLEIGPYRYYDWNRRGFGPLNVTQAYAISSDT
                     YFYQVAGMLGIDRLAYWADQWGFGKRTGIDLPAEAKGIIPTNAWKEATFGQEIFPGEV
                     YHAGIGQGYDAVTPLQLLNAYCALANGGTLYKPQIVRRVLAPDGTVVQDFQPEVLSTV
                     GIDPEILRTMRLAAREVVTTRHSGNLVDLPLVVAGKTGTAEFGTRDGEGRLPFHSWFV
                     AFVPNFTADQAGDPAATDSELAIVAFAYDAVVKANAATEMVKYFLQEHYDLGVDLTRP
                     DLMQGGNLWGGN"
     gene            15700..16845
                     /gene="rodA"
                     /locus_tag="VIF08_04875"
     CDS             15700..16845
                     /gene="rodA"
                     /locus_tag="VIF08_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY8135354.1"
                     /translation="MRIERSQLSTLGARPESGSWRSFDIQLAVAALSLAVIGLLMAWT
                     NSPDGPLSVGSVFTRGLMWFAIAIIAFAAVAVFDYRWLSTFAAPIYFINIGLLILTLI
                     IGSTINGAQRWLSIGGLTFQISEISKVVMIGVLATFLEARKNNLGGLSTLIGAGLLIA
                     PPFVLVMIQPDLGSALVLVAIAAGALFLSGASLRWMGLAAASVVAAVPVIWSILQDYQ
                     RRRLLSFLDPTSDPQGAGFQVIQAQIAVGSGGLFGKGLTNGSHSASDLLPVQSTDFAF
                     AVLLEELGFFGGVVVFLLFIWLIWRVLLVGWRSGSVFGIAFAGGVASMIVFQILVNAG
                     MIAGIMPVTGIPLPFITHGGASLVSTAIALGLMQSIAMRSEQPRPSW"
     gene            complement(16892..17347)
                     /locus_tag="VIF08_04880"
     CDS             complement(16892..17347)
                     /locus_tag="VIF08_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY8135355.1"
                     /translation="MNPDPRTTHYKVLMLAESADQEIISTVYRKLAQRYHPDVDSSHE
                     AAQRMAAINEAYQTLRDPQKRRRYDEWLAARRDRRASDRLVRHASDVATGGAGPAVGP
                     PSGSLIDFGRYSGWTLGQIKRRDPEFLEWLMRAPAGRMYRAEIEALLRR"
     gene            17565..18206
                     /gene="rplU"
                     /locus_tag="VIF08_04885"
     CDS             17565..18206
                     /gene="rplU"
                     /locus_tag="VIF08_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY8135356.1"
                     /translation="MYAVIETGGKQYRVELGSEFAVERLEGTPGDTIKFERVLLVADG
                     DKASIGTPVVDEALVTASLVRQDRGEKIIVFKYRPKARHRSKNGHRQEQTIVRIADIT
                     LGGRSAATEAEAEQKEASKAKAKAAAEAEKKAAADQALAAKLEKDAQAKAADAKAAAA
                     KADKPAETKATKAKSEPKAKAAATKASAPKAAKPAAKKTETAPAKKPRAKKDE"
     gene            18212..18508
                     /gene="rpmA"
                     /locus_tag="VIF08_04890"
     CDS             18212..18508
                     /gene="rpmA"
                     /locus_tag="VIF08_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY8135357.1"
                     /translation="MAHKKAGSSSKNGRDSVGQRLGVKAGDGQLVSAGSIIVRQRGMT
                     FLAGPGAGLGKDYTVFALTDGRVKFEHEKRDKKRVRIVAETAQTAQTAQAGAKA"
     gene            18505..18717
                     /gene="rpmE"
                     /locus_tag="VIF08_04895"
     CDS             18505..18717
                     /gene="rpmE"
                     /locus_tag="VIF08_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY8135358.1"
                     /translation="MKPDIHPKYHAAKVVCASCHTEFEVGSTQQTLHVDICSNCHPFF
                     TGKQTILDRAGQVERFQKRLERTARL"
     gene            18759..19724
                     /locus_tag="VIF08_04900"
     CDS             18759..19724
                     /locus_tag="VIF08_04900"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEY8135359.1"
                     /translation="MARTDFGGQALLEGVLMRGRNSIGIALRHPDGSIVSTAEPLNSI
                     LHRNRFARAPFFRGLVLLYETLVIGTRWLMRSGSVQAEGEGQELGKGTIALTLIVTLG
                     MAIGLFVLLPLFLAGAATNAVAGPGQTLLAHLVEGLIRVVIFVGYLLLVSRFGDIQRT
                     FQYHGAEHMTIHALEHEDPLTVEQVRKYPTAHQRCGTEFLVIFIILSILMFSLLAGQS
                     LVVSIVGRVLLVPVIASLAYELLRFGARYRHHWWIRWLYLPGIWLQGITTRQPDDGMI
                     EVAIASMREALAANGEEAPAGSLDPLRRPIPSGVEETAGSANQAS"
     gene            19724..20812
                     /gene="prfA"
                     /locus_tag="VIF08_04905"
     CDS             19724..20812
                     /gene="prfA"
                     /locus_tag="VIF08_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY8135360.1"
                     /translation="MGLDERLDALERRLMGIESEWARPEVASDPAQRRALGREQSQIE
                     PIVHTYRRLRLVREQLASAHAAESDPELRDLARAEIDQAEIEERVLTEELRVLLLPRD
                     PNDDKNVIVEIRAGTGGEEAALFAAQLFRMYKRYAERHRWQSELISANETGIGGLREV
                     IFEVRGQGAYSRLKFEGGVHRVQRVPETESSGRIHTSTVTVAVLPEADEVEVQIDDKD
                     LRIDVYRSQGPGGQSVNTTDSAVRITHIPSGIVVAIQDEKSQHKNRAKAMSVLRARLL
                     EQEQRKAHEAEAEARRSMVGSGERSEKIRTYNFPQDRVTDHRIGVDLHNLPNVLDGDL
                     DRLLDELISTDQAQRLAHVEEASTAGVA"
     gene            20833..23046
                     /locus_tag="VIF08_04910"
     CDS             20833..23046
                     /locus_tag="VIF08_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY8135361.1"
                     /translation="MAKTVSAENAPRDIFEEPLAPGAPAPPVVRATDLTGVHVLKAGD
                     MFMLNNAHGDVRPDGRGLGLFLGDTRFLSTYDLVLNGTHPVVLRAGPAAAQHSVIQMA
                     NPDLLEQGGDVSEEVVLRRHSLGVVRDRTVSDRLKERVTVTNYTTLPQRARLTLMLDA
                     DYADIFELRGLVRERRGDRLANEWSPDGLTFTYRGLDRIVRRTVISLEPHMHRAATTV
                     PDAELDTAGTTLELDWLIEPGRSETLNIEVRAEIRDDSPPHTPPRPWTVGATIETNHV
                     LAERALERSRADLNLLINDGPAADERYIAAGVPWFSCLFGRDSIITSLQLLAVRPEIA
                     ATTLSVLARLQATEVDEFRDAAPGKILHELRTGELARTDEIPHTPYYGTVDATPLWLM
                     LLDEYERWTGDSALVDQLWPNALAALGWLDRYGDLDGDGFIEYQRQSERGLVNQGWKD
                     SADSNRNRDGTLAEGPIALVEVQAYAFAARRGMARLARRRGDEELAALQDAAAEALRQ
                     RFEKAFWMEDVGTYAIALDGAKRQVTGIASNAGHALWTGIADPQRAARTAEVLTGRGM
                     WSGWGIRTLSAETIGYNPIGYHLGTIWPHDNAICAAGFARYGLSEETRLVAGALFEAT
                     AHFREARLPELFCGFDRATSPLPVPYPVACSPQAWAAGSLYHLMSATLGFRPDARAHR
                     LELVRPALPTWLPSLRVRGLRVGDAHVDLEFGAQEQSISVEVLRRTADLDVVVRL"
     gene            23043..23942
                     /gene="prmC"
                     /locus_tag="VIF08_04915"
     CDS             23043..23942
                     /gene="prmC"
                     /locus_tag="VIF08_04915"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8135362.1"
                     /translation="MTTTVGTLLREAGQKLTEAGSETPRLDAELLLGHVLGVGRATLL
                     AEPESQVSQAQSAVFAGLIERRARGEPVAYLRGLKEFYGLALSVDERALIPRPETETV
                     VERALALIAERLTSSPRPADAQPLLVWDVGTGSGAIPVALAVECRRRGYGRDVAFRAT
                     DVSADALALATENAVAHGVADRIAFAVADLADLPDAEPVDILTANLPYIRSDDLPQLP
                     IAASYEPTVSLDGGVDGLTLVRQMVAQLPGVVVPGGVALLEIGSDQADALAALAPTGW
                     EVSVHDDLAAWPRVIELTRPASA"
     gene            23942..24583
                     /locus_tag="VIF08_04920"
     CDS             23942..24583
                     /locus_tag="VIF08_04920"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8135363.1"
                     /translation="MEVVRSDEPGALERAAGALGRGEVIAIPTETVYGLAALPRAGSV
                     EHLIAAKQRSTEKGIQLLVDSLEQVRAVCMVPPAAQKLADAFWPGGLTIVLDRRADSD
                     LPLLLGGGRATLGVRLPDHPLPRELARMLGPLAASSANVTGRPPATTAHMVIESLSDV
                     VSLIVDDGPVRGGVSSTVVDCSGSAAQAPVVLREGAIAGSEIFAALGQEYTSR"
     gene            24607..25920
                     /gene="glyA"
                     /locus_tag="VIF08_04925"
     CDS             24607..25920
                     /gene="glyA"
                     /locus_tag="VIF08_04925"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY8135364.1"
                     /translation="MRTRPDLEAVSLSVSTTSRIAWAPLAEVDPDLWGAMQRERERQR
                     WNIELIASENYAFAAVLEAQGSWLTNKYAEGQPGKRYYGGCEFVDEVEELAEQRALAL
                     FPGAEHVNVQPHSGAQANMAAYVSVLEMGDRVLGMNLAHGGHLTHGSPVNFSGKWFEI
                     HAYGVREDDARIDYDALEKQAREVKPKLVVAGASAYPRVIDFERIAQIAHGVDALFFV
                     DMAHIAGLVAAGQHPSPFPHADLVTTTTHKTLRGPRGGLVFCRADLAKQVDKTVFPGL
                     QGGPLMHVIAAKAVALHLASTDEFRSDMKHTVENAKLLATTLADNGAAVVSGGTDNHL
                     MLVDVTPLGVTGREAETLLDEIGITVNKNAIPFDKNPPNIASGIRVGTPATTTRGFGP
                     DEMREIGQLIVKAIQGRNDESASQLIKTSVREMCARFPVPGLPAE"
     gene            25917..27026
                     /locus_tag="VIF08_04930"
     CDS             25917..27026
                     /locus_tag="VIF08_04930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY8135365.1"
                     /translation="MSPDALGALPYVIAALIATTVASLLLTPVAIRLAPRLGAIDVPD
                     SERRIHSRPIPRTGGLAVALSFLGVGALGLLANQVFHLALPGRPVRASELAALFIGVA
                     IGAGFGYLDDRLQLKARWQFIAQFVLAGIALAGGVLINRLPEPLGPITDAAAMAATTI
                     WIVGMLNSVNFVDGLDGLLAGISLIAVTTLGINSLFAVSIQPVVAVLCALLAGALLGF
                     LPYNFHPARVFIGTSGVFAVGYALAVLSVLGTAKVALALLVLGVPIIDTFWIILRRVS
                     AGRSPFSADRGHFHHRLLDLGLSHRQAVLLIYLICSILAVLSLALPSGGQITVFVLVV
                     LGGGALLYLLTRRAREALDRSSYEDEGPGASSGNE"
     gene            27064..27285
                     /locus_tag="VIF08_04935"
     CDS             27064..27285
                     /locus_tag="VIF08_04935"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY8135366.1"
                     /translation="MNDLGRATAYLALFSEIAIVLLVTTLLGVLSGYWLDQRLGTLPI
                     LSVAGMLAGFGLGGVGVYRLIVRFLARFE"
     gene            27311..28420
                     /gene="atpB"
                     /locus_tag="VIF08_04940"
     CDS             27311..28420
                     /gene="atpB"
                     /locus_tag="VIF08_04940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY8135367.1"
                     /translation="MTDTPPHKGAMTPADKPTEAEARMREALGPEMASDAVGAAAAVA
                     SKPRSNTRRWIFLAIVAIVVLQVGAAIFFPPTGPDGKPYQYPADGMIGANIELPAPEV
                     VFDPTPETHRDTGLVTFDITVSNTLFTGWIVMILIVAVAFLMSRAVKVVPTRAQNALE
                     YVYEGLANFATSLGGRQARRYVPIFAAFFLFILLSNWSGLVPPVGRVHWLRAPTSDVN
                     TTIGLALFSFGMFHVEGIRALGLRRYLGKFFSLRAFRTEGVSAGLIALFVGIIEFLLE
                     FIKPVTLAMRLFGNIYGGEVALGVITGLTIALIPAAMVGLEFLLNFMQALIFSVLTLM
                     FTIIATEAHDDHAEHEAPEGNIAPDMAGAHPAPAH"
     gene            28479..28691
                     /gene="atpE"
                     /locus_tag="VIF08_04945"
     CDS             28479..28691
                     /gene="atpE"
                     /locus_tag="VIF08_04945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY8135368.1"
                     /translation="MELIGAGLAALGVIGPGIGVGILTGLSTSAIGRNPDAAAQIRAT
                     FIIGAAFAEGLGVLAVVVGLLCIFLR"
     gene            28713..29270
                     /gene="atpF"
                     /locus_tag="VIF08_04950"
     CDS             28713..29270
                     /gene="atpF"
                     /locus_tag="VIF08_04950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY8135369.1"
                     /translation="MPVFAGAGGVLDPILRLFAEEAEVGFQLNLFWIIAQAGSFLLFF
                     VILYFAAFKRIGGVLEERRSKIEQGLRDADDARKEREQAASERLAVLAQARNEAEDIL
                     SRAQKVADESRERDMAETRAQLEQLRTQAAADIASEKERALADVRSQVAELALAAASR
                     VVGETMTGEREKRLVNEFLTEVGKK"
     gene            29273..29830
                     /gene="atpH"
                     /locus_tag="VIF08_04955"
     CDS             29273..29830
                     /gene="atpH"
                     /locus_tag="VIF08_04955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY8135370.1"
                     /translation="MAGTRRSSTARRYAEAVFEIAERDDTVERWLTQLNDVAEAVSRK
                     EVVRRLEDPHVPLEARAKALHDVAGAQMLPQMSNLLGLVLRRRRLDTLPQIAREFKRL
                     YNRRAGVVEATATSALKLDAQEVADLTARLENMTGKKIELSTAVDANILGGIQVRIGD
                     TLYDGSVRGRLERLRARLESGALTA"
     gene            29884..31416
                     /gene="atpA"
                     /locus_tag="VIF08_04960"
     CDS             29884..31416
                     /gene="atpA"
                     /locus_tag="VIF08_04960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY8135371.1"
                     /translation="MAIRSDEITTIIKSAIDQFDAGVETRSVGTVVEVGDGIAQVYGL
                     EGALASELLEFPGGVMGMALNLEEETVGAVILGDFKQIKEGDQVKTTGRVVEVPVGAA
                     LIGRVVDPLGRPLDDKGPINAAATRPVERIAPGVIVRKSVDTPVQTGVKAIDALIPIG
                     RGQRELIIGDRQTGKTAIALDTIINQKGKGLVCIYVAIGQKLSTVAKTVAVLEQYGAM
                     EHTIVVVAGAEDPAPVQYIAPYAGAAMGEEIMENGVELNGTLVKDALCVYDDLTKHAW
                     AYREMSLLLRRPPGREAYPGDVFYLHSRLLERAARLNEENGGGSLTALPMIETQANDV
                     SAYIPTNVISITDGQIYLESDLFNAGQRPALNVGISVSRVGSAAQTKAMKKVAGPLKL
                     DLAQYRELAAFAQFASDLDKATRDQLTRGEKTSEVLKQPQYEPLAVEKQVAILWVVTN
                     GYLDDVPTPRIRDFEQRFYRFLETEHPAVLDTLGKKPELTDDVVSGLKSATEAFKQTF
                     VS"
     gene            31440..32294
                     /gene="atpG"
                     /locus_tag="VIF08_04965"
     CDS             31440..32294
                     /gene="atpG"
                     /locus_tag="VIF08_04965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY8135372.1"
                     /translation="MPSQREIRRRISSVRNIKQITRAMQFVAASKLKRAQDATLQSRP
                     YADKIDEVLADLAAVLGADDHPLLARREGGTRAIVLLTTDRGLAGSLNTNTIRYTAEL
                     ITKETDEMRMVTVGRKGHASMRRARVPIAATFEGYGDRPTFSSVVPLARLLTDDYLAG
                     TYSEIDLVYPRFVSTLVQRPHLDRLLPVEPASDADEGIPGNQFIFEPNADAVLRQLLP
                     RYVATRVYQAVLELTASEQSARMVAMKNATENAQELIEDLTLTYNKVRQSNITREMIE
                     IATGASAS"
     gene            32317..33738
                     /gene="atpD"
                     /locus_tag="VIF08_04970"
     CDS             32317..33738
                     /gene="atpD"
                     /locus_tag="VIF08_04970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY8135373.1"
                     /translation="MAVAAPSKKGVRTGRVIAITGPVVDIEFPQDHLPGIFNAVEIMR
                     EGLEPLVVEVQQHLGNNWVRTVAMTTTDGLARGAEAVDTGGPISVPVGPATLGRVFDV
                     LGRPIDNKGPVDAQQRYPIHRAAPSFEDQTTATEVFETGLKAIDLIAPFAKGGKASVF
                     GGAGVGKTVVIQELIRNVAEEHGGYSVFAGVGERSREGNDLLHEMTESGVIAKTVMVF
                     GQMNEPPGARLRVGLSGVTFAEYFRDEGRDVLLFIDNIFRFTQAGSEVSALLGRMPSA
                     VGYQPNLATEMAGLQERITSTKKGSITSLQAIFVPADDYTDPAPATTFAHLDSSIRLE
                     RYLTELGIYPSVDPLTSTSRVLDPNIVGQEHYETARETQRVLQSYRDLQDIIAILGVD
                     ELSEEQKITVARARRLQRFMSQPMFVAEVFTGRPGRYVAIKDTVASFKEILEGKADDL
                     PEQAFFLAGTLDDVRENAKKLNA"
     gene            33739..34167
                     /locus_tag="VIF08_04975"
     CDS             33739..34167
                     /locus_tag="VIF08_04975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY8135374.1"
                     /translation="MPLLLEIVTPERQVFSEEVDSVVCPGIEGELGVLPHHAPLLTTL
                     GVGELRIRRGGSEEYFAIAGGFLQVRPDKVVVMAETADLASDIDLEKATAARREAEQA
                     LAEGFEEPADLARARASLQRALLRIRVAERHRREGPRSRG"
     gene            34191..35531
                     /gene="murA"
                     /locus_tag="VIF08_04980"
     CDS             34191..35531
                     /gene="murA"
                     /locus_tag="VIF08_04980"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8135375.1"
                     /translation="MADRLFHWEYQELPVERQAFRVTGGRPLRGKVQISGSKNAALKM
                     LAAATLTGERCRFTNVPEIEDVRVMADVLRDLGVVVDHPAPNTYEVAAGDVEWLFVPL
                     EAAAKMRASFILLGPLLARFGRVIMSNPGGDRIGRRPVNLHVDAMRALGADIEYRNGY
                     YFARSNGRLRGSEIAFPQVTVMGTENAILGAALAEGRTTIRPAAQEPEIDDLILFLQK
                     MGADIERTAPDTIVVNGQRRLRGADHHVLPDRIETGTFLIAAAVTGGEVTLEGAPCDH
                     LGAFLDAMTDIGVPVACGRNTIEIACSEDAVRSFKARDIETSPYPGFATDLQSPTSVL
                     LSQATGTSRLHETIFEDRLEWLAELRRMGAQVDLLDAHNASIKGPVKLHAADVEMGDL
                     RAGASLILAALTAEGTSTIHGAHHVRRGYENIEGKFLDLGAQIEHVSEEAREGD"
     gene            35548..36537
                     /locus_tag="VIF08_04985"
     CDS             35548..36537
                     /locus_tag="VIF08_04985"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8135376.1"
                     /translation="MKIGIDLGTANILVYVKGRGIVAREPSVVAVSDDNHIVAVGEEA
                     REMIGRTPGNISAIRPMKDGVIADYVITEAMLRYFIRKAGRALTKPDVMISVPSGVTS
                     VEKRAVRDAALKAGAREAYLIEEPLAAAIGANVPISGPSGNLVIDIGGGTSEIAVISL
                     GGIVVARSVRVGGNRFDEAIANYVRKKYNVMIGDRTAEEVKMQIGTAMPLERELHMEV
                     RGRDLIAGLPRTVPLTSSEVMEALEQPLQQIIGAVRAVLEETPPELSSDIIDKGMVMT
                     GGGSLLRNIDKLLTQVTGVPCHVADNALNCVALGTGLALEHFDFFRKSLVQRI"
     gene            36573..37289
                     /locus_tag="VIF08_04990"
     CDS             36573..37289
                     /locus_tag="VIF08_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEY8135377.1"
                     /translation="MEPDSTRPVRSLVDLHCHTSASFDSLSKPADVARVAAERGLTHL
                     AITDHDRIDGALAARDAAPAGLAIIVGQEVRTTTGDLIALFVERPIPPGLAPADAAQR
                     IREQGGAVGLAHPYDRFRAGAARPGWEDELERLLPRLDYVETWNSRLMLGDGNRRAAE
                     LAHAHGLPGAAVSDAHTLLEVGVSYTVVDGPLDSAADLRAGLVGARIVTSHGSRLVRL
                     ATPLAKVIQRMRGNGRVPVA"
     gene            37286..38410
                     /locus_tag="VIF08_04995"
     CDS             37286..38410
                     /locus_tag="VIF08_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY8135378.1"
                     /translation="MSDPAADQPSAEARVVDEPIVDYPGVHEESTPPQPLSRRLRDPR
                     TIVSLVLPIVLLVLVALSLKDLDLNELTQTIVRANPWFLIAAFAIYYAGFPLRGYRWQ
                     LLLRRAGTHISLRDSTEIMFISWLVNCLVPAKLGDVYRAWLLKANFTVSLSRTFGTVF
                     IERIFDLFAIAILGLGAAFWSFRGNLPTAVQVVIAIGLAVVLLLSVGLYTVRNFGKRA
                     LLRVPLPRKKQLLELYEKFEEGVFAINPRQVPWLAVVTAAIWATEAIRLLLVIEALNF
                     RDVHLGISGAFFVALSASLLTAIPLTPAGLGIVELGIVGILTVIYGVDQTNAVAITLV
                     DRAISVFSIWFTGGLIYALSRKTKANVTRRPADRASAAGG"
     gene            complement(38499..38930)
                     /locus_tag="VIF08_05000"
     CDS             complement(38499..38930)
                     /locus_tag="VIF08_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8135379.1"
                     /translation="MSVQNGRAFNDWLRTQLKAKKMSQRQLAQQSGVDHSTISRLVRG
                     DRTPSLGTATKLARGLRELRDETETPQYFGLVASTASHPTARVEYALRSDDLLSEPQV
                     RQVMEYYLALRMRRLAAARQNGAVTTDPRQVLAAARMPNRN"
     gene            complement(39035..40000)
                     /locus_tag="VIF08_05005"
     CDS             complement(39035..40000)
                     /locus_tag="VIF08_05005"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY8135380.1"
                     /translation="MTDFKRLIGPHLQLGQGLLKAADRAQAIGANAVQVFTDNPVAWR
                     RRDELPAELPEFRQRLAAAGIEQLAIHAPYLVNLCGANEEFWARSVATMAHELVVGRE
                     YGAQNVVMHIGSHRGDERDSAIERLVRGLAAVLREADQSEPAPGLLPRLVLENAAGTG
                     DGIGSSLEDLADILAAADAAGLPMERLGVCLDTAHLWGGGYDVGSSTWIEWLTERAER
                     LLGRANVVMLHLNDSRVELGSRLDRHEHVGAGKIGRDGLRDLLNHPWLGALPTFLETP
                     GMDAGYDAINLERALMLADGQELPVLPPEAFNLRRGAARSAPPGA"
     gene            40054..40659
                     /locus_tag="VIF08_05010"
     CDS             40054..40659
                     /locus_tag="VIF08_05010"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8135381.1"
                     /translation="MDPDLLTALKALSDASRLRIVGLLADGRRMAVEQLAAALKVTPA
                     TVVHHLRKLREAGLVESHPRPPYMDYSLRLGRLSEIGASLHRIAREQTGAAEVDPTLP
                     DWATPADAKVLRAFFDGERLTSIPVQHSKRLVVLRRLAETIFEKKRKYPEKEVNQLLA
                     VRHPDAASLRRYLVDEGFMARKQGVYRLQPRKDWPKVGSGR"
     gene            complement(40684..41337)
                     /locus_tag="VIF08_05015"
     CDS             complement(40684..41337)
                     /locus_tag="VIF08_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135382.1"
                     /translation="MRRLLDVLLPPACPGCGREGAAVCSKCLPSLARRRDEPPGVPLG
                     LATTQPRGLVQLEWCCSYSGVARAALAALKYDGELRLVDVLAEQMAARWGRAGIGGDV
                     LVPVPVHAARLKERGFDQAELLARAAGRLLKMPVATVLERAARTAAQHSLGRRARAAN
                     VGGAFSIKAGGRRQVSGGWIVLVDDVVTTGATLSGCAAALYAAGAHAVSGLALARER"
     gene            41446..42618
                     /locus_tag="VIF08_05020"
     CDS             41446..42618
                     /locus_tag="VIF08_05020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY8135383.1"
                     /translation="MTNRRWAGPLALGLAALGVLVLVGVLAAWFNESRGWAYDFHPYY
                     DGALRLLANGSPYQAVTLDGPFSPGPKGLYLYSPVLAALFVPLTWLGEGSAIVVWATL
                     HIAALVALCALMPISRNLRLATFGIACLSAPVLYDIDLGNVSLFVTLAAVLVWRWLDR
                     PGSGIVLALALLVRPAMAVICGWWLLRGLWRPVAWAVGTFAAVAAVSLVWLRPDVWWE
                     WLTVLRNVSNVTGVKANVDLGSAVLMVGGSAEVAQIALYGGYVIAVAAILLSLRRDRE
                     LSFVVTLMATLLLSPLLWDHYLTNLLIPAAFLAARGRAWGLALPLLCWAPQIIAVLIP
                     EMRTHADGALALVAVLGVVLPFLAPDSDARAGFFWERRWGPLRGRRQGDVIADAEA"
     gene            complement(42571..42963)
                     /locus_tag="VIF08_05025"
     CDS             complement(42571..42963)
                     /locus_tag="VIF08_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY8135384.1"
                     /translation="MSDKKPSNRVELHAAGSAAPLWQLDVADSWLARLRGLIGRRGLA
                     AGEGLYLPGTNGVHMFFMRFAIDVVFVGAPRADGARMVVALKPNLRPWTGLVPWVRGA
                     RGAVEVPAGSLTSSGLSVGDYVTLAPAA"
     gene            complement(42988..43911)
                     /locus_tag="VIF08_05030"
     CDS             complement(42988..43911)
                     /locus_tag="VIF08_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8135385.1"
                     /translation="MLPIIMAGVAALGVLLVFLALAGSKQMDPVQARLSQLGSMQART
                     LEEIELQQPLFERTLRPLATRLSGIAQRFASPSKVSRTERRLSMAGNPGALRTIDFLG
                     MKLVMAGLFGAGAFAVFGILMGNAPFGFIGALAIGGFGFLGPEIWLGRRIKARQKAIL
                     LATPDTLDLLTISVRAGLSFDGALAKVVEKTHGPLADEFRRALAEIRVGKTRRDALRD
                     IVGRTEVQALSNFIGAIVQAEQLGVSISKVLQVQSEQLRIERRQRAEELAAKAPIKML
                     FPLVGCIFPSMFIVILGPAIILIALNMSNGQ"
     gene            complement(43914..44915)
                     /locus_tag="VIF08_05035"
     CDS             complement(43914..44915)
                     /locus_tag="VIF08_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8135386.1"
                     /translation="MDPLVILVAAGAAVGVLLIFVSLAGGSDVNARLERYAAGDSDRH
                     DTEAKRDLGERIATSAALANFNRIVERRDWGSNLARELARADLALKPTEFLAIRAGAI
                     LGLPLLFVGLSPLIPLFASPIAWIAALLVGYWLPKFWLGRRKGKRLKAFNTGLADTIM
                     LLANSLRAGSSFLQAVEMIVRETRPPMSTEFARVIREVNLGLPLDEALANLQRRVRSD
                     DLDLMTTAIAIHHTVGGNLAEILDSIAFTIRERVRIKGEIRTLTAQQRMSGYVVGFLP
                     IALVLLLSVIAPTFMAPMLQKPPELLGLPAGMFILAGGGFMMLIGFILIRRIVDIEV"
     gene            complement(44918..46396)
                     /locus_tag="VIF08_05040"
     CDS             complement(44918..46396)
                     /locus_tag="VIF08_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY8135387.1"
                     /translation="MSLLRRIESARPAADMPGIAPADPAASQNPGSQPPVRPPAGLER
                     LPTAPPPAPGSPGQGGAPTTGRMLAQVPLRESFRDAKFRIQQRVISDIDPKLDLANAV
                     EVRREIEEAFGRALDAEGLALTRAERTRMLEQITDEIVGLGPLEPLLRDEGITEVMVN
                     GPRQVYIERSGKLELTNVTFQNDEHVMRIIDRIISPIGRRVDESSPMVDARLVDGSRV
                     NAVIPPISLVGPVLTIRKFAATPLTTDDLIRFGTSTPEMFDFLRACVEARLNVFVSGG
                     TGSGKTTMLNVLSSFIPNDERIVTVEDAAELQLRQEHVVTLESRPANIEGKGAIPIRE
                     LVRNALRMRPDRIIVGECRGGEALDMLQAMNTGHDGSMSTGHANTPRDMLARLETMVL
                     FAGVELPLRAVREQIASAVDLIVHQDRLKDGSRKITNITEVQGMEGDVIVLQDVFMFE
                     QTGIVEGKVQGRLRPTGVRPRFSEKFEAHGIHLPPRMFGQPI"
     gene            complement(46411..47679)
                     /locus_tag="VIF08_05045"
     CDS             complement(46411..47679)
                     /locus_tag="VIF08_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8135388.1"
                     /translation="MSTTEPIRVVIVDDIIETRDHLAKLLSFESDMAVVGSVGSGQEA
                     LSLATQAPVDVLLLDINMPGMDGIATAEQLSLRVPTASIIMMSVQGEPDYLRRAMLAG
                     AREFLVKPFSSDELAASIRNVHQRERQKLDRVASVRPAASGNGHAEPIQAHNGQVVTF
                     FSPKGGVGRTTLAVNFAVAAKAELGKKVALVDGGLQFGDVGVLLNLNPKNQTIADVAR
                     EMAGGDMETLETTLVDHSTGVRVLMAPPSPEMAELVTPDHVSKIISALRLTHDLVVVD
                     CSPLLQDVTLAFLDQSDIVLTVLTLEITNIKNIRQFLALVEQLGYPEGKVQLLLNRAD
                     SGYGIRLHDVESSIGRKISHSIVSDGRTVVYALNRGVPFVVGARQTRVAEDVVRLTKT
                     IVGGEQVQPGQAPARAAQRKSGGLFQWRAH"
     gene            complement(47707..48561)
                     /gene="cpaB"
                     /locus_tag="VIF08_05050"
     CDS             complement(47707..48561)
                     /gene="cpaB"
                     /locus_tag="VIF08_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY8135389.1"
                     /translation="MKRANRFMLIAGVLLAAVAFVAVLAFGGLGSPTVDAPPADVNVV
                     VAAVDLTLGTELTADKLTTQPRPQADASGTYQHPEELVGQIVRRAVPAGQALTASDFD
                     TTAVAQIAGSIQPGLVAIAIPLSKVDAVGALLQPGDRVDVLISMRDTDSLNPQVLPNP
                     NYGHVSPDGTVVDTNPYISVDEFMNNTTVKVVVQNVQVLAVMARSNDPNNGVTPNGVP
                     EPDVVVVLAVSPQQSEVVRFAQLDGNVSLLMRAPGDSGAPDTTTSGITLKELVDQWGV
                     LPPKSVTP"
     gene            complement(48823..48957)
                     /locus_tag="VIF08_05055"
     CDS             complement(48823..48957)
                     /locus_tag="VIF08_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135390.1"
                     /translation="MKQAGQGLAEYALILALIAVASIIAITFMGGSINSMLSDLGNRM
                     "
     gene            complement(49170..49310)
                     /locus_tag="VIF08_05060"
     CDS             complement(49170..49310)
                     /locus_tag="VIF08_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135391.1"
                     /translation="MDDRGQSLAEYALIVALIVIVALVALTLFGTQINDILSIIADTI
                     NG"
     gene            complement(49421..49585)
                     /locus_tag="VIF08_05065"
     CDS             complement(49421..49585)
                     /locus_tag="VIF08_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY8135392.1"
                     /translation="MLQYLVSLIRSDERGQGLAEYALILALIAVLAIAAVTFLGGQIN
                     SILSNVGNKI"
     gene            complement(49781..50881)
                     /locus_tag="VIF08_05070"
     CDS             complement(49781..50881)
                     /locus_tag="VIF08_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8135393.1"
                     /translation="MDRTLPQLAGELAADQATLERELAEIEMLLRQTATESERHEARR
                     IQAEERVTALERNEPVAPELAEARTALLAQTRRVTLMQAQLDVLGGKQRALQRFRDRL
                     AEILPVMRAVPPDGIVAASAPNTNPGEDLAAQEQLRREIARQMHDGPAQSIANIALQA
                     HVVQRLFERQPEQAQAELAELVRMVEHALAATKDFIFDVRPMVLDDLGLVPTLRRSSL
                     ERGRRAGLAVRFESVGADRRLRSDLESALFRIIDDAMSGYLSGAPAALSVKLNWTAGS
                     VSATIQASSAQATQSAATRAHAVVAAARHERTLPAALASMIREQERDDTLRATGLPEG
                     AWSDIQLRADAAGLQVTLSSDRSTLEVSTATG"
     gene            complement(50915..51661)
                     /locus_tag="VIF08_05075"
     CDS             complement(50915..51661)
                     /locus_tag="VIF08_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8135394.1"
                     /translation="MNEADYSGPDRRRGGPDRRNPEQRTKILLVDDHALFRMGMRQML
                     DKEPDFDIVAEAEDAREAFSMAVEMTPDIVLLDLSLPAPGGIETTQRIKREVPSAGII
                     ALAVAEDEDQLFEAIKAGAAAFILKDINPDDLVTIIRRVRAGEYLINDKVFSRPSVAS
                     RVLKEFRELAVYGQEAAPIFAPLSPREVEILDNIAKGMTNKQVAYALTISEQTVKNHM
                     SSILRKLSVNDRTQAVVYAMKQGWIRIPED"
     gene            complement(51658..52521)
                     /locus_tag="VIF08_05080"
     CDS             complement(51658..52521)
                     /locus_tag="VIF08_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8135395.1"
                     /translation="MSDNERDVVRARTTSRDVPATYTPNSMENGTLAANRQVQADASP
                     VPEAESALAADATPLEADPRIAMALLGAQEQERSRLAEELHDGPAQAFANAIFQTQLV
                     ERALREEPAAAVTELTALRTLLERELDTLRGYINQLRPSLGEAVGLDDALRDSASAIS
                     QRSGLPIELRLEAAGNYLHAAARTVVLRVAQEALRNIAKHASASRAWIHTSEGVAPDG
                     SPTWVLEVGDDGRGFDYTTVTANPNRRHFGLRFMRERAELLGSSLAIKSHHAAGTVVR
                     LTIALQERSHQ"
     gene            complement(52518..54146)
                     /locus_tag="VIF08_05085"
     CDS             complement(52518..54146)
                     /locus_tag="VIF08_05085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8135396.1"
                     /translation="MTATAPTMGAVHAHVWRRPLVASATTFAALYVLGLAIGLLVIAL
                     VRDPLSEGTSYYVDVARNLVNGRGPVIDAIWSYATPPLTLPRPAFELWQPMASFVAAA
                     GLALFGPSFSAAQMGFVLLGATLAPMAWLVARDTARALKLDERRSMTVELGAALLTAS
                     SAPLLLATGAPDSTIPFAVLGVAAGLLIPRAVAGERKNVIALGVTLGLAYLTRMEAVW
                     LGVAFVVGSLLAGQSVARTFATSTGVAAIAALVALPWWARNLAVFGTPLPGQVTDNIF
                     LTRNEQIFAYTDQPTLSGFLAQGLLTILGNIGGALWHDAVDVLVVGAGPVAVIGLVTV
                     AAALAGRTSLSRQGPLAMLLLSGAITFFATSVLFPVATLWGTFEHAAGPLHVGLIVAA
                     VVGLDALVARIRVWRSWPRSNSWLAPAALALATIPITSLALVGAANAALTDSAHIQSV
                     AAALPGGAEPVITDRPIWLSSATGRPALALPDEAVGSITQLARDFNAGLVVVTEPRGA
                     FPGVLRSAEAAACFVEVPGMPSESAAFTVNEACR"
     gene            complement(54143..55783)
                     /locus_tag="VIF08_05090"
     CDS             complement(54143..55783)
                     /locus_tag="VIF08_05090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8135397.1"
                     /translation="MIALGLFAAAFLVRVAVGAAFAGPAYPDSYYYYSVAQSLAGGHG
                     LTIDYIWNFVDVGGQIPTDPTLPIPSNGHWLPLAVLVQVPFIWVLGTTALASQLPMWL
                     IGAAAAPLTYFIARDAGLETRLSVAAGLLAAVPGGLTPFFGQPDNFGLFMTLGALALW
                     LCARGMRGDRRAFVLGGAVVGLAALARNDGVLLGVPFALAFARELVRGRGRGQVIGWS
                     AAIGCIGLFGLFYGPWLYRQLEVFGSIAPSAANGRILWISDYGQLFSVTAPATPATLL
                     ADGWGPFLASRLSGLLSALGQFALLPLVVVLTPFALIGASARRRDTNVVPMLIYGLVL
                     FAASGLVFAVHVPYGTFIHSAVALLPHTFVLVVIGVGGAVRWIAERRPTWDAATATRR
                     FAYGAVAVAFLGAVGQSLITIGEWRAVRDVESRLAAALIVAPHSDRVMSGDSGAYHYL
                     SGHPGVISPNDDLATIESAMRAYDVRWLVLEQGSIVPALVPVLNGTVHPTWLSAPVAV
                     VPSRVSAPVATINPVTVASPAGAVYAVCLTPEDPRCEQ"
     gene            55908..56777
                     /locus_tag="VIF08_05095"
     CDS             55908..56777
                     /locus_tag="VIF08_05095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY8135398.1"
                     /translation="MTALFRQPAQRRILVHAAVVIGLVFAAYIFLVSAAQKGSLGFDI
                     VAYWSVDLAHPYHGNVGDLGFFAYAPPIALLLAPFTALPFPIFVTGWYVLLLTTLAWL
                     GRRQMLILLAFPPVALDLYHGNIHLLLAAAIVLGMRYPAAWSFVLLTKVTPGIGLLWF
                     AARREWRSLSVALAATALIVAGTLVFLPAQWLGWLQMLTDSAGTAPPWPALPVPLVLR
                     LPLAALIVLWGARRDARWTVPIAAAISLPALWPGGFAILAACWPLRSAHSVQARPKAA
                     PQAGSMDVQPAAA"
     gene            56755..>57482
                     /locus_tag="VIF08_05100"
     CDS             56755..>57482
                     /locus_tag="VIF08_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEY8135399.1"
                     /translation="MSSQPRPSVTLVLPAYNEEARIGSALNELFGYLRRGGPPREGSR
                     PATELGLTDILVVDDGSTDGTAQIVATRPEFAPGSTPQLRLLKRSHEGKGAAVRAGML
                     EATGDFVCFADADFATPPDQLPLLTDALTTHDVALGSRVQPDGTDRRQSQPFHRRLIG
                     RFFHWLAAVWVTGPVPDTQCGFKGFRREVAHDLFGQQRLTSIVFDAELIHLARRRGYS
                     IANVPVQWSDKRGSRMRVRPGLALR"
BASE COUNT         9037 a        19516 c        19524 g         9405 t
ORIGIN      
        1 gtgtcgaaga ggttgtcgat cttgagcagc cgctcaatct tggagatgcc ctcttccgtg
       61 ggggcgacgg ccttctcctt gagatcgacg aagaagtcgc cgccctcctc cgctccttcg
      121 ggccgcttct ggagtcgcgg cacaagacgt gcgaactggt agtagaggtc ttccgattcg
      181 gcggcctggc cgctgatgat gagcggcgtt cgcgcctcgt cgatgaggat gttgtcgacc
      241 tcatccacga tgccgaagta gcggccgcgc tgaacacgct ggttgagctc ggtgaccatg
      301 ttgtcgcgca ggtagtcgaa gccgaactca ttgttcgtgc cgtaggtgac gtcggccgcg
      361 tacgcctcgc gccgctccac cggacgcagg ttgagcaacc gctcatccgt gccgcggtgc
      421 tcggggtcgt acaggtacgc ggtctcgtgc tggatgaccc cgaccgtcaa gcccatgccg
      481 tcgaagatgg ggcccatcca ctgggggtcg cgcttggcca gatagtcgtt gaccgtgacg
      541 acatggacgc cgcggcccga catggcgttg agtgctgcgg ccagcggcgc gacgagggtc
      601 ttgccctcgc ccgtcttcat ctcggcgatc ttgccctggt gcaggaccac gccgccgatc
      661 agctggacgt cgaaatggcg catgcccagc ttgcgccggc tgatctcgcg tgccgcagcg
      721 aagacctcag gcagcgcgtc gtcgaggacg ccctggagat gcttgacgtc ggccgtctca
      781 cgcgctcgcc ggagctcgcg ccggcgctcc gatgaatcgg cattgagctc ttcgtccgac
      841 ggctcgcttg gtgcgccgtc ctcgtggatc tgctcgcgca gctcgatcat ccgcgcgcgg
      901 atctccgcgt cgctcagcgc ctggaactcg gcttccagtg aattgatctc gtcgacaact
      961 ggctggagct tgcgcacctc gcgcccgttc gcgtccagaa accgggaaag aaggttcggc
     1021 atgagaccgc caagtatagg ttgggcccct ccgtccctcc gctgacccgc tatttggccg
     1081 cgctcgcgaa ctggttcagc ccgagcagga aagtgcggta ttcgactgtg gaagcggaaa
     1141 aaccggaatc gaccagcagg ctgcggatct cagcctcgtt gaagtagtgc ctgtggtcgc
     1201 ggatctcctc tgcgtcgatg atgtgcaggc ggtaggccat gaactccagc aacggccgcg
     1261 acgcgcgcga tggtgaggtg agcagcagca gcccgggcga gcgcagcacg cggtacgcct
     1321 cacgcagatg gagccctgga tcgtcgaggt gttccagcac ggccagcgac atgaccacgc
     1381 tgactgaggc gctggcgatc tcgagcggtc ggcccaggtc cgtctcgatc agcgtgatgc
     1441 ccggcggcgg gggctgcccg agcgccaggt caacagcgat gcccgctccg atccgtcgac
     1501 ttcggtgcag gtgccgcagc agccggtagt catggccgca gcccaggtcc gcggccacgg
     1561 ccgcgtacgg cgggaattgg cgcaggatgc gccgcgcacg gaggcgaaag atcaggtcgt
     1621 caagtgcgga gccgccatcc tggcggaatg acctttgccg tcgcgatccg ctgtccaccg
     1681 cgccgatggg tcagcctccc tggccgccga cttggtcgac tggttcacgg agtgcatcaa
     1741 tttgctcgtg ggctatgagg tcgagcttgc tctcgatctc ctggaagcga cgactgatgg
     1801 aactcagcac catgcccacg acaccgagca ggatgccgac gacctcgagg accgccgcca
     1861 ggacggccga cggcagtcgc agcaccaggc cggttcgggc gaactcgagc acgacctcga
     1921 tgcccggaat gaggccgatc gccatgaaca ccaggccaat gccaccaaag aatgtcagcg
     1981 gcttgtagtc gcggaaaagc aacaggatgg tccaagcgat gcgcgctccg tcgcggaaca
     2041 ccttgatctt gctggcgctg cccttgggcc gcgaacgcag gtcgatcggc agctcgacga
     2101 tgcgcatgct gcgttcaatg gtcttgaccg tcagctcggc ttcgatttcg aagcccgacg
     2161 ccgcgatcgg cacggtgcgc accagctgac gcgacatgac gcgcagcccg gacaggatgt
     2221 cggtcagccg gacccgcagc aggaaacgca ccagggccgc gtacatcctg ttgccgaacc
     2281 gattcaggaa gtggaactgg ctgttggtgc cttccatcgt gcgcgagccg acggtcatgt
     2341 cggcccgccc ggtactgatc ggtgcgagca gggcgtgcac cgcctcggcc gggtaggtgt
     2401 cgtcaccgtc gaccatcacg tacgcctcgg cgtcgatcga gcggaacgcc gtgcgcaccg
     2461 caaaaccctt gcctcgacgc gtttcggtca gcacctgcgc accgtgctcg agcgcgacct
     2521 tggccgtggc gtcggtcgag tcgttatcga tcacccagat ccgggactcg ggcagctcac
     2581 gccggaagtc gtcgatgacc tggccgatgg tctgcgcttc gttgaggcag ggaatgatga
     2641 ccgcgatgcc tctgccggtg gaacggatgg ccggcggccg cgtctgaacc agggtcacgc
     2701 cctcaccgcg atcgccaggc agccggtctc agggaaatgc atggggtatc gataggctga
     2761 gcgcagaaaa agtgcaatgg tcccggaagt ataggtacgc gatttgtctg gttatgagtg
     2821 gcgccagtga tctgccacac tcggcatatt ccatgagcag cacgtccgtc accatggctg
     2881 cggagacccg atcgacgctc gtcgtccgca acgagcgcgc ccgcgttttc ggtgcgtcat
     2941 tggcggtctt catcttcact ttcctcgttt actacctgac cggtccggtc aacacgccgt
     3001 acgacttcca gctctcccag gccaacaaca tcatccacgg ccatctcgac atgacggccg
     3061 agtacacgcg caacctgaat gtcctcgagc gggtcctgta cgacggccaa ggcttctgcc
     3121 tgccgatcaa tgacccgcgc gggccggaga ttgacgccct catcccaaac gcccgcttca
     3181 gcgccgattg caagcactac atgcagcact cacttgggcc ggcattcctg ctgctaccgc
     3241 tcgtgctgat cttcggcatg accgtcagcc aggcgctcgt ctcggctttg atcggcgcgg
     3301 cgggcgcggt tgtcgtattt gccatcacgc gccacttcac gagctcactg cggacgcaac
     3361 tggcgctgac cgtcctgggg gtcctgggca cgcagatctg gttcagctcc gccgacggca
     3421 gcgtgtggca tttcgcccat gcaactgccg tgttcttcgt cttcagcggc atctacttca
     3481 cggtcgtgcg gcgcaacccg ctgctcgccg gggccatgtt cggcgcggcc ttcatgtgcc
     3541 ggccctcgac catcatcggc ggcttgttcg cgctggtggc gttctcggac atgtggctgc
     3601 ccgccgcgac cgaggccggt cgctccatcc tgcgccgtat ccagctgcgg cccctggtcg
     3661 cgctggcgat tggtgtcgcg ccgttcctcg tgctcaattc gctggtcaac tacctgcgct
     3721 tcggcagccc attcgagtcg ggctacagct acaccgagca gctctaccag gacaacctga
     3781 cctgggtgta cccgcacggg ctcttcagcc cgcagtactg gtcgcgccac gtggcaatcg
     3841 tcttcgagca gatgcccaac ttctcgggca acccgccgtt catctggcct tcgtgggcag
     3901 gccaggccat gtgggccacc tcgcctgcgc tgctgctcgg cctgttcgtg cacctgcgca
     3961 agtgggtctg gccggcgcgc ctggctgcca tcgccatcgc gctctcgggt gcggtgctga
     4021 tcagcggtgc agccggcgcg cagctgggcg ttgtccggtg gacgccggcc agcgtgccat
     4081 tcgcgctgca cctgtggcct ttctggatcg tgatcggtct cgccatcgtc ctgtcaatca
     4141 cgaaccgcga ccggctcgtg gtcgcgtgct gggcggcaat cgtgcccatc gtgctcttca
     4201 tctggatgtt cgccgcaacg ggttgggccc agttcggcta ccgctacggg ctggacttcg
     4261 tgccgttcct gttcctgctc gccgtgatca tggtcacgcg cgggatgcgc ctgacgcgct
     4321 accaggtcgt gctgatcggc gcatcggtgc tcatcaacct gtggggcgtg ctgtggatcc
     4381 tgaagttcgc gatcgcccca ggtggcccgc tcaacggctg gacctgggtc agctactgac
     4441 ttcgagcgga aagccccagc gccgggtgga gatcgcgccc ccggtctagc tgatcgagcc
     4501 ccagaagccg gtgccgtagc cgaccacgag caccacgaag gtcaacaccg cgaggaccac
     4561 ccagaaggcg acggccatgc gcgtgctcgt cggggcctgc tcggggttcg ccagctcggc
     4621 gggcttccag cgccgcgagc gcaggcggct gcgccaggtg cgctgctcga ccgggctcgg
     4681 ctcgagaccg ttgggcgtgg ggcggtagag cttcagactc atgcgcttgg tcctgggcta
     4741 cccggcgtgg gccgcggcgg gcgcgggttc accgtggccg actccgtccc gaaaggtgtc
     4801 atcgtcctcg ccgcgcggcg tctcctcgtc ccacaggatc atctcctgca cgagcgacat
     4861 gtcgctgctg aatagagcgc cgaccgactc gccgcggtgg atgcgcctta tcgcctcgcc
     4921 gataagcggc gcgacgctca ggaccttgat cagcggcagc tgcttggccg ccgccagggg
     4981 caccgtgtcg gtgatgacga cctcggtgat ctcggaggcg cgcagccggt cggtggccgg
     5041 gtcggacagc acgccgtgcg tggcacaggc gtagatatcg gtcacgcctt cgcgaatcag
     5101 cgcgcgctcg atctccatca gcgtgctggc ggtgtcgatc tcgtcgtcga cgatgatcgc
     5161 gcgcttgccc ttgacctcgc cgatgacgtt gagcagctcg gctgtgccgc ggttgtcggg
     5221 gcggcgcttc tcgatgacgg cgagcggtgc gtcgaggatc tccgcgaagg tccgcgcgcg
     5281 cttggcgaag ccgaggtcgg tgaccacgac cgggtcttcc aggttcagct tcttgaagtg
     5341 gctggacagc atgtggaccg cggtcagctc atcgacaggg atcgagaaga agccctggat
     5401 ctggccctgg tggaggtcca tggtcagcac ccggtcggcg ccggcaacgc tgatcatgtc
     5461 ggcgacgagg cgcgccgtga tcggcacgcg cggctggtcc ttcttgtccg agcggccata
     5521 ggcgtaatac gggatgaccg ccgtgatccg cccggcgctg gcgcgcttga aggcgtcgat
     5581 catgatcaaa agctccatga tcgacttctg gaccgggctg ttggttgact ggacgatgaa
     5641 cacgtcgcgg tcgcgcgcgt tgtcctcgat cttcacgaag atgttctcgt tggggaactc
     5701 gaagacctgc gcgtggccca gctctcgacc caggtagcgc gcgatcttgc gcgccagctc
     5761 gggattggag ttgcctgtgt agaggtcgta ttggtcgctg tagacggtcg cggcacggcc
     5821 gccgaatgcg ctcacgaaga ccctccagcc gggccgtcat tgtcgggcat gggttcgccg
     5881 tccgccagcc ccggttccac ggtatcgcgg gtttcagcgc ggaagaccgc aatgccggcg
     5941 acgatgtcgc cctcgttcag gcgcatcacg atgacgccgc gcgctccgct gccatagcgg
     6001 ttgacgctct tgacgtcagt ccgcacgacc tggccctcgc ggctgatgag gagcagctcc
     6061 tcatcctgct cgtcgaccaa ctggacggcc gcgaccttgc cggtcttgga gccctcgatc
     6121 gggatgagca tcacgccctg gccgccgcgg tgcttgcgcc ggaactcgga cacctcgacg
     6181 cgcttgccgt aacccgtctc tgtcagaacc agcacgtcgt tgccgggctt gatcacgccc
     6241 atgttcacga cctggtcgcc ggcgcgagcc agccggatgc cgatgacccc ggctgcgtcg
     6301 cgacccatgg gccgcacttc cttctcgttg aagcggccga tcttgccctg cgctgacgcg
     6361 agcaggatgt cgtccgagcc cgacgacacc gcgacccacg cgagctcgtc cttgtcggcc
     6421 acggtgatgg cgcgaatgcc ggtcgagcgc acgcgctcga actgctcgat gggcgtcttc
     6481 ttgatcatgc ccttgcgggt cgccatgacc aggttgtcgc ccttctcgaa gttggggatg
     6541 gtgatgatgg ccaggacgcg ctcattgggt ggttcgagct gcacgccctc gagattcacg
     6601 acgggtatgc ccttgctcgt ccgctcggtc ggcgccggga tcgcgtggac cttggagctg
     6661 aacacgcggc cctggttggt gaagaacagc acccagtcgt gggtcccggc caccagcagg
     6721 tgctcgaccg cgtcctcctc gacggtgcgc gcgccgatga tgcccttgcc cccgcggatc
     6781 tggcgccggt acgtggccag cggctggcgc ttgatgtagc cgcgtttcga gacggtcacg
     6841 acgacatcct cgtcggcgat gaggtcctcg tcggtcagct cgcggtcggc gtcctcctgg
     6901 acgcgcgtct tgcgctcgcc ggaatacttg gccgacatct cgcgcagctc gtccttgatg
     6961 atcgacagga cgcgcgccgg gttggcgagg atgtcctcga gctcggcgat gagcttgatc
     7021 atggcgaggt actcgtcctc gatcttcttg cgctcgagcg cggccaagcg gcgcagctgc
     7081 atctcgagga tggcattggc ctggatctcg gtcagctcga agcggctcat gaggttggcg
     7141 cgcgcggtgt cgacgtcggc cgactcgcgg atcgtcttga tcaccgcgtc gaggtggtcg
     7201 agagcgatct tgaggccctc gaggatgtgc gcccgatcgc gcgcccggcc caggtcgaac
     7261 tcggtccggc gccggacgac ctcgcgccgc cagtcgatgt ggtgctgcag cacgcccttg
     7321 aggggcagcg tctgcggctg gccgtcgacg agggccagca tgttgacgtt gtaggcgagc
     7381 ttcagtgccg tgtgcttgaa caggttgttg agcactttgc gcggcgccgc gtcgcgcttg
     7441 cactcgatga ccagacgcat gccgtcgcga tccgactcat cgcggatgtc ggcgatgccc
     7501 tcgaccttct tgtgctgcac caggtccgcg atcttctcgg ccagcgtggc cttgttgacc
     7561 tggaagggca gctcggtcac gacgatcgcc atgcgctgct ggcgcgtttc ctcgaacgac
     7621 accgtggcct gcatgacgat gcggccgcgg ccgtgggcat acatctcgcg gatcgcgtcg
     7681 aactcctcga cctggcccgt gacgctgttg cgccgcgtgt cgcgccggaa gatcgtggcg
     7741 ccggtcggga agtcagggcc cttgacgatg ccgcacagct cgtcattggt gaggctcggg
     7801 ttctcgatga gcgcgatcgt cgcctcagcg acctcaccca ggttgtgcgg cgggatgttg
     7861 gtcgccatgc cgacggcgat gccggccgag ccgttgacca gcaggttggg cagcttggcc
     7921 ggcagaacgc ttggctgctg gcgcgtgccg tcgtagttct cgacgaagtc caccgtctgc
     7981 ttgtcgatgt cggccagcac ctcggcggcg atcgccgtca tgcgcgcctc ggtgtagcgc
     8041 atcgccgccg gcgggtcgcc atcgactgat ccgaagttgc cctggccatc gatgagcggg
     8101 taccgcaggg agaacgtctg ggccagccgc acgagcgcgt cgtacagcgc gacgtcgccg
     8161 tgcgggtggt acttgcccat cacctcgccg acgatcgcgg cgcacttgcg gtagctcgcc
     8221 gcagatgtga gccccatctc gtgcatcgtg tagaggattc gccgatgaac cggcttcagg
     8281 ccatcgcgca cgtcgggcag cgcacggctg acgatgacgc tcatcgcgta gtcgaggtac
     8341 gagacgcgca gctcgtcctc gatgcggatc gggactacgc gcccttcgtc ggtcatcgct
     8401 gaccgcgacc ctgctggcgc gcgccctgct gttcgagagc cgcgtcggcg aggcgctcat
     8461 agccgacgaa gccgcgtgcc gtttccgacg cttcgctggt ggacggcttg cggccgtcgc
     8521 gccgcacgcc ctcgagccgg gcgcggatct cggccgcgaa tgagccatcc acgagcggcg
     8581 aggtaagcgc gtcgcgtagc acccacgccc tgtggccatc gttgttgcgc agcgcgtccc
     8641 cggcttcggt gcggatcagc tcgcgcacgc cgttgggatc tacctcgaac cacgaacgca
     8701 gggcccacga aagtgccttc tggacgtcag gctccgcatc tccgaggagc gtcctgatga
     8761 gcgtcaaagc gggcgtgcgc gccaggcgag gccgctcgcc acgccccagc tcaaacggca
     8821 gctgggcgag cgtcgcaccg accagccggc gctcccacgg actcgccgcg tagacgagct
     8881 gctccagctc cgcccagcgg tagtgctcgc gcatgatgcc cgcggcgtag agcccggcca
     8941 atgagtcgac gctgatccat tcgtgggccg cgtgggccag cttgcgcatc agctgccagg
     9001 tgcgctccgg gtcgctcggc agcgagcgca gcaggcaggt gtgggcgagg atcgtgaatt
     9061 cgcgttccgg ctcgatcacg agccgctcgg ccagccacag ggcactcgcg tctgaaccct
     9121 gcttgaggct cgccttgagc tggcgggcga tggccgcgag gagcgggtta cggaccccaa
     9181 agacggcccc agtaccgggc gcgacgcgtg cctgctcggc gcggtagggc tcgtcggcga
     9241 ggttggtcag gcccgtgcgg gcgatggcca cgaattcctc gggctgatcg atcagctcgg
     9301 ccagcgtctg acccaggccg cgcgcctcgg gcaggcgctc ggcgaccaac gcctgcgcgc
     9361 ggcgagtgat gtcagacgtc aaggttctgc acctggcgtg cgcgggcctt gatgaagtcg
     9421 cgccgcggct cgaccttctc gcccatcagc gtgctgaaga tgcggtcggc ctcggcagcg
     9481 tccacgatgt cggcccggag cagcgtccgc gtggccgggc tcatggtcgt ctcccagagc
     9541 tgctcggcgt tcatctcgcc gaggcccttg aagcgctgga tggtcaggtt cttggccttg
     9601 aactccttga cgacgcgttc acggtcggcc tcgtccttcg cgtagcgcgt gaccttgccg
     9661 gtgctgacgc ggaacagcgg cggctgggcg atgtacaggt gtccgccctc gatcacgaac
     9721 ggcatgtggc ggtagaagaa ggtcatgagc agcgtgcgga tgtgtgcgcc atccacgtcc
     9781 gcgtcggcca gcaggatgat cttgtggtag cgcaacttgg cgatatcgaa cgtctcgccg
     9841 atgcccgcgc ccagggcgat gatcagcggc cggatcttct cgcttgacag gaccttgtcg
     9901 atgcgcgcct tctcgacgtt gagcatcttg ccgaacatcg gcaggaccgc ctggaagtgg
     9961 cgatcgcggc cctgcttggc cgagccaccg gccgagtcgc cctcgacgat gaacagctcg
    10021 ctccgcgccg ggtcgcgctc ctggcagtca gccagcttgc ccggcagggc gctgccctcg
    10081 agcgccgact tgcggatgac caggtcgcgc gccttgcgtg cggcgtcgcg cgcccgcgcc
    10141 gcgaggagcg acttctcgat gatgcgccgc gcgtcctgcg ggttctcctc gagatactgg
    10201 ttgatgccct cggcaacggc cgcctggacc tggcccttga cctcggcatt gccgagcttg
    10261 gccttggtct ggccctcgaa ctgggggtcg gtcagcttga cgctgatcac cgcggtgagg
    10321 ccctcgcgca cgtcgtcgcc cgacaggttc gcgtcgctgt ccttgagtat cgcgttcttg
    10381 cgcgcgtact cgttgagcga cgacgtcagc gccgcgcgga agccggtcac gtgcgtgccg
    10441 ccgtcgaccg tgttgatgtt gttggcgaac gcgaacacgc tctcggaata cccgtcgttg
    10501 tactggatcg cgacctcgac ctgcgttgag ccgtcgcgcc gctcggcgta gatgggccgc
    10561 ggatggagca cgtcccgatc gcggttcagg tggcgcacga acgagacgag gccgccttcg
    10621 aaatagaagc tcttctcacg tgccggcgtc ggccgctcgt cgacgaactt gatccacagg
    10681 cccttgttga gatacgccga ctcgcgcagc cgctgctcga ccgtctcgaa gctccattcg
    10741 agcgtctcga agacgagcgc gtcgggtttg aaggcggtca gcgtgccgtg gctgccgttg
    10801 ctggcgcgga ccgcgtggac ggagttggtc ggcttgccgc gctcatagtc ctgccgccag
    10861 accttctcgc cgaaggccga ttcgacgtac agccactccg acagggcgtt gaccacgctc
    10921 acacccacgc cgtgcaggcc gcccgacacc ttgtagccgc cgccgccgaa cttgccgccg
    10981 gcatgcagca ccgtgtgcac gacctcgagg gcatccttgc ccgtctgctt ctgcttgcca
    11041 accggcacgc cacggccgtt gtcgcggttg acgacgctgc catcgggctt gatggtcagc
    11101 tcgatgagcg tggcgtggcc cgccatggcc tcatcgatcg agttgtcgac gacctcccag
    11161 atcaggtgat gcaggccgcg cccgtcggtc gagccgatgt acatgcccgg ccgcttgcgg
    11221 accgcctcga ggccctccag gacctggatg ctctcggcgg tgtacgcggc gtccgccgcg
    11281 cccttcgcct tgcgcccgtt gcccgcactc ccccgcgcgg tcggcttggt cagctgatcc
    11341 gtcacgcggc gccaccggtg agccgctcgt acagccgctc gagttcttcc tgggtgtagt
    11401 actcgatcgt gatcttgccc cccttgcggc tggtgctgag cgtgaccttg gttgcgagcg
    11461 cctcgcgcag gccgcgctcc aacctctcga catcggcggg acgcgccgtg gcggagcgcg
    11521 tggacttcgg cttgccctta atcgaacggc gtacgagttc ctctgtctgc cgcaccgaca
    11581 ggccagagga gagtacctga gatagggcat ttgcctgctc cgcggcgtcc ggcaggccgg
    11641 ccagggcacg ggcatgaccc tcggtgatct gacccgcggt cagcgcggcc tgaacgccct
    11701 cgtcgaggtc gagcaggcgc agtgtgttgg ccactgcgga gcgtgatcgg ccgacgcgcg
    11761 cgccgacgtc ctcgtggctc agcccgaact cgtcgaccag ccgccggaaa gcgtgcgcct
    11821 cctcgagcgc gttgagatcg gagcgctgca ggttctcgat gagcgcccac gccagctggg
    11881 cgccaccatc ggcgctgcgc acgagggcgg ggatgcgctc gaggccggcc atctgcgccg
    11941 cgcgcagccg gcgctcgccg gcgatcagct gatagccgtc tgcgctgtgc gtgacgagga
    12001 tcggctggag caccccgtgc tcggcgatgc ttgccgccag gtcggccacg ttcgcctgct
    12061 cgatcccctt gcgtggctga tacgggttgt ggcggatggc cgagatcggt agctcgaccg
    12121 acgcggcggg ctcgtcgcgc tgcgggatga gcgccgcgag gccgcgcccc agtccgccgc
    12181 tgcgccgccc ctccgccgtc acgcgcctac cccggccacc ggcccgcgcg cgccgtggcg
    12241 ctgcagcacc tcctcggcca gcgccgcgta cgcctgggcg ccgcgcgact ccgggctgta
    12301 gcgcacgatc ggccgcccgt acgagggcgc ctcggcaagg cgaactgagc gtggaacgat
    12361 ggtctcgtac accgttccat taaggtgccg gcgcacctct gcggccacgt cggaagacaa
    12421 ggttgtgcgc gcgtcgtaca tcgtgagcag cactccgccc aggcgcagcc gcggattcag
    12481 gttgtcgcgc acaaggtcga cggtggccag gagctgcccg aggccctcga gcgcgtagta
    12541 ctcgcactgg atcggcacca gcacggcgtc ggccgcagtc agggcgttga cggtcaggag
    12601 gccgacggcc ggcgggcagt cgaggattat gaagtcatac gcgtcccgca cctgtgccag
    12661 cgcggcggac aacttgcgct cgcgctggcc cacactgacg agctcgacct ccgctccagc
    12721 gaggtcaagc ccggatggga tcaacgacag cccgtcgatc tgcgtcggct gcacgacgga
    12781 gcgcgtcagc tgcgggtcga cgagcgcggg gtagatggag cttgcaccgc cctggcggtc
    12841 gactccgagg ccgctggtag cgttgccctg tggatcgaga tccacgacca ggacacgccg
    12901 acccgctagc gcgagatagg ccgccaggtt gatgaccgtg gtggtcttgc cgacgccccc
    12961 cttttggttc gcgcaggcga tgacgtcgcc cactcagcga ccgaaggaag agagtgcatt
    13021 cacgtcctca gattctatcg gaaacggccc attcagccat tgtcgggacg cccgctgcac
    13081 gctggatgca ctgccgcttc acgcgcgatt tcacgtgaaa cagccggagg ggcttcgcgc
    13141 gtctgttggg acagcgtttc acgtgaaacg ggcggccgag ggcgcgcgca ggcctctcag
    13201 gaacccgacc tatactcggg ccgtcacgcg cagccagcgc gtctgccgtg cggccagcga
    13261 cactcggagc tcagcttgac cattcctttc gcggcgctct tcacgatcct cgccgcgttg
    13321 ctcgaaacga ccgttctgcc ggagctgccc atcgccggtg cgatcgcgga cctggtcctc
    13381 atctgtgcga cgtgcgctgc aatcatcctg ggcatcgaag acggcctcgc cgcagccttc
    13441 ctgggcggac tgatgatcga tctgctcgtt cccgatcggc cccttggcgc cgcgacactg
    13501 tccctgctgc tggtgctcag cctggccatg atcgttgcgc gcgtgggcgg ccccagtcga
    13561 cgctggctcg cggtagcgat gaccgtgatc ctcacgccgc tgctgcacgg tctgatgacg
    13621 gtcattttgg tcgcgaccga aggcgtgccg ctcgtgttct cacccagcgc cgtgcttgtg
    13681 gccgcgttca tgaacggcgt gatcgcgatc ccggtggcca cgctcttcac cgccatcgag
    13741 caccgcttcg ggaccgtgga gcgcgtcgac tggtgacgca tggctgacta cgtcctgggc
    13801 cggcccgagc cgccgcaaaa ccgtcttggc gtgcgcttcg gcgtgttcgg gttgatcgtc
    13861 gttctcgtca tcggggcctt gacgacgcgc ctcttctaca tgcagggcgt tcagggtggc
    13921 tactacaccg acctggctga ggcgaaccag caccggacgc tgcccatcaa atcggcccgc
    13981 ggcatcatct atgaccgcgc cgggcgcgag ctggcgatca acatcccgtc gtacgtcgtc
    14041 tacatccgcc cggccgacct gcccttcacc cagcgcgacg cggtcgccga cgagctcgga
    14101 aggctgctcg gcatcgatcc cacggagatc atcgccgagg ttgacaggta cgccagccag
    14161 cgctttgagc tggtgcgcat cgcgtccgac atctcgagcg acgtggcccg gatcatcgtg
    14221 gaggaaaacc gcaacctgcc cggcgtggag atcggcatcg aggaccggcg cgagtacgag
    14281 tacggccccc tgctggcgca cgtcctcggg tacgtctcgc caatcagcgc cgatcaactc
    14341 gcgcagctga aggacgaggg ctacctggcc gacgatcgga tcggccagac aggcgtcgag
    14401 gccaggttcg aagacgtcct gcgcggcacc tacggccagg agcaggtcga gcgtgacgcg
    14461 tcaggcagga tcgtgcgcac gatccagatc cttgagcagc cccaggccgg caattcgctc
    14521 gagctgacga tcgatacgga aatccagcgc gaagcgacgg aggccctgca gtgggctacc
    14581 gacatcgtca acctgcagca cggcgtcatc atcgtcgcca atccgcagac cggcgagatc
    14641 ctggccatgg tgtcgctgcc gacttacgac gacaacatgt ttgcgcgcgg catcacgacg
    14701 gccgagtaca agtcactcat cgacaacccc aaccatccac tcgtcaacta cgccatctcc
    14761 gagcagtacc cgcccgggtc cacgtacaag ctcgtcacgg cctctggcgc gctgatggat
    14821 ggcctgttga gcccgaccca gcagctcgag accgcggcct acctggagat cggtccgtac
    14881 cgctactacg actggaaccg gcgcggcttc ggcccgctga acgtgacgca ggcgtacgcc
    14941 atctcaagcg acacgtattt ctaccaggta gccggcatgc tcggcatcga ccgcctcgcg
    15001 tactgggcgg accagtgggg tttcggcaag cgcaccggta ttgacctgcc ggccgaggcc
    15061 aagggcatca tcccgaccaa cgcctggaaa gaggccacgt tcggacagga gatcttcccg
    15121 ggcgaggtct accacgccgg cattggccag ggctacgacg cagttacgcc gctgcagctg
    15181 ctgaacgcct actgcgccct agccaacggc ggcacgctct acaaaccgca gatcgtgcgt
    15241 cgggtgctcg cacccgacgg cacggtcgtg caggacttcc agcccgaggt gctctcaacc
    15301 gtcggcatcg atcccgagat cctgcgcacg atgcgcctgg cggcgcgcga ggtcgtgacg
    15361 acccgccact cgggcaacct ggttgatctg ccgctggtcg tcgcgggcaa gaccggcacc
    15421 gccgagttcg gcacgcgtga cggcgagggc cgactgccct tccactcgtg gttcgtggcc
    15481 ttcgtgccca acttcaccgc cgaccaagcg ggcgatccgg cagcgaccga ttctgagctg
    15541 gcgatcgtcg ccttcgccta tgacgccgtg gtcaaggcca atgccgcgac cgagatggtc
    15601 aagtatttcc tgcaggagca ctacgacctg ggcgtcgacc tgacgcggcc cgacctgatg
    15661 cagggcggca acctgtgggg cggtaactag atgaccgcga tgcgcatcga gcgctcccag
    15721 ctgagcacgc tcggggcgcg cccagaatcc ggcagctggc gctcgtttga catccagctg
    15781 gcggtcgcgg ccctgtcgct cgctgtgatc ggactgctga tggcgtggac gaacagccct
    15841 gacggcccct tgagcgtcgg ctcggtcttc acgcgcggcc tgatgtggtt cgcgatcgcg
    15901 atcatcgcct tcgcggcggt cgccgtgttc gactaccgct ggctgagcac ctttgccgcg
    15961 ccgatctact tcatcaacat cggcttgctc atcctcacgc tgatcatcgg cagcacgatc
    16021 aacggcgctc agcgctggct ctcgatcggt ggcctgacct tccagatctc agagatctcg
    16081 aaggtcgtga tgatcggcgt cctggccacc ttcctggaag cgcgcaagaa caacctgggc
    16141 ggcctttcga cgctcatcgg cgccggactg ctcatcgcac cgcccttcgt gcttgtcatg
    16201 atccagcccg accttggcag cgcactcgtg ctcgtcgcga tcgctgcggg cgcgctcttc
    16261 ctgagcggcg cgagcctgcg ctggatgggt ctcgccgcgg cctcggtcgt ggccgcggtg
    16321 cccgtcatct ggtcgatcct gcaggactac cagcggcgcc gcctgctgtc gttcctcgac
    16381 cccacgtcgg atccccaggg cgccggcttc caggtcatcc aggcgcagat agccgtcggc
    16441 tcaggcgggc tgttcggcaa gggtctgacc aacggcagcc acagtgcgag cgacctgttg
    16501 ccggtgcagt cgaccgactt cgccttcgcc gtgctactcg aggagctcgg gttcttcggc
    16561 ggggtcgtgg tgttcctgct gttcatatgg ctgatctggc gcgtgctgct tgtcggctgg
    16621 cgttccggat cggtgttcgg catcgccttc gcaggtggtg tcgcgtcgat gatcgtgttc
    16681 cagatcctgg tcaacgcggg catgatcgcc ggcatcatgc cggtgaccgg cattcccttg
    16741 ccgttcatca cccacggcgg ggcgtcactg gtgagcacgg ccatcgcgct cgggctcatg
    16801 cagagcatcg ccatgcgctc ggagcagccg cggccgtcgt ggtagcgcgg ccgtcctggt
    16861 agcgccctga acgactaccc gctaagcccc cttagcggcg gagcaatgcc tcgatctcgg
    16921 cgcggtacat acgcccggcc ggcgcgcgca tgagccattc gagaaactcg ggatcgcggc
    16981 gcttgatctg gccgagcgtc cagccgctgt aacggccaaa gtcgatcagg ctgccgctgg
    17041 gcggaccgac cgccgggccg gcaccgcccg tggccacgtc ggacgcgtga cggacaagcc
    17101 ggtcgctggc gcgccgatcg cgccgtgcgg ccagccactc gtcgtagcgg cgccgcttct
    17161 gcgggtcgcg cagtgtctgg taggcctcgt tgatggcggc catgcgttgc gccgcctcgt
    17221 ggctcgaatc cacgtcaggg tggtagcgct gggcgagctt gcgatagacc gtgctgatga
    17281 tctcctggtc ggcgctctcg gccagcatca gtaccttgta gtgcgtcgtc cgcgggtcgg
    17341 ggttcataac ttggcgtcat cgtcgtcctc caacgtgagt gtggataggt gacgatgtgc
    17401 ggggacgcga ccggtacctt catcgccgca acttccctgc gggcggtagc tgcgcggtcc
    17461 cactcttggc tcgtagcagg cccgtgttgt atccttctcg ttcgcgccca aagcgccccc
    17521 aaaggtccgt ccattcgccc atcaaccgcg ccttccggag agtcatgtac gccgtcatcg
    17581 aaacaggtgg aaagcagtac cgcgtcgagc tcggtagcga gttcgccgtg gagcggctcg
    17641 agggcacccc gggcgatacg atcaagttcg agcgtgtcct gctcgtcgct gacggcgaca
    17701 aggcgagcat cggcactccg gtcgttgatg aagcgctcgt gaccgcgagc ctggttcgcc
    17761 aggaccgcgg cgaaaagatc atcgtcttca agtaccgccc caaggcgcgt catcgttcga
    17821 agaacggcca tcgccaggag cagacgatcg tccgcatcgc tgacatcacg ctgggtggcc
    17881 gcagcgcggc gaccgaggcg gaggccgagc agaaggaagc gtccaaggcg aaggccaagg
    17941 ccgccgccga ggctgagaag aaggccgctg ccgatcaggc gctcgcagcc aagctcgaga
    18001 aggacgccca ggcgaaggct gccgacgcga aggctgccgc ggccaaggcc gataagccgg
    18061 ctgagaccaa ggcaaccaag gcaaagtccg agcccaaggc caaggcagct gccacaaagg
    18121 caagtgcacc caaggccgcc aagccggccg ccaagaagac tgaaaccgcg ccggccaaga
    18181 agccgcgcgc caagaaggac gagtaagcga catggcacac aagaaagcgg gctcaagctc
    18241 gaagaacggc cgcgacagcg tcggccagcg tctcggcgtg aaggccggcg acggccagct
    18301 cgtgtcggcc ggttcgatca tcgtccgcca gcgcggcatg accttcctgg ccggccccgg
    18361 tgcgggcctg ggcaaggact acacggtgtt cgcgctgacc gacgggcgcg tcaagttcga
    18421 gcatgagaag cgcgacaaga agcgcgtccg catcgtggcc gagacagccc agacggctca
    18481 gaccgcccag gcaggcgcca aggcatgaag cccgacattc accccaagta ccacgccgcc
    18541 aaggtcgtgt gcgcctcgtg ccacacggag ttcgaggttg gctcgaccca gcagacgctg
    18601 catgtcgaca tctgctcaaa ctgccacccg ttcttcacgg gcaagcagac gatcctcgat
    18661 cgcgctggcc aggtcgagcg cttccagaag cgcctcgagc gcacggcgcg gctctgatcg
    18721 cccggcccga ctgggctcgg gcctaaactt tctcgcacat ggcgcgaact gatttcggtg
    18781 gccaggccct gctagagggc gtcctgatgc gcggccgcaa ctcgatcggc atcgcactgc
    18841 gccaccccga cggctccatc gtgtccacgg ccgagccgct caactccatc ctgcaccgca
    18901 accgctttgc ccgcgcacca ttcttccgcg gcctcgtgct gttgtacgag acgctcgtca
    18961 tcggcacgcg ctggctgatg cgctcgggct cggtccaggc agagggcgag ggccaggagc
    19021 tgggcaaggg cacgatcgcg ctgacgctga tcgtcacgct gggcatggcg atcggtctct
    19081 tcgtcctgct gccgctgttc ctcgctggtg cagccacgaa cgcagtcgcc gggccgggtc
    19141 agacgctcct cgcgcacttg gtcgaggggc tgatccgcgt cgtgatcttc gtcggctacc
    19201 tgctcctggt gagccggttc ggcgatatcc aacgcacgtt ccagtaccac ggcgccgagc
    19261 acatgacgat ccacgccctg gagcacgagg atccgctgac cgtcgagcag gtgcgcaagt
    19321 acccgaccgc gcaccagcgc tgcggcacgg agttcctggt catcttcatc atcctgtcga
    19381 tcctgatgtt cagcctgctc gcgggccaaa gcctggtggt gagcatcgtg ggccgcgtgc
    19441 tgctcgtgcc ggtgatcgcg tcgctcgcat acgagctgtt gcgcttcggc gctcgctacc
    19501 gccaccactg gtggattcgc tggctgtacc tgcccggcat ctggctgcag ggcatcacta
    19561 cgcgccagcc cgacgacgga atgatcgagg tcgccatcgc gtcgatgcgt gaggccctcg
    19621 ccgcaaacgg cgaagaggcg cccgccggca gcctcgatcc cttacgccgg ccgatcccga
    19681 gcggagtcga ggagacagcc ggctcggcga accaggccag ctgatgggcc tcgacgagcg
    19741 cctcgacgcg ctcgagcgcc gtttgatggg gatcgaatcg gagtgggcgc ggcccgaggt
    19801 cgcgtctgac cctgcgcagc ggcgcgccct gggccgcgag cagtcgcaga tcgagccgat
    19861 cgtccatacc taccggcggc tgcgcctggt gcgggagcag ctcgcgtccg cgcacgcagc
    19921 ggagagcgat cccgagttgc gcgacctggc ccgtgccgag atcgaccagg cggagatcga
    19981 ggagcgggtg ctgaccgagg agttgcgcgt cctgctcctg ccgcgcgacc ccaacgacga
    20041 taagaacgtc atcgtcgaga tccgtgccgg cactggcggt gaggaggccg cgctattcgc
    20101 cgcgcaactt ttccgcatgt acaagcgcta cgccgagcgt catcgctggc agagcgagct
    20161 gatctcggcc aacgagaccg gcatcggtgg cctgcgcgag gtcatattcg aggtgcgcgg
    20221 ccagggcgcg tacagccgtc tcaagttcga gggcggtgtg caccgcgttc agcgcgtgcc
    20281 ggaaaccgag tcgagcggcc gcatccacac ctcgacggtg accgtcgcgg tgctgcccga
    20341 ggccgacgag gtcgaggtcc agatcgatga caaggacctg cgcatcgatg tctatcgctc
    20401 gcaggggccg ggtggccaga gcgtcaacac gaccgactcg gccgtgcgca tcacgcacat
    20461 cccgtccggc atcgtcgtgg ccatccagga cgagaagagc cagcacaaga accgcgccaa
    20521 ggcgatgagc gtgctgcgcg cgcggttgct cgagcaggag cagcgaaaag cgcatgaagc
    20581 tgaagcggaa gcgcgccgct cgatggtcgg gtcgggcgag cggtcggaga agattcgcac
    20641 gtacaacttt ccgcaggacc gggtcaccga tcaccgcatc ggcgtggacc tgcacaacct
    20701 gcccaacgtg ctcgacggcg acctggatcg gctgctcgac gagctgatct cgaccgacca
    20761 ggcgcagcgc ctggcgcacg tcgaggaggc atcgacggcc ggggtcgctt gaaagtctga
    20821 tcgggggatc gattggccaa gacagtcagc gccgagaacg ctccacgcga catcttcgag
    20881 gagccgctcg ctcccggcgc gcccgcgccc ccggtcgtcc gcgcgacaga tctcacgggc
    20941 gtacatgtcc tcaaggcagg cgacatgttc atgctcaaca acgcccatgg cgacgtgcgg
    21001 cccgacggtc gcgggctggg cctgttcctg ggtgacacgc gcttcctgtc gacctacgac
    21061 ctcgtgctga acggcactca tccagtcgtg ctgcgggctg gccccgcggc ggcgcagcac
    21121 agtgtcatcc agatggccaa tccggacctg ctcgagcagg gcggcgacgt cagcgaggag
    21181 gttgtgctgc gccgccactc gctgggcgtc gtgcgcgacc gcaccgtcag cgaccgcctg
    21241 aaagaacggg tgacggtcac gaactacacg accctgcccc agcgcgcgcg cctgaccctg
    21301 atgctcgacg ccgactacgc ggatatcttc gagctgcgcg ggctcgtgcg tgagcgacgg
    21361 ggcgaccgcc tggccaacga gtggtcaccc gacggcctga cgttcacata ccgcggcctg
    21421 gacaggatcg tgcgccgtac ggttatcagc ctcgagccgc acatgcaccg cgcggcgaca
    21481 acggttccgg acgcggagct cgacaccgcc ggaaccacgc tcgagctcga ttggctgatc
    21541 gagccggggc gcagcgagac actgaacatc gaggttcgcg cggagatccg cgacgactcg
    21601 ccgccgcaca cgccaccccg accgtggaca gtgggggcca cgatcgagac gaaccacgtc
    21661 ctggccgaac gcgcgctcga gcggtcgcgc gccgatctga acctgctgat caacgacgga
    21721 cccgcagccg acgagcgcta catcgctgcc ggcgtgccgt ggttcagctg cctgttcggg
    21781 cgcgactcga tcatcaccag cctgcagctg ctcgccgtgc ggccggagat cgccgcgacg
    21841 acgctgtcgg tcctggcgcg cctccaggcg acggaggtcg acgagttccg cgacgctgcg
    21901 ccgggcaaga tcctgcacga gctgcgcacg ggcgagctgg cgcggaccga tgagatcccg
    21961 catacgccct actacggcac ggtcgacgcc acgccgctgt ggctgatgct gctcgatgag
    22021 tacgaacgct ggaccggcga ctcggcgctc gtcgaccagc tgtggccgaa cgccctggcg
    22081 gcactcggct ggctcgaccg gtacggcgac ctcgatggcg acggcttcat cgaataccag
    22141 cgccaatccg agcgcggact ggtcaaccag ggctggaagg actcggctga ctcgaaccgc
    22201 aaccgcgatg gcacgctcgc cgaaggcccg atcgcgctgg tcgaggtcca ggcctacgcc
    22261 ttcgccgcgc ggcggggcat ggcgcggctt gcgcgcaggc gcggagacga ggagctggcc
    22321 gcgctgcagg acgccgcggc cgaggcgctg cgtcagcgct tcgagaaggc cttctggatg
    22381 gaggacgtgg gcacgtacgc cattgcgctc gatggcgcca agcgccaggt aacgggcatc
    22441 gcctcaaatg ccggccatgc cctgtggacg ggcatcgccg acccgcagcg tgccgcgcgg
    22501 acggcggagg tgctcacagg acgcgggatg tggagtggct ggggaattcg cacgctgtcc
    22561 gcggagacca tcggttacaa cccgatcggc taccacttgg gcacgatctg gccgcacgac
    22621 aacgccatct gcgcggccgg atttgcgcgt tacggcctgt ccgaggagac gcgcctcgtc
    22681 gcgggtgcgc tattcgaggc gaccgcgcac ttccgcgagg cgcgcctgcc cgagctgttc
    22741 tgcggcttcg accgcgcgac atcgccgctg ccggtgccct accccgtcgc ctgctcaccc
    22801 caggcgtggg ccgcgggctc gctgtaccac ctcatgtccg cgacccttgg tttccggccc
    22861 gacgcgcgcg cccacaggct cgagctggtg cgccctgcat tgccgacgtg gctgcccagc
    22921 ctgcgcgtgc gtggcctgag agtgggcgac gcgcatgttg acctggagtt cggcgcccag
    22981 gagcagtcca tctcagtcga ggtcctgcgg cgtacggcgg acctggacgt cgtagttcgg
    23041 ctttgacgac gaccgtcggc accctgctcc gcgaggccgg tcagaagctg acagaggctg
    23101 ggtccgagac gccgcggctc gacgccgagc tcttgctcgg tcacgtgctt ggagtcggcc
    23161 gggcgactct gctcgcggag cctgaatcgc aggtttcaca ggcccagtcg gcggtcttcg
    23221 ccggtctgat cgaacgccgt gcaagaggcg agcccgtggc ctacctgcgc ggcctgaagg
    23281 agttctacgg cctcgcgttg agcgtggacg agcgcgccct gatcccgcgg ccggaaacgg
    23341 agacggtggt cgagcgagcg ctggccctga ttgccgagcg cttgacgagc tctccccgac
    23401 cggccgacgc gcagccgctc ctggtgtggg atgtcggcac cggcagtggc gcaatcccgg
    23461 tcgcgctggc cgtcgagtgc cgccggcgcg gctacggccg cgacgtcgcg ttccgggcga
    23521 cggacgtgtc tgcggatgcg ctggcgctgg ccaccgagaa cgccgtcgcc cacggcgtcg
    23581 ccgacaggat cgccttcgcg gtggccgacc tggctgatct gcccgacgcg gagcccgtcg
    23641 acatcctgac tgccaatctg ccttacatcc gttccgacga cttgccgcag ctgccgattg
    23701 ccgcgagcta tgagccgacc gtttcactcg acggcggcgt agacggccta acgttagtta
    23761 ggcaaatggt cgcccagcta ccaggcgtcg tcgtgcccgg cggagtggcg ctgctcgaga
    23821 tcggcagcga tcaggccgac gccctggccg cgctcgcacc cacgggctgg gaggtctctg
    23881 ttcacgacga tctggccgcg tggccgcgcg tcatcgagct gacccggccg gcgagcgcgt
    23941 gatggaggtc gttcgctcag acgagccggg cgcgctcgag cgcgcggccg gggcgctcgg
    24001 tcggggagag gtgatcgcga tcccgaccga gaccgtctac ggcctggcgg ccctgccgcg
    24061 cgccgggagc gtggagcacc tcatcgcggc caagcagcgc tcgaccgaaa agggcatcca
    24121 gctgctggtc gactcgctcg aacaggtacg agcggtatgc atggtgccgc ccgccgccca
    24181 gaagctcgcg gacgccttct ggccgggcgg cctgacgatc gtgcttgatc gccgcgcgga
    24241 ctcggacctg ccgctgctcc tgggtggcgg ccgcgcgacg ctgggcgtgc gcctgcccga
    24301 ccatccgctg ccgcgcgagc ttgcgcggat gctcgggccg ctcgccgcga gctcggccaa
    24361 cgtcaccggc cggccgccgg ccacgaccgc gcacatggtc atcgaatccc tgtcggatgt
    24421 cgtgagcctg atcgtcgacg acggtccggt gcggggcggg gtgtcatcga cggtggtcga
    24481 ctgctccggg tcggccgccc aggcgccggt cgtgctgcgc gaaggcgcga tcgccggctc
    24541 ggagatcttc gccgcgctcg gtcaggaata cacttcgcgt tgagcgccgc acgctcattt
    24601 gattgagtgc gcacccgacc tgacctggag gccgtgtctt tgagcgtcag cacgacgtcg
    24661 cgcatcgcct gggccccgct cgccgaagtg gatcctgatc tctggggcgc gatgcaacgc
    24721 gagcgcgaac ggcagcgctg gaacattgag ctcatcgcga gcgagaacta cgcttttgcc
    24781 gcggtcctgg aggcgcaggg ctcgtggctg accaacaagt acgcggaggg ccagccgggc
    24841 aagcgctatt acggcggttg cgagttcgtc gacgaggtcg aggagctcgc cgagcagcgt
    24901 gccctcgcgc tgttccccgg cgccgagcac gtcaatgtcc agcctcattc cggcgcgcag
    24961 gccaatatgg ccgcttacgt gagcgtgctc gagatgggcg atcgcgtgct gggcatgaac
    25021 ctggcccacg gtggtcatct gacgcacggc tcgccggtca acttcagcgg caagtggttc
    25081 gagatccacg cctatggcgt gcgcgaggac gatgcccgca tcgactacga cgcgctcgag
    25141 aagcaggcgc gcgaggtcaa gcccaagttg gttgtcgctg gcgcgagtgc ctacccacgc
    25201 gtcatcgatt tcgagcgcat cgcccagatc gcccacggcg tggacgcgct gttcttcgtg
    25261 gacatggcgc acatcgccgg gctcgtggcg gcgggccagc acccgagccc cttcccgcac
    25321 gccgacctcg tgacgacgac gacccacaag acgctgcgcg ggccgcgcgg cggcctggtg
    25381 ttctgccgcg cagacctggc caagcaggtc gacaagacgg ttttccccgg attgcagggc
    25441 ggcccgctga tgcatgtcat cgccgcgaag gcggtcgcgc tgcatctcgc gagcaccgac
    25501 gagttccgat ccgacatgaa gcacaccgtc gagaacgcca agctgctcgc gacgaccctc
    25561 gccgacaacg gcgcggccgt cgtctccgga ggcacggaca accacctgat gctcgttgac
    25621 gtgacgccgc tcggcgtgac cgggcgcgag gcggagacgc tgctcgacga aatcggcatc
    25681 acggtcaaca agaacgcgat ccccttcgac aagaacccgc ccaacatcgc gtcgggcatt
    25741 cgcgtcggca cgccggcgac cacgacgcgc ggcttcggtc ccgacgagat gcgcgagatt
    25801 ggccagctca tcgtgaaggc catccagggc cggaacgatg agtccgccag ccagctgatc
    25861 aagaccagcg tgcgtgagat gtgcgcccgc ttcccggtcc cgggcctgcc ggcggagtga
    25921 gcccggacgc gctcggcgcg ctgccatacg tcattgccgc gctgatcgcg acgactgtcg
    25981 ccagcctgct gctgacgccg gtcgcgattc gacttgcgcc ccgcctgggg gcgatcgatg
    26041 tcccggattc ggagcgccgc attcacagcc ggcctatccc gcgcacgggc ggactggcgg
    26101 tcgcgctcag ctttctcggc gtcggcgcgc tcggactgct ggccaaccag gtcttccacc
    26161 tggcattgcc cggacggccg gtgcgggcca gcgagctggc ggcactgttc atcggcgttg
    26221 ccatcggcgc cggcttcggc tacctcgatg atcgcctgca gctcaaggcg cgctggcagt
    26281 tcatcgccca gttcgtcctc gccggcatcg cgctcgcggg aggcgtgctg atcaaccgcc
    26341 tgcccgagcc gcttgggccc atcaccgacg ctgcggcgat ggccgcgacg acgatctgga
    26401 tcgtgggcat gctcaacagc gtcaatttcg tggacggcct ggacgggctg ctggccggca
    26461 tttcgctgat cgcggtgacg accctcggca tcaactcgct cttcgccgtg tccattcagc
    26521 cggtcgtcgc ggtgctgtgc gctctactgg ccggggcgct cctgggcttc ctgccctaca
    26581 acttccatcc cgcccgcgtg ttcatcggca catccggagt ctttgccgtg ggctacgcgc
    26641 tggccgtgtt gtctgtgctc ggtacggcca aggtcgcgct ggcgctgctc gtgttgggcg
    26701 tgccgatcat cgacaccttc tggatcatcc tgcgccgtgt gtcagcgggt cgctcgccgt
    26761 tctccgcaga tcgcggccac ttccaccaca ggctgctcga ccttggcctg tcgcaccgcc
    26821 aggcggtcct cctgatctac ctcatctgtt ccatcctggc ggtcctcagc ctggccctgc
    26881 ccagcggcgg ccagatcacg gtattcgtgc tcgttgtcct gggcgggggg gcgctgctgt
    26941 atctgctcac ccgccgcgcg cgcgaggcgc tcgatcggtc gagctacgag gacgaagggc
    27001 caggcgcctc gtcggggaac gagtgacccg ctgctatact cccgccgcgc taccgggtag
    27061 ttgatgaacg accttggccg ggcgactgcg tatctcgctc tcttctcaga gatcgcgatc
    27121 gtcctgctgg tcacgacgct gctcggtgtg ctctcgggct actggctcga tcagcgtcta
    27181 gggacgctac ccatcctgtc ggtcgcgggc atgcttgccg gcttcggact gggcggagtg
    27241 ggggtatatc gactgatcgt gcgcttcctc gctcggttcg agtgagcgcc gagcggccaa
    27301 cggggagaca gtgacagaca cgccgccgca caagggtgcg atgacacccg cggacaagcc
    27361 gactgaagcc gaggcgcgga tgcgcgaggc gcttgggccc gagatggcca gcgacgccgt
    27421 cggggccgcc gctgccgtcg cgtccaagcc gcgctcgaac acccggcgct ggatcttcct
    27481 ggcgatcgtc gccatcgtcg tcctgcaggt cggggccgcc atcttcttcc cgccgaccgg
    27541 ccccgacggc aagccctatc aatacccggc ggacggcatg atcggtgcca acatcgaact
    27601 gcctgcgccc gaggtcgtct tcgacccgac acctgaaaca caccgcgaca cgggcctcgt
    27661 gaccttcgac attaccgtgt cgaacacgct cttcaccggc tggatcgtga tgatcctgat
    27721 cgtcgcggtc gccttcctca tgtcacgggc ggtcaaggtc gtacccacca gggcgcagaa
    27781 cgcgctcgag tacgtctacg agggtctggc caacttcgcg acctcgctcg gcggacggca
    27841 ggcgcggcgc tacgtgccga tcttcgccgc gttcttcctg ttcatcctgc tgtccaactg
    27901 gagcggcctg gtgccgccgg tcggacgagt ccattggctg cgcgcgccga cgagcgacgt
    27961 gaacacgacc atcggcctgg cacttttcag cttcggcatg ttccacgtgg agggcatccg
    28021 cgcgcttggt ttgcggcgtt acctgggcaa gttcttcagc ctgcgtgcgt tccgcactga
    28081 gggggtgtcg gccggcctca tcgcgctctt tgtcgggatc atcgagttcc tgctcgagtt
    28141 catcaagccg gtcacgctgg cgatgcgact cttcggcaac atctacggtg gtgaggtcgc
    28201 gctgggcgtg atcaccggcc tgacgattgc gctgatcccg gcagcaatgg tcgggctcga
    28261 gttcctgctc aacttcatgc aggccctgat cttctcggtc ctgaccctga tgttcactat
    28321 catcgccacg gaggcgcacg acgaccacgc ggagcacgag gctccggagg gcaacatcgc
    28381 gcccgacatg gccggcgccc atccggcgcc cgctcactga ccaagtccaa caaacagccc
    28441 ggtaagtcgc cgggcgctac ggaggtagag agaacgagat ggaactcatt ggtgcaggtc
    28501 tcgccgcgct gggcgtcatc ggccccggca tcggcgtcgg catcctgact ggcctgtcga
    28561 cgagcgcaat cggccgcaac cccgatgctg ccgcgcaaat ccgggccacg ttcatcatcg
    28621 gcgcggcctt cgccgaggga ctgggcgtgc tcgcagtcgt cgtcggcctg ctgtgcatct
    28681 tcctgcgcta gcccgcgcag gaaatttctg tcgtgccagt gttcgcgggc gccggaggcg
    28741 tactagaccc gatcctgcgc ctcttcgctg aagaggccga ggtcggcttc cagctcaacc
    28801 tgttctggat catcgcccag gcaggctcgt tcctgctgtt cttcgtgatc ctgtatttcg
    28861 ccgcgttcaa gcgcataggc ggggtgctcg aggagcggcg ttcgaagatc gagcagggcc
    28921 tgcgcgatgc cgacgatgcg cgcaaggagc gtgagcaggc cgccagcgag cgcctcgcgg
    28981 tcctggccca ggcgcgcaac gaggcggagg acatactcag ccgggcacag aaggtcgcgg
    29041 atgagtcgcg cgaacgcgac atggccgaga cgcgggcgca gctcgagcag ctgcgcaccc
    29101 aggccgcggc ggacatcgcg tccgagaagg aacgcgccct ggccgacgtc cgttctcagg
    29161 tcgccgagct cgcgctcgcg gccgcgtcgc gcgtcgtcgg cgagacgatg accggcgagc
    29221 gcgagaagcg tctcgtcaac gagttcctga ccgaggttgg caagaagtag ccatggccgg
    29281 gacacgcagg agcagcaccg cgcggcgcta tgccgaggcc gtattcgaga tcgccgagcg
    29341 cgacgacacg gttgaacgct ggctgacgca gctgaacgat gtcgccgagg ccgtcagtcg
    29401 caaggaggtc gtgcgccggc tcgaggatcc gcatgtgccg ctcgaggcgc gggccaaggc
    29461 tctgcacgac gttgccggcg ctcagatgct gccccagatg agcaacctcc tggggctcgt
    29521 gttgcgccgc cgccggctcg acacgctgcc tcagattgcg cgcgagttca agcgtctcta
    29581 caaccgccgc gcgggcgtcg tcgaggcgac agccacgagt gccctgaagc tcgatgccca
    29641 agaggtcgcc gatctcacgg cccgcctcga aaacatgacc ggcaagaaga tcgagctgag
    29701 cacagcagtc gacgcgaaca tcctcggcgg cattcaggtc cgcatcggcg acacgctgta
    29761 tgacggcagc gtgcgcggcc ggctcgagcg gctgcgcgcg cgcctcgagt cgggtgcgct
    29821 caccgcgtga tcccgctgaa ttagacagac atcgaaccgc acgagtaacg gagaaaacct
    29881 gcgatggcca tccggtccga cgaaatcacc acgatcatca aatcggcgat cgaccagttc
    29941 gacgccggcg tcgagacgcg ctcggtcggc accgtggtgg aggtcggaga cggcatcgcc
    30001 caggtctacg gcctggaagg tgccctggcg tcggagctgc tcgagtttcc gggcggggta
    30061 atgggcatgg cgctcaacct cgaggaagag acggtcggcg cggtcatcct gggcgacttc
    30121 aagcagatca aggaaggcga ccaggtcaag accacgggcc gcgtggtcga ggtgcctgtc
    30181 ggcgcagcac tcattggccg cgtggtcgac ccactcggcc ggccgctcga tgacaagggg
    30241 ccgatcaacg ccgccgcgac ccgacccgtc gagcggatcg cgcccggcgt catcgtgcgc
    30301 aagagcgtgg atacgcccgt ccagacgggc gtcaaagcga tcgacgcgct catcccgatt
    30361 ggccgcggcc agcgcgagct gatcatcggc gaccgccaga cgggcaagac ggcgatcgcc
    30421 ctcgacacga tcatcaacca gaagggcaaa ggcctggtct gcatttacgt cgccatcggc
    30481 cagaagctct cgactgtggc caagacggtg gccgtgctgg agcagtacgg cgcgatggag
    30541 cacaccatcg ttgtcgtggc cggcgccgag gatcccgcgc cggttcagta catcgccccg
    30601 tatgccggcg cggccatggg cgaggagatc atggaaaacg gcgttgagtt gaacggcacg
    30661 ctcgtcaagg acgcgctgtg cgtctacgac gacctgacca agcacgcctg ggcttatcgc
    30721 gagatgtcgc tcctgctgcg ccggccgccg ggccgcgagg cctacccggg tgacgtgttc
    30781 tatctccact cgcgcctcct cgagcgtgct gcgcggctga acgaggagaa cggcggcggc
    30841 tcgctcacgg cgctgccgat gatcgagacg caggccaacg acgtgtcggc gtacatcccg
    30901 accaacgtca tctccatcac cgacggccag atctatctcg agtccgacct gttcaacgcc
    30961 ggccagcggc cagcgctgaa cgtcggcatc agcgtgtcgc gcgtgggttc ggctgcacag
    31021 accaaggcca tgaagaaggt cgccggcccc ttgaagctcg acctggcgca gtaccgcgag
    31081 ctggccgcgt tcgcccagtt cgcgtccgac ctcgacaagg cgacgcgcga ccagctgacg
    31141 cgcggcgaga agacgtccga ggtgctcaag cagccgcagt acgagccgct cgccgtcgag
    31201 aagcaggtgg ccatcctgtg ggtcgtgacc aatggttacc tcgacgacgt gcccacgccg
    31261 cgcatccgtg actttgagca gcgcttctac cgcttcctgg agaccgagca cccggcggtg
    31321 ctcgacaccc tgggcaagaa gcctgaactc accgacgatg tcgtcagcgg cctcaagagc
    31381 gcaaccgaag cgttcaaaca aacgttcgtt agttaacaga gccggagaag tagcggcaca
    31441 tgcccagtca gcgcgagatc cggcgccgga tctcgtcggt gcgcaacatc aagcagatca
    31501 cgcgcgcgat gcagttcgtt gccgcgtcga agctcaagcg cgcccaggac gccactctcc
    31561 agtcgcggcc atacgccgac aagatcgacg aggtcctcgc ggacctggcg gccgtgctgg
    31621 gcgccgacga ccacccgctg ctcgcccggc gcgagggtgg cacacgggcg atcgtcctgc
    31681 tcacgacaga ccgcggcctc gcgggctcgc tcaataccaa caccatccgc tacaccgccg
    31741 agctgatcac gaaggaaacg gacgagatgc gcatggtcac cgtgggccgc aagggccacg
    31801 cgtcgatgcg ccgggcccgc gttcccattg cggccacgtt cgagggctac ggcgaccggc
    31861 ccaccttctc gtccgtcgtg ccgctggccc gtctgctgac cgacgattac ctggcgggca
    31921 cgtattcaga gatcgacctc gtctacccgc gcttcgtttc gacgctcgtc cagcggccgc
    31981 acctcgatcg gctgctgccc gttgagcccg cgagcgacgc ggacgaagga attcccggca
    32041 accagttcat tttcgaaccg aacgccgacg ccgtgctgcg ccagctgctg ccgcgctacg
    32101 tggcgacgcg cgtctaccag gcggtgctcg agctgacggc gagcgagcag tcggcgcgta
    32161 tggtcgccat gaagaacgcg acggagaacg cgcaggagct gatcgaggac ttgacgctca
    32221 cctacaacaa ggttcgccag tcgaacatca cccgagaaat gatcgagatc gccaccggcg
    32281 cgagcgcgtc gtgaccccag gaggagcatt cacgccatgg cagtagccgc cccaagcaag
    32341 aaaggcgttc ggaccggccg ggtcatcgcc atcacgggcc cagtggtcga catcgagttc
    32401 ccgcaggatc acctgccagg gatcttcaac gccgtcgaga tcatgcgcga gggccttgag
    32461 ccgctcgtag tcgaggtcca gcagcacctc ggcaacaact gggtgcgcac cgtggccatg
    32521 accacgaccg acggcctggc gcgtggcgcc gaggcggtcg acaccggtgg gccgatctcc
    32581 gtgccggtgg gtccggccac ccttggccgc gtattcgatg tcctgggccg cccgatcgac
    32641 aacaagggcc cggtcgatgc gcagcagagg tacccgattc atcgcgcggc gccgtccttc
    32701 gaggaccaga ccaccgctac agaagtcttc gagaccggtc tcaaggcgat cgacctcatc
    32761 gcgccctttg ccaagggcgg caaggccagc gtcttcggcg gtgcaggcgt gggcaagacc
    32821 gtcgtcatcc aggagttgat ccgcaacgtt gccgaggagc acggcggcta ctcggtcttc
    32881 gccggcgtgg gcgagcgctc gcgcgagggc aacgacctgt tgcacgagat gaccgaatcg
    32941 ggcgtcatcg ccaagaccgt catggtcttc ggccagatga acgagccgcc gggcgcgcgt
    33001 ctgcgtgtcg gtctcagcgg cgtcaccttc gccgaatatt tccgcgacga ggggcgcgat
    33061 gtgctcctct tcatcgacaa catcttccgt ttcacgcagg ctggctcaga ggtgtcggcg
    33121 ctgctgggcc ggatgccgtc ggcggtcggc taccagccca atctggcgac ggagatggcc
    33181 ggcctgcagg agcggatcac ctcgaccaag aagggctcga tcacgtcgct ccaggcgatc
    33241 ttcgtgcccg ccgacgacta caccgacccg gcaccggcca cgacctttgc ccatcttgac
    33301 tcgtcgatcc gcctcgagcg ctacctgacc gagctgggga tctacccgtc ggtcgacccg
    33361 ctgacctcga cctcgcgcgt gctcgacccg aacatcgtcg gtcaggagca ctacgagacc
    33421 gcgcgcgaga cgcagcgtgt cctgcagagc tatcgcgacc tgcaggacat catcgccatc
    33481 ctgggcgtcg acgagctctc agaggagcag aagatcacgg tcgcccgcgc gcggcgcctg
    33541 cagcgcttca tgagccagcc catgttcgtg gccgaggtct tcaccggccg gcccgggcgc
    33601 tacgtcgcaa tcaaggacac cgtcgcgtca ttcaaggaga tcctcgaagg caaggcggac
    33661 gacctgcccg agcaggcctt cttcctcgcc ggaacccttg atgacgtgcg cgagaacgcc
    33721 aagaagctga acgcctagat gccactgctg ctcgagatcg tcaccccgga gaggcaggtc
    33781 ttctccgagg aggtcgattc ggtcgtctgc ccgggtatcg aaggtgagct cggcgtgctg
    33841 ccgcaccacg cgccgctcct gaccacgctg ggcgtgggcg agctgcgcat ccggcgcggc
    33901 ggtagcgagg agtacttcgc catcgcgggc ggcttcctcc aggtgcggcc agacaaggtc
    33961 gtcgtcatgg ccgagacagc cgacctggcg agcgatatcg acctcgagaa ggcgacggcg
    34021 gcacggcgcg aggccgagca ggcactcgcc gagggcttcg aggagccggc cgatctcgct
    34081 cgtgcccggg cgtccctgca gcgtgcgctg ctgcgcatcc gcgtcgccga gcggcaccgc
    34141 cgggagggtc cgcgctcgcg cggctaggag atacgtggcc gcaggaaccc atggctgatc
    34201 gtctgttcca ctgggaatac caagagctcc cggttgagcg acaggctttt cgggtaacag
    34261 gcggccgccc gttgcgcggc aaggtgcaga tcagcggcag caagaacgcc gcgctcaaga
    34321 tgctcgccgc ggcgacgctg accggcgagc gctgccgctt cacgaacgtg cccgagatcg
    34381 aggacgtgcg cgtcatggcc gacgtgctgc gtgacctggg cgtcgtcgtc gaccaccccg
    34441 cgccgaacac ctatgaagtg gccgcgggcg atgtggagtg gctcttcgtg ccgctcgagg
    34501 ccgcggccaa aatgcgtgcc tccttcatcc tgctcgggcc gctgctcgcc cgtttcgggc
    34561 gcgtgatcat gagcaacccc ggtggcgacc gcataggccg ccggccggtc aacctgcacg
    34621 tggacgcgat gcgcgcgctg ggcgctgaca tcgagtaccg caacggctac tacttcgcgc
    34681 gctcgaacgg ccggctgcgc ggctccgaga tcgcctttcc ccaggtgacc gtgatgggca
    34741 cggagaacgc catccttggc gcggcgctgg ccgagggccg gacgaccatc aggcccgctg
    34801 cccaggagcc ggagatcgac gacctgatcc tgttcctgca gaagatgggc gcggatatcg
    34861 agcgcaccgc gccggacacc atcgtcgtca acggccagcg ccggttgcgc ggcgcagacc
    34921 accacgtgct gcctgatcgc atcgagaccg gcacgttcct gattgcggcg gcggtcaccg
    34981 gcggcgaggt gaccctagag ggcgcgccgt gcgaccacct gggcgcgttc ctcgacgcga
    35041 tgacggacat cggcgtgccc gtggcatgcg ggcgcaacac gatcgagatc gcgtgctccg
    35101 aggatgcggt ccggtcattc aaggcgcgcg acatcgagac ttcgccctac ccaggcttcg
    35161 cgacagacct ccagtcgccc acgtcagtgc tgctcagcca ggcgaccggc acgagcaggc
    35221 tgcacgagac gatcttcgag gaccgcctcg aatggctggc cgagctgcgg cgcatgggcg
    35281 cgcaggtcga cctgctcgac gcccacaacg cctcgatcaa gggaccggtc aagctacacg
    35341 cggccgatgt cgagatgggc gacctgcgcg cgggagcgtc actgatcctg gccgcgctga
    35401 cggccgaggg cacgagcacg attcacggcg cgcaccacgt tcggcgcggg tatgagaaca
    35461 tcgaaggcaa gttcctcgat ctgggcgcgc agattgagca tgtctccgaa gaggcgcgcg
    35521 agggcgacta gtggatatca gcaactgatg aaaatcggca tcgatctggg cacggccaac
    35581 atcctcgtct acgtgaaggg ccgcggcatt gtggcgcgtg agccttcggt cgtggccgtt
    35641 agcgacgaca accacatcgt cgcggtgggc gaagaggcgc gcgagatgat cggccgcacc
    35701 ccgggcaaca tcagcgccat ccggccgatg aaggacggcg tcatcgccga ctacgtcatc
    35761 accgaggcga tgctgcgcta cttcatccgc aaggccggcc gcgcactgac caagcccgac
    35821 gtgatgatca gcgtgccgtc gggcgtcacc agtgtcgaga agcgtgccgt gcgtgacgcg
    35881 gcgctcaagg ccggcgcgcg cgaggcgtat ctcatcgagg agccactggc cgccgcgatc
    35941 ggcgccaacg tgcccatcag cggcccgtcg ggcaacctcg tcatcgacat cggcggcggc
    36001 acgtcggaga tcgccgtgat ctcgctcggc ggcatcgtgg tcgcgcgctc ggtgcgcgtc
    36061 ggtggcaacc gcttcgacga ggcgatcgcc aactacgtgc gcaagaagta caacgtgatg
    36121 atcggcgacc gcacagccga agaggtcaag atgcagatag gcacggccat gccgctcgag
    36181 cgcgagctgc acatggaggt gcgcgggcgc gacctcatcg ccggtctgcc gcggacggtg
    36241 cccctcacga gctcagaggt tatggaggcg ctcgagcagc cgctccagca gataatcggc
    36301 gcggtgcgcg cggtgctcga ggagacgccg cccgagctgt cgagcgacat catcgacaag
    36361 ggcatggtca tgaccggcgg cggctcgctg ctgcgcaaca tcgacaagct gctgacccag
    36421 gtcaccggcg tgccctgcca cgtcgcggac aacgcgctca attgcgtcgc cctggggacc
    36481 ggcctggcgc tcgagcactt cgacttcttc cggaagtcgc tcgtccagcg gatctgagcg
    36541 aaccggcaga cctgccggca ccgacgcgca acatggagcc cgattcgaca cgcccggtgc
    36601 gctcgctggt cgacttgcac tgccacacca gcgcatcgtt cgattcactc agcaagccgg
    36661 cagacgtcgc ccgcgtggcg gcggagcgcg gtctgaccca cctggcgatc accgatcacg
    36721 accggatcga cggtgctctc gcggcgcgcg acgcggcgcc ggccgggctc gcgatcatcg
    36781 ttggccagga ggtgcgcacc accaccggcg atctcatcgc gctcttcgtc gagcggccca
    36841 tcccgcccgg cttggcgccg gctgatgcgg cccagcgcat ccgcgagcag ggcggcgcgg
    36901 tgggcctcgc ccatccatat gatcgctttc gtgccggcgc cgcccggccc ggctgggagg
    36961 acgagctgga gcgactgctg ccgcggctcg actacgtcga gacgtggaac tcgcgcttga
    37021 tgctcggcga cggcaaccga cgtgcagccg agctcgccca cgcccatggc ttgcccggcg
    37081 cggccgtttc cgacgcccac acgctgctcg aggtgggcgt gtcgtacacg gtggttgacg
    37141 ggccgctcga tagcgccgcc gacctgcgcg ctggccttgt tggggcgcgc atcgtgacca
    37201 gccacggctc gcgcctggtg cgcctggcca cgccgctggc gaaggtcatc cagcgcatgc
    37261 gcggcaatgg ccgcgtgccg gtggcatgag cgaccccgca gcggaccagc cgtcggctga
    37321 ggcgcgggtc gtcgacgagc cgatcgtcga ctatccgggc gtccacgagg agtcgacacc
    37381 accgcagccg ctgtcgcgcc ggttgcgcga cccgcgcacg atcgtctcgc tggtcctgcc
    37441 catcgtcctg ctcgtgctgg tggcgctcag cctgaaggac ctcgacctca atgaactgac
    37501 gcagacaatc gtgcgcgcga acccatggtt cctgatcgcg gccttcgcca tctactacgc
    37561 cgggttccca ttgcgcggct atcgctggca gctgctgctg cgccgcgccg gcacgcacat
    37621 cagcctgcgc gattcgacgg agatcatgtt catctcatgg ctggtcaact gcctcgtgcc
    37681 ggccaagctg ggcgacgtct atcgcgcgtg gctgctcaag gcgaacttca cggtgtcgtt
    37741 gagccgcacg ttcggcacgg tcttcatcga gcgcatcttc gatctcttcg ccatcgccat
    37801 cctcggcctg ggcgcggcgt tctggagctt ccgcggcaac ctgccgacgg cggtccaggt
    37861 agtgattgcc atcgggctcg cggtggtcct gctgctgagc gtcggtctgt acaccgtgcg
    37921 caatttcggc aagcgcgccc tgctccgtgt gccactgccc aggaagaagc agctgctcga
    37981 gctgtacgag aagttcgagg agggcgtctt cgccatcaac ccgcgccagg tgccgtggct
    38041 ggccgtggtc acggcggcca tctgggcgac cgaggcgatc cggctgctgc tcgtcatcga
    38101 ggcgctcaac ttccgcgacg tccacctggg catcagcggc gcgttctttg tcgcgctgtc
    38161 ggcgtcgctg ctgacggcga tcccgctcac gccggccggc cttgggatcg ttgagctcgg
    38221 catcgtgggc atcctgacgg tcatctacgg cgtggaccag acgaacgcgg tcgcgatcac
    38281 cctcgtcgac cgggcgatca gcgtgttctc gatctggttc acgggtggtc tcatctacgc
    38341 gttatcgcgc aagacgaaag ccaacgtgac ccgacgcccg gcagataggg cgagcgccgc
    38401 cggcggctag cggagccaat gaaaaagccg gtcgtggcga ccggctgatc caggcgagtg
    38461 acgcagacgg accgagcggc cgcgaccgaa cagaatggct agttgcggtt gggcatccgg
    38521 gccgcggcca acacctggcg cggatcggtg gtcacggcgc cgttctggcg cgctgcggcg
    38581 aggcggcgca tccggagggc gaggtagtac tccatcacct ggcgcacctg tggctcgctc
    38641 aagaggtcgt ctgagcgaag cgcgtactcg acgcgcgcag tggggtggct cgcagtggag
    38701 gccacgaggc caaagtactg cggagtctct gtctcgtcgc gcagctcacg gaggccgcga
    38761 gcgagctttg tggcggtccc cagcgacgga gtgcggtcac cccgcacaag gcgcgagatg
    38821 gtcgagtgat ccacacccga ctgctgcgcc agctggcgct gcgacatctt cttcgccttg
    38881 agctgagtcc tcagccagtc gttgaaggcc cgtccgttct ggacgctcat tggatcccct
    38941 gctgcgtgat cgggcgtggg cacagcggca taatccggtg cgcgggtgtt gctcgatata
    39001 ggcccttggt acggtgccaa aacggcctac cgggtcaagc ccccggaggg gccgagcgcg
    39061 ctgctccgcg gcgcaggttg aacgcctccg gcggtagcac cggcagctcc tggccgtcgg
    39121 ccagcatgag cgcccgctcg aggttgatcg cgtcgtagcc ggcatccatg ccgggcgtct
    39181 ccaggaaggt cggcagggcg cccagccagg ggtgattcag gagatcgcgc aggccgtcgc
    39241 ggccgatctt gccggcgccg acatgctcgt gccgatcgag gcgcgacccc agctcgacgc
    39301 gcgagtcgtt gagatgcagc atgacgacgt tggcgcgccc gagcaggcgc tccgcgcgct
    39361 cggtgagcca ctcgatccag gtgctgctgc ccacgtcgta cccaccaccc cacaggtggg
    39421 cggtgtcgag acatacaccc aggcgttcca tgggcagccc ggccgcgtcc gccgcggcca
    39481 ggatgtcagc taggtcctcg agcgacgagc cgatgccgtc gcccgtgccg gcggcgttct
    39541 ccagcaccag gcgcggcaga aggcccggcg ctggctcgct ctgatcggct tcgcgcaaca
    39601 cggccgcgag gccgcggacg aggcgctcga tcgcgctgtc gcgctcgtca ccgcgatgcg
    39661 agccgatgtg catgacgacg ttctgagcgc cgtactcacg gccgacaact agctcgtggg
    39721 ccatggtggc gacggagcgt gcccagaact cctcgttcgc gccgcacagg ttgaccaggt
    39781 agggcgcatg aatcgcgagt tgctcgatgc ccgctgccgc aaggcgctga cggaactcgg
    39841 gcagctcggc cggcagctcg tcacgccgcc gccaggcgac cgggttgtcg gtgaagacct
    39901 ggacggcgtt ggcgccaatc gcctgtgccc ggtcggcggc cttgagcaac ccctggccga
    39961 gctgcagatg gggcccgatg agccgtttga agtcagtcac ggtctcgatg ttttccagca
    40021 aagccgattt gacggccatc atattgtggc ctcatggatc ccgatctcct gaccgccctg
    40081 aaagccctat cggatgcgtc acgcctgcgc atcgtcggcc tgctcgccga cggtcggcgc
    40141 atggccgtcg aacagctcgc cgccgcactc aaggtcacgc cggcgacggt cgtgcatcac
    40201 ctcaggaagc tgcgcgaggc gggactggtc gaatcgcacc cgcggccgcc gtacatggac
    40261 tactcgctcc gactgggccg cctgtccgag atcggcgcca gcctgcaccg catcgcgcgt
    40321 gagcagaccg gcgcggcgga agtggacccg acgctccccg actgggccac gccggccgac
    40381 gccaaggtgc tgcgcgcctt ctttgatggt gagcggctga ccagcatccc ggtccagcat
    40441 tcgaagcgcc tcgtcgtgct gcgccgcctc gccgagacca tcttcgagaa gaagcgcaag
    40501 tacccggaga aggaggtcaa ccagctgctc gccgtgcgtc accctgacgc ggcgtcgctg
    40561 cgccgctacc tggtcgacga gggcttcatg gcgcgcaagc agggcgtgta ccggctccag
    40621 ccgcgcaagg actggcccaa ggtgggttca ggccgctagg cggtgatgcc tgggcgacgg
    40681 gccctaccgc tcgcgcgcga gggccagccc cgacacggca tgcgcgcccg ccgcatacaa
    40741 cgccgcggcg cagccgctga gcgtcgcgcc ggtcgtaacg acgtcgtcga cgagcacgat
    40801 ccagccgccg ctcacctggc gtcggccgcc ggccttgatc gagaaggcgc cgcccacgtt
    40861 cgcggcgcgc gcgcgccggc caaggctgtg ctgcgccgcg gtgcgcgccg cccgttcgag
    40921 cacggtcgcg accggcatct tgagcaagcg gccggcggcg cgcgcgagca gctcggcctg
    40981 gtcaaagccg cgttccttga gccgcgcggc gtgaacgggc accggcacca gcacgtcacc
    41041 accgatcccg gcgcgccccc agcgcgcggc catctgctcg gccaggacat cgaccaagcg
    41101 cagctcgccg tcatacttga gcgcggccag cgcggcgcgc gccacaccgc tgtacgagca
    41161 gcaccattcg agctggacga gcccccgggg ctgggtggtg gcgagaccca gcggcacgcc
    41221 gggcggctcg tcacggcgcc gcgcgaggga gggcaggcac ttactgcaga ccgcggcgcc
    41281 ctcgcggccg cagcccggac aggcgggcgg cagcaggaca tcgagcaacc gtcgcatcgc
    41341 ccctgaagat tggcgcgcca cgctcaccgg cggctcatct ggcgcttggc gcgcgcttat
    41401 catcgcggcg atgctgccgc cgacggccct ggacacggca cgcagatgac caatcgccgc
    41461 tgggcgggac cgctggcgct cgggcttgcc gcgctcgggg tgctcgtcct ggtcggggtg
    41521 ctggccgcgt ggttcaacga gtcgcgcggc tgggcctacg acttccaccc ctattacgac
    41581 ggcgcattgc ggctgctggc caacggcagc ccgtaccaag cggtgaccct cgacggtccg
    41641 ttctcaccgg gacccaaggg cctgtacctg tactcgcccg tgctcgccgc gctgttcgtg
    41701 ccgctgacct ggctgggcga ggggagcgcc atcgtcgtct gggctacgct gcatatcgcg
    41761 gcattggtcg cgctctgcgc gctgatgcca atctcgcgca atctgcggct cgcgaccttc
    41821 ggcatcgcct gtctcagcgc gcccgtcctg tacgacatcg acctgggcaa cgtcagcctg
    41881 ttcgtcacgc tggccgcggt gctggtctgg cgctggctcg accggccggg gagcggcatc
    41941 gtgctcgccc tggcgctgct ggtgcgaccg gccatggcgg tgatctgtgg ctggtggctg
    42001 ctgcgcggcc tgtggcggcc cgtcgcctgg gcagttggca cattcgccgc ggtcgctgcg
    42061 gtcagcctcg tttggctgcg gccggacgtg tggtgggagt ggctgaccgt cctgcgcaac
    42121 gtgagcaacg tgacgggcgt gaaggccaat gtcgacctgg gctcggccgt gctgatggtc
    42181 ggtggctcgg ccgaggtcgc tcagatcgcg ctgtacggcg gctacgtgat cgcggttgcg
    42241 gccatcctgc tcagcctgcg ccgcgaccgc gagctgtcat tcgtggtcac gctcatggcg
    42301 acgctgctgc tctcgccgct gttgtgggac cactacctga ccaacctgct catcccggca
    42361 gcgttcctgg ccgcgcgcgg gcgcgcctgg ggcttggccc tgccgctgct gtgctgggcg
    42421 ccgcagatca tcgccgtgct cataccggag atgcggaccc atgccgacgg agcgctggcg
    42481 ctggtggcgg tcctgggtgt cgtgctgccc ttcctggcgc ccgactcaga tgcgcgcgcc
    42541 ggcttcttct gggagcgtcg ctgggggcct ttacgcggcc ggcgccaggg tgacgtaatc
    42601 gccgacgctg aggcctgagc tggtcagtga accggctggg acctcgacgg cgccgcgcgc
    42661 gccgcgcacc cacggcacga gcccggtcca cggccgcagg ttcggtttga gcgcaaccac
    42721 catccgtgcg ccgtccgcgc gcggcgcgcc aacgaatacc acgtcgatcg cgaagcgcat
    42781 gaagaacatg tgcaccccgt tggtgccggg caggtagagc ccctcgcccg cggccaggcc
    42841 acgccggccg atcaggccgc gtagccgcgc cagccacgag tcggcgacat cgagctgcca
    42901 cagtggtgcc gccgaacccg cggcgtgcag ctccacccta ttgctcggct tcttgtcgct
    42961 caccgcgaac gatgatgcgc gatcgagcta ctggccgttg ctcatgttga gcgcgatcag
    43021 gatgatcgcc gggccaagga tgacgatgaa catcgagggg aagatgcagc cgacgagcgg
    43081 gaagagcatc ttgatgggcg ccttggcggc cagctcctcg gcacgctggc ggcgctcgat
    43141 gcgcagctgc tccgactgga cctgcaggac cttgctgatc gagacgccca gctgctcggc
    43201 ctggacgatc gcgccgatga agttggacag cgcctgcacc tcagttcggc cgacgatgtc
    43261 gcgcagtgcg tcgcggcgcg tcttgccgac acggatctcg gccagtgccc ggcggaactc
    43321 gtcggccagc gggccgtgcg tcttctcgac aaccttggcc agcgcgccat cgaacgacag
    43381 gccggcgcgg accgagatgg tcagcaggtc gagcgtgtcg ggcgtggcca gcaggatggc
    43441 cttctggcgc gccttgatgc gtcggccgag ccagatctcg ggccccagga agccgaaccc
    43501 gccaatggcc agcgcgccga tgaagccgaa gggcgcgttg cccatcagga tgccgaagac
    43561 cgcgaacgcg cccgcgccga acaggccggc catgaccagc ttcatgccga ggaaatcgat
    43621 cgttcgcaga gcgcccgggt tgccggccat cgacaggcgc ctctccgtgc gactgacctt
    43681 ggacggactg gcgaagcgct gagcgatgcc tgacagacga gtagccaggg ggcgcagggt
    43741 gcgctcgaac aacggctgct ggagctcgat ctcctcgagc gtgcgggcct gcatcgagcc
    43801 gagctgcgac agccgggcct ggaccgggtc catctgcttg gatccggcga gggcgaggaa
    43861 gacgagcagg acgcccagcg cggcgacgcc ggccatgatg atcggaagca tcgctagacc
    43921 tcgatgtcga cgatgcgccg gatgaggatg aagcctatga gcatcatgaa tccgcctccg
    43981 gcgagaatga acatgcccgc gggcagcccg agcagctcgg ggggcttctg gagcatgggc
    44041 gccatgaatg ttggcgcgat gaccgatagc agtaggacca gcgcgatcgg caggaagcca
    44101 acgacatagc ccgacatgcg ctgctgcgcg gtgagcgttc gaatctcgcc cttgatgcgc
    44161 acccgctcac ggatggtgaa cgcgatgctg tcgaggatct cggcgaggtt gccgccgacc
    44221 gtgtgatgga tggcaatggc cgtggtcatc aggtcgaggt cgtccgagcg cacgcggcgc
    44281 tggagattgg ccagtgcctc gtcgagcggc aggcccaggt tgacctcgcg gatgacacgc
    44341 gcgaactcgg tcgacatggg cggtcgggtc tcacgcacga tcatttcgac cgcctgcagg
    44401 aaggacgatc cggcgcgcag cgagttggcg agcagcatga tcgtgtcggc gagcccggta
    44461 ttgaacgcct tgagccgctt gcccttgcgc cggccgagcc agaactttgg cagccagtac
    44521 ccgaccagca gcgccgcgat ccacgcgatt gggctggcga acagaggtat gagtgggctc
    44581 aggccgacga acagcagggg cagcccgagg atcgcgccag cgcgaatggc caggaactcg
    44641 gtgggcttca gggccaggtc ggcccgggca agctcgcggg cgaggttgct gccccagtcg
    44701 cggcgctcga cgatgcggtt gaagttggcc agcgcggctg atgtggcgat gcgttcgccc
    44761 aggtcgcgct tggcctctgt gtcgtggcgg tcgctgtcgc ccgcggcgta gcgctcgagg
    44821 cgcgcgttca cgtcggagcc accggcgagg ctgacgaaga tcagcagcac gccgacggcg
    44881 gcaccagcgg cgacgaggat gacgagtgga tccatggcta gatcggctgc ccgaacatgc
    44941 gcggcggcag gtggatgccg tgcgcctcga acttctcact gaagcgcggt cgcacaccgg
    45001 tcgggcgcag ccgaccctgg accttgccct cgacaatgcc ggtctgctcg aacatgaaca
    45061 cgtcctgcag gacgatgacg tcgccttcca tgccctggac ctcggtgatg ttggtgatct
    45121 tgcgcgaccc gtccttgagc cggtcctggt gcacgatcag gtcgacggct gaggcgatct
    45181 gctcgcgcac agcgcgcagc ggcagctcga cgccggcgaa cagcaccatt gtctcgaggc
    45241 gggcgagcat gtcgcgcggc gtgttggcgt gaccggtcga catggagccg tcgtggccgg
    45301 tgttcatggc ctggagcatg tccagcgcct caccgccgcg gcactcgccg acgatgatgc
    45361 gatcgggccg catgcgcagg gcgttgcgca cgagctcgcg gatggggatc gcgcccttgc
    45421 cctcgatgtt ggccgggcgc gactcgagcg tgaccacgtg ctcctggcga agctggagct
    45481 cggccgcgtc ctcgacggtg acgatgcgct cgtcgttggg aatgaacgat gagagaacgt
    45541 tgagcatcgt cgtcttgccg gagcccgtgc cgccgctgac aaagacgttg aggcgcgctt
    45601 cgacgcacgc ccgcaggaag tcaaacatct ccggtgtcga ggtgccgaag cggatgaggt
    45661 cgtcggtggt cagcggcgtg gccgcgaact tgcggatggt caggaccggg ccgacaagcg
    45721 agatgggtgg gatcaccgcg ttgacgcgcg acccgtcgac gaggcgcgcg tcgaccatgg
    45781 gactcgactc atcgactcgg cggccgatgg gcgagatgat gcgatcgatg atgcgcatca
    45841 cgtgctcgtc gttctggaag gtgacgttgg tcagctccag cttgcccgag cgctcgatgt
    45901 acacctggcg cgggccgttg accatcacct cggtgatgcc ctcgtcgcgc agcagcggct
    45961 cgagcgggcc aagaccgacg atctcgtcgg tgatctgctc gagcatgcgc gtgcgctcgg
    46021 cacgggtgag cgccaggccc tcggcgtcga gcgcgcggcc gaatgcttcc tcgatctcgc
    46081 gccggacctc gaccgcgttg gccaggtcga gcttgggatc gatgtcactg atgacgcgct
    46141 gctgaatgcg gaacttggca tcgcggaacg actcgcgcag cggcacctgg gcgagcatcc
    46201 gcccggtcgt cggggcaccg ccctggccgg gcgagccagg agccggggga ggcgcggtcg
    46261 gcagccgctc aaggccggcc ggtggccgga ccggcggctg gctcccgggg ttctgcgacg
    46321 cagccggatc cgccggggcg atgcccggca tgtcggcggc tggtcgtgcg ctttctatgc
    46381 ggcgcagcag agacatctag cccttctcct ttagtgggcg cgccactgga acaggccacc
    46441 tgactttcgc tgcgcggcgc gcgctggggc ctgacccggc tgtacctgct cgccaccaac
    46501 gatggtcttg gtcaagcgca cgacgtcctc ggcgacgcgc gtctggcgcg cgccgacgac
    46561 gaacggcacg ccgcggttga gggcgtacac gacggtccgg ccgtctgaca cgatgctgtg
    46621 gctgatcttg cgcccgatcg agctctcgac gtcgtgcagc cggatgccgt agccagagtc
    46681 ggcgcggttg agcagcagct ggaccttgcc ctccggatag ccgagctgct cgacgagggc
    46741 caggaactgg cggatgttct tgatgttggt tatctcgagc gtcagcacgg tcagcacgat
    46801 gtccgactga tcgaggaacg cgagcgtcac gtcctggagc agcggcgagc agtcgaccac
    46861 gaccaggtcg tgggtcaggc gcagcgcgga gatgatcttt gagacgtggt cgggcgttac
    46921 gagctcggcc atctccgggc tgggcggcgc catcagcacg cgcacgccgg tgctgtgatc
    46981 gacgagcgtt gtctcgagcg tctccatgtc cccgcccgcc atctcgcgcg ccacatcggc
    47041 gatcgtctgg ttcttgggat tgaggttgag cagcacgccc acatcgccga attgcaggcc
    47101 gccgtcgacg agcgccacct tcttgccgag ctcggccttg gcggccacgg cgaagttgac
    47161 ggccagtgtc gtgcgcccga cgccgccctt gggcgagaag aaggtcacca cctggccgtt
    47221 gtgggcctgg atgggctcgg catggccgtt gccggacgcc gcgggtcgga cgctggcaac
    47281 gcgatcgagc ttctggcgct cgcgctgatg gacgtttcgg atcgacgccg cgagctcgtc
    47341 gctgctgaac ggcttgacga ggaactcgcg cgcgccggcg agcatcgcgc ggcgcaggta
    47401 gtcgggctcg ccctggacgc tcatcatgat gatcgaggcc gtgggcacac gcagcgacag
    47461 ctgctcggcc gtggcgatgc cgtccatgcc gggcatgttg atgtcgagca gcagcacgtc
    47521 gaccggcgct tgcgtggcca ggctgagcgc ctcctggcca gaaccgacgc tgccgacgac
    47581 ggccatgtcc gactcgaagc tgagcagctt cgccaggtgg tcgcgcgtct cgatgatgtc
    47641 gtcgacgatc accacccgga tcggctcagt tgtgctcacg gacgtgcggc ctccccaccc
    47701 accggttcag ggcgtgaccg acttgggcgg caggaccccc cattggtcga ccagctcctt
    47761 cagggtgata ccgctggtgg ttgtgtccgg tgccccgcta tcgccgggcg cccgcatgag
    47821 gagcgacacg ttgccgtcga gctgggcgaa acgcacgact tcggattgct ggggcgagac
    47881 cgcgagcacg acgaccacgt ccggctcggg cacgccattt ggcgtaaccc cgttgttggg
    47941 gtcgtttgac cgcgccatga ccgccaggac ctgcacgttc tgcacgacga ctttgacggt
    48001 ggtgttgttc atgaattcgt cgaccgagat gtacgggttg gtatccacca ccgtgccatc
    48061 cgggctcacg tggccgtagt tcgggttggg cagcacctgc gggttgagcg agtcggtgtc
    48121 gcgcatcgag atgaggacgt cgacccgatc accgggctgc agcagcgcgc ccacggcgtc
    48181 caccttgctc agcggaatcg cgatcgcgac gagtccgggc tggatcgacc cggcgatctg
    48241 ggccacggcg gtcgtgtcga aatcgctggc cgtgagggcc tggcctgccg ggacggcgcg
    48301 gcggacgatc tggcccacca gctcctcggg atgctgatat gtgcccgagg catctgcctg
    48361 cgggcgcggc tgagtggtca gcttgtcggc cgtgagctcg gtgcccaggg tcaggtcgac
    48421 tgcggccacg acgacgttga cgtcggccgg cggcgcatcg acggtcgggc tgcccaggcc
    48481 gccgaatgcg agcaccgcca cgaaggcgac ggcggcgagc aggacaccgg caatgagcat
    48541 gaatcggttg gcgcgcttca aggtgcctct ccaagcgtgg tgtgacaggt gtcaacgacc
    48601 atagaagcgg tgcaggggtg tcgcgatgcc ccgaatgggc taggaccatg cgcctctgca
    48661 ccgtaccgac cacccgcctg gtgcccggcg aaacgacccc tcattcacgc tggccagggc
    48721 gctgggcgca caagaaaaga gagcgccacc tggtcccgcg agataggact caggcggcgc
    48781 tcccacgagc gggcgctccc gctgccgcgg gccctccacc cgctacatgc ggttcccgag
    48841 gtcggacagc atcgagttga tcgagccgcc catgaaagtg atcgcgatga tcgacgccac
    48901 cgcaatgagg gccaggatga gtgcgtactc ggccagtccc tgtccggcct gcttcattcg
    48961 agcaggcgcg cgcccagatg cgaacttcac ttcagtgctc cgtcgataag tgcttgcatc
    49021 gcaatagccg cgacgctgcc ccgactctgg cccaggccac tgggggccga aataggaaag
    49081 cggtacccat tggcggcgcc gcctgccgtc gcacggggca gctgcgcaac gaggcggtgc
    49141 gtcaaggctc cgccaccgcg cggacgacgc tagccgttga tcgtgtcggc gatgatcgag
    49201 aggatgtcgt tgatctgggt gccgaacagg gtcagcgcga ccagtgccac gatcacgatg
    49261 agggcgacga tcagcgcgta ctcagccagg ctctgaccgc ggtcgtccat ggcgccgatc
    49321 gtaaggcatc acgccctggc cgattggcgg tgtgccgggc aaaggaaaag ggcgtcggcc
    49381 ttgcggccga cgccctgctt gtcgcttgtg gcgaacgaga tcagatcttg ttgccgacgt
    49441 tcgacaggat gctgttgatc tggccaccga ggaacgtgac cgcggcgatc gccaggacgg
    49501 cgatgagcgc caggatcagc gcgtactcgg ccagaccctg accgcgctcg tcgctgcgaa
    49561 tcaggctcac caggtactga agcattcgaa caccctccgg tattggatta gtccatcaag
    49621 aaggacgcgc caaagatgcg ccgcaccgca gcgctgccga aatgggacga gagtccaagc
    49681 cgggtagcca ctagtcctgc ccggattcgt gctcggtgac cggtcaggcg gggcatcgag
    49741 cacgcccgca gcggggcacc gagccgctcg cagcggggcg tcagcccgtg gctgtgctca
    49801 cctcgagcgt cgaacggtcg ctcgacaggg tcacctgcag ccccgcagca tcagcacgca
    49861 gctggatgtc gctccacgcc ccctcgggca gcccggtggc acgcaacgtg tcgtcgcgct
    49921 cctgttcgcg gatcatcgat gccagggcgg cgggcagagt tcgttcgtgg cgcgcggcgg
    49981 caacgacggc gtgggcccgg gtcgcggcgc tctgggtggc ctgcgcgctg ctggcctgga
    50041 tcgtggcgct gacgctgcct gcggtccagt tgagcttcac gctgagcgcc gcgggcgcgc
    50101 ctgacaggta accgctcatg gcgtcgtcga tgatccggaa cagcgcgctc tcgaggtctg
    50161 acctcaggcg tcggtctgcg ccgaccgatt cgaagcgcac cgcgaggccc gcgcgccggc
    50221 cgcgctccag ggacgagcgg cgcagcgtcg gcacgaggcc caggtcatcg agcaccatcg
    50281 gccgtacgtc gaagatgaaa tccttggtcg cggcgagcgc gtgctcgacc atgcgcacga
    50341 gctcggccag ctcagcctgg gcctgctcgg gctggcgctc gaatagccgc tggaccacgt
    50401 gcgcctgcag ggcgatgtta gcgatgctct gcgccggccc atcgtgcatc tggcgcgcta
    50461 tctcgcggcg cagttgctct tgggcggcca ggtcctcgcc cgggttcgta ttgggggccg
    50521 aggcggccac aatgccgtcc ggggggacgg cgcgcatgac gggcagtatc tctgccagcc
    50581 ggtcgcggaa gcgctgcagc gcgcgttgct tgccgccgag cacgtcgagc tgggcctgca
    50641 tcagcgtcac ccgtcgcgtc tgggccagga gcgccgtccg tgcctctgcc agctccggcg
    50701 caacaggctc gttgcgctcg agcgcggtga cgcgttcctc ggcctggatg cgccgcgcct
    50761 cgtggcgctc gctctcggtc gcggtctggc gcagcagcat ctcgatctcg gcaagctcgc
    50821 gctcgagcgt cgcctggtcc gcggcgagct cgcccgccag ctgaggcagc gtcctatcca
    50881 tcggtcgaat ccacacccgt atccgtgctc aagatcagtc ctcggggatc cggatccagc
    50941 cctgcttcat cgcgtagacc acggcctgcg tgcggtcgtt gaccgacagc ttgcgcagga
    51001 tcgagctcat gtggttcttc accgtctgct cggagatcgt cagcgcatac gcgacctgct
    51061 tgttggtcat gcccttggcg atgttgtcca ggatctcgac ctcacgcggc gagagcggcg
    51121 cgaagatggg agctgcttcc tggccgtaca ccgccagctc gcggaactcc ttcaagacgc
    51181 gtgacgcgac cgacgggcgt gaaaagacct tgtcgttgat caggtactcg ccggcgcgaa
    51241 cgcggcggat gatggtgacg aggtcatcag ggttgatgtc cttgaggatg aatgccgcgg
    51301 caccggcctt gatggcctcg aacagctggt cctcgtcctc tgcgaccgcg agggcgatga
    51361 tgcccgctga cgggacctcg cgcttgatgc gctgcgtcgt ctcgatgccg cccggggcag
    51421 gcagcgacag gtcgagcaag acaatgtcgg gcgtcatctc caccgccatc gagaatgctt
    51481 cacgcgcgtc ctcggcctcg gcgacgatgt cgaaatcggg ctccttgtcg agcatctggc
    51541 gcatgcccat gcggaacagg gcgtggtcgt cgacaagcag gatcttggtg cgctgctcgg
    51601 ggttgcgccg gtcaggccct ccgcgtctcc ggtcaggccc gctgtaatcg gcttcgttca
    51661 ctggtgactc ctctcttgta gagcgatggt gagccgcacg accgtgccgg cggcatggtg
    51721 ggatttgatg gccaggctcg agccgagcaa ttcagcccgc tcgcgcatga aacgcagtcc
    51781 gaagtggcgg cggttgggat tggcagtaac cgtcgtgtag tcgaagccgc ggccgtcgtc
    51841 accgacttcg agcacccacg tcgggctgcc gtcgggagct acgccctcgc tggtgtggat
    51901 ccatgcccga ctggctgatg cgtgtttggc gatattgcgc agggcctcct gggcgacgcg
    51961 cagcacgaca gtgcgcgcgg cggcatgaag atagttgccc gcggcttcga gccggagctc
    52021 gattggcagt ccggagcgct ggctgattgc cgacgcgctg tcgcgcaggg catcgtcgag
    52081 cccgacggcc tcgccgagcg acggccggag ctggttgatg tagccacgca gcgtgtccag
    52141 ctcgcgctcc agcagcgtgc gcagcgcggt cagctccgta accgcggcgg ccggttcttc
    52201 gcgcagcgcg cgctcgacga gctgcgtctg gaagatggcg ttggcaaagg cctgcgccgg
    52261 accatcgtgg agctcctcgg cgaggcgaga gcgctcctgc tcttgggcac ccaggagagc
    52321 catcgcgatt cgcggatcag cctcgagcgg ggtagcgtcg gccgccagtg cggactccgc
    52381 ctccggcacg ggagacgcat cggcttgcac ctggcggttg gcggccaagg tgccgttctc
    52441 catcgaattc ggagtatagg tcgctgggac gtcccgactt gtggtgcgcg cacgcaccac
    52501 gtcgcgctcg ttgtcgctca tcgacaggcc tcattgaccg tgaaggcggc actctcggaa
    52561 ggcattccgg gcacctccac gaagcaagcg gcagcttcag cggagcgcaa cacgcccggg
    52621 aaagcgccgc gcggctcggt cacgacgacc agcccggcgt tgaagtcacg cgccagctgc
    52681 gtgatcgagc cgaccgcctc gtcgggcagg gcgagggccg gccggccggt ggcactcgac
    52741 aaccaaatgg gccgatcggt gatcacgggc tcggcgccgc cgggcaaggc cgccgccacg
    52801 ctctggatgt gagcgctatc ggtcagtgca gcgttggccg cgccgaccag ggcgagggac
    52861 gtgatgggaa tcgtggccag ggcgagcgct gccggggcga gccacgagtt cgagcgcggc
    52921 cagctccgcc agacgcggat gcgcgccacc aaggcgtcta ggcccacgac ggcggccacg
    52981 atcaagccga cgtggagcgg cccggcggcg tgctcgaacg tgccccagag cgtcgcgacc
    53041 gggaagagca cgctcgttgc gaagaacgtg atggcgccgc tcaacagcaa catggcgagc
    53101 ggcccctgcc tggagagcga cgtgcgtccg gcgagagcgg ccgccacggt cacgaggccg
    53161 atgaccgcca ccgggccagc gccaaccacg agcacgtcga ctgcgtcgtg ccacaacgcg
    53221 cctccgatgt tgcccaggat cgtgagcagg ccctgggcca ggaacccgct gagcgtcggc
    53281 tgatcggtgt aggcgaagat ctgctcgttg cgcgtcagga agatgttgtc ggtcacctgg
    53341 ccgggcagcg gcgtgccgaa tacggcaagg ttgcgcgccc accagggcag cgcgacgagt
    53401 gccgcgatcg cagcgacccc cgtcgatgtg gcgaaggtcc gggccaccga ctgaccggcc
    53461 agcaggctgc cgaccacgaa ggcgactccc agccacaccg cttccatccg cgtcaggtag
    53521 gcgagaccga gcgtcacgcc cagcgcgatg acgttcttac gctcgccggc aacggcgcgt
    53581 gggatgagca ggcccgccgc gactccaaga acggcgaagg gaatggtcga gtcaggcgcg
    53641 cccgttgcga gcagcagcgg cgccgagctg gcggtcaaca acgcggcgcc gagttcgacg
    53701 gtcatggaac gccgctcgtc cagcttcagg gcgcgggccg tgtcgcgcgc cacgagccag
    53761 gccatcggcg cgagcgtggc gcccaacagg acgaaaccca tctgggccgc gctgaacgac
    53821 gggccgaaaa gagccaaccc ggcggcggcg acgaaggagg ccatcggctg ccagagttcg
    53881 aacgctggcc ggggcagcgt cagtggaggc gtggcgtagc tccagattgc atcgatcacc
    53941 gggccgcggc cgttgaccag gttgcgcgcc acgtcgacgt agtagctcgt tccttctgag
    54001 agcggatcgc gcacgagcgc gatcacgagc aggccgatgg ccagcccgag aacgtagagc
    54061 gcggcaaagg tcgtggcgct cgcgacaagc ggccgccgcc agacgtgggc atggaccgcg
    54121 cccatggtgg gcgccgtcgc cgtcattgct cgcaccgcgg atcttcaggc gtgaggcaca
    54181 ccgcgtagac ggcgcccgcg ggactcgcga cggtcaccgg gttgatcgtc gccactggcg
    54241 cgctgacgcg cgacggcacg acggcgaccg gcgcggacaa ccaggtcgga tgcaccgtgc
    54301 cgttcagcac cggcaccagg gcgggaacga tcgaaccctg ctcgagcacc agccagcgca
    54361 cgtcataggc gcgcatcgct gattcgatgg tcgccaaatc atcgttcggc gagatgacgc
    54421 ccggatggcc gctcaggtag tgataggcgc cgctgtcgcc ggacatgacg cggtcgctgt
    54481 gcggcgcgac gatcagcgcc gcggccaggc gcgactcgac gtcacggacc gcgcgccact
    54541 cgccgatggt gatcagggac tggccgaccg cgcccaagaa ggcgacggcg accgcgccgt
    54601 atgcgaagcg ccgcgtcgcc gtggccgcat cccaggtggg acggcgctca gcgatccagc
    54661 gcaccgcgcc acccacgccg atgaccacga gcacgaacgt gtgggggagg agcgctactg
    54721 ccgagtggat gaatgtgccg tagggcacgt gcacagcgaa gaccaggccg cttgcggcga
    54781 ataggacgag cccgtagatg agcatcggga cgacgttggt gtcgcgcctt cgtgccgacg
    54841 ccccaatgag cgcaaacggc gtcaagacca cgacgagcgg caggagcgcg aactggccca
    54901 gcgcgctcag cagcccgctc agccgcgaag cgaggaacgg cccccagccg tcggcgagga
    54961 gcgtcgcggg cgttgccggg gcggtgacgc tgaacagctg gccgtagtcg ctgatccaca
    55021 ggatccgtcc gttcgccgcc gatggcgcga tcgaaccgaa gacctcgagc tggcgataca
    55081 gccacggccc gtagaacagc ccgaagagcc cgatgcagcc gatggccgcg gaccagccga
    55141 tcacctgccc gcgcccacgg ccacgcacca gctcgcgtgc gaacgcgagc gcgaacggca
    55201 ctccgagcag cacgccgtcg ttgcgcgcca gcgcggcgag gccaacaacg gcgccaccga
    55261 gcacgaacgc gcggcgatca ccgcgcatac cgcgcgcgca cagccacagc gccagcgcgc
    55321 caagtgtcat gaagagccca aagttgtccg gctggccgaa gaacggcgtc aagccgcccg
    55381 gtaccgcggc cagcagcccc gcggccacgg acaggcgcgt ctccaggccg gcgtcgcggg
    55441 caatgaagta ggtcagtggc gccgccgccg ccccaatgag ccacatcggc agctgggacg
    55501 ccagcgctgt ggtgccaagc acccagatga agggcacctg gacgagcacc gccagcggca
    55561 gccagtggcc attggagggg atgggcaggg tcgggtcggt cggtatctgc ccgccgacgt
    55621 cgacgaaatt ccagatgtag tcgatcgtca ggccatgacc gccagccagc gactgggcga
    55681 cgctgtagta gtagtaggag tcggggtagg cgggacccgc gaaggcagcg ccgaccgcta
    55741 ccctgaccag gaaggcggcc gcaaacagtc ctagagcgat catgactgcg gtgcaccgta
    55801 gcaacggacc tagggggcag ggacccgtac caaaggccta gccggtacgc ctaacgggta
    55861 cgaacgggcc atcgttcgcg ctgcgcgcgc gaggcacatt tgcgctcgtg actgcccttt
    55921 tccgccagcc tgcgcagcgg cgcatcctcg tgcacgccgc cgtggtgatc gggctcgtct
    55981 tcgctgccta catcttcttg gtcagcgcgg cgcagaaggg ctcgcttggc ttcgacatcg
    56041 tcgcgtactg gagcgtcgac ctcgcgcacc cgtaccacgg caatgtgggc gacctggggt
    56101 tcttcgccta cgcgccgccg atcgcgctac tcctcgcacc gttcacggcc ctgccgttcc
    56161 cgatcttcgt gacgggctgg tacgtgctgc tgctgaccac gctcgcctgg ctcggcaggc
    56221 gccagatgct gatcctgctc gcctttccac cggtcgcgct cgatctgtac cacggcaaca
    56281 tccacctgct gctcgccgcc gcgatcgtgc tcggcatgcg ctacccggcc gcgtggtcgt
    56341 tcgtgctgct gaccaaagtc acgccgggca tcgggctgct gtggtttgcc gcgcggcgcg
    56401 agtggcgctc cctcagcgtt gccctcgcgg cgaccgcgct gatcgtcgcc ggcaccctcg
    56461 tgttcctgcc tgcccagtgg ctgggctggc tgcagatgct gaccgacagc gccggcacgg
    56521 cgccaccctg gccggcgctg cccgttccgc tggtgctgcg cctgcccctc gcggcactca
    56581 tcgtcctttg gggcgcccgg cgcgacgcac gctggacggt ccccattgcc gccgcgatca
    56641 gcctgcccgc gctgtggccg ggcggcttcg ccatcctcgc cgcgtgctgg ccgctgcgct
    56701 cggctcattc agttcaggca cgacccaaag ccgcgcccca agcaggatcg atggatgtcc
    56761 agccagccgc ggcctagcgt caccctcgtc ctccccgcgt acaacgagga ggcgcgcatc
    56821 ggctcggcgc tgaacgagct gttcggctac ctgcgcaggg gcggcccacc ccgtgaaggc
    56881 tcgcgcccag cgaccgagct cgggcttacc gacatcctcg tcgtggacga cggctcgacc
    56941 gacggcactg cccagatcgt cgccacgcgg cccgagttcg cccccggctc aacgccccag
    57001 ctgcgcctgc tcaaacgttc tcacgagggc aagggcgcgg ccgtcagggc gggcatgctc
    57061 gaggcaacag gtgacttcgt gtgcttcgcc gacgcggatt tcgcgacccc gcctgaccag
    57121 ctgcccctcc tgaccgacgc gctcaccacc cacgacgtcg cgctcggcag tcgtgtccag
    57181 cctgacggaa ccgaccgccg ccagagccag ccgttccatc ggcgcttgat cgggcggttc
    57241 ttccactggc tcgccgcggt gtgggtcact ggtcccgtgc cggacacgca gtgcggcttc
    57301 aagggcttcc ggcgcgaggt cgcgcacgac ctgttcgggc aacagcgcct aacgagcatc
    57361 gtcttcgacg ccgagctcat ccacctcgcc cgccgccgcg gctacagcat cgccaatgtc
    57421 cctgtccagt ggagtgacaa gcgcggctcg cgaatgcgcg ttcgccccgg cctcgcgttg
    57481 cg
//
LOCUS       DATBRX010000132         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_937979, whole genome shotgun sequence.
ACCESSION   DATBRX010000132 DATBRX010000000
VERSION     DATBRX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937979"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..>1871
                     /locus_tag="VIF08_05105"
     CDS             189..>1871
                     /locus_tag="VIF08_05105"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY8135400.1"
                     /translation="MPGEPADVLIIGAGASGAVVAKRLAEAGFKVVCLEQGDWLDKAD
                     YPGTRPDWELRARKEWATSPNIRELPADYPVDDSDSPVAPLMYNAVGGSTIIFAGAWP
                     RALPSDFRVRSLDGIADDWPISYFDLLPYFYRTDRDFGVSALPGNPGYPPDTEDAPMP
                     PLPIGAAGLKVARAHAKLGWHWWPEMNSINSVPYDGRRPCVQRSTCQSGCNEGAKAST
                     DATHWPKAIALGARLVTGARVSRIETNDAGLATGATWIDREGNEHFEPARVVVLAANA
                     IGTPRLLLLSASSRFPDGLANSSGLVGKRLMMHPFANVAGLFEEPLMSWQGQFGDLIE
                     SLEFYETDEKRGFVRGARWGMAPTGGPINAALPSRAGEQAWGPDHHLHFKAHFGHGAN
                     WGLFGEDLPDETNTVVLSKSLKDSSGIAAPEIHYHMSDNTRRMLDFHIERAKESLLEA
                     GAFKIEVDRLMRYSGWHLLGTARMGDDRETSVVDRWNRAHDVPNLYVVDGSVFVTSTG
                     VNPTSTIVAIALRAAEHMVETRFEQQVPEQAKSMQAASWWDAGAERAAAAGGP"
BASE COUNT          315 a          635 c          647 g          274 t
ORIGIN      
        1 aagcgcaacg gggccaagga gatggagcgc ctcaaggcgg ccgtcgagcg gggctgaaag
       61 cccccctttt tccatcttgc gtcgcatata gagaacgttt actgtcaggc cgcgcggcga
      121 gttagggacg gcggtcaacg agggagggcg ccgtcccgaa accgcatctt gggagggcct
      181 ccgcgagcgt gcctggcgaa ccagcagacg tcctgatcat cggcgccggc gcgtccggcg
      241 cggtagtcgc caagcgcctc gccgaagccg gattcaaggt cgtctgcctc gagcagggcg
      301 actggctgga caaggccgac tacccgggca cgcggcccga ctgggagttg cgcgcgcgca
      361 aggagtgggc gaccagtcca aacatccgcg aactgccggc cgactatccc gtcgacgatt
      421 cggattcacc ggtggcgccg ctcatgtaca acgcggtcgg cggctcgacg atcatctttg
      481 ccggcgcctg gccacgggcg ctgccgtccg acttccgggt gcgctcactg gacgggatag
      541 ccgacgactg gcccatctcg tacttcgacc tgctgcccta cttctatagg accgaccgcg
      601 acttcggcgt gagcgcgctg cccggcaacc cgggctaccc gcccgacacg gaggacgcgc
      661 ccatgccgcc gctgcccatc ggagccgccg gcctgaaggt cgcgcgggcg cacgccaagc
      721 tgggctggca ctggtggccg gagatgaact cgatcaactc ggtcccctac gacggccgcc
      781 ggccgtgcgt ccagcgctcg acctgtcaga gcggctgcaa cgagggggcc aaggcatcga
      841 ccgacgcgac ccactggccc aaggcgatcg ccctgggcgc gcggctcgtg accggggcgc
      901 gggtcagccg gatcgagacc aatgacgctg gcctggcgac gggcgcgacg tggattgacc
      961 gcgaaggcaa cgagcatttc gagccggcgc gcgttgtcgt tctcgccgcg aacgcgatcg
     1021 ggacgccgcg gctgctgttg ctgagcgcga gctcgcgctt cccggatggc ctcgccaact
     1081 cgtcgggcct ggttggcaag cggctgatga tgcatccctt cgccaacgtc gccggcctct
     1141 tcgaagagcc gctgatgtcc tggcagggcc agttcggtga cctgatcgag tcgctcgagt
     1201 tctacgagac cgacgagaag cgcggcttcg tccgcggtgc gcgctggggc atggcgccga
     1261 ccggcgggcc gatcaacgcc gcgctaccca gtcgagccgg tgagcaggcg tggggccccg
     1321 atcaccacct ccacttcaag gcccactttg ggcacggtgc gaactggggc ctcttcggcg
     1381 aggacctgcc cgacgagacc aacacggtcg tgctgtccaa gtcgctcaaa gactcgtcgg
     1441 gcatcgcagc gccggagatc cactaccaca tgtccgacaa cacgcggcgc atgctcgact
     1501 tccacatcga gcgcgcgaag gaatcgctgc tcgaggcggg tgcgttcaag atcgaggtcg
     1561 atcggctgat gcgctacagc ggctggcacc ttctgggcac cgcgcgcatg ggcgacgacc
     1621 gcgagacgag cgtcgtcgac cgctggaacc gcgcacacga cgtgcccaac ctgtacgtcg
     1681 tcgacgggtc ggtcttcgtg acatcgaccg gcgtcaatcc cacgtcgacg atcgtcgcga
     1741 tcgccctgcg cgcggcggaa catatggtcg agacgcgctt cgaacagcag gtgccggagc
     1801 aagcgaagag catgcaggcc gccagttggt gggatgcagg cgcggagcgc gcagcagcgg
     1861 ccggcgggcc a
//
LOCUS       DATBRX010000133         3489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2054664, whole genome shotgun sequence.
ACCESSION   DATBRX010000133 DATBRX010000000
VERSION     DATBRX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054664"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VIF08_05110"
     CDS             <1..283
                     /locus_tag="VIF08_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135401.1"
                     /translation="ALNLGGFAGAPTAANADTAAGAPAATAAPASTKTVIDKVYVEAA
                     PKPDVVHVNKPPRNVAGANSARPRGDGRERDGDERERGESGERGEHESD"
     gene            406..750
                     /locus_tag="VIF08_05115"
     CDS             406..750
                     /locus_tag="VIF08_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135402.1"
                     /translation="MRFVYGAGAVAAMSVMAVGLVQPDFAATADQSGSGNAGDTSTVA
                     QVPPDVTVNHVTQYIHLKPGQKAPPGATVIAAAAPTPRVVVTHTSPGGPVAQPAPRPA
                     NPKPVTRQSGRP"
     gene            754..1701
                     /locus_tag="VIF08_05120"
     CDS             754..1701
                     /locus_tag="VIF08_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY8135403.1"
                     /translation="MTAAIHEIVAHSAPSMGGTLSVFISTLPDEAAAAEVAARRTAQR
                     VETWAARLTRFNDTSHLSRLNSAAEASVSIRPTLAAALNWAKLAERRSEGIVDSTLLD
                     ARLAAESGQEMTPDPSPDARSWRVLAGGRTSTVRRDPGIRFDLDGTAKGWIADRAADL
                     LVRWPGAAVDADGDISIRADRGVEWLIDVADPRLGVDGHGPPPLATLKLRGGTSWAAS
                     YGVATSGTTVHRWQLADGRPAHHLIDRRTRRPAHTDVVQGTVVAPTAREAEMIAKSAV
                     ILGSREALGFLARSAALAAILLRDDGSVVSLPGIEAWLA"
     gene            1698..2411
                     /locus_tag="VIF08_05125"
     CDS             1698..2411
                     /locus_tag="VIF08_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135404.1"
                     /translation="MKYQGLNLRTRLLLWGVFGLAIALILYATAPQAVANAADWVTAH
                     QDSLPWYATRILGFLAYGALAVSVLYGLLLSTGILDRIAHRVVSFTLHQDLSAIAIGL
                     TALHGAVLALDTFVPQSVRQLIIPFAGPYRPEWVGIGQVTFYLMIVVYASFSVRRRIG
                     QRAWRLLHYTTLLAFAGATVHGLMAGTDSPAVWAFAAYLVATVAVVFLLIYRITLGLA
                     SSLRPAERVNAPVREELST"
     gene            2408..2707
                     /locus_tag="VIF08_05130"
     CDS             2408..2707
                     /locus_tag="VIF08_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY8135405.1"
                     /translation="MTYVIAEPCVDHMDQSCVAVCPVDCISSVDGVDRKFHIDPDGCI
                     ECGSCLSVCPNNAIYPLAELPAKWADYAWIDAAWFRDAGAARDVLGEVLAQASAA"
     gene            2704..>3489
                     /locus_tag="VIF08_05135"
     CDS             2704..>3489
                     /locus_tag="VIF08_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8135406.1"
                     /translation="MTVLRALPARGAAPVLSARPLATLNATLASREDVTATLAVFVVV
                     LDAPLGAYRPGQYVSLGVERGGELIQRPYSIVSLGMSGTRAELLIRRLPDGRLSNLFW
                     ALPIGARVRVGSARGLFVLDPDDPRPRLLVGTGTGLAPLLAMLADSAARRDAALNILI
                     HGVSYWAELAYDERIAGWAAAGLPIDYRPTVSRPHERRNAGWGGPVGRAEIQLARLLS
                     ESAAARGGVAYLCGNPDMIESCRRVLLAAGVAGADIRVEQFHAP"
BASE COUNT          465 a         1293 c         1186 g          545 t
ORIGIN      
        1 cgcgctcaac ctcggcgggt ttgccggtgc gcccacggcg gccaacgccg acacggctgc
       61 gggcgccccg gctgcaacag ccgcgcccgc gtccacgaag acggtcattg acaaggtcta
      121 cgtcgaagcg gcgcccaagc cagacgtggt tcacgtcaac aagccgccgc gcaacgtcgc
      181 gggcgctaat tcggcgcggc cgcgcggcga cgggcgtgag cgggacggcg acgagcgcga
      241 gcgcggcgag agcggcgaac gcggcgagca cgagagcgac tgatggcagt caaccggccg
      301 ctcccacctc gcccggcagc tcggcctgcg cccggcgcgg gccccgatct tgagaagcga
      361 tccgcgcgcc cggactcccg gcagtcccgg ccagatgcgc ggccgatgcg cttcgtctac
      421 ggcgccgggg cggtggcggc gatgagtgtc atggctgtcg gactcgtcca gccagacttc
      481 gccgccaccg ccgaccaatc tggatctggt aacgcgggcg ataccagcac ggtggcccag
      541 gtcccgcccg acgtgacggt caaccacgtg acgcagtaca tccacctcaa gcccggtcag
      601 aaagcgccgc ccggagcgac cgtgattgcc gccgccgccc caactccgcg cgtcgtagtc
      661 acccacacct cacctggcgg gccggtggct cagcccgcgc cgcgcccggc gaatcccaag
      721 cccgtgacgc gtcagtcagg caggccgtag accatgaccg cggcgatcca cgaaatcgtg
      781 gcccattccg caccttcgat gggcggcacg ctcagcgtgt tcatctcgac gctgccggat
      841 gaggccgcgg ccgctgaggt cgccgcgcga cgaacggcgc agcgggtcga aacctgggcc
      901 gcacgcctga cgcgcttcaa tgacacatcc catctttccc gcctgaactc ggccgcggag
      961 gcgagcgtct cgattcgacc gaccctcgcc gctgcgctga actgggccaa gttggccgag
     1021 cggcgctcgg aaggtatcgt cgactcgacc ctcctcgacg cgcgcctcgc ggccgaatca
     1081 ggtcaagaaa tgacgccgga tccttcgccc gacgcacggt cgtggcgcgt cctggccggc
     1141 ggccggacct cgaccgtgcg ccgtgatccc ggcatccggt tcgacctcga cggcacggcc
     1201 aagggctgga tcgccgaccg cgcagcggat ctcctggtgc gctggcccgg ggcggccgtc
     1261 gacgccgatg gcgatatcag catccgtgcc gaccgcggcg ttgagtggct gatcgatgtc
     1321 gccgacccgc gcctcggggt ggatggtcac gggccgccgc cgctcgccac gctcaagctg
     1381 cgcggcggca cttcgtgggc cgcgagctac ggcgtcgcga cgtcgggcac caccgtccat
     1441 cgctggcagc tcgccgacgg tcggcccgcc caccatctga tcgatcggcg cactcgccgg
     1501 cccgcgcata ccgacgtggt gcagggcacc gtcgtcgcac ccaccgcgcg cgaggcggag
     1561 atgatcgcca agtcggccgt catcctgggc tcgcgggaag cgctgggctt cctggcccga
     1621 tcggccgccc tcgctgcaat cctgctccgc gacgacggca gcgtcgtcag cctgcccggc
     1681 atcgaggcgt ggctggcatg aagtaccagg gcctaaacct gcgcacccga ctgctgctct
     1741 ggggcgtgtt cggcctggcc atcgcgctga tcctgtacgc caccgcgccc caggccgtgg
     1801 ccaacgccgc ggactgggtc acggcccacc aggacagcct gccgtggtac gcgacgcgca
     1861 tcctgggctt cctggcctac ggcgcactgg ccgtctcggt cctgtatggc ctgctgctgt
     1921 cgaccggcat cctggaccgg attgcccatc gggtcgtgtc gttcacgctc caccaggacc
     1981 tgtcggccat cgccatcggc ctgacggcgc tgcacggcgc ggtcctggcg ctcgacacct
     2041 tcgtacccca gtcagttcgc cagctgatca tcccgttcgc cggcccgtac cggcctgagt
     2101 gggtgggcat cggccaggtc accttctacc tgatgatcgt ggtctacgcc tcgttcagcg
     2161 tgcgccggcg gatcggccag cgcgcctggc gactgctcca ctacacgacc ctgctcgcct
     2221 ttgccggggc gaccgtccat ggcctgatgg cgggcaccga ttcacccgct gtttgggctt
     2281 ttgcggccta cctggtggcc acggtggccg tggtcttcct gctgatctat cggatcaccc
     2341 tgggcctcgc cagctcgctc cgtcccgcgg agcgggtcaa cgcgcccgtt cgcgaggagc
     2401 tctcaacatg acctacgtca tcgccgaacc gtgcgtcgac cacatggacc agtcgtgcgt
     2461 cgccgtctgc ccggtcgact gcatctcatc ggtcgacggt gtcgatcgga agttccacat
     2521 cgacccggat ggctgcatcg agtgcggatc gtgcctgtcg gtctgtccca acaacgcgat
     2581 ctatccgctg gccgagctgc cggcgaagtg ggccgactac gcctggatcg acgcggcctg
     2641 gttccgcgac gcgggcgcgg cgcgcgacgt gctcggcgag gtcctcgccc aggcgtccgc
     2701 ggcatgaccg tgctgcgcgc gctgccggca cgcggcgcgg cgcccgtctt gtccgctcgg
     2761 ccgttggcca cgctcaacgc gacgctcgcg tcgcgggagg acgtcaccgc gacgctggct
     2821 gtgttcgtgg tcgtgctgga cgctccccta ggtgcgtatc ggccgggcca gtacgtgtcg
     2881 ctcggcgtcg agcgcggtgg cgagctcatc cagcggccgt actcgatcgt gtcgctcggc
     2941 atgagcggga cgcgcgccga gctgctcatc cgccgcctgc ccgacggacg tctctcgaac
     3001 ctgttttggg ccctgcccat tggagcgcgg gtgcgcgtcg ggtcagcgcg cggcctgttc
     3061 gtgcttgacc ctgacgaccc gcgcccgcgc ctgctggttg gcaccggcac gggcctggcg
     3121 cccttgctgg ccatgctcgc cgatagcgcc gcacggcgcg atgcagcgct caacatcctg
     3181 attcacggcg tttcttactg ggcagagctg gcgtacgacg agcgcatcgc tggctgggca
     3241 gcggccggat tgccgatcga ctaccggccg accgtgtcgc ggccgcacga gcggcgcaac
     3301 gcgggttggg gcggacccgt gggccgcgcc gagatccagc tcgcgcggct gctctcggag
     3361 tccgcggccg ctcgtggggg cgtcgcctac ctgtgcggta acccggacat gatcgagtcc
     3421 tgccgccgcg tcctgctggc ggcgggcgtc gccggcgccg acatccgcgt cgagcagttc
     3481 cacgccccg
//
LOCUS       DATBRX010000134         6642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2502574, whole genome shotgun sequence.
ACCESSION   DATBRX010000134 DATBRX010000000
VERSION     DATBRX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502574"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VIF08_05140"
     CDS             <1..315
                     /locus_tag="VIF08_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135407.1"
                     /translation="RFGELVADEANLHDAATDLGHFAQRVLEVGGANIGVALFARQTK
                     DTHVRIAIATDHGVEEMTRVAFLAGDEGRRRAALACAAALWQFLGRPHLSPGDRSSTT
                     VN"
     gene            424..1089
                     /locus_tag="VIF08_05145"
     CDS             424..1089
                     /locus_tag="VIF08_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135408.1"
                     /translation="MALRLAIALTVLVLANGCSATPTVTEPPAPTATTAVATPTTAAA
                     TSLPPATPTQTEATASPVAVDPCAAALTLADVGPGFTGEPPAAFGGAGLIGFVAGTQV
                     VFQQASGSIGSTVACFDSAANAAATYATWGSSCIDANLDSIPAQTIQIGAIGGDASKG
                     VFCPGDESGSTTISSSTSVYVLLDDVVFSVSVNREEPTGPDGAAVATAISLATALLEA
                     IIG"
     gene            complement(1101..2171)
                     /locus_tag="VIF08_05150"
     CDS             complement(1101..2171)
                     /locus_tag="VIF08_05150"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY8135409.1"
                     /translation="MALSEAVVMDALRTVQEPELGRDIVTLNMVKDLEVTDSQVSMKI
                     ELTTPACPLKDEIERNIHTVLGDIGATEVTVTWGAMVRRAQPAQAQQLLPGVKNIIAV
                     ASGKGGVGKSTVSANLAVALAMEGASVGLLDADITGPNIPMMLGVEGAPAASAEGKIT
                     PLERYGVKVISIQFFVPEGQPIVWRGPLVGGAIQQFLKDVDWGELDYLVIDLPPGTSD
                     AQLTLAQAVPISGALLVTTPQDVALADVTKALAMFRRMSVPVMGIVENMSNFICPHCG
                     EATEIFGRGGGQRFAQRNELEYFGGVPIDVKVRQGGDAGVPAVAQREPGAAAEVLRAL
                     ARQVAARMSVRAAQAAPAITIS"
     gene            complement(2171..3589)
                     /locus_tag="VIF08_05155"
     CDS             complement(2171..3589)
                     /locus_tag="VIF08_05155"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY8135410.1"
                     /translation="MLYPTPAGSVSELMSALVGSAHVTDGRLVIDDEARMRSDGIRAV
                     AWSATFSEEKDVIEAARWIVWEASQLLDAPSASIHDLYMARGRGEVHGFTVPAVNLRT
                     QVFDMAATMCRAANELDVGTIVFELARSEQEYTFQRPGEYVTSVLAGCIYAGWRGPVF
                     VQGDHYQFNAAKYKADPEATAEGIRKLTREALAVGYGNIDIDSSTLVDLSLPSVDEQQ
                     RTNYLRAAEISALIRENEPAGMTVSIGGEIGEVGKENSTEEELRAYLDGYRAELNRRA
                     GDAAAGISKVSVQTGTSHGGVPLPGGGVATVKLNFGTLERLSAVARSYGLAGAVQHGA
                     STLPDELFHEFPRVETAEIHLATGFQNALYEHPAFPRDLLAEIETWCVANCADERKPG
                     EAADVFIYKTRKKALGPYKRQLWELPTKTEILADQQRKLRYLYEQLGVAGSKEMVARY
                     IKPPRVSRPAPVSLMPAAEVAR"
     gene            3649..4602
                     /locus_tag="VIF08_05160"
     CDS             3649..4602
                     /locus_tag="VIF08_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEY8135411.1"
                     /translation="MRTVALQRLPLVILLALALARCAVAPAPSGSPGPTPSPSPSPSP
                     TPATGLSQAELRYALLAEFSPISWCDPDFYPIARQDEQISADEHWPEIVADQPTYRAI
                     TEHLGIAGADPAVLDAAERLAVYRDWKLLSALHLEPLHGGGHAFDLITETNAGLGQGI
                     RFTGTIDAHGVIDIALQEDSFLTACPICLARGTLIDTPAGPVAVQDLRAGDLVWTLDG
                     AGRRVAQPLVRIGTTPVPANHEVVHLVLADGREASVSPGHPTADGRTAGTLRVGDILD
                     GSAITSADRVAYSGGATFDILPDGQSGAYWANGILLGSTLR"
     gene            4625..5707
                     /locus_tag="VIF08_05165"
     CDS             4625..5707
                     /locus_tag="VIF08_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135412.1"
                     /translation="MTKRVEVLVGTKKGAFILESDPARRDWRLRGPFCQNWPTHDFIR
                     AADGTLYAGGGNNWYGPSVWRSTDNGETWTQSSAGMSYGDGEDVPKITTIWKLADAAS
                     NGTIYAGVEPAGLFRSDDHAETWSHVAGLREHPSRPEWTPGNGGLCLHSIVTHPTDPN
                     RMWVAASAVGTFETADGGKTWETRNKGVRADFYPGPAPEFGQCVHKLRLHPDQPEWLY
                     QQNHCGVYRSDDGGRQWTEVTPGLPSEFGFPMVIHPHDPRTIYVIPHSGPADGRHMID
                     GHAAVWRSRDRGDSWQRLDGGLPQSNAYVTVLREAMAIDSLDQPGVYFGTSTGQVFGS
                     ADEGDTWTLMADYLPPIWSVEAAVVD"
     gene            5711..5980
                     /locus_tag="VIF08_05170"
     CDS             5711..5980
                     /locus_tag="VIF08_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY8135413.1"
                     /translation="MATVHLPRSLVTLFTDPPPRHLQLPSTSLAELVDDLDQRWPGMR
                     DRLVEAGPRLREHINVFVDGERQRELTTPLRDGSVVHVIPAVAGG"
     gene            complement(5977..6522)
                     /locus_tag="VIF08_05175"
     CDS             complement(5977..6522)
                     /locus_tag="VIF08_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135414.1"
                     /translation="MTSDQPSLRELTALGELDPGVAALLWLLAEHGLPLTVASPDRSA
                     AERTRAAIVAAAGTGRAARLAPAGGVILADSLEGVLRLLGGDAALNDATRDLGLVVIH
                     DGRRVLSAHYVRPVERDGAGHLQRRPPAVLADWNERAGRFDDFSWAVTDELADRTGLT
                     RQELEDQRDSRRDQLSGSARA"
BASE COUNT         1040 a         2328 c         2205 g         1069 t
ORIGIN      
        1 agattcggcg agctcgtggc cgacgaggcg aacctccacg acgctgcgac cgacctcggc
       61 cacttcgccc agcgagtgct cgaggtgggt ggcgccaaca ttggtgtggc gctcttcgcg
      121 cgccaaacga aagacaccca cgtccgcatc gcgatcgcga cagaccacgg cgtcgaggag
      181 atgacgcgtg tcgcgttcct ggcgggcgac gagggccgcc gccgcgccgc gctcgcctgc
      241 gccgcggccc tctggcaatt cctgggccgg ccgcatctat cccctggtga ccgcagctcg
      301 acgacggtga actagctccc cgatcacgac aagcgcgagc agtcggctgg ttgcgctcgc
      361 cagtcccctt cctcatggca tgggtaccat gccccagctg cgaacaactc atggaggctc
      421 gagatggcgt tgagacttgc gatcgcgctg acggttctcg ttctggccaa cggttgctcc
      481 gccacgccca ccgtgacgga gccgcctgcg ccaacggcca ccactgcggt ggccacaccc
      541 accaccgccg cggccacatc tctccctccg gcgactccga cccagacgga ggcaacggcc
      601 tcgcccgtcg cggtggatcc atgcgcggcg gccctgacgc ttgccgacgt agggccaggc
      661 tttacgggcg aaccgccagc ggcgttcggt ggtgcggggc tgatcggttt cgtcgccggg
      721 acgcaggttg tcttccagca ggcgagcggc tcgatcggca gcacggtcgc gtgttttgac
      781 agcgcggcaa acgcggccgc tacctatgcc acgtggggaa gctcgtgcat tgacgcgaac
      841 ctggactcaa tcccggcaca gaccatccaa atcggcgcga tcggcggtga cgccagtaag
      901 ggagtcttct gtccgggcga cgaaagcgga tcgacaacga tctcgtcgtc gacgtctgtc
      961 tacgttctac tcgacgacgt cgtcttttct gtctccgtca accgtgagga gccgacgggg
     1021 ccggacgggg cagccgtcgc cacggcgatc agcttggcca ctgccctgct ggaggcgatc
     1081 atcggctagg ggcgaatgcc tcagctgatg gtgatcgccg gggcggcctg cgcggcgcgg
     1141 acgctcatgc gcgccgcgac ctgacgggcg agggcgcgca gcacttccgc ggccgcgccc
     1201 ggctcgcgct gggccaccgc gggcacgccc gcatcgccgc cctggcgcac cttgacgtcg
     1261 atcggcacgc cgccgaagta ctccaattcg ttgcgctgcg cgaaacgctg cccgccgccg
     1321 cggccgaaga tctccgtcgc ctcgccgcag tgcgggcaga tgaagttgct catgttctcg
     1381 acgatgccca tgaccggcac gctcatgcgc cggaacatcg ccagagcctt ggtgacgtcg
     1441 gcgagggcga cgtcctgggg cgtggtcacc agcagcgcgc ctgagatcgg gaccgcctgg
     1501 gccagggtca gctgcgcatc ggacgtgccc ggcggcaggt cgatcacgag gtagtcgagc
     1561 tcgccccaat cgacgtcctt gaggaactgc tggatcgcgc caccgaccaa cggcccgcgc
     1621 cacacgatgg gctggccctc cggcacgaag aattggatgg agatcacctt cacgccgtaa
     1681 cgctcgagcg gcgtgatctt gccttcagcg ctggcggccg gggcgccctc cacgccgagc
     1741 atcatcggga tgttgggacc ggtgatgtcg gcatcgagca ggccgacgct cgcgccctcc
     1801 atcgccagcg cgacggccag gttggccgac acggtcgact tgcccacgcc gcccttgccg
     1861 gatgcgacgg cgatgatgtt cttcaccccg ggcagcagct gctgcgcctg cgccggctgg
     1921 gcgcggcgca ccatcgcgcc ccacgtcacc gtcacttcgg ttgccccgat gtcgcccagg
     1981 accgtgtgga tgttgcgctc gatctcgtcc ttcagggggc aggcaggcgt ggtcagctcg
     2041 atcttcatgc tgacctgtga atcggtgacc tcgagatcct tgaccatgtt gagggtcacg
     2101 atgtcgcggc ccagctccgg ctcctgcacg gtgcgcagag catccataac cacagcttcc
     2161 gagagcgcca tcagcgggcg acctcagcgg caggcatcag cgaaacaggc gccgggcgcg
     2221 agacgcgcgg cggcttgatg tagcgcgcca ccatctcctt ggagcccgcg acgcccagct
     2281 gctcatagag gtatcgcagc ttgcgctgct ggtcggccag gatctctgtc ttggtcggca
     2341 gctcccacag ctggcgcttg tatgggccga gggccttctt gcgtgtcttg tagatgaaaa
     2401 cgtcggccgc ttcgcccggc ttgcgctcgt cggcgcagtt ggcgacgcac catgtctcga
     2461 tctccgcgag caggtcgcgc gggaacgcgg ggtgctcgta cagcgcgttc tggaagccgg
     2521 tcgcgagatg gatctccgct gtttcgacgc gcgggaattc gtggaacagc tcgtcgggca
     2581 gcgtggaagc gccgtgctgg acggcgccgg cgaggccgta cgagcgcgcc accgcggaca
     2641 ggcgctccaa cgtgccgaag ttgagcttga cggtcgcgac cccgccaccg ggcagcggca
     2701 caccaccgtg cgacgtgccg gtctggacgc tgaccttgga gatgcctgcg gcagcgtcgc
     2761 ccgcgcgccg gttgagctcc gcgcggtagc catcgagata ggcgcgcagc tcttcctcgg
     2821 tcgagttctc cttgcccacc tcgccgatct cgccgccgat gctgacggtc atccctgcgg
     2881 gctcgttctc acggatcagc gcgctgatct cagccgcgcg caggtagtta gtgcgctgct
     2941 gctcatcgac gctcggcagt gacaggtcga cgagcgttga cgagtcgatg tcgatgttgc
     3001 cgtagccgac cgccagcgcc tcgcgggtca gcttgcggat gccttcagcc gtggcctcgg
     3061 gatcggcctt gtacttcgcg gcgttgaact ggtagtggtc gccctggacg aacaccgggc
     3121 cgcgccatcc ggcatagatg cagcctgcaa ggacgctcgt gacgtactcg ccgggccgct
     3181 ggaaggtgta ctcctgctcg ctccgcgcca gctcgaaaac gatcgtgccc acatcgagct
     3241 cgttcgcggc gcgacacatc gtcgcggcca tgtcgaagac ctgcgtgcgc aggttgaccg
     3301 ccggcacggt gaagccgtgc acctcgccgc ggccgcgcgc catgtacagg tcgtggatgc
     3361 tcgctgacgg cgcgtcaagc agctgtgacg cttcccagac gatccagcgc gcggcctcga
     3421 tgacgtcctt ttcctcgctg aacgtcgccg accaggcaac ggcgcggatg ccgtcgctgc
     3481 gcatgcgcgc ctcgtcatcg atgaccagtc gaccgtccgt gacgtgggcg ctgcccacga
     3541 gcgccgacat gagctcgctg accgatccag cgggcgtggg gtagagcatt acgcgactcc
     3601 tttgtactgt cgtgatcgta aggggaaccg ggatttgatg ggcgcgtcat gaggactgtg
     3661 gctctgcagc gactgccgtt ggtcatcctg ctggcgttgg cgctcgcccg ttgcgcggtg
     3721 gcacccgcac cgtctggttc gccggggcca acccccagcc cctcgcccag cccttctcca
     3781 acaccggcta ccggcctctc gcaggcggaa ctgcgctacg ccctgctggc cgagttctca
     3841 ccgatctcct ggtgcgatcc cgacttctac ccgatcgccc gccaggacga acagatctca
     3901 gccgatgagc actggccgga aatagtcgcc gaccaaccta cctaccgcgc gatcacggag
     3961 cacctgggca tcgccggcgc cgacccggcc gtcctggacg cggctgagcg cctcgcggtc
     4021 taccgcgact ggaagctgct cagcgccctc cacctggaac cgctccacgg cggtggccat
     4081 gccttcgacc tgatcactga gaccaacgcc ggcctgggcc agggcatccg attcacgggc
     4141 accatcgacg cgcacggcgt catcgatatc gcgctccagg aagactcgtt cctgactgct
     4201 tgcccgatct gccttgcccg cggcacgctg atcgatacgc cggccgggcc cgtggcggtg
     4261 caggacctgc gcgctggcga tctggtctgg acgctagacg gtgcgggccg ccgtgtagcc
     4321 cagccgctgg tcaggatcgg cacgacgccc gtgccggcca accacgaggt cgtgcacctg
     4381 gtcctcgccg acggccgcga ggcatcggtg tcacccggcc atccgactgc cgacggtcgg
     4441 acggcgggca cgctgcgagt tggcgacatc cttgacggat ccgcgatcac cagcgcggat
     4501 cgcgtggcct attcaggcgg ggcgacgttc gacatcctgc ccgacgggca gagcggcgct
     4561 tactgggcga acgggatcct cctcggcagc accctgcgct aggggtttcg ctaaagtggc
     4621 gcgcgtgacc aagcgcgtcg aagtccttgt cggcaccaag aaaggtgcct tcatcctcga
     4681 gagcgatccc gcgcggcgcg attggcggct gcgtggtccc ttctgccaga actggccgac
     4741 gcatgatttc atccgcgccg cggacgggac gctgtatgcc gggggcggca acaactggta
     4801 cggcccgtcg gtctggcgca gcactgacaa cggcgagaca tggacgcagt cgagcgccgg
     4861 catgagctat ggcgatggcg aggatgtgcc caagatcacg acgatctgga agctcgccga
     4921 tgccgcatcg aacggcacga tctacgccgg cgtcgagccc gccgggcttt tccgcagcga
     4981 cgaccacgcc gagacgtgga gccacgtcgc cggcctgcgc gaacacccgt cacgacccga
     5041 gtggacaccg ggtaacggcg gcctgtgcct gcattcgatc gtcacccatc ccacggatcc
     5101 caaccgcatg tgggtcgccg catcggcggt gggcacgttc gagaccgccg acggcggcaa
     5161 gacctgggag acgcgcaaca agggcgtccg cgccgacttc tatcccggcc cggcgccgga
     5221 gttcggccag tgcgtccata agctgcgcct gcatcccgac caacccgaat ggctgtacca
     5281 gcagaaccac tgcggcgtgt accgctccga tgacggcggc cgccaatgga cagaggtgac
     5341 gcccggcctg ccgtcggagt tcggcttccc gatggtcatc catccccacg atccgcggac
     5401 gatctacgtc atcccccaca gcggcccggc cgacgggcga cacatgatcg atggccacgc
     5461 cgcggtgtgg cgcagccgcg accgcggcga ctcgtggcag cgcctcgatg gcggattgcc
     5521 gcagagcaat gcctacgtca ccgtcctgcg cgaggcgatg gcaatcgact cactcgacca
     5581 gccaggtgtc tacttcggca ccagcacggg ccaggtcttc ggcagcgccg atgagggcga
     5641 tacctggaca ttgatggccg actacctgcc tccgatctgg tcggtcgagg ctgccgtagt
     5701 cgactgatcg atggcgacgg tccacctgcc gcgctcgctg gtgacgctgt tcaccgatcc
     5761 gccgccgcgg catctgcagc tgccgtcgac ttcactcgcc gaactcgtgg acgacctcga
     5821 tcagcgctgg ccgggcatgc gcgaccgcct cgtggaggcc gggccacgtc tgcgtgagca
     5881 catcaacgtc ttcgtcgacg gggagcgcca gcgcgagctc actaccccgc tgcgcgatgg
     5941 gtcagtcgtg cacgttattc cggcggtcgc cgggggttag gcgcgggcgc tgcccgaaag
     6001 ctggtcgcgg cgcgaatcgc gttggtcctc cagctcctgg cgcgtcagac cggtgcgatc
     6061 agccagctca tcggtcaccg cccacgagaa atcgtcgaag cggccggcgc gctcgttcca
     6121 gtcggcaagc accgccggcg ggcgacgctg caggtgaccc gcgccatctc gctcgaccgg
     6181 ccgaacgtaa tgcgcgctga gcacgcgccg gccgtcgtgg atcaccacca ggccaagatc
     6241 gcgcgtcgcg tcgttaagcg cggcgtcgcc accgagcagg cgcagcaccc cttcgagcga
     6301 atcggccaga atcaccccgc cggccggcgc gagacgagcc gcgcgcccgg tgccggccgc
     6361 ggcgacgatt gcggcgcgcg tccgctcagc agcggatcga tccggtgacg ctacggtgag
     6421 cggcaggcca tgctcggcca gcagccagag cagcgccgcg acgcccgggt cgagctcgcc
     6481 cagggcggtc agctcgcgca agctgggctg atcggaggtc attgcgtaag catcaggctt
     6541 tcgggtacga ttaaggcgaa gcccgtcgct acgggtgtaa tagccggtcc accacgacgc
     6601 tcctctcacg ggtcttacgc gagaactaag tcgcgaagcc cg
//
LOCUS       DATBRX010000135         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_156916, whole genome shotgun sequence.
ACCESSION   DATBRX010000135 DATBRX010000000
VERSION     DATBRX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156916"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VIF08_05180"
     CDS             <1..547
                     /locus_tag="VIF08_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8135415.1"
                     /translation="LRLADLGQVIGFVTQETFLFHATVRENLRYARPDATDEELDAAA
                     RMAAIHDRVSDLPQGYDTIVGERGYKLSGGEKQRVALARVLLKDPRILVLDEATSALD
                     TVSERLIQTAIERAMAGRTTIAIAHRLSTILRADLILVFDRGRIIERGRHDELLAHGG
                     LYARLYREQFLAATGAEPAPV"
     gene            complement(544..1377)
                     /locus_tag="VIF08_05185"
     CDS             complement(544..1377)
                     /locus_tag="VIF08_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135416.1"
                     /translation="MTRRHKLGILALALGAALALPIPAAAAHPIGLESGPGAGVEDLL
                     PDLRMADLYDIHLQRARSGDLRLRFGTIVWNLGRGPLEARIRGRDGREMTDVRQIIYR
                     SDGATRSVDRPNLFAFYSGDGHNHWHLPGFVVVLLTAIPQSGQPVPTEIRRLRKIGFC
                     LTDNFRAPDELRPPNSAPRAFYSWTGCGKRNATKLKMGISVGFGDEYKEWFAHQLVNV
                     TGVPAGSYRLCATPNQGGDWLESDNLNNSSWTDITMNPSTMTVTILRQGTGDCSVPPA
                     P"
     gene            complement(1430..2554)
                     /locus_tag="VIF08_05190"
     CDS             complement(1430..2554)
                     /locus_tag="VIF08_05190"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY8135417.1"
                     /translation="MTTEPISDSRPYSPGLKGVVAGETALAFIDGDKGRLLYRGYSIG
                     EIVEHGTYAQVAELLWTGYWSDEVHLGCAPLDHAVVEVLRQLPHTANAMDALRTGVSA
                     WGAITGCSFPPTVDQARAITAAAPSILAAFARLRDGREPIEPDPSLNLAAGFLYQLTG
                     ERPDAAAARGLEAYFMVGAEHGFNASTFTARVITSTQSDMASAVAGAIGALKGPAHGG
                     APTEVVNQLHEMGSVDQAERWIRATLDRKERLMGFGHRVYRAYDPRAAALRKVAEGLS
                     SVSEWLQMAVAVEEIALRVLHEYKPDYPIMTNVEYYAAAVLQGVGLPPNLFPATFALA
                     RHAGWTAHCLEQARADILIRPDVRYVGPAERGLPRVVART"
     gene            complement(2567..>3207)
                     /locus_tag="VIF08_05195"
     CDS             complement(2567..>3207)
                     /locus_tag="VIF08_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY8135418.1"
                     /translation="PIYDATTALIVVDVQNDFADPGGSLYVKTGEQVVPLINDEIARV
                     VAAGGRVFYTQDWHPESTPHFEKDGGIWPVHCVMGTWGSELHPGLSVGGPVVRKGSDG
                     EDGYSGFSMRDPVSGTTVPTALGDLLGEAGVTRLVVCGLATDYCVKATVLDGRERGYP
                     VTVLGIAIRAVDLQPGDGDRAVEAMFEAGALLESRAAVGEAGDAAGGAELGG"
BASE COUNT          503 a         1110 c         1110 g          484 t
ORIGIN      
        1 gctgcgcctc gccgatctgg ggcaggtgat cggcttcgtc acgcaggaga ccttcctgtt
       61 ccacgcgacc gtgcgcgaga acctgcgtta cgcgcggccg gacgccacag atgaggaact
      121 cgatgctgcc gcgcggatgg cggcgatcca cgatcgcgtg agcgacctgc cgcagggcta
      181 cgacacgatc gtcggcgagc gcggctacaa gctcagtggg ggcgagaagc agcgcgtcgc
      241 gctcgcacgc gtgctgctga aggatccgcg catcctcgtc ctcgacgagg cgacatcagc
      301 cctggacacc gtgtcggagc ggctgatcca gacggcgatc gagcgcgcca tggccggccg
      361 cacgacgatc gcgatcgccc atcgcctctc gaccatcctg cgcgccgacc tgatcctcgt
      421 cttcgaccgc ggacggatca tcgagcgcgg ccgccacgac gagctgctgg cccacggcgg
      481 cctctacgcc cgcctgtacc gcgagcagtt cctggccgcg accggcgccg agccggcccc
      541 cgtttagggg gcgggcggga ccgagcagtc gcccgtgccc tggcgcagga tggtgaccgt
      601 catggtcgag ggattcatcg tgatgtcggt ccagctcgag ttgttcaggt tgtcgctctc
      661 gagccaatcg ccgccctggt tgggagtggc gcagagccgg tagctgcccg ccggcacgcc
      721 ggtgacgttc acgagctggt gcgcgaacca ctccttgtac tcgtcgccga agccgactga
      781 gatgcccatc ttcaacttcg tcgcgttgcg cttgccgcag ccggtccacg aatagaacgc
      841 ccggggcgcg gagttgggtg ggcgcagctc gtcgggtgcg cggaagttgt ctgtcaggca
      901 gaaaccgatc ttgcgcagcc ggcgaatctc cgtgggtacc ggctggccgg actgcgggat
      961 cgcggtcagg aggacgacga cgaaacctgg aaggtgccaa tggttgtggc catcgcccga
     1021 atagaacgcg aagaggttcg gccgatcgac gctgcgggtt gcaccgtcgc tccggtagat
     1081 gatctgccgg acgtcggtca tctcacggcc gtcgcggccg cggatgcgtg cctccagcgg
     1141 gccgcggccc agattccaga cgatcgtgcc gaagcgcagt cgtaggtcgc cgctgcgggc
     1201 gcgctgcagg tggatgtcgt acaggtcggc catgcgcagg tcgggcagca aatcttccac
     1261 accagcgccc ggcccgctct caagtccgat cgggtgggcg gcagccgcag gaattggcag
     1321 ggcgagcgcc gcgccgagcg cgagcgccag gatccccagt ttgtgccgac gcgtcatggt
     1381 tgccggaggt tagccgccgc tcgcggctga gtccatcact tccgggcgac taagttcggg
     1441 ccacaacccg gggcagcccg cgctcggctg ggcccacgta gcgcacgtct ggcctgatga
     1501 ggatgtcggc ccgtgcctgt tccaggcagt gcgccgtcca gcccgcatgg cgcgccagcg
     1561 cgaacgtcgc cgggaagagg ttgggcggca ggcccacgcc ctggaggacg gccgcggcgt
     1621 agtactcgac attggtcatg atcgggtagt cgggcttgta ctcgtgcagg acgcgtagcg
     1681 cgatctcctc gaccgcgacc gccatctgca gccactcgct cacggaagaa agcccctcgg
     1741 cgaccttgcg cagcgcagcc gcgcgcgggt cgtacgcccg atacacgcgg tggccaaagc
     1801 ccatcagccg ctccttgcgg tcgagcgtgg cgcggatcca gcgctcggcc tgatcgaccg
     1861 agcccatctc gtgcagctgg ttgacgacct ccgtgggcgc gccgccgtgg gcggggccct
     1921 tcaaagcccc aatggcgccg gcaaccgctg aggccatgtc cgactgggtc gaggtgatta
     1981 cccgtgcggt gaaggtcgag gcgttgaaac cgtgctccgc gccaaccatg aaatacgcct
     2041 cgagcccgcg cgccgcagct gcgtcgggtc gctccccggt gagctggtac aggaacccgg
     2101 cggcaagatt gagcgacgga tcgggctcga tcggctcgcg cccatcgcgc agccgtgcaa
     2161 acgcggccaa gattgagggc gcagccgcgg tgatcgcgcg cgcctggtcg acggtcggcg
     2221 ggaagctgca cccggtgatc gccccccagg ccgagacgcc ggtgcgcagt gcatccatcg
     2281 cgttcgctgt atgcggcagc tggcgcagga cttcgacgac ggcatggtcg agcggcgcgc
     2341 accccaggtg tacctcgtcg gaccagtagc cggtccacag cagctcggcc acctgggcgt
     2401 acgtgccgtg ctcgacgatc tcaccaatgg agtagccgcg ataaagcagc cggcccttgt
     2461 cgccgtcgat gaatgccagt gcggtctcgc cggcgacaac gcccttgaga ccgggcgagt
     2521 acggccgact gtccgatatg ggctcggtcg tcatgcgtgg cgcagtctag ccgccaagct
     2581 ccgcgccgcc cgccgcgtca ccggcctcac ccaccgccgc acgcgactcg agcagcgccc
     2641 cggcctcgaa catggcttcc acggcccggt cgccgtcgcc cggctggaga tcaaccgcgc
     2701 ggatggcgat gcccaggacc gtcaccggat agccgcgctc gcggccgtcg aggacggtcg
     2761 ccttgacgca gtagtcggtc gcgaggccgc acaccacgag gcgcgtcacg ccagcctcgc
     2821 cgagcaggtc gccgagcgcc gtggggaccg tcgtgccact gaccgggtcg cgcatcgaga
     2881 agcccgagta gccgtcctcg ccgtcgctgc ccttgcgcac gaccgggcca ccgaccgaca
     2941 ggcccggatg cagctcagaa ccccatgtgc ccatcacgca atggacgggc cagatgccgc
     3001 cgtccttctc gaagtgcggc gtggactcgg ggtgccaatc ctgggtgtag aagacgcgtc
     3061 ccccggccgc cacgacgcgc gcgatctcgt cgttgatgag cggaaccacc tgctcacccg
     3121 tcttgacgta gagcgagcca cccggatcgg cgaagtcgtt ctggacgtcg accacgatga
     3181 gcgcggtcgt cgcgtcgtag atgggca
//
LOCUS       DATBRX010000136        16884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3286527, whole genome shotgun sequence.
ACCESSION   DATBRX010000136 DATBRX010000000
VERSION     DATBRX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16884
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3286527"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..2751
                     /gene="aceE"
                     /locus_tag="VIF08_05200"
     CDS             16..2751
                     /gene="aceE"
                     /locus_tag="VIF08_05200"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY8135419.1"
                     /translation="MFDEFGDFKRQLPDRDADETNDWVHSLDSVIADEGKARAQFILY
                     RLLKRARQHDLGLPPLTATRYINTISPEQEPPFPGDEMIERRIRRMIRWNAVAMVLRA
                     NNRAPGIGGHLATYASAASLYEVGFNHFFRGKDAEGMGDQIFFQGHAAPGIYARAFLE
                     GRLSADRLDHFRQEVVPGQGLSSYPHPRLMPDFWEFPTVSMGLSPLAAIYQARFNRYL
                     EHRGIKDTSHNRVWAFLGDGETDEPESLGALSVAAREGLDNLTFVVNCNLQRLDGPVR
                     GNGKIIQELESVFRGAGWNVIKVIWGREFDPLLANDVDGVLVDKMNETLDGEFQKYSV
                     ESGAYIREHFFGPDPRLQQLVAHLSDDDIAHLRRGGHDYTKVYAAYDAATKTKGRPTA
                     ILAKTVKGWTLGTGVEGRNITHQAKKLTEQELKTFRDRLELPISDADLKDAPYYYPGD
                     DAPEIEYLRERRATLGGPMPRRVVVTKSIELPGDPVFGEFMTGSGSQEVSTTMAFAKL
                     LRNLLRDKAIGPRIVPIIPDEARTFGMDPLFKEVGIYSALGQLYEPVDSNLVLSYREA
                     TDGQVLEEGITEAGSSASFQAAGTSYSTHGEPMIPLYIFYSMFGFQRTGDEFWAFADA
                     RGRGFLLGGTAGRTTLNGEGLQHEDGHSLILASVIPTIRVYDPAFAYETAVIIREGIN
                     RMYGENPEDVLYYLTLYNENYAMPARPEGVSDADITRGLYLFRAAPEIAAPSGNGNGR
                     EAHPVLTVTLLAGGSIMQQALRAQAMLAERGVAADVWSATSYQLLRRDALETMRWNYL
                     HPDEEVRLPLVSQLLAEPAARGPIVAVSDFVTDWPDMISRWIPGSSYISLGTDGFGRS
                     DTREALRRFFQVDADHITAAALSELARAGKLDAGEARQHIRDLGLEKEEAFTVSA"
     gene            2817..4265
                     /locus_tag="VIF08_05205"
     CDS             2817..4265
                     /locus_tag="VIF08_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8135420.1"
                     /translation="MQATGVVIAADGPSAAEVNSFSLRQADGVVLNFRINRLDLSNGG
                     LPSAHLREHMASGEPITVDYHVENGLYIADRYTDAAEIETQQPSQQPTTTSGPGTLPP
                     PTATATAVVTPPPTPTPEPTPVAPFHLGIEPFVDGFEALTFLTNAADFTGDLYAVEQR
                     GVIWRIGADGTVDPTPFLDIHERVSCCGERGLLGLAFHPGYAENDRFFVNYTDNAGNT
                     VVSEFGAPDDGYGDADSERRLLGINQPFPNHNGGMLAFGPDGYLYIGTGDGGSGGDPM
                     GNGQDLTALLGKILRIDIDSGDPYGIPADNPFVGQIPEVPHRPEIWDRGMRNPWRFSF
                     DRQTGDLWIGDVGQDIYEEVDAEPAGTGGRNYGWNTMEASHCFATPNCDQAGLTLSVA
                     EYRHAQGCSITGGYVYRGSALPQLIGQYVFADYCSGRIWALDAATALSAGSAEPTQYG
                     KVPINPTSFGQDEAGELYLVDGAGGIYRLIVE"
     gene            4281..5123
                     /locus_tag="VIF08_05210"
     CDS             4281..5123
                     /locus_tag="VIF08_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8135421.1"
                     /translation="MNPIVARYDTDAADYGRYWAPVLEETARRLLEYVEPFVAKRGGA
                     VRMLEVGAGTGTLLLAALERWPRAEFIATEPARGMLDLAESRVRAARPGESRVTFLEG
                     LADALPVDDSSVDLVISSFVLQLVPDRLAALREAARVIKPTGMVSYVTWLDRDSRRPF
                     IPAEEFDEAVYALQVEEPEGADEPHAGDVRSGRVATDELRRAGFVRASATEEELIYGW
                     TPQTYLDYKLAYDERSLMRSLKAPQRKQLEKNARERLGRLKERDFRWHAPVVFARALK
                     ATGS"
     gene            complement(5120..5794)
                     /locus_tag="VIF08_05215"
     CDS             complement(5120..5794)
                     /locus_tag="VIF08_05215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8135422.1"
                     /translation="MPRFVDLSHEISDGLVTYPGLPAPQIDEYLSRVASRARYAPGTE
                     FSIGRITMVANTGTYLDTPFHRFADGFDLTGLSLEQLVDLDGVLVRVVGASERAIGAD
                     RFTGLDVNGKAVLVHTGWDRHWATPEYGVGHPFLTATGAAWLAEHGAAIVGIDSLNID
                     DGADGSRPAHTTLLGAGIPIVEHLTGLDQLPSTGFRFTAVPARVAGMGTWPVRAFARI
                     EDGAPT"
     gene            complement(5804..6619)
                     /locus_tag="VIF08_05220"
     CDS             complement(5804..6619)
                     /locus_tag="VIF08_05220"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8135423.1"
                     /translation="MTDKQVEAGLRSLRTPAPQGFGSKVLDRLGLGDAYVRVEGPIGP
                     LFVAFGRDGISLVERADGTHDAGDFEAMFRATFGRPVRRIERAPELMERVIDARLWGK
                     TKLAGKVPVDLHELPEFEQQVLRKVLEIPRGEVRPYAWVAAELGRPLAVRAVGNAVAR
                     NPIPFVIPCHRVVRSDGRIGNYGAGGPTAKRAVLESEGMDTTEMERLPAKGVRFIGSD
                     TTNIYCYPSCRNARRITARHRVALRSADDARHAGFRACKVCRPVDPPFRAAVA"
     gene            complement(6636..7211)
                     /locus_tag="VIF08_05225"
     CDS             complement(6636..7211)
                     /locus_tag="VIF08_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8135424.1"
                     /translation="MAIATTDPLCLRLADDLDQAFPELVTDHQGLVFGVAVRVVGDSN
                     AEDVAQEVFVRAYRALKRYPAARVREMRLRPWLARMTLNQARNALRGRRDHVDIADVA
                     ESRQSNEPGPAQLVQRAEEQSMWRRMLDGLPARYRLTVALRHVDELSYAEIAETLGRP
                     LGSVKSDVHRGIALLRAAYDAEQRATSQEAV"
     gene            complement(7509..9158)
                     /locus_tag="VIF08_05230"
     CDS             complement(7509..9158)
                     /locus_tag="VIF08_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEY8135425.1"
                     /translation="MSQQPIVRHYRGATRAEVEAAYRADAQPAIAAGYLPMGHIWSQD
                     VRGLLLAVTYQPHSVPAAAPIAASQSRQYVAQPERVAQPEYVAQPEYIAQPQHHPGQF
                     APATNGSTGSALGSQWSAPVQPQARYQPPPEMYMAPPDMYAPAAAPVAPTPVAAAPAP
                     APTSAPAPVAPTPSVTPAPAPANGYGQQEAASSRQTRLTVQTMDLHTAGEPLRIIRSG
                     FPDVPHMPVLERRRWVLENADYVRRAIIQEPRGHRDMYGAILLPPYNDYADMTVLFMH
                     NEGYSTMCGHGIIAITTGLIEEGLFPANEPLTTIRYEVPAGIVAANAATVQLDDGSWA
                     VRGVRFTNVPSYLAAQSLAVRPDGVQLYGPAAQYGALSVDIAFGGAYYGIVNAADLGL
                     RVVPEQAEALRHAGAQITEVLRRDHTPQHPTDPALGFVYGTIIVDMDPRTSPDGRATS
                     AHLRNVTVFADAEVDRSPCGSGTSALLAQLHARGRMRVGQEIVNAGITGEHFLGRIEA
                     ETALGPYRAVSTSIAGTAFVTGYSTFVVDSRDPLGDGFLLG"
     gene            9483..10148
                     /locus_tag="VIF08_05235"
     CDS             9483..10148
                     /locus_tag="VIF08_05235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY8135426.1"
                     /translation="MDRWNAVDDYIVDTFDLDDDVLRAAVEASVAAGMPQIQVSAALG
                     RFLNLQARAIGAHRILEVGTLAGYSSIWLARALPADGRLVTLELSPKHADVARGNLAR
                     AGLDAVAEVRVGPATDSLAALVADKVEAFDMVFIDADKEGYPDYLEWSLRLSRPGTLI
                     VADNVVRGGAIIEPDHADSRVRGIRRFNEMLARDHRVNATIMQTVGAKGYDGIAFAVV
                     LDR"
     gene            10160..11416
                     /locus_tag="VIF08_05240"
     CDS             10160..11416
                     /locus_tag="VIF08_05240"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8135427.1"
                     /translation="MARRLPLLALYSADAISVTGNVMALVAIPWFVLELTGSAALTGV
                     AAFFSTLAAVVAAFFGGTLVDRVGFKAMSVISDIASSVAIAAIPALYLTVGIQPWQLM
                     VLVFLGALLDAPGTAARSSMLPDLAALSGTPLERATSLSQAVRRGATLVGAPLAGALI
                     VVVGTHNVLLLDAVSFLFSAALVGLLTPSLTHHEATAEGEEPQPRGRYFDELLAGLRF
                     VWRDRLVRAIVLTVMITNFLDAPLLSVVLPVYVHQVYGSSVDLGLLFGAFGGAALAGA
                     VGYGLIGPRLPRRLTFAIAFVIVGLPMWLLAFQPPFVVALLGFVVIGLAAGPINPIIS
                     TVILERVPRSMRGRVTGTITSGAFVAMPAGMLIAGFVSQLYGVITAIVLIAAAYVVVT
                     LSIFVNPAMREMDRIPSGAQASRPAR"
     gene            complement(11392..11733)
                     /locus_tag="VIF08_05245"
     CDS             complement(11392..11733)
                     /locus_tag="VIF08_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEY8135428.1"
                     /translation="MKLEGTGLLVRIYLGESDQWHGRPLYQAIVERLRERGLAGATVL
                     RGIEGFGAKQHLHSTRILSLSQDLPILIEVVDTEEKVRAILPELDEMLADGLMTLEKV
                     EVIAYRAGRDA"
     gene            complement(11730..12104)
                     /locus_tag="VIF08_05250"
     CDS             complement(11730..12104)
                     /locus_tag="VIF08_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEY8135429.1"
                     /translation="MVGVLLIGAGGFAGAVARYLVDAEVSQRFASFPWGTLIVNVSGS
                     FLVGLLFALITERAALPAELRGPLMIGFLGAYTTFSTLTLESWRMIEDGAWLLAALNL
                     GGSVVIGMIAVIGGLALGRAIS"
     regulatory      complement(12126..12186)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(12191..13504)
                     /locus_tag="VIF08_05255"
     CDS             complement(12191..13504)
                     /locus_tag="VIF08_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8135430.1"
                     /translation="MAWTRLATRCRRRSPLAEPGPAAYDAVDRIFREEQGRVVAGLIR
                     AVGDIDLAEESVQDAFIAALEHWPRDGLPPNPGAWIATTARNRAIDRLRRAKRFTEKT
                     EELARLMTTEGAYQEPGPDMLTDRPAVEDDRLRLIFTCCHPALAADAQIALTLRLLGG
                     LRTPEIARAFLLPEATLAQRLVRAKRKIRDAAIPYVVPAAEALPERLGSVLHVLYLVF
                     NEGYSATAGATVVRRELASEAIRLARLVVALMPGESEALGLLALLLLQDSRRDARQDA
                     QGDLVLLADQDRSRWDRARITEGLAVLDRAERLGPRGPYVLQALIAAEHVRAAAAADT
                     DWPRIVSLYDELVAAQPSPVIALNRAVAVSMASGPAVGLALVDAMAASGELAGYHLLH
                     STRGELLRRVGRLEDARAALSRARELATNDVDRRYLDRRLGELAH"
     gene            complement(13452..13862)
                     /locus_tag="VIF08_05260"
     CDS             complement(13452..13862)
                     /locus_tag="VIF08_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8135431.1"
                     /translation="MRYALLIYVEPWESTPEQDVEVMNAYNGFTRAAVAADVMRGGEA
                     LHEAKTATSVRVRDGQTLVTDGPFAETKEEFGGFYLIEAENLDEAIKWAAQIPGAVRG
                     TIEVRPVVDFPSPGNPGDGMDAAGNPVPQTIAAG"
     gene            13968..14219
                     /locus_tag="VIF08_05265"
     CDS             13968..14219
                     /locus_tag="VIF08_05265"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEY8135432.1"
                     /translation="MPTYLDTHRHVPGLTAAAVADAHAKDMEVGPKYGVTYVRYWFDE
                     ATGRVYCLVEAPSPELAEQVHREAHGLLADEIVAVVEGA"
     gene            complement(14224..>16884)
                     /locus_tag="VIF08_05270"
     CDS             complement(14224..>16884)
                     /locus_tag="VIF08_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8135433.1"
                     /translation="LRDAYGPLLEAYHDLIEAVFTKHGGTQLDQAGDGLFHSFPSSRS
                     AASAAIEAQLAMAERDWPGGASVRARMGIHTGEAVTRRSGYVGMDVHRAARIAAAGHG
                     GQVLLSQVTRDLVATDLPAEAVLSDLGEHWLKDLAQPEHLYQLTIQGLPQAFPPLKSL
                     VTLPNNLPRHLNSFVGRQGDVAEVRRLTTEAPLVTLTGPGGVGKTRLAIQVAAELLDT
                     FADGAWLVELETLTDENLVAQQVAVALGIAEASDADAQESVLGHLRTRQALLILDNCE
                     HVIEACARLANALLRGCPGLRIIATSREALGVSGERMYPVRSLTLPTSARTVSAAVAA
                     EFEAVDLFCERARAADPSFTITDDNAPAVVEICERLDGIPLAIELAAARVRALSPQQI
                     AERLDDRFRLLTGGSRTVMPRHQTLRAAIDWSFDLLPETERSVLWRLSVFSGAFALEA
                     AEAVCVGTDVASEEVIDHVTRLVEKSLVNRQQDRYRLLETVRGYARERSLEAGEAAGA
                     YERHRDWYLEFVRRAAPAFFRGPESAESLDALEAQHDNLRTALSWSLNEAGGASAALE
                     LASGLWRFWEIRGYLREGRQWLERVLAATPGDSSTTRADVLTAAGILAAAQGDHAAAV
                     RYHEQSLAVQEQLGSKQGVQFALHNLANAAMHTGDLGQARTLYERAIAIAGTEDPNRA
                     FVYVNLADVADSQGDAQAARGYYEQAIEIVAASGDIWGMAYAQSSYGQSAARQSDHAA
                     ARQRYEQALAIYRQLGDQRGEARLMTLLADVDADEGKAAEALRLLYGALEIRCRLGDS
                     PGICGALERFAAGAMDLDATRAARILAAATSLRDRTGARLSPAGQAAMDQQLARLQRV
                     LGAEFGAAWQSGSGAGVDEALREAEAVLSR"
BASE COUNT         2673 a         5903 c         5634 g         2674 t
ORIGIN      
        1 taaactggcc cgagcatgtt cgacgagttc ggcgacttca agcgccagct gcccgaccgc
       61 gacgcggacg aaaccaacga ctgggttcat tcactcgact cggtcatcgc cgacgagggc
      121 aaggcgcgtg cgcagttcat cctctatcga ctgctgaagc gcgcgcgcca gcacgacctg
      181 ggcctaccgc cgctgaccgc gacccgctac ataaacacga tctcgcccga acaggaaccg
      241 ccgttcccgg gcgacgagat gatcgagcgc cgcatacggc gcatgatccg ttggaacgcg
      301 gtggccatgg tgttgcgcgc caacaaccgt gcgcccggca tcggcggcca tctcgcgacc
      361 tatgcgagcg cggcatcgct gtacgaggtc ggcttcaacc acttcttccg cggcaaggac
      421 gcggaaggga tgggcgacca gatcttcttc cagggccacg cggcgccggg catctacgcc
      481 cgcgccttcc tcgagggtcg gctctccgcg gatcggctcg atcacttccg ccaggaggtc
      541 gtccctggcc agggactgag ctcatacccg cacccgcggc tgatgcccga cttctgggag
      601 ttcccgacag tgtcgatggg cctctcgccg ctggccgcga tctaccaggc gcggttcaac
      661 cgctacctcg agcatcgcgg gatcaaggac acgtcgcaca accgggtgtg ggcgttcctg
      721 ggtgatggcg agaccgacga gcctgagtcg ctcggcgccc tcagcgtcgc cgcgcgcgag
      781 ggcctcgaca acctgacctt tgtcgtgaac tgcaacctgc agcgcctcga tggcccggtg
      841 cgcggcaacg gcaagatcat ccaggagctc gagtccgtct tccgcggcgc aggctggaac
      901 gtgatcaagg tcatctgggg ccgcgagttc gacccgctgc tcgccaacga tgtcgacggc
      961 gtcctggtgg acaagatgaa cgagacgctc gatggcgagt tccagaaata cagcgtcgag
     1021 agcggcgcgt acatccgcga gcacttcttc ggccccgatc cgcgcctgca gcagctcgtc
     1081 gcccatctgt cggacgacga catcgcgcac ctgcgccgcg gcggccacga ctacacgaag
     1141 gtctacgcgg cctacgacgc cgcgacgaag accaagggcc gcccgacggc gatcctggcc
     1201 aagacggtca agggctggac cctgggcaca ggcgtcgagg gccgcaacat cacccaccag
     1261 gccaagaagc tgaccgagca ggagttgaag acgttccgcg accggctgga gctgcccatc
     1321 tccgacgcgg atctcaagga cgccccgtat tactacccgg gcgacgacgc gcctgagatc
     1381 gagtacctgc gcgaacggcg ggcgaccctg ggcggtccga tgccgcgccg cgtcgtggtc
     1441 acgaagagca tcgagctgcc cggtgacccg gttttcggcg agttcatgac cggctcgggc
     1501 agccaggaag tcagcaccac gatggccttc gccaagctgc tgcgcaacct gttgcgcgac
     1561 aaggccattg gcccgcgcat cgtgccgatc atccccgacg aggcgcgcac cttcggcatg
     1621 gacccgctct tcaaggaagt gggcatttac tcggccctgg gccagctgta cgagcccgtc
     1681 gactcgaacc tggtcctgtc gtatcgcgag gccacggacg gccaggtcct cgaggagggg
     1741 atcaccgagg ccggttcatc ggcctccttc caggccgccg gcacgtcgta ctcgacccac
     1801 ggcgagccga tgatcccgct ctacatcttc tattcgatgt tcggcttcca gcgcaccggc
     1861 gacgagttct gggcattcgc cgacgcccgc gggcgtggct tcctgctggg cggcacggcc
     1921 ggcaggacga cgctcaacgg ggaggggttg cagcacgagg acggccattc gctcatcctc
     1981 gcaagcgtca tccccaccat ccgcgtctat gaccccgctt tcgcctacga gaccgcggtc
     2041 atcatccgcg agggcatcaa ccgcatgtac ggcgagaacc ccgaggatgt cctctactac
     2101 ctcacgctct acaacgagaa ctatgcgatg cccgcgcggc ctgagggcgt aagcgacgcg
     2161 gacatcacgc gcggcctgta cctgttccgc gccgccccgg agatcgccgc gccaagcggc
     2221 aacggcaacg gccgcgaggc gcacccggtg ttgacggtga cgctgctggc cggcggatcg
     2281 atcatgcagc aggcgctgcg cgcccaggcg atgctcgccg agcgcggtgt cgcggccgat
     2341 gtctggagcg cgacgtccta ccagctgctg cggcgcgacg ccctcgagac gatgcgctgg
     2401 aactacctcc accccgacga ggaggtgcgc ctgccgctcg tttcgcagtt gctggcggag
     2461 cccgccgcgc gcggtcccat cgtcgcggtg agcgacttcg tgacggattg gccggacatg
     2521 atctcgcgct ggatcccggg tagctcgtac atctcgctgg gcactgacgg cttcggccgc
     2581 tctgacacgc gcgaggcgct gcgccgcttc ttccaggtgg acgcggacca catcaccgcc
     2641 gccgccctat ccgagctcgc gcgcgccggc aagcttgacg cgggcgaggc gcgccagcac
     2701 atccgcgacc ttggtctcga aaaggaagag gcattcacgg tcagcgcctg atcggcaccc
     2761 ttctggcgat cgcgctgctc agtgcggcct gcgcagccac gccgaccgcg tcgcccatgc
     2821 aggccacggg cgtggtcatc gcagccgacg gtccatcggc cgcggaagtc aactcgttct
     2881 cgttgcgcca ggccgacggc gtcgtgctca atttccggat caatcgcctt gacctgagca
     2941 atggtggcct gccatccgcg cacctgcgcg agcacatggc gtcgggcgag ccgatcacgg
     3001 tcgactacca cgtcgaaaac ggtctctaca tcgccgaccg atacaccgac gctgccgaaa
     3061 tcgagaccca gcaacccagc cagcaaccga ctaccacttc gggtcccggc acactgcccc
     3121 cgccgactgc cacggccacg gcggtcgtta cgccgccacc gaccccgaca cctgagccga
     3181 cgccagtggc gcccttccac ctgggcatcg agccattcgt cgacggcttc gaggcgctga
     3241 ccttcctgac aaatgcggcc gacttcacgg gcgatctgta cgcagtcgag cagcgcggtg
     3301 tgatctggcg catcggcgct gacggcacgg tcgatcccac gcctttcctg gatatccacg
     3361 agcgcgtcag ctgctgcggt gagcgtggcc tgttgggact ggccttccac ccgggctacg
     3421 cggagaacga ccggttcttt gtcaattaca ccgacaacgc cggcaacacc gtcgtttcgg
     3481 agttcggtgc accagatgac ggatacggcg acgccgactc cgagcgacgg ctgctcggca
     3541 tcaaccagcc cttccccaac cacaacggcg gcatgctcgc ttttggcccc gatggctacc
     3601 tgtacatcgg cacgggtgac ggcggctcgg gcggcgatcc catgggcaat ggccaggacc
     3661 tgacagccct gctcggcaag atcctgcgca tcgatatcga tagcggcgat ccgtacggca
     3721 tcccagcaga caacccgttc gtcggccaaa tccccgaggt cccacaccgg cccgagatct
     3781 gggaccgggg catgcgcaat ccctggcgct tctccttcga tcgccagacg ggcgatctgt
     3841 ggatcggcga tgtcggccag gacatctacg aggaggtcga cgccgagccg gccggcacgg
     3901 gcggtcgcaa ctacggctgg aacacgatgg aagcgagtca ctgcttcgcc accccgaact
     3961 gcgaccaggc cgggctgacg ctgtccgtgg cggagtaccg gcacgcgcag ggctgctcga
     4021 tcacaggtgg ctacgtctat cgcggcagtg cgctgccgca gctcatcggc cagtacgtct
     4081 tcgccgacta ctgctcgggc aggatctggg cgctcgacgc tgcgacggcg ttgtcggccg
     4141 gctccgccga accgacccag tacggcaagg tacccatcaa cccgaccagc ttcggccagg
     4201 acgaggcggg cgagttgtac ctggtcgatg gcgcgggcgg gatctaccgg ctgatcgtcg
     4261 agtagctcgc cgcgaccctg atgaatccga tcgtcgcgcg ctacgacacg gatgccgccg
     4321 attacggccg ctactgggcg ccggtactcg aagaaaccgc gcgccggctg ctggagtacg
     4381 ttgagccgtt cgtcgccaag cgcggcggcg cggtgcggat gctcgaggtg ggcgcgggca
     4441 ccggcacgct cctgcttgct gcgctggagc gctggccgcg cgccgagttc atcgccacgg
     4501 agcctgcacg cggaatgctc gaccttgccg aatcgcgtgt ccgggccgcg cggccgggcg
     4561 aatcgcgcgt caccttcctc gagggtctcg cagacgcgct tccggtcgat gacagctcgg
     4621 ttgacctcgt catctcctcg ttcgtgctgc agctcgtccc ggaccgtctc gccgccctgc
     4681 gcgaggccgc tcgtgtcatc aagccgaccg ggatggtcag ctacgtcacg tggctcgacc
     4741 gcgactcgcg ccggccgttc atcccggccg aggaattcga cgaggcggtc tatgccctgc
     4801 aggtcgagga gccggagggc gccgacgaac cgcatgccgg tgatgtccgc tccggccggg
     4861 tagcgaccga cgagctgcgt cgcgccggtt tcgtgcgcgc gtcagccacg gaagaggagc
     4921 tcatctatgg ctggacgccc cagacgtatc tcgactacaa gctcgcgtac gacgagcgct
     4981 ccttgatgag gtcgctcaag gcgccccagc gaaagcagct ggagaagaac gcgcgcgaac
     5041 ggctcggtcg cctgaaggag cgcgacttcc gctggcacgc accggtggtc ttcgcgcgcg
     5101 ccctgaaagc cactggctcc taggtagggg cgccatcctc gatgcgcgcg aaagcgcgaa
     5161 ccggccacgt gcccatgccg gcgacacgtg ctggaacggc ggtgaagcgg aagcccgtcg
     5221 acggcagctg atcgagtccc gtcaggtgtt ccacgatcgg gatacctgcg cccagcagag
     5281 tcgtatgagc cggtcgcgac ccatctgcgc cgtcatcgat gttgagcgaa tcgatgccca
     5341 cgattgccgc tccgtgctct gccagccatg ctgcgcccgt cgcagtcagg aatggatggc
     5401 ccacgccgta ttccggcgtt gcccagtgac ggtcccagcc ggtgtgaacc aggaccgcct
     5461 tgccgttgac gtccagcccg gtgaatcggt cggccccgat cgcgcgctcc gaagccccga
     5521 ctacccgcac cagcacacca tcgagatcga ccagctgctc caggctcaga ccggtcaggt
     5581 cgaagccatc ggcgaaacgg tggaacggcg tatcgaggta ggtgcccgta ttggccacca
     5641 tcgtgatccg gccaatgctg aattcggtcc cgggcgcgta acgagctcga gacgcgaccc
     5701 tgctcaggta ttcatcgatc tgcggcgcgg gcagcccggg gtaggtgacc agcccgtcgc
     5761 tgatctcgtg cgacaggtcg acgaatcgcg gcatgccggc aggctacgcg acggcggcgc
     5821 ggaatggtgg atcgaccggc cggcagacct tgcaggcgcg gaagccggcg tgacgcgcgt
     5881 cgtcagcgga acgcagcgcg acgcggtgcc gagcagtgat ccgtcgcgcg ttgcggcacg
     5941 acgggtagca gtagatgttg gtcgtgtcgg agccgatgaa gcgcacgccc ttggccggca
     6001 gacgctccat ctctgtcgtg tccatgccct ctgactccag gacggcccgc ttcgcggttg
     6061 ggccaccggc gccgtagttg ccgattcgcc cgtcgctgcg cacgacgcga tggcacggga
     6121 tcacgaaggg aatggggttg cgcgcgacgg cattgcccac cgcgcggacc gcgagtggcc
     6181 ggccgagctc ggccgccacc caggcatacg gccgcacctc gccgcgcggg atctcgagca
     6241 ccttgcgcag gacctgctgc tcgaactcgg gcagctcgtg caggtcgacc ggcaccttgc
     6301 ccgcgagctt cgtcttgccc cacaggcgcg cgtcgatgac gcgttccatc agctccggcg
     6361 cgcgctcgat gcgtcgcacc ggccggccga acgtggcccg gaacatggcc tcgaagtcac
     6421 ccgcgtcgtg cgtgccgtcg gcgcgctcca cgagcgagat gccgtcgcgc ccgaaggcga
     6481 cgaagagcgg cccgatcggg ccctcgacac gcacgtacgc gtcccccaac ccaagtcgat
     6541 ccagcacctt gctcccaaat ccttggggcg cgggtgtccg cagcgaacgg agtcccgcct
     6601 ccacctgctt gtcggtcatc tgccgagtta ccacctcaaa ccgcctcctg cgacgtggca
     6661 cgttgctccg cgtcatatgc ggcgcgcagc agcgcgatgc cgcgatgaac gtcagatttg
     6721 acgctcccca atggcctgcc gagcgtctcc gcgatctccg cgtacgacag ttcgtccacg
     6781 tgccgtagcg ccaccgtcag gcgatagcgc gccggaagcc cgtcgagcat ccgccgccac
     6841 atgctctgct cctcagcgcg ttggaccagc tgcgcgggtc ccggctcgtt ggactgccgc
     6901 gactccgcta catcagcgat gtcgacgtgg tcccgccgtc cacgcagcgc attgcgcgcc
     6961 tggttgagcg tcatacgcgc gagccacggc cggaggcgca tctcgcggac acgcgccgcc
     7021 gggtagcgct tgagcgctcg gtacgcacgc acgaacacct cctgggctac gtcctcggca
     7081 ttggagtcac cgactacccg cactgccact ccgaacacca gaccctggtg gtcggtgacc
     7141 agctcgggaa aggcctggtc gaggtcatcg gccaagcgca aacagagcgg gtcggtcgtg
     7201 gcgatcgcca tgtacacgcc ggaataacac agcccgcgcc agatcggttg cgaatttgac
     7261 gcccggcaga attctgacgt tcctgggacg tcaaatgtcc gctagaccac agcgattcgc
     7321 cggcgcaagc cacatcacgc gacgctccgc gacccagcta tgcggctggc cgcatcgaat
     7381 gggggtagag cggcctcgtg atggcctgcc agcctggtat ggggcacgtg atccggcgaa
     7441 cttcggacat ttgacgtgtg tggcgcatca cggatgaggg cgcggctcgg gggccgcgac
     7501 tcggggtcct agccgaggag gaagccgtcg cccaacgggt cgcggctgtc gacgacgaag
     7561 gtcgagtaac cggtcacgaa tgccgtgcct gcgatggaag tcgaaacggc gcggtagggc
     7621 cccagtgccg tctcagcctc gatgcggcca aggaagtgct cgccggtgat gccggcgttg
     7681 acgatctcct ggcccacgcg catccggccg cgggcgtgca gctgggcgag cagcgcgctc
     7741 gtgcccgaac cgcaggggga gcgatcgacc tcggcgtcgg cgaacacggt gacgttgcgc
     7801 aggtgcgcgc tcgtggcgcg gccgtcgggc gaggtgcgcg ggtccatgtc gacgatgatc
     7861 gtgccgtaga cgaagccgag cgccggatcg gtcgggtgct gcggcgtgtg gtcgcggcgc
     7921 aggacttcgg tgatctgtgc gccagcgtgg cgcagcgcct cggcctgctc gggcacgacg
     7981 cgcaggccga ggtcagccgc gttgacgatg ccgtagtagg caccgccgaa tgcaatgtcg
     8041 acgctcaagg cgccgtactg ggcggccggt ccgtagagct ggacgccgtc aggtcgcacg
     8101 gccagcgact gcgcggcaag gtatgagggc acgttggtga agcgcacacc gcggacggcc
     8161 cacgagccgt cgtccagctg gacagtcgcc gcattggcgg cgacgatgcc cgccggcacc
     8221 tcgtagcgaa tggtcgtgag gggctcgttc gccgggaaga ggccctcttc gatgagaccg
     8281 gtcgtgatgg cgatgatgcc gtgcccgcac atcgtcgagt agccctcgtt gtgcatgaag
     8341 agcacggtca tgtcggcgta atcgttgtac ggcggcagga ggatcgcgcc gtacatgtcg
     8401 cgatggccgc gcggctcctg gatgatcgcg cgccgaacgt agtcggcgtt ctcgaggacc
     8461 cagcggcggc gctcgagcac cggcatgtgc ggcacatcgg ggaagccgct gcggatgatg
     8521 cgcagcggtt cgcccgcggt gtggagatcc atcgtctgga cggtcagtcg ggtctggcgc
     8581 gacgatgcgg cttcctgctg gccgtagcca ttcgcggggg cgggcgccgg agtgacagag
     8641 ggggtcggtg cgacgggggc aggggccgac gtgggcgccg gggcgggtgc cgctgcgacc
     8701 ggggtcgggg cgactggtgc agccgcgggc gcgtacatgt ccggcggcgc catgtacatc
     8761 tcgggcggcg gctggtatcg cgcctggggc tggaccgggg cggaccactg cgagccgagt
     8821 gccgagccgg tgctgccatt ggtggcgggg gcgaactgac ccgggtgatg ctggggctgg
     8881 gcgatgtact ccggctgggc gacgtactcc ggctgcgcga ccctctccgg ctgcgcgacg
     8941 tactgccgcg actggctggc tgcaatcggg gccgccgccg gaacggagtg gggctggtag
     9001 gtgacagcga gcagcaggcc gcggacgtcc tgtgaccaga tgtggcccat gggcagatag
     9061 cccgcggcaa ttgcaggctg agcgtcggca cggtaggcgg cctcaacctc cgccctggtg
     9121 gcgccgcggt agtggcgaac gatcggctgc tgactcactc ttggctccaa accggcgacc
     9181 actctagtgc ctcaaaccgg aacgagccgc agtgcgccga agcaccgcac aaaaccggag
     9241 cgaaccatcc cattgctccc caatcgtgac cagttgccgc gtccgacggc tctcgcccga
     9301 agtactgccg ggtctggaac gatggtcccc gggcgaccgc gaacgtacac gcgaaatggg
     9361 cctccgacca cccccttgga ggccttcgtg caccgattgc gttgaccgcc cgcacccttc
     9421 tcaaaccgca cccccgcggc cccggaaccc gcacagtggc ctcttctggg tatgctgccg
     9481 cgatggaccg ctggaatgcc gtcgacgact acatcgtcga caccttcgac ctggacgacg
     9541 acgttcttcg cgccgccgtc gaggcgagcg tcgccgccgg catgccgcag atccaggtgt
     9601 cggcagcgct cgggcgcttc ctgaacctgc aggcgcgggc gatcggcgcc catcggatcc
     9661 tggaggtcgg cacgctggcc ggctactcgt cgatctggct ggcccgcgca ctacctgccg
     9721 atggccgcct ggttacgctc gagctctcac ccaagcatgc cgatgtggcg cgtggcaacc
     9781 tggcgcgggc gggactcgac gcggttgcgg aggtgcgcgt tgggccggcc accgactcgc
     9841 tcgcggcgct cgtcgcggac aaggttgaag cgttcgacat ggtcttcatc gacgccgaca
     9901 aggagggcta ccccgactac ctcgagtggt cgcttcgcct gtcgcggccg ggcactctga
     9961 tcgtggccga caacgtcgtc cgcggcggcg cgatcatcga acccgaccat gccgacagcc
    10021 gcgtccgggg catacgccgc ttcaacgaaa tgctcgcccg cgaccaccgc gtgaacgcga
    10081 ccatcatgca gaccgtgggc gccaagggct acgacggaat cgccttcgcc gtcgtcctcg
    10141 accgctagcc ttgcgcgcga tggcgcgccg cctgccgctc ctggccctct attcggccga
    10201 cgcgatatcg gtcaccggga acgtcatggc cctggtggcc atcccatggt tcgtactcga
    10261 gctgaccggc agcgccgcgc tgacgggcgt cgccgccttc ttcagcacgc tggccgctgt
    10321 ggtggctgct ttcttcggtg ggacgctcgt cgaccgcgtt ggcttcaagg cgatgtccgt
    10381 catatcggac atcgcctcct cagtggcgat cgccgcgatc ccggcgctct atctgacggt
    10441 cggcatccaa ccgtggcagc tcatggtgct ggtcttcctg ggtgcactgc tcgacgcccc
    10501 gggcacagcc gcgcgaagct cgatgctgcc cgatctcgcc gcgctatccg gcacgccgct
    10561 ggagcgcgcg acgagtcttt cgcaggcagt ccgccgcgga gcgactctcg tcggtgcgcc
    10621 gctcgccgga gcgctcatcg tggtcgtggg cacgcacaac gtgctgctgc tcgacgcggt
    10681 gtcgttcctc ttttcggccg cgctcgttgg cctgttgaca ccgtcgctca cccaccacga
    10741 ggcgaccgcc gaaggggagg agccgcagcc acgcggccgc tacttcgacg agctattggc
    10801 ggggctgcgc ttcgtctggc gcgaccggct ggtgcgcgcg atcgtgctga cggtgatgat
    10861 caccaacttc ctcgacgcgc cgctgctgtc ggtcgtgttg ccggtgtacg tccaccaggt
    10921 ctacggctca tcggtcgact tgggtctgct cttcggcgcc ttcggcggcg cggcactggc
    10981 cggagcagtc ggctatgggc tgatcggccc gcggctcccc cgccggctga ccttcgccat
    11041 cgccttcgtg atcgtcggcc tgccgatgtg gctgctggcg ttccagccgc cgttcgtcgt
    11101 cgcgctgctg ggattcgtgg tcatcggcct cgcggccggc cccatcaacc cgatcatctc
    11161 gaccgtcatc ctggagcgcg tgccgcgctc gatgcgcgga cgcgtcaccg gcacgatcac
    11221 ctccggcgcg ttcgtcgcaa tgccggcggg catgctcatc gccggcttcg tcagccagct
    11281 atacggcgtc atcaccgcga tcgtgctgat cgcggctgcc tatgtggtgg taacgctgtc
    11341 aatcttcgtc aacccggcaa tgcgcgagat ggaccgtatt ccctccggtg ctcaggcgtc
    11401 ccgccccgcg cggtaggcga tgacctcgac cttctcgagc gtcatcaggc catcggccag
    11461 catctcatca agctctggca ggatggcccg caccttttcc tccgtatcga cgacctcgat
    11521 caggatgggc aggtcctgcg acaggctcag gatgcgcgtg gagtgcaggt gctgtttcgc
    11581 gccaaagccc tcgatgccac gcaacacggt cgcgccggcg aggccgcgct cgcgcagccg
    11641 ctcgacgatc gcctggtaga gcggccggcc gtgccactgg tcgctttcac ccaggtagat
    11701 gcgcactagc aggcccgttc cctctagctt catgagattg ccctcccgag cgccaggcca
    11761 ccgatcaccg caatcattcc gatcacgacc gacccgccga gattcaacgc ggccagcagc
    11821 cacgcaccgt cctcgatcat gcgccagctt tcgagcgtca gcgtcgagaa agtcgtgtac
    11881 gcgcccagga agccgatcat cagcggcccg cgcagctcgg ccggtagcgc cgcgcgttcg
    11941 gtgatcaacg caaacagcag gcccacgagg aacgagccgc tgacgttgac gatgagcgtg
    12001 ccccacggaa atgaggcgaa ccgctggctt acctccgcat caacgagata gcgcgctacc
    12061 gcgccggcga agcctccggc accgatcaac aggactccga ccacgggcaa cctcctgcgc
    12121 aacattcctg cgtaggagct atcagccaat ccggcggtta aggcgagccc catcgcctgc
    12181 gccccgcatg ctaatgggcc agctctccga ggcggcggtc gaggtagcgg cgatcaacgt
    12241 cgttagtggc gagttcgcgg gcgcgcgaaa gcgcggcgcg cgcgtcctcg agccggccca
    12301 cgcggcgcaa cagctcaccg cgcgtcgagt gcaacaggtg gtagccggcc agctcgccgc
    12361 tcgccgccat ggcgtcgacg agggcgagac ccacagcagg tccactggcc atcgagacgg
    12421 cgacggcacg gttcagggcg atcacggggg agggctgtgc ggcaacgagc tcgtcatata
    12481 gagaaacgat gcgcggccag tcggtgtccg cggcagctgc ggctcgcaca tgctccgccg
    12541 cgatcagtgc ctgcagcacg tacggcccgc gcggcccaag ccgctctgcc cggtccagca
    12601 ccgcgagacc ttctgtgatg cgcgcccggt cccaccgcga gcggtcctgg tcggccagca
    12661 acacgagatc gccctgcgca tcctggcggg cgtcacggcg cgagtcctgc agcaacaaca
    12721 gcgcgagcaa tcccagtgcc tccgattcac ccggcatcag cgccaccacg agtcgcgcca
    12781 gccggatcgc ttcagacgcc agctcgcgcc gaaccacggt cgcgcctgca gtcgccgagt
    12841 aaccctcgtt gaacacgaga tagaggacat gcagcactga cccgagccgc tccggcaacg
    12901 cctcggccgc aggcaccaca tacggaatcg ccgcatcgcg gatcttgcgt ttggcgcgca
    12961 ccaggcgctg ggcgagcgtc gcctctggca gcaggaaggc gcgcgcgatc tcgggcgtgc
    13021 gcaggccacc caagaggcgc aaggtcagtg cgatctgcgc gtcggcagcc aatgcggggt
    13081 ggcagcaggt gaagatcagt cggagtcggt cgtcctcgac tgctggtcgg tcggtcagca
    13141 tgtccggtcc cggctcctgg taagccccct cggtcgtcat cagtcgcgcg agctcctcgg
    13201 tcttctccgt gaatctcttc gcgcggcgca gccggtcgat cgcgcgattg cgcgcggtcg
    13261 tggcgatcca cgcaccgggg ttgggcggca ggccgtcgcg cggccagtgc tcgagcgcgg
    13321 caatgaacgc gtcctggacc gattcctcgg ccagatcaat gtcaccgact gcccggatca
    13381 gcccggcgac gacccggccc tgctcctcgc gaaagatccg atcgacggcg tcatacgccg
    13441 ccggccccgg ctcagccagc ggcgatcgtc tgcggcaccg ggttgccagc cgcgtccatg
    13501 ccatcgcccg ggttgcccgg cgacgggaag tcgaccaccg gccgcacttc gatcgtgccg
    13561 cgcaccgcgc ccgggatctg cgccgcccac ttgatcgcct cgtccaggtt ctcggcctcg
    13621 atcaagtaga agccgccgaa ctcctccttg gtttccgcga acggcccgtc ggtgacgagc
    13681 gtctggccgt cccgaacacg aacagaagtt gcggtcttgg cctcgtgcag cgcctcaccg
    13741 ccgcgcatga cgtcggccgc gacggccgca cgagtgaacc cgttgtaggc gttcatgacc
    13801 tcgacatcct gctcgggcgt cgactcccac ggctcaacgt agatcagcag cgcatagcgc
    13861 atgtcggcgt tcctcctggt tgactcgatg ttcgggcttc agtgcccctt cagcaagtca
    13921 acgattggcg caacgccaaa tcgacacaac gggcctatgc tgccgccatg ccgacttatc
    13981 tcgataccca ccgccacgtg ccgggcttga ccgcggcggc cgtcgccgat gcccacgcga
    14041 aggacatgga ggtcggcccg aagtacggcg tgacctacgt gcgctactgg ttcgacgagg
    14101 cgacaggccg cgtctactgc cttgtcgagg ccccgtcacc cgagctcgcc gagcaggttc
    14161 atcgcgaggc gcacggcctt ctggccgacg agatcgtcgc cgtcgtcgag ggcgcctgaa
    14221 cccctagcgg gagaggacgg cttcggcctc gcgtagcgcc tcgtccacgc ctgccccgct
    14281 gccggattgc cacgccgcgc cgaactcggc gcccagcacc ctctgcagcc gcgcgagctg
    14341 ctggtccatg gcggcctgac ccgcgggtga gagacgcgcg cccgtccggt cgcgcagcga
    14401 cgttgcggcg gccaggatcc gcgccgcgcg ggtagcatcg agatccatcg cgcccgcagc
    14461 gaaccgctcg agcgcgccac agatcccggg cgagtcgccc agccggcagc ggatctcgag
    14521 ggcgccatac agcagccgca gcgcctcggc ggccttgcct tcgtcagcgt ccacgtcggc
    14581 gaggagcgtc atcagccgcg cctcaccgcg ctggtcgccc agctggcgat agattgcaag
    14641 cgcctgctca tagcgttggc gcgctgctgc gtggtcgctc tgtcgggcgg ccgattgacc
    14701 gtagctgctc tgggcgtagg ccatgcccca aatgtcgccg cttgcggcca cgatctcgat
    14761 ggcctgctcg tagtagccac gagccgcctg ggcgtcgccc tggctgtccg ccacgtcggc
    14821 caggttgacg tagacgaagg ccctgttggg atcctccgtg cctgcgatgg cgatcgcgcg
    14881 ctcatagagc gtgcgtgcct gcccgaggtc accggtgtgc attgcggcgt tggccaggtt
    14941 gtgcaacgcg aattggacgc cctgtttgct gcccagctgc tcttgcactg caaggctctg
    15001 ctcgtggtac cgcaccgctg cggcgtggtc gccctgggcg gcggccagga tgccggccgc
    15061 ggtcagcacg tcagcgcgcg tcgtcgacga atcgccgggc gtggccgcga gcacgcgctc
    15121 gagccactgc cggccctcgc gcaggtagcc gcggatctcc cagaagcgcc acagcccgga
    15181 cgcgagctcg agcgccgcac tcgcgccgcc ggcttcgttg aggctccatg acagcgcggt
    15241 gcgcaggttg tcgtgctgcg cctcgagagc gtccagcgac tctgcggatt ccgggccgcg
    15301 aaagaaagct ggcgcagcgc gccggacgaa ctcgagatac cagtcgcgat ggcgctcgta
    15361 ggcgcccgcc gcctcgcctg cttcgagcga tcgctcccgg gcatagccgc gcaccgtttc
    15421 gagcaggcga tagcgatcct gctggcgatt gacaagcgat ttctcaacaa gccttgtgac
    15481 gtggtcgatg acctcttccg acgccacgtc tgtgcccacg cagacggcct cggcagcctc
    15541 cagtgcgaag gcaccggaga acaccgacag ccgccacagc accgagcgct ccgtttcggg
    15601 cagcaggtcg aagctccagt caatcgcggc gcggagagtc tggtggcgcg gcatgaccgt
    15661 gcggctccca ccggtgagca gccggaacct gtcgtcgagc cgctccgcta tctgctgggg
    15721 cgacagtgcg cgaacgcgcg cggcggcgag ttcgatggcc agcgggatgc cgtcgaggcg
    15781 ctcgcagatt tcaacgacag cgggggcgtt gtcgtcagtg atggtgaagc tcgggtcggc
    15841 cgcacgggca cgctcgcaga agaggtcgac tgcctcaaac tcggccgcga ctgcggcgga
    15901 cacggtcctg gcactcgtcg gcagagtcag cgaccgcact ggatacatcc gctcgcccga
    15961 cacgccgagc gcctcgcgac tcgtggcaat gatgcgcagg ccggggcagc cgcgcagcag
    16021 cgcgttggcc agtcgcgcac aggcctcgat cacgtgctcg cagttgtcga ggatgagcag
    16081 cgcctggcgg gtgcgcaggt gtcccagcac cgattcctgc gcgtcggcgt ccgacgcctc
    16141 ggcaatgccc agagcaacgg ccacctgctg ggcgacgagg ttctcgtcgg tcagcgtctc
    16201 gagctcgacg agccatgccc cgtcggcgaa cgtgtcgagg agctcggccg cgacctgtat
    16261 cgccagtcgc gtcttgccga cgccgcccgg cccggtcagc gtgacgagcg gcgcttctgt
    16321 cgtgagccgc cgcacctcgg caacgtcgcc ctggcgcccg acgaagctgt tcaggtggcg
    16381 cggcaggttg ttgggcagcg tcaccagcga cttgaggggc gggaatgcct ggggcaaacc
    16441 ctggatcgtc agctggtaca ggtgctcggg ctgagcgagg tccttcagcc agtgctcgcc
    16501 caggtcggac aagaccgcct cggccggcag gtcggtggcc accaggtcac gcgtcacctg
    16561 cgacagcagg acctggcccc cgtgtcccgc ggccgcgatg cgggccgcgc ggtgaacgtc
    16621 catgccgacg tagccactgc gccgagtgac cgcttcgccc gtatggatgc ccatgcgtgc
    16681 acgcacgctc gcgccaccgg gccagtcgcg ctccgccatc gcgagctgtg cctcgatcgc
    16741 cgccgaggct gcggagcgag agctcggaaa gctgtggaac aggccgtcgc cagcctggtc
    16801 gagctgcgtc ccgccatgct tggtgaagac cgcctcgatc aggtcgtggt atgcctcgag
    16861 cagcgggccg taagcatcgc gcag
//
LOCUS       DATBRX010000137         6383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3956266, whole genome shotgun sequence.
ACCESSION   DATBRX010000137 DATBRX010000000
VERSION     DATBRX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3956266"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VIF08_05275"
     CDS             <1..1245
                     /locus_tag="VIF08_05275"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEY8135434.1"
                     /translation="PVHQVDPVKVDPTSRWQVRAHSISECVEKLSEVWTSAAIQTEQP
                     DVTEDALERAKGDPRLARHMTDASSVRVRMRTSVLTLVVVAPRPETAERALAAINHLN
                     KRHPSRAIVVSPGDFDGPATTDAHIYAECRLSDNSSSEICTEQILVKTGGELAQHLSR
                     VVAPLLIHDLPVVLWWPDDPPFGSKQFTEIVDAADRLLVDSGTFHEDGGARLAGLATV
                     VAGGVAVTDIGWLRLSLWRELLAGLFDHPLLTRELDHIRSVRVDVARPTDTLRVSRAA
                     FYLGWLASRLGWETARPLSPRGGDSDELHGAFRHGKREIAVEIHPVRATLDGSKRAPG
                     SLVRVDIEAARPKSQVRARVTRQSDHLLATADWNGASVARRAGRLEPFDEAPFIAEAL
                     ERPGLDRTFEQALIRAVRLLGG"
     gene            1238..2062
                     /gene="pgl"
                     /locus_tag="VIF08_05280"
     CDS             1238..2062
                     /gene="pgl"
                     /locus_tag="VIF08_05280"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEY8135435.1"
                     /translation="MAESARPDGAYGGAEIVVVDDPAAVAREAARRTIAALAAAVSER
                     HVAHIALTGGSTAAPLYRELAAPANHAALDWSRVHLWWGDERFVPSDHPDSNAGLAYA
                     QLLGGGEESAASALPIDPGNVHPVPVEEALSDDDPVALAAQRYAEELTRHILLARGGV
                     PRFDVLLSGLGPDGHVLSIFPNSPALADDAPIALAIAAPDHVEPHVARITLSARSIAD
                     AGLVLVMATGAGKADIVASVLGSERDVNRWPAQSALSANGVWLLDQAAAAQLRSSR"
     gene            complement(2041..3312)
                     /gene="chrA"
                     /locus_tag="VIF08_05285"
     CDS             complement(2041..3312)
                     /gene="chrA"
                     /locus_tag="VIF08_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY8135436.1"
                     /translation="MSVTKVPLRAALRLWAYVGLNSFGGPAGQIAVMHREIVERRRWV
                     DEQRFLHALNYCMLLPGPEAQQLATYLGWWLNGLRGGLVAGLFFVLPGVVVLLLLSVL
                     YALYHETALLAALFYGVKPAVVALVAQAFLRLAGRTLKTNLARAIAAAAFVALFFFDV
                     PFPLIIVAAALVGLLATRRSPATGAGAEPVEQVARPPLARTFRTAAVFLVIWLAPVVA
                     CLALLGPGSIFSQLTLFFAFAALISFGGAYALLAFIAQQAVDIYGWLSARQMVDGLGL
                     AETTPGPLIIVVQFVGFIAAFAQPGGLEPVTAGVVGALLVSWVTFAPSFLWIFTGAPY
                     VEYLRSRSGLNAALAGVGPAVVGVILNLAAWFALQTFFRVPGELASIDLFAVGLAIAA
                     FVALVRLKWPLLLTLGLSAAVGAGYYLLLRS"
     gene            3386..4282
                     /locus_tag="VIF08_05290"
     CDS             3386..4282
                     /locus_tag="VIF08_05290"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY8135437.1"
                     /translation="MIGRLLLRILRAQYGWARPFGDFNVRWLRAVFRPIGPVKDFLHG
                     KWLGHSVHAVLTDLPLGVLSLTVLFDMLNLRQAADISMTVGVLGMVAAAVAGLADYSD
                     TDDDARAAGTVHGTLMVIALVVYLVSLWLRLANPAAADRTVEIVTQLIAYGIMLAGAY
                     VGGELVYTLGNMVNRHAWRFGTKSDWTRLDVTDIPENKPTAAKAGTQQLVVIRTGDTV
                     YALHAQCAHAGGPLPSGKIVDGCIECPWHFSRFDLATGRRTRGPTTFDQPRYEVRAAD
                     GGGWEVKRVLDPAGQNIAPVTG"
     gene            complement(4279..5235)
                     /locus_tag="VIF08_05295"
     CDS             complement(4279..5235)
                     /locus_tag="VIF08_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8135438.1"
                     /translation="MAGGALIGPGPIFGLLSALSWGAGDFSGGVISRYASTFTAVMTA
                     QVLGLAGTLLWLPFSAEAPLSGQAVMFAILAGAFGVSGLFCFYYALGRGTMGVIAPIS
                     ALIGAGGPVLLAIYNGEHVSAFRLAGIILALVAVVLISLPGGESTPAEQRRVRLDMRE
                     LPIVILSGLGFAGFFVFIGHATADGGVLWPLAVVRTVGVAMVLAGFTFLLLRTPAVPL
                     RQRASKLLGIPRLRSWPGSRLALIGVFVIAGLGDLGGNFFFVTAQHVDLFSVAVVLAS
                     LYPVVTTVLAVLLLRERLRGLQIAGITMATLSVVLLSNAFGV"
     gene            complement(5217..>6383)
                     /locus_tag="VIF08_05300"
     CDS             complement(5217..>6383)
                     /locus_tag="VIF08_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135439.1"
                     /translation="RLQNELREGDIARRLAGRTDLAERVRQAEQERVEGLTPLGMADL
                     AAAETALAVETDHMARIGSLRAEYRGLMGDAPLPDEDVGELRDKAAAEGEEARHALAG
                     MGDIGADPERFLNGFQLAITRLTSEREAAFTAEAQAEARVNANEIDAEKVAADAEALI
                     VAEEALAAAERRLRIYEEALATLNAAERGTMKKAARFLEGRMARDVERITNGRYRRLR
                     VDEATLTFSVHSPETDGWIDVRRLSQGTLDALYLCARLGIVRQVTAPAAPPLLLDDPF
                     VTFDDERATRGLALLKDMARDLQVILITTSDRYDSLADNVVVLPAPTERDDPEPIAPT
                     PSAESMSVWSSTALPPAEPRPTSSRPPVAVNSGAANGNAPTPPVEPAPLWPEEH"
BASE COUNT         1049 a         2246 c         2215 g          873 t
ORIGIN      
        1 cccgttcacc aggtcgaccc ggtcaaggtc gatccgacca gccgctggca ggtgcgcgcg
       61 cacagcatca gcgaatgcgt cgagaagctg tccgaggtgt ggacgtcggc ggcaattcag
      121 acggagcagc ccgacgtgac ggaggacgcc ctggagcgcg ccaagggcga tccgcggctc
      181 gctcggcaca tgaccgacgc gtcttcggtg cgtgtccgga tgcgcaccag cgtgttgacg
      241 ctggtcgtcg tcgcgccgcg gccggagacg gccgaacgcg ccctggcggc aatcaaccat
      301 ctcaacaagc gccacccctc gcgcgccatc gtggtctcgc ccggcgactt cgacggcccc
      361 gcaacgaccg atgcgcacat ctacgccgag tgccgcctgt ccgataactc gagctcggag
      421 atctgcaccg agcagatcct ggtcaagacg ggcggcgagc tggcgcaaca cctgtcgcgc
      481 gtcgtcgcgc cgctgctgat tcacgacctg ccggttgtcc tgtggtggcc cgacgatccg
      541 ccgttcggtt cgaagcagtt cacggagatc gtcgatgccg cggaccggct gcttgtcgac
      601 tcgggcacgt tccacgagga cggcggcgcg cgactcgccg ggctggcgac cgtggtcgcg
      661 ggcggggtgg ccgtgaccga catcggctgg ctgcgcctgt cgctatggcg cgagctgctc
      721 gccggcctgt tcgaccatcc actgctgacg cgcgagctcg atcacatcag gagcgtccgc
      781 gtcgacgtcg cgcggccaac agacacgctg cgcgtctcgc gtgcggcctt ctacctgggc
      841 tggctcgcgt cacgcctggg ctgggaaacc gcgcgaccgc tatcgccgcg cggcggtgat
      901 tccgacgagc tgcacggcgc gttccgccac ggcaagcgcg agatcgccgt cgagatccac
      961 ccggtacggg caacgctcga cggcagcaag cgcgcgccgg gctcgctcgt tcgcgtcgac
     1021 atcgaggccg cgcggcccaa gtcgcaggtg cgcgcgcgcg tcactcgcca gtccgaccac
     1081 ctgctcgcga ccgccgactg gaacggcgcg tcggtcgctc gccgggcggg gcgcctcgaa
     1141 cccttcgacg aggcgccgtt catcgccgag gcgctcgagc ggccgggcct cgaccggacg
     1201 ttcgagcagg cgctcatccg ggccgtgagg ctgctcggtg gctgagtcgg cgcgcccaga
     1261 cggcgcctac gggggcgcgg agatcgtcgt cgtggacgac ccggcggcgg tcgcgcgcga
     1321 ggccgcacga aggaccatcg ccgcgctggc cgcggcggtc agtgagcgcc acgtggccca
     1381 catcgcgctg accggtggct cgacggccgc accgctctac cgcgagctcg ccgcgcccgc
     1441 caatcacgcc gcactcgatt ggtcgcgcgt ccacctgtgg tggggcgacg aacgcttcgt
     1501 gcccagcgac catcccgatt ccaacgccgg gttggcatat gcgcagctgc tgggcggcgg
     1561 cgaggagagc gccgcgtccg cattgccgat cgaccccggc aacgtccatc ccgtgccggt
     1621 cgaggaggcg ctgagcgatg acgatcccgt cgccctcgcg gcgcagcgct acgccgagga
     1681 gctgacgcgc cacatcctgc tggcgcgcgg cggcgtgccg cgcttcgacg tgctcctgag
     1741 cggccttggc ccggacgggc atgtgctgtc gatcttcccg aacagtccgg ccctggccga
     1801 cgacgcaccc atcgcgctgg ccatcgccgc gcccgaccac gtggagccgc acgtcgcgcg
     1861 catcaccctg agcgcgagat caatcgccga cgcgggtctg gtcctggtga tggcgaccgg
     1921 cgccggtaag gcggacatcg tcgccagcgt gctcggcagc gagcgcgatg tgaatcggtg
     1981 gccggcccaa tcggccctct cagctaacgg cgtctggcta ctcgatcagg cagcagcggc
     2041 tcagctgcgc agcagcaggt aatagcccgc gccgactgcg gcgctgagcc ccagggtcag
     2101 cagcagcggc cacttaagcc gcaccagcgc gacgaacgcg gcgatcgcga ggccgaccgc
     2161 gaagaggtcg atcgacgcca gctcgccggg cacccgaaag aacgtctgca gcgcgaacca
     2221 cgcagcgagg ttgaggatga cgcccacgac cgccggccca acgcccgcca gcgcggcgtt
     2281 caggccgctg cgcgaacgca ggtactccac atatggcgcg ccggtgaaga tccacaggaa
     2341 gctcggcgcg aaggtgaccc acgagacgag cagggcaccg accaccccgg ccgtcaccgg
     2401 ttccaggcca ccgggctggg cgaacgccgc gatgaagccc acgaactgga cgacgatgat
     2461 cagcggcccg ggcgtcgtct cggccaggcc caggccatcg accatctggc gcgcggacaa
     2521 ccagccgtag atgtcgaccg cctgctgcgc gatgaaggcc agcagcgcgt atgcgccgcc
     2581 gaacgagatc agcgccgcga acgcgaagaa gagcgtcagc tggctgaaga tgctgcccgg
     2641 cccgagcagt gcaaggcacg cgaccacggg tgccagccag atgaccagga agaccgccgc
     2701 ggtccggaaa gtgcgggcga gcggcggccg cgcgacctgc tcgaccggct ccgcgcccgc
     2761 gcccgtcgcc ggtgagcgtc gcgtcgctag cagcccgacc agcgctgccg cgacgatgat
     2821 gagcgggaac ggcacgtcga agaagaacag cgccacgaat gcggccgcgg cgatggcgcg
     2881 ggcgaggttg gtcttgagcg ttcgcccggc aagacgcagg aacgcctgcg cgacgagcgc
     2941 cacgaccgcc ggtttgacgc cgtagaagag agccgcgaga agcgcagtct cgtggtagag
     3001 cgcgtacagc acggaaagaa ggagcagcac gaccacgccc ggcaggacga agaaaagtcc
     3061 cgcgaccaaa ccgccgcgca acccgttgag ccaccaaccg aggtacgtgg cgagctgctg
     3121 cgcctctggc ccgggcagca gcatgcagta gttcagggca tgcaggaagc gctgctcatc
     3181 gacccaccgg cggcgctcga cgatctcgcg gtgcatgacc gcgatctgcc cggcgggtcc
     3241 gccaaagctg ttcaggccga cgtaagccca cagccgcagc gccgcacgaa gcggcacttt
     3301 tgtcacgctc attcaaccgg cagcgtagtc gagcgcatct gatgggaggg ctcgggacgt
     3361 agattgtccg tagggaggtg cccgcgtgat tggtcggctg ctgctccgca tcctgcgcgc
     3421 ccagtacggt tgggcgcggc ccttcggtga cttcaacgtg cgctggttgc gcgccgtctt
     3481 ccgaccaatc gggccggtca aggatttcct gcatggcaaa tggctcggcc actccgtcca
     3541 cgcggtcctg acggacctgc ccctcggtgt gctttcgctc accgtcctgt tcgacatgct
     3601 gaacctgcgc caggcggcag acatctcgat gacggtcggc gtcctgggca tggtcgcggc
     3661 cgccgtggcc ggcctggccg actacagcga caccgacgac gacgcgcgcg cggccggcac
     3721 cgttcacgga acgctgatgg tcatcgccct ggtcgtctat ctcgtgtcgc tatggctgcg
     3781 cctcgccaac cccgcggccg cggacaggac cgtcgagatc gtcacccagc tgatcgccta
     3841 cgggatcatg ctcgccggcg catacgtggg tggcgagctc gtttacacgc tgggcaacat
     3901 ggtcaatcgc cacgcctggc gcttcggcac caagagcgac tggaccaggc tcgacgtgac
     3961 ggacataccg gagaacaagc ccacagccgc caaggcgggc acgcagcagc tggtcgtgat
     4021 caggaccggc gacacggtct atgcgctgca cgcccagtgc gcccacgccg gtggcccgct
     4081 gccgagcggc aagatcgtgg acggttgcat cgaatgtccg tggcatttct cacggttcga
     4141 cctcgccacg ggccggcgga cgcgcggtcc cacgacattt gatcagccgc gctacgaggt
     4201 tcgcgcggcg gacggtggcg gctgggaagt aaagcgcgtg ctcgacccag ccggccagaa
     4261 catcgcgcct gttacgggct agaccccgaa cgcgttcgac agcaggacga cgctgagtgt
     4321 cgccatggtg atgcccgcga tctgcaggcc gcgcagccgc tcgcgcagca ggagtaccgc
     4381 gaggaccgtg gtcaccaccg ggtagagcga cgccagcacg accgcgacac tgaacaggtc
     4441 gacgtgctgg gccgtgacga agaagaagtt gccgcccagg tcgcccaggc cggcgattac
     4501 gaagacaccg atcaacgcga gccggctgcc cggccagctg cgcaggcgcg gtatgcccaa
     4561 cagcttcgag gcgcgttggc gcaggggcac ggcgggcgtg cgcagcagca ggaaggtgaa
     4621 gcccgcgaga accatcgcga cacccaccgt gcgcaccacc gcgagcggcc acagcacgcc
     4681 gccgtcggcg gtcgcatggc cgatgaacac gaagaagccg gcgaatccaa ggccggacag
     4741 gatgacgatg ggcagctcgc gcatgtcgag ccgaacccgg cgctgttctg ccggagtcga
     4801 ctcgccgccg ggcagtgaga tcagcaccac cgcgacgagg gcgaggatga tgccggccag
     4861 tcggaaggcc gacacatgct cgccgttgta gatcgccagc agcactggcc cgcctgcgcc
     4921 gatcagtgcc gagattggcg cgatgacacc catcgtgccc cggcccagcg cgtagtagaa
     4981 gcagaacagg ccggacacgc cgaatgcgcc ggcgagtatg gcgaacatca cggcttggcc
     5041 gctcagcggc gcctccgcgc tgaacggcag ccagagcaac gtgccggcga ggccaagcac
     5101 ctgtgcggtc atgaccgcgg tgaaggtgct cgcatagcgg ctgatgacgc cgccactgaa
     5161 atcgcccgct ccccaggaga gcgcggacag cagcccgaag atcgggcctg gaccgatcag
     5221 tgctcctccg gccagagcgg cgccggctcg accggcggcg ttggggcgtt gccgttagcc
     5281 gcgccgctat tgacagcgac cggcgggcgc gaggatgttg gccgcggctc ggccggcggc
     5341 agggcggtgc tcgaccagac cgacatcgac tcggccgatg gcgtgggagc aatcggctcc
     5401 gggtcgtctc gctccgtcgg cgcaggcagg acgacgacgt tgtcggccag tgagtcgtat
     5461 cggtcggagg tggtgatcag gatgacctgc aggtcgcgcg ccatgtcctt gagcagcgcc
     5521 aggccgcgcg tcgcgcgctc gtcgtcgaag gtcacgaatg gatcatcgag caagagcggc
     5581 ggggccgcag gcgcggtcac ctggcgcacg atgcccaggc gagcacacag atagagcgcg
     5641 tcgagcgttc cctgggacag ccgccggaca tctatccagc cgtcggtctc aggtgagtgg
     5701 acgctgaacg tgagcgtggc ctcgtcgaca cgcaggcggc ggtagcggcc attggtgatg
     5761 cgctccacat cgcgcgccat gcggccctcg aggaagcgcg ccgctttctt catcgtgccc
     5821 cgctcggccg cgttgagcgt cgccagcgct tcctcgtaga tacgcaaccg tcgctcggcc
     5881 gcggccagcg cctcctcggc gacgatgagc gcctctgcgt cggccgcgac cttctcggcg
     5941 tcgatctcat tggcgttgac gcgcgcctcg gcctgcgcct cagcggtgaa cgcggcctcg
     6001 cgctccgagg ttagccgcgt gatcgccagt tgaaagccat tcaggaagcg ctccgggtcg
     6061 gcgccgatgt cgcccatccc ggcaagcgcg tggcgcgcct cctcgccttc cgcggcggcc
     6121 ttgtcgcgca gctcgcccac gtcctcgtct ggaagggggg cgtcgcccat cagaccgcgg
     6181 tactcggcgc ggagcgaccc gatcctggcc atgtgatctg tctcaacggc gagcgccgtc
     6241 tccgcagccg cgaggtccgc catgcccagc ggtgtcaggc cctcgacgcg ctcctgctca
     6301 gcctggcgga cgcgctccgc gagatccgtc cgacccgcca ggcggcgcgc gatgtctccc
     6361 tcgcgcaact cgttctgcag ccg
//
LOCUS       DATBRX010000138         5761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3734289, whole genome shotgun sequence.
ACCESSION   DATBRX010000138 DATBRX010000000
VERSION     DATBRX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3734289"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VIF08_05305"
     CDS             complement(<1..371)
                     /locus_tag="VIF08_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8135440.1"
                     /translation="MSSQPRPLVLGIFRRGPLMLVGEGYDRVRDQRFYRPLGGQIEAG
                     ESPEQALIREMREELGDEIRPIRHLGELDNRFVFEGRPGWEQVHVLEAAFVSSAAPHV
                     NSVEAEGWNLKWIDPNALDLPL"
     gene            736..1419
                     /locus_tag="VIF08_05310"
     CDS             736..1419
                     /locus_tag="VIF08_05310"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135441.1"
                     /translation="MEVDTPFTSTVDCGPGTCQIPLDILAPEAGASLPTFVLIPGGPT
                     TFADRRYMDLISTGLAHRGAVVFLATYRSHAIGADDDATPFDIQCAIQFARAHTTDYG
                     GDPERLVLVGHSFASPLVIRAGANAELSTPDCLADADGVPDAILALSLFDPATPLGTP
                     NGIPFRLACGSLDTCSAGGAAMAEQLQDAGFDAEYLEFTGVDHFGMVDPGTPGLVDMY
                     FQVAELAGQ"
     gene            1465..2061
                     /locus_tag="VIF08_05315"
     CDS             1465..2061
                     /locus_tag="VIF08_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY8135442.1"
                     /translation="MITRNSPARTNGAARAAARAATEAPRRPRSIFPGTGAVHFSTRG
                     EYGTRLMVELARHHGTGPISLAEMADHESLPRPYLEQLVVSLREANLVTSTRGAHGGY
                     ELARAPQDIKMGEVLRALEGPIAPMVCASEDPSHAGICERMGFCNVNHLWLTVRSAIS
                     TALDSITLADLAVPRARHPFHSAPIALTASQGAQLTES"
     gene            2058..2966
                     /gene="sufC"
                     /locus_tag="VIF08_05320"
     CDS             2058..2966
                     /gene="sufC"
                     /locus_tag="VIF08_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY8135443.1"
                     /translation="MTQTLNPAQAAATIQDDRALVIKDLHVVPIADRSHEILRGINLT
                     VRQGEVHAIMGRNGSGKTTLAYALMGHPAYQVTAGEVLWKGHNLLKLSADKRSRLGIF
                     LAFQYPTAIPGLSVASFLRTALNAKSRPLDQAPSDIDPSDATRGGIKMADYRKLLREK
                     MALLKLDDSFAARYVNEGFSGGEKKRLEMLQMAVLAPHMAILDETDSGLDIDALRIVA
                     EGVNAQLNPDLGVLLITHYQRLLNYITPDQVHVLAAGQIVASGGRDLALRLEEEGYAP
                     ILREAGLEPDTDEEHEPAEPVEAASH"
     gene            2966..4213
                     /locus_tag="VIF08_05325"
     CDS             2966..4213
                     /locus_tag="VIF08_05325"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEY8135444.1"
                     /translation="MAIVRALDPVALRADFEIFKREFRGKRLAYLDSAATSLKPRVVT
                     DAVAGYLNNYSANVHRGIYEIGEEATAAYEAARQKIAAFIGARDAREIVMVRNATEAI
                     NLVAYAWGRKNINKADTIVTTEMEHHSNIIPWQLLTQEKDADLEFVSFDEDGRLNQDS
                     YDVLLRTKPKLVTFVAVSNGIGTINPVIDMVRRAHDAGALVLVDGAQAVPHQPVNVQE
                     LGCDFFVFSGHKSLGPPGSGALWARREILEAMPPFMGGGEMIREVHLRHTTFNDVPYK
                     FEAGTPDVSAAIGLGAALDYLSGLGMEHVRAHERELVEYALEVLPERVKSIRIHGTKS
                     ASERAGVITFNLADAHPHDVATLLDREAIAIRAGHHCTMPLHQRLGEEASARASFYVY
                     TGRDDIDRLAEALIKVERLFARN"
     gene            4239..4637
                     /locus_tag="VIF08_05330"
     CDS             4239..4637
                     /locus_tag="VIF08_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY8135445.1"
                     /translation="MDDLYRDEILEHYRNPHNFGTLETPTTVKEGANPLCGDRITLML
                     EIDDAGNVEDVAFTGRGCAISQASASMLTDEIKGKPLSDIAAMGRQQVLDNLGIEISP
                     ARMKCAMLSLETLRSAVSDRVAWQGKTDEE"
     gene            4627..5703
                     /gene="moeB"
                     /locus_tag="VIF08_05335"
     CDS             4627..5703
                     /gene="moeB"
                     /locus_tag="VIF08_05335"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEY8135446.1"
                     /translation="MRNNPVREVLPAEAVQLIDAGAVLLDTRETNEWNAGHLAGATLL
                     RPADVISKVASLAPDPSKTVVLYCRSGARSLTAAFQMAQLGYHDVVSMAGGIIRWRQE
                     GRPIEVPSAAPTTNSANRYARHLVMPEIGPAGQEKLGQAKVLVVGAGGLGSPALLYLA
                     AAGVGTLGIVDFDTVDASNLQRQVIHADDRVGMPKTESAARTLKGLNPDVRVVVSNEM
                     LTDANVDDVISGFDLVIDGTDTFETRYTLNDAAVRAHIPVVHASVFRFEGQLTVFKPF
                     DGPCYRCLYPTPPPPELAPGCSVAGVLGVVPGVMGLLQATEALKLLLGIGDPLVGRLL
                     LWDALDGTFDEVTLRRDPNCPACG"
BASE COUNT         1003 a         2014 c         1812 g          932 t
ORIGIN      
        1 agcgggaggt cgagcgcgtt cggatcgatc cacttcaggt tccagccctc cgcctcgacg
       61 ctgttcacgt gtggtgccgc ggaagagacg aacgccgcct ccagcacatg cacctgctcc
      121 cagccgggcc ggccctcgaa cacgaatcgg ttgtcgagct cacccaagtg acgaatgggg
      181 cggatctcgt cgccgagctc ttcgcgcatc tcgcgaatta gggcctgttc ggggctctct
      241 ccagcctcga tctggccacc gagcggccgg tagaaccgct gatcccggac gcggtcgtat
      301 ccctcgccga cgagcatgag cggcccgcgc cggaagatgc cgaggaccaa cgggcgcggc
      361 tggctgctca tttcgcccgg attgtcgtcg agtcacgctg gcacggctgg ccagaacggg
      421 tcgaggcccc tacctttgca ggctaaccag ggacggaacg cgcggtagga gcgaaaaccc
      481 atgaagatcc ggcgtcgtcc tcacctgcag cgattagcgc cggtcctatg gttctcgact
      541 ctggcgctgc tctcggcctg tggatcaggg gcagttgctt cgcccagcgc cactcccaag
      601 ccgcaagcga cagtctctcc ttccccggtc gccactgcgc cagtctctcc ttccccggtg
      661 gccagtgtga ccgccaggcc ctactcgccc cccacggctg gcgacttccc gccagagctc
      721 gtcgaggggc ttcgagtgga agtcgacacc ccctttacga gcacggttga ctgtggcccg
      781 ggcacgtgtc agataccgct cgacatcctc gcccccgaag ccggtgcatc gctgccgaca
      841 ttcgtgctca ttcctggtgg tccgaccaca ttcgccgatc gacgctacat ggatctcatt
      901 tcgaccggcc tcgcgcatcg cggagcggtg gtattcctag ccacgtaccg cagtcacgcg
      961 atcggtgcgg acgatgacgc gacaccgttc gatattcagt gcgcgatcca gttcgcgaga
     1021 gctcacacca ctgactacgg aggtgatccc gagcgcctgg ttctcgtggg ccattcattt
     1081 gccagtcccc tcgtcatccg ggccggcgcg aacgcggagc tgtcaacacc cgactgcctg
     1141 gccgacgcgg acggtgttcc ggatgcaatc ctcgccctca gcctgttcga cccggccaca
     1201 cccctgggta caccaaatgg cattccattt cgtcttgcct gtgggtcgct tgacacatgt
     1261 tcagccgggg gcgcggcgat ggccgaacaa cttcaagacg ctggcttcga cgccgagtac
     1321 ctcgagttca ccggcgtaga tcacttcgga atggtggatc cgggcacgcc agggttggtg
     1381 gacatgtact ttcaggtcgc tgagctcgcc ggtcagtagc tgcgattgcg ggcctatcgg
     1441 gcctggtcta gactcaatcc tgacgtgata actcggaatt caccggctcg gacgaacggc
     1501 gctgcgcgcg cagcggctcg cgcggctacg gaagcgccac gccgcccacg gagcatcttc
     1561 cccggaaccg gcgcggtcca tttctcgacg cgcggcgaat acgggacgcg cctcatggtc
     1621 gagctggcac gccaccacgg cacgggtcca atctcgcttg ccgagatggc tgaccacgaa
     1681 tccctgccgc ggccgtatct agagcagctc gtcgtcagcc tgcgtgaggc caacctcgtc
     1741 accagcaccc gcggcgccca tggcggctac gagctggcga gagcgcccca ggacatcaag
     1801 atgggcgagg tgctgcgcgc cctcgaaggg cccatcgcgc cgatggtttg cgcgagcgag
     1861 gatccgtcgc acgccgggat ctgcgagcgc atgggcttct gcaacgtcaa ccacctgtgg
     1921 ctaacggtcc gctcggcgat cagcaccgcg ctcgactcga tcaccctcgc cgacctggcc
     1981 gtgcctcgcg cgcgccaccc attccactcc gcgcccatcg cgctgaccgc gagccagggc
     2041 gcccaactga cggagtcatg acccagaccc tcaacccagc ccaggccgcc gccaccattc
     2101 aggacgaccg cgccctcgtc atcaaggact tgcacgtcgt ccctatcgcc gaccggagcc
     2161 acgagatcct gcgcggcatc aacctgaccg tccgccaggg cgaagtgcac gcaatcatgg
     2221 gccgcaacgg gtcgggtaag acgacgctcg cctacgcgct gatgggccac ccggcgtacc
     2281 aagttaccgc gggcgaggtc ctgtggaagg gccacaacct gctgaagctc agcgccgaca
     2341 agcgcagccg cctgggaatc ttcctggcct tccagtaccc caccgccatc ccgggcttga
     2401 gtgtcgcgag cttcctgcgt accgctctca atgccaagag ccgcccgctc gatcaggcgc
     2461 cgtcggacat cgacccatcg gacgccaccc gcggcggcat caagatggcc gactatcgga
     2521 aactcctgcg cgagaagatg gcgctgctca agctcgacga ctcgttcgcg gcgcgctacg
     2581 tcaacgaggg tttttcaggc ggtgagaaga agcgcctcga gatgctgcag atggccgtcc
     2641 tcgcgccgca catggcgatc ctcgatgaaa ccgacagcgg cctcgacatc gacgcgctgc
     2701 gcatcgtggc cgagggcgtc aacgcccagc tcaaccccga tttgggcgtc ctgctgatca
     2761 cccattacca gcgcctgctc aactacatca cgcccgacca ggtgcacgtg cttgccgccg
     2821 ggcagatcgt cgcttcaggt ggacgggacc tcgcgctgcg cctggaagag gagggctatg
     2881 cgcccatcct gcgcgaagcc ggcctcgagc ccgacaccga cgaagagcac gagcccgcgg
     2941 aacctgtgga ggccgcgagc cactgatggc gatcgtccgc gccctcgacc cggtcgcgct
     3001 ccgcgccgac ttcgagatct tcaagcgcga gttccgcggg aagcggctcg cctacctcga
     3061 ctcggccgcg accagcctga agccgcgcgt cgttaccgac gcggtcgcgg gctacctcaa
     3121 caactacagc gcgaacgtcc atcgcggcat ctacgagatc ggcgaggaag cgaccgctgc
     3181 ctacgaggcg gctcgtcaga agatcgcggc gttcatcggc gccagggacg cgcgtgagat
     3241 cgtcatggtg cgcaacgcga cagaggcgat caacctcgtc gcctacgcgt ggggccgcaa
     3301 gaacatcaac aaggccgaca ccatcgtcac gacggagatg gagcaccact cgaacatcat
     3361 cccttggcag ctgctgaccc aggagaagga cgccgatctc gagttcgtct ccttcgacga
     3421 ggacggccga ctcaaccagg actcctacga cgtcctgctg cgcaccaagc ccaagctggt
     3481 cacgttcgtt gccgtatcga acggcatcgg cacgatcaac ccggtcatcg acatggtcag
     3541 gcgggcccat gacgccggcg ccctggtgct ggtcgacggc gcgcaggccg tgccccatca
     3601 gccggtcaac gtccaggagc tgggctgcga cttcttcgtg ttcagcggtc acaagtcgct
     3661 cggcccgccc ggcagtggcg cgctctgggc gcggcgcgag atcctcgagg ccatgccgcc
     3721 cttcatgggt ggcggcgaga tgatccgcga agtccacttg cgccacacga ccttcaacga
     3781 cgtgccctac aagttcgaag ccggcacacc tgacgtgagc gccgccatcg ggctgggtgc
     3841 cgcgctcgac tacctgtccg gcctgggcat ggagcacgtg cgcgcacacg agcgcgagct
     3901 cgtcgagtac gccctcgagg tgctgccaga gcgggtgaag agcatccgca tccacggcac
     3961 caagtcggcg agcgagcgcg ccggggtcat cacgttcaac ctcgcggatg cgcatccgca
     4021 cgatgtcgcg acgctgctcg accgcgaggc gatcgccatc cgtgccggcc atcactgcac
     4081 gatgccgctc caccagcgac tgggcgagga ggcgagtgcc cgcgccagct tctacgtcta
     4141 caccggtcgg gacgacatcg accgcctggc cgaagcgctc atcaaggtcg agcggctttt
     4201 cgcgcgaaat tagcgccgca gcgaaactag ccccgcacat ggacgacctg taccgcgacg
     4261 agatcctcga gcactaccgc aacccgcaca atttcggcac gctcgagacg ccgacgaccg
     4321 tcaaagaagg cgcgaatccc ctgtgcggcg accggatcac gctgatgctc gagatcgatg
     4381 acgccgggaa cgtcgaggac gtcgcgttca ccggccgcgg ctgcgcgatc agccaggcgt
     4441 cggcgagcat gctgaccgac gagatcaagg gcaagccgct gagcgacatc gccgccatgg
     4501 gtcgccagca ggtgctcgac aacctgggca tcgagatcag cccggctcgg atgaagtgcg
     4561 ccatgctctc gctcgagacg ttgaggtcgg ccgtctccga ccgcgtcgcg tggcagggca
     4621 agacggatga ggaataaccc ggtccgagag gttctgccgg ctgaggccgt gcaactgata
     4681 gacgcgggcg ccgtcctgct cgacacacgc gagacgaacg agtggaacgc agggcacctc
     4741 gccggggcga cgctgctgcg gccggccgac gtcatttcca aggtcgcgtc cctggcgcct
     4801 gacccgtcca agaccgtggt cctctattgc cgcagcggcg cacgatcttt gaccgccgcc
     4861 ttccaaatgg ctcagttggg ctaccacgat gtcgtatcga tggcgggcgg catcatccgc
     4921 tggcgccagg aaggccggcc gatcgaggtt ccctccgcgg ccccgaccac caactccgca
     4981 aaccgctacg cccgtcatct cgtaatgccg gagatcggtc ctgccggcca ggaaaagctg
     5041 gggcaggcga aggtattggt ggtcggtgcc ggtggtctcg gctcgccggc tttgctctat
     5101 ctggccgcgg ccggagtcgg gacgctgggc atcgtggatt tcgacacggt tgatgcgtcc
     5161 aatctccagc gccaggtcat ccacgccgac gaccgcgtgg gcatgcccaa gacggagtcc
     5221 gcggcacgga ccttgaaagg cctgaacccc gatgtgcgcg tcgtcgtgag caacgagatg
     5281 ctgaccgacg ccaatgtcga cgacgtgatc tctggcttcg acctggtcat tgatggcacg
     5341 gacacgttcg agacgcgtta caccctgaac gacgcggccg tccgcgccca catccccgtg
     5401 gtccacgcgt cggtcttccg gttcgagggc cagctgacgg tgttcaagcc gttcgacggg
     5461 ccgtgctacc gctgcctcta ccccactccg ccgccgccgg agctcgcgcc gggttgctcc
     5521 gtcgccggtg tgctgggcgt cgtgcccggg gtgatgggcc tgctccaagc gacggaggct
     5581 ctgaagctgt tgctgggaat cggagaccca ttggttgggc ggctgctcct gtgggacgcg
     5641 ctcgacggca cattcgacga ggtgacgctg cgtcgcgacc cgaactgccc ggcatgcggc
     5701 tgaccgcggc actcgtccag gagatcgtcg acgccgcgcg tgcggcgcta cccaatgaag
     5761 c
//
LOCUS       DATBRX010000139         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_607210, whole genome shotgun sequence.
ACCESSION   DATBRX010000139 DATBRX010000000
VERSION     DATBRX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607210"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VIF08_05340"
     CDS             <1..623
                     /locus_tag="VIF08_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY8135447.1"
                     /translation="PASTLVVNGRFGAGVYGIQLPGKHNAANGLMVLAAANVLGVLGE
                     EPGDGPAIGGFRGVGRRLELKGDVSGVTVLDDYAHHPTAIAKTIQAVRERYPGRRLWA
                     VYEPLTFHRTAAMLEGFADVLATADQVAIADIWAGRDPDTTITSAAALADAVTGRGGP
                     HATAPGSVEETADYLATQVRPNDVVLVMGGGRSYVIAERLVELLAK"
     gene            634..1065
                     /locus_tag="VIF08_05345"
     CDS             634..1065
                     /locus_tag="VIF08_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8135448.1"
                     /translation="MAAMAPLTPGDAQDLLALWKRGVERRSPDELLELFDPDIDHRPD
                     PFSESTIGLNGVHARLNAFAASQANVEFDAERIFVSGNTVLASWHGAYTRRATAERMR
                     VRGFMTLELSGERKIRRFRQWVAERAVGTDRTFDAERPEEG"
     gene            1065..1565
                     /locus_tag="VIF08_05350"
     CDS             1065..1565
                     /locus_tag="VIF08_05350"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY8135449.1"
                     /translation="MASGDVSFDVVSEFDQQELVNALDQARREITTRYDFKGAKAELE
                     LGKDEIILRADTDFRAKQMRDLLETKALRRALSLKIFDWGAIEEAGGNTVRQRIGLRR
                     GLPDDLAKKITKLIRDDFPKLKSQIQGDAVRVSGKSKDELQRLIARLRQESEGYPVPL
                     QFQNYR"
     gene            1704..>2071
                     /locus_tag="VIF08_05355"
     CDS             1704..>2071
                     /locus_tag="VIF08_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY8135450.1"
                     /translation="MADAIAAAGVRWAFTVPGESFLGLLDALPAAGIRVVATRHEGGA
                     AFMAEAVGQLTGQPAAVLGTRTVGASNMAIGIHTAYSNSTPLVALLGDVQRSHKGREA
                     FQESDLVARFGGLAKWAAQIV"
BASE COUNT          336 a          672 c          738 g          325 t
ORIGIN      
        1 cgccagcgtc cacgctggtc gtgaacggtc gattcggggc cggggtctat ggcattcaac
       61 tcccgggcaa gcacaacgcg gcgaacggcc tcatggtcct cgccgccgcg aacgttctgg
      121 gggttttggg cgaggagcca ggcgatgggc ctgccattgg cgggttccgc ggtgttggcc
      181 ggcggctcga gctcaagggc gacgtgagcg gcgtcacggt gctcgacgac tacgctcacc
      241 atcccacggc catcgccaag accatccagg cagtgcgcga gcgctatccg gggagacggc
      301 tctgggcggt ctacgagccg ctcaccttcc atcgcaccgc ggcgatgctc gagggcttcg
      361 ccgatgtcct ggccacggcc gaccaggttg cgattgccga tatctgggcg ggccgcgacc
      421 cggacaccac gatcacctcc gccgcggccc ttgcggacgc ggtcacgggc cgcggcgggc
      481 cgcatgcgac cgccccgggg agcgtcgagg agaccgcgga ctacctcgca acgcaggtca
      541 ggccgaacga tgtcgtgctg gtcatgggcg gcggccgctc gtacgtcatc gcggagcggc
      601 tggtcgagct gctcgccaaa tagactggca gtgatggcag cgatggcacc tttgacaccg
      661 ggcgacgcac aggatctgct ggcgctctgg aagcgcggcg ttgagcgccg ctcacctgac
      721 gagttgcttg agctgttcga tcccgacatc gaccaccgcc ccgatccgtt cagcgagtcg
      781 acgatcgggc tgaacggcgt ccatgctcgg ttgaacgcct tcgccgccag ccaggcgaac
      841 gtcgagttcg atgcggagcg catctttgtg agcggcaaca cggtgctggc aagctggcac
      901 ggcgcctata cgcgccgcgc gacggctgag cgcatgcgcg tgcgtggctt catgaccctg
      961 gagctgagtg gcgagcgcaa gattcggcgc ttccggcagt gggtggccga gcgagcagtc
     1021 ggtactgatc ggacgttcga tgctgaaaga ccggaggagg gctgatggcc agcggggatg
     1081 tttcatttga cgtggtcagc gagttcgacc agcaggagct cgtgaacgcg ctcgaccagg
     1141 cgcggcgcga gatcacgacg cgctatgact tcaagggcgc caaggcggag ctggagctgg
     1201 gcaaggacga gatcatcctg cgcgctgaca ccgacttcag ggccaaacag atgcgcgacc
     1261 tgctcgagac gaaggccctg cgacgcgccc tgtcgctcaa gatcttcgac tggggcgcga
     1321 tcgaggaggc tggcggcaac acggtgcgcc agcggattgg tctgcgccgc gggctgcccg
     1381 acgatctggc caagaagatc accaagctca ttcgcgacga cttccccaag ctcaagtcgc
     1441 agatccaggg cgacgcggtg cgcgtgagcg gcaagagcaa agatgagctg cagcgcctga
     1501 tcgcgcgact gcgccaggag tctgagggct atccggtgcc gctccagttc cagaactacc
     1561 gatgatcggc tccgatcgca ccgtccgggt ggtcatggcc atcgtggtcg tggtcgtcat
     1621 cctgggcctg atcctcagct ccatccgctt cgcgctctag gcggcgcatg gcccggcgct
     1681 cggggagaac agtcggccga ctcgtggccg acgccatcgc ggcggcaggg gtccgctggg
     1741 ccttcacggt gccgggcgag agcttcctcg ggctgctcga cgcgctgccc gcggccggca
     1801 ttcgcgtcgt ggccacgcga cacgagggcg gcgccgcgtt catggcggag gcggtgggcc
     1861 agctgaccgg ccagccggcc gcggtcctgg gcacgaggac ggtcggcgcg tccaacatgg
     1921 ccataggcat ccacaccgcg tacagcaact cgacgccgtt ggtcgctttg ctgggcgacg
     1981 tccagcgctc gcacaagggc cgcgaggcct tccaggagag cgatctcgtc gcgcgcttcg
     2041 gcgggctcgc caagtgggca gcgcagatcg t
//
LOCUS       DATBRX010000140         3832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_49002, whole genome shotgun sequence.
ACCESSION   DATBRX010000140 DATBRX010000000
VERSION     DATBRX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49002"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..677
                     /locus_tag="VIF08_05360"
     CDS             147..677
                     /locus_tag="VIF08_05360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEY8135451.1"
                     /translation="MEPLAVSVRPERPADRDAIRALHDLVFEEPDEGEIVDAIRGSAD
                     EIAGGSIVALDKSGAVIGHLLASRGFIVGSSGTQWPVWMLGPFAVRADLQGRGVGARL
                     MREQIDRARKRGVPLICLLGHASYYPRFGFLPARSMGIEPPQPWPDEHWQALKLPSYS
                     PDLHGVARYAPAFKVG"
     gene            complement(690..765)
                     /locus_tag="VIF08_05365"
     tRNA            complement(690..765)
                     /locus_tag="VIF08_05365"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:His,seq:gtg)
     gene            804..1172
                     /locus_tag="VIF08_05370"
     CDS             804..1172
                     /locus_tag="VIF08_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135452.1"
                     /translation="MNIPGFIARQFYVADSLRNTDSGWELKAQNPMGNGVLTGVGHLR
                     VDGREIPPESVTAQRSGDAEPIRAQDVTRQKPVSVFKGDHVTLHVSGAPLALGEHTLE
                     VELFELNLGRLSFSITDNVR"
     gene            1197..1886
                     /locus_tag="VIF08_05375"
     CDS             1197..1886
                     /locus_tag="VIF08_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEY8135453.1"
                     /translation="MAASTRWVRVPPVERGVTLGDFLVPIRLGERAASWQRHLLMIAV
                     GTLLIILGARISFYLPGNPLVPVTLQTFGVLFGGALLGFRRSLLSVGLYLLLGFVGLP
                     VFALDETTGLYRSGLSTVIGLESGQIVLGPSGGYLVGFLFAGGLVGRLAELGWDRKIG
                     GSIAAMLLGNLVVYLIGIPWLMAAAGLNLEDGLRYGLWDFVPGDLIKVAVAAALLPVG
                     WWLVKRRSSDL"
     gene            1930..2002
                     /locus_tag="VIF08_05380"
     tRNA            1930..2002
                     /locus_tag="VIF08_05380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1963..1965,aa:Arg,seq:tcg)
     gene            complement(2006..3115)
                     /locus_tag="VIF08_05385"
     CDS             complement(2006..3115)
                     /locus_tag="VIF08_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135454.1"
                     /translation="MRRPLSLLIAGAFSLTALSAAFLIDNSPASAAPIAGAPTTYCAD
                     PDTDPSIDTGAGTMITCDTTITNTITGIDPITGVASGSAVISVTECTGPASGRLDPSF
                     LTCTTDEQILVNLVTHIDQCNGVGYGGGNVLECNVDVINNFLGVTPEVISAATVNQCN
                     GSAPDTTGCNPFPAATTNATITQCNNSSYGGGQEDFNCDASGTTTASLSVTVDQCNDS
                     NYGGGSWLNCSASLTNNVVPLATSSPTLPSDTPTPTLPADTATPSLAPTPTLAPTPTS
                     AATPSLAPTPTLAATPSAAPTPTLAATPTLAAAPTLAATPAATPSALPDTAMARPTTP
                     PPTGGGLGLPLLMVALVSLAFPLWRIYSDRGAQVR"
     gene            3324..>3832
                     /locus_tag="VIF08_05390"
     CDS             3324..>3832
                     /locus_tag="VIF08_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135455.1"
                     /translation="MKDTALARPRSLRDIPSVTWSLLLVAVVLVAYELSQLSYSLIGA
                     LLTAAPVLFAAAVLYVRPADRRYTWAALALAVAPVVSLVWSALPELVFRYAPGDWRNA
                     AFFLQDMGPIVRDATSVITIAGLGLLGLALGGVRRPLSVAILGVSVVIAIVHAIAIAD
                     SYSAINYPPG"
BASE COUNT          586 a         1211 c         1341 g          694 t
ORIGIN      
        1 ttcgagctgg gccacgcggc aggcatgtcg ggcagcgcca gggagcagga cgagcgcgtc
       61 ctcgccgagg tctggcgcta gctccggcgg ctcgcgccac cagggcgggt ggcgtcagga
      121 caggctgagc ggctaacctg caagccatgg aaccgctggc cgtcagcgtg cgccccgagc
      181 gtcccgccga ccgggacgcg atccgcgcgc tccacgatct tgttttcgag gagcccgatg
      241 agggcgagat cgtcgacgcc atacgcggca gcgccgatga gatcgcgggc gggtccatcg
      301 tcgcgctcga caagagcggc gcggtcatcg gccatctgct ggcatcgcgc ggctttatcg
      361 tgggcagcag tgggacgcag tggccggtgt ggatgctcgg cccgttcgcg gtgcgcgccg
      421 acctccaggg ccgcggcgtg ggcgcgcggc tgatgcgcga gcagattgac cgcgcccgga
      481 aacggggcgt accgctcatc tgcctgctcg gccacgcttc gtactacccg cgtttcggct
      541 tcctgcccgc ccgatcgatg ggcatagagc cgccgcagcc ctggccggac gagcactggc
      601 aggccctcaa gctgccctca tacagccccg acctgcacgg cgtggcgcgc tacgcgcccg
      661 ccttcaaggt cggctaaccg cagggatgtt ggggtggcct atgggggtcg aacccataac
      721 cttcggatcc acaatccgat gctctgccgg ttgagctaag gccaccgcga gggggagtgc
      781 caagtatcgc ctaaccttgg caaatgaaca tcccgggctt catcgcgcgc cagttctacg
      841 tggccgattc gctgcgcaac accgactccg gttgggagct caaggcgcag aaccccatgg
      901 gcaatggcgt gctcacgggc gtcggccatc tgcgcgttga cgggcgcgag attccacccg
      961 aatcggtcac cgcgcagcgt tccggcgacg ccgagccgat ccgcgcccag gacgtgacgc
     1021 gccagaagcc agtgagcgtc ttcaagggcg accacgtcac gttgcacgtg agcggcgcac
     1081 cgcttgcact cggcgagcac acgctggaag tcgagctatt cgagctcaac ctgggccggc
     1141 tctcgttctc gatcaccgac aacgtgcgct agaccgtcac tggtagccta gggcgcatgg
     1201 ccgcctccac ccgctgggtg cgtgttccgc ccgtggagcg cggcgtaact ctgggcgact
     1261 tcctggtccc gatccgcctg ggcgagcgtg ccgcttcgtg gcagcgccac ctgctgatga
     1321 tcgcggtggg cacgctgctg atcatcctgg gcgcccgcat cagtttctat ctgccgggca
     1381 acccgctcgt gccggtcacc ctccagacgt tcggcgttct cttcggcggg gcgttgctgg
     1441 gcttccgacg ctcgctgctg tccgttgggc tgtacctgct gctgggcttc gtcggcctgc
     1501 ccgtattcgc gctcgatgag acgaccggcc tctaccggtc aggactcagc accgtgatcg
     1561 gcctcgaatc cgggcagatc gtgctcggtc cgagtggtgg gtacctggtc ggcttcctgt
     1621 tcgccggcgg gttggtcggc cgtctggctg aactcggctg ggaccgcaag atcggtggct
     1681 caatcgcagc gatgttgctg ggcaacctcg tggtctacct gatcggcatc ccctggctga
     1741 tggcagccgc gggcctcaat ctcgaggacg gtctgaggta cgggctgtgg gacttcgtgc
     1801 ccggcgatct gatcaaggtc gcagtcgcgg ccgccctatt gccggtgggc tggtggctgg
     1861 tcaaacgccg ctcgtccgat ctctagcaca gagccctagc ccgggcgcac gaccctacaa
     1921 ttcggacgtg cgcccgtagc tcagtggata gagcggatgg cttcggacca tcaggccgcg
     1981 ggttcgaatc ctgccgggcg cgcttctacc ggacctgcgc gccgcggtcg ctgtagatcc
     2041 tccacagcgg gaacgcgagc gataccaggg cgaccatcag aagcggcaat cccagccctc
     2101 cgcctgtcgg cggcggcgtc gtcggacgag ccattgccgt atccggaagc gccgacggag
     2161 ttgcggcagg cgtggccgcc agggtgggtg ctgctgccag tgttggcgtt gctgccaagg
     2221 tgggcgtcgg tgccgccgac ggagtagctg ccaaggtggg cgtcggtgcc aggctcggcg
     2281 tcgcagccga ggtgggcgtc ggtgccaacg taggcgttgg tgcgagcgag ggcgtcgccg
     2341 tgtctgcagg cagagtcggc gtcggggtat cgctcggaag cgtcggtgac gacgtcgcca
     2401 ggggcacgac gttgttggtc aggctggcgg agcagttgag ccacgagccg ccgccgtagt
     2461 tggagtcgtt gcactggtcg acggtcacgc tcaacgaagc agtggtcgta ccgctggcgt
     2521 cgcagttgaa gtcttcctgg ccgccgccgt agctggagtt gttgcactgc gtgatcgttg
     2581 cgtttgtggt ggcggcgggg aagggattgc agcctgtcgt gtccggcgcc gatccgttgc
     2641 actggttaac ggtggcggcg ctgatcactt cgggagtgac gccgaggaag ttgttgatca
     2701 cgtcgacgtt gcactcgagc acattgccgc cgccataacc gacaccgttg cactggtcaa
     2761 tgtgcgtgac gaggttgacg aggatctgct cgtccgtggt acaggtcaga aatgagggat
     2821 ctagacggcc gctcgcgggg ccggtgcact cggtgacgct gatgacggca ctcccgctgg
     2881 ctacaccggt gatcggatcg atgccagtga tggtgttcgt aatcgttgtg tcgcacgtga
     2941 tcatggtgcc cgcgccggta tcaatcgacg ggtcggtgtc tggatcagcg caataggtgg
     3001 tcggcgcgcc ggcgatcggt gctgcggacg cgggtgagtt atcgatcaga aaggccgcgc
     3061 tcagcgcggt aagggagaaa gcccctgcaa taagcaggga cagtggacga cgcacaatga
     3121 cacctggtga gggtcgagcg tcggagccaa attattcagc ggcggggccc gtgctcctca
     3181 ccgttggtgc gagactggga agagtcaacc cggaccatca gccggacagg ccgcgcacta
     3241 ccgcgcagcc cacaagaccg cacaggcgcg aacggatggg cggtgcccaa aagcaccggt
     3301 cgatcgaacg gaggggtgaa acgttgaagg acacagcatt ggcgcggccg aggagcctgc
     3361 gcgacattcc atcggtgacc tggtcactgc tcctggtcgc agttgtgctg gtcgcttacg
     3421 agctcagcca attgagttac tcgttgatcg gggcgctgct gacggccgcc cccgtcctgt
     3481 tcgccgccgc cgtcctctac gtcagacccg ctgaccggcg ctacacctgg gccgcgctcg
     3541 ccctggccgt agctcccgtc gtgagcctgg tgtggagcgc cctgccggag ttggtgttca
     3601 ggtacgcacc tggcgactgg cggaacgcgg cgttcttcct gcaggacatg ggccccatcg
     3661 tccgcgacgc gacgagcgtg atcacgatcg ccggccttgg cctgctcggt ttggcgttgg
     3721 gcggagtgcg caggcctttg tccgtggcaa tcttgggcgt gagcgtcgtc atcgcgatag
     3781 tgcacgcgat agccattgcc gactcctatt cggcaatcaa ctatccgccg gg
//
LOCUS       DATBRX010000141         7493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_719240, whole genome shotgun sequence.
ACCESSION   DATBRX010000141 DATBRX010000000
VERSION     DATBRX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719240"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VIF08_05395"
     CDS             <1..489
                     /locus_tag="VIF08_05395"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HEY8135456.1"
                     /translation="AEVILSNYDFGPRDTMMIFSASGLTAVPVEMARGARRRGLRVIA
                     VTSVAQSMSAEPDPGAGARLLDEADLVIDLCTPPADALSRVENLATPVGPGSTIAAVA
                     IVNSIKVRVAELLSERGQMPPVITRAAVVGQEQSSELFEEAYKEHARRLARVIARQGG
                     GG"
     gene            549..1685
                     /locus_tag="VIF08_05400"
     CDS             549..1685
                     /locus_tag="VIF08_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8135457.1"
                     /translation="MVKRTKLAVFGMLAGVLLIGVACAAPGASPTPAGSSVTLGYSNA
                     FGVGNGFREEQLCTAKAEALVKGVGVSTWTHAEDGTYPQLQQLTDLIAKSPDAIIFNP
                     NSPDGYQAVLDQAQAAGIKTIAIDAYVTDKETWNISNDQFNYGYVGANWLFKKLGGQG
                     NVYYMRGIVGHPADLDRHDGVMKALSENPGIHLLPSNDGVGTDWNPDKGTQLMQGIIN
                     DGTYDTIDGIWTSGIDQQVVDAIQASGKPFVPVVGADLKGFVEQLLHKDPKYNGLEGI
                     AVYNPASIGGAGVNLALKVLNGESPATTTIKRTTADGVEHDIKVVLLPVPEAYANDTA
                     DGMAKLEQINVAGLNVLWPVSWSITGWTDYTLDQMLACKGPGEP"
     gene            1768..3327
                     /locus_tag="VIF08_05405"
     CDS             1768..3327
                     /locus_tag="VIF08_05405"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY8135458.1"
                     /translation="MTATAIPAALLLEATDVSKAYGVVVALKSASLAVKPGEVHALMG
                     ANGAGKSTLVKILSGAVRPDTGRILVRGREAAARSPGEARRNGIVSVYQEPALIPDLD
                     LRSNLRLTGTPVGAFLHWANELGLSNVDLSDVALTLPLATQRVIDLARALAIEPDVLM
                     LDEMTAALPANLTERVLEVVARLRGGERSVIFISHRMIEIAAVCDRATVLREGETVGV
                     VDVTPGSEERIVQLMLGEIAGQAPATTSADVYAATKRQRDLVPRLQVKGLAAGTHLND
                     VDFELYPGEVLGVVALEGQGQDELFDVLCGATRASAGEVIVEGSPVNFRHPADAIRAG
                     VVYVAADRAEALLMQRNVRENITLPSSALPRHWGPIDLGKERRRVNAAVETLQIDARA
                     GAEVRRLSGGNQQKVTIARWIAGGVKTMLCFDPTRGIDIRTKNQIYNLLRDLVAAGAA
                     VLLYTSELKEIQLACDRVIVIFGGRVVAELPAADADEAALLRAAYNLKQEETLPEDVA
                     AEVVAAEERAE"
     gene            3324..4325
                     /locus_tag="VIF08_05410"
     CDS             3324..4325
                     /locus_tag="VIF08_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8135459.1"
                     /translation="MTTISPPTEVRPTFAAVARASFGRDAWVLGLAGLVILLLFFTRI
                     INPNYGAGGLQSLSVNVLPLAFAAIAQTIVVISGGIDLSIASMMALTNMVAATLMLGR
                     SDEIGIGVFVGVLVLGVLLGAVNGMLIVWTRVADIIVTLAMSFVWAGFALWIAQAPVR
                     SPHDWLSGLVNGSVLVDFLPKAFIVLLVSVSVVWIPLRRSRHGLALYAVGSNALAAFR
                     SGVSVGRTKVLAYALAGLFAGMGGLALSAITGQGNPLPGGFTLASVAAVVLGGVSLAG
                     GRGGVVGPILAVAVLSLIQTDMTFLGLNSNLATVTQGVILIAVVVLGSLTLRRRARA"
     gene            4322..5311
                     /locus_tag="VIF08_05415"
     CDS             4322..5311
                     /locus_tag="VIF08_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8135460.1"
                     /translation="MTGVQVERRQPAIASIARMFRDQPLIPLLMLLLALVAAFAIVRP
                     GADFPGFIAAQIRTAIPLAILAGCQTLVMLTGGIDLSVAATGSMAGFVAASLYTGPDS
                     LFVAIGVAFLFAALIGAINGLGVGVFRVHPLIMTLGMGLVILGAANAYQLQVVRTRSG
                     VPPELRWLGSATEGTFFPDSLLVFVPLTIVIIFMLRRTGFGRLLYALGDNPIAARLSG
                     VKSWQVLLALYVISALLAAVAGFLVSGLTNVASVTLVNPRLLPSVAAAVIGGTSIFGG
                     RGGFAGTIVGALILTVADSLLSTLGFPEAARQVLFGSIIVVVAAFYSRLTTES"
     gene            5315..6244
                     /locus_tag="VIF08_05420"
     CDS             5315..6244
                     /locus_tag="VIF08_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8135461.1"
                     /translation="MAPAPATRHLGLDLGATNIKTAVVERDGAAWRTLDHGQVPTHDD
                     AGPEAVLEQLIEAGRKAMAEHPDIASVGIGVPGLYYAERGATKFLVNMLGEWDGVPVG
                     PRVGAALGLPTHLVNDARAFGLAELRLGAGRGARSMVGLTLGTGIGGVIAIDGHIVQG
                     HDGTAGELGHHTIDPDGPPCNCGNNGCLESFARADRLAEACGASNAREAINRARAGDQ
                     AALAGMASVGRYLGIGIANMITVITPDRVVIGGGIAAAGNLLLDPIRAEVARRVHVTS
                     LDKVTIALAELGTWAGAIGAAIHGAETAETMAS"
     gene            6241..7356
                     /gene="nagA"
                     /locus_tag="VIF08_05425"
     CDS             6241..7356
                     /gene="nagA"
                     /locus_tag="VIF08_05425"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY8135462.1"
                     /translation="MSERITGRLILDDRVAVGSLTVEDGLITDVQAGPDSNDGGPYIS
                     PGFVDVHVHGWGGHSAMGDRAALDGMARSLLRRGVTSFLPTAVSAPLDELAAFASRVR
                     DWMPAAPGDGAEPLGFNLEGPFLAQARRGAHDPSFLMSPADVASEQLTPLLDSLRVIT
                     IAPELLGALPLIASLAGRGVGVSLGHSGANLVEARDGYAAGARGTTHLFNAMTGVDHR
                     APGLAVAALLDDRVYTELIADGIHVDRELWPLITRLKPADRLLLVSDAIPFAGTGDGR
                     GRLGSLDIEIAGERVTLAGTQTLAGSVLALDKAVRNMFAAGIALPAAVAAATRNPLAM
                     LGISDRGRLAPGQRADLIELDAELNVKRVMRAGEWVS"
BASE COUNT         1152 a         2516 c         2528 g         1297 t
ORIGIN      
        1 gccgaggtga tcctgtcgaa ctacgacttt gggccgcgcg acacgatgat gatcttctca
       61 gccagcggcc tgaccgcggt gcccgtcgag atggcgcgcg gcgcccgccg gcgcgggcta
      121 cgcgtcatcg cggttacaag cgtggcgcag tcgatgtcgg ccgagccgga tcccggcgcc
      181 ggcgcgcgcc tcctcgacga ggcggacctc gtgatcgacc tgtgcacccc gcctgccgac
      241 gccctgtcgc gagtcgaaaa cctggctacg ccggttggcc cggggtctac catcgctgcg
      301 gtcgcaatcg tcaactccat caaggttcgc gtggcagagc tcctctcgga gaggggacag
      361 atgccgcccg ttatcactcg ggcggcggta gtcggccagg agcagtcaag cgaactcttc
      421 gaagaggcat acaaagagca cgcacggcga ctcgcccgcg tgatcgccag acaaggagga
      481 ggtggctgac gggagaggcc ttgcacagtc cgcgattgat tgggacacac ggaggaggaa
      541 ttcacgtaat ggtcaaaagg accaagctag cggtcttcgg catgctggcc ggcgtactgc
      601 tgatcggcgt cgcgtgtgcg gcccccgggg cctcgcctac gcctgccggc tcgagcgtca
      661 cgctcggcta ctcgaacgcg ttcggcgtcg gcaacggctt ccgcgaggag cagctgtgca
      721 cggccaaggc agaagcgctc gtcaagggcg ttggcgtatc cacgtggaca catgccgagg
      781 acgggaccta tccgcagctc cagcagctga cggacctgat cgccaagtcg cccgacgcga
      841 tcatcttcaa cccgaatagc cccgacggct atcaggccgt gctcgaccag gcgcaggctg
      901 ccggcatcaa gacgattgcc atcgacgcct acgtcaccga taaggagacc tggaatatct
      961 ccaacgacca gttcaactac ggctacgtag gtgcgaactg gctgttcaag aagctcggcg
     1021 gccagggcaa cgtctactac atgcgcggca tcgttggcca cccagccgac cttgaccggc
     1081 acgacggcgt catgaaggcg ctctcggaga acccgggcat ccacctgctg ccgagcaacg
     1141 acggtgtggg caccgactgg aatcccgaca agggcaccca gctgatgcag ggcatcatca
     1201 acgacggcac gtacgacacc atcgacggca tctggacatc gggcatcgac cagcaggtcg
     1261 ttgacgcgat ccaggcctcc ggcaagccgt tcgtccccgt cgtcggagcg gacctgaagg
     1321 gctttgtcga gcagctgctc cacaaggatc ccaagtacaa cggcctcgag ggcatcgccg
     1381 tctacaaccc ggcatccatc ggcggcgccg gcgtcaatct ggcgctcaag gtcctcaatg
     1441 gcgagtcccc ggcaacgaca acgatcaagc gcacgactgc cgacggtgtc gagcacgaca
     1501 tcaaggtcgt cctgctccca gtgcctgagg cgtacgccaa cgacactgcg gacggcatgg
     1561 cgaagctcga gcagatcaat gtcgctggtc tgaacgtgct atggccggtc agctggtcaa
     1621 tcaccggctg gacggactac acgctcgatc agatgctcgc ctgcaagggt ccgggcgaac
     1681 cataacccgg aaggttcttt cctggacaga tggggatgcc gagcaatcgg catcccctct
     1741 ctgttcttgt accttgcgga cgtaccgatg acggcaactg ccatcccagc cgcactgctg
     1801 ctcgaggcta ccgatgtctc gaaggcatac ggcgtcgtcg ttgccctgaa gagcgcgtca
     1861 ttggctgtga agccaggcga ggtgcacgcc ctgatgggcg cgaacggcgc cggcaagagc
     1921 actcttgtga agatcctgtc tggtgccgtg cgccccgaca cggggcgcat cctcgttcgt
     1981 gggcgagagg ctgctgcacg atcaccgggc gaggcgcggc gcaacggcat cgtgtccgtc
     2041 tatcaagagc cagcgctcat tccagatctc gacctgcgct ccaatctgcg cctgaccggc
     2101 actccagtcg gcgcctttct gcactgggcc aacgagctcg ggctgagcaa cgtcgacctc
     2161 tccgacgtgg ccctgaccct tcccctggcc acccagcgcg tgatcgatct cgcccgcgcg
     2221 ctggctatcg agccggacgt tctcatgctc gacgagatga ctgcggcgtt gccggccaac
     2281 ctgaccgagc gcgtactcga ggtggtggcc cggctgcggg gcggcgagag atcggtgatc
     2341 ttcatttcgc accgaatgat cgagatcgcc gcggtctgtg accgagcgac ggtcctgcgt
     2401 gaaggcgaga cggtcggggt ggtcgacgtg acgccgggat ccgaggagcg catagtccag
     2461 ctgatgctcg gcgagatcgc cggccaggca ccggcaacca cgtccgccga cgtgtacgcc
     2521 gcgaccaagc gccagcggga cctggtgccg cgtctgcagg tgaaggggct ggcggcgggc
     2581 acgcacctca atgacgtcga tttcgagttg tacccgggcg aggtgctcgg cgtcgtcgcc
     2641 ctcgagggcc agggccagga tgagctgttt gacgtcctgt gtggcgccac tcgagcaagc
     2701 gcgggcgagg tcatcgtcga gggctcaccg gtaaatttcc ggcacccggc ggacgccatc
     2761 cgagcgggcg tcgtctatgt cgccgcggac cgtgccgaag cgcttctcat gcagcgcaat
     2821 gtgcgtgaga acatcactct gccgtcgagc gcactgcccc ggcactgggg gcccatcgac
     2881 ctgggcaagg agcgcaggcg cgtcaacgcc gcggtcgaga cgctgcagat cgatgcgcgg
     2941 gccggggccg aggtgcgccg cctgtcaggt ggcaaccaac agaaagtgac gatcgcccgc
     3001 tggatcgcgg gcggggtgaa gacgatgctc tgcttcgacc cgacgcgcgg catcgacatc
     3061 cggaccaaga atcagatcta caacctgctg cgcgacctgg tggcggctgg tgccgcggtc
     3121 ctgctgtaca catccgagct caaggagatc cagctcgcgt gcgaccgcgt gatcgtgata
     3181 ttcggtgggc gtgtggtcgc cgagctccct gccgccgatg ccgacgaagc ggcgctcctg
     3241 cgcgcggcgt acaacttgaa gcaggaggaa accctgcctg aggacgtggc cgccgaggta
     3301 gtcgccgccg aggagcgggc cgaatgacca caatcagccc gcctaccgag gtgcgaccga
     3361 cgtttgcggc ggtcgcccgc gcgtcgttcg gccgcgacgc ctgggttctt ggcctcgccg
     3421 gcctggtcat cctgctgctc ttcttcacgc ggatcatcaa tcccaactac ggcgccggcg
     3481 gtctgcagag cctgtcggtc aacgtgctgc ccctcgcgtt cgcggccata gcgcagacga
     3541 tcgtcgtgat ctcgggcggc attgacctgt cgatcgcctc gatgatggcc ctgaccaaca
     3601 tggtcgccgc gaccttgatg ctgggccgca gtgacgagat cggtatcggc gtgttcgtcg
     3661 gggtcctcgt gctgggggtc ctcctcggag ccgtgaacgg catgctgatc gtctggacgc
     3721 gcgtggccga catcatcgtc acgcttgcca tgtccttcgt ctgggcaggc ttcgccctct
     3781 ggattgcgca ggcccccgtt agatcgccgc acgattggct gtcgggcctc gtaaacggct
     3841 cggtccttgt cgatttcctg cccaaggcct tcatcgtcct gctggtctcg gtcagcgtgg
     3901 tctggatacc cctgcgtcga tcgcgccacg gcctcgcgct ctatgccgtt ggcagcaacg
     3961 cgcttgccgc attccgcagc ggcgtctccg tgggtcggac caaggttctc gcctatgcgc
     4021 tggccgggct gtttgccggg atggggggtc tggcgctgag cgctatcaca ggccagggca
     4081 atccgttgcc cggcggcttc acgctggcca gcgtggccgc agtcgtcttg ggtggagtga
     4141 gcctcgcggg cggacgtggt ggcgtggtcg gcccgatcct ggcggtcgcc gtcctgtcgc
     4201 tcatccagac ggacatgacg ttcctgggcc tgaactcgaa cctcgcgacg gtgacgcagg
     4261 gcgtgatcct gatcgcggtc gtggtcctgg gaagcctcac gttgcggcgg cgggctagag
     4321 catgaccggc gtccaggtgg agcgacgtca acccgccatc gcctcgatcg cgcgcatgtt
     4381 ccgcgaccag ccgctgatac cgctgctcat gctgctgctg gcgcttgtcg ccgcctttgc
     4441 aatcgtcagg cccggcgcgg acttcccggg gttcatagcg gctcagatcc gcacggccat
     4501 tccactggcg atcctcgccg ggtgccagac cctggtcatg ctcactggcg gcatcgatct
     4561 gtcggtggcg gcgacggggt ccatggctgg cttcgtcgca gcgtcgctct acaccggacc
     4621 cgacagcctg ttcgtcgcga tcggggtggc gttcctattc gcggctctga tcggggcgat
     4681 caatggtctt ggcgtaggtg tattccgggt ccacccgttg atcatgaccc tcgggatggg
     4741 cctggtcatc ctcggcgcgg ccaatgcgta ccaattgcag gtcgtgcgca cccgtagcgg
     4801 cgtgccgccc gagctgcgct ggctcggctc ggcgacggag gggaccttct tccccgacag
     4861 cctgctcgtg ttcgtgccgc tcaccatcgt catcatcttc atgctgcgtc gcaccggttt
     4921 tggcaggctc ctctacgcgc tcggggacaa ccccatcgcg gcgcgcctgt cgggcgtcaa
     4981 gagctggcag gttctcttgg cgctgtacgt gatttcggcc ctgcttgcgg ccgtcgcagg
     5041 attccttgtc tcgggcctga ccaacgtggc cagcgtcacc ctggtcaacc cgcgacttct
     5101 cccgtcagtt gccgccgcgg tcatcggtgg gacctcgatt ttcggaggcc gaggtggttt
     5161 cgccggcacc atcgtcggcg cgttgatcct gaccgtcgcc gattcgctgc tctcaacgct
     5221 cggctttccc gaagccgctc ggcaggtatt gttcgggtcc atcatcgtcg tcgtcgcggc
     5281 cttctactcc cgcctgacga ccgagtctta ggcagtggcg ccggcgccgg ccacgcgcca
     5341 tctgggcctt gatctgggcg ccaccaacat caagaccgcg gtcgtggaac gcgacggtgc
     5401 tgcgtggcgc acgcttgacc acggccaggt tccgactcac gacgacgccg gccccgaggc
     5461 agtcctggag cagctcatcg aggccggccg gaaggcgatg gccgagcatc ccgacatcgc
     5521 ttcggtaggc ataggcgtgc cgggtctcta ttacgcggaa cgcggcgcga ccaaattcct
     5581 cgtcaacatg ttgggcgagt gggatggcgt gccggtcggt ccaagggtcg gcgccgcact
     5641 cggattgccg actcatctcg tcaacgacgc gcgcgcattt gggctcgcgg agctgcgcct
     5701 gggcgctggg cgcggcgcgc gctcgatggt cggcctgacg ctgggcacgg gcatcggcgg
     5761 cgtcatcgcc atcgacggcc acatcgtcca gggtcatgac gggaccgcgg gcgagctggg
     5821 ccaccacacg atcgatcccg acgggccacc gtgcaactgc ggtaacaacg gctgcctcga
     5881 gtcgttcgcc cgggcagacc ggctggcgga ggcgtgcggt gcctctaacg cgcgagaggc
     5941 gatcaaccgt gcgcgtgccg gcgaccaggc ggcgctggcg ggcatggcgt cggtcggccg
     6001 atatctgggc atcggcatcg ccaacatgat caccgtgatc acgcccgacc gggtcgtaat
     6061 cgggggcggc atcgccgcgg ccgggaacct gctgctcgat cccatccggg cggaggtcgc
     6121 gcgccgcgtc catgtcacgt cgctcgacaa ggtgaccatc gcgctcgccg agctgggcac
     6181 gtgggccggg gcgatcggcg cggcgatcca cggcgcggag accgcggaga ccatggcctc
     6241 atgagtgagc gcatcaccgg ccgattgatc ctggacgacc gagtcgccgt tggttcgctg
     6301 acggtcgagg acgggctgat caccgacgtc caggccggtc ccgattcgaa cgacggtggc
     6361 ccgtacatca gccccggctt cgtcgacgtt cacgttcacg gttggggcgg ccactcggcg
     6421 atgggcgacc gcgcggcgct cgacgggatg gcgcggtcgt tgctgcggcg cggcgtcaca
     6481 tcgttcctgc cgacggccgt gtccgcgccg ctcgacgagc tggccgcatt cgcgtcgcgt
     6541 gttcgtgact ggatgccggc ggctccggga gacggcgcag agccgcttgg cttcaacctg
     6601 gaggggccgt tcctcgcgca ggcgcggcgc ggcgcccacg acccatcttt cctgatgtcg
     6661 ccggcggatg tggcgagcga gcagctcacg ccactgctgg actcgctccg cgtcatcacc
     6721 atcgcgcccg agctgctggg cgcgctgccc ttgatcgcgt cgctggccgg acgcggtgtc
     6781 ggggtgtcgc tgggccactc gggcgcgaac ctggtcgagg cgcgcgacgg ttacgccgca
     6841 ggggcgcggg gcacgacgca tctgttcaac gccatgaccg gtgtcgatca ccgcgccccc
     6901 ggcctggccg tcgcggcctt gctggatgac cgcgtctaca ccgagctcat cgccgacggc
     6961 atccacgtcg accgcgagct ctggccgctc atcacgcggc tcaagcccgc tgatcggctg
     7021 ctgctggtca gcgacgccat cccgttcgcc ggcaccggcg acggccgcgg ccgcctgggc
     7081 tcgctggaca tcgagatcgc cggggagcgc gtcacgctcg ccggcacgca gacgttggcc
     7141 gggtcggtgc tcgccctgga caaggcggtg cgcaacatgt tcgccgccgg tattgcgcta
     7201 ccagccgcgg tcgccgcggc gacgcgcaac cccctggcga tgctcggcat aagcgatcgc
     7261 ggccgcctgg cgcccgggca gcgcgccgat ctcatcgagc tcgacgccga actcaacgtc
     7321 aagcgcgtca tgcgcgctgg ggagtgggtc agctagggcc ctcggccgtc gcggaactgg
     7381 ccagatgatc gatccgcggg cgctattcgg acacttggtc atccttcatg gttcgccaat
     7441 gcccagtggg ccccgccgat gccgtctgag agggcgctat ctggtcaaac gct
//
LOCUS       DATBRX010000142         5230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2282747, whole genome shotgun sequence.
ACCESSION   DATBRX010000142 DATBRX010000000
VERSION     DATBRX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282747"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..516)
                     /locus_tag="VIF08_05430"
     CDS             complement(115..516)
                     /locus_tag="VIF08_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135463.1"
                     /translation="MIGNIRRHAIRWFAVALIGAGLVSFPAGVAAKDTDVIRTGSCSG
                     ASDWKLKAKPDNGRLEVEFEVDQNVNNRTWSVKLKKNGNVFWQGQRTTHAPSGSFSVT
                     KFTGNPAGTDTIVGRAVNPATGEVCQGSLTI"
     gene            713..2110
                     /locus_tag="VIF08_05435"
     CDS             713..2110
                     /locus_tag="VIF08_05435"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8135464.1"
                     /translation="MSDSPVKRLSGLLGLNQSSSLARRFLLASLAVLMVGGLTVGWWI
                     GGQLERGIVDRTASITGLYVQSFIEPHLESLASDSWLSDDDIADLDDLLGDTAFGQRV
                     VALKVWRPDGAIVYSPDRSLIGQSYPVEDGLRDAVDGNVVAEMSALEADENVSEQARG
                     FSRLLEMYLPVRERGGGRIIAVAEFYVLPTEIDQEVGGAQLRSWLAVGLAMALVYLLL
                     FGIVRQGSQTIDRQRLALEAQVAELSTLLEQNEQLRGRVRVAAERTTTLGERNLRRIS
                     SDLHDGPGQMLALAMLRLDQLRAGDGKVDERELAELQSALNDALRDMRAIAAGLRLPE
                     LSSLSTADTLRRAVDDHVRRTGVPVALTLSEPPREASLPTKIALFRAAQELLSNATRH
                     GGGHDVALNLEAADGRLVLTVADGGPGFGDTRVGEGGHLGLAGIREQAELLGGSFEIG
                     ARPDAGAQVTVRWPL"
     gene            2098..2787
                     /locus_tag="VIF08_05440"
     CDS             2098..2787
                     /locus_tag="VIF08_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8135465.1"
                     /translation="MAAVNAAATDRIRIVVADDHPMFRAGVVASLAAQPDIEVVGEGA
                     SAAEALNLVAQHSPDVALLDIAMPGGGLSAARDISAASPATRVVMLTVSEDEDDLLAA
                     MKAGASGYVLKGAAASELVNVLRTVNAGEVYVAPGLAWGLLREMSRPRSAPLDELSAR
                     EHEVLELVAAGLSNQEIGDRLSLAEKTIKHYMTSILSKLRVRSRVEAALLAYREGMAP
                     PVSEPRDEGRE"
     gene            complement(2818..3291)
                     /locus_tag="VIF08_05445"
     CDS             complement(2818..3291)
                     /locus_tag="VIF08_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135466.1"
                     /translation="MTLGGLFPFVLFAHIVLAISLFMPTILLPFALRLKGGKFEQGPG
                     RFSRALFWLQRNGSWLIGAGLAGTGIALVLTLGVQLLAQPWLVLALTLYAANLLIAFV
                     IQRPGLARLLRLQPGDSEAARRRWRDRARRQRYFSYVMAGLIGVIAFLMSTKPEL"
     gene            complement(3288..4265)
                     /gene="prmA"
                     /locus_tag="VIF08_05450"
     CDS             complement(3288..4265)
                     /gene="prmA"
                     /locus_tag="VIF08_05450"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY8135467.1"
                     /translation="MAGCDQLIDNEGVSAAGSWYELSVEADQEAVEQVSEILSRACPG
                     GVSVEAPFVPVQEGLAARIDATRPAIVRGYISAIDASAVALAVHRVREALGHLQAFEL
                     RPIGDLQTRVVHEEDWAEAWKEHFPVMRIGKRLVIRPTWREHEPEPSDVVLGLDPGMA
                     FGTGLHPTTRLCLAGIEHWADDALLSDARVLDVGTGSGILAIAAALMGASYVLGIDTD
                     PLAVETTKANAATNGMADFIEAHTGSLPLPEPAQFHLVVANLISGLLIDLAAELAATL
                     WPGGRLLAGGIYRDREPEVRAAFESAGLRVVGRMAEEDWVALELEPAEG"
     gene            complement(4244..>5230)
                     /locus_tag="VIF08_05455"
     CDS             complement(4244..>5230)
                     /locus_tag="VIF08_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY8135468.1"
                     /translation="VGGAGFDPFGGAGGQGFSGFGDIFDAFFGGAAAGARRPRVPTGA
                     DLRYDLELTFAESISGTEKEIEFTSLARCETCSGSGAEPGTQPTNCPKCGGTGELRQV
                     RSTMLGQMINVSPCDRCRGTGKIVESPCHTCRGDGRVERKRNLRVTIPAGIAEGHQVR
                     LSGEGEGAPRGGVPGNLYVVVHVAPHPQLRRQETELFYELPLSITQAALGARVRVPTA
                     TGSEEIEIKAGTQPGAEIRLRGRGVPHLRRPDSRGDLHVLVDVKVPQRLSARQRELLE
                     QLSAELGEVEEATDSDAGAGTDSTAGAAKPRKSGRRRGRQPGLRDRLRDAIS"
BASE COUNT          778 a         1810 c         1835 g          807 t
ORIGIN      
        1 ggtcttgtcc atcaggccga tgttagacag ccgggctggg gatcggtact cacatacgaa
       61 tgacgcggcc ccgctttcgc ggggccgcgc ccggactacc tggtggcgac gggtctagat
      121 ggtgagcgag ccctggcaga cctcgcccgt ggccgggttc acggcgcgac cgacgatggt
      181 gtccgtgccg gccgggttgc cggtgaactt cgtgacgctg aaagagccac tcggcgcgtg
      241 ggtcgtccgc tggccctgcc agaagacgtt gccgttcttc ttgagcttga cgctccacgt
      301 cctgttgttg acgttctggt ccacttcgaa ctcgacctcg agcctgccgt tgtccggctt
      361 ggccttgagc ttccagtcgc tcgcgccgct gcacgaaccg gtccgaatca cgtcagtgtc
      421 cttggcggcg acgccggccg gaaagctgac caggcccgcg ccgatgagcg cgacggcgaa
      481 ccagcggatg gcgtggcggc gaatgtttcc gatcatggcc cttgctcctc tttgcgctgt
      541 ctctgtgttg cgggtggtgt gtttcgcccc ccgggttgtg actcgatagt cggccgggcg
      601 gccgttgcgc cgccacgggc gcgggtcggg aaattggacg gaccttggtc tgatgccctt
      661 cgcgcccttg gtccataggt ccgcgcgcga gccgggacta cgatcagccg cgatgtccga
      721 ttcacccgtc aagcggctgt ccggccttct cggcctgaac cagtcgagct ccctcgcgcg
      781 ccgcttcctg ctggccagcc tggccgtgct gatggtcggt ggcctgaccg tgggctggtg
      841 gatcggtggc cagctcgagc gcggcatagt cgatcgcacc gcgtcaatca ccgggctcta
      901 tgtgcagagc ttcatcgagc cgcacctcga atcgctcgcg agcgacagct ggctgagcga
      961 cgacgacatc gccgatctcg acgatctgct gggcgacacc gcgttcgggc aacgtgtcgt
     1021 ggccctcaag gtctggcggc ctgacggggc gatcgtgtac agccccgacc gctcgctgat
     1081 cggccaaagt tacccggttg aagatggcct gcgcgacgcc gtcgacggca atgtcgtggc
     1141 cgagatgagc gcgctcgagg ccgatgagaa cgtgagcgaa caggcacgcg gcttcagccg
     1201 gctgttggag atgtacctgc cggtgcgcga gcgcggcggc ggccggatca tcgccgtcgc
     1261 cgagttctat gtcctgccaa ccgagatcga ccaggaagtc ggcggggcgc agctgcgctc
     1321 gtggctggcg gtaggcctcg cgatggcgct ggtctacctg ctgctattcg gcatcgtccg
     1381 ccagggcagc cagaccatcg atcgccagcg gctcgccctc gaagcgcagg tggccgagct
     1441 gtcgacgctg ctcgagcaga acgaacagct gcgcggccgg gtccgagtcg ccgcggaacg
     1501 cacgacgacg ctgggcgagc gcaacctgcg ccgcatctcg agcgacctgc atgacggtcc
     1561 gggccagatg ctggcactgg ccatgctccg cctggaccag ctgcgtgccg gcgacggcaa
     1621 ggtggacgag cgggagctgg ccgagctgca gtcggcgctg aacgacgcgt tgcgcgacat
     1681 gcgcgccatt gccgcgggcc tgcgcctgcc cgagctgtcg tcgctctcga ctgccgacac
     1741 actccggcga gcggtcgatg atcacgtgcg ccggaccggt gtccccgtgg cgctcacgtt
     1801 gagcgagccg ccgcgcgagg cgagcctgcc gaccaagatc gcgctcttcc gcgcggccca
     1861 ggagctgctc tccaacgcga cgcgccacgg cggcggccac gatgtggcgc tcaacctgga
     1921 agccgcggat gggcggctcg tcctgaccgt cgcggatggc gggccgggct ttggtgatac
     1981 acgcgtcggt gagggcggcc accttggcct ggccggcatt cgcgagcagg cagagctgct
     2041 cggcggcagc ttcgagattg gtgcgcggcc cgacgccgga gcacaggtga cggtacggtg
     2101 gccgctgtga acgccgcggc cacagaccgg atccgcatcg tggttgccga cgatcacccc
     2161 atgttccgag cgggcgtggt cgcttcgctc gccgcgcaac cggacatcga ggtcgtgggt
     2221 gagggcgcct ccgcggcaga ggcactcaac ctggtcgcgc agcattcacc ggatgtagcg
     2281 ctgcttgaca tcgcgatgcc cggtggcggc ctgagcgccg cgcgcgacat cagcgccgcc
     2341 tcgcccgcga cgcgggtcgt gatgctgacc gtgtccgaag acgaggatga cctgctcgca
     2401 gcgatgaaag ccggcgcgtc ggggtacgtc ctcaaaggtg ccgccgcgtc cgagctggtc
     2461 aacgtcctgc gcaccgtcaa tgcgggcgag gtgtacgtcg cgcccggcct cgcgtggggc
     2521 ctgctgcgcg agatgagccg gcccaggtcg gcgccgctcg acgagctgtc ggcgcgcgag
     2581 catgaggtgc tcgagctggt ggccgcgggg ttgagcaacc aggagatcgg ggatcgcctg
     2641 tcgctcgccg agaagacgat caagcactac atgacgagca tcctgtccaa gctgcgcgtg
     2701 cgcagccgcg tcgaagccgc tctgctcgcc tatcgcgagg gaatggcgcc gccggtcagt
     2761 gagccgcgcg acgagggccg cgagtagcgc gacagttgtg agccgggccc ggcgcgccta
     2821 gagctcgggc ttggtgctca tcaggaaggc gatgaccccg atcagcccgg ccatcacgta
     2881 gctgaagtag cgctgccgcc gcgcccggtc gcgccaccgc cggcgcgccg cctcgctgtc
     2941 cccgggttgc agccgcagga ggcgcgccag tccgggtcgc tggatgacga atgcgatcag
     3001 caggttggcg gcatagaggg tcagcgcgag caccagccac ggttgggcga gcaactgcac
     3061 acccagggtc aggacgaggg cgatgcccgt acccgcgaga cccgcgccga tcagccacga
     3121 gccattgcgc tgcagccaga acagggcgcg cgagaagcgg cccggaccct gctcgaactt
     3181 gccgcccttc agacgcaggg cgaacgggag caggatcgtc ggcatgaaga ggctgatcgc
     3241 cagcacgatg tgggcgaaaa ggacgaaggg aaacagcccg cccagggtca tccctcagcg
     3301 ggctccagct cgagcgcaac ccaatcctct tccgccatcc gtccgacgac ccgcaggccg
     3361 gccgattcga aggctgcccg gacctctggc tcacgatccc tgtagatgcc acccgcgagc
     3421 aaccgaccac ccggccagag ggttgccgcc agctccgctg ccaggtcaat gagcaggccg
     3481 gagatgaggt tggcgacgac gaggtggaac tgcgccggct cgggcagcgg caggctgccg
     3541 gtgtgcgcct cgatgaagtc ggccatgccg ttcgtcgcgg cgttggcctt cgtcgtttcg
     3601 acggccagcg ggtcggtgtc gatgcccagc acgtaggacg cgcccatcag cgccgcggca
     3661 atggcgagga tgccggagcc cgtgccaaca tcgagcacgc gcgcgtcgga cagcagcgcg
     3721 tcatccgccc agtgttcgat gcccgccagg cacagccggg tcgtcgggtg aagaccggtg
     3781 ccgaaggcca tgcccggatc gaggcccaag accacgtccg agggctcggg ttcgtgctcg
     3841 cgccacgtgg gcctgatgac caggcgcttg ccgatgcgca ttaccgggaa gtgctccttc
     3901 cacgcctcgg cccagtcctc ctcgtggacg acgcgcgtct gcaggtcgcc gatcggccgg
     3961 agctcgaacg cttgaaggtg acccagcgcc tcgcgcacgc ggtggacggc caacgcaacc
     4021 gcggaggcgt ctatcgccga gatgtagccg cgcacgatgg ccggccgcgt cgcatcgatg
     4081 cgcgcggcga ggccctcctg gaccggcacg aacggtgcct cgacgctcac gccgcccggg
     4141 caggcgcgtg acaggatctc gctcacctgc tcgactgcct cctgatcggc ctcgacagac
     4201 agctcgtacc agctgcccgc ggcagaaact ccctcgttat cgatcagctg atcgcatccc
     4261 gcaatcggtc gcgcagcccg ggctggcgcc cgcggcgtcg gccggacttc cgtggcttgg
     4321 cagcacccgc ggtcgaatcg gtgcccgctc ccgcgtcgga gtcggtggcc tcttcgacct
     4381 cgcccagctc ggccgacagc tgctcgagca gctcgcgctg cctggcgctc aggcgctgcg
     4441 gcaccttgac gtcgaccagc acgtgcaggt cgccgcgcga atccggccgg cgcagatggg
     4501 gcacgccccg gccgcgcaac cggatctctg cgccgggctg agtgcccgcc ttgatctcga
     4561 tctcttcgct gccggtggcc gtcggcaccc gtactcgcgc gcccagcgcg gcctgggtga
     4621 tcgaaagcgg cagctcgtag aagagctccg tctcctggcg gcgcagctgc gggtgggggg
     4681 cgacgtgaac gacgacgtac aagttgcccg gcacccctcc ccggggcgca ccctcgccct
     4741 cgcccgagag gcgcacctgg tggccctcgg cgatgcccgc cgggatagtc acgcgcaggt
     4801 tgcgcttgcg ctcgacgcga ccgtcgcccc ggcacgtgtg acacggcgat tcgacgatct
     4861 tgcccgtgcc ccggcagcgg tcgcatgggc tgacgttgat catctggccg agcatcgtcg
     4921 agcgcacctg gcgcagctcg cccgtgccgc cgcacttggg gcagttggtg ggttgcgtgc
     4981 ctggctcggc gccggagccg ctgcacgtct cgcagcgcgc cagcgaggtg aactcgatct
     5041 ccttttccgt gccgctgatc gactcggcga aggtcagctc gaggtcgtag cgcaggtctg
     5101 cgccggtcgg cacacggggc cgccgcgcgc ctgccgcggc accaccgaag aacgcgtcga
     5161 agatgtcgcc gaagccgctg aagccctgcc cgccggcacc accgaacgga tcaaatccgg
     5221 ccccgccgac
//
LOCUS       DATBRX010000143        18370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1948090, whole genome shotgun sequence.
ACCESSION   DATBRX010000143 DATBRX010000000
VERSION     DATBRX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 18370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18370
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948090"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1290)
                     /locus_tag="VIF08_05460"
     CDS             complement(91..1290)
                     /locus_tag="VIF08_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY8135469.1"
                     /translation="MRTITTAAILAVGSELTTGTTRDTNSGDLARELTGLGVRVLRSV
                     ALPDELAIATEAVADALRDADLVVCTGGLGPTPDDLTREAIAAACGLEPRVDDELLAG
                     IEALFTRRGIAMPDANRKQAWLIDGATALPNAHGSAPGWWVDRPDGRIIIALPGPPRE
                     MWPMWREHALPRLHAGALGVERATHTLRLTGVGESALVALIGEDVLRAANPQVATYAR
                     ADAVDVVVSADGDGRLTARSIVDAAVAKLRTQVGEFVFAEGDQTWADALAARLGGRTL
                     ATVELGTAGHLQALLGSAPYLRFGELVADEANLHDAATDLGHFAQRVREVGGANIGVA
                     LFARQTKDTHVRIAIATDHGVEEMTRVAFLAGDEGRRRAALACAAALWQFLGRPHPSP
                     GDRRSRR"
     gene            complement(1287..1694)
                     /locus_tag="VIF08_05465"
     CDS             complement(1287..1694)
                     /locus_tag="VIF08_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8135470.1"
                     /translation="MTGERSKQHREFFAVPLDGAALEDVRWQPVAGQSEWISELILAD
                     NLDQRTKTGSRTRLVRWQPGTLLPEPSRHDYREEILLLSGDLVVGCDAHGSGGEQFAA
                     LTFATRPAGIWHGPFTTRTGCVMFELDIYESRQ"
     gene            1829..3466
                     /gene="groL"
                     /locus_tag="VIF08_05470"
     CDS             1829..3466
                     /gene="groL"
                     /locus_tag="VIF08_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8135471.1"
                     /translation="MAKLITYDEDARAAMKRGMDALADAVKTTLGPKGRNVALDRRYG
                     APTVTHDGVTVAKDIELDDPFENMGAQLLKEAATKTEDVAGDGTTTATVLAQAIVTDG
                     LRNVASGANPMQMKLGIDKAAKAVSDAIRAAATPVKGRDEIAQVATVSAADKEIGNLI
                     ADVMDKVGKDGVITVEESKSMQFETEYVEGMQFDRGFTSAYFVTDTTRMEAALDDPYI
                     LITDKKISAIPDLLPTLEKILTSGKKDILIIAEDVDGEALSTLVVNKLRGVLNVLAVK
                     APGFGDRRKEMLRDIAILTGGTVISEELGRKLDSVVVEDLGRVRRVVANKDNTTLIEG
                     RGNEADIAARIKQIKAQIEDTTSDYDKEKLQERMAKLSGGVAIIKVGAGTETELKEKK
                     HRVEDALSATRSAVEEGIVPGGGVALINAQAALDSLKLDGDAAVGVSIVRSALEEPMR
                     QLAENAGMNGQVVLENVRRSQAAKKSTTVGFNVMTEEYTDLVAAGIVDPAKVTRSAVE
                     NAASIGGMVLTTEVMITDKPEPKPAMPQMPPGGMGDF"
     gene            3538..4350
                     /locus_tag="VIF08_05475"
     CDS             3538..4350
                     /locus_tag="VIF08_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135472.1"
                     /translation="MSEERAPGLGAQFGRTRAAFVGLFQAHVDLLKAEIAEILSQLKL
                     ITAQAGCALVILMMTGTLLYVGGFLFVGEWLFGSIGWGLAHGVLFGVAMAVILGMAII
                     GATRGAALGSLFLAVLVTVGLALLCGSNAAYNAARDIATRMAFPLDSTVSVALLAGAV
                     IGAIVFALMLARLAGRTGFIGGLVLGTILGMPIAWLIAAAPWTWPPAVGFAIALGLIL
                     WPILYVVLALPGLDPAERFAKLAPRQSIEAAKETRAWLEEQWQTRRQKLGGR"
     gene            4314..4922
                     /locus_tag="VIF08_05480"
     CDS             4314..4922
                     /locus_tag="VIF08_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135473.1"
                     /translation="MADETTEARRPVAPPPPKVAEALKGVRVARRGVATELDNLNTSV
                     RAAVNIPAKIKRNPVPTLGAAGGAAFLLLGGPRRVAQSVEKRLFPKRYNRPPTVLPRD
                     VEKTIDRLPEEDRERVRAHLERDFAAYLREEHTKDPPNARTAAWKTYDLLLGIVGAAA
                     ARELVKKLFAVPKEVRVEETKETAEVEVEVEEKKAKARSKAG"
     gene            complement(4919..5785)
                     /gene="arsM"
                     /locus_tag="VIF08_05485"
     CDS             complement(4919..5785)
                     /gene="arsM"
                     /locus_tag="VIF08_05485"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEY8135474.1"
                     /translation="MTTQTVAPATTDPTAEAIHEEVRARYAAAAREADKGCCAVDSDS
                     YGAELYSALEKAELPNAAVLASLGCGNPIAVADLHEGERVLDLGSGGGIDVLLSAKRV
                     GPTGRAIGLDMTDEMLALAQRNAAQAGATNVEFVRGHIEDIPLPAESVDVVISNCVIN
                     LAADKPAVFREIARVLRPGGRLGFTDIVAEDRLSPADRAERGSYVGCIAGALSYSEFV
                     QGLESVGLTDISLTATHAVADGLDSAIIKATKPLAAGTTAAGPVIDVSRRELPLAKTS
                     TGSCGCGEGGCC"
     gene            5835..6173
                     /locus_tag="VIF08_05490"
     CDS             5835..6173
                     /locus_tag="VIF08_05490"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8135475.1"
                     /translation="MKRPTDPDHLLLNGAADPTRLEILRQLAAADGVCACDFTACCEV
                     AQPTVSHHLKVLRESGWVRAERRGSFVYYFLRPDAVARFQELARQISPAGLGVPVPAA
                     RILPVIQPLA"
     gene            complement(6103..6972)
                     /locus_tag="VIF08_05495"
     CDS             complement(6103..6972)
                     /locus_tag="VIF08_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135476.1"
                     /translation="MPSTQPSPRLVMNATIAALIANLAAALPTLLIGDILTGPAAMNG
                     SARGTALIMVVLGVPVAAIALWFERRGSWRAPFVLFGAFLYFAYNNFLLLTATPFNPL
                     FLVYTTSMSLTLLVIALRSIAADPRALADRLSQLPVRAIAVFAWVVVGFNVLAWLAKI
                     VPATFAAVPTSYLEGTGLTTNPVFVQDMSFWLPFGALIAWLLWTRRAWGVFLGGAWLV
                     YGFIEGIGVATDQYLGASADPTSTQVSFAIVPMFLALAALTAVPLYLYFRRAAESPAK
                     FARLGPARQGQPG"
     gene            complement(7075..7320)
                     /locus_tag="VIF08_05500"
     CDS             complement(7075..7320)
                     /locus_tag="VIF08_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135477.1"
                     /translation="MIAVEPPRLFRTRFGNRILRGENQALFEPDGNGTWLTQEFWTKG
                     LVSAVMARVFATGSYKGSFQGELNEFARIASAEGRLA"
     gene            7546..7629
                     /locus_tag="VIF08_05505"
     tRNA            7546..7629
                     /locus_tag="VIF08_05505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7579..7581,aa:Leu,seq:tag)
     gene            7662..8171
                     /locus_tag="VIF08_05510"
     CDS             7662..8171
                     /locus_tag="VIF08_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8135478.1"
                     /translation="MPRRKNRRYSKDLLEPLVAHAFSVAEVLRMLNLRQAGGNHLNIS
                     RRIRQYGLDTSHFRGSAHNRGQIGKRKAAAEILVERPDMANRSHAMHLRRALVEIGLP
                     VRCALCGVSDQWRGAPLVLEIDHINGRHNDNRAENLRFLCPNCHSQTETFCARNIGVS
                     EEYAYYGAA"
     gene            complement(8168..9454)
                     /locus_tag="VIF08_05515"
     CDS             complement(8168..9454)
                     /locus_tag="VIF08_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY8135479.1"
                     /translation="MTAAPSGAAGRQVPGPLRFGRRARFAAVLAVLGPGIVSGFADND
                     AGGITTYSLAGAKFGYDLLWVILVTQLALFVTQEIGARMGLATGQGLTGLIRERFGVR
                     WAAFAALTMILANLGSTVSEFAGISAALSLFGVPVPVSAVLAAVAVITLISWGSFGRV
                     QYAFVAIGLLVSIAYIVSAALARPDWSLAAASLVVPHGTFSGAYMLAVVGTVGTTITP
                     WGQGFIQAYVVDKRLRPEELPAARVDVFIGVLITNVVAAFIVIACAATLFATGQTDIT
                     DAAQAAQALGPIAGPTASTLFAVGLLAASLLGLGVVPLASAYTACEAFGWEQGVGWRV
                     REAPAFYGLLAFFIGFAALFMLIPGLPLIQVMFSAQVLNGLLLPVILVFVMLLAGDKR
                     LMGNLASGRINLALGWTVTVMLVVMSLVLVVTAFSS"
     gene            complement(9451..10716)
                     /locus_tag="VIF08_05520"
     CDS             complement(9451..10716)
                     /locus_tag="VIF08_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8135480.1"
                     /translation="MIYLSQVIGQPVRDQNGEAIGKVADLIVAVGERYPPVTGLVINT
                     DRRQIFLPWSSVAAVDVSGATLSTSQLDITKFSQRPNEILLKLDLLDKQIVDIDGRRV
                     VRVNDVSLDEVEGSLRLVAVDVGTAGLLRRLGIERPYRRIARGLGRSTGERYIDWEDV
                     DPLESTIAAVKLRVPHAGLAELHPADLATIIDQLTPRDRAGVLASLDDEAVADAIEEL
                     EPESQVDVLEELQPDRAADILEEMSPDDAADLVADLSDTARAEILALMEKTEADEVKE
                     LLGYPEETAGGIMTTEYIAVPATLTAAQTIDKLRALEPSAETIYYVYVVDDDGRLVGV
                     LSLRDLIVAKPEMAINEVMIDEPVAVGVLDHQREVANLVARYNLLAVPVVDESGRLEG
                     IVTVDDTLDSLLPVGWKSHLPRRFPGSRR"
     gene            complement(10729..11433)
                     /locus_tag="VIF08_05525"
     CDS             complement(10729..11433)
                     /locus_tag="VIF08_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135481.1"
                     /translation="MGGFLRFVAWFVLLVAGFVLVGLPLLVGPLLTGTVRDMGVRADT
                     LNVSVALFDPGLVLGRSRQVTIDATNVDMAPATIGSFHLGLGNVDYFERGFESISGDL
                     TDVRLVMGNDSVSAASATVAGTADAATVTAGFAADQVAQLLTITAARNGLTLQDVTVT
                     PSGVRVTLHGVEADARLLVRGGALLLDPGVGGPQVLLQPEPSDPWRLTDVWFSEGGMN
                     LSGTVDVADIARNLAH"
     gene            11456..12868
                     /gene="tig"
                     /locus_tag="VIF08_05530"
     CDS             11456..12868
                     /gene="tig"
                     /locus_tag="VIF08_05530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8135482.1"
                     /translation="MINAETGPKKVTTRPAERSSVVLEVEFPATEVQRSVAESVRHLA
                     RSTRVPGFRPGKAPRALIERSLGINRDDPAAPDPLYDDAKEHLFETSVLEAARDQNLD
                     LLAVPAPEWLTFSEAGGASYRVVLPIRPEVKLGAYTDYPFGITIDEITDAEVDKVIDQ
                     LRDQQASLVPVEGRGAAAEDYAVIRFSGSRDGTPIEGAQAERMPLIIGRERFVPGFED
                     QIVGLSEGEGKSFSLTFPADYPEADLASKPVDFDVTLLELRSKKMPDADDDFARSLGA
                     YSDLATLRTEIRARLERNALDRARHVFSDRIIEFAVANATVELPDLLVERELEIMLDE
                     LRVRLAEQGIAYDEYLRVTERDEAKVLGEFKPDAERRVKTLLVLSEIAQKENVEVTDT
                     ELDTEVSRSRERYAGNPKLVAYLESARGKTYTRSLLRRAKLVEMLVDRWIDQHPEFKN
                     VQHLHEDGHSHDSAVEEEPR"
     gene            12868..13548
                     /locus_tag="VIF08_05535"
     CDS             12868..13548
                     /locus_tag="VIF08_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8135483.1"
                     /translation="MLVPMVIETSARGERAYDIYSRLLRDRIIFLGDVIEDHMANLII
                     AQLLFLEAEDPDRDISLYINSPGGVVTAGLAIYDTMQYLRAPVSTICIGMAASMASVL
                     LAAGAKGKRYALPNSRIMIHQGSGGFRGNTPDVFIQVKELERLNQINHEIMAKHTGQP
                     LEKIIRDTERDYFMAPTDAKTYGIIDEVYTAPDQSLISEARHKGLLGGEGAAAAADEE
                     SGPKPAKG"
     gene            13582..14883
                     /gene="clpX"
                     /locus_tag="VIF08_05540"
     CDS             13582..14883
                     /gene="clpX"
                     /locus_tag="VIF08_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY8135484.1"
                     /translation="MATSSGKGTKVPYRCSFCGKSQEQVRKLIAGQGVYICDECINLC
                     QEIIDEEMLEAPTRAKPSAARLPNPRQIMNALDQYVISQDRAKKVLAVAVYNHYKRVA
                     AGNVVDEVELQKSNVLLVGPTGSGKTLLAQTLARVLDVPFCIADATSLTEAGYVGEDV
                     ENILLRLIQAADFDVARAQRGIIYIDEIDKIARKSDNPSITRDVSGEGVQQGLLKILE
                     GTVANVPPQGGRKHPHQDFIQIDTTNILFICGGAFEGLEKIVEERVGKRTMGFGSQAQ
                     VDKPQREKVLSQMMPEDLLKFGLIPEFVGRLPVIVSLMALTRDDLMRILIEPKNAVTR
                     QFQKFLQLDKVELTFTPGAIEAAAELALNRKTGARALRSIVEESLLEVMFDIPERTDV
                     RKCIITEETIRHGRAPLLLTKAEVERGVDETNYEELIERPA"
     gene            14886..15350
                     /locus_tag="VIF08_05545"
     CDS             14886..15350
                     /locus_tag="VIF08_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8135485.1"
                     /translation="MADPVIEATRVVLEESLAEIRKGVVDLSVDELNARPAGGNTNSV
                     AVICAHALGSTRSWLSLAFGLPQPPRDRDAEFATVADGAFKQWTEGQIAGIFALLDGA
                     TWDPDRSGVPAWNNRLGAQPRTAPYSLGHALAHLGEHVGHLHMTRELLRPES"
     gene            15354..16274
                     /locus_tag="VIF08_05550"
     CDS             15354..16274
                     /locus_tag="VIF08_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY8135486.1"
                     /translation="MTGLGGFGGRRLAALLGKVGIWSFALQRLAARDEIDAARQFEAQ
                     RFPTLWIPESLGSKDVMAHAALLLGGTDKVTLASGIANIHARDPMAMANGARALGEAF
                     PGRFVLGIGVSHAPSVQMRGGDYSNPLAQMKAYLDAMDEAQYAAPAPDPPVPLVLAAL
                     GPKMLELAAQRADGAHPYFVPVEHTPVARQHLGPDACLATEVTAVLSTDRSAGLDIAR
                     AFAKHYLALPNYANNLRRLGWADADIENDGTDRLLEAVVAIGDVDAIVERVNEHLAAG
                     ADHVCVQIRAEKSTDPAIGAYQELAAAFNL"
     gene            complement(16291..17061)
                     /locus_tag="VIF08_05555"
     CDS             complement(16291..17061)
                     /locus_tag="VIF08_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8135487.1"
                     /translation="MCFDEDSHPPIPPIAGAAVDGRRITLQSADGTDFAAFAAQPAQP
                     NGAAMIVLPDVRGLFTFYEELALRFAEAGVAALAIDYFGRTAGTAPQPAGFDYNPHIS
                     KVTWQGLRADIAAARREIGTWPGVRGVFSVGFCFGGRLSLLLASEKELAMAGVIGFYG
                     WPVGESRGGVPAPIDRLSANGAPILAIFGGADEGLPAEVIESYRSALAGAGAPHEVVT
                     YPGAPHSFFDRKADAYAADSADAWRRVLEFVQANTPTD"
     gene            17351..>18370
                     /locus_tag="VIF08_05560"
     CDS             17351..>18370
                     /locus_tag="VIF08_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY8135488.1"
                     /translation="MATQPGLARPQTTDMAPAYQPGDVEPAIYQRWLDADVFGPEGAG
                     TRADWSKPPFVITQPPPNITGSLHTGHALGTTIQDLMVRRARMQGQPTLWVPGVDHAS
                     ISAQVVLDKIIAKDGETRNSLGRERYLERMWQFISETRELIGNQHQRLGASVDWSRLR
                     FTMDEGSARAVRVGFKKLYDDGLAYRGEKLINWCPRDLTSVSDLEVIATPTKGTLWSV
                     RYHLVDDAGAPIPAETITVATTRPETILGDTAVAVHPDDSRYTALVGRRVLIPFVDRV
                     VPIIADDVVRQDFGTGAVKITPAHDQDDFETGKRHGLPIIDVMTDDGHINENGASFAG
                     LTLNDA"
BASE COUNT         3239 a         6212 c         6180 g         2739 t
ORIGIN      
        1 cagctggggc atggtaccca tgccatgagg aaggggactg gccagtgcaa ccagccgact
       61 gctcgcgctt gtcgtgatcg gggagctagt tcaccgtcgt gagcggcgat caccagggga
      121 tggatgcggc cggcccagga attgccagag ggccgcggcg caggcgagcg cggcgcggcg
      181 gcggccttcg tcgcctgcca ggaaagcgac acgcgtcatc tcttcgacac cgtggtccgt
      241 cgcgatggcg atgcggacgt gcgtgtcctt cgtttggcgc gcgaagagcg ccacaccaat
      301 gttggcgcca cccacctcgc gcactcgctg ggcgaagtgg ccgaggtcgg tcgcagcgtc
      361 gtggaggttc gcctcgtcgg ccacgagctc gccgaatctc aggtacggcg cgctgccgag
      421 cagtgcctgc agatgtccgg cggtgcccag ctcgaccgtc gccagggttc gtccgcccag
      481 tcgggcggcc aaggcgtcgg cccaggtctg gtcgccctca gcgaagacga actcgccaac
      541 ctgtgtgcgt agcttcgcca ccgccgcgtc gacaattgac cgggcggtca gtcggccgtc
      601 tccatccgcg ctgacgacga cgtcgaccgc gtccgcccgg gcgtaagtcg ccacctgcgg
      661 attggcggcc cgcagcacgt cctcgccgat cagcgcgacc agcgccgact cgcccacgcc
      721 ggtcagacgc agagtgtggg tcgcgcgctc cacgcccagc gcgcccgcgt gcaagcgggg
      781 gagggcgtgc tcgcgccaca tcggccacat ctctcggggc ggcccgggca gggcgatgat
      841 gattcggcca tccggacggt ccacccacca gcctggcgcg gagccatgcg cattgggcag
      901 agcagtggcg ccgtcgatca gccacgcctg cttgcggttg gcgtcgggca tcgcgatgcc
      961 gcgccgcgtg aacagtgcct cgatcccggc gagcaattca tcatcgacgc gcggctcgag
     1021 cccgcacgcg gcggcgatcg cctcgcgtgt caggtcgtcg ggcgtgggac ccaggccgcc
     1081 tgtgcagacg acaagatccg catcgcgcag ggcgtccgcg accgcctcgg tcgcgatggc
     1141 cagttcatca ggcagcgcga cgctgcgcag gacgcgcacg cccagtccgg tgagttcgcg
     1201 cgccaggtcg ccgctgttgg tgtcgcgcgt cgtaccggtg gtcagctcac tccccacggc
     1261 caggatcgcg gccgtcgtga tggtcctcac tggcgcgact cgtagatatc gagctcgaac
     1321 atcacgcagc ccgttcgcgt ggtgaatggg ccgtgccaga tcccggcagg ccgcgtcgca
     1381 aaggtgagcg ccgcgaactg ctctccgccg cttccatgtg cgtcgcagcc caccacgaga
     1441 tcgccactca gcagcagaat ctcttcgcgg taatcgtgcc tggagggttc aggcagcaac
     1501 gtgcctggct gccatcgcac cagacgagtg cgcgacccgg tcttcgttcg ctggtcgagg
     1561 ttgtcggcca ggatcagttc ggagatccac tccgattggc cggcaaccgg ctgccagcgc
     1621 acgtcctcga gcgcggcgcc atcgaggggc acggcgaaga actcgcggtg ctgcttcgag
     1681 cgttctccgg tcatcacgcg agttgtagca ctcacccgtt agcaatctca aggccagagt
     1741 gctaacctag agcccgcgcc gcacgattga cgcggccgcg ctgcagcacg cgacaacgga
     1801 acaccctgag agaggacgcc caaccgacat ggccaagctg atcacctatg acgaggacgc
     1861 ccgcgcagcg atgaagcgcg gcatggacgc gcttgccgac gcagtcaaga cgacgctcgg
     1921 gcccaagggc cgcaacgtcg cgctcgaccg gaggtacggc gcccccacag tgacccacga
     1981 cggcgtcacg gtcgccaagg acatcgagct cgacgacccg ttcgagaaca tgggtgcgca
     2041 gctgctcaag gaagccgcga ccaagaccga ggacgtggcg ggcgacggca cgaccacggc
     2101 caccgttctc gcccaggcga tcgtgaccga cggtctgcgg aacgttgcgt ccggcgccaa
     2161 tccgatgcag atgaagctcg gcatcgacaa ggcggccaag gccgtgtcgg atgccatcag
     2221 ggctgccgcg acgccggtca agggccgcga tgagatcgcc caggtcgcca ccgtgtccgc
     2281 ggctgacaag gagatcggca atctcatcgc cgacgtcatg gacaaggtcg gcaaggacgg
     2341 cgtcatcacc gtcgaagagt cgaagtcgat gcagttcgag accgagtacg tcgagggcat
     2401 gcagttcgac cgcggcttca cgagcgccta cttcgtgacg gacacgacgc gcatggaagc
     2461 agcgctcgac gatccctaca tcctgatcac cgacaagaag atcagcgcca tcccggacct
     2521 gctgccgacg ctcgagaaga tcctgacgag cggcaagaag gacatcctga tcatcgccga
     2581 ggacgttgac ggcgaagccc tctcgacgct cgtcgtcaac aagctgcgcg gcgtcttgaa
     2641 cgtcctcgcg gtcaaggcgc cgggctttgg cgatcggcgc aaggagatgc tgcgcgacat
     2701 cgccatcctg accggcggca cggtcatcag cgaagagctc gggcgcaagc tcgactcggt
     2761 cgtggttgag gacctgggcc gcgtccggcg cgtcgttgcc aacaaggaca acacgacgct
     2821 catcgagggc cgcggcaacg aggcggacat cgcggcgcgc atcaagcaga tcaaggcgca
     2881 gatcgaggac accacctcgg actacgacaa ggagaagctc caggagcgca tggccaagct
     2941 ctccggtggc gtggcgatca tcaaggtcgg cgccggcacg gaaacagagc tcaaggagaa
     3001 gaagcaccgc gtcgaggacg cgctctccgc gacgcgctca gcggtcgagg agggcatcgt
     3061 gcccggtggc ggtgtggcgc tcatcaacgc ccaggccgcg ctcgactcac tcaagctcga
     3121 cggcgatgcc gcggtgggcg tgagcatcgt tcgctccgcg ctcgaggagc ccatgcgcca
     3181 gctggccgag aacgccggca tgaacggcca ggttgtgctc gagaacgtgc gccgcagcca
     3241 ggctgccaag aagagcacga cggtcggctt caacgtcatg accgaggagt acaccgacct
     3301 ggtggcggcg ggcatcgtcg atccggcgaa ggtcacccgc tcggcggtag agaatgccgc
     3361 ctcgatcggc ggcatggtgc tcacgacgga ggtcatgatc accgacaagc ccgagcccaa
     3421 gccggccatg ccccagatgc cgccgggcgg catgggcgac ttctaggcat tcggtatgcg
     3481 ccgcaggcca tgcggcgctt gaccgatctg catccccacc cgagcattca cagcgtcatg
     3541 agtgaggagc gcgcgcccgg gctgggcgcc cagttcggcc gcacgcgcgc cgcgttcgtc
     3601 ggcctgttcc aggcgcacgt cgacctgctc aaggccgaga tcgcggagat cctgtcgcag
     3661 ctcaagctga taacggccca ggccggctgc gcgctggtca tcctgatgat gacgggcacc
     3721 ctgctctacg tcggtgggtt cctgttcgtg ggcgaatggc tcttcgggtc aatcggctgg
     3781 ggcctcgccc acggcgtgct gttcggcgtg gcgatggccg tcatcctggg catggcgatc
     3841 attggtgcga cgcgtggcgc cgcgctcggc agcctcttcc tggccgtgct ggtgaccgtc
     3901 ggtctcgcgt tgctatgcgg gtcgaatgct gcctacaacg cagcccggga catcgcgacg
     3961 cggatggcgt tcccgctcga cagcaccgtg tccgtggcac tgctcgccgg agcggtcatc
     4021 ggcgccatcg tgttcgccct catgctggcc cgcctggctg gccgaaccgg gttcatcggc
     4081 ggcctggttc ttggcaccat cctgggcatg cccatcgcct ggctgatcgc cgccgcgccc
     4141 tggacgtggc cgccggcggt cggtttcgcc atcgccctgg gactgatcct gtggccgatc
     4201 ctgtacgtgg tgctcgcgct gcccgggctg gacccggccg agcgcttcgc gaagctcgcg
     4261 ccacgccagt cgatcgaggc cgcgaaagag acgcgcgcat ggttggagga gcaatggcag
     4321 acgagacgac agaagctcgg cggccggtag ccccgccccc gcccaaggtt gccgaggcgc
     4381 tcaagggcgt ccgcgtggcc cgcagaggtg tcgcgaccga gcttgacaac ctcaacacct
     4441 ccgtgcgcgc cgccgtgaac atcccggcca agatcaagcg caacccggtg cccacgctgg
     4501 gcgcggccgg cggcgcggcc ttcctgctgc tgggcggccc caggagagtc gcgcagagcg
     4561 tcgagaagcg cctcttcccc aagcgctaca acaggccgcc cacggtgctg ccgcgcgacg
     4621 tggaaaagac gatcgacaga ctgcccgagg aagaccgcga gcgcgtacgt gcccacctcg
     4681 aaagggactt cgccgcgtac ctgcgcgagg agcacaccaa ggatccgccc aatgcccgga
     4741 ccgcggcctg gaagacttac gacctgctgc tcggcatcgt cggggcggcc gccgcgcgcg
     4801 agctcgtcaa gaaactgttc gcggtgccca aggaagtacg cgtggaagag acaaaggaaa
     4861 ccgccgaggt agaggtcgag gtcgaggaga agaaggccaa ggcgcgctca aaagccggct
     4921 agcagcagcc gccctcgccg cacccgcacg aaccggtcga agtcttcgca agcggcagtt
     4981 cccgtcggct gacatcgatc acggggccag cggcggtcgt gcccgctgcc agtggcttgg
     5041 tcgccttgat gatggcactg tcgagcccat cggcaaccgc gtgggtcgcg gtgagcgaga
     5101 tgtcagtcag gcccaccgat tcaaggccct gcacgaactc cgaataggac agcgcaccgg
     5161 cgatgcagcc gacataggag ccgcgctcgg ctcggtccgc gggggagagg cggtcttcgg
     5221 caacgatgtc ggtgaatccc aggcggccgc ctggtcgcag aacgcgcgcg atctcgcgga
     5281 acaccgccgg cttgtcggcg gcgaggttga tcacgcagtt gctgatgacg acgtcgaccg
     5341 actcggccgg aagggggatg tcctcgatgt ggccgcgcac gaattcgacg ttggttgccc
     5401 ctgcctgggc ggcattgcgc tgggccaggg caagcatctc gtcggtcatg tccaggccga
     5461 tcgcgcgtcc ggttgggccg acgcgcttgg ccgatagaag gacgtcgatc cccccgcctg
     5521 agcccaggtc gagcacgcgc tcgccttcgt ggaggtcagc gacggcaatc gggttgccgc
     5581 agcccaggct ggccagcacg gccgcgttgg gcagctcggc cttctcgagg gcggagtaca
     5641 actcggcgcc gtaggagtcg ctgtcgactg cgcagcagcc cttatcggcc tccctggccg
     5701 cggcggcgta gcgcgcgcgg acctcttcgt ggattgcttc agcggtggga tcggtggtcg
     5761 ccggggcgac ggtttgtgtt gtcatgcgat attgatactt gccaatatcg agacttgtca
     5821 atatgctatt tgtcatgaag cgcccgaccg accccgacca cctgctgctc aacggtgctg
     5881 ccgatcccac ccgcctggag atcctgcgcc agctggctgc cgccgacggc gtgtgcgcgt
     5941 gcgacttcac cgcctgctgc gaggtcgccc agcccaccgt gagccatcat ctgaaggtcc
     6001 tgcgcgagtc cggttgggtg cgcgccgagc ggcgtggctc gttcgtctac tacttcctcc
     6061 ggcccgacgc ggtcgcgcgc ttccaggagc tcgcccgcca gatcagcccg gctggccttg
     6121 gcgtgccggt cccagccgcg cgaattttgc cggtgattca gccgctcgcc tgaagtaaag
     6181 gtagagcggc acggccgtaa gcgccgcaag cgccaggaac atgggaacga tggcgaagga
     6241 cacctgggta gaggtcggat ccgctgacgc tccgaggtac tggtcggtcg cgaccccgat
     6301 gccctcgatg aagccgtaga cgagccacgc cccgcccagg aacacgcccc acgcccggcg
     6361 cgtccacagg agccaggcga tcagggcacc gaatggcagc cagaacgaca tgtcctggac
     6421 gaacaccggg ttggtcgtca ggccggtgcc ctcgaggtag ctggtcggga cagccgcgaa
     6481 cgtggccggc acgattttgg ccagccacgc caggacgttg aagccgacga cgacccacgc
     6541 aaacaccgcg atggcgcgca cgggcagctg cgatagccga tcggcgaggg cacgcgggtc
     6601 agcggcaatc gagcgcagcg cgatgacgag cagagtcagc gacatcgacg tggtgtagac
     6661 caggaacagt gggttgaatg gcgtcgcggt cagtagcagg aagttgttgt aggcgaagta
     6721 cagaaacgcg ccgaagagca cgaacggggc gcgccagctg ccgcgccgct cgaaccacag
     6781 cgcgatcgcg gcgaccggta cgcccaacac caccatgatc agcgcggttc cgcgggcgct
     6841 gccgttcatc gcggccgggc cggtcaggat gtcgccgatc aagagcgtcg gcagggccgc
     6901 ggcgagattg gcgatcaggg cggcaatggt cgcgttcatg acaagtcggg gcgatggctg
     6961 ggtcgagggc atggcgattc tccgaagcgg ctggcgtcac cggaatcgtg ggcttccgcg
     7021 aacccgttgg gacagggccg tccggaccac tggtggacca ccatcaggcc ccagtcaggc
     7081 caggcgcccc tcggctgacg cgatccgcgc gaattcgttc aactcgccct ggaagctgcc
     7141 tttgtacgag ccagtcgcaa acacgcgcgc catcacagcg gacacaagac ctttggtcca
     7201 gaactcctgc gtgagccatg tgccatttcc gtcgggttcg aacagcgcct ggttctcacc
     7261 gcgcaggatg cgattgccaa agcgcgtccg gaacagccgt ggtggctcga ccgcgatcac
     7321 ctccggttgg gctggacatc ttgccgaaca gcgtcctgta gcgcgtccct acctgatcga
     7381 ctggcccggt gatgtcagtc acgccggtga cgccgcatgc ggggcagaag ttcacgggtg
     7441 aatcctagcg gcggtgcccg gccaggtgta ctttgaactc ggggtggacc actcattcct
     7501 gtgggtggcc tcgcaattcg agcggccacc cgtagactca tctgtgggcg agtggtggaa
     7561 cggtagacac accacgttta gggcgtggcg ctgcaaggcg tgtcggttcg aatccgacct
     7621 cgcccaccaa agattagaac agacgtgcta taatggcggc gatgccgcga cgcaagaaca
     7681 ggcgctacag caaggacctg ttggagccac ttgtcgccca tgcattttct gtcgcagagg
     7741 ttcttcggat gttgaacttg cgccaggccg gcggcaacca cctgaacatt tcaaggcgga
     7801 ttcgccagta cggcctcgat acctcgcact tcagaggatc agctcataac cgtggccaga
     7861 ttggcaagcg aaaggccgcg gcggagattt tggtcgagcg acccgacatg gccaatcgat
     7921 ctcacgcgat gcacttgagg cgagcgctgg tcgagatcgg gctccccgtt cgatgtgcct
     7981 tgtgtggagt ctccgatcaa tggcgaggag cgccgcttgt cttggagatc gaccacatca
     8041 acggccgaca taacgacaat cgagccgaga accttcgctt tctttgcccc aactgccatt
     8101 cgcagacaga gacattctgc gcgcggaaca tcggtgtctc cgaggagtac gcgtactacg
     8161 gagctgccta gctgctgaag gccgtcacga ccaacacgag cgacatcacg accagcatga
     8221 cggtgaccgt ccagccgagc gccaggttga tccggccgct ggcgaggttg cccatcagcc
     8281 gcttgtcacc cgcgagcagc atcacgaaca ccaggatcac aggcagcagc aggccgttga
     8341 gcacctgggc gctgaacatg acctggatga gcggcaggcc cgggatcagc atgaacagcg
     8401 ccgcgaagcc gatgaagaac gccagcaggc cgtagaacgc cggcgcctcg cgcacgcgcc
     8461 agcccacgcc ttgctcccaa ccgaaggcct cgcacgcggt gtacgccgag gcgagcggaa
     8521 cgacgcccag acccaacaat gacgcggcca ggagtccaac cgcgaacagc gtcgacgccg
     8581 tgggtccggc gatgggtccg agcgcctgtg cggcctgcgc ggcgtcggtg atgtcggtct
     8641 ggcccgtcgc gaagagcgtc gccgcgcagg cgatgacgat gaacgcggcg accacgttgg
     8701 tgatcaacac tccaatgaag acatcgacac gggccgcggg gagctcctcg ggccgcagcc
     8761 gcttgtcgac cacgtaggcc tggatgaagc cctggcccca cggggtgatc gtcgtgccca
     8821 ccgttcccac cacggcgagc atgtaggcgc cgctgaaagt gccgtgcggc acgaccagcg
     8881 aagccgccgc cagcgaccag tccggccgcg ccagcgcggc cgacacgatg taggcgatcg
     8941 acacgagcag gccgatcgcc acgaatgcgt actgcacgcg cccgaagctg ccccaactga
     9001 tgagcgtgat caccgcgacc gcggccagca cggcgctgac cggcaccggt accccgaaca
     9061 gcgacagcgc cgcgctgatg ccggcgaact cggacaccgt ggagcccagg ttggcgagga
     9121 tcatggtcag cgcggcaaac gccgcccagc gcacgccgaa acgctcgcgg atcagcccgg
     9181 tcagcccctg gccggtcgcg aggcccatcc gggcaccgat ctcctgcgtg acgaacagcg
     9241 ccagctgggt gaccaggatg acccacagga ggtcgtagcc gaacttcgcg ccggcgagcg
     9301 aataggtcgt gatcccgccg gcgtcgttgt cggcaaagcc gctgacgatg cccggtccaa
     9361 ggaccgcgag aaccgccgcg aaacgggccc gccggccaaa gcgcagcggc cccggcacct
     9421 gccttccggc ggcgccgctt ggcgccgcgg tcacctcctg ctaccgggga agcgtcgcgg
     9481 caggtgactc ttccagccca cgggcagcag cgagtcgagc gtgtcgtcga ccgtgacgat
     9541 gccctcaagg cggccgctct cgtcgacgac gggcactgcc aggaggttgt agcgcgccac
     9601 gagattggcc acctcgcgct ggtggtcgag cacgcccacg gccaccggct cgtcgatcat
     9661 gacctcgttg atcgccatct ccggcttggc gacgatcagg tcgcgcagtg acagcacgcc
     9721 gacgagccgc ccgtcatcgt caacgacgta gacgtagtag atcgtttccg ccgacggctc
     9781 cagcgcgcgc agcttgtcga tggtctgggc tgcggtgagg gtggccggca cagcgatgta
     9841 ctcggtggtc atgatgccgc cggccgtctc ctccgggtaa cccagcagct ccttgacctc
     9901 gtcggcctcc gtcttctcca tgagcgccag gatctccgcc cgggcggtgt ccgataggtc
     9961 ggccaccagg tcggccgcat cgtcggggct catctcctcg aggatgtccg cggcgcggtc
    10021 gggctggagc tcctcgagca cgtcgacctg cgactcgggc tccagctcct cgatcgcgtc
    10081 agcgacagcc tcgtcatcga gcgacgccag cacgccggcg cggtcgcgcg gcgtgagctg
    10141 atcgatgatc gtcgcgaggt ccgccggatg gagctcggcg agcccggcgt ggggcacgcg
    10201 cagcttgacc gccgcgatcg tgctctccag cggatcgacg tcctcccagt cgatgtagcg
    10261 ctcgcccgtc gatcggccga ggccgcgggc gatcctgcga tacggacgct cgatgcccaa
    10321 ccggcgcaac agccccgcgg tgcccacgtc cacggcgacc agccggagcg agccctccac
    10381 ctcgtcgaga ctgacgtcgt tgactcgcac gacgcgccgg ccgtcgatgt cgacgatctg
    10441 cttgtcgagc aggtcgagct tcagcaggat ctcgttcggg cgctggctga acttggtgat
    10501 gtcgagctgc gaggtgctca gcgtcgcccc gctcacgtct accgccgcga cagacgacca
    10561 gggcaggaag atctggcggc ggtcggtgtt gatgaccagg ccggtcaccg gcgggtagcg
    10621 ctcaccgacc gcgacgatca ggtcggcgac cttgccgatc gcctcgccgt tctggtcgcg
    10681 aacgggctgg ccgatgacct ggctgaggta gatcacgcgc gtccgagtct agtgcgccag
    10741 gttgcgcgcg atatcggcca catcgaccgt gccggatagg ttcatgccac cctcgctgaa
    10801 ccagacatcg gtcagccgcc acgggtccga cggctccggc tggagaagca cctgggggcc
    10861 gccgacgccg gggtccagca gcaatgcccc gccgcgcacc aggagccgcg cgtcggcctc
    10921 cacgccgtgc agcgtgacgc gcacgccgga gggcgtgacc gtcacatcct ggagcgtcag
    10981 cccgttgcgc gcggccgtga tcgtcagcag ctgcgcgacc tggtccgcgg cgaaacctgc
    11041 ggtgaccgtg gccgcgtccg cagtgcctgc aacagtggcg ctggcggcgc tgacagagtc
    11101 gttgcccatc accaggcgca cgtcggtgag gtcgcccgag atgctctcga agccccgctc
    11161 gaaatagtcc acgttgccca gccccaggtg gaacgagccg atcgtcgccg gcgccatgtc
    11221 gacgttcgtg gcatcgatcg tgacctggcg cgagcgacca aggaccaggc ccggatcgaa
    11281 cagcgccacg ctgacgttca gcgtgtcggc gcgcacgccc atatcacgca cggtgccggt
    11341 cagcagcggc ccgacgagca gcggcaggcc cacgagcacg aagcccgcca cgagcagcac
    11401 gaaccacgcc acgaaacgga ggaaaccgcc cacgcccgta tactcggacc ttccaatgat
    11461 caatgccgag actggtccca agaaggtcac gaccaggccg gccgagcgaa gctctgtcgt
    11521 gctcgaagtc gagtttcccg ccacagaggt ccagcgctcg gtcgccgagt ccgtgcgtca
    11581 cctcgcgcgc agcacgcgcg tgcccggctt ccgcccgggc aaggcgccgc gcgccctgat
    11641 cgaacgctcg ctgggcatca accgcgacga cccggccgcg cctgacccgc tgtatgacga
    11701 tgccaaggag cacctcttcg agacgtccgt gctcgaagcg gcgcgcgacc agaacctcga
    11761 cctgctggcc gtgccggcgc ccgaatggct gacgttcagc gaggccggcg gcgccagcta
    11821 ccgcgtggtg ctgccaattc gcccggaggt aaagctcggg gcctataccg actacccgtt
    11881 cgggataacc atcgacgaga tcaccgatgc cgaggtggac aaggtgatcg accagctgcg
    11941 cgaccagcag gcatcccttg tgccggttga agggcgcggc gccgcggccg aggactacgc
    12001 cgtcatccgc ttcagcggat cgcgcgacgg cacgcccatc gagggcgccc aggcagagcg
    12061 tatgccgctc atcatcggcc gcgagcggtt cgtgcccggt ttcgaggatc agatcgtggg
    12121 tctgtcagag ggggagggca aatcattcag cctgaccttc ccggccgact accccgaggc
    12181 cgatctggcc agcaagccgg tcgacttcga cgtcacgctg ctcgagctgc gttccaagaa
    12241 gatgcccgac gcggacgacg atttcgcgcg ctcactcggt gcgtacagcg acctcgcgac
    12301 gctcaggact gagatccgcg cccgcctgga gcgcaacgcg ctcgaccggg cgcgccacgt
    12361 cttctccgac cgcatcatcg agtttgccgt cgccaacgcc accgtcgagc tgcccgacct
    12421 gctggtcgag cgcgagctcg agatcatgct cgacgagctg cgtgtccgcc tggccgagca
    12481 gggcatcgcc tatgacgaat acctgcgcgt cacggagcgc gacgaggcga aggtcctggg
    12541 cgagttcaag cccgacgccg agcggcgcgt caagacgctg ctcgtgctca gcgagatcgc
    12601 ccagaaggag aacgtcgagg taaccgacac cgagctggac acggaggtca gccgctcgcg
    12661 cgagcgctac gccggcaacc ccaaactcgt cgcgtacctc gaatcggcgc gcggcaagac
    12721 ctacacgcgc tcgctcctgc gccgcgccaa gctcgtcgag atgctcgtcg atcgttggat
    12781 cgaccaacat cccgagttca agaacgtgca acatctacat gaagacggcc attcccatga
    12841 ctcggccgtt gaggaggaac cacgctgatg ctcgtcccga tggtcatcga aacgtccgcc
    12901 cggggtgagc gcgcctacga catctacagc cgcctgctgc gcgaccggat catcttcctg
    12961 ggtgacgtga tcgaagacca catggccaac ctgatcatcg cccagctgct cttcctcgag
    13021 gcggaagacc cggaccgcga catcagcctg tacatcaact cgcccggtgg cgtggtcacg
    13081 gccggcctgg cgatctacga cacgatgcag tacctgcgcg cgccggtctc gacgatctgc
    13141 atcggcatgg ccgcgtcgat ggcgagcgtc ctgctcgccg ccggcgccaa gggcaagcgc
    13201 tacgccctgc cgaacagccg gatcatgatc caccagggct cgggcggctt ccgcggcaac
    13261 acgccggacg tcttcatcca ggtcaaggag ctcgagcggc tcaaccagat caaccatgaa
    13321 atcatggcca agcacaccgg ccagccgctc gaaaagatca tccgcgacac ggagcgggat
    13381 tacttcatgg ccccaacgga cgccaagact tatggcatca tcgacgaggt ctacaccgcg
    13441 cccgatcagt cgctgatcag cgaagcacgc cacaagggtc tccttggcgg cgagggagct
    13501 gcagcggcgg cggacgagga gagtgggccc aagcctgcca agggatgaac tgacgagcgg
    13561 ttgatcggag gcatcggcta aatggctaca tcgtctggca agggcaccaa ggtgccctat
    13621 cgctgcagct tctgcggcaa gagccaggag caggtccgca agctgatcgc cggccagggc
    13681 gtgtacatct gcgacgagtg catcaacctg tgccaggaga tcatcgacga ggagatgctc
    13741 gaggcgccga cgcgcgccaa gccgagcgcc gccaggctcc ccaacccgcg ccagatcatg
    13801 aacgcgcttg atcagtacgt gatcagccag gatcgggcga agaaggtcct cgcggtcgcg
    13861 gtctacaacc actacaagcg cgtcgccgcg ggcaacgtcg tcgacgaggt ggagctccag
    13921 aagtcgaacg tgctgctggt cgggcctacg gggtccggca agacgctgct cgcgcagacg
    13981 ctggcgcgtg tgctcgacgt gccgttctgc atcgccgatg cgacttcgct gacagaggcc
    14041 ggctatgtcg gcgaggacgt cgagaacatc ttgctgcggc tcatccaggc cgccgacttc
    14101 gacgtcgcgc gggcgcagcg cgggatcatc tacatcgacg agatcgacaa gatcgcgcgc
    14161 aagagcgaca acccgtcgat cacgcgcgac gtcagcggcg agggtgtgca gcaggggctg
    14221 ctcaagatcc tcgaggggac ggtcgccaac gtgccgccgc agggcgggcg caagcatccg
    14281 caccaggact tcatccagat cgacaccacg aacatcctgt tcatctgcgg tggcgcgttc
    14341 gagggtctcg agaagatcgt cgaggagcgc gtcggcaagc gaacgatggg cttcggctca
    14401 caggcccagg tcgacaagcc gcagcgcgag aaggtgctct cgcagatgat gccggaggat
    14461 ctgctcaagt tcgggctcat tccggagttc gtcggacggc tgccggtcat cgtgtcactc
    14521 atggcgctga cgcgcgacga cctgatgcgc atcctgatcg agcctaagaa cgcggtgacc
    14581 cgccagttcc agaagttcct gcagctcgac aaggtggagc tcaccttcac gccgggcgcc
    14641 atcgaggccg cggcggagct cgcgctcaac cgcaagaccg gcgcgcgtgc gctgcgctcg
    14701 atcgtcgagg agtcgctgct cgaggtgatg ttcgacatcc ccgagcggac cgatgtgcgc
    14761 aagtgcatca tcaccgagga gaccatccgt cacggtcgcg cccctttgct gctgaccaag
    14821 gctgaggtcg agcgcggcgt cgacgagacg aactacgaag agctgatcga gcgaccggcc
    14881 tgaccgtggc cgatccagtg atcgaggcga cgcgcgtcgt cctcgaggag agcctggccg
    14941 agatccgcaa gggcgtcgtc gatctgtctg tcgatgagct caacgcccgt cccgctggcg
    15001 gcaacaccaa ctccgttgcg gtgatctgcg cccacgcatt gggctcgacg cgctcgtggt
    15061 tgagcctcgc gttcgggctg ccccagccgc cgcgcgatcg cgacgcggaa ttcgcgacag
    15121 tcgcggacgg cgctttcaag cagtggaccg agggacagat cgcgggcatc ttcgccctgc
    15181 tcgatggcgc gacatgggat cccgatcgca gcggcgtccc tgcctggaat aaccgcctgg
    15241 gcgcgcagcc gcggaccgcg ccgtattccc tgggccacgc gctggcgcac ctgggcgagc
    15301 acgtcggcca cctgcacatg acgcgcgagc tgctgcggcc cgagtcctag ggggtgacgg
    15361 gcctcggcgg gtttggtgga cgcaggctcg ccgcgctgct tggaaaagtc ggcatctgga
    15421 gcttcgcgct gcagcggctc gcggcgcgcg acgagatcga tgccgcgcgg caattcgagg
    15481 cgcagcgctt cccgacgttg tggatccccg aaagcctggg cagcaaggac gtcatggccc
    15541 atgcggcgct gctgctgggt ggcacggaca aggtcacgct tgcgagcggg atcgccaaca
    15601 tccacgcgcg cgaccccatg gcgatggcca acggcgctcg ggcgctgggc gaggcgttcc
    15661 caggccgctt cgtcctcggg atcggcgtct cgcacgcgcc gtcggtgcag atgcgcggtg
    15721 gcgattacag caatccgctt gcccagatga aggcctacct tgatgcgatg gacgaggcgc
    15781 agtacgcagc gcccgcgccg gacccgccgg tgccgctcgt gctggccgcg ctcgggccga
    15841 agatgctcga gcttgccgcg cagcgcgcgg acggcgcgca tccctacttc gtgcccgtgg
    15901 agcacactcc tgtggcgcgc cagcacctgg gccccgacgc gtgtctcgcg acggaggtca
    15961 cggcggtcct ttcgaccgac cgatcagccg gcctcgatat cgcgcgcgct ttcgccaagc
    16021 attacctggc attgcccaac tacgccaaca acctgcgccg cctgggctgg gctgacgccg
    16081 atatcgagaa cgacggcacc gaccggctgc tcgaggcggt ggtggccatc ggcgacgtcg
    16141 atgcgatcgt cgagcgcgtg aacgagcatc tcgccgcggg tgccgatcac gtctgcgtgc
    16201 agatccgagc ggagaagtcg accgacccgg ccatcggtgc ctaccaggag ctcgcagccg
    16261 ccttcaacct ctgagacacc atcgccacgc ctagtccgtt ggcgtgttcg cctgtacgaa
    16321 ctcgaggacg cgccgccagg catcggctga gtcggctgcg taggcgtcgg ccttacggtc
    16381 gaagaacgag tgcggcgcgc ccgggtaggt gacgacctcg tgcggcgcgc cagcacctgc
    16441 cagggcgctg cggtacgact cgatgacttc cgccggcagc ccttcatccg ccccgccgaa
    16501 gatagccagg atgggtgcgc cgttggccga caaacggtcg atcggcgccg ggacgccgcc
    16561 gcgcgactcg cccaccggcc agccgtagaa gccgatcacg ccggccatag ccagctcctt
    16621 ctcggaagcg agcagcagcg agaggcggcc gccgaagcag aagccaacgc tgaacacgcc
    16681 gcgcacccct ggccaggtgc cgatctcgcg ccgcgcggca gcgatgtcgg cgcgcaggcc
    16741 ctgccaggtc accttggaga tgtgcgggtt gtaatcgaag cccgcgggct gcggcgcggt
    16801 gccggccgtg cgaccgaagt agtcgatcgc cagggccgcg acgccggcct cggcgaagcg
    16861 cagcgccagc tcctcgtaga acgtgaacag gccgcgcacg tcgggcaaca cgatcatcgc
    16921 tgcgccgttg ggctgggcgg gctgagcggc gaacgcggcg aagtcggtcc cgtcggccga
    16981 ctggagggtg atccgcctgc cgtccacggc agcgccggca atcgggggaa tcggtgggtg
    17041 gctgtcctcg tcgaagcaca tgcgggggat gctaacccgg ctggtagact cacggctcgt
    17101 tccagcgacc ggaaggagta gcgccattcc ggcctgatca gagaagtccg gccgcaggct
    17161 gagagccgga ccaggcacca tggcgcgaac gtcgtccgcg aggagcgcgg gcccgcccgt
    17221 tagcgcggca agagggtgtt ggagaaccgc tcgcgggatg accgcggtac ggccagcacc
    17281 gaaccagcag gtggtaccac gaccccgttc gtccgctgga cgatcggggc gttttgattt
    17341 tggagacacc ttggccacac agcccggtct cgcacggccg cagacaaccg atatggcgcc
    17401 ggcttatcag cccggtgacg tcgagccagc gatctaccag cgctggctgg atgcggatgt
    17461 cttcggtccg gaaggggcgg gcacccgggc cgactggtcc aagccgcctt tcgtgatcac
    17521 gcagccgccg cccaacatca ccggttccct ccacaccgga cacgcactcg ggaccaccat
    17581 ccaggatttg atggtgcgcc gcgcgcggat gcagggccag ccgacgcttt gggtgccggg
    17641 cgtcgaccac gcgtcgatca gcgcccaggt ggtgctcgac aagatcatcg ccaaggacgg
    17701 tgagacgcgc aactcgctcg gccgcgagcg ctatctcgaa cgtatgtggc agttcatcag
    17761 cgagacgcgc gagctgatcg gcaatcagca ccagcggctg ggggcgtcag tcgactggag
    17821 ccggctgcgc ttcaccatgg acgagggctc ggcgcgcgcg gtgcgcgtcg gcttcaagaa
    17881 gctgtacgac gacggtctcg cctaccgcgg cgagaagctc atcaactggt gcccgcgcga
    17941 cctgacgagc gtgtccgatc tcgaggtcat cgcgacgccg accaagggca cgctgtggag
    18001 cgtgcgctac cacctcgtgg atgacgctgg cgctccgatc ccggcagaga cgatcacggt
    18061 ggccacgacg cgcccggaga cgattctggg tgacaccgcc gtcgcagtgc acccggatga
    18121 ctcgcggtac acagcgctgg tcggtcggag ggtgctcatc ccgtttgtgg acagggtcgt
    18181 gccgatcatc gccgacgacg tcgtgcgcca ggactttggc accggcgccg tcaagatcac
    18241 gcccgcccat gaccaggacg acttcgagac cggtaagcgc cacggcctgc cgatcatcga
    18301 cgtgatgacc gacgacggcc acatcaacga gaacggtgcg tccttcgccg ggctgacgct
    18361 caacgacgcg
//
LOCUS       DATBRX010000144         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3736659, whole genome shotgun sequence.
ACCESSION   DATBRX010000144 DATBRX010000000
VERSION     DATBRX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3736659"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VIF08_05565"
     CDS             <1..610
                     /locus_tag="VIF08_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8135489.1"
                     /translation="DRVRSAGGESEAFRADLTDGHQAEALIQHAIDRFGRVDALVNNA
                     GRTQVGPFLEISPDEWNGVIATDLSAAFHTCRAVLPSMVERGSGAIVNVCSRLGQMGI
                     AETAAYSAAKSGLIGLTRSLAREFGPSGIRINAVAPGPIATDMTEDLLASDEGRRRLR
                     DLPLGRFGRADEVANAVIFLLSDASSLFLGQTINPNSGGYMP"
     gene            618..2171
                     /locus_tag="VIF08_05570"
     CDS             618..2171
                     /locus_tag="VIF08_05570"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8135490.1"
                     /translation="MKVISVREAVELVKDGQTFVVGGSGAGHALPQRFIDELESVFKE
                     TGHPRDLTSVRVVGTGDFADRGFSQLALPGMHRRTIGSNIGNEPRLGAMVENNELEAY
                     SLPQGVLSQLMREIAAGRPGLLTHVGLGTYVDPRQTGGKQNARTTEDLVEVVNVRGRE
                     WLLYHAFPIDVAVIRGTTADEDGNITMEGEAIQAEMLAMAMAARNSGGIVICQVRQVA
                     PRGSLPQRDVKVPGALVNYVYVDPDQWQTYITKDSPYYAGKERKPVGGDPPLPLDVRK
                     IIARRSLLEFKPGFICNLGFGISQLIGRVAWEEGITDQLVLTVEQGIFGGVPVAGNEG
                     GAGFNYQAMIDQPDMFGFYDGGGLDIASLSFAEVDAQGNVNVHAFEGRIRGPGGFPNI
                     SARTPRINFVGTLTAQGLELDISTAGVRVKTEGTLPKFVERVREVTFNGELARQRGQQ
                     VRYITDRAVFALDDDGIVLTEVAPGIDVERDVLAQMQFRPRVAEGLRSIDPRVYAGGP
                     MGLAADFGR"
     gene            2168..>2363
                     /locus_tag="VIF08_05575"
     CDS             2168..>2363
                     /locus_tag="VIF08_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135491.1"
                     /translation="MSLVNLTLGQVAQLELDNAPMNLMSDEMLAAFEAALASLEAAAP
                     GDVRAVVVSSAVERAFSAGSD"
BASE COUNT          384 a          772 c          825 g          382 t
ORIGIN      
        1 ggatcgcgtg cgaagtgcgg gcggagagtc ggaggctttt cgggccgacc tgaccgatgg
       61 ccaccaggcg gaggccctca tccagcacgc catcgatcgc ttcggccgcg tcgatgcgct
      121 cgtcaacaac gcgggtcgca cgcaggtcgg gccgttcctc gagatcagcc ccgatgagtg
      181 gaacggcgtc atcgcgaccg atctcagcgc tgctttccat acctgccggg cggtgctgcc
      241 gtcgatggtt gagcgcggta gcggcgcgat cgtcaacgtt tgctcgcgcc tgggccagat
      301 gggtatcgcc gagaccgccg cgtactcagc cgcgaagtcc gggcttatcg gcctgacacg
      361 ctctctggcc cgcgaattcg gaccgagtgg catccgcatc aacgccgtcg cacctggacc
      421 gatcgccacg gacatgacgg aggatctgct cgcgagtgac gagggccggc gccgcttgcg
      481 cgacctgccg ctgggccgct ttggccgcgc cgacgaagtc gcgaacgcgg tgatcttcct
      541 gctgtcggac gcgtcatcgc tgttcctggg ccagacgatc aatcccaaca gcggcggcta
      601 catgccatga gcgccgcatg aaggtcattt cggtccgcga ggcagtcgag ctggtcaagg
      661 acggccagac attcgtggtc ggcggatcgg gcgcgggtca cgcgctgccg cagcgcttca
      721 tcgacgagct cgagtcggtc ttcaaggaga ccggccatcc gcgtgatctg acatcagtgc
      781 gggtggtcgg taccggcgac ttcgccgacc gtggcttcag tcagctggcc ttgccgggga
      841 tgcaccggcg caccatcggc agcaacatcg gcaacgagcc ccgactgggc gcgatggtgg
      901 agaacaacga gctggaggcg tactcgctgc cccagggcgt gctctctcag ctgatgcgcg
      961 agatcgcggc cgggcgacca gggctgctga cgcacgtggg cctgggcaca tacgtcgatc
     1021 cgcgccagac cggtggcaag cagaacgcgc gtacgacaga ggacctggtc gaggtggtca
     1081 atgtgcgcgg ccgcgaatgg ctgctctatc acgcgttccc gatcgacgtc gcagtcatcc
     1141 gtggcacgac cgccgatgag gacggcaaca tcacgatgga aggcgaggcg atccaggcgg
     1201 agatgctcgc catggccatg gccgcacgga attcgggtgg gatcgtcatc tgtcaggtgc
     1261 gccaggtggc gccgcgcggc agcctcccgc agcgcgacgt gaaggtgcca ggtgcgctcg
     1321 tgaactacgt gtacgtcgac cccgaccagt ggcagaccta catcaccaag gattcgccct
     1381 actacgccgg aaaggagcgc aagcccgtcg gcggcgatcc accgctgccg ctcgacgtgc
     1441 gcaagatcat cgcccgccgc tcactgctcg agttcaagcc gggcttcatc tgcaacctgg
     1501 gcttcggcat ctcgcagctc atcggccgcg tcgcctggga ggagggcatc acggaccaac
     1561 tggtgctgac cgtcgagcag ggcatcttcg gcggcgtgcc cgtcgccggt aacgaaggtg
     1621 gggccggctt caactaccag gcgatgatcg accagcctga catgttcggc ttctacgacg
     1681 gcggtgggct cgacatcgcg tcgctctctt tcgcggaggt cgacgcccag ggcaacgtca
     1741 acgtccacgc cttcgagggg cgtatccgcg ggccgggcgg ctttccgaac atcagcgcgc
     1801 gtacgccgcg catcaacttc gttggcacgc tgacggcgca gggcctggag ctggacattt
     1861 ccactgcggg agtccgcgtc aagacggagg ggacgctccc caagttcgtg gagcgcgtcc
     1921 gcgaggtgac gttcaacggc gagctggcgc gccagcgcgg ccagcaggtg cgctacatca
     1981 ccgaccgtgc cgtgttcgcg ctcgatgacg atggcatcgt gctgacggaa gtcgcgccgg
     2041 gcatcgacgt cgagcgcgac gtgctggccc agatgcaatt ccggccgcgc gtcgccgagg
     2101 ggctgcggtc gatcgatccg cgcgtgtacg caggcgggcc gatgggcctc gcggcggact
     2161 tcggccgatg agcctcgtca acctcacgct gggccaggtc gcgcagctcg agctggacaa
     2221 cgcgccgatg aatctcatga gcgacgagat gctcgcggcg ttcgaggccg cgctggcgtc
     2281 gctcgaagcg gcggcgccgg gtgacgtccg cgcggtcgtc gtcagcagtg ccgttgagcg
     2341 ggctttctcg gccggatcgg acg
//
LOCUS       DATBRX010000145         4840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_944349, whole genome shotgun sequence.
ACCESSION   DATBRX010000145 DATBRX010000000
VERSION     DATBRX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944349"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VIF08_05580"
     CDS             complement(<1..668)
                     /locus_tag="VIF08_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8135492.1"
                     /translation="MVAGQADRHAAELAGRLGRALKDARVLRGMTQARAAERAGLAQT
                     TWSKLERDLDPRFTLATWDRAAHAVGTTLSAYLPQASSADQPRDAVHLRNQELALRKA
                     KPGGWRGAPEQALDRDLTRSRHGDLVLSRHRAGASEWCLIEVVDWVSDVGESVRDFDR
                     RLAALDRFAVARMRADAVPRSGGIWLLRATARNRRLVADHHEFFRGRFPGSGRAWLAA
                     LTDPA"
     gene            complement(879..1715)
                     /locus_tag="VIF08_05585"
     CDS             complement(879..1715)
                     /locus_tag="VIF08_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEY8135493.1"
                     /translation="MEITTTRGAGAALDTMPVCPECGATMVVRAQWHNSHVQGLNWVC
                     RRAPGCEGVRRIRNPDEIRPINHDASTQAIFDWESSRDARVSRVPRRDARGATESRLG
                     GFLGRLVSRPTERASDYDEPAAVHGSMGYFDGLVELGFVVLEDRHLPHARVVMDNVLV
                     GPSGVFVVERKAWPGHLAVAADSVYVDGRARMGATDAILRATDAFEQTMAHELKPVGA
                     TVRPAILFENATNKTVEGAADKVLVGGTRGLPKLLRGTAEPVLGPETIVRLALAADRL
                     LE"
     gene            complement(1778..2299)
                     /locus_tag="VIF08_05590"
     CDS             complement(1778..2299)
                     /locus_tag="VIF08_05590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8135494.1"
                     /translation="MTREEMRDKIQVAMDYLNEHPREARYTDDAAVARLTDPTTLKVD
                     VTGPAGAHITTDMPTSVGGSNSATSPGWYLRAAEASCVATLIAMRAAHQGIQVENIEV
                     TVDSQSDDRGILGADLYTPAGPLSTRVAVRLRAVGADRQHLQKVVDWAVEHCPVTDAV
                     RRAIPMNVELEIS"
     gene            complement(2296..3075)
                     /locus_tag="VIF08_05595"
     CDS             complement(2296..3075)
                     /locus_tag="VIF08_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8135495.1"
                     /translation="MDLGLKGARALVTGGSAGLGAAVARSLTEEGARVAIAARAGEKL
                     TATATDLSALAIPTDLATTDGPASAVATAAAQLGGLDALLISMGGPPPGKFSELTDEA
                     WQKALDMTFWSTLRVLRAALPLLTDSANAGRAGSICIILSSSVREPIPALATSNLLRP
                     GLAGLLKSLVAEIAPVRINGIAPGRVATDRITQLDNDRATRSGEPVEEIQRQTIARIP
                     LGRYGEPAEVGRVATFLLSPAASYVTGVIVGVDGGMIKSLP"
     gene            3097..4023
                     /locus_tag="VIF08_05600"
     CDS             3097..4023
                     /locus_tag="VIF08_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY8135496.1"
                     /translation="MRRLLLWMASNPWMRRNIPRLWFAKRAVRKFMPGESAADALAAA
                     EKFRDELGIATLFTRLGENVTRAEQHEEAANHYNWLIEEIHRRGIPGEASVKLTHLAM
                     DMSDADMARHMNRLCETAAANGNQVVWVDMEGSDYTDKTLAEYSRLKTLYPNVGICLQ
                     AYLHRTAADVEALLPLAPRIRLVKGAYKEPREIAWQSRREVDASYLALCVAMLTHKKD
                     GRDIFLGMGTHDVKLIEQVAAAASSLGLARDSFDVEMLYGIRADQQKRLKAAGYNVRV
                     LIAYGDYWFPWYMRRMAERPANVLFALRQILP"
     gene            4020..>4840
                     /locus_tag="VIF08_05605"
     CDS             4020..>4840
                     /locus_tag="VIF08_05605"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8135497.1"
                     /translation="MTDELARRTDQLQGVWNIVPTPFHPDEELDLPSLRTLTDFVVGS
                     GVDGMTILGVLGEGAKVSDRERSAIVEVTLAQANGRVPVCVTVSAASAYRSIGYAREA
                     RAMGAHSVMLSAPPLARPNDDAVRRNFLRVAESVPELPVVIQDLPALGVWMSAELIGR
                     MGSEAPNLRVVKLEEEPTAPKVARLLAASGELRVLGGLGAEMLIEELRSGAVGTMTGF
                     GYPETLVAIVKSWRAGDEAAAVELFHRWTPLIRFENQQLLNLPIRKHIYMRRGAIA"
BASE COUNT          749 a         1654 c         1709 g          728 t
ORIGIN      
        1 gccgggtctg tcagcgccgc gagccacgcg cggcccgaac ccgggaagcg accgcggaag
       61 aactcgtgat ggtccgcaac cagtcggcgg ttgcgcgccg tcgcgcggag tagccagatg
      121 ccgcccgatc gtggcacggc atcggcgcgc attcgcgcca cggcgaagcg atccagggcc
      181 gccagcctcc gatcgaaatc ccggacggat tcgccaacgt cgctgaccca atcaacgacc
      241 tcgatcaggc accactccga cgcgcccgcg cgatgccgac tcagcaccag atcgccatgt
      301 cgcgagcgtg tcagatcgcg gtcgagcgcc tgttccggcg ctccccgcca gccacccggc
      361 ttcgcctttc gcagcgccag ctcctggttt cgcaggtgca cggcgtcgcg cggctggtcg
      421 gccgagctcg cctgcggcaa gtacgcgctc agcgtggtcc cgaccgcgtg cgcggcgcgg
      481 tcccaggtcg cgagggtgaa cctcgggtcg agatcgcgtt cgagcttgct ccaggtggtc
      541 tgcgcgaggc ccgcgcgctc agcggctcgc gcttgcgtca tgccgcgcaa gacccgggca
      601 tccttcagcg ccctgcccag acgaccggcg agctcggcgg catgccgatc agcctgaccg
      661 gcaaccatcg cccgtctatg tcgtcccata cccgcactat ggttgcgggg agcacttact
      721 cggcgcttat cggccgaatc ggccccccat aggcgtcatc caagcgcgcg tgtgcgtgcc
      781 cgatggggtg gggaacccag tgtctggggc ttccgccccc gcgcagtaat agtgcggtta
      841 tgcagggcgg gaaggagccg cgaggggcag acggcgcgct actccagcag gcggtccgcc
      901 gcaagcgcca ggcggacgat ggtctctggg ccgaggacgg gctcggcggt gccgcgcagc
      961 agcttgggca agccgcgcgt gccgccgacg agcaccttgt cggccgcgcc ctcgacggtc
     1021 ttgttggtcg cgttctcaaa gaggatcgcc ggccgcaccg tggcgccgac gggtttgagc
     1081 tcgtgggcca tggtctgctc gaaggcgtcg gtggcgcgca ggatcgcgtc ggtcgcaccc
     1141 atgcgcgcgc ggccgtcgac atacacggag tcggccgcga ccgcgaggtg gccgggccac
     1201 gccttgcgct cgacgacgaa gacgccggag ggtccaacca ggacgttgtc catcacaacg
     1261 cgcgcgtggg gcaagtggcg gtcctccagg accacgaagc ccagctccac caggccgtcg
     1321 aagtagccca tcgagccgtg gaccgcagcc ggctcgtcgt agtcggaggc ccgctcagtc
     1381 ggccgggaga caagcctgcc aaggaagcca cccagcctcg actcggtcgc gccccgggca
     1441 tcgcgccgcg gcacgcggga cacgcgggca tcacgcgagc tctcccagtc gaagatggcc
     1501 tgcgtgctcg cgtcgtggtt gatcggccgg atctcgtcgg ggttgcggat gcggcgcacg
     1561 ccctcgcaac ccggggcgcg ccggcaaacc cagttcagcc cctggacgtg gctgttgtgc
     1621 cactgcgcac ggacgaccat ggtcgcgccg cactcggggc acacgggcat ggtgtcgagc
     1681 gcagcaccag ccccccgggt ggtcgtgatc tccataggga ccgactaggg tagacccgac
     1741 cgccgagcgg cgtctagtgg gggccaagcc ccgcatatca gcttatttcg agctcgacgt
     1801 tcatcgggat cgcgcggcga acggcatccg tgaccgggca gtgctcgact gcccaatcga
     1861 cgaccttctg aaggtgttga cggtcggcac cgaccgcccg cagtcggacc gccacgcggg
     1921 tgctcagcgg ccccgcgggg gtgtagaggt cggcgcccag gatgccgcgg tcgtcagatt
     1981 gcgagtcgac cgtgacctcg atgttctcga cctggatgcc ctggtgcgcg gcgcgcatcg
     2041 cgatcagggt cgcgacgcac gatgcctcag ccgcgcgcag gtaccagccc gggctcgtgg
     2101 cggagttgct gccgccgacc gatgttggca tatcggtcgt gatgtgggcg ccggccggcc
     2161 ccgtgacgtc gaccttcaac gtcgtgggat ccgtcaggcg cgcgaccgcg gcatcgtcgg
     2221 tgtagcgcgc ctcccgggga tgctcgttga ggtagtccat cgcgacctgg atcttgtcgc
     2281 gcatctcttc tctggtcatg gcaacgactt gatcatgccg ccgtcgacgc cgacgatcac
     2341 gccggtgacg taagacgcgg caggggagag caggaaggtc gccacgcggc cgacctccgc
     2401 gggctcgccg tacctgccga gcgggatgcg cgcgatggtc tggcgctgga tctcctcgac
     2461 cggttcgccg ctgcgtgtcg cacggtcgtt gtcgagctgc gtgatgcgat cggtcgcgac
     2521 gcggcccggg gcgatgccgt tgatgcgcac cggcgcgatc tcggcgacga gcgacttgag
     2581 aaggccggcg aggccgggcc ggagcaggtt cgaggtcgcc agcgcgggga tgggctcgcg
     2641 cacgctcgac gacaggatga tgcagatcga gccggcccgg ccggcgttgg cactgtcggt
     2701 cagcagcggc agggctgcgc gcagcacgcg cagcgtcgac cagaaggtca tgtcgagcgc
     2761 cttctgccac gcctcgtcgg tgagctcgct gaacttgccg gggggtggcc cgcccatgct
     2821 gataagcagc gcatcgagcc cgcccagctg tgccgcggcg gtggccacgg ctgaggccgg
     2881 tccatcggta gtggccaaat cggtcgggat cgccagggcg gatagatcgg tggcagtcgc
     2941 cgtcagcttc tcgcctgctc tcgccgcgat ggcgacgcgc gccccttcct cggtgagcga
     3001 ccgcgccacg gccgcgccca gtcccgcgct gccgcccgtt acgagcgccc gcgcgccctt
     3061 cagtccaagg tccacccgct aacgttagcg gcacccatgc gccgactgct gctctggatg
     3121 gcaagcaacc cctggatgcg ccgcaacatc ccgcggctgt ggttcgccaa acgcgccgtg
     3181 cgcaagttca tgcccggtga atcggctgcg gatgccctgg ccgcggcaga gaagttccgc
     3241 gacgagctcg ggatcgcgac gctcttcaca cgactgggcg agaacgtcac ccgcgccgag
     3301 cagcacgagg aggccgcgaa tcactacaac tggctgatcg aagagatcca ccgccgcggc
     3361 atccccggtg aggcgagcgt caagctgacg cacctggcga tggacatgtc ggacgcggac
     3421 atggcgcgcc acatgaaccg gctgtgcgag acggcggcgg ccaacggcaa ccaggtcgtg
     3481 tgggttgaca tggaaggctc cgattacacc gacaagacgc tggcggaata cagccgactg
     3541 aaaacgttgt atcccaacgt agggatctgc ctgcaggcct accttcaccg caccgcggcc
     3601 gatgtcgagg cgctactgcc gctggcgccc aggatccgcc tggtcaaggg cgcgtacaag
     3661 gagccgcgcg agatcgcgtg gcagtcgcgg cgcgaggtcg acgcctcgta cctggcgctg
     3721 tgcgtcgcga tgctgaccca caagaaagac ggccgcgaca tcttcctggg catgggcacg
     3781 cacgacgtga agctgatcga gcaagtcgcg gctgccgcct cgtctctggg cttggcgcgc
     3841 gactcgttcg atgtcgagat gctctacggc atccgcgccg accagcagaa gcggctcaag
     3901 gccgcgggct acaacgtgcg cgtcctgatc gcgtacggcg actactggtt cccgtggtac
     3961 atgcgccgca tggccgagcg ccccgcgaac gtgctgttcg cgctgcgcca gatcctgccg
     4021 tgacggacga gctggcgcgg cgcactgatc aactgcaggg cgtatggaac atcgtcccga
     4081 cgcccttcca ccctgacgaa gagctggacc tgcccagcct gcgcacgctg accgatttcg
     4141 tggtcgggag tggcgtcgac ggcatgacga tcctcggcgt gctgggggag ggcgccaagg
     4201 tgtcggatcg cgagcgatcg gccatcgtcg aggtcacgct tgcccaggcg aacgggcgcg
     4261 tgccggtgtg cgtgacggtc agcgcagctt cggcttatcg ctcaattgga tacgcgcgcg
     4321 aggcgcgagc gatgggcgcg cacagcgtca tgttgtcggc gccaccgctg gcgcggccga
     4381 atgacgacgc cgtgcggcgc aacttcctgc gcgtggcgga atccgtgccg gagctgccgg
     4441 tcgtcatcca ggacctgccc gcgctcgggg tgtggatgag cgccgagctc atcgggcgga
     4501 tggggagcga ggcacccaac ctgcgcgtgg tcaagctcga ggaagagccc acggccccca
     4561 aggtcgcgcg gctgctcgcg gccagcgggg agctgcgggt gctgggcggc ctgggcgctg
     4621 agatgctgat cgaggaactg cgcagcggcg ccgtcggcac gatgaccggc ttcggctacc
     4681 cggagacgct cgtggccatc gtgaagtcgt ggcgcgccgg ggacgaagcc gcggccgtgg
     4741 agctgttcca ccgctggacg ccactcatcc ggttcgaaaa ccagcagctg ctcaacttgc
     4801 ccatccgcaa gcacatctac atgcgtcgcg gcgcgatcgc
//
LOCUS       DATBRX010000146        11956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3625796, whole genome shotgun sequence.
ACCESSION   DATBRX010000146 DATBRX010000000
VERSION     DATBRX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625796"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /gene="groEL"
                     /locus_tag="VIF08_05610"
     CDS             <1..890
                     /gene="groEL"
                     /locus_tag="VIF08_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8135498.1"
                     /translation="IVAEDVDGEALATLVVNKLKGTISVLAVKAPGFGDRRKEMLRDM
                     AILSGAQVISEELGRKLDSVTAEDFGSARRVVATKDDTTIVDGGGKPDEIKGRMTQIK
                     AQVEETTSDYDREKLQERLAKLAGGVAVIKVGAATEVELKEKKHRIEDALSTTRAAVE
                     EGIVAGGGTALLQAIPALDSLTLEGDEAIGVNIVRRALEAPVRQIAENAGQEGSVIVS
                     AVRGQKIGEGYDALRGEYGNMFDKGIVDAAKVTRSALQHAASIAGMVLTTETLVTDKP
                     EKKESSGAGGHGHGDDMDY"
     gene            1432..1698
                     /locus_tag="VIF08_05615"
     CDS             1432..1698
                     /locus_tag="VIF08_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135499.1"
                     /translation="MHPLVFERYGEGQCRDYVSGFEADPKTVWTVMSSSGPDPWAWET
                     DGLTTTVTDAWTVVVEIPDNGQRDVHFAPFEGRWRWFVDCGDPR"
     gene            complement(1842..2057)
                     /locus_tag="VIF08_05620"
     CDS             complement(1842..2057)
                     /locus_tag="VIF08_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135500.1"
                     /translation="MIQRKKRHPIMGLFFGLLLGLGLGLMAIIYGIYFAGPMTPWLLV
                     GLGVVIGLVMSFVPRPWGRKPPPAGTR"
     gene            complement(2070..2720)
                     /locus_tag="VIF08_05625"
     CDS             complement(2070..2720)
                     /locus_tag="VIF08_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135501.1"
                     /translation="MRRVISAGAVAATLALILVLPVLAAQLSGGCSMEVRSFAGPGAT
                     GTEIDNAAVNGPIPEGAVGSQSRPFKIDPAGSIDFAFHTGTSVFSDNHWTIYAQGLPV
                     PILSGFDDNPSDVDETGIVEIDKTVQKLPFKFVGTVFVSGDLWGNGDTLHCYGEGYVQ
                     VLGDPVGTIPWDIAAALILLSGVMLLVATPYSTTWETDPDSGESLHSGPVTNDPAG"
     gene            2851..5610
                     /locus_tag="VIF08_05630"
     CDS             2851..5610
                     /locus_tag="VIF08_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8135502.1"
                     /translation="MKQAPDRPLEPDLPPLPDAPPGEDLEPAEETPDPRARARSRAAA
                     GPAAIDDAPHAADFFSPVASAAARPADGPEAESAAADREALAASIVTRLNPEQARAVT
                     TTDGPLLILAGAGSGKTRVLAHRVAYLIGVRNVKPWQILAVTFTNKAAAEMRARILAL
                     VGERARDVAMGTFHALCARVLRRDGSAIGIDPRFTIYDTDDQTSTMKLVMRNLELVGS
                     SELKPQAILGQIGRWKNDLLNPEQAAEAARGYHEEVAARAYERYQARLREQGALDFDD
                     LLNEAVRLFEQAPGVLAHYHDRWRYLHVDEYQDTNRAQYLWIRLLAGTRKNVAVVGDD
                     DQSIYSWRGADLRNILDFERDYPNATVVKLEQNYRSTQLILDAAHAVVSRNEARKDKK
                     LWTENPRGVQIERFEADSEDEEAEWIARQVEALVGGRGAGGSFLARRADEGDERRYSL
                     RDIAVMYRTNAQSRAIEEAFLRYGLRYQLVGGTRFYQRREVKDALAYLRVLRSDYDAA
                     AYERIINVPARGIGEKTIALIREEAAKRAGNVWVALVELSRREDGGSRTRSAIAAFVD
                     MVTSLRQRVGVLDLPDLLDDVLEKSGYRAMLADGSQEGEDRWANLLELREVVTRYGDL
                     DPDDAVDRLLEETALVADQDSYEKDSDAVTLITLHAAKGLEFDAVFISGLEEGVFPHS
                     RALDDQRQMEEERRLAYVGLTRARHRLYLTHAATRATWGRGGFSIPSRFLLEIPSDLM
                     HGPRLVAPDEGFTDDDQRPVDERNGYDLSTILGPRGGSRLVGRRGQWGGAGATGNGGG
                     ALTPGRRILPPGGGYTPPVGAPREGESFRPSRDLAAKRAAYYGEADAPPAMAARQVAP
                     QRPVVPGERRYRDGDRVRHAAFGEGTVVTSKLTRDDEEVTVAFPGAGIKKLLASFANL
                     ELLDG"
     gene            5700..9548
                     /locus_tag="VIF08_05635"
     CDS             5700..9548
                     /locus_tag="VIF08_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135503.1"
                     /translation="MPVPSAAGKPWEGMDWRDEGTPEWRDSLSEEQADEWRNWQYDSQ
                     QEAAGGGPAGGGARGEPAGEDYEPPTDSSYQPGSGGPDAQSWDYDSLPDGAREPAVDS
                     APSEPELPSEPPDPTGKPWEGMDPLGDFSTEFRLSLSDAEADDFAQWRDWHEQKAEEE
                     ERAQDEQEQRERDERLRREQEEEKRTGQVDQYRLDHGAPAPPGKAVAFIDDQGNPVDA
                     VVKDQPIKIQVTLPRTEGQDPPRTIDVVVSGPRLTFIGREYSGTDSVTLEWIGNVDGP
                     AIYRSGSMTVENGGEGVSSLDVGGFEFNTGGGMGGFSIDDGDFVQVEIEGVTNGIQVF
                     TSVAKLEIALTLTAINTSHQSYTNVLINLDSLIEQVKAAPEGKEKADLLQQLTFTRDK
                     AQQVVTLANKAIHHFNYVDALDHQHLAVLRLYATWMGLFREPTEGPDGKKTTEFGPLF
                     VPDPRDENDFVKDESNAAKEEVRRIFWAGLTQATIGGYRLLAQATMVAQIMTIFGTNE
                     MGQRASGMERWMAFFDLLSQAALMGAMMKWNTSMATGTGLPTGIVPRTTGRIPRQKPL
                     TDTATAKSAPSGTPVAPEAAGMLRHGARDGQLVALKNDAVIQVRFTSPDAMPLRAQGF
                     TPKSPLVKSKTIKEIDRQIGAPDAAQNGAAGFFEPKLPERGTMTDAQWAKVQERFDLR
                     QGEYDGPIGQYQRKLADEGKLQLRDGMVCDKDGTPFAGDYDLFEITNRDGTPVSAEKY
                     DAVVDDLMNAPGFQAAHGAHMRWNPRPTDPAYHLDMSGDTQLALDAVAKWQADRGIFR
                     DIVAQHQPGGEGLITFAPNQPPVITYAGEAVGPKFADLGITPIAPSAGTGPTVFVTPR
                     LFVATTQQLLDGRFPDGAEWEWTRAWDWQSEVADWQQKADALDTGAITVTADDAPIVD
                     VTRSVSAIGAPLPLTIQPRSGGFNWKPIAIGGVLLLAVAGGGFVLLNQPGPGPGIALG
                     SPTNAPLSTQPTQPPTQPPTTPPTAPPLTAPPLNPSLTAVNTVIGEWSGGRVELPPLW
                     TVGRDHLADDSDDLYLPNDGSAATAIQQHVDMTGVFALVADFTAADAVGLSLNPCAGT
                     FESPGGFTFNTACNQNVPLAAGQYLVVVTTWAQAMPDPFPETAQCSISVQSDVDNDPT
                     TGFQSNLPFNYLIGADVYYENLVFVSNAGEYQNYILATDYSLPPRSDTGEIHGNKPFA
                     GRVIWGSDAGTPAVGATQTFLIPLDEVGDSFAVGGFCTTDRGRIGPDTLAVDAIGAPE
                     DDDFPYYLDVADVFFPPRVP"
     gene            9599..11686
                     /gene="ligA"
                     /locus_tag="VIF08_05640"
     CDS             9599..11686
                     /gene="ligA"
                     /locus_tag="VIF08_05640"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY8135504.1"
                     /translation="MTPQKAAARVAELREQIERALKQYHELDAPEITDAEYDALYREL
                     VELEEAHPELHTPDSPTQRVGGAPSGALAEVRHSTPMLSLNNAFSFDELRAFDARARR
                     LLDLAEEDAARTLRYECELKIDGLAISLRYVRGRFVQGATRGDGTTGEDVTANLRTIE
                     SVPKVLTEPVSIEVRGEIYMPKAEFARINAEREEAGLPLYANPRNSGAGSLRQIDPQV
                     TASRRLAAWFYVLVEAGSATTQTAALDRLEKLGFPVEPNRSTTDLDMDGVTAFIEHWQ
                     EPRHDLAYETDGVVVKVNRFDLQRSLGMVSHAPRWAIAYKYPPEQVETVIEDIVAYVG
                     RTGTLTPVARMTPTKVAGSTVANATLHNLDEVRRKDIRVGDHVVLHKAGDVIPEVVRV
                     ILEKRPKGAKEWQMPATCPVCGTAIVRDENAVRHYCPNLACPARVGQELGHFVGRGSM
                     DIEGAGWKVLVQLIERGLVKTRGDFFRLSVEDIESLDRFARKSAENLHASIQKARVRP
                     LYRIIHGLGIPQVGVTTAIELSNWLAARWPPREDEPMGGQDGWFKRIATELRTVPATD
                     FEQIPGVGPTVAAALERWLTDPLTADVLDDLVDAGVEPERPRAQAAANTEGPLSGKTV
                     VVTGTLKGFDREQAEQAIRAAGGKPGGSVSKKTNYLVAGDSAGSKLAKAQELGVAVID
                     EEGFRKVLAGEEP"
     gene            11690..>11956
                     /locus_tag="VIF08_05645"
     CDS             11690..>11956
                     /locus_tag="VIF08_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8135505.1"
                     /translation="MDLHHLADVLAEQSAGGRAYLEFLRSDSLSVGLYVLRVGDVDRQ
                     QPHREDEIYVVMSGRSRFTAGEDTRQVTAGDTIFVAAGVPHRFHD"
BASE COUNT         2125 a         4031 c         4030 g         1770 t
ORIGIN      
        1 tgatcgtcgc cgaggatgtc gatggcgagg cactcgcgac gctggtcgtc aacaagctga
       61 agggcactat ctccgtgctc gccgtcaagg cccccggctt tggcgaccgg cgcaaggaga
      121 tgctgcgcga catggccatc ctctcgggtg cccaggtcat cagcgaggag ctcgggcgca
      181 agctcgactc cgtcaccgct gaggacttcg gcagcgcccg acgcgtggtc gcgaccaagg
      241 acgacacgac catcgtcgat ggtggcggca agccggacga gatcaagggc cgcatgaccc
      301 agatcaaggc gcaggtcgag gagaccacgt ccgactacga ccgcgagaag ctccaggagc
      361 gcctggccaa gctcgccggt ggtgtcgccg tgatcaaggt cggtgccgcg acagaggtcg
      421 agctcaagga gaagaagcac cgcatcgagg acgcgctctc gaccacccgc gcggcggtcg
      481 aggaaggcat cgtcgccggt ggcggtacgg ctctactgca ggccatcccg gcactcgact
      541 cactgacgct cgagggcgat gaggcgattg gcgtcaacat cgttcgccgc gcgctggagg
      601 cgcctgttcg ccagatcgcg gagaacgccg gccaggaagg ctcagtcatc gtttcggcgg
      661 tgcgtggcca gaagatcggc gagggctacg acgcgctcag gggcgagtac ggcaacatgt
      721 tcgacaaggg catcgttgat gccgccaagg tgacgcgttc ggcactccag cacgcggctt
      781 cgatcgcggg catggtgctg accacggaga ccctcgttac cgacaagccc gagaagaagg
      841 agtcctcagg cgctggtggc cacggccacg gcgacgacat ggactactag gtcgcgcgtt
      901 tcacacacgc agtccgagcc cgggccgagc aatcggcccc ggtctcctgt ttggcttaat
      961 acggactacg gcgtgaggcg ccgtcaatct tgattgcacc gcgaccctcc attagggttt
     1021 cgcggcgggg ggccccgttc catcagcggg agtgctgcgt tgaacttaaa ccgctggttg
     1081 cagtttggcg ccgtactcgt tgtggccttc cttttgagtg ttggcgccgg cgtctggctg
     1141 ggccgccagc ggactgggcc accggtggct aacgtgccca caccgagccc ggctgcgacc
     1201 gcgctctcca cggccagctt gccgccaacg ccttcgccga cgctgaccac gtcgccgact
     1261 gtcgccccga cgttcacccc gagcccaggc ccgccgacag tcccgccgac gctgccgccc
     1321 agcccaagcc cagcatcacc tccggcgctc gatcccccca cggctgagga ttttgctgac
     1381 gcgcttcaag ctctcttccg gtctggcgac acgcagtatc tggccgatcg tttgcacccc
     1441 ctggtcttcg agcgttacgg cgagggtcag tgccgcgact acgtgagtgg cttcgaggcg
     1501 gacccgaaga ccgtctggac cgtcatgtca tcgagtggcc cggacccttg ggcgtgggag
     1561 acggacggcc tgacgacaac cgtgaccgat gcctggaccg tcgtggtcga gattccggac
     1621 aacggccaac gcgacgttca cttcgcgcca ttcgagggga gatggcgctg gttcgttgac
     1681 tgcggcgatc cgcgctagga ggtgaagcga tgactgagcc gactgccccg accagtttcg
     1741 gctattgcgt tgtcttcggc acgccatcgg tcggactgct cacatagaag aggcggagcg
     1801 gtggcccgct ccgcctctgt ggtcgcctcg cgacagacgg tctagcgcgt gcccgccggc
     1861 ggtggcttgc ggccccaggg ccgcggtacg aacgacataa caaggccgat taccacgccc
     1921 aggccgacca gcagccaggg tgtcatcggg ccggcgaaat agatgccgta gatgatggcc
     1981 atgagcccca ggcccagtcc gagcagcagc ccgaaaaaga ggcccatgat ggggtggcgc
     2041 ttcttccgct gaatcattgc tgcgctctcc tagcctgcgg ggtcgttggt caccgggccg
     2101 ctgtggagcg actcgcccga gtccgggtcg gtttcccagg tcgtgctgta cggcgtagcc
     2161 accagcaaca tgacgcctga caagaggatc agcgcagcgg cgatatccca cgggatggtc
     2221 ccgacggggt cgcccagcac ctgcacatag ccctcgccgt agcaatggag cgtgtccccg
     2281 ttgccccaca ggtcgccgct cacgaacacc gtgccgacga acttgaaggg cagcttctgg
     2341 accgtcttgt cgatctcgac gatgcccgtc tcgtcgacgt cgctcgggtt gtcgtcgaag
     2401 cccgaaagaa taggcaccgg cagaccctgg gcgtagatcg tccagtggtt gtcggagaag
     2461 acgctcgtgc ctgtgtggaa cgcgaagtcg atgctgcctg cggggtcaat cttgaacggc
     2521 cgcgactgag agccgacagc gccttccgga atcgggccat tcacggccgc gttatcgatc
     2581 tcagttcccg tggcaccagg tccggcgaat gaacggacct ccatgctgca gccgccgctg
     2641 agctgcgccg ccaggaccgg caggaccagg attagggcca gcgtcgcggc gacggcaccg
     2701 gccgagatca cccgtctcat gtgtcctcca ccctcgtccg agctcgtcgg ctcggcaggc
     2761 ccgcacgata gcaaagacga cactccgcaa acgaccgaaa acaggcttgc ggaactggca
     2821 gataggcggc cgctggtagc atcggccgga gtgaaacagg cgcccgacag acccttggaa
     2881 cccgatctgc cgccgctgcc ggacgctccc cccggagagg atctcgaacc ggccgaagag
     2941 acgccggatc cccgggccag ggcgcgctcg cgggccgcgg ccggccccgc cgcgatcgac
     3001 gatgcgccgc atgccgccga cttcttttcg ccggtggcca gcgccgcggc acgaccagcc
     3061 gacggacctg aagcggagtc ggcagccgcc gatcgcgagg ccctggccgc gagcatcgtc
     3121 acgcgcctca accccgagca ggcccgggcc gtcacgacga ccgatgggcc gctcctcatc
     3181 cttgccggcg cgggcagcgg caagacgcgt gtgctggcgc acagggtggc gtacctgatc
     3241 ggcgtcagga acgtgaagcc gtggcagatc ctggccgtga cgttcaccaa caaggccgcg
     3301 gccgagatgc gcgcacgcat cctggcgctc gtcggggagc gcgcgcgcga cgtcgcgatg
     3361 ggtaccttcc acgccctgtg tgcgcgcgtg ctgcggcgcg acggcagcgc catcggcatc
     3421 gatccgcgct tcaccatcta cgacaccgac gaccagacct cgacgatgaa gctcgtcatg
     3481 cgcaatctcg agctggtcgg ctcgagcgag ctcaaacccc aggcgatcct gggccagatc
     3541 ggccgctgga agaacgacct gctcaacccg gaacaggcgg cagaggcggc gcgcggctac
     3601 cacgaggagg tcgcggccag ggcatacgag cgctaccagg cgcggctgcg cgagcagggc
     3661 gcgctcgact tcgacgatct cctaaacgag gccgtgcggc tgttcgagca ggcacccgga
     3721 gtgctcgccc actaccacga ccgctggcgc tacctgcacg tcgacgaata ccaggacacc
     3781 aaccgcgcgc agtacctgtg gatccgcctg ctcgcgggca cccgcaagaa cgtcgcggtc
     3841 gtgggtgacg acgaccagag catctactcg tggcgcggcg cggacctgcg caacatcctc
     3901 gactttgagc gtgactaccc caacgccacc gtcgtcaagc tcgagcagaa ctaccgctcc
     3961 actcagctga tcctcgacgc cgcgcacgcg gtcgtgtcgc gcaacgaggc gcgcaaggac
     4021 aagaagctct ggacggagaa cccacgcggc gtccagatcg agcgcttcga ggccgattct
     4081 gaggacgagg aggcggaatg gatcgcgcgc caggtcgagg cgctcgtcgg cgggcgcggc
     4141 gctggcggct cattcctggc gcgccgcgcc gacgagggtg acgagcggcg ctactcactg
     4201 cgcgacatcg ctgtcatgta ccgcaccaac gcccagagcc gcgcaatcga ggaggcgttc
     4261 ctgcgctacg gcctgcgcta ccagctcgtg ggaggcacgc gcttctatca gcggcgcgag
     4321 gtcaaggacg cgctcgcata cctgagagtg ctgcgctctg actacgacgc cgccgcgtat
     4381 gagcggatca tcaatgtgcc ggcacgcggc atcggtgaga agacgatcgc gctgatccgt
     4441 gaggaagcgg ccaagcgcgc tggcaacgtc tgggtcgcgc tggtagagct atcgcgccgg
     4501 gaggatggtg gcagtcggac gcggtcggcc atcgccgcct tcgtcgacat ggtcacttcg
     4561 ctccgtcaaa gggtcggcgt gctcgacctg cccgatctgc tcgatgacgt cctggagaag
     4621 agcggctacc gggccatgct ggccgatggc tcgcaggagg gcgaggatcg ctgggccaac
     4681 ctgctcgagc tgcgcgaggt cgtcacccgg tacggcgacc tggatcccga cgatgcggtc
     4741 gaccgcctgc tcgaggagac ggctctcgtc gccgaccagg actcctatga gaaggactcg
     4801 gacgccgtca cgctcatcac cctccacgct gcgaagggcc tcgagttcga cgccgtgttc
     4861 atcagcggtc tggaggaggg cgtcttcccg cactcgcgcg cgcttgacga tcagcgccag
     4921 atggaggagg agcgccgcct cgcatacgtg ggcctgaccc gcgcgcggca ccggctgtac
     4981 ctgacccatg ccgcgacgcg cgcgacgtgg ggccgcggcg gcttctccat cccgtcgcgc
     5041 ttcctgctcg agatcccgtc cgacctgatg catgggccca ggctcgtggc accggacgag
     5101 ggcttcaccg acgacgatca gcgcccggtc gatgagcgca acggctacga cctgagcacg
     5161 atcctcgggc cacgcggcgg ctcgcggctc gtcgggcggc gtggacagtg gggcggcgcg
     5221 ggagcgaccg gcaacggtgg tggcgcactg accccgggcc ggcgcatcct gccgcccggc
     5281 ggcggctaca cgccgcctgt cggcgcgcca cgcgagggcg agtcgtttcg cccgtcgcgc
     5341 gacctcgccg cgaaacgcgc ggcttactac ggggaggcgg acgcccctcc ggccatggcg
     5401 gccaggcagg tcgcgccaca gcgacccgtg gtccccgggg agcggcgcta ccgcgacggc
     5461 gatcgcgtgc gccacgccgc atttggcgaa ggcaccgtcg tgacatcgaa gctgacgcgc
     5521 gacgacgagg aggtcaccgt cgccttcccg ggagcgggca tcaagaagct gctcgcctcc
     5581 ttcgccaacc tggagctgct tgacggctga ccacttgacg ccagaccact tgcggcgcgg
     5641 gcattgaccg cagggcgaag atacgaggac ccgatcgggg gactacagag ggctgcgaca
     5701 tgcctgttcc gagcgcagcg ggcaaaccgt gggaggggat ggactggcgt gacgagggca
     5761 cgccggagtg gcgcgactca ctgtccgaag agcaggccga cgagtggcgc aactggcagt
     5821 acgactcgca gcaggaggcc gcaggcggcg gcccggctgg cggtggcgcc cgtggcgagc
     5881 ccgcggggga ggattacgag cctccaaccg actcgtccta tcaacccggc tcgggcggac
     5941 ctgatgccca gagctgggac tacgactcgc ttccggatgg ggcgcgggag ccagccgtcg
     6001 acagcgctcc gtctgagcct gagctgccgt ccgagccgcc cgaccccacc ggcaagccgt
     6061 gggaaggcat ggatcccctc ggcgatttct cgaccgagtt caggctttcg ctgtccgatg
     6121 ccgaggccga cgatttcgcg cagtggcgcg actggcacga gcagaaggcc gaagaagagg
     6181 agcgcgccca ggacgagcaa gagcagcgcg agcgcgacga gcgcctgcgg cgtgagcagg
     6241 aggaggagaa acgcaccggg caggttgacc agtaccgcct cgaccacggt gccccggcac
     6301 cgccgggcaa ggcggtcgct ttcatcgacg atcagggcaa tccggtcgat gccgtcgtca
     6361 aggaccagcc gatcaagatc caggtcacgc tgccgcgaac ggagggccag gaccctccgc
     6421 ggacgatcga cgtggtcgta tccggacctc ggctgacgtt catcgggcgc gagtacagcg
     6481 gcaccgactc ggtgactctc gagtggatcg gcaatgttga cggtccggcc atctaccgca
     6541 gcgggtcgat gaccgtcgaa aacggtggcg agggtgtcag cagcctcgat gtcggcggct
     6601 tcgagttcaa caccggcggc ggcatgggcg gcttcagcat cgatgacggt gacttcgttc
     6661 aggtcgagat cgaaggcgtg accaacggca tacaggtctt cacgtcagtg gcgaagctcg
     6721 agatagccct gacgctaacc gcgatcaata cgtcgcatca gtcctacacc aatgtgctca
     6781 tcaacctcga ttcgctgatt gagcaggtga aggcggcgcc ggagggcaag gaaaaggccg
     6841 atctcctgca gcagctcacc ttcacgcgcg acaaggccca gcaggtcgtg acgctggcca
     6901 acaaggcgat tcaccacttc aactatgtcg atgcgctcga ccaccagcac ctcgcggtgc
     6961 tgcgtctgta cgcgacgtgg atgggcctct ttcgtgagcc aactgaaggc cccgatggca
     7021 agaagaccac cgagttcgga ccgctgttcg tgcccgatcc gcgcgatgag aacgacttcg
     7081 tcaaggacga gtcgaacgcc gcgaaagagg aggtccggcg catcttctgg gcgggcctga
     7141 cgcaggccac catcggtggt taccgattgc tggcgcaggc gacgatggtc gcccagatca
     7201 tgaccatctt cgggacgaac gagatgggcc agcgcgcgag cggcatggag cgctggatgg
     7261 ccttcttcga tctgctcagc caggcggcgc tgatgggcgc catgatgaag tggaacacct
     7321 cgatggccac cgggacaggc ctgcccacag ggatcgtgcc gcgtaccacc gggcgcatcc
     7381 cgcgacagaa gccgctgacc gacacggcga cggcaaagtc ggcgcccagc ggcactccgg
     7441 tagcgcctga ggcggcgggc atgctccgcc acggagcgcg cgacggccag ttagtggcac
     7501 tcaagaacga cgccgtgatc caggtgcgct tcaccagccc agacgcgatg ccgctgcgcg
     7561 cgcagggctt cacgcccaag agcccactcg tcaagtcaaa gacgatcaag gagatcgacc
     7621 gccagatcgg cgctcccgat gccgcccaga acggcgccgc cgggttcttt gagcccaagc
     7681 tgcccgagcg cgggacgatg acggacgccc aatgggcgaa ggtgcaggag cgcttcgacc
     7741 tgcgccaggg ggagtacgac gggccgatcg gccagtacca gcgcaagctc gccgatgagg
     7801 gcaagctcca gctgcgcgac gggatggtgt gcgacaagga cggaacgccg ttcgcgggtg
     7861 actacgacct gttcgagatc actaaccgcg acggcacgcc ggtctcagcc gaaaagtacg
     7921 acgcagtcgt cgacgacttg atgaacgcac ccggcttcca ggcggcgcac ggcgcccaca
     7981 tgcgctggaa tcccaggccg accgacccgg cctaccacct ggacatgtcg ggcgacacgc
     8041 agctcgcgct cgatgccgtg gccaagtggc aggcggatcg cggcatcttc cgggacatcg
     8101 tggcccagca ccagccgggc ggcgaagggt tgatcacctt cgcgcccaac cagccaccgg
     8161 tcatcacgta cgccggtgag gcggtcgggc ccaagttcgc cgatcttggc atcacaccga
     8221 tcgcgccgag cgccggcacc ggcccaaccg tattcgtgac gccgcggcta tttgtggcca
     8281 ccacgcagca attgctggac ggccgcttcc ccgatggcgc cgagtgggaa tggacgcgag
     8341 cctgggactg gcagtcggaa gtggccgact ggcagcaaaa ggccgacgca ctggacaccg
     8401 gagcaatcac cgtgacggcc gacgatgcgc cgatagtgga cgtgacgagg agcgtcagcg
     8461 ccataggtgc cccgctgccc ttgacgatcc agccgaggtc cggcggcttc aactggaaac
     8521 cgatcgcgat cggtggcgtt ctcttgctgg cggtcgcggg agggggcttc gtcctgctca
     8581 accagccggg gccgggcccg ggcatcgccc tcggcagccc gaccaacgcc ccgttgagca
     8641 cccagccgac ccagccaccg acccagccgc cgaccacgcc tccgactgca ccgcccctga
     8701 cggctccgcc cctaaatccg agcctgaccg ccgtcaacac tgtgatcggc gagtggtcag
     8761 gtgggcgcgt ggagctcccg ccgctttgga ccgttgggag ggaccacctg gccgacgact
     8821 cagacgacct gtacctgccg aacgatggct ccgcggccac cgcgatccaa cagcacgtcg
     8881 acatgaccgg agtattcgcc ctcgtggcgg acttcacggc ggctgatgcg gtggggcttt
     8941 cgctcaaccc gtgcgcgggc acgtttgaga gcccgggcgg cttcacgttc aacacggcct
     9001 gcaaccagaa cgtgcctctc gcggcaggcc agtacctcgt cgtcgtgacc acgtgggcgc
     9061 aagccatgcc ggatcctttc ccggagaccg cccagtgctc aatctcggtg cagtcggacg
     9121 tggacaacga cccgacaacc ggtttccaat caaacctgcc gttcaactac ctcatcggcg
     9181 ctgacgtcta ttacgagaac ctcgtgttcg tgagcaacgc cggtgaatac cagaactaca
     9241 tcctggccac cgactactcg ctccccccgc gcagcgacac aggcgagatt cacggcaata
     9301 agccctttgc cggtcgtgtc atctggggca gtgacgccgg gacgccggcg gtcggggcga
     9361 cgcagacgtt cctcattcct ctcgatgagg tcggcgactc gttcgccgtc ggtggcttct
     9421 gcaccaccga ccgaggccgg atcgggcccg acacgctggc ggtggatgcg atcggtgctc
     9481 ctgaggacga cgacttcccg tactacctgg acgtcgccga cgtcttcttc ccgccacgcg
     9541 ttccgtaggc gcctgacccg cgaactgcta actcgccgac tttgactatg ctcgggccgt
     9601 gacccctcag aaggccgcgg cccgtgtcgc cgaactgcgt gagcagatcg agcgcgccct
     9661 caagcagtat cacgagctgg atgcgcccga gatcacggac gccgagtacg acgcgctata
     9721 ccgggagctg gtcgagctcg aagaggcgca tccggagctg cacacgcccg actcgcccac
     9781 gcagcgcgtt ggcggcgcgc ccagcggcgc cctggcggag gtccgccact ccacgccgat
     9841 gctctcactc aacaacgcgt tctcgttcga cgagctgcgg gcattcgacg ctcgcgcccg
     9901 ccggctgctc gatctggccg aggaggatgc agccaggacg ctgcgctacg agtgcgagct
     9961 caagatcgac ggcctggcga taagcctcag gtatgtccgc ggccggttcg ttcagggcgc
    10021 cacgcgcggt gacggcacca ccggcgaaga cgtgacggcc aatctccgaa cgatcgagtc
    10081 agtgccaaaa gtgctcacgg agccggtgag catcgaggtg cgcggcgaga tctacatgcc
    10141 caaggccgag ttcgcgcgca tcaacgccga gcgtgaggaa gccggcctgc cgctgtacgc
    10201 caatccgcgc aactcggggg ccggctcgct caggcagatc gacccgcaag tgacggccag
    10261 ccgccgactc gccgcatggt tctacgtcct ggtggaggcc ggcagcgcga cgacgcagac
    10321 ggcagcgctg gatcggctcg agaagctcgg cttcccggtc gagccgaatc gctcaacaac
    10381 agatctcgac atggacggcg tgacggcctt catcgagcac tggcaggagc cacgccacga
    10441 tctggcgtac gagaccgacg gggtcgtggt caaggtcaat cgcttcgacc tgcagcgaag
    10501 cctcggaatg gtctcgcacg cgccgcgctg ggcgatcgcg tacaagtacc cgcccgagca
    10561 ggtggagacc gtgatcgagg acatagtcgc ctacgtcggc cgcaccggga cgctcacgcc
    10621 cgtcgcgcgg atgacgccga ccaaggtcgc gggctcgacc gtcgccaacg ccaccttgca
    10681 caacctcgac gaagtgcggc gcaaggacat ccgtgtcggc gaccacgtcg tgctgcacaa
    10741 ggccggcgac gtcatcccgg aagtggtgcg ggtcatcctc gagaagcggc ccaagggcgc
    10801 gaaggaatgg cagatgcccg ccacttgccc ggtgtgcggc acggccatcg tgcgcgacga
    10861 gaacgcggtg cgccactact gccccaacct ggcatgtccc gcgcgcgttg gccaggagct
    10921 gggccatttc gtcggccgcg gctcgatgga tatcgagggc gcgggctgga aggtgctcgt
    10981 ccagctgatt gagcgcggcc ttgtcaagac gcgtggcgac ttcttccgcc tgagcgtcga
    11041 ggacatcgag tcgctggacc gctttgcccg caagagcgcg gagaacctgc atgcgtccat
    11101 ccagaaggcg cgcgtcaggc cgctctaccg gatcatccac ggcctcggca tcccccaggt
    11161 cggcgtcacc acggcgatcg agctgtccaa ctggctcgcg gcacgctggc cgccgcgcga
    11221 ggacgagccg atgggcggac aggacggctg gttcaagcgg atcgcgactg agctgcgcac
    11281 ggttccagcc acggatttcg agcagatccc gggcgtaggt cccacggttg ccgcggcgct
    11341 ggagcgctgg ctgaccgacc cgctgaccgc ggacgtgctc gacgatctcg tcgacgccgg
    11401 cgtcgagcct gagcgaccgc gcgcacaggc cgccgctaac acggaaggcc cactcagcgg
    11461 caagacggtg gtcgtgacgg gcacgctgaa gggcttcgat cgcgaacagg ccgagcaggc
    11521 gatccgcgcc gccggcggca aaccgggcgg gtcggtgagc aagaagacca actatctggt
    11581 cgcaggcgat tcggccggat cgaagctggc gaaggcgcag gagctgggcg tggcggtcat
    11641 cgacgaggag ggcttccgga aggtcctggc gggcgaggaa ccgtagctga tggatctcca
    11701 tcatctggcc gacgtgctgg ccgagcaatc ggccggcggt cgcgcctacc tggagttcct
    11761 gcgctccgat tcactgtctg ttggcctgta cgtcctccgt gtcggtgacg tcgatcgcca
    11821 gcagccgcac cgcgaggacg agatctacgt cgtgatgtca ggtcggtcgc ggttcacggc
    11881 gggcgaagac acgcgccagg tcacggctgg cgacactatt ttcgtcgcgg caggggtacc
    11941 gcatcgcttc cacgac
//
LOCUS       DATBRX010000147         6323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2842755, whole genome shotgun sequence.
ACCESSION   DATBRX010000147 DATBRX010000000
VERSION     DATBRX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2842755"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1694
                     /locus_tag="VIF08_05650"
     CDS             222..1694
                     /locus_tag="VIF08_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEY8135506.1"
                     /translation="MSDAYVDPPTQPNPADAPPVPIDVLDIARNGLGARPGPAKRVLI
                     IGGGMAGLVAAYELRRQGHDPLVLEAQGRVGGRIYTLRAPFGPGLYAEAGAMRIPRSH
                     ELTLEYCRKFDLPMRPFVMGNPRGLVHIGGQRMTAAEANEHPENLPFNLTPSEHGRSA
                     DSLWNDAIADLRALVEKDGEAAWPEIVRQYDEYSLYEFLRYKGFSEGAIEFYAVMNFV
                     ESDLHNSFVEVLREELSGAYVDMQTIAGGMDALPNALYNAMPEVVRFGAEVRAMQQDD
                     RGVTVHCRVGPDQVTFSGDYAICTVPFSVLRPIEHRFSREKERAVRQLNYHASTKILM
                     QVRERIWEAEDGIVGGASVTDLPVRRLNYPPSDDSTTRGILLASYTWGQDALQWGAMD
                     EETRVEEALDDVEKIHPRIREVFEVGASHAWYSDRWARGAFALFAPEQQTSLQQAIVQ
                     PEGRIYFAGEHCSLYHAWIQGSIESALRVSREIHELPPAA"
     gene            1691..2047
                     /locus_tag="VIF08_05655"
     CDS             1691..2047
                     /locus_tag="VIF08_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8135507.1"
                     /translation="MTSRLEAFTRWLTAFGDAWETGDASRLSPLFTVAATFAPDPFAD
                     VVRGRKALLAFCDELFAAWPDASFSAQVLGVGDTYGVAHWRVSSEERAMDGVWVVALD
                     ARGRCESLREWSSESR"
     gene            2355..2807
                     /locus_tag="VIF08_05660"
     CDS             2355..2807
                     /locus_tag="VIF08_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8135508.1"
                     /translation="MNHVVLTGRLTRDPESRKLASGKTVTEFSVATNDYRGGTEKAEY
                     HPVVTWDRLAEICGQYLGKGQLVSIIGRLQTRQWEDADKKRHWKTEIVANSVEMLSGK
                     KKKDYAAESAAEALAAQAAAMGVEADTLAFSASADHESTEAEEEALAA"
     gene            2904..3911
                     /locus_tag="VIF08_05665"
     CDS             2904..3911
                     /locus_tag="VIF08_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135509.1"
                     /translation="MRPIGLLLAFVVLLAGAYVMAQPVAAPSASPTVPALAAATATAT
                     PAATATAMPTGAVLASPSSGLVPTPTNTALPTPTTFVLPSTSPGLAVPKAVIIVAPTG
                     ELTEGNLTLGEEMALRAEAAGMDVHRVFYPHATWEVVLAEIQGASLVAYYGHGYGWPS
                     NTPELWEVQQDGMGLNAYDGSGEYEVHYYGANKIRDSIRLAPHAAVFLMRGCYTAGNG
                     LNEDPIPPLDTARQHVDNYASGWLAVGAGAVFAFATGSRTSYPEQLMAGDAATVDDLF
                     MNAGDAIGTNTLYFDSQRTPGARNHLDPHPTKGYLRALSGDLGMTAAIWRSGAGAIVT
                     V"
     gene            3912..5039
                     /locus_tag="VIF08_05670"
     CDS             3912..5039
                     /locus_tag="VIF08_05670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8135510.1"
                     /translation="MDRADVVVIGGGIVGTSAAAFLAQAGKRVVLVEREGLASAASGA
                     NSGVVQHPFDPVLAALYHSTIELYRELSLTSTSFKLADQPGGMLFVTEREDAARAEAA
                     SVGAAFPDLTVDVLADAALRNLEHALAPGLWACRVDIGYPIWPGASTYAYASLAEERG
                     VTIRMGRAAALSREGDAVGGVVVDGRHISAAKVLVAAGPWSPEVIDPTGSWRPIRPLW
                     GSVVEVELANAPRHVLEQAGIQASIGAAAGEHGPRDHDFSLVSVPGVSVVGSTFLDEE
                     PDPLEWREPILRFAARFVPGILDAPIRGVRACPRPLSADGLPLIGRVPGSANLYICAG
                     HGPWGISTGPGSARLVTDLMVGGAAAIPPELAPERFGPLPD"
     gene            complement(5036..5731)
                     /locus_tag="VIF08_05675"
     CDS             complement(5036..5731)
                     /locus_tag="VIF08_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY8135511.1"
                     /translation="MPQHVQGGDAVDYAHAVLRQPAPSGIRTRLILVLSMSLVLAGLL
                     PSPVSAGWLSSTWPAYEATPNSEAARVLEIAHEQLGDRFVMGAIGPDAFDCSGLVWYA
                     FKNAGLADRMGGKRRGARSYLNWFKANLPKQVSHNLADARPGDILIWGGGHHSGLFIG
                     GKWAISALNPRYDIRIHRADRVGLPFTAVLMVNISRTDEGSTPTPSPTPTPTATPATS
                     ASPSAAPSTAPAH"
     gene            complement(5912..>6323)
                     /locus_tag="VIF08_05680"
     CDS             complement(5912..>6323)
                     /locus_tag="VIF08_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135512.1"
                     /translation="YDTFVDHPWLPGQYTYAIQTVDANGVRSFRSEEVAVDVYFPGTP
                     TNVNDTTPPTVPTGLTAQSLGDKQVALSWQPSTDTSDVGYLVKRGSKIIARVFGTNYV
                     DRPSKAGAYSYSVVAFDAYGNTVASAKVDGVAAL"
BASE COUNT          999 a         2079 c         2236 g         1009 t
ORIGIN      
        1 tgcggtcggc gtcgcgctgg cggggttgct gggcagtgaa ctgggtgtgc ccatcggggc
       61 aacggtcggg atcgggctgg cagtcggtac caccgggctc gtcgtgcagc ccgcgagaat
      121 gaccacgatg acggtgccca ggcgtcgcat ggtggcgtca gcgtaacgcg ggccaagtac
      181 gatctgcgct ggatagccgg aacttaacgg agactgaccc gatgagcgac gcgtacgtcg
      241 atccgcccac ccagcccaac ccggcggatg cgccgccggt gccgatcgac gtgctcgaca
      301 tcgcccgcaa cggactcggc gcgcggcctg gtccggccaa gcgagtgctg atcatcggcg
      361 gcggaatggc cggcctggtc gccgcgtacg agctgcgccg ccagggccac gatccgctgg
      421 tgctcgaggc ccagggtcgt gtcggcgggc ggatctacac gctgcgcgcg ccattcggtc
      481 cggggttgta cgccgaggcc ggcgccatgc gcatcccgcg ctcgcacgag ctcacgctcg
      541 agtactgccg caagttcgat ctgcccatgc gccccttcgt catgggcaac ccacgcggcc
      601 tggtgcacat cggtggccag cgcatgaccg cggcggaggc gaacgagcat ccggaaaacc
      661 tgcccttcaa cctgacgccc agcgagcacg gccggtcggc cgactcgctg tggaatgacg
      721 cgatcgccga cctgcgcgcg ctggtcgaga aggacggcga ggccgcgtgg ccggagatcg
      781 tgcgccagta cgacgagtac tcgctctacg agttcctgcg ctacaagggc ttcagcgagg
      841 gcgcgatcga gttctacgcc gtcatgaact tcgttgagag cgacctgcac aactcgttcg
      901 tcgaggtgct gcgcgaggag ctgagcggcg catacgtcga catgcagacg atcgccggtg
      961 gcatggacgc gctgcccaac gcgctctata acgcgatgcc cgaagtggtt cgcttcggcg
     1021 ccgaggtgcg ggcgatgcag caggacgatc gcggcgtcac cgtccactgc cgggtcgggc
     1081 ccgaccaggt caccttcagc ggcgactacg ccatctgcac tgtgcctttc agcgtcctgc
     1141 gcccgatcga gcaccgcttc tcacgcgaga aggagcgggc cgtccgccag ctcaactacc
     1201 acgcgtcaac caagatcctg atgcaagtcc gcgagcgcat ctgggaagcg gaggacggga
     1261 tcgtcggcgg cgcgagcgtg accgatctgc cggtgcgccg cctcaactat ccgccgagcg
     1321 acgactcgac gacgcgcggc atcctgctgg cctcctatac gtggggccag gacgcgctcc
     1381 agtggggcgc catggacgag gagacgcggg tcgaagaggc gctcgacgat gtcgagaaga
     1441 tccacccgcg catccgcgag gtattcgagg tgggcgcgtc gcacgcctgg tacagcgatc
     1501 gctgggcgcg cggcgccttc gcgcttttcg cgcccgagca gcagacctcg ttgcaacagg
     1561 cgatcgtgca acccgagggc cggatctact tcgcgggcga gcactgctcg ctctaccacg
     1621 cctggatcca gggctcgatc gagtctgcgc tgcgggtgtc gcgcgagatc cacgagctgc
     1681 cgccggccgc gtgacctcgc ggctggaggc cttcacccgc tggctgacgg cgttcggcga
     1741 cgcatgggag accggtgacg cgagccggct gagtccgttg ttcacggttg ccgcaacctt
     1801 cgcgcccgat ccatttgccg acgtggtccg tggccgcaag gctctgctgg cgttctgcga
     1861 cgagctgttc gcggcgtggc cggacgcgtc gttttcggcg caggtcctgg gcgttggtga
     1921 cacctacggc gtggcccact ggcgcgtttc ttcggaggag cgagccatgg acggcgtctg
     1981 ggtggtcgcg ctcgacgcgc gcggccgctg cgaatcgctg cgcgaatggt ccagcgagtc
     2041 gcggtaagac aatcatgctg ggttcctgac aaatgccgcc cgggacgtgg gcgagcgggg
     2101 atcgggccac gtatagggtc gattcttttg acacacgcgg cctgcgcagg gcatttgtcg
     2161 gaggatcggg aaatttcgac ggcacaggct gctcaccgac agcaaatggc aggaagcggc
     2221 cgcgaaatgg tggataagcc gataagcgaa agagatgccg ccgcggctat ggtccgtcgg
     2281 gtctagttcc cgccgcccgc cgagaacgca ggccgagccg ggttgtgccg gccggtcagg
     2341 aggtatggag cggcatgaac catgtcgtac tcacagggcg gctgacgcgc gacccggagt
     2401 cgcgcaagct ggccagcggc aagacggtca cggagttctc cgtggccacg aacgactacc
     2461 gcggtggcac ggagaaggcc gagtaccacc ccgtcgtcac ctgggatcgg ttggccgaga
     2521 tctgtggcca atacctgggc aagggccagc tggtgagcat catcggccgg ctgcagacgc
     2581 gccagtggga ggacgccgac aagaagcgcc actggaagac cgagatcgtc gccaactcgg
     2641 tcgagatgct gtcaggcaag aagaagaagg actacgccgc cgagtccgca gccgaggcgc
     2701 tcgccgccca ggctgcggcg atgggggtgg aggccgacac actggccttc agcgcctcag
     2761 ccgatcacga atccaccgag gcggaggagg aagctctggc cgcctagagc cccagcagac
     2821 ggcgcggcgg gcgcttctct cctcgcgtcc gccgcgtcgc tctttcatgg cgattgggcc
     2881 gtgtcaggca gaatcaacag gtcatgcgcc caattggcct gctgctggcc tttgtcgtgc
     2941 tgctggcggg cgcttatgta atggctcagc ctgtcgcggc accatctgcc tcgcccaccg
     3001 ttcccgcgtt ggcggccgcg acggcaaccg cgacgccagc tgccactgcg actgcgatgc
     3061 cgaccggtgc ggtgctggcc tcgccgagca gcgggctcgt gccgacgccg acgaacaccg
     3121 ccttgccgac gccgaccacc tttgtgctgc ccagcacctc accaggtctg gccgtgccca
     3181 aggcggtcat catcgtcgcg ccgaccggcg agctgacgga aggcaacctg accctcggcg
     3241 aggagatggc cctgcgcgcc gaggcggccg gcatggatgt ccatcgggtg ttctatccgc
     3301 atgcgacctg ggaggtagtg ctggctgaga tccagggtgc gagccttgtc gcgtactacg
     3361 gccacggcta cggctggccc agcaacaccc ccgagctatg ggaggtccag caggacggca
     3421 tgggcctgaa cgcgtacgac ggcagtggtg aatacgaggt ccactactac ggcgcgaaca
     3481 agatcaggga ctcgatccgc ctcgcgccgc acgctgccgt cttcctgatg cgcggctgct
     3541 acacggcggg taacggcctc aacgaggatc ccattccgcc gctggacact gcgcgccagc
     3601 acgtcgacaa ctacgcaagc ggctggctgg cggtcggcgc gggcgccgta ttcgccttcg
     3661 ccacgggctc gcgcaccagc tacccggaac aattgatggc cggcgacgcg gcgacagtcg
     3721 atgatctgtt catgaacgcc ggcgatgcaa tcggcaccaa cacgctgtat ttcgactcac
     3781 agcgcacgcc cggcgcgcgc aaccacctcg acccgcatcc aacgaagggc tacctgcgcg
     3841 cgctcagcgg tgatctgggg atgacggcgg ccatatggcg ctccggcgcc ggcgcgatcg
     3901 tcaccgtcta ggtggaccgc gccgacgtcg tcgtcatcgg gggcgggata gtcggcactt
     3961 ctgcggccgc cttcctggca caggccggca agcgggtggt cctcgtcgag cgcgagggcc
     4021 tcgcctcagc ggcgtcgggc gcgaactcgg gcgtggtcca gcatccgttc gacccggtcc
     4081 tggccgctct ctaccactca acgatcgagc tgtaccgcga gctgtcgctc acatcgacga
     4141 gcttcaagct ggccgatcaa ccgggcggca tgcttttcgt cacggagcgc gaggacgcgg
     4201 cgcgcgcgga ggcagcaagc gtcggcgcgg cgtttccgga cttgacggtg gacgtgctcg
     4261 ccgacgccgc gctgcgaaac ttggagcacg cgctcgcgcc cggcctgtgg gcgtgccgcg
     4321 tggatatcgg ctaccccatc tggccgggcg cgtccacgta tgcgtatgcg tcgctggcgg
     4381 aggagcgcgg cgtgaccata cgcatgggcc gcgccgcggc gctcagcagg gaaggggacg
     4441 cggtcggcgg cgtcgtcgtg gatgggcgcc acatcagcgc tgccaaggtg cttgtcgcgg
     4501 ccggtccgtg gtcgcccgag gtgatcgacc caacaggatc ctggcggccg atccggccgc
     4561 tgtggggatc agtcgtcgag gtggagctgg cgaatgcccc gcgccacgtt cttgagcagg
     4621 cgggcatcca ggcgtcgatc ggcgccgccg cgggtgagca cgggccgcgg gaccacgact
     4681 tcagcctcgt atcggtcccc ggcgtcagcg tcgtcgggtc gacgttcctg gacgaggagc
     4741 cggatccgct cgagtggcgc gagcccatcc tgcgcttcgc cgctcgtttc gtgccgggca
     4801 tcctcgatgc gcccatccgc ggcgtgcgcg cctgcccgcg gccgctgagc gcggacgggc
     4861 tgccgcttat cgggcgcgtc ccgggcagcg ccaacctcta catctgcgcc ggccacggac
     4921 cgtggggcat ctcgaccggg ccgggctcag ccaggctcgt caccgacctg atggtgggtg
     4981 gcgccgccgc gatcccgcct gagctcgcgc ccgagaggtt cgggccgctg ccggactagt
     5041 gcgccggcgc ggtgctcggg gcggcgcttg gtgaagcgct ggtcgcgggc gtcgcggtcg
     5101 gagtaggtgt tggcgaaggc gttggtgtgg agccttcgtc ggtgcgcgag atgttcacca
     5161 tcagcaccgc ggtaaaaggc aggccgacgc ggtcggcgcg atggatgcgg atgtcatagc
     5221 gcgggttgag cgcgctgatc gcccacttgc cgccgatgaa caggcccgaa tggtgtccgc
     5281 caccccagat gaggatgtcg cccggacgcg cgtcagcgag gttgtgtgag acctgcttgg
     5341 gcagattggc cttgaaccag ttgagatagc ttcgcgcgcc gcggcgcttg ccgcccatgc
     5401 gatcggccag gccggcgttc ttgaaggcgt accagacgag gcccgagcaa tcaaaggcgt
     5461 cgggaccgat ggcacccatc acgaagcggt cgccgagctg ttcatgggcg atctcgagca
     5521 cccgcgcagc ttccgagttg ggcgtggcct cgtaggccgg ccatgtgctg ctgagccagc
     5581 cggcacttac cggactcggc agcaggcccg cgaggaccag tgacatggac agcacaagaa
     5641 tgagtcgtgt cctgatcccg ctcggcgcgg gctggcgcaa cacggcgtgc gcgtaatcca
     5701 cggcatcccc tccctgcacg tgctggggca cggacagatg gctcccgaga cgtcaccgaa
     5761 gtgctggggc actcgattga cttgtgtctg taacgacttt acccggcggc gacccggttt
     5821 ggcaacctta caagacacga gaccggcccc gtcagggccg gtctctgctt tcagtctgag
     5881 tggcgcggcc gcgatcgccg cgtcggtggg cctacagggc ggccactccg tccaccttcg
     5941 ccgacgcgac ggtgttgccg tacgcgtcga aggccacgac gctgtaggag taggcgccgg
     6001 cctttgaggg ccgatcgacg tagttggtcc cgaagacgcg cgcgatgatc ttggagccgc
     6061 gcttgaccag gtagcccacg tcgctcgtat ccgtgctggg ctgccagctc aacgcgacct
     6121 gcttgtcacc gagcgattgg gcggtcaaac cggtgggcac cgtcggtggg gtggtgtcat
     6181 ttacgttggt cggcgtaccc gggaagtaca cgtccaccgc gacctcctcg gagcggaagc
     6241 tgcggacgcc gttggcgtcg acggtttgga tcgcgtaggt gtactggccg ggcagccagg
     6301 ggtgatcgac aaaggtgtcg tac
//
LOCUS       DATBRX010000148         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_721834, whole genome shotgun sequence.
ACCESSION   DATBRX010000148 DATBRX010000000
VERSION     DATBRX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721834"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VIF08_05685"
     CDS             <1..1585
                     /locus_tag="VIF08_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135513.1"
                     /translation="VDGWLTQQGGEVLCQPPPQNVAAGLPSLWCGNAAWLFDEPVDAN
                     DPDNPDFDSSGGLPVQNNAYDEIIGATGRGPVPSLRATYAVAPRLFTGCGAEPTCWHW
                     AIVALVDGVSATSPSPSPTSSDVSRTIGCDPGPSFVDEAGVVASCTATKSESQDELPL
                     TVGNPGGDQSLLEITWAGGYSDLDRLVLRRGGAGYELVALHASPINAIIDAAVFETKL
                     RLQLSAPVSADSVSVVFEWPTSEPSPPAPTLPPVATPSLEPVTIECTDTVGLGRTMIV
                     LDDTNLVGGCRMIRPALIQSATVTNPEGDLSRLRVEWPSSTGVEYVSVRLSRQSEGYV
                     VEVASHDRCQPAGYVCAGLYFVGVELDLRTDVDASGVAINFFAHDGREMDIAASKDGV
                     EFTLEIPADKTEYTQSEPIDVVAALTSDADVTVTCLSGPIISLEQLDGPITFEPGPFI
                     LLCPGSRDLRSGESIAHGFSPTAWGSAEPNPLDPYVRDGKLYLPAGTYRFHAGSSFTV
                     GEIGGEQFRLEASIVVTVR"
     gene            1640..2821
                     /locus_tag="VIF08_05690"
     CDS             1640..2821
                     /locus_tag="VIF08_05690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8135514.1"
                     /translation="MKLAQRMVGIGTETAFEAAARGRALEAQGRHIVHLEIGEPDFDT
                     PVNIREAAKKALDEGWTHYGPPLGQPKLREAIAKESLRSRGFEADPANVVVTPGAKPI
                     MYYAILALIEPGDEVIYPDPGFPIYESMTRFTGGTPVPYALREENDFRFDVDELRALV
                     TPKTKLIIFNSPHNPTGGVLTREDITAIADIAREHDITVMADEIYGRGVFEGEHFSIA
                     TLSGMAERTIILDGFSKAWAMTGWRLGFAILPPALVDPFSKLIINSVSCTSSFEQIAA
                     IEALNGPQDEVARMVEEFRVRRGIIVDGLNAIPGITCRMPHGAFYVFPNVSGTGMNGT
                     QMADKLLYEGGVCTLSGTAFGKVGADHIRISYANSRENLQLALERIAETVAKIPAGAS
                     A"
     gene            2880..3701
                     /locus_tag="VIF08_05695"
     CDS             2880..3701
                     /locus_tag="VIF08_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8135515.1"
                     /translation="MPLTALAIALGAAVIHALWNLVIARSPDNQATTAVAIFAGTLVA
                     LPLALLRWNVQPEAWPWIIASSVLELVYFYLLTAAYRRAEMSLVYPIARGMAPVIVLI
                     VSIAFGVAFHPWQIAGVALVGGGVMLVRGIRGGARWSDVVLALAVATSIAGYTLVDKQ
                     GVRYADPATYLFLILIAPAVAGVFFVAARGGWARVRKAVNWQAVVAGIGTTAAYTLVL
                     VALSLAPAASVAAVREVSVVLAVFLGAMFLKEGVGASRWIGALVVAAGVVLVVLG"
     gene            complement(3705..>4782)
                     /locus_tag="VIF08_05700"
     CDS             complement(3705..>4782)
                     /locus_tag="VIF08_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135516.1"
                     /translation="AEYGRPLFIGASADLAESTNLAGFGKDFGDLPGWGWYERDSNPR
                     GTVLPTQITEFTNAGLAVGMATVNMAANPFDDFNGFWGTCSTYGSFSYLKYGPMRLFS
                     QLAQDCELKLGKVLWIAGHAGPETAEDSRTHFGIFETAVTQLFPEGHVIDLHPWEYNE
                     VPVLLAAALATEVPIVALHLTRPAVEIPDRAAMGMPSHFEAARGAYVMRPFRDGVAHG
                     GTVFVQGTTTTANVVKILPRLDELGLNVKIVICASPQLFAMQDEAYRESVASLGDRWD
                     GMAVTNRAWQTMRHWIGGPIAHDYSLSSDFDDRWRTGGTVEEVIDEAHLGPDHILAAI
                     QRFVTEREVRLARVRELAEAAAAN"
BASE COUNT          781 a         1581 c         1565 g          855 t
ORIGIN      
        1 ggtggatggt tggctgaccc agcagggtgg tgaagtgttg tgccagccgc cgccgcagaa
       61 cgtggccgct gggcttccgt ccctatggtg tggcaacgcc gcctggctgt tcgatgaacc
      121 ggtcgatgcc aacgatcccg acaaccccga tttcgacagc tccggtggct tgccggtcca
      181 gaacaacgcc tacgacgaga taatcggtgc caccgggcgt ggccctgtcc catcgttacg
      241 cgcgacctac gccgtagcgc ccaggttgtt cactggctgt ggcgctgagc cgacatgctg
      301 gcactgggca atcgttgcgc tcgtcgacgg agtgtcggct accagtcctt ctccttcgcc
      361 gacttcatcg gacgtttcgc gaacgatcgg ctgcgacccc gggccgagtt tcgtggacga
      421 agccggagtc gtcgcatcct gcacggccac caaatctgag tcacaagacg aactgccgct
      481 tactgtcggg aacccgggtg gcgaccaatc gttgctcgag atcacatggg ctggcggcta
      541 cagtgacttg gaccggctgg tgctcaggcg ggggggtgcc ggatatgagc ttgtcgctct
      601 ccacgcgagt ccgattaacg cgatcatcga cgcagccgtc ttcgagacca agctccgcct
      661 gcagctgtcc gcgccagtgt cggccgattc cgtatccgtc gtatttgaat ggccgacttc
      721 agagccgagt ccaccagcgc ccacgctacc gcccgtggcc acaccatcgc tcgagccggt
      781 aacgatcgag tgcaccgata ccgtggggtt aggccgaacc atgattgtgc tcgacgacac
      841 gaatcttgtt ggcggatgcc gaatgatcag gccggctctt atccagtcag cgacggtgac
      901 taacccggag ggtgacttgt cgcgattgcg cgtcgaatgg ccctcatcga ctggggtgga
      961 atacgtgtcg gttcggctat cccgccaatc tgagggttac gtggtcgagg tcgcgtcgca
     1021 tgatcgatgt cagccggcgg ggtatgtatg cgcggggctc tatttcgtgg gtgtcgaact
     1081 cgatctgcgg acggacgtcg acgcttctgg tgtcgcgatc aatttctttg cacacgacgg
     1141 ccgtgagatg gatatcgccg caagcaaaga cggcgttgaa ttcaccctgg agatcccggc
     1201 ggacaagacc gagtacacgc agagtgagcc catcgacgtt gttgcggcgc tgacttcgga
     1261 tgccgacgtg accgtgacgt gtctgtctgg gcccatcatc tcgctggagc aactcgatgg
     1321 cccgatcacc ttcgaacccg gtccgttcat cctcctgtgc ccgggatcga gagacttgcg
     1381 aagtggggag tcgatagcac acggattctc accgacggca tggggatccg cagaaccgaa
     1441 cccgctcgat ccgtatgttc gcgacggcaa gctttacctg ccggctggca cctatcgctt
     1501 tcatgcgggc tcaagcttca cggtcggcga aatcggcggg gagcagtttc ggctcgaggc
     1561 atcgatcgtg gtcaccgtgc ggtgatccgg tccgatcggc acttctgggg gcgaatcggc
     1621 ctttgctagg atgccggtca tgaagcttgc acagcgcatg gtggggatcg gtactgagac
     1681 cgccttcgag gctgccgcgc ggggtcgcgc gctggaggcc cagggccggc acatcgtcca
     1741 cctggagatc ggtgagcccg acttcgacac cccggtgaac atccgcgagg cggcgaagaa
     1801 agcactcgac gagggctgga cgcactacgg tccgccgctc ggccagccca agctgcgcga
     1861 ggcgatcgcc aaggagtcgc tccggtcgcg cggcttcgag gccgacccgg ccaacgtggt
     1921 cgtcacgccc ggcgctaagc cgatcatgta ctacgcgatc ctggccctga tcgagccagg
     1981 cgacgaggtc atctaccccg accccggctt cccgatctac gagtccatga cgcgcttcac
     2041 cggcggcacg cccgtgccct acgcgctgcg cgaggagaac gacttccgct tcgacgtcga
     2101 cgagctgcgt gcgctcgtga cgcccaagac caagctgatc atcttcaact cgccccacaa
     2161 cccgacgggc ggcgtcctga cccgcgaaga catcaccgca attgccgata tcgcgcgcga
     2221 gcacgacatc accgtgatgg ctgacgagat ctacggccgc ggcgtcttcg agggcgagca
     2281 cttctcgatc gcgacgctgt ccggcatggc cgagcggacg atcatcctcg acggcttcag
     2341 taaggcgtgg gcgatgaccg gctggcgcct gggcttcgcg atcctgccgc cggccctggt
     2401 cgatccgttc agcaagctga tcatcaacag cgtttcgtgc accagcagct tcgagcagat
     2461 cgcggcgatc gaggcgctca acgggccgca ggacgaagtc gcgcgcatgg tcgaagagtt
     2521 ccgcgtgcgg cgcgggatca tcgtggacgg cctcaacgcg atccccggca tcacctgtcg
     2581 catgccccat ggcgcgttct acgtcttccc caatgtcagc ggcacgggca tgaacggcac
     2641 ccagatggcc gacaagctgc tctacgaagg cggcgtatgc accctctccg gcactgcgtt
     2701 cggcaaggtt ggcgcggacc acatccgcat ctcctacgcc aactcgcgcg agaacctgca
     2761 gctggccctc gagcgcatcg ccgaaacggt agccaagatc cccgcgggcg catccgctta
     2821 gctcaccgac tgacccggct ggcacgcatt tgacgccagc cacccagggc cactaacgga
     2881 tgccgctcac cgcgctggcc atcgcacttg gcgcggccgt gatccacgcg ctgtggaacc
     2941 tcgtcatcgc gcgcagcccc gacaaccagg ccaccaccgc agtcgcaatc ttcgccggga
     3001 cgctcgtcgc gctgccgctc gcgctgctgc gctggaatgt ccagcctgag gcctggccgt
     3061 ggatcatcgc ctcgtcggtc ctggagctcg tctacttcta tctgctcact gcggcctacc
     3121 gccgcgcgga gatgagtctc gtctatccaa tcgcgcgcgg catggcgccg gtcatcgtgc
     3181 tgatcgtctc gatcgcgttc ggcgtcgcgt ttcacccgtg gcagatcgcg ggagtggcgc
     3241 tcgttggcgg cggggtgatg ctcgtgcgcg gcatcagggg cggcgcgcgc tggtcggacg
     3301 tcgtgctcgc gcttgccgtc gcgacgtcga tcgccggtta cacgcttgtc gacaagcagg
     3361 gcgtgcgcta cgccgacccg gcgacgtacc tgttcctgat cctcatcgcg cccgcagtcg
     3421 ccggcgtgtt cttcgtcgcg gcgcgcggcg gctgggcgcg cgtcaggaag gcagtcaact
     3481 ggcaggccgt cgtcgccggc atcggcacga cggccgccta cacgcttgtc ctggtggcgc
     3541 tgtcgctcgc gcccgcggcc agcgtggccg ccgtgcgcga agtcagcgtc gtcctggcag
     3601 tcttcctcgg cgcgatgttc ctgaaggaag gggtgggcgc gagccggtgg atcggtgccc
     3661 tggtcgtcgc ggccggagtg gtgctggtcg tattgggctg atcactagtt ggcggcggcg
     3721 gcctcagcca gctcccggac gcgcgccagg cgcacctcgc gctcagtgac gaagcgctgg
     3781 atcgcggcca ggatgtgatc cgggccgagg tgcgcttcgt cgatgacttc ctcgaccgtg
     3841 ccgccggtgc gccagcgatc atcgaaatca gacgagagtg agtaatcgtg ggcgatcggg
     3901 ccaccgatcc agtggcgcat cgtctgccac gcgcggttgg tcacggccat gccgtcccag
     3961 cgatcgccca gcgacgcgac ggactcgcgg tacgcctcat cctgcatggc gaacagctgc
     4021 ggtgacgcgc agatgacgat cttcacgttg aggcccagct catcgagccg gggcaggatc
     4081 ttgaccacgt tcgcggtcgt ggtcgtgccc tggacgaaca ccgtcccgcc atgggcgacc
     4141 ccgtcgcgga acggcctcat gacgtaggcc ccgcgcgccg cctcgaaatg cgatggcatg
     4201 cccatcgcag cgcgatccgg gatctccact gcgggccgcg tcaggtgcag cgcgacgatc
     4261 ggcacctcgg tcgcgagcgc ggccgccagc aggaccggca cctcgttgta ctcccacgga
     4321 tggagatcga tcacgtggcc ttcggggaag agctgcgtga ccgcggtctc gaagatgccg
     4381 aagtgcgtgc gcgagtcctc cgcggtctct ggccccgcgt gaccggcgat ccacagcacc
     4441 ttgcccagct tcagctcgca gtcctgcgca agctggctga acaggcgcat gggcccgtac
     4501 ttgagatacg agaacgaccc gtacgtcgag cacgtgcccc agaagccgtt gaaatcgtcg
     4561 aacgggttgg ccgccatgtt gaccgtggcc atgccgacgg cgaggccggc attcgtgaac
     4621 tcggtgatct gggtcggcag gaccgtgccg cgcgggttgg aatcgcgttc gtaccagccc
     4681 catccaggga ggtcgccgaa gtccttgccg aatccggcca ggttggtcga ttcggcgagg
     4741 tcggctgagg caccgatgaa cagcggtcgg ccgtactccg cc
//
LOCUS       DATBRX010000149        15449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1392908, whole genome shotgun sequence.
ACCESSION   DATBRX010000149 DATBRX010000000
VERSION     DATBRX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15449
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392908"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VIF08_05705"
     CDS             complement(<1..1167)
                     /locus_tag="VIF08_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY8135517.1"
                     /translation="MRVELLATPDCPHLEQAEEIAREALANDGRLGHSPAIERVYVSD
                     IDTAAGLGFHGSPTLRIDGRDVVPVPAGEGFHLGCRLYRQPDGSLDGVIPATVIAAEV
                     ERREEEAAAHEAAKFHPRALPAKLSRAFFLWASHRRFLGRLATAIPLTRRMVRRFVPG
                     ERLDDALATLEGLQAAGMRWTVDVLGESISSEAMATAAADRYIETLDALAERGLEANV
                     SVKLTQMGLAIDRDFCLANVRRIVDRARSGGAFVRIDMEDHTKTTKTLEIVRELHADY
                     QGVGAVIQSYLRRSADDVAALNAEGIRVRLCKGAYDEPASVAFRTREEVDRSYIELME
                     RLLTDGNYPALATHDDRLIDHALAFVMEHAIDPARYEFQMLYGVRRDLHERLVAL"
     gene            complement(1300..2142)
                     /gene="proC"
                     /locus_tag="VIF08_05710"
     CDS             complement(1300..2142)
                     /gene="proC"
                     /locus_tag="VIF08_05710"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY8135518.1"
                     /translation="MSLARSTIATVGSGVMAEAMIAGLLRGKQVAPNRIIATHPRADR
                     RRELISSYGISTVEDNASAVDKADVVVLAIKPQMLARVGREIAPALKRGKLVISIIAG
                     ATTTALENALGHDQIVRSMPNTPAQLGRGMTVWYATPRVTKTQRDQARVLLGSLGVQL
                     EVDDEKLVAMATAVSGTGPTYVFLVMEALIDAAVHLGFPRHVAHDLVIETLEGSTLFA
                     KSSGMHPAQLRNMVTSPGGTSAAALHELESGRLRTVLSEAVWAAFRRTEELGRTLEID
                     TSKG"
     gene            complement(2189..2404)
                     /locus_tag="VIF08_05715"
     CDS             complement(2189..2404)
                     /locus_tag="VIF08_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135519.1"
                     /translation="MSDSVREDIRTTSDEIEADAQLLAHVERRKQDPKANDEELQRLA
                     AQAEALGRRIGERTRIEKKLVDQAAKT"
     gene            complement(2427..2636)
                     /locus_tag="VIF08_05720"
     CDS             complement(2427..2636)
                     /locus_tag="VIF08_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135520.1"
                     /translation="MPPTLFINPPRDAAFVEMVTVESASATSPEELRSRLARHYPNVI
                     VRQRSLEGERAQVWYVYRDGHWVAD"
     gene            complement(2719..3609)
                     /locus_tag="VIF08_05725"
     CDS             complement(2719..3609)
                     /locus_tag="VIF08_05725"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8135521.1"
                     /translation="MTFQREFNCLWCGRLHRTRSDSDLEGYALLCPDCVGRAGENEFL
                     RFRLKGGLRLRAEAERPSAVAVAPVRTPDEELKVYYAQRAGEYDDWFLRKGRYSRGAI
                     ADAAWAADMDTATLWLDAQPLRGEIIELAAGTGWWSPLLAQKASTLWLYDINEEPLEL
                     ARQRLVAHGLAAHIHVRDAWAEPDRQVDGLFCGFWLSHVPRHRLGDFLALCRRWLKPG
                     GRMAFIDSRRDPESSAIDHPPPANDVSVRKLDDGQTFTIVKVYWQPEELEAALRTAGF
                     ASAHVTTTSRFFLMGEASAA"
     gene            complement(3606..4547)
                     /locus_tag="VIF08_05730"
     CDS             complement(3606..4547)
                     /locus_tag="VIF08_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY8135522.1"
                     /translation="MRIVSLLPSATEIVCALGLGDELVGRTHECDYPADVANVPVMTA
                     DVGGLPNATSREINDRVAHSIHGGSSIYALDLAALEAADPDLILTQELCSVCAVSYRE
                     VSDAVRRLDSDATVISLEPTSIEGIFNTISTVGAMAEAEDEAVGLVEMLRERLARIEN
                     RVLERRLQGIAARRVVCLEWLDPPFASGHWVPEQVRRAGGWDLLGALGQPARESTWMD
                     VRDVEPEQLLLMPCGFDAPRTADEWLRGRATRPDWIESLAAVRNGEVYALNGSAYFSR
                     PGPRVVDGIALLAELFDPREFTSEAPADGWLPVEVAA"
     gene            4611..5552
                     /locus_tag="VIF08_05735"
     CDS             4611..5552
                     /locus_tag="VIF08_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8135523.1"
                     /translation="MSEAGATASPVVVEGVWKRFGTTDVVADLSFDVRPGELLGFLGP
                     NGAGKTTTMRMILDIIRPDRGKISIFGMAPGLEHQGRVGYLPEDRGLYRDVPALDTLA
                     YFGALRGLSLRAARQRAKELLIEVGLGDSLTKKAGELSRGMHQKAQLIATIINSPDLL
                     IVDEPFQGLDPVNATMLKNLLLEQRDRGAAVVMSTHDMEDAQLLCDRILLIDRGKRVL
                     YGKVDDVRRAFSDGAVEVVGRHLPTDTDELKAATHANAFDGSVRYLLREGATAQDLFR
                     ELGDTDAEVERFAVEAPNLAEIFVRAVAGDERAQKVA"
     gene            5563..6810
                     /locus_tag="VIF08_05740"
     CDS             5563..6810
                     /locus_tag="VIF08_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8135524.1"
                     /translation="MRRSFLRLVLLVAQRDYLRTVRRRGFIAGTLLLPAAMALMFGIS
                     AFFSTAGSQQPLGQVLVVDESPVVLTTDQALTPHILLVDRADADARLAAGTARDYYLV
                     PAGWPAAPDIRRIVADGAGSNKPLSSIEALRLEQSAQSEIELMLRLSILRDAGLPDSA
                     LAQLLTPTRVTSVSTTGDPVTDASVLAGFALPYIFTLIFVLSIFITSGYLLQSVTEEK
                     ENRVVEIVLSSIPALPLMAGKVLGLGAAGLTQVAIWLVTALIAIPLINQQFSLDVSLS
                     PVQLVLAVAFFCLGYLGYGAIFAAVGAVAPGAREGQQYSSFFGFFAVVPVIATPLFVS
                     DPSSVICWALALFPLTAPAASLELLALPEVPWLMVGASFVAQLVFVIVATVLAGRIFR
                     ATLLLYGVRPSLGRIVGALTGRA"
     gene            complement(6840..7130)
                     /locus_tag="VIF08_05745"
     CDS             complement(6840..7130)
                     /locus_tag="VIF08_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEY8135525.1"
                     /translation="MTYVIAEPCIDVIDQSCVSVCPVDCIHFDQGVDRMLFIDPDECI
                     DCGACEPECPVNAIFPEDSLPTEWAAYTELNARWFKDKPSARAGVDELKPPA"
     gene            complement(7188..7742)
                     /locus_tag="VIF08_05750"
     CDS             complement(7188..7742)
                     /locus_tag="VIF08_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135526.1"
                     /translation="MAGQHLEAPDPPVLRPVSRWPVVVLIVGLSLALALVILGADLAG
                     RPVESPPIGTPGVVASPRVVTIIMRDYKFDPTPLYLYPGETVRLNVVNAGMVEHELVL
                     GDPAVQAAWAAADAGATPPAPFTTAPPASAPADTGGLRVLLESGGSASVLYVVPEGGE
                     LLLICHLAGHAERGMVGQVVRVER"
     gene            complement(7785..7952)
                     /locus_tag="VIF08_05755"
     CDS             complement(7785..7952)
                     /locus_tag="VIF08_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135527.1"
                     /translation="MNTVLTRLLQPTWLVIGLALLSLLVALAMLILAGGASDTEQLVG
                     PFRWEPLDLSA"
     gene            complement(8108..8494)
                     /locus_tag="VIF08_05760"
     CDS             complement(8108..8494)
                     /locus_tag="VIF08_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HEY8135528.1"
                     /translation="MSYTDRSITCADCGQEFTFTAGEQEFYAQRGFTEPPKRCPSDRA
                     LRKAQRGGAGNGGDRYGASSYDGGYSSSFAGAPAGYSSGGGYRERRPRELFEAVCADC
                     GKTASVPFRPTGNKPVYCSDCFQNHR"
     gene            8648..9265
                     /locus_tag="VIF08_05765"
     CDS             8648..9265
                     /locus_tag="VIF08_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY8135529.1"
                     /translation="MSERLVTRGFVGRRGSLDRGNRTPPGQYLTTDFPVLSAGPTPHT
                     PLDQWSLRLEGLVTKPVEWSWAEFNALPAREYVVDISCVTKWTKLDMSWHGVSVDTIL
                     AAVDLDPRAAFVIAFCDGGYTTNLPIADIVNDQAFIAYGYDGAPLAPEHGGPARLVVP
                     GRYFWKSAKWIRGLRIVEKDVPGFWEGFGYHNRGDQWLEERYSGD"
     gene            9270..10007
                     /locus_tag="VIF08_05770"
     CDS             9270..10007
                     /locus_tag="VIF08_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEY8135530.1"
                     /translation="MAGLAWEIATVAALNDETPHIRTLSLNVAGWSGHRPGQHVDIRL
                     TAEDGYQAQRSYSVASAPGGDGRIELTVERVAGGEVSPFLHDELIVGDRFEVRGPIGG
                     YFVWDGGSPDPLLLVAGGSGVVPLMAIVRHRAAIGSAAPCTLLFSSRTPADIIYASEL
                     ESLAARGDGLRVVHTLTREQPEGWAGYSRRIDAQMLEDVLGLTGKSASVFVCGPTPLV
                     ELAATSLVSLGLPPSRVRTERFGPSGG"
     gene            10009..10269
                     /locus_tag="VIF08_05775"
     CDS             10009..10269
                     /locus_tag="VIF08_05775"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEY8135531.1"
                     /translation="MDEHTLDANAVAGTLHEVFGVELTGQESECAHCGNHAQVGTFRV
                     YDMHGPGVVLRCSTCAQIVIRLVRRPDGSYLVDASGVYLKAT"
     gene            complement(10276..10449)
                     /locus_tag="VIF08_05780"
     CDS             complement(10276..10449)
                     /locus_tag="VIF08_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEY8135532.1"
                     /translation="MAIEIDPVCGMEVNTETGQFSTEHDGKTYWFCSRGCMLDFQEDP
                     DKYTDPNYQPEGM"
     gene            10481..11305
                     /locus_tag="VIF08_05785"
     CDS             10481..11305
                     /locus_tag="VIF08_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY8135533.1"
                     /translation="MADTGEPVSQARRPQGKPTEDRKLLRRAPYADTSFLDTDAWRAL
                     RIMGEFVEGFDSLAELSTAVSIFGSARIAQDHPYAVAAEDLAGKLARQGITVITGGGP
                     GIMEAANRGAARAGGVSVGLGIELPHEQAVNEWCNLVLNFRYFFARKTMFVKYAQAFV
                     IFPGGYGTLDELMESVTLVQTGKIDHFPVILYGTAYWQGLVDWLADPVVGEKMIAPED
                     LKLLRLTDDTDQVLKWILEAYAAAEEARVETLVSPKGPEHAEAQQEQSHQRHRRPD"
     gene            11405..11848
                     /gene="rpiB"
                     /locus_tag="VIF08_05790"
     CDS             11405..11848
                     /gene="rpiB"
                     /locus_tag="VIF08_05790"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEY8135534.1"
                     /translation="MKVALATDHAGLPLKEPIADWLRNAGHEVVDFGVNDPAPVDYPD
                     VIAPAARAVANGDCERGIVLGGSGTGEQIVANKVRGIRCVEAADPVTARLGREHNDAN
                     VLAMGARIIGREVALACVAAFMGGEFDGGRHARRVDKISALEDAF"
     gene            complement(11871..12782)
                     /locus_tag="VIF08_05795"
     CDS             complement(11871..12782)
                     /locus_tag="VIF08_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135535.1"
                     /translation="MSAADHALVSTNVAFAPPLDLAASLAPLGRAGDDLIDRFDGLRA
                     LRTLRLTPADDALAYAAEITDGSDRPALTILVPSDHAHILAALAQAVRATLLTERLAL
                     AQLAALDEAVARLARCYPGIVPVLYRDPFTALIRSISAQQVNLRWAATVRARLAQRYG
                     TRHSIGVWEVWTLHPEPLADARLEDLRALQLTTAKARAVIEVARAARAGELQLDQLDA
                     LDDEGLIAHLTGLRGIGRWSAEWFLARTLGRPRVVAGDLGVRKAIGRLYDMPVLPTEL
                     EVRRAAAHWGAAATHAQALALHDLAQP"
     gene            12800..13321
                     /locus_tag="VIF08_05800"
     CDS             12800..13321
                     /locus_tag="VIF08_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135536.1"
                     /translation="MANRIVAGAIAGAAGVVALNAVGYLDMLLRGRPASDLPARVAGK
                     LADEMGLPLDLSSDADVDADDEEVAELGDEDEPDAPANRREALGALLGMANGIAIGVC
                     YGVARLILPKPPAWLAGTALGALAMAASDYPATRLGLTEPRDWTATDWASDVLPHMAF
                     GVVTAIAFEAARD"
     gene            complement(13368..13541)
                     /locus_tag="VIF08_05805"
     CDS             complement(13368..13541)
                     /locus_tag="VIF08_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135537.1"
                     /translation="MTDDHHDVVVTDGGSSVGALLGIILAIVIVLAIVWFFFLSGGQQ
                     SAPDINIIQPPAS"
     gene            13664..15238
                     /locus_tag="VIF08_05810"
     CDS             13664..15238
                     /locus_tag="VIF08_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY8135538.1"
                     /translation="MTGPRRERLGGLWWQRGVVYQIYPRSFQDSNGDGVGDLVGIRRR
                     LDHLEWLGVDAVWLSPIFRSPMADFGYDVADYRDVDPLFGTLANLDELIADMHRRGMR
                     LLLDYVPNHTSAQHPWFVDSRSARDAEHRDWYIWRDPSPGGGPPNAWQSAFLGSAWEW
                     DARTGQYYFHSFLKEQPDLNWTNPEVRAAMKAVMRYWFERGVDGFRVDVVNLLAKGPE
                     LASPDHPDEHDKWGDEPAVHPLMRELRAVADEFDERVLIGEIWLPPSELVTFYGEALD
                     ELHLPFNFGLLELKEWGAETVCNAVVAYEKAMPPRAWPNWVLGNHDRRRMATRLGAAQ
                     ARVATMMLLTLRGTPTLYYGDEIGMVEADLPRSVWRDPQGLRGGPTRDGCRTPMIWDA
                     TALGGFTTGAPWLPLDQPAGVDVATERDDPGSMLSLTRSLLELRRAEPALNVGEWRDL
                     GHAGSAIAYIRSSDAPDGRRFLVCLNLASKPAPLPAHATSLRGEIVVSTLPGGAGERF
                     EGRDLAPDEGLVICLG"
     gene            complement(15257..>15449)
                     /locus_tag="VIF08_05815"
     CDS             complement(15257..>15449)
                     /locus_tag="VIF08_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135539.1"
                     /translation="QLIQRLRDVHPQIPIVLTAPGVEFDWVETDGSHGVVALEGALSS
                     AMLRQAVVRALESVGRAPD"
BASE COUNT         2393 a         5277 c         5322 g         2457 t
ORIGIN      
        1 cagcgccacg aggcgctcat gcaggtcacg ccggacgccg tagagcatct ggaactcgta
       61 gcgcgccggg tcgatggcgt gctccatgac gaacgcgagc gcgtgatcga tgagccgatc
      121 gtcgtgcgtc gccagggccg ggtagttgcc gtccgtgagc agccgctcca tcagctcgat
      181 atacgagcgg tcgacctctt cgcgcgtcct gaaggcgacg ctcgccggtt cgtcgtacgc
      241 gcccttgcac aggcggacgc ggatgccctc cgcgttgagc gccgcgacgt cgtcggccga
      301 gcggcgcagg taagactgga tgaccgcgcc gacgccctga tagtcggcgt gaagctcgcg
      361 gacgatctcg agggtcttag ttgtcttggt gtggtcctcc atgtcgatgc gcacgaaggc
      421 cccgcccgac cgggcacggt ccacgatgcg ccgcacgttg gctaggcaga agtcgcgatc
      481 gatggccagg cccatctgcg tcagcttcac gctgacgttg gcctcgaggc cgcgttccgc
      541 cagcgcgtcg agggtctcga tgtagcgatc cgcggccgcc gtcgccatcg cctccgacga
      601 gatggactcg cccagcacgt cgacggtcca gcgcatgcct gcggcctgga gaccctcgag
      661 cgtggccagc gcgtcgtcga gacgctcacc ggggacaaag cgtcggacca tgcgccgcgt
      721 cagcggaatc gcggtggcca gccggcccag gaagcggcgg tgcgacgccc acaggaaaaa
      781 cgcgcgggac agcttcgcgg gcagggcgcg cggatggaac ttcgcggcct catgcgcggc
      841 cgcttcctct tcgcgccgtt cgacctcggc cgcgatcacg gtcgcgggga tgacgccgtc
      901 gagcgagcca tcgggctggc ggtacagccg gcaacccagg tggaacccct cgccggccgg
      961 cacagggacg acgtcgcggc cgtcgatgcg caaggtcggt gacccgtgga agcccaggcc
     1021 tgccgcggtg tcgatgtcgc tgacgtacac gcgctcgatg gccggcgagt ggcccaagcg
     1081 accatcgttg gccagggcct cgcgcgcgat ctcctcggcc tgttcgaggt gtgggcagtc
     1141 cggggtcgcg aggagctcga cgcgcatcag gtgtcgctcc gcatcacgtg tcgctcacgc
     1201 cgccgattac cgccacgggc tccttgtaac agccactcac aagtgcgatc aagagcagtc
     1261 cggcccgtcc tgtcttacgc gctggtccac tgaggctcat caacccttgg aggtgtcgat
     1321 ctccagcgtt cggcccagct cctcggtccg ccgaaacgcc gcccacaccg cctcgctgag
     1381 gacagtcctg aggcggcccg actcgagctc gtgcagcgcg gccgcgctcg tgccgccggg
     1441 cgatgtgacc atgttgcgca gctgcgccgg gtgcatgccc gacgacttgg caaacagcgt
     1501 gctgccctcg agcgtctcga tcacgaggtc atgtgcgacg tggcgcggga agcccaggtg
     1561 gaccgccgcg tcgatgagcg cctccatcac caggaagacg taggtcgggc cagtgccact
     1621 gacggcggta gccatcgcaa cgagcttctc gtcatcgacc tcgagctgca cgcccaggct
     1681 gcccagcagg acgcgcgcct ggtcgcgctg ggtcttcgtc accctgggtg tcgcgtacca
     1741 gactgtcatg cctcggccaa gctgggccgg ggtgttgggc atgctgcgca cgatctggtc
     1801 gtggcctagc gcgttttcca gggcggtcgt cgtcgcgccg gcgatgatgc tgatgacgag
     1861 cttgccgcgt ttcagcgccg gcgcgatctc cctgccgacg cgggcaagca tctgtggctt
     1921 gatcgcgagc acgacaacgt ccgccttgtc gaccgcgctg gcgttgtcct cgaccgtgct
     1981 gatcccgtat gagctgatca gctcgcgccg ccgatcggcc cgcggatggg tcgcgatgat
     2041 ccgattcggt gcgacctgct tgccgcgcaa taggccggcg atcatcgcct ccgccatcac
     2101 cccggagccg acagttgcga tcgtggacct ggcgagcgac atggcggcga gtttagtgcc
     2161 gccggcactg acggctttgg ccagatgact acgtcttggc cgcctggtcg accagtttct
     2221 tctcgatgcg cgtcctctcg ccgatcctgc gccccagcgc ttccgcctgt gcggcaagcc
     2281 gctgaagctc ctcgtcgttg gctttgggat cctgtttccg gcgctccacg tgagccagca
     2341 actgtgcgtc ggcctcgatc tcgtcgctgg tcgtccggat atcctcgcgc acgctgtccg
     2401 acacgtagcc ctcccgctcc cccgtactag tcagccaccc agtggccatc gcgatagacg
     2461 taccaaacct gagcccgctc gccctcgagt gaccgctgcc tgacgatcac gttggggtag
     2521 tgcctcgcca gccgcgaacg aagttcctcc gggctcgtgg ccgatgcgga ttcaacagtc
     2581 accatctcca cgaacgcggc gtctcgcggg ggattgatga agagcgtcgg cggcaccagg
     2641 gcgaatggtc aggcatgggc gtttgcgcca attctgtcgc ggcggcagtc gcgatttcgt
     2701 ccccgtaaca aacgcatgtc aggccgcgct ggcttcgccc atcaggaaga agcggctcgt
     2761 ggtcgtgaca tgcgccgacg caaaaccggc ggtgcggagg gccgcctcga gctcctcggg
     2821 ctgccagtac accttgacga tcgtgaatgt ctggccgtca tcgagcttgc gcacgctcac
     2881 atcattggcc ggcggcggat ggtcgatcgc gctcgactcg gggtcgcggc gtgaatcgat
     2941 gaaggccatc cgcccgccgg gtttcagcca gcgccggcaa agggcaagga agtcgccgag
     3001 tcgatggcgc ggcacgtggc tgagccagaa gccgcagaag agcccgtcca cctggcggtc
     3061 cggctcggcc cacgcgtcgc gcacgtggat atgcgcggcg agcccgtgcg cgaccaggcg
     3121 ctggcgcgcg agctcgagcg gctcctcgtt gatgtcgtag agccacaacg tcgaggcttt
     3181 ctgggctagc agcggcgacc accagccggt gccggcggcc agctcaatga tctcgccgcg
     3241 cagtggttgc gcgtccagcc acagggtggc cgtgtccatg tctgcggccc acgccgcgtc
     3301 ggcgatggcg ccgcgcgaat agcggccctt gcgcaggaac cagtcgtcgt attcgccggc
     3361 gcgctgcgcg taatagacct tgagctcctc gtcaggggtg cgcaccggag ccacggccac
     3421 cgctgacggg cgctccgcct ccgcccgcag acgcaggcct cccttcagcc ggaagcgcag
     3481 gaactcgttc tcgcctgccc ggccgacgca gtcgggacag aggagcgcat agccctcgag
     3541 gtccgagtcg gatcttgttc ggtggagccg gccgcaccag aggcagttga attcgcgctg
     3601 aaacgtcaag cggcgacctc gaccggcagc cagccatccg ccggcgcctc ggacgtgaac
     3661 tcgcgcgggt cgaacagctc ggccaggagg gcgatgccgt cgacgacgcg tggtcccggc
     3721 cgggagaagt acgccgatcc attgagcgcg tagacctcgc cgttgcgcac cgcggcgagg
     3781 ctctcgatcc agtccggccg ggtggcgcgg ccgcgcagcc actcatcagc agtccgggga
     3841 gcgtcaaagc cgcacggcat caacagcagc tgctcgggct cgacgtcgcg cacgtccatc
     3901 caggtcgact cgcgtgccgg ctggccgagc gcgcccagca ggtcccagcc acccgcgcgg
     3961 cgcacctgct caggcaccca gtggccgctc gcgaagggcg ggtcgagcca ctcgagacac
     4021 acgacgcgcc gcgcggcgat gccctgcagc cggcgctcga gcacgcggtt ctcgattcgc
     4081 gccagccgct cgcgcagcat ctccacgagg cccaccgcct cgtcctccgc ctcagccatg
     4141 gcacccacgg tcgagatggt gttgaagatg ccctcgatgg acgtcggctc gaggctgatg
     4201 accgtcgcgt cggaatccag gcgccgtacc gcatcggaca cctcgcgata ggacaccgcg
     4261 cagaccgagc acagttcctg ggtcaggatc aggtccggat cggcagcctc cagcgcggcc
     4321 aggtccaatg cgtagatgga tgagcccccg tggatcgagt gggcgacgcg gtcgttgatc
     4381 tcgcggctgg tggcattggg cagcccgccc acatcggcgg tcatcaccgg cacgttggcc
     4441 acgtcggccg ggtagtcgca ctcgtgcgtc cggccgacaa gctcgtcgcc caggcccagg
     4501 gcgcacacga tctccgtggc cgacggcagc agcgaaacga tgcgcatgac cttcaggata
     4561 ggccgagtgt cacttgcgcc ctccacggcc cacttcgcta ggctggcagg gtgagcgagg
     4621 ctggagcgac cgcgtcaccg gtcgtggtcg agggggtctg gaagcgcttc ggcaccacgg
     4681 atgtcgtggc cgacctgtcg ttcgatgtcc gacccggcga gctacttggc ttcctggggc
     4741 cgaacggcgc gggcaagacg acgacgatgc gcatgatcct ggacatcatc cggcccgatc
     4801 gcggcaagat cagcattttc ggcatggcac cggggctgga gcaccagggt cgcgtcggct
     4861 acctgccgga ggaccgcggc ctgtatcgcg atgttccggc gctcgacacg ctggcctact
     4921 tcggcgcgtt gcgcggcctc tcactgcgcg cggcccggca gcgcgcaaag gagctgctca
     4981 tcgaggtcgg cctgggcgac tcgctcacca agaaggccgg cgagctgtca cgcggcatgc
     5041 accagaaggc acagctcatc gcgaccatca tcaactcgcc cgacctgttg atcgtcgatg
     5101 agccgttcca gggcctcgac ccggtcaacg ccacgatgct caagaacctc ctgctggagc
     5161 agcgcgaccg tggggcggcc gtcgtcatga gcacgcacga catggaggac gcgcagctct
     5221 tgtgcgaccg catcctgctc atcgaccgcg gcaagcgcgt gctctacggc aaggtggacg
     5281 acgtgcgccg ggcattcagt gacggcgcgg tcgaggtggt gggcaggcac ctgccgaccg
     5341 ataccgacga gctgaaggcg gccacgcacg cgaatgcctt cgacggctcg gtgcgctacc
     5401 tgctgcgtga gggcgcgacc gcgcaggacc tgttccgcga gctgggcgat accgacgcgg
     5461 aggtcgagcg cttcgccgtg gaggcaccaa acctggctga gatcttcgtg cgtgccgtcg
     5521 ccggcgacga gcgggcgcag aaggtcgcgt gaggagctga ccatgcgccg ttccttcctg
     5581 cgcctcgtcc tgctcgtcgc ccagcgcgac tacctgcgca cggtgcgccg gcgcggcttc
     5641 atcgccggca cgctgctgtt gccggcggcg atggcgctca tgttcggcat ctcggctttc
     5701 ttttcgaccg cgggctcaca acagcccctg ggccaggtgc tcgtggtcga cgagtcgccg
     5761 gttgtcctga caaccgacca ggcgctgact ccgcacatct tgctggtcga tcgcgccgac
     5821 gcagacgcac ggctcgcggc aggcacggca cgcgactact acctcgtgcc cgccggctgg
     5881 ccggccgccc ccgacatccg acgcatcgtc gccgatggtg ccggctcgaa caagccattg
     5941 agctcgatcg aagcgctcag gctggagcag tccgcccagt cggagatcga gctgatgctg
     6001 cggctatcca tcctgcgtga cgctggcctg cccgactccg ccctcgcgca gctgctcaca
     6061 ccgacccgtg tcacgtcagt ctcgacaacc ggcgacccgg tcactgacgc gtcagtcctg
     6121 gccggcttcg cactgcccta catcttcacc ctgatcttcg tgttgagcat cttcatcacc
     6181 agtggctacc tgctccagtc cgtgacggag gagaaggaga atcgagtcgt agagatcgtg
     6241 ctcagctcga tcccggcgct gccgctgatg gccggcaagg tgctcgggct gggggcggcc
     6301 ggcctgaccc aggttgcgat ctggctcgtc acagcgttga tcgccatacc tcttatcaat
     6361 cagcagttct cgctcgacgt atcgctgtca cctgtccagc tggtcctggc ggtggccttc
     6421 ttctgcctgg ggtacctggg ctacggtgca atcttcgccg ctgttggcgc cgtggcaccg
     6481 ggcgcccgcg agggccagca gtactcgagc ttctttggct tcttcgcagt cgtgccggtc
     6541 atcgccacgc cgctcttcgt gagcgatccc agctcggtga tctgttgggc gctggcactc
     6601 ttcccgctga ccgcgccggc ggcgtcgctg gagctgctgg cgctgcccga ggtgccgtgg
     6661 ttgatggtcg gcgcgtcgtt cgtcgcccag ctggtcttcg tgatcgtggc cacggtgctg
     6721 gcggggcgga tcttccgcgc gacgctgctg ctctatggcg tcaggcccag cctcggccgg
     6781 atcgtgggcg cgctgacggg ccgcgcctaa cctcatcgcg gccggttacg gccgtcgaat
     6841 caggccggtg gcttgagctc gtcgacaccc gcccgcgccg acggcttgtc cttgaaccag
     6901 cgcgcgttga gctccgtgta cgcggcccat tcggtgggca gcgagtcctc agggaagatc
     6961 gcgttgaccg ggcactccgg ctcgcacgcg ccgcagtcga tgcattcgtc gggatcgatg
     7021 aacagcatcc gatcgacgcc ctggtcgaag tggatgcagt cgaccgggca gaccgagacg
     7081 cacgactggt cgatgacgtc gatgcagggc tcggcgatga cgtaggtcaa ggactggtct
     7141 cctcgggaat tggcagctgc tctggggtga tcaggtggtg cgtcatctta gcgctccacg
     7201 cgcacgacct gcccgaccat gcctcgctcg gcgtggcccg ccaggtggca gatcaaaagg
     7261 agctcgccac cttcgggaac gacatacagg acgctcgccg agccgcccga ctcgagcagc
     7321 acgcgcagcc cgccagtgtc ggccggcgcg ctcgcgggcg gcgcggtcgt gaacggcgcc
     7381 ggcggggtcg cgccggcgtc agcggccgcc caggccgctt ggacagccgg gtcacccagc
     7441 acgagctcgt gttcgaccat gccggcgttg accacgttca gccgaaccgt ctcgcccggg
     7501 tagaggtaaa gcggcgtcgg atcgaacttg tagtcgcgca tgatgatcgt gaccacgcgc
     7561 ggcgaggcga cgacgcccgg cgtcccgatg ggtggcgact ctaccggccg ccccgcaagg
     7621 tcagcaccca ggatgaccag cgccaaggcc aggctgagac cgacgatgag aacgacgacc
     7681 ggccaccgtg agactggccg caatacaggc ggatcgggcg cttctaagtg ctggccggcc
     7741 acctcgtgac tgtaggcgcg cggccggcac cacgcagccg ggcgtcaggc ggagaggtct
     7801 agcggctccc agcggaatgg cccgacgagc tgctctgtat cgctcgcgcc accggccagg
     7861 atgagcatcg ccagcgctac gagcagggac agcaaggcaa ggccgatcac aagccaggtc
     7921 ggctgaagaa gacgcgtaag gactgtgttc atggctctcc gatcgctgcc tgatgtgggg
     7981 cgggaccaac cgccggcgac tctaggcagc gccgcttatc gggcgctttg cgcccaattc
     8041 atcggaggag tcggcgcgct gtggataaga cgcgccgtgc gattgacgaa cgagctcggc
     8101 gagcgagcta gcggtggttc tggaagcagt cgctgcagta gaccggcttg ttgccggtcg
     8161 ggcggaaggg cacgctggcc gtcttgccgc agtcggcgca gaccgcctcg aacagctcgc
     8221 gcggccgccg ctcgcggtac ccgccgccac tcgagtagcc cgcgggcgcg ccggcgaacg
     8281 aggacgagta gccgccgtcg tatgacgacg cgccatagcg gtcgccgccg ttgccggcgc
     8341 cgccgcgctg ggccttgcgc agcgcgcgat cagatgggca gcgcttgggc ggctccgtga
     8401 agccacgttg ggcgtagaac tcctgctcgc cggcagtgaa ggtgaattcc tggccgcaat
     8461 cggcacaggt gatcgaacga tccgtgtacg acaccctaga agaagcctcc gaccgatacg
     8521 agttgtttcc ctctccccgc gctgccctcc ctgcggcagc acgagtcggc gggaatatag
     8581 acgtgcggtc aagcgtggcg caacgaggtg tcgcgttcgg gtaccacgcc gggtaacctc
     8641 cgcgaggatg agcgagcgcc tcgtgacgcg cggttttgtg ggtcggcgcg ggtccctgga
     8701 ccggggcaac cgcacgccgc ccggccagta cctgaccacc gacttccccg ttttgtcggc
     8761 cgggcccacg ccccatacgc cgctggacca gtggtcgttg cgtctcgagg gcctcgtcac
     8821 caagccggtc gagtggtcgt gggccgagtt caacgcgctg cctgcgcgcg agtacgtcgt
     8881 cgacatcagc tgcgtcacca agtggaccaa gctcgacatg agctggcacg gcgtcagcgt
     8941 cgacacgatt cttgcggcgg ttgacctcga tcccagggcc gcgttcgtga tcgccttctg
     9001 tgacggcggc tacacgacca acctgccgat tgccgacatc gtcaatgacc aggcgttcat
     9061 cgcgtacggc tatgacggcg cgccgctcgc gccggagcac ggcgggccgg cacgcctcgt
     9121 tgttcccggc cgctacttct ggaagagcgc caagtggatt cgcgggctgc gcatcgtcga
     9181 gaaggacgta cccggcttct gggagggctt cggctaccac aaccgcggcg accagtggct
     9241 cgaagagcgc tatagcggcg actgaactga tggccggcct tgcctgggag atcgcgacgg
     9301 tcgcggcgct caacgacgag acgccccaca tcaggacgct ctcgctcaac gttgccggct
     9361 ggagcgggca tcgtcccgga caacacgtcg acatccggct caccgcggaa gacggctatc
     9421 aggcccagcg cagctattcc gtcgcgtcgg cgcccggcgg cgacggccgg atcgagctga
     9481 ccgtcgagcg cgtggccggc ggtgaggtct cgccttttct gcacgacgag ctcatcgtgg
     9541 gggatcgatt cgaggtgcgc ggtcccatcg gcggctactt cgtctgggat ggcgggtcgc
     9601 ctgacccact gctgctcgtc gccggcggct cgggcgtcgt gccgctcatg gcgatcgtcc
     9661 gtcaccgcgc ggctatcggc tcggccgcgc cgtgcacgct gctcttcagc tcacgcaccc
     9721 cggccgacat catctacgcc tcggagctgg agtcgctcgc ggcgcgcggc gatgggctgc
     9781 gcgtcgtcca cacgctcacg cgggagcagc cggagggctg ggcggggtac tcacggcgca
     9841 tcgacgccca gatgctcgag gacgtcctgg gcctcaccgg caagagcgcc agcgtcttcg
     9901 tgtgcggccc cactccgctc gtcgagctcg cggccacgag cctcgtcagc ctggggctgc
     9961 cgccgagccg cgtgcggacc gagcgcttcg gaccatcggg aggctgacgt ggacgaacac
    10021 acactcgacg ccaacgcggt ggcgggcaca ttgcacgagg ttttcggcgt cgagttgacc
    10081 ggccaggaga gcgagtgcgc gcactgcggc aaccacgccc aggtcggcac cttccgcgtc
    10141 tacgacatgc atggcccggg cgtggtcctg cgctgcagca cctgcgcgca gatcgtcatc
    10201 cggctcgtcc gccgcccgga cggcagctac ctggtcgacg ccagcggggt ctatctcaag
    10261 gcgacgtaga gggctctaca tgccctcggg ctggtagttc gggtcggtgt acttgtcggg
    10321 gtcttcctgg aaatcgagca tgcagccgcg cgagcagaac cagtacgtct tgccgtcgtg
    10381 ctcggtgctg aactgtcctg tctctgtgtt gacttccatg ccgcacacgg gatcgatctc
    10441 aattgccatg cgtctattct gcgaccgatg gagatagagc gtggctgaca cgggcgagcc
    10501 ggtgagccag gcacgccggc cgcagggcaa gcccacggaa gatcgcaagc tcctgcgccg
    10561 cgcgccgtac gcggacacga gcttcctgga cacggacgca tggcgggcgc tgcgcatcat
    10621 gggcgagttc gtcgagggct tcgactcgct cgccgagctg agcacggcag tgagcatctt
    10681 cggctcggcg cgcatcgcac aggaccaccc ctacgcggtc gcggcggagg acctagcggg
    10741 caagctcgcc agacagggca tcacggtcat caccggtggc ggcccgggga tcatggaggc
    10801 cgccaaccgc ggcgctgcca gggcgggcgg cgtgtcggtc gggctgggca tcgagctgcc
    10861 gcacgagcag gcagtcaacg agtggtgcaa cctcgtgctc aacttccgct acttcttcgc
    10921 gcgcaagacg atgttcgtga agtacgccca ggcattcgtc atctttcccg gtggctacgg
    10981 cacgctcgac gagctgatgg aatcggtgac gctcgtccag accggcaaga tcgatcactt
    11041 cccggtgatc ctctacggca ccgcgtactg gcagggcctg gtggactggt tggccgatcc
    11101 ggtcgtcggg gaaaagatga ttgcccccga ggacctgaag ctgttgcgcc tgaccgacga
    11161 caccgaccag gtgctgaagt ggatcctgga ggcgtatgcc gcggccgagg aggcgcgcgt
    11221 ggagacgctg gtcagcccca agggaccaga gcacgccgag gcgcagcagg agcagagcca
    11281 ccagcgccac cggcgacccg actagccaca aggaggcgcc ggggcatcgg cattcatgcg
    11341 gacgcaccca gccgacgcgc agcagcggcc cgcaggccgg ctgcggaggt aatacggttc
    11401 agccatgaag gtcgccctgg ccaccgacca cgccggactg cccctgaagg agccgatcgc
    11461 cgactggctg cgcaacgccg gccacgaggt cgtcgacttc ggcgtgaacg acccggcccc
    11521 ggtcgactac ccggacgtga tcgcaccggc ggcgcgggct gttgccaacg gtgactgcga
    11581 gcgtggcatc gtcctcggcg gcagtggcac gggcgagcag atcgtcgcca acaaagtgcg
    11641 cggcatccgc tgcgtggaag ccgccgatcc ggtcaccgcg cgactgggcc gcgagcacaa
    11701 cgacgcgaac gtgctcgcca tgggggcgcg gatcatcggg cgcgaggtgg cgctggcctg
    11761 cgtggcggcc ttcatgggcg gcgagttcga cggtggccgg cacgcccgcc gggtggacaa
    11821 gatcagcgcc ctggaggacg ccttctagcg ccgcgcgcgg tcgcggcgac tcagggctgt
    11881 gcgaggtcgt gcagggcgag cgcctgggca tgcgtcgcgg ccgctcccca gtgggcagcc
    11941 gcgcggcgca cttcgagctc cgtcggcagc acgggcatgt cgtacaggcg accgatggcc
    12001 ttgcgcacac cgagatcgcc ggccacgacg cgcggcctgc ccagcgtccg cgccaggaac
    12061 cattcggcac tccagcggcc gatgccgcgc aggccggtca gatgggcgat caacccctcg
    12121 tcatcgagcg cgtcgagctg gtcgagctgc agctcgccgg cccgcgcggc ccgcgcgacc
    12181 tcgatcacgg cgcgcgcctt ggccgtagtc agctgcagcg cgcgcaggtc ctcgaggcgc
    12241 gcgtcggcga gcggctccgg gtggagcgtc cagacctccc agacaccaat cgagtgccgc
    12301 gttccgtaac gctgcgcgag tcgcgcgcga accgtcgccg cccagcgcag gttgacctgc
    12361 tgcgcgctga tcgagcggat gagcgccgtg aacggatcgc ggtacagcac cggcacgatg
    12421 cccgggtagc accgcgccag ccgcgcaacc gcctcgtcga gagcggccag ctgagcgagc
    12481 gccaggcgct cggtcaacag cgtcgctcgc acggcctgcg ccagggccgc aaggatgtgc
    12541 gcgtggtcgg acggaacgag aatggtcaga gcgggtcgat cggagccgtc cgtgatctcg
    12601 gcggcatagg cgagcgcgtc atccgccggc gtcaacctca acgttcgcag cgcccgtagc
    12661 ccgtcgaagc gatcgatcag gtcgtccccg gcgcgcccga gcggcgccag gcttgccgcg
    12721 aggtcgagcg gcggcgcgaa tgcaacgttc gtgcttacga gggcatggtc agcggcgctc
    12781 acagggctac gctataggca tggccaacag gatcgtcgca ggcgccatcg cgggcgcggc
    12841 cggtgtcgtc gcactcaacg ccgtgggcta tctcgacatg ctgttgcgcg gccgcccggc
    12901 aagcgacctg cccgcgcggg tcgccggcaa gctggccgac gagatgggcc tgccacttga
    12961 tctgagcagt gacgctgacg tagatgccga cgatgaggag gtcgcggagc tgggcgacga
    13021 ggacgagcct gatgcgccgg ccaaccggcg cgaggccctg ggtgcactgc tcgggatggc
    13081 caacggcatc gccatcggcg tctgctacgg ggtggcccgg ttgatcctgc ccaagccgcc
    13141 ggcctggctt gccggcacgg ccctcggcgc cctggcgatg gccgcctcgg actatccggc
    13201 gacgcgcctc gggctgaccg agccgcgcga ctggacggcg accgactggg cgagcgacgt
    13261 gctgccgcac atggcgtttg gcgtcgtcac cgccatagca ttcgaggcgg cccgcgattg
    13321 actcgcgcga ccgcctcgtg ccacgtgatt gatggtttgc cggcggacta ggacgccggc
    13381 ggctggatga tgttgatgtc gggggccgac tgctggccgc cactcaagaa gaagaaccaa
    13441 acgatggcca gcacgataac gatggccagg atgatgccca gcagcgcacc aacgctactg
    13501 ccaccatcgg taacgacaac gtcgtgatga tcgtcagtca tgtgaagtct ccttttccgg
    13561 cgcacacggc ggtgcggccg ctgaaagggt cgctggcaag cggttgcaag cataagaagc
    13621 gccctgtgcg gccctgctca caatttgctt gcaggcgctc ccgatgactg ggccgcggcg
    13681 cgagcggctg ggcggcctat ggtggcagcg cggcgtcgtc tatcaaatct atccacgttc
    13741 attccaggac tcgaacggcg atggcgtggg cgatctcgtc ggcatccgcc ggcgcctcga
    13801 tcacctcgag tggctgggcg tggacgctgt ctggctgtcg ccaatcttcc gatcaccaat
    13861 ggccgacttc ggctacgacg tggccgacta ccgcgacgtc gaccccctct tcggcacgtt
    13921 ggccaacctc gatgagctga tcgccgacat gcatcgccgg ggcatgcggc tgctgctcga
    13981 ctatgtgccc aaccacacgt ccgcgcagca tccgtggttc gtcgactcgc gatcagcgcg
    14041 cgatgccgag catcgcgatt ggtatatctg gcgcgacccg tcgcccggtg gcgggccgcc
    14101 caacgcgtgg cagagtgcgt tcctcggcag tgcatgggag tgggacgcgc gcacgggtca
    14161 gtactacttc cactcgttcc ttaaggagca gcccgacctc aactggacga accccgaggt
    14221 gcgcgcggcg atgaaggccg tgatgcgcta ctggttcgag cgcggcgtgg acggcttccg
    14281 cgtggacgtg gtcaatctcc tcgccaaggg ccccgagctg gcctcgcccg accaccccga
    14341 cgagcacgac aaatggggcg atgagcctgc cgtgcacccg ttgatgcgcg agctgcgggc
    14401 agtggccgac gagttcgacg agcgcgtgtt gatcggggag atctggctgc cccccagcga
    14461 gctcgtgacg ttctacggcg aggcactcga tgagctgcac ctgccgttca acttcggatt
    14521 gctcgagctc aaggagtggg gcgctgagac ggtatgcaac gccgtggtcg cttatgagaa
    14581 ggcgatgccg ccccgggcgt ggccgaactg ggtgctgggc aaccacgacc ggcggcgtat
    14641 ggcgacgcgc ctcggcgctg cacaggcgcg tgtggccacg atgatgctgc tgaccctgcg
    14701 cggcacgccg acgctctact acggcgacga gataggcatg gtcgaagctg acctgccgcg
    14761 ctcggtgtgg cgcgaccccc aggggctgcg cggcgggcca acgcgcgacg gctgccgcac
    14821 gcccatgatc tgggacgcga ctgcgctggg cggcttcacg acgggcgcgc cgtggctgcc
    14881 gctggaccag ccagctggcg ttgacgtcgc gacggagcgc gacgacccgg ggtcaatgct
    14941 gagcctgacg cgctcgttgc tggagctgcg gcgcgccgag ccggcgctga atgttgggga
    15001 gtggcgcgac ttgggccacg ccgggagcgc cattgcctac atccgcagca gcgacgcgcc
    15061 cgacggccgg cgcttccttg tctgtctgaa cctggccagc aaacccgcgc cactgcccgc
    15121 tcacgccacg tcgttgcgcg gcgagatcgt cgtctcaacg ctccccggtg gcgcgggcga
    15181 gcgcttcgaa ggtcgcgact tggcgcccga cgagggcctg gttatctgcc tgggctaatc
    15241 ggccccgtgg tcgccctcag tccggcgccc ggccgaccga ctccagtgcc cgcacgaccg
    15301 cctggcgcag catcgcggag ctcagcgctc cctcgagggc aacgacgccg tggctcccgt
    15361 cggtctccac ccagtcgaac tcgacgcccg gcgctgtgag gacgatcggg atctgcgggt
    15421 gcacgtcgcg cagccgctgg atcagctgt
//
LOCUS       DATBRX010000150         7246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_834897, whole genome shotgun sequence.
ACCESSION   DATBRX010000150 DATBRX010000000
VERSION     DATBRX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834897"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..458
                     /gene="mscL"
                     /locus_tag="VIF08_05820"
     CDS             57..458
                     /gene="mscL"
                     /locus_tag="VIF08_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY8135540.1"
                     /translation="MLKEFRDFLLRGNVIDLAVAVVIGGAFGAVVTSFVGDVLTPLLG
                     LIGVPDFSTLAITTPGGAVIAYGLFLNALISFVLIAAAIFFFVIKPLNSLEKRRQAGA
                     ETSPTTKTCTECASAIPLAARRCPNCTQPQP"
     gene            386..1591
                     /locus_tag="VIF08_05825"
     CDS             386..1591
                     /locus_tag="VIF08_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135541.1"
                     /translation="MHRVRIGHPAGGPALPELHPAAAVMGRRGWLRGASYAALVAVLA
                     AACSGGPKSPVGPVGPGGATLPPQAIGNAVLFDPWQITDPFSGGVAAVTYLVPTGWQS
                     SGQVVWMPAWQRLVDLQTIVSDPVSGATVEWLPIQDFIFGQAPAGLEPPIGGNYQGKM
                     WVPPVTDPVQFVSDFWMSGPLAHLADATLLSLEQVPAVADEFLLGFGGPANAAAYRMR
                     YSFTQNGQAWDEQVNFALLYASGGGIDSWYVNFAYTMRAPQGVLDQLAPVLSTIVSSR
                     DSTPQFQAIVTMTQRLFTQGIQQQMADTEAFGRLLAEQRAQTDALQAQITADRQASQD
                     RIAQLRGEVLSGVQTFSDPVNGRLVQLPAGQSTYWVNNQGQYFASDQPGFDPSTLGGG
                     WTQLQPRQF"
     gene            1594..2121
                     /locus_tag="VIF08_05830"
     CDS             1594..2121
                     /locus_tag="VIF08_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135542.1"
                     /translation="MVVARITPDEWALLRDMRLRALRDAPEAFGQTYENAAAEPELEW
                     RSAARAASAGDRRAWFFARSDGSAAEVRSAAGAPPAELGMVQARRRPPDDCLVFSMWV
                     APEARRGGVGRELIAAAADWARSWDGRRIVLWVTGVNESAMRFYERVGFRLLSEGPDM
                     ESGRAFGALAMELPL"
     gene            complement(2128..4119)
                     /locus_tag="VIF08_05835"
     CDS             complement(2128..4119)
                     /locus_tag="VIF08_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135543.1"
                     /translation="MSGQMPGRPQQGKGGPPAGPRGPRGGPMAMMMGGPPAEKSKNFG
                     LSFRRLMRQLAPERWLIVAVIALCAISVVLQVVGPKILGDATNIIFAGAIGQELPAGM
                     TQAQVEELLRSRGQGQFADMLHGMTLVPGAGIDFARLANILAVLVGIYLVASIFAWGQ
                     AYIMAGVTQRTVYRLRAAVDVKLGRLPLKYFDDHPRGDVLSRVTNDIDNIGQSLQQSL
                     TQLMTSVLTVIGVLIMMVTISWELALISLLAVPLSLVVSLLIIRRSQKQFVAQWASTG
                     ALNGQVEEAHTGHNIITVFGRQREAAARFATENERLYQASYRAQFISGIIQPALNVIG
                     NFNYVGIAVIGGLRVATGQMTLGDVVAFIQYSRQFSQPILQAASIANVLQSAVASAER
                     VFELLDEAEMVTDPADAPALGEVAGKVAFEDVSFRYLPDTPLIEHLSLTAKPGATVAI
                     VGPTGAGKTTLVNLLMRFYEIDSGRITIDGIDTRSVRREDVRREFGMVLQDAWLFNGT
                     IRDNIAYGREGATDAEIVAAAEKAHVDHFVRTLPGGYETVVDDDATNLSQGEKQLLTI
                     ARAFLADPRILILDEATSSVDTRTEVLIQRAMAKLMSDRTTFVIAHRLSTIRDADLIV
                     VMDHGRIVETGTHQQLLEAKGFYNELYQSQFVEALAAVA"
     gene            complement(4116..5858)
                     /locus_tag="VIF08_05840"
     CDS             complement(4116..5858)
                     /locus_tag="VIF08_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135544.1"
                     /translation="MIRLLRRYLGAYKGQIAIVLVLLLVQALTQLYLPDLNAEIINQG
                     VATGDSNLILNVGLVMVGVTIILAITSLVAVYYSARVAMGFGRDLRAAVFGKVGAFSQ
                     VEMNRFGTASLITRNTNDVQQVQLVVFMGLTIILLAPITGIGATIMAIRQDVPLSGLL
                     LVILPIMFTFIALIFRQAIPLFRSMQTKVDRINQVMREALAGVRVIRAFVRVEHEEQR
                     FDVASRDLFDTALRVNRLFAVTFPIMMAIFNISSVAVMWFGADRVGSGAMPIGNLTAF
                     LTYLMQILMSVLMAVFMFILVPRAAVSADRINEILEIDPLIKDAPDPTPISLTGPGIR
                     VELRNVEFRYPGAEQPVLRDVSFTAEPGRTTAIVGSTGSGKTTLVNLIPRFYDVTGGQ
                     VLVDGIDVRDVSREDLWKMLGVIPQQAFLFSGTIASNLRYGDADATAAELNHALDIAQ
                     ARDFVGEMKDGLESVIDQGGTNVSGGQRQRLAIARALVKRAPLYVFDDSFSALDFRTD
                     ARLRATLRRELAGATVIIVAQRVGTILNADRIVVLDEGRVVGVGTHKELMESSETYRE
                     IVFSQLTPQEAQVA"
     gene            complement(5866..>7246)
                     /locus_tag="VIF08_05845"
     CDS             complement(5866..>7246)
                     /locus_tag="VIF08_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY8135545.1"
                     /translation="HTAYSNSTPLVALLGDVQRSHKGREAFQESDLVARFGGLAKWAA
                     QIVDPAAAAAQLGEGLSLMRSGRPGPVLLALPEDVLLEPVAARAKAKVAPVAPPAVEG
                     AAVAEVLALLGEAKRPVILAGGGVTAAGARDDLVALSERLRVPVACAFRRPTAFPNDH
                     PHYLGMTGYGAPSSVRARLENADALLVIGSRLNEIATFDYLIPRPRQKWAHVDLVPRA
                     RSVAGLRRANIALEADAAAFLRAALRQSRDYKPPRDRMKALAADRATYLAASTLPEAK
                     DWRGPGVDPAHVINVLQRVAPKDSIITSDAGNFALWLSRGFRYGSDQAFLGPTSGAMG
                     YGLPAAVAASLVAPNRPVIAVCGDGGLAMTMNELETALRSGATPVVLVFDNQRYGTIA
                     MHQRNEGYSDTATELGRIDFAAVARACGAQGVRVTSNTEFGPALRAALRTRRTSLLHI
                     EIDPRWVKP"
BASE COUNT         1150 a         2386 c         2519 g         1191 t
ORIGIN      
        1 tagagtgacc gcgccggacg tctgacgcgg cgagagaaga ggaagaggag gctcaggtgc
       61 tcaaggaatt ccgtgacttc ctattgcgcg ggaatgtcat cgatcttgcc gtggcggtgg
      121 tcatcggcgg tgcattcggc gccgtcgtca cctcctttgt cggtgacgtt ctgaccccgc
      181 tcctggggct cattggcgtg cccgacttct cgacgctcgc gatcaccacg ccgggcggcg
      241 cagtcattgc ctatggcctg ttcctgaatg cgctgatctc cttcgtgctg atcgcagccg
      301 caatcttctt cttcgtcatc aagccgctca actcgctcga gaagcgccgc caggccggcg
      361 cggagacctc gcctacgacc aagacgtgca ccgagtgcgc atcggccatc ccgctggcgg
      421 cccggcgctg cccgaactgc acccagccgc agccgtaatg ggacggcgcg gctggctacg
      481 cggggcgagc tatgcggcgc tcgtcgctgt cctggccgcg gcctgttcgg gtggtcccaa
      541 gagcccggtc ggtcccgtcg gtccgggcgg agcgacgctg ccgccgcagg cgatcggcaa
      601 cgccgtgttg ttcgatccgt ggcagataac ggacccgttc tcgggcggcg tggccgccgt
      661 gacctacctg gttccgacgg gctggcagtc gagcggccag gtcgtgtgga tgccggcgtg
      721 gcagaggctc gtagacctcc agaccatcgt gagcgatccc gtgtcgggcg ccacggtcga
      781 gtggctcccc atccaggatt tcattttcgg ccaggcgcca gccgggctcg agccacccat
      841 cgggggcaac taccagggca agatgtgggt gccgccggtg accgacccgg tgcagttcgt
      901 gtctgatttc tggatgtcgg gacccctggc gcacctggct gacgcaacac ttctcagcct
      961 tgaacaggtc ccggccgtgg ccgatgagtt ccttcttggt ttcggtggcc cagccaacgc
     1021 agcggcgtac cgaatgcgct attccttcac gcaaaacggc caagcatggg acgagcaggt
     1081 caatttcgct ttgctgtatg cgagtggcgg cggcatcgac tcctggtacg tgaacttcgc
     1141 ctacacgatg cgcgcgccac agggcgtgct cgatcaactc gcgccagtgc tgtccacgat
     1201 cgtgtcgtcg cgtgactcca cgccgcaatt ccaggccata gtcaccatga cgcagcgact
     1261 gttcacgcag ggcatccagc agcagatggc cgacacggag gcgttcggca ggctgctggc
     1321 cgagcagcgc gcccagaccg atgcgctgca ggcgcagatc accgcggacc ggcaggcttc
     1381 gcaggaccgc atcgcacagt tgcgcggcga ggtgttgtcc ggggtccaga cgttctctga
     1441 tccggtaaac ggtcgactgg tccagctgcc cgcgggccaa tcaacctact gggtcaacaa
     1501 ccagggccag tacttcgcct cggatcaacc gggcttcgat cccagcacgc ttggcggcgg
     1561 ctggacccag ctccagccgc gccagttcta gggatggtcg tagcgcgcat cacgccggat
     1621 gagtgggcgc tcctgcgcga catgcggctg cgcgcgctgc gcgacgcgcc ggaggcgttc
     1681 ggccagacct acgagaacgc ggccgccgaa ccggagctcg agtggcgcag tgccgcgcgc
     1741 gcggcctcgg cgggtgaccg ccgcgcctgg ttcttcgccc gttcggatgg gtccgcggca
     1801 gaggttaggt cagcggccgg cgcgccaccg gcggagctgg gcatggtcca ggcgcggcgg
     1861 cggccgcccg acgactgcct cgtcttctcg atgtgggtcg cgccggaggc gcgccgcggc
     1921 ggcgtgggac gcgagctcat tgcggccgcg gccgactggg cgcgatcgtg ggatggccgg
     1981 cgcattgtgc tgtgggtgac cggcgtcaat gagagcgcga tgcgtttcta cgagcgggtc
     2041 ggctttcgcc tgctttccga aggcccggac atggaatctg gtcgcgcctt tggcgcgtta
     2101 gcgatggagc tgccgctcta gcgtcgccta ggcaacggcg gcgagcgcct ctacgaactg
     2161 gctctggtag agctcgttgt agaagccttt ggcctccagc agctgctggt gcgtgcccgt
     2221 ctcgacgatc ctgccgtggt ccatcacgac gatcaggtca gcgtcgcgga tggtcgacag
     2281 ccgatgggcg atgacgaacg tggtccggtc cgacatcagc ttggccatgg cgcgctggat
     2341 cagcacctcg gtccgggtgt cgaccgatga ggtggcctcg tccaggatca ggatcctcgg
     2401 gtcggccaga aaggcacgag cgatcgtgag gagctgcttt tcgccctgcg acaggttggt
     2461 ggcgtcgtcg tcgacgaccg tttcgtagcc acccggcagc gtccgcacga agtggtcgac
     2521 gtgggccttc tctgccgcgg cgacgatctc cgcgtcggtc gcgccctcgc ggccgtaggc
     2581 gatgttgtcg cggatcgtgc cgttgaacag ccaggcgtcc tgcaggacca tgccgaactc
     2641 gcgccgcacg tcctcgcggc gcacgctgcg agtatcgatg ccgtcgatcg tgatgcgccc
     2701 cgagtcgatc tcgtagaagc gcatcagcag gttgacgagc gtcgtcttgc cggcgccggt
     2761 cgggccgacg atggccacgg tcgcgcctgg cttcgcggtg agtgacaggt gctcgatgag
     2821 cggcgtatcg ggcaggtagc ggaacgacac gtcctcgaag gcgaccttgc ccgcgacctc
     2881 gcccagcgcg ggggcgtcgg ccggatcggt caccatctcc gcctcatcga gcagctcgaa
     2941 cacgcgctcg gctgacgcaa ccgccgactg gagcacgttg gcaatgctcg ccgcctggag
     3001 gatgggctgg ctgaattggc gcgagtactg gatgaaggcg accacgtcgc ccagggtcat
     3061 ctgtccggtg gcgacgcgca ggccgccgat gacggcgatg ccgacgtagt tgaagttgcc
     3121 gatcacgttg agcgccggct ggatgatgcc gctgatgaac tgggcgcggt acgacgcctg
     3181 gtagagccgc tcgttctccg tggcgaaccg cgcagcagcc tcgcgttggc ggccgaacac
     3241 ggtgatgatg ttgtgcccgg tgtgcgcctc ctccacctgg ccgttcaggg cgccggtcga
     3301 cgcccactgg gcgacgaact gcttctgtga gcggcggatg atcagcaggc tcacgaccag
     3361 cgagagcggt acggcgagca gtgagatcag cgccagctcc cagctgatcg tcaccatcat
     3421 gatcaggacg ccgatgaccg tcagtaccga cgtcatgagc tgggtcaggc tctgctgcag
     3481 cgactggccg atgttgtcga tgtcgttggt gacgcggctg agcacgtcac cgcgcgggtg
     3541 atcgtcgaag tacttgagcg gtaaccgacc gagcttcacg tcgacggcgg cgcgcagccg
     3601 gtagaccgtg cgctgcgtga cgccggccat gatgtacgcc tggccccagg cgaagatcga
     3661 ggcgacaagg tagatgccga ccagcaccgc caggatgttg gccaggcggg cgaagtcgat
     3721 gccggcgccc gggaccaggg tcatgccgtg gagcatgtcg gcgaactgac cctggccgcg
     3781 cgatcgcagc agctcctcga cctgtgcctg ggtcatgccg gcaggtagct cctggccgat
     3841 tgccccggca aagatgatgt tcgttgcgtc gcccaggatc tttggcccga cgacctggag
     3901 cacgacactg atcgcgcaca gcgcgataac ggccacgatc agccagcgct cgggcgccag
     3961 ctggcgcatc aggcgtcgga aggacaggcc gaagttcttc gacttctccg cgggtgggcc
     4021 gcccatcatc atcgccatgg gccccccgcg cggcccgcgc ggacctgcag gcgggccgcc
     4081 tttgccctgc tgtggccggc cgggcatctg gccgctcatg cgacctgcgc ctcctgcggg
     4141 gtgagctggc tgaacacgat ctcgcggtag gtctcggacg actccatgag ctccttgtgg
     4201 gtgcccacac cgaccactcg tccctcgtcg agcacgacga tgcggtcggc gttcaggatc
     4261 gtgccgacac gctgggcgac gatgatcacg gtcgcgcctg ccagctcgcg gcgcagcgtc
     4321 gcgcgcagcc gcgcgtcggt gcggaagtcg agcgcgctga agctgtcgtc gaacacgtac
     4381 agcggcgccc gcttgaccag cgcgcgggcg atcgcgaggc gctggcgctg gccgcccgac
     4441 acgttcgtgc cgccctggtc gatcaccgac tccagcccgt ccttcatctc gcccacgaag
     4501 tcgcgcgcct gggcgatatc gagcgcgtga ttgagctccg cagcagtggc atcggcatcg
     4561 ccgtagcgca ggttgctggc gatcgtgccg ctgaagagga aggcctgttg cgggatgacg
     4621 cccagcatct tccacaggtc ctcgcggctc acatcacgga cgtcgatccc atcgacgagc
     4681 acctggccgc cggtgacgtc gtagaagcgc ggtatcaggt tgacgagcgt cgtcttgccc
     4741 gacccggtgc tgcccacgat ggccgtggtc cggcccggtt cggccgtgaa cgaaacatcg
     4801 cgcagcacgg gctgctccgc gccgggatag cggaactcga cgttccgcag ctccaccctg
     4861 atgcccgggc cggtcagcga gatgggtgtc gggtccggcg cgtccttgat gagcgggtcg
     4921 atctcgagga tctcgttgat gcggtcggct gagaccgccg cgcgcggcac gaggatgaac
     4981 atgaagaccg ccatcaggac gctcatcagg atctgcatga gataggtcag gaatgcggtc
     5041 aggttgccaa tgggcatcgc gccactgccc acccggtccg cgccgaacca catcacggcg
     5101 accgagctga tgttgaagat cgccatcatg atcgggaagg tgaccgcgaa gagacggttg
     5161 acgcgcaggg ccgtgtcgaa caggtcgcgg ctggcgacgt cgaagcgctg ctcctcgtgc
     5221 tccacgcgca cgaacgcgcg gatcacgcgc acgcccgcca gtgcctcgcg catgacctgg
     5281 ttgatccggt cgaccttggt ctgcatggag cggaagagtg ggatcgcctg acggaagatc
     5341 agggcgatga acgtgaacat gatgggcagg atcaccagca gcaggcccga tagcggcacg
     5401 tcctggcgga tggccatgat ggtggcgccg atgcccgtga tgggcgcgag caggatgatc
     5461 gtgagaccca tgaatacgac gagctgcacc tgctggacat cattggtgtt gcgcgtgatc
     5521 agagacgccg tgccgaagcg attcatctcg acctggctga acgcgcccac cttgccgaac
     5581 accgccgcgc gcaggtcgcg tccgaagccc atcgcgacgc gggccgagta gtagaccgcg
     5641 acgagcgagg tgatggccag gatgatcgtg acgccgacca tgaccagccc cacgttcagg
     5701 atgaggttgc tatccccggt ggccacgccc tggttgatga tctcggcgtt caggtcaggc
     5761 agatacagct gggtcagcgc ctggacgagc agcagtacga gcacgatggc gatctgcccc
     5821 ttgtaggcgc ccaggtagcg gcgcaggagg cggatcacct cagcctcaag gcttgaccca
     5881 gcgcggatcg atctcgatgt gcaggagcga agttcggcgc gtccgcagcg ccgcgcgcag
     5941 ggcaggcccg aactcggtgt tgctcgttac ccggaccccc tgggcgccgc acgcgcgggc
     6001 aaccgccgca aagtcgatgc gcccgagctc agtggccgta tcggaatagc cctcattgcg
     6061 ctgatgcatg gcgatcgtgc cgtagcgctg gttgtcgaag acgaggacca ccggcgtcgc
     6121 gccgctgcgc agcgcagtct ccagctcgtt catcgtcatc gccaaacctc cgtcgccaca
     6181 gacggcgatc accggccgat tcggcgcgac gaggctggcc gcaaccgctg ccggcagacc
     6241 gtagcccatc gcgcccgaag tcgggccgag gaaggcctgg tcgctgccgt agcggaagcc
     6301 gcgcgacagc cacagcgcga agttgccggc atcgctggta atgatcgagt cctttggcgc
     6361 gacgcgttgc agcacgttga tgacatgcgc cggatcgaca cccgggccgc gccagtcctt
     6421 cgcctcgggg agtgtggagg cggcaaggta ggtcgcgcga tctgcggcga gcgccttcat
     6481 gcggtctctc ggcggtttgt agtcgcgcga ctgccgcagc gcagcgcgca gaaaggcagc
     6541 tgcgtcggct tcgagggcga tgttggcgcg acgcaggcca gcgaccgacc tggcgcgcgg
     6601 cacaagatcc acgtgggccc acttctgacg cggccgcggg atcagatagt cgaaggtggc
     6661 gatctcgttg agacgcgagc cgatgacgag cagcgcgtct gcgttctcga ggcgtgccct
     6721 gacgctggac ggcgcaccgt agcccgtcat gcccaggtag tgcggatggt cgttggggaa
     6781 cgccgtcggc cgtcggaacg cgcaggcgac ggggacccgg agccgctccg acagtgccac
     6841 gaggtcgtcg cgcgcgccgg ccgcggtcac gccgccgccg gccaggatga ccgggcgctt
     6901 ggcctcgccg agcagcgcca gcacctctgc caccgccgca ccctcgacgg ctggcggcgc
     6961 gaccggcgcg accttcgcct ttgcccgcgc cgccacgggt tcgagcagca cgtcctcggg
     7021 cagggccaac agcacggggc ctgggcggcc cgagcgcatc agcgacagcc cctcccccag
     7081 ctgtgccgcg gcagcggccg gatcgacgat ctgcgctgcc cacttggcga gcccgccgaa
     7141 gcgcgcgacg agatcgctct cctggaaggc ctcgcggccc ttgtgcgagc gctggacgtc
     7201 gcccagcaaa gcgaccaacg gcgtcgagtt gctgtacgcg gtgtgg
//
LOCUS       DATBRX010000151         7143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3964135, whole genome shotgun sequence.
ACCESSION   DATBRX010000151 DATBRX010000000
VERSION     DATBRX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3964135"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="VIF08_05850"
     CDS             complement(<1..1320)
                     /locus_tag="VIF08_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8135546.1"
                     /translation="MRTTAAVGLRVDAHTGNELDAILRPRSIAVIGASRKRHSIGGEL
                     FHNLVDGDFAGAVYPVNANGGSVQGVHAYRSVLDIPDAIDLAIVAIPAEYVADAAREC
                     AAKGARGLIVLSAGFSETGDRGRQLQDELVATCRGSRIRLVGPNCMGVINTADDVRMN
                     GQFAPIAPLPGRLGVLAQSGALGLALIHEAGRLGLGLSSFVSVGNQADIATDDLLTYW
                     QDDPQTDVILLYLESIADPRRFARIARRVARTKPVIVVKSGRTGAGARATSSHTGALL
                     GASDITVDALFGQAGVIRTDTLAEMFDAAAVLASQPIPPSNRVAVLTNGGGPGILAAD
                     AADAAGLELPVLGEETRHRLSALLPGAAAVANPVDMIASATPAEYAQAIEIIGADPAI
                     DSLIVISGPPLATPASVLAGAVHDAVAHLPRRLSVATVFMSTEQPRAA"
     gene            complement(1324..1680)
                     /locus_tag="VIF08_05855"
     CDS             complement(1324..1680)
                     /locus_tag="VIF08_05855"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HEY8135547.1"
                     /translation="MKRTVASAAITREQRTIDCMLRIYCRGKHGTATEICPDCAALHS
                     YAMERLERCVYGTDKPTCRRCPVHCYRPAMREAIRGVMAYAGPRMLWSHPLLAIQHLL
                     KDRKPARERPAKAPTR"
     gene            complement(1744..1820)
                     /locus_tag="VIF08_05860"
     tRNA            complement(1744..1820)
                     /locus_tag="VIF08_05860"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1784..1786),aa:Val,seq:gac)
     gene            1862..2521
                     /locus_tag="VIF08_05865"
     CDS             1862..2521
                     /locus_tag="VIF08_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135548.1"
                     /translation="MGFVFAHGGLGLLPDHEAIMFLMPALPALFVATVVVGAALDRRG
                     ALGVPGRTVAVHTPLAIIAAALSLAAAGIHFAVIGEHLEEYVLFGVLFFALGWFQLVW
                     AQVYLVWPRPPVAATAIAVNLGAVLVWVMSRTVGLPIGPEPWLPEQVGFADILATSFE
                     IGIIGLLVPTLFRERFAGALAAQMPIQKAFVLSAFMVIAIGLLTAMALLPPAFEFLAF
                     S"
     gene            2546..3148
                     /locus_tag="VIF08_05870"
     CDS             2546..3148
                     /locus_tag="VIF08_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135549.1"
                     /translation="MLVDNDRSMLLMAILPIMLIGTILVATVLDRRGYLGPSFRLVGV
                     VFPLAAIAASLALAAAAILFAHAADAFARGRLLGLYTIGAGMFHLVWAQLYFASRASW
                     AAILGAAGTAVAMIGLVVFSVAGLSVLGESGVYLAGFQLALIVLLVPSIAPRLAATLA
                     QREMPVQRAVVLGAFGVATVALFTCYAVVGGALPVEAAVR"
     gene            complement(3240..3656)
                     /locus_tag="VIF08_05875"
     CDS             complement(3240..3656)
                     /locus_tag="VIF08_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135550.1"
                     /translation="MNYREMLFTPKGFLMVGGVVLLLLGLVGFFALKDPSMSFFWLDN
                     SENYAHLALGIVALAAVYVPGLNTALAPYYRWIVILVGLIALFFGVYGFIASGPAPNT
                     FGLTNLELGDNLLHLIVAAWAFAAAFWAPQRAMSSR"
     gene            complement(3792..4310)
                     /locus_tag="VIF08_05880"
     CDS             complement(3792..4310)
                     /locus_tag="VIF08_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135551.1"
                     /translation="MNRLRRAFLLTGLAMTLTACGGGGPQASGDAACPPVQRSGPDGQ
                     PVDLSGAWSGNDGGLYYIKQIGTCVWWSGLSNFEDQYPGQEWIMTFRGSMNDDGVING
                     DFVDVKSSNPGSGTMTIEARIDDVNGQGVIQLYRTASTGHEIGVTFWQPAPEPTPTEV
                     PAATNPPTTAPS"
     gene            complement(4322..5980)
                     /locus_tag="VIF08_05885"
     CDS             complement(4322..5980)
                     /locus_tag="VIF08_05885"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY8135552.1"
                     /translation="MSQHADLLISGRVATLAGDHGFGWQPGIAIAGGRVLAVGGESDL
                     EPLVGPRTERWRLAADELVMPGITDAHLHLMTLTLAADHIDLTGLDLPSALEAVARRH
                     RAMLDAGEADAWLFGHGWSAHDLGGWPTADMLERVAPGRRIELVAHDHHASWLSVAAL
                     RVAAISVNTPDPEGGVVRRDQSGEPTGILHETAGGLIESAIPEPPDSWVEKSLARVAQ
                     ELSVLGITGCHDPGELSADVQITRGPVFYRRLAGEGRLPLRVHASVRAPQLSTAIEAG
                     LRSGQSVGRYTMGWLKLFADGSLGSRSAFMLAPYSDAASNPPTGGPVGMILTPPEDLA
                     DLAHRAEANGIAVQIHAIGDAAVRAVLDVFEALPPAPHGLPLMRRMEHAQLVDPTDQP
                     RFGRLRVAASVQPVHLRSDIPQIRLGWGERGEYAIPLRALLDGDALIPFGTDAPVEQP
                     DPWPGIATAVVRRDPADDSAVPVGARQAIDLARAMRGACLDPALVAGLTDCGHLRPGA
                     KADLLVVPAAGFGDPLDAAALASTRPLATLIDGQVVHRGARFDP"
     gene            complement(5977..6957)
                     /locus_tag="VIF08_05890"
     CDS             complement(5977..6957)
                     /locus_tag="VIF08_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8135553.1"
                     /translation="MSRVAMVFTGGTISTLPDAAAGGNTPVLRGADILARSPRVARVA
                     EIEPIDWGLVPASHLRFAQMIDIARLVDEALARPDVAGAVVVQGTDTIEETALAYELL
                     VRSDKPVIVTGAMRDGSSPEFDGPQNLADAAVCAQSADLIGAGVQVVLNGLVVAARDV
                     VKSHTSAMDTFRPRDGAATGRVTAGRLVLDGTPARRVRLPRVPDHAVEDVHLVTAVAG
                     IEGTLLRALRPLKPAAVVVAATGTGNTGADLLVAATELMADGCVVVETTRAPRGTIQP
                     WYAFPGGGAQWQRAGAVPSAYDGPKSRVIVALALAAGLDRDALAALLWPR"
     gene            complement(6954..>7143)
                     /locus_tag="VIF08_05895"
     CDS             complement(6954..>7143)
                     /locus_tag="VIF08_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135554.1"
                     /translation="GSILRAHPTHDAPASEGVLIDAVDPVSLSGRSLTSPRRLTYEDG
                     EDGDNGETGDDLGGIETR"
BASE COUNT         1058 a         2407 c         2541 g         1137 t
ORIGIN      
        1 ggcggcacgc ggctgctcgg tcgacatgaa gaccgtcgcg acgctcaagc ggcgcggcag
       61 gtgcgcgacc gcgtcgtgga cggcgcccgc caacacgctc gccggggtgg ccagcggtgg
      121 gccggagatg acgatcagcg agtcgatcgc cggatccgcc ccgatgatct cgatcgcctg
      181 agcgtactcg gccggggtgg ctgaggcgat catgtcgacc gggttggcca ccgcggcagc
      241 gcccggcagc agggccgaca gtcggtggcg agtctcctcg cccagcacgg gcagctcgag
      301 accagccgca tcagcggcgt ccgcggcgag gatgcccggc ccgccgccgt tggtcagcac
      361 cgcgacccgg ttgctgggcg gtatgggctg gctggccaga acggcggcag cgtcgaacat
      421 ctcggccagg gtgtcggtcc tgatcacgcc cgcctggccg aacagtgcat cgacggtgat
      481 gtctgaagcg cccagcagcg cacccgtatg cgacgaggtg gcgcgggcgc cggcacccgt
      541 gcgcccgctc ttgacgacga tgaccggctt ggtgcgcgcg acccggcgcg cgatgcgcgc
      601 gaagcggcgc gggtcggcga tcgattcgag atacagcagg atgacgtccg tctgcgggtc
      661 gtcctgccag tacgtgagca ggtcgtcggt cgcgatgtcg gcctgattcc cgaccgatac
      721 gaaggacgac aggcccagtc ccagccggcc tgcctcgtgg atcagcgcga gacccagtgc
      781 gccactctgg gcgagcacgc ccagccgacc ggggagcggc gcgatcggcg cgaactggcc
      841 attcatgcgg acgtcgtccg cggtgttgat gacgcccatg cagttgggcc cgacaaggcg
      901 aatgcgagag ccgcggcagg tcgcaacgag ctcgtcctgg agctgccttc cgcgatcgcc
      961 cgtctcgctg aagcccgcgc tcagcacgat caggccgcgc gctcccttgg cggcgcactc
     1021 acgcgctgcg tcggccacgt actcggcggg gatggcgacg atcgcgagat cgatcgcgtc
     1081 cgggatgtcg aggacggagc ggtacgcgtg cacgccctgg acgctgccgc cgttcgcgtt
     1141 gaccgggtag acggcaccgg cgaagtcccc gtcgaccagg ttgtggaaca gctccccgcc
     1201 gatcgagtgg cgcttacggg acgcgccgat gaccgcgatc gaccgcggcc gcaggatcgc
     1261 gtccagttcg ttgccggtat gagcgtcgac acggagcccg accgccgccg tcgtgcgcat
     1321 cgactacctc gtcggagcct tggcgggtcg ttcccgcgcg ggcttgcgat ccttgagcag
     1381 gtgctggatg gcgaggaggg gatgcgacca cagcatgcgc ggccccgcgt acgccatgac
     1441 gccgcgaatg gcttcgcgca tagccggcct gtagcagtgc acgggacagc gccggcacgt
     1501 gggcttgtcg gtaccgtaga cgcagcgctc gaggcgttcc atggcgtagc tgtggagcgc
     1561 ggcacagtcg ggacagatct ctgtggcagt tccgtgcttg ccacggcagt agatccggag
     1621 catgcagtcg atcgtccgct gttcgcgcgt gatggctgcg ctggcgactg ttcgcttcac
     1681 gcgcgccact ctgcattgtg ctgccgcggc tcagcagtgc cgtttggcgt gaaaagtggc
     1741 gggtggtggg cgacactgga ctcgaaccag tgacctcttg catgtcaagc aagtgctcta
     1801 accagctgag ctagccgccc ggggtcgtcc attctagtcg gggccgcgta ggatgaccgc
     1861 gatggggttc gtcttcgcgc acggcggcct gggcttactg cccgaccacg aggcgatcat
     1921 gttcctgatg ccggcgctgc cggcgctgtt cgtcgcgacg gtcgtggttg gcgcggcact
     1981 cgacaggcgc ggcgcgctgg gcgtgcctgg gcgcacggtc gcggttcaca ctcccctcgc
     2041 gatcatcgcg gcggcgctga gtctcgcggc cgcgggcatt cactttgccg tgatcggcga
     2101 gcacctcgag gaatacgtcc tgttcggcgt tctgttcttc gcgctgggct ggttccagct
     2161 cgtctgggcg caggtctatc tcgtgtggcc acggccgccg gtcgcggcaa cggcgatcgc
     2221 ggtcaacctg ggcgccgtcc tggtctgggt gatgtcgcgc acggtcggat tgccgatcgg
     2281 acccgagccg tggctgcccg agcaggttgg cttcgcggac atcctcgcga cctcgtttga
     2341 gatcgggatc atcgggctgc tggtgccgac gctctttcgc gagcggttcg cgggcgcgct
     2401 tgccgctcag atgccgatcc agaaggcatt cgtgctgtca gcgttcatgg tcatcgccat
     2461 cggcctgctg actgctatgg cgctgttgcc gcccgcgttc gaattcctcg cgttttcgta
     2521 aggcgcgccg ctccagaatc gcggcatgtt ggtcgataac gaccgatcga tgctgctgat
     2581 ggcaatcctg ccgatcatgc tcatcggcac gatcctcgtg gccactgtcc tcgaccggcg
     2641 cggctacctg ggcccgtcgt tccgcctggt gggcgtggtc tttccgctcg cagcgatcgc
     2701 ggccagtctg gcgctcgccg cggccgcgat cttattcgcc cacgctgcgg acgcattcgc
     2761 gcgcggccgg ctgctcggcc tgtacacgat cggcgcgggc atgttccacc tggtatgggc
     2821 gcagctgtat ttcgcgtcgc gcgcgtcgtg ggcggcgatc cttggcgcgg ccggcacggc
     2881 ggtcgcgatg attggcctgg tggtcttcag cgtggccggg ctgtcggtcc tgggcgagtc
     2941 gggcgtctac ctcgccggct tccagctcgc cctgatcgtg ctgctggtgc catcgatcgc
     3001 gccgcggctg gcggcgaccc tggcccagcg cgagatgccg gtacagcgcg cggtcgtcct
     3061 tggcgccttc ggcgtggcga ccgtggcgct cttcacgtgc tatgccgtag tgggcggggc
     3121 gctgcccgtg gaggcggcgg tccgctagct ttggcccgcc tgatcgctcc gttccgggca
     3181 aagaaagacc cggctcgcct tgcgacgagc cgggccttct ggcttacgcc ggtaattccc
     3241 tagcgggagg acatggcgcg ctgcggcgcc cagaacgcgg ccgcgaacgc ccacgcggcg
     3301 acgatgagat ggagcaggtt gtcgccgagc tccaggttgg tcagcccgaa cgtgttcggt
     3361 gccgggcctg aggcgatgaa gccgtacacg ccgaagaaca gcgcgatcag gccgacgagg
     3421 atgacgatcc agcggtagta cggggccaga gccgtgttga ggccgggcac gtagaccgcc
     3481 gcgagggcga cgatgcccag cgcgaggtga gcgtagttct cgctgttgtc gagccagaag
     3541 aagctcatcg acggatcctt cagggcgaag aagccgacga ggccgagcag cagcagaacg
     3601 acgccgccaa ccatcaggaa gccctttggc gtaaagagca tctccctgta gttcatagag
     3661 atcgaattcc tccactcccg gtgggcgccg aagcggtcgt ccccccagtg accgagcgcc
     3721 actggcgcga atattgcgcc cacccggccc catgtcaacg ggcgtttatg tgatttgggg
     3781 tggggtcacg ctcaggaagg ggcagttgtc ggaggattgg tggctgcggg cacctcggtg
     3841 ggcgtcggct caggagcggg ttgccagaaa gtcacgccga tctcatgacc ggtcgacgcc
     3901 gtgcggtaga gctggatgac gccctggcca ttcacgtcgt cgatgcgcgc ctcgatcgtc
     3961 atcgtgccgc tgcccggatt gctgctcttc acgtcgacga aatcgccgtt gatcacaccg
     4021 tcatcgttca tagagccgcg gaaggtcatg atccactcct ggcccggata ctgatcctcg
     4081 aaattggaga ggcccgacca ccagacgcag gtcccgatct gcttgatgta gtacaggcca
     4141 ccgtcgttgc cgctccacgc tcccgacagg tccaccggct ggccgtcggg accgctgcgc
     4201 tggaccggcg ggcaggccgc gtcgccactg gcctgcggac cgccgccgcc acaggcggtc
     4261 agggtcattg cgagcccggt cagtaggaac gcgcgacgca gtcgattcat cgcctgaaat
     4321 tctacgggtc gaaccgtgcg ccacggtgca cgacctggcc atcgatcagc gtcgccaggg
     4381 gccgcgtgct tgccagcgcc gcggcgtcaa gcgggtcacc gaaaccggcg gcaggcacga
     4441 ccagcaggtc cgcctttgcc cccggcctga gatggccgca gtcggtcagg ccggcgacaa
     4501 gtgccgggtc caggcacgcg ccgcgcatgg cgcgggcgag atcgatcgcc tggcgcgctc
     4561 ccaccggaac ggccgagtca tcggcgggat cgcgccgcac cacggcggtg gcgatgcccg
     4621 gccatggatc gggctgctcg accggcgcgt cggtgccaaa gggaatcaga gcgtcgccgt
     4681 cgagcagggc gcgcaacggg atcgcgtact cgccgcgctc gccccagccg agacgaatct
     4741 ggggaatgtc gctgcgcagg tgaaccggct ggaccgacgc ggccacgcgc agccggccga
     4801 agcgcggctg gtcggtcggg tcgaccagct gggcatgctc catgcgccgc atcagcggca
     4861 ggccgtgcgg agcgggtggc agcgcctcga agacgtcgag cacggcccgc accgccgcgt
     4921 cgccaatggc gtgaatctgc acggcaatgc cgttcgcctc ggctcggtgc gcgagatcgg
     4981 ccagatcctc gggcggcgtc aggatcatgc cgactggtcc gccggtcggc ggattgctcg
     5041 cggcatcgct gtacggggcg agcatgaatg cgctgcgtga gcccagcgag ccatcggcga
     5101 agagcttgag ccagcccatc gtgtagcgac cgacgctctg gccgctgcgc aggccggcct
     5161 cgatcgcggt cgaaagctgt ggcgcgcgga ccgaggcgtg gacccgcagc ggcaggcggc
     5221 cctcccctgc aaggcggcga tagaagaccg ggccgcgggt gatctggaca tcggcgctga
     5281 gctcgcccgg atcgtggcaa ccggtgatgc ccagcacgga cagctcctgg gcgacgcgcg
     5341 cgagcgactt ttcgacccat gaatcgggcg gctccggaat ggccgattcg atcagcccgc
     5401 cagctgtctc atgcaggatg cccgttggct cgccgctttg gtcgcgccgc acgacgccgc
     5461 cctcgggatc tggcgtgttg acgctgatcg ctgccacgcg aagagcggca acgctcagcc
     5521 agctagcgtg gtgatcgtgg gccacgagct cgatgcgccg gccgggcgcg acacgttcga
     5581 gcatgtcggc agtgggccag ccgccaaggt cgtgagccga ccagccgtgg ccgaacagcc
     5641 aggcgtcagc ctctccggcg tcgagcatgg cgcgatggcg ccgcgcgact gcttcgagcg
     5701 cggagggcag gtcgagcccg gtcaggtcta tgtggtcggc cgccagggtg agcgtcatga
     5761 gatgcaggtg ggcatcggtg atcccgggca tgacgagttc gtcagcggcc agccgccagc
     5821 gctccgtccg agggccgacc aacggctcca gatccgattc accgcccacg gccagcacgc
     5881 gaccacccgc gatggcgatc cccggctgcc agccaaatcc atggtcgccg gccaaggtgg
     5941 cgacccgtcc gctgatcaac aggtcggcgt gctggctcac ctgggccaca gcagcgccgc
     6001 cagtgcgtcc cggtccagcc ccgcggccag ggcgagcgcc acgatgacgc gcgacttggg
     6061 tccgtcgtac gcggacggca cggcgcccgc gcgctgccac tgcgcaccgc cacccgggaa
     6121 cgcgtaccag ggctggatcg tgccgcgcgg cgcccgggtg gtttcgacca cgacacagcc
     6181 gtcggccatg agctcggtcg cggccacaag aaggtctgca cccgtgttac ccgtgcccgt
     6241 cgcggcgacg accacggccg cgggtttcaa ggggcgaagg gcgcgcagca gcgtgccttc
     6301 aatgccggcg acggccgtga cgaggtgcac gtcttcgacc gcgtgatccg gcacgcgcgg
     6361 cagacggacg cgccgggccg gcgtgccgtc gaggacgagg cgcccggctg tgacccgccc
     6421 tgtggcggca ccgtcgcgcg ggcggaaggt gtccatcgcg ctcgtgtggc tcttcacgac
     6481 gtcgcgcgcg gccacgacca ggccattgag cacgacctgt accccggccc cgatcaggtc
     6541 cgccgactgg gcgcagacgg ccgcgtcggc caggttctgc ggcccgtcga actcgggcga
     6601 tgagccatca cgcatcgcac cggtgacgat cacgggcttg tccgatcgaa ccagcagctc
     6661 gtacgcgagt gccgtctcct cgatggtgtc cgtgccctgg accacaacgg ccccggcaac
     6721 atcaggtcgt gcgagtgcct catcaacgag gcgcgcgatg tcgatcatct gggcaaagcg
     6781 caggtgcgac gcgggcacga ggccccagtc gatgggctct atctcagcga cgcgcgcgac
     6841 ccgcggtgag cgcgcgagga tgtccgcacc gcgcagcacc ggcgtgttgc cgccggccgc
     6901 agcgtcaggc agcgtcgaga tggtcccgcc ggtgaagacc atcgcgacgc ggctcatctc
     6961 gtttcgatac cgcccaaatc atcgccggtt tcgccgttgt cgccgtcctc gccatcctcg
     7021 taggtcagtc tgcgtggaga cgtaagtgat cggccggaca ggctgaccgg gtccacggcg
     7081 tcgatcaaca ccccctccga tgcgggcgcg tcatgagtgg gatgcgcgcg caggatcgag
     7141 ccc
//
LOCUS       DATBRX010000152         4570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3406563, whole genome shotgun sequence.
ACCESSION   DATBRX010000152 DATBRX010000000
VERSION     DATBRX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3406563"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VIF08_05900"
     CDS             <1..348
                     /locus_tag="VIF08_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HEY8135555.1"
                     /translation="GVVLPLSESGVYRERFGTRHRAALGITEQTDAIAVVVSEETGSV
                     SLVERGRIVRDLDEPRLGKALVELLEHSPLTAVKVPERVRLPERASAIGRNIASRRPR
                     RRRDPAPTSNRTE"
     gene            345..1319
                     /locus_tag="VIF08_05905"
     CDS             345..1319
                     /locus_tag="VIF08_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HEY8135556.1"
                     /translation="MRRVTDFLRRNWPLRLGAILLATVLYTGLVLGQNVRTWNGEVPI
                     ERIRQASNVTLVSEIPPVTMIRYRAPIDVLLSPNLFAANVDLSGVVPLPGGPPVTVPV
                     TLVSLDQRIQIVDFQPREVQVQLDPVAERTLPVTVELGTIPDGVAVGSPQIEPSAVTL
                     RGAASRVESVNQVVARISIDASALNVDRDVDLVAVDGNGNPVPGVEIDPERVRVRVAV
                     ARELATRTLPVVPVLVGAPAPGFRIIAISVEPLIVTLSGEAATIGGLDGALTEAINVA
                     GRTTDLEAIVNLDLPEGVSVNGAEQVKVVLTIEPQPTPAPSISPSPSP"
     gene            1316..2647
                     /gene="glmM"
                     /locus_tag="VIF08_05910"
     CDS             1316..2647
                     /gene="glmM"
                     /locus_tag="VIF08_05910"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY8135557.1"
                     /translation="MTRLFGTDGIRGVANEDLTPKLAFDLGRATASHMLGNGASLLLG
                     QDTRRSGDMLASAVAAGAMSLGADVHRLGVCPTPALIHLTAARDHGAGIMVSASHNPA
                     RDNGLKVVDERGLKLDDPLEEELESLIFRADELAGPDNSRLGREIDATDALEDYVSDR
                     LEIAARCASSARIVLDCANGSACDVAPRILAGTGASVDVRFNEPDGMNINLDCGATAP
                     DALARIVADTKADIGFALDGDADRCVAVDEMGRVVDGDQLIGMIALDRLGRGALANGI
                     VVASVLSNGGLEKAVTAAGGSLARTPVGDKYILDGMLVMDAMLGGEKSGHIIVREHAV
                     AGDGIVTALEVLSILARTGKRLSELAAQITLFPQQQRAVSVRHKDQWEADPTFTRAVD
                     EARRSLKDGGRLLVRPSGTESALRIMVEGEDEKQVLALADALAALAAERLN"
     gene            2671..4500
                     /gene="glmS"
                     /locus_tag="VIF08_05915"
     CDS             2671..4500
                     /gene="glmS"
                     /locus_tag="VIF08_05915"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY8135558.1"
                     /translation="MCGIVGYIGPRQAAPILMEGLARLEYRGYDSAGIALVTSGGDMF
                     VEKRAGKLENLRTALAEDGTPTAAIGLAHTRWATHGRPNDLNAHPHTDCTGQITVIHN
                     GIIENFKQLRDDLKAGGHKLSSDTDTEALAHLIEDAYAGDIAEATRAALRQAHGAYAV
                     AVMHAAEPNRLVGARMNVPLIVGLGDGENFLASDVAAVLAHTRRVIFLDEGDVADINA
                     QSVTVTGVDGEPRERLVHTIDWDIEKAEKGGYPHFMLKEMHEQPEALRAAITGRVHDS
                     TIELEELEPIVARLRDIERIEFVACGTANYASAVGAYVMQDWLSIPARWNIGSEFRYS
                     PPPLDKRTLVIAVTQSGETADTIAPTRYARAQGCPIIAVTNTVGSAITREADAVLFLQ
                     AGPEIAVVATKTFATQVTTLVLLAAAMAKLRGRLPQERERQLIAELRALPDKAARALV
                     LAEPARELARRYVNSRGFMFVGRGVTYPVALEGALKLKEVSYVHAEGYAAGELKHGPI
                     SLLDAEVPLVAIATRSSVQEKLISNVMEGRARDARVLAVATEGDDAMTEYADDILWVP
                     ESIEELSPVLAAIPLQLFAYHTAVARGTDVDQPRNLAKSVTVE"
BASE COUNT          726 a         1588 c         1573 g          683 t
ORIGIN      
        1 ggcgtcgtcc tgccgttgag cgagagcggc gtctatcgcg agcgcttcgg cacgcgccac
       61 cgcgcggcgc tggggatcac cgagcagacg gacgcgatcg ctgtggtggt cagcgaggag
      121 acgggtagcg tcagcctcgt agagcgcggc cggatcgtgc gcgacctcga tgagccgcgc
      181 ctgggcaagg cgctggtcga gctgctcgag cattcgcccc tgaccgccgt caaggtgccg
      241 gaacgggtgc gactgcctga gcgcgccagc gcgatcggtc gcaacatcgc ctcgcgccgg
      301 ccgcgtcgcc gccgcgatcc ggcccccacg agcaacagaa cagagtgagg cgcgtcaccg
      361 acttcctgcg gcgcaactgg ccgctccgcc tgggagcgat cctgctcgcc accgtgctct
      421 atacgggctt ggtgctgggc cagaacgtgc gcacctggaa cggtgaggtg ccgatcgagc
      481 gcatccgcca agcatcgaac gtgacgctcg tcagcgagat cccgcccgtc acgatgatcc
      541 gttatcgcgc gccgatcgac gtcctgctgt cgcccaacct gttcgccgcc aacgttgacc
      601 tgtcgggcgt cgtcccattg cccggcggcc caccagtcac cgtgcccgtg accctcgtat
      661 cgctcgacca acgcatccag atcgtggact tccagccccg cgaagtgcag gtccagctgg
      721 atcccgtcgc tgagcggacg ctgcctgtca cggtcgagct gggcacgatc ccggatgggg
      781 tcgcggtggg ctcgccacag atcgagccca gcgcggtcac gctgcgtggc gccgcgtcgc
      841 gcgttgagtc ggtcaaccag gtcgtggctc gaatcagcat cgacgcatct gcactgaacg
      901 tcgaccgtga cgtcgacctg gtagccgtcg atggcaacgg caacccggtg ccgggcgtgg
      961 agatcgatcc ggagcgggtg cgcgtgcgtg tcgcggtcgc gcgcgagctc gccaccagga
     1021 ccctgcccgt ggtgcccgtg cttgtcggcg cgcccgcccc aggctttcgc atcatcgcga
     1081 tcagcgtcga gccgctgatc gtcacgctga gcggcgaagc tgccacgatc ggcgggctcg
     1141 acggcgcgct gaccgaggca atcaatgtcg cgggccggac aaccgacctg gaggcgatcg
     1201 tcaacctcga cctgcccgaa ggtgtcagcg tgaatggcgc ggagcaggtg aaggtggtgc
     1261 tgacgatcga gccgcagccc actcccgcac cctcgatctc gccgagtccc tctccgtgac
     1321 ccgcctcttc ggcactgatg gcatccgcgg cgttgccaac gaggacctga cgcccaagct
     1381 ggcgttcgac ctgggccgcg caactgcgtc gcacatgctg ggcaatggcg cttcgctgct
     1441 gctgggccag gacacgcgcc gctcgggcga catgctggcc tccgcggtcg cggctggggc
     1501 aatgtccctg ggcgcggatg tgcaccgact tggcgtctgc ccgacacccg ccctgattca
     1561 cctcaccgca gcgcgcgacc atggcgccgg catcatggtc agcgcgtcac acaacccggc
     1621 gcgcgataac gggctgaagg tggtcgacga gcgcggcctg aagctcgacg atccgctcga
     1681 ggaggagctc gagtcgctca tcttccgtgc cgacgagctg gccggcccgg acaactcccg
     1741 gctgggccgc gagatcgacg cgaccgacgc gctcgaggac tacgtcagcg accggctcga
     1801 gatcgccgcg cgctgcgcca gctccgcgag aatcgtgctc gactgcgcga acggctcggc
     1861 ctgcgacgtg gcgccgcgca tcctggccgg caccggcgcc agcgttgacg tgcgtttcaa
     1921 cgagcccgac ggcatgaaca tcaacctcga ctgcggcgcc accgcgcccg acgcgctggc
     1981 ccggatcgtg gccgacacga aggccgacat cggcttcgcg ctcgacggcg acgccgatcg
     2041 gtgcgtggcg gtggacgaga tggggcgggt cgtcgacggc gaccagctca tcggcatgat
     2101 cgccctcgat cggctaggtc ggggtgccct cgccaatggc atcgtggtcg ccagcgtcct
     2161 gtccaacggc ggcttggaga aggctgtaac ggccgccggc ggttcgctgg cgcggacgcc
     2221 ggtcggcgac aagtacatcc ttgatggcat gctggtcatg gacgcgatgc tcggcggtga
     2281 gaagagcggc cacatcatcg tgcgcgagca cgcggtggcc ggcgacggca tcgtcaccgc
     2341 gctcgaggtg ctgtcgatcc tcgcgcgcac cggcaagcgc ctgtcagagc tggccgcgca
     2401 gatcacgctc tttccgcagc agcaacgcgc tgtctcggtt cgtcacaagg accagtggga
     2461 ggccgatccc actttcaccc gcgcggtcga cgaggcgcgg cggtcgctca aggatggcgg
     2521 ccgactactc gtccggccgt cgggcaccga atcggcgctg cgaatcatgg tcgaaggcga
     2581 agacgagaag caggtcctgg cgctggccga cgcccttgct gcgctcgctg cggagcggct
     2641 gaactaggac gcggagggag cagatctcga atgtgtggca tcgtcggtta catcggcccg
     2701 cgccaggcgg cgcccatcct catggagggc ctcgcccggc ttgaatatcg cggctacgac
     2761 tcggccggca tcgccctggt cacgagcggc ggcgacatgt tcgtcgagaa gcgcgcgggc
     2821 aagctggaga acctgcgcac cgcgctcgcc gaggacggca ccccgactgc cgccatcggc
     2881 ctcgcccaca cccgctgggc gacgcacggc cggccgaacg atctgaatgc ccatccccat
     2941 accgactgca cgggccagat cacggtcatc cacaacggca tcatcgagaa cttcaagcag
     3001 ctgcgcgacg atctgaaggc gggcggccac aagctctcct ccgataccga cacggaggcc
     3061 ctggcgcacc tgatcgaaga cgcctacgcg ggcgatatcg cggaggccac ccgcgcggcg
     3121 ctgcgccagg ctcacggcgc gtacgccgtc gctgtgatgc atgccgcgga gcccaatcgg
     3181 ctggtcgggg cgcgcatgaa tgtgccgctc atcgtgggcc tgggcgacgg cgagaacttc
     3241 ctggcgtccg atgtggcagc cgtgctcgcc cacacacgca gggtgatctt cctcgacgag
     3301 ggcgacgtcg ccgatatcaa cgcccaatcc gtgaccgtga ccggcgtcga cggtgagccg
     3361 cgcgaacggc tcgtgcacac gatcgactgg gacatcgaga aggcggagaa aggtggctac
     3421 ccgcacttca tgctcaagga gatgcacgag cagcccgagg cgctccgtgc cgcgatcacc
     3481 ggccgcgtgc acgactcgac catcgagctc gaggagctcg aaccgatcgt ggccaggttg
     3541 cgcgacatcg agcgcatcga gttcgtggcc tgcggcaccg ccaattacgc gagtgcggtc
     3601 ggggcgtacg tgatgcagga ctggctgagc atcccggcac gctggaacat cggctccgag
     3661 ttccgctatt caccgccgcc gctcgacaag aggacgctcg tcatcgcggt gacgcagtcg
     3721 ggcgagacgg cggacacgat cgcgcctacg cgctacgcgc gcgcccaggg ctgtccgatc
     3781 atcgctgtga ccaacaccgt cggcagcgcg atcacgcgcg aggccgacgc ggtgctcttc
     3841 ctccaggccg ggcccgagat cgcggtcgtc gcgaccaaga ccttcgccac gcaggtgacg
     3901 acgctcgtcc tgctggcggc cgcgatggcc aagctgcgcg gccgcctgcc acaggagcgc
     3961 gagcgccagc tcattgccga gctccgcgcg ctgcccgaca aggccgctcg ggcactcgtt
     4021 ctggccgagc ccgcccggga gctcgcgcgg cgctacgtca actcgcgcgg cttcatgttc
     4081 gtgggccgcg gcgtgaccta cccggtcgcg ctcgaaggcg ccctcaagct caaggaagtc
     4141 tcgtacgtcc acgccgaggg ctatgctgcg ggcgagctca agcatggccc gatctctctg
     4201 ctcgatgccg aagtgccgtt ggttgccatc gcgaccaggt cgtcggtgca ggagaagctg
     4261 atctcgaacg tcatggaagg tcgggcgcgc gatgcgcggg tcctcgcggt ggcgaccgag
     4321 ggtgacgatg cgatgacgga atacgccgac gacatcctgt gggtgccgga gtccatcgag
     4381 gagctttcgc ccgtcctcgc ggcgatcccg ctccagctct tcgcgtacca caccgcggtt
     4441 gcgcggggca cggacgtcga ccagccgcgc aacctcgcca agtcggtgac ggtggagtag
     4501 ggcggccagc gccgctcagc gttcggccgg cggggcgtgc ggcctgagcg ggttgatcgg
     4561 ccgctagagt
//
LOCUS       DATBRX010000153         4627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1952767, whole genome shotgun sequence.
ACCESSION   DATBRX010000153 DATBRX010000000
VERSION     DATBRX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952767"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="VIF08_05920"
     CDS             complement(<1..1118)
                     /locus_tag="VIF08_05920"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8135559.1"
                     /translation="MGEPRHTNRLARETSPYLLQHARNPVDWYPWGSEALARARELDR
                     PIFLSIGYAACHWCHVMERESFEDPETARMLNEGFVAIKVDREERPDLDAIYMDAVQR
                     MTGGGGWPMSVFLTPDGRPFFGGTYFPDSPRHGLPSFRQVLAGVSEAWRERRDEVVRS
                     SSDLAHAITGHAGPPSDAALDPVALDDALAAIEQGFDARTGGWGSAPKFPQPMTIEFL
                     LRLAATGRAQERAVAIARRSLDAMAAGGIHDHLGGGFARYATDSVWLVPHFEKMLYDN
                     AQLARVYVHAWQLTRDARYREVALDTLTFMERDLLTADGGFAASLDADTAGIEGATYV
                     WTLAEVEAVLADAALAAEFCRAYGVTAEGNWEGHTILSR"
     gene            1212..2048
                     /locus_tag="VIF08_05925"
     CDS             1212..2048
                     /locus_tag="VIF08_05925"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8135560.1"
                     /translation="MDQNGDFVGQLTRLGLTSYEAKAYLTLIRRDSFTAAQVARQSGL
                     PRQRIYDVLGSMVQKGLAVARPGNVVKYAATPPDVAINQLLAVQREDLSRIEGAAKQM
                     VADLEPAFEAGQRETDPLEYIEVLRDKRAINERFAELQKAVKREILVFTKPPYATPPQ
                     ENVEGLKVITSHEARSLYEFSVFDDPAVARGVQRFVEAGEQARFVPTLPLKLVIIDET
                     IVMFGMEDPVAGSADLTIVTVEHQSLAKVLKTAFEAIWETGLTFEQAREAATKREQKQ
                     PA"
     gene            2220..4091
                     /locus_tag="VIF08_05930"
     CDS             2220..4091
                     /locus_tag="VIF08_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8135561.1"
                     /translation="MTNENPFSFRRRVLLALIGALVLLAGLFPSATLADVVASQRYIV
                     VFKGDYALDGSYALGKGYALVTEQATYALDSTYALGTEYALYALNRTYTLLDQYALSD
                     EYALMGNYALFGQYALNSGYALARDYALGAGYALAQATAGYALDSSYALLDSTAGDYL
                     RDGYALLDAYALDQNYALARDYALYALSMAGGTITSDLSRQIGVFIVDSRNAQFAQIM
                     KSYALVQYVGKDFGVDMFPSYAEAIGSAQLEVWDSERADQPAAGEDPLEPAQWDMELI
                     RSQAAHAYTTGRKVDVGVLDSGIDGNHNDFRDNGLPTGHSNVDCVRGADFTFEGPGIG
                     SPLACVDNNFHGTHVAGTIGARRNGIGVVGVAPNATLVPIKVCDGDGHCYVSDAVEGI
                     TYAGDLGLDVVNMSFFVDDDAFQESTEFKCQSNDTQRAYRMAVERALAYARDEGVSLV
                     AALGNSDIDLANDAARGGKDCRTVPAQSPGVIGTVALGPLSEKSWYSSYGSGWADVSA
                     PGGNEEAADANCLTEILSTLPGNSYGCIQGTSMASPHTVGVVALIIGKYGKPDGNGGF
                     KWAPDSVWSKLKATAIDVGKKGYDKCYGYGRIDALRAARNLTSYSRDMSQTSCSEYT"
     gene            4266..>4627
                     /locus_tag="VIF08_05935"
     CDS             4266..>4627
                     /locus_tag="VIF08_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEY8135562.1"
                     /translation="MSVTAATAGLVLAAGAGKRFGGQKLLAPLNGTPILQHVLNLAAV
                     AALDPVVVVLGTDAEALVTRLWWRDEVRLINPVPEEGLSSSVRMGLASLALSDAGRAV
                     VLLGDQPLLSAAQLDVILA"
BASE COUNT          781 a         1598 c         1500 g          748 t
ORIGIN      
        1 cgcgacagga tcgtgtgccc ctcccagttg ccttccgccg tgacgccgta agcgcggcag
       61 aactccgcgg ctagcgccgc gtcggccagg acggcctcca cctcggccag cgtccagacg
      121 taggtcgcgc cctcgattcc ggcagtgtcg gcatccaggc tcgccgcgaa tccgccgtcc
      181 gcggtgagca gatcacgctc catgaatgtc agcgtgtcca gcgcgacctc gcgatagcgc
      241 gcgtcgcgcg tcagctgcca ggcatgcacg tagacgcgcg cgagctgcgc gttgtcgtag
      301 agcatcttct cgaagtgcgg cacgagccag accgaatcgg tcgcgtagcg cgcgaacccg
      361 ccgcccaggt ggtcgtggat gccgccggcg gccatggcgt cgagtgatcg gcgcgcgatt
      421 gccaccgccc tttcctgggc ccgtccggtg gctgccaggc gcagcaggaa ctcgattgtc
      481 atcggctggg gaaacttggg cgcgctgccc cagccgccgg tacgcgcgtc gaagccctgc
      541 tcgatcgctg cgagcgcgtc gtccagcgcc accggatcaa gagcagcgtc gctgggcgga
      601 cccgcgtgac ccgtgatggc gtgcgccagg tcgctgctcg agcgcacgac ctcgtcgcgc
      661 cgctcgcgcc acgcctcaga cacccccgcg agcacctggc ggaacgacgg caggccgtgg
      721 cgcggcgaat ccgggaaata cgtcccgcca aagaatggcc gaccgtcggg cgtcagaaag
      781 acgctcatcg gccagccccc gccgccggtc atccgctgca ccgcgtccat gtaaatcgca
      841 tcgagatcgg gccgctcctc gcgatcgacc ttgatcgcca cgaacccctc gttcagcatc
      901 cgcgccgtct cgggatcctc gaacgactcg cgctccatga cgtggcacca gtggcatgcc
      961 gcgtagccaa tcgacaggaa gatcggccgg tccagctcgc gtgccctggc caatgcctcc
     1021 gacccccacg ggtaccaatc gaccgggttg cgggcgtgct ggagcaggta cgggctggtc
     1081 tcgcgcgcga gtcggttggt gtgccgcggt tctcccacgc gcggccctgg ctctcccacg
     1141 cgccgatcgt acaggtcggg tggtgtcact ttggccagtg acaccaccgc ctgtggcggt
     1201 atgatctgag cgtggatcag aacggcgact tcgtcggtca gctaacccgt cttggcctga
     1261 cgagctacga ggccaaggcg tacctgacgc tcattcggcg cgactctttc acggccgcgc
     1321 aggttgcgcg ccaatcgggc ctgccgcgcc agcgtatcta cgacgtcctg gggagcatgg
     1381 tccagaaggg cctcgcggtc gcgcgcccgg gcaacgtggt caagtacgcg gctacaccgc
     1441 cggacgtggc gatcaatcag ttgctggcgg tccagcgcga ggatctctca cgcatcgaag
     1501 gcgccgccaa gcagatggtt gctgacctcg agcccgcctt cgaagccggc cagcgcgaga
     1561 ccgatccgct cgagtacatc gaggtgctgc gcgacaagcg cgcgatcaac gagcgcttcg
     1621 ccgagctcca aaaggcggtc aagcgtgaga ttctcgtctt caccaagccg ccctacgcga
     1681 cgccgcccca ggagaacgtc gaggggctca aggtcatcac gtcccatgag gcgcgctcgc
     1741 tgtacgagtt ctcggtgttc gacgatccgg ccgtcgcccg gggcgtgcag cgcttcgtcg
     1801 aggccggcga gcaggctcgc tttgtgccca cgctgccgct gaagctcgtg atcatcgacg
     1861 agacgatcgt gatgttcggc atggaggacc cggtcgcggg cagcgccgat ctgaccatcg
     1921 tgacagtcga gcaccagtcc ctggcgaagg tcctcaagac cgcgttcgag gcgatttggg
     1981 agaccggcct gacctttgag caggcccgcg aggccgcaac caagcgcgag cagaaacagc
     2041 ccgcctgagc gggctaccag acggctcctc ccggattcct cagcgggcct cccacgctca
     2101 cggaagaccg ttgttacaca cttgcaaggt gacacttccg tcagtggtgc ttgcatccgg
     2161 ctgactgggt taccactatg cgtggtgtcg tgctccttca gataaaggaa cccgttcgca
     2221 tgacaaacga gaacccgttc agcttccgcc gccgtgtctt gctggcgctg attggcgccc
     2281 tcgtgctgct cgccggcctg ttccccagcg cgacactggc cgacgtggtt gccagtcagc
     2341 gctacatcgt ggtcttcaag ggtgactacg cgctcgacgg cagctacgcc ctgggcaagg
     2401 gatatgcgct cgtcaccgaa caagcgacct atgcgctcga cagcacttac gcgctcggca
     2461 ctgagtacgc cctttatgcc ctgaaccgga cctacacgct cctcgaccaa tacgcgctga
     2521 gcgacgagta cgccctcatg ggtaactacg cgctattcgg ccagtacgcg ctcaacagcg
     2581 ggtatgcgct ggcgcgcgat tacgctctcg gagctggcta cgcgctggca caagcgactg
     2641 ctggctacgc gctggactcg tcctacgctc tgctcgacag caccgccggc gactacctgc
     2701 gcgacggcta cgctctgctc gatgcctacg cgctcgacca gaactacgcg ctcgcgcgcg
     2761 actacgcgct gtacgccctc agcatggccg gcggcaccat cacctctgat ctgagccgcc
     2821 agatcggcgt cttcatcgtc gattccagga acgcgcagtt cgcccagatc atgaagagtt
     2881 atgccctcgt gcagtacgtc ggcaaggact tcggcgtcga catgttcccg agctacgccg
     2941 aggcgatcgg cagcgcccag ctcgaggtct gggacagtga acgggccgac cagccggccg
     3001 cgggcgagga cccgctcgag cccgcccagt gggacatgga gctcatccgc tcgcaggccg
     3061 cccatgccta cacgaccggc cgcaaggtgg acgtcggcgt gctcgatagc ggcattgacg
     3121 gcaaccacaa cgacttccgc gacaacggtc tgcccaccgg ccactcgaac gttgactgtg
     3181 tgcgtggcgc cgacttcacg ttcgagggcc caggcatcgg cagcccgctg gcctgcgtag
     3241 acaacaactt ccatggcaca cacgtcgccg gcaccatcgg cgcgcggcgc aacggcatcg
     3301 gcgtcgtcgg tgtcgcgccc aacgcgacgc tcgtcccgat caaggtgtgc gacggcgatg
     3361 gtcactgcta cgtcagcgac gcagtcgaag gcatcaccta cgccggagat ctcggcctcg
     3421 atgtcgtgaa catgagcttc ttcgtcgacg acgacgcctt ccaggagtcg accgagttca
     3481 agtgccagag caacgacacc cagcgggcct accgcatggc ggtcgagcgc gcgctggcct
     3541 acgcccgcga cgaaggcgtc agcctggtcg ccgcgctggg caactcggac atcgaccttg
     3601 ccaacgacgc ggcgcgcggc ggcaaggact gccggacggt tcccgcccaa tcgccgggtg
     3661 tcatcggcac cgtggccctg ggtccgctga gcgagaagtc gtggtactcg agttacggct
     3721 cgggttgggc ggacgtgtcg gccccgggtg gtaacgaaga ggcggccgac gccaactgcc
     3781 tgaccgagat cctctcgacg ctcccgggca acagctacgg ctgcatccag ggcacctcga
     3841 tggcctcgcc ccacacggtc ggcgtcgtgg cgctgatcat cggcaagtac ggcaaaccag
     3901 acggcaacgg cggcttcaag tgggcaccgg atagcgtctg gtcgaagctc aaggcaaccg
     3961 cgatcgacgt cggcaagaag ggctacgaca agtgctacgg ctacggccgg atcgacgccc
     4021 tgcgcgccgc ccggaacctg acgagctaca gccgcgacat gagccagacg agctgctcgg
     4081 agtacacgtg aaatcccctg aacgctgacc gcgtcgatgc cccgtcagcc ctcgatgtaa
     4141 atggggggag tggtcagcgc agttaaatac gcgaccggta aggacgggtt ccggccgcgg
     4201 agcaagcggc ccccgggtct cagcgactcg ggggtcgctt catttctgcc ggtacgatca
     4261 ggcacgtgtc agtcacagcg gctacagccg ggctggtcct cgccgcgggc gcgggcaagc
     4321 gcttcggcgg ccagaagctg ctggcgccac tgaacggcac accgatcctc cagcatgtcc
     4381 tcaatctcgc cgcggttgcc gcgctcgacc cggtcgtcgt ggtccttggg accgacgcgg
     4441 aggcgctcgt gacgcggctg tggtggcgcg acgaggtgcg gctgatcaac ccggtgcccg
     4501 aggagggact ctcgtcatcg gtgcggatgg gcctggcgtc gctcgcgctg tcagacgcgg
     4561 ggcgtgcggt cgtcctgctg ggtgatcagc cactcctgtc tgccgcgcag ctcgatgtga
     4621 tcctcgc
//
LOCUS       DATBRX010000154         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1845119, whole genome shotgun sequence.
ACCESSION   DATBRX010000154 DATBRX010000000
VERSION     DATBRX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1845119"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VIF08_05940"
     CDS             <1..1467
                     /locus_tag="VIF08_05940"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8135563.1"
                     /translation="LGTVIVVILAGCTTSPVVPTASPIPTVAPMGTPSSLPSNPASAT
                     PTAAASATASIVPSVAPTGSPTGLQVNGSLITGHLDALQAIADANGGIRAAGTPGYDA
                     SADYVAQVMTDLGYQLERHPFDFPFFNETAPASVGFADVAWTGSEWVHAALYSASGDV
                     EAQLELVGDGLGTFGCDASEWSGFTSGHIAGVYGGGCFMRQKVELAQDAGAVAMISMY
                     PTWEQNEIKRPTLLDPDGISIPVVVAGREPALALANQEGNMASVFVQGDNHIATVDNI
                     VATWPGQGERIVMLGGHLDSVLDGPGINDNGSGVATLLALAASVAAQPPAQSTIQFGF
                     WSAEEFGDLGSFAYVDSLGPDERGLIDAYLNLDMVGSPNPGRYVYDEPTSPQGSYELT
                     TSLLAAFEALGAPAGAIDTGGSSDHYAFALAGIPVGGVFSGLAPMTPQDAELFGGVAN
                     EPEDPCYHLACDTRGNVDLDSAVLLGQAVANLLMELAY"
     gene            complement(1483..3009)
                     /locus_tag="VIF08_05945"
     CDS             complement(1483..3009)
                     /locus_tag="VIF08_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HEY8135564.1"
                     /translation="MTQDESSAALEGTPADPTPNQPFVDTPVERETLATPAMGLEPRE
                     QPGKRVIIIGGGIAGLVAAFELMRQGHEPLILEAQHRVGGRVRTIRDFAPGLYAEAGA
                     MRIPRVHDLTLAYCDLFGLQLRPFVMGNPNTLVYVNGQRMTVAEADANPDKLPFDLAD
                     HERGKTWTQLWNEATSEFRDQYEKGGVESLDSLLREYDQYSIREFLVMRGFSEGALEL
                     YGVMSFREANMNAAVVEQLREIVGRSFEDMQEIVGGLDLLPRAFYENLKPHVRFGAEV
                     TAIEQSPDSVTVHYKQGPAGRMSVTGDYAICAIPFSVLRDIEILNTPFSRPKQKAIRE
                     LNYNASAKILFQVRHRFWEDEDGIVGGTTVTDLPIRRICYPSYSDPNDERGTLLASYT
                     WGQDALRWGAMSPSDMIEQALEDVAQVHPSVLKEFEVGAVQDWYGDPYARGAFALFEP
                     EQQTRLQEAIVAPEGRIHFAGEHTSLYHAWIQGALESGIRAAREVHEAPPVFAGAAEG
                     "
     gene            complement(3042..3866)
                     /gene="rsmI"
                     /locus_tag="VIF08_05950"
     CDS             complement(3042..3866)
                     /gene="rsmI"
                     /locus_tag="VIF08_05950"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY8135565.1"
                     /translation="MATLYVVATPIGNLADVSQRALDVLAAVPLVVAEDTRVTRRLLA
                     RYKIETRLESFHAHSGASRVAALVEHLAGGKDLALTSDAGTPLVSDPGEDLVAAWAAR
                     GGTIVPIPGPSAALAALVASALPAARFAFEGFLPRRGKERRERLAAIASDNRASIIFE
                     APGRAAATLADLARVCGADRRASLSRELTKLHEETWRGTLAELAARAEAEAPRGEVTL
                     VISGAAASQGEPTTTLDIGRREVDRLVGEGMSRSSAAREVAQRTGLARRELFRAAG"
     gene            complement(3863..>4227)
                     /gene="nadE"
                     /locus_tag="VIF08_05955"
     CDS             complement(3863..>4227)
                     /gene="nadE"
                     /locus_tag="VIF08_05955"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEY8135566.1"
                     /translation="NAAAFLPIGDLYKSQVRQLAADMGVPAAILEKPPSADLWPGQTD
                     EGELGMTYADMDRLLYWLVDRRRTRDQLEAMGFGASHIERVERLIAGSEFKRQTPVVA
                     KLTTRTPGVDYLYPRRRP"
BASE COUNT          622 a         1482 c         1444 g          679 t
ORIGIN      
        1 ctgggcaccg tcatcgtggt cattctcgcg ggctgcacga cgagcccggt ggtaccgact
       61 gccagcccga tcccgaccgt tgccccgatg ggcacaccca gttcactgcc cagcaacccc
      121 gccagcgcga cgccgaccgc agcggccagc gccacagcct ccatcgtgcc gtccgttgca
      181 ccgaccgggt cgccgaccgg cctgcaggtg aacgggagcc tcatcaccgg ccatctcgat
      241 gcgctccaag cgatcgccga cgccaacggc ggcatccgag ccgcgggcac gccgggctac
      301 gacgcgtccg ccgactacgt tgcgcaggtg atgacggatc tcggctacca gctcgagcgc
      361 caccccttcg acttcccgtt cttcaacgag accgcgccgg cgtcggttgg cttcgccgac
      421 gtcgcctgga ccgggtccga atgggttcac gcggcgctct attcggccag cggcgacgtc
      481 gaggcgcagc tcgagctcgt cggcgacggc ctggggacgt tcggctgcga tgccagcgaa
      541 tggtcgggct tcacgtctgg ccacattgcc ggggtctacg gcggcgggtg ctttatgcgc
      601 cagaaagtcg agctcgcgca ggatgccggc gcggtggcga tgatctcgat gtatccgacc
      661 tgggagcaga acgagatcaa gcggccgacg ttgctcgatc cagacggaat atcgattccg
      721 gtggttgtcg ccggtcgcga gccagcgctg gccctggcga accaggaggg caacatggcc
      781 agcgtcttcg ttcagggcga caaccacatt gcgacggtcg acaacatcgt cgccacgtgg
      841 cccggccagg gcgagcgcat cgtgatgctc ggcggccacc tcgactcggt gctcgacggc
      901 ccgggcatca acgacaacgg ctctggcgtc gcgacgctgc tggcactcgc ggcttcagtc
      961 gcggcccagc cgccggcgca gtcgaccatc cagttcggct tctggtcggc cgaggagttc
     1021 ggtgacctgg gctcgttcgc ctatgtcgac tcgctcggtc ccgatgagcg cggcctgatc
     1081 gacgcctatc tcaatctcga catggtcggt tcgcccaacc ccggccggta cgtgtacgac
     1141 gaacccacct ccccccaggg ctcgtacgag ctcaccacga gcctgctcgc ggcgttcgag
     1201 gcgctgggcg cgccggcggg cgcgatcgac accggcggct catccgacca ctacgcgttc
     1261 gccctcgccg ggatccccgt gggtggtgtg ttctccggtc tcgccccgat gaccccgcaa
     1321 gacgccgagt tgtttggtgg cgtcgcgaac gagccagaag acccctgcta ccacctcgcc
     1381 tgcgacacga gaggcaacgt cgatctcgac agcgccgtgc tgctcggcca ggcggtggcc
     1441 aacctgctca tggagctcgc ctattaggtt cggggttcgg ttctagccct cggcggcccc
     1501 ggcgaaaaca ggcggcgcct cgtgcacctc gcgcgccgcg cggatgcctg actcgagcgc
     1561 cccctggatc cacgcgtggt acaacgaagt gtgctcaccc gcgaagtgga tgcggccctc
     1621 gggcgcgacg atcgcttcct gcaggcgcgt ctgctgctcg ggctcgaaca gcgcgaacgc
     1681 gccgcgggcg tagggatcgc cgtaccaatc ctggacggcg cccacctcga actccttcag
     1741 caccgacgga tgcacctggg cgacgtcctc gagcgcctgc tcgatcatgt cgctcgggct
     1801 catcgcgccc cagcgcagcg cgtcctggcc ccacgtgtac gacgcgagca gcgtgccgcg
     1861 ctcgtcgttg gggtcgctgt atgacgggta gcagatccgc cggatgggca ggtcggtgac
     1921 ggtcgtgccg ccgacgatgc cgtcctcgtc ctcccagaag cggtggcgca cctggaacag
     1981 gatcttggcc gacgcgttgt agttgagctc gcggatggcc ttctgcttgg gcctggaaaa
     2041 cggcgtgttg aggatctcga tgtcgcgcag gacgctgaac gggatggcgc agatggcgta
     2101 gtcgcccgtg acggacatgc gcccggccgg cccctgcttg tagtggaccg tcacggaatc
     2161 gggtgactgc tcgatcgcgg tcacctccgc gccaaagcgg acgtgcggct tgaggttctc
     2221 gtagaaggcg cgcggcagca ggtccaggcc gcccacgatc tcctgcatgt cctcaaagct
     2281 gcgaccgacg atctcgcgca gctgctcgac cacggccgcg ttcatgttcg cctcgcggaa
     2341 gctcatgacg ccatagagct cgagcgcgcc ctcgctgaag ccgcgcatca cgaggaactc
     2401 gcgaatcgaa tactggtcgt actcgcggag cagtgagtcg agcgactcca ccccgccctt
     2461 ctcgtactgg tcgcggaact cgctcgtcgc ctcgttccag agctgtgtcc acgtcttgcc
     2521 ccgctcgtga tcggccaggt cgaacggcag cttgtcagga ttggcgtcag cctccgcgac
     2581 ggtcatgcgc tggccgttga cgtagacgag cgtgtttggg ttgcccatca cgaacggccg
     2641 cagctggagc ccgaacaggt cgcaataggc gagcgtcagg tcgtgcacgc gcgggatgcg
     2701 catcgcgccg gcctcggcgt atagcccggg cgcgaaatcg cgaatggtgc ggacgcgccc
     2761 accaacgcgg tgctgcgcct ccaggatgag cggctcgtgc ccctggcgca tgagctcgaa
     2821 cgcggccacc agcccggcga tgccgccgcc gatgatgatc acccgcttgc ccggctgctc
     2881 gcgcggctcc aggcccatcg cgggcgtggc gagggtctca cgctcgaccg gggtatcgac
     2941 gaacggctgg ttgggcgttg gatccgccgg cgttccctcg agagccgcgg aagactcgtc
     3001 ctgggtcacg gtgcctccac gttggaaact agtccgcgag tctatccggc cgcgcggaac
     3061 aattcgcggc gcgcgagacc agtccgctgg gccacttcgc gggcggccga ggagcggctc
     3121 atgccctccc cgaccaggcg atccacctcc cgccgaccga tatccagagt cgtcgtgggc
     3181 tcaccctggg aggctgcagc gccactgatc accagggtca cctcgccccg gggtgcttcc
     3241 gcctctgccc gggccgcgag ctcggccagt gtcccgcgcc aggtctcctc gtgcagcttg
     3301 gtcagctcgc ggctcaggct cgcgcgacgg tcggcgccgc atacccgggc aaggtcggcg
     3361 agggtggccg cggcgcggcc gggcgcctcg aagatgatcg aggcccggtt gtcgctggcg
     3421 attgcggcca ggcgttcgcg gcgctccttg ccgcgccgcg gcagaaagcc ctcaaacgcg
     3481 aaccgcgcgg caggcaacgc actcgccacg agcgcggcca gcgcggccga cggcccgggt
     3541 atgggcacga tcgtgccgcc ccgtgcggcc caggccgcta cgaggtcctc gcccggatcg
     3601 ctgacgagtg gcgtgccggc atcgcttgtc agggcgaggt ccttgccccc cgcgaggtgc
     3661 tccacgaggg cggccacgcg cgaggcaccg gagtgtgcgt ggaaagactc gagccgcgtc
     3721 tcgatcttgt accgcgccag cagccggcgc gtgacgcggg tgtcctcggc cacgaccagc
     3781 ggcactgccg cgaggacgtc gagggctcgt tgcgacacgt cggcgagatt gccgatgggc
     3841 gttgcgacga cgtacagcgt ggctatggcc ggcgccgcgg gtacaggtag tcgacgcccg
     3901 gtgtgcgcgt cgtgagcttg gcgaccaccg gcgtctggcg cttgaactcg gacccggcga
     3961 tcaggcgctc gacgcgctcg atgtgtgacg cgccaaagcc catcgcctcg agctggtcgc
     4021 gcgtccgccg ccggtcgacc agccagtaga gcaagcggtc catgtcggcg taggtcatgc
     4081 ccagctcgcc ctcgtcggtc tggcctggcc acaggtcggc gctcggcggc ttctcaagga
     4141 tggctgccgg gacacccatg tcggcggcaa gttggcggac ctggctcttg tagaggtcgc
     4201 cgatgggcag gaacgccgcc gcgttgt
//
LOCUS       DATBRX010000155         6295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3074272, whole genome shotgun sequence.
ACCESSION   DATBRX010000155 DATBRX010000000
VERSION     DATBRX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074272"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VIF08_05960"
     CDS             complement(<1..181)
                     /locus_tag="VIF08_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135567.1"
                     /translation="MVGAAGLAIAGRLIARRAQRSAHLGLVDWPRVEQLAVNRLRNVP
                     GALPRSELVAAQGEYS"
     gene            complement(256..1074)
                     /gene="murI"
                     /locus_tag="VIF08_05965"
     CDS             complement(256..1074)
                     /gene="murI"
                     /locus_tag="VIF08_05965"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY8135568.1"
                     /translation="MSDPRSIGFFDSGVGGLTVLREVVRRLPNESTIYIGDNARAPYG
                     PRTDDEVKRFSAECLDELASRDVKAIVVACNTSSAVALPDLRLRYNMPLLGVVRPGAT
                     AAALATRNRRVGVIGTAATIRSHAYFQAIKEEDPFVEVIEQATPDLVPLVEAGHLEGP
                     LVEDVVRRSIAPITDSDRDPDTLLLGCTHYPLLASIFREIVGPDVAVIDTASATASAL
                     AHLLEMHDLNAPADVTPKHVQLTTGDIGAFSVIAERLFGAEFARVEPVAVAGAA"
     gene            complement(1093..2304)
                     /locus_tag="VIF08_05970"
     CDS             complement(1093..2304)
                     /locus_tag="VIF08_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8135569.1"
                     /translation="MSGPPSDAQIRLLLVEDMPQVAQYIRGLLDTQTRIKLLDVVTDG
                     RIVIEQVREHQPDVLIVDALLQGKINGLQVAADVREAGMELPIVCLTVPGKPIAIGDG
                     MGETRVLAMPFSGYDFMHLLQQMTAEHRARAPEQMSRVYAMFGAKGGVGTTTLAYNIG
                     AALAAQGLTVALLDGSLQFGDLRSLLRVPEATPSIVQLPTNRLQRSELQDAMYRDKSG
                     VEVLFAPPRIELAEMVTVRDLERLISLMRKVYNVVLIDTATTVDDTLLAYLDASDELI
                     QVLTYEWPSLHRARAMSDTLNAINYPDSRVRYLANRADSKGGLSRDDIMTTLGRQPDF
                     EVVSDGVLVLEANNRGEPFMTLNPKARVSGDVESIAKALVRSMQEEHRAAADTAAREA
                     MAREQASTADS"
     gene            complement(2346..3143)
                     /locus_tag="VIF08_05975"
     CDS             complement(2346..3143)
                     /locus_tag="VIF08_05975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8135570.1"
                     /translation="MRGRRLTEERQPVEAWSRAADLARYYDLDLLTNPGDLPFYLDLA
                     ASSASPILELAVGSGRLAVPLAAAGHDVTGVDNAATMLGRARAAWEETIQPLGTLTLL
                     EHDLTTLSLPARFDLVILALNSLLLLDGRAAQARALQVMRAHLAPTGRAVIDVWLPGG
                     DDLALYDGRQVLDWVRTDPRTGERVAKSWSATYDAAARAATVNSTFETEAGSRATRRD
                     VISFISTQELLELAQAAGLEPESVLGDYSGTIWSDASERVVLIAGGG"
     gene            complement(3125..4231)
                     /gene="speB"
                     /locus_tag="VIF08_05980"
     CDS             complement(3125..4231)
                     /gene="speB"
                     /locus_tag="VIF08_05980"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY8135571.1"
                     /translation="MTDKHAPPDFAQVNGWDKPYELFLPPEVPAYTGVSLSFCHRPLI
                     EDADELARRAPDVAIVGAPVDEAVSYRPGARFGPRAIRQASYTQGGYSLQLGIRPFDH
                     VDVVDAGDANVAPAALDRNHAMVYRKVLEVARTGAVPIVLGGDHSITWPSASAVAAAR
                     HPRSVGIVHFDAHADAANDDWGVLAGHGTPMRRLIESGAIDGKNFVQVGLRGYWPPPE
                     VFAWMKEHGLRWHLMREIEERGAEAVIDDAIAEALDGPDAIYLSIDIDVLDPGNAPGT
                     GTPEPGGMLTRELLRAIRRIVGKVSLAGMDIVEVSPPYDHADVTAAAAHRCALEAISA
                     LAVRKRDGLPVRFEAQDVAMPTAGPEVHASGAGG"
     gene            complement(4312..4686)
                     /locus_tag="VIF08_05985"
     CDS             complement(4312..4686)
                     /locus_tag="VIF08_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135572.1"
                     /translation="MNRADRLTNVGLLTVALAAWVAVALIMVTFDPRANASALLAGAL
                     TLGAALTGTLTPLLWLVGFALQGRIAYRGDWWRAARRAALVGLIVAIFIVLRGEQALN
                     LPLALFVVAMAVLVELTLSLRR"
     gene            complement(4683..5225)
                     /locus_tag="VIF08_05990"
     CDS             complement(4683..5225)
                     /locus_tag="VIF08_05990"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HEY8135573.1"
                     /translation="MIVYRHADPRFPFLWESSAQPPARWHGVGEGPVQYFSSTPDAAW
                     AEFLRHEEITDAADVATVTRSLWAIELPDEPLAAPDLPDATLVGGTSTYPACRAQASV
                     LRAAGAGGLVAPSAALDRRTGSGYRTDAGLRPGPRREESVVVLFGARPDLVGWAACFE
                     GRPRPELIGRVRQLRTESWS"
     gene            complement(5222..5845)
                     /locus_tag="VIF08_05995"
     CDS             complement(5222..5845)
                     /locus_tag="VIF08_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135574.1"
                     /translation="MTVIRIASIDAPLNSAGLRGPALDLVRRALAVGLLRDRPLIGKL
                     DLDLLRGIAREASAAGVGRDAALELLGKPSPRHLGSVIGRLEDALASSPMPEHELKVL
                     LEVFDVDLLATLLDTSAVSLRRYITGARTVPDAVAARIHWLALVSSDLAGAYNALGIR
                     RWFDRPRTQLDGKSPRQLLGRGWNPDGAKAERVRALAAALAGPGGAA"
     gene            complement(5882..>6295)
                     /locus_tag="VIF08_06000"
     CDS             complement(5882..>6295)
                     /locus_tag="VIF08_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY8135575.1"
                     /translation="VVAVERPFEFALDGMRIRGRVDRLDEDEGGTVIVDYKTSDVRDQ
                     RKANDKARGSLQLHAYALAHEQKTGKRPRAMQLHFLESSLIGESTPDPARLDKAREQL
                     RAAMAGIAAGEFAPQPNPVACGFCPFRQICVASAA"
BASE COUNT         1004 a         2149 c         2209 g          933 t
ORIGIN      
        1 ggctgtattc accttgcgcg gcaacaagct cggagcgcgg caacgcgccg ggcacgttac
       61 gcaggcggtt gacggcgagc tgctcgacac gcggccagtc gaccaggccc aggtgcgcgc
      121 tcctctgagc acgccgcgca atcagccggc cggcgatggc caggccggcg gcgccaacca
      181 cgacacccag tcccagcgcg gcctctgccc gtgaagaccg ctgtgcgggt cggcgagggc
      241 cctgctgcgt cgcggtcacg cggccccagc cacggcgacc ggctcgacgc gcgcgaactc
      301 ggcgccgaac agccgctcgg caatgacgct gaacgcaccg atgtcaccgg ttgtcagctg
      361 cacgtgcttg ggagtcacat ctgcgggtgc gttgaggtcg tgcatctcga gcaggtgggc
      421 aagggcactc gccgtcgcgc tggctgtatc gatcacggcc acgtcggggc cgacgatctc
      481 gcgaaagatc gatgccagga gcggatagtg ggtgcagccc aggaggagcg tgtccggatc
      541 gcggtccgaa tcggtgatcg gagcgatgga tcggcggacg acgtcctcga ccagcggccc
      601 ctcgaggtgg ccggcttcga cgagcggcac gaggtcgggc gtggcctgct cgatcacctc
      661 gacgaacggg tcctcttcct tgatcgcctg gaaataggcg tgcgacctta tcgtcgccgc
      721 cgtgccgatg acgccaacac gccggttgcg cgtcgcgagc gcggccgcgg ttgcgccggg
      781 ccggaccacg ccgagcagcg gcatgttgta gcgcagccgc agatcaggca gggcgacggc
      841 actcgaggtg ttgcaggcga cgacgatggc cttgacgtcg cgtgacgcca gctcatcgag
      901 gcattccgcg gagaagcgct tgacctcgtc gtcggtgcgc ggaccgtacg gcgcacgcgc
      961 gttgtcgccg atgtagatcg tcgactcgtt cggtagacgc cggaccacct cgcgcaacac
     1021 ggtcaggcca cccacgcccg agtcgaagaa gccgatcgat cgcgggtcgc tcatgacctg
     1081 ctcatggcgc cctcatgaat cggcagtcga cgcctgctcg cgggccatcg cctcgcgtgc
     1141 cgccgtgtcg gcagcggcac gatgctcctc ctgcatcgag cgcaccagcg ccttggcaat
     1201 cgattcgacg tcgccgctca cccgtgcctt ggggttgagc gtcatgaatg gctcgccacg
     1261 attgttggcc tcgagcacca ggacgccatc gctgacgacc tcgaagtcgg gctggcgacc
     1321 gagcgtcgtc atgatgtcgt cgcgcgacag accgcccttg gagtcggcgc ggttggccag
     1381 atagcgcact cgtgaatcgg gatagttgat ggcattgagc gtgtcgctca tggcgcgcgc
     1441 gcgatgcagt gacggccatt cgtaggtcag gacctggatc aattcgtcgg acgcgtccag
     1501 gtacgcgagc agggtgtcgt cgacggttgt cgcggtgtcg atcagaacga cgttgtagac
     1561 cttgcgcatc aatgagatga gacgttccag atcgcgcact gtgaccatct cggcgagttc
     1621 gatacgcggt ggagcgaaga gaacctccac gcccgacttg tcgcggtaca tggcgtcctg
     1681 cagctccgag cgctgcagac ggttggtcgg cagctgcacg atggacggcg tggcctcggg
     1741 cacgcgcagc aggctgcgca ggtcaccgaa ctggaggctg ccatccagga gcgcgacggt
     1801 caggccctgc gccgccagcg ccgcgccgat gttgtaggcc agcgtcgtcg taccgacgcc
     1861 gcccttcgcg ccgaacatcg cgtagacgcg cgacatctgc tcgggcgcgc gtgcccggtg
     1921 ctcggcggtc atctgctgca agagatgcat gaagtcgtag ccggagaaag gcatcgcgag
     1981 gacgcgcgtc tcgcccatcc cgtcaccgat cgcgatgggc ttcccgggca ccgtcaggca
     2041 gacgatgggc agctccatgc cggcctcgcg gacgtcggcc gcgacctgta ggccgttgat
     2101 cttgccctgc agcagcgcgt cgacgatgag gacgtcgggt tggtgctcgc gtacctgctc
     2161 gatgacgata cggccgtcgg tcacgacgtc gagcagcttg atccgcgtct gagtgtcgag
     2221 caggccgcgg atgtactgcg ccacctgcgg catgtcttcg accagcagca ggcggatctg
     2281 tgcgtcagat ggggggccag acaccggccg actctatatc aagaggccgc tgcaggggac
     2341 tgggactacc cgcccccggc gatcaggacg acccgctcac ttgcgtccga ccagatcgtg
     2401 ccggaatagt caccgaggac cgattcaggc tccaggccag ccgcttgcgc aagctcgagg
     2461 agctcttgcg tgctgatgaa gctgatcacg tcccggcgcg tcgcgcgact gccggcttcg
     2521 gtttcaaacg tcgagttcac ggtcgccgcg cgtgcagccg cgtcgtaggt ggccgaccac
     2581 gacttcgcca cgcgttcgcc ggtccgaggg tcggtgcgga cccagtcgag cacctggcgc
     2641 ccgtcgtaca gcgccaggtc gtccccgccc ggcagccaca cgtcgatcac ggcgcggccc
     2701 gtgggcgcca ggtgcgcgcg catcacctgc agggcgcgtg cctgcgccgc gcggccgtcg
     2761 agcagcagca ggctgttgag cgccaggatc acgaggtcga agcgcgccgg caggctgagc
     2821 gtcgtcaggt cgtgctcgag cagcgtcaga gtgccgagcg gctgaatcgt ctcttcccac
     2881 gccgcgcgtg cccgcccgag catcgtcgcg gcgttgtcga cgccggtcac gtcgtgaccg
     2941 gccgcggcca gcggaaccgc aagacggccg ctgcccaccg cgagctccag gatggggctg
     3001 gcgcttgacg cggcgagatc caggtagaag ggcaggtcgc ccgggttcgt gagcaggtcc
     3061 aggtcgtagt agcgcgccag gtccgcggcg cgcgaccatg cctcgactgg ctgcctctcc
     3121 tccgtcagcc gccggccccg gatgcgtgga cttccggtcc ggcggtgggc attgccacgt
     3181 cttgcgcctc aaagcgcacg ggcagcccgt cccgcttgcg cacggcgagg gcgctgatcg
     3241 cctccagcgc gcagcggtga gcggctgcgg cagtcacgtc ggcatggtcg tatggcggcg
     3301 agacctcgac gatgtccatg ccggccaggg acaccttgcc cacgatacgt cgaatggcgc
     3361 gcagcagctc gcgcgtgagc atgccgcccg gctcgggcgt gcccgttcct ggggcattgc
     3421 ccggatcaag cacgtcgata tcgatcgaca ggtagatcgc gtccgggccg tcgagcgcct
     3481 cggcgatcgc gtcgtcgatg acggcctccg cgccgcgctc ctcgatctcg cgcatgaggt
     3541 gccagcgcag gccgtgctcc ttcatccagg cgaatacctc gggcggcggc caatagccgc
     3601 gcagccccac ctgcacgaag ttcttgccgt cgatggcgcc tgactcgatg agccggcgca
     3661 tgggtgtgcc gtggcccgcg agcacgcccc aatcgtcgtt cgccgcatcg gcgtgggcgt
     3721 cgaagtgaac gatgccgacg ctgcgcggat ggcgcgcggc ggcgacggcg ctcgccgatg
     3781 gccaggtgat cgagtggtcc ccgccgagga ctatcggcac agcgccggtg cgcgcgacct
     3841 cgagcacctt gcgatagacc attgcgtggt tgcggtcgag cgcggccggc gccacgttgg
     3901 cgtcgccggc gtcgacgacg tcgacgtggt cgaacggccg gatcccgagc tggagcgagt
     3961 agccgccctg ggtgtacgac gcctggcgga tggcgcgcgg cccgaatctc gcgccggggc
     4021 ggtagctgac cgcctcgtcg acgggcgcgc cgacgatggc cacgtcgggc gcacgtcggg
     4081 caagctcatc cgcgtcctcg atgagcggtc ggtggcagaa ggacaggctg acgcctgtgt
     4141 atgccggaac ttcgggcggc aggaaaagtt cgtatggctt gtcccagccg ttcacttgcg
     4201 cgaagtcagg cggagcgtgc ttatcggtca cttgcacgca gtcctcgggc ggagtcgccg
     4261 ccggttcccg cagaggtcca gcagggcgcc cgcgggcatg ttagccgacg cctagcggcg
     4321 tagcgagagc gtcagctcga ccagcacggc catggcgacc acgaagagcg cgagcggcag
     4381 gttgagcgcc tgctcaccgc gcagcacgat aaagatggcg acgatcaagc cgaccagcgc
     4441 cgcgcgccgc gcggcgcgcc accaatcgcc gcggtacgcg atgcggccct gcagcgcgaa
     4501 gccgacgagc cacagcagcg gcgtgagcgt gcctgtaaga gcagcgccca gcgtcagcgc
     4561 gcccgccagc agagctgacg cgttggcgcg cggatcaaac gtcaccatga tgagtgctac
     4621 tgcgacccac gccgcgaggg caacggtcag caaaccgacg ttggtcaggc gatcagctcg
     4681 attcacgacc aggactccgt gcgcagctga cgcacgcggc cgatgagctc gggccgcggc
     4741 cggccctcga agcacgccgc ccatccgacg aggtcaggcc gcgcgccgaa cagcacgacg
     4801 acggactcct cgcgccgcgg cccaggacgc aggccggcgt ccgttcgata gcccgagccg
     4861 gtacgccgat cgagcgccgc tgatggcgcc acgagtcccc cggcgccggc cgcacgcaga
     4921 acactcgcct gggcgcggca tgccggatag gtggacgtgc cgccgacgag cgtcgcgtcg
     4981 ggcaggtccg gcgccgcgag gggctcgtcg ggcagctcga tcgcccacag cgaccgggtg
     5041 acggtcgcca catcggccgc atccgtgatc tcctcgtggc gcaggaactc ggcccacgcc
     5101 gcgtccggcg tcgaggagaa gtactggacc gggccctccc cgacgccgtg ccagcgggcc
     5161 gggggctgag cgctggactc ccacaggaac gggaagcgtg ggtcggcgtg gcgatacacg
     5221 atcatgccgc gccaccagga ccggcaaggg ccgccgcgag ggcacggacc cgttcggctt
     5281 tcgcgccatc agggttccag ccacgcccca gaagctggcg cggcgacttg ccatcgagct
     5341 gcgtgcgcgg ccgatcgaac cagcgccgga tgccgagcgc gttgtaggca ccggccaggt
     5401 ccgagctgac caaagcgagc cagtggatgc gcgccgccac ggcatcaggc acggtgcgcg
     5461 ccccagtgat gtagcgccgc aggctcaccg ccgacgtgtc gagtagcgtc gcgagcaggt
     5521 caacgtcaaa aacctcgagc agcaccttga gctcatgctc cggcatgggc gatgaggcca
     5581 gcgcatcctc gagccggccg atgacactgc ccaggtggcg cggcgacggc ttgcccagga
     5641 gctcgagtgc cgcatcgcgc ccaacacccg ccgcggatgc ctcgcgggcg atgccgcgca
     5701 acaggtcaag gtcaagcttg ccgatgagcg gccggtcacg cagcaggccg accgcgagag
     5761 cgcgccgcac gaggtcgagc gccggtccgc gtaacccggc cgagttgagc ggtgcgtcaa
     5821 tggacgctat tcggatcacc gtcacgatcc atattgtatc cgcacggcgc aagcgcgggc
     5881 gtcaagcggc gctcgcaacg cagatctggc gaaacgggca gaagccgcac gcaactggat
     5941 tgggctgcgg cgcaaactcg ccagccgcga tgccggccat cgctgcgcgc agctgttcgc
     6001 gcgccttgtc cagccgggct ggatccggcg tgctctcgcc gatcaggctg ctctcaagga
     6061 agtggagctg cattgcccgc ggacgcttgc cggtcttctg ttcatgcgct aaggcgtagg
     6121 cgtgcagctg gagcgaaccg cgcgccttgt cattcgcctt gcgctggtcg cgcacgtccg
     6181 acgtcttgta gtcgacgatg actgttccgc cttcgtcctc gtccaggcga tcgacacggc
     6241 cgcggatgcg cataccgtcg agcgcgaact cgaagggtcg ctccactgcg accac
//
LOCUS       DATBRX010000156         6456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2962927, whole genome shotgun sequence.
ACCESSION   DATBRX010000156 DATBRX010000000
VERSION     DATBRX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962927"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..521
                     /locus_tag="VIF08_06005"
     CDS             36..521
                     /locus_tag="VIF08_06005"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY8135576.1"
                     /translation="MSTFTESPTLDRIDRRITRAMARHGLLLMRLALGVVFLWFGVLK
                     FFPGLSPAETLAGKTIEALSLGYLSASTAVLILAAWECLIGLGLLTGLFMRATLALLF
                     LQMLGTLTPLVLYPAETFIAFPYAPTLEGQYIIKNAVLIAGAVILGATVRGGRIESEP
                     G"
     gene            complement(537..1217)
                     /gene="msrA"
                     /locus_tag="VIF08_06010"
     CDS             complement(537..1217)
                     /gene="msrA"
                     /locus_tag="VIF08_06010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY8135577.1"
                     /translation="MFLFNRGKSAMPSAVNALPGRSEPILPNPRPHRVLGTPIAGPYP
                     DGFQVAEFALGCFWGEEKTFWEQPGVWTTAVGYQGGYTPNPTYQESCTGQTGHAETVR
                     VVFDPAKTSYEKLLKVFWENHDPTQGMRQGNDVGSHYRSAIFVHDEAQRAAAEASKQM
                     YQQQLSARGYTEITTEIVGPPAPPFYLAEDYHQQYLEVNPGGYCPNHATGIRLPDGWA
                     VTPLEYAE"
     gene            1313..2068
                     /locus_tag="VIF08_06015"
     CDS             1313..2068
                     /locus_tag="VIF08_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEY8135578.1"
                     /translation="MKTGEMLRATPRRLVTPMVLAVLAGVVLGGALAVPSPAVAAQDL
                     AGIDVSHWQGQITWSDVAADGVSFVIAKATEGRVYQDDQYARNKERAEANGIAFTAYH
                     FASPDKTANDAILEADNFVDTADLGPGNLRPVLDLEKNGGLGVRKLRAWVQAWLARVE
                     DRLGVKPIIYTSPSFWHDKMGNSRWFANNGYALWIAHWHVATPRVPARNWGGRGWTIW
                     QYDDCGSVAGIDGCVDLDRLNGKDIDALRIPGG"
     gene            complement(2082..2363)
                     /locus_tag="VIF08_06020"
     CDS             complement(2082..2363)
                     /locus_tag="VIF08_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135579.1"
                     /translation="MQEDDSVRIAWLADGFVAIHVYARFWPTTEVAQRAAEVADICLT
                     NRTLHDLRRGLRQRFGSGFILQAPDEDAPDRRVAVFFDPPVGPPNPDEI"
     gene            2373..3416
                     /locus_tag="VIF08_06025"
     CDS             2373..3416
                     /locus_tag="VIF08_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY8135580.1"
                     /translation="MPLVAALAVLLTPASAAAAGEPAGYGYFHTYAEVKAQIDDTVAA
                     HPGIVQRFSMGTSYEGRPIWGVKVSDNVGSDEGEPEVFVVAQLHANERASGEMALYLM
                     DLLVDGYGSDSRITDIVNSREIWIVPVANPDGAEYDMSDGVFRRWRKNRQPIPGSSYV
                     GIDLNRQFGFKWNCCGGGSNNPSSPKYRGTEPWQAPETRAYRDFILSRVVGGQQQLAI
                     ALSIHAQGRLVLWPYAYTKKDHPPTMRYDDWRAFVALGRDVAALNGYRARQTSDLYVV
                     DGDEGDWAYHAQGIFAFAFELAAGSPERMYPTLAQLQADQARNRPAVLLLLEAADCPY
                     AAVGAALAAKHCS"
     gene            3413..5041
                     /locus_tag="VIF08_06030"
     CDS             3413..5041
                     /locus_tag="VIF08_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY8135581.1"
                     /translation="MSRPLRTRAFAVLLATLACAVMPGSAIAANAFPAGHEGYHNYAE
                     MSAFLDDVAAAHPGIAEKFSMGRSYQGRQVWALKISDNVGTDENEPEVLFTSQMHARE
                     WLSLEQDLRIIELLTSNYRANPGTQLEGRVKDIVDGLEIWIVPMLNPDGAEYDISNPS
                     GGFRNQRKNRQPVGDGVQPGIDLNRNFGFNWACCGGSSGKPGSFYYRGRAPWQGVETS
                     LLRDFVLGRIVGGRQQIRAAIDWHAFNEEIMWPFAYTKADLPRPMAADDLQAFKAIAR
                     GMADLNGYMPKQLSDLYVFDGGSTDWLYGDQRIFSLTIEVYPLDKSHPGFYPPDSIIE
                     RETTRNDGAVLYFLEQVACPYRAAGLDDTHCGPLDDDFETGRGWTVDPLDTDTATGGA
                     WQRAIPAKSKTSAGQKQRAYGFSARYALVTGAARGASANANDLDGLTSVRSPQFDLGG
                     SSSSGWRVQFRYTFAHAANSSSADYLRLLVDGQEVWRSSGAASERKASWGLATVSLDA
                     YAGQTVRLLFEACDCAATSLVEAVVDDVRVYKVP"
     gene            5118..5285
                     /locus_tag="VIF08_06035"
     CDS             5118..5285
                     /locus_tag="VIF08_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135582.1"
                     /translation="MTNDRKRHLTWPDRRTSETATSRVLPPTADQSAGFSNLATQPRA
                     GNISSDTRAGY"
     gene            complement(5495..>6456)
                     /locus_tag="VIF08_06040"
     CDS             complement(5495..>6456)
                     /locus_tag="VIF08_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8135583.1"
                     /translation="SLTARLRERRPRFVLIAARGTSDNAALYAQYLFAIRNSLPVALA
                     APSTITLYGARPDMRDALVVGISQSGRSPDIVAVLDEARGQGALTVAMTNDAASALAA
                     AADEVIDLRAGPERATAATKTYTTELLAVALLSTALDGASAHETADLARLPELVAAAL
                     AAEDAARSVSAGHALRTHGVVLGRGFAYATAREWSLKLQELAHVLVQPYSTADFEHGP
                     LALAEPGFAVLAVAPSGVAYEGQLEVLARLRQEFGARLLVISDVAEMLALDEGLPLPS
                     DVPGWLSPIVEIVPGQLYAYHLTIARGLDPDRPRSISKVTETL"
BASE COUNT         1036 a         2200 c         2223 g          997 t
ORIGIN      
        1 ggatgagctc tgccacaaga gtcgaaaatg gaaggatgag caccttcacc gaatcgccca
       61 ctctcgaccg tattgaccgg cgaatcaccc gcgccatggc ccgccacggg ttgctgctga
      121 tgcgcctcgc cctgggggtc gtgttcctgt ggttcggggt gctcaagttc ttcccgggcc
      181 tgagcccggc cgagactctt gccggcaaga cgatcgaggc actgtcgctg ggctaccttt
      241 cggcttcgac ggccgtcctg atcctggccg cctgggagtg cctgatcggc ctgggcctgc
      301 tcaccgggct cttcatgcgc gccaccctgg ccctgctgtt cctgcagatg ctcggcaccc
      361 tgacgccgct tgtgctctat ccggccgaga cgttcatcgc cttcccgtac gcgcccacgc
      421 tcgagggcca gtacatcatc aagaacgcgg tgctcatcgc cggcgcggtg atcctgggtg
      481 caacggtccg cggcggccgg atcgagagcg agccgggctg acccgagcgc ggggtcttac
      541 tccgcgtact cgagcggcgt aacggcccag ccgtcgggca accgaatgcc cgtcgcgtgg
      601 ttggggcagt agccgcccgg gttcacctcg agatactgct ggtggtagtc ctcggccaag
      661 tagaacggcg gcgccggcgg gccgacgatc tcggtcgtga tctctgtgta gccgcgggcg
      721 ctcagctgct gctggtacat ctgctttgac gcctctgcgg ccgcccgctg cgcctcgtcg
      781 tggacgaaga tcgccgagcg gtagtgcgag ccgacgtcgt tgccctggcg catgccctgc
      841 gtcgggtcgt ggttctccca gaagaccttg agcagcttct cgtagctggt cttggccggg
      901 tcgaacacga cgcgcaccgt ctcggcgtgg ccggtctggc cggtgcacga ctcctggtag
      961 gtgggattgg gcgtgtaccc gccctggtag cccacggccg tggtccagac gccgggctgc
     1021 tcccagaacg tcttctcctc gccccagaag cagccgagag cgaactcggc gacctggaaa
     1081 ccgtcgggat acggccccgc gatgggcgtg ccgaggacgc gatggggtcg cgggttgggc
     1141 agtatcggct cgctccggcc cggcagcgcg tttacggcgg acggcatcgc ggacttgcca
     1201 cggttgaaca ggaacatggc gaaagagact ccaatcgtta ggtagttggg attctacgga
     1261 taggtacgca cgggccacgg cgatggatgc gtcgtgagaa gattctaggg ccgtgaagac
     1321 gggggagatg ctgcgagcca ccccgcgccg cttggtcacg cccatggtgc tggccgtgct
     1381 ggctggtgtc gtgctgggcg gcgcgctcgc cgtcccatcg ccggctgtcg ccgcgcagga
     1441 tctggcgggc atcgacgtct cgcactggca gggccagatc acctggagtg acgtcgcggc
     1501 cgacggcgtg agcttcgtga tcgccaaggc gaccgagggc cgcgtctacc aggacgacca
     1561 gtacgcgcgc aacaaggaac gggccgaggc caacggcatc gcgttcacgg cgtaccactt
     1621 cgccagcccc gacaagacgg ccaacgacgc gatcctcgag gcggacaact tcgtcgacac
     1681 cgcggacctg ggcccgggca acctgcggcc ggtgctcgac ctggagaaga acggcggcct
     1741 cggcgtcagg aagctgcggg cctgggtcca ggcgtggctg gcgcgggtcg aggaccgcct
     1801 gggggttaag ccgatcatct acacctcgcc gtcgttctgg cacgacaaga tgggcaacag
     1861 ccgctggttc gccaacaacg gctacgcgct gtggatcgcc cactggcatg tggccacgcc
     1921 gcgcgtcccg gccaggaact ggggcgggcg gggttggacc atctggcagt acgacgactg
     1981 cggctcggtc gccggcatcg acggctgtgt cgatctcgac cggctcaacg gcaaggacat
     2041 cgatgcgctc cggataccgg gcggttagcg ccgtggggcg ctcagatctc gtccgggttg
     2101 ggtgggccga ccggtgggtc gaagaagacc gcgacgcgtc ggtcgggcgc atcctcgtcg
     2161 ggcgcttgga ggatgaagcc gctgccgaag cgctggcgca ggccgcgccg caggtcgtgc
     2221 agcgtgcggt tggtcaaaca gatgtcggcc acttcggccg cgcgctgggc aacctccgtg
     2281 gtcggccaga agcgcgcata gacgtggatc gccacgaaac cgtcggcgag ccacgcgatc
     2341 cgaacagagt cgtcttcctg caccggctga ttgtgccgct tgtcgctgct cttgctgtcc
     2401 tgctgacccc ggcgtcggcc gcggccgccg gtgagccggc tggctacggg tatttccaca
     2461 cgtatgccga ggtgaaggcg cagatcgacg acacggtcgc ggcgcacccg ggcatcgtgc
     2521 agcgcttctc gatgggcacg agctacgagg gccgccccat ctggggcgtg aaggtcagcg
     2581 acaacgtggg ctccgatgaa ggcgagccgg aggtcttcgt cgtcgcccag ctccacgcca
     2641 acgaacgtgc gtcgggcgag atggcgctgt acctgatgga cctgttggtc gacggctacg
     2701 gcagcgactc gcgcatcacc gacatcgtga acagccgcga gatctggatc gtgcccgtgg
     2761 ccaacccgga tggtgccgag tacgacatgt ccgacggcgt cttccgccgc tggcgcaaga
     2821 accgccagcc cattccgggc tcgagctacg tgggcatcga cctgaaccgc cagttcggct
     2881 tcaagtggaa ctgctgcggc ggcggcagca acaacccgag ctcgcccaag taccgtggca
     2941 ccgagccgtg gcaggcaccg gaaacgcgcg cctatcgcga cttcatcctg agccgcgtcg
     3001 taggtgggca gcagcagctc gcgatcgcgc tgagcatcca tgcccagggt cggttggtcc
     3061 tctggccgta cgcctacacc aagaaggacc acccgcccac catgcgctac gacgactggc
     3121 gggcattcgt cgccctgggc cgggatgtcg ccgcgctgaa cggctacagg gcgcgccaga
     3181 caagcgacct gtacgtggtc gacggcgacg agggtgactg ggcgtaccac gcgcagggca
     3241 tcttcgcctt tgccttcgag ctcgccgcag gcagcccgga acgcatgtac ccgacgctcg
     3301 cccagctcca ggccgaccag gcccgcaacc ggcccgccgt gctcctgctc ctggaggcag
     3361 ccgactgtcc ttacgctgcc gtaggcgcgg ccttggcggc caagcactgc tcatgagcag
     3421 gccgctgcgg acgcgcgcct tcgcggtcct cctcgcgacg ctcgcttgcg ccgtgatgcc
     3481 cgggtcggcc attgccgcga acgcattccc ggccggtcac gagggctacc acaactacgc
     3541 cgagatgagc gcgttcctcg acgatgtcgc ggccgcccat ccaggcatcg ccgagaagtt
     3601 ctcgatgggc cgcagctacc agggccgcca ggtgtgggcg ctcaagatca gcgacaacgt
     3661 cggcaccgac gaaaacgagc cggaggtgct gttcacctcg cagatgcacg cgcgcgagtg
     3721 gctgtcgctc gaacaggacc tgcgcatcat cgagctgctc acctccaact accgcgccaa
     3781 ccccgggacg cagctcgagg gacgcgtcaa ggacatcgtc gatgggctcg agatctggat
     3841 cgtgccgatg ctcaatcccg atggggccga atacgacatc tccaacccca gcggcggctt
     3901 ccgcaaccag cgcaagaacc gccagccggt tggcgacggc gtccagcccg ggatcgacct
     3961 caaccgcaac ttcggcttca actgggcgtg ctgcggcggc tccagcggca agcccggctc
     4021 gttctactac cgcggcaggg cgccgtggca gggcgtcgaa acctccctgc tgcgcgactt
     4081 cgtgctcggt cgcatcgtcg gcggccggca gcagatccgc gcggctatcg actggcacgc
     4141 gttcaacgaa gagatcatgt ggcccttcgc ctacaccaag gccgatctgc cgcggccgat
     4201 ggccgccgac gatctacagg ccttcaaggc gatcgcccgg ggcatggccg atctcaacgg
     4261 ctacatgccc aaacagctga gcgacctgta cgtgttcgat ggcggcagca ccgactggct
     4321 gtacggcgac caacgcatct tctcgctcac gatcgaggtc tacccgctcg acaagtcgca
     4381 cccgggcttc tacccacccg acagcatcat cgagcgcgag acgacacgta acgacggtgc
     4441 cgtgctgtac ttcctcgaac aggtggcgtg tccgtatcgc gccgccggcc tggatgacac
     4501 gcactgcgga ccgctcgacg atgacttcga gaccggccgc ggctggaccg tggacccgct
     4561 cgacaccgac acggcgacgg gcggtgcctg gcagcgcgcg ataccggcca agagcaagac
     4621 gagcgccggc cagaagcagc gcgcctacgg gttcagcgcc cggtacgcgc tcgtcaccgg
     4681 ggcggcgcgc ggcgcgagtg ccaacgccaa cgacctcgat ggcctgacgt ccgtgcgttc
     4741 gccgcagttc gacctgggcg ggtcgagctc gagtggctgg cgtgtccagt tccgctacac
     4801 gttcgcgcac gcggcgaact ccagttcggc cgactacctg cgcctgctcg tcgatggcca
     4861 ggaggtatgg cgctccagcg gggcggcgag cgagcgcaaa gcgagctggg ggctggcgac
     4921 ggtgtcgctc gacgcgtatg ccggccagac cgtacggctc ctgttcgagg cgtgcgactg
     4981 tgccgccacg agcctggtcg aggcggtcgt ggacgatgtt cgcgtctaca aggtgccttg
     5041 atacaaccct gttagcgcct tggtatggac tcccgctggc cgaggggagt agcatcgaat
     5101 tccaacgagg tgcgagcgtg accaacgacc gcaagcgcca ccttacgtgg ccagatcgga
     5161 ggaccagcga gaccgcaacg tcgcgcgtcc tgccgccaac cgcggaccag tcggccggct
     5221 tcagcaacct cgcgacccag ccccgagcgg gcaacatcag cagcgatacg cgcgccggct
     5281 actgagcgtg atccggggcg agtgcgcgga acacatgtca cgttccgccc gcacggatca
     5341 ctggatccag aatcgaggac ccacacccgt tcccggggtg tgggtctttc gctaactacc
     5401 ttcctccgca gcttccgctg ctgcgcgcgg cactggtccg gccccgacgg accgtcggtg
     5461 ccgtcccgcc gcttgctccg gccccatcgc gtccctacag agtctcagtg accttggaga
     5521 tcgaccgcgg ccggtcgggg tcgaggccgc gcgcgatcgt gaggtggtac gcgtagagct
     5581 ggccgggcac gatctcgacg atcggcgaga gccagccggg cacgtctgac gggaggggca
     5641 gtccctcgtc gagcgcgagc atctccgcga cgtcggagat caccagcagg cgcgccccga
     5701 actcctggcg cagcctcgcg agcacctcga gctggccctc gtaggcaacg cctgacggcg
     5761 cgaccgcaag cacagcgaag cccggctcgg cgagggccag cgggccgtgc tcgaaatcgg
     5821 cagtggagta gggctggacc agcacgtgcg cgagctcctg gagcttgagc gaccactccc
     5881 gtgccgttgc gtacgcaaag ccgcggccga ggaccacgcc gtgcgttcgc agggcatggc
     5941 cggcgctgac cgagcgtgcc gcgtcttcag cggccagcgc cgctgcaacg agctcgggca
     6001 gccgcgcgag gtcggccgtc tcgtgggcgg acgccccgtc aagggccgtc gagagcagcg
     6061 cgaccgcaag cagctcggtg gtgtaggtct tggtcgcggc cgttgcgcgc tcgggcccgg
     6121 cgcgcaggtc gatcacctcg tctgccgccg ccgcgagggc cgacgccgcg tcgttggtca
     6181 tcgccaccgt cagcgcgccc tggccacgcg cctcgtcgag gacggccacg atgtcgggcg
     6241 agcggcccga ttgcgagatg ccgacgacga gcgcgtcgcg catgtcgggg cgcgccccgt
     6301 agagcgtgat cgtggatggc gcggcgagag cgacgggcag ggagttgcgg atcgcaaaca
     6361 ggtactgcgc atagagcgcg gcgttgtccg acgtgccacg agcggcgatg agcacgaagc
     6421 gcggccgccg ctcgcgcagc cgcgcggtca ggctcg
//
LOCUS       DATBRX010000157         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3968892, whole genome shotgun sequence.
ACCESSION   DATBRX010000157 DATBRX010000000
VERSION     DATBRX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3968892"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VIF08_06045"
     CDS             complement(<1..235)
                     /locus_tag="VIF08_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135584.1"
                     /translation="MPAYELPAELLIDTAVARRIIGEFIRGHLRQTGFERVILNLSGG
                     LDSALVAFLVAEAIGAEQLLCLLLPYRTSSPESR"
     gene            complement(239..1138)
                     /locus_tag="VIF08_06050"
     CDS             complement(239..1138)
                     /locus_tag="VIF08_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY8135585.1"
                     /translation="MSPSTRRNRLRIGLGQLAPSLGRVADNLVAHEQMIDKARSAAVD
                     LLVFPELGLTGYLLQDLNAEVAMATDDPRLVALAQRAGPMSTVVGFVERAPDEQLLIA
                     AALMEEGAIQHVYRKSYLPNYGLFDEQRFFGAGNRLRAVDSRLGVRLGLCICEDFWHL
                     PVPYLLALDGAQILVNISSSPGRDIAASTEGGLGTADSWGTIMRAYAQLTTTFVVFVN
                     RVGVDESVTFWGGSQVIDPAGDVAFAAPMLESGLFTCDIDLDDVRRERTAMTLLRDER
                     PEFVLRNLERLVREQAGVPQDAE"
     gene            complement(1190..2338)
                     /locus_tag="VIF08_06055"
     CDS             complement(1190..2338)
                     /locus_tag="VIF08_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8135586.1"
                     /translation="MIEGQNGAIVQVRGLRTYFPIKSRSLIPRVVGHVMAVDGIDFDI
                     AAGETLGLVGESGSGKTTAARSVLMLVKPTAGSVKIDGDELTRMSQSQLRPIRRKTSM
                     IFQDPYNALNPRHTVGSIISAPFEIQKVKPEGGVKRAVQDLMQRVGLNPEHYNRYPNE
                     FSGGQRQRIGIARAVALHPELVVCDEPVSALDVSIQAQILNLLHDLQKEFNIAYLFVA
                     HDLAVVRQVSHRIAVMYLGHIVESAPRKTIYAMPLHPYTRSLLSAVPVPDPVRQRQRT
                     RIVLQGDVPSPIDPPSGCVFHTRCWLYEKLGRPERCVDEVPPLRPFAEDQVVACHFAE
                     ELVGQPVGESAAETATEAAARVRKQDDDEAEAAAAADVAAHSQESPAP"
     gene            complement(2335..>2867)
                     /locus_tag="VIF08_06060"
     CDS             complement(2335..>2867)
                     /locus_tag="VIF08_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY8135587.1"
                     /translation="INSPELLIADEPTTALDVTVQAQILELIKLLQEEFGTTVIVITH
                     DLGIVADVADEVAVMYGARIMERASTQEIYEHPEMPYTLGLLSSIPRMDRDVTGRLEP
                     IRGNPPSAIRLPAGCVFEPRCPYSGLTGGLAQTTRPELLPTGSGTDHLVRCHLPPDKR
                     REIAAEVMGKLRSAKA"
BASE COUNT          471 a          959 c          973 g          464 t
ORIGIN      
        1 cacgcgactc gggcgacgac gtgcggtagg gcaggagcag gcacagcagc tgctccgcgc
       61 cgatcgcctc agccacgagg aacgccacca gggcagagtc gaggccgccg gacaggttca
      121 ggatgacccg ctcaaatccc gtctggcgca ggtggccgcg gatgaactcg ccgatgatcc
      181 gccgcgccac ggcggtatcg atgagcagct ccgcgggcaa ttcgtaggcc ggcacgccct
      241 attccgcgtc ctgcggcacg ccagcctgtt cgcgcaccag gcgctcgagg ttgcgcagca
      301 cgaactccgg ccgctcgtcg cgcaggagcg tcatggccgt tcgctcgcgg cgcacgtcgt
      361 caaggtcgat gtcacacgtg aagagaccgc tctcgagcat cggcgcggcg aaggccacgt
      421 cacccgccgg atcgatgacc tgcgagccgc cccagaatgt caccgattca tccacgccga
      481 cgcggttcac gaagaccacg aaggtcgtcg tcagctgtgc gtaggcgcgc atgattgtgc
      541 cccacgaatc ggctgtgccc agcccgcctt ccgtgctcgc cgcgatgtca cggccgggcg
      601 acgacgagat attgacgagg atctgcgccc cgtcgagcgc cagcagatag ggcaccggca
      661 ggtgccagaa gtcctcgcag atgcacaagc ccaggcgcac gcccaggcgc gagtcgacgg
      721 cgcgcagccg gttgccggca ccgaagaagc gctgctcgtc gaacagcccg tagttgggca
      781 ggtacgactt gcgatagaca tgctggatcg cgccctcttc catcagcgcg gcggcaatga
      841 gcagctgctc gtccggcgcg cgctcgacga agccgacaac cgtgctcatg gggccggcac
      901 gctgcgccaa cgcaaccagg cgcgggtcgt cagtcgccat cgccacctcg gcgttgagat
      961 cctggagcag gtagcccgtc aggcccagct cggggaacac cagcaggtcg accgcagcgg
     1021 atcgggcctt atcgatcatc tgctcgtgcg ccacgaggtt gtccgcgacg cggccaaggc
     1081 tgggcgcgag ctggcccagc ccaatgcgca ggcggttccg gcgtgtgctc gggctcatgg
     1141 cgccgatggt agcggcgggc gtggcccgga ctgatgcccg cagcgaacct taaggagcgg
     1201 ggctctcctg gctgtgcgcg gccacgtccg cggctgcggc ggcctccgcc tcgtcgtcgt
     1261 cctgcttcct gactcgcgcg gccgcctcag tcgcggtctc cgccgcgctc tcgcccacgg
     1321 gctggccgac cagctcctcg gcgaagtggc acgcgaccac ttgatcctca gcgaagggcc
     1381 gcagcggcgg aacttcgtcg acgcagcgct cgggccggcc gagcttctcg tacagccagc
     1441 agcgcgtgtg gaacacgcaa ccggagggcg gatcaatcgg gctgggcacg tcgccctgga
     1501 gaacgatgcg cgtgcgctgg cgctgccgca ccgggtccgg cacgggcacc gccgagagca
     1561 gcgagcgcgt gtatgggtgg agcggcatcg cgtagatcgt cttgcgcggt gcgctctcca
     1621 cgatgtggcc caggtacatg accgcgatgc ggtgcgacac ctgccgcacg actgccaggt
     1681 cgtgcgccac gaacaggtag gcgatgttga attccttctg caggtcgtgc agcaggttga
     1741 ggatctgtgc ctggatcgag acgtcgagcg cgctgaccgg ctcgtcgcac acgaccagct
     1801 ccgggtgaag agccacggcg cgggcgatgc cgatgcgctg acgctggccg ccgctgaact
     1861 cgttggggta gcggttgtag tgctcagggt tgagccccac gcgctgcatc aggtcctgta
     1921 ccgcgcgctt cacgccgccc tcgggcttga ccttctggat ctcgaacggc gccgagatga
     1981 tcgacccaac cgtgtggcgc ggattgagcg cgttgtatgg gtcctggaag atcatcgagg
     2041 tcttacgccg gatgggcctg agctggctct ggctcattcg ggtcaactcg tcgccgtcta
     2101 tcttgaccga gccggccgtg ggcttgacga gcatgaggac ggagcgcgct gcggtcgtct
     2161 ttcccgagcc gctctcgccc acgagaccca acgtctcgcc cgcggcaatg tcgaagtcga
     2221 tgccgtccac tgccataacg tggccgacaa cgcgcggaat caggctgcgc gacttgattg
     2281 ggaaatacgt gcgtaggccg cgcacctgaa cgattgcgcc gttctggccc tcaatcatgc
     2341 cttggcgctc cgcaacttgc ccatgacctc tgccgcaatc tcgcgccgct tatcgggcgg
     2401 cagatggcag cgcaccaggt ggtcggtccc cgaaccagtc ggcaagagct caggccgcgt
     2461 cgtctgggcc agcccgccgg tcaggccgga gtacggacag cgtggctcaa agacgcaccc
     2521 ggccggcagt cggatggcgg acggcggatt gcccctgatg ggctcgagtc gcccggtgac
     2581 atctctgtcc atgcgcggaa tcgacgatag gagtcccagg gtgtacggca tctcagggtg
     2641 ctcatagatc tcctgggttg aagcgcgctc catgattcga gcgccgtaca tgacggctac
     2701 ctcatcggcc acgtcggcaa cgatccccag gtcgtgggtg attacgatga cagtcgtgcc
     2761 gaactcctcc tggagcagct tgatcagctc caggatctga gcctgcaccg tcacgtcgag
     2821 ggccgtggtc ggctcgtcgg cgatgagcag ctccggggag ttgatga
//
LOCUS       DATBRX010000158         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1957563, whole genome shotgun sequence.
ACCESSION   DATBRX010000158 DATBRX010000000
VERSION     DATBRX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957563"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1293)
                     /locus_tag="VIF08_06065"
     CDS             complement(124..1293)
                     /locus_tag="VIF08_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135588.1"
                     /translation="MTPTGHDRTGTLKAIESWFVAEGIPHFVADHSPTRVVLRRAAPV
                     LVAYLAVTLLMTASFTWSFEVNVLAVLGAAALVLGGWILVNIVRGRRWLSLPDRFGYF
                     EVGAFLVIPALPPLIIGLQVSDAVIALIESAIFLAIVYVATSYGLIGAVRWAMRRARA
                     QLGSLGRLLTRALPLLMVFVAFTFLSSTTWQVMAAMSWQAIATVVLFFLVLSLAFLIG
                     RLVPEIRRMASSEREQLETVTLVKGTPAEQLADLAGEAAAPAVPLRWHEWVNVGTLIV
                     FSQGIQIVLVTFAVQLALVVFGLLLVPAPIQAEWIGRPVTPLYSAEVGQENIALTGEL
                     LVVSLILGAFSGLYFTVSALSDSAYRAEFFSDADRELNQIFAVRSVYHAARRPVI"
     gene            1347..>1865
                     /locus_tag="VIF08_06070"
     CDS             1347..>1865
                     /locus_tag="VIF08_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY8135589.1"
                     /translation="MPELPEVETIARELRPDVVGRTIKNAWFDWPNQIKHPAPTEFVA
                     AVKGREILSVERRAKWLVFALGPTKARGGPAVLAIQVKMTGQLDVVSPKAPRDRHVHA
                     IFSLDDGRELRLRDTRKFGRLAVYSPADAQRVLGTLGPEPLGEELTLAEFRRMLRGRR
                     GRLKTLLMNQAFL"
BASE COUNT          364 a          616 c          637 g          248 t
ORIGIN      
        1 gcgaggagct cgcggggatc gccccggtgg tgcgcgagga gatggagggc gcggtcaagc
       61 tcgacgtgcc gctgaccgtg gagatgaaga gcggcaccga ctgggagtcg ctgacgccgc
      121 tgcctagatg accgggcgcc gcgcggcgtg atagacagaa cggacggcga agatctggtt
      181 gagctcgcga tcggcgtcgc tgaagaactc agcccgatag gcgctatccg aaagcgctga
      241 gaccgtgaag tacaggcctg agaaagcgcc caggatgagc gacaccacga gcagctcgcc
      301 ggtcaaggcg atgttctcct ggcccacctc ggcgctgtag agcggcgtga ccggccgccc
      361 gatccactcg gcctggatgg gcgccggaac gagcaatagg ccgaacacca ccaatgccag
      421 ctgaacggca aacgttacga gcacgatctg gatgccctgg ctgaacacga tcagcgtgcc
      481 cacgttcacc cactcgtgcc agcgcagggg gactgccggc gcggcggctt cgccggccag
      541 atcggccagc tgctcggcgg gagtgccctt gacgagcgtc accgtttcca gttgctcacg
      601 ctcggaggac gccatacgtc gaatctccgg caccagccgg ccgatcagga aggcgagcga
      661 cagaaccagg aagaacagca cgacagtggc gatcgcctgc cagctcatcg cggccatgac
      721 ctgccaggtc gtactcgaca ggaaggtgaa ggcgacgaac accatcaaca gcggcagggc
      781 gcgcgtcagg agacggccga ggctgcccag ctgcgcacgc gcacgacgca tcgcccagcg
      841 caccgcaccg atgagcccgt aggaggtggc gacgtagacg atcgccagga agatggcact
      901 ctcgatgaga gcgatcacgg cgtcgctcac ctggaggccg attatcagcg gcggcagcgc
      961 ggggatgacc aggaaggccc cgacctcgaa gtagccgaat cggtcgggca gcgacaacca
     1021 gcgtcggccg cgaacgatgt tgaccaagat ccaaccgccc agtacgagcg ccgcggcgcc
     1081 caaaacggcc agcacgttga cctcgaatga ccaggtgaag ctggccgtca tgagcagcgt
     1141 caccgcgagg taagccacca gcaccggcgc cgcccggcgc agtacgactc gcgtcggtga
     1201 gtgatcggca acgaagtgcg gtattccctc cgcgacgaac cacgactcaa tcgccttgag
     1261 cgtgcctgtc cgatcgtgcc ccgttggtgt cacagtggcc acggtacgtt cccggacggc
     1321 gaaagggcaa cctagactca cgacacatgc ctgagctgcc cgaggtcgag acgatcgcgc
     1381 gcgagctgcg cccggacgtc gtcggccgca cgatcaagaa cgcgtggttc gactggccca
     1441 accagatcaa gcaccccgcg ccgacggagt tcgtcgctgc cgtcaagggg cgcgagatcc
     1501 tgtccgtcga gcggcgcgcc aagtggctcg tcttcgcgct gggtccaacg aaggcgcgcg
     1561 gcggaccggc ggtgctggcg atccaggtca agatgaccgg gcagctcgac gtcgtgtcgc
     1621 ccaaggcgcc gcgcgacagg catgtccacg ccatcttctc gctcgacgat ggtcgcgagc
     1681 tgcgcctgcg cgacacgcgc aagttcgggc gactcgcggt ctatagcccg gccgacgccc
     1741 agcgggtgct gggcacgctg ggtcctgagc cgctgggcga ggagctgacg ctcgcggagt
     1801 tccggcgcat gctgcgcggc cgccgcggtc ggctcaagac gctgctcatg aaccaggcat
     1861 tcctc
//
LOCUS       DATBRX010000159         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2517658, whole genome shotgun sequence.
ACCESSION   DATBRX010000159 DATBRX010000000
VERSION     DATBRX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517658"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VIF08_06075"
     CDS             <1..530
                     /locus_tag="VIF08_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY8135590.1"
                     /translation="IASTQVLQRATVREILMGAQDNLTNVLAVVLGVTIGSGRADFVA
                     LAGLSAGIAESVSMGGVLYNATRAEQQMAAAAGRTTDKADPSDLSPVLSGLVTFGAAL
                     VAGLVPLAPFALLPVGTAIVVSMGLSLVGLFALGSVTGHVAGGTWLTEGLRLIAVAGI
                     AALAAAVVGATLQVA"
     gene            568..870
                     /gene="gatC"
                     /locus_tag="VIF08_06080"
     CDS             568..870
                     /gene="gatC"
                     /locus_tag="VIF08_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY8135591.1"
                     /translation="MATLSRKDVEHVAHLARLGLTEDELARLEGQLNHILDQYAVLAE
                     LDTEHIAPTAQTIELENILRDDVVAPSLDADEVLALAPERSGQYFLVPPILGEHGN"
     gene            893..2482
                     /gene="gatA"
                     /locus_tag="VIF08_06085"
     CDS             893..2482
                     /gene="gatA"
                     /locus_tag="VIF08_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY8135592.1"
                     /translation="MTSDLTRLRAHEMAAGLRRGDFSAHDLAAAHLDRIAATDGPLHA
                     WVWWDRAAALGQADAADDRLARAREAGADALAALPALLGVPVGLKDLVLTRGDRATAG
                     SRILDGYISPYDAHIAENLRAAGAVLPGKTNMDEFAMGSSTEFSAWGPTANPWDLDRV
                     PGGSSGGSAAAVAAYHVPLSIGTDTGGSIRQPAALTGIVGLKPTYGRVSRYGIVAFAS
                     SLDQVGPFGRDVRDAAMLMSVIAGRDERDSTSAPVPVPDYAAHLPESDDEAAASLRGV
                     RLGLPKQYFVKGMEPGVEARIREAVAALEAAGAEVVDIDLPHTDYGLATYYIVAPAEA
                     SANLARYDGVRYGRSVRLAGGDYIADYLETRGTGFGAEVKRRIMLGTYALSAGYYDAY
                     YLKAQKVRTLIKADFDAAFEKVDALVAPTSPTVAFKFGTRLQDPVAMYLSDACTLPAN
                     MAGVCGLSVPCGLSDGLPVGLQLIGRPWDEDRLFRLGRAYEAITATAEWRAMQPTDLA
                     RLADPATPTAADRAAVVTSAA"
     gene            complement(2471..>2712)
                     /locus_tag="VIF08_06090"
     CDS             complement(2471..>2712)
                     /locus_tag="VIF08_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135593.1"
                     /translation="DQQVVAAVEGLGDGAWWRHHVELPNVHIDTDDAVLTVITEPVRF
                     SIAFIQIPNPVGVDSAEWLANLTELANDVLVALGR"
BASE COUNT          402 a          967 c          931 g          412 t
ORIGIN      
        1 cgattgcctc gacccaggtc ctccagcgcg caacggtgcg cgagatcctc atgggcgccc
       61 aggacaacct gaccaacgtg ctcgcggtgg ttcttggcgt gaccatcggc tccggaaggg
      121 ccgacttcgt cgcgctggcc gggctgtcag ccggcatcgc cgagtcggtc tcgatgggcg
      181 gcgtgctcta caacgcgacg cgtgcggaac agcagatggc agcggcggcc ggccgcacga
      241 cggacaaggc cgacccgtcc gatctgtcgc ctgtcctttc cggcctggtc acctttggcg
      301 cggcgctcgt cgccggcctc gtgccgcttg cgccgttcgc gctgctgccc gtgggcacgg
      361 cgatcgtcgt ctcgatgggc ctgtcgctcg tcggcctctt cgccctcggc tcagtcaccg
      421 gccacgtcgc cggcgggaca tggctcaccg aagggttgcg cctcattgcc gtcgcgggca
      481 ttgcggctct ggccgcggcc gtcgtcggcg ctacgctgca ggtcgcctga accggttggg
      541 agcctgccgc cgctaccatt cggcgcgatg gcgaccctgt cacgcaagga cgtcgagcac
      601 gtcgcgcacc tcgcccgcct cgggctgacc gaggatgagc tggcgcggct cgagggccag
      661 ctcaaccaca tcctcgacca gtacgcggta ttggccgagc tcgacaccga gcacatcgcg
      721 ccgaccgccc agacgatcga gctggagaac atcctgcgcg acgacgtggt ggcgcccagc
      781 cttgacgcgg acgaggtgct ggcgcttgcc ccggagcgtt ccggccagta cttcctcgtg
      841 ccgcccatcc tgggcgagca cggcaactga ccaaccggcc gattcacccg agatgacctc
      901 cgatctgacc cgcctgcgcg cccacgagat ggccgccggc ttgcgccgcg gcgatttcag
      961 cgcgcacgac ctggcggcag cgcacctcga tcgcatcgcg gcgaccgacg ggccgctcca
     1021 cgcctgggtg tggtgggacc gcgccgccgc gctcggccag gccgacgccg ccgacgatcg
     1081 cctggcgcgc gcccgcgaag caggtgcaga cgcgctcgca gcgctgcccg ccctgctggg
     1141 cgtgccagtc gggctgaagg acctggtgct cacccggggc gacagggcaa ccgcgggcag
     1201 tcggatcctg gacggctaca tcagcccgta cgacgcgcac atcgcagaga acctgagggc
     1261 cgccggcgcc gtgttgcccg gcaagaccaa catggacgag ttcgcgatgg gctcatcgac
     1321 cgagttcagc gcgtggggcc caacggccaa tccgtgggac ctcgatcgtg tgccgggcgg
     1381 ttcgagcggc ggctccgcgg cggcggtggc cgcgtatcac gtgccgctgt ccattggcac
     1441 cgacaccggc ggctccatcc gccagccagc ggcgctgaca ggcatagtcg ggttgaagcc
     1501 gacctacggc cgcgtatcgc gctacggcat cgtcgccttt gcctcgtcgc tcgaccaggt
     1561 cgggcccttc ggccgcgacg tgcgtgacgc ggccatgctg atgagcgtca tcgcgggccg
     1621 cgacgagcgt gattccacgt ccgcgccggt gccggtgccc gactacgccg cgcatctgcc
     1681 ggagagcgac gatgaggccg ccgcgagcct gcgcggcgtc cgcctggggc tgccaaagca
     1741 gtatttcgtc aagggcatgg agcccggtgt ggaggcgcgc atccgcgagg cggtcgcggc
     1801 gctggaggca gcgggcgcgg aagtggtcga catcgacctg ccgcacaccg actacggcct
     1861 cgccacgtac tacatcgtcg cgcccgccga ggcgtcggcg aacctggcgc gctacgacgg
     1921 cgtgcgctat ggccgctcgg tgcgcctggc gggcggcgac tacatcgcgg attacctcga
     1981 aacgcgcggc acggggttcg gcgcggaggt caagcgccgg atcatgctcg gcacgtacgc
     2041 gctctcggcc ggctactacg acgcgtacta cctcaaggcg cagaaagtgc gcacgcttat
     2101 caaggccgac ttcgatgccg cgttcgagaa ggtcgacgcg ctcgtcgccc cgaccagccc
     2161 gacggtcgcc ttcaagttcg gcacgcgcct ccaggacccg gtcgcgatgt acctgtccga
     2221 tgcctgcacg ctgcccgcca acatggccgg cgtgtgcgga ttgtcggtgc cgtgcgggct
     2281 gtcggatgga ttgccagttg gactgcagct gatcggccgg ccatgggacg aggatcggct
     2341 cttccggctg ggccgggcat atgaggcgat caccgccaca gccgagtggc gtgccatgca
     2401 acccacggac ctggcccgcc tcgcggatcc cgcgacgccc accgcggcag accgcgctgc
     2461 agtcgtgacg tcagcggcct aacgcgacaa gcacgtcgtt cgccagctcc gtcaggttgg
     2521 ccagccactc ggccgagtcg acacccacgg gattaggaat ctgaatgaaa gcgatggaga
     2581 atcggaccgg ctcggtgatc acggtgagca ccgcgtcgtc agtgtcaatg tggacattgg
     2641 gaagctcgac gtgatgcctc caccatgcgc cgtcgcccaa tccctcgacg gcagctacga
     2701 cctgctggtc ac
//
LOCUS       DATBRX010000160         4866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3635452, whole genome shotgun sequence.
ACCESSION   DATBRX010000160 DATBRX010000000
VERSION     DATBRX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635452"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VIF08_06095"
     CDS             complement(<1..1131)
                     /locus_tag="VIF08_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8135594.1"
                     /translation="MWNGLGSIVLPKRAEALFGPAWAGLGLDLITVLGVIAALLVQPT
                     VGAISDFTASRWGRRKPYIVVGSALSVAFLWAVAAADSPFAIIVALVLLQIASNIAQG
                     PFQGYVPDLVPSKQVGLASGLMGVMTIVGGVAGVAICLVGYQQLQTGMADAQVRALLF
                     WPTIALGVIQLATMVVMALTVDEGRGAVPREGRSWLQLARSAWDTDILRERSFVWLLV
                     SRLCFLATIGVISAFAVFLLERTFDLPPSEAQSYLLVIGVIIAGSTIVVAIPAARLSD
                     RLGRKRVINIAFVLGAIGVAGVAAAPTVPLAMVALVPLGFSGGAFLVVDWALMTDIIP
                     KARTGRYMGISNVATASAGPIGLALGGVILLLVTIAGKSAPAA"
     gene            complement(1300..2694)
                     /locus_tag="VIF08_06100"
     CDS             complement(1300..2694)
                     /locus_tag="VIF08_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8135595.1"
                     /translation="MAATTPATDVTEGAAKPARLPLAHLLNLSVYWAGLNTVWAGLGF
                     VVFPTRLTETYGPAYAPSYVLLFETIPVLFAVLVQPTVATISDYTISRWGRRKPYIFI
                     GAILDVLFLWGFAASNELVVMFAFIMLLQFSANFAQGPFQGYVPDLVPKEQVGLASGL
                     MGVMIVIGQVTGAGIGALGLIALKASPFEQGTAAAGAFAQEAFFLPTIGLAVVEVVTM
                     IPLVLFIREGRSAPSRQGRSWTQIALSAWGTDILKERNYVWLLVSRLFFLMAPTLPLS
                     LGVFYLRQSLGSSDELAGIQIFIIAGVVGVTTGLATLPAARLSDTYGRKTMIYAGIAI
                     GILGNIGVALAPGFEIALGALILVGISSGSFLAVDWALMTDIIPKSTTGRYMGISAVA
                     TGLAGPLGRFIANPLLTLLIIVGLTAGMDPAVDAPQSAFYATGPRLVILLGVVFFVIS
                     AWALRHVDERRRED"
     gene            2748..4058
                     /locus_tag="VIF08_06105"
     CDS             2748..4058
                     /locus_tag="VIF08_06105"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8135596.1"
                     /translation="MPDLPTIQFSQRTLPNGLRVIVAPDHLAPVVAVNLWYVVGSRHE
                     TEGRTGFAHLYEHFMFQGSRHVTKAEHFSIIQSAGGVNNATTYFDRTNYFETLPSHQL
                     DLALWLEADRMATLLDALDQENLDNQREVVKNEKRQTYDNRPYGSFYEKLMAAVFPPE
                     HPYHHTPIGSMEDLDAATVEDVVDFFKTWYAPNNCVLSIVGDVDEEAAHAAAARYFGP
                     IPANPAIVQPPQDKIAPVIGVEQREVVPDAVPLTRIHFGFRCPPYGSAEFDALEVASQ
                     ILAGGRGSRMYRSLVREKKIAQDVTVFGLPLVDGASFFGGWVTVRPDSDEEACEDAFL
                     AELQRLVDEPVTDDELARSRALIEAAELGALSRVEEVADRLSMFAALFNRPELINEQL
                     GRYLAVTAEQIQAVAAKVFRADNRVVLSYVPNEAGTDDEEGAAA"
     gene            4055..>4866
                     /locus_tag="VIF08_06110"
     CDS             4055..>4866
                     /locus_tag="VIF08_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8135597.1"
                     /translation="MTLEDPAEKGFITDSPEEADAGDTTLTDDGLPQAPTSAAVADLS
                     ALDVRPTAGAPRDYHFPRFERFRLANGLTVLHAHVAGRALIAANLLIPGGGWTEPPEQ
                     GGVTVLTSRAMPEGTQRLDADEFIEQSERLGAEIHAEATWEALTAGMEVPRSHFGAAL
                     ALLAEMVFEPAFPEQEVERLREERMNDLLQAWSDPRRRAERVFPETIFGPASPYRRPL
                     AGVQGTVRGLTRADVQARHAALLNPATATLVVAGDLGSQRLDQIAEEHLGGRS"
BASE COUNT          849 a         1664 c         1684 g          669 t
ORIGIN      
        1 cgcggcgggc gccgactttc cggcgatggt cacgagcaat aggatcacgc cgcccagggc
       61 aagcccgatc gggccggcag aggccgtggc gacgttggag atgcccatgt atcggccggt
      121 gcgggccttg gggatgatgt cagtcatcag cgcccaatcg accacgagga acgcgccgcc
      181 cgagaagccg agtgggacca atgcgaccat cgccagcggg accgtcggcg ccgcagccac
      241 gccagccacc ccgatcgcgc ccagcacgaa cgcgatgttg atcacgcgct tgcggccgag
      301 tcggtcggac aggcgcgcgg ccgggatggc gaccacgatc gtcgagccgg cgatgatcac
      361 tccgatgacc agcaggtagc tctgcgcctc gctcggtggc aggtcgaagg tgcgctcgag
      421 caggaacacc gcgaaggcgc tgatgacgcc gatcgtcgcg aggaagcaca gccgcgacac
      481 cagcagccag acgaacgagc gctcgcgcag gatgtcggtg tcccatgccg atcgggcgag
      541 ttgcagccac gagcggccct cacgcggcac ggcgccgcgg ccctcatcca cggtcagcgc
      601 catgaccacc atcgtcgcca gctggatgac gcccagagcg atcgtcggcc agaacagcag
      661 cgcgcggacc tgcgcgtcgg ccatgccggt ctgcagctgc tgatagccaa cgaggcagat
      721 cgccacgccg gccacgccgc cgacgatcgt cattaccccc atcaggccgc tcgccaggcc
      781 gacctgcttc gatggcacga ggtcgggcac gtaaccctgg aacgggccct gcgcgatgtt
      841 cgacgcaatc tggagcaaca ccagggcgac gatgatcgcg aatggcgaat cggccgccgc
      901 gaccgcccac aggaaggcga cgctgagcgc gctgccgacg acgatgtacg gcttgcgccg
      961 gccccagcgt gaggcggtga agtcggagat tgcaccgacc gtcggctgga cgagcagcgc
     1021 cgcaataacg ccgagcaccg tgatcagatc gagccccagc ccggcccacg ccgggccgaa
     1081 cagcgcctcg gcgcgcttgg gcagcacgat cgagccaagg ccgttccaca tggcggtgat
     1141 gccgagccag tagatggata gcgtcagcag ctgtccggtc gggaggcttt tcgtgggtcg
     1201 cccgagtacg ggtgaatcgg acgtctgggg cgttacgggc gcaaaggggt ctagggtcgt
     1261 cggttcgatt ggcggagttt aggcggtggg tcggcggcct cagtcctcgc ggcggcgctc
     1321 gtccacgtgg cgcagtgccc acgccgagat gacgaagaac acgacgccga gcaggatcac
     1381 cagccgcggc ccggtcgcgt agaacgcgct ctgtggggca tcgaccgcgg gatccattcc
     1441 agccgtcagg ccgacgatga tcagcagcgt cagcagcgga ttggcgatga agcgacccag
     1501 cggtccggcg aggccggtcg ccacggccga gatgcccatg tagcgaccgg tcgttgactt
     1561 ggggatgatg tcggtcatga gcgcccaatc gaccgccagg aacgagcccg acgagatgcc
     1621 cacgagtatc agcgcgccca gcgcgatctc gaagccgggc gccagcgcga caccgatgtt
     1681 gcccaggatg ccgatggcga tgccggcgta gatcatggtc ttgcggccgt acgtgtccga
     1741 cagccgcgcg gcgggcagtg tcgcgagacc cgtggtcacg ccgacgaccc cggcaatgat
     1801 gaagatctgg atcccggcga gctcgtcgga tgagccgagc gactggcgca ggtagaagac
     1861 gcccagcgac agcggcagcg tgggcgccat caggaagaac aggcgcgaca ccaacagcca
     1921 gacgtagttg cgctccttca ggatgtccgt gccccaggcg ctcagcgcga tctgtgtcca
     1981 cgagcggccc tggcgcgagg gggcggagcg tccttcgcgg atgaacagga ccaaggggat
     2041 catcgtcacg acctcgacca ccgcgaggcc gatcgtcggc aggaagaacg cttcctgggc
     2101 gaaagcgccc gcggccgccg tgccctgctc gaacgggctg gctttcaggg cgatgaggcc
     2161 cagcgcgcca atgcccgcgc cggtcacctg gccgatgacg atcatcacgc ccatcagccc
     2221 gctcgcgagc cccacctgct ccttgggcac cagatccggc acgtagccct ggaacggccc
     2281 ctgggcgaag ttggcgctga actgcagcag catgatgaag gcgaacatca ccacgagttc
     2341 gttcgatgcc gcgaagcccc acaggaacag cacgtcgagg atcgcgccga tgaagatgta
     2401 tggcttgcgc cggccccagc ggctgatggt gtagtcggag atcgtggcga cggttggctg
     2461 gacgagcacc gcgaagagca cagggatcgt ctcgaacagc aggacgtagg acggtgcgta
     2521 ggccggcccg taggtctcgg tgagacgggt ggggaacacg acgaagccga gccccgccca
     2581 gacggtgttg agaccggccc agtagaccga caggttgagc aggtgcgcca gcggcaggcg
     2641 cgcaggcttg gccgccccct ccgtcacgtc cgttgccggg gtcgtggccg ccacctcgcg
     2701 acgatgatcg cgccccggtc aagggtcgcg gtgcgacaat ccgcgccgtg cccgacctac
     2761 cgaccatcca attcagccag cggacactgc ccaacggcct gcgcgtgatc gtggcgccgg
     2821 accacctggc gcccgtcgtc gcggtcaacc tctggtatgt cgttgggtcg cgccatgaga
     2881 cagagggccg gaccggcttt gcgcacctgt acgagcactt catgttccag ggctcgcgcc
     2941 acgtcaccaa ggccgagcac ttctcaatca tccaatccgc cggcggcgtg aacaacgcca
     3001 cgacctattt cgaccgcacc aactacttcg agacgctgcc ctcgcaccag ctcgacctcg
     3061 ccctgtggct ggaggccgac cgcatggcga cgctccttga cgcgctcgac caggagaacc
     3121 tcgacaacca gcgcgaggtg gtcaagaacg agaagcgcca gacgtacgac aaccggcctt
     3181 acggctcgtt ctacgagaag ctcatggccg ccgtcttccc gcctgagcat ccgtaccacc
     3241 acacgccgat cggctcgatg gaggacctcg acgcggccac ggtcgaggat gtcgtggact
     3301 tcttcaagac ctggtacgcg cccaacaact gcgtgctgtc gatcgtgggc gacgtcgatg
     3361 aggaagcggc gcatgccgct gccgcgcgct acttcgggcc catccccgcg aatccggcga
     3421 tcgtccagcc gccgcaggac aagatcgcgc cggtcatcgg cgtcgagcag cgcgaggtgg
     3481 tgccggacgc ggtgccgctg acgcgcatcc acttcggctt tcgctgcccg ccgtacggca
     3541 gcgccgagtt cgacgcgctg gaggtcgcga gccagatcct tgccggcggc cgcggctcgc
     3601 gcatgtaccg ctcgctggtg cgcgagaaga agatcgccca ggacgtgacg gtcttcgggc
     3661 tgccgcttgt cgatggcgcg tcgttcttcg gcggctgggt cacggttcgc ccggacagcg
     3721 acgaggaggc atgcgaggac gccttcctgg cggagctgca gcgccttgtc gacgagcctg
     3781 tgaccgacga cgagctcgcc cgttcgcggg cattgatcga ggccgccgag ctgggcgcgc
     3841 tcagccgcgt cgaggaggtc gccgatcggc tgtcgatgtt cgcggccctg ttcaaccgcc
     3901 ccgagctcat caacgagcag ctcgggcgct acctcgcggt gaccgcagag cagatccagg
     3961 cggttgcggc gaaggtcttc cgggcagaca accgcgtcgt tctgagctac gtgcccaacg
     4021 aggcgggcac ggacgatgag gaaggggcgg ccgcgtgacg ctggaggatc cggccgagaa
     4081 gggcttcatc accgattcac cggaggaagc cgacgcaggg gataccaccc tcaccgacga
     4141 tgggctgccc caggcgccga cgagcgcggc tgtggccgac ctctcggcgc tcgatgtccg
     4201 acccaccgcc ggtgcgccgc gcgactacca cttcccacgc ttcgaacgct tccggctggc
     4261 caacggcctg accgtgctcc atgcccacgt cgcgggccgc gccctgatcg ccgcgaacct
     4321 gctgataccg ggcggcggct ggacggagcc gccagagcag ggtggcgtga ccgtgctgac
     4381 cagccgcgcg atgccggaag ggacgcagcg cctcgatgcc gacgagttca tcgagcagag
     4441 cgagcggctg ggcgcggaaa tccacgccga agcgacgtgg gaggcgctga ccgcgggcat
     4501 ggaagtgccg cgctcgcact tcggtgcggc gctggcgctg ctcgcggaga tggtcttcga
     4561 gccggcattc cccgagcaag aggtcgagcg gctgcgcgag gagcgcatga acgacctgct
     4621 ccaggcgtgg tccgatccgc ggcgccgcgc cgagcgcgtc ttcccggaga cgatcttcgg
     4681 ccccgcctcg ccctaccggc ggccgctcgc cggcgtccag ggcaccgtcc gcgggctcac
     4741 ccgcgccgac gtccaggcgc gccacgcggc gctgctcaat cccgccacgg cgacgctggt
     4801 cgtggccggc gacctgggca gccagcgcct cgatcagatc gcggaagagc accttggcgg
     4861 gcgctc
//
LOCUS       DATBRX010000161         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_60109, whole genome shotgun sequence.
ACCESSION   DATBRX010000161 DATBRX010000000
VERSION     DATBRX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60109"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VIF08_06115"
     CDS             <1..268
                     /locus_tag="VIF08_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY8135598.1"
                     /translation="DPKLDDPALVEAFRRGCRYVGALGSRTTQADRRERLRAMGVRDA
                     DVARLHGPIGLDLGGRAPAETALAILAEIVAERYGASAEPKSGR"
     gene            complement(298..945)
                     /gene="eda"
                     /locus_tag="VIF08_06120"
     CDS             complement(298..945)
                     /gene="eda"
                     /locus_tag="VIF08_06120"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY8135599.1"
                     /translation="MDPDTFAGRLAELRVVPVVTIDDAADAAALAGALADGGLPIVEI
                     TFRTAAAAEAIAAIRAARPDVLVGAGTVLDAATVDRALEAGAHFIVAPGFSPATVAYA
                     QAHGTPVVPGVVTPSEIEAAMSLGLRVLKFFPAEASGGPAYLSALRATYPMVRFVPTG
                     GINVGSLASYLALPNVIAAGGTWIATADAIRDQRWDEISRLAAEAVAMSSTTRES"
     gene            complement(957..1553)
                     /locus_tag="VIF08_06125"
     CDS             complement(957..1553)
                     /locus_tag="VIF08_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135600.1"
                     /translation="MRSVVTGLILVALVASVAACDAPARTPRPSPSLIILDFSPRPSG
                     SATASLSPGASQGGWPLGWDVSFCAMFVQAVDAQQLLVDVERALDEGANRDARLLADE
                     LIQSAGGAVDAIATLPEWADATATVVAIAGLMDLGERAGTEYHAWFAKDKQAALRRAR
                     DLRLENGIQVPAANDGLADLANAGLTCQDVPLVLEAPG"
     gene            complement(1594..>2158)
                     /locus_tag="VIF08_06130"
     CDS             complement(1594..>2158)
                     /locus_tag="VIF08_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135601.1"
                     /translation="GIGMVALALAVLAGAFVLVNRPPGPTPTRQVGPTASPPPGSPSA
                     LPSTQPTSDLPTPLPTPIATPFVPIVQVGPGFVTFGTEFDAGGNIADPRALFVPSDRI
                     SWSGYLSEPTDSADLTVRLYKLDDLVPGGLRLLSEGEARTRVGDRLHYTRSDFNPNRA
                     LEGPGVYLVRYLRTDIVLAEGYFEMGG"
BASE COUNT          338 a          740 c          773 g          307 t
ORIGIN      
        1 cgatcccaag ctcgacgatc cggcgctggt cgaggcgttc cggcgcggct gccgctacgt
       61 gggcgcgctg ggctcgcgca cgacccaggc ggaccggcgc gagcggctgc gcgccatggg
      121 cgtccgtgac gcggacgtgg cccggctcca cggcccgatt gggctcgacc tgggcggtcg
      181 cgccccggcc gagacagcgc tcgcgatcct cgccgagatc gtcgccgagc gctacggcgc
      241 gtctgccgaa cccaagtctg gacgctagat acctagatcg gccatctgcc cagctgatta
      301 gctctcgcgt gtcgtcgacg acattgcgac cgcttcggcg gcgaggcggc tgatctcgtc
      361 ccagcgctga tcgcggatcg catcggccgt cgcgatccac gtcccgcccg cggcaatgac
      421 attgggcagc gccaggtacg aggcgagact gcccacgttg ataccgccgg tgggcacgaa
      481 gcggaccatc gggtaggtcg cccggagggc cgataggtag gccgggccgc cggacgcttc
      541 tgccgggaag aacttcagca cgcgcaaccc gagcgacatg gccgcctcga tctcgctggg
      601 cgtcaccacc cctggaacca ccggtgtgcc gtgtgcctgg gcgtacgcca cggtcgcggg
      661 actgaagcct ggcgcgacga tgaaatgcgc acccgcctcg agggcgcgat cgaccgtcgc
      721 cgcatcgagc acggtaccgg cgccgaccag gacatcgggc ctggccgccc gtatggcggc
      781 aattgcctcg gcggcggccg cggttcgaaa ggtgatctcc acgatcggca ggccgccgtc
      841 ggccagcgct ccggccagcg cggcagcgtc ggccgcgtcg tcgatggtga cgaccggcac
      901 gacgcgcagc tccgcgaggc gccccgcgaa ggtatcgggg tccactcctg ctccgactac
      961 ccgggcgctt cgagaacgag cggcacgtcc tggcaggtga gaccggcatt ggccaggtcg
     1021 gcgaggccgt cgttggcggc aggaacctgg atcccgttct caaggcgcag atcgcgcgcg
     1081 cggcgcaatg ccgcctgctt gtctttggca aaccaggcgt ggtactcggt tcccgcgcgc
     1141 tcgcccaggt ccatcaggcc ggcgatggcg acgacggtgg ccgtggcgtc ggcccactcg
     1201 ggcagcgtgg caatcgcatc cacggcaccg cccgcgctct ggatcagctc gtcggcgagg
     1261 agccgcgcat cgcggttggc gccttcgtca agcgcccgtt cgacgtcaac cagcagctgc
     1321 tgggcatcca ccgcctgcac gaacatcgcg cagaagctga cgtcccagcc gagcggccag
     1381 ccaccttgcg acgcgccagg tgatagcgac gcggtggccg agccacttgg gcgaggcgag
     1441 aagtcgagga tgatcagtga cgggctgggg cgcggggtgc gcgccggcgc atcacacgcc
     1501 gccacgctcg cgacgagcgc gaccaggatc aggccggtga cgacggagcg catgaccgtc
     1561 ggtcaggata acgggcgccg gttcgatctc cggctatccg cccatttcga agtagccctc
     1621 ggccaggacg atgtccgtcc tcaggtagcg cacgagatag acgcccggcc cctccagcgc
     1681 acggttcggg ttgaagtcgg agcgcgtgta gtgcaggcga tccccgaccc tggtcctcgc
     1741 ttcgccctcg ctcaggagac gcaaaccgcc cgggaccaag tcatcgagct tgtacaggcg
     1801 cactgtcagg tcggcagagt cggtcggttc gctcaggtag ccgctccagg agatccgatc
     1861 gctgggcaca aacagcgctc gcggatcggc gatgttgcca cctgcatcga actctgtgcc
     1921 gaaggtcacg aagccggggc cgacctggac gatgggcacg aagggcgtcg caatgggggt
     1981 tggcagcgga gtcggcaggt ccgacgttgg ctgcgttgat ggcagcgcgc tgggcgaacc
     2041 tggtggcgga ctggcggtag gtccgacctg gcgcgtgggt gtcggcccgg gcgggcggtt
     2101 gaccagcaca aacgcgccgg cgagcacggc cagggcaagt gccaccatgc cgatgccc
//
LOCUS       DATBRX010000162         7889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2294078, whole genome shotgun sequence.
ACCESSION   DATBRX010000162 DATBRX010000000
VERSION     DATBRX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2294078"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VIF08_06135"
     CDS             complement(<1..906)
                     /locus_tag="VIF08_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8135602.1"
                     /translation="MYAVILAGGGGTRLWPLSDPRHPKPFLPLLPDGASLLQRTVTRL
                     MSGDELNLTLDDIHVVTDRRYASLVSDQTDVGVIAEPVGRNTAAAVALAATSIERDPD
                     ELMVVLPADHLVQRDDVFRAVLADAQRELAQGAFGIESPIVTLGATPTGPSTHYGYLV
                     PDPARQQLNRLHAYALSRFVEKPDAAKAAELLSIPDVCWNAGIFMARRRAWLAALEKH
                     TRLVGPLANTRDSESRLEAAYAEIEPISIDYAVMEPAAAEGQVVMGAMDVGWSDVGTW
                     TALLDALVGGYTAPARVVPAGDAAHL"
     gene            965..3049
                     /locus_tag="VIF08_06140"
     CDS             965..3049
                     /locus_tag="VIF08_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8135603.1"
                     /translation="MLRRIPPPVLVAFILAAACTNPTPSPTPAPSGSANPTPTTSAVP
                     TTTAGPTTTAGPTPGASGTGPQVVTGSVSYSNAFFTAGVSQPLIILEDQGGFVTRDRN
                     FAISEPSQVIGAITSNFFTSPFTYTLTLPAQPNGSVHDVDHDGQQDQGVQVFAVAYWT
                     NIWGDEYLELRDQGGGGWSTAYASTRVSDAADSYLEVFGGQYLVYAPDATEQFPSGFG
                     SDNKLFTDDDPLMPLPQGWSVVDMDQSPFAVSRDANPVMDLYEPASSALDDFSDQSYT
                     EAFDSMVDKFSHEYAWTELKGIDWTATAAEFRPAFEAAEANSSAHDYALALRDFLWSI
                     PDTHVGFDQSLLDADFITDTDGGLGFSMMETDDGTFLVDYLTPGGPADNAGIEFGAEI
                     TSIDGQDTAARVEAALPWSSPFSNPEIERLQQLRYALRFKLDKGTVEITYKNRGGAAT
                     TTSLDVVAERDSFAHSSSYFGQPDFLQPVEFRILPSGIGYIQITSFFDDQVQTVQLWE
                     EAISTLKDEGIDAVILDMRFNGGGSGWLADQMAAYFFDHRVPVGNIGRYDPTSDSFYI
                     DPTDVDEMIPPPAELQYAGQVAVLVGPACASACEFFSYNMTLEDRAEIVGQYPSEGAG
                     GSVEQFIMPEGISVQFTIGRAVDANGDVHIEGTGVAPTVRVPVTYETILEKANGGDPV
                     LSAAEELLGANP"
     gene            complement(3114..4343)
                     /gene="metK"
                     /locus_tag="VIF08_06145"
     CDS             complement(3114..4343)
                     /gene="metK"
                     /locus_tag="VIF08_06145"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY8135604.1"
                     /translation="MTSIVDDESYLFTSESVTEGHPDKMCDQISDAILDAILKDDPVA
                     RVACETATTTGTVVVLGEITTDTYVDFQAVIRDTVRNIGYTDAAYGFDYRTCGTLVSI
                     HEQSPDIKRGVDDALETRGKTDIHDQGAGDQGMMFGFACRETPELMPLPITLAHRIAR
                     RLSEVRKEGLVDYLRPDGKSQVTVEYNKGVPVAVRTVVVAAQHDDDVANDRLRDDIIE
                     MVVKQVIPEELRETMPKVHVNPTGRFVIGGPMGDAGLTGRKIIVDSYGGMARHGGGAF
                     SGKDPSKVDRSGAYAARWVAKNIVAAGLADRFELEISYAIGVAKPISISIETFGTGKI
                     DDVRILGLIDKHFDLRPGAIIESLDLRRPIYKQTAAYGHFGRPELDLPWERTDRAEAL
                     ASEAGIRMPEPVGATAS"
     gene            4404..5696
                     /locus_tag="VIF08_06150"
     CDS             4404..5696
                     /locus_tag="VIF08_06150"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8135605.1"
                     /translation="MTVQLPRKTETVIVGAGQAGLTMSWWLQQAGREHVLIERRTTLG
                     GGWQDRWDAFRLVSPNWTSSFPGYRYQGDDQHGFMPRDEIAVRVASYARHINAPVITG
                     VEVTRLAAPTGGGFDLQTTQGPVHAQNVIVATGSFHEPRLPVQAAELPRRLVQLHSSA
                     YREEGALPAGAVLVVGTGQSGVQLAEELHEAGRHVVLSVGSAGRVPRRYRGFDTLGWL
                     ARMAVHGDEVGVSLPSVETLPDPRLRFSGNPHLSGHGGGHDTNLRQFAAEGMTLIGRI
                     EGVTGERLRLAPDLAKNLAWADGFFDLNFRDLCEAFIAASNFDPPPDDRRAFAYEPPT
                     VEQLDLEKEGVNSVLWTTGFRMDFSRWIDLPLFEDNGYPRQVRGVTEVPGLYFLGLLW
                     QHNQGSATLFGVNEDAHHLARQMGLPEPSTDWKLPIPD"
     gene            complement(5728..7032)
                     /gene="ahcY"
                     /locus_tag="VIF08_06155"
     CDS             complement(5728..7032)
                     /gene="ahcY"
                     /locus_tag="VIF08_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8135606.1"
                     /translation="MTTTDLPREQTAAGNLPEHDVADLSLAPAGVTRIEWAEREMPVL
                     RLIRERFEREQPLAGMRIGACLHVTTETANLMRTLKAGGAQLALCASNLLSTQDDVAA
                     ALVAEYGISVFARRGVDRDGFYRHLNAVADTHPHLTQDDGCDLVSLLHKDRTDALAGV
                     LAGTEETTTGIIRLRAMAADGALKYPVVGVNESKTKHMFDNRFGTGQSTVDGILRATN
                     ILLAGRHVVVAGYGWVGKGIASRMRGMGAQVAIVEVDPIVAIEAFMEGYQVMAGIDAA
                     AWGEVFITATGNLHVFRREHFERMRNGAIMANSGHFDAEIDLVALDELAGGHVRQLRR
                     DVKEYDLGDRRVYVIADGRLVNLAAAEGHPAAVMDMSFANQALAAEYGAKHHKELSPA
                     VHVMPDEIDREVARLKLEALNVQIDTMTPEQERYVKSWQEGT"
     gene            complement(7032..>7889)
                     /locus_tag="VIF08_06160"
     CDS             complement(7032..>7889)
                     /locus_tag="VIF08_06160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135607.1"
                     /translation="GRARTQERAIVATPTRDKQLLRAFLERDRLRAGYAVADLEEREF
                     AKSRWGVASEGGEPIAVVLEYGGLTPQPLFVMGDPDGIVAILRDVIRPRLAYLAADES
                     HLAAVAGLYRIDAGPPMVRMAVDRRTFEPVAGQVQRLAPADIVDLNRLYGLGFAGWLP
                     ADSIANGIYYGVRIAGRLVAAAGTHVISPEGRIAAVGNVMTHIDFRGRGYAKQTTSAV
                     TQELLRTCDDVVLNVRADNPPALAAYRALGYRDYCHFEERLVHRRGSAPWDSIVAYLT
                     RALLRPRSP"
BASE COUNT         1261 a         2692 c         2619 g         1317 t
ORIGIN      
        1 caggtgcgcg gcatcaccgg ctggcacgac acgcgcgggc gcggtgtatc cacctacgag
       61 tgcgtccagc agcgccgtcc acgtgccgac atcggaccag ccgacatcca ttgcgcccat
      121 caccacctgg ccctccgcgg ctgcgggctc catgaccgcg taatcgatgg agatcggctc
      181 gatctccgcg tatgcagcct ccaggcgcga ttccgagtcg cgcgtattcg ccagcggccc
      241 aacgagacga gtgtgcttct ccagggcagc gagccacgcc ctgcggcggg ccatgaagat
      301 gccggcgttc cagcacacat ccgggatgct caacagctcg gccgccttgg cggcgtccgg
      361 cttctcgaca aagcggctga gggcgtaggc gtggagcctg ttcaactgct ggcgcgccgg
      421 atcgggcacc aggtagccgt agtgggtcga gggcccggtt ggcgtggctc ccagggtgac
      481 aatcggtgac tcgatcccga acgccccctg ggccagctcg cgttgggcat cagccagaac
      541 ggcccggaac acgtcatcgc gctggaccag gtggtccgca ggcagaacga ccatcaactc
      601 gtcggggtcg cgctcgatcg acgtcgcggc cagcgcgaca gcggcggccg tgttccgccc
      661 aacgggctcg gcgatcacgc ccacgtcggt ctggtcgctc accagagagg cgtaacgccg
      721 gtctgtgacc acgtggatgt cgtccaacgt caggttgagc tcgtcaccgg acatcaagcg
      781 cgtgacggtg cgctggagga ggctcgcgcc atccgggagc agcggcagga agggcttggg
      841 gtggcgcggg tcgctgagcg gccacaggcg cgtcccgccc ccaccggcga ggatcactgc
      901 gtacatgtgg cgcgaattct atgggccggc gacgcgctgc cgtccgtgtg ccaccatacg
      961 gcgcgtgttg agacgaattc ccccacccgt cctcgttgcg ttcatcctcg ctgccgcgtg
     1021 caccaacccc acgcccagtc cgactcccgc ccccagcggc agcgcgaacc cgactccgac
     1081 cacgagcgcc gtgccgacga ccacggcggg gcccacaacc actgccggtc caacacccgg
     1141 agcatcgggc actgggccgc aggtcgtgac gggcagcgtc agctactcca atgccttctt
     1201 cacggcgggc gtttcccagc cgctgatcat cctcgaagac cagggcggct tcgtgacccg
     1261 tgatcgcaac ttcgcgatct ccgagccgtc gcaggtcatc ggcgcgatca cgtccaactt
     1321 cttcacatcg ccgttcactt acacgctcac ccttcccgcc caacccaacg gcagcgtcca
     1381 cgacgtcgac cacgatgggc agcaggacca gggggtgcag gtcttcgccg tcgcctactg
     1441 gacaaacatc tggggcgacg agtacctgga gctgcgcgac cagggtggcg gcggctggtc
     1501 aacggcctac gcatcaacac gggtgagcga cgccgcggac tcctacctcg aggtcttcgg
     1561 cggccagtac ctggtgtacg cgcccgacgc gaccgagcag ttcccgagcg gctttggctc
     1621 cgataacaag ctcttcaccg acgacgaccc gctcatgccg ctgccgcagg gctggtcggt
     1681 cgttgacatg gaccagagcc ccttcgccgt ctcccgtgat gcgaaccctg tcatggacct
     1741 ctacgagccc gcgtcgtccg cgctggacga cttctctgac cagtcataca cggaagcctt
     1801 cgactcgatg gtcgacaagt tcagccacga gtacgcctgg accgagctca agggcatcga
     1861 ctggacggcc accgcggcgg agttccggcc cgctttcgag gcggcggagg cgaactccag
     1921 cgcgcacgac tacgcgctcg cgttgcgcga cttcctctgg tccatcccgg acacacacgt
     1981 cggtttcgac cagtcacttc tcgacgcgga cttcatcact gacacagacg gcggccttgg
     2041 cttctcgatg atggagaccg acgacggcac attcctcgtc gactacctga caccgggcgg
     2101 tccggctgac aacgccggaa tcgagtttgg cgcagagatc acgtcaatcg acggccagga
     2161 cacggccgcc agggtcgagg ccgcgctgcc ctggtcgtcg cccttcagca accctgagat
     2221 cgagcgcctc cagcagctgc gctacgcgct gcgcttcaag ctcgacaagg gcaccgtcga
     2281 gatcacctac aagaaccgcg gcggggcggc gacgacaacc agcctcgacg tggtcgccga
     2341 gcgcgattcc ttcgcccact cctcgtcgta tttcggccag cctgacttcc tgcagccggt
     2401 cgaattccga attctgccgt cgggcatcgg ctacatccag atcacgagtt tcttcgacga
     2461 ccaggtgcag accgttcagc tgtgggagga ggccatctcc acgctcaagg acgagggcat
     2521 cgacgcggtc atcctcgaca tgcgcttcaa cggcggcggt tcaggctggc tggccgatca
     2581 gatggcggcc tacttcttcg accatcgcgt ccccgttggc aacatcggcc gctatgaccc
     2641 gacgagcgac tctttctaca tcgatccgac ggacgtggac gagatgatcc cgccgccggc
     2701 ggagttgcag tacgcgggcc aggtcgcggt gctggtaggc ccagcatgcg ccagcgcgtg
     2761 cgagttcttc tcgtacaaca tgacgctcga agatcgcgcc gagatcgtgg gccagtaccc
     2821 gtccgagggc gcgggcggca gcgtcgagca gttcatcatg cccgagggca tcagcgtcca
     2881 gttcaccatc ggccgtgccg tcgacgccaa cggtgacgtg cacatcgaag gcacgggcgt
     2941 cgcgcccacg gtccgcgtgc cggtcacgta cgagacgatc ctcgaaaagg ccaacggcgg
     3001 cgacccggtt ttgagcgcgg ccgaggagct cctcggcgcg aatccctgat ctcaggtacc
     3061 ggctcgtgtg agatggacca gataggtggg acgacggcgc tgaagtcgct ccgttagctg
     3121 gccgtggctc cgaccggttc cggcatgcgg ataccggctt ctgacgccag cgcctcggcg
     3181 cggtcggttc gctcccaggg caggtccagc tcgggccgcc cgaagtggcc gtacgccgcg
     3241 gtctgcttgt agatgggccg gcgtagatcg aggctctcga tgatcgcgcc cggacgcagg
     3301 tcgaagtgct tgtcgatcag gcccaggatg cgcacgtcgt cgatcttgcc ggtaccgaac
     3361 gtctcgatcg agatggagat gggcttggcc actccgatgg cgtagctgat ctcgagctcg
     3421 aagcggtcag ccaggccggc ggccacgatg ttcttggcca cccagcgggc ggcataggcg
     3481 ccgctgcggt cgaccttcga cggatccttg ccggagaacg cgccaccacc gtggcgggcc
     3541 atgccgccat atgagtcgac gatgatcttg cgcccggtca ggccggcgtc gcccatgggg
     3601 ccgccgatga cgaaccgccc ggttgggttg acgtgcacct tgggcatcgt ctcgcgcagc
     3661 tcttcgggga tgacttgctt cacgaccatc tcgatgatgt cgtcgcgcag ccggtcgttg
     3721 gcgacgtcgt catcatgctg cgccgcgaca accacggtac gcacggcaac gggcacgccc
     3781 ttgttgtact cgacggtcac ctgcgacttg ccgtcaggcc gtagatagtc gacgaggcct
     3841 tccttccgca cctccgacag gcgacgggcg attcggtggg cgagcgtgat gggcagcggc
     3901 atcagctccg gtgtctcgcg gcaggcgaag ccgaacatca ttccctggtc gcccgcaccc
     3961 tggtcgtgga tgtcggtctt gccgcgcgtc tcgagcgcgt cgtcgacgcc gcgcttgatg
     4021 tccggcgact gctcgtggat cgagaccagc gtgccacagg tgcggtagtc gaagccgtac
     4081 gcggcgtcgg tgtagccgat gttgcgcacg gtgtcgcgga tgaccgcctg gaagtcgacg
     4141 taggtgtccg tcgtgatctc gccgagcacg acgacggtgc cggtggtcgt cgcggtctcg
     4201 cacgcgacgc gcgcaacggg gtcgtccttc aggattgcgt ccaggatcgc gtcactgatc
     4261 tggtcgcaca tcttgtccgg gtgaccctcg gtcactgatt cggacgtgaa caggtagctc
     4321 tcgtcatcca cgattgaggt catgaaagtc agggtaaccg atcagccgac atggcctcca
     4381 gcccagaggt accctgcccc gctatgaccg tgcagctgcc gcgcaagacc gaaaccgtga
     4441 tcgtgggtgc gggccaggct ggcctgacga tgagctggtg gctccagcag gcgggccgcg
     4501 aacacgtcct gatcgagcgc cgcacgacgc tcggtggcgg ctggcaggac cgctgggacg
     4561 cgtttcggct ggtgtcgccc aactggacat cctcgttccc gggctatcgc tatcaaggtg
     4621 atgaccagca cggcttcatg ccgcgcgacg agatcgcggt gcgcgtcgcc agctacgcgc
     4681 ggcacatcaa cgccccggtc atcacgggcg tcgaggtcac tcgcctggcg gcaccgacag
     4741 gcggagggtt cgacctccag acgacccagg ggccggtcca cgcccagaac gtaatcgtcg
     4801 ccacaggcag cttccacgag ccgcgcctcc ccgtgcaggc cgcggagctg ccgcgccgcc
     4861 tggtccaact gcattcctcg gcatatcgcg aggaaggtgc gcttccggcg ggtgctgtgc
     4921 tggtggtcgg gaccgggcag tcgggcgtgc agctcgccga agagctgcac gaggccggcc
     4981 gccacgtggt tctgtcagtc ggcagcgccg gcagagttcc gcggcgttac cgcggcttcg
     5041 acacactggg ctggctggcg cgcatggccg tgcacggcga cgaggtgggt gtgtcgctgc
     5101 cgtcagtcga aacgttgccg gacccgcgcc tgcgcttttc gggtaacccc catctttccg
     5161 gtcatggcgg cggccacgac accaacctgc gccagttcgc ggccgaagga atgaccctca
     5221 tcggccgcat cgagggcgtg accggtgaac ggctgcgtct cgcgcccgat ctggccaaga
     5281 acctagcctg ggccgacggc ttcttcgacc tgaacttccg cgatctgtgt gaggcgttca
     5341 tcgccgcatc caacttcgat ccgccgccag acgatcgtcg agccttcgcc tacgagccgc
     5401 caaccgtcga acagctcgac ctggagaagg agggcgtcaa cagcgtcctc tggacgaccg
     5461 gcttccggat ggacttcagc cgctggatcg atctgccgct cttcgaggac aacggctatc
     5521 cacgccaagt gcgcggcgtt accgaggtgc cgggactgta cttcctgggt ctgctgtggc
     5581 agcacaacca gggctcggcg acgctgttcg gcgttaacga ggacgcgcac cacctcgcgc
     5641 gccagatggg tctacccgaa ccgtctaccg actggaaact gccgatcccc gactagcgac
     5701 ttttcgttcg ctggccagcg aaggtaatta agtcccttcc tgccaggact tcacgtagcg
     5761 ctcctgctcg ggcgtcatgg tgtcgatctg gacgttgagc gcctcgagct tgaggcgcgc
     5821 gacctcgcga tcgatctcgt caggcatgac gtgcaccgcg ggcgagagct ccttgtggtg
     5881 ctttgcgccg tactcggcgg cgagcgcctg gttggcgaag ctcatgtcca tgaccgccgc
     5941 cggatggccc tcggcggccg cgaggttgac cagccggccg tcggcgatga cgtacacgcg
     6001 ccggtcgccc aggtcgtact ccttgacgtc gcgccgcagc tggcggacat gcccgccagc
     6061 cagctcgtcg agcgcaacga ggtcgatctc cgcgtcgaag tggccgctgt tggccatgat
     6121 cgcgccgttg cgcatgcgct cgaagtgctc gcgccggaaa acgtggaggt tgccggtcgc
     6181 ggtgatgaag acctcacccc aggccgcggc gtcgataccg gccatgacct ggtagccctc
     6241 catgaacgcc tcgatcgcga cgatcggatc gacttccacg atggccacct gggcacccat
     6301 gccgcgcatc cgcgacgcga tgcccttgcc cacccagccg tagccggcga ccaccacatg
     6361 gcggccggcc agcaggatgt tggtggcgcg caggatgccg tcgaccgtcg actggccggt
     6421 gccgaagcgg ttgtcgaaca tgtgcttcgt cttggactcg ttcacgccta cgaccgggta
     6481 tttgagcgcc ccgtcggcgg ccatcgcgcg caggcggatg atgcccgtcg tcgtctcctc
     6541 ggttcccgcc aacacgccgg cgagcgcgtc ggtgcgatcc ttgtgcaaca gggacaccag
     6601 gtcgcagccg tcgtcttgcg tcaggtgcgg gtgcgtgtcg gcgactgcgt tcagatggcg
     6661 gtagaagccg tcgcggtcga cgccgcggcg ggcaaagacg ctgatcccgt actcggccac
     6721 gagcgccgcg gcgacatcgt cctgcgtgga cagcagattt gaggcacaga gcgcgagctg
     6781 cgcgccgccg gccttcaggg tgcgcatcag gttggcggtc tctgtggtga cgtgcaggca
     6841 cgcgccgatg cgcatgccgg cgagcggctg ctcgcgctcg aagcgctcgc ggatgaggcg
     6901 tagcacgggc atctcgcgct cggcccattc aatgcgtgtc acaccggccg gggcgaggct
     6961 caggtccgcg acgtcatgct cgggcaggtt gccggcggcg gtctgctcgc gcggaaggtc
     7021 ggttgtggtc actagggact ccttgggcgc agcagcgcac gcgttaggta ggccacgatg
     7081 ctatcccaag gggcgcttcc gcgacgatgg acgagccgct cctcgaagtg gcagtagtcg
     7141 cgataaccca gcgcgcggta ggcggccagc gccggtgggt tgtccgcgcg cacgttgagc
     7201 acgacgtcgt cacaggtgcg cagcagctcc tgggtgaccg cgctggtcgt ctgcttggcg
     7261 tagccgcgac cgcggaagtc gatgtgcgtc atcacgttgc cgacggcggc gatccggcct
     7321 tcgggactga tgacgtgcgt gcccgccgcg gccacgagcc gaccggcgat gcgcactccg
     7381 taatagatgc cgttggcgat ggaatcggcg ggcagccagc cggcgaaacc aaggccgtac
     7441 agccgattga gatcgacgat gtcggccggt gccaggcgct ggacctgacc ggccacgggc
     7501 tcgaacgttc gccgatcgac cgccatgcgc accatcggcg gcccggcgtc gatgcggtac
     7561 aggccggcca ccgccgccag gtgactctcg tccgcggcga ggtacgcgag gcgcggccgg
     7621 atcacgtcgc gcaggatcgc gacgatgcca tctgggtcgc ccatgacgaa cagcggctgc
     7681 ggcgtcaggc cgccgtactc gaggacgacc gcgatcggct cgcctccctc gctcgccact
     7741 ccccagcgcg acttcgcgaa ctcgcgctcc tcgagatctg ccaccgcgta gccggcgcgc
     7801 agccggtcgc gctccaggaa ggcgcgcagc agctgtttgt cgcgcgtcgg tgtcgccacg
     7861 atcgcgcgct cctgcgtccg ggcacggcc
//
LOCUS       DATBRX010000163        25879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1513295, whole genome shotgun sequence.
ACCESSION   DATBRX010000163 DATBRX010000000
VERSION     DATBRX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 25879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25879
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513295"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VIF08_06165"
     CDS             complement(<1..1035)
                     /locus_tag="VIF08_06165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8135608.1"
                     /translation="MTDTLPRPYTEAADDAVERLLETQSPQRRLSSPTFTVEVDGKQF
                     TGRAGQTILEICRDNGIDVPTLCYEPKLPGFGACRMCVVDVAGEENPPISCSRAAEPG
                     MVIQTQTPRLREIRKTNLELIFSDHNAYCLPPCQNKCPSHIDIPGFLKANTEGNFAES
                     ARIFKRTIPFPSVLGRVCPAPCEEHCRRDEVEEAIAIRDSHRYAGDQVLAAQAKGIPA
                     PVPFEIQKKTGKRVAVIGSGPAGMSAAYYLLIAGHDVTIYERDPAPGGMLRYGIPQYR
                     LPKVEILEAEYQSVTDLGGQMVCNAELGKDFTIDDLQNQGFDAVCVAIGCYETNKLGV
                     PGEEADGVLDG"
     gene            complement(1032..2351)
                     /locus_tag="VIF08_06170"
     CDS             complement(1032..2351)
                     /locus_tag="VIF08_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY8135609.1"
                     /translation="MKMLASPKGVPQILTGRFGKYDADGPLSGAEAAGAWSAWKKAAT
                     SLSPGAVIRTVSEAGLRGRGGAGYPAADKWRAAAAVDADRRYVVANGFEADPGAMVDR
                     TLMENDPHAIVEGVAFAAFATGASHAYIAVRANLSTALKRLTAAVRAAEEAGYIGSDA
                     LGAGIDIHVEVVAIQGGMVVGEETTLLHAIENKRAQPDQRPPYPSGRGLWDRPTVVNN
                     VATLASVPWIFANGAQAYAAVGDPDYPGTTLVQFAGALKTPGIVEVPLGMSVRKLLDI
                     AAPQATLKAVLVGGPAGGFLPPAEMDTLYTPQALVAKGAMCGSGTVLAIGPETCLVEM
                     ATTLERFLSDEACGKTIPCRIGLRRMYEMGARATTGLAAPDDAKNLVDLGNDIRAAAL
                     CGLEYCAPNPFLTGMRFFADEFEAHFSRGTCPAGVCESVRVAQTVMA"
     gene            complement(2371..3204)
                     /locus_tag="VIF08_06175"
     CDS             complement(2371..3204)
                     /locus_tag="VIF08_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY8135610.1"
                     /translation="MSKVPGWGMAKGMVYTFKRFVQPKITIQYPEQRQDISPHHRGRL
                     VLLYDEAGNLKCETCFQCAMACPIEIIDMGGVDTKNRMHVHWGPPEQYAERREESALR
                     RSGRPVADRAFAAWLPIDLGPLERILAANDYDPHRLLAMLDQTQSTYGYLPVAALQHI
                     SHSTGAWYSEIYGIATSYPHLVFEPGRVAEIGLCRCAACSLAGGGRVRDALVDALGVE
                     VGGVTADGSVRFSAADCGGDNGGQPLVTLDGQRQPGMTADKARDLAQKLRAKSRAAAT
                     A"
     gene            complement(3233..4624)
                     /locus_tag="VIF08_06180"
     CDS             complement(3233..4624)
                     /locus_tag="VIF08_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEY8135611.1"
                     /translation="MSVISRPWNRLSLAGKIILPLFLLLVVLTVLLLAGWQLGLIPAV
                     IELLAELVRSNIDIFRFIVTATATLLITIPVAFVIIYMEMKVIAFLAGRIGPNRVGPW
                     GTLSSVFHGFKVLAKEDFTPTGADAAVFTVAPIITYLSSVLTLLVIPFAPGLVGLDMN
                     IGLLYFFAVSGLTVVGLLTAGWSSFNKYSLLGGLRAAAQIVSYELPLLLAPVGIIMLA
                     GTLSLNQIVIQQSGWFWDWYVFQQPIAFIVFFIAAAAEGNRTPFDLTEADSEIVAGFA
                     TEYSGMRFGFFFFAEYVNVFILSALTVVLFFGGWNAPIDPRPILDFFHIGSLTPAIDV
                     SSMGIWLLWVLGIGVPLLVLLVALPLWMLKSGWSFLKSLVVAFVLFNVFAIAAVLVWA
                     FLSFEVVVGLFWFIVKTFTFVFIFVWMRGTLPRVRIDQLMGFAWKWLVEIALLNIFVT
                     AGAILLVQELARQ"
     gene            complement(4621..5844)
                     /locus_tag="VIF08_06185"
     CDS             complement(4621..5844)
                     /locus_tag="VIF08_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135612.1"
                     /translation="MTTDRGSVRSLDDPNIWVETPATIFDAVPPYPPRTEREAEFYNL
                     RDGEMLLNLGPQHPSTHGVLRIVLKVDGERVTDLDPVLGYLHRGVEKICENGDWHHAI
                     SNCDPLEYVASMFSEAMPVLSAEKLLDLQVPRRAEYIRVLCWELNRIASHGLFIGWLA
                     LDLGGLTPLLYGFIERDRIVEMLASLTGQRMLFNYLRIGGVNRDLNHDFLSRLGDWMS
                     KAAVELENETRLLNENEIFVRRTTGMGTLDRDTALRLCVTGPNLRAAGVPYDVRRAHP
                     YSVYPELDFDIPTRDAGDSYARYLLRVDEIRESIRIIDQVLHEMPDGPIMAKIPRMIR
                     IPPGRAFISVESPRGQYSCYAMSDGSDQPFRLRIHDPSYFTLQAVGHLMPGNLIADSM
                     AIMASLDPVMGGVDK"
     gene            complement(5841..6386)
                     /locus_tag="VIF08_06190"
     CDS             complement(5841..6386)
                     /locus_tag="VIF08_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY8135613.1"
                     /translation="MIQKGDTTEIWIGAADVTDVMKTLHDDEQFEFELLADLAGVDTG
                     TEMWVVYHLWSPRSPDWLRVIAAGLSREDPRVPSVTFIWKGAEWAEREAYDMFGIVFE
                     GNRDLRRIYMPPDYTSFPLRKDFLLPDDAARSPGEGVRHVEQAFPPAEQLRQPVLRRA
                     ELGSTTPGSAAELAAGTEQES"
     gene            complement(6461..7177)
                     /locus_tag="VIF08_06195"
     CDS             complement(6461..7177)
                     /locus_tag="VIF08_06195"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEY8135614.1"
                     /translation="MEVNKPIEMGSDQPGNTPGALAVADKSEVVEPIIVENRLWTPVN
                     PNAYDGGRPAQITHLRAEEHADKGDTGRWRALDIIPTKADYILDLIRLNSLWPLTSGL
                     ACCAIEMMSAATPWNDMDRFNMFPFRASPRQADVLIVAGTLTTKMAGPLVRLWEQMPE
                     PKWCVAMGDCTCSGGRYKRSYATVEGIDRVMPVDVYVPGCPPRPEGLIYGMMKLQQLI
                     RERRGHWAERAVGPTVPDGV"
     gene            complement(7168..7527)
                     /locus_tag="VIF08_06200"
     CDS             complement(7168..7527)
                     /locus_tag="VIF08_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY8135615.1"
                     /translation="MTTGLWYVIGFAFAGTTFVLITIIASWLLSHRTKGDRHKGMPYE
                     SGIDTYGYTWGRTGLSFYIYALLFVAFDIEVVFIYLWAIVFRDVLLGGFLAMLLFVFI
                     LLIGLGYAWRKGVLTWK"
     gene            7713..7859
                     /locus_tag="VIF08_06205"
     CDS             7713..7859
                     /locus_tag="VIF08_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135616.1"
                     /translation="MPAKSEKQRKFMGAELARKRAGKKTQTGMSEKKLEEFASKPGGG
                     KKKS"
     gene            complement(7856..8938)
                     /locus_tag="VIF08_06210"
     CDS             complement(7856..8938)
                     /locus_tag="VIF08_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135617.1"
                     /translation="MTRPAVLIFHRPAAVGDPPLVRFLADVRAELVRRQAALLERAGA
                     APPTFVDEWYEDLAFGDLIRRLAPTHTGVIVLGSGAVARLNLADARRLVAVAASGRAE
                     ALTNNRYSSDIVALGRASMLKKAPALPSDNALPRWLEELAGFKVDELTGRDRLALDLD
                     TPLDVCLAAFAPGAPAWLRRAVDDAGLAIPRLDDLRALVRDPLRELLVFGRSGSRTLA
                     WLERNVRCRVRFLAEERGLRASSQLAIAGKAAGTAKTTSRKPSSTLSLLLEQRGPEAL
                     SEIIAQLADAAIIDTRVLLAARLGSDESGWPTPPDRFASDLHDHAAVSDAWLRELTRS
                     AADSPRPILLGSHTLVGPGVRLLVGP"
     gene            8971..9540
                     /gene="folK"
                     /locus_tag="VIF08_06215"
     CDS             8971..9540
                     /gene="folK"
                     /locus_tag="VIF08_06215"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEY8135618.1"
                     /translation="MTKRVRAKRVRAYVGLGANVGDTRANLRHAVRAMTALPGVKVRG
                     VSRLYRTKPVGVTDQPDFLNAVAALEVPAGPDPATGALALLSVLKVLEAAIGRKKRQR
                     WGPREIDLDLLVFGRHRIHVERPHNQAEKRWLEVPHPEMRRRLFVLAPLADLAPWLVP
                     PGWGESVETARRRIAANEPENAVQVVGKL"
     gene            complement(9519..11000)
                     /locus_tag="VIF08_06220"
     CDS             complement(9519..11000)
                     /locus_tag="VIF08_06220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY8135619.1"
                     /translation="MSQFRYGVASGDPAPDRVVLWTRLERGGSLRWEVAEGSDFQSVV
                     NSASVESTPDSDFCVSVDVGGLKPCTTYWYRFRAPDGEWSPVGRTKTLPVGDARRVRF
                     AVVSCAKFNAGFFNGYARIAERDDLDFVLHLGDYIYEASETPPANQTPGADIGRPFEP
                     RNECVTLADYRTRYAQYRADPDTQAMHAAHPLIATIDDHELADGGWRDGADNHLAERD
                     GPWPVRRAAAFKARREWLPQRLPDPADPLRVYRSVSLGDVADMFVIDTRSLRDQPVHG
                     PGAVAPARSQLGHAQRDWLFAGLQQSRAQWRLLANSSILGRTWAPNLPPELHEGLTYL
                     KLIHKSGTGPDPDQWEGYPYERDLLIGHLARANTVVLSGDIHVGMALELERDEEPGKW
                     VAPEFVTTSLTSQNLDDKTGWGYRTRSTEVERLFVSARPNVAWCDMDSHGYMVADLTP
                     DRLRIEWWFVDTVLARSPNERLAAYAEVLPGSPEIVNYSFPTT"
     gene            complement(11010..11987)
                     /gene="glpX"
                     /locus_tag="VIF08_06225"
     CDS             complement(11010..11987)
                     /gene="glpX"
                     /locus_tag="VIF08_06225"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY8135620.1"
                     /translation="MEGLLALEMVRVTEAAAIASARHMGRGDNQAADHAAVEAMRKTF
                     DDVDLAGTIVIGEGERDKAPMLYIGESVGRSMPGQPEIDIAVDPLEGTNLVAHGNSNA
                     ITVIAASEKGGLLNAPDTYLEKLCVGPDVAGKVDIRLTPTENLDRIANILGRKVADLT
                     VVILDRPRHAQLIDEVRAAGARIKLISDGDLSAAISCAVRGTGVHAVMGTGGAPEGVL
                     SAVALRCLGGEIQARLRWRSDEERDRAVAMGVDPSMERVYTTSDLAPGTNLVFAATGV
                     TDGDLLGGVRFFGGGARTHSLVMGYQSKEVRFIDTVHMFDRDKPPAVRL"
     gene            complement(11996..13435)
                     /gene="purH"
                     /locus_tag="VIF08_06230"
     CDS             complement(11996..13435)
                     /gene="purH"
                     /locus_tag="VIF08_06230"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY8135621.1"
                     /translation="MRALLSVANSDGVADLARDLAALGVELHADADALAVLAQAAVSA
                     QPVEKAGASAEGFDIVVANIKPFAPQVGERVVPLDEAIAMIDVTRTSLMAAAARNLAS
                     VAIVSGARHYPQLLQELRTRNIVSAEFRQHLAADALADVSAYFAEVAGYLNHLGGNRF
                     PNRLAVVVEKVRDLGYGENPQQHAAFYRESMHRSGALADAHQLQGDQPTFNDLIDLDA
                     AYRIVSDFTAPTCCIVKQNNPTGLASDELLVHAYQRALEGDPVSAYGAVVALNREVDG
                     NTAEALAGNAYEAIVAPGFTAPAREILAEKQRLALLAVPHRFTEGLSDYGIARLDFAR
                     IDGGLLVETRDSSEVDRSQLKVVTQRRPTLEELTDLLFAWRAVRHVTSNAVVLAHHTA
                     LVGVGAGQASRLTAVEIALHKAAERSKLAVLASDAYFPFADAIQLAAERGVTAIIQPG
                     GSVRDEMAIEVANRHHLAMVFTGKRHYRH"
     gene            13573..14034
                     /locus_tag="VIF08_06235"
     CDS             13573..14034
                     /locus_tag="VIF08_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135622.1"
                     /translation="MKVSLLEDLFWYLNKPFVGGQPESGGFPDIFHPIWIAAAVMLVL
                     TVVLYNVRVRQLHRHEPLRSMQEWLLWTGLVTFGLVIVGTVFRFYFLFVLLFIVIGLA
                     AFVWIRFVHFPPYIEAYNEQLRRARFFSQSKYKAAESTVRVKRNRGGRRRR"
     gene            14135..14587
                     /locus_tag="VIF08_06240"
     CDS             14135..14587
                     /locus_tag="VIF08_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8135623.1"
                     /translation="MEIRRFGPGHRRAEGPPGTKGVAGALLLSDDRAVITEVAFGPYA
                     MIQPHANQSNTALFIVISGGGFVQVGEERARVSHGEAVIWPPGILHGAYTDGTEMRAI
                     IVELVGDGTAVVEGRAHAAESAATKAKGALAERQRTRDEYDSSEGEPW"
     gene            14773..15405
                     /locus_tag="VIF08_06245"
     CDS             14773..15405
                     /locus_tag="VIF08_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135624.1"
                     /translation="MTHLDDQDGRVAAIDVQPLTTERFADLAELFNEGGDPKWCWCQY
                     FRKAGLDWTNSTTAGNRAALESLAARPDLAPGLVGYRDGRVVGWVSLAPREDYGRLEN
                     AKVLARVDDTPVWSIVCFVVSRKARGQGVATALLDGAIAYAREHRATMLEAYPVAATR
                     GRISPASAFHGVQSMYEKAGFAVVATRQWNETSPIRPIMRLALGAQGRKR"
     gene            15402..16004
                     /locus_tag="VIF08_06250"
     CDS             15402..16004
                     /locus_tag="VIF08_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8135625.1"
                     /translation="MKLFATMMLSVDGVYQGPGGPDEDRRGGFERGGWTAASASEEMG
                     PYLTEMFERADALLLGRQTWEIWEPYWPKHDDNPFGHMINVLPKFVTSTTLKDPTWQN
                     THVLSGDVEAQIRELKGRPGREIQVHGSGVLLRWLLERDLVDELNLRISPVIVGDGLR
                     LFPERGPTHNLELVDSWTTKTGVLVQTYRPAGRATFGPIG"
     gene            complement(16016..17176)
                     /locus_tag="VIF08_06255"
     CDS             complement(16016..17176)
                     /locus_tag="VIF08_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEY8135626.1"
                     /translation="MAEFDMSLVQRGARLRHSPYYEATQAYSPKGFTVYNHMLFPIRF
                     ESFEEEFETLLNDVTVWDVAVERCLEISGPDGFRFAQLLTPRDLSKCAVGQAKYVLIC
                     DGDGGIINDPVLTRMDENTFWFALASSDALLFARGLRHAYPDLDVTIREVDVAPMQIQ
                     GPKSKNLMHDLVGDVVDDMKYYFWTRAEVNGIPVVITRTGWTSEVGYEIYLLDRSKGT
                     QLWQAVMEAGKPYGVKPTGPSDIRRIEGAIFNWGADMNYDNNPLEMGLERLIQWELPD
                     SASISMAALRRIKAAGVTRKIVGVEMDGDPFPALNFNKWDAFRDGGSDAVGQVSSAIY
                     SPRLKRNIGFCWVPVDLSAPGSTLRVASEWGDRHATVVPMPFVDPEKKIPIS"
     gene            complement(17180..17836)
                     /locus_tag="VIF08_06260"
     CDS             complement(17180..17836)
                     /locus_tag="VIF08_06260"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8135627.1"
                     /translation="MKFEEAQARLAQVPDRLPPPPPELMPVVVADPEGVPGGAPRFPD
                     GPRREAAVLILIYPDANGDAYLILTERSPGDHRHAGQISLPGGAIDAADPTPAAAALR
                     EAAEEVGLDPVEAGVTIAGVLPLADVRVSGFMVAPVVAFAQRPPTLTPDGYEVASVFG
                     APLAAFVKGAPIEIITDERDGYRLRYGAYRVGEHLVWGATAMMLGRLGAFLGQAAERR
                     "
     gene            complement(17836..18894)
                     /locus_tag="VIF08_06265"
     CDS             complement(17836..18894)
                     /locus_tag="VIF08_06265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8135628.1"
                     /translation="MRAEPVRSSLAAPPADDPGEPRLLEAILAEIDERGPITFARFME
                     RALYEPGLGYYARSRERTTRSGDFLTAPELHPIFGQTVVRQIERMRQQLGRPIDFTVR
                     EYGAGRGTLGQALPPGVSYEPIEFDQPRPASRLTGVMLANEFLDALPVHRVVAREDGL
                     RELRVGRSEGRLVEVETELSDPRLAGWFDGRQIRLADGQRAEVNLAMLDWLAEVSRDL
                     ERGYVLIFDYGLAAADLYGPDRATGTMRAFRGQHVSSDVLGSVGHQDITAHVDLDALE
                     DGARAVGLEVIARTRQAQFLLGNGLDEVYRALRDAAEADWDSALTLRAAVARLLDPQG
                     MGGFAVEILGRDAPALAG"
     gene            18931..19335
                     /locus_tag="VIF08_06270"
     CDS             18931..19335
                     /locus_tag="VIF08_06270"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8135629.1"
                     /translation="MTHIEHHSLPAGLAPATGYSHAVSGTGRLVAIAGQVAMDENGEL
                     VGPGDPQAQAERVFANLALALEAAGATFSDVIKFGVFVTDIAILPVVREVRNRYLDTA
                     GPPASTAVQVSALFRPGYLLEVEALAVVPVDK"
     gene            19332..19697
                     /locus_tag="VIF08_06275"
     CDS             19332..19697
                     /locus_tag="VIF08_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY8135630.1"
                     /translation="MTLDRKLADRALCYLETIGRVTGRPHVIEMWFAPDPARDRLYMM
                     AGGRDEADWVRNVRHDGRVRIRLGKTWLSGRAAVIEGTADEPMVRRLLAAKYQGWTDG
                     RPLSHWAQTALPVAMELDG"
     gene            complement(19694..22399)
                     /gene="clpB"
                     /locus_tag="VIF08_06280"
     CDS             complement(19694..22399)
                     /gene="clpB"
                     /locus_tag="VIF08_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY8135631.1"
                     /translation="MKLDRYTEKAQQAIVAAQRLATDADSPVLDAEHILAALLDDAEG
                     IAAATLRALGADPAQVGVEIAAALSHRAKVAGGSMSLDPRAQELIRRAEEEAQRLHDE
                     YVSTEHLLLAAAQSGGDAQRILEAAGAGHEQILGALQKVRGGQRVTSANPEGTYQALE
                     KYGRDLTRDAREGKLDPVVGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEGLA
                     QRIVRGDVPEGLKDKRVVALDLGALIAGAKYRGEFEERLKAVLKEIKDSEGQVVLFID
                     ELHTVVGAGAAEGAMDASNLLKPMLARGELHTIGATTLDEYRKHIEKDAALERRFQPV
                     YVDQPSVEESISILRGLRERYENHHGVRITDSALVAAAVLSNRYISERFLPDKAIDLV
                     DEAASRLRMEMDSMPVELDELERRRIQLEIEREALRKERDDASKARLKALEKELADIG
                     ERAGALKSQWEQEKAKIADLRATKKQLEDLQLEIDKAERAADYGKAAELKYGKLPEAQ
                     KRLADEEAALAQSGAQRLLKEEVDADDIARIVSAWTGVPVTRLLEGEQAKLIRMEDEL
                     HKRVVGQDEAVAAVSDAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALANFLFD
                     DEAALTRIDMSEYMEKFSVSRLIGAPPGYVGYDEGGQLTEAVRRRPYQVVLLDEIEKA
                     HQDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNVGSQFITGFAGRRDEESYEQM
                     KTQVTDTLRQVFRPEFLNRIDEIIVFHALTEDDLAHIVELLLADLTRRLTEHDLTLEV
                     TPAARQLIAAEGYDPVYGARPLKRSIQRLVENPLARALLEGKFKPGTTIKVDADAGSG
                     TLLFTSGGETVVANATDERRDVRRAAQREPEPEAAPAGGATERLN"
     gene            complement(22396..23406)
                     /locus_tag="VIF08_06285"
     CDS             complement(22396..23406)
                     /locus_tag="VIF08_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8135632.1"
                     /translation="MEYRDYYATLGVPRTASQADIKKAFRKLARQHHPDANKGKAAAE
                     SRFKEINEANEVLSDPEKRKLYDQLGSNWGAYQQAGGSGAGGGNPFAGFGGFGGGAPG
                     GVRFEYRGNAEDLAGFSDFFRTFFGGGGFEAESGGGGRTRTKARTRGGQSIEDLLAGM
                     NLGGNGTGRAAVPARLEAQAEVTLEEVASGTKRLLDVDGKRIEVTIPQGVANGQRIRF
                     SGVAAGADAYVKVSVAPHPVFTRDGANLTREVPITLREALLGGETAIKTLTGKVMLRI
                     PPETQTGRTFRLAGQGLPHFRKEGRGDLFVKVRVVLPSGLSEEAKRAAERFLDLAEGH
                     KK"
     gene            complement(23470..>25879)
                     /gene="lon"
                     /locus_tag="VIF08_06290"
     CDS             complement(23470..>25879)
                     /gene="lon"
                     /locus_tag="VIF08_06290"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY8135633.1"
                     /translation="VIFPEMIVPLQVGRDKSVQALNKATADSTPIALVTQRQADREDI
                     NDPSELYEVGTLAKIAQVVQLQDGTVRAIVQGQQRIKLLGFVNADPYILARVEMAEEQ
                     PASGLEVQALVRTVQGQIEQYVQSGAPVPPEAAVAARNITEPGLLADMIAYSPDMSTE
                     QRQELLETFDVFERLKLVSNFLGRQIEILELKGKIQSEVKSEMDKTQREYILREQLKA
                     IQRELGEDDPQQAEVAELREKVEQAGMPEEIKARAIKEVDRMSRIPSASPEVGVIRTY
                     VDWLISLPWNVTTDDRLDLKEAAKILDEDHFGLEKIKERILEYLAVRSLADKIRSPIL
                     AFVGPPGVGKTSLGKSVARAMGRKFVRMSLGGIHDEAEIRGHRRTYIGALPGRIIQNV
                     KTAGTNNPVFMLDEVDKIGMDFRGDPSSALLEVLDPEQNNTFQDNYLEVPFDLSKVLF
                     IATGNLLDPIPPALRDRMEIIQLPGYTQQEKIEIGKRFLIPKQLENHGLTPKNVEFTD
                     EAMVELVQAFTKEAGVRNLEREIANIMRKLARQVVEGRKRKVTVDVKKLNELLGPPRY
                     EYGELEKEDQTGAATGLVVTEVGGDVLSIEVTTMDGKEDFILTGQLGEVMRESARAGL
                     SWIRAHASQLGIAREVFEKKTLHIHVPAGGIPKDGPSAGITMALAMVSALTGIPVRKD
                     VAMTGEITLRGRVLPIGGLKSKILAAHLAGAREVILPLKNQKDLLDVPDEIRKQMKLV
                     MVESMDQVLEHALRRAPKALEAKPAEVVEQGEPVDEESPAAPDKMRRGFPGQDQPPAI
                     ASTH"
BASE COUNT         4048 a         8770 c         8807 g         4254 t
ORIGIN      
        1 gccgtcgagc acgccgtcgg cctcctcgcc cggcacgccc agcttgttgg tctcgtagca
       61 gccgatggcg acgcacaccg cgtcgaagcc ctggttctgc agatcgtcga tggtgaagtc
      121 cttgcccagc tcggcgttgc ataccatctg cccgccgaga tcggtgaccg actggtactc
      181 ggcctcgagg atctcgacct tgggcagccg gtactgcggg atgccgtagc gcagcatgcc
      241 gcccggtgcc gggtcccgtt cgtagatggt cacgtcgtgg ccggcgatca gcaggtagta
      301 ggcggcggac atgccggccg ggccggagcc gatgaccgcg acgcgcttgc cggtcttctt
      361 ctgaatctcg aacggcacgg gcgccggaat gcccttggcc tgcgccgcga gaacctggtc
      421 gccggcgtaa cggtggctgt cgcgaatcgc gatcgcttcc tcgacctcat cgcgccggca
      481 gtgctcctcg cacggcgccg ggcagacgcg cccgagcacg gaggggaagg ggatggtgcg
      541 cttgaagatg cgcgccgact cggcgaagtt gccctcagtg ttggccttga gaaagcctgg
      601 gatgtcgatg tgactggggc acttgttctg gcacggcggc aggcagtagg cgttgtggtc
      661 ggagaagatc agctcgaggt tggtcttgcg gatctcgcgc aggcgcggcg tctgggtctg
      721 gatcaccatc cccggctcgg cggcacgcga gcacgagatg ggcgggttct cctcgcccgc
      781 gacgtcgacc acgcacatgc ggcacgcgcc aaagccgggc agcttgggct cgtagcacag
      841 cgtgggcacg tcgatgccgt tgtcgcggca gatctccagg atcgtctggc ccgcgcggcc
      901 ggtgaactgc ttgccgtcga cttcgaccgt gaaggtcggc gaagacaggc ggcgctgcgg
      961 cgactgcgtc tccagcagcc gctcaaccgc gtcgtcggcg gcttccgtgt acggccgggg
     1021 cagcgtgtcg gtcatgccat caccgtctgg gccacgcgca cagactcgca cacaccggca
     1081 ggacaggtgc cgcggctgaa atgcgcctcg aactcatcgg cgaagaagcg catcccggtc
     1141 aggaacgggt tcggggcgca gtactccagc ccgcacagcg cggcggcgcg aatgtcattg
     1201 cccagatcca cgaggttctt ggcgtcatcc ggcgcggcca ggccggtcgt cgcgcgggcg
     1261 cccatctcgt acatgcggcg caggccaatg cggcacggga tcgtcttgcc gcacgcctcg
     1321 tcggacagga agcgctcaag ggtcgtcgcc atctcgacca ggcacgtttc gggcccgatt
     1381 gccagcacgg tgcccgagcc gcacatggcg cccttggcga ccaatgcctg cggcgtgtac
     1441 agcgtgtcca tctcggccgg cggcaggaaa ccgcccgccg ggccgccgac cagcacggcc
     1501 ttaagcgtcg cctggggagc ggcgatgtcg agcagcttgc gcacgctcat gcctagcggc
     1561 acctcgacga tgcccggcgt cttgagcgcg cccgcgaact ggacgagcgt cgtgcccggg
     1621 taatcgggat cgcccacggc ggcgtacgcc tgcgcgccgt tggcgaagat ccacggcacg
     1681 gacgcgagcg tggccacgtt gttgacgacc gtcggtcgat cccacaggcc gcggcccgac
     1741 gggtagggcg gacgctggtc aggctgggcg cgcttgtttt cgatcgcgtg cagcagagtc
     1801 gtctcctcgc ccacgaccat gccgccctgg atggccacga cctcgacatg gatgtcgatg
     1861 ccggcgccga gcgcgtcgct gccgatgtag cccgcctctt ccgctgcccg cacggcggca
     1921 gtcaggcgct tgagcgcggt ggacagattg gcgcggaccg cgatgtaggc gtggctcgcg
     1981 ccggtggcga atgcggcgaa cgcgacacct tccacgatcg cgtgcgggtc gttctccatg
     2041 agtgtgcgat cgaccattgc gcccgggtcg gcctcgaagc cattggccac gacataacgt
     2101 cgatctgcgt ccacggcggc ggcggcgcgc cacttgtcgg cagccgggta gcccgcgccg
     2161 ccgcggccgc gcaggcccgc ctcggacacc gtccggatga ccgcaccggg gctcagcgac
     2221 gtcgccgcct tcttccaggc tgaccacgcc ccggcggcct cagcgccgct gagcggaccg
     2281 tccgcgtcgt acttgccgaa gcggccggtc aggatctggg gcacgccctt gggcgaggcg
     2341 agcatcttca tacttctcgt atccccccgc tcaagccgtc gccgccgcgc ggctcttagc
     2401 ccgaagcttc tgcgcgaggt cccgcgcctt gtccgcggtc atgcccggct ggcgctggcc
     2461 gtcgagggtc acgagcggct ggccgccgtt gtcgccacca cagtcggcgg cgctgaacct
     2521 cactgacccg tcggcggtga cgccgccgac ttcaacgccc aacgcgtcaa cgagcgcgtc
     2581 gcgcaccctg ccaccgcccg cgaggctgca cgcggcgcaa cggcacagcc cgatctccgc
     2641 cacgcggccc ggctcgaaca cgaggtgcgg ataagacgtc gcgataccgt agatctcgct
     2701 gtaccaggcg ccggtcgagt ggctgatgtg ctgcagcgcg gcgacgggca ggtagccgta
     2761 ggtgctctgc gtctggtcga gcatcgccag gagtcggtgc gggtcgtaat cgttggcggc
     2821 caggatgcgc tcgagcggcc ccaggtcgat tggtagccac gccgcgaacg ccctgtcggc
     2881 gacgggccgg ccggagcgac gcagcgcaga ttcctcgcgc cgctcggcgt actgttcggg
     2941 cgggccccag tggacgtgca tccggttctt ggtgtcgacg ccgcccatgt cgatgatctc
     3001 gatgggacac gccatggcgc actggaagca ggtctcgcac ttgaggttgc ccgcttcgtc
     3061 gtacagcagc accaggcggc cgcggtgatg gggggagatg tcctggcgct gctccgggta
     3121 ctggatcgtg atcttgggct gcacgaagcg cttgaatgtg tagaccatgc ccttggccat
     3181 gccccagccg ggcaccttgc tcatcaggcc catgtctgca ccgccggttg cgtcactgcc
     3241 gagcgagctc ctggacgagc aggatcgcgc ccgcagtcac gaatatgttg agcagcgcga
     3301 tctcgacgag ccatttccag gcgaagccca tcagctggtc gatgcgcacg cgcggcagcg
     3361 tgccgcgcat ccacacgaag atgaacacga aagtgaacgt cttgacgatg aaccagaaga
     3421 gtccgaccac gacctcgaac gacaggaacg cccataccag taccgccgcg atggcgaaga
     3481 cgttgaagag gacgaaggcg acgacgagcg acttcaggaa cgaccagccg ctcttgagca
     3541 tccacagcgg caaggcgacg aggaggacca gcagcgggac gccgatgccc agcacccaca
     3601 gcagccagat gcccatggac gagacatcga tcgccggcgt gagcgagccg atgtggaaga
     3661 agtccaggat tggccgcggg tcgatgggcg cgttccagcc gccgaagaag agcacgaccg
     3721 tcagcgccga gaggatgaac acgttcacgt actcggcgaa gaagaagaag ccgaagcgca
     3781 tgccgctgta ctcggtcgcg aagccggcca cgatctcgct gtcggcctcg gtcaggtcga
     3841 acggggtgcg gttgccctca gcggccgcgg cgatgaaaaa gacgatgaac gcgatcggct
     3901 gctggaagac gtaccaatcc cagaaccagc ccgactgctg gatcacgatc tggttcagcg
     3961 acagcgtgcc ggcgagcatg atgatgccca ccggggcgag cagcagcggc agctcgtagg
     4021 acacgatctg ggccgcggcg cgcagtccgc cgagcaacga gtacttgttg aagctcgacc
     4081 agccggcggt cagcaggccc acgacagtca ggcccgacac ggcgaagaag tacagcagac
     4141 cgatgttcat gtcgaggccc accaggcccg gcgcgaacgg gatgacgagc agcgtcagca
     4201 cgctcgacag gtaggtgatg atcggcgcga cggtgaagac cgccgcgtcg gcaccggtcg
     4261 gcgtgaagtc ttccttcgca agtaccttga agccgtggaa gaccgatgac agcgtgcccc
     4321 acggcccgac gcggttggga ccgatgcggc cggcgaggaa ggcgatgacc ttcatctcca
     4381 tgtagatgat cacgaaggcc accgggatcg tgatgagcag cgtcgccgtc gcggtcacga
     4441 tgaagcggaa aatgtcgatg ttgctgcgca cgagttccgc gagcagctcg atcaccgccg
     4501 ggatcaggcc gagctgccag cccgcgagca gcaggaccgt caggacgacg agcagcagga
     4561 acagcggcag gatgatcttg ccggcgaggg acagccgatt ccacggccgg cttatgacgc
     4621 tcacttatcg accccgccca tgaccggatc gaggctggcc atgatggcca tcgaatcggc
     4681 gatcaggttg cccggcatca ggtggccgac cgcctggagc gtgaagtagg acgggtcgtg
     4741 aatgcgcagc cggaagggct gatcggagcc gtcgctcatg gcgtagcagg agtactggcc
     4801 gcgcgggctc tcgacagaga tgaacgcccg gcccggcggg atgcggatca tgcgcgggat
     4861 cttggccatg atcggcccgt cgggcatctc gtgcagcacc tggtcgatga tgcggatgct
     4921 ctcgcggatc tcgtcgacgc gtagcaggta gcgcgcgtat gagtcaccgg cgtcgcgcgt
     4981 cgggatgtcg aagtcgagct ccggatacac gctgtacggg tgggcgcggc gcacgtcgta
     5041 cggcacgccg gcggcgcgca ggttgggtcc cgtgacgcac agccgcagcg ccgtgtcccg
     5101 atcgagggtg cccatgcccg tcgtgcggcg cacgaagatc tcgttctcgt tgaggaggcg
     5161 cgtctcgttc tcgagctcta cagccgcctt gctcatccag tcgcccaggc gcgacaggaa
     5221 gtcgtggttg aggtcgcggt tgacgccgcc gatgcgcagg tagttgaaga gcatgcgctg
     5281 gccggtgagg ctggcgagca tctccacgat ccggtcgcgc tcgatgaagc cgtacagcag
     5341 cggagtcagg ccgcccaggt cgagcgccag ccagccgatg aacaggccgt ggctggcgat
     5401 gcggttcagc tcccagcaca gcacgcggat gtactcggca cggcgcggga cctgcagatc
     5461 gagcagtttc tcggccgaca gcaccggcat cgcctcgctg aacatggagg ccacgtactc
     5521 gagcgggtcg cagttgctga tggcgtggtg ccagtcgccg ttctcgcaga tcttctcgac
     5581 gccgcggtgc aggtagccca gcaccgggtc aagatccgtg acacgctcgc cgtcgacctt
     5641 gagcacgatg cgcagcacac cgtgcgtgga cggatgttgc ggccccaggt tgagcagcat
     5701 ctcgccgtcg cgcaggttgt agaactcggc ctcgcgctct gtccgcggcg ggtagggcgg
     5761 gaccgcgtcg aagatggtgg cgggcgtctc gacccagatg ttcgggtcgt cgagcgagcg
     5821 gacagagccg cgatcggtgg tcatgactcc tgctccgtcc cggcggcgag ctccgctgcc
     5881 gaaccgggcg tggtcgagcc gagctcggcg cggcgcagga ccggctggcg cagctgctcg
     5941 gcgggcggga atgcctgctc cacatgacgc acgccctcgc cgggcgagcg cgccgcgtcg
     6001 tcgggcagca ggaagtcctt gcgcagcggg aacgacgtgt aatcgggtgg catgtagatg
     6061 cgccgcaggt cgcggttgcc ctcgaagacg atgccgaaca tgtcgtaggc ctcgcgctca
     6121 gcccactccg cgcccttcca gatgaaggtg acgctgggca cgcgcggatc ctcgcgtgac
     6181 aggcctgcgg caatgacgcg cagccagtcg gggctgcgcg gcgaccacag gtggtagacg
     6241 acccacatct ccgtgccggt atcgacgccg gccaggtcgg ccagtagctc gaactcgaac
     6301 tgctcgtcgt cgtggagggt cttcatcacg tccgtgacgt cggcggcgcc gatccagatc
     6361 tcggtcgtgt cacccttctg gatcatgggc gcgctgaagc gctcacccag ggcggcctga
     6421 agtcggcgct tgagctgagt gtccgtaatc gttctcccgg ctacacgccg tcgggaaccg
     6481 tcgggcccac ggcccgctcg gcccaatgac cgcgtcgctc cctgatgagc tgctggagct
     6541 tcatcatccc gtagatcaat ccttccgggc gcggcgggca gcccggcacg tacacgtcga
     6601 ccggcatgac ccggtcaatt ccctcgaccg ttgcgtacga gcgcttgtag cgcccgcccg
     6661 agcacgtgca gtcgcccata gccacgcacc acttgggctc tggcatctgc tcccatagcc
     6721 gcacgagcgg cccggccatc ttggtcgtga gcgtcccggc cacgatgagc acgtcagcct
     6781 ggcgcgggct ggcccggaac gggaacatgt tgaagcggtc catgtcgttc cacggcgttg
     6841 ccgcagacat catctcgatc gcgcagcacg ccagaccgga ggtcagcggc cacaggctgt
     6901 tgagccggat caggtcgagg atgtagtcag ccttggtggg aatgatgtcc agcgcgcgcc
     6961 agcggccggt gtcccccttg tcggcgtgct cctcggcacg caggtgagtg atctgggccg
     7021 gtcgcccgcc gtcataggcg ttcggattga ccggcgtcca gagcctgttc tcgacgatga
     7081 tcggctcgac cacttcgctc ttgtcggcca cggccaacgc gccaggcgtg ttgccgggct
     7141 ggtcagaccc catctcgatc ggcttgttca cttccatgtc aggaccccct tgcgccaggc
     7201 gtagccgagg ccgatcagca ggatgaacac gaacagcagc atggccagga agccgcccag
     7261 caggacgtcg cggaaaacga tcgcccacag gtagatgaag acgacctcga tatcgaaggc
     7321 gacgaacagc agcgcgtaga tgtagaacga cagaccggtt cggccccagg tgtagccgta
     7381 cgtatcaatg cccgattcat acggcatgcc cttgtgccga tcgcccttgg tgcgatggct
     7441 cagcagccac gaggcgatga tcgttatcag cacgaaggtc gtgcccgcga aggcgaaccc
     7501 gatcacgtac cacaggccgg tcgtcaacta ctgcctctcg tcaggcaccg cgttccgcga
     7561 aatgtggttt cgggggctgg ctggagcgga ccgcagctca cgctcattct acgtgtctgt
     7621 ctcggatttg cgacccagac ggcctgccgc ttgagaggcc ctttgcgcct ccttccataa
     7681 cttggacgca ggacagctgc aggaggtgcc agatgcccgc gaaatcagag aaacagcgca
     7741 agttcatggg cgccgagctg gcccggaaac gagccggcaa gaagacccag accggtatga
     7801 gcgagaagaa gctggaggag ttcgccagca agcccggtgg tgggaagaag aagagctaag
     7861 ggccgaccag caggcgtacc cccgggccga ccagggtatg actgccgagc aggatcggtc
     7921 ggggcgagtc ggcggctgaa cgggtcagct cccggagcca cgcgtcgctg acggcggcgt
     7981 gatcgtgcag gtcgctcgcg aaacggtcgg gaggcgttgg ccagcctgac tcgtcgctcc
     8041 ccaggcgggc cgccagcagc acgcgggtgt cgatgatggc ggcgtcggcc agctgcgcga
     8101 tgatctcgga cagggcctca gggccgcgct gctcgagcaa caggctcagg gtcgagctcg
     8161 gcttgcgact cgttgtcttc gccgttccgg ccgccttgcc agcgatcgcc agctgactcg
     8221 atgcccgcag gccgcgctcc tcggccagga atcgcacccg gcagcggaca ttgcgctcca
     8281 gccaggcgag cgtgcgcgac ccgctgcgac caaagacgag cagctccctg agcgggtcgc
     8341 gcaccagggc gcgcaaatcg tccagccgcg gaatcgccag gccggcgtcg tccaccgcgc
     8401 ggcgcagcca cgccggggcc cccggggcga acgcggcgag gcagacgtca agcggcgtat
     8461 ccaggtcgag cgccagccgg tcgcgtcccg tcagctcgtc caccttgaag ccggccaatt
     8521 cctcgagcca gcgcggcagg gcgttgtcgc tcggcagcgc cggcgccttc ttcagcatcg
     8581 acgcgcggcc cagcgccacg atgtcagatg agtagcggtt gttcgtcagc gcctcggcgc
     8641 gcccggacgc cgcgacggcg accaggcgcc gcgcgtcagc caggttgagc cgcgccacgg
     8701 cgccgctgcc caggacgatc acgcctgtgt gcgtcggcgc gaggcgccgg atcagatcgc
     8761 caaaggccag gtcctcgtac cactcgtcaa cgaaggtcgg cggcgccgcg ccggcgcgct
     8821 cgaggagcgc cgcctggcgc cgcacgagct cagcccgcac atcagccagg aatcggacga
     8881 gcggagggtc gccaacagcc gccggacgat ggaagatgag cacggccggg cgggtcattg
     8941 cgcgccgagt ctagatttga aggcacaaac atgaccaagc gtgtgcgggc gaaacgtgtg
     9001 cgtgcttacg tgggcctggg ggcgaacgtt ggtgacacgc gcgccaacct gcgccacgcc
     9061 gttcgcgcca tgacggcgct gcccggcgtc aaggtgcgcg gcgtctcgcg cctgtatcgg
     9121 accaaacccg tcggcgtaac cgatcagcct gacttcctga atgccgtcgc cgcgctcgag
     9181 gttccggccg ggccagatcc ggccactggt gccctggccc tgctgtctgt cctgaaggtg
     9241 ctcgaggcgg caatcggccg caagaagcgc cagcgctggg gaccgcgcga gatcgacctc
     9301 gacctgctgg tgttcggccg tcatcggatc cacgtcgagc gtccccacaa tcaggcagag
     9361 aagcgctggc tcgaggtgcc acacccagag atgcgccggc gcctgttcgt cctggctccg
     9421 ctggctgacc ttgccccatg gctggtcccg ccaggttggg gagagtcggt cgagaccgct
     9481 cgccggcgaa tcgcggcaaa cgagccggag aacgccgttc aggtcgtcgg gaagctgtag
     9541 ttcacgatct ccgggctacc gggcaagacc tccgcgtacg ccgccagccg ctcgttcggg
     9601 ctgcgtgcca gcaccgtgtc gacgaaccac cactcgatgc gcagccgatc gggcgtcagg
     9661 tctgccacca tgtagccgtg gctgtccatg tcgcaccagg cgacgttggg ccgcgcggag
     9721 acgaacagcc gctcgacctc tgttgacctg gtgcgatagc cccagccggt cttgtcgtcg
     9781 aggttctgcg aggtcaggct ggtcgtcacg aactcgggcg cgacccactt gccgggttcc
     9841 tcgtcgcgtt ccagctccag cgccattccg acgtggatat cgccgctcaa caccaccgtg
     9901 ttggcgcgcg ccaggtggcc gatcagcagg tcgcgctcgt atgggtagcc ctcccactgg
     9961 tcgggatcgg gcccggtgcc ggacttgtgg atcagcttga ggtaggtcag accctcgtgc
    10021 agctccggcg gcaggttggg cgcccacgtg cggccgagga tcgacgagtt tgccagcaac
    10081 cgccattgcg ccctggactg ctgcagaccc gcgaagagcc agtcgcgctg cgcgtggccc
    10141 agctggctgc gcgccggagc gaccgcgccg gggccatgga ccggctggtc gcgcaggctg
    10201 cgggtatcga tcacgaacat gtcggccacg tcgcccaggg aaacggagcg gtagacgcgc
    10261 aggggatcgg ccggatcggg caggcgctgg ggcagccact cgcgccgcgc cttgaacgcc
    10321 gccgcgcgcc gcaccggcca cggcccatcg cgctcggcca ggtggttgtc ggcaccgtcg
    10381 cgccagccgc cgtcggcgag ctcgtggtcg tcaatagtgg caatgagcgg gtgcgcagcg
    10441 tgcatggcct gcgtatcggg gtcggcgcgg tactgggcgt agcgtgtccg gtagtcggcc
    10501 agggtcacgc actcgtttcg gggctcgaac ggccggccga tgtccgcccc gggcgtctgg
    10561 ttggcgggcg gcgtctccga cgcctcatag atgtagtcac ccaggtggag cacgaagtcg
    10621 agatcgtcgc gctcggcgat gcgcgcatag ccattgaaga agccggcgtt gaacttcgcg
    10681 cacgacacca ccgcgaaccg gacgcgccgc gcgtcaccca ccggcagcgt cttggtgcga
    10741 ccaacgggcg accattcccc atctggggcg cggaaccgat accagtaggt tgtgcacggc
    10801 ttcagtccac cgacgtcaac agaaacgcag aaatcggagt cgggggtcga ttcgaccgat
    10861 gccgaattaa ctaccgactg gaagtcggag ccttcggcca cctcccatcg caggctcccg
    10921 cctctttcga gccgagtcca aaggacaact cgatcgggcg cggggtcgcc gctcgcgacg
    10981 ccgtagcgga actggctcaa gtcgagacgc tagagacgga cggcgggggg cttgtcgcgg
    11041 tcgaacatgt gcaccgtgtc gatgaagcgc acttccttgc tctggtagcc catcaccagc
    11101 gaatgcgtcc gcgcgccgcc accaaagaag cgcacgccgc ccaggaggtc gccgtcggtc
    11161 actccggtcg cggcgaacac gaggttggtg cccggcgcga gatcggaggt ggtgtagaca
    11221 cgctccatgg acgggtcgac gcccatcgcc accgcgcgat cgcgctcctc atcgctgcgc
    11281 cagcgcaacc gagcctggat ctcgccgccc aggcagcgca gcgccacggc cgataggacg
    11341 ccttcgggcg cgccacccgt gcccatgacc gcgtgcacac ccgtgccgcg caccgcacat
    11401 gagatcgcgg ccgacaggtc gccgtcgctg atgagcttga tgcgcgcgcc ggccgcgcgc
    11461 acttcgtcga tgagctgggc gtggcgcggt cggtcgagta tgacaaccgt caaatcggcc
    11521 actttgcggc cgaggatatt cgcgatccgg tcgaggttct cggtcggtgt gaggcggatg
    11581 tccaccttgc cggccacgtc gggccccacg cacagcttct cgagataggt atcgggcgcg
    11641 ttgagcaggc cgcccttctc cgacgcggcg atgaccgtga tcgcgtttga gttgccatgg
    11701 gcgacgaggt tcgtgccctc gagcggatcg accgcgatgt cgatctccgg ctggccaggc
    11761 atgctccggc cgacgctctc gccgatgtac agcatgggcg ccttatcgcg ctcgccctcg
    11821 ccgatcacga ttgtgcccgc caggtcgaca tcgtcgaacg tcttgcgcat tgcctcgaca
    11881 gcggcgtggt cggcggcctg gttgtcgccg cgacccatgt ggcgcgccga tgcaatcgcc
    11941 gcggcctcgg tcacgcgcac catctcgagc gcgagcagcc cttccatagc tccccctagt
    12001 ggcggtagtg gcgcttgccg gtgaacacca tcgccagatg gtgacggttg gcgacttcga
    12061 tggccatctc atcgcggacc gagccgccgg gctggatgat cgcggtcacg ccccgctcag
    12121 ccgcgagctg gatcgcgtcg gcaaacggga agtacgcgtc tgacgcgagc actgccagct
    12181 tggagcgctc ggccgccttg tgaagcgcga tctcgaccgc ggtcaggcgg ctcgcctggc
    12241 ccgcgcccac gccgacgagc gcggtgtgat gggcgagaac gaccgcattg ctcgtgacgt
    12301 ggcgcaccgc gcgccaggcg aacagcaggt cggtgagctc ctcgagcgtc ggccggcgct
    12361 gggtcacgac cttgagctgc gaccgatcga cctctgacga gtcacgtgtc tcgacgagca
    12421 gccccccgtc aatgcgcgcg aaatcgagcc gggcgatgcc gtagtcggac agcccctcgg
    12481 tgaagcgatg aggcacggcc aacagcgcca ggcgctgctt ctccgcgagg atctcgcgcg
    12541 ccggcgccgt aaaccccggc gcgacgatcg cctcgtacgc gttgccggcg agtgcctcgg
    12601 cggtgttgcc gtcgacctcg cggttgagcg ccacgacagc gccgtatgcc gacaccgggt
    12661 cgccctccag cgccctctgg taggcgtgca cgagcagctc gtcggacgca agaccggtgg
    12721 ggttgttctg cttcacgatg cagcaggtcg gggcggtgaa atcggagacg atccggtacg
    12781 ccgcgtcgag atcgatgaga tcgttgaagg tcggctggtc gccctgcagc tggtgggcat
    12841 cggcaagcgc gcctgatcgg tgcatcgatt cgcggtagaa cgccgcgtgc tgctgcgggt
    12901 tctcgccgta gcccaggtcg cgcaccttct cgaccaccac ggccagccga ttcggaaagc
    12961 ggttgccacc gagatgattg agatagccgg cgacctccgc gaagtaggcc gacacatctg
    13021 cgagggcgtc cgcagcgagg tgctgccgaa actcggctga cacgatattg cgcgtgcgca
    13081 gctcttggag aagctggggg taatggcgcg cgccggacac gatcgccacg ctcgcgaggt
    13141 tgcgcgcggc ggccgccatg agcgacgtgc gcgtgacatc gatcatggcg atcgcctcgt
    13201 ccagcggcac gacgcgctcg cccacctgtg gcgcaaacgg cttgatgttg gcgacgacga
    13261 tgtcgaagcc ctcggccgat gcccccgcct tctccaccgg ctgcgcgctt acggctgcct
    13321 gggccagaac ggcaagcgcg tcggcgtcgg catggagctc gacgccaagt gccgccagat
    13381 cgcgcgccag atccgcgacc ccgtcactgt tggcgaccga gagcagggcc cgcacgtctc
    13441 ttttgagtcc ctctccggtg ggcgcccgcg gctccgttgt catggagcac cggggcaaca
    13501 acgggccgag tataagtgag gcgctatcct gcggctgccc tggacccaag cggcagcagg
    13561 agttccccaa acgtgaaagt cagtctcctc gaggacctct tctggtacct gaacaagccc
    13621 tttgtcggtg gccagccaga atcgggcggt ttccctgaca tcttccatcc catctggatt
    13681 gccgccgcgg tcatgctcgt cctcacggtg gtgctgtaca acgtgcgggt acgccagctg
    13741 caccgccacg agcccctgcg ttcgatgcag gaatggctcc tgtggaccgg gctcgtcacg
    13801 tttgggctgg ttatcgtcgg cacggtcttc cggttctact tcctgttcgt cctgctcttc
    13861 atcgtgatcg gcctcgcggc attcgtgtgg atccgcttcg tccacttccc gccatacatc
    13921 gaggcgtaca acgagcagct ccgccgcgcg cgcttcttca gccagagcaa gtacaaggcg
    13981 gccgagtcga ctgttcgcgt caaacgcaac cgcggtgggc gccggcggcg ctagccgcat
    14041 cccaagccga gcgggcggag cgcggcctgg gccgtgtgcc aaccgcgata gcgcgcagca
    14101 gcggcccgac ggccgccctg cggaggtgaa ttgagtggag attcgccgct tcgggccggg
    14161 tcatcgccgg gcggagggtc cgccgggcac caagggcgtc gccggcgccc tgctgctttc
    14221 ggatgaccgg gcagtcatca ccgaggtcgc ctttggtccg tacgcgatga tccagcccca
    14281 cgcgaaccag tcgaacaccg cgctgttcat cgtcatcagc ggcggcggct tcgtacaggt
    14341 gggcgaggag cgtgcccggg taagtcacgg cgaggccgtc atctggccgc ccggcatcct
    14401 ccacggcgca tacaccgacg ggacggagat gcgggccatc atcgtcgagt tggtcggcga
    14461 cgggaccgcc gtggtcgagg gccgggctca tgccgccgag tcggccgcga ccaaggccaa
    14521 gggcgcgctc gccgagcggc agcggacgcg cgacgagtac gactcgtccg agggcgaacc
    14581 ctggtgagct cggttgggcg ggtgccgtga tgcccctgtg acgtcaaacg tccgcctacc
    14641 cgccgtggac cccgtcggtt tgtaccagcc cccgcccgtg atggctggtc ggcgggcggc
    14701 ttgagggtga aagcgaccaa aaaggctgca gatcgctttc tcgcgggtgg gcgggccgcc
    14761 ggcgccggac gtttgacgca cctggatgat caagatggcc gtgtcgccgc gatcgacgtc
    14821 cagccgctca ccactgagcg gttcgcggac ctggcggagc tgttcaacga gggcggcgac
    14881 cccaagtggt gctggtgcca gtacttccgc aaggcgggtc tcgactggac caactcgaca
    14941 accgcaggca accgggccgc gctcgagtcg ctggctgcgc ggcccgacct cgcgccgggc
    15001 ctcgttggct accgcgacgg ccgtgttgtc ggctgggtca gcctcgcgcc gcgcgaggac
    15061 tacggccggc tcgagaacgc caaggtcctc gctcgtgtcg acgacacgcc ggtctggtcg
    15121 atcgtctgct tcgtcgtatc gaggaaggcg cgcggccagg gcgtggcaac ggccttgctg
    15181 gacggcgcga ttgcctatgc cagggaacat cgcgcgacga tgctcgaggc atatccggtg
    15241 gccgcgacac gcggtcgcat cagtccggcg agcgccttcc acggcgtcca gtcgatgtat
    15301 gagaaggccg gcttcgcggt cgtcgcgacc cgtcagtgga acgagacctc gcccatccgg
    15361 ccgatcatgc ggctggcact gggagcgcaa gggaggaagc ggtgaagctc ttcgcgacga
    15421 tgatgctctc cgtcgacggc gtctatcaag gcccgggcgg cccagacgag gaccggcgcg
    15481 gcggcttcga gcgcggcggc tggactgccg cgtccgcgtc cgaggagatg ggcccctacc
    15541 tgaccgagat gttcgagcgc gcagatgcgc tcttgctggg ccgccagacc tgggagatct
    15601 gggagccgta ctggcccaag cacgacgaca acccgttcgg tcacatgatc aacgtcctgc
    15661 ccaagttcgt gacctcgacc acgctgaagg acccgacgtg gcagaacact catgtgctgt
    15721 cgggcgacgt ggaagcgcag atccgcgaac tgaagggacg gccggggcgc gagatccagg
    15781 tccacggcag cggcgtgctg ctgcgctggc tgctcgagcg cgacctcgtc gacgagctga
    15841 acctgcgcat ctcgccggtc atcgtcggcg acggtctgcg cctcttcccg gaaagagggc
    15901 cgacgcacaa cctggagttg gtcgactcgt ggacgaccaa gacgggcgtt ctggtccaga
    15961 cctaccgccc cgctggccgc gccaccttcg gtcctatcgg ctagccggca gccggctagg
    16021 agatcgggat ctttttctcc gggtcgacga agggcatcgg cacgacggtc gcgtggcggt
    16081 cgccccactc ggacgcgacg cgcaacgtgg agcccggcgc ggacaggtca accggcaccc
    16141 agcagaagcc gatgttcctc ttgaggcgcg gcgagtagat cgccgaggac acctgtccaa
    16201 cggcgtctga cccgccgtcg cggaacgcat cccacttgtt gaagttgagt gctgggaatg
    16261 ggtcgccgtc catctcgacg ccgacgatct tgcgcgtaac gcccgccgcc ttgatgcgcc
    16321 gcaacgcggc catcgagatc gaggccgaat cgggcagctc ccactggatg agccgctcca
    16381 gccccatctc gagcgggttg ttgtcgtagt tcatgtcggc gccccagttg aagatcgcgc
    16441 cctcgatgcg ccggatatcg ctgggtccgg tcggcttcac gccgtagggc ttgccggctt
    16501 ccatgaccgc ctgccagagc tgcgtgcctt tcgatcggtc gagcaggtag atctcgtagc
    16561 ccacctccga cgtccagccg gtgcgggtga taacgaccgg gatgccgttg acttcggctc
    16621 gggtccagaa gtagtacttc atgtcgtcga ccacatcgcc gacgaggtcg tgcatcaggt
    16681 tcttggactt ggggccctgg atctgcatgg gcgcgacgtc gacttcgcgg atcgtcacgt
    16741 cgaggtcggg ataggcgtgg cgcaggcctc ttgcgaagag cagagcgtcg gacgacgcga
    16801 gggcgaacca gaaggtgttc tcgtccatcc gcgtcaggac ggggtcattg atgatgccgc
    16861 cgtcgccatc gcagatgagc acgtacttgg cctggcccac tgcgcacttg ctcagatccc
    16921 ttggcgtgag gagctgggcg aacctgaaac catccgggcc gctgatctcg agacagcgct
    16981 cgaccgccac gtcccacacg gtgacgtcgt tgagcagcgt ctcgaactcc tcctcgaacg
    17041 actcgaagcg gatcgggaag agcatgtggt tgtacacggt gaagcccttg ggtgaatacg
    17101 cctgcgtggc ctcgtaatag gggctgtgcc gcagccgcgc gccgcgctgc accaggctca
    17161 tgtcgaactc ggccaccgac tacctccgct cagctgcctg cccaaggaat gcccccagtc
    17221 gcccaagcat catcgccgtc gcgccccaca cgaggtgctc gccgacgcgg tacgcaccat
    17281 agcgaagccg gtagccatcg cgctcgtcag tgatgatctc gatgggcgcg cccttcacga
    17341 aggcggccag cggagcgccg aacacgctcg cgacctcgta accatccggc gtgagggtgg
    17401 gcggccgctg cgcgaacgcc acgaccggcg cgaccatgaa gccgctcacg cgcacgtcgg
    17461 ccagcggcag cacgccggcg atggtcacgc ccgcctcgac gggatcgaga cccacctcct
    17521 cggccgcttc ccgcagggcc gccgcggccg gcgtcgggtc cgcggcatcg atggcgccgc
    17581 cgggcaggct gatctggccg gcgtgccggt gatcgcccgg cgaccgctcg gtcaggatga
    17641 ggtacgcgtc gccgttggcg tcggggtaga tgaggatcaa cacggcagcc tcgcgccgcg
    17701 gaccgtccgg gaagcgcggg gcaccgccgg gcacgccctc cggatcagcc actacgaccg
    17761 gcatcagctc cggcggcgga ggcggcagcc gatcaggcac ctgcgccagc cgcgcctgcg
    17821 cttcctcgaa cttcatcagc ccgcgagcgc cggggcgtcc cgcccgagga tctccacggc
    17881 gaacccgccc atgccctgcg gatcgagcag ccgcgcgacg gcggcgcgca gcgtgagcgc
    17941 ggagtcccag tcggcttcag cggcgtcgcg cagcgcgcga tacacctcgt cgagcccatt
    18001 gccgagcaga aactgggcct ggcgcgtccg ggcgatgacc tccaggccca cggcccgtgc
    18061 cccatcctcg agtgcgtcga ggtccacgtg ggcggtgatg tcctggtggc cgaccgagcc
    18121 aaggacgtcg ctcgagacgt gctggccacg aaaagcgcgc attgtgccag tcgcacgatc
    18181 cgggccgtac aggtccgccg ccgccaagcc gtaatcgaag atcagcacgt agccccgctc
    18241 caggtcgcgg ctcacctcgg ccagccagtc gagcatggcc aggttgacct ccgcgcgttg
    18301 cccatcggcg agcctgattt gacggccatc gaaccacccc gcgaggcgcg ggtctgagag
    18361 ctccgtctcg acttcgacga gcctgccttc ggaccggccg actctgagct cgcgcagtcc
    18421 gtcctcccga gccaccacgc gatggaccgg caacgcgtcg aggaactcgt tggccagcat
    18481 gacgccggtg agcctgctgg ctggccgcgg ctgatcgaat tcgatcggct cgtagctcac
    18541 cccggggggc agcgcctgac ccagggtgcc gcggccggcg ccgtactcgc gcacggtgaa
    18601 atcgatcggg cgaccgagct gttggcgcat ccgctcgatc tggcgcacga ccgtttggcc
    18661 gaagatcggg tgcagctccg gcgccgtcag gaagtcgccg gaacgcgtcg tccgctcgcg
    18721 gctcctggcg tagtaaccga ggcccggttc gtacagggcg cgctccatga accgcgcgaa
    18781 ggtgatgggg ccgcgctcgt cgatctcggc caggatcgcc tcgagcagcc gcggctcgcc
    18841 tggatcgtcg gccggtggcg ccgccaggga cgaccggacg ggttcagctc gcacttcgca
    18901 cccagaatat gcggtggcta cgatcaagcc atgacgcaca tcgagcacca ctcgctcccg
    18961 gctggactgg cgccagccac tggctacagc cacgcagtct ctggcaccgg ccggctggtt
    19021 gccatcgccg gccaggttgc gatggacgag aacggcgagc tcgtcgggcc gggtgatcca
    19081 caggcccagg ccgagcgcgt ctttgccaat ctggccctgg ccctcgaagc ggcgggcgcc
    19141 acgttcagcg atgtcatcaa attcggcgtt ttcgtaaccg acatagcgat cctgcccgtg
    19201 gtgcgtgagg tccggaaccg ctacttggac accgctgggc caccggccag cacggcagtg
    19261 caggtcagcg ccctgttccg gcccgggtac ctgctggagg tcgaggcgct ggccgtggta
    19321 ccggttgaca agtgaccctc gatcgcaagc tggccgatcg cgcgctgtgc tacctcgaga
    19381 cgatcggccg cgtcaccggg cggccgcacg tcatcgagat gtggttcgcg cctgaccctg
    19441 cgcgtgatcg cctgtacatg atggccggcg gacgcgatga ggccgactgg gtgcgcaacg
    19501 tgcgccacga cggccgggtg cgcatccggc tgggcaagac ctggctcagc ggccgcgcgg
    19561 ccgtaatcga gggcacggcc gacgagccga tggtgcgacg cttgctcgcc gcgaaatacc
    19621 agggctggac cgacggcagg ccgctcagcc attgggcgca gaccgcgctg ccggtcgcca
    19681 tggagttgga cggctagttc agccgctccg tcgcgccgcc ggccggcgcc gcttcgggct
    19741 ccggctcccg ctgcgccgcg cgacgcacgt cgcgccgctc atctgtcgcg ttggcgacga
    19801 ctgtctcgcc gcctgacgtg aagagcagcg tgccggaccc tgcgtccgcg tcgaccttga
    19861 tggtcgtgcc cggcttgaac ttgccctcga gcagcgcccg tgcaaggggg ttctcgacca
    19921 gccgctggat ggagcgcttc aggggccgcg cgccgtacac cgggtcgtag ccctcggccg
    19981 cgatcagctg gcgcgcggcg ggtgtcactt ccagcgtcag gtcgtgctcg gtcagccggc
    20041 gcgtgaggtc ggccagcagg agctcgacga tgtgcgcgag gtcgtcctcc gtcagggcgt
    20101 ggaacacgat gatctcgtcg atccggttca ggaactccgg ccggaacacc tggcgcagcg
    20161 tgtcggtcac ctgcgtcttc atctgctcgt acgactcttc gtcgcgcctg cccgcgaagc
    20221 cggtgatgaa ctggctgccg acgttgctgg tcatgacgat gaccgtgttc ttgaagtcga
    20281 ccgtgcgccc ctgaccgtcc gtcaggcggc cgtcgtcgag cacctgcaac agcacgttga
    20341 acacgtcctg gtgcgccttt tcgatctcgt cgagcagcac cacctggtac ggccggcggc
    20401 gcacggcctc cgtcagctgg ccgccctcgt cgtagccgac atagccgggc ggcgcgccga
    20461 tcaggcgcga gacggagaac ttctccatgt actcgctcat gtcgatccgg gtcagcgcgg
    20521 cctcgtcatc gaacaggaag ttcgcgagtg cccgtgcgag ctcggtcttg cccacgcccg
    20581 tggggcccag gaagatgaac gagccgatcg gacgcttggg atccttgagc cccgcccgtg
    20641 cgcggcgtac ggcgtctgag acggcggcca ccgcttcgtc ctggccgacg acgcgcttgt
    20701 gcagctcgtc ctccatgcgg atcagcttgg cctgctcgcc ttcaaggagc cgcgtgaccg
    20761 gcacgccggt ccacgcagaa acgatgcgcg cgatgtcgtc ggcatcgacc tcttctttca
    20821 gcaggcgctg ggcgcccgac tgggccaggg ctgcctcctc atcggccagg cgcttctgag
    20881 cctccggcag cttgccgtac ttgagctcgg cggccttgcc gtaatcggcg gcgcgctccg
    20941 ccttgtcgat ctcgagttgc aggtcctcga gctgcttctt ggtcgcgcgc aggtcggcga
    21001 tcttggcctt ctcctgctcc cactggctct tgagcgcacc ggcacgctcg ccgatgtcgg
    21061 ccagttcctt ctcgagcgcc ttgagccgcg ccttggatgc gtcgtcgcgc tccttgcgca
    21121 gcgcctcgcg ctcgatctcg agctggatgc gccggcgctc gagctcgtca agttcgacgg
    21181 gcatcgaatc catctccatg cgcaggcggc tggctgcctc gtcgacgagg tcgatcgcct
    21241 tgtcgggcag gaagcgctcg ctgatgtagc ggttggatag gacggcggcg gctaccagcg
    21301 ctgaatcggt gattcgcacg ccgtggtggt tctcatagcg ctcgcgcagg ccgcgcagga
    21361 tcgagatcga ttcctcgacg ctcggctggt cgacgtagac cggctggaaa cgacgctcca
    21421 gcgccgcgtc cttctcgatg tgcttgcggt actcgtcgag ggtcgtggca ccaatcgtgt
    21481 gcagctcgcc tcgggcaagc atgggcttga gcaggttgga cgcgtccatt gcgccctcgg
    21541 ccgcgcctgc gccgacgacc gtgtgcagct cgtcgatgaa cagcacgacc tggccttccg
    21601 agtccttgat ctccttgagc acggccttga gccgctcctc gaactcgccg cggtacttgg
    21661 cgccggcaat cagcgcgccc aggtcgagcg cgacgacgcg cttgtccttg agcccctcgg
    21721 gcacgtcgcc gcgcacgatg cgctgcgcga gaccttccgc gatagccgtt ttgccgacgc
    21781 ccggctcgcc gatcagcacc gggttgttct tggtgcgccg gctcaggacc tggatgacgc
    21841 gtcggatctc ctcgtcgcgg cccaccaccg ggtcgagctt gccctcgcgc gcgtcgcgcg
    21901 tcaggtcgcg gccgtacttc tcgagcgcct ggtacgtgcc ctctggattg gcggaagtca
    21961 cgcgctggcc gccgcgcacc ttctgcagcg cgcccagaat ctgttcgtgc cccgcgccgg
    22021 cggcctcgag gatgcgctgc gcgtcgcccc ctgactgggc ggccgcgagc agcaggtgct
    22081 ccgtcgagac gtattcgtcg tggaggcgct gcgcctcctc ctccgcgcgg cgaatgagct
    22141 cctgcgcgcg cggatccagg ctcatcgagc cgccggccac cttcgcgcgg tggctcagcg
    22201 ccgcggcgat ctcgacaccc acctgcgcgg ggtcggcgcc cagggcgcgc agagttgccg
    22261 cggcaattcc ctccgcgtcg tcgagcagcg cggcaaggat gtgctccgcg tcaagcacgg
    22321 gcgagtcagc gtcggtggcc aggcgctgcg cggcgacgat cgcctgctgc gccttttcgg
    22381 tgtatcggtc gagcttcact tcttgtgtcc ttcggctaga tccaggaatc tctcggctgc
    22441 gcgcttggcc tcttccgata gaccgctcgg gagaacgacc cgcaccttca cgaacaggtc
    22501 gccccggcct tcctttcgga aatgaggcag accctggccc gccagtcgaa atgttcggcc
    22561 ggtctgggtt tcgggcggaa tgcgcagcat caccttgccc gtgagtgttt tgattgcggt
    22621 ttcgcccccg agcagcgcct cgcgcagcgt gatgggcact tcgcgcgtca ggttggcgcc
    22681 gtcgcgcgtg aacaccggat gaggcgccac gctcaccttg acgtaagcgt cggcacctgc
    22741 cgcgacgccc gaaaagcgaa tgcgctggcc gttggccacg ccctgcggga tcgtcacctc
    22801 gatgcgcttg ccgtcgacat cgagcaatcg cttcgtgccg ctagccactt cctcgagggt
    22861 cacctcggcc tgcgcctcca gccgcgccgg caccgccgcg cggccagtgc cgttgccgcc
    22921 caggttcatg ccagccagca gatcctcgat ggactgaccg ccgcgcgtcc tggccttggt
    22981 acgcgtccgc ccgccgccgc ccgactctgc ctcgaacccg ccgccgccga agaacgtgcg
    23041 gaagaagtcg ctgaagcccg cgagatcctc ggcgttgccg cggtactcga agcgcactcc
    23101 accgggcgcg ccgccgccaa agccgccgaa gcccgcgaac gggttaccgc cgccggcacc
    23161 ggagccgcca gcctgctggt acgcgcccca gttgctgccc agctggtcgt agagcttgcg
    23221 cttctcggga tcagagagga cctcgttggc ctcgttgatc tccttgaagc ggctctccgc
    23281 cgcggccttg cccttgttcg cgtccggatg gtgctgccgg gcaagcttgc ggaaggcctt
    23341 cttgatgtcg gcctggctgg cggtgcgtgg cacccccagg gtcgcgtagt aatcccggta
    23401 ttccatcgtc aatcaccctg gaatgggttc ggtcgaagcc ccgctcgcac gacgctccga
    23461 cctccgccgt tagtgagtgc tcgcgatggc cggtggctgg tcctggccgg ggaagccgcg
    23521 gcgcatcttg tccggtgcgg ccggcgactc ctcgtcgacc ggctcaccct gctcgaccac
    23581 ctcggccggc ttggcctcaa gcgccttggg cgcgcgccga agcgcgtgct ccagcacctg
    23641 gtccatcgac tcgaccatga cgagcttcat ctgcttgcga atctcgtcgg gcacgtcgag
    23701 cagatccttc tggttcttga gcggcaggat cacctcccgc gcacccgcca ggtgtgcggc
    23761 caggatcttg ctcttcaagc caccgatcgg caacactcgc ccacgcagcg tgatctcacc
    23821 ggtcatggcc acgtccttgc ggacggggat gcccgtgagg gcgctgacca tcgccagggc
    23881 catcgtgatg ccggccgacg ggccgtcctt gggaatgccg cccgccggga catggatgtg
    23941 caacgtcttc ttctcgaaga cctcgcgcgc gatgcccagc tgcgaggcgt gggcgcggat
    24001 ccaggacagg ccggcgcgag cggactcacg catgacctcg cccagctggc cggtgaggat
    24061 gaagtcctcc ttgccgtcca tcgtcgtcac ctcgatcgac agcacgtcgc cgccgacctc
    24121 cgtcaccacg agacccgtgg ccgcaccggt ctggtcttcc ttctcgagct cgccgtactc
    24181 gtagcgcggt gggccgagca gctcgttgag cttcttgaca tcgaccgtga ccttgcgctt
    24241 gcgtccctcg accacctggc gggcgagctt acgcatgatg ttggcgatct cgcgctccag
    24301 gttgcgcacg ccggcctctt tggtaaaggc ctgcaccagc tcgaccattg cctcatccgt
    24361 gaactcgacg ttcttgggcg tcaggccgtg gttctcgagc tgcttgggga tcaagaagcg
    24421 cttgccgatc tcgatcttct cctgctgcgt gtagcccggc agctggatga tctccatgcg
    24481 gtcgcgcaaa gccggcggga tgggatcgag caggttgccc gtcgcgatga agagcacctt
    24541 cgacagatcg aacggcacct cgaggtagtt gtcctggaag gtgttgttct gctcgggatc
    24601 gagcacctcg agcagggccg agctcggatc gccgcggaag tccatgccga tcttgtcgac
    24661 ctcgtcgagc atgaacacgg ggttgttcgt gcccgcggtc ttcacgttct ggatgatccg
    24721 gccgggcagc gcgccgatat aggtgcgccg gtggccgcgg atctccgcct cgtcgtggat
    24781 gccgcccagg ctcatgcgca cgaacttgcg acccatggcg cgggcgacgg acttgccgag
    24841 tgacgtcttg ccgacgccgg gcgggccgac gaaggcgagg atgggactgc ggatcttgtc
    24901 cgccaggctg cgcaccgcga ggtactcgag gatgcgctcc ttgatcttct cgagaccgaa
    24961 gtggtcctca tcgaggatct tggccgcctc cttcagatcg agccgatcgt cggtggtgac
    25021 gttccagggc aggctgatca gccagtcgac ataggtccgg atcacgccca cctcgggtga
    25081 ggcagacggg atacggctca tgcgatcgac ttccttgatc gcgcgcgcct tgatctcctc
    25141 gggcatgccg gcctgttcga ccttctcgcg cagctcggcc acctcggcct gctgcggatc
    25201 gtcctcgccc agctcgcgct ggatggcctt cagctgctcg cgcaggatgt actcgcgctg
    25261 ggtcttgtcc atctcggact tgacctcgga ctggatcttg cccttcagct cgaggatttc
    25321 gatctggcgg cccaggaagt tggagacgag cttcaggcgc tcaaagacgt cgaaggtctc
    25381 cagcagctcc tggcgctgct cggtgctcat gtccgggctg taggcaatca tgtcggccag
    25441 caggcccggc tcggtgatgt tgcgtgcagc gacggccgcc tccggcggga cgggagcgcc
    25501 cgactggacg tattgctcga tctggccctg gacggtgcgc accagcgcct gaacttccag
    25561 cccgctcgcc ggctgctcct cggccatctc gacgcgcgcc aggatgtacg gatccgcgtt
    25621 gacgaagccg agcagcttga tgcgctgctg gccctgcacg atggcacgca cggtgccgtc
    25681 ctggagctgg acgacctggg cgatcttggc cagggtgccg acctcgtaga gctcggaggg
    25741 atcgttgatg tcctcgcggt cggcctggcg ctgcgtgacg agggcgatcg gggttgagtc
    25801 ggcggtcgcc ttgttgagcg cctgcaccga cttgtcgcgg ccgacctgga gcggcacgat
    25861 catctccggg aagatgacc
//
LOCUS       DATBRX010000164         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_285520, whole genome shotgun sequence.
ACCESSION   DATBRX010000164 DATBRX010000000
VERSION     DATBRX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285520"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /gene="dnaE"
                     /locus_tag="VIF08_06295"
     CDS             complement(<1..1532)
                     /gene="dnaE"
                     /locus_tag="VIF08_06295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8135634.1"
                     /translation="MAVAPNDFTHLHVHSEFSLLDGLGRISEMVDQAKKLGFDSLGLT
                     DHGALYGAVAFYQACRTAEIKPIIGVETYVARRSMADREGKADAQPYHLILLAKDWTG
                     YQNLCRIVTDAHIDGYYYKPRIDRDYLAKHSAGLVGLSACLNGEVARAIETGDMDEAR
                     RIAGSYRDILGDGNFFFELQDHGLPEQRKLNEQLIKLGPEMGIPLVATNDLHYVRREQ
                     YDAHDVLLCVGTAANLDTPGRMRFESSDFYLKSAAEMAALFSDQPDAIANTRRIAEMV
                     DLQLPFGQLRLPDFPVPEGHTVESWLRIECERGLRERYGTVTEPLQKRLDYELGVIIS
                     MGYAGYFLIVADFVRFAREQGIATTCRGSAPGSIVTYTLGITPVDPIHYELPFERFLN
                     PDRVTMPDIDVDFEDARRDEVIRYVTQKYGSDHVAQIITFGTMLARAAIRDVGRVLGL
                     GYGEVDRIAKAVPNVLGIKLEEALETSPPLREMRDADPKVKRLLELAGQLEGVARNAS
                     THA"
     gene            complement(1719..2477)
                     /locus_tag="VIF08_06300"
     CDS             complement(1719..2477)
                     /locus_tag="VIF08_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8135635.1"
                     /translation="MNPSSLPAPDPPGRLKRWFIRYATRLVFGTYLKVRYEYLAPLPE
                     PPYVLNFNHPGWVDPFLLVGWWPRRHRIYVFGPKEEDMSVGWRNHVINWSHMAVPFKP
                     SRSDLVDATKRAQGVIEAGFVLGIAGEGRLSDREGEIVPLQEGVAFFALRAQTPIVPI
                     GIIGTRWVKLRKLVRVRVGKTIYLNGRKANRAGVDSLKAELTDAMNALLADAPQDPPP
                     GRTGRWLTDLFADRPWLNEKKPEEPPTDTDDKPA"
     gene            complement(2449..>3013)
                     /locus_tag="VIF08_06305"
     CDS             complement(2449..>3013)
                     /locus_tag="VIF08_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8135636.1"
                     /translation="HHCRWLAHHAARVGANVGLRGEELEAVAYGALLHDVGKIGVSEH
                     LLRKEGPLSADEWLVMRRHPEIGEMIIRPLRASRVFAPIIRHHHERFNGTGYPDGLRG
                     ESIPLGARIVAIADAYEAMVHGRPYQPSQTHERAADELRRLRGAQFDPDLVPVFLDEL
                     ERDTLGVPPLVALPPVARLEPEFAAGA"
BASE COUNT          485 a          977 c         1041 g          510 t
ORIGIN      
        1 gcgtgcgtcg aggcgttacg ggcgacgccc tcgagttgcc cggcaagctc caacaggcgc
       61 ttgaccttgg ggtcggcgtc gcgcatctcg cgcagcggcg gtgaggtctc gagcgcctcc
      121 tcgagcttga tgccaagaac gttgggcacg gccttggcga tgcgatcgac ctcgccgtaa
      181 cccaggccca gcacgcggcc gacgtcgcgg attgcggcgc gggcgagcat cgtgccgaag
      241 gtgatgatct gggccacgtg gtcgctgccg tacttctgcg tcacgtagcg gatgacctcg
      301 tcacgccgcg cgtcctcgaa gtcgacatcg atgtcgggca tggtcacgcg gtcgggattg
      361 aggaagcgct cgaacggcag ctcatagtgg atggggtcga ccggcgtgat gcccagcgtg
      421 taggtcacga tggagcccgg cgcgctgccg cggcaggtcg tcgcgatgcc ctgctcgcgc
      481 gcgaagcgca cgaagtcggc gacgatcagg aagtacccgg cgtagcccat cgagatgatc
      541 acgcccagtt cgtagtcgag ccgcttctgg agcggctcgg tcaccgtccc gtagcgctcg
      601 cgcaggccgc gctcgcactc gatgcgcagc caggattcga ccgtgtggcc ttccgggacg
      661 gggaaatcgg gcagacggag ctgaccaaat ggcagctgca ggtcaaccat ctcggcgatg
      721 cgccgcgtgt tggcgatggc gtcaggctga tcgctgaaca acgccgccat ctcagccgcg
      781 ctcttcaggt agaagtcgct cgactcgaag cgcatgcgcc ccggggtatc gaggttggcc
      841 gcggtgccca cacacaggag cacgtcatgc gcgtcgtact gctcgcgccg cacgtagtgc
      901 aggtcgttgg tggccacgag cggaatgccc atctccgggc cgagcttgat gagctgctcg
      961 ttgagcttgc gctgctcggg caacccgtgg tcctgcagct cgaagaagaa gttgccatcg
     1021 cccaggatgt cgcggtagct gcctgcgata cgcctcgcct catccatgtc gccggtctcg
     1081 atcgcgcgcg cgacctcacc gttcaggcac gcggagagac cgacgaggcc ggctgagtgc
     1141 ttggccaggt aatcgcgatc gatgcgcggc ttgtagtagt agccgtcgat gtgcgcgtcg
     1201 gtgacgatac ggcacaggtt ctggtaaccg gtccaatcct tggccaggag gatgaggtgg
     1261 taaggctgcg cgtcggcctt gccctcccga tcggccatgg agcgccgggc gacgtacgtt
     1321 tccacgccga tgatcggctt gatctccgcc gtgcggcagg cctggtagaa cgcgacggca
     1381 ccgtagaggg cgccgtggtc cgtcaagccc agcgagtcga agccgagctt cttggcctgg
     1441 tcgaccatct cgctgatccg gcccaggccg tcgagcaggc tgaattcgga atggacgtgg
     1501 agatgcgtga aatcgttggg tgcgacggcc aattaccttc ctccgcagtc tgccttcggc
     1561 atctgctgcg cgcgaagcgg tcgggctccc gcgaaccgcc gggtccgccc gtcgcttgct
     1621 ccgaccttac tttccgcgcg aaggcggggc taccgggtca tcaacgaact tcgtccacaa
     1681 gtcatccagt atagaacggc cgttcggtac tgtcagcgct aggcgggctt gtcatccgtg
     1741 tcggtcgggg gttcttctgg cttcttctcg ttgagccagg ggcggtccgc gaacaggtcg
     1801 gtcagccagc ggcccgtgcg gccgggtggt gggtcctgcg gcgcgtcagc gaggagtgcg
     1861 ttcatggcgt cggtcagctc ggccttcagg gaatcgacgc cggcacggtt cgccttgcgg
     1921 ccgttcaaat agatggtctt gccgacgcgg acccgtacga gcttgcgcaa cttgacccag
     1981 cgcgtgccga tgatgccaat gggcactatc ggggtctggg cgcgcaaggc gaagaaggcc
     2041 acgccctcct ggaggggcac gatctcgccc tcgcgatcag ataaccggcc ttcgcccgcg
     2101 atccccagca cgaagccggc ctcgatcacg ccctgggcgc gcttcgtggc atcgacgaga
     2161 tccgacctcg acggcttgaa gggcaccgcc atgtgactcc aattgattac gtggttccgc
     2221 cagccgacgc tcatgtcctc ttccttggga ccgaacacat agatgcgatg ccggcgcggc
     2281 caccagccga ccagcaggaa cgggtcgacc cagccggggt ggttgaagtt caggacgtac
     2341 ggcggctccg gcagcggcgc caggtactcg tagcgaacct tcaggtacgt gccgaaaacc
     2401 agccgcgtcg cgtagcggat gaaccagcgc ttgagccggc ccggcgggtc aggcgccggc
     2461 agcgaactcg ggttcaagac gggctacggg gggcagcgcg acgagtggcg gcacaccgag
     2521 agtgtcgcgc tccagctcgt cgagaaaaac cggcacgagg tcaggatcga actgcgcgcc
     2581 gcgcagccgg cgcagctcgt cggcggcgcg ctcgtgcgtc tgcgatggct gatagggccg
     2641 cccgtggacc atcgcctcgt aggcgtcggc gatggcgacg atccgcgcac cgagcgggat
     2701 gctctcgccg cgcagcccat cggggtagcc ggtgccgttg aagcgctcgt ggtgatggcg
     2761 aatgatcggc gcgaagacgc gcgaggcccg caacgggcgg atgatcatct cgccgatctc
     2821 cgggtggcgg cgcatgacta gccactcatc ggccgacagc ggcccctcct tccgcagcag
     2881 gtgctcggac acgccgatct tgcccacgtc gtgcagcagc gcgccgtagg cgaccgcctc
     2941 gagctcctcg cctctcagcc ccacgttggc gccgactcgc gccgcgtgat gggccagcca
     3001 gcggcagtga tgc
//
LOCUS       DATBRX010000165         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2632129, whole genome shotgun sequence.
ACCESSION   DATBRX010000165 DATBRX010000000
VERSION     DATBRX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632129"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1112
                     /locus_tag="VIF08_06310"
     CDS             168..1112
                     /locus_tag="VIF08_06310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8135637.1"
                     /translation="MNTRPAIWGAALGSALALFVVAVALVMSGPGGAPTNGTPTPSGV
                     GHATPGASTGPGTPGPDATPTAQPTPLSTDKVEAPRSVGPQQLTGYGWPLKRPWITSR
                     FAPRDFGTFLVIDGVQYHDGLDLATHCGDEVYATHDGTVLVADRTFDQFLGYKGHPEE
                     IYNRTVRNGHKTALAIGVVIDDGNGYRSQYIHLKEATVVPGQVVRRGDQIGIEGATGA
                     ARGCHVHYSLIRMDGGWQAIIPRLWQYGYPEKIREHVNPLDVLPWGDEWAPLRLYNQI
                     HPPSPTPTPASPPPPTATPSPTPTGSPPTPSPSSSPRH"
     gene            1157..1279
                     /locus_tag="VIF08_06315"
     CDS             1157..1279
                     /locus_tag="VIF08_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135638.1"
                     /translation="MTTLRGALPIVRAFILAGLAIYLILFALPTVLGIAAAATP"
     gene            complement(1646..1894)
                     /locus_tag="VIF08_06320"
     CDS             complement(1646..1894)
                     /locus_tag="VIF08_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135639.1"
                     /translation="MTRLIGHHPRIEVDLDAAGQPERLRWEGTSMPVEVCNRWRVEEA
                     WWRRPVSRDYFKLAGTKLLALVYLDRVDGTWHLERVFD"
     gene            complement(1898..>2215)
                     /locus_tag="VIF08_06325"
     CDS             complement(1898..>2215)
                     /locus_tag="VIF08_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135640.1"
                     /translation="AAAPELLERLLFARLGNSMPAAAVTRLAVELRGEQVVAGQQLGL
                     FAPQLARAARLDWQLLGLALRFGPDRLLRARLLDPEAALVEARSELFPVSAGSRLTDQ
                     PAA"
BASE COUNT          361 a          810 c          716 g          328 t
ORIGIN      
        1 ccagatcgtc gacgctcgac gcgccgtcga ggaacgagaa gttggagtgg cagtgcagct
       61 cggcgaaggt gcccgacact cgaccatatt agaacagatg ttctagtggc agcattcgtg
      121 gtttcatacg gggacatgcc cagtctccag cgccccgtgg tgcgcccatg aatacgcgcc
      181 ccgcgatctg gggagcagcc ctgggcagtg ccctggcgct tttcgtcgtc gccgtcgccc
      241 tggtcatgtc cgggccaggc ggggccccca cgaacgggac gccaacgcca tccggcgtcg
      301 gccacgccac cccgggcgcg tccacgggtc ccggtacgcc cggtccggac gccacgccaa
      361 cagctcagcc aacgccgctc tcgaccgaca aggtggaggc gccgcgcagc gttggacccc
      421 aacagctcac gggctacggc tggccgctca aaagaccgtg gatcacgagc aggttcgcgc
      481 cgcgcgactt cggcacgttc ctggtgatcg acggggttca gtaccacgat ggactcgacc
      541 tggcgacgca ttgcggggac gaggtgtacg cgacacatga cgggaccgtg ctggtcgccg
      601 atcgcacctt cgaccagttc ctgggctaca agggccaccc cgaggagatc tacaaccgga
      661 ccgtccggaa cgggcataag accgcccttg cgatcggcgt tgtaatcgac gacggcaacg
      721 gctatcgcag ccagtacatc catctcaaag aggcaaccgt ggtccccggg caggttgtgc
      781 ggcgcggtga ccagatcgga atcgagggcg cgaccggcgc ggcgcgcggc tgccacgtcc
      841 actacagctt gatccggatg gacggtggct ggcaggcgat catcccgcgc ctctggcagt
      901 acggctatcc cgagaagatc cgcgaacacg tcaacccgct cgacgtgctg ccgtggggcg
      961 atgagtgggc gccgctgcgg ctgtacaacc agatccatcc accgtcaccg actccgacgc
     1021 cggcaagccc gccgccaccg accgccacgc ccagcccaac gcctaccggc tcacctccaa
     1081 caccctcgcc gagctcctcg ccgcgtcatt agccgccgcg cgtcacaggt cccgcgccgg
     1141 gtacgtcaca ggcacaatga cgacattgcg cggggccctg ccaatcgttc gcgcgttcat
     1201 cctggccggc ctggccatct acctgatcct gttcgccctg ccgacagtgc tcggcatcgc
     1261 cgcggcggcc actccctagc taactcggtg cggagcccgg cgccccagcc atccggctgg
     1321 ggtcgccggt actcaaagtc gtcggcgcaa tatccttggc gcgtggaagt gccgctccgt
     1381 caggtccttg ccgcgaccgt gctggtgctc gtgctggcca gcctcgtcgt cgcgatcgtc
     1441 ctcttgacca ttccgccggg cggcccgccg acgctgcact agtcggtcgc ctcgtcacac
     1501 ggcccggcgc gactgcgtct gatctgcaac tgcggtcgcc caaggactga cgtccggctc
     1561 ctcctctccg gacgccaacg cggctcgcgt ttcctcggcc ccggtctccc ccggggccga
     1621 gacattttct ggcgcagtcg gcggcttaat cgaagacgcg ttcgaggtgc cacgtgccat
     1681 cgacgcggtc caggtagacg agtgccaata gcttcgtacc ggcgagcttg aagtagtcgc
     1741 ggctgaccgg ccggcgccac cacgcctcct cgacgcgcca gcggttgcat acctcaacag
     1801 gcatgctggt gccctcccag cgcagccgct ctggctggcc ggcggcgtcg agatcgactt
     1861 cgatccgcgg gtggtggccg atcaggcgag tcaaggctca cgccgcaggc tggtcggtca
     1921 gacgcgaccc ggcgctgacc gggaacagct ccgagcgcgc ctcgaccagc gccgcctccg
     1981 gatcgagcag ccgggcgcgc aacaggcggt ccgggccaaa acgcagcgcc aggccgagca
     2041 gctgccagtc aagccgcgcg gcgcgggcca gctgcggcgc gaagaggccg agctgctggc
     2101 ccgcgacaac ctgctcgccg cgcagctcga cggccagacg cgtcaccgcc gcagctggca
     2161 tcgagttgcc caggcgcgcg aagagcaatc gctcgagcag ctcgggcgcg gccgc
//
LOCUS       DATBRX010000166         6657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1180106, whole genome shotgun sequence.
ACCESSION   DATBRX010000166 DATBRX010000000
VERSION     DATBRX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180106"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3459
                     /locus_tag="VIF08_06330"
     CDS             <1..3459
                     /locus_tag="VIF08_06330"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8135641.1"
                     /translation="RLWVHNFHSFGNRILSDHRADLGLTDSSDVLDGVGQRLLLRELR
                     PRFAHFLYHPMARDLNGAARFADVISRAKDELVQPDEYMAYANRKRAEFDAAHGEGEF
                     EKAVADLRERESSGTMWQINSVRRDLIKEGRDKALKSAYREARRAASGTGDLLWPSEM
                     TPDQLNLAKGLQPTFIRDAEALEVLRVSEEAEAYATYQQTLRERGLLDFGEQQALAIQ
                     LLIERPNILRRYQDQFRHVLVDEFQDANMAQILLLELVGRGPNKPDNVVVVGDDDQSI
                     YRFRGASYAAFSRFEERFELAPSWDPDRPAQKVVSQPLLKNRRSTGHILSAASRLIAR
                     NQKRLKSGPLTPIKDAGEPVRVVHATDEADEADLVVGWIKDTFASLPRPRRWSDIAVL
                     YRRHRHRELIVERLRKQDIPYVVVGGAGLFHVPDVRDVVAALRVAANPEDNASFVRLL
                     SAGPWRLDAAEILRVANAAAWDGRAVYQAASEILREGEINVSEPPAAALPKDAPMRAA
                     LGQTLWTDADFDEREPETVRQRRTREQRAVWRREQLDARLRVKLERLLGLLNELVPRG
                     QRDGPFAVLEDFMVRTNLLHDLIAVETPDAQRAVLALARLMRFVADWQVAHPRDSLGQ
                     FVAYLDLYEQVGGDLESDQVGRVEVEGVQLMTVYQAKGLEYEAVAVPRLVETQFPDTR
                     DEQRLLPIELLKQKPPDDFAIDEERRLLFVAMTRARSRLLLSALQPSGSKIQPSRFIA
                     EIERGGPALAPVSSDEYERPAPPDDIVIEQRAPTASSDVDDLGRGALETTPETTVQLL
                     KLMPVPLAHEQRFALRRRAIEIIGMLEGLAPEDAEGRRSLTHELVEVAEAAANAATEA
                     RRNGLDPLTLNVLSRHSPAGKTLLELAALPQTFSHSQLRMYDECPLQYAFNKVYRIPV
                     ATTPGYFEFGHVIHSAFEVYARTRRDAIAAGLPPPGYEALKQAFDAAWEPRNYVDIQA
                     AEHYAKRAEPALRRFYDREVKTIAQAVAFEQGFTLELKAGEGEAPVLLYGIIDRIDRH
                     ADGTIEITDYKTGRSKKQAEVDADEQLSAYALATAMGAVLDPATKEPLPAATRLTLYF
                     TENDLSLSTTRTPQQLEQFRESLIEKARRIRSGDFTATPDMWRCGRCDYRLICPSRFG
                     SERAIS"
     gene            complement(3470..4393)
                     /locus_tag="VIF08_06335"
     CDS             complement(3470..4393)
                     /locus_tag="VIF08_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135642.1"
                     /translation="MAMHRPQPLAVIALFALLAVACTGTGGPTGSPGPTGSPGQSATP
                     GASATPGASTTPAPSPSPVGNADVAVIRIEQTPGMLPPWESMRWYPYVALYGDGRLIV
                     QGPQMDIYPGPALPNLQVTHFSQHAVEQALAWAAEAGLHGEDRRLGTPIMDAGALVFT
                     IVSPQGTHHTSITGDDGNDPQITAAREFQDVMTSLATWLPEDVASESAPFQFDRLRVI
                     SFPADPQSLPDPAFATTVDWPLDADLASLGVSWSEPAQYRCFELSGDDLATALPVFAA
                     ANELTLYESADVTYQLYLHPLLPDDEACPGF"
     gene            4470..5417
                     /locus_tag="VIF08_06340"
     CDS             4470..5417
                     /locus_tag="VIF08_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135643.1"
                     /translation="MQVMTVTGPLPTDEMGVTDAHDHLYLRSPLLGGEEIDDPAIVEA
                     ETRDGARSGIATIVELTPIGLGRQPAALRALSEKTGVHIIGATGYHRDAHYPAGHWVL
                     DATDDELLARMAADLAQGMHPADWSDPGLLPDSARAGVIKAGASLDEVSANERRRLVC
                     AAAAAADAGVAVVVHCEAATYGEEIADLLLREGLPAGQIILAHMDRDPDAARHLDLLA
                     RGVFLVYDTIGRFQYHPDSIRIELIEAVCAAGRGDQLLLGLDLGKRSYLRVEGGHGLR
                     YLMDEFVPMLRERVGGAMTQRLLIDNAAAAFALRTPVAA"
     gene            5414..>6657
                     /locus_tag="VIF08_06345"
     CDS             5414..>6657
                     /locus_tag="VIF08_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8135644.1"
                     /translation="MSSRQVADRYQVVVVGAGSAGSSAAISAARTGARTLLIDRLGFM
                     GGTSTAVLDTFYAFYTPTPEPLRVVGGVGWEVVRRLTDAGLCFERPNTYGAGTGVTYD
                     QETLKLVWEGLAAESGVELLLHTWATGVRVEDGRVTGVRVWNKGGERWIGADVVVDAS
                     GDADVAALAGARYEDFGTTGRVQSLSTLFRLANVDVETATLTKKAELWALMREAAATG
                     AYELPRLEGSWHRTPFPGIVMIHMTRVPNVDATDPVALTDAELEGRRQVREYHRFLRD
                     RVPGFGQSVIVGTSPSIGVRESRRVIGDYQLTRDDVLGARRFDDEIALCGAPIEDHGP
                     GGDTDWQYVASGGHGAVYGIPYRSLLPRGLDALLVAGRCFSATHDAHASARSMATCMA
                     MGQAAGTAAALAASANSSPRAI"
BASE COUNT         1066 a         2276 c         2337 g          978 t
ORIGIN      
        1 cggctgtggg tccacaactt ccactcgttc ggcaatcgca tcctgagcga tcaccgcgct
       61 gatctcggcc tgactgacag cagcgacgtg ctcgacggcg tcggccagcg cctgctgctg
      121 cgcgagctgc ggccgcgctt cgcccacttc ctgtaccacc ccatggcgcg cgacctgaac
      181 ggtgcggcgc gctttgccga tgtcatcagc cgcgccaagg acgagctcgt ccagcccgac
      241 gagtacatgg cctacgccaa ccgcaagcgc gcggagttcg acgccgcgca tggcgagggc
      301 gagttcgaaa aggcggtcgc ggacctgcgc gagcgcgagt cgagcggcac gatgtggcag
      361 atcaacagcg tccggcgcga cctgatcaag gaaggccgcg acaaggcgtt gaaatcggcc
      421 taccgcgagg cacgccgcgc cgcgtcgggc accggcgacc tgctgtggcc cagcgagatg
      481 acgcccgacc agctcaacct ggccaagggc ctccagccga ccttcatccg cgacgcggag
      541 gcgctggagg tgctccgcgt gtcggaggag gcagaggcct acgcgacgta tcagcagacg
      601 ctgcgcgagc gcggcctgct cgacttcggt gagcagcagg cgctggcaat ccagctgctg
      661 atcgagcggc ccaacatcct gcgccgctac caggaccagt tccgccacgt gctcgtcgac
      721 gagttccagg atgcgaacat ggcccagatc ctgctgctcg agctggtggg ccgcgggcca
      781 aacaagcccg acaacgtcgt cgtggtcggc gacgacgacc agtccatcta ccgcttcagg
      841 ggcgcctcat acgcggcgtt cagccgcttc gaggagcgct ttgagctggc gcccagttgg
      901 gatcccgacc gaccggcaca gaaggtcgtc agccagccgt tgctgaagaa ccggcgctcg
      961 accggccaca tcctgtcggc cgcaagccgg ctcatcgcgc gcaaccagaa gcggctcaag
     1021 agcgggccgt tgacgcccat caaggatgcc ggtgagccgg tgcgcgtcgt ccatgcaacg
     1081 gatgaggccg acgaggccga cctggtggtc ggctggatca aggacacgtt cgccagcctg
     1141 ccgcgcccac ggcgctggag cgacatcgcg gtcctgtatc ggcgccatcg ccaccgcgag
     1201 ctgatcgtcg agcggctacg aaaacaggac atcccctatg tggtggtcgg tggagccggc
     1261 ctcttccacg tacccgacgt gcgcgacgtc gtcgcggcgc tgcgcgtggc cgcgaacccg
     1321 gaggacaacg cgtcattcgt gcgcctgctg tccgccggtc cttggcggct tgacgcggcg
     1381 gagatcctgc gcgtcgccaa tgccgcggcg tgggacggcc gtgccgtgta ccaggccgcg
     1441 tcggagatcc tgcgcgaagg cgagatcaac gtgagtgagc cgcccgccgc ggctctgccc
     1501 aaggacgcgc ctatgcgcgc ggcgctcggc cagacgctct ggacggacgc ggacttcgac
     1561 gagcgcgagc cggagacggt ccgccagcgc cgcacgcgcg agcagcgcgc cgtgtggcgg
     1621 cgtgagcagc tcgacgcgcg tctgcgggtc aagctcgaac ggctgctggg gctgctcaac
     1681 gagctcgtgc cgcgcggcca gcgcgacggg ccgttcgcgg tgctcgagga cttcatggtc
     1741 cgcaccaacc tgctgcacga cctgatcgcg gtggagacgc ccgacgcgca aagggccgtg
     1801 ctcgctctcg cccggctcat gcgcttcgtc gccgactggc aggtcgccca tccgcgcgac
     1861 agcctcggcc agttcgtggc ctatctcgac ctgtacgagc aggtcggcgg tgatctcgag
     1921 tcggaccagg tgggccgggt cgaggtcgag ggcgtccagc tcatgacggt ctaccaggcc
     1981 aagggcctgg agtacgaggc ggtggcggtg ccgcggctcg tcgagaccca gttccccgac
     2041 acgcgcgacg agcagcgact gctgcccatc gagctgctca agcagaagcc gccggacgac
     2101 ttcgcgatcg acgaggagcg gcgcctgttg ttcgtggcca tgacccgtgc ccgcagccgc
     2161 ttgctgctga gcgccctgca gccgtccggc tccaagatcc agcccagccg cttcatcgct
     2221 gagatcgagc gcggcgggcc tgcgctcgcg ccggtctcgt cggacgaata cgagcgcccg
     2281 gcgccgcccg atgacatcgt tatcgagcag cgcgccccca cggcttcgtc tgatgtcgat
     2341 gacctgggtc gcggcgcgct cgagaccacg cctgagacga cggtccagct gctcaagctc
     2401 atgcccgtgc cgctcgccca cgagcagcgt ttcgccctgc gccggcgcgc gatcgagatc
     2461 atcggcatgc tcgaaggtct ggcgccagaa gacgcggagg gccgccgctc gctgacccac
     2521 gagctggtcg aggtcgcgga ggcggcggcc aacgcggcca cggaggcgcg ccgcaacggc
     2581 ctcgacccgc tgacactcaa cgtcctgtcg cgccactccc cggcaggcaa gacgctcctc
     2641 gagctggcgg cgctgccgca gactttctcg cacagccagc tgcgcatgta cgacgagtgc
     2701 ccgctccagt acgcgttcaa caaggtctat cgcatcccgg tggccacgac gcccggctac
     2761 ttcgagttcg gccacgtcat ccacagcgcg ttcgaggtct atgcccgcac acggcgcgat
     2821 gcgatcgccg cgggattgcc gccgcccggt tacgaggcgc tcaagcaggc tttcgacgcg
     2881 gcctgggagc cgcgcaatta cgtcgatatt caggcggctg agcactacgc caagcgcgcc
     2941 gagccggccc tgcgccggtt ctacgaccgc gaggtcaaga ccatcgcgca ggccgtcgcc
     3001 ttcgagcagg gcttcacgct cgaactcaag gccggcgagg gtgaggcgcc ggtcctgctg
     3061 tacggcatca tcgaccgcat cgaccgccac gccgacggca cgatcgagat caccgactac
     3121 aagaccggcc gctcgaagaa acaggccgag gttgacgcgg acgagcagct gtcggcgtat
     3181 gccctggcga ccgcgatggg cgccgtcctc gatcccgcga ccaaggagcc gctgccagcc
     3241 gcgaccaggc tgacgctcta cttcactgag aacgatctga gcctctcgac cacgcgcaca
     3301 ccgcagcagc tcgagcagtt ccgcgagtcg ctgatcgaga aggcgcgccg catccgcagc
     3361 ggcgacttca cagcgacccc ggacatgtgg cgctgcgggc ggtgcgacta ccggctcatc
     3421 tgcccgagcc gcttcggatc ggagcgcgcc atctcgtagg cggcgggggc tagaagcccg
     3481 ggcaagcctc gtcgtcgggg agcagcgggt gcagatagag ctggtaggtc acgtcggccg
     3541 actcgtagag cgtcagctcg ttggccgcgg cgaacaccgg cagggccgtg gccaggtcgt
     3601 cgccgctgag ctcgaagcag cggtactgag cgggctcgga ccacgacacg ccgagcgatg
     3661 ccaggtcggc gtcgagcggc cagtcgaccg ttgtagcgaa tgccggatcg ggcaggctct
     3721 gcggatcggc cgggaacgag atcacacgca gccgatcgaa ttggaatggc gccgactcgc
     3781 tcgcgacatc ctcgggcagc cacgttgcga gactggtcat cacgtcctgg aactcgcgcg
     3841 cggccgtgat ctgcgggtcg ttgccatcgt cgcccgtgat cgaggtgtgg tgcgtgccct
     3901 gaggggacac gatggtgaag accagggcgc cggcatccat gatgggcgtt cccagcctgc
     3961 ggtcctcgcc atgcagccca gcctcggcgg cccaggcgag cgcctgctcg accgcgtgct
     4021 gtgaaaagtg cgtcacctgg aggttgggca gcgccggacc gggatagatg tccatctgcg
     4081 ggccctgcac gatgagccga ccgtcaccat agagcgccac gtagggatac cagcgcatcg
     4141 actcccaggg cggcagcatg cccggcgtct gctcgatgcg aatgaccgcg acgtcggcat
     4201 tgccgaccgg cgacggtgat ggcgcgggag tggtgctggc gccgggggtg gccgatgcac
     4261 ccggggtggc cgattgaccg ggtgagccgg tcggaccggg tgagccggtc ggaccgccgg
     4321 tgcccgtgca ggcgacggcc aggagcgcga aaagcgcaat aacagcgagg ggctggggcc
     4381 gatgcatggc catcagacgc tcatcgtgcc cagcagttcc aagcggccac ggacttgttg
     4441 cggggctcgc ggtgtcatga tcagcatgga tgcaggtcat gaccgtcacc gggccgctcc
     4501 ccacagatga gatgggcgtc accgatgccc acgatcacct ctatctgcgc tcgccactgc
     4561 tcggtggcga agagatcgac gacccagcga tcgtcgaggc agagacacgc gacggcgccc
     4621 gatcgggcat tgccacgatc gtcgagctga cgcccatcgg tctcggtcgc cagccggccg
     4681 cgcttcgtgc gctttccgag aaaacaggcg tccacatcat cggcgcgacc ggctatcacc
     4741 gtgacgcgca ctaccccgcg gggcactggg tgctggatgc caccgatgac gagctcctgg
     4801 cgcgcatggc cgccgatctg gcacagggca tgcacccggc cgattggtcg gatcccggcc
     4861 tgctgcctga ctcggccagg gcaggggtga tcaaggccgg cgcgtcgctg gatgaggtct
     4921 ccgccaatga gcgccgacgc cttgtttgtg ccgccgccgc ggccgccgac gctggcgtgg
     4981 ccgtcgtcgt gcattgcgag gcagcgacct acggcgagga gatagccgac ctgctgctgc
     5041 gcgagggttt gcccgcgggg cagatcatcc tcgcgcacat ggaccgcgat ccggatgccg
     5101 cgcgccactt ggacttgctc gcgcgcggcg tgttcctggt ctacgacacg atcgggcgtt
     5161 tccaatacca ccccgattcg atacgcatcg aattgatcga agcggtgtgc gcggcgggcc
     5221 gcggcgacca gcttctgctc ggcctcgatc tgggaaagcg cagctacctg cgggtcgagg
     5281 gcgggcacgg cctgcgctac ctgatggacg agttcgtgcc gatgctgcgc gagcgcgtgg
     5341 gcggggcgat gacgcagcgg ctgctcatcg acaacgcggc cgccgcgttc gcgcttcgga
     5401 cgccggtcgc ggcatgagca gccgtcaggt agcggatcgc taccaggtcg tggtggtcgg
     5461 ggcgggctcg gccggttcgt cggcagcgat cagcgccgcc aggacaggcg cgcgaacgtt
     5521 gctcatcgat cggctgggct tcatgggcgg cacctcgacc gcggtcctcg acacgttcta
     5581 tgcgttctac acgcccacgc cggaaccgct gcgtgtcgtc ggcggcgtgg gctgggaggt
     5641 cgttcggcgg ctgaccgacg ccggtctctg cttcgagcga cccaacacgt atggcgcggg
     5701 caccggcgtg acctacgacc aggagacgct caagctcgtg tgggaggggc tcgccgcgga
     5761 gagcggtgtc gagctgctgc tgcacacctg ggcgaccggc gtgcgtgtcg aggacggacg
     5821 cgttaccggg gtgcgcgtct ggaacaaggg cggcgaacgc tggatcggcg ctgacgtggt
     5881 ggtcgacgcg tcgggtgacg cggacgtggc cgcgctcgcc ggcgcgcggt acgaagactt
     5941 cggcacgacc ggccgcgtcc agagtctgtc gacgctgttc cgcctcgcga acgtggacgt
     6001 ggaaacggcc acactaacga agaaggccga gctgtgggca ttgatgcgcg aggcagccgc
     6061 aacgggtgca tacgagctac cgcggctcga gggctcatgg caccgcacgc cgtttccggg
     6121 catcgtgatg atccacatga cgcgcgtccc caacgtcgac gcgaccgacc cggtcgcgct
     6181 gaccgacgcc gagctggaag gccggcgcca ggtgcgcgag taccaccgct tcctgcgcga
     6241 ccgcgtcccg ggattcggcc agtcagtgat cgtcggcacc agcccgtcga tcggcgtgcg
     6301 cgagtcgcgg cgggtgattg gcgactacca gctgacgcgc gacgacgtgc tgggcgcgcg
     6361 gcggttcgat gacgagatcg cgttgtgcgg cgcgcccatc gaggatcacg gcccaggcgg
     6421 cgacacagac tggcagtacg tggcgagcgg tggccatggc gcggtctacg gcatcccata
     6481 ccgctcgctg ctgccccgag gcctggacgc gctgctcgtc gccgggcgct gcttcagcgc
     6541 aacgcacgac gcgcacgcct cggcccgcag catggccacg tgcatggcca tgggccaggc
     6601 ggcaggaacg gccgcggcac tcgcggccag cgccaattcc tcaccgcgcg cgattga
//
LOCUS       DATBRX010000167        14877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3190992, whole genome shotgun sequence.
ACCESSION   DATBRX010000167 DATBRX010000000
VERSION     DATBRX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14877
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3190992"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VIF08_06350"
     CDS             complement(<1..818)
                     /locus_tag="VIF08_06350"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8135645.1"
                     /translation="MVNQMDRASQLEASAAVPSRMSKGGVAWIVFVLLLLGVHVLVPQ
                     RSGFLALTEVLEPYVVVTALIAAPLALIDRNRAGSVVVALLVVVVLGRYVPGCTSLPA
                     SGVPRLTVATWNIFAGPNDHKRAVAGVLAANTADLIALVELRPGAANALAAEPELLEH
                     LPYRFLAPQPGITGAGLMSRFPIQGDWLSTDPPFLRAEVALPDQTLVVYVVHPLSAGI
                     SSLSRIPRSLETGARDAALRTIRAFIDTDIAAGRSIIVAGDLNTTEREPAYVDFS"
     gene            881..1288
                     /locus_tag="VIF08_06355"
     CDS             881..1288
                     /locus_tag="VIF08_06355"
                     /inference="COORDINATES: protein motif:HMM:NF045421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135646.1"
                     /translation="MAKGSGTKEDPWQLKTPPGTSEYQMWIDESSDPPAIVCQVGGTQ
                     LKYQKRAIDDLHAMLKKHGDWMDLGGADEQKPAAEGTVEAWGRSEKNPVGGWYGMKKG
                     LRGRFGVYMPPLMEALGLVELTHDAKNNRMRAK"
     gene            complement(1334..3136)
                     /locus_tag="VIF08_06360"
     CDS             complement(1334..3136)
                     /locus_tag="VIF08_06360"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEY8135647.1"
                     /translation="MSFTFGVTLLAIGLFGSAFAVGYARVNEGRVLPGVDVGGVALSG
                     LDRPSAAARLRQSLPDLSQGNLVIEIGDTSESVPFSAIGRGYDMDFMLDQALGVGRAP
                     NFVEQLQEQLRTLISGTSVNPVAAWDNDALAQQVSAIARAAQWDPIDASLSRVDGRYV
                     VNPSSPGQSVDVEGALSSALAAVDNVSPADAHVNVATTVVPPRIETATAQAAADLAEQ
                     VMGTDLRVTGPAQSMTITSAELRGWVHLDLNAEGTNWQLVIERAPIAQAVVSYGLAND
                     IAPQNASFKFQDGGIAVVPSSDGLAANVETTTDSIMAVLEGRAAGTLGGVATLTLDSV
                     PPTFTTAEATALAPQITLLGEWTTNYVPSPLNGEGVNIQIPTKIIDGYVVEPGGLFDY
                     LSVIGPITSPPYTSGAAIVRGHTVLEGVLGGGMCSSSTTIFNAALRAGLDMRNRGNHF
                     YYISRYPLGLDATVWITKSRRLTMSFVNDTAYPILIRGINQTGKVTFQIFGVDDGRTV
                     EFTEPIVTNPVAAVDRMMYVDALPAGIKKRVEFNVDGMDVSVTRIVRDAAGNVIHEDT
                     YVSDYGTIDGLTEVGRYPGDPPDGTIIPASAYPH"
     gene            complement(3327..4418)
                     /gene="jag"
                     /locus_tag="VIF08_06365"
     CDS             complement(3327..4418)
                     /gene="jag"
                     /locus_tag="VIF08_06365"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEY8135648.1"
                     /translation="MSEYQEFTGKTVEEALREARDAFGAAGLEELDFEILTPGSRGVL
                     GMGAEPARIIAAPRSALGGAAPRREAAAQTPLPPPPPRESFGDRADGGDRGDRPPRRE
                     GGFGRDRGGRDRGPSREGGFRSDRPAAPAGEAPSGESRVGYRDRPPRREGGFGRDRAD
                     RGPRREGGFAGRERSSGTLSESEQEEVRAGRASLSVEREEIQAAEASPEALEAGREIL
                     AQLLGHMGLSVSVSVETGDTSKLDVRGDEQSKEALGALIGRKGERLSALQHLVNLMLS
                     RKLGAWTRVLVDVEDYRGRRERQLREIATRAAEKVKESGKMLQLEPMPALERRWIHLA
                     LRGDADVATQSIGEEPHRRVVILRRQTGG"
     gene            complement(4415..5770)
                     /gene="yidC"
                     /locus_tag="VIF08_06370"
     CDS             complement(4415..5770)
                     /gene="yidC"
                     /locus_tag="VIF08_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY8135649.1"
                     /translation="MTLSADMRAHSLRRALLLVMALLALGVALFGALPASADTPSPSI
                     APTAAPTTLPSPTTEPTPTSGSPLPGGANPAVSQPPCPLPTYTPVPATPTPAATLAPG
                     ATAAPVPTPVHPALCPAVMNQPTDILAIAFNPIFQTMFLLLTIFFALTRDIGIAIILL
                     TLLIKTILIPLFRAQIVSQRRMQILQPEIRAIGIKYKGNRTKINEEQMRLYRERGVNP
                     ASGCLPSMLQLFLLLPIYQVISQGLAAPDISSALQFMGVPVLQDFACQALGTLQPCIN
                     PNIWWLGNLDAHVPEVLIHLPNPIISDPRFGLSGLALVAAVLQLIQSRMMTQRSSDPQ
                     TQSQQRIFLILPLFSLFYGWFLPAGLFIYWIVFTAYSIVQQYLIVGWGSLFPLFGWTP
                     GFARDHQPRFPVTSAPIKRADSAADGRSTTDSRSPSDRAAGTIRPDRQRGRTSRRGRR
                     R"
     gene            complement(5754..5969)
                     /gene="yidD"
                     /locus_tag="VIF08_06375"
     CDS             complement(5754..5969)
                     /gene="yidD"
                     /locus_tag="VIF08_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY8135650.1"
                     /translation="MNPMKWFGVGAIRLYRAVFFWLPSSCRFEPSCSRYTEQAIVKYG
                     LLKGSWMGAKRIARCQPLHPGGYDPVR"
     gene            complement(5966..6328)
                     /gene="rnpA"
                     /locus_tag="VIF08_06380"
     CDS             complement(5966..6328)
                     /gene="rnpA"
                     /locus_tag="VIF08_06380"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY8135651.1"
                     /translation="MTAVAKSSVGLDMLRSGVAFRAIQTDSRSRSHPLLLLRYRGNGL
                     EQTRFGISTSRRLGSAVVRNRQRRRLRSILRELAPGLAPGWDVLLVLRPAAADASQAQ
                     LEPAVAGLMRAAGLVAAQ"
     gene            complement(6329..6466)
                     /gene="rpmH"
                     /locus_tag="VIF08_06385"
     CDS             complement(6329..6466)
                     /gene="rpmH"
                     /locus_tag="VIF08_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY8135652.1"
                     /translation="MGKPTFQPKKRHRAKEHGFRKRMRTPGGRRVLAARRAKGRKQLT
                     A"
     gene            6621..6977
                     /locus_tag="VIF08_06390"
     CDS             6621..6977
                     /locus_tag="VIF08_06390"
                     /inference="COORDINATES: protein motif:HMM:NF007494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEY8135653.1"
                     /translation="MTDFQPPGSATMDDFQRIDMRVGRIVAAEEFPRARVPAYRLTVD
                     FGPHGTRRSSARLAGTYPDPAALVGRLVIAVVNFAPRNIAGFESQVLVLGALPDDGLI
                     PLLSVDSGAQPGDSIG"
     gene            7220..8599
                     /gene="dnaA"
                     /locus_tag="VIF08_06395"
     CDS             7220..8599
                     /gene="dnaA"
                     /locus_tag="VIF08_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY8135654.1"
                     /translation="MDAKQVWRAALGELQVSLSPANFETWLKETALVAVDDNRFRVAV
                     PNGFAKDWLETRYRSLISQTLARIVGYSVQVDFEVREGVAAGSAEEAEGAAQANAAPN
                     PQVRLEPGRVGGPEGASASLNPRYTFRNFIVGSANRLAHAASLSVAERPGHAYNPLFL
                     YGGVGLGKTHLMHAIGNQVLARFPRKRVVYASSEKFTNDFITSIQQGKIDEFRGRYRR
                     IDLLLIDDIQFIADKERTQEEFFHTFNAIHEDGKQIVLSSDRPPKLITTLEERLRSRF
                     EWGLIADLTAPDLETRIAILRAKAEEQQVHIGSDVLEFIARKVISNIRELEGALNRIV
                     AYANMGGMPLTNDLAQAVLSNVLYNPKRRLVTPERIVHAVSEYYSVELDALKGQKRDR
                     AIVMPRQVAMFLIRAETDVSLLRIGQELGGRDHSTVLHACEKIDRETQENEELRRDLA
                     AVRELIYSE"
     gene            8939..10057
                     /gene="dnaN"
                     /locus_tag="VIF08_06400"
     CDS             8939..10057
                     /gene="dnaN"
                     /locus_tag="VIF08_06400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY8135655.1"
                     /translation="MKLSVMQENLARGLGIVSRAVSSRSTLPVLSNVLLRTEDAGLKL
                     TATNLEIGITCWVAGKIDAEGAMTVPARLFNDLVAGLPAGERIDLEADDATTLRIKAG
                     RYQTQLRGIDAEEFPVIPSPGERPTTRVNQKELRHALSEVVFAAASDEARPILTGVLT
                     RLSGDKLTLAAADNYRIAVRSLAILDPVEDTSLVVPARSYAELMRVMTDSDDPVEIML
                     ASSKSQVLFHVNDIDIVSRLIDGQFPNYQQVLPSSHSTRAVVDRDELLKAVRLSALIA
                     SSAANVVRLRLGDDGSGTINVAAAADVGEAQGDVEAAIEGEGVQISFNARYLQEALQS
                     LTQDQLALEFAGPLSPGVLKPTDDEEYVHVIMPVRTPS"
     gene            10092..11213
                     /locus_tag="VIF08_06405"
     CDS             10092..11213
                     /locus_tag="VIF08_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEY8135656.1"
                     /translation="MRVTQLSLAGFRSYAALSADFPPGPQVVVGDNATGKTNLLESLV
                     VLGTGHSHRAGADAELVEWGADFARLEATVSGAGGSATQLEVVIAMAAGGGGRKRVQV
                     NGVARRPTALSAALPVVLFAPEDMLLISGGPTLRRTALDTLVAQATPGASATLSTYAR
                     AITQRNNLLRAIREGTAAPDELRFWDEVVIDDGARIVDWRRDALARLAEPLAAAHAEI
                     APAEERLELRYATNAERLAAESTSDALRRRLADTAEKEQWNGATLIGPHRDDIVFTSS
                     ARDLADFASRGQQRTAILAFKLAQLDLLRSATGEAPLLLLDDVFSELDPSRRAHLVRR
                     IGELPQAFVTTTTTDDLDPHLVAAATVWNVTPGRLERAA"
     gene            11236..11592
                     /locus_tag="VIF08_06410"
     CDS             11236..11592
                     /locus_tag="VIF08_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEY8135657.1"
                     /translation="MSPRRPMKRIGDMLPDVAAGLGIDAELRLARQMAAWERLVSERV
                     PAATGRSRLLSIQPPALVVSASDPRVGLELRMRQAELLTAFAQAPEGVRLLELRVVVR
                     PPGATNPPPGRSGGPR"
     gene            11605..13884
                     /locus_tag="VIF08_06415"
     CDS             11605..13884
                     /locus_tag="VIF08_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135658.1"
                     /translation="MASRLQIKYGLVAEEDRLSSSSDALIVTEPTTGSKARTKGSLYL
                     ITTSRQTGGRTREACRLVADSIRREYYYDESAGIAIVLEKAIRASNRRLRHSREGNGL
                     PIGAIGIALAVVRGNEIYVATCGEADGYLVRSARLLMPEHDSGSGLPAADSLKVDVWR
                     GEFSVGDSLLLCSRNLVEAVGTEELKNAVVTLHPQSAAEHLHHLFVAAGGTGSDAVLA
                     IEASEVSLAKVEHRLVPVSPSEPLAGTPIASPIPLADKMAGAASAVQERAVAARGALR
                     QGVGRIVDHVLDLMPQRRTSLRRITAATTRRETQRRIALAVLGFIAVVAVVGVGLWYA
                     PQRGDRPIDQVNQGEAALADAVEAANLVFGPDDLLHSDPDEALVLLQGAWSDLAVARE
                     AQVDPQTIAQLDTQLRFGLETLYATATVTATQLYAAPEGARVSALAQGPDSAAYAIVG
                     DTVVRIDPETGAMTTVITAGDGLGGGIGVPRLLAHGGPDLLILDEDSRLWRWRPSGAL
                     GEVRLSGDQKWDETVVDFQAFIINLEQGLYRIYVPYPRTSQILRYDPTADGGGFSAPA
                     PYFISENVDVTRFRQLYVDGDIYAVTSENLEQYFSGRQTAFSLDPPPDDGDLRAGHDY
                     ALLSATGVRGDGDLYVWDKLWSRVLVYDKAAGAYSTQYLAADGTPPLADLTGMYVVDR
                     GHSEPPLLIFTTSSGLFRVELGAGAVPQPTASPAASASPPPTLTPSPYPTAPGETPSP
                     EPTRPRRTPRPVESPTAAP"
     gene            13912..14445
                     /locus_tag="VIF08_06420"
     CDS             13912..14445
                     /locus_tag="VIF08_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEY8135659.1"
                     /translation="MPGGANGHNGGLNEMIVESVRVHMLSSQHVVILRDTGHDRYLPI
                     WIGPWEANAIAMRLQGVTPERPMTHDLFSQALTELGVTLKRIVVSDLAEDTFRARLLL
                     QVDGRELDLDARPSDAIALAVRSGVPIFATDAVLDRAGVVPEGAEEEKLSVFREFVNS
                     LNVDLPDDPESGSRKLD"
     gene            complement(14455..>14877)
                     /locus_tag="VIF08_06425"
     CDS             complement(14455..>14877)
                     /locus_tag="VIF08_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135660.1"
                     /translation="AADNPDVVVGYRIKRADPFVRLAYARAYRLALRIFYGLKVRDPD
                     CACKLFRRAALDGISVESQGGFLSGELLVKLLQRGRTIAEVGVPHYPRIAGSQTGANP
                     KVVLRAVRDFWRLRLRLWFRRPGALDRGEPLLAEGPTT"
BASE COUNT         2421 a         5017 c         5149 g         2290 t
ORIGIN      
        1 gaaaagtcga cgtaggcggg ctctcgctct gtggtgttca ggtcgccggc cacgatgatc
       61 gaccggccgg cggcaatgtc ggtgtcgatg aaggcgcgaa tggtgcgcag cgcagcgtcg
      121 cgtgcgcctg tttccagcga ccttggaatg cgactgagcg atgagatgcc ggccgacagc
      181 ggatggacga cgtacacgac cagcgtttgg tcgggcaggg ctacctcggc acggagaaac
      241 ggcggatccg tcgataacca atcgccttgg atcgggaacc tgctcatcaa tcctgccccc
      301 gtaatgcccg gttgcggcgc gaggaatctg tagggcaggt gctcgagcag ctcgggctcc
      361 gcagccaggg cattggccgc acctggacgg agctcgacca gcgcgatcag atcggccgta
      421 tttgccgcca gcacgccggc cacggcgcgc ttgtggtcat ttggtccggc gaagatgttc
      481 caagtcgcca cggtcagtcg ggggacgccg ctcgcgggca gggacgtgca ccccggcacg
      541 taccggccca agacaacaac gaccaggagc gcgaccacca ccgaccctgc cctgtttcga
      601 tcgatcaggg cgaggggcgc tgcaatcaga gcggtcacga ccacgtaggg ttccagcacc
      661 tcggtgagcg ccagaaaccc cgatcgctga ggcaccagga cgtgtacgcc cagcaggagc
      721 agcacaaaga cgatccaggc gacgccgccc ttgctcatac gcgatggcac ggcagcgctc
      781 gcttcaagct gtgaggctcg gtccatctgg ttgaccattc atgagaatgc gacactcgag
      841 gaggattgtc gtgattcatg cgactggagg tgacaagcaa ttggccaagg gaagcggaac
      901 caaggaagat ccgtggcagc tcaagacgcc gcccggcacg tccgagtacc agatgtggat
      961 cgacgagagc tccgatccgc cggccatcgt ctgccaggtg ggcggcactc agctcaagta
     1021 ccagaagcgc gcgatcgacg atctgcacgc gatgctcaag aagcacggcg actggatgga
     1081 tctgggtgga gccgacgagc agaaaccggc ggctgagggg acagttgagg cctggggtcg
     1141 gtcagagaag aacccggtgg gcggctggta cggcatgaag aagggcctgc gcggccgatt
     1201 cggcgtctac atgccgccgc tgatggaagc gctcggcttg gtcgagctga cccacgacgc
     1261 caagaacaac aggatgcgcg ccaagtagac gagccggcgc tccaggtagg cgaggacacg
     1321 cgcctggtgg gagctagtgc gggtatgcgc tcgcggggat gatggtgccg tcgggcgggt
     1381 cacccgggta gcgcccgacc tcggtcaggc cgtcgatcgt cccgtagtcg gaaacgtacg
     1441 tgtcctcgtg gatcacgttg ccggccgcgt cgcggacgat gcgcgtgact gagacgtcca
     1501 tgccgtccac gttgaactcg acgcgcttct tgataccggc tggcagtgcg tcgacgtaca
     1561 tcatccggtc aacggcagcg accgggttgg tgacgatggg ctcggtgaac tcgactgtcc
     1621 gcccatcgtc cacgccgaag atctggaacg tcaccttgcc ggtctggttg atgccgcgga
     1681 tcaggatggg ataggccgtg tcgttgacga aggacatcgt caggcggcgc gacttggtga
     1741 tccatacggt ggcgtcgagg cccagcgggt agcggctgat gtagtagaag tggttgccgc
     1801 ggttgcgcat atccaggccc gcgcgaagcg cggcgttgaa gatggtcgtc gacgacgagc
     1861 acatgccgcc gcccagtaca ccctccagga ccgtatggcc gcgcacgatc gccgcgcccg
     1921 aggtgtaggg cgggctggtg atggggccga tcacggacag gtagtcgaac agccccccgg
     1981 gctcgacgac gtagccgtcg atgatcttcg tggggatctg gatgttgacg ccttcgccat
     2041 tgagcgggct gggcacgtag ttcgtcgtcc actcgccgag caaggtgatc tgcggcgcga
     2101 gggcggtcgc ctcagcggtc gtgaatgtcg gcgggacgct gtcgagcgtc agggtcgcga
     2161 cgccaccgag cgtgccggcg gcgcgcccct cgagcacggc catgatgctg tccgtggtgg
     2221 tctcgacgtt cgcggccaga ccgtcggagc tgggcacgac cgcgatgccg ccgtcctgaa
     2281 acttgaagga cgcgttctgc ggcgcgatgt cgttggccag cccgtagctc acgactgcct
     2341 gggcgatcgg ggcgcgctcg atcacgagct gccagttcgt gccctcggcg ttgagatcca
     2401 ggtgaaccca gccgcgcagc tcggccgacg tgatggtcat cgactgcgcg ggcccggtga
     2461 cacgcaggtc cgtgcccatg acctgttctg cgagatcggc cgcggcctgc gccgtggccg
     2521 tctcgatgcg cggcggcacg acggtggtgg cgacgttgac gtgcgcgtct gccggtgaga
     2581 cgttgtcgac ggccgccagg gcgctggaca gggccccctc gacatcgacg ctctggccgg
     2641 ggctcgaggg attgaccaca taacgaccat ctacccggct cagggaggcg tcgatcggat
     2701 cccattgcgc ggcgcgggcg atggcgctga cctgctgtgc cagcgcgtcg ttgtcccacg
     2761 ccgcgaccgg gttgaccgac gtgccgctga tcagcgtgcg cagctgctcc tggagctgct
     2821 cgacgaagtt cggcgcgcgg cccacgccga gcgcctgatc gagcatgaag tccatgtcat
     2881 agccacgacc gatcgcggag aagggcaccg actccgaggt gtcgccaatc tcgattacaa
     2941 ggttgccctg cgacaggtca ggcaatgact ggcgcaaacg cgccgcggcg gatggccggt
     3001 cgagacccga cagcgcgaca ccgcccacgt cgacgccggg caggacgcgg ccctcattga
     3061 cgcgcgcata gccgaccgcg aaggccgagc cgaagagacc gatcgccagc agcgtcacgc
     3121 caaacgtgaa gctcatgcgc cgcatcgttt ggctgatcag cggggtcggc gagacgccca
     3181 ccgggagaga ttgagtccgc gccattgcgc gggatcgtac caacattcgg catgtgccga
     3241 agggggcccg atggtctcgt tcgtatcagc cgggcggcta aacgctctct tggacgctgc
     3301 tccgatgagc ggttacgcgc ccgaggtcag ccgccggtct ggcggcgcag gatcaccacg
     3361 cgacgatgcg gctcctcgcc gatggactgc gtcgccacgt cggcgtcgcc gcgcagcgcg
     3421 agatggatcc agcgccgctc cagtgcgggc atcggctcca gctgcagcat cttgccgctt
     3481 tccttgacct tctccgcggc gcgcgtcgcg atctcgcgca gctggcgctc acgccggccg
     3541 cggtaatctt cgacgtcaac aaggacgcgc gtccacgcac cgagcttgcg cgagagcatc
     3601 aggttgacca ggtgctgcaa cgccgacagg cgctcgccct tgcggccgat caacgcgccc
     3661 agcgcttcct tgctctgctc gtcgccgcgc acatcgagct tggaggtgtc tccggtctcg
     3721 acgctcacgc tgactgacag gcccatgtgg cccagcagct gggccaggat ctcgcgcccg
     3781 gcctcgagcg cctcggggct cgcctcggcg gcctggatct cttcccgctc gaccgacagg
     3841 ctggcccggc cggcacggac ttcttcctgc tcgctctccg acagtgtgcc gctgctgcgc
     3901 tcccggcccg cgaagccgcc ctcgcggcgc gggccgcggt ctgcgcggtc gcggccgaag
     3961 ccaccctcgc ggcgcggcgg acgatcgcgg tagccgacgc gcgactcgcc cgatggcgct
     4021 tcgcccgctg gtgccgctgg gcggtcactc ctgaagccac cctcacgact tgggccccgg
     4081 tcgcgtccac cgcggtcgcg gccgaatcca ccttcgcggc gcggcggtcg atcgccacgg
     4141 tcaccgccat ctgccctgtc gccgaacgac tcgcgcggcg ggggcggggg cagcggcgtc
     4201 tgcgccgcgg cctcacgccg gggagcggcg ccgcccagcg ccgagcgcgg tgcggcgatg
     4261 atccgggcgg gctcagcgcc catgcccagc acgccccggc tgcccggcgt caggatctcg
     4321 aaatcgagct cctcgaggcc ggccgcgccg aacgcgtcac gcgcctcgcg cagtgcctcc
     4381 tcgactgtct tgccggtgaa ctcctggtat tcgctcatcg ccgtctccct cggcggctgg
     4441 tccgcccgcg ctggcggtcc ggccggattg tgcccgcggc cctgtcagat gggctgcggc
     4501 tgtctgttgt ggagcggcca tcagccgcag aatcggcgcg cttgatcggc gcactcgtga
     4561 ccgggaatcg cggctggtgg tcgcgcgcga aacctggtgt ccagccgaag agggggaata
     4621 gcgaccccca gccgacgatc aggtattgct gcacgatcga gtacgccgtg aacacgatcc
     4681 agtaaatgaa taggccggcc ggtaggaacc aaccgtagaa gagtgagaat aggggcagga
     4741 tcaggaagat gcgttgctgt gactgtgtct gcgggtcgct cgagcgctgg gtcatcatgc
     4801 gcgactggat gagctgcagc accgccgcca cgagcgccag cccggacagg ccgaagcgcg
     4861 ggtcgctgat gatcgggtta ggcaggtgga tcaggacctc gggcacgtgt gcgtcgaggt
     4921 tgcccaacca ccagatattc gggttgatgc acggctgaag ggtgcccagc gcctggcacg
     4981 cgaagtcctg cagcaccggg acgcccatga actggagggc cgagctgatg tccggcgcgg
     5041 ccagtccctg gctgatcacc tggtagatgg gcaggagcag gaagagctgc agcatcgacg
     5101 gcaggcagcc cgacgcgggg ttgacgccgc gctcgcgata gagccgcatc tgctcttcgt
     5161 tgatcttggt gcgattgccc ttgtacttga tgccgatcgc gcggatctcc ggctggagga
     5221 tctgcatccg ccgctgcgac acgatctggg cgcggaagag cgggattagg atcgtcttga
     5281 tgagcagcgt cagcaggatg atcgcgatgc cgatgtcgcg cgtcagcgcg aagaagatcg
     5341 tcagcagcag gaacatcgtc tggaagatcg ggttgaacgc gatcgccagg atgtccgtgg
     5401 gctggttcat gacggccggg cacagggcag gatggaccgg cgtgggcact ggggctgcgg
     5461 tggcgcccgg ggcgagggtg gccgccggcg tgggtgtcgc cggcaccggc gtgtaagtcg
     5521 gcagcgggca gggcggctgc gagacggccg gattggcgcc gccgggcagg ggtgagccgg
     5581 aagtcggcgt gggctctgtc gtcggtgagg gcagcgtcgt tggcgcggca gtcggcgcga
     5641 tcgagggcga aggtgtatcg gcactcgccg gcagagcgcc gaacagcgcg acacccagcg
     5701 ccagcagcgc catcacgagc agcagggcgc ggcgcagcga atgggcgcgc atgtcagcgg
     5761 acagggtcat aaccccccgg gtgcaacggc tggcagcggg cgatgcgctt ggcgcccatc
     5821 cagctgccct tgagcagtcc gtacttgacg attgcctgtt cggtgtagcg cgagcacgag
     5881 ggctcgaaac gacacgagga cggcagccag aagaagaccg cgcggtacag ccgtatggcg
     5941 ccgacgccga accacttcat gggattcatt gggcggcgac cagtccggcg gcgcgcatca
     6001 ggccagcgac tgccggctcg agctgggcct ggctggcatc tgcggcagcg ggtcgcagca
     6061 cgagcagcac gtcccagccg ggagcgagtc ctggtgcgag ttcgcgcagg atcgagcgca
     6121 gccgtcgacg ctgccggttg cggaccacgg cagaaccgag gcgccggctg gtcgatatac
     6181 cgaatcgagt ctgttcaagt ccgttcccgc ggtagcgcag cagcagcaag gggtggcttc
     6241 ggctgcggct gtcggtctgg atggcccgga aggcgacccc tgagcgaagc atgtcgagtc
     6301 cgacactgga cttcgccacg gcggtcacct aggcggtcag ctgcttgcgc cccttagcgc
     6361 ggcgcgcagc gagtacgcgc cgaccgccgg gcgtcctcat gcgcttgcga aaaccatgct
     6421 ccttggcgcg atggcgcttc ttgggctgga atgtcggttt tcccatatct gctgtggagc
     6481 tccctcggcg cgttgtcagg acggccgcgc tgcggccgat ctggctcaag attgagttgg
     6541 cgtgcgagcg ccgcagcacg tctgctcggg tcggagtata gccgcagccg cgccgcagcc
     6601 gatgaaaggg cacctgtgag atgaccgact tccagccacc gggcagcgca acgatggacg
     6661 acttccagcg gatcgacatg cgcgtcggcc ggatagtcgc ggccgaagag ttcccacggg
     6721 cacgcgtgcc ggcgtatcgc ctgaccgtcg atttcgggcc gcacggcacg cgccgatcat
     6781 ctgcgcgact ggccggcacg tatcccgatc cagcggcgct cgtcggtcgg ctagtcatcg
     6841 ccgtggtgaa cttcgcgcca cgcaacatcg ccggcttcga aagccaggtg cttgtccttg
     6901 gagcactgcc cgacgacgga ctgataccac tcctgagtgt cgacagcggc gcgcaacccg
     6961 gcgattccat cggttgagcc cgatactccc atggagtatc aacgattacg gttgcggccc
     7021 gcgcgggggc ggtgctattg tcgccgcccc cgagaccgtg agggaccgga gggaggagcg
     7081 agaacgcggt cggtcggctg gccgggaacc cagtcggagc cgttcgcgag gacaactttt
     7141 ttcacgggct ccgcgccgat tgcgtgtgct ctatcgcggc tggccgtggt caatgactca
     7201 tcacgaggcg gggctattga tggacgcgaa acaggtctgg cgcgctgcgc tgggcgagtt
     7261 acaggtttcg ctgtcgcctg ccaacttcga gacctggctc aaggaaaccg ccctcgtcgc
     7321 agtcgatgac aaccgcttcc gcgtcgccgt tccgaatggt ttcgccaagg actggctgga
     7381 gacccgctat cgctcgctga tcagccagac gctcgcgcgc atcgtcggct acagcgtcca
     7441 ggtcgacttc gaggtgcgtg agggagtcgc cgcgggttcg gccgaagaag ccgagggagc
     7501 ggcacaggca aacgcggcgc ccaacccgca ggtgcggctc gagcccggcc gcgtcggcgg
     7561 accggaaggt gcgagcgcca gcctcaaccc gcgctacacg ttccgcaatt tcatcgttgg
     7621 ctcggcgaac cgcctcgcac atgccgcttc actctctgtc gccgagcggc ccggccacgc
     7681 ctacaacccg ctcttcctct atggcggcgt gggccttggc aagacacacc tgatgcacgc
     7741 gatcggcaac caggtgctgg cgcgcttccc gcgcaagcgc gtcgtatacg cgtcgagtga
     7801 gaagttcacc aacgacttca tcaccagcat ccagcagggc aagatcgacg agttccgcgg
     7861 ccgctaccgg cgcatcgacc tgctgctcat cgacgacatc cagttcatcg ctgacaagga
     7921 acgcacccag gaagagttct tccacacgtt caatgcgatc cacgaggatg gcaagcagat
     7981 cgtcctctcg tccgaccgac cgcccaagct gatcacgacg ctcgaggagc ggttgcgcag
     8041 caggtttgaa tggggcctga tcgccgacct gacggcgccc gacctcgaga cgcgcatcgc
     8101 catcctgcgc gccaaggcag aggaacagca ggtccacatc ggctcggacg tgctcgagtt
     8161 catcgcgcgc aaggtgatca gcaacatccg cgagctcgag ggcgcgctca atcggatcgt
     8221 ggcctacgcc aacatgggcg gcatgccgct caccaatgac ctggcgcagg ccgtgctgtc
     8281 gaacgttctc tataacccca agcgccggct cgtcacgccc gagcgcatcg tgcacgccgt
     8341 gtccgagtac tacagcgtcg agctcgacgc gctcaagggc cagaagcgcg atcgggcgat
     8401 cgtcatgccg cgccaggtgg cgatgttcct gatccgcgcg gagaccgacg tgtcgctgtt
     8461 gcgcatcggc caggagctgg gcgggcgcga ccactcgacg gtcctgcacg cgtgcgagaa
     8521 gatcgaccgc gagacccagg agaacgagga gctgcggcgc gacctggctg ccgtacgcga
     8581 gctcatctac tccgagtgat tcagtggttg cctgcgggca attgcgggcc gcttgggtgg
     8641 ttgctccggc ggtcctgacg gtggaaacgt gccttacgca cgtgtggata tgggcccgtg
     8701 gataggtgcc tgagcggtgg atcggccgga gtggtgtgga agatcggccg gatcagtcca
     8761 ccgctgttcg atcggacgga gcgatccttc caccgaacac ctggtcggtt ggaggcgatt
     8821 tcacggggga accggggcat atcccccagt tccacaccat gatgatgact acggaatgag
     8881 aagataagaa gagataccaa taatcaggcg atcgtccggt agagggagcg caagccacgt
     8941 gaagctttcc gtgatgcagg agaacctggc gcgcgggctg ggcatcgtca gccgcgccgt
     9001 cagcagccgc agcacattgc cggtgctgtc caacgtcctg ctgcgcaccg aggacgccgg
     9061 tctgaaactg accgcgacca atctcgagat cggcatcacc tgctgggtcg cgggcaagat
     9121 cgatgccgag ggcgccatga cggtgcctgc gcggctgttc aacgatctcg tcgccgggct
     9181 gccggcgggc gagcggatcg atctcgaggc cgacgacgcg acgacgctgc gcatcaaggc
     9241 cggccgctac cagacgcagc tgcgcggcat cgacgccgag gaattcccgg tcatcccgtc
     9301 gcccggtgag cggcccacga cgcgggtaaa ccagaaggag ctgcgccacg cgctgagcga
     9361 ggtcgtgttc gccgcggcca gtgacgaggc gcggcccatc ctgaccggcg tcctcacccg
     9421 cctgtcgggc gacaagctga ccctcgctgc cgcggacaac taccgcatcg ccgtgcgcag
     9481 cctggccatc ctcgatccgg tcgaggacac gagcctggtt gtcccagcgc gctcgtacgc
     9541 cgagctgatg cgcgtcatga ccgacagcga cgatccggtc gagatcatgc tcgcgagctc
     9601 caagagccaa gtgctgttcc acgtcaacga catcgacatc gtcagccggc tgatcgacgg
     9661 ccagttcccg aactaccagc aggtgctgcc gtcgtcgcac tcgacccggg cggtggtgga
     9721 tcgcgatgag ctgctcaagg ccgtgcgcct ttccgcgctc atcgcctcgt ccgcggccaa
     9781 cgtggtgcgg ctgcgcctgg gcgacgacgg gtcgggcacg atcaacgtgg cggccgcggc
     9841 cgacgtcggc gaggcgcagg gcgacgtgga ggccgcgatc gagggcgagg gcgtccagat
     9901 ctcgttcaac gcgcgctacc tgcaggaggc gctccagagc ctcacccagg accagctggc
     9961 gctcgagttc gcgggcccgc tgtcgccagg cgtgctcaag ccgaccgatg acgaggagta
    10021 cgtccacgtc atcatgcccg tccgcactcc ctcctgaaac caccccgcca cgtggaggcg
    10081 ggcaccagaa gatgcgcgta acccagctct cgctcgccgg ctttcgcagc tacgcagcgc
    10141 tgtcggccga ctttccgccc ggaccgcagg tcgtcgtggg cgataacgcc acgggcaaga
    10201 cgaacctgct cgagagcctg gttgtcttgg ggacgggcca ctcgcaccgc gccggcgcgg
    10261 acgctgagct ggtcgagtgg ggcgccgact tcgcgcgcct cgaggcgacg gtgtccggag
    10321 ccgggggatc ggcgacgcag ctcgaagtgg tcatcgccat ggcggcgggc ggcggcgggc
    10381 gcaagcgcgt ccaggtcaac ggcgtcgccc gccgcccaac cgcgctgagc gccgcgttac
    10441 cggtcgtgct cttcgcgccc gaggacatgc tgctcatctc gggcggaccc accctgcgcc
    10501 gcacggcgct cgacacgctc gtcgcccagg ccactcccgg cgcgagcgcg acgctctcca
    10561 cctacgcccg cgcgatcacg cagcgcaaca acctgctgcg cgcgatccgc gagggcacgg
    10621 ccgcgccaga cgagctgcgc ttctgggacg aggtcgtgat cgacgacggg gcgcgcatcg
    10681 tcgactggcg gcgcgatgcc ctggcgcgcc tcgccgagcc gctggctgca gcccatgctg
    10741 aaatcgcgcc agccgaggag cgcctcgagc tgcgctacgc gaccaatgcc gagcggctcg
    10801 ccgcggagtc gacgagtgac gcgctgcgcc gccgcctggc ggacacggcc gagaaggagc
    10861 agtggaacgg cgccacgctc atcggaccgc atcgcgacga catcgtcttc accagctccg
    10921 cgcgcgacct ggccgacttt gcgtcgcgcg gccagcagcg cacggcaatc ctcgcgttca
    10981 agctggccca gctcgacctt ctgcgcagcg cgacgggtga ggcgccgttg cttctgctcg
    11041 acgatgtctt cagtgagctt gacccgagcc ggcgcgccca tctcgtgcgc cgtatcggtg
    11101 agctgcccca ggccttcgtg acgaccacca cgaccgacga tctcgatccc catcttgtcg
    11161 cggccgcgac cgtgtggaac gtcacgcccg gacggctgga gcgcgcggcg tgagcccagt
    11221 gggcgggggt gggctgtgag cccgcgccgg ccgatgaagc gcatcggcga catgctgccc
    11281 gacgttgccg cgggcctggg cattgacgcg gagctgcgcc tggcgcgcca gatggccgcg
    11341 tgggagcgcc tcgtcagtga gcgcgtcccg gccgcgaccg gccggtcgcg cctcctgtcg
    11401 atccagccgc cggcactcgt cgtgagcgcc tcggacccgc gtgtcgggct cgagctgcgc
    11461 atgcgccagg ccgagttgct gactgctttc gcccaggcgc ccgagggcgt tcggctgctc
    11521 gagctgcgcg tcgttgtgcg accgccgggc gccacgaatc cgcctcccgg ccgaagcggc
    11581 gggccacgct agactcactc accgatggca tcccggctac agatcaagta cgggcttgtc
    11641 gccgaggagg atcgcctctc gagctcgtcc gatgcactga tcgtgaccga gccgacgacc
    11701 ggttcgaagg cgcgcaccaa gggcagcctg tacctcatca cgaccagccg ccagaccggc
    11761 ggccgcaccc gcgaggcctg ccggctcgtc gccgattcga tccgtcgtga gtattactac
    11821 gacgaatcgg cgggcatcgc catcgtcctc gagaaggcga ttcgcgcttc gaaccgacgg
    11881 ttgcgccact cgcgcgaggg caatggcctg cccatcggcg cgatcggcat cgcgctcgcg
    11941 gtcgtgcgtg gcaacgagat ctacgtcgcg acctgtgggg aggccgatgg ctacctggtg
    12001 cgctcggccc gactgctcat gccggagcac gactcgggct ccggcctgcc ggccgcggac
    12061 agcctgaagg tcgacgtctg gcggggcgag ttctcggtcg gcgattcgct cctcctgtgc
    12121 tcgcgcaacc tggtcgaggc cgtgggcacg gaagagctca agaacgcggt cgtgaccctc
    12181 cacccccagt ccgccgcgga gcacctgcac cacctgttcg tcgcggccgg cgggacgggc
    12241 tccgacgccg tgctggccat cgaggcaagt gaggtgtcgc tcgccaaggt cgagcaccgg
    12301 ctcgtcccgg tgagcccgtc cgagccgctt gcgggcacgc ccattgcctc accaatcccg
    12361 ctggccgaca agatggcggg tgccgcgtcg gccgtgcagg agcgcgcggt cgcggcacgg
    12421 ggcgcactgc gccagggcgt gggccgcatc gtcgaccacg tgctcgacct gatgccgcag
    12481 cgccgcacct cgttgcggcg catcactgcg gcgaccacgc gacgtgaaac gcagcgccgc
    12541 atagcgctcg ccgtcctggg cttcatcgcg gtcgtcgccg tggtcggggt tggcctgtgg
    12601 tacgcgccgc agcgcggcga ccggccgatc gaccaggtga accagggcga ggcggcactg
    12661 gccgacgcgg tcgaggccgc caacctggtc ttcggcccgg acgacctcct gcactccgac
    12721 cccgacgagg ccttggtgct gctccagggc gcctggtcgg acctggccgt cgcgcgcgaa
    12781 gcgcaggtgg atccgcagac gatcgcccag ctcgacacgc agctgcgctt tggactcgaa
    12841 accctctacg ccacggccac ggtcaccgcc acgcagctgt acgcggcgcc agagggcgcg
    12901 cgggtgtcgg cgctggccca gggaccagac agcgcggcct acgcgatcgt cggcgatacc
    12961 gtcgtccgca tcgatccgga gaccggcgca atgacgacgg tgatcaccgc cggcgacggg
    13021 ctgggcggcg gcatcggggt gccgcgcctg ctcgcgcacg gtgggcccga cctgctgatc
    13081 cttgatgaag acagccgcct ttggcgctgg cggccgtccg gcgcgctggg cgaggtgcgg
    13141 ctgtcgggcg accagaagtg ggacgagacg gtcgtcgact tccaggcgtt catcatcaat
    13201 ctcgaacagg ggctgtaccg gatctacgtg ccctatccca ggacgagtca gatcctgcgc
    13261 tacgacccga ccgcggacgg gggcggcttc tcggcacccg cgccgtactt catcagcgag
    13321 aacgtcgacg tcacccggtt ccgccagctg tacgtcgacg gcgacatcta cgccgtcacc
    13381 tcggagaacc tggagcagta cttcagcggc cgccagacgg ctttcagcct cgacccaccg
    13441 cccgacgacg gcgacctgcg cgccggccac gactatgcgt tgctgagcgc gaccggtgtg
    13501 cgcggcgacg gcgacctgta tgtctgggac aagctgtggt cgcgcgtcct cgtctacgac
    13561 aaggcggcgg gcgcctactc aactcagtat ctcgccgcgg acggcacccc gccactggcc
    13621 gacctcacag gtatgtacgt ggtcgaccgc ggccactccg agccgccgct gctgatcttc
    13681 accacctcga gcggtctctt ccgggtggag ctgggcgccg gcgcggtccc gcaaccgaca
    13741 gccagccccg cggccagcgc ctcgccgccg ccgactctta cgccctcgcc gtacccgact
    13801 gcacccggtg agacgccgtc accggagccc acgcgcccca ggcgcacgcc ccgccccgtc
    13861 gagtcgccga cggccgctcc gtgacctttg gcgcggtcta gaatttcggc catgcccggg
    13921 ggcgcgaacg gccacaacgg cggcctgaac gagatgatcg tcgagagtgt ccgcgtccac
    13981 atgctctcgt cgcagcacgt cgtcatcctg cgcgacacgg gccacgatcg ttacctgccc
    14041 atctggatcg ggccctggga ggcaaacgcg atcgcgatgc gtctgcaggg cgtgaccccg
    14101 gagcggccga tgacccacga cctgttcagc caggcactga ccgagctggg cgtgacgctc
    14161 aagcgcatcg tcgtatcgga cctggcggag gacacgttcc gcgcgcgact gctgctccag
    14221 gtcgacggcc gcgagctcga tcttgacgcg cggccctctg atgccatcgc gctcgcggtc
    14281 cggagcggcg tgccgatctt cgccaccgac gcggtgctgg accgtgccgg ggtcgtgccc
    14341 gagggcgcgg aagaggagaa gctgtcggtc tttcgcgagt tcgtgaactc gctcaacgtc
    14401 gatctgcctg acgaccccga aagcggttcc cgaaaactcg actagggcgg ccgactaggt
    14461 agtcgggccc tcggccagca acggctcgcc gcgatccagc gcccccgggc ggcggaacca
    14521 cagtcgcagc cgcagccgcc agaaatcgcg caccgcgcgc agcacgacct tcgggttggc
    14581 gccggtctga ctgccggcga tgcgcgggta gtgcggcacg ccgacctcgg cgatcgttcg
    14641 gccgcgctgg agcagcttca cgagcagctc acctgacagg aagccaccct gcgattcaac
    14701 gctgatgccg tcgagcgccg cgcggcggaa gagcttgcac gcgcagtcgg ggtcgcgcac
    14761 cttgaggccg tagaagatac gcagcgccaa ccggtaggcg cgcgcgtagg ccagccgaac
    14821 gaacggatca gcacgcttga tcctgtaacc gaccaccacg tctgggttgt cggccgc
//
LOCUS       DATBRX010000168        29271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1851057, whole genome shotgun sequence.
ACCESSION   DATBRX010000168 DATBRX010000000
VERSION     DATBRX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 29271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29271
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851057"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VIF08_06430"
     CDS             <1..767
                     /locus_tag="VIF08_06430"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY8135661.1"
                     /translation="QVPMLRPRRLREQEVVEQVLRAGPHLLVLADYGQIVPAALLTTP
                     RHGALNLHPSLLPRHRGASPVQAAILEGDKETGVTIIQMDSGLDTGPVVAQARYATSG
                     TETAPELETRLSLLAADLLAGTLEPYLAGEITPRPQPAEGVTMTRPLRREDGRLELVR
                     GIAYLDRQIRAYLPWPGTFFETDAGRVIVWDARPLGGGATRETGTLLRLPANRVALAA
                     SDGLLELVEVQPAGGRRMSAAELVRGRPGLVGSVIR"
     gene            808..2016
                     /gene="rlmN"
                     /locus_tag="VIF08_06435"
     CDS             808..2016
                     /gene="rlmN"
                     /locus_tag="VIF08_06435"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY8135662.1"
                     /translation="MLSETVGDGSAQPLISLPPAGARDPRPGLSGVPVAKLEAWLAER
                     GQPAYRARQIADHVWAGAAQSIDELRTLPQPLRRELEESFRLTTLGPPDITPSDGGLT
                     QKALHRLDDGRLIETVLMRYPARGWRKARATACISSQAGCAVGCPFCATGELGFWRDL
                     EVAEIVDQVRVWRQWLAGVGQRLTNVVFMGMGEPLLNVERVLDAAAAITDRARFGLGA
                     RHVTISTSGVLPGIERMIAAGPTYTLAVSLHAARPELRDVLVPLNRRYPIADVVVAAG
                     RYAATTGRRVTYEYVMIEGINDTPADAHAAVSLLRGQLAHINLIPMNPVAHTPWQPSP
                     ASRVEAFAAILREGGLGTTVRRNRGVDIGAACGQLAADRAGAPAPAAVARRQELLVVR
                     SAAAISAARR"
     gene            2050..2739
                     /gene="rpe"
                     /locus_tag="VIF08_06440"
     CDS             2050..2739
                     /gene="rpe"
                     /locus_tag="VIF08_06440"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY8135663.1"
                     /translation="MRKIEVAASITTADFGNLYRVVRKLENAGVDRLHLDVMDGHFVP
                     NITFGPDVVAAVRRLTDLPIDAHLMISEPARYLERFIAAGPDTITFHYEIDDSAHGKH
                     ATLARIRDAGRGAGLAISPSTPAKVLASFADDLSLVIVMTVEPGFGGQHFMPGPAAKI
                     ADVRRMFRDRPRFAVHADGGVSSATAEVVGSHGVTTCIVGSALFQRGRDAALEVQEVK
                     RLAGNARYDAR"
     gene            2739..3203
                     /gene="dtd"
                     /locus_tag="VIF08_06445"
     CDS             2739..3203
                     /gene="dtd"
                     /locus_tag="VIF08_06445"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY8135664.1"
                     /translation="MRLLLQRTTGAQVRVDRKVVGAIGPGLVALVGIGHADDETTVEA
                     LAGKTVDLRIFRDEDGKTNLSLADVGGEVLAISQFTLYADTRKGRRPSFLDAAPPDMG
                     ERLYETYCRAVEARGVRVARGLFGAEMEVELVNDGPMTIWLDSAAGATSPAG"
     gene            complement(3200..3394)
                     /locus_tag="VIF08_06450"
     CDS             complement(3200..3394)
                     /locus_tag="VIF08_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HEY8135665.1"
                     /translation="MARACAICGKTSMAGFNPQSTGMNRVRAHRRMTANLQKMRLPVG
                     GVERSVLVCTRCQRTLQKTA"
     gene            3504..5156
                     /locus_tag="VIF08_06455"
     CDS             3504..5156
                     /locus_tag="VIF08_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEY8135666.1"
                     /translation="MNNMSWDGHDLMAAMRHAGAVLHANLDEVNALNVFPVPDGDTGS
                     NMLATIQAAVAEAESVPPLERTPTRISSALSLGALMGARGNSGVILSQLLRGMGEAIA
                     GHERVTAPILVEAFKRGCTTAFQAVAHPVEGTMLTVARDVAQASEKAPRGDGSIEHLL
                     RVAVDEAAASVQRTPSLLPVLKEAGVVDAGGRGIELLLRGALASIQGDHLPHGMKLTN
                     DIALPTWDALEAEGYGYETVFVILPPEGARLDVAAIRARLDRMAESVLVAGDDRAVKI
                     HVHNERPDEVLGYGLTLGTLSRITIENLDRQATDVRERTRVSTEAASISGAAPLREGP
                     AVIAVAAGDGLARVFSSLGATGVVQGGQGANPSVGELADAIRGSGNGEVIVLPNNPNV
                     RLAAKQAGELCPGVKVEVVPTRNAGEGVAALLAFDPDAALKDAAKQMTQAGRNVQTLQ
                     VSLAVRDARMGRHKVHRGDYIVLGPNDGLVAAHADRTAAIRSGIGELKSGFELLTLYR
                     GRDVDHAAGQELRDQLATELDGVEIELVEGGQPHYDFLIAAE"
     gene            5157..7484
                     /locus_tag="VIF08_06460"
     CDS             5157..7484
                     /locus_tag="VIF08_06460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY8135667.1"
                     /translation="MPEARTAKPAAARASAADAALPILDQDIRAVVAPGMAKNLRRLE
                     LNTVRDLIFHFPRRYDDFTHQMTLREIASQPPDGPVAATVEIVDLRVDPGFRRRVQRT
                     VARVRDASGEGEAVWFGRRYIERRLAPGQTVALAGKVELRGWLPRFANPEFGAADDSA
                     LHAGRIVPVYRLTAGVTAPTLRARIRLALDRAARSQVEYLTPGMGGQLAERLANIEDA
                     LEWVHFPPDFDHRDQALRRLAFDELLALQVGMVARKRQRETELGVPITVADERFRAAQ
                     KQVEEVIATQVRARIAARSPGDDKLEITLTSDQRAALDDIRVDLAGKRPMMRLLQGDV
                     GSGKTAVAALALAFVADAQHQGALLAPTDLLARQHAATLGRLLEPLGHEVVLLTGSLP
                     GGQRADALALLGAPMATIEGRTRGRIAVGTHALVQDAVSFADLRLVVVDEQHRFGVAE
                     REALTSKGAAPHVLLMTATPIPRTLGQIVHADLDVSDLRTPPAGRQQIRTAIRRTHEL
                     VRREDGRQGAIALIAQQVAAGYRAFVVVPLVEEDETTAGRSVEQAAELVREKWPEAAQ
                     VAGVDVAVPEIEIVHGQMKAADRDARMERFRRGEVPIVVGTTVLEVGVDVPEATVMMV
                     LDADRFGLAQLHQLRGRVGRGEAESYCVLVTERYPADPQNPADDEEATVAARLDALVA
                     TTDGFVLAEKDLEQRREGELLGLSQSGLPPLRVASLSGAANQALSLQARTIAERIVDS
                     TGRLPADYEALEHELKVGWLRRVGAGEVLTQDANA"
     gene            7493..8068
                     /gene="rsmD"
                     /locus_tag="VIF08_06465"
     CDS             7493..8068
                     /gene="rsmD"
                     /locus_tag="VIF08_06465"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY8135668.1"
                     /translation="MPDAGRVIGGRARGVRLEGPRGTATRPLTDRVKESLFGALEAEG
                     ALDGSFLDLFAGTGAAGIEALSRGASAATFVEHDGANCQLIGANLRRAKLEGGRVVRA
                     DVVVYLRNLRKETDGPFSAALLDPPYSDPVMVNALELLGDPARDWLVPGATVAVKHFW
                     RDEPADHVGLLAKERVKRFGETAVTFYTRDL"
     gene            8065..8541
                     /gene="coaD"
                     /locus_tag="VIF08_06470"
     CDS             8065..8541
                     /gene="coaD"
                     /locus_tag="VIF08_06470"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY8135669.1"
                     /translation="MTTALYPGSFDPVTFGHLDVIRRATAVFDRLVVGVLVNPRKSPL
                     LGLDERIAALGEALHEDGLDAKVEVAGFDGLTVEYCAQIGASFVVRGLRAVSDFESEL
                     SMAHTNRKLAPDIDTIFFMTALEHAYLSSTLVKEIAAFGGDVSRFVPAAAARRLNR"
     gene            8674..9168
                     /locus_tag="VIF08_06475"
     CDS             8674..9168
                     /locus_tag="VIF08_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135670.1"
                     /translation="MSSNVIVDQARALDLLDQLRVAVPEEIRAAKRINEETERIVERA
                     QEEAERILARAQEQAAFLIEERELTRAAEIRSAEIISDGQREADEIRRGADEYAQSVL
                     VKLEGECIRALQSIKRGLALLDDRHVDEQPGDASPGTPDELAPPGDPERLEPGHSEGT
                     RAQL"
     gene            9155..9694
                     /locus_tag="VIF08_06480"
     CDS             9155..9694
                     /locus_tag="VIF08_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEY8135671.1"
                     /translation="MLSYNVATLLRSAPGTERRYTIEPIELPLYDDLQLAAPIAGEVR
                     LSRTSRSILAKASLSTAIEGSCSRCLKAVVSPISLEIEEEALPSIDIDTGQPVDDSLE
                     PDALRLDDHHELDLDAPIREAISLAEPIALLCRPDCRGLCPVCGVDLNTVTEHGHDDE
                     SIDPRLAALSELRDKIKTN"
     gene            9716..9919
                     /gene="rpmF"
                     /locus_tag="VIF08_06485"
     CDS             9716..9919
                     /gene="rpmF"
                     /locus_tag="VIF08_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY8135672.1"
                     /translation="MGVPKRRVSHARQGDRRAHQALTAPQLEECPHCHAQKRAHHACD
                     NCGWYNGREAVRIKAKAKPEAAS"
     gene            9965..10993
                     /gene="plsX"
                     /locus_tag="VIF08_06490"
     CDS             9965..10993
                     /gene="plsX"
                     /locus_tag="VIF08_06490"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY8135673.1"
                     /translation="MDLEVLRSGHTATPGRVRVAVDAMGGDHAPEEVVIGSVDWARGH
                     PDTDVILVGDEPRIEPFIGPDRPANVSIVQASQSVSMEEHPAAAVRSKRDASINVCMR
                     LVKDGQADAVVTAGHTGAGVASAILFLGRLKGVDRPALAVQMVTDKGPMVLLDIGATT
                     DSSGLNLYQYAHMGTILAEQVFGVVDPSVALLSIGEEGGKGEGRVQEATELIKASPLR
                     FIGNAEGKDLPHHPADVVVCDASVGNVTIKFFEGLSSFIFDMLRTEFKRPPWGPLGYL
                     FMRPGIAQIRKRFDYERLGGAPLLGVKGTVLITHGRARRRMIGFAVEVTAAAARARIP
                     DRIADALA"
     gene            10980..11414
                     /gene="nusB"
                     /locus_tag="VIF08_06495"
     CDS             10980..11414
                     /gene="nusB"
                     /locus_tag="VIF08_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY8135674.1"
                     /translation="MPSHEQQREARRLALGAVFEADFGQHTATTILERRLSEEGTPDD
                     TAELARVIVGAVVERRDEIDAQIGSLAPRYPVVQLAKIDRALLRSAISELLHSAATPP
                     SVAIAEWVELARTYSGEPARRLLNGVLGRVARDRQTQEREHQ"
     gene            11411..11650
                     /gene="acpP"
                     /locus_tag="VIF08_06500"
     CDS             11411..11650
                     /gene="acpP"
                     /locus_tag="VIF08_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8135675.1"
                     /translation="MTAETYERLKKIVVEQLGVDEDQVTPEASFVDDLNADSLDLVEL
                     IMSLEEEFGAEISDEDAEKIRTVQDAVDYVEDKGK"
     gene            11691..12404
                     /gene="rnc"
                     /locus_tag="VIF08_06505"
     CDS             11691..12404
                     /gene="rnc"
                     /locus_tag="VIF08_06505"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY8135676.1"
                     /translation="MTSARDLASRLGLPFSDFTFLEQALVHSSYVHEQPSVGASNERL
                     EFLGDAVISVIVSEALFERYPDDDEGSLTTRRAAIVSARGLSRIAQRLELGSYLLLGL
                     GASSADERSRKSVLAATFEAVCGAVYLEFGLARTKRWLRKVAEPELAAQGAVDDLKPA
                     KSVLQERAYAKTGRAPHYELLSDDGPPHARHYVVEVRIGGLAMGRGEGRSRREAETEA
                     ATLALVALDAEAKQPGSRT"
     gene            12401..16012
                     /gene="smc"
                     /locus_tag="VIF08_06510"
     CDS             12401..16012
                     /gene="smc"
                     /locus_tag="VIF08_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY8135677.1"
                     /translation="MSRRLTALRLVGFKSFAERTTVEFGEGISAVVGPNGSGKSNLAD
                     ALRWTLGEQGRMLRTRRSEDVIFAGSSARRAIGMADVTLVIDNSDRLLPVDFGEVEIG
                     RRLYRSGENEYLLNRQKIRLKDLVELLDAGNLADNAFLFIGQGMVDQALALRPEERRP
                     LFEEAAGVRRHERRRRQAEAELLEAEANLERLRDLLAELRPQAKRLAAQAEQLQARRS
                     AGAELAEALLEAARARWGALSQSAAKEAREVERSRAAADAALAELKAAETEATRLAAE
                     ISRRAEAEQALRRTLDKQREQLTALRLDQARLETEVAAQARDVERVTAEQTAAEARRA
                     AALAELSRPIPEADPALATELDYVERQLAELEDAHADAAGGEPATVDIAARRREAEGH
                     ERGLTTLENSVATARARTAEVEARRLAAVHELADAERGLETASNEEKAAAEDVAAARI
                     AADAALAARAGALARLAEAEADAGAVRGRLAALDAALAALSDEGLAKAARARGGALVA
                     EGLEIEPRFRAAVSAALGAAASGFLVDEGSVTALAGRRGNLAVSGVAAKPTSPAALAQ
                     ITEAAIAAGGGRLSEAIRRDPQSQVTRLLERLVWLPDLAAALNLRAALPQGWRAVTLA
                     GEVVADDGLIQLAATESVLEQRAQRDAQAQQLQEMEATLDSLRAEVKVATDGAAKAAD
                     ALADAGKRHEATRRQLRSAEEIERLAQRRGEQLVREQTWQKSQLERLESETTAQRDLV
                     GRITADIARLEADSGTSAAGAAPRSTVKRAAELRERAAELRRQIASQAGATNAAQEAR
                     RRAEVTLAMDEARLRDLAAERQRTALRDAELTERRAQLMAQLEIATKADAEAAANLEA
                     AYAGGAEERANLTAAETRATTARETLREHETRSRAAEVRALETRLLLEQSREGLLVEL
                     AAIGDDGLAALTRAAGDEPPAEGHEAEPGELEDLLSRTLERWSADKSAPEPVSTGKLS
                     SLRRRFHELGAGNPFAVEEYAELRERLDGLEAQRIDVEAAISSTRDLIASLGALINDQ
                     FRATFAALEDAFARRFTELFGGGEAQLSLTAPDDLSTTGIDIHARPPGKKRQPLQMLS
                     GGERALTAVSLLLAMLEVRPVPFCVLDEVDAALDEANVGRFSRALRGLAEQTQFIVIT
                     HNRGTIEAADALYGVTLGDDAVSRIVSMKLPAPTNGKENGTGNGKHAAPELAETTPT"
     gene            16009..16890
                     /gene="ftsY"
                     /locus_tag="VIF08_06515"
     CDS             16009..16890
                     /gene="ftsY"
                     /locus_tag="VIF08_06515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8135678.1"
                     /translation="MTLEHGVARTRSGFVSRLREALGRPLDDDTWDEIEEALIAADLG
                     AALAARVVEGARARRDLEPEIAVKNELVGLFAPRQTTGWPRIPGGGTPAVVLVVGVNG
                     TGKTTTVAKLGQRFKTLGANVLLAAADTFRAAAIEQLEVWAKRADLPLVAHAAGADPS
                     AVVYDALDAAAARASDVVVIDTAGRLHTKSNLMEELAKIRRTIGKRLPDAQPEVLFVL
                     DATTGQNGLSQARAFHEAAGLTGICLTKLDTTSRGGIAFAIEDALDVPVLFVGVGEKM
                     DDLLEFAPNDFVEALFA"
     gene            complement(16918..18219)
                     /locus_tag="VIF08_06520"
     CDS             complement(16918..18219)
                     /locus_tag="VIF08_06520"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8135679.1"
                     /translation="MAAEPLLDPKFPPEDERPTPALWRNNAFLRVWSAATISIFGSLI
                     TRIALPFVAIVTLGADSVGVALVRSMELIAGLTVGLVAGAWVDRLRRRPVMIWADLGR
                     AGLLGLIPLAAFGGWLSLPLLLLVTLLTAVLTTFFDTADNAFLPTIVPRADLVRANGA
                     LAASGSVSEFAAFGSAGFLVQILTAPVAILVDAFSFIVSALLLGSIRVREAPPPDRSE
                     REPVLREIALGLRLVAGQPVLRALTLASMASASMWGVFGALWYLFAIEDLGLDAAAIG
                     IVAAVGGISSLFGALLTTRLTGRFGVGRVLLVAMLVGMLGSYSIPLAPIGAPLLAMLF
                     LIGQQLITDPAMTAFDITDTSIRQTIVHDRQLGRVNATVRVAILVAQLAATLVAGFLA
                     LQLGLRTMLFIGPTVGLLGVLALWFSPVRKIRRIEDMEPVA"
     gene            18249..19580
                     /gene="ffh"
                     /locus_tag="VIF08_06525"
     CDS             18249..19580
                     /gene="ffh"
                     /locus_tag="VIF08_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8135680.1"
                     /translation="MFDDLSERLRGVLSKLTGRGRITEADVDAAMREVRLALLEADVN
                     FRVVKDFVARVRERAIGQEVLESINAGQMVIKIVHDELTTILGAGDRTFRLAGSPAVV
                     MLVGLQGSGKTTTAAKLARQLIKQGRRPLLVAADPYRPAAADQLQTLGGQIDVPVYRA
                     PAGTKVLDIGRGAVAEAKKSGRDTVLLDTAGRTSLDQPLLDEIGGLAKALEPSETLLV
                     VDAMVGQESVAVAQAFAGAAPVTGLVLTKIDGDARGGAALSISAVTGLAVKFLGTGEK
                     TDALEPFHPDRLAGRILGMGDVLTLVEKAQEHVDQKQAEELARKLQKNTFTLEDFLGQ
                     MQQLKKMGPIGGLIDLIPGMGGAAKEAQAAVERGDMKRVEAIIQSMTVAERRDPDLLS
                     GSRKRRVARGSGTSLQDVNRLLKQFAEMKKLMRQLGGAGGRRGMLPFGPRR"
     gene            19632..21455
                     /locus_tag="VIF08_06530"
     CDS             19632..21455
                     /locus_tag="VIF08_06530"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEY8135681.1"
                     /translation="MSDKEPSSFNDLSGSPDETPTRVEFKPLFSRPASATDQPGPVEP
                     AAAVTPTAAPSSMTMNSRWRWAVVGVATIFVVGLLGAVFILSQPRAGTPSTVARYAPA
                     DTALYMEARLDLPGDQHNLIAQFMSHFPGFADQAAFDQKLDETFESIFESSDSGVSWR
                     DDIKPWFSGEIGVFTARVSPAPGTPPSMTVALRVKEGQKSVLDGWLTPLLGTDWQQST
                     YQDQAIWTGNFGNSLDRVSLAMTDEVLLVSTRIEDLQKALDVGAERSAGMADDAFFLQ
                     QLAALHADRLATFYFDYASMLEGLPSQSLFGSGCFDSATQVATQMKFVGEVRAESDHI
                     SFTERVRVPAVAGAPSQAGYVPSRLAELASADTVLFYEVPQLGANIKYFVGELLTCAE
                     ASGGMPDLSSIETILGTPLPDYFDFVRDAALVVTADGGTYGGGLVASVDDEAIAQSRV
                     AKLIAYVRLAAGSMGEFGPQITFEEQQHGNATITVISVSDLPPDVPFSSLAFTVANGH
                     LIIGLDDFVANTLDQTVDGSLARTQRFAAGVAAGGTNNYPAAFVDISAIRASFEALIP
                     ASERPRYDLEIKPYVEPFDYLMIVGGSQGDVGISNVFLYVK"
     gene            21496..21753
                     /gene="rpsP"
                     /locus_tag="VIF08_06535"
     CDS             21496..21753
                     /gene="rpsP"
                     /locus_tag="VIF08_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8135682.1"
                     /translation="MAVRIRLNRVGATKQPTYRFVVTESRNARNGRSLDTIGHYNPRT
                     DPIEIKVDEDKARDWMKRGALPSATVERLFRRVGVLPAKGE"
     gene            21758..21991
                     /locus_tag="VIF08_06540"
     CDS             21758..21991
                     /locus_tag="VIF08_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY8135683.1"
                     /translation="MSAESLVEYVAKALVDEPDAVEVKAVDDQDGRVIELHVAETDMG
                     KVIGRNGSVAKAMRTLLKVVAAREGESISLEIV"
     gene            22005..22508
                     /gene="rimM"
                     /locus_tag="VIF08_06545"
     CDS             22005..22508
                     /gene="rimM"
                     /locus_tag="VIF08_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY8135684.1"
                     /translation="MNDDRLAVGLVRGVHGLRGAVRVEILSDNASRFEVGSVLYPEGG
                     DRPLTVASAHRDGPGLLVTFREVKDRPTADTLREKYLEAPSDRTALPADTWYWHEIVG
                     CAVATESGAELGTVREVFRVAESEVYVVVGDRGEILVPAVSAVITKLDPPNRQIVVKG
                     AALGITE"
     gene            22505..23182
                     /gene="trmD"
                     /locus_tag="VIF08_06550"
     CDS             22505..23182
                     /gene="trmD"
                     /locus_tag="VIF08_06550"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8135685.1"
                     /translation="MSALRIDVVTIFPELFPGPLAASIPGRALERGLATLTAHDLREW
                     GLGRHRSVDDYPYGGGVGMIMRPEPIHAALAALADESTTKILLDPGGAPFNHELARDL
                     AEREHLLFVCPRYEGVDHRVRSMVDLEISIGDYVVSGGELPAMVVIDAVLRLLPGAID
                     SESPIDESFASGGLEYPQYTRPPEFEGMGVPDVLVSGHHAQIEQWKRDQARSRTREQR
                     PDLLADD"
     gene            complement(23179..23580)
                     /locus_tag="VIF08_06555"
     CDS             complement(23179..23580)
                     /locus_tag="VIF08_06555"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8135686.1"
                     /translation="MLVVGSIVIRVKNLPRQIEFWSRVLDYVPRDDDDDTFALLRPRD
                     GTGPNVSLDTMRSELQIPPRIHLDLYAEDQAAEVKRLKSLGATEIDWPKKAADADFVI
                     MADPEGNRFCVIDSAGWPGFEGRSASSRASG"
     gene            23679..24026
                     /gene="rplS"
                     /locus_tag="VIF08_06560"
     CDS             23679..24026
                     /gene="rplS"
                     /locus_tag="VIF08_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8135687.1"
                     /translation="MSVLDEITAEHLRTDLPELSSGDTVKVSARVREGNRERIQIFEG
                     TVMRLRGGGIARSITVRAIRSGVGVERTFKVHSPRIEKIEVVRHGVARRAQLYFLRKR
                     VGKAASLRERRPS"
     gene            24076..24798
                     /locus_tag="VIF08_06565"
     CDS             24076..24798
                     /locus_tag="VIF08_06565"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY8135688.1"
                     /translation="MGVFNPTRRLENQLQREHGYRIILGLDEVGVAPSCGPVVACALA
                     MPLDKRFISGVRDSKMLSAAQREKLARKILRQALTVGLGAASVREIDQLNIYYATILA
                     MKRAVTRAGAHDFVIVDGRHIRGFEEHAGPYKAVIDGDARVYAISCASIIAKVTRDRL
                     MHKLAARYPGYGWEHNAGYATPDHRAGMSALGVTPHHRRSFITTQRVLAGEQTSFDLL
                     DELGPQTLPELEPQSDLVPISA"
     gene            24819..25658
                     /locus_tag="VIF08_06570"
     CDS             24819..25658
                     /locus_tag="VIF08_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135689.1"
                     /translation="MTIVLGYLAAAAVATVGVWLVAGRNFVSQRDPAVNLADLGAKQV
                     GLMSGLAGYAVTGMVLLVTLGRSISDTSSTAYTTVVTMFFVSWMAYAATAFLFANMSD
                     HDEEARRQPRHGFDAPAVQFAGAAGTLEFAFGLGWLALRPLFLSFGLTRLADLVALIL
                     VGVALASYGLVAHQLHRSGYGPARVLTAIPLLTIAATLVFALVVGALGLRSEQSALDL
                     IVVGFALGAVAFGFFIAVNVLGTYGPTARFLARSAGYLVLAFIQANVVLVAFLLLAVL
                     GLA"
     gene            25697..26059
                     /locus_tag="VIF08_06575"
     CDS             25697..26059
                     /locus_tag="VIF08_06575"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY8135690.1"
                     /translation="MKTARQQAGRAAEDEVAAHLALSGWRLLGRNIVVGRDEIDILAI
                     DPGPPAELVFVEVRSATSDRFGTPEERVDRDKVAHLYRASRALSGPYGLRRRVDLVVV
                     DRRTGRRALRHLRALEPA"
     gene            26247..27266
                     /gene="rpsB"
                     /locus_tag="VIF08_06580"
     CDS             26247..27266
                     /gene="rpsB"
                     /locus_tag="VIF08_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8135691.1"
                     /translation="MPNVSMRQLLEAGVHFGHQTRRWNPKMKRYIFAERNGIHIIDLA
                     KTVKGLEEALEFVRQTVAQGDTILFVGTKKQAQEPIAEEATRAEMPYVNQRWLGGMLT
                     NFVTIRKRLGLLDQLEAQQQNGEFERLTKKEASVKTETMTKLLRTLGGIRKMRRLPGA
                     MFVIDPQREHIAVIEARKLEIPVIGTGDTNVDPDMLDYVIPANDDAIRAIRLLCSLIS
                     DAAVEGGHERAARPVEEPVAIVEEHEEASDEMVAALAAGGTFSFEPEPDEEELLPADR
                     AVEAEEAEEEAEPEPARAAPARPAARAGAARTGAARPGAPRTGTRRPAASKDKAKPAD
                     EEDAG"
     gene            27311..27910
                     /gene="tsf"
                     /locus_tag="VIF08_06585"
     CDS             27311..27910
                     /gene="tsf"
                     /locus_tag="VIF08_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY8135692.1"
                     /translation="MADISAQKVKELRERTGAGMMECKSALVEADGDVEKAIALIRER
                     GQATAAKKAGRDAREGVVASYIHAGGRLGVLIEVNSETDFVARNDEFQKLVKELAMQV
                     AGLDPKYPTIESIPADVLEAKKAELLAGEEVQKKPENIRPQIIEGQLKKWYQSVVLYE
                     QPFRDTDQKVGDLITEAIAKIGENIRVRRFVRYQLGEDI"
     gene            27942..28697
                     /gene="pyrH"
                     /locus_tag="VIF08_06590"
     CDS             27942..28697
                     /gene="pyrH"
                     /locus_tag="VIF08_06590"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8135693.1"
                     /translation="MTSSVADAPSAVRYGRILLKISGEALLGQRPYGVDPGMCTFIAQ
                     QVKEVHGLGVQVAVVVGGGNIFRGMAAAASGMDRATGDYIGMLATIMNGLALQDALEK
                     AGVPTRVMSAIAMNEVCEPYIRRRAVHHLEKGLVTILVAGTGNPYFTTDTAAALRAVE
                     IGAEVLLKATKVDGVYDSDPVTNPAAVRHERLGYEQMLTDKLQVIDATAVTLCMENNL
                     PIVVFDMNKPGHIRAAALGGSIGTVIDNGVGHD"
     gene            28690..29250
                     /gene="frr"
                     /locus_tag="VIF08_06595"
     CDS             28690..29250
                     /gene="frr"
                     /locus_tag="VIF08_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY8135694.1"
                     /translation="MTDDIRRDADARMSRAIEALEREMAAIRTGRAATALVERIQVEY
                     YGTQTPLNQLASISVPEAHLIVIQPWDRSALGAIEKAILKSEVGLTPNVDGTVVRLAI
                     PALTEERRKDLVKLVHKRMEEAKVEIRNHRRDVADEIKKAVRDGDIGADEERREMELL
                     QKVTDRWTGEVDRVGKHKEQEILEVG"
BASE COUNT         4779 a         9994 c        10247 g         4251 t
ORIGIN      
        1 cgcaggtgcc catgctgcgg ccacgccgcc tccgcgagca ggaggtagtc gagcaggtgc
       61 tccgtgccgg cccccatttg ctggtgctgg ccgactacgg ccagatcgtt cctgccgcgc
      121 tgctgacgac gccgcgccac ggggccctga acctccatcc gtcactgctg ccgcgccatc
      181 gcggcgcgtc gccggtccag gcggcgatcc tggagggcga caaggagacg ggcgtgacca
      241 tcatccagat ggactctggc ctggacacag gccccgtcgt tgcccaggcg cgctatgcga
      301 caagcgggac ggaaaccgcg cccgagctgg agacgcgcct gagcctgctg gcggccgatc
      361 tgctggccgg cacgctcgag ccctacctcg ccggcgagat cacgccgcgg ccgcagccag
      421 ccgaaggagt gacgatgacg cgtccgctgc ggcgcgagga tggccggctc gagctggtgc
      481 gcggcatcgc atacctcgac cggcagatcc gcgcctacct gccgtggccg ggcacgttct
      541 tcgagaccga cgcgggacgg gtcatcgtct gggacgcccg accgctgggc gggggggcta
      601 cccgcgagac gggcacactt ctcaggctgc cggccaatcg ggtcgcactg gcggcgtccg
      661 acggattgct cgagctggtc gaggtgcagc ccgcgggtgg ccggcgaatg tccgccgcgg
      721 agctggtgcg tggccggccg ggcctggtcg gatcggtcat ccgatgagct cccggcccat
      781 ccgtcggttc ggggctagca ttcgttgatg ttgtcggaga ccgttggtga cggctctgcc
      841 caacctctga tcagcctgcc acctgccggc gcgcgcgatc cgcgacccgg actgtcgggc
      901 gtgcccgtgg cgaagctcga agcgtggttg gccgaacgcg gccagccagc ctaccgggcg
      961 cgccagatcg cggatcacgt ctgggcgggc gcggcgcaat ccatcgacga gctgcggacg
     1021 ttgccccagc cgttgcggcg tgagctcgag gagtcgttcc gcctcacgac gctcggtccg
     1081 cccgacatca cgccgtccga tggcggcctg acccagaagg cgctccaccg gcttgacgac
     1141 ggccgattaa tcgagacggt cctcatgcgc taccccgcgc gcggctggcg caaggcgcgc
     1201 gcgacggcgt gcatctccag ccaggcgggc tgcgccgtcg gctgcccgtt ctgcgcgacc
     1261 ggcgagctgg gcttctggcg cgacctggaa gtggcggaga tcgttgacca ggtacgcgtc
     1321 tggcgccagt ggctcgccgg cgtcggccag cgactgacga acgtggtgtt catgggcatg
     1381 ggcgagcccc tgctcaatgt cgagcgtgtg ctcgatgccg ccgccgcgat caccgaccgg
     1441 gcgcgctttg gcctgggggc gcgccacgta acgatcagca cgagcggcgt gctgccgggc
     1501 atcgagcgca tgatcgcggc cggtcccacg tacacgctgg cggtctcgct ccacgccgcg
     1561 cggccggagc tgcgcgacgt cctcgtgccg ctcaaccggc gctacccgat agccgatgtc
     1621 gtcgtcgcgg ccggcaggta tgccgccacg acgggccggc gcgtcaccta tgagtacgtg
     1681 atgatcgagg gcatcaacga cacgccggcc gacgcgcacg cggcggtgag cctgctgcgc
     1741 ggccagctcg cgcatatcaa cctcatcccg atgaacccgg ttgcgcacac gccctggcag
     1801 cccagcccgg cgtcgcgcgt cgaggccttc gcggcgatcc tgcgcgaagg cggcctgggc
     1861 acgaccgtgc gccgcaaccg tggagtggac attggtgcgg cgtgtggcca gctcgcggcc
     1921 gatcgcgccg gtgccccggc gccggccgcg gtcgcacgac ggcaggagct gctcgtggtg
     1981 cgcagcgccg cggcgatcag cgccgcgcgc cgctaacaat cgcgcaaagt ccgcgacact
     2041 agggcggcag tgcgcaagat cgaggtcgcg gccagtatca cgacggccga cttcggcaac
     2101 ctctaccgcg tcgtgcgcaa gctcgagaac gcgggcgtgg accgcctgca tctcgatgtg
     2161 atggacggtc acttcgtgcc caacatcact tttgggccgg atgtcgtggc cgcggtgcga
     2221 cggctgacgg acctgccgat cgacgcccac ctgatgatct cggagccggc gcgctacctg
     2281 gagcgcttca tcgccgcagg ccccgacacg atcacgttcc actacgagat cgacgactcc
     2341 gcgcatggca agcacgccac gctggcgcgc atccgcgacg ccggccgcgg cgcgggcctc
     2401 gcgatcagcc cgagcacgcc agccaaggtc ctcgccagct tcgccgacga tctgtcgttg
     2461 gtcatcgtga tgaccgttga gcctggtttc ggtggccagc atttcatgcc cggacccgcg
     2521 gccaagatcg ccgatgtgcg gcgcatgttt cgcgatcgcc cgcgctttgc ggtccacgcc
     2581 gatggcggcg tcagctccgc caccgcggaa gtcgtcggca gccacggcgt caccacgtgc
     2641 atcgtgggct cggcgctttt ccagcgcggc cgcgacgccg cgctcgaggt ccaggaagtc
     2701 aagcgcctgg ccggcaacgc gcgctacgac gcacgctgat gcgtctgctg ctgcagcgca
     2761 cgaccggcgc ccaggtcagg gttgatcgca aggtggtcgg cgccattggg cccggcttgg
     2821 tcgccctggt gggcatcggc catgccgatg atgaaaccac ggtcgaggcg ctcgccggca
     2881 agacggttga tctgcggatc tttcgcgacg aggacggcaa gaccaacctg tcgctcgccg
     2941 atgtcggcgg cgaggtcctg gcgatcagcc agttcacgct ctacgcggat acgcgcaagg
     3001 gccgccggcc gtcattcctg gacgccgcac cgccggacat gggggagcgg ctgtacgaga
     3061 cctattgcag ggccgtggag gcgcgcggcg tcagggtggc gaggggcctg ttcggtgctg
     3121 agatggaagt cgagctggtg aacgatggcc cgatgaccat ctggcttgac agcgccgcag
     3181 gtgcaaccag ccctgcgggc taggcggtct tttggagcgt cctctggcag cgcgtgcaaa
     3241 ccagcacgct gcgctcgacg ccgcccaccg gcaggcgcat cttctgcagg ttggcagtca
     3301 tgcggcgatg ggcgcggacg cggttcatac cggtcgactg ggggttgaaa ccagccattg
     3361 aggtcttgcc gcagattgca caggcgcggg ccattgtctg aaagaactcc gatcgttggg
     3421 atgcggccgc ccggcggggc gttccggctg cgtactatag ctgacgtcgt gcgatcgtca
     3481 tacggcgtcg ccgccgtcca cccatgaaca acatgtcgtg ggacggccat gacctcatgg
     3541 ccgcgatgcg tcatgccggc gccgtccttc acgccaacct cgacgaggtc aacgcgctca
     3601 acgtgttccc cgtcccggac ggggacacgg gttccaacat gctcgccacc atccaggccg
     3661 ccgtggctga ggcggagagc gtgccgccgc tggagcgcac cccgacgcgc atctcatcgg
     3721 cgctcagcct gggcgcgctg atgggcgcgc gcggcaacag cggcgtgatc ctctcgcagc
     3781 tgctgcgcgg catgggcgag gcgatcgccg gccatgagcg cgttaccgcg ccgatactcg
     3841 tcgaggcctt caagcgcggc tgcacgaccg cgttccaggc ggtggcgcac cccgtcgagg
     3901 ggacgatgct gacggtggcg cgcgacgtgg cccaggcaag cgagaaagcg ccgcgcggcg
     3961 acggctccat cgagcacctg ctgcgcgtcg cggtcgacga ggcagccgcg tcagtccagc
     4021 gcacgccgtc gctcctgccg gtgctaaagg aggccggcgt ggtcgatgcc ggcggccgcg
     4081 gtatcgagct gctgctgcgt ggcgccctgg cgtccattca gggcgatcac ctgccgcacg
     4141 gcatgaagct cacgaacgac atcgccctgc ccacgtggga cgcgctcgaa gcggagggct
     4201 acggctacga gacggtcttc gtgatcctgc caccggaggg cgcgcggcta gacgttgccg
     4261 ccatccgggc gcggctcgat cgcatggccg agtccgtcct ggtcgcgggc gacgatcgcg
     4321 cggtcaagat ccatgtccac aacgagcggc ccgacgaggt gctcggctac gggctgacct
     4381 tgggcacgct cagccgcatc acgatcgaga acctcgatcg ccaggcgacc gacgtgcgcg
     4441 agcgcacgcg cgtttcgacc gaggcggcat cgatcagcgg cgccgcgccg ctgcgcgagg
     4501 gcccggcggt gatcgccgtc gccgcgggcg acggcctcgc gcgcgttttc tcatcgctgg
     4561 gcgcgaccgg cgtcgtccag ggtgggcagg gagccaatcc gtcggtcggt gagctggccg
     4621 atgccatccg tggttcgggc aacggcgagg tgatcgttct gcccaacaac cccaacgtgc
     4681 ggctggccgc gaaacaggcg ggcgagctgt gccccggggt gaaggtcgag gtcgtgccga
     4741 cgcgcaacgc gggcgagggc gtggcagcgc tgctggcctt cgatcccgac gcggcgctga
     4801 aggatgcggc caagcagatg acccaggccg ggcgcaacgt ccagacgctg caggtctcac
     4861 tggcggtgcg cgacgcgcgc atggggcggc acaaagtgca ccgcggcgac tacatcgtgc
     4921 tcggcccaaa tgatgggctt gttgctgccc atgccgatcg aacggcggcc atccgttccg
     4981 gcatcggcga gctgaagtcc ggcttcgagc tgctgaccct gtatcgcggt cgcgatgttg
     5041 accatgcggc aggccaggag ctgcgcgacc agctcgcgac ggagcttgac ggcgttgaga
     5101 tcgagctggt cgagggcggc cagccgcact acgacttcct gatcgcggcc gagtaggtgc
     5161 ccgaagcccg gaccgccaag cccgccgctg ctcgcgcatc ggccgcagac gcggctctgc
     5221 ccatcctgga ccaggacatc cgcgctgtcg tggcgcccgg gatggcgaag aatctgcggc
     5281 gtctcgagct caacaccgtg cgggacctga tcttccactt cccgcgccgc tacgacgact
     5341 tcacccacca gatgacgctg cgcgagatcg cgtcacagcc gcccgacggg ccggtcgctg
     5401 caactgtcga gatcgtcgat ctgcgcgtcg atccgggatt ccggcggcgt gtccagagaa
     5461 ccgtggcgcg ggtgcgcgac gcgagcggcg aaggcgaggc cgtctggttc ggccggcgct
     5521 acatcgagcg ccgcctcgcg cccggtcaga ccgttgcgct cgcgggcaag gtcgagctgc
     5581 gcggctggct gccgcgcttt gccaacccgg agttcggcgc ggccgacgat tcggcgttgc
     5641 acgccggccg catcgtgccc gtctaccggc tgacggccgg cgtcaccgcg ccaacgttgc
     5701 gcgcccggat caggctggcg ctcgatcgcg ccgccaggtc gcaggtggag tacctgacgc
     5761 cggggatggg gggccagctg gccgagcggc tcgccaacat tgaggacgcg ctcgagtggg
     5821 tccacttccc gcccgatttc gaccaccgcg accaggcatt gcgtcgtctt gcgttcgatg
     5881 agctgttggc gctccaggtg ggaatggtgg cacgcaaacg ccagcgcgag accgagcttg
     5941 gtgtcccgat caccgtggcc gatgagcgct tccgcgccgc gcagaagcag gtcgaggagg
     6001 tgattgccac ccaggttcgg gcgcgcatcg cggcacgcag cccgggcgac gacaagctcg
     6061 agatcaccct cacgtctgat caacgcgcgg ctctggacga catccgggtc gacctggccg
     6121 gcaagcggcc gatgatgcgg ttgctgcagg gtgacgtcgg gtcgggcaag accgccgtgg
     6181 cggcgctcgc gctcgccttc gtggccgacg cccagcacca gggagcgctg ctcgcgccca
     6241 ccgacctgct ggcgcgccag cacgccgcga cgctgggcag gttgctcgag ccgctgggcc
     6301 acgaggtggt gctgctgact ggctcgctgc ccggggggca acgcgccgac gcgctcgcac
     6361 tgttgggcgc gcctatggcc acgatcgagg gccggacacg cggtcgcatc gccgtcggca
     6421 cgcacgcgct ggtgcaggac gcggtttcct tcgcggacct gcgcctggtc gtcgtcgatg
     6481 agcagcaccg tttcggcgtg gcggagcggg aagcgctgac cagcaagggc gcggcgccgc
     6541 atgtcctgct catgaccgcg acgcccatcc cgcgcacgct gggccagatc gtccatgccg
     6601 acctcgacgt atcggacctg cgcaccccgc cggccgggcg gcagcagatt cggaccgcca
     6661 tccggcggac gcacgagctg gtgcggcgcg aggacggtcg ccagggcgcg atcgcattga
     6721 tcgcgcagca ggtggctgcg ggctaccggg cgttcgtggt cgtacccctg gtggaggagg
     6781 acgagaccac ggctggtcgg tcagtcgagc aggcggcgga gctggtgcgc gagaaatggc
     6841 ccgaggcagc gcaggtggcc ggagtggacg tggcggtgcc tgagatcgag atcgtgcacg
     6901 gccagatgaa ggccgccgac cgcgacgcgc gcatggagcg cttccgtcgc ggcgaggtgc
     6961 cgattgtggt cggcacgacg gtgctcgaag tgggtgtaga cgtgccggag gcgacggtca
     7021 tgatggttct cgacgcggat cggtttggcc tcgcccagct ccaccagctg cgcggacgtg
     7081 tcggccgggg cgaggcagag tcgtactgtg ttctcgtcac cgagcgctat cccgcggatc
     7141 cccagaaccc ggccgatgac gaggaggcaa ccgtggccgc gcggctcgac gcactcgtcg
     7201 ccacgaccga cggcttcgtg ctcgcagaga aggacctgga gcagcgccgt gaaggcgagc
     7261 tgctcgggct gtcgcagagc ggcctgccgc cgctgcgcgt cgcgtcactg tccggcgcgg
     7321 ccaatcaggc gctctcactc caggcacgga cgatcgccga gcgcatcgtc gactcgactg
     7381 gccgcctgcc ggccgactac gaggcattgg agcacgagct caaggtcggc tggctcaggc
     7441 gggttggcgc cggcgaagtg ctcacccagg acgccaatgc ctgaattgtc cgatgcctga
     7501 cgccgggcgg gtcatcggcg gcagggcacg cggcgtgcgc ctggaaggcc cgcgcggcac
     7561 ggccacgcgg ccgctcaccg acagggtgaa ggagtcgctt ttcggcgcac ttgaagccga
     7621 gggcgcgctc gatggatcat tcctcgatct attcgcgggg accggtgccg cgggcatcga
     7681 ggcgctcagc cgcggcgcga gtgcggcaac cttcgtcgag cacgacgggg ccaactgcca
     7741 gctcatcgga gcgaacctgc ggcgcgcgaa gctcgagggc ggccgtgtgg tgcgcgccga
     7801 tgtcgtcgtc tacctgcgca acctgcgcaa ggaaaccgat gggcccttca gcgctgcgct
     7861 gctcgacccg ccctacagcg atcccgtgat ggtcaacgct ttagagctgc tcggcgaccc
     7921 cgcgcgcgac tggctcgtac cgggtgcgac ggtcgcggtc aagcacttct ggcgcgacga
     7981 gccggccgac cacgttggcc tcctcgccaa ggagcgcgtg aagcgcttcg gcgagactgc
     8041 agtcaccttc tacactcgcg acctatgaca accgcgctct acccgggctc gttcgatcca
     8101 gtcacgttcg gccacctcga cgtcatccgc cgcgcgacgg cagtcttcga caggctcgtc
     8161 gtgggcgtgc tcgtgaatcc gcgcaagtcg ccgctgctcg gcctggatga gcgcatcgcg
     8221 gccctgggtg aggcgctcca cgaagacggg ctcgacgcca aggtggaggt ggcgggcttc
     8281 gacggcctga cggtcgagta ctgcgcccag atcggggcca gcttcgtcgt gcgcgggctg
     8341 cgcgctgtca gcgacttcga atcggagctg tcgatggctc acaccaatcg caagctcgcg
     8401 cccgatatcg acacgatctt cttcatgacc gcgctcgagc atgcctacct gagctcgacc
     8461 ctggtcaagg agatcgccgc cttcggtggc gacgtgagcc ggttcgtgcc cgccgccgcg
     8521 gcccgccgtt tgaaccgcta gcccgtacaa ttcagcgctc agggcgagcg ccgccgacgc
     8581 cgcccgcggc gcatctttgg ggaaactccg atcgacatca tctttctcat cgagcagctg
     8641 gagcggctga tctccggcgg ccgcaacctt ccgttgagca gcaacgtgat cgtcgaccag
     8701 gcgcgcgcgc tcgacctgct cgaccagctg cgcgttgccg tgccggagga gattcgcgct
     8761 gccaagcgga tcaacgaaga gaccgagcgc atcgtcgagc gcgcccagga ggaggccgag
     8821 cgcatcctgg cccgtgccca ggagcaggcc gcgttcctca tcgaagagcg cgagctcacc
     8881 cgcgcggctg agatccgctc ggccgagatc atcagcgacg gccagcgcga ggcagacgag
     8941 atccggcgcg gcgccgacga gtatgcgcag agcgtcctgg tcaagctgga gggcgagtgc
     9001 atccgcgccc tgcagagcat caaacgcggc ctggcgctgc tcgacgaccg gcatgtcgat
     9061 gaacagccgg gcgacgcttc gcccggcacc cccgacgaac tcgcgccgcc cggtgacccc
     9121 gagcggctcg agcccggcca ctccgagggc acccgtgctc agctataacg tcgcgaccct
     9181 gctgcgctcg gcgccgggga cggagcggcg ctacacgatc gagccgatcg agctgccgct
     9241 gtacgacgac ctgcagctgg ccgcaccgat tgccggcgag gtccgcttgt cgcgcaccag
     9301 ccgcagcatc ctggccaagg cgagcctgtc cacggccatc gagggctcgt gcagccgttg
     9361 cctgaaggcg gttgtctcgc ccatctcgct cgagatcgag gaggaggcgc tgccgtcgat
     9421 cgatatcgac accggccagc cggtggatga ttcgctggag cccgacgcgc tgcgcctgga
     9481 cgaccaccac gagctcgacc tggacgcgcc gattcgggag gcgatcagcc ttgctgagcc
     9541 gattgcgcta ctctgccggc ccgactgccg cggcctctgt ccggtgtgcg gcgtcgatct
     9601 caacaccgtc accgagcacg gacacgacga cgagtcaatc gacccgcggc tggccgcgct
     9661 gtccgagctg cgcgacaaga tcaagaccaa ctagtgaccg aggagtagcc acgaaatggg
     9721 agttcccaag cggcgtgtgt cgcacgcccg ccagggtgac cggcgcgcgc atcaggcgct
     9781 cacagcgccg cagctcgagg agtgcccgca ctgccacgcc cagaagcgcg cccaccacgc
     9841 gtgcgacaac tgcggctggt acaacggccg cgaggctgtg cgcatcaagg ccaaggccaa
     9901 gcctgaggcc gcgtcctagc gcgcacccgc cccacgcgcg gctgcggcac caggacacat
     9961 agcagtggac ctcgaggtcc ttcgctccgg ccacacggcc acgcccggcc gcgttcgcgt
    10021 cgcagttgac gccatgggcg gtgatcacgc cccggaagag gtcgtgatcg gcagcgtcga
    10081 ctgggcgcgc ggacacccgg acactgacgt catcctcgtt ggcgacgagc ccaggatcga
    10141 gccctttatc ggtccggaca ggccagccaa cgtcagcatc gtgcaggctt ctcaatcggt
    10201 gtcgatggaa gagcaccccg cggccgcggt gcgcagcaag cgcgacgcgt cgatcaacgt
    10261 gtgcatgcgt ctcgtcaagg acggccaggc cgatgccgtg gtcaccgccg gccacaccgg
    10321 cgcgggcgtt gcgtcggcga tcctcttcct gggccgcctc aagggcgtcg atcggcccgc
    10381 gctggcagtt cagatggtca ccgacaaggg gcccatggtg ctgctcgaca tcggcgccac
    10441 gaccgactcc agcgggctga acctgtacca gtacgcgcac atgggcacga tcctggccga
    10501 gcaggtgttc ggtgtggtcg atccgtcggt cgccctgctg tccatcggcg aggagggcgg
    10561 caagggcgag gggcgcgtcc aggaggcgac cgagctcatc aaggccagcc cattgcgctt
    10621 catcggcaat gccgagggca aggacctgcc ccaccacccg gccgacgtcg tggtctgcga
    10681 cgcgtccgtc ggtaacgtca ccatcaagtt cttcgagggc ctgtcgagct tcatcttcga
    10741 catgctccgg acagagttca aaaggccgcc gtgggggcca ctgggctacc tcttcatgcg
    10801 ccccggcatc gcacagatac gcaagcgctt cgattacgag cgcctgggtg gcgcaccgct
    10861 gctcggggtg aaggggacgg tgctcataac ccatggccgg gcgcggcggc ggatgatcgg
    10921 cttcgcggtc gaggtgaccg ccgccgccgc acgtgcgcgc atcccggatc ggatcgccga
    10981 tgccctcgca tgagcaacag cgcgaggccc gccggctggc attaggggcg gtgttcgagg
    11041 ccgatttcgg tcagcacacg gcgaccacga tcctcgagcg gcggctgagc gaggagggga
    11101 caccggacga caccgcggag ctggcgcgcg tcatcgtcgg cgcggtcgtg gaacgacgcg
    11161 acgagatcga tgcccagatc gggtcgctgg cgccgcgcta cccggtcgtg cagctcgcaa
    11221 aaatcgaccg cgcgctgttg cgttcggcca tatctgaact gctacactcg gccgcgacgc
    11281 cgccatctgt ggccatcgca gagtgggtcg aactcgctcg cacatacagt ggcgaaccgg
    11341 cccgccggct gctcaacgga gtgctcgggc gagtcgccag agaccgccaa acacaggaga
    11401 gggagcacca gtgaccgctg agacctacga gcgccttaag aagatcgtcg tcgagcagct
    11461 cggcgtcgat gaggaccagg taacccccga ggcctcgttc gtcgacgacc tgaacgccga
    11521 ctcgctcgac ctcgtcgagc tgatcatgag cctggaggag gagttcggtg ccgagatctc
    11581 ggacgaagac gccgagaaga ttcgcaccgt tcaggacgcc gtcgactacg tcgaggacaa
    11641 gggcaagtaa cgacccgtcc cgggcgggtc gcacggagct gccagacaca ttgacctcgg
    11701 cgcgcgatct cgcgagccgg ctcggccttc ccttcagcga cttcacgttc ctcgagcaag
    11761 cgctggtcca cagcagctac gtccatgagc aaccctccgt tggcgcgtcc aacgagcgac
    11821 tggagttcct gggcgacgcg gtcatctcgg tgatcgtctc ggaggcgctg ttcgagcgct
    11881 accccgatga cgacgaaggc tccctgacca cccgccgcgc ggcgatcgtc tcggcacgcg
    11941 gcctgtcgcg catcgcccag cgcctcgagc tcggttcgta cctgcttttg ggactgggcg
    12001 cttcgtcggc cgacgagcgc agccgcaaat ccgtcctcgc cgcgacgttc gaggcggtgt
    12061 gtggcgcggt ctacctggag ttcgggctgg cgcgcacgaa gcgctggctg cgcaaggtgg
    12121 cggagccgga gctcgccgcg cagggcgcgg tggatgacct gaagccggcc aagagcgtcc
    12181 tccaggagcg cgcctacgcc aagaccggtc gagccccaca ttacgagctg ctcagtgacg
    12241 acggcccgcc ccatgcgcgg cactacgtcg tcgaggtgcg catcggcggg ctggccatgg
    12301 gtcgcggcga gggccggagc aggcgcgagg cggagactga ggcggcgacg cttgccctgg
    12361 tggcgctcga cgctgaggca aagcagcctg ggagccgcac atgagccgcc gcttgacagc
    12421 gctgcgcctc gtgggcttca agtcgttcgc cgaacggacc acggttgagt tcggcgaggg
    12481 catcagtgcc gtggtcggcc ccaatggcag cggcaagagc aatctcgccg acgccctgcg
    12541 ctggacgctc ggtgagcagg gccggatgct gcgcacgcgg cgctcggagg acgtcatctt
    12601 cgccggctcg tcggcgcgcc gggcgatcgg catggccgac gtcacgctcg tcatcgacaa
    12661 ctccgaccgc ttgctgccgg tggacttcgg cgaggtcgag atcggtcggc gcctctaccg
    12721 ctccggcgag aacgaatacc tgctcaaccg ccagaagatc cggctcaagg acctcgtcga
    12781 gctgctcgac gcgggcaacc tggccgacaa cgcgttcctg ttcatcggcc agggcatggt
    12841 cgaccaggcg ctcgcgctgc ggcccgaaga gcgccggccg ctgttcgagg aggcggccgg
    12901 tgtgcggcgc cacgagcgcc gccgccgcca ggcagaagcg gagctcctcg aagccgaggc
    12961 caacctggag cgcctgcgcg acctgctcgc ggagctgcgc ccacaggcca agcggctggc
    13021 ggcgcaggcg gagcagctgc aggcgcggcg ctccgcggga gcggagctgg ccgaggcatt
    13081 gctcgaagcc gcgcgagcgc ggtggggcgc gctctcgcag tcagcggcga aggaagcacg
    13141 ggaggtcgaa cgttcgcggg cagccgccga cgcggcgctg gccgagctga aggcagccga
    13201 gaccgaggcg acccggctcg cggccgagat ctcgcgccgc gcggaagcgg agcaggcgct
    13261 gcgccgcacg ctcgacaagc agcgcgagca actcacggcg ctccgcctgg accaggcgcg
    13321 gctcgagacg gaggtcgcgg cgcaggcgcg cgacgtcgag cgcgtcaccg ccgagcagac
    13381 ggccgccgag gcgcgccgcg cggccgcgct ggctgagctg agccgcccga tcccggaggc
    13441 cgatccagcg ctcgcgacgg agcttgacta cgttgagcgc cagctcgctg agctggagga
    13501 tgcgcatgcg gatgctgctg gcggcgagcc ggcgacggtc gacatcgccg cgcggcggcg
    13561 cgaggccgaa gggcacgagc gaggcctgac gacgctcgag aactcggtcg cgacggcgcg
    13621 tgcgcgcacg gctgaggtcg aggcgcgccg cctggctgcg gtccacgagc tggccgacgc
    13681 tgagcgtggc ctggagacgg cgagcaacga agagaaggct gccgccgagg acgtcgcggc
    13741 ggcacggatc gcggccgatg ccgcgctcgc ggcacgagcc ggagcgctcg cccgccttgc
    13801 tgaagcggaa gctgacgcgg gcgccgtccg cggacggctc gcggcactgg acgcggcact
    13861 ggccgcgctg agcgacgagg ggctggccaa ggctgcgcgg gcgcgcggcg gcgcgctcgt
    13921 ggcggaggga ctcgagatcg agccgcgttt ccgcgctgcg gtgtcggcgg cactgggcgc
    13981 ggcggcgagc ggcttcctgg tcgatgaggg gtcagtcacc gcactcgccg ggcgccgggg
    14041 caacctggcc gtttcgggcg tcgccgcgaa gccaacttcg ccggccgcgc ttgcgcagat
    14101 cacagaggcg gcgattgccg cgggtggcgg ccggctcagc gaggcgatca ggcgcgaccc
    14161 gcagtcacag gtgacgcgtc tcctggaacg tttggtctgg ctgcccgacc tcgcggcagc
    14221 gctgaacctg cgcgccgcgc tgccgcaggg ctggcgcgcg gtcaccctcg ccggcgaggt
    14281 ggtagccgac gacgggctga tccagctggc cgcgaccgag tcggtgctcg agcagcgggc
    14341 gcagcgcgat gcacaggcac agcagctgca ggaaatggaa gcgacgctcg acagcctgcg
    14401 cgcggaggtc aaggtcgcga ctgacggggc cgccaaggca gccgacgccc tggccgacgc
    14461 tggcaagcgg cacgaagcca cccgtcgcca gctgcgcagc gccgaggaga tcgagcgcct
    14521 cgcccagcgg cgcggcgagc agctcgtgcg cgaacaaacc tggcagaaat cgcagctcga
    14581 gcggctcgag tcggagacga ccgcgcagcg cgacctggtc ggtcgcatca cggcggacat
    14641 cgcccggctg gaagccgact ctggcacttc ggccgcgggg gccgcgccca ggtcaacggt
    14701 caagcgcgcc gccgagctgc gtgaacgcgc ggctgagctg cgccgtcaga tcgccagcca
    14761 ggccggggcc acgaacgccg cgcaggaggc gcgtcgccgg gcggaggtga cgctggcgat
    14821 ggacgaggcg cggctgcgcg acctcgcggc cgagcgccag cgcacggcgc tgcgtgacgc
    14881 ggagttgacg gaacgacgtg cccagctgat ggcgcagctc gagatcgcga cgaaggccga
    14941 cgctgaggcg gccgcgaacc ttgaggcggc gtacgccggc ggcgcggagg aacgcgcgaa
    15001 cctgaccgca gcagagactc gggccacgac cgcgcgcgag acgctgcgcg agcacgagac
    15061 ccgcagccgc gccgcggagg tccgagcgct ggagacgcgg ctgctcctcg aacagagccg
    15121 cgagggcctc ctcgtggaac tcgccgcgat tggtgatgac ggcctcgccg ccctgacgcg
    15181 ggccgcgggc gatgagccac cggccgaagg acacgaggcg gagccgggcg agctggagga
    15241 cctgctcagc cggacgctgg agcgctggtc ggccgacaag tcggcccccg agcctgtctc
    15301 gaccggcaag ctctcaagcc tgcgccgacg cttccacgag ctgggcgcag gaaacccatt
    15361 cgcggtcgag gagtacgccg agctgcgcga gcggctcgat gggctggagg cgcagcgcat
    15421 cgacgtcgag gccgcgatca gctcgacgcg cgacctgatc gcgtcgctgg gcgcgctcat
    15481 caacgaccag ttccgcgcca ccttcgccgc gctcgaagat gcctttgcgc ggcgcttcac
    15541 ggagctgttc ggcggcggcg aggcacagct ctcgctcacc gcgcccgacg atctctcgac
    15601 gaccggcatc gacatacacg cgcgtccgcc gggcaagaag cgccaaccat tgcagatgct
    15661 gtcgggtggg gagcgcgcgc tgaccgcggt gagcctgctg ctggcgatgc tcgaggtgcg
    15721 gcccgtgccg ttctgcgtgc tcgacgaggt cgatgccgcg ctcgatgaag ccaacgtggg
    15781 ccgtttctcg cgcgcgctgc gcggcctggc tgagcaaacg cagttcatcg tcatcaccca
    15841 caaccgcggc accatcgagg ccgcggacgc gctgtacggc gtgaccctgg gcgacgacgc
    15901 ggtcagtcgc atcgtgtcga tgaagctgcc ggctccgacc aatggcaagg agaacggcac
    15961 cgggaacggc aagcacgccg ccccggaact ggcggagacg acaccgactt gacgctcgaa
    16021 cacggcgtgg cgcgcaccag gtcaggcttc gtcagccggc tgcgcgaggc cctggggcgg
    16081 ccgctcgacg atgacacctg ggacgagatc gaggaggcgc tgatcgccgc cgatctgggc
    16141 gcggccctgg cggcgcgcgt cgtcgagggc gcccgggcac ggcgcgacct ggagccggag
    16201 atcgcggtca agaacgagct ggtcggcctc ttcgcgccgc gtcaaaccac gggctggccg
    16261 cgcattcccg gcggcggcac gccggcggtt gtgctggtcg tgggcgtcaa cggcactggc
    16321 aaaacgacga ccgtggcgaa gctcgggcag cgcttcaaga cgctcggcgc gaatgtgctg
    16381 cttgcggcgg ccgacacctt ccgcgcagcc gcgatcgaac agctcgaggt gtgggccaag
    16441 cgtgccgacc tgccgctcgt ggcccacgcc gccggcgcgg acccgtcggc ggtggtctac
    16501 gacgcgcttg atgccgcggc tgcccgcgcc agtgacgtgg tggtgatcga caccgccggg
    16561 cggctgcaca ccaagagcaa cctcatggag gagctggcca agatccggcg cacgatcggc
    16621 aagcggctcc cggacgcgca gcccgaggtg ctgttcgtcc tcgacgccac caccggccag
    16681 aacggtctct cccaggcgcg cgccttccac gaggcggcgg ggctgaccgg catctgtctc
    16741 acgaagctcg acaccacctc gcgcggcggc atcgcgttcg ccatcgagga cgcgctcgat
    16801 gtgccggttc tcttcgtggg cgtgggcgag aagatggatg acctgctcga gttcgcgccg
    16861 aacgacttcg tcgaggcgct cttcgcatag ccggcgcttg cgccgcgtcc aagagcttta
    16921 ggccacgggc tccatatctt cgatccggcg gatcttgcgg acgggcgaga accacagcgc
    16981 gagcacgccc agcaggccaa ctgtaggccc gatgaagagc atcgtccgca gacccagctg
    17041 cagcgccagg aaacccgcga ccagcgtcgc ggcgagctgc gcaaccagga tcgccacccg
    17101 aaccgtggca ttgacccggc ccagctgccg gtcgtgcacg atcgtctggc ggatggaggt
    17161 gtcggtgatg tcgaaggccg tcatcgccgg gtcggtgatg agctgctggc caatcaggaa
    17221 gagcatcgcc agcagtggcg caccgatggg cgcgagcggg atcgaatacg agcccagcat
    17281 ccccaccagc atggcgacga gcagcaccct gcccacgccg aagcggccgg tgagccgggt
    17341 cgtcagcagc gcgccgaaaa gcgagctgat gccgccgaca gcggccacga tgccgattgc
    17401 cgccgcgtcg agtcccaggt cctcgatcgc gaacaggtac cacagtgcgc cgaagacgcc
    17461 ccacatcgac gccgatgcca tcgatgccag ggtcagcgcg cgcaggaccg gctgcccggc
    17521 cacgagccgg aggcccagcg cgatctcgcg caggacgggc tcacgctccg accgatccgg
    17581 tggcggtgcc tcgcgcacgc ggatcgagcc gagcagtagc gcggagacga tgaagctgaa
    17641 cgcgtcgacc aggatggcca ccggcgcggt caggatctgc acgaggaacc cggccgagcc
    17701 gaaggccgca aactcggaca cggagcccga cgcggccagc gccccattgg cgcgcaccag
    17761 gtcggcgcgc gggacgatcg tcggcaggaa agcgttgtcg gcggtatcga agaatgtcgt
    17821 cagcaccgcc gtcaggaggg tcaccagcaa caagagaggc aaggacagcc aaccgccaaa
    17881 cgcggccagc gggatgaggc ccagcagccc ggcgcgtccc aggtcggccc agatcatcac
    17941 cgggcggcgg cgtagccgat caacccaagc gccggccacg agcccaacgg ttagcccggc
    18001 gatgagctcc atgctgcgca ccagtgccac gccgaccgag tcggctccca gggtcacgat
    18061 ggccacgaac ggcagggcga tccgcgtgat cagcgaaccg aagatcgaga tcgtcgccgc
    18121 cgaccacact cgcaagaagg cgttgttgcg ccacagcgcg ggcgtgggac gttcgtcctc
    18181 cggcgggaac ttgggatcga gcagcggctc agcggccatc gcgcgactgt acgggtacga
    18241 tcctttcagt gtttgacgat ctttccgagc gcctgcgcgg cgtcctatcg aagctcaccg
    18301 gccgcggccg catcacggag gccgacgttg acgcggccat gcgcgaggtc cgtctggcgc
    18361 tgctcgaggc cgacgtcaat ttccgcgtgg tcaaagattt cgtggcgcga gtgcgcgagc
    18421 gggccatcgg ccaggaggtg ctcgagagca tcaacgccgg ccagatggtc atcaagatcg
    18481 tccacgacga actgaccacc atcctgggtg ccggcgatcg gaccttccga ctggccggtt
    18541 cgcctgcagt cgtgatgctg gtcggcctgc agggctcggg caagacgacg accgccgcaa
    18601 agctggcgcg acagctcatc aagcagggcc ggcggccgct ccttgttgcc gccgatccct
    18661 accggccggc cgcggccgat cagctccaga cgctgggcgg gcagatcgac gtgccggtct
    18721 atcgcgcacc ggcggggacg aaggtgctcg acattggccg gggcgccgtg gcggaagcga
    18781 agaagtcggg cagggacacc gtcctgctcg acactgccgg gcgcacatcg ctcgatcagc
    18841 cgctgctcga cgagatcggc ggacttgcca aggcgctgga gccgtcagag accttgctcg
    18901 tcgtcgacgc gatggttggc caggagtcgg tcgcggtggc ccaggcgttc gccggagccg
    18961 ccccggtcac aggtctggtg ctgaccaaga tcgatggcga cgcccggggc ggcgccgcgc
    19021 tgtcgatcag cgccgtcacc ggcctcgcgg tcaagttcct gggcaccggc gagaagaccg
    19081 acgcgctcga gccgttccat cccgaccgcc tggccgggcg catcctgggc atgggcgacg
    19141 tgctgacgct cgtcgagaag gcgcaggagc acgtcgatca gaagcaagca gaagagctcg
    19201 cccgcaagct gcagaagaac acgttcacgc tcgaggattt cctgggccag atgcagcagc
    19261 tcaagaagat gggtccgatc ggcgggctga tcgacctgat cccgggcatg ggcggcgcgg
    19321 ccaaggaggc acaagcggcg gtcgagcgcg gcgatatgaa gcgcgtcgag gcgatcatcc
    19381 aatcgatgac cgtcgccgag cggcgcgacc cggacttgtt gagcggcagc cgcaagcgcc
    19441 gcgtggcgcg cgggtcgggc acgagcctgc aggacgtgaa ccggctgctc aagcagttcg
    19501 cggagatgaa gaagctgatg cgccagctgg gtggcgcggg gggccggcgt gggatgctgc
    19561 cgtttggccc gcgccgatag ggccatcggc ggttcgacaa accgggtcac ggatgggacc
    19621 atcgcactcc aatgagcgac aaagaaccgt catccttcaa cgacctgtcc ggatcacctg
    19681 acgagacccc cacgcgggtc gagttcaagc ctctgttcag ccgccccgcc agtgcgaccg
    19741 accagccagg ccccgtggag ccggcggcgg ccgtgacacc gactgcggca ccgtcctcga
    19801 tgaccatgaa cagccgctgg cgctgggccg tcgtgggcgt cgcgacgatt ttcgttgtcg
    19861 gcctgctggg cgctgtgttc atcctgtcgc agccgcgcgc gggcacgccg tcgactgtcg
    19921 cgcgctacgc gccggctgac acggcgctgt acatggaagc ccggctcgat ctgcccggcg
    19981 accaacacaa cctgattgcg cagttcatga gccatttccc gggtttcgcc gaccaggcgg
    20041 ccttcgacca gaagctcgat gagacattcg aatccatctt cgagtcgagc gacagcgggg
    20101 tttcctggcg ggacgacatc aagccctggt tcagcggcga gatcggcgtc ttcaccgcga
    20161 gggtcagccc ggctcccggc acgccaccgt cgatgacagt tgcgctgcgg gtcaaggagg
    20221 gccagaagag cgtgctcgac ggctggctga cgccgctcct ggggaccgac tggcagcagt
    20281 cgacgtacca ggatcaggcg atctggaccg gcaacttcgg caactcgctc gatcgcgtca
    20341 gtctcgccat gactgatgag gtgctactcg tgagcacgcg catcgaggac ctgcagaagg
    20401 cactcgacgt gggcgccgag cgcagcgccg gcatggccga tgacgccttc ttcctgcaac
    20461 agctggcagc gctccacgcc gaccggctgg ccacgttcta tttcgactac gcgtcgatgc
    20521 tcgaggggtt gccgtcgcag tcgctgttcg gctccggctg tttcgacagc gccacccagg
    20581 tggccaccca gatgaagttc gtgggcgagg tgcgcgcgga aagcgaccac atctccttca
    20641 ccgagcgcgt ccgggtgcca gcagtcgcag gcgctccgtc gcaggccggc tatgtcccgt
    20701 cccggctggc ggagctggcc tcggccgaca ccgtcctgtt ctacgaggtg ccccagctgg
    20761 gggcaaacat caagtatttc gtgggtgagc tcctgacatg cgccgaagcc agtgggggta
    20821 tgcctgacct gtcctcgatc gagacgatcc tgggcacgcc tctccctgac tacttcgact
    20881 tcgtccgcga cgctgcgctc gtcgtgaccg cagacggcgg cacgtacggc ggcgggctcg
    20941 tcgccagcgt cgatgacgag gcgatcgccc aatcacgcgt cgccaagctc atcgcctatg
    21001 tgcgactggc cgccgggagc atgggcgagt tcggcccgca gatcaccttc gaggagcagc
    21061 agcacggcaa cgccacaatc acggtgatca gcgtcagcga tcttccgcct gatgtcccgt
    21121 tcagctcgct cgccttcacc gtggccaatg ggcacctgat catcgggctg gacgacttcg
    21181 tcgcgaacac gctcgatcag acagtcgacg gctcactcgc tcgtacgcaa cgattcgcgg
    21241 ctggcgtcgc cgcgggcgga accaacaact atcccgcggc gttcgtcgac atcagcgcca
    21301 tccgggccag tttcgaggcg ctcatcccgg cgagcgagcg acctcgctac gacttggaga
    21361 tcaagccgta cgtagagcca ttcgactacc tgatgatcgt cggcgggagc cagggcgacg
    21421 tcggcatcag caacgtcttt ctctacgtga agtaacctgt tagccaatct gacagggagg
    21481 cttcacgcag atcaagtggc agtccgcatt cgtttgaacc gcgtcggcgc gaccaagcag
    21541 ccgacgtatc gcttcgtcgt gaccgagagc cgcaacgccc gtaacggccg gtcgctcgac
    21601 accatcggtc actacaaccc gcgtaccgac cccatcgaga tcaaggtcga tgaggacaag
    21661 gcgcgcgact ggatgaagcg cggcgcgctg ccgtcggcca cggtcgagcg gctcttccgc
    21721 cgcgtcggcg tgctgccggc caagggcgaa tagcggcatg tctgctgagt cgctggtcga
    21781 gtacgtcgcc aaggcgctcg tcgatgagcc ggacgcggtc gaggtcaagg ccgtcgacga
    21841 ccaggacggt cgcgtgatcg agctccacgt cgcagagacg gacatgggca aggtcattgg
    21901 ccgcaacggt tcggtcgcca aggcgatgcg cacgctgctc aaggtcgtgg ctgcgcgtga
    21961 aggggagtcg atctcgctcg agatcgtctg agtccggccg cgccgtgaat gacgaccgcc
    22021 tggcggtcgg cctggtgcgc ggcgtgcacg gcctgcgcgg cgcggtgcgg gtcgagatcc
    22081 tgagtgacaa cgcgagccgt ttcgaggtgg gcagcgtgct ctacccggag ggcggcgata
    22141 gaccactgac cgtcgcctca gcccaccgcg atgggccggg actgctggtc acctttcgcg
    22201 aggtgaagga ccgacccacc gccgacaccc tgcgcgagaa gtacctcgaa gccccgagcg
    22261 accgcacggc gctgccggcc gacacctggt actggcacga gatcgtcggc tgtgccgtcg
    22321 ccacggaatc cggcgccgag ctgggcacgg tgcgtgaagt cttccgggtc gctgaatcgg
    22381 aggtttacgt cgtggtcggc gaccgcggcg agatcctcgt gccagcagtg tcggcggtca
    22441 tcaccaagct ggatccgccc aacaggcaga tcgtcgtcaa gggcgcggcc ctggggatca
    22501 ccgaatgagc gcattgcgca tcgacgtagt gacgatcttc cccgagctgt ttcccggacc
    22561 gctcgcggcg agcatcccgg gtcgcgcgct cgagcgcggc ctggcgacgc tcaccgcgca
    22621 cgacctgcgc gagtggggcc tgggtcgcca ccgcagcgtg gacgactacc catacggcgg
    22681 cggcgtgggc atgatcatgc gccccgagcc catccatgcc gcgcttgccg cgctggcgga
    22741 tgagtcgacc acgaagatcc tgctcgatcc cggcggcgcg ccgttcaacc acgagttggc
    22801 gcgcgacctg gcggagcgtg agcacctgct cttcgtgtgc ccgcgctacg agggcgtcga
    22861 ccaccgcgtc cgctccatgg tcgatctcga gatctcgatc ggcgactacg tcgtgtccgg
    22921 gggcgagctg ccggccatgg tcgtgatcga cgcggtgctc cgcttgctgc cgggcgcgat
    22981 cgactccgaa tcgccaattg acgagtcttt cgcctccggc ggcctggaat atccgcaata
    23041 cacgcgcccg ccggagttcg aaggcatggg cgtgcccgac gtgcttgtca gcggccacca
    23101 cgcccagatc gagcagtgga agcgcgacca ggcccgctcg cgcactcgag agcagcgacc
    23161 cgacctactg gcggacgact agccgctcgc ccgactgctc gcagagcggc cctcgaagcc
    23221 cggccagccg gccgagtcga tcacgcagaa gcggttgcct tcggggtcgg ccatgatcac
    23281 gaagtcggca tcggccgcct tcttcggcca gtcgatctcg gtcgcgccca gactcttgag
    23341 gcgcttgacc tctgcggcct ggtcctcggc gtagagatcg agatggatgc gcggcgggat
    23401 ctggagttcg gagcgcatcg tgtcgagcga gacgttcggt cccgtgccgt cgcgcggccg
    23461 caggagtgcg aaagtgtcgt catcgtcgtc gcgcggcacg tagtccagca cgcgtgacca
    23521 gaactcgatc tggcgtggca gattcttgac gcggatgacg atggagccga ctaccaacac
    23581 gtcccagatc ctagcgccga tgtatactcc gcggcccgac caccagttgg cgcccgatat
    23641 tgcgcgcgcc ccatccagga ctgagagcca gcgtcaccat gagcgttctc gacgagataa
    23701 ccgcagagca cctgcgcacc gacctgcctg agctcagttc gggcgacacc gtcaaggtct
    23761 cggcgcgtgt gcgcgagggt aatcgcgagc gcatccagat cttcgagggc accgtcatgc
    23821 gcttgcgcgg cggtggtatc gcccgctcca tcaccgtccg cgccattcgc agcggcgtcg
    23881 gcgtggagcg caccttcaag gtccactcgc cgcgcatcga gaagatcgag gtcgtgcgcc
    23941 acggcgtcgc ccggcgcgcc cagctgtact tcctgcgcaa gcgcgtcggc aaggccgcgt
    24001 cactgcgaga gcgccggccc tcctagcgcc actccggccc gcggctcacg ccgcgacgga
    24061 ataatcttcg ccgcgatggg ggtcttcaat ccgacgcggc gcctcgagaa ccagctccag
    24121 cgcgagcatg gctaccggat catcctcggt ctcgatgagg ttggcgtcgc gccgtcgtgc
    24181 gggccggtgg tggcgtgcgc cctcgcgatg cccctggaca agcgtttcat cagcggcgtg
    24241 cgcgactcca agatgctgtc ggccgctcag cgcgagaagc ttgcccgcaa gatcctgcgc
    24301 caggcgctga ctgttggcct gggcgcggcg tcggtgcgcg agatcgatca actcaacatc
    24361 tactacgcca cgatcctggc gatgaagcgc gccgtaacgc gcgccggcgc gcacgacttc
    24421 gtcatcgtcg acggtcgcca cattcgcggc ttcgaggagc acgctggccc atacaaggcg
    24481 gtcatcgacg gcgacgcgcg cgtgtacgcg atctcgtgtg cgtcgatcat cgccaaggtc
    24541 acgcgcgacc ggctgatgca taagctcgcc gcgcgctacc cgggctatgg ctgggagcac
    24601 aacgcgggct acgccacgcc cgatcaccgc gccggcatgt cagcgctggg cgtgaccccg
    24661 caccaccgcc gttccttcat caccacgcag cgcgtccttg ctggcgaaca gacgtcgttc
    24721 gacctgctcg acgagctcgg cccacagacg ctcccagaac tcgagccgca aagcgatttg
    24781 gtgcctattt ccgcctaggc cgacgctaaa gtgcgccggt gaccatcgtt ctggggtacc
    24841 tcgccgccgc tgcggtcgcc acggtcggcg tgtggttggt ggcgggccgc aacttcgtga
    24901 gccagcgcga ccccgcggtc aacctcgccg atctgggcgc caagcaggtt ggccttatga
    24961 gcggcctggc gggctacgcc gtcacgggca tggtcttgct ggtgacgctg ggtcgcagca
    25021 tctcggacac atccagtacc gcctacacca cggtcgtcac gatgttcttc gtctcgtgga
    25081 tggcctacgc ggccaccgcg ttcctgttcg ccaacatgtc cgatcacgac gaagaggcac
    25141 ggcgccagcc gcgccacggg tttgacgctc cggccgtcca gtttgccggt gccgccggta
    25201 cgctcgagtt tgcctttggc cttggttggc ttgcgctgcg cccgctgttc ctctcgtttg
    25261 gcctgacgcg actggccgac ctggtagcgc tcatcctggt cggtgtcgcg ctcgcgagct
    25321 acggcctggt cgctcatcag ttgcatcgca gcggctacgg acccgcgcgc gtgttgaccg
    25381 cgatcccgct tctcaccatt gctgccacgc tggtctttgc gctcgtagtc ggcgcgctcg
    25441 ggctgcgctc cgagcagtct gcgctcgatc tgatcgtcgt cggcttcgcg cttggcgccg
    25501 tggcgttcgg gttcttcatc gccgtcaatg tcctgggcac ctacgggcca accgcccgat
    25561 tcctggcgcg gagcgccggc tacctcgtgc tggcgttcat ccaggccaac gtggtgctcg
    25621 tcgccttcct gctgctcgcg gtgctgggcc tcgcctgata agtcgccggt aagcggcgcg
    25681 cgtcaccctc aacggagtga agacggcgcg ccaacaagcc ggacgcgcgg ccgaagatga
    25741 ggtcgcggcc cacctcgccc tgagcggctg gcggctgctc ggacgcaaca tcgtggttgg
    25801 acgcgacgag atcgacatcc tcgccatcga ccccgggccg ccggcggagc tcgtgttcgt
    25861 ggaggtgcgc agcgccacga gcgatcgctt cggcaccccg gaggagcgcg tcgaccggga
    25921 caaggtcgcg cacctgtacc gtgcgagccg cgccctctcg gggccgtacg gcctgaggcg
    25981 ccgcgtggac ctcgtcgtcg tcgatcgccg cacaggccgg cgggcgctac gccacctgcg
    26041 ggcgctggaa cccgcctagc ggcccaggac gccgactcgt tgtagactgc gccagccgcc
    26101 caagcggcaa gcgccgtcag gcgccatcac acacgcgtgc tgctccccgc aaggtgcctc
    26161 acgggcctca cggccacgaa ggttgcggaa ccaggacaca cgcggaggca caaccgaacc
    26221 ctcaggaggt ccaactcccc gtggcaatgc ccaacgtgtc catgcgccag ttgctcgagg
    26281 ccggcgtgca cttcggtcac cagacccgcc gctggaatcc gaagatgaag cggtacatct
    26341 ttgccgagcg caacggcatc cacatcatcg atctcgccaa gacggtcaag ggcctcgagg
    26401 aggcgctcga gttcgtgcgc cagaccgtgg cccagggtga cacgatcctg ttcgtcggca
    26461 ccaagaagca ggcccaggag ccgatcgccg aggaggcgac ccgcgccgag atgccgtatg
    26521 tcaaccagcg ctggctgggc ggcatgctga ccaacttcgt gaccatccgc aagcgccttg
    26581 gcctactcga ccagctggag gcgcagcagc agaacggcga gttcgagcgg ctgacgaaga
    26641 aggaagcgag tgtcaagacc gagacgatga ccaagctcct gcgcaccttg ggcggcatcc
    26701 gcaagatgcg ccgcctgccg ggcgcgatgt tcgtcatcga tccacagcgt gagcacatcg
    26761 cggtgatcga ggcgcgcaag ttggagatcc cggtcatcgg caccggtgac accaacgtcg
    26821 atccggacat gctcgactac gtcattccgg ccaacgacga cgcgatccgc gccatccggc
    26881 tgctgtgctc gctcatttcg gacgcggccg tcgagggcgg ccacgagcgc gccgcgcgcc
    26941 cggtcgagga gccggtggca atcgtcgagg agcatgaaga ggcgagcgac gagatggtcg
    27001 ccgcgctcgc agctggtggc acgttcagct tcgagcccga gcccgacgag gaggagcttc
    27061 tcccggcgga ccgggcagtc gaggccgagg aggcggagga agaggcggag cctgaacccg
    27121 cgcgagccgc accggcgcgg cccgctgccc gagccggcgc cgcacgcact ggtgccgcgc
    27181 gacccggtgc tccgcgtacc ggcacccgtc gcccggcagc atcaaaggac aaggccaagc
    27241 ccgccgatga agaggatgcg ggctgagcct gagctcgtcg gatacccaag aagcgaacac
    27301 gaaggaaacc atggctgaca tctctgccca gaaagtaaag gagctgcgcg agcgcacggg
    27361 cgccggcatg atggagtgca agagcgcact cgtcgaagcc gacggcgacg tcgagaaggc
    27421 aattgccctc atccgcgagc gcggccaggc cacggccgcc aagaaggccg gccgcgatgc
    27481 ccgcgagggc gtcgtggcga gctacatcca cgccggtggt cggctgggag tgctcatcga
    27541 ggtcaacagc gagaccgact tcgtggcccg caacgacgag ttccagaagc tcgtcaagga
    27601 gctggccatg caggtcgccg ggctcgatcc caagtacccc acgatcgagt cgatccctgc
    27661 ggacgtgctc gaggccaaga aggccgagct cctggccggc gaagaggtcc agaagaagcc
    27721 cgagaacatc cgcccccaga tcatcgaggg ccagctcaag aagtggtatc agtcggtcgt
    27781 gctctacgag cagccgttcc gcgacaccga ccagaaggtg ggtgacctga tcaccgaggc
    27841 tatcgccaag atcggtgaga acattcgcgt acggcgcttc gtgcgctacc agctgggaga
    27901 ggacatttga gccaagccga tcgagattgg tcgtcgatcc ggtgacgagc tcagtggcgg
    27961 acgccccgag cgccgttcgc tacggccgga ttctgctcaa gatcagcggt gaggcgctgc
    28021 tcggccagcg cccctacggc gtagatcccg gtatgtgcac gttcatcgcc cagcaggtga
    28081 aggaagtgca cggccttggc gtccaggtgg cggtggtggt cggtggcggc aacatattcc
    28141 gtggaatggc cgccgcggcg agcggcatgg atcgcgcgac aggcgactac atcggcatgc
    28201 tcgccacgat catgaacggc ctggcgctcc aggatgccct cgagaaggct ggtgtgccca
    28261 cgcgcgtcat gagcgcgatc gccatgaacg aggtgtgcga gccgtacatt cgccgacggg
    28321 ccgtgcatca cttggagaag ggcctcgtga ccatcctcgt tgcagggacg ggcaacccgt
    28381 acttcacgac cgacacggcc gcggctctgc gcgcggtcga gatcggcgcc gaggtgctgc
    28441 tcaaggcgac caaggttgac ggcgtgtacg acagcgaccc cgtgaccaac ccggccgccg
    28501 tgcgccacga gcgcctgggc tacgagcaga tgctgaccga caagctgcag gtcatcgatg
    28561 ccacggccgt gacgctgtgc atggagaaca acctgccgat cgtcgtgttc gacatgaaca
    28621 agcccggcca catccgtgcc gcggccctgg gcggcagcat tggcacggtc atcgacaatg
    28681 gtgttggaca tgactgacga cattcgaaga gacgcagacg cgcgcatgag ccgggccatt
    28741 gaggccctgg agcgcgaaat ggccgcgatc cgtaccggcc gcgccgccac ggcgctggtt
    28801 gagcgcatcc aggtcgagta ctacggcacg cagacgccgc tcaaccagct cgcgagcatc
    28861 agcgtcccgg aggcgcacct catcgtcatc cagccgtggg accgcagcgc gctcggcgcg
    28921 atcgagaagg cgatcctcaa gagcgaggtc ggcctgacgc ccaacgtcga tggaaccgtc
    28981 gtgcgcctcg cgatcccggc gctgaccgag gagcgtcgca aggacctcgt aaagctcgtc
    29041 cacaagcgca tggaagaggc gaaggtcgag atccgcaacc accgccgcga cgtggccgac
    29101 gagatcaaga aggccgtgcg cgacggcgac atcggcgctg acgaagagcg gcgcgagatg
    29161 gagctgctgc agaaggtgac cgaccgctgg acgggtgagg tcgaccgcgt gggcaagcac
    29221 aaggaacagg agatcctcga ggtcggatga cctccgtgac cgccacggat c
//
LOCUS       DATBRX010000169         5297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_400032, whole genome shotgun sequence.
ACCESSION   DATBRX010000169 DATBRX010000000
VERSION     DATBRX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400032"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VIF08_06600"
     CDS             <1..612
                     /locus_tag="VIF08_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY8135695.1"
                     /translation="NSDACREHLSALWEAAATIGAAQIQNRGTIGGNAINASPAGDTL
                     PVLLATDAELVLGSARGERTVAAKDFWPAYRTTARAKDELLVRIRFPLPRGRQVRFRK
                     VGTRRAQAISKVVMAVSWREDGGVWRDVRVALGSVAATPMRATETERVLEGTAPRETT
                     ADHAAATLANEIHPIDDVRSTADYRRVVSARILHRLLREAGGW"
     gene            609..1205
                     /locus_tag="VIF08_06605"
     CDS             609..1205
                     /locus_tag="VIF08_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY8135696.1"
                     /translation="MKPENVRFFKSAADFREWLENYHDEAPFQWIGFWKKASGKPGMS
                     YDEAVLEALCFGWIDGQTNRVDDDAVTTRFSPRRAGSNWSQLNIARIKELRAAGRMHP
                     AGLAAFEARRDPEPGVYTYETRPADLPEDLSAIFRKNKGAWDFWRAQTPGYRKAMTWW
                     IVQGKQQQTRLRRLEALIAESAKGVKIGETSLPKLKAQ"
     gene            1202..2743
                     /gene="aceB"
                     /locus_tag="VIF08_06610"
     CDS             1202..2743
                     /gene="aceB"
                     /locus_tag="VIF08_06610"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY8135697.1"
                     /translation="MTESGRVRVVGASVPGIERVLTPDALQFLAGLANEFEPRRQEIL
                     AARRERATRLAVGARLDFLPETASVRDSDWRVAPAPADLNDRRVEITGPADPKMTINA
                     LNSGAKVFMADFEDSLSPTWANVVSGQAALQDAVRRTLRFDSPEGKHYELGENLATLV
                     VRPRGWHLVERHVEVGGQPISGSLFDFGLYFFHNAAEALGRGSGPYFYLPKMQSHREA
                     RLWNDVFTWSQAQLGIPHGSIRATVLIETIWAAFEMDEILYELRDHAAGLNAGRWDYI
                     FSIIKAFAATPDQALLPDRAQVTMAVPFMRAYQQLLVKTCHRRGAHAIGGMSAFIPNR
                     REPEVTEKALAKVREDKEREAGDGSDGTWVAHPDLVPVAMEVFDRALADRPNQKYRQR
                     EDVHTLADQLLDFHVPGGQVTDTGLATNVSVGVRYIDAWLGGTGAAALDNLMEDAATA
                     EISRSQVWQWVRTGRFSREQVLAELAPWRDRHPQAAALFEQVALSAEFVEFLTLPAYE
                     LLDQA"
     gene            complement(2818..3315)
                     /locus_tag="VIF08_06615"
     CDS             complement(2818..3315)
                     /locus_tag="VIF08_06615"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY8135698.1"
                     /translation="MRLLGRLTRLGPVALAVLLSLVLVAPVLATGPTNDRSHGRVLWA
                     RLAGAQEVPGPGDPDGRGGAKITLLPGYGTVCFRIRVRDITLPAAAAHIHAGERGVAG
                     PVVIALGAPDAEGKAKGCVTGVDPELIRAIWMNPRSYYVNVHTSDFPDGALRGQLHRN
                     NGRNS"
     gene            3539..4024
                     /locus_tag="VIF08_06620"
     CDS             3539..4024
                     /locus_tag="VIF08_06620"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY8135699.1"
                     /translation="MSAPTIAVERPVLTSAPRRFVLYRHRSVAHAELGETISMTFGEL
                     YACVEACGANPGGPPFVIYQSGSEPGVRWEIDICAPVAVPMAAPLCADYIELPGGSVV
                     SLLHVGPYDTLGESYGQIAAYIGEHGLQQAGPPREFYYSEPDVPPAETRTLIEWPVNQ
                     P"
     gene            complement(4038..>5297)
                     /locus_tag="VIF08_06625"
     CDS             complement(4038..>5297)
                     /locus_tag="VIF08_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8135700.1"
                     /translation="REVDASGLLVLPGVVDVHTHTRVATDELPDRFFQDSVAAAFGGT
                     TTFLAFNNPGTGSEQTGSLPTDAQAWRRQTDSDSAVDYGLNLVLQPAHADELAAEIPA
                     VVDMGVPTFKAFMVYDFGLSPAAISLALRAAARSRGMLMVHGEDRTALEANIARLKAQ
                     GKTQPRYHAESRPPYVEAAGTRMAVEMARAADAPLYLVHLSSQEALAEVKGGRAVGAQ
                     VFVETCPHFLVLDESRYELPDVDCACFIISPPLRSAADRDALWAALADGAINVISTDH
                     VPDTVAEKRGWDECFDLISNGAPGIETLLGNVYGQGVARGRLTIERMVDVLSTTPARL
                     FGMPSKGALEPGKDADIVLFDPQERRTVTQAELHHTSDFTPYEGLATSGIVRSTIVRG
                     EFVIRDGHFVGRRGYGQFVERVLSADG"
BASE COUNT          855 a         1800 c         1845 g          797 t
ORIGIN      
        1 aactccgatg cctgccgcga acatctgtcg gcgctctggg aggccgcggc gaccatcggc
       61 gcggcgcaga tccagaaccg gggcacgatc ggcggcaacg ccatcaacgc atcacccgcg
      121 ggcgacacgc tgccggtgct ccttgcgacg gacgcggagc tggtgctcgg cagcgcgcgc
      181 ggcgagcgga cggtcgccgc gaaggacttc tggccggcgt atcgcacgac cgcgcgagcc
      241 aaggacgagc tgctggtgcg catccgattc ccgctgccgc gcggtcgcca ggtgcgcttt
      301 cgcaaggtcg gcacgcggcg cgcgcaggcg atcagcaaag tggtcatggc cgtgagctgg
      361 cgcgaggacg gcggggtgtg gcgcgatgtg cgggtggcgc ttggctccgt cgccgcgacg
      421 cccatgcgcg cgaccgagac cgagcgggtg ctcgagggca cggcgccgcg cgaaacaacc
      481 gccgaccacg cggccgcgac gttggcgaac gagatccacc ccatcgacga cgtgcgctca
      541 acggccgatt accggcgggt cgtctccgcg cgcatcctgc accgcctgct gcgcgaggcc
      601 ggcggctggt gaagccggag aacgtccgct tcttcaagtc ggcggccgat ttccgtgagt
      661 ggctcgagaa ctaccacgac gaggcgccat ttcaatggat cggcttctgg aagaaggcgt
      721 caggcaagcc cggcatgagt tacgacgagg ccgtgctgga ggcgctgtgc tttggctgga
      781 tcgacggcca aacgaaccgc gtcgacgatg acgcggtcac cacgcgtttc tcaccgcgcc
      841 gagctggcag caactggagc cagctcaaca tcgcgcgcat caaggagttg agggctgccg
      901 gtcggatgca tccggcgggt ctcgccgcat tcgaggcgcg acgcgatccg gagccaggcg
      961 tctacaccta cgagacgcgc ccagctgacc tgccggagga cctgtcagcc atttttcgca
     1021 agaacaaggg ggcgtgggac ttctggcgag cacagacgcc gggttaccgt aaggccatga
     1081 cgtggtggat cgtccagggc aagcagcagc agacgcggct gcgccgactc gaggcgctca
     1141 tagccgagtc cgccaaaggc gtgaagatcg gcgaaaccag cctgccgaag ctgaaagccc
     1201 aatgactgag tcagggcggg tgcgggtagt cggtgcatcc gtgccgggca tcgagcgcgt
     1261 cctcacgccc gacgcgctgc aatttctggc cggcctcgcg aacgagttcg agccgcgccg
     1321 ccaggagatc ctcgccgcgc ggcgcgagag agccacacga ctcgccgtcg gagcgcggct
     1381 cgacttcctg cccgagaccg ccagcgtccg cgacagcgac tggcgcgtcg caccggcgcc
     1441 cgctgacctg aacgaccgtc gcgtggagat caccggccct gccgatccca agatgacgat
     1501 caacgcgctc aactccggcg ccaaggtctt catggccgat ttcgaggact ccctgtctcc
     1561 aacgtgggcc aacgtggtca gcggccaggc cgcgctccag gacgccgtgc gcaggacgct
     1621 gcgcttcgat tcaccggagg gcaagcacta cgagctgggc gagaacctgg cgacgctcgt
     1681 cgtcaggccg cgcggctggc acctggtgga gcgccacgtc gaggttggcg gccaacccat
     1741 ctccggcagc ctcttcgact tcgggctgta tttcttccac aacgcggcgg aggcgctggg
     1801 gcgtgggtcg gggccgtact tctacctgcc caagatgcaa tcgcaccgcg aggcccggct
     1861 ctggaacgac gtcttcacct ggtcgcaggc ccagctcggc atcccgcacg gatccatccg
     1921 cgccaccgtc ctcatcgaga cgatctgggc ggctttcgag atggacgaga tcctgtacga
     1981 gctgcgcgac cacgccgccg gcctcaacgc cggccgctgg gactacatct tcagcatcat
     2041 caaggcgttc gccgcgacac cggaccaggc cctgctgccg gatcgggcgc aggtcacgat
     2101 ggccgtgccg ttcatgcgcg cctatcagca gctcctcgtg aagacgtgcc atcgccgggg
     2161 agcccacgct atcggcggca tgagcgcgtt cattcccaac cggcgcgagc cggaggtgac
     2221 cgagaaggcg ctcgccaagg tccgcgagga caaggagcgc gaggcgggtg acggctccga
     2281 tgggacctgg gtggcccatc ccgacctcgt accggtggcc atggaggtgt tcgaccgggc
     2341 cttggccgac cggcccaacc agaaataccg ccagcgcgag gacgtgcaca cgctggccga
     2401 ccagctactc gatttccacg tccccggcgg gcaggtgacc gacaccggcc tggcgacgaa
     2461 cgtgtcggtc ggtgtgcgct acatcgacgc ctggctgggt ggcacgggcg cggcggcgct
     2521 cgacaacctg atggaggacg ccgcgacggc ggagatctcg cgctcgcagg tgtggcaatg
     2581 ggtgcgcacg ggacgcttca gccgcgaaca ggtcctggcc gaactcgccc cgtggcgcga
     2641 tcgtcacccg caggcagccg cgctcttcga acaggtggcg ctgtccgccg agttcgtcga
     2701 gttcctgacg ctgcccgcct acgagctgct cgaccaggcc tgagcgccaa tagaaaagcc
     2761 cgcggccggg gggaccgacc gcgggcgagg gtggagggcc gacgaaggcc tcggacgtta
     2821 gctgttgcga ccgttattac ggtgcaactg gcctcgtagg gccccatcgg ggaagtcgga
     2881 ggtgtgcacg ttgacgtagt agctgcgcgg gttcatccag atcgctctga tcagctctgg
     2941 atcaacgccg gtcacgcagc ccttcgcctt cccctcggca tctggtgcgc cgagcgcgat
     3001 caccacggga ccggcgactc ctcgctctcc ggcgtggata tgcgcggctg cggctggcag
     3061 ggtgatatcc ctgacgcgaa tccggaagca gaccgtgccg tagccgggca gcagcgtgat
     3121 cttggcgcct cccctgccat cgggatcgcc cggacccggg acttcctggg cacccgccag
     3181 gcgtgcccat aggacgcggc cgtgggaccg atcattggtc ggcccggtgg ccaggactgg
     3241 tgcgacgagc accaggctca acaggacggc cagggcgacg gggcccaagc gcgtcaaacg
     3301 gccaagcaaa cgcatggctc ctcccattct tgggcgcgtt ggaacgcgca tgcgcgaccg
     3361 gacacggctg accgggcgtc gcacgtccac cctaaaccga tgggtcgcgg tttagcaaac
     3421 cctgagcgtt cgctgagccg gaaaggctga cggcttactg actttcggca ctcgcggctg
     3481 cggtccgggt ggccgactat cgccgcggtt gccctgtccg tcatccggag gtgtgcccat
     3541 gtccgcgccg accatcgccg tcgagcggcc agtcctgaca tcggccccgc gtcgatttgt
     3601 gctctatcgc caccggtcgg tggctcacgc cgagttgggc gagacaatca gcatgacctt
     3661 cggcgagctg tacgcgtgcg tcgaggcatg cggcgccaac ccaggcggcc cgcctttcgt
     3721 gatctaccag tcgggctcgg agcccggggt gcgctgggag atcgacatct gcgcaccggt
     3781 cgcggtaccc atggccgcgc cgctctgcgc ggattacatc gagctgcccg gcggcagcgt
     3841 cgtctcgctg cttcacgtgg ggccgtacga cacgctgggc gagtcgtatg ggcagatcgc
     3901 ggcgtacatc ggcgagcacg gcctgcagca ggccggaccg ccgcgcgagt tctactacag
     3961 cgaacccgac gtgccgccgg ccgagacgcg cacgctgatc gagtggccgg tcaaccagcc
     4021 ttagcgctca gtcagcttta gccgtcggcg ctcagcacgc gttcgacgaa ctggccgtag
     4081 ccgcggcggc cgacgaagtg cccgtcgcgg atcacgaact cgccgcgcac gatcgttgag
     4141 cgcacgatcc cgctggtggc gaggccctca tagggcgtga agtcgctggt gtgatgcagc
     4201 tcggcctggg tgacggtgcg tcgctcctgc gggtcgaaca gcacgatgtc ggcgtccttg
     4261 ccgggctcga gcgcgccctt gctgggcatg ccgaacaatc gcgcgggcgt cgtcgacagc
     4321 acgtcgacca tccgctcgat ggtcagccgg ccgcgcgcca cgccctggcc gtagacgttg
     4381 ccgagcagcg tctcgatgcc aggcgcgccg ttgctgatca ggtcgaagca ctcgtcccag
     4441 ccgcgcttct cggcgaccgt gtctggcacg tgatcggtcg agatgacgtt gatcgcgccg
     4501 tcagccaacg cggcccaaag ggcgtccctg tccgcggctg agcgcagcgg cggcgagatg
     4561 atgaagcagg cgcagtccac gtcgggcagc tcgtagcgcg actcatcgag caccaggaag
     4621 tgcgggcacg tttcgacgaa gacttgggca ccgaccgccc gccccccctt gacttccgcc
     4681 agggcctcct gcgacgaaag gtgcacgagg tacagcggcg cgtccgcggc gcgcgccatc
     4741 tccactgcca tgcgcgtgcc cgcggcctcg acgtagggcg gccgcgactc cgcgtggtag
     4801 cgcggctgcg tcttgccctg ggccttgagg cgcgcaatgt tggcctccag ggcggtgcgg
     4861 tcctcgccgt ggaccatgag catgccgcgc gaccgcgccg ccgcccgcaa cgcaaggctg
     4921 atcgccgccg gcgaaaggcc gaagtcatag accatgaatg ccttgaaggt cgggacgccc
     4981 atatcgacca cggccgggat ctcggccgcg agctcgtcgg cgtgggcggg ttggagcacc
     5041 aggttgagcc cgtagtcgac ggccgagtcg gaatcggtct gcctgcgcca cgcctgcgca
     5101 tcggtcggca gcgagcctgt ctgctcggag cccgtgccgg ggttgttgaa cgccaggaac
     5161 gtggtcgtgc cgccgaacgc ggctgccacg gaatcctgga agaagcggtc gggcagctca
     5221 tcggtcgcga cgcgggtgtg ggtgtggacg tcgacaacgc cgggtaacac caggaggccg
     5281 cttgcgtcga cctcgcg
//
LOCUS       DATBRX010000170         4118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_176474, whole genome shotgun sequence.
ACCESSION   DATBRX010000170 DATBRX010000000
VERSION     DATBRX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176474"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..978)
                     /locus_tag="VIF08_06630"
     CDS             complement(166..978)
                     /locus_tag="VIF08_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8135701.1"
                     /translation="MRIALVQAGTFTTDAGAAFGPVPRTMWQRLVEAELNPNSTLNQA
                     LNCLLIEAPGGRVLVETGIGERMDEHHREQRGVTGLPILPALRAAGFEPDTVDLVALS
                     HLHFDHAGGLLTSEGGQAFPRARVVAQADEWEFALGTNPRLIASYEQPDLRLVEPWGR
                     GGSAQGDEEVLPGVSVVRTGGHSGGHQAVVVRGPNRTVGFFGDLCMRPWSANPRWVPS
                     FDDFPLTSVEVKASLFRQAAEEGWTVVLSHEPRQPVGRFVADRDRFRFEPTL"
     gene            complement(1017..1622)
                     /gene="dcd"
                     /locus_tag="VIF08_06635"
     CDS             complement(1017..1622)
                     /gene="dcd"
                     /locus_tag="VIF08_06635"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY8135702.1"
                     /translation="MSVLSDRDIRAALQSGRVRIDPYDPSCLQPSSVDLHLDAEFRVF
                     RNNRYPFIDVRAPQPDLTELIKVGDDEPFILHPGEFVLGQTLEWIELPDDMVVRLDGK
                     SSLGRLGLLIHSTAGYVDPGWKGNLTLELSNVANLPIALYRGMKIGQISFFNMSSPVE
                     RPYGSRELGSKYQGQSTPTASQFYKDFDADRIKVKKGGPKR"
     gene            1652..1960
                     /locus_tag="VIF08_06640"
     CDS             1652..1960
                     /locus_tag="VIF08_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135703.1"
                     /translation="MTDLLSKRRIAVRGTLIALAVSAVIGVGVVAAVWLVTREVNETS
                     LRAAAYLALGYLTLNLIVLGIGLLARPSDAWALGAAFATPLVIAAIGFGYTIFLAKPL
                     "
     gene            complement(1957..2475)
                     /gene="nrdR"
                     /locus_tag="VIF08_06645"
     CDS             complement(1957..2475)
                     /gene="nrdR"
                     /locus_tag="VIF08_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY8135704.1"
                     /translation="MRCPNCGERDTRVVDSREIDDAAIRRRRECNLCATRFTTYERID
                     AARLTVLKSDGKREEFDREKLASGLAKALTRRPVPEDAAQKAAEEIETALRARGTSEV
                     PSRLIGEAAMAKLRELDQIAYIRFASVYESYEGLEDLRKEVDTLLAEREVEPEARGGG
                     GGSAAGKRYPKR"
     gene            3212..4063
                     /locus_tag="VIF08_06650"
     CDS             3212..4063
                     /locus_tag="VIF08_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135705.1"
                     /translation="MAGQAAIAVDPSDGSVWIAFVYWRCTDCAPAGSDGIFIVNSVGG
                     TWSAPVQRESTGAVSPSLAVRAGRVYLAWELPGDYGHRYGPVVFGADVSGAWQTQPLV
                     QRGEQPSLVLKADGRVGVLFAGANAMRYADQKPNGSFVIEPLPGTSDASGTLWDSALA
                     FDAVTGETWAAWTGGTSGVHPVFVAKRGPDGWSDPIAALPDGDLSGLGVRAGVVQLTA
                     EKSTGGLIFASNGSGAFVEQMVDAARTYWDDAAFALLPSGRPVIVIARDTPGNDSGMW
                     FLKGPPA"
BASE COUNT          670 a         1376 c         1356 g          716 t
ORIGIN      
        1 cacgccgctg ctgctcggcg ttctcgcggt cagcgtgatc ctggtgttcg cgatcgtgct
       61 cgacggcgag gccaacgcgc tggaaggcgc gatgctcctt gggctgtacg tgatcgtggc
      121 ggccgccgtc tggtgggggc cgcagatcca cggctgagcg gtgatctaaa gggtgggttc
      181 gaacctgaac cggtcgcgat cggcgacgaa gcgccccacg ggctggcgcg gctcatgcga
      241 cagcacaacg gtccagccct cctccgccgc ctggcggaac agcgacgcct tgacctcgac
      301 gctcgtcagc gggaagtcgt cgaacgatgg cacccagcgc gggttggcgc tccacgggcg
      361 catgcacagg tcgccgaaga agccgactgt ccgattggga ccgcggacga cgaccgcctg
      421 gtgaccgccc gaatgaccgc cggtgcggac caccgacact cccggcagga cctcctcgtc
      481 cccctgggct gatccgccac ggccccacgg ctcgaccagg cgcaggtcgg gctgctcgta
      541 agaagcgatc aatcgggggt tcgtgcccag ggcgaactcc cactcgtccg cctgcgcgac
      601 gacgcgtgcc cgcgggaatg cctgcccacc ctctgacgtc agcaggccac cggcgtgatc
      661 gaagtgaagg tggctgagcg ccacgaggtc gaccgtgtcg ggctcgaagc cggcggctcg
      721 gagagcaggg agaatcggca atcccgtcac cccacgctgc tcgcgatggt gctcgtccat
      781 ccgctcgccg attcccgtct cgaccagcac gcgcccgccc ggggcctcga tgagcaggca
      841 gttgagtgcc tggttcaggg tcgagttcgg gttcagctct gcctcgacca ggcgctgcca
      901 catcgtccgc ggcaccgggc caaatgcggc gcccgcatct gtcgtgaagg tgccggcctg
      961 gacgagcgcg atgcgcactc cgccgcccag ctcggcgcgc cagtcgaccg tctcgttcag
     1021 cgtttggggc cgcccttctt gaccttgatt cgatcagcgt caaagtcctt gtagaactgg
     1081 gacgcggtcg gtgtcgactg gccctggtat ttcgagccga gctcccttga tccatatggg
     1141 cgctcgaccg ggctcgacat gttgaagaag gagatctggc cgatcttcat gccgcggtac
     1201 agggcgatcg gcaggttggc gacgttggag agctcgagtg tgaggttgcc cttccagccg
     1261 ggatcgacat agccggcggt cgagtggatc agcaggccca gccggccaag gctcgacttc
     1321 ccgtccaggc gcacgaccat gtcatccggc agctcaatcc actcgagcgt ctgacccagg
     1381 acgaattcgc ccgggtggag gatgaatggc tcgtcgtccc caaccttgat cagctcggtc
     1441 agatcgggct ggggcgcgcg gacgtcaatg aacgggtagc ggttgttgcg gaacacacgg
     1501 aattccgcgt cgagatgcag gtccaccgac gacggctgga ggcagctcgg atcgtacggg
     1561 tcgatgcgga cacggcccga ctggagggca gcgcgaatgt cgcggtctga caggactgac
     1621 atcggccgga ttatctagga tcgactcgac gatgaccgac ttgctgagca aacgccgcat
     1681 cgccgtccgc ggcaccctca tcgcgctggc cgtctcggcc gttatcggcg tcggcgttgt
     1741 cgccgccgtg tggctggtta cccgcgaggt caatgaaacc tcgctgcgcg cagccgccta
     1801 cctggccctg ggatatctga ccttgaattt gatcgtgctg ggcatcgggc tgcttgcgcg
     1861 accctcagac gcctgggcac tcggcgccgc gttcgcgacg ccactggtca ttgcagcgat
     1921 tggcttcggc tacacgattt tcttggcgaa gccgctctag cgcttggggt agcgcttccc
     1981 ggccgcgctg ccaccgccgc caccgcgcgc ctcgggctcg acctcgcgct cagcgagcaa
     2041 cgtgtcgacc tccttgcgca ggtcttcgag gccctcgtac gactcgtaga cgctggcaaa
     2101 gcggatgtaa gcgatctggt cgagctcgcg cagcttggcc atcgccgcct cgccgatgag
     2161 ccgcgacggc acctccgacg tgccgcgcgc gcgcagcgcc gtctcgatct cctcggctgc
     2221 cttctgggcc gcgtcctcag ggacaggccg ccgggtcagg gctttggcga gccctgacgc
     2281 gagcttctcg cggtcgaact cctcgcgctt gccgtcgctc ttgagcacgg tcaggcgcgc
     2341 ggcatcaatg cgctcgtagg tcgtgaagcg cgtggcgcac aggttgcact cgcggcgacg
     2401 gcggattgcg gcatcgtcga tctcgcgcga atcgacgacg cgcgtatctc tctccccgca
     2461 gttagggcaa cgcacaggcg gcaatacctc gctggttagc cactacaggt agtggtttga
     2521 ggagactgta ccactagata gcgggcacat ttctgggcat cgagaaaatc gggacatccg
     2581 acacttgact ctcggcggcc acgtctacga cacgtagggc ccatacgtag cgcgtcctgt
     2641 actccgacgg tgtcatctga cgctcgagag gcatctactg gtcatggagg taggtcgcat
     2701 gtcgaaactg acaccgagtc ggcatatgca cagtcacacg atggccgccg cgctgatcgt
     2761 gctcgcactg gcggcctgta catctgctcc gaccatcaca cccacccctg cgccaacggc
     2821 gacggcgatc cccactctcc tgccgacccc acagccgacg cccacaccct ctgcgatccc
     2881 aacgccgagc ccgaccacgt cagcaacccc tatggccacg ccgacggcca cgcccactgc
     2941 gactccgaca gcgactccga cagcgactcc gacagcgact ccgacagcga ctccgacagc
     3001 gacgcctacg ccggtcgcgt ggccagccgc caaacacgtc gcccgtggcg ggttctcgca
     3061 gcctgccgtt gcagtcgatg cagctggcag ggttcacatc gcagcggctg ctgccgacaa
     3121 cgacgggatc tggtacctga cgaacgcgtc gggtgactgg gtcgcgcagc aggtggtggc
     3181 gccgtggtat tccgatcttg aggagatcgg catggcaggc caggcggcga tcgccgtcga
     3241 tcccagcgac gggtcggttt ggatcgcctt cgtctattgg cgttgcaccg actgcgcgcc
     3301 agccgggagc gacggcatct tcatcgtcaa cagcgtcggc ggcacatgga gcgcgcccgt
     3361 ccagcgagag agcaccggag cggttagtcc gtcactcgcg gtgcgcgctg gtcgggtgta
     3421 cctcgcgtgg gagctaccgg gagactacgg ccaccgttac ggaccagtcg tgtttggcgc
     3481 ggacgtctcc ggcgcctggc agacacagcc attggttcag cgtggcgagc agccgagcct
     3541 cgttctcaaa gcggatggcc gcgtaggggt ccttttcgcc ggtgccaatg caatgcgcta
     3601 cgccgatcag aagccgaacg gcagcttcgt gatcgaaccc ctgcccggca cctcggacgc
     3661 gtccggaaca ttgtgggact cagctcttgc attcgatgcc gtcactggtg agacctgggc
     3721 cgcgtggacc ggcggcacct ctggtgttca ccctgtcttc gtcgcaaagc ggggaccgga
     3781 cggctggtcg gaccccatag ccgctctgcc ggacggtgat ctgagcggac ttggcgtacg
     3841 tgcgggggtg gtgcagctga cggcagagaa gtcgactggc ggcctcatct tcgccagcaa
     3901 cggcagtggt gcattcgtgg aacagatggt cgacgcagcg cgaacatatt gggacgatgc
     3961 agcgtttgct ctgctgccgt cgggacggcc ggtgatcgtc atcgcgcgcg acactccggg
     4021 caacgactcc ggcatgtggt tcctgaaagg accgcccgcc taactcgtgg cgcggatcag
     4081 gatgatcggc gtcagctcct cgtcctggcc catggggt
//
LOCUS       DATBRX010000171         3715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1853851, whole genome shotgun sequence.
ACCESSION   DATBRX010000171 DATBRX010000000
VERSION     DATBRX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853851"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /gene="uppS"
                     /locus_tag="VIF08_06655"
     CDS             <1..372
                     /gene="uppS"
                     /locus_tag="VIF08_06655"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY8135706.1"
                     /translation="QTRASVADALSATEAGHRMTLNVAFNYSGRSEIVDAVRRAVTDG
                     LMPADIDEEAIRARLYTADGPELDLLIRTGGEQRISNFLIWQAAYAELYFTDVLWPDF
                     APATLDAALAEFTRRSRRFGR"
     gene            584..1450
                     /locus_tag="VIF08_06660"
     CDS             584..1450
                     /locus_tag="VIF08_06660"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8135707.1"
                     /translation="MRTRLISAAVLVPVVVILFVIGDVWLALGIAVLSAFVAYEAAQL
                     FRRAGLPASTWLAVIWTPLAVFWVGASVEASFELRHLVIAPGIAILVIAAAVVAVAQA
                     DPAIGLRIWIGTVMAALYAGMLGFMVLFVGIGPDLADSALFGYPMNSGKIWLLILVAT
                     VWTLDSAAYVVGRFYPRGHFMNHISPKKTWSGAIGGTIAAVAVCALLATGAGMAALGG
                     VVLGLIIAVAAQLGDLVESMLKRAAGVKDSGSLIPGHGGFLDRVDSFLIAAPALYTAL
                     FVCAGLSATSWL"
     gene            1447..2604
                     /gene="dxr"
                     /locus_tag="VIF08_06665"
     CDS             1447..2604
                     /gene="dxr"
                     /locus_tag="VIF08_06665"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY8135708.1"
                     /translation="MSDRVRVAVLGSTGSIGRQALDVLEAHSDRFVVIALAAGRDEAA
                     FAEQVARHKPAYTALAKDGPGALAEIAAIPDVDLVLVGTPGIVSLVPTLAALERGKLV
                     ATANKETLVSGGHLVMPLARDLAARHGFRDAATGALSWLRPIDSEHSAIWQCLTGERR
                     EDVARLILTASGGPFRDWSADRLRSATPADALAHPTWRMGAKVTIDSATLMNKALEVI
                     EARWLYDFGDDQVDVVVHPQSVIHSMVEFVDGSIKAQLGLPDMRLPIQYAITYPERLP
                     SPAPRLAAAGIPTLGFEPVDAKRFPALDIGRQAGQQGPRASAALIAADDIAVERFLSG
                     SLSFTGIADVCADAVVRFGDGAAPDLPELLALDAEVRAWAATATPAGASRP"
     gene            2604..>3715
                     /locus_tag="VIF08_06670"
     CDS             2604..>3715
                     /locus_tag="VIF08_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEY8135709.1"
                     /translation="MFDFIGLGNGLINVVLLLGFLVVLVVIHELGHFIVARRSGVRVH
                     EFGIGFPPRARVLGTDSKGTMYTLNWLPIGGFVKLEGEEGESVEDDAFVNKPLRTRLA
                     ILLAGVLMNFLLAFVVFSFIAGVSDPVADIRIGQVDAASPAASVGLVGSKLLSVNPDG
                     TKNYDDSGDLIIAIDGQRFPVFDHIDDAAQALTYLRAHAGQQVTLTVRNAAGEVRDVA
                     VTLRQPGPDQGALGIRDLRFVPSNITHDPLTAMSLGARRTIDAATIVLRGLGQLVTNI
                     GNPPVSGPVGMVDIIGQVRSIGQPIWLLWLFGVLSANLAVVNVLPFPPLDGGRVAVAL
                     IQRATGNRISVAAERLAYLTGFVLLMALLVWVTFFD"
BASE COUNT          520 a         1271 c         1231 g          693 t
ORIGIN      
        1 cagacacgcg cctctgtggc cgacgcgctc tcggcgaccg aggccggtca ccgcatgacc
       61 ttgaacgtgg cgttcaacta ctcgggccgg tcagagatcg ttgacgccgt gcgccgcgcc
      121 gtcaccgacg gcctgatgcc agcagacatt gacgaggagg ccatccgcgc ccgcctctac
      181 acggccgatg gaccggagct ggacctgctc atccgcaccg ggggcgagca gcggatcagc
      241 aacttcctga tctggcaggc agcctacgcg gagctgtact tcaccgacgt gctgtggccc
      301 gacttcgcgc cggcgaccct ggacgcagcg ctggccgagt tcacccgccg cagccgccgc
      361 ttcggccgct agccgggcgg atgggaccga tctggccggc gcctctgccg tcccacgttc
      421 gaatacaccg ctttccgcct tgcccgggcc tgattttcga tcaatcctgc cgttccacgg
      481 ttggattcac cgaacttgag ccagattccg ggctgcgcgc gtcccgctcc ggtgcattca
      541 accgccccac gtcaggtcat ctggtgcagg tagccttcgg gatatgcgca cccgtctgat
      601 cagcgcggcc gtgctcgtgc cggtcgtcgt gatcctgttc gtgatcggtg acgtctggct
      661 ggcgctgggc attgccgtcc tgtcggcgtt tgtcgcatac gaggccgcgc agctgttccg
      721 tcgtgccggc ctgcctgcat cgacctggct cgccgtgatc tggactccgc tggcggtgtt
      781 ctgggtcggc gcctcggtcg aagccagctt tgagctccgt cacctcgtta tcgcgccggg
      841 catcgcgatc ctggtgatcg ccgccgcggt cgtagccgtg gcgcaggcag acccggcgat
      901 cggcctgcgc atctggatcg gcacggtgat ggcagccctg tacgcgggca tgctcggctt
      961 catggtcctc ttcgtgggca tcgggccgga cttagcggac agcgcactgt tcggctaccc
     1021 gatgaacagc ggcaagatct ggctcctaat cctggtagcc acggtgtgga cgctcgacag
     1081 cgcggcttac gttgtcggac gcttctatcc gcgcggccat ttcatgaacc acatctcacc
     1141 gaagaaaaca tggagcggag ccattggagg gacgattgcc gcggtcgctg tgtgtgccct
     1201 cctggcgacc ggagcgggta tggctgcgct tggcggcgtg gtcctgggac tgatcattgc
     1261 cgtggccgcg cagttgggtg acctcgtcga gtcgatgctc aagagagcgg cgggcgtgaa
     1321 agattccggg agcttgatcc cgggccatgg cgggttcctg gatcgggtcg actcgttcct
     1381 gatcgcggcg cctgcgcttt acacggctct cttcgtgtgt gccgggttat cggcgacctc
     1441 gtggctgtga gcgacagggt ccgagtcgcg gtgctaggga gcacgggctc gatcgggcgg
     1501 caggcgctcg acgtcctcga ggcgcacagc gaccgcttcg ttgtgattgc cctggccgcg
     1561 ggccgcgacg aggccgcgtt cgccgaacag gtcgctcgcc acaagccggc ctacaccgct
     1621 ctggccaagg acggaccggg cgcgctggcc gagattgccg cgatccccga tgtcgatctc
     1681 gtgctcgttg gcacgccggg catcgtgagc ctcgtgccga ccctcgccgc gctcgagcgt
     1741 ggcaagctgg tggcgacggc aaacaaggag acgctcgtca gtggcgggca cctggtgatg
     1801 ccgctggcgc gcgacctggc ggcgaggcac ggcttccggg acgcggcgac cggcgcgctc
     1861 agttggctgc gccccataga cagcgagcac tccgcgatct ggcaatgcct caccggggag
     1921 cggcgcgagg acgttgcgcg cctgatcctg accgcgtccg gcgggccgtt ccgcgattgg
     1981 tcggccgacc gcctgcgatc ggcaaccccg gccgacgcgt tggcccaccc cacgtggcgc
     2041 atgggcgcga aggtcaccat cgactcggcg acactcatga acaaggcgct cgaagtgatc
     2101 gaagcgcgct ggctctacga tttcggcgac gatcaggttg acgtcgtcgt ccatccgcag
     2161 agcgtcatcc attcgatggt cgagttcgta gacggctcaa tcaaggctca gctgggactg
     2221 cccgacatgc gtctccccat ccagtacgcc atcacctacc cggagcgtct gccgtcgcct
     2281 gcgccgcgcc ttgcggccgc gggcataccg accctgggct tcgaacccgt cgatgccaag
     2341 cgcttcccag ccctggacat cggccgtcag gcgggccagc agggtccgcg cgcgtcagcc
     2401 gcgctcatcg ctgccgacga catcgccgtc gagcgctttc tgtccggtag cctgagcttc
     2461 accggcatcg ccgatgtgtg tgccgacgcg gtggtgcgtt tcggcgatgg agcggcgcct
     2521 gacctgcccg aattgctcgc gctcgacgcg gaagtccgtg cctgggccgc aactgccaca
     2581 cccgctggag cgtctagacc ctgatgttcg acttcatagg cctgggtaac ggcctgatca
     2641 acgtcgtcct gctgctcggc ttcctcgtcg tgctggtagt catccacgag ctgggccact
     2701 tcatcgtcgc gcgccgctct ggcgtgcgtg tccacgagtt cggtattggc ttcccgccgc
     2761 gcgccagggt cctcggcacg gatagcaagg gcacgatgta taccctcaac tggctgccca
     2821 ttggcggctt cgtcaagctc gaaggcgagg agggggagtc ggtcgaggac gacgccttcg
     2881 tcaacaagcc gctccggacg cgcctggcga tcctgctcgc cggcgtcctc atgaacttcc
     2941 tcctcgcgtt tgtcgtgttc tcgttcatcg ccggtgtctc tgaccccgtg gccgacatcc
     3001 gcatcggcca ggtggacgcc gcgtcgccgg cggcgtcggt cggcctcgtc ggcagcaagc
     3061 tcctgagcgt caatcccgac ggcacgaaga actacgacga cagcggcgac ctcatcatcg
     3121 ccatcgacgg ccagcggttc ccggtgttcg accacatcga tgatgccgca caggcgctca
     3181 cctatttgcg cgcccacgcc ggtcagcagg tgacgctcac ggtgcgcaac gccgcaggtg
     3241 aggtgcgcga tgtcgcggtg accctgcgcc agcccgggcc cgaccagggc gcgctgggca
     3301 ttcgcgatct gcgcttcgtt ccgagcaaca tcacccacga tccgcttacc gcgatgtcgc
     3361 tcggcgcgcg gcggacgatt gacgccgcga cgatcgtgct gcgcggcctg ggccagctcg
     3421 tgaccaacat cggcaacccg cccgtctctg gcccggtcgg gatggtcgac atcatcggcc
     3481 aggtccgttc gatcggccag cccatctggt tgctgtggct ctttggcgtg ctgtcggcca
     3541 acctggctgt cgtgaacgtg ctgccgttcc cgccgctcga tggcggtcgc gtcgcggtcg
     3601 cgctcatcca gcgcgcgacg ggcaaccgga tcagcgtcgc cgccgagcgg ctcgcctatc
     3661 tgacgggctt tgtgctgttg atggcgctgc ttgtctgggt gacgttcttc gacat
//
LOCUS       DATBRX010000172         7903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2971071, whole genome shotgun sequence.
ACCESSION   DATBRX010000172 DATBRX010000000
VERSION     DATBRX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2971071"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..693
                     /locus_tag="VIF08_06675"
     CDS             289..693
                     /locus_tag="VIF08_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135710.1"
                     /translation="MDSTSRRPVGTLARALQGRPEPPAPPRGPISRVNNSLGLRRPAP
                     PPVNGAGMPRLPFGLPNLRRNVCPHYYMTGPRGPIPITAPHLLKALMRQESNVARCQL
                     RGGVHLEQGYVSDICLSPRFNQCVFYEDELNP"
     gene            725..1978
                     /locus_tag="VIF08_06680"
     CDS             725..1978
                     /locus_tag="VIF08_06680"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY8135711.1"
                     /translation="MASESDPAYQAVLARLAARGRFGIRLGVGRTRALLKRMDDPQLG
                     LPGVLIGGTNGKGSTQAMVASVLRAGGLRVGQTPKPHLLSYRERIVVDGSIIGVDDFV
                     DLITEVLDAADAVTVRHGPPTEFEVITAAAFTWFRRSGVEVGVIEVGLGGRLDATNAW
                     QGGVTAITNVALDHMEYLGDTVEAIAKEKAAIIKRGDAAAVTGATEPALSVIRGRARR
                     VKVPLEVRTPEDFDPDLKVGLLGRHQVANASVAAGVIDALSAAGIARVSDDAVRRGFA
                     DVTWPGRLELIRGKAGRPDILFDGAHNPAGITALAEALGELRQQLGDRPPALLMGVLA
                     NHWQPGMLDPLKQALPGSLLIATTVPATSNSLPPADLSAAWGKNSAAIADTDAAFEVA
                     LAGARGTLIVCGSLYLVGYVRNKVT"
     gene            1978..2883
                     /gene="folP"
                     /locus_tag="VIF08_06685"
     CDS             1978..2883
                     /gene="folP"
                     /locus_tag="VIF08_06685"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8135712.1"
                     /translation="MDATPSVSLPPDAESGVPQRPPQALEIGGRLFAWGTRTYVMGII
                     NVTPDSFSGDGLLAHTDPVRAAVAQARQMAAEGADLLDIGGQSTRPGHAQISAEEELT
                     RAVPVVAAVRAALPAMPLSIDTTSGRVAAAALDAGADLINDVWGTAAEPGLLSLAAER
                     GTPVVLMHNRAEARYANVVAEVIADLQHGLDAAVRAGISWERLIVDPGIGFGKTPEHN
                     LAVLHDLVALRVLARPILLGASRKSTIGKVLDVPADQRVEGTLATTALGVAAGIDIVR
                     VHDVQPNVRAARMADSVIRPGWGHS"
     gene            2884..3249
                     /gene="folB"
                     /locus_tag="VIF08_06690"
     CDS             2884..3249
                     /gene="folB"
                     /locus_tag="VIF08_06690"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY8135713.1"
                     /translation="MSDHIYLANMTFEVHIGAGDGERADEQPIEIDVDMTVDLKPAGD
                     SDDLEKTVDYGAVFRLSQQVAEGRPYRLLEALAERLAGEILAVESAVQNVVVTVRKPG
                     VPIDGVLDYAGVRVERSRA"
     gene            complement(3258..4592)
                     /locus_tag="VIF08_06695"
     CDS             complement(3258..4592)
                     /locus_tag="VIF08_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8135714.1"
                     /translation="MSDPKDDAPDASTQPQTPYSYSPPTEPRSQWAAPGWAHGSPPHW
                     FEPLPGQERRRPRRGRSGTYMVALFIVALLGSVLGSALTYVALLGSGQLRGGEVNVAV
                     ASPTPLPAASVTPRPHTGDDISTITAAAEQVSPAVVTITVEAAAATDPLSLPETGIGS
                     GIIYDTAGWILTNRHVVAEAAKVTVDLNDGREVEGTVYGVDTLTDLAIVKITATDLHA
                     APIGDSSQLQPGETAIVIGSALGTFTNSVTSGVISALGRSLTVTDPLNGERRRLRNLI
                     QTDAAINPGNSGGPLVDSSGTVVGVSTAYAEGAQGIFFAIPINIAKPIMAQAVAGEPL
                     TRPWIGIIYVAVDRALAAENNLPIDYGAWLSPDTGSGESPVKEGSPAAEAGLQPGDIL
                     TSIDGARIDASQSLDDILSNHDPGDVLPIEVLRAGETLQLTVTLGVRPAGLE"
     gene            complement(4632..6185)
                     /locus_tag="VIF08_06700"
     CDS             complement(4632..6185)
                     /locus_tag="VIF08_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8135715.1"
                     /translation="MPQLLPRFLTGLRARLVLGFLLVVAIVLALMFATLPRLLDGYFA
                     QQAQEDLGRRSGEIRQLVISRLLLYQSAGVEAPRPILQPTDPLTVAPGLLDSFGTSDE
                     GYVFESARDIAQANVSITIAPDQEHATDIVYRLDVPLPDQYAQPGQQRESIQGDPYTF
                     TFSDLYWTQAGAAAPQRLVTVTLSDPFTYRAQTIETIIGVMGVAAVLALIAAVILSLL
                     IANRLSKPIRRLTGAARELSEGHLDTRVPSPGNSPEMNDLTTAFNAMAERVQDSIEFI
                     RRDRDRSRDFLADVSHELRTPIAALRTFNELLTEGQVPEEATRQEFLEQSRQQIERLD
                     WLASNLLELSKLDSGLVLLDLRPDDLRAVVENAVQQAQPSADRKGVTLAVHLPDEPLR
                     QRHDPQRMGQVLGNLLGNAIKFTPAGGRIDISLEPTDGGMEFRVTDTGVGIDARELPY
                     VFDRFYRGTQAIEPRATGSGLGLSIVRSIVEMHNGRVAVTSTPGVGTQVSVTLPADVS
                     VSSPAPARA"
     gene            complement(6199..6915)
                     /locus_tag="VIF08_06705"
     CDS             complement(6199..6915)
                     /locus_tag="VIF08_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8135716.1"
                     /translation="MAATILIVEDEYAVARGTQYALQQEGYDVTVARSGEEGLEIATR
                     AAPDLIVLDVRLPEMDGFEMLRRLRGAGNKAPVLFLTARDEEMDKVIGLELGADDYLT
                     KPFGLRELMSRIKSLLRRAYGDLADAAGGRVIRHHDLLIDLERRRVQRGERRLSLTAT
                     EFEILRHLASRPGRVFSRRELIELVRDYEALDQDEKTINVHVSHLREKLEDDPSDPQF
                     ILTIRGAGYAFAERGSGAER"
     gene            6942..7610
                     /locus_tag="VIF08_06710"
     CDS             6942..7610
                     /locus_tag="VIF08_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY8135717.1"
                     /translation="MSRPQLSARPRDVRGKAVAHLRKGGALPAVVYGAGVQSQNISLD
                     THEFELLRRRTGRNAVIDLSIEGDGKAQPVLLQAIQEHPVSRHPLHVDLLVVNLEEER
                     TVDVPLVFVGTSEAVAKQGGVLLHLRDSVLVRCKPDDLPSGIEVDISPLVDFESALHA
                     SDLVMPAGVTLMTDASEAIARVQQPRIEEEPVAAPAAATEEGAEPKAEGAAEGGETAA
                     EETA"
     gene            complement(7711..>7903)
                     /locus_tag="VIF08_06715"
     CDS             complement(7711..>7903)
                     /locus_tag="VIF08_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135718.1"
                     /translation="AVHVWSVRADGPEATSVNSRQVVASSAMGVMVGVGTTGVVGPDV
                     GVAGPGEDGPGVGDGAPTV"
BASE COUNT         1220 a         2701 c         2718 g         1264 t
ORIGIN      
        1 gcgcgcggca gcatggcgac gcggtttgct ggcctggcgc gtccgacgcg ggcggtgggc
       61 acctgcgcgc ctgtcgctcg gaccgccgcg agctttcgca ttgcggggtc gaggcattgg
      121 cgcgattgtg gcagggctgg aggcatcgcg ttcgacggat gcaccgcctc gcgtcggcgg
      181 tcgcgttgcg cgcgggccgg tacttgcgcc gtgtcaggag cggtacgaaa tccggtgcgc
      241 gcaggcacgt ccgagcgccc ggtggtgcgt gtacgctacg gcggctctat ggactcgacc
      301 tcgcgccggc ctgtcggaac gctcgcgcgt gcccttcagg gccgccccga gccgcccgcc
      361 ccgccgcgcg gtcccatcag ccgcgtcaac aacagcctgg gactgcgccg tcccgcgccg
      421 ccgccggtca acggcgccgg catgccgcgc ctgcccttcg gtctgcccaa cctgcgccgc
      481 aacgtgtgcc cgcactacta catgaccggg ccgcgcggtc ccatcccgat caccgcaccg
      541 cacctgctca aggcgctgat gcgccaggag tccaacgtcg cacgctgcca gctgcgcggc
      601 ggcgtccacc tcgagcaggg ctacgtatcg gacatctgcc tatccccgcg tttcaaccag
      661 tgcgtcttct acgaggacga gctcaaccct tagccccggt tgccgctgcc ctaggctttc
      721 gctcgtggcc tcggaatccg atccggcgta ccaagccgtc ctcgcgcgcc tcgccgcccg
      781 cggacgcttc ggcatccgac tcggcgttgg ccggacacgc gcactgctca agcgaatgga
      841 cgacccgcag cttggcctgc ccggcgtcct gatcggtggc accaacggca agggctcgac
      901 ccaggcaatg gttgcctccg tgttgcgggc gggtggcctc cgcgtcgggc agacgcccaa
      961 gccgcacctt ctttcgtatc gcgaacggat cgttgtcgac ggctcgatca tcggggtcga
     1021 tgacttcgtc gacctgatca ccgaggtcct ggatgctgcc gatgccgtga ccgtcaggca
     1081 cgggccgccg accgagttcg aggtcatcac cgcggccgca ttcacctggt tccggcgcag
     1141 cggcgtcgag gtgggggtca tcgaggttgg gctgggtggc cggctcgatg ccacgaacgc
     1201 atggcagggt ggtgtgaccg cgatcacgaa tgtcgcgctc gaccacatgg agtacctggg
     1261 cgacacggtc gaggcgatcg ccaaagagaa ggccgcgatc atcaagcgcg gcgatgcggc
     1321 agcggtcaca ggcgcgactg agcctgcact gagcgtcatc cgcggccgcg cccgccgcgt
     1381 caaggtcccg cttgaagtgc gtacgcccga ggactttgat ccagatctga aggtcggcct
     1441 gttggggcgc caccaggtcg ccaacgcatc ggtcgccgcg ggcgtcatcg atgccctcag
     1501 cgccgccggc atcgcccgcg tatcggacga tgcggtcaga cgaggtttcg ccgacgtgac
     1561 ctggcccggc cgtctcgagc tgatccgtgg caaggccggg cgaccggaca tcctgttcga
     1621 tggcgcccac aacccggctg gcatcaccgc tttggccgag gcgctcggcg agctgcgcca
     1681 gcagctgggt gaccgtccac cggccctcct gatgggcgtc ctggccaatc actggcaacc
     1741 gggcatgctc gatcctctca agcaagccct gcctggctcg ctgctgatcg cgaccacggt
     1801 tccggcaaca tcgaactcgc ttccgcccgc ggatctctca gccgcctggg ggaagaactc
     1861 ggccgccatc gccgacacgg acgccgcgtt tgaagtggca ctggccggag ctcgcggcac
     1921 gttgatcgtt tgcgggtcgc tgtaccttgt cggttacgtg cgcaacaagg tcacttgatg
     1981 gacgcaacac cttcggtcag cctgccaccg gacgctgaat cgggcgtccc gcagcgcccg
     2041 ccacaggcgc tcgagatcgg tggccggctc ttcgcctggg gcaccaggac gtacgtgatg
     2101 ggcatcatca acgtgacgcc cgactcgttc agtggcgacg ggctgctcgc gcacacggat
     2161 ccggttaggg ccgccgtcgc gcaggcgcgg cagatggcgg cggagggggc cgacctgctc
     2221 gatatcggcg gccaatcgac gcggcccggt cacgcgcaga tttctgcgga agaggagctg
     2281 actcgcgcag tccccgttgt cgcggccgtg cgcgcagcgc tgcccgccat gccgctcagc
     2341 attgacacga cgtcgggccg cgtggcggcg gccgcactgg atgccggcgc cgacctgata
     2401 aatgacgtgt ggggcaccgc ggcggagcca gggctgctca gcctcgccgc cgagcggggg
     2461 acgcccgtcg tccttatgca caaccgggcc gaggcgcgct acgcaaacgt ggtcgcggaa
     2521 gtcatcgctg atctccagca cggactcgac gcggctgtgc gtgccggcat ctcctgggag
     2581 cggctgatcg tcgatcccgg catcggattc ggcaagacac ccgaacacaa cctcgccgtg
     2641 ctgcacgacc tcgtcgcgct cagggttctg gcaaggccca ttctgctcgg cgcaagtcgc
     2701 aaatcaacaa tcggcaaggt gctcgatgtt ccggcagacc agcgcgtcga gggcaccttg
     2761 gccacgaccg cgctgggagt ggcagccggg attgatatcg tccgggtcca tgacgtccaa
     2821 cccaacgtgc gcgcagcgcg catggccgat tcagtcatcc gcccgggctg gggccattca
     2881 taaatgagcg atcacatcta tctggccaac atgacattcg aggtgcacat cggcgccggt
     2941 gacggcgagc gcgcggatga gcagccgatc gaaatcgatg tcgatatgac cgtcgatctc
     3001 aagccggcag gcgattcgga cgacctcgaa aagaccgtcg actacggcgc ggtcttccgg
     3061 ctgagtcagc aggtcgccga gggtcggccg taccggctgc tcgaggccct ggcagagcgc
     3121 ctggcggggg agatcctggc cgtcgaatcg gccgttcaga acgtggtcgt caccgtgcgc
     3181 aagcccgggg tgccgatcga cggcgtgctc gactacgccg gggtgagggt cgagcgctca
     3241 agggcttaac tccggcgcta ttcgagtccg gctggcctaa ctcccagggt gacggtgagc
     3301 tggagcgtct cgccggcgcg cagcacttcg atcggcagca cgtcgcccgg atcgtggttg
     3361 ctgaggatgt cgtcgaggct ctgcgaggcg tctatccggg cgccgtcgat ggaggtgagg
     3421 atgtcgcccg gctgcaaacc ggcctctgcc gcggggctgc cttccttgac cggtgactcg
     3481 ccgctgccgg tgtccggtga aagccacgcg ccatagtcga tgggcaggtt gttctccgcg
     3541 gcgagagcgc gatcgaccgc gacgtagatg atcccgatcc acggcctggt gagcggctcg
     3601 ccggctacag cttgggccat gatcggcttg gcaatattga tggggatcgc gaagaagatg
     3661 ccttgcgccc cctcggcgta ggcggttgat accccgacca ccgtgccgct gctatcgacg
     3721 agcggtccgc ccgagttgcc ggggttgatg gcggcatctg tctggatcag gttgcgcagc
     3781 cggcgtcgct caccgttgag cggatcagtc acggtcaagc tccggcccag cgccgagata
     3841 acgcccgagg tcacgctgtt ggtgaaggtg cccagggcgc tgccgatgac gatggcggtt
     3901 tcgccgggct ggagctgaga cgagtcgccg atgggcgcgg cgtgcaggtc ggtcgcggtg
     3961 atcttgacga tcgccagatc ggtcagcgtg tccacaccgt agaccgtgcc ctctacctcc
     4021 cggccgtcgt tcagatctac ggttaccttg gccgcttccg caacgacgtg acgattggtc
     4081 aggatccagc ccgcggtgtc gtagatgatg cccgagccga tgccggtttc cggcagcgag
     4141 agcggatcag tcgccgcggc cgcctcgacg gtgatcgtca ccacggctgg gctgacctgt
     4201 tccgcggccg cggtgatcgt ggagatgtcg tcgcccgtat gcggtcgcgg cgtgacggac
     4261 gccgcgggta atggtgtcgg gctcgcgacg gcaacgttga cctcgccccc acgcagctgg
     4321 cctgagccga gcagcgcgac ataggtcagc gcggacccga ggacgctgcc cagcagggcg
     4381 acgatgaaga gggcaaccat gtacgtgcct gagcgcccgc gacgcggccg gcggcgctcc
     4441 tgtccgggga gcggctcgaa ccagtgcggc gggctgccat gcgcccaacc aggcgcggcc
     4501 cactggctgc gcggctctgt gggtgggctg taggagtacg gcgtctgggg ctgcgtcgac
     4561 gcgtcgggcg cgtcgtcctt gggatcgctc aagctctgct cacggccggc ctagagtgcc
     4621 acgaacccgg ttcaggcgcg ggcaggggcc ggtgaagaaa cggacacgtc cgccgggagc
     4681 gtgaccgaaa cctgggtccc gacgcccggc gtgctggtga ccgcgacgcg gccgttgtgc
     4741 atctccacga tcgagcgcac gatcgacagc cccaggccgg agcccgtggc gcgcggctcg
     4801 atcgcctgcg tgccgcggta gaagcgatcg aagacatacg gcagctcgcg cgcgtcgatg
     4861 cccacgccgg tgtcggtgac gcggaactcc ataccgccgt cggtcggctc gaggctgatg
     4921 tcgatcctgc cgccggcggg cgtgaacttg atcgcgttgc ccagcaggtt gcccagcacc
     4981 tggcccatgc gctgcgggtc atgacgctgg cggagtggct cgtcgggcag atggaccgcc
     5041 agcgtcacgc ccttgcgatc ggccgatggc tgagcctgct ggaccgcgtt ctcgaccacg
     5101 gcgcgcaggt cgtccgggcg caggtcgagc agcaccaggc cggagtcaag ctttgacagc
     5161 tcgagcaggt tgctcgcgag ccagtcgagg cgctcgatct gctggcggct ctgctccagg
     5221 aactcctggc gcgtcgcttc ctcgggcacc tggccttcag tgagcagctc gttgaaggtg
     5281 cgcagcgcgg caatgggcgt gcgcagctcg tgcgacacgt cggccaggaa gtcgcgcgag
     5341 cggtcgcggt cacgcctgat gaactcgatc gaatcctgga cgcgctcggc catggcattg
     5401 aacgcggtcg tcaggtcgtt catctccggt gagttgcccg gtgacggcac gcgcgtgtcg
     5461 aggtggccct cactcagctc cctggcggca ccggtgaggc gtctgatggg tttcgaaaga
     5521 cggttcgcaa tgagcagcga caggatgacc gccgcgatca gggcgagcac ggccgcaacg
     5581 cccatgacgc cgatgatcgt ctcgatggtc tgcgcccggt aggtgaacgg gtcggacagc
     5641 gtgaccgtga ccaggcgctg cggagcggcc gcgccggcct gtgtccagta caggtcggag
     5701 aacgtgaacg tgtacggatc gccctggatg gattcgcgct gctgaccggg ctgcgcgtat
     5761 tggtcgggga gcggcacgtc caggcggtag acgatgtcag tcgcatgttc ctggtcgggc
     5821 gcaatggtga tgctcacgtt ggcctgggcg atgtcgcgcg ccgactcgaa gacgtagccc
     5881 tcgtcggagg tgccgaatga gtcgagcagt cctggggcga cggtcagcgg gtcggtcggc
     5941 tggaggatgg gtcgtggcgc ctcgacaccg gccgactggt atagcagcag ccgactgatc
     6001 acgagctggc ggatctcacc ggatcgccgg cccaggtcct cctgcgcctg ttgagcgaaa
     6061 tagccgtcga gcaggcgtgg cagcgtcgcg aacatcaacg cgagcacgat cgctaccacc
     6121 aacaggaagc ccagcacgag gcgggcccgc agcccggtca ggaagcgcgg cagcagttgt
     6181 ggcatgagtg gccccgggtt aacgctccgc gccagatccg cgctcggcga aggcatagcc
     6241 ggccccgcgg atcgtcagga tgaactgcgg atcggaggga tcgtcctcca gcttctcgcg
     6301 caggtggctg acgtggacgt tgatcgtctt ctcgtcctgg tcgagcgcct cgtagtccct
     6361 gacgagctcg atcagctcac gccgcgagaa gacgcgtccg gggcgcgacg cgaggtggcg
     6421 caggatctcg aactccgtcg cagtcagtga aagccggcgc tcgccgcgct ggacgcgccg
     6481 ccgctcgagg tcgatcagca gatcgtgatg gcggatcacc cgtccgccgg ccgcgtcggc
     6541 caggtcgcca taggcgcggc gcagcaatga cttgatgcgg ctcatcagct cgcgcagacc
     6601 gaatggcttc gtcaggtagt cgtcggcgcc cagctcgagg ccgatgacct tgtccatctc
     6661 ctcgtcgcgc gccgtgagga agagcaccgg cgccttgttg cccgcgcccc gcagccggcg
     6721 cagcatctcg aatccgtcca tctccggcag ccgcacgtcg agcacgatca ggtcgggcgc
     6781 cgcgcgcgtc gcgatctcca ggccctcttc gccgctgcgc gccacggtta cgtcgtaacc
     6841 ctcctgctgg agggcgtact gcgtgccgcg cgcgaccgcg tactcgtctt cgactatcag
     6901 aatcgtggcc gccacgcctc gcatcgtaca atcagccgtc catgagccga ccacagcttt
     6961 ccgcccgccc gcgcgacgtg cgcggcaagg ccgtcgccca cctgcgcaaa ggcggcgccc
     7021 tgccggccgt cgtctacggc gccggcgttc agtcacagaa catctcgctc gacacccacg
     7081 agttcgagct gctccgccgc cggaccgggc gcaacgccgt catcgacctc tcgatcgagg
     7141 gcgatggcaa ggcccagccc gtgctgctcc aggccatcca ggagcacccg gtcagccgcc
     7201 acccgctgca tgtcgacctg ctcgtcgtca acctcgagga agagcgcacg gtcgacgtgc
     7261 cgctcgtctt cgtgggcacc tcggaggccg tcgccaagca gggcggcgtg ctgctccacc
     7321 tgcgtgactc ggtcctcgtg cgctgcaagc cggacgacct gccgtcgggc atcgaggtcg
     7381 acatctcgcc gctggtcgat ttcgaatcgg cgctccatgc atccgacctg gtcatgcccg
     7441 ccggcgtgac cctgatgacc gacgcctcag aagcgatcgc gcgcgtccag cagccgcgca
     7501 tcgaagagga gcccgtcgct gctcccgccg cggctaccga ggagggcgcc gagcccaagg
     7561 ccgagggcgc agcagagggt ggcgaaaccg cagccgagga gacggcgtaa ccccggccat
     7621 gaaaccgggg ctgctcgcca gccttatctg cgccgccctc gccgcgtgcg gcacgggcgc
     7681 accagcaccg accaatacgc aggcaccgac tcaaacggtt ggtgccccgt cgcccacgcc
     7741 cgggccgtct tcgcccgggc cagccacgcc cacgtccgga cctacaaccc cggtcgtgcc
     7801 aacacctacc atcacaccca tcgcggagga cgcaaccacc tggcgggagt tgaccgaggt
     7861 cgcttccggt ccgtcagccc gcactgacca cacgtggaca gct
//
LOCUS       DATBRX010000173         7250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1630137, whole genome shotgun sequence.
ACCESSION   DATBRX010000173 DATBRX010000000
VERSION     DATBRX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630137"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /gene="hydA"
                     /locus_tag="VIF08_06720"
     CDS             complement(<1..1195)
                     /gene="hydA"
                     /locus_tag="VIF08_06720"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY8135719.1"
                     /translation="MKSLITNGTVVNADGSAVADVLVDGEQVMAVGRGLAGSALAQGV
                     EQTIDATGKYVIPGAIDVHTHMELPFGGTFAKDTFESGTRAAAFGGTTTIIDFAVQSQ
                     GKSLREGLDAWHAKAQGNATTDYGFHMIMSDVNDATLAEMDQLVAEGVTDFKLFTAYP
                     GVFYSDDGAIFRAMQQTAKNGGLIMMHAENGLAIDVVAAQAVADGKTDPYYHGVVRYP
                     IFEGEATNRVIRLAEAAGVPVYIVHLSARDALQMVREARDRGAMAFAETCPQYLFLSL
                     DDMANGFNGAKFVCSPPLRPADHQVDLWHGLVKDDLQVVSTDHCPFDFETQKQLGVGD
                     FRKVPNGLPGVEDRVDLLHDGGVVGGRITRERWVEIVSAAPARMFGLAGVKGVVAPGA
                     DADLVI"
     gene            complement(1220..1951)
                     /locus_tag="VIF08_06725"
     CDS             complement(1220..1951)
                     /locus_tag="VIF08_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135720.1"
                     /translation="MRLLSEGVYYRNRDAASSVGVPQLLLGLAVLILAGLVLGGGAIW
                     LLGNSRPGATPTNIAVVPTATPLASGPTPSAAFSPTVPPTPTLIATGSPSFLPTFTPT
                     PSPAPTPTPVPTPAPTPVDCALASQGTAVKQFQLGLGHPTAKPLPKTWCIRHVTIDQW
                     VQWGTAKLFNKNQLVYQATCLPGSCPTGSMDFVPPYQANQGRTLRYEFSCYEDPLTPD
                     PFFECSDATQEGAVITIDYEAFAAP"
     gene            complement(2060..2710)
                     /locus_tag="VIF08_06730"
     CDS             complement(2060..2710)
                     /locus_tag="VIF08_06730"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY8135721.1"
                     /translation="MVEPRGGGGSGAVVLAVALLAGFLFIGGAAAIYLSNPPGPADPT
                     DIALASGSPTLPPFVQPTATPSPSPTFILLPSESPSFSALPSSTAIGPTAPPTPIRPT
                     NPPTAAAVVVKFTCAQLGAQRKITCSDNSSGPIESRQWDFGDGSPPVTERNPTHEYPS
                     SGPNEYTVTLVVTGTDGQQYFKQKKVVFQAPPTPTPAPTETPEPTATPTPVGSFAS"
     gene            complement(2907..3917)
                     /locus_tag="VIF08_06735"
     CDS             complement(2907..3917)
                     /locus_tag="VIF08_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY8135722.1"
                     /translation="MDFGFTLKPDHTIERTIALTQQAEAGGFSHGWLFDSHVLWKDPY
                     PLLTVMALNSARLRLGTCVTNPATREPSVTASALATLNEISGGRMDLGIGRGDSARRV
                     MGKPPTTLARLEAATNEIRDLVEGRTVNVEGTDLHFTWTRRDKLPVWIAGYGPKALEV
                     TARIADGVILQLADVDLIAWFVGQLRGYAKAAGRDPSAIKVMAAAPAHVGDAGMCRDR
                     VRWFPALVSNHVVDLVNKYDPAELPPGLTAFVRSREGYDYLHHAEVGSNNSEFVTDDV
                     VDRFCVIGSVEQHRQKLAALADAGVDQFNIYLMNGDEEETLATYAREIVPDLSGVVAA
                     RS"
     gene            4171..4587
                     /locus_tag="VIF08_06740"
     CDS             4171..4587
                     /locus_tag="VIF08_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135723.1"
                     /translation="MRTEPPPSTGPAAEIDLLVSGGEFDGSYLAVAANGCASSPADNA
                     FTVAYSNESAADGFVALHLVLRDATQAQEDSSNDFLLELSLTGAGVGPSYTIDPADGE
                     GEGDAFLDITDEEATLDLSVITPEAIIDLTVICELG"
     gene            complement(4584..5765)
                     /locus_tag="VIF08_06745"
     CDS             complement(4584..5765)
                     /locus_tag="VIF08_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8135724.1"
                     /translation="MAVTSPRRVDQLRAAIAPFLGFFNGPYSAMEKDPEVANFAVGNP
                     HELAMPAYVKAIRDSLEPRDKDWYAYKLSEPNAQHTVARSMSALTGLDWDPANVQMTN
                     GGFAAIAVALRTLLEPGDEAIFLSPPWFFYEQLILAADGIPVRVRFDMPDCKLDLARI
                     EAAITPRTRVVLLNSPHNPSGRVVPLEELRALAELLTSASRRVGHDIYLLSDEPYRRI
                     VFDGLTYHSPAEVYPATVVTYSYGKQLLAPGMRIGYLTWPPNMPDRDRLRDDAFTQQL
                     AAGFSFPNADLQHAIADFEPLCIDIAALERRRARLVGKLRGMGYQATWPQGTFYVMAA
                     SPMADDEEFTRILNRHKVLVLPGTVVETPGWFRISLTANDEMVERAIPAFEAALAEAS
                     A"
     gene            complement(5777..6628)
                     /locus_tag="VIF08_06750"
     CDS             complement(5777..6628)
                     /locus_tag="VIF08_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY8135725.1"
                     /translation="MARIVRGALLQASWTGDKESMIQKHEKYAHEAAAQGAQVMLFQE
                     LFYGPYFCQVQDPQYYSYTELIPDGPTTKRMQDLAKQTGMVLVVPMYEEDDQAAGIYY
                     NTAAVIDADGKYLGKYRKTHIPHVKGFWEKFYFRPGNMGYPVFETAVGKVGVYICYDR
                     HFPEGARALGLNGAEMVFIPSATSRGLSEYLWRVEQVSHAVANGYFVGTINRVGIEKD
                     LGEDDFYGQSYFVDPRGQFIGNVGDAHNEELLVRDMDLDMIHEVRNTWQFFRDRRPDA
                     YGDLVRP"
     gene            complement(6704..>7250)
                     /locus_tag="VIF08_06755"
     CDS             complement(6704..>7250)
                     /locus_tag="VIF08_06755"
                     /inference="COORDINATES: protein motif:HMM:NF020075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY8135726.1"
                     /translation="GRIERVPFRKYLAVVLGKEWPGYLPHSVIEAGAVAAKQFAWYHV
                     LQGPRRTRDGRCFDVRDGIQDQLYKPGKSRVRPDHYDAIDATWHITLIKSERFFMTGY
                     RRGDKGRCGRDANGWKLFARTATSCAYKGKDFLEILRIYYGPELTIRRDGERLSEGGS
                     SSAQTASFAPAPTPSVLLNVL"
BASE COUNT         1165 a         2342 c         2523 g         1220 t
ORIGIN      
        1 agatgacgag gtctgcatcg gcacccgggg cgacgacgcc cttgactccg gcgaggccga
       61 acatgcgcgc cggcgccgcc gacacgatct cgacccagcg ctcccgcgtg atgcggccgc
      121 cgaccacgcc gccgtcgtgc agcaggtcga cgcgatcctc cacgcccggc aggccgttgg
      181 gcaccttgcg gaagtcgccc acgcccagct gcttctgcgt ctcgaagtcg aacgggcagt
      241 ggtcggtcga gaccacctgg aggtcgtcct tgaccaagcc gtgccagaga tcgacctggt
      301 ggtcggccgg gcgtagcggc ggtgagcaga cgaacttggc gccattgaag ccgttggcca
      361 tgtcgtcgag cgacaggaag aggtactgcg ggcacgtctc cgcaaaagcc atcgcgccgc
      421 gatcgcgcgc ctcgcgcacc atctgcagcg cgtcgcgggc cgagagatgg acgatgtaca
      481 ccgggacgcc cgccgcctcg gccaggcgga tcacgcgatt ggtcgcctcg ccctcgaaga
      541 ttggatagcg cacgacgccg tggtagtacg ggtcggtctt gccgtccgcg accgcctggg
      601 ccgcgacgac atcgatggcg aggccgttct ccgcgtgcat catgatcagg ccgccgttct
      661 tcgcggtctg ctgcatcgcc cggaagatcg cgccgtcgtc ggagtagaag acgcccgggt
      721 aggcggtgaa gagcttgaag tcggtcacgc cttccgcgac gagctggtcc atctctgcca
      781 gggtcgcgtc gttgacgtca ctcatgatca tgtggaagcc gtagtcggtc gtggcgttgc
      841 cctgcgcctt ggcgtgccag gcgtcgaggc cctcgcgcag cgacttgccc tgtgactgca
      901 ccgcgaagtc gatgatcgtc gtcgtcccac cgaaggccgc ggcccgtgtg ccgctctcga
      961 acgtgtcctt ggcgaaggtg ccgccgaatg gcagctccat gtgggtatgg acatcgatcg
     1021 cgcccgggat gacgtacttg cccgtcgcat cgatcgtctg ctcgacccct tgcgccaggg
     1081 cgctgccggc caggccccga ccaacagcca tgacctgctc gccatcgacc agcacgtcgg
     1141 cgacggccga cccgtcggcg ttgacgaccg tgccgttagt gataaggctc ttcatggccc
     1201 aaatgttacg gcgcgcgtgc tagggcgcgg cgaacgcctc gtagtcgatc gtgatgaccg
     1261 cgccctcctg ggtcgcgtcg ctgcactcga agaacgggtc gggcgtcagc ggatcttcat
     1321 agcaggagaa ctcatagcgc agcgtgcggc cctggttggc ctggtagggc ggcacgaagt
     1381 ccatcgagcc ggtcgggcag ctgccgggca ggcaggtcgc ctggtagacg agctggttct
     1441 tgttgaacag cttcgcggtg ccccactgga cccactggtc gatcgtcacg tgacggatgc
     1501 accacgtctt gggcagcggc ttggcagtcg ggtgccccag gccaagctgg aactgcttca
     1561 ccgcggtgcc ctgcgatgcc agcgcgcaat ccacgggcgt gggcgccggt gtgggcacag
     1621 gagtcggcgt gggcgcgggg gaaggtgtcg gggtaaaggt cggcaggaag gacggcgagc
     1681 ccgtcgcgat gagcgtgggt gtgggcggca cggtgggtga gaacgcggcg gaaggtgttg
     1741 gccccgacgc caggggcgtg gccgttggca cgacggcgat gttggtgggc gtcgcccccg
     1801 ggcggctgtt gcccaagagc cagatcgccc caccaccgag caccagcccg gccaggatca
     1861 gcacggccag cccgagcaac agctgaggca caccgactga cgacgctgcg tcccgattac
     1921 ggtagtagac gccctcgctg agcaggcgca caccgcaacg gttgcagaag acagcgtcgg
     1981 ctggattctg cctccgacac cgggggcaga tgacgaaatc agccacttac gtcaatggta
     2041 gacgcgcgag acggccggtt taggacgcga aactcccaac cggcgtagga gtcgcagtgg
     2101 gctccggggt ttcggtcgga gctggtgttg gcgttggggg cgcctggaac acgactttct
     2161 tctgcttgaa gtactgctgg ccgtcagtgc ccgtcacgac gagcgtcacc gtgtactcgt
     2221 tcggaccaga ggacgggtac tcgtgcgtgg ggttccgctc agtgaccggt gggctgccgt
     2281 cgccgaagtc ccactgccgc gattcgatag gcccggagga attgtcgctg cacgtgatct
     2341 tgcgctgggc tcccagctga gcgcacgtga acttgaccac gaccgcggcg gcggtgggcg
     2401 ggttggtcgg ccgtatgggc gtgggtggtg cggtcgggcc aatggccgtg ctcgacggga
     2461 gcgcggagaa ggatggtgat tcggacggca ggaggatgaa cgtcggcgat gggctgggag
     2521 tggcagtcgg ctgcacgaac ggcggcaggg ttggggagcc ggaagcgagg gcaatgtcgg
     2581 tcggatcggc cggtccgggc ggattggaca ggtagatcgc cgccgcgccg cctatgaaga
     2641 gaaagccggc cagcagcgcg accgcgagca cgaccgcgcc gctcccgcca ccgccgcgtg
     2701 gctcgaccac cgccgtgcgg cggtggttgc cagtgagctt ggtggcaagc ggcaggcgct
     2761 gacggcagcg gctacagaag gtggcgcccg cagagttgcg ccgcccacag ttcggacaga
     2821 tcaagacagg tttcccatgc gcgcacggat caggctgggg cgtgcaagta tcccatgatt
     2881 aggacgaacg cccgccctgg atcgtttcag gagcgcgcgg ccactacccc gctcaggtcg
     2941 ggcacgatct cgcgcgcgta cgtcgcgagc gtctcttcct cgtcgccgtt catgaggtag
     3001 atgttgaact ggtcgacgcc cgcatcagcg agcgcggcga gcttctggcg gtgctgctcg
     3061 accgagccga tcacgcagaa gcgatcgacc acatcgtcgg tgacaaattc cgagttgttt
     3121 gagccgacct cagcgtggtg caggtagtcg tagccctcgc gactgcgcac gaaggcggtc
     3181 agaccgggcg gtagctcggc cgggtcgtac ttgttcacga gatcgacgac gtggttgctg
     3241 acgagcgccg ggaaccagcg cacgcgatcg cggcacatgc cggcgtcacc cacatgggcc
     3301 ggcgccgcgg ccatgacctt gatcgcactc ggatcacggc ccgctgcctt tgcatagccg
     3361 cgcagctggc ccacgaacca ggcgatgaga tcgacgtcgg cgagctgcag gatcacgccg
     3421 tcggcgatac gcgcggtgac ctcgagcgcc ttgggcccgt agcccgcgat ccagacgggc
     3481 agcttgtcgc gcctcgtcca ggtgaagtgc aggtccgtgc cctcaacgtt gaccgtgcgg
     3541 ccctcgacca ggtcacggat ctcgttggtc gcggcctcca ggcgggccag ggtggtcggc
     3601 ggcttgccca tcacacgccg tgccgaatcg ccgcgaccta tgcccaggtc catccggccg
     3661 ccactgatct cgttgagcgt cgcgagcgca ctggcggtga cgctgggctc gcgcgtcgcg
     3721 ggattggtga cgcacgtccc gagtctgagc cgcgcgctgt tgagcgccat gacggtcagc
     3781 agcgggtagg gatccttcca gagcacgtgc gagtcgaaca gccagccgtg gctgaagccg
     3841 cccgcctccg cctgctgggt cagggcgatc gtccgctcga tcgtgtgatc gggtttgagc
     3901 gtgaatccga agtccatgcg cctcttgtcc ctctgcgcgc ttttcgaagg cggaaggata
     3961 cgccccgact cgccgcgcgc agccgttacg gatactcgtc aatgcgcgat gctagacgcg
     4021 atgcatccgc ccttggccgc ttctcgcata gccatgtttc ttgcgtcgct tgcgctgatc
     4081 tgcgcctgcg gagtggctcc cgcgcccgat gccacgccga gtccggtcag cacgggtccc
     4141 agcagcccgc ccggcccgac accctcagcg ttgcggactg agccgccgcc ctccaccggg
     4201 ccggcggccg agattgacct cctggtcagt ggcggcgagt tcgatggctc ataccttgct
     4261 gtcgcggcca acggctgcgc cagcagcccg gcggacaacg cgttcacggt cgcgtattca
     4321 aatgagtccg cagccgatgg cttcgtcgcg ctccatcttg tgctgcgcga cgcgacccag
     4381 gcgcaggagg actcgagcaa cgatttcctg ctcgagctga gcctcaccgg cgcaggcgtc
     4441 ggaccgagct acaccatcga tcccgccgac ggcgagggcg agggcgacgc cttcctggac
     4501 atcaccgacg aggaggcgac gctcgaccta agcgtgatca ccccggaggc aatcatcgac
     4561 ctgacggtca tctgcgagtt gggctaggcg ctcgcctccg cgagagcagc ctcgaaggcg
     4621 gggattgcgc gttcgaccat ctcatcgttc gcggtgaggc tgattcggaa ccagccgggc
     4681 gtctcgacga cggtgccggg caggaccagc accttgtgcc tgttgaggat gcgcgtgaac
     4741 tcctcgtcgt cggccatggg ggaggcggcc atcacgtaga acgtgccctg gggccatgtc
     4801 gcctggtagc ccatgccccg cagcttgcca acgagccgag cccggcgccg ctccagcgcg
     4861 gcgatgtcga tgcacagcgg ctcgaagtcc gcgatcgcgt gctggagatc cgcgttgggg
     4921 aacgagaatc ccgccgcgag ctgctgcgtg aaagcgtcat cgcgcaggcg gtcgcgatca
     4981 ggcatgttgg gcggccacgt caggtaaccg atccgcattc ccggcgccag aagctgcttg
     5041 ccgtacgagt acgtgacgac agtggcggga tagacctccg ccggcgagtg gtaggtcagc
     5101 ccatcgaaga cgatgcgccg gtacggctcg tcactgagca ggtagatgtc gtggcccacg
     5161 cggcgtgagg cgctggtgag cagctccgcc aacgcgcgca gctcctcgag cggcacgact
     5221 cggccgctcg ggttgtgcgg cgagttcaac aggaccacgc gggtgcgcgg cgtgatggcc
     5281 gcctcgatac gcgccaggtc gagcttgcag tcgggcatgt cgaagcgaac cctcaccgga
     5341 atgccgtccg cggccaggat gagctgctcg tagaagaacc acggcggcga gaggaagatc
     5401 gcctcgtcgc ccggctccag gagggtccgc agcgcgacgg cgatcgcggc gaagccgccg
     5461 ttggtcatct ggacgttggc cgggtcccag tcgagcccgg tgagcgctga catggagcgc
     5521 gccacagtgt gctgggcatt gggttcggac agcttgtagg cgtaccagtc cttgtcgcgc
     5581 ggctcgaggc tgtcgcggat cgccttgacg taggccggca tggccagctc gtgcgggttg
     5641 ccgacggcga agttggcgac ctcggggtcc ttctccatgg ccgagtacgg gccgttgaag
     5701 aaacccagga acggcgcgat cgccgccctg agctgatcga cccggcgggg cgacgtcaca
     5761 gccatgcgct agatgttcac gggcggacga gatccccgta ggcgtctggc cggcggtcgc
     5821 ggaagaactg ccacgtgttg cgcacctcgt ggatcatgtc gaggtccatg tcgcgcacga
     5881 gcagctcctc gttgtgcgcg tcacccacgt tgccgatgaa ctggccgcgc ggatcgacga
     5941 agtacgactg gccgtagaag tcgtcctcgc ccaggtcctt ctcgatgcca acgcggttga
     6001 tcgtgcccac gaagtagccg ttggccacgg cgtgtgagac ctgctcgacg cgccacaggt
     6061 actccgacag gccacgcgag gtggccgagg ggatgaacac catctcggcg ccgttgaggc
     6121 ccagtgcgcg ggcgccttcc gggaagtgcc ggtcgtagca gatgtacacc ccgaccttgc
     6181 cgaccgcggt ctcgaacacc gggtagccca tgttgcccgg gcggaagtag aacttctccc
     6241 agaagccctt cacgtgcggg atgtgcgtct tgcggtactt gcccaggtac ttgccgtcag
     6301 cgtcgatcac cgcggcggtg ttgtagtaaa tacccgccgc ctggtcatcc tcctcgtaca
     6361 tcggcaccac caggaccatg cccgtttgct tggccaggtc ctgcatccgc ttggtggtcg
     6421 gcccgtcagg aatgagctcg gtgtacgagt agtactgcgg atcctgcacc tggcagaagt
     6481 acggcccgta gaagagctcc tggaagagca tcacctgggc gccctgcgcg gccgcctcgt
     6541 gggcgtactt ctcgtgcttc tggatcatcg attccttgtc gcccgtccag gaagcctgga
     6601 gcagcgcgcc gcggacgatt cgggccatct cgactcacct cggtgtgacg tggatctcgg
     6661 cgttttctgg gcgctgaggg tacaccgagg tgccgactac gggttagagg acgttgagca
     6721 gcaccgaggg cgttggggcc ggggcaaatg acgcggtctg ggcgctactg ctgccgccct
     6781 cgctgagcct ctcgccgtca cggcggatcg tgagctcggg accgtagtag atgcgcagga
     6841 tctccaggaa atccttgccc ttgtacgcgc agctcgtcgc tgtgcgcgcg aagagcttcc
     6901 agccgttcgc atcgcgaccg cagcggccct tgtcaccgcg ccggtagccg gtcatgaaga
     6961 accgctcgct cttgatgagt gtgatgtgcc acgtcgcgtc gatagcgtcg tagtgatcgg
     7021 gccggacgcg cgacttgccc ggcttgtaga gctggtcctg gatgccatcg cggacgtcga
     7081 agcaccggcc atccctggtc cggcgcgggc cctgcagcac gtgataccaa gcgaactgct
     7141 tggccgcaac ggcaccggcc tcgatcaccg agtgcggcag gtaacccggc cactccttcc
     7201 cgagcacgac cgcgaggtac ttgcggaagg gcacgcgttc gatgcgtccg
//
LOCUS       DATBRX010000174         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3195470, whole genome shotgun sequence.
ACCESSION   DATBRX010000174 DATBRX010000000
VERSION     DATBRX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3195470"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..249)
                     /locus_tag="VIF08_06760"
     CDS             complement(40..249)
                     /locus_tag="VIF08_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135727.1"
                     /translation="MSFGNPNSRPGAYPPADLRGQGGWNRESYQLPNEGDSRAKYDDA
                     TEKESAGLTKPSLFERFKRWFSAKF"
     gene            complement(270..443)
                     /locus_tag="VIF08_06765"
     CDS             complement(270..443)
                     /locus_tag="VIF08_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135728.1"
                     /translation="MLRLIRRFVGALRDGLKSAVFGPDLPGGINDEALPSGFIAAYKK
                     GSRQVRNLGHIDF"
     gene            541..1062
                     /locus_tag="VIF08_06770"
     CDS             541..1062
                     /locus_tag="VIF08_06770"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY8135729.1"
                     /translation="MSADSLRAKIREIPDFPKPGILFYDITTLLKEPDAFKEAIKLML
                     DPFRDKQIDVVVGMESRGFIFSAPMAIELGAGFVPVRKLGKLPAETASVEYALEYGTN
                     TLEIHKDALKPTQKVLIVDDLLATGGTVLGTIELVKMLKAEVVGLAFLVELLFLKGRE
                     RLGEYTTHSVIQY"
     gene            1165..2361
                     /locus_tag="VIF08_06775"
     CDS             1165..2361
                     /locus_tag="VIF08_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135730.1"
                     /translation="MARPDEQDTSRRDFFRTFGRETVRQAGAVAGAAAELRRSSLAAA
                     RVLFDPEAPTDAPVLETADLSEPVVDKSQPDATFRSAYRFTGTEITALDARQLPGRVQ
                     TVALVEPSEVASAIRAGAINGGPVLAAVGAYAMALAARRAVARPDTGRVQQVRAAANT
                     LRAAKPQIHALTWAVDRLEASYLELEGQGTDPAQVAESLESEADSIASGSAGAHSQIG
                     RIGAAAALSGEGEPINLLMHGDMGPLSCGMVGMGTALIQELRDAGRAVHVWLTEAAPG
                     GEGGRVAAFGLGQLDVPFTVIPDAAVGWLIDRHSVAALLLRGDRVAANTDCGVLVGGL
                     TAAIVAAAADVPVIVLAPRNSFDPAAADGAALRAGETFAPGARLNPSSDVVPHDLVRQ
                     LVSEAG"
     gene            2364..>3133
                     /locus_tag="VIF08_06780"
     CDS             2364..>3133
                     /locus_tag="VIF08_06780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135731.1"
                     /translation="MATATQHGRARTQERAIVAAPTRDKQLLRAFLERDRLRAGYAVA
                     DLEEREFAKSRWGVASEGGQPIAVALEYGGLTPQPLFVVGDPDGIVAILRDVIRPRLA
                     YLAADESHLAAVADLYRIDAGPPMVRMAVDRRTFEPVAGPVQRLAPADIVDLNRLYGL
                     GFAGWLPADSIANGIYYGVRIAGRLVAAAGTHVISPEGRIAAVGNVMTHIDFRGRGYA
                     KQTTSAVTQELLRTCDDVVLNVRADNPPALAAYRALGYR"
BASE COUNT          474 a         1077 c         1077 g          505 t
ORIGIN      
        1 tcccggtcct gggctacggg gagcccgccg cctagcgcct cagaacttcg cggagaacca
       61 gcgcttgaac cgctcgaaga ggctcggctt ggtcaaccct gccgactcct tttccgtggc
      121 atcgtcgtac ttggcgcgtg agtcgccttc atttggcagt tggtagctct cacggttcca
      181 gcccccttgg ccgcgcaaat cagccggtgg gtaggcaccg ggccgcgagt tcggattgcc
      241 gaagctcatt ttcgatgcct ccgtcgtgcc tagaagtcga tgtgccccag gttgcggacc
      301 tggcgcgagc ccttcttgta cgccgcgatg aaacctgacg ggagggcttc gtcgttgatt
      361 ccgcccggca gatccggtcc aaacaccgcg ctcttcaacc catcgcgaag cgcaccgacg
      421 aaccgtctga tgagtctcaa cacacattta cgctagcacg atcgctgcac gggcctgcgc
      481 tagactgacg cggccctggt cggctttagt cgaaccgtca gtgaggaccc tgtaagcacc
      541 gtgtcggcag actccctgcg cgccaagatc cgcgagatac ccgacttccc caagccgggc
      601 atcctcttct acgacatcac caccctgctc aaggagccgg acgccttcaa ggaagcgatc
      661 aagttgatgc tcgatccctt ccgcgacaag cagatcgacg tggtggtcgg catggaatcg
      721 cgcggcttca tcttcagcgc gccaatggcg atcgagctcg gcgccggctt cgtgccggtc
      781 cgcaagctgg gcaagctgcc ggccgagacg gcgagcgtcg agtacgcgct ggagtacggc
      841 acgaacacgc tcgagatcca caaggacgcg ctcaagccca cacagaaggt gctcatcgtc
      901 gatgacctgc tcgcgactgg cggcacggtc ctcggcacga tcgagctggt caagatgctc
      961 aaggccgagg tcgttggcct cgcgttcctg gtcgagctgc tcttcctcaa ggggcgcgag
     1021 cgactcggtg agtacacgac ccacagcgtc attcagtact gacgcgtcag ctcgactgtc
     1081 tgcgcagcga cgcctcacgg ctgctgctgt gcactccgtg aaggcccctg cggggcaggt
     1141 gccactccgc ttgcgggcgg tctgatggcc cgccccgacg agcaggacac gagccggcgc
     1201 gatttcttcc gcacgttcgg acgcgagacc gtgcgccagg cgggcgccgt cgcgggcgcg
     1261 gccgctgagc tgcgccgctc cagcctcgcg gcggcgcggg tgctcttcga tccggaggcg
     1321 ccgaccgatg cgccggtcct ggagacggcc gacttatcgg aacccgtcgt cgacaagtct
     1381 cagcctgacg cgaccttccg cagcgcctat cgcttcaccg gaacggagat cacagccctt
     1441 gacgctcgcc aattgcccgg ccgcgtgcaa acggtcgcgc ttgttgagcc gagcgaggtc
     1501 gcatctgcga ttcgggccgg cgcgatcaac ggtgggccgg tgctcgccgc ggtgggcgcg
     1561 tacgcgatgg cgcttgccgc acgccgtgct gtcgctcggc cggacacggg ccgcgtccag
     1621 caggtgcgcg ccgcggcgaa cacattgcgc gccgcgaagc cgcagatcca cgccctgacc
     1681 tgggcggtcg accggctcga ggcgtcctac ctggagctcg agggccaggg caccgatccg
     1741 gcgcaggtgg ccgagagcct tgagtcagag gcggattcga tcgcttcggg cagcgccggc
     1801 gcgcattcgc agataggccg gataggtgcc gcggcggcgt tgtccgggga gggcgagccg
     1861 atcaacctgc tcatgcacgg cgacatgggg ccgctgtcat gcggcatggt gggcatgggc
     1921 actgcgctca tccaggagct gcgcgacgcc ggccgagcag tgcacgtctg gttgacggaa
     1981 gcggcgccgg gcggcgaggg cggacgcgtg gccgcattcg ggttgggcca gctcgacgtg
     2041 ccgttcaccg tcatccccga cgcggccgtg ggttggctga tcgatcgcca cagcgtcgcc
     2101 gcgctcctgt tgcgtggcga tcgagtcgcg gcaaatacag actgcggcgt gctcgttggc
     2161 gggctgacgg cggcgatcgt cgccgcggcc gcggacgtgc cggtgatcgt cctcgcgccg
     2221 cgcaacagct tcgatccggc cgcggcagat ggcgcggcac tccgcgccgg cgagacgttc
     2281 gctccggggg cgcgcctgaa cccgtcgagc gacgttgtgc cccatgacct ggtcaggcag
     2341 ctggtcagcg aggcggggta gcgatggcga ccgcgaccca gcacggccgc gcccgtacgc
     2401 aggaacgggc catcgtggcg gcgccaacgc gcgacaagca gctgctgcgc gccttcctcg
     2461 agcgcgaccg gctgcgcgcc ggctatgcgg tggcagatct cgaggagcgc gagttcgcga
     2521 agtcccgttg gggagtggcg agtgagggcg gccagccgat cgcggtcgct ctcgagtacg
     2581 gcggcctgac gccccagccg ctcttcgtcg tgggcgaccc agatggcatc gtcgcgatcc
     2641 tgcgcgacgt gatccggccg cgccttgcgt acctcgccgc ggacgagagt cacctggcgg
     2701 cggtggccga cctgtaccgc atcgacgccg ggccgccgat ggtgcgcatg gcggtcgatc
     2761 ggcgaacgtt cgagcccgtg gccggtccgg tccagcgcct ggcaccggcc gacatcgtcg
     2821 atctcaatcg gctgtacggc cttggtttcg ccggctggct gcccgccgat tccatcgcca
     2881 acggcatcta ttacggagtg cgcatcgccg gccggctcgt cgccgcggcg ggcacgcacg
     2941 tgatcagtcc cgaaggccgg atcgccgccg tcggcaacgt gatgacgcac atcgacttcc
     3001 gcgggcgcgg ctacgccaag cagaccacga gcgcggtcac ccaggagctg ctgcgcacct
     3061 gtgacgacgt cgtgctcaac gtgcgcgcgg acaacccacc ggcgctggcc gcctaccgcg
     3121 cgctgggtta tcg
//
LOCUS       DATBRX010000175         7175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_961493, whole genome shotgun sequence.
ACCESSION   DATBRX010000175 DATBRX010000000
VERSION     DATBRX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961493"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VIF08_06785"
     CDS             <1..640
                     /locus_tag="VIF08_06785"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY8135732.1"
                     /translation="PSNAWTLIKALMWQGDMCFAPSIGGQPTFQRLDAIPRWPGLPDL
                     DEAGRRAITAYLSTYGPATLDHLQYWLGAGLSAGRKRINDWFAGLGAGGRLAEVEVGG
                     ELAVVLREDVDELLAARSTAAVRLLPAYDQWVMGPGTADPNVVPVARRALVSSGANVA
                     LVGGVVAGTWTLKGDVVGIDWFEPRKAGASAAVADEIKRLGRILGRPVSADG"
     gene            633..1094
                     /locus_tag="VIF08_06790"
     CDS             633..1094
                     /locus_tag="VIF08_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135733.1"
                     /translation="MVDRLPAPGISFLKEEMVSLRDGTKLRAGMTNPSVMDDGWWLTN
                     LWVGDDGGVVPALSAAPAAGPPPGTPLEALGPRVAGALSGLIAEAGGRTQIRLRMPPA
                     EDEARPWERPLICAVAIQWDAVRAAVMTKNQLARELLRGFAAAVEALGRPG"
     gene            1139..1975
                     /locus_tag="VIF08_06795"
     CDS             1139..1975
                     /locus_tag="VIF08_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135734.1"
                     /translation="MEITLAPERLFLLEERLTIDEITQRAMDKRTSALGSLLSKPKPE
                     EVTLVNKQRRLEPFWHVAGHAIYVYERQRDYSVPAASADVEALTILGTRYEGQAGAGA
                     RAFRVPALEHCRTEIRSESFVDGLTGAAVADGPQVTEGPRSEVADPATLAEGDTIAMP
                     PENRASYVVRALLGELIKPVQADKLLEEEVVLEATDLFYRPVWAFEFTWNGKGKNAIV
                     EIDSVTGQTRAGKALVGQIRGALNRDLLFDVGADTVGLFVPGGSIAVKLAKAAMDSNK
                     KK"
     gene            2114..2935
                     /locus_tag="VIF08_06800"
     CDS             2114..2935
                     /locus_tag="VIF08_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135735.1"
                     /translation="MLRGGLVVLLVPIFVLPTQVEVRLMLGGNLGSTGFSPALWTAVG
                     GATIFSTGLVLVVLFALAWLEAATFALLASDPDLEIPVRVDPAARVRELFVVQSLTLI
                     ALLLAAVPLASALGQVTYDEILRPTSTGAIFDRVLAQVLLPLSLLAAALPIIDSVSAA
                     ATRRLLIGRSVGSAVSGSIGALVRRPIRFLATAAVAWLALAAVVVATETALGIAWQQT
                     RAAFLRTTSIADMLASLAPLLVAVLLCGVFASGLAVCGYVAAFRNALWTITSLRR"
     gene            2992..3639
                     /locus_tag="VIF08_06805"
     CDS             2992..3639
                     /locus_tag="VIF08_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8135736.1"
                     /translation="MIEQLLQTLGDEINKFLDSPLVRLTIQAVAFYVVLLWAATAYWA
                     YRDLQQRTSNPVAPYLAAALIILFTPVFFLFGVIIYRILRPSETVAQANERALAEEAM
                     MVEIESQPHCANCSRQVHEDWIICPTCRNRLRRVCPNCSRLVELDWSLCAWCGKDFER
                     PNARPEAVQPELEPAPRRRGGAQADAPTEADALPAPQMSVPSIPASPLPSRQTSE"
     gene            3662..4594
                     /locus_tag="VIF08_06810"
     CDS             3662..4594
                     /locus_tag="VIF08_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEY8135737.1"
                     /translation="MTLADRPAAGLYVAAWALCGLGVAGLVIGLASGPPARGFLIMGA
                     LVALILGLATAAGQQLVARRSRPAERYRGPSPLILFFLQLVLVSSISIVLFTVGVPLD
                     TPGGFLVAATLLLSGYLFVVWLFVVRGGALSWREMIRAEPLDASRATFDIVIGGATMF
                     GVAIAAAIIGGVIARLLGTSVPEVVPSPQTAPEILMAVLAAGLLVPIGEEVFFRGFTM
                     TAWLRDLGPRTALIRATVFFALVHVLNITTETFSEGLAQAILEVAVIAPVGLALGWLY
                     LKRGLIAAIGGHAAFNVLGVLILVLAQNLPAPGT"
     gene            4800..5456
                     /gene="radC"
                     /locus_tag="VIF08_06815"
     CDS             4800..5456
                     /gene="radC"
                     /locus_tag="VIF08_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY8135738.1"
                     /translation="MREVPVSERPRERLAAHGPAALSASELLTVLWGSRGPAETTASH
                     SDLSDLAGSDLHELTSIPGVGEARAAQLMVAFEIGRRSQAGGRGERWTIRAPRDVADR
                     LLPEMARLEREELRVLLLNAKNGVIRQSTVYVGNVSGALVRVAELFRDAVRAHAAGLI
                     IVHNHPSGDPEPSPDDLHLTAEAIAAGRLLDLPLLDHVILAADGFISLRDRGIAFDRR
                     "
     gene            5484..6359
                     /locus_tag="VIF08_06820"
     CDS             5484..6359
                     /locus_tag="VIF08_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8135739.1"
                     /translation="MAPAEREFRAPTEGGEIVGWIRDAPGKPNALVLHGGPGLSEYLD
                     TLVAELDGLVTTARYQQRGLAPSVVSGDISIEGHIADAVAVMDALGWDRPLVIGHSWG
                     GHFAMHLAVAHPDRVGGLVILDTLGAVGDGGIAEFGPNLMRGLSEGAQDRIADLNAQE
                     ENGEPLTEEERREHLELLWPNYFGDPASAPPMPPLRFGRADETWKSIMANFESHTLEH
                     MLPTVTFPTLVIHGGKSPIPVAEAHRIAALMPNARLVVHEGNGHWPWLEEPGFVRAQL
                     AAWLPRSVVEPSAEA"
     gene            6603..>7175
                     /gene="mreB"
                     /locus_tag="VIF08_06825"
     CDS             6603..>7175
                     /gene="mreB"
                     /locus_tag="VIF08_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HEY8135740.1"
                     /translation="MLDAFLGMFSRDIGIDLGTANTLVHVKDRGIVISEPSVVAIDQK
                     TRRVLAIGAEAKRMLGRTPASIIAVRPLRDGVISDFDVTEQMIKYFVHKVHDRVGLIP
                     RPRMLIGIPSGVTEVEKRAVRDAAINAGARWARLIEEPMAAAIGAGLPVAEPTGSLIV
                     DIGGGTTEVAVISLGGIVVSRSIRIGGDELD"
BASE COUNT         1048 a         2457 c         2480 g         1190 t
ORIGIN      
        1 cccaagcaac gcgtggacct tgatcaaggc gctcatgtgg cagggggata tgtgtttcgc
       61 gccctctatt ggcggccagc cgacgttcca gcgcctggac gccataccgc gctggccggg
      121 gttgccggat ctcgacgagg ccggccggcg cgcgatcacg gcctatctct cgacctatgg
      181 gccggccacg ctcgatcacc tgcagtactg gcttggtgcc ggtctgagcg ccggacgcaa
      241 gaggatcaac gactggttcg ccgggctggg cgcgggcggc cgcctggcgg aggtcgaagt
      301 cgggggcgaa ctggcggtgg ttctgcgcga agacgtggat gagcttctcg ccgcgcggtc
      361 aaccgccgcg gtccggctgc tgcctgcgta cgaccagtgg gtgatgggtc ccggcacggc
      421 cgaccctaac gttgtgcctg tcgcccggcg ggccctcgtc agcagcggcg ccaacgtcgc
      481 gcttgtgggc ggcgtcgttg ctgggacatg gacccttaag ggcgacgtgg tcggcatcga
      541 ctggttcgag ccgcgcaagg ccggcgccag cgcggcggtt gccgacgaga tcaagaggct
      601 gggcaggatc ctgggccgcc cggtcagcgc cgatggttga ccggctgccc gcgccgggca
      661 tctcgttcct taaggaagag atggtcagcc tgcgcgacgg cacgaaactg cgcgcgggca
      721 tgaccaaccc cagcgtcatg gacgacggct ggtggctcac caacctgtgg gtcggggacg
      781 atgggggagt ggtgccagcg ctgagcgccg cgcccgccgc cggcccgccg ccgggaacac
      841 cgctcgaggc gctggggcca cgggtggcag gcgcgctcag cggattgatc gccgaggccg
      901 gcggccgcac tcagatccgg ttgcgcatgc cgccggcgga ggacgaggcg cgaccgtggg
      961 agcggccgct catctgcgcg gtcgcgatcc aatgggatgc cgtccgcgcg gcggtgatga
     1021 cgaagaacca actggcgcgc gagctattgc gtggcttcgc cgccgcagtc gaggcattgg
     1081 gccgccccgg ctgagacaac ccctaccgct ccgcgctcgg gcggtgcatc atgccgcgat
     1141 ggagatcacc ctcgcccccg aacgcctgtt cctccttgaa gagcgcttga cgattgacga
     1201 gatcacgcag cgggcgatgg acaagcgcac gtctgcgctg ggctcgctgc tctccaagcc
     1261 caagcccgaa gaagtgacgc tggtcaacaa gcagcgccgc ctggagccgt tctggcacgt
     1321 ggccggccac gcgatctacg tctacgagcg ccagcgcgac tacagcgtgc ccgcggcctc
     1381 ggccgacgtc gaagcgctga cgatcctcgg gacgaggtac gagggccagg ctggcgccgg
     1441 ggcacgtgca ttccgtgtgc cggcgttgga gcactgccgc accgagatca ggagcgagtc
     1501 gttcgttgac ggcctgaccg gggctgctgt cgccgatggg ccgcaggtca ccgaggggcc
     1561 acgctccgag gtcgccgacc cagccacgct cgccgagggc gacacgatcg cgatgccgcc
     1621 agagaaccgt gcgtcctacg tcgtgcgcgc gttgctgggc gagctgatca agccggtcca
     1681 ggccgacaag ctgctcgagg aagaggtcgt gctggaggcg acggacctct tctatcggcc
     1741 ggtctgggcg ttcgagttca cgtggaatgg caaaggcaag aacgcgatcg tcgagatcga
     1801 ctctgtcacc ggccagacgc gcgccggcaa ggcgctggtc ggccagatcc ggggcgcgct
     1861 gaaccgcgac ctgctgttcg atgtcggcgc cgacacggtg ggcctgtttg tgcctggcgg
     1921 cagcatcgcg gtcaagctgg ccaaggcggc catggacagc aacaagaaga agtaggtccc
     1981 gatcagcagc accatcctcg ccgcaccggg tgtgcggacg gctcccaagg ctggctggcg
     2041 tgcgcaggtc ggccatgcgc tggcagcgcc ggtcgagcac cctcagctgt ggctcctcgg
     2101 aacactgagc ttcatgctgc gtggcggcct cgtcgtcctg ttagtgccga tcttcgtctt
     2161 gccgacgcag gtcgaggtgc gacttatgct cggcggcaac ctgggctcca ccggcttctc
     2221 gccggcccta tggacagccg tgggcggggc gaccatcttc tcgacggggc tcgtgctggt
     2281 cgtgctgttc gccctggcct ggctcgaggc ggccacgttc gcgctgctgg caagtgatcc
     2341 ggacctggag atcccggtgc gcgtggaccc ggcggccagg gtgcgcgagt tgttcgtcgt
     2401 ccagtcgctg accctgattg cgctgctgct cgcggctgtc ccgctcgcct cggcgctggg
     2461 acaggtcacg tacgacgaga tcctgcgccc aacgtccacg ggggccatct tcgaccgcgt
     2521 gctcgcacag gtcctgctgc cgctcagtct gctcgctgcc gcgctgccga tcatcgattc
     2581 ggtgtccgcc gcggcgaccc gccggctgct cattggcagg tcggtcggat cggccgtctc
     2641 ggggtcaatc ggtgccttgg tccgccggcc cattcgcttc ctcgccacgg ccgcagtcgc
     2701 gtggctggcc ctggccgccg tcgtcgtggc aacagagacc gcgctgggca tcgcctggca
     2761 acagacgcgc gccgctttcc tgcggacgac gtccatcgcc gacatgctgg cgtcgctcgc
     2821 gccactcctc gtggctgtgc tgctgtgcgg tgtattcgcg agcggcctgg cggtgtgcgg
     2881 ctacgtggca gcgttccgca atgcgctgtg gaccatcacc agccttcggc gctgagcgtc
     2941 agagggtaaa ctcggccaac tcgtggactc gaccgatgag gcgcgcggcg catgattgaa
     3001 cagctcctgc agacgctcgg ggacgagatc aacaagttcc tcgactcccc gctggtgcgc
     3061 ctgaccattc aggccgtcgc cttctacgtc gtgctcctct gggcggcgac cgcctactgg
     3121 gcgtaccgcg atctccagca gcgcacgtcc aatcccgttg cgccgtacct ggccgcggcg
     3181 ctcatcatcc tgttcacgcc ggtgttcttt cttttcggcg tgatcatcta ccgcatcctg
     3241 cgcccgtctg aaaccgtcgc ccaggccaac gagagggcgc tcgccgaaga ggcgatgatg
     3301 gtcgagatcg agtcgcagcc acactgcgcg aactgctcgc gccaagtgca cgaggactgg
     3361 atcatctgtc ccacctgtcg caaccggctg cgccgcgtct gtcccaactg ctcgcgcctg
     3421 gtcgagctcg actggtcgct gtgcgcctgg tgcggcaagg acttcgaacg acccaacgcc
     3481 cgacccgaag cggtgcagcc tgagctggaa ccggcaccgc gcagacgagg cggtgcccag
     3541 gcagacgcgc ccaccgaagc cgacgccttg ccagcgccac agatgtcggt gccgtcgatc
     3601 ccggcgtcac cgttgccgtc gcgccagacc tccgaataac cctgcgactg acggcccacc
     3661 cttgactctc gccgaccgtc ccgcggccgg cctctatgtc gctgcctggg cgctgtgtgg
     3721 cctgggcgtc gcggggctgg tcatcgggct tgcgagcggt ccgccggcgc gcggcttcct
     3781 catcatgggc gcgctcgtgg cgctcatcct gggtctcgcc acggccgccg gccagcagct
     3841 ggtcgcgcgc cgctcccggc cggcggagcg atatcgcggc ccatcgccgc tgatcctgtt
     3901 cttcctgcag ctcgtgctcg tcagctcgat cagcatcgtc ctgttcacgg ttggggtgcc
     3961 gctcgacacg ccgggcggct tcctcgtcgc ggccaccctc ctgctgagcg gctatctgtt
     4021 cgtcgtgtgg ctcttcgtgg tgcgcggcgg cgcgctgagc tggcgcgaga tgatccgcgc
     4081 cgagccgctc gacgccagcc gcgccacgtt cgacatcgtc atcggcggcg cgacgatgtt
     4141 cggtgtcgcg atcgcggcgg ccatcatcgg cggcgtcatc gcccggctcc tgggcacgag
     4201 cgtacctgag gtcgtgccgt cgccgcagac agcgccggag atcctcatgg ctgtgctcgc
     4261 cgcggggctg ctggtgccga tcggcgagga ggtctttttc cgtggcttca ccatgaccgc
     4321 ctggctgcgc gacctggggc cacgcacggc gttgatccgc gcgaccgtgt tcttcgcgct
     4381 cgtgcacgtg ctcaacatca cgaccgagac attcagcgag ggcctcgcgc aggccatcct
     4441 cgaggttgcg gtgatcgcgc cggtcggcct ggcgctgggc tggctttacc tcaagcgcgg
     4501 cctgatcgcg gcgatcggtg gtcacgcggc gttcaacgtg ctgggcgtgc tgatcctcgt
     4561 tctcgcgcag aacctgcccg caccgggcac ctgatcggga ttgcggaaac tgcgaaaact
     4621 cggcggcaat tcagcggagg gccggcgcta aggcttcgtg cctagggttc gtctcgctcc
     4681 atggttcggg cttgcgggaa atcggcgggc tcgcttgttt ggaccgtgga gcaccgtttt
     4741 ggaaccgctt cgcgccggtc tacctgtggg tgccgatgaa cgaccgacct gccctacgaa
     4801 tgagagaggt tccggtttcc gaacggccac gggaacgact tgccgctcac gggccggcag
     4861 ccctgtcggc ttccgaactc ttgacggttc tgtggggaag ccgcggaccc gccgagacga
     4921 cggctagcca cagcgacctg agcgatctgg ccggctccga cctgcacgag ctgacctcga
     4981 ttcccggcgt cggtgaggcg cgcgctgccc aactgatggt ggcgttcgag atcgggcggc
     5041 ggtcgcaggc cggaggccgt ggtgagcggt ggacgattcg ggcgccgcgc gacgtggccg
     5101 atcggctgct gccggagatg gcccgcctgg agcgcgaaga gttgcgggtg ctgctcctca
     5161 acgccaagaa cggcgtgatc cgccaatcga cggtctacgt cggcaatgtc tcgggcgccc
     5221 tcgtgcgcgt ggccgagcta tttcgcgacg cggtccgggc ccacgccgca ggcttgatca
     5281 tcgttcacaa ccacccatcg ggcgaccctg agccatcgcc cgacgatctg cacctgaccg
     5341 cggaggcgat cgcggcgggc cgactgctcg acctgccgtt gctcgatcac gtgatcctcg
     5401 cggccgacgg attcattagc ttgcgcgacc gcggcatcgc attcgaccgc cgctgagcgc
     5461 tgccgtgagc ctatgctgcg ggcgtggcac cggctgagcg cgagtttcgg gcgccgactg
     5521 agggcggcga gatcgtcggc tggatccgtg acgcgcccgg caagccgaac gccctcgtgc
     5581 tccatggcgg cccgggattg agcgagtacc tcgacacgct cgttgccgag ttggacgggc
     5641 tcgtcacgac cgcgcgctat cagcagcgcg gcctcgcccc gtcggtggtg agcggcgaca
     5701 tctcgatcga gggccacatc gccgatgccg tagcggtcat ggatgcgctg ggttgggaca
     5761 gaccgctggt gatcggccat tcgtggggcg gccatttcgc gatgcacctc gcggtcgccc
     5821 acccagaccg cgtcggcggc ctggtgatcc tcgacaccct cggcgccgtt ggcgatggcg
     5881 gtatcgccga gttcgggccg aacctgatgc gcggcctttc agagggcgcc caggaccgca
     5941 tcgccgatct caacgcgcag gaagaaaacg gcgagccgct gaccgaggaa gagcgccgcg
     6001 agcacctcga gctgctgtgg ccgaactatt tcggcgatcc agcgagcgct ccgccgatgc
     6061 cgccgctgcg cttcggccgc gcggatgaga cgtggaaatc gatcatggcc aacttcgaga
     6121 gccacaccct cgaacacatg ctgccgacgg tcaccttccc gacgctggtg atccacggcg
     6181 gcaagtcacc catcccggtg gcagaggcac acaggatcgc ggcgctcatg cccaacgcgc
     6241 ggctcgtcgt gcacgaaggc aatggccact ggccgtggct cgaggagccc ggcttcgtgc
     6301 gcgcccaact ggcggcatgg ttgccccggt cagtggttga accgtccgcc gaagcctgag
     6361 taagcgcctc cgatcccgct gtcagaggac gaacgccccg tccagcggtg gaaatggtgt
     6421 ctggacggcc gattgacccc ggcccaatgc acccagcgga cggttgaccc gctcagcggg
     6481 gcaagcgccc cgcgcccggc cggcgaacag tcccctcggg tatacactgc gcaggcttcc
     6541 ggtcccgccg ccttagtcgt cctggcgcgg accactcaca tcaccaggga atcaacgccg
     6601 caatgctcga cgccttcctc ggcatgttct cgcgcgacat cggaatcgat ctcggcacgg
     6661 ccaacacgct cgtccatgtg aaggaccgcg ggatcgtcat cagcgagccg agcgtcgtgg
     6721 cgatcgacca gaagacgcgc cgcgtattgg cgatcggcgc cgaggcgaag cgcatgctcg
     6781 gccgcacgcc ggcgagcatc atcgcggtgc ggccgctgcg cgacggcgtg atcagcgact
     6841 tcgacgtgac cgagcagatg atcaagtact tcgtgcacaa ggtgcacgac cgcgtcgggc
     6901 tcatccccag gccgcgcatg ctgatcggca tcccgagcgg cgtgaccgag gtcgagaagc
     6961 gcgccgtgcg tgacgcggcc atcaatgccg gcgcccgctg ggcgcgactc atcgaggaac
     7021 ccatggcagc cgcgatcggc gccgggctgc cggtcgcgga gccgacaggc tcgctgatag
     7081 tcgacatcgg cggtggcacg accgaggtcg ccgtcatctc actcggcggc atcgtggtca
     7141 gccgcagcat ccgcatcggc ggggacgagc tcgac
//
LOCUS       DATBRX010000176         8030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_67528, whole genome shotgun sequence.
ACCESSION   DATBRX010000176 DATBRX010000000
VERSION     DATBRX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67528"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..2202
                     /locus_tag="VIF08_06830"
     CDS             130..2202
                     /locus_tag="VIF08_06830"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY8135741.1"
                     /translation="MTRIIDFDRPPEGVARQDLPRLLGGKAANLTIMAVDFGLPVPPA
                     FTLTTDVCREYLASGWPPRLEEELREHMARLEELVGRRFGEPADPLLVSVRSGAPVSM
                     PGMMDTILNVGLNAATSAAFDESLAPRLKTRLSESLAAVGVEPVPEDQWGQLRAAIEA
                     VFRSWNSPRAIAYREREGISADMGTAATVQAMVFGNRNERSATGVLFTRNPSTGENRP
                     YGDVLFNAQGEDVVAGTHATEDIRVLHKRLPDVARLLHEYTRTLERHLRDMADIEFTI
                     EDGKLWLLQVRVGKRSPQAALRLAAEMADDPDFPLSREEAVRRVLPLLADPPRVTEKP
                     RDAAEPLTKGLGASPGVAAGEVVTTPEAAVTAAEQGRAVILVRAETSPDDVHGMSVAR
                     GILTATGGLASHAAVVARGWGIPAVVGAAAVSVGSESISVNGRQLKVGDQITIDGSTG
                     EVFGGALAGSSEVVPEAATLLDWARELGIPIEGTDSPASPSAESPGTATPADALRLLL
                     VKGYATPEQAAECLMVSLEDARSLLAGLETSADVEQAVGSYRLTDAGKVRARMDLDAA
                     RAAWPGPVAALDEFVGLDKQVKDVVTSWQMRGESVNDHSDAAYDALVLNGLAELHNEI
                     QAWLPSRFNRYRQRLDRAAAAVAAGDTKFVASPRVDSYHSAWFELHEELILLAGRTRA
                     DEVAAGRA"
     gene            2242..2472
                     /locus_tag="VIF08_06835"
     CDS             2242..2472
                     /locus_tag="VIF08_06835"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY8135742.1"
                     /translation="MSRIRTNIEIDDADVRRIMARYSLKTKTEAVALALRTLAGQPMT
                     LEEALAMQGSHAIGEVPEDCGPHGIDDRPAPR"
     gene            2469..2882
                     /locus_tag="VIF08_06840"
     CDS             2469..2882
                     /locus_tag="VIF08_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEY8135743.1"
                     /translation="MILADTSAWIEYIRGTESPVHQRLRDLVLGADQLVVTEPVLMEV
                     LGGARNDDSETALRRLLLRCDFVPFLSATDFEGAAAVYRHCRASGVTPRGFVDCMIAT
                     VARRVGATVLAHDVDLARIASVIGLELDPASLAAS"
     gene            complement(2879..3832)
                     /locus_tag="VIF08_06845"
     CDS             complement(2879..3832)
                     /locus_tag="VIF08_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8135744.1"
                     /translation="MDNVENVTPNVFTNTKLRGSNPSFVVTSDGVVAIDAPQLPTKAV
                     EWRRETESHGPIRYLINTEHHVDHIFGNYYYRGSGAIVVNHRELWNRFMVPGPEIDPY
                     DYAHEAVPADDPAGEAIFPPRDEYYRDPNKGSIVFTDDITLYVGGHTFNLLFTPGHTQ
                     GQTTVHVPEERVAFTGDTLFSGCQTWLMTSDVDQWITALDRIRTLDVDWLVPGHGPVV
                     TKQYLDVQRAHLLSWKAAVADAVAKGWSREETVARINFKDAFGPVDIGQEYMIDYIQT
                     WNAGTLWDKLTGCPPGGGMPRPLDPRDPDFAARSSALSRRS"
     gene            complement(3854..4630)
                     /locus_tag="VIF08_06850"
     CDS             complement(3854..4630)
                     /locus_tag="VIF08_06850"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8135745.1"
                     /translation="MPTNGLFRLDDRVALIAGGGGAIGSAIGQAFANAGAKVVLVGHS
                     RESTEAAASQIGSAALAVVADATSESECEAAVAQAVERFGRLDICVNAVGGGAGKVLH
                     NAEEYPRADWDWIFELNLRSTVVTTQAAVKQMIASGNGGKVLNLVSVRAFLGINAGYS
                     AYVAAKGAVASLTRQWATEWAKHRINVNAISPTFVDTPQVATLLGDPKFKAGIVSRIP
                     LGRVGTTEDIVGPAIFLCSDAAQFVTGQILGIDGGLTATQ"
     gene            4807..5433
                     /locus_tag="VIF08_06855"
     CDS             4807..5433
                     /locus_tag="VIF08_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135746.1"
                     /translation="MKRSPVALLVAVALVLVVAAPSLAAWAGRGTNMYARTSVYGDCP
                     GSFATWTGPRGGALVLNSECTDPSTSADATLVVSPRCAHDKMNLSCYFSRGGLTALGF
                     DHKVGTYCQGGSPGWDVEAPGGNVWRFACSAGTHTPISSAPGWERITFSAADAQQLAG
                     ATPFVFDGSIKPFFMQVLQDEQGCTTLDNLRINGFKIKKGAAGSHATC"
     gene            5610..6674
                     /gene="hrcA"
                     /locus_tag="VIF08_06860"
     CDS             5610..6674
                     /gene="hrcA"
                     /locus_tag="VIF08_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY8135747.1"
                     /translation="MARRTERPAGPLDPRSQQILRATIEEYVLTAEPVGSQTLVDRYG
                     LGVSSATVRNVMAELEEAGYLTHPHTSAGRVPTDAGYRLYVESIAPRISLAPVERLMI
                     RHQFGQVEFASEQWFRLAAATIAGTTHAAGLATPAKPRSCKLRHIDLVATSTHTASLV
                     VVLAEGGVKQELIPIEDAYDQAELDDVARLLNNELADKSARQVASRMAALAQDPDAPP
                     LAVRVTQRVEKLMREFDAATVEDVFSDGLLNVMSEPEFSQSEKLRRVFAALQNRDYLG
                     SLVARLATAGDVQVLIGGENERAEMRDVSLVLATYGQPGRALGVVGVLGPTRMAYPHA
                     IGTVRYVSGLMNELVEQLYR"
     gene            6671..7312
                     /gene="grpE"
                     /locus_tag="VIF08_06865"
     CDS             6671..7312
                     /gene="grpE"
                     /locus_tag="VIF08_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8135748.1"
                     /translation="MTDFDPRHPQPRLRRTRAEERIAALDTSPAKLTAQIDQLRAELE
                     AAQQEAAENKAAWQRSAADFSNYKRRTEQEREALVGLANEVLLAKLLAIVDDFDRAIA
                     NKPAELADSGWVGGIVAIDRKLDQLLESEGLTPIEALGKQFDPHEHEAIAQEDRPGVP
                     EGTVIAELQKGYRLRDRVLRPAMVAVARSTTTDNQTAAEGGQPETITNSGGLN"
     gene            7315..>8030
                     /locus_tag="VIF08_06870"
     CDS             7315..>8030
                     /locus_tag="VIF08_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8135749.1"
                     /translation="MGRIIGIDLGTTNSVVAVMEGGEPTVIPSAEGGRTVPSVVAFTK
                     NGERLVGQVAKRQAVTNPGNTVYSIKRFMGRRWDDPEVKRSRELVPYKVDKDANSDGI
                     KVTLSDGKGYTPPEISAMILQKLKTDAEAYLGEKVTEAVITVPAYFDDTQRQATKDAG
                     RIAGLDVKRIINEPTASALAYGLDKKKDEQIAVYDLGGGTFDISILELGDGVFEVKAT
                     NGDTHLGGDDFDQRVIDWLLT"
BASE COUNT         1363 a         2667 c         2696 g         1304 t
ORIGIN      
        1 gactcagcgc gtacgacgct gcctaccttc agatggccct cgaggttgag gcggagatcg
       61 cgaccgtcga tcgagatcta gccgctgcgg ccggccgcga agggctcgcc gtcgtctagg
      121 ctcgtgcccg tgactcgcat aatcgatttc gaccggccgc cggagggcgt ggcgcgacag
      181 gacctgccgc ggctgctagg tggaaaagcg gccaacctga caatcatggc ggtcgacttc
      241 gggctgccgg tgccgccggc gttcacgcta acgaccgatg tgtgccgcga gtacctggcg
      301 agcggctggc cgccccgcct cgaggaggag ctgcgcgagc acatggcccg gctcgaggag
      361 ctggtcggcc ggaggttcgg agagccggcg gatccactgc tcgtgtcggt tcgatcggga
      421 gcgccggtat cgatgcccgg gatgatggac acgatcttga acgtcgggct gaacgccgcg
      481 acctctgcgg ccttcgacga gtcgctggcg ccacgcctga aaacccgctt gagcgaatcg
      541 cttgccgcgg tcggcgtcga gcccgttccg gaggaccagt ggggtcagct gcgcgcggcg
      601 atcgaggccg tattccggtc ctggaattcg ccgcgcgcca tcgcctaccg cgagcgcgag
      661 ggcatcagcg ccgatatggg caccgccgcc accgtccagg cgatggtctt cggcaatcgc
      721 aacgagcgat cggcgacagg cgtgctattc acgcgcaacc cgtcaacagg tgagaaccgg
      781 ccgtacggcg acgtgctgtt caacgctcag ggcgaggacg tggtcgcggg cacgcacgcg
      841 acagaggaca tccgcgtctt gcacaagcgg ctgcccgacg tcgcgcgcct gctgcacgag
      901 tacacgcgca cgctcgagcg gcacctcagg gacatggccg acatcgagtt cacgatcgag
      961 gacggcaagc tgtggctgct ccaggtgcgc gtcggcaagc gctcgcccca ggccgcgctg
     1021 cgcctcgccg ccgagatggc ggacgatccc gactttccgc tcagccgcga agaggccgtg
     1081 cgcagagttc tgccgctgct cgcggatccg ccgcgcgtca ccgagaagcc gcgtgacgcc
     1141 gccgagccgc tgaccaaggg acttggcgcg tctccaggtg tcgcggccgg cgaggtcgtc
     1201 accacgcccg aggccgccgt gacggctgcc gagcaggggc gcgccgtgat cctcgttcgc
     1261 gccgagacct cacccgacga cgtccacggc atgtccgtcg cgcgcggcat cctcacggca
     1321 accggcggcc ttgccagtca cgccgcggtt gtcgcccgtg gctggggcat ccccgcagtg
     1381 gttggggctg cggccgtatc ggtcggcagc gagtccatct ccgtgaatgg ccgacagctg
     1441 aaagtcggcg accagatcac catcgatggc tcgacgggtg aggtcttcgg gggcgccctt
     1501 gccggttcgt cggaagttgt cccggaggcc gcgaccttgc ttgattgggc gcgtgagctg
     1561 ggaataccga ttgaaggcac cgattcacct gcttcacctt cggccgagtc ccccggaact
     1621 gcgacgcccg ccgacgcgct gcgactcctg ctggtcaagg gctacgcgac gcccgagcag
     1681 gcggccgagt gcctgatggt ttcgctcgag gacgcgcgct cattgctcgc cggcctagag
     1741 acgagtgccg acgtcgagca ggccgtgggc tcctaccgac tgaccgatgc gggtaaggtt
     1801 cgcgcccgca tggatctcga cgcggcccgc gctgcatggc cggggcccgt cgcggcgctc
     1861 gacgagtttg tcggtctgga caagcaggtc aaggatgtcg ttacgtcgtg gcagatgcgc
     1921 ggcgagtcag tcaacgatca ctcggacgcg gcgtacgacg ccctcgtcct aaacggtctg
     1981 gccgaactgc ataacgagat ccaggcatgg ctcccgtcac gcttcaatcg gtacaggcag
     2041 cgcctggacc gggccgcggc cgcggtcgcg gctggcgaca cgaagttcgt ggcgtcgccg
     2101 cgcgtggaca gctaccactc agcctggttc gaactgcacg aggagctgat ccttcttgcg
     2161 ggccgtactc gtgccgacga ggtggccgcc ggccgcgcct gagtcggtac acgtggtgta
     2221 cacatggtgt atcatcgcgg catgagcaga atccgcacca acatcgagat cgacgacgcc
     2281 gatgtccgca ggatcatggc gcgctattcc ctgaagacca agaccgaggc tgtggccctt
     2341 gctttacgca ccctcgcggg tcagccgatg actctggaag aggcgctcgc tatgcaggga
     2401 tcccatgcaa tcggcgaggt tcctgaggat tgtgggccgc atggaatcga cgaccggccg
     2461 gcgccgcggt gatcctcgcc gacacctcgg catggatcga gtacatccgg gggacggaga
     2521 gtccggttca ccagcgcttg cgtgacctgg ttctgggggc ggaccaactc gtcgtgaccg
     2581 agccagtcct catggaagtt ctgggtggag cgcgcaacga cgacagcgag actgcgctgc
     2641 gtcgcttgct gctgcgatgc gatttcgtgc cgtttctatc ggcgactgac tttgagggcg
     2701 cggccgccgt ctatcgccac tgccgggcgt ccggtgtaac tccgcgtggg tttgtcgact
     2761 gcatgatcgc caccgtcgcg cggagagtcg gcgcgactgt tctggcgcac gacgttgacc
     2821 tcgcgcgaat cgcgtcagtg atcggcttgg agctcgatcc ggcgtccttg gccgcgagct
     2881 aggagcggcg gctcaacgct gaggacctag ccgcgaagtc ggggtcgcgc gggtcgagtg
     2941 gtcgcggcat gccgccgccc ggcggacacc cggtcagctt gtcccagagc gtgcccgcgt
     3001 tccacgtctg gatgtaatcg atcatgtact cctggccgat gtcgaccggc ccgaacgcgt
     3061 ccttgaagtt gatccgcgca acggtctcct cgcgcgacca gcccttggcc acggcatcgg
     3121 ccaccgcggc cttccacgag agcaggtgcg cgcgctggac gtcgaggtac tgcttggtga
     3181 cgacggggcc gtggcccggc acgagccagt caacgtccag cgtccggatg cggtccagcg
     3241 ctgtgatcca ttggtccacg tcgctcgtca tcagccaggt ctggcagcca gaaaacaacg
     3301 tgtcacccgt gaacgcgacg cgctcctccg gcacgtggac ggtcgtctga ccctgggtgt
     3361 gcccgggcgt gaacagcagg ttgaacgtgt ggccgccgac gtacaaggtg atgtcgtcgg
     3421 tgaacacgat cgagcccttg ttggggtcgc ggtagtactc gtcccggggc gggaagatgg
     3481 cctccccagc ggggtcatcg gccgggaccg cctcgtgggc gtagtcgtat ggatcgatct
     3541 caggcccggg caccatgaat cggttccaga gctcgcgatg gttgaccacg atcgcccccg
     3601 acccgcggta gtagtagttg ccgaagatgt ggtcgacgtg gtgctcggtg ttgatcagat
     3661 agcggatggg tccgtgtgac tcggtctcgc ggcgccactc gaccgctttc gtcggcagct
     3721 gcggcgcatc aattgccacg acgccgtccg aggtaacgac gaacgacgga ttcgagccgc
     3781 gcagcttggt gttcgtgaag acgttcggcg tgacgttctc tacgttgtcc acgtgcgtgg
     3841 ctcctagctc ctgctactga gttgcggtga ggccgccatc gatgcccagg atctggccgg
     3901 tgacgaactg cgctgcgtcg gagcagagga agatcgccgg cccgacgatg tcttccgtcg
     3961 tgccgactcg tcccagcggg atgcgcgaga cgatgcccgc cttgaacttg ggatcgccga
     4021 gcaaggttgc cacctggggc gtgtcgacaa aggtcggcga gatggcgttg acattgatgc
     4081 ggtgcttcgc ccactccgtc gcccattggc gcgtcagtga agcgaccgcg cccttcgccg
     4141 cgacgtaggc tgagtacccc gcgttgatgc ccaggaatgc gcgcactgac acaaggttga
     4201 gcaccttgcc accgttgccc gacgcgatca tctgcttcac cgcggcctgg gtcgtgacga
     4261 ccgtcgagcg caggttgagc tcgaagatcc agtcccaatc ggcgcgcggg tactcctccg
     4321 cgttgtgcag caccttgccg gcgccgccgc ccactgcgtt gacgcagatg tccagccgac
     4381 caaaacgctc gactgcctgc gccacggctg cctcgcactc cgactcggag gtggcgtccg
     4441 caacgaccgc cagggcagcg ctgccaatct gggacgcggc cgcttcagtg ctctccctgg
     4501 aatggccgac gagcacgacc ttcgcacctg cattggcgaa ggcctggccg atcgcggatc
     4561 cgatcgcacc gccgccgccg gcgatcagcg cgacgcggtc atcgagtcgg aagaggccat
     4621 tggtgggcac ggctgcagcg taatcgaact ttggctatta gacgctgagg acgttggctc
     4681 atgttggcca gggaccagat ggcactggcg ccgcacgctg ccgccaacta gtctcgaccg
     4741 ggagttcatg gcccggccgg ccgggggagg ccccgggctc gagcacccga gagtcggagg
     4801 aatcggatga aacgttcacc tgtggcgctg cttgtggcgg tcgccctggt tctggtcgtt
     4861 gccgcaccgt cgttggccgc ctgggcgggc agaggcacga acatgtatgc aaggacaagt
     4921 gtctatggcg actgccccgg cagcttcgcg acctggaccg ggccacgcgg cggcgccctg
     4981 gtgctcaaca gcgagtgcac cgatccgtca acgtctgccg acgcgacgct cgttgtctcg
     5041 ccccggtgcg ctcatgacaa aatgaacctc agctgctact tctcgcgggg tggcctcacg
     5101 gcgcttgggt tcgatcacaa ggtcggaacg tattgccagg gcggatcacc gggctgggac
     5161 gtagaagcgc ctggtggcaa cgtttggcgg ttcgcctgct cagccggcac tcacacgccg
     5221 atctcatcgg cgccgggctg ggaacggatc acattctctg cggccgatgc ccagcagctg
     5281 gctggcgcga cgccgttcgt cttcgacggg tcgatcaagc cgttcttcat gcaggtcctg
     5341 caggatgagc agggttgcac gacgctcgac aacctgcgca tcaacggatt caagatcaag
     5401 aagggggcgg ccggcagcca cgcaacctgc tgaggtcctc ggtactgaat gaagaggccc
     5461 gtcccagccg gggcgggcct cttcttctac ccgtgtcgca cagccttcgt tgactcaggc
     5521 gcgccagggg cgtaatattc gttagcactc cggccatgag agtgctaacg actccatcaa
     5581 gcgcggaacc acaggggaaa tcacgccgaa tggcgcgtcg cactgagcgc ccagccggcc
     5641 cgctcgatcc ccgctctcag cagatcctgc gggcaaccat cgaggaatac gtccttaccg
     5701 ccgagccggt cggcagccaa acgctggtcg accgctacgg cctcggcgtg tcttccgcga
     5761 ccgtgcgcaa cgtcatggcc gagctcgagg aagccggcta cctcacgcac ccgcacacca
     5821 gcgccgggcg cgtcccaacg gacgccggct atcggctcta cgtggagagc atcgccccca
     5881 ggatcagcct cgccccggtt gagcggctga tgatccgcca ccagttcggc caggtcgagt
     5941 tcgcgagcga gcagtggttc cgcctcgcgg ccgccacgat cgccggcacg acgcacgcgg
     6001 ccgggctcgc gacgccggcc aagccgcgtt catgcaagct gcgtcacatc gacctcgtgg
     6061 cgacgagcac tcatacggcg agcctcgtcg tcgtcctcgc cgagggcggc gtcaagcagg
     6121 agctcatccc catcgaggac gcctacgacc aggctgagct cgacgatgtc gcgcggcttc
     6181 tcaacaacga attggccgac aaatcggcgc gccaggtggc ctcgcgcatg gccgcgctcg
     6241 cccaagatcc cgacgcgccc ccgctcgctg ttcgcgtcac gcagcgcgtc gagaagctga
     6301 tgcgcgagtt cgacgccgcg accgtcgagg acgtgttctc cgacgggctg ctcaacgtca
     6361 tgtccgagcc cgagttcagt caaagcgaga agctgcgccg cgttttcgcc gcgctccaga
     6421 accgcgacta cctgggctcc ctcgtcgcgc gcctcgcgac cgcgggcgat gtccaggtgc
     6481 tgatcggcgg cgagaacgaa cgcgctgaga tgcgcgacgt ctcgctggtc ctggcgacgt
     6541 acggacaacc cggccgggcg ctgggcgtgg ttggtgtgct gggtcccacg cggatggctt
     6601 acccgcacgc gatcggcacc gtgcgctacg tgagcggcct gatgaacgag ctcgtggagc
     6661 agctgtaccg atgaccgatt ttgacccgcg ccacccgcag ccccgcctgc ggcgcacccg
     6721 tgccgaagag cgcatcgccg cgctcgacac atcgccggcc aagctgaccg cgcagatcga
     6781 ccagcttcgc gcggagctcg aggccgccca gcaggaagcg gccgagaaca aggccgcgtg
     6841 gcagcgctca gcggctgact tcagcaatta caagcgccgc accgagcagg agcgcgaagc
     6901 gctcgtcggc ctcgccaacg aggtgctgct cgccaagctg ctggcgatcg tcgacgactt
     6961 cgatcgggcg attgccaaca agcccgccga gctggccgac agcgggtggg tcggaggcat
     7021 cgttgccatc gatcggaagc tcgatcagct gctcgagagc gagggcctga cacccatcga
     7081 ggcgctgggc aagcagttcg atccgcacga gcacgaggcg atcgcccaag aggaccggcc
     7141 gggcgtccca gaggggaccg ttatcgccga gctccagaag ggctatcgac taagagaccg
     7201 ggtgctccga ccagcaatgg tcgccgttgc ccgatcgaca acaaccgaca accagaccgc
     7261 cgctgaaggc gggcaacccg agaccattac gaattccgga ggactcaact agacatgggc
     7321 aggatcatcg gcatcgacct tggcacgacc aacagcgtcg tggcagtcat ggagggcggc
     7381 gagccgaccg tcatccccag cgccgagggc ggcaggaccg tcccatcggt cgttgcattc
     7441 acgaagaacg gcgagcgcct ggttggccag gtggccaagc gccaggcggt cacgaacccc
     7501 ggcaatactg tctattcgat caagcgcttc atgggtcgtc gctgggacga cccggaggtc
     7561 aagcgctcgc gcgagctcgt gccctacaag gttgacaagg acgcgaatag cgacggcatc
     7621 aaggtgacgc tgagcgacgg caagggctac acgcccccgg agatcagcgc catgatcctc
     7681 cagaagctca agaccgacgc cgaggcgtac ctcggtgaga aggtcacgga ggcggtcatc
     7741 accgtccccg cgtatttcga cgacacgcag cgccaggcga ccaaggacgc gggccgcatc
     7801 gcgggtctcg acgtcaagcg catcatcaac gagcccaccg cctctgccct ggcctacggc
     7861 cttgacaaga agaaggacga gcagatcgcc gtctacgacc tgggcggcgg cacgttcgac
     7921 atctcgatcc tcgagctggg cgacggtgtc ttcgaggtca aggcgaccaa cggtgacacc
     7981 cacctgggcg gcgacgactt cgaccagcgc gtgatcgact ggctgctgac
//
LOCUS       DATBRX010000177         3477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2077690, whole genome shotgun sequence.
ACCESSION   DATBRX010000177 DATBRX010000000
VERSION     DATBRX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077690"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VIF08_06875"
     CDS             <1..214
                     /locus_tag="VIF08_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135750.1"
                     /translation="RGDEVAHGLVEARRAQSRHVPMVVRIVGTNADEAARILAEAGDP
                     DLQTAATLDEAVERAVAAATHAAVAA"
     gene            211..1098
                     /gene="sucD"
                     /locus_tag="VIF08_06880"
     CDS             211..1098
                     /gene="sucD"
                     /locus_tag="VIF08_06880"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY8135751.1"
                     /translation="MSILVGADTRLLVQGITGREGNFHAQQMQDYGTNVVAGMTPGKG
                     GQLALERPVPVFNTCLEAVTETGANTSVIYVPAGGAPDAVLEAVEAGIKTIFCITEHI
                     PALDMLKVVHVVDAAGARLIGPNCPGATSVGTAKVGIIPGSIHRPGHVGVVSRSGTLT
                     YEVVEALTEAGIGQSTCVGIGGDPIAGSNFIDMLALFRDDPETNAVVLIGEIGGDGEE
                     RAAEWIAANMNGAPVASFIAGRTAPPGRRMGHAGAIISGGRGTAQSKVAALEAAGVRV
                     ADAPSQIPQLLLEMGVKPS"
     gene            1145..2113
                     /locus_tag="VIF08_06885"
     CDS             1145..2113
                     /locus_tag="VIF08_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135752.1"
                     /translation="MSQFTGGTIGNYSRLWLIITGAILLGVGAILAIMLGNIPFAGGA
                     MVMTGGFMALVGIVLIVIGIIVGRRAAATDALLQTGTPGTATITGLTQTGMYVNENPQ
                     IRMQLLVALPGQTPYATQHTEIVPLMLLGRLNSGAPLQVRVDPLNLNRIAVDWGASGL
                     GGGSPVMGGIGAVPMGGVGAVPMGTAGATSAAGATSGSGMDESLAQVQAAMAATGMAA
                     AAPFQTAAQGNYTVEQLRAYLRASGQDASATIDKLEDSGKVVGDEHLFTMEMTLKIPG
                     QPDKKLPASAAMVPVAKAHRLFQGMTVPVKYEATNPNLLMVDWEKV"
     gene            complement(2378..2845)
                     /gene="mce"
                     /locus_tag="VIF08_06890"
     CDS             complement(2378..2845)
                     /gene="mce"
                     /locus_tag="VIF08_06890"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY8135753.1"
                     /translation="MSEPERTFDISKHPGRVHHVAVVVRDLDAALAFYRDTLRLELDV
                     VMPIESDGVKIGFLPVGESSIELVQPTRDDTGVARFLANKGEGFHHVCLEVDDLAAEL
                     SRLAGEGIELIDTAPRRGAEGPVAFIHPRSCHGVLVELIEAPGGPAWESLGYH"
     gene            complement(2842..>3477)
                     /locus_tag="VIF08_06895"
     CDS             complement(2842..>3477)
                     /locus_tag="VIF08_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8135754.1"
                     /translation="TTIEALAAVLGGAQSLHTNARDEALALPTEAAARLALRTQQVIA
                     YESGVTATPDPLAGSYFIERLTDQLEAAANDYLAEIDALGGAVKAIEAGYQQRQIQEA
                     AYHSQRRIEAGEQIVVGVNKFMDDEVHTPDLQRIDPQGERDQVARVARVRAERDAGAF
                     EASLRALAETARGDGNLVPAIIDAVKARATLGEISDALRVAFGEHRELITV"
BASE COUNT          552 a         1209 c         1194 g          522 t
ORIGIN      
        1 ccgcggcgac gaggtggcgc acggcctggt cgaggcacgc cgcgcgcagt cgcgccacgt
       61 gccgatggtt gtgcgcatcg tcggcacgaa cgccgacgag gcagcgcgca tcctcgccga
      121 ggccggcgac cccgacctgc agacagccgc gaccctcgac gaagcagttg agcgcgcggt
      181 cgcggcggcg acgcacgcag cggtggcggc atgagcatcc tggtcggcgc cgacacgcgc
      241 ctcctcgtcc agggcatcac cggccgtgag ggcaacttcc acgctcagca gatgcaggac
      301 tacggcacga acgtggttgc gggcatgact cccgggaagg gcggccagct ggcgctcgaa
      361 cggcccgtgc ccgtgttcaa cacgtgcctc gaggcggtca ctgagaccgg cgccaacacg
      421 agcgtcatat acgtcccggc cggcggcgcg ccggacgctg tgctggaagc ggtcgaggcg
      481 ggcatcaaga ccatcttctg catcaccgag cacatccctg ccctcgacat gctcaaggtc
      541 gtgcacgtgg tcgatgcggc tggcgcccga cttatcggcc ccaactgccc cggcgcgacg
      601 tccgtcggca cggccaaggt gggcatcatc ccgggctcga ttcaccggcc cggccatgtc
      661 ggcgtcgtca gccgctcggg taccctgacc tacgaggtcg tggaggcgct gacggaagcg
      721 ggcatcggcc agtcgacgtg cgtgggcatc ggcggcgacc cgatcgcggg ctcaaacttt
      781 atcgacatgc tggcgctctt ccgcgacgac ccggagacga acgcggtcgt gctgatcggc
      841 gagatcggcg gcgacggcga ggagcgcgcc gcggaatgga tcgcggccaa catgaatggt
      901 gcgccggtgg cgtcgttcat cgccggccgg acggcaccgc ccgggcgacg catgggtcat
      961 gccggcgcga tcatcagtgg cggtcgcggg accgcgcaga gcaaagtggc tgccctggag
     1021 gccgcgggcg tccgtgtcgc ggacgcgccc agccagatcc cgcagctgct cctggagatg
     1081 ggcgtcaagc cgagctgatc ggcggacgtc acattcggcg gcaagccaga aggagaggaa
     1141 caccgtgtcg cagttcaccg gggggacaat cggcaactac agtcggctct ggctgatcat
     1201 caccggcgcc atcctgctgg gtgtcggcgc gatcctggcg atcatgctcg gcaacatccc
     1261 gttcgccggc ggagcgatgg tgatgaccgg cggcttcatg gccctcgtcg gcatcgtgct
     1321 gatcgtgatc ggcatcatcg tcggccgccg cgcggcagcg acggacgcgc tgctccagac
     1381 gggcacgccg ggcacggcga cgatcaccgg gctgacgcag accggcatgt acgtcaacga
     1441 gaacccacag atccggatgc agttgctcgt ggcgctgccc ggccagactc cgtacgccac
     1501 gcagcacacg gagatcgtgc cgctgatgct gctcggccgg ctcaactcgg gcgcgccgct
     1561 gcaggtgcgc gtcgatcccc tgaacctcaa tcgcatcgcg gtcgactggg gcgccagcgg
     1621 cctcggtggt ggctcgcctg tcatgggcgg catcggcgca gtacccatgg gtggcgttgg
     1681 cgcagtaccc atgggcacag ctggcgccac gagtgcggcc ggcgcaacca gcgggtccgg
     1741 catggacgag agcctcgccc aggtccaggc cgcgatggcc gcgaccggca tggccgccgc
     1801 agcgcccttc cagaccgcag cccagggcaa ttacaccgtc gaacaactgc gcgcctacct
     1861 gcgcgccagc ggtcaggacg cgagcgcgac gatcgacaag ctcgaggact cgggcaaggt
     1921 cgtcggcgac gagcacctct tcacgatgga gatgacgctc aagatccccg gccagcccga
     1981 caagaagctg ccggcatcgg ccgccatggt gccggttgcc aaggcgcaca ggctcttcca
     2041 gggcatgacc gttccggtca agtacgaggc cacgaacccc aacctgctga tggtcgactg
     2101 ggagaaggtc tagccctcat gccgacccat cgcccggccc agagcgccgc cgccgccggg
     2161 tctcatccca gcatttgtag cggcctgtcg accataagcc acccatcgcg gcgtccgcgg
     2221 gttaccggcg tgcaaatcca cgctcagcga tgacaagacg cgggcgattt cggtcccgac
     2281 gtacctgatc ggtggcttaa ggctgaccta tgcgcacatt tgctgggatg tttgggccgc
     2341 tcagcccgga gcgaccgcac ggcctgcgac cgaccgatca gtggtagccg agggactccc
     2401 aggccggccc gccgggggcc tcgatcagct cgaccagcac gccgtggcat gaacgcgggt
     2461 ggatgaacgc caccggtcct tcggcgccac gccgcggagc ggtgtcgatc agctcgatcc
     2521 cctctccggc caggcgactc agctcagcgg ctagatcatc gacctccagg cacacgtggt
     2581 ggaagccctc gcccttgttg gcgagaaagc gcgcgacgcc cgtgtcgtcc cgcgtcggct
     2641 ggacgagctc gatgctcgac tcgccgacgg gcaggaagcc gatcttgacg ccgtcgctct
     2701 cgatgggcat gaccacgtcg agttccaggc gcagcgtgtc tctgtagaag gcgagcgctg
     2761 cgtccagatc gcgcacgaca acggccacgt gatgcacgcg tccggggtgc ttactgatat
     2821 cgaacgtgcg ttctggttcg ctcataccgt gatcagctct cggtgctcgc caaaggccac
     2881 gcgcaacgcg tcactgatct cgcccagcgt cgcgcgcgcc ttgaccgcgt cgatgatcgc
     2941 ggggacgagg ttgccgtcgc cgcgcgcggt ctcagccaac gcgcgtaaag acgcctcgaa
     3001 cgccccagcg tcgcgctccg cgcggacgcg tgccacccgc gcgacctggt cgcgctcccc
     3061 ttgcgggtcg atgcgctgca ggtcgggcgt gtggacctcg tcatccatga acttgttgac
     3121 gccgaccacg atctgctcgc ccgcctcgat ccgtctttgc gagtgatagg ccgcttcctg
     3181 gatctggcgc tgctgatagc cggcctcgat cgccttgacc gcgccgccga gcgcgtcgat
     3241 ctcggcgagg tagtcgttcg ccgccgcctc gagctggtcg gtcaggcgct cgatgaagta
     3301 cgagccggcg agcgggtcgg gcgtggccgt cacgccgctc tcgtacgcga tgacctgctg
     3361 agtccgcagc gcgaggcgcg cggccgcttc cgtgggcagg gcgagcgcct cgtcgcgcgc
     3421 gttcgtgtgg agcgactgcg cgccacccag gaccgcggcg agcgcctcga tcgttgt
//
LOCUS       DATBRX010000178         8928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1744126, whole genome shotgun sequence.
ACCESSION   DATBRX010000178 DATBRX010000000
VERSION     DATBRX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744126"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1795)
                     /gene="argS"
                     /locus_tag="VIF08_06900"
     CDS             complement(137..1795)
                     /gene="argS"
                     /locus_tag="VIF08_06900"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY8135755.1"
                     /translation="MSTTRDLVRDRVVAAFVAAVEDGRLPPFPPEPPVVFDVTRPAST
                     EHGDYATNAALKMAGKMGRPPRELATVLAAAIGTDDVIAKVDVAGPGFVNIFLAPGYV
                     ERAIDEIRAAGTSYGRGQLSAPKKINVEFVSANPTGPLHVGNARGAFVGDLLCRVLEA
                     AGVDVTREYYFNDIGGQVERLGLSVAALKQGQPVPDDGYHGDYVGDLARDLPDDVWQR
                     AQSEPERMGWIVGEWASEATRGGIERSLENLGVHIDVWKGEGSLHAEGYVSRAIEKLR
                     ASGDIYEQDGAHWFRSTKYGDDKDRVVIRSNGEPTYFASDIGYVSEKFSRGFDELIYI
                     WGADHHGTIARLKNAAQALGFDRDATNVLLIAWVHFVRDGVEVSMSKRTGEFVTLDEL
                     LAEVGVDAARWFFASRAPSTGIDFDIELAKKESSENPVYYVQYAHARISSILRKATDE
                     GLKTADSLAGGLAADPVALGLAKDLLRLPDIVRDAAEERETQSITAYATELATTFHAF
                     YRDRRVVDASDPVTSGYRLALVDATRVTLAAALGLLGISAPDSM"
     gene            complement(1806..2567)
                     /locus_tag="VIF08_06905"
     CDS             complement(1806..2567)
                     /locus_tag="VIF08_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8135756.1"
                     /translation="MKLTVVGCAPAYTARTGRASSCYLVEHGPTRLALDFGLGAFAEL
                     WRYTPPGELTAIAISHLHADHNVDLIPLRHWVRFENDGRGPALHAPADLRQRMADYQQ
                     FPDPHTKPSFLADFAGDPLAVGSFVVGDLLIEAKHVTHIADSFAFRVSVAGGDGPGLV
                     YSGDCAVADDLLPLIRPGDTLLCEAAFGSGPRDAPIHLTAAEAAGAALRGDASRLVLT
                     HILDKRDEAGSVAIAGGVFGGAVEAAQPGLTLDIS"
     gene            complement(2564..3202)
                     /locus_tag="VIF08_06910"
     CDS             complement(2564..3202)
                     /locus_tag="VIF08_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY8135757.1"
                     /translation="MAQRWVCLDVGETLIDESRVWETWAQILGVTRFTFMAAMGAMLA
                     RGRQFDEVFEFLGVKNRDEHRAAFNAAYGGFQERDLYPDAKPALDALRAAGYRVAIIA
                     NQPAERTDELRFLGVRAEVMGMSAEMGVHKPAPEFYVAALQRMGDPPAAEVAYVGDRL
                     DNDIDPAAAAGMRTVWIRRGPWAAITTDTPPPGTLVVDSLDELVRRIDEVWA"
     gene            complement(3216..4451)
                     /locus_tag="VIF08_06915"
     CDS             complement(3216..4451)
                     /locus_tag="VIF08_06915"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEY8135758.1"
                     /translation="MATTMEPTTPITRTDPLAYLQAEIDDLRAKNLYRPLRIMSGAQA
                     VHTTVMGRPVISLSSNNYLGLTTHPRLVEAAIKAVRELGVGTGAVRTIAGATELIEEL
                     ERRLASFKHVEAVLTLQSGLTSNSGTIPAITAEGDLIVSDELNHASIIDGVRLSRAER
                     TVFPHRDVDGLEKVLREARANGGKNGPYKNILVITDGVFSMDGDIAPLAGICDVADRY
                     EAAVMVDDAHASGVLGKNGRGTIDHFDLHGRVDIQVGTLSKAVGVMGGYIAGRQHLKD
                     FLTQRCRPLLFSTSQPPAVAAACIAAIDVLEGEPERIERLWDNARFFKSALQGLGFDT
                     GISETPITPVIAGDEAKAQQLAARLFEEGVFATSVVFPTVALGKARVRTIVTSEHSRD
                     DLHTCINAFEKVGRELRLI"
     gene            complement(4457..5539)
                     /gene="tdh"
                     /locus_tag="VIF08_06920"
     CDS             complement(4457..5539)
                     /gene="tdh"
                     /locus_tag="VIF08_06920"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEY8135759.1"
                     /translation="MTTQAAAVIDAPPRGIAMRALVKESAGPGARVREVPVPEPGPGE
                     ILVKVLAASVCGTDVHIERWDKWAQDNFGPPPMTFGHEMAGIVVARGPGAMRIPLGEL
                     IAAETHWVDWTCYQCRTGRAHVCQNNRTLGVKVPGAFAQYVVMPEANAWVSDGLTPEI
                     AALQEPLGNAVHAAFVEEVAGQTAVVTGCGPIGLMSIGVLKMAGARAVYATDINPERL
                     AMATQMGADLALDARDDVVGRLRAETDGNGVDIVLEMSGSAAALHQGLAAVTNGGRVS
                     LLGTHATPATLDLSQEVIFKGIRVYGITGRRLWDTWYRTTALLEEGLDISPIITHRLP
                     LGDYAQAFDLVASGHAGKIVLLPQEE"
     gene            complement(5591..6478)
                     /locus_tag="VIF08_06925"
     CDS             complement(5591..6478)
                     /locus_tag="VIF08_06925"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HEY8135760.1"
                     /translation="MASGSGSTRPRTSRPRRAYAQPSLFERYRSLIFGGAAVFGVVIL
                     AWLFLQGGARAGYECGALLTPGPSESLTPGLPTSTPSPTPSATPTPSITAGPSGSAGP
                     SGSPTAAPTGSPTAAPSPTPVATPVPEPTPRLGFSTGLLGRNHVRDLNTVIKYTFCPP
                     TSGDHFNDLSGGRGPIRPAVYPPNEEQIPGGWLHNMEHGYVVALYRCPSGVLGEGDCI
                     SQAEMDEMQAFFDQVPDSINPSCPTKVLVARFDSMDTKFAVLAWGRALLTNEFDLDTA
                     LLFNQQWQDQPAAPERGSC"
     gene            complement(6678..7049)
                     /locus_tag="VIF08_06930"
     CDS             complement(6678..7049)
                     /locus_tag="VIF08_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135761.1"
                     /translation="MFEVVRAMPPGVRVVLAFGFLLLAGLGLTLPLVVREAVEAPVSP
                     LGLLYMLLLAYLVFTLTLILQRKQAAWMLSLGLASLSVPLAIGLYSLAGTLGLAAGIL
                     LTVVLFWSLRGERVRGWFNEP"
     gene            7270..8394
                     /locus_tag="VIF08_06935"
     CDS             7270..8394
                     /locus_tag="VIF08_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8135762.1"
                     /translation="MEERVSERVSDRSSDPPAPRARIADVAREAGVSKAAVSFAFNSP
                     ERLKPQTAERIRQVALTLGYRPHPVARMLTAGSTATLGILSPNPLAQAFANPFFALFA
                     EGVSAVTEDRGFGLLFISPLHGSLERAMERATVDGVIVIGLDHHHPEVEAIRRTGVPT
                     VLVDAPAWPEHGAIAVDDTAGALAAATHLLELGHRDLLVLTMEPSESQDVDAAGSEGG
                     SVMSRRMRGYRLALEDYEVKLGAGSIRQAPATFEGGEAAFLRAWEDGLRPTGVLSMSD
                     AAAAGVLQAARHLGLSVPNDLSIVGFDDLPLTRFTDPPLTTVHQPVRRKGEEAARMLL
                     RALSPGSGRVGEHRVLETRLVVRRSTAPPRQPSQEVIGDG"
     gene            8512..>8928
                     /locus_tag="VIF08_06940"
     CDS             8512..>8928
                     /locus_tag="VIF08_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135763.1"
                     /translation="MSRTLNMGGALTALVLILAACAGPGASPTPAATPSRTATPSAAP
                     ATPTAAPSATAEASPTAEASATPNVTPSPTPPPGAVEGTLTIWADNTRAPILANVAAA
                     FTAEYNVPVQIYEIGFGDIRDQLVLRGPANEGPDIII"
BASE COUNT         1420 a         2966 c         3156 g         1386 t
ORIGIN      
        1 cgcggacgca atggagttca agtcgcgcat cgatgagctc tccggcacgt tcaccggcgt
       61 gacggtgacc ggcatcaact tcacgcgcgt cgacgtaagc atcgccgacg aatagccgcg
      121 gcctccttgc ggagagctac atcgagtcgg gcgcggagat ccccaacagc ccgagcgcgg
      181 cggccagggt gacgcgcgtc gcatcaacca gcgcgaggcg gtagccggat gtcaccggat
      241 cggacgcgtc aaccacgcgc cggtcgcggt agaacgcgtg gaaggtcgtg gcgagctcgg
      301 tcgcgtaagc ggtgatcgac tgcgtctcgc gctcctcggc cgcgtcacgg acgatgtccg
      361 gtaggcgcag cagatccttg gccaggccga gcgccacggg gtcggcggcc agaccgccag
      421 cgagtgaatc ggccgtcttc aagccctcgt ccgtggcctt gcgcaggatg gagctgatgc
      481 gcgcgtgcgc gtactgcacg tagtagaccg ggttctcgct cgactccttc ttggccagct
      541 cgatgtcgaa gtcgatgccg gtgctcggcg cgcgcgaagc gaagaaccag cgcgcggcgt
      601 caacgcccac ctctgcgagc agctcgtcga gcgtcacgaa ctcgccggtg cgcttgctca
      661 tcgagacctc gacgccgtcg cgcacgaagt ggacccacgc gatgagcagc acgttggtcg
      721 catcgcgatc gaaacccagc gcctgggctg cgttcttgag ccgcgcgatg gtgccgtggt
      781 gatccgcgcc ccagatgtag atgagctcgt cgaagccgcg gctgaacttc tcgctgacgt
      841 agccgatgtc gctcgcgaag taggtcggct cgccgttcga gcggatgacg acacgatcct
      901 tgtcgtcgcc gtacttcgta gagcggaacc agtgcgcgcc gtcctgctcg tagatgtcgc
      961 cgctggccct gagcttctcg atcgcgcggc tgacgtagcc ctcggcgtgg agcgagccct
     1021 cgcccttcca gacatcgatg tgcacgccca ggttctcgag gctgcgctcg atgccgccgc
     1081 gcgtcgcctc tgacgcccac tcacccacga tccagcccat gcgctcaggc tccgattggg
     1141 cgcgctgcca gacgtcatcg ggcaggtcgc gggcaagatc accgacgtag tcgccgtggt
     1201 agccgtcgtc gggcacgggc tggccctgct tcagggccgc gaccgacaag cccagccgct
     1261 cgacctggcc gccgatgtcg ttgaagtagt actcgcgcgt gacatcgacg ccggcggcct
     1321 ccagcacgcg gcacagcagg tcgcccacga acgcgccgcg cgcgttaccc acgtggagcg
     1381 ggccggtggg gttggcgcta acgaactcga cgttgatctt cttcggcgcg gatagctggc
     1441 cgcggccgta cgacgtgccg gcagcgcgga tctcgtcgat ggcgcgctcg acgtagcccg
     1501 gcgccaggaa gatgttgacg aagcccggcc cggcgacgtc aaccttggcg atgacgtcgt
     1561 cggtgccgat ggccgccgcg agcaccgtcg cgagctcgcg cggtggccgg cccatcttgc
     1621 cggccatctt gagcgcggca ttggtcgcgt aatcgccgtg ctccgtgctc gcggggcgag
     1681 tgacatcgaa gacgacaggc ggctccggcg ggaagggcgg caggcgtccg tcttcaacgg
     1741 ccgcgacaaa cgcggccaca acccggtcgc gaaccaggtc gcgcgtcgtg ctcatgagcg
     1801 cgagcctaac tgatgtcgag cgtgagcccg ggctgcgccg cctcgaccgc gccgccgaag
     1861 acccctccgg cgatcgccac cgatccggcc tcgtcgcgct tgtcgaggat gtgcgtcaga
     1921 acgagacgcg aggcgtcgcc gcgcagcgcc gcgcctgcgg cttcggccgc ggtcaggtga
     1981 atgggcgcgt cgcgggggcc gctgccgaaa gccgcctcgc acagcagcgt gtcaccagga
     2041 cgtatcagcg gcagcaggtc gtccgcgacc gcgcagtcac ccgagtagac caaccccggc
     2101 ccatcaccac ccgcgaccga cacgcggaac gcgaaagagt cggcgatgtg cgtgacgtgc
     2161 ttcgcctcga tgagcaggtc gccgacgacg aagcttccga ccgcgagcgg gtcgccggcg
     2221 aagtcggcca ggaatgacgg cttcgtgtgt gggtccggga actgctggta gtcggccatg
     2281 cgctgtcgca ggtcggcggg tgcgtgaagc gcagggccgc gaccgtcgtt ctcgaacctg
     2341 acccagtggc ggagcgggat gagatcgacg ttgtggtcgg cgtgcaggtg gctgatggca
     2401 atcgcggtca gttcgcctgg cggcgtgtag cgccacagct cggcgaacgc gcccaggccg
     2461 aagtcgagag caaggcgtgt cgggccatgc tcgacgaggt agcaagatga ggcgcggccg
     2521 gtccgcgcgg tgtaggccgg cgcgcagccg accacggtca gcttcacgcc cagacctcgt
     2581 cgatgcgccg cacgagctcg tcgagcgaat ccacgacgag cgttccaggt ggtggcgtgt
     2641 ccgtcgtgat ggccgcccag ggtccgcgcc ggatccagac cgtccgcatc ccggctgcgg
     2701 ccgcgggatc gatgtcgttg tcgagccgat cgccaacgta tgcgacttcg gccgcgggcg
     2761 gatcgcccat gcgctgtagc gccgccacgt agaactcggg tgccggcttg tgcacgccca
     2821 tctcggcgct catacccatg acctcggcac ggacgcccag gaagcgcagc tcgtcggttc
     2881 gctctgcagg ctggttggca atgatggcga cgcggtaacc ggcggcgcgc agcgcatcga
     2941 gcgcgggctt cgcgtcaggg tagagatcgc gttcctggaa cccgccgtac gccgcgttga
     3001 aggcggcacg atgctcgtcg cggttcttga cgcctaggaa ctcgaatacc tcatcgaact
     3061 ggcggccgcg cgcgagcatc gcgcccatcg ccgccatgaa ggtgaagcgc gtcacgccca
     3121 ggatctgcgc ccatgtttcc cagacgcgcg actcgtcgat cagcgtctca ccgacgtcca
     3181 ggcaaaccca gcgctgcgcc acgattagag gggcatcaaa tcaggcgaag ttcgcggcct
     3241 accttctcga aggcgttaat gcaggtgtgg agatcgtcgc gcgagtgctc gctggtgacg
     3301 atcgtgcgca cgcgcgcctt gcccagcgcg accgtcggga agacgacgct cgtggcaaag
     3361 acgccctcct cgaagagccg cgcggcgagc tgctgcgcct tggcctcgtc gccggcgatg
     3421 accggagtga tgggcgtctc ggagattccg gtgtcgaagc cgaggccctg gagggccgac
     3481 ttgaagaagc gggcgttgtc ccacaggcgc tcgatccgct ccggctcccc ctcgagcacg
     3541 tcgatcgccg cgatgcatgc cgccgcgacg gccggcggtt gcgacgtgga gaagagcagc
     3601 gggcgacagc gctgggtcag gaagtccttg aggtgttgcc gcccggcgat gtagccgccc
     3661 atgacgccga cggccttgga gagagtgccg acctggatgt cgactcggcc gtggaggtcg
     3721 aagtgatcga tggtgccgcg gccgttcttg ccgaggacgc cgctcgcgtg ggcgtcgtcg
     3781 accatgactg ccgcctcgta cctatccgcc acgtcgcaga tcccggcgag cggcgcgata
     3841 tcgccgtcca tcgagaagac gccgtcggtg atgaccagga tgttcttgta cggcccgttt
     3901 ttgccgccat tggcgcgcgc ctcgcgcagg accttctcca ggccgtcaac gtcgcgatgc
     3961 gggaagaccg tgcgctcggc gcgcgacagg cgcacgccgt cgatgatcga ggcgtggttg
     4021 agctcgtcgc tgacgatgag gtcgccctca gcggtgatcg ccgggatcgt gccgctgttc
     4081 gacgtcaggc cgctctgcag ggtcaggacc gcctcgacgt gcttgaagct cgccaggcgt
     4141 cgctcgagct cctcgatcag ctcggtcgcg ccggcgatgg tgcgcacggc gccggtgccg
     4201 acgcccaatt cgcgcacggc tttgatggcc gcttcgacga gccgcggatg ggtggtcagg
     4261 ccgaggtagt tgttcgacga gaggctgatg accggccgac ccatgaccgt ggtgtgcacg
     4321 gcttgggcgc cggacatgat gcgcagcggc cggtacaggt tcttggcccg caggtcgtcg
     4381 atctctgcct ggagatacgc cagcgggtca gtgcgggtaa tcggggtagt cggctccatc
     4441 gtggtcgcca tttcgtctac tcctcctgcg gcaagaggac gatcttgcct gcatggccag
     4501 acgcgaccag gtcgaaggcc tgtgcatagt cgccgagtgg caggcggtgg gtgatgatcg
     4561 gtgagatgtc gaggccttcc tcgagcagcg ccgtcgtgcg ataccacgtg tcccacaggc
     4621 gccggccggt gatgccgtag acgcggatgc ccttgaagat gacctcctgg ctgaggtcga
     4681 gcgtcgcggg cgtggcatgc gtgccaagca gtgacacgcg gccgccattt gtgactgcgg
     4741 ccaggccctg gtggagcgca gcggcagagc cgctcatctc gagcacgatg tcgacgccgt
     4801 tgccgtccgt ctctgccctc aagcggccga ccacgtcatc acgtgcgtcg agggcaaggt
     4861 cggcgcccat ctgcgtcgcc atcgccaggc gctcggggtt gatgtcagtg gcgtagacgg
     4921 cgcgcgcgcc ggccatcttg agcacgccaa tgctcatcag gccgatcggc ccgcagccag
     4981 tgactacggc cgtctggccg gcgacctcct cgacgaacgc ggcatgcacg gcgttgccga
     5041 gcggctcctg gagcgccgcg atctcaggcg tcaggccgtc cgacacccac gcattggcct
     5101 caggcatgac gacgtattgg gcgaatgcgc cgggcacctt gacgccaagg gtgcggttgt
     5161 tctggcagac gtgggcgcgg cccgtacggc actggtagca cgtccaatcg acccagtgcg
     5221 tctcagccgc gatgagctcg cccaagggga tgcgcatcgc gcccgggccg cgcgcgacga
     5281 cgatgcccgc catctcgtgg ccgaaggtca tcggcggcgg accgaagttg tcctgcgccc
     5341 atttgtccca gcgctcgatg tgtacgtcgg tgccgcacac gctggcggcc aggaccttga
     5401 cgaggatctc cccgggcccc ggctcaggca ccgggacctc gcgcacccgc gcgccgggac
     5461 cggcagattc cttgaccagg gcgcgcatgg cgatccctcg cggcggagcg tcgatgacgg
     5521 ccgccgcttg ggtggtcatg cggtagtggt agtgctccgg cggctgacgc gcatagtgct
     5581 ggatgtcatc tcaacaactg cctcgctcgg gtgccgctgg ctggtcctgc cattgttggt
     5641 tgaaaagcag cgccgtgtcg agatcaaatt cgtttgtgag cagggcgcgg ccccaggcga
     5701 gcaccgcgaa cttcgtatcc atgctgtcga aacgggcgac gaggaccttg gtcgggcagg
     5761 acggattaat cgaatccggt acctggtcga agaaggcctg catctcgtcc atctcagcct
     5821 ggctgatgca gtcaccctcg ccgaggacgc cgctcgggca gcgatacagc gcgaccacgt
     5881 agccgtgctc catgttgtgc agccacccgc ccggaatctg ctcctcgttg ggcgggtaga
     5941 cggcaggcct gatggggccc cggcctcctg agaggtcgtt gaagtggtcg cccgacgttg
     6001 gcgggcagaa ggtgtacttg atgaccgtgt tcaggtcgcg cacatggttg cgaccgagca
     6061 gcccggtgct gaagcccagg cgcggcgtcg gctccggcac cggcgtggcc accggcgtcg
     6121 gtgagggcgc ggctgtcggc gaaccagtgg gcgcggcggt cggtgagccg gatgggccgg
     6181 cagagccgct gggcccggcc gtgatcgagg gtgtgggcgt ggccgatggc gttgggctgg
     6241 gcgttgacgt gggcaggccg ggcgtaagcg actcgctcgg gccgggcgtc aacagcgcgc
     6301 cgcactcgta accggcgcgt gccccaccct gcaggaacag ccaggcgagg atcacgacgc
     6361 cgaacacggc tgcgccacca aagatcagag agcggtagcg ctcgaacagg ctgggttgcg
     6421 cgtacgcgcg gcgcgggcgc gaggtgcggg gacgcgtgct gccggagcca ctcgccacgc
     6481 gagtctggtc gcccgaagtg ggagcggcgg ttggctcggg acgcgtcgga cgacgtgatc
     6541 gcttggccac ttgtgaacgg tctccgttag gtcgtgacgc cgcgccggcg ttcgcacggg
     6601 cggagcagag ctagctgaat tggcgcaagc gtacgggaat cgccgcttac gcgcctgtca
     6661 ccgaccggca acggggatca cggttcgttg aaccagccgc gcacccgttc accgcgcagc
     6721 gaccagaaga gcacgacagt caggaggatg cccgcggcca aaccgagtgt gccggccagg
     6781 gagtacaggc cgatcgccag cggcacgctg agcgacgcga ggcccaggct gagcatccac
     6841 gccgcctgct tgcgctgcag gatgagcgtg agggtgaaga cgaggtacgc cagcaggagc
     6901 atgtagagca ggccgagtgg cgagacgggc gcctccaccg cctccctgac gaccagcggc
     6961 agcgtcagcc cgaggcccgc caggagcagg aagccgaagg caagaacgac gcgcacgcct
     7021 ggcggcatgg cccgcacgac ttcgaacacc cgctcattct cgcatcggtc gaagtgacgt
     7081 cggactcccg ggacgggtaa tcgggcggat ttggacttga caccgcccag ccagtgttgg
     7141 atggttgcta aaccggtttg ctaaaccggt tgaggagagc gcttgcgagg cccggacagg
     7201 ctcggcggcc gtccggtcca ccaggtgcag accgcgacaa taggccgcgg ataggcggag
     7261 tagcggcggt tggaagagcg tgtgtcggag cgtgtgtcgg accgttcatc ggatccgccg
     7321 gcacccaggg cacgaatagc cgatgtcgca cgcgaggcgg gcgtcagcaa ggcggctgtc
     7381 tcattcgcgt tcaacagtcc cgagcggctc aagccgcaga cggccgagcg catccgccag
     7441 gtcgcgctga cactcggcta ccggccgcac cccgtcgcgc gcatgctgac cgccggctcg
     7501 acggcaacac tgggcatcct gtcacccaac ccgctcgcgc aggcatttgc caacccgttc
     7561 ttcgcccttt ttgccgaggg cgtgtcggcg gtgaccgagg accgtggctt cgggctgctc
     7621 ttcatctcgc ccttgcacgg ctcgctggag cgggcgatgg agcgcgcgac cgttgacggc
     7681 gtgatcgtga tcggcctgga ccaccaccat cccgaggtcg aggccatccg gcgcactggc
     7741 gtgccgaccg tcctcgtgga cgcgcccgca tggccggagc acggcgcgat cgccgtcgat
     7801 gacacggccg gcgcgctggc ggccgcgacg cacctgctcg agctgggcca ccgcgaccta
     7861 ctggtgctga cgatggagcc gtccgagtcg caggacgtcg acgcggcggg tagcgaaggc
     7921 ggcagcgtga tgagccgccg catgcggggc tatcggctcg ccctggagga ctacgaggtg
     7981 aagctgggcg cgggcagcat tcgccaggcg ccggccacct tcgaaggtgg cgaagccgcc
     8041 ttcctgcgcg cctgggaaga tgggctgcgg cccacgggcg tgctttcgat gagtgacgct
     8101 gcggctgcag gagtcctgca ggccgcccgt cacctgggcc tatcggtacc gaacgatctg
     8161 agcatcgtcg gcttcgatga cctgccactc actcgcttca ccgatccgcc actcacgacc
     8221 gtccaccaac cggtgcgacg caagggggag gaggcggcgc ggatgttgct gcgggcgttg
     8281 agcccgggca gtggccgcgt gggtgaacac cgtgtcctgg agacgcgcct ggttgtgcgc
     8341 cgctcgacgg caccgcccag gcaaccgagt caggaggtga tcggcgacgg ctgatgcgat
     8401 gagtggaatt gcggcgtagg ccgcgcgacc ggcgcacaga tcgtgcgcat cggctatcaa
     8461 tgcataatcg gtcgccagcc atggcggccg gcaaggagga ttaaccagag gatgtctcga
     8521 actctcaaca tgggcggcgc ccttacggcg ctcgtcctga tcctggcggc ctgtgcgggc
     8581 ccgggagcgt cgccgacacc ggcggccacc ccctcgcgca cggccacacc gagcgcggcc
     8641 ccggccacgc caaccgcggc gccttccgcg accgcggagg cgagcccgac ggcagaagcg
     8701 tccgcaacgc ctaacgtcac gccctcaccc acgccgcctc cgggcgccgt cgaaggcaca
     8761 ctgacgatct gggctgacaa cacccgcgcg ccgatcctcg ccaacgtggc ggcggcgttc
     8821 acggcggagt acaacgtgcc ggtgcagatc tacgagatcg gcttcggcga tatccgcgac
     8881 cagctcgtgc ttcgcggccc ggccaacgag ggcccggaca tcatcatc
//
LOCUS       DATBRX010000179        11902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1074193, whole genome shotgun sequence.
ACCESSION   DATBRX010000179 DATBRX010000000
VERSION     DATBRX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074193"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(424..1041)
                     /locus_tag="VIF08_06945"
     CDS             complement(424..1041)
                     /locus_tag="VIF08_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY8135764.1"
                     /translation="MSLRLIPRDIKFFDLFEAGGENVAAAGKALHELVDNFDRLDERI
                     RDIQALEKEGDRIDREIGQRLEDSFVAPFDREDIHTLSGRLDDVLDYIQAVAESLSIY
                     DVQKPTDDCRELARILADQGVELGAALNKLNKVKATTVHLEKIHELEHRADGVSRAAI
                     ARLFKDRHDPLEVIKWRELYLEMENAIDAAEDAAEAIERMIHKRV"
     gene            complement(1054..2058)
                     /locus_tag="VIF08_06950"
     CDS             complement(1054..2058)
                     /locus_tag="VIF08_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY8135765.1"
                     /translation="MLDVAGLMLITVVVLAVVFDYINGFHDTANAIATSVATRALQPR
                     VAIAMATVFNFVGAFAGTAVAKTIGAGLVEEATTSTTVIAAALVGAIVWNLITWWLGL
                     PSSSSHALIGGLLGSTLVAAGTGAFKMQGIIDKVLFPMISSPLIGFVVALVLVVSLFW
                     LFRKAPRRPMTTVFRRLQILSAGFMAFSHGSNDAQKTMGIITLALFSAGAIATVDVPV
                     WVIIVAASALSLGTAVGGWRIMHTMGHRVVKLEPVHGFAAETTAATVLFTTAHFGMPV
                     STTHVISAAIMGVGTAHGVRGVRWGVARRILLAWVITLPAAGFVAAVTWLLLHFLGLG
                     "
     gene            complement(2086..2757)
                     /gene="ruvA"
                     /locus_tag="VIF08_06955"
     CDS             complement(2086..2757)
                     /gene="ruvA"
                     /locus_tag="VIF08_06955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY8135766.1"
                     /translation="MTRPTRSPWQSASPTLAPAWHSAQRVDDTLIAFVEGKVATIGTD
                     SAVLEAGGVGYRIFCGPGTLSGLKEGATVRLFTHHLVREDLQALYGFKTGEDLGFFEL
                     LITVTGVGPKVAMAIVSSRSVTDLQLAIFQGDESVLTAVSGVGKKLGARIVLELKEKV
                     SAAGAPTGAGAGSAESEVVAALQALGYSSSEARESARGAVASLPVGASLEERVKAALR
                     VLRRD"
     gene            complement(2693..3172)
                     /gene="ruvC"
                     /locus_tag="VIF08_06960"
     CDS             complement(2693..3172)
                     /gene="ruvC"
                     /locus_tag="VIF08_06960"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY8135767.1"
                     /translation="MIVLGIDPGTAATGYGVVLREGSRLTALDYGCLETLPTQQLPVR
                     LLEIHRAVTELILTHKPERLGVERLFFNKNVQTAFAVGQARGVVLLAAAEHGLPVFEY
                     GPHEVKLAVTGYGRAQKDQVQKMVQAVLRMPVLPRPDDAADALAVAICLAHARPGVA"
     gene            complement(3194..3946)
                     /locus_tag="VIF08_06965"
     CDS             complement(3194..3946)
                     /locus_tag="VIF08_06965"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8135768.1"
                     /translation="MSGHSKWSQIKRQKGANDVKRGAIFTKMGREIAIAARTGGGDPD
                     GNFRLRLAVDKARAVNMPADTIKRAIEKAAGGSAAEQFDEIVYEGYGPGGVAILVETA
                     TNNKNRTASEIRSAFTKGGGSLAGAGSVAWQFEQRGVVAVMRKGRNADDIALEAIDLG
                     ADDVDTESDPIEVITGPTSLEKLRKELEASGMKIESAELTMQAKTPIDVDLHVAEQNL
                     RLIEHLEDLEDVQRVTANFNLPAELLAEAAAV"
     gene            3973..6039
                     /gene="fusA"
                     /locus_tag="VIF08_06970"
     CDS             3973..6039
                     /gene="fusA"
                     /locus_tag="VIF08_06970"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8135769.1"
                     /translation="MPTLEMNKLRNVVIAGHSDSGKTTLAEHLLFTGGAITRLGKVTD
                     GTATLDFEPEEHKRKQSLSLAVATMNHDGYEITLIDTPGYADFAAEVVQGFEAADAAL
                     LTMDASGGVEAGTERAVALGRAHGTAALFVITRCERENADPSAALDALRASFGNKIAP
                     LHLAIGKADQFKGYVDLVHRKAYVWEKGKRSEIEIPAELSDEVARRREQLLEAAAEAD
                     DEVLTKYLEGQEISDAELDACLHRGVRDSILAPVLVTAAGHDIGIEGVLDAITRYFPS
                     PDEEKPVKAIDAKGAEVEVEQKADGPLLARVFKTTADPFVGRLTYFRVFSGKIASHDH
                     VWNADRGEEERIGQVLRVKGKDTEPVGVISAGEIGAIAKLVHTHTGDALSAREKPFKM
                     PPIEFPQPSLPVAIEPQTKADLDKMGAALSRLLEEDPGVHVERQTETGEQLLWAQGDS
                     HVAVVIERLKRKFGAAVVTHSPRIPYRETIRSKTQVEGRHKKQSGGRGQFGHIWLEIE
                     PNPGAGVEFATRIVGGSVPRQFWAGVEKGVRDVADKGPVAGFPVIDFKATLYDGSFHT
                     VDSDELSFRLAGQLATRNGIEEASPVLLEPIMDVEVQVPEAYMGDVNRDLNTRRGRVL
                     GMDSSNGTQVVRAHVPQAELFNYATELRSLSGGRGVFSQKLDHYEEVPPHVAQKVIEA
                     HKNAAA"
     gene            6215..7312
                     /locus_tag="VIF08_06975"
     CDS             6215..7312
                     /locus_tag="VIF08_06975"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY8135770.1"
                     /translation="MSRPPQSQVYTWEPSNAAIAARYGLRAEDILRFDTNTSPVAPPW
                     LPEALRGPFEPSVNEYPDSSYAEVTAAAAAYIGVEPHELVIGAGADEVLDLVAKAYLP
                     AGAAAMVPIPTYAMYGVLTGQRAARIVSVPRLGRDQGYGLDVTAMVSELPKVQVVWLC
                     SPNNPTGQLEPIDRLEQVIQTASAVPAPPLVVVDEAYFEFSGQTVVGLRSRYQNLVVV
                     RTLSKAFGLAGVRVGFGVADRSVIERLERVRPPGSVSTVSAHVAARALRAPQYALENV
                     ATLSAEREWLAAGLAARGWQPLPSTTNFLLVSIGNHDAAERAADSLLHAGLVPRTFGP
                     ANPLRGHLRLTVRSRPENERLLEVVERSQPG"
     gene            complement(7245..7766)
                     /locus_tag="VIF08_06980"
     CDS             complement(7245..7766)
                     /locus_tag="VIF08_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY8135771.1"
                     /translation="MTDATWAEFEAAAPGMAARGRELLYRGGDGEAMLVTVRGDSPPR
                     LHPVNAGVVDGHLYTFAQAKSAKRTDLDEDGRYALHTHYDPRAPHEFMLRGRARRVDD
                     QALRGAVAASWFFNAKDYPLYELLIEHALLGHRPTADDWPPVYSSWKASVTQADSSRR
                     PPAGARSPAASGP"
     gene            complement(7763..8506)
                     /locus_tag="VIF08_06985"
     CDS             complement(7763..8506)
                     /locus_tag="VIF08_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135772.1"
                     /translation="MTAQTVDDEHFFDDGGPAFLVRPTAETVLGGVLWLHWFDQDAPD
                     GNRTQFLPEAEELAGQGVVSVLPQLLFPWASDPADAEADIARIELESDRLRKAVDWLA
                     EFDAPVVFVGHDFGAMHGLLVMAHDRRIRAGVLMAPANRWANWFLRFWQVRGDRFDYL
                     RALSLFDPIERVASVAPRALLFQYAENDFFIAGMDARELHNAAGQPKRMKTYPPDHAL
                     RHLEARADRRQFILAELEASDWRKREDAR"
     gene            complement(8503..9795)
                     /gene="tyrS"
                     /locus_tag="VIF08_06990"
     CDS             complement(8503..9795)
                     /gene="tyrS"
                     /locus_tag="VIF08_06990"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY8135773.1"
                     /translation="MESGLTEASPLLRELTWRGLIAERSTGVDARLARGPVSAYVGFD
                     ASARSLHVGNLVQGFVLTHLQRAGGTPFIIVGGGTGMIGDPSGKSSERNLLDDEQIRA
                     NSAAIQRQLERFVDFSPGPNQAQMIDNREWLADWKLLDFLRDIGKHFSVPYMLAKDSV
                     QARLGAGMSFTEFSYMTLQSADFLQLYRERGVEMQMGGADQWGNITAGLELIRRVVGR
                     AEGDEPPAFAMCSPLLLTRDGKKMGKSEKGAVFLDPALTSPFDFYQYWLNDDDALVVQ
                     HLKWLTLMTEPEVGEIERQHGASPETRPAQRALAFDLTARVHGREEAERQVRVADAAY
                     GGGPITDPDVLDTLYGSLEHHDATADELAMSALDYAVASGFMASRGEARRLISQGGLA
                     INDVRITEADAPLPALIADRFLVLRSGKKRMRIDRRAQ"
     gene            9822..>11902
                     /locus_tag="VIF08_06995"
     CDS             9822..>11902
                     /locus_tag="VIF08_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8135774.1"
                     /translation="MQSRGQRRATRYDDPRAGRGNRSSSRAAVAISMLVFGGFALVGL
                     VGLIVTVGLFAIYSQGLPPTSDLEKITFGSDSVVFDRTDTIQLATFGTAENREPVTFD
                     QVPSILIDATTSTEDKSFWTNSGVDPVGIMSAMLDTIRGRERGASTITQQLVRQRLLD
                     PDLVREPGRLIERKIKEIIQSVRVTEAYPGEGGKQRIIEAYLNQNYYGNGSYGILAAA
                     RGYFGVESLSELSLGQVALLAALPKSPSTYDLVRNAEVDEFGTLYVPLNESVPIVYRR
                     NEILRTMAADPSRMVLTAGRVTPEQLLAATKEPIILAPQVAQPGQQWLAPHFIWALRD
                     ELAVKLCGPDADTCPQLEQGGLHITSSLDWNLQQVAEKWVTAAVFLPHDADPEAYAAQ
                     LGVPYESWMRKLRDLEVNNGAMIALDYQTGEILAYVGSAGYYREDATTPQFQPQFDVL
                     ANGWRQPGSAFKPFNYVTGINDGTMTAASFFMDVTTPFPDGTRTYIPKDYDLLERGPL
                     RMRSALQFSLNIPAVKAQVINGTDHIFDMAQRFGMTFQGVTPRAGLSLTLGTEVTHIA
                     DVAESFGTLANEGRYIGYTHVLRITDSAGTDLIPPYAPPAGETVVSPQAAYVMTDILG
                     SNTRPGQNPIWGAFELTGPNGQHRPATLKTGTNNDANDLVAFGYTAPPGDEARAAGQY
                     ALVVGAWSGNS"
BASE COUNT         2015 a         4035 c         3961 g         1891 t
ORIGIN      
        1 gcgctggcgg gcagctgcga gcagtatctc gaggttctgc ttgacggtcc cctgaccgat
       61 gtactcggcc agcaggcgtg gtcggagaga cgtctcgaca gcgacttctt cgaccgacag
      121 ggtgcccgtg acgacggggc ggtcgggctc caggccggat tcctgcatcg ggcgtcagtc
      181 taccaggttt tgacgggtat agaacgcatg ttcgacgctc cgtggtccag ttcccagcac
      241 atgtgctgac tggtcaacct tatgcgtcac ggcgggtcca cagagccggg caaagcgacg
      301 gatccggcgg ttgcccacgg tcagcggcac atcccaaagt cccaatccgc cggagcgggc
      361 acgcctaagg ctgacaggcg agcacatttg ccgggatcgg cgcgcgggcg gggttcgaac
      421 gggtcacacc ctcttgtgga tcatgcgctc gatcgcctcc gcggcgtcct ccgccgcgtc
      481 aatcgcgttc tccatctcga gatagagctc gcgccacttg atgacctcaa gcggatcatg
      541 gcggtccttg aaaaggcgcg cgatcgcggc tcgcgagacg ccgtctgccc ggtgctccag
      601 ctcatgaatc ttctcgaggt gcacggtcgt ggccttgacc ttgttcagct tgttcagcgc
      661 cgcgcccagc tcgacgccct gatcagccag gatgcgcgcc agctctcgac aatcgtcggt
      721 gggcttctga acgtcgtaga tcgacaggct ctcggccacg gcctggatgt agtcgagcac
      781 gtcgtcgagg cggccgctca gggtgtggat gtcttcgcgg tcgaagggcg caacgaagga
      841 gtcctcaagt cgctggccga tctcgcgatc gatgcggtcg ccttccttct cgagcgcctg
      901 gatgtcgcgg atgcgctcgt cgagccgatc gaagttgtcg acgagctcgt gcagtgcctt
      961 gccggcggca gcgacgttct caccgcccgc ctcgaatagg tcgaagaact ttatgtcgcg
     1021 cgggatcagt cgcaggctca ccgggttacc tcctcagcca agtccaagaa aatgaagcag
     1081 cagccacgtg accgccgcca cgaagcctgc cgcgggaagg gtgatgaccc aagccagcag
     1141 gatgcgccgg gccacgcccc agcgcacgcc gcgtaccccg tgcgccgtgc cgacacccat
     1201 gatcgccgcc gagatgacgt gcgtcgtcga gaccggcatt ccgaaatggg ccgtcgtgaa
     1261 cagcacggtg gccgccgtcg tctcggccgc gaagccgtgg accggctcaa gcttgacgac
     1321 gcggtgaccc atggtgtgca tgatccgcca gccgccgacc gccgttccca gggacagcgc
     1381 cgaagcagcc acgatgatca cccagaccgg cacatccacg gtggcgatcg ccccggccga
     1441 gaacagcgcc agggtgatga tgcccatcgt cttctgggca tcgttggagc catgcgagaa
     1501 ggccatgaag ccggccgaca ggatctgcaa cctccggaag acggtggtca tcggccggcg
     1561 cggcgccttg cggaagagcc agaacaggct gaccaccagc acgagggcga ccacgaaacc
     1621 gatcagtggc gagctgatca tcgggaagag caccttgtcg atgatgccct gcatcttgaa
     1681 cgcgccggtg ccagcggcga ccagcgtaga acccagcaac cccccgatca gggcgtggct
     1741 gctcgagctg ggcaacccga gccaccacgt gatcaggttc cacacgatcg ccccgacaag
     1801 ggcggcggcg atgaccgtgg tgctggtcgt cgcctcctcc acgagtccgg cgccgatggt
     1861 ctttgccacc gccgtcccgg cgaacgcgcc gacaaagttg aagactgtgg ccatggcgat
     1921 cgcgacgcgg ggctgaaggg cgcgtgtcgc aaccgaggtc gcgatcgcgt tcgccgtgtc
     1981 gtggaagccg ttgatgtagt cgaacaccac ggccaggacg acgaccgtga tcagcatgag
     2041 acccgcgacg tcgagcattc agacggagac tacctgcgct tgcggctagt cgcgccggag
     2101 gacccgcagc gcggccttga cgcgctcttc gagtgacgcg cccacgggca gcgacgcgac
     2161 cgcgccgcgc gccgactccc gcgcctctga tgacgaataa ccgagcgcct ggagagccgc
     2221 cacaacctct gactctgccg atccagcccc ggctccggtc ggtgcgccgg ccgccgacac
     2281 cttctccttg agctcgagca cgatgcgcgc cccgagcttc ttgccgacgc ccgacaccgc
     2341 cgtgagcacg ctctcgtcgc cctggaagat cgccagctga aggtcggtga ccgaacgcga
     2401 tgagacgatg gccatcgcca ccttgggccc gacgccggtg accgtgatca gcagctcgaa
     2461 gaagcccagg tcctcgccgg tcttgaagcc gtacagcgcc tgcaggtcct cgcgcaccag
     2521 gtgatgcgtg aagaggcgca ccgtcgcgcc ctccttcagg ccgctcagcg tccccggccc
     2581 gcagaagatc cggtagccca ctccgccggc ctccaacacg gccgaatcgg taccgatggt
     2641 cgcgaccttg ccctcgacga acgcgatcaa cgtgtcatca acgcgctgcg cgctatgcca
     2701 cgccggggcg agcgtgggcg aggcagattg ccacggcgag cgcgtcggcc gcgtcatcgg
     2761 gacgcggcag gaccggcatc ctgagcacgg cctggaccat cttctggacc tggtccttct
     2821 gggcgcggcc gtagccggtc acggcgagct tgacctcatg cggcccgtac tcgaagaccg
     2881 gcagcccgtg ctcggcggcc gcgagcagga cgacgccgcg tgcctggcca acggcgaacg
     2941 cggtctggac gttcttgttg aagaagagcc gctcgacgcc aaggcgctct ggcttgtggg
     3001 tcaggatcag ctcagtcacc gcgcgatgga tctcgagcag gcggaccggc agctgctgcg
     3061 tcggcagtgt ctccaggcag ccgtagtcga gggccgtcag tcgagagccc tcgcgcagta
     3121 cgacgccgta gccggtcgcg gcagtgccgg ggtcgatgcc gaggacgatc acctacctag
     3181 cctagggaaa gggctagacg gccgcagcct cggccagcaa ctccgcgggc aggttgaagt
     3241 tggccgtgac gcgctggacg tcctcgaggt cctcgagatg ctcgatcagg cgcaggttct
     3301 gctcagccac gtggaggtcc acgtcgatgg gcgtcttggc ctgcatcgtc agctcagccg
     3361 actcgatctt catgcctgat gcctcgagct ccttgcgcag cttctccagg ctggtcggtc
     3421 cggtgatgac ctcgatcggg tccgattcgg tatcgacatc gtccgcgccc aggtcgatcg
     3481 cttcgagcgc gatgtcgtcg gcgttcctgc ccttgcgcat gacggcgaca acgccgcgct
     3541 gctcgaactg ccaggcgacg ctgccggcgc cggcgagcga gccgccgccc ttggtgaatg
     3601 ccgagcgaat ttcgctcgcg gtgcggttct tattgttcgt cgcggtctcg accaggatcg
     3661 cgacaccgcc ggggccatag ccctcgtaaa cgatctcgtc aaactgctcc gcggctgagc
     3721 cgcccgcagc tttctcgatc gcgcgcttga tggtgtctgc gggcatgttc acggcgcgcg
     3781 ccttgtcgac tgcgaggcgc agccggaagt tgccgtcagg gtcgccgccg cccgtgcgcg
     3841 ccgcaatcgc gatctcgcgg cccatcttgg tgaagatggc gccgcgtttg acgtcgttgg
     3901 cgcctttctg gcgcttgatc tgggaccact tggaatgacc ggacatcgcg accgattcta
     3961 tatgctgcgc ccatgcccac ccttgagatg aacaagctcc gtaacgtcgt tatcgccggt
     4021 cactccgatt cgggaaagac caccttggcc gagcacctgc tcttcaccgg cggggcgata
     4081 acccggctgg gcaaggtcac cgatggaacg gccacgctcg atttcgagcc ggaggagcac
     4141 aagcgcaagc agtcgctctc cttggcagtg gcgacgatga accatgacgg ttacgagatc
     4201 acgctcatcg atacgcccgg ctacgccgat ttcgcggcgg aagtggtcca gggcttcgag
     4261 gccgcagatg ccgcgctact gacaatggac gcctccggtg gtgtcgaggc gggcactgag
     4321 cgcgcggtcg cgctgggccg cgcacatggg acggcggcgc tcttcgtgat cacccgctgc
     4381 gaacgcgaga acgccgaccc atccgcggcc ctcgatgccc tgcgcgcgtc cttcggcaac
     4441 aagatcgcgc cgctccacct ggccattggc aaagcggacc agttcaaggg ttacgtggac
     4501 ctggtgcatc gcaaggcgta tgtctgggag aagggcaagc gctccgagat cgagatcccg
     4561 gccgagcttt cggacgaggt ggcgcgccgg cgcgagcagc tgctcgaggc cgcggcagaa
     4621 gcagatgacg aggtgctgac caagtacctc gagggccagg agatcagcga cgccgagctc
     4681 gatgcgtgcc tccaccgcgg cgtgcgcgac tcgatcctgg cgcccgtcct cgtcaccgcg
     4741 gcgggccacg acatcggcat cgagggcgtg ctcgacgcga tcacgcgcta ttttccctcc
     4801 cccgatgagg agaagccggt caaggcgatc gacgccaagg gtgccgaggt cgaagttgaa
     4861 cagaaggcgg acggcccgct cctggcgcgc gtcttcaaga ccaccgctga cccgttcgtg
     4921 ggccggctca cctacttccg cgtgttcagc ggcaagatcg cctcgcacga ccacgtgtgg
     4981 aacgcggacc gcggcgagga agagcgcatc ggccaggtcc tgcgcgtcaa gggcaaggac
     5041 acggagcctg tgggtgtcat ctcagccggc gagatcggcg cgatcgcgaa gctcgtccac
     5101 acgcacaccg gtgacgccct gtcggcgcgc gagaagccat tcaagatgcc gccgatcgag
     5161 ttcccgcagc catcgttgcc agtggcgatc gagccgcaga ccaaggccga cctggacaag
     5221 atgggcgcgg cgctgtcccg gctgctcgaa gaggatccgg gcgtgcacgt cgagcgccag
     5281 acagagaccg gcgaacagct gctgtgggcg cagggcgact cacacgtcgc ggtggtcatc
     5341 gagcggctca agcgcaagtt cggcgccgcc gtcgtcactc actcgccgcg catcccctat
     5401 cgcgagacga tccgctccaa gacccaggtc gaggggcgcc acaagaagca gtcaggcgga
     5461 cgcggacagt tcggccacat atggctggag atcgaaccca accccggcgc cggtgtcgag
     5521 tttgcgacgc gcatcgtcgg cgggtcggtg ccgcgccagt tttgggcggg cgtcgagaag
     5581 ggcgtgcgcg acgtcgccga caagggaccc gtcgccggct tcccggtgat cgacttcaag
     5641 gcaacgctct acgacggctc gttccacacg gtcgactcgg acgagctgtc tttccgattg
     5701 gccgggcagc tggcgacgcg caatgggatc gaggaggcat caccggtcct gctcgagccg
     5761 atcatggacg tcgaggtgca ggtcccggag gcgtacatgg gcgacgtcaa ccgcgacctc
     5821 aatacgcgcc gcggccgcgt gctgggcatg gactcgtcca acggcacgca ggtcgtgcgt
     5881 gcccacgtgc cccaggccga gctcttcaat tacgcgaccg agctgcgctc gctgtccggt
     5941 ggccgcggcg tcttcagcca gaagctggat cactacgagg aagtgccgcc acacgtcgcc
     6001 cagaaggtca tcgaggcgca caagaacgca gcggcctagc gccgcgaacg tcgcggcgcg
     6061 ctgctgggtc ctttccgcca aatgcagggc tggtctgcac gggaatcggt cttgtctgtc
     6121 agcggcctga ccagtcctgc gcctgacgga ggattcggcc gatttcgaga ttcggtgcct
     6181 acccagtgct gcatttgaca ggtcggcgcg accgatgagc cgcccgccgc agtcgcaggt
     6241 ctacacgtgg gagccgtcga acgcggccat agcggcacgc tacgggctcc gggccgagga
     6301 catcctgcgc ttcgacacca atacgtcgcc ggtcgcgccg ccgtggctcc cggaagcgct
     6361 gcgcggccct ttcgaaccga gcgtcaatga gtaccccgac tcgtcctacg cggaggtgac
     6421 cgcggcggcg gccgcgtaca tcggcgtcga gccgcacgag ctggtgatcg gcgcgggcgc
     6481 ggacgaggtg ctcgacctcg tcgccaaggc gtacctgccg gccggcgccg cggcgatggt
     6541 gccgatcccc acctacgcga tgtacggcgt cctgaccggc cagcgcgcgg cacgcatcgt
     6601 gtccgtaccg cggctgggac gcgatcaggg ctacgggctc gacgtcacag cgatggtcag
     6661 cgaactgccc aaggtgcagg tcgtctggct gtgctcgccc aacaacccga ctggtcagct
     6721 cgagccgatt gaccggctgg aacaggtcat ccagacggca tccgccgtgc ccgcgccgcc
     6781 tctcgtcgtg gtcgacgagg cgtatttcga gttcagcggc cagacggtgg tcgggctgag
     6841 gtcgcgctac cagaacctgg tcgtcgtcag gacgctgtcg aaggcgtttg gactggctgg
     6901 cgtacgggtg ggtttcggtg tggccgatcg gtcggtcatc gaacggctcg agcgcgtccg
     6961 gccaccgggg agcgtctcaa cggtgtccgc ccacgtcgcg gcgcgggcgc tgcgcgcgcc
     7021 gcagtacgcg ctggagaacg tggcgacgct gtccgcggag cgcgagtggc tcgccgccgg
     7081 cctggcggca cgcggctggc aaccattgcc cagcaccacg aacttcctgc tcgtctcgat
     7141 aggcaaccac gatgccgccg agcgagccgc cgacagcctg ctgcacgcag gcctcgttcc
     7201 gcgcaccttc ggaccggcca acccgctgcg cggccacctg cggcttacgg tccgctcgcg
     7261 gccggagaac gagcgcctgc tggaggtcgt cgagaggagt cagcctgggt gactgacgcc
     7321 ttccaggagg aatagaccgg cggccagtcg tcggcggtcg ggcgatggcc gagtagcgcg
     7381 tgctcgatca gcagctcgta cagcgggtag tccttggcgt tgaagaacca gctcgccgcc
     7441 accgcgccgc gcaacgcctg atcgtccacg cgtcgggcac ggccgcgcag catgaactcg
     7501 tgcggcgccc gcgggtcata gtgggtgtgc agggcgtagc ggccgtcctc gtccaaatcg
     7561 gtgcgcttgg ccgacttcgc ctgcgcgaag gtataaaggt ggccatcgac aacaccggcg
     7621 ttgaccggat gaagacgcgg cggcgaatcg ccgcggaccg tgacgagcat cgcctcgccg
     7681 tcgccgccgc gatagagcag ctcgcgaccg cgcgccgcca tccctggcgc cgcggcttcg
     7741 aattcagccc acgtcgcgtc tgtcatcgag cgtcctcgcg ctttcgccag tcgctcgcct
     7801 cgagctccgc gaggatgaac tggcgccggt cagcgcgcgc ctccaggtgg cgcagcgcat
     7861 ggtctggtgg ataggtcttc atgcgcttgg gctggcccgc cgcgttgtgg agctcgcgcg
     7921 catccatgcc ggcgatgaag aagtcgttct ccgcgtattg gaagagcagc gcccgaggcg
     7981 ccacggatgc gacgcgctca atgggatcaa acaggctcag ggcgcgcagg tagtcgaatc
     8041 gatcgcccct gacctgccag aaccgcagga accagttcgc ccaccggttg gccggggcca
     8101 tcagcacgcc ggcccggatc cggcggtcgt gcgccatcac cagcagcccg tgcatcgccc
     8161 cgaagtcgtg accaacgaag acgacgggcg cgtcgaactc ggccagccag tcgaccgcct
     8221 tgcgcagccg gtcgctctcg agctcgatcc gggctatgtc tgcctctgca tcggccggat
     8281 ccgacgccca cggaaacagc agctgcggca gaaccgacac gacaccctgg cccgccagct
     8341 cctccgcctc cggcaggaac tgcgtgcgat tgccgtcggg cgcgtcttga tcgaaccagt
     8401 gcagccacag cacaccaccg aggacggtct cggcggttgg acggaccagg aaggctggtc
     8461 caccgtcgtc gaagaagtgc tcgtcgtcga cggtctgagc cgtcattgcg cgcggcggtc
     8521 gatgcgcatg cgcttcttgc ccgagcgcag caccaggaag cgatcggcga tgagagcggg
     8581 cagcggcgcg tcggcctcag tgatgcggac gtcgttgatg gcgaggccgc cctggctgat
     8641 caggcggcgc gcctcacccc tggaggccat gaagccgctt gcgaccgcgt aatcgagagc
     8701 gctcatcgcc agctcgtccg cggtcgcatc gtggtgctca agggagccgt agagcgtgtc
     8761 gaggacgtcc ggatcggtaa ttggccctcc gccataggct gcgtccgcga ctcgaacctg
     8821 tcgctccgcc tcttccctgc cgtgcacccg cgcggtcagg tcgaaggcca gcgcgcgctg
     8881 cgccgggcgc gtctccggcg acgcgccgtg ctgccgctcg atctccccga cctctggctc
     8941 tgtcatcagc gtcagccact tgaggtgctg cacgaccagg gcgtcgtcgt cgttgagcca
     9001 gtactggtag aagtcgaagg gcgaggtcag tgccggatcg aggaacaccg cgcccttctc
     9061 gctcttgccc atcttcttgc cgtcgcgcgt cagcagcaac ggtgagcaca tcgcgaaggc
     9121 cggtggttcg tcaccttccg cgcggccaac gacgcgccgg atcagctcta gcccggcggt
     9181 gatgttgccc cactggtcgg cgccgcccat ctgcatctcg acgccgcgct cgcggtaaag
     9241 ctgcaggaag tcggccgact gcagggtcat gtacgagaac tcggtgaacg acatcccggc
     9301 gcccaggcgt gcttggaccg agtccttggc gagcatgtag ggcacggaga agtgcttgcc
     9361 gatgtcgcgc aagaagtcga gcagcttcca gtcagcgagc cattcgcggt tgtcgatcat
     9421 ctgggcctgg ttggggccag gcgagaagtc gacgaagcgc tccaactgcc tctggatcgc
     9481 cgccgagttg gcgcggatct gctcgtcgtc gagcaggttg cgctcgctcg acttgcccga
     9541 cgggtcgccg atcatcccgg tcccgccgcc gacgatgatg aacggcgtgc cgccggcgcg
     9601 ctggagatgg gtgagcacga agccctggac gagattgccc acgtggagtg agcgcgcgga
     9661 cgcgtcaaaa ccgacataag cgctaacggg accgcgcgcg aggcgcgcgt cgacgcccgt
     9721 cgagcgctcc gcgatgaggc cgcgccacgt gagctcacgc aggagcggag atgcttccgt
     9781 aaggcccgat tccatcgcct aatgctagag tctgggcgca gatgcagagc cgcgggcaac
     9841 gccgggcgac gcgctacgac gatccacggg ccgggcgcgg caaccgatcg tcgagtcgcg
     9901 cggcagtcgc catttccatg cttgtctttg gcggattcgc gctggtcggt ctggttgggc
     9961 tgatcgtgac ggtcggcctt ttcgccatat acagccaggg cctgccgccc acgagcgacc
    10021 tcgagaagat cacctttggc tccgattcgg tcgtcttcga ccggaccgac accatccagc
    10081 tggcgacgtt cggaaccgcg gagaatcgcg agccggtcac attcgaccag gtgccgtcca
    10141 tcctgatcga tgccacgaca tcgaccgagg acaagtcgtt ctggacaaac agcggcgtcg
    10201 atccggtcgg gatcatgagc gcgatgctcg acaccatccg cggccgcgag cgcggcgcat
    10261 cgaccatcac ccagcagctc gtccgccagc gcctcctcga cccagacctg gtgcgcgaac
    10321 ctggccggct gatcgagcgc aagatcaagg agatcatcca gtcggtgcgc gtgacggagg
    10381 cctatccggg cgaaggcggc aagcagcgca tcatcgaggc ctatctcaac cagaactact
    10441 acggcaacgg ctcgtacggc atcctcgcgg cggcgcgcgg ctacttcggc gtcgagtcgc
    10501 tgagcgaact gtcgctgggc caggtggcgc tgctcgcggc gctgcccaag tcgccctcga
    10561 cctacgacct ggtgcgcaac gcggaagtcg acgagttcgg cacgctgtat gtgccgctga
    10621 acgagagcgt gccgatcgtc taccgacgca acgagatcct gcgcacgatg gcagcggatc
    10681 ccagtcgcat ggtgcttaca gcgggtcggg tcacgcctga acagcttctg gccgcgacga
    10741 aggagccgat catcctcgcg ccccaggttg cgcagcccgg ccagcaatgg ctcgcgccgc
    10801 acttcatctg ggcgctgcgc gatgagctgg cggtgaaact gtgcgggccc gacgccgaca
    10861 cctgcccgca gctcgagcag ggcggcctgc acatcacctc atcgctcgac tggaatctcc
    10921 agcaggtggc agagaagtgg gtgacggcag ccgtcttcct gccgcacgac gctgatccgg
    10981 aggcatatgc cgcgcagctc ggcgtgccct acgaatcgtg gatgcgcaag ctgcgcgatc
    11041 tcgaggtcaa caacggcgcg atgatcgcgc tcgactatca gactggcgag atcctggcgt
    11101 acgtcggtag cgccggctac taccgcgagg acgcgacgac gccccagttc cagccccagt
    11161 tcgacgtgct cgccaacggc tggcgtcagc cgggcagcgc cttcaagccc ttcaactacg
    11221 tgaccgggat caacgacggc acgatgaccg ccgcatcgtt cttcatggac gtgacgacgc
    11281 ccttccccga cggcacgcga acctacatcc ccaaggacta cgacctgctc gaacgcggac
    11341 cattgcgcat gcgcagcgcg ctgcagttct cgcttaacat cccggcggtc aaggcgcagg
    11401 tgatcaacgg caccgaccac atttttgata tggcccagcg tttcggtatg accttccagg
    11461 gtgtgacgcc gcgcgccggc ctgtcgctga ccctgggcac ggaagtgacg cacatcgccg
    11521 acgtcgccga gtcgttcgga acgctcgcca acgagggccg ctacatcggc tacacgcacg
    11581 tcctgcgcat caccgattcg gccggcacgg atctcattcc gccgtacgcg ccgccggccg
    11641 gcgagaccgt cgtctcaccc caggcggcct acgtcatgac cgacatcctg gggtcgaata
    11701 cgcgacctgg ccagaatcca atctggggcg cgttcgagct gaccgggccc aacggccagc
    11761 accgcccggc aacgctcaag acgggcacca acaacgacgc gaacgacctc gtcgcgttcg
    11821 gctacacggc gccacccggt gacgaggcgc gggcggcggg ccagtacgcg ctcgtggtcg
    11881 gggcgtggag cggcaacagc ga
//
LOCUS       DATBRX010000180         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3085747, whole genome shotgun sequence.
ACCESSION   DATBRX010000180 DATBRX010000000
VERSION     DATBRX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3085747"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /gene="uvrC"
                     /locus_tag="VIF08_07000"
     CDS             <1..1645
                     /gene="uvrC"
                     /locus_tag="VIF08_07000"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY8135775.1"
                     /translation="SVSEALLLEATLIKRHQPRFNVRLKDDKSYPYIKVTLGDDFPRI
                     ERTRKLPNDGSRYFGPYASASSVDEAMNLIRRLFPFRTCTIPISEGKRALPRPCLLYH
                     IKRCQGPCIEAISKADYRADIDQVMLFLEGRSEQVAKRLREEMTRASESTDYERAADM
                     RDKLRAIERTMESQKMAAFAKTELDVLGYARQGGGAAVQLFAIRDGTTVARDIFLLEN
                     VGDSADVEALSAFVKQYYAAASSIPPRVLVPWPLPDSAELTSFLEARRGHRVAIDVPQ
                     RGERRALMQLAERNAAETLAREQARWLADQGKTLAALEELAGALGLPAPPLRIECYDI
                     STIQGTNTVGSMVVFEEGRPRSGEYRRFRVRTTAVAPGRPDDYASHREVLRRRFYRAL
                     EAEEGAAEELRWRLPDLVVIDGGLGQVNAARGVLDELGLHDLPAIGLAKEREEIFLPG
                     LSTPLVLPANSQALYLVQRLRDEAHRFAITYHRKLRAKSQVRSVLDDVAGVGPARKRA
                     LLRVFGSTRQMRSASVDEIASVPGISRALAETIHSALNG"
     gene            1674..3041
                     /gene="secD"
                     /locus_tag="VIF08_07005"
     CDS             1674..3041
                     /gene="secD"
                     /locus_tag="VIF08_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY8135776.1"
                     /translation="MRRLLPLVILVVGFAAVAVDFARLPRPFTEEPCTATVADGCIDT
                     KLGLDLQGGLRGEYRALPANGVPVSSTALANIRTIIENRINKFGVSEPVVQTQGSDRI
                     VVEIPGVSDEQQVRAVIGSTGQLLFVDVPANRSNQVIPGQPIPGDLTIIFGGDRIKSA
                     APGFTDTGLRAVDVTMTEEGARLFDQYAATHFGQQFAIVLDGIVQSAPVIRAQRFGGK
                     AQISGSFSSQAEVNQLVTVLNYGALPNAVEEVSFSKISATLGFNFLQQSILAGAIGIA
                     LVFVFMLLHYRLPGLVACLALIYYALVVYAIFRLVPVTLTLAGVAAFVLSVGMAVDAN
                     ILIFERTKEELRSGKTLVSAVEAGFNRAWNSIFDSNMSSIITAAILFYFGSATIRGFA
                     LVLIIGVLVSMFTAVTLSRQMLRFVVHQPWARKARYFGVADDEFLVTAPSARTRTRRE
                     AAADV"
     gene            3034..>3569
                     /locus_tag="VIF08_07010"
     CDS             3034..>3569
                     /locus_tag="VIF08_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135777.1"
                     /translation="MFDIIGRRRWFYIFSLLITVPGLIFILLTPISGGKVGLQFSIDF
                     TGGTVWEVHFKNGTPTGQAVQEVMAAQGLPGSVAITTSGDKEYVLIRTVQIGLRPQPS
                     PSVAPSGSPATSGSPVASGSPGPSGSPRPSGSPGASATPTPAPSATTSATPSASPSAA
                     ASPSPSAAASPSASPAVSP"
BASE COUNT          557 a         1256 c         1184 g          572 t
ORIGIN      
        1 cagcgtcagc gaggcgctgc tgctcgaagc cacgctgatc aagcgccatc agccgcgctt
       61 caacgtgcgg ctgaaggatg acaagtcgta cccgtacatc aaggtgacgc tcggcgacga
      121 cttcccgcgc atcgagcgga cacgcaagct gcccaacgac ggctcgcgct acttcgggcc
      181 atacgcgagc gccagcagcg tcgacgaggc gatgaacctc atccgccgcc tgttcccgtt
      241 ccggacgtgc acgatcccga tcagcgaggg caagcgggca ctgccccgac cctgcctgct
      301 gtaccacatc aagcgctgcc agggcccgtg catcgaggcc atttcgaagg cggactatcg
      361 ggccgatatc gaccaggtga tgctcttcct cgagggacga tcagagcagg tcgccaagcg
      421 gctgcgcgag gagatgaccc gggcatcgga atcgaccgat tacgagcggg cggccgacat
      481 gcgcgacaag ctgcgcgcca tcgagcggac gatggaatcg cagaagatgg cggcgttcgc
      541 gaagacggag ctggatgtcc tgggctacgc gcgccagggc ggcggcgcgg ccgtgcagct
      601 gttcgcgatc cgcgacggca cgacggtggc gcgcgacatc ttcctattgg agaacgtcgg
      661 tgactccgcg gatgtggagg cgctgtcggc gttcgtgaag cagtactacg ccgccgcgtc
      721 gtcgattccg ccgcgcgtcc tggttccctg gccgctgccc gactcagcgg agctcacgtc
      781 cttcctggag gcgcggcgcg gtcaccgtgt cgcgatcgac gtgccgcagc gaggcgagcg
      841 acgggcgctg atgcagctcg ccgaacggaa tgcggcggag acgctggcgc gcgagcaggc
      901 gcgctggctg gccgaccagg gcaagaccct cgccgcgctc gaagagctcg cgggcgccct
      961 tggcctgccg gcgccgccac tgcgcatcga gtgctacgac atctcgacga tccagggcac
     1021 gaacaccgtc ggcagcatgg ttgtgttcga ggagggccgg ccacgttcgg gcgagtaccg
     1081 ccggttccgc gtccgcacga ctgcggtcgc acccggccgg cctgacgact acgccagcca
     1141 ccgcgaggtg ctgcgccggc gcttctaccg cgcgctcgag gccgaggagg gcgccgcgga
     1201 ggagctgcgc tggcggctgc cggacctggt cgtgatcgac ggcggcctgg gccaggtcaa
     1261 cgcggcgcgc ggggtccttg acgagctggg cctgcacgac ctgccggcga tcgggctggc
     1321 caaggagcgc gaggagatct tcctgccggg cctgtccaca ccgctcgtcc tccctgcgaa
     1381 ctcgcaggcc ctgtacctgg tccaacggct gcgcgacgag gcgcatcggt tcgcgatcac
     1441 gtaccaccgc aagctgcgcg caaagtcgca ggtccggtca gtgctggacg acgtggccgg
     1501 tgtcggtccg gccaggaagc gcgccctgtt gcgcgtcttc ggctcgacca ggcagatgcg
     1561 ctcggcgtcg gtcgacgaga tcgcgtcggt cccgggcatc agccgcgcgt tggcagagac
     1621 gatccactcc gcgctgaacg gctgagcggt tactcggcta acatcccggc cgaatgcgca
     1681 ggctccttcc ccttgtgatc ctcgtggtcg gattcgccgc ggtcgccgtc gatttcgcgc
     1741 gcctcccccg gccgttcacg gaggagccct gcacggcgac ggtggccgac ggctgcatcg
     1801 acacgaagct cggccttgac ctgcagggcg gcctgcgcgg cgaataccgc gccctgcccg
     1861 ctaatggcgt gccggtgtca agcacggccc tggccaacat ccgcacgatc atcgagaacc
     1921 gcatcaacaa gttcggcgtg tccgagcccg tggtgcagac gcagggcagt gaccgcatcg
     1981 tggtcgagat cccgggcgtg agcgatgagc aacaggtgcg cgccgtgatc ggctcaaccg
     2041 gccagctgct ctttgtcgat gtcccggcca accgatccaa ccaggtcatt cccgggcagc
     2101 ccattccggg cgatctcacg atcatcttcg gcggcgaccg gatcaagagc gcggcgccgg
     2161 gcttcaccga cacgggcctg cgcgccgtcg acgtcacgat gaccgaggag ggcgcgcggc
     2221 tgttcgacca atacgccgcg acgcacttcg gccagcagtt cgcgatcgtg ctcgatggga
     2281 tcgtccagtc cgcccccgtg atccgggcgc agcgctttgg tggcaaggcg cagatctcgg
     2341 gcagcttctc gtcccaggcc gaggtgaatc aactcgtcac ggtgctcaac tacggcgcgc
     2401 tgccgaacgc ggtcgaagag gtcagcttca gcaagatcag cgcgaccctc ggcttcaact
     2461 tcctgcagca aagcatcctc gccggcgcca ttggcatcgc gctggtcttc gtgttcatgc
     2521 tcctgcacta ccgattgccc ggcctggtgg cctgcctcgc cctgatctat tacgcgctcg
     2581 tcgtctacgc catcttccgg ctggtgccgg tcaccctcac cctcgccggg gtggcggcgt
     2641 tcgtgctgtc ggtgggcatg gcggttgacg ccaacatcct gatcttcgag cgcaccaagg
     2701 aagagctgcg ctccggcaag acgctcgtgt cggcagtcga ggcgggcttc aaccgggcct
     2761 ggaactcgat cttcgactcg aacatgtcga gcatcatcac ggccgccatc ctcttctact
     2821 tcggctcggc gacgatccgc ggctttgccc tggtgctgat catcggcgtg ctcgtgtcga
     2881 tgttcaccgc cgtcacgttg agccggcaga tgctgcgctt cgtcgtccac cagccgtggg
     2941 cccgcaaggc gcgctacttc ggagtcgcgg acgacgagtt cctggtcacc gcacccagcg
     3001 cgcggacgcg cactcggcgc gaggcagcag ccgatgtttg acatcatcgg caggcgccgt
     3061 tggttctaca tcttcagcct gctgatcacg gtccccggcc tgatcttcat cctgctcacg
     3121 cccatcagcg gcggcaaagt cgggctgcag ttctcgatcg acttcaccgg cggcacggtc
     3181 tgggaggtgc acttcaagaa cggcacgccc acgggccagg cggtccagga ggtcatggcc
     3241 gcgcagggcc tgccgggcag cgttgccatc accacgtcgg gcgacaagga gtacgtcctc
     3301 atccggacag tccagatcgg gttgcgcccg cagccgtcgc catcagtggc gccctccggc
     3361 tcacccgcga cgtccggttc acctgtcgcg tcgggctcac ccgggccctc aggctcaccc
     3421 aggccctcag gctcacccgg cgcgtcggcc accccgacgc ccgcgccaag tgccacgacg
     3481 agtgcgacgc ccagcgcgag cccatctgcg gcagccagtc ccagcccatc cgcggcagcg
     3541 agtcccagcg cgtcgcccgc ggtcagccc
//
LOCUS       DATBRX010000181        21340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1187379, whole genome shotgun sequence.
ACCESSION   DATBRX010000181 DATBRX010000000
VERSION     DATBRX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21340
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187379"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VIF08_07015"
     CDS             complement(<1..516)
                     /locus_tag="VIF08_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8135778.1"
                     /translation="MSARFTWFGHSCVELRTPGDKVVLFDPWFGNPTSTRAAADLERC
                     DVMLVSHGHFDHMGSEPRHVSDANALTIARRTNPAWPCIHELSLWLETELEGAGVEVI
                     GMNKGGTVQTRGLSVSMVHADHSAGDWPALGPMASRSGLGSGPLYLGEPVGFVVGLED
                     GRRVYFAGDTDV"
     gene            634..1650
                     /locus_tag="VIF08_07020"
     CDS             634..1650
                     /locus_tag="VIF08_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEY8135779.1"
                     /translation="MDFLPAIFLVLLIICAIGALLVTAVPDQRAHQLQLQSRRFGNDA
                     GDKGEVQVRYPFRRTLIAGAFAFLVLWIVSLGVVIVPAGFVGVVRFLGQVQPNVLYPG
                     ASWVVPFVNTVEEVDTRVQPHNFQQLTAATAENLQVTVTGTMNYHLAAAFAGQLIQNV
                     GTDFASKIIDPAFSDYIKQVTPAYSADSNSPNFILNKRDEIRQLTKQQLGDNLARYGI
                     IVDDIYIVDIGYPQAYNDAITQKQVEQQNVQREQQILEQRRIQAQQVVVTAQGAADAL
                     RETAAGQADANASINSSLTDELIRWQAIQKLNPNVQIMLIPSDNNFILPLPSLEPVTP
                     TPVP"
     gene            1677..2285
                     /locus_tag="VIF08_07025"
     CDS             1677..2285
                     /locus_tag="VIF08_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8135780.1"
                     /translation="MATNLYVLGDEPSGEAIAIDTATPCVEWLTATLAERGWRLKLVV
                     STHRHWDHIGDNAAVSAATGAGIAAHIADRHGLEHPTGHLAPFPIPPSVPALDLAEGS
                     TIRFGEITLTILHTPGHTEGSVCLMADSEGLLFTGDTLFAGSWGRTDLPGGSEEAMVD
                     SLGRLAGLGDDLKVLPGHGSQTTLAAEKPWLEQVIRTRLLPF"
     gene            complement(2282..3364)
                     /gene="rsgA"
                     /locus_tag="VIF08_07030"
     CDS             complement(2282..3364)
                     /gene="rsgA"
                     /locus_tag="VIF08_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY8135781.1"
                     /translation="MTVLERWGWTEREATLFQPHLDAALQPGRVLSEHRGGYVVAAEG
                     GDVDGSVSGRFRFDAAGSEDFPAVGDWVALEVAPGSDTAVIHAVLPRMTRFARPARGD
                     IAAAQVVAANVDLVLLVTALDHDFNLRRLERYLALAWSSGAEPVIVLNKADLCDDVAG
                     RIADVSLVAPGVSVRALSARDRTGIESLEPLLEAGKTVALLGSSGVGKSTIVNALLGY
                     ERQQTGEVRADDQRGRHTTTMRELLAMPSGALLIDSPGMRSVGMWEVEEGLADAFADI
                     EEHAAGCRFGDCTHTGEPGCAVQQAIADGSLPAARLESQRKLARESAALAARVDPVAR
                     AAHRKRWTAIHKSVRTHMRTKYGADA"
     gene            complement(3364..4380)
                     /locus_tag="VIF08_07035"
     CDS             complement(3364..4380)
                     /locus_tag="VIF08_07035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135782.1"
                     /translation="MNEKTATTERRAGLPGLTLRPYAGEADLPAIVDITNRELEADGI
                     PFREDVGHVRAWYSNASAGFDPVRDVTIAEIDGVPVAYADRSWVDTTLEPIWREYRMT
                     GAVLPEWQRKGIGTTLLRHNQEKQRELARTHETDHPRIFGSWTSSRAVGAIALLKANG
                     FDEARHFFEMTRPLNEPIPDVRAPEGLEIRPVREDEYRKIWKADVEAFKDHWGGFEDS
                     EEQYQRWLVRPDFDPTMWVVVYDGEKVAGASINGISKEENEQLGLKRGWLHSVFTTRE
                     YRRRGVARAAVAQSLKVLKERGMDTGILGVDATNPTGALRVYEGVGFSVAEHSTAWRK
                     PFEL"
     gene            complement(4385..5185)
                     /locus_tag="VIF08_07040"
     CDS             complement(4385..5185)
                     /locus_tag="VIF08_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8135783.1"
                     /translation="MTVLTREFRASFAFVERNFNLTKRYWGWEVAFMVYSVAGALAVT
                     MIGRDQQSARLILSLMIGATFWNYLSVVFSIIAEQISWERWEGTLEYTMMAPVRRVSQ
                     LLGSTVFAIMWGFIHTAVVLTVLVLFFGIDLSHADFLTALVLMLVGSLSIIGIGMMAA
                     TLPLIWVERGDQMVFVIQSLLLLISGVYYSVTILPAPMQVLAAFSPATYILDGVRQAL
                     IDGAPVTSMGGHLLPLAIMAVIFIPLGVWVFGWAERYAKRTGKLKRVG"
     gene            complement(5449..6348)
                     /locus_tag="VIF08_07045"
     CDS             complement(5449..6348)
                     /locus_tag="VIF08_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135784.1"
                     /translation="MTPPKTSQPILEPQPQELTSTAQAPVIALDIEHLTKRFEVGGRK
                     KKRKTVVAVNDISLRIERGEIYGLLGANGSGKSTFIRLLSTLLTVEHGRVAVFGHDIV
                     RDEMAVKRLINRVSVDAAFFKKLSPLENLMYAARLYDVPASTAKREIVRILGRLGIAE
                     SRLGRPLEQMSRGMQQKVAIARAFLTSPVVLLLDEPTTGLDPRSKLEVQSFIEEIRDL
                     HDATIVLTTHDLDEAERLCSRIGLLNDGRLVAEGTVNELKAGVGPQVGPDGAPRAATL
                     HDVFMAHTGRSLDDDVEESGDDD"
     gene            complement(6793..7539)
                     /locus_tag="VIF08_07050"
     CDS             complement(6793..7539)
                     /locus_tag="VIF08_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY8135785.1"
                     /translation="MTASLAAAITVSVGGLTSPQPAFAADPAPTQQSDAQSRRVNQRA
                     KIVRITESYLGAQYRFGAEGTEYVDCSGLVWRVYKEAGLEAKIGGARKGARAYYHWFK
                     RRGLASRTNPKPGDLVIWRKGKHIGIYVGNGYAISALNKKLDVRKHKVNWLGGFTAYL
                     HVRLNGDVPNTNDGSTSPDTLFKVTSDVDGLIVRDGPGTGHERVAMVDRGTVIRVTDK
                     AFGAQGRKWFEGKLPSGKVGWVLASRVTRI"
     gene            complement(7860..8084)
                     /locus_tag="VIF08_07055"
     CDS             complement(7860..8084)
                     /locus_tag="VIF08_07055"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY8135786.1"
                     /translation="MCRSIVRLRDHDQIVADEEIRAAALQFVRKISGYRKPSAANEEA
                     FNRAVDEIAHSSQHLLEAVVAGLPGHKHAA"
     gene            complement(8089..8436)
                     /locus_tag="VIF08_07060"
     CDS             complement(8089..8436)
                     /locus_tag="VIF08_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135787.1"
                     /translation="MATRVGRGLDGLAVSDLAALRLASQVLAHKAALAGLPRVAMFLT
                     FVEAEVAAEAAARGQRGQRGAVSADPWRTVPLTPTDRATIRAYLELLRDNERLSPAVR
                     DYVRRLLAAGRVS"
     gene            8462..9778
                     /locus_tag="VIF08_07065"
     CDS             8462..9778
                     /locus_tag="VIF08_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135788.1"
                     /translation="MRHTSAVDGWRRFGEPRFRNYHYLALAQGVVGVLAGFDVVIPWA
                     LAMGCPPFAAILLGVLPLAGGIAQLVVPRLLDRTGGNLRGLTILIAAVGEPRGLYFAA
                     LAALYAAGIVSGPVALVALAVLLAITSVLSSVTGANLLSWHSAVLPDQDRRLVVPRLL
                     AVSLAIGALLLLPMAVLLDSLVHAIGVYAYALPFVVSGLLGLVELWVLRRLRHPGVVL
                     VPPAAATADSEPTPELNRFFRSTTINALGMGITPSFSVLIISVLGMTAGFAMTTGAIG
                     TLTMVVAAAFFGDRLTRGSSSRMLRGSFGVRAMAMTAALLTWPLPMLAPVLIIAAAML
                     GSIGFAGGQLASNERLFRLVRGPQVVRHYARYLARTSGAMTIGQLFSAGVIAVSGPAA
                     PAFAGLYGASALIRVYAFRDARPTVAEVPASPEPVVEPLPAVQSAN"
     gene            complement(9861..10061)
                     /locus_tag="VIF08_07070"
     CDS             complement(9861..10061)
                     /locus_tag="VIF08_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135789.1"
                     /translation="MTTSSSSVWRRSVPKDAPPEPFKHLIDRYQHREQLVTCTCGWQG
                     SSLDSAADGWKAHLLEVRGPSR"
     gene            complement(10205..10621)
                     /locus_tag="VIF08_07075"
     CDS             complement(10205..10621)
                     /locus_tag="VIF08_07075"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY8135790.1"
                     /translation="MFKSTDALTPDEYIDRLEEPRRTEIRRLHELIRRTAPTLEPHLA
                     SGMLAYGPYQYRGKSSSGTWFRIGLASHKRYISLYVMAEDMVTGGYLAESYKDRLPRA
                     DIGKSCVRFKRAEDLDQATLAQLIHEAATLRPLAQA"
     gene            complement(10654..13662)
                     /locus_tag="VIF08_07080"
     CDS             complement(10654..13662)
                     /locus_tag="VIF08_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8135791.1"
                     /translation="MTSAARALESLTTGLPAQISTSAAPPGRVAILAYVPISGAAIGA
                     YLAFPDSPLGGLAFLVSSAVALGALVLGPLVHRPASTLPWRWLALGMAAFLVGNTLRF
                     LTSLGVGSQLSWLADSAYVGYVFLAIGLMSFIRVRRPRYRIARLIDAFAIGATVVTLT
                     SIPTLESALSRAATDVLLAGAVALLLEVLIVGGTAYLVIAGDGFRGRAQQVLFLGVIA
                     TAVADTLFQANGTSSGFSNTIMNTLWLTSYMLIGLTALAPSMRQLTEPHAQEPQLAAG
                     RAMTALLGAAAVLTLVNISSRLSGQVSMGVAEVVEGAILLLLFVRARELSGIETRRER
                     AIALLLANAADALVVVDAQGVVTLANPPAARMFGVSESESIGRPVVEFLDRVVPDERE
                     SLRRRFMLVLTKPAATDRSLIRLPASDRSYRTMDVTATNRLDDPAVRGVVLNFRDVTD
                     QVRANEQVARLGTAIEQAYDAVLLTDVDGTIEYANPAFERLTGYARDEIVGKNPRILK
                     SGDQTPAFYSAMWQTLASGKPWRADFVNVRKDGTRYRMAGVITPLHDETGRRTGYVGV
                     SRDLTRQYELERESRTMNRQRALIAETLRSMDPSGSPEEIGQTICGQVVQIDGISTSA
                     LVVFEADGTAIPYGIAAADGRSLPRRRLPRKRVDEVNGQASRGPWIERWEPKSWHPYN
                     ELLNDLGVRAIAYAPVRLNGDLAGYLLASSSDADAEALLATVLPSLVEFASISATILG
                     AKISERTQLGAARQRLTQVIERHQFFPVYQPLCETATERVVGYEALTRFDDGVAPDIR
                     FAQAYEAGVGEKLELAAIEAALQGAADFPQGMWLNINATPDVIMDGGGLQRLLRGYDR
                     DLVLEVTEHSEITDYRAFRQALVELGPRVRLAVDDAGAGFASLRHILELRPAFVKLDR
                     QVIMGIDDDEARQAMVAGLRHFALNTGCWLIAEGVETAAELATLKALDVRYVQGFLLG
                     RPVPTPHSDGPVGAPHAGGGDVRGVRRRPR"
     gene            13881..15878
                     /gene="dnaG"
                     /locus_tag="VIF08_07085"
     CDS             13881..15878
                     /gene="dnaG"
                     /locus_tag="VIF08_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY8135792.1"
                     /translation="MSGVSRNPREETATSSVGVAAEVKGKLNLVDLIGETVQLRKAGA
                     SFKGLCPFHNEKTPSFTVTPARETWKCFGCGRGGDVFNFVMERDSIDFPEALRRLAGR
                     AGVEISERTTREDARRKRLRDALESAISFYHRVLTESHHGKPALDYLRGRGFTDATIS
                     DHLLGYAVDAWDTLSKSLIEKRGFKEDELEAAGLVTRRQGRKGTYDRFRGRIIFPIRD
                     ASGNATGLGGRIIGSQQREANGRDGGPKYLNSPATPLFDKSRTLYLIDRAKGAIRKAG
                     LAVLVEGNTDALMAHQQGFENVVGTLGTALTPAQIELVTRYAPRIALAYDVDAAGQGA
                     ATFGATELSALVGEIERSAHKGRLTDVGVIRLPDQRDPDEVMRDDPAAWRKATEEPLP
                     IVEYLIDHFAKRHDPRTLSGRDKLVAAVMPTIRRVTNPTQRDGYLQLLARRSGVEERV
                     LLEELRRPEQAERVVGRPAEAHVGSKINLEAVLSSPDALDPQAVALLVKPAEFSLLRL
                     VLLRPYLRDRIDDPGAIEFVSTPAREIWTRLGDVPAAGFDRAAFAETLDPILAGVVRT
                     MYADPEPLPDDEDALHQALEQSLLTLRRNRLDEEIGAKEYDIREAEADGDRPAAEGLL
                     REVMDLKQRRLDLDRQRDTTTVLTQRRQRATAASAASGGNG"
     gene            15882..17522
                     /gene="rpoD"
                     /locus_tag="VIF08_07090"
     CDS             15882..17522
                     /gene="rpoD"
                     /locus_tag="VIF08_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY8135793.1"
                     /translation="MDSDKLLVASVLERGKRRRDDDLNDQKDEEDEAPSNGMSAAAEL
                     GVEVQIDGDGGQTPWEEPPAKLDAKDADEPTAEELDAIAADMIGIDDPVRMYLKEIGK
                     VPLLNAEEEVVLAKAIELGEQIIEEPHKAVLSLWEWTRNQTETKSRDKHPQHRLPYKE
                     ETEKIVTGAFKAAQKDGLYQHTPDFHFVKAGKAVEGDIAKATLKEASHKVKAYNEDAI
                     LANFTELVDFSFRAAHSTDAETHDNSAIRAVYEWSRGVAWEALRRWIYNGKDAELMAE
                     MGWTPEQDMSLKPRDRRGVLVRYGKEGREQLTSANLRLVVSIAKKYIGRGMSFLDLIQ
                     EGNIGLIRAVEKFDYEKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINRLI
                     RVSRGLLQELGREPTVEEIAEAMSRGQEVVVTPDKVREIIKVSQEPVSLETPIGEEED
                     SHLGDFIEDRAALAPAEAASHQLLKEQVEAVLDSLSGRERRVLQLRFGLEDGRARTRE
                     EVGKEFNVTRERIRQIEAKALRKLRHPSRSRKLKDYLE"
     gene            17749..18402
                     /locus_tag="VIF08_07095"
     CDS             17749..18402
                     /locus_tag="VIF08_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8135794.1"
                     /translation="MVAKQRAVDRGTARGRTLVSTIGGEIRLARQRAGLSLEAVARAA
                     GVSGVEVSRIERARAEWVSLLTLARLCTIVGLDLTARAYPGANPLRDARHNRLLMLLH
                     GHINPALGWALEVPLPNPGDQRAWDAMIRGDGWRYGVECEMNPIDGQAALRRLTLKQK
                     DGLVDGVTLLLPDTRQARLFRREFVEQLAAQFPVRSIDALRALAAGKNPGGSAIITP"
     gene            18399..18851
                     /locus_tag="VIF08_07100"
     CDS             18399..18851
                     /locus_tag="VIF08_07100"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEY8135795.1"
                     /translation="MKFKTTLKQAEGSTATGIEIPDEVLAALGAGKKPPVKLSVNGYR
                     YRSTVATVSGAYMVGFSADHRAASGLKGGDPIEVQIELDTEPRTVELPADFQAALDAD
                     TQARATFEKLSNSLKGYHVSQVTGTKNPETRRRRIEKSIATLRAGKPR"
     gene            complement(18848..19414)
                     /locus_tag="VIF08_07105"
     CDS             complement(18848..19414)
                     /locus_tag="VIF08_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY8135796.1"
                     /translation="MPVSVQASRQVAEPMEILRRTPDVLRALLAGLPDTWTDTPDTPD
                     GWRPRDVVGHLITGELSDWLARTKRILEDGTSREFDRFDRVAMLERDRGASLDELLDR
                     FAALRAQNLAEFAALVSDDDLGKLGLHPSLGEVTLDNLLNTWAVHDLDHIGQIFAGLA
                     GSHDADVGPWKVYLGMLLRRTDPSAVPG"
     gene            complement(19414..19995)
                     /locus_tag="VIF08_07110"
     CDS             complement(19414..19995)
                     /locus_tag="VIF08_07110"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEY8135797.1"
                     /translation="MSQRRPRSWQEMREQEIKWLIERTGDGLETWNARVRESGKTDEA
                     GLRAWLSEKGVTGYPAMLLVMERFGYPEYLQTSAEDLIDNQYAGRPATRPIYDALLAL
                     LPDVGEIEVQARKTYVAFLTPKRTFAALVPATKTRSDLGLRLPATQPATGTLEAAKNF
                     PQSSVTHKIGLASADDIDDEVVGWIKRSYAENI"
     gene            complement(19992..20585)
                     /locus_tag="VIF08_07115"
     CDS             complement(19992..20585)
                     /locus_tag="VIF08_07115"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8135798.1"
                     /translation="MNDQGARPVPILRGEHVYLRPAERADVPSFVRWLNDAETTSFLN
                     MRAPMSVAMEEKWFEGMVERQGKDHYHYVICLLKNDQPIGSLSLFAVDYINGNAGIGI
                     SIGEKTLWGKGYGTDAMNALVDFGFGMLRLERMWLEVYVFNKRARRSYDKSGFVLEGV
                     ERHGVFKQGRYLDVEMMSILRDEWAALPRKKAWDLNS"
     gene            complement(20582..>21340)
                     /locus_tag="VIF08_07120"
     CDS             complement(20582..>21340)
                     /locus_tag="VIF08_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8135799.1"
                     /translation="NKPGAGRAAYDAGHILKAIFVDLDDDLSDLDGYGAPGRHPLPTA
                     DAFRERMEELGIGSDSLVVAYDDVGGAIAARLWWMLDNLGHRGGVAVLDGGIKAWVAQ
                     GMALSTDEPHYDRARLELADHWTNVIAREELAVRLGSVTLLDARARERYRGEVEPIDP
                     IPGHIPTAISAPTMDNVDGAGYFLDQPALIARFAGLVADDRPTVNYCGSGTNACHNAL
                     AMRLAGLPDPILYVGSYSDWSRSGMATEPSREGK"
BASE COUNT         3599 a         7181 c         7099 g         3461 t
ORIGIN      
        1 gacgtccgta tcgccggcaa agtagacccg tcggccgtcc tccaggccca ccacgaagcc
       61 gaccggctca cccaggtaca gcggcccgga gccgagcccg ctgcggctgg ccatgggccc
      121 aagggccggc cagtcgcccg cggagtgatc ggcgtggacc atgctcacac tcaagccgcg
      181 cgtctggacg gtaccgccct tgttcatgcc gatgacctcg acgccagccc cctcgagctc
      241 ggtctccagc cagagcgaca gctcgtggat gcacggccag gccgggttgg tgcggcgggc
      301 gatggtgagc gcgttggcgt cgctgacgtg gcgtggctcg gagcccatgt gatcgaaatg
      361 gccgtgcgag acgagcatca cgtcgcagcg ctccaggtcg gccgcggcgc gcgttgaggt
      421 gggattgccg aaccacgggt cgaatagcac gaccttgtcc cccggcgtgc gcagctccac
      481 gcacgagtgg ccgaaccacg tgaagcgcgc actcatcttc tggaccctct ccaatgctca
      541 gtgccgaatg ccgtcattct ggcgccgcgt ggtcaaggtg caagagtcgg cgcgcgtaac
      601 atcacggcca gcgtcaccct gaaggaggta gaggtggatt tcctgccggc gatctttctg
      661 gtcctgttga tcatctgcgc gatcggcgcg ctgctggtca ctgcggtgcc cgatcagcgg
      721 gctcaccagc tgcagcttca gagccgtcgc ttcggcaacg atgccggcga caagggagag
      781 gtccaagtgc gctacccgtt ccggcgcacc ctcattgccg gcgccttcgc gtttcttgtg
      841 ctgtggatcg tgtccctggg cgtggtcatc gtgcccgccg gcttcgtcgg tgtcgtgcgc
      901 ttcctcggcc aggttcagcc caacgtgctc tatccgggcg cgagctgggt cgtgccgttc
      961 gtcaacaccg ttgaggaggt cgacacccgc gttcagccgc acaacttcca gcagctgacc
     1021 gctgcaaccg cggagaacct ccaggtgacc gtcaccggca ccatgaacta ccaccttgcc
     1081 gcggccttcg cggggcagct gatccagaac gtcggcaccg acttcgcgag caagatcatc
     1141 gatcccgcct tcagcgacta catcaagcag gtcaccccgg cctactcggc cgacagcaac
     1201 agcccgaact tcatcctcaa caagcgcgac gagatccggc agctcaccaa gcaacagctg
     1261 ggcgacaacc tagcccgcta cggcatcatc gtcgacgaca tctacatcgt tgatatcggc
     1321 tacccgcagg cctacaacga cgccatcacg cagaaacagg tcgagcagca gaacgtccag
     1381 cgcgagcagc agatcctcga gcagcggcgg atccaggccc agcaggtcgt agtcacggcc
     1441 cagggagcgg ccgacgcgct acgcgagacc gctgctggtc aggcggatgc caatgcgtcc
     1501 atcaacagct cgctgaccga cgagctcatc cgctggcaag ccatccagaa gctgaatccg
     1561 aatgtgcaga tcatgctgat cccgtcggac aacaacttca tactgccgct gccctcgctc
     1621 gaaccggtga ctccgacgcc agtcccctga aactcctgca gcgcgtcgtc gggccgatcg
     1681 cgaccaatct ctacgtgctc ggcgatgagc cgagcggcga ggcgatcgcg attgacacgg
     1741 cgacgccgtg cgtcgaatgg ttgactgcca cgctggccga acgcggctgg cgcctgaagc
     1801 tcgtggtcag cacgcatcgc cactgggatc acatcggcga caacgcggcg gtgagcgcgg
     1861 cgaccggggc aggaattgcc gcgcacatcg ctgatcgcca cggcctcgag catccgaccg
     1921 gccacctcgc gccatttcca atcccaccaa gcgtgccggc gctcgacctg gcagaagggt
     1981 cgaccatccg cttcggcgag atcacgctca cgatcctgca cacaccgggc cacaccgaag
     2041 ggtccgtctg cctgatggcc gattcggagg ggttgctctt caccggcgac acgttgtttg
     2101 ccggcagctg gggccggacc gatctgcccg gtgggtcgga ggaggcgatg gtcgattcgc
     2161 tcggccgatt ggcgggcctg ggcgatgacc tgaaggtgtt gcccggccac ggcagccaga
     2221 cgactctggc ggccgaaaag ccctggttgg agcaggtcat ccgaacgcgc ctgctgccct
     2281 tctaggcgtc ggcgccgtac ttggtgcgca tgtgagtgcg caccgacttg tggatcgcgg
     2341 tccagcgctt gcggtgcgcg gccctggcga ccggatcgac gcgcgccgcc agcgcggctg
     2401 actcgcgcgc gagcttgcgc tggctttcga gacgtgccgc gggaagcgag ccgtcggcga
     2461 tcgcctgctg gacggcacag cccggctcac cggtatgcgt gcagtcaccg aatcggcagc
     2521 cggcggcgtg ctcctcgatg tcggcgaatg cgtcggccag accttcttcg acctcccaca
     2581 tgccgaccga gcgcatgcct ggcgagtcga tgagcagtgc cccggacggc atggcgagga
     2641 gctcgcgcat ggtcgtggtg tgccggccgc gctgatcgtc ggcgcgcact tcgcctgtct
     2701 gctgtcgttc gtacccgagc agcgcgttga cgatggtcga cttgccgaca cccgatgagc
     2761 cgagcagggc gaccgtcttg cccgcttcga gcagtggctc gagtgactcg atgccggtcc
     2821 tgtcgcgcgc gctgagggcg cgcacgctga cacctggcgc gaccaggctc acgtctgcga
     2881 tgcgaccggc gacgtcgtcg cacaggtctg ccttgttgag cacgatgacc ggctcggcgc
     2941 cgctcgacca ggcgagggcc aggtagcgct ccagtcggcg caggttgaag tcgtgatcga
     3001 gagcagtgac gagcagcacg agatcgacgt tcgccgcgac cacttgagca gccgcgatgt
     3061 caccgcgtgc cggacgcgcg aagcgggtca ttcgcggcaa aacggcatgg atcaccgccg
     3121 tgtccgaacc cggcgcgacc tcgagcgcga cccagtcacc gaccgcaggg aagtcctccg
     3181 agcccgcggc gtcgaagcgg aaccggccgg agaccgagcc atcaacgtcg ccaccctcag
     3241 ccgccacgac gtagccaccg cgatgctcac tgagcacgcg gccgggttgc agcgcggcat
     3301 cgaggtgggg ctggaagagc gtcgcttcgc gctctgtcca accccaccgc tcgagaaccg
     3361 tcattacagc tcgaagggct tgcgccaggc tgtcgagtgc tccgcgacgg agaacccgac
     3421 gccctcgtag acacgcaacg cgcccgtggg gttggtggca tcgacaccga ggatcccggt
     3481 gtccatgccg cgctccttga gcaccttgag tgactgcgcg acggctgctc gcgccacgcc
     3541 acgacggcgg tactcgcgtg tggtgaagac gctgtgcagc cagccgcgct tgaggccgag
     3601 ctgctcgttc tcctccttgg agatcccgtt gatcgacgcc ccggcaacct tctcgccgtc
     3661 atagaccacc acccacatcg ttgggtcgaa gtcgggccgg acaagccagc gctggtattg
     3721 ctcctcggag tcctcgaagc cgccccagtg atccttgaac gcctcgacgt cggccttcca
     3781 gatcttgcgg tactcgtcct cgcgcaccgg tcggatctcc agcccttcgg gggcacggac
     3841 atcggggatc ggctcgttga gcggacgcgt catttcgaag aagtgacgcg cctcgtcgaa
     3901 gccgttcgcc ttcagcagcg cgatggcacc gacagcacgg gacgatgtcc agctgccgaa
     3961 gatgcgcggg tggtcggtct cgtgcgtccg cgcaagctca cgctgcttct cctggttgtg
     4021 ccgcagaagc gtcgtcccga tgcccttgcg ttgccactcg ggcagcacgg caccggtcat
     4081 gcggtactcg cgccagatcg gctcgagggt cgtgtcgacc catgagcggt cggcgtaagc
     4141 gactggcacg ccatcgatct cagcgatggt gacgtcgcga accgggtcga aacccgcgct
     4201 ggcattggaa taccaggcgc ggacgtgacc cacgtcctcg cggaacggga tgccgtccgc
     4261 ttcgagctcg cggttggtga tgtcgacgat ggccggtaga tccgcctcgc cggcatatgg
     4321 acggagcgtc agcccgggca ggccggcgcg ccgctcagta gtggcagttt tctcgttcat
     4381 ttctctaacc cactctcttg agcttgccgg tccgcttggc atagcgctcg gcccagccga
     4441 acacccacac gccgagcggg atgaatatca cggccatgat cgccaggggc aggagatggc
     4501 cgcccattga ggtgactgga gcgccgtcga tgagcgcctg gcggacgcca tcgaggatgt
     4561 acgtcgccgg tgagaacgcc gccaggacct gcatcggcgc aggcaggatc gtgacgctgt
     4621 agtagacacc gctgatcagc agcagcagcg actggatgac gaagaccatc tggtcaccgc
     4681 gctcgaccca gatgagcggc agcgtcgccg ccatcatgcc gatgccgatg atcgacagcg
     4741 agccgacgag catcaacacc agcgccgtca ggaagtcagc atgcgacagg tcgatgccaa
     4801 agaacagcac gagcaccgtc agcacgacgg ccgtgtggat gaagccccac atgatcgcga
     4861 acacggtcga gccgaggagc tgcgagacgc gccgtaccgg ggccatcatc gtgtactcga
     4921 gcgtgccttc ccagcgctcc cagctgatct gctcggcgat gatcgagaac accacgctca
     4981 ggtagttcca gaacgtcgcg ccgatcatca ggctcaggat caggcgcgcg ctctgctggt
     5041 cgcggccgat catggtgacg gcgagcgcgc ctgcgactga gtagaccatg aaagccactt
     5101 cccagcccca gtagcgcttg gtcaggttga agttgcgctc gacgaaggcg aatgaggcgc
     5161 ggaattcgcg agtgaggacg gtcatcggac tgttccttag ctgatggagg tgcgggcccg
     5221 cccctacagg gagcgggccc gtggacgtgc tcgttcggcg gccaggcggc tgccgatccg
     5281 aaccatcgat cggccgacgg cgcggcggat tcgccgctcc ggctgcagcg cggcgacgag
     5341 tcgccgctgg cgagcagcct tgcgctcttc gcgctgccgc tgccgagcga tgtcgatgtg
     5401 catgggatgg atcaacatct ggaaatcacc tcctttcgtt caagtttgtt agtcgtcgtc
     5461 gccgctctcc tcgacgtcgt cgtcgagcga gcgaccggta tgagccatga acacgtcgtg
     5521 cagcgtggct gcgcgaggcg cgccgtcggg tcccacctgg ggcccaacgc cggccttgag
     5581 ctcgttcacg gtgccctcgg cgaccagtcg gccgtcgttg agcagtccga tgcgcgagca
     5641 cagccgctcg gcctcatcga ggtcgtgcgt cgtcaggacg atcgtcgcgt cgtggagatc
     5701 gcggatctcc tcgatgaacg attggacctc gagcttcgag cgtggatcga gaccggtagt
     5761 cggctcgtcg agcagtagca caaccgggct tgtcaggaag gcgcgcgcga tggcgacctt
     5821 ctgctgcatg ccgcggctca tctgctcgag cggccggccc aggcgcgact cggcaatgcc
     5881 gagacggccc aggatgcgca cgatctcgcg cttcgccgtc gatgccggga cgtcgtacag
     5941 ccgggcggca tacatcaggt tctcgagcgg cgacagcttc ttgaagaaag ccgcgtcgac
     6001 actgacccga tttatcagcc gcttgactgc catctcgtcg cggacgatgt cgtggccgaa
     6061 gaccgcgaca cgtccgtgct ccaccgtcag tagcgtgctc agcaacctga tgaaggtcga
     6121 cttgccgctg ccgttggcgc cgagcagtcc atagatctcg ccacgctcga tgcgcagcga
     6181 gatgtcgttg actgcgacga cggtcttgcg cttcttcttg cgcccaccga cctcgaagcg
     6241 cttggtcagg tgctcgatat cgagcgcgat gaccggcgct tgagccgttg acgtcagctc
     6301 ttgaggctgc ggctcgagga tcggttggct ggttttcggt ggcgtcatgg tgatcgccat
     6361 ggctccttgc tcctgtggtg gcgttgctgc caaggacaag ggccccgatg cgtggcgcgg
     6421 gagaggcggc tcgcgatgcc tgctgcgcag ggcatcaaaa aagccggggg cccgatctgg
     6481 cctccggctt ctggttcagc ggtcgttccg cttactcaga tcagacaggc acagatctcc
     6541 cgcgctccga aacgccgaag ggcgcgtcgg accgcagcgc ggtggctgcg gcggtagggc
     6601 ggttgatgtt caacattcgg agatcggctc ctgttaaggc tggctcaggc gcgagcgcga
     6661 ttgcgcagga taggcgggtt tcgcgcgcgc tgtcaaatcg atattgcaaa acaaggcgag
     6721 aaacaaagac agcgagaaac aaagaagcgg ccgcgcgaga ggcatcgcgc ggccgcagga
     6781 ttacgccggg attcagatcc tggtgacgcg cgaggccagc acccagccga ccttgccgct
     6841 gggcagcttg ccctcgaacc acttccgacc ctgggcaccg aacgccttgt ccgtgacacg
     6901 gatgaccgtg ccgcggtcga ccatcgcgac gcgctcgtgg cccgtgcccg ggccgtcgcg
     6961 cacgatcagg ccgtccacgt ccgaggtgac cttgaagagg gtgtccgggg acgtggaccc
     7021 atcgttagtg ttgggcacgt cgccgttcag ccggacgtgc aggtaggcgg tgaagccgcc
     7081 caaccaattg accttgtgct tgcgcacgtc gagtttcttg ttcagggcgc tgatcgcata
     7141 gccgttgccg acgtagatgc cgatgtgctt gcccttgcgc cagatcacaa gatcacccgg
     7201 cttgggattc gtgcggctgg cgaggccgcg ccgcttgaac cagtggtagt aggcacgcgc
     7261 ccccttgcgg gcgccgccga tcttggcctc gagaccggct tccttgtaga cccgccaaac
     7321 caggcccgag cagtcgacgt attcggtgcc ctcggcgccg aacctgtact gggcgcccaa
     7381 gtagctctcg gtgatgcgca cgatcttggc gcgctgattc acccgccgcg actgagcgtc
     7441 ggactgctgg gtcggcgcgg gatcggcggc aaacgccggc tgcggactgg tcaggccgcc
     7501 gacggagacg gtgattgccg cggccaacga ggcggtcacg gcgaggcgag tgacacggct
     7561 agcgaaggac atagaggacc ccctctgggg cggcgcgcgc aaacacggcc gcgtgactta
     7621 cgaccgcgca gcacgaaacg cgctaggacg tcaggcgagc cactcacggc gccgaagctg
     7681 gggcaacggc aatgtggcac aaccaggtcc gtgacgagcg gtcttggttc ctatccccgc
     7741 cacgacaacg gggtcgggcc ggaggatcgc acaccaagca acgaaagcgc aaccccgggc
     7801 tggcgaagcg ttattcggcg ctgggtgacc gcattccaaa tggggcagtc ggcccaggtc
     7861 taggcggcgt gcttgtgtcc gggcaaaccg gcgacgacgg cctccagcag gtgctgcgac
     7921 gagtgggcga tctcgtccac ggcacggttg aacgcctctt cgttggctgc cgacggtttg
     7981 cgatagccgc tgatcttgcg cacgaactgc agcgcggccg cccggatctc ctcgtcagcc
     8041 acgatctggt cgtggtccct caatcgaacg atgcttcggc acatgtcctc aagatacgcg
     8101 accggccgcg agaaggcgcc tcacgtagtc acgcacggca ggggagagtc gctcgttgtc
     8161 gcgcagcagc tcgagatacg cccggatcgt cgcgcggtcg gtaggtgtca gcggcacggt
     8221 ccgccaggga tcggctgaga ctgcgccgcg ctggccgcgc tggccacgcg cggcagcctc
     8281 cgcggccacc tccgcctcga cgaacgtgag gaacatggcg acgcgcggca ggcccgccag
     8341 cgcggccttg tgggcgagca cctggctcgc caggcgcagc gccgcgaggt cgcttaccgc
     8401 caagccgtcg agcccgcgtc cgaccctcgt cgccacgtgg ccactgtatc cttcgagtgc
     8461 gttgcgtcac acctcggcgg tcgacggctg gcgccgattc ggcgagccgc gtttccggaa
     8521 ctaccactac ctggccctgg ctcagggcgt cgtcggcgtg ctcgccggct tcgacgtcgt
     8581 gatcccgtgg gcgctcgcga tgggctgccc gcctttcgcc gccatcctgc tcggcgtgct
     8641 gccgctcgcc ggcggcattg cccagctcgt cgtgccgcgg ctgctcgacc gcaccggcgg
     8701 caacctgcgc ggcctgacga tcctcatagc ggccgtgggc gagccgcgcg gcctgtactt
     8761 cgcggctctt gcggcgctct acgccgccgg catcgtcagc gggccggtcg ccctcgtggc
     8821 cctggccgtt ctgctcgcca tcaccagcgt gctcagctcc gtcaccgggg caaacctgct
     8881 gtcgtggcac tcggcggtgc tgcccgacca ggaccggcgc ctggtcgtcc cgcggctgct
     8941 ggcggtgtcg ttggcaatcg gtgccctgct gctgctgccc atggcggtgc tgctcgactc
     9001 gctggtccac gccatcggcg tttacgccta cgcgctgccg tttgtcgtct cgggcctcct
     9061 cggcctggtc gagctgtggg tgctgcggcg cctccgccac ccaggtgttg tcctcgtccc
     9121 gccagccgca gcgacggccg atagcgagcc cacgcccgag ctcaatcgat tcttccgatc
     9181 caccaccatc aacgcactcg gcatgggcat cacgccatcg ttctcggtgc tgatcatctc
     9241 ggtcctgggc atgaccgccg gtttcgccat gacgacgggc gcgatcggca cgttgaccat
     9301 ggtcgtggcc gcggccttct tcggcgaccg cctgacccgg ggctcgtcat cgcgcatgct
     9361 gcgcggctcc ttcggcgtac gtgccatggc catgaccgcg gcactgctga cctggcctct
     9421 gccgatgctc gcgccggtgc tgatcatcgc ggccgcgatg ctcggctcga tcggcttcgc
     9481 cggcggccag ctggcgtcca atgagcggct gttcaggctg gtccgcggcc cccaggtcgt
     9541 gcgccactac gcgcgctacc tggcgcgcac gtcgggcgcc atgacgatcg gtcagctctt
     9601 cagcgccggc gtgattgccg tttccggtcc cgctgcgccg gccttcgccg gcctgtacgg
     9661 cgcgtcggcg ctcatccgcg tctatgcgtt cagggatgcc cgccccacgg tcgccgaagt
     9721 cccggccagc ccggagccgg tggttgaacc actgccggcc gtccagtcgg ccaactgacg
     9781 gcccatcgcc aagcgcgggc gaaatgaaaa agcgcccgtt cggtgagaac gggcgccctg
     9841 gggtcgtgtt tgagacgcga ctagcgcgac gggccgcgca cctcgagcag gtgagccttc
     9901 cagccgtcag cggccgagtc aaggctgctg ccctgccagc cgcacgtgca cgtgaccagc
     9961 tgctcgcgat gctgatagcg gtcgatcagg tgcttgaacg gctcaggtgg cgcgtccttg
    10021 ggaacggagc ggcgccagac ggatgagctc gacgtagtca cgcgctactg acctctctta
    10081 catgcctctt cggtccgccg gctgcacgtg acgccaccaa tagcgggggt ggggtaacga
    10141 ccgggaccga ccctcgggga gccctaattc tagccgattt tgcgcttccc ggccagcgtc
    10201 gcggtcaggc ctgggcgagc ggccggagcg tggcggcctc gtggatcagc tgtgccaggg
    10261 tggcttgatc gaggtcctcg gcgcgcttga aacgcacgca gctcttgccg atatcggccc
    10321 tgggcaagcg gtctttgtag ctctcggcca gatagccccc ggtgaccatg tcctccgcca
    10381 tcacgtagag cgagatgtag cgcttgtggc tcgccaggcc aatccgaaac caggtgccgg
    10441 agctgctctt gccgcggtat tggtacgggc cgtaggcgag catccccgaa gccaggtgcg
    10501 gctcgagtgt gggggcggtg cggcggatca gctcgtgcaa ccgccgaatc tcggtgcggc
    10561 gtggctcctc gaggcggtcg atgtactcgt cgggagtcag ggcgtcggtc gacttgaaca
    10621 ccgcccgatg atcggcggca ctgtcaaggc ccgtcagcgg gggcgtcgcc ggacgccgcg
    10681 cacatcgccg ccgccggcat gcggcgctcc taccgggcca tcgctgtgtg gtgtgggcac
    10741 gggtcgcccc agcaggaaac cctggacgta gcgaacgtcg agcgccttca gcgtggccag
    10801 ctccgcagcg gtctcgacgc cttcggcaat cagccagcag ccggtgttca gggcaaagtg
    10861 gcgcagcccg gcgaccattg cctggcgcgc ctcgtcgtcg tcaatgccca tgatcacctg
    10921 gcgatcgagc ttcacgaacg cggggcgcag ctccaggatg tggcgcaggc tcgcgaagcc
    10981 ggcacccgca tcgtcgactg cgaggcggac ccgcggacca agctccacca gggcctgacg
    11041 gaaggcccgg tagtcggtga tctccgagtg ctcggtgacc tcgaggacga ggtcccggtc
    11101 atagccgcgg agaaggcgct gcagcccgcc accatccatg atcacgtcgg gcgtcgcgtt
    11161 gatgttcagc cacatgccct ggggaaagtc ggccgcgccc tgcagcgccg cttcgatcgc
    11221 ggccagctcg agcttctcac cgacgcctgc ttcgtacgcc tgggcgaagc gaatatccgg
    11281 cgcgacgcca tcgtcgaacc gggtcagcgc ttcgtagccg acgacccgct cggtcgcggt
    11341 ttcgcacagc ggctggtaca ccgggaagaa ttggtggcgc tcgatgacct gggtcagccg
    11401 ctggcgggcg gctcccagct gggtgcgctc ggagatcttg gcgccgagga tggtggctga
    11461 gatcgaggcg aattccacca gcgacggcag gacggtcgcg aggagtgcct cggcgtcggc
    11521 gtcggacgag ctcgcgagca ggtaaccggc cagatcgcca ttcaggcgca ccggggcgta
    11581 tgcgatcgcc cggacgccca ggtcgttcag cagctcgttg taggggtgcc acgactttgg
    11641 ctcccagcgt tcgatccacg gtccgcgcga cgcctgcccg ttcacctcat cgacgcgctt
    11701 tcgcggcaac cgtcgccggg gcagggagcg cccgtcggcg gccgcgatgc catacggaat
    11761 cgcggtcccg tccgcttcga agaccaccag tgccgatgtc gagatgccat cgatctggac
    11821 gacctggccg cagatcgtct ggccgatctc ctcgggtgat ccggatggat ccatggaccg
    11881 cagcgtttcg gcgatcaggg ctcgttggcg gttcatcgtg cgtgactctc gttcgagctc
    11941 gtactgcctg gtcaggtcgc gcgacacacc cacatagccg gtcctgcgcc cggtctcatc
    12001 gtggagcggc gtgattacgc cagccatgcg gtagcgcgtg ccgtccttgc gcacgttcac
    12061 gaagtcggcg cgccatggct tgccggacgc cagcgtctgc cacatggccg agtagaacgc
    12121 cggcgtctga tcaccgctct tgaggatgcg cgggttcttg cccacgatct cgtcgcgggc
    12181 gtacccggta aggcgctcga atgcaggatt cgcgtactcg atcgtgccat cgacgtcggt
    12241 cagcaacacg gcgtcatacg cctgttcgat cgccgttccc aggcgggcga cctgttcatt
    12301 ggcccgaacc tggtcggtga catcgcggaa gttcagcacg actccgcgaa cggcgggatc
    12361 atcgaggcga tttgtggccg tgacatccat ggttcggtaa ctgcgatcgg aggccggcag
    12421 ccggataaga gacctgtccg tcgcggccgg cttggtcagc acgagcatga agcgtcggcg
    12481 caaggattcg cgctcatcgg ggacgactcg gtccaggaat tcgaccaccg gccgcccaat
    12541 tgattcgctc tccgatacgc cgaacattcg ggcggcgggg ggattggcca gcgtgaccac
    12601 cccctgggcg tccaccacca ccaaagcgtc ggcggcgttt gccagcagga gcgctattgc
    12661 tcgctcacgg cgcgtctcaa tgccggatag ctcacgcgcg cgcacgaaga gcagcagcag
    12721 gatggcgccc tcgacgacct cggccacccc catcgagacc tggccgctta ggcgtgatga
    12781 gatgttgacc agcgtcagca cggcggccgc gccgagcagc gccgtcatgg cccgtccggc
    12841 ggcgagctgg ggttcctgag cgtgcggctc cgtgagctgg cgcatcgagg gtgcgagcgc
    12901 cgtcaggccg atgagcatgt agctcgtcag ccacagcgtg ttcatgatcg tgttgctgaa
    12961 tccggacgac gttccattcg cctggaagag cgtgtcggcg acagcggtcg cgatcacgcc
    13021 caggaacagt acctgctgtg ctcggccacg gaacccgtct ccggcaataa cgaggtaggc
    13081 ggtcccgcca acgatgagta cctcgagcag cagcgcgacc gcgccggcca gaaggacgtc
    13141 agtggccgcg cggctgagcg cgctctcgag cgttgggata gacgtcaggg ttaccacggt
    13201 cgcgccgatc gcgaaggcgt cgatgagccg agcaattcgg tagcgcggac ggcgtacgcg
    13261 aatgaagctc ataaggccaa tcgccaggaa gacatagccg acgtacgcgc tgtcggccag
    13321 ccacgacaac tgggatccaa cgcccagact ggtgaggaag cgcagcgtat tgccgaccag
    13381 gaaggcggcc atgcccaggg ccagccatcg ccagggcagt gtgctggcag gccggtgcac
    13441 gaggggccca aggacgaggg cgccaagcgc cactgcggag ctcacaagga aagccaggcc
    13501 gcccagcgga ctgtcgggga atgccaggta ggccccgatc gcggcaccac tgatgggcac
    13561 gtaagcaagg atcgccacgc gaccgggtgg agccgcgctc gtactgatct gtgccggcaa
    13621 gccggttgtg agcgattcaa gggcgcgcgc ggcgctggtc atgtcgttct ccgccgagcg
    13681 tctcgtgcgc ccggagatgc gatcgtccgc ccgcccccga tcacaaggca gatgccattc
    13741 ggcccctgaa atccatctga aggtccgggg gggcgacgtg tcaatactcc cggtgggtat
    13801 tgacacggct tgtcaagcgg ccgtgtcaag gggtagaatt gcctgcggaa gccttgtcaa
    13861 gacttgacaa ggcccttcgt gtgtccggcg tatcgcggaa ccctcgggag gaaacagcca
    13921 cgtcgtctgt cggcgtcgcc gcggaagtca aggggaagct caatctcgtc gatctgatcg
    13981 gcgagaccgt ccagctgcgc aaggctggcg cgagcttcaa gggactctgc ccctttcata
    14041 atgagaagac gccgtctttc acggtcacgc ccgcgcgcga gacctggaag tgcttcggct
    14101 gcggccgcgg cggggacgtc ttcaacttcg tcatggagcg cgactccatc gacttccccg
    14161 aggcgctgcg ccgtctcgcc ggccgcgcgg gagtggaaat cagcgagcgc acgacgcgcg
    14221 aggacgcgcg gcgcaagcgg ctgcgcgatg ccctcgaatc cgctatctcc ttctaccacc
    14281 gcgtcctcac ggagagccac cacggcaagc ccgcgctcga ctacctgcgc ggccgcggct
    14341 tcaccgacgc caccatcagt gaccacctgc tgggctatgc ggtcgatgcc tgggacacgc
    14401 tcagcaagtc gctcatcgag aagcgcggct tcaaggagga cgagctggag gcagcgggcc
    14461 tcgtcacgcg ccgccagggc cgcaagggca cctacgaccg cttccgcggc cggatcatct
    14521 ttcccatccg tgacgcgtca ggcaacgcga ccggcctcgg cggccggatc atcggcagcc
    14581 agcagaggga ggccaacggc cgcgatggcg ggcccaaata cctcaattcg ccggccacgc
    14641 cgctgttcga caagagccgc acgctttatc tcatcgaccg cgccaagggc gccatcagga
    14701 aggctggtct ggccgtgctg gtcgagggca acaccgacgc cctcatggcc caccagcagg
    14761 gcttcgagaa cgtcgtcggc acgctcggca cggcgctgac gcccgcccag atcgagctcg
    14821 tgacgcgcta cgcgccgcgc atcgccctgg catacgacgt ggatgccgcg ggccagggcg
    14881 cggccacgtt cggcgcgacc gaattgagtg cgctcgtcgg tgagatcgag cgcagcgcgc
    14941 acaagggccg cttgaccgac gtgggcgtca tacgcctgcc cgaccagcgc gaccccgacg
    15001 aggtcatgcg cgacgacccg gcggcgtggc gcaaggcgac cgaggagccg ctgcccatcg
    15061 tcgagtacct gatcgatcat ttcgccaagc gccacgatcc gcgcaccctg tccggccgcg
    15121 acaagctcgt cgccgccgtc atgccgacca tccgccgggt gaccaatccc acccagagag
    15181 acggttacct gcagctcctg gcgcggcgca gtggagtcga ggagcgggtg ttgcttgaag
    15241 agctccgtcg gcccgagcag gcagagcggg tagtcggtcg gccagctgag gcgcacgttg
    15301 gctcaaagat caatctcgag gccgtcctgt cgagccccga cgcgctcgac ccccaggccg
    15361 tcgctcttct tgtgaagccg gccgagttca gcctgttgcg gctcgtgctc ctccggccgt
    15421 acctgcgcga ccgcatcgac gatcccggtg ccatcgagtt cgtcagcacc ccggcgcgcg
    15481 agatctggac gcgcctcggg gatgtgcctg cggcgggttt tgaccgcgcg gcgttcgcgg
    15541 agacgctcga cccaatcctg gccggcgtcg tacgaaccat gtacgccgat ccggagccgc
    15601 tgcccgacga cgaggacgcg ctccaccagg cgctcgagca gagcctgctg acactgcgtc
    15661 gcaaccggct cgacgaggag atcggtgcca aggaatacga catccgtgag gcggaggccg
    15721 acggcgaccg gcccgcggcc gaggggctgc tgcgcgaggt catggacctc aagcaacgcc
    15781 gcctcgatct cgaccgtcaa cgcgacacga caacagttct cacccaaaga cgacaacgag
    15841 ccacggccgc atcggccgcc agcggaggta acggataacc aatggatagc gacaagcttc
    15901 ttgtggcgag cgtcctcgag cgcggcaagc gccgccgcga cgacgacctc aacgaccaga
    15961 aggatgaaga ggacgaagcg ccgagcaacg ggatgagcgc cgcggccgag ctgggcgtcg
    16021 aggtgcagat cgacggcgac ggcggccaga cgccgtggga ggagccgccg gccaagctcg
    16081 acgccaagga tgccgatgag ccgacggccg aggagctgga cgcgatcgca gccgacatga
    16141 tcggcatcga cgaccccgtc cgcatgtacc tcaaggagat cggcaaggtg ccgctgctca
    16201 acgccgagga ggaggtcgtc ctcgccaagg cgatcgagct cggcgagcag atcatcgaag
    16261 agccgcacaa ggcggtcctc tcgctgtggg agtggacacg caaccagaca gagaccaagt
    16321 cgcgcgacaa gcacccccag caccggctgc cttacaagga agagaccgag aagattgtca
    16381 cgggcgcgtt caaggcggcc cagaaggacg gcctttacca gcacacgccg gacttccact
    16441 tcgtcaaggc cggcaaggct gtcgagggcg acatcgccaa ggcgacgctc aaggaggcgt
    16501 ctcacaaggt caaggcatac aacgaggacg cgatcctcgc caacttcacg gagctggttg
    16561 acttcagctt ccgggcggcc cactcgaccg acgccgagac gcacgacaac agcgccatcc
    16621 gcgcggtcta cgagtggtca cgcggcgtgg cctgggaggc actgcggcgc tggatctaca
    16681 acggcaagga tgccgagctc atggccgaga tgggctggac gcccgagcag gacatgtcgc
    16741 tcaagccgcg cgaccggcgc ggtgtgctcg tgcgctacgg caaggaaggc cgcgagcagc
    16801 tgacgtcggc caacctgcgg ctcgtggtct ccattgccaa gaagtacatc ggccgcggca
    16861 tgtccttcct cgacctgatc caggagggca acatcggcct catccgcgcg gtcgagaagt
    16921 tcgactacga gaagggcttc aagttctcga cctacgcgac gtggtggatc cgccaggcca
    16981 tcacccgcgc catcgccgac caagcgcgga cgatccgcat cccggtccac atggtcgaga
    17041 cgatcaaccg cctgatccgc gtctcgcgcg ggctactcca ggagctcggc cgcgagccca
    17101 ccgtggagga gatcgccgag gcgatgtcgc gcggccagga agtggtcgtc acgccggaca
    17161 aggtgcgcga gatcatcaag gtgtcgcagg agccggtgag cctcgagacg ccgatcggcg
    17221 aggaagagga ctcgcacctc ggggatttca tcgaagaccg ggcagcactg gcacccgccg
    17281 aggccgcgtc ccaccagctg ctcaaggagc aggtcgaggc cgtgttggac tcgctcagcg
    17341 gccgcgagcg gcgcgtgctc cagctgcgct tcggcctcga ggacggccgc gcccgcaccc
    17401 gggaggaagt gggcaaggag ttcaacgtga cccgcgagcg catccgccag atcgaggcca
    17461 aggcgctgcg caagctgcgc cacccgtcgc gctcgcgcaa gctgaaggac tacctggagt
    17521 agcccgcccc gctcgcggcg cactaacgcc ccacgttcga ataagccgaa aaccgccgcc
    17581 aagagcctgg ttcggccttg gcttggctca aattccggtg attccgaacg ttgcgcgaca
    17641 ggattgacca gaatgcctcg ggcggccggc gcattcgaac gtgccacgta agcagccggt
    17701 aaggcctggt caattcttgg ccaagtgctt ctgtccaaca tgcagtgaat ggttgccaag
    17761 caacgcgctg tcgatcgagg aacggcgcgc ggtcgcacct tggtgtcgac gatcggcggc
    17821 gagatccggc ttgccaggca gcgtgccggc ctgagcctcg aagccgtcgc ccgcgccgca
    17881 ggcgtctcgg gtgtggaggt ttcacgcatc gagcgagcac gagctgagtg ggtttcgctc
    17941 ctcaccctgg cacggctgtg cacgatcgtc ggactcgacc tgaccgcgcg tgcctatccc
    18001 ggtgcgaacc cgctccgcga tgcacgtcac aaccgactgc tgatgttgct ccacggacac
    18061 atcaacccag cactgggctg ggcgctggag gtgccgctgc ccaatccggg cgaccagcgc
    18121 gcctgggacg cgatgatccg aggcgacggc tggcgctacg gcgtcgagtg cgagatgaac
    18181 ccgatcgacg gtcaggccgc gttgcgccgc ctcaccctca agcagaagga cggcctggtg
    18241 gatggcgtca ccctgctgct gcccgatacg cgtcaggcac ggttattccg ccgagagttc
    18301 gtcgagcagt tggctgcgca attcccggtt cgatcgatcg atgcgttgcg cgccctcgcg
    18361 gccgggaaga atcccggcgg gtcggcgatc atcacgccat gaagttcaag acgacgctca
    18421 agcaggctga gggcagcaca gcgaccggga tcgagatacc cgacgaggtt ctcgctgccc
    18481 ttggtgccgg caagaagccg ccggtcaagt tgtcggtcaa cggctaccgc tatcgcagca
    18541 cggtcgcgac cgtcagcggc gcctacatgg tcggcttcag cgccgaccat cgggccgcga
    18601 gcggcctcaa aggtggtgat ccgatcgagg tccagatcga gctcgacacc gaaccacgaa
    18661 cggtcgagct accggccgac ttccaggctg ccctcgacgc cgacacccaa gcgagggcca
    18721 cgttcgaaaa gctgtcgaac agcctcaagg gctaccacgt gtcccaggtg acgggcacca
    18781 agaaccccga gacacgccgg cgcaggatcg agaaatcgat cgcgacactc cgggccggga
    18841 agccgcgcta gcccggaacg gctgacggat cggtccggcg caggagcatt cccaggtaaa
    18901 ccttccacgg cccgacgtcg gcgtcgtgcg agccggcgag tccggcaaag atctggccga
    18961 tgtggtccag gtcgtgcacg gcccacgtgt tcaagagatt gtccagcgtc acctcaccga
    19021 gcgatgggtg cagcccaagt ttcccaaggt cgtcatcact gaccagcgcg gcgaactcgg
    19081 ccaggttctg cgcgcgcagc gcggcgaatc gatcgagcag ctcatccagc gacgcgccgc
    19141 ggtcgcgctc gagcatcgcg acccgatcga agcgatcgaa ctctcggctc gtcccgtctt
    19201 cgaggatgcg cttcgtgcgc gccagccaat cgctcagctc gccggtgatg agatgaccca
    19261 cgacgtctcg cggccgccag ccatcagggg tgtccggggt atcggtccac gtgtcgggca
    19321 atccggcgag cagtgcccgc agcacgtcgg gcgtgcgacg caggatctcc atgggctctg
    19381 cgacctgccg tgaggcctgg accgagacgg gcatcagatg ttctccgcat aagagcgttt
    19441 gatccagccg accacctcgt catcaatgtc gtcggcgctg gcgaggccga tcttgtgcgt
    19501 gaccgagctc tgcgggaagt tcttcgccgc ctccagcgtg cccgtggcag gctgcgttgc
    19561 gggtagtcgg aggcccaggt cagagcgcgt cttcgtggct ggcaccaggg cggcaaacgt
    19621 ccgcttgggg gtcaggaatg cgacatacgt cttgcgcgcc tgtacctcta tctcgcccac
    19681 gtccggcagt aacgccagca gcgcgtcgta gatgggtcgc gtggccggcc ggccggcgta
    19741 ttggttgtcg atcaggtcct cggcgctcgt ctgcaggtat tcgggatagc cgaagcgctc
    19801 catcaccagc agcatcgctg ggtagcccgt gacacctttc tccgacaacc acgcccggag
    19861 gccggcctcg tcagtcttgc cgctctcgcg cacgcgcgcg ttccacgtct cgagaccatc
    19921 gccggtgcgc tcgatcagcc acttgatctc ctgctcgcgc atctcctgcc acgaacgcgg
    19981 ccggcgctgg ctcacgagtt caggtcccac gccttcttgc gcgggagcgc ggcccactcg
    20041 tcgcgcagga tcgacatcat ctcgacgtcg aggtatcggc cctgcttgaa gacaccatgg
    20101 cgctcgacgc cttcgagtac gaagcccgac ttgtcgtacg agcgtcgggc acgcttattg
    20161 aagacgtaca cctccagcca catccgctcc aggcggagca tgccgaagcc gaagtccacc
    20221 agcgcattca tcgcgtcggt gccgtagccc ttgccccaca gcgtcttctc gccgatcgat
    20281 atgccgatgc ccgcgttgcc gttgatgtag tcgaccgcga acaggctcag gctgccgatc
    20341 ggctgatcgt tcttgagcag gcagatgacg tagtggtagt ggtccttgcc ctggcgctcg
    20401 accatgccct cgaaccactt ttcctccatg gccacgctca tcggcgcgcg catgttcagg
    20461 aatgacgtcg tctcggcgtc gttgagccag cgcacgaagc tgggcacgtc cgcgcgttcg
    20521 gccgggcgga gatagacgtg ctcgccgcgc aggatgggta ccggcctggc gccctggtcg
    20581 ttcacttgcc ctccctggaa ggctcggtcg ccatgcccga gcgcgaccag tcgctgtacg
    20641 aaccgacgta gaggattgga tcgggcagtc cggccaggcg catcgccagc gcattgtggc
    20701 aggcgttggt gccgctgccg cagtaattca ccgttggtcg gtcatcggct acaaggccag
    20761 cgaagcgcgc gataagggcg ggctgatcga ggaaataccc ggcgccgtcg acgttgtcca
    20821 tggtcggggc cgagatggcc gtcggaatgt gacccggaat ggggtcgatc ggctcgacct
    20881 cgccgcggta gcgttcgcgc gcccgcgcgt cgagcagtgt gacgctgccg agacgcacgg
    20941 ccagctcctc gcgcgcgatc acgttggtcc agtgatcggc cagctcgagg cgcgccctgt
    21001 cgtagtgcgg ctcgtctgtg ctgagcgcca tgccctgcgc gacccacgcc ttgatcccgc
    21061 cgtcgagcac cgccacgccg ccgcgatggc ccaggttgtc gagcatccac cacagccgcg
    21121 ccgcgatcgc gccgccgaca tcgtcgtacg ccacgaccaa cgaatcgctg ccgatgccca
    21181 gttcctccat ccgctctcga aacgcgtcgg cggtggggag cggatggcgg cccggcgcgc
    21241 catagccgtc gaggtcgctc aggtcatcgt cgagatcgac gaagatggcc ttgaggatat
    21301 ggccggcgtc atacgccgcg cggccggccc cgggcttgtt
//
LOCUS       DATBRX010000182         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2192968, whole genome shotgun sequence.
ACCESSION   DATBRX010000182 DATBRX010000000
VERSION     DATBRX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192968"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="mazG"
                     /locus_tag="VIF08_07125"
     CDS             <1..1141
                     /gene="mazG"
                     /locus_tag="VIF08_07125"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY8135800.1"
                     /translation="ELLDQVEARFGVTAADGLQLLSTSALVARPFDPGMALVIVPGLA
                     DAAPPIPGRAAHGASPAVLLQALYPADHLLHPLPDGVPASVGTVTDEALSAGTWYVPA
                     LEPLANVASPYALPWLVARLRAPDGCPWDREQDHMSLRKFLLEETYEVYDALEGGSTP
                     QLAEELGDLLLQIVLHAHYGAEDGVFDMADVHRAVVAKIVRRHPHVFGDAVASSASEV
                     LRNWEQIKAGERAERSAAGAESVARDQNMPEAFAGLARSLPALAYAQEMQERAALLGY
                     DWPSLEGVLDKVKEEAAEIIIARDDRERREEFGDLLLVLVNMARKLGIDSEAALRAAS
                     AKFAMRFAGVERIAAERGVDLRQLTLDELDELWQQAKRDSKVGAA"
     gene            1138..1863
                     /gene="rph"
                     /locus_tag="VIF08_07130"
     CDS             1138..1863
                     /gene="rph"
                     /locus_tag="VIF08_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY8135801.1"
                     /translation="MSIGGGQFRADGRSPTQMRKVKFMLDAQKWAEGSCIIEMGDTRV
                     LCAASVEDRVPPHLRGKGEGWVTAEYQMLPRATAERTQREASKGRQGGRTMEIQRLIG
                     RALRGVVDTTKVGDRTITIDCDVLQADGGTRTASITGGYVALVLALRKIGAEGALAGQ
                     VAAVSVGIVDALPLLDLDYSEDSHAEVDFNVVGTSGGAYVEVQGTAEGKPFDRAQMDR
                     LLELADSGLQQLFKMQRTVLGLE"
     gene            2050..2667
                     /gene="rdgB"
                     /locus_tag="VIF08_07135"
     CDS             2050..2667
                     /gene="rdgB"
                     /locus_tag="VIF08_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY8135802.1"
                     /translation="MPERRRLLLATGSQHKFAELSALLDLPNTDLVSLADLEIPDDVD
                     ETGSTFEDNARLKARHYAAASGLPTLADDSGIEVDALGGRPGVKTRRYAGADATDERN
                     NRKLLGELSGFFGPDERTARYQCVLVLVEDGALTETTRGVFEGRVAFAPRGKGGFGYD
                     PIFEPLTEKPGGRTVGQLSAEDKNRVSHRALAARAMREKLIARGY"
     gene            complement(2683..>3655)
                     /locus_tag="VIF08_07140"
     CDS             complement(2683..>3655)
                     /locus_tag="VIF08_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8135803.1"
                     /translation="LMQISTAVILILAANTAFADFPRLSSFLARDGFLPRIFQFRGER
                     LAFNSGILVLAGVAVVLMVAFGGSVTALIPLYTVGVFIAFTLSQAGMVRRWHKLRHEQ
                     SGWRKRAIINGIGAATTGVVAIEVAATKLVHGAWIVLVLIPALIAVMLFIKRQYASTA
                     AQLAVRKDIHLPGPHRQERVIVPVPGINRAVVHALNVGRSIADDVLAVLVTDSPDEGA
                     AVREQWEAQLPEVPLVVVESPYRAIVGPILAYLDVLDQMVHDEREAPITFVVIPEFVA
                     RSWWERILYNQSANRLRRALLGRPHTVVVNVPYRREQPNGNGHAAAD"
BASE COUNT          579 a         1213 c         1315 g          548 t
ORIGIN      
        1 tgagctgctc gaccaggttg aggcgcgctt cggcgtgact gccgcggacg ggttgcagct
       61 gctctcgacc agtgctctcg tggcgcggcc gttcgacccg ggcatggcgc tggtcattgt
      121 tcctggcttg gccgatgctg cgccgcctat cccgggtcgc gcggcgcacg gcgcatcgcc
      181 agcggtgctc ttgcaggcgc tctatccggc cgatcacctg cttcaccccc tgccggacgg
      241 ggttccagcc agcgtcggca cggtcaccga cgaggcgctg agcgcaggga cgtggtacgt
      301 gccggcgctc gagccgctcg ccaacgtcgc gagcccctac gcgctgccgt ggctggtcgc
      361 gcggctgcgc gcgcccgacg gctgtccgtg ggaccgcgag caggaccaca tgagcctgcg
      421 caagttcctg ctcgaggaga cgtacgaggt ctacgacgcg ctcgagggcg gatcgacgcc
      481 gcagctcgcc gaagagctag gtgacctcct attgcagatc gtgctgcacg cgcactacgg
      541 cgcggaggat ggcgtcttcg acatggctga cgtgcatcgc gcggtcgtgg ccaagatcgt
      601 gcgccgccat ccgcacgtgt tcggcgatgc cgtcgccagc agcgcgagcg aggtgctgcg
      661 caactgggag cagatcaaag ccggcgagcg cgcggagcga tcggcagcgg gcgctgaatc
      721 ggtggcccgt gaccagaaca tgccggaggc tttcgccgga ttggcgcggt ccttgccggc
      781 gctggcgtac gcgcaggaga tgcaggagcg cgcggcgctg ctcggctacg actggccgtc
      841 gctcgagggc gtgctcgaca aggtcaagga agaggccgcg gagatcatca tcgcgcgcga
      901 cgaccgcgag cggcgcgagg agtttggcga cctgctgctc gtcctggtca acatggcgcg
      961 caagctgggc atcgattccg aggccgccct gcgggctgcc agtgccaagt tcgccatgcg
     1021 cttcgccggt gtcgagcgca tcgccgccga acgcggcgtc gacctcaggc agttgacgct
     1081 cgacgagctc gatgagctgt ggcagcaggc caagcgcgat agcaaggtgg gtgcggcgtg
     1141 agcatcgggg gaggtcagtt ccgcgcggat ggtcggtcgc ccacgcagat gcgcaaggtg
     1201 aagttcatgc tcgacgccca gaagtgggcc gagggctcgt gcatcatcga gatgggcgac
     1261 acgcgcgtgc tgtgcgcggc atcggtcgag gatcgcgtcc cgccgcacct gcgcggcaag
     1321 ggcgagggct gggtcacggc cgagtaccag atgctgccgc gtgcgaccgc ggagcgcacg
     1381 cagcgcgaag ccagcaaagg tcgtcagggc ggccggacga tggagatcca gcgcctgata
     1441 ggccgggctc tgcgcggcgt ggtcgacacc acgaaggtcg gcgaccgcac gatcaccatc
     1501 gactgcgacg tcctccaggc ggacggcggc acgcgcaccg cctccatcac cggcggctac
     1561 gtcgcgctgg tgctcgcgct gcgcaagatc ggcgcggagg gcgcgctggc cggccaggtc
     1621 gccgcggtca gcgtgggcat cgtcgatgcc ctgccgctgc tcgatctcga ctactcggag
     1681 gactcgcacg cggaggtcga cttcaacgtc gtcggcacga gcggtggcgc ttacgtggag
     1741 gtccagggga cggcagaggg caagccgttc gaccgcgcgc agatggaccg cctgctcgag
     1801 ctcgccgaca gcggactgca gcagctgttc aagatgcagc gcacggtgct cggcctggag
     1861 tgagtgtcgc ggtcaccggg ctcgcttccc ggcatttgtc ctcgctggtc atctttgagc
     1921 cacccgccgg ccgcgcgggg ctcctgaccg gcggtccgcc acgctcgcac agagcacttt
     1981 ctcgccgcgc cggcccctcg aatcgggccg cgacgcgctt acggttgaca gagcagcaca
     2041 tctgccggga tgcctgagcg ccgccggctg ctcctggcga cggggtcgca gcacaagttc
     2101 gccgagctct ccgctctgct tgatctgccc aacaccgacc tcgtgtcgct ggccgatctc
     2161 gaaatccccg acgatgtcga cgagaccggg tcgaccttcg aggacaacgc ccgactgaag
     2221 gcccggcact acgctgccgc ctcggggctg cccacgctag ccgacgactc gggcatagaa
     2281 gtcgacgcgc tcggcggccg gccgggcgtc aagacgcggc gctatgccgg cgcggacgcg
     2341 accgacgaaa ggaacaatcg caagctgctc ggcgagttga gcggcttctt cgggcctgac
     2401 gagcgaacgg cccgctacca gtgcgtgctc gtgctggttg aggacggcgc cctcacggag
     2461 acaacgcgcg gcgttttcga gggccgtgtg gcattcgcgc cccgcggcaa gggcggcttt
     2521 ggctacgacc caatcttcga gccgctcacg gaaaagccgg gcggccgcac cgtgggccag
     2581 ctaagcgccg aagacaagaa ccgcgtgtcc caccgcgccc tcgcggcgcg cgcgatgcgc
     2641 gagaagctga tcgcccgcgg ctactgagtc ggcggatact gatcagtcgg cggcggcgtg
     2701 gccgttgccg ttcggctgct cgcgccggta gggcacgttg acgaccacgg tgtgtggccg
     2761 gcccagcagg gcgcggcgca accggttggc cgactggttg tagaggatcc gctcccacca
     2821 cgagcgcgcc acgaattcgg ggatcaccac gaatgtgatg ggcgcctcgc gctcgtcgtg
     2881 caccatctgg tcgagcacgt cgagataggc caggatcggc ccgacgatgg cgcggtaggg
     2941 cgactcgacc acgaccagtg gcacttccgg cagctgcgct tcccactgct ccctgactgc
     3001 ggctccttcg tcgggtgaat cggtaaccag cacagcgagt acgtcatcgg cgattgagcg
     3061 gccgacgttc agcgcgtgta ccacggcgcg gttgatgccg ggcacaggca cgatcaccct
     3121 ttcctggcgg tgcgggccgg gcaggtggat gtccttgcgc accgcgagct gtgccgctgt
     3181 ggacgcgtac tggcgcttga tgaacagcat cacggcgatg agcgcgggga tgaggaccag
     3241 cacgatccag gcgccgtgga ccagcttggt cgcggctacc tcgatggcga caacgccagt
     3301 cgtcgccgcg ccgatgccgt tgatgatcgc ccgcttccgc cagcccgact gttcgtggcg
     3361 cagcttgtgc cagcgccgga ccatgcccgc ctggctcagg gtgaaggcga tgaagacacc
     3421 caccgtgtag agcgggatca gcgccgtgac gctgccgcca aaggcgacca tgaggacgac
     3481 ggccacgccg gccagcacga ggatgccgct gttgaaggcc agccgctcgc cgcggaactg
     3541 gaagatgcgc ggcaggaaac cgtcgcgggc gaggaacgat gacagccgcg ggaagtcggc
     3601 aaacgccgtg ttggcggcca ggatcaggat gactgccgtc gagatctgca tcagg
//
LOCUS       DATBRX010000183         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_853047, whole genome shotgun sequence.
ACCESSION   DATBRX010000183 DATBRX010000000
VERSION     DATBRX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853047"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /gene="treS"
                     /locus_tag="VIF08_07145"
     CDS             complement(<1..1314)
                     /gene="treS"
                     /locus_tag="VIF08_07145"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY8135804.1"
                     /translation="MSGDPTWYKDAVIYELHVRSFADSTGDGIGDFPGLTSRLDYLAD
                     LGVTALWLLPFYPSPGRDDGYDIADYSGVNPDYGTLRDVKTFIRAAHERGLKVITELV
                     CNHTSDQHPWFQRARRAPAGSAARDYYVWSDTPDRYPDVRIIFKDFERSNWTFDDEAN
                     AYYWHRFYHHQPDLNFDNPKVRNEIKKALDFWLDLGVDGLRLDAVPYLYERDGTNGEN
                     LPETHAFLRELRAHVDANYQDRMLLAEANQWPEDAVAYFGQGDECHMCFHFPVMPRLY
                     MSLRQEDRFPLYDILDQTPAIPDNAQWALFLRNHDELTLEMVTDEERDYMYRAYAADP
                     QMRINLGIRRRLAPLLGNNRRRIELLNGLLFALPGTPVLYYGDEIGMGDNVYLGDRNG
                     VRTPMQWSADRNAGFSDANRQRLFLPVITDPEYSYEAINVDAQQAN"
     gene            complement(1311..3152)
                     /locus_tag="VIF08_07150"
     CDS             complement(1311..3152)
                     /locus_tag="VIF08_07150"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY8135805.1"
                     /translation="MPDDGRRRVVIENVAPQIDGGRFPVKRIVGDTLVVEADVFADGH
                     DEVSALVYFQPPDDGQGRPGLIAKPMEALGNDRWRSTLYLETPGRYEFYVEGTVDHYT
                     TWQHDLEKKIAAGQDVTVDRLIGEQILARSGEADVLASRTRSDTYAVTVDPPRAGFSA
                     WYELFPRSASPDPKRHGTFKDVVARLDYVSGLGFNVLYLPPIHPIGRQFRKGPNNTAS
                     SNPADPGVAWAIGGPEGGHTAVNPDLGTLDDFDALVATARAKDIDIALDIAFQASPDH
                     PWVTEHPEWFRKRPDGTIQYAENPPKKYQDIYPFDFESSDWRGLWQALNDVFRFWIGH
                     GVTTFRVDNPHTKAFAFWEWCIADIKRDHPEAIFLAEAFTRPRPMHRLAKLGFSQSYT
                     YFTWRNTAAELRTYMTELTALPAADYFRPNFWPNTPDILHADLQHGGRAAFMARVVLA
                     ATLSSNYGIYGPPYELMEAVPREAGSEEYLDSEKYQQRTWDLARADSLAPLITALNAA
                     RRDHPALQSNERLVFHDINSDWLLAYSKTSADGRDVILAVVNMDYHATRAGTLVLDLE
                     ALGLAADSSFEAHDLLDGATYTWRGARNWVSLDPEVRAAHLLLLRRP"
     gene            complement(3170..>4522)
                     /locus_tag="VIF08_07155"
     CDS             complement(3170..>4522)
                     /locus_tag="VIF08_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135806.1"
                     /translation="NTLEFDPQILKRYVNFMNNPDERTAVDQFGKGDKYFGVATLMAT
                     MPGLPMFGHGQVEGFAERYGMEYRRAYLDETPDPELVARHEREIFPLLHRRWLFAEVG
                     DFALYDFVDIDGNVNEDVFAYSNMRGGERSLVVYHNKYAETRGWIKTAVVTAKTLRES
                     LALPAGETDFVLLRDSRGGLEYLRSARELAEKGLYVELHAYETQAFLEMRELSDADGG
                     WARLALELGGRGVPSIGEALRELEMAPFHVALRTDDATAAVRQAAALIGREPPAGISA
                     DGAARAVDEYLLKERPDLARGRWIYEWHLEDVVPDADLTALRLDLAARQARKEDGASP
                     HTLLKDDRFRRLIGVNEADGVTWFNKEDFERVVTALVLPRVGDLVAAAAKSGYKLDEL
                     AKLVAEPPPWASTRPPRPAPRGGRSANTRDQSMPPRPQQKATTPPKSRPARATRTKEK
                     "
BASE COUNT          695 a         1424 c         1606 g          797 t
ORIGIN      
        1 gttggcctgc tgggcgtcga cgttgattgc ctcgtagctg tactcggggt cggtgatcac
       61 cggcaggaag agacgctgcc ggttggcatc gctgaagccg gcgttgcgat cggcgctcca
      121 ctgcatgggc gtgcgcacgc cgttgcgatc gcccaggtac acgttgtcgc ccatgccgat
      181 ctcgtcgccg tagtagagga ccggcgtgcc gggcagggcg aagagcagcc cgttgagcag
      241 ctcgatccgg cggcggttgt tgccgagcaa cggcgccagg cggcgccgga tgcccaggtt
      301 gatgcgcatc tgcggatccg cggcgtaggc gcgatacatg tagtcgcgtt cctcgtcggt
      361 gaccatctcg agcgtcagct cgtcgtggtt gcgcaggaag agcgcccatt gcgcgttgtc
      421 cgggatggcc ggcgtctggt cgaggatgtc gtacagcggg aagcggtctt cctggcgcag
      481 cgacatgtag agccgcggca tgaccgggaa gtggaagcac atgtggcact cgtcgccctg
      541 gccgaaatac gcgaccgcat cctccggcca ctggttggcc tccgccagga gcatccggtc
      601 ctggtagttg gcgtcgacgt gggcgcgcag ctcgcgcagg aacgcgtgcg tctcgggcag
      661 gttctcgcca ttggtgccgt cgcgctcgta caggtacggg accgcgtcga ggcgcaggcc
      721 gtccaccccc aggtcgagcc agaagtcgag cgccttcttg atctcgttgc ggaccttggg
      781 gttgtcgaag ttcaggtcgg gctggtggtg atagaagcgg tgccagtagt aggcgttcgc
      841 ctcgtcgtcg aacgtccagt tcgagcgctc gaagtccttg aagatgatcc gcacgtcggg
      901 gtagcggtcc ggcgtgtcgc tccagacgta gtagtcgcgc gccgcgctgc cggccggggc
      961 gcggcgggcg cgctggaacc atgggtgctg gtcgctcgtg tggttgcaca cgagctcggt
     1021 gatgactttc aggccacgct cgtgggccgc gcggatgaag gtcttgacgt cgcgcagggt
     1081 gccgtagtcg gggttgacgc cggagtagtc ggcgatgtcg tagccatcgt cccggccggg
     1141 cgacggatag aacggcagca gccagagcgc ggtcacgccc aggtccgcca gatagtcgag
     1201 gcggctcgtc agcccgggga agtcaccgat gccatcgcca gtgctgtcgg cgaacgagcg
     1261 cacgtgcagc tcgtagatga ccgcgtcctt gtaccaggtg ggatcgccgc tcatgggcgg
     1321 cgcagtaaca ggaggtgcgc ggcgcggacc tccggatcga gcgagaccca gttgcgcgcg
     1381 ccgcgccagg tgtaggtcgc gccgtcgagg aggtcgtgcg cctcaaagga tgagtcagcc
     1441 gccagaccca gcgcctctag atcgagcacg agtgtgccgg cgcgggtcgc gtggtagtcc
     1501 atgttgacca ccgcgaggat gacgtcgcgc ccgtcggcgg acgtcttcga gtacgcgagc
     1561 agccagtcgc tgttgatgtc atggaagacg agtcgctcgt tggactggag cgccggatgg
     1621 tcgcgccgcg cagcgttgag cgcggtgatc agcggcgcga gcgaatcggc gcgcgccagg
     1681 tcccacgtgc gctgttgata cttctccgaa tcgaggtatt cctctgagcc cgcctcgcgc
     1741 ggcacggcct ccatcagctc gtacggcggg ccgtagatgc cgtaattgga agacagggtg
     1801 gcggcaagga cgacgcgcgc catgaacgcc gcgcggccac cgtgctgcag gtcggcatgc
     1861 aggatgtccg gcgtgttcgg ccagaagttc gggcggaagt aatcggccgc gggcagagca
     1921 gtgagctcgg tcatgtaagt gcgcagctcg gccgcggtgt tgcgccatgt gaagtaggtg
     1981 tacgactgcg agaacccgag cttggccagc cgatgcatcg gcctgggccg ggtgaatgcc
     2041 tctgcgagga agatcgcctc cgggtggtcg cgcttgatgt cggcgatgca ccactcccag
     2101 aacgcgaacg ccttggtgtg cgggttgtcg acgcggaacg tggtcacgcc gtggccgatc
     2161 cagaaccgga acacgtcatt gagcgcctgc cagagcccgc gccagtcgct gctctcgaag
     2221 tcgaatgggt agatgtcctg gtatttcttg ggcgggtttt ccgcgtattg gatggtcccg
     2281 tcgggtcgct tgcgaaacca ctccgggtgc tcggtgaccc agggatggtc gggcgaagcc
     2341 tggaacgcga tgtcgagcgc gatatcgatg tcctttgcgc gtgccgtggc gacgagcgcg
     2401 tcgaaatcgt cgagcgtgcc cagatccggg ttgacagcgg tgtgcccgcc ttcgggacca
     2461 ccgattgccc acgccacacc gggatcggcc gggttgctgg atgcggtgtt gttggggcct
     2521 ttccggaact gccgaccgat gggatggatc gggggcaggt agagcacgtt gaagcccagc
     2581 ccgctgacgt agtcgagccg cgccacgaca tctttgaagg tgccatgacg cttggggtcg
     2641 ggcgacgccg agcgtgggaa cagctcgtac caggcggaga agccggcgcg cggcggatcc
     2701 accgtgacgg cgtaggtatc gcttcgcgtt cgtgaagcca gtacgtcggc ctcgccgctg
     2761 cgcgcgagga tctgctcacc aatcagccga tcgacagtca cgtcctgtcc ggccgcgatc
     2821 ttcttctcga ggtcgtgctg ccaggtcgtg tagtggtcga cagtgccttc cacatagaac
     2881 tcgtagcggc ccggcgtctc gagatacagc gtagaacgcc agcggtcgtt accgagcgct
     2941 tccatgggtt tggctatcaa gccggggcgg ccctggccat cgtcgggcgg ctggaagtac
     3001 accaaggctg aaacctcgtc gtgcccgtcg gcaaaaacgt ccgcctcgac gaccagcgtg
     3061 tcgccgacga tgcgcttgac cgggaatcgg ccgccatcga tctgcggcgc cacgttctcg
     3121 atgacgaccc gccgccgacc gtcgtcaggc atcgcgccct gagccttcgt cacttttcct
     3181 tcgtgcgcgt ggcgcgggcg ggccgcgact tgggcggtgt tgtggccttc tgctgaggtc
     3241 gcgggggcat ggattggtcg cgcgtgttcg ccgatcggcc accgcgcggc gcgggccgcg
     3301 gcgggcgcgt ggacgcccac ggcggcggct ccgcgaccag cttggccagc tcgtcgagct
     3361 tgtagcccga cttcgcggcg gccgcgacga ggtcaccgac gcgtggcagc acaagcgccg
     3421 tcacgacgcg ctcgaagtct tccttgttga accacgtcac gccgtcggcc tcgtttacac
     3481 cgatcagccg tcggaagcga tcgtccttca gcagggtgtg cggcgaggcg ccgtcctctt
     3541 tccgcgcctg gcgcgccgca aggtcgagtc gcaacgcggt caggtccgcg tcgggcacga
     3601 catcctcgag gtgccactcg tagatccagc gaccgcgcgc gaggtcgggt cgctccttga
     3661 gcagatattc atcgaccgcg cgcgccgccc cgtcggcact gatgccggcg ggtggctcgc
     3721 gaccgatcag cgccgccgcc tggcgaacgg ccgccgtcgc gtcgtcggtc cgcagcgcga
     3781 cgtggaacgg cgccatctcc agctcgcgca gcgcctcgcc aatgcttggg actcccctgc
     3841 caccaagctc cagggcgagg cgcgcccagc cgccatcggc gtcggacagc tcacgcatct
     3901 ccaggaaggc ctgcgtctcg tacgcgtgga gctccacgta gagacccttc tcggccagct
     3961 cgcgcgcgga gcgcaggtac tcgagtccgc cgcggctgtc gcgcaggagc acgaaatcgg
     4021 tttcgccggc aggcagtgcc agcgactcgc gcagcgtctt cgcggtaacg accgcggtct
     4081 tgatccagcc acgcgtctcg gcgtacttgt tgtggtagac gacgagcgag cgctcgccgc
     4141 cacgcatgtt cgagtacgcg aagacgtcct cgttcacgtt gccatcgatg tccacgaagt
     4201 cgtacagggc gaagtcgccg acttcggcga acagccagcg ccggtgcagc agcgggaaga
     4261 tctcgcgctc gtggcgagcg accagctcgg gatcgggcgt ctcatctagg taggcgcggc
     4321 ggtactccat gccgtagcgc tcggcaaagc cctcgacctg gccgtggccg aacatcggta
     4381 acccgggcat cgtcgccatc agggtcgcga cgccgaagta cttgtcgccc ttgccgaact
     4441 gatcaacggc cgtgcgctcg tcggggttgt tcatgaagtt gacgtagcgc ttgaggatct
     4501 gcgggtcgaa ctcgagcgtg tt
//
LOCUS       DATBRX010000184         4928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_740800, whole genome shotgun sequence.
ACCESSION   DATBRX010000184 DATBRX010000000
VERSION     DATBRX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740800"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1232)
                     /locus_tag="VIF08_07160"
     CDS             complement(27..1232)
                     /locus_tag="VIF08_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8135807.1"
                     /translation="MTIDLSLSDENKLVQQSVRDFAANEIEPHVREWDAKHEVHREVF
                     ARMGELGFLGAPIPEEYGGAGMDYVSLGILCEELERADTAFRVVMSVHVGLNSLTLLQ
                     WGSDDQKQRFLVPQARGEKLATFGLTEPGVGTDAASLATTARRAGDEYVLNGSKMWIS
                     LADVADHFLVFATVDRGRSHKGITAFIVERGMPGFTTGSIEGKLGIWAGNTGELAFNE
                     VRVPVANRVGEEGEGFTIAMSAIDQGRFTVASGAVGLAQACLDASVKYAHERKTFGQE
                     IGRHQLVQQMLAKMSVGIEAGRLLCHQVAWLKNRGLRNTRETSMAKWFCTDHAVASAL
                     DAIQVHGAYGYSDEYPVARYLRNSKAAVIYEGTSQLHTLIQADYLMGYREDKPLRCPP
                     LPAPGYERG"
     gene            1367..1774
                     /locus_tag="VIF08_07165"
     CDS             1367..1774
                     /locus_tag="VIF08_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY8135808.1"
                     /translation="MAAAFVLVFATACGGGGDTPGGPTPAQTTGPTAAITPNGEAAAE
                     KCTVGPIEGAQESHITGSHDMVPSDFTVPVGGSITWFNDSAFGHTITFADGPDCQYVL
                     VNGSVSVTFDTPGVFTYVCRIYPQYMFGTVTVQ"
     gene            complement(1806..2858)
                     /locus_tag="VIF08_07170"
     CDS             complement(1806..2858)
                     /locus_tag="VIF08_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEY8135809.1"
                     /translation="MEPTDGPLTFEELQLATRNRGMPLEALRYDITPVGLHYLLIHFD
                     IPQIDADKWRLAISGFNAPLELTYDEIRALPRTTVPVTLECAGNGRARLSPRPLSNPW
                     LNEAIGTAEWSGTSLWPILERAGVPDDAVEIVFRGADPGIQGGVEHFYERSLTLAEAK
                     RPEVILAYEMNGRPLEPQHGFPLRLIVPGWYGMTSVKWLSSIRTVGVPFTGYQQTPAY
                     HFTEGADDEGEPVQRIRPRALMVPPGVPDYFSRHRFVDAGRVTIFGRAWSGSAEVTRV
                     EVGVDGVWSEATLGESVGQFAWRSWTFAWDATPGEHVLSCRATDATGAVQPIDQPWNY
                     QGMGNNSVQTVAATVR"
     gene            complement(2929..3114)
                     /locus_tag="VIF08_07175"
     CDS             complement(2929..3114)
                     /locus_tag="VIF08_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8135810.1"
                     /translation="MGVHELGPMLTASEVASLLHLHVNTVKRLGDRGELPFFRVCKRG
                     DRRFRLDDVMQFLARNR"
     gene            3312..4406
                     /locus_tag="VIF08_07180"
     CDS             3312..4406
                     /locus_tag="VIF08_07180"
                     /inference="COORDINATES: protein motif:HMM:NF042779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOPRIM nucleotidyl transferase/hydrolase
                     domain-containing protein"
                     /protein_id="HEY8135811.1"
                     /translation="MRLASIATHDVPGLPDIDLALQPVTALIGPRASGKSSLLRAVSW
                     LLSGSPASVGQAPVPTVRAELLATDGGQRRKVERAADTMPQAPLPQVVFLTAHDRLPS
                     PDEPHHAFDSDAAAAEAMVAAIAERRLSGVEGEVLLIEEPELMLTVHQQRHFYNLLHR
                     YAERNQVIYSTRSPAMLDAVDYHEIVRLDVHAGRMNVRRAPAGTLDDEQRVRLQAEFA
                     HERTEMFFASAVVLVEGQTEAIALPLIFRRLGHHPDALGISVVQVGGKGNIPLVARLL
                     AELGIPHVVVFDSDRGRPGERENRLIKRAVGKAPVFVLDPDFEGVAGIHDHDEKVLNA
                     WRLFVGIAPAKVPPVLRAIVDKTVELANGA"
     gene            complement(4403..>4928)
                     /locus_tag="VIF08_07185"
     CDS             complement(4403..>4928)
                     /locus_tag="VIF08_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8135812.1"
                     /translation="PVRKTEIVGGYSLGFSIFATIQVALILLFVLGTINLDLPDPLPD
                     INFGLGVPIAGSPVLAFVITLLLALAAVNLGIFLSTFARTEFQILQFIPLVVVPQALL
                     AGIIWPVESLPGLLQPIARLMPMTYGIDGLREVLIKGSGLAASAVQLDIAVLAGMALV
                     LALAASLTIRREVA"
BASE COUNT          784 a         1670 c         1648 g          826 t
ORIGIN      
        1 ctccgcgctg gtcggacctt gcccggtcag cctcgttcgt agcccggcgc gggcagcggc
       61 gggcagcgca agggcttgtc ctcgcgatag cccatcaggt agtcggcctg gatgagggtg
      121 tgcagctgcg aggtgccctc gtagatgacc gcggccttgg agttgcgcag gtagcgcgcc
      181 accgggtact cgtcggagta gccgtacgcc ccgtggacct ggatcgcgtc gagcgcgctg
      241 gccacggcat ggtccgtgca gaaccacttg gccatggacg tctcgcgcgt gttgcgcagc
      301 ccgcggttct tgagccacgc gacctggtgg cacagcagcc gcccggcctc gataccgacg
      361 ctcatcttcg ccagcatctg ctgcaccagc tggtgccggc caatctcctg gccgaacgtc
      421 ttgcgctcgt gcgcgtactt caccgacgcg tcgaggcacg cctgcgccag gccgacggcg
      481 cccgacgcga ccgtgaagcg gccctggtcg atggcgctca tggcgatcgt gaagccctcg
      541 ccctcctcgc caacgcggtt cgccaccggg acgcgcacct cgttgaacgc cagctcgccg
      601 gtgttacccg cccagatgcc cagcttgccc tcgatcgagc cggtcgtgaa gccgggcatg
      661 ccgcgctcca cgatgaacgc ggtgatgccc ttgtgcgacc ttccgcgatc caccgtcgcg
      721 aagacgagga agtggtcggc cacgtcggcg agcgagatcc acatcttgct gccgttgagc
      781 acatactcgt cgccagcgcg tcgggcggtg gtcgccaacg acgccgcgtc cgtgccgacg
      841 cccggctcgg tcaggccgaa cgtcgccagc ttctcgccac gcgcctgggg cacgaggaac
      901 cgctgcttct ggtcgtcact gccccactgc agcagggtca gcgagttgag acccacgtgg
      961 acgctcatca ccacgcggaa cgccgtgtcc gcgcgctcga gctcctcgca caggatcccc
     1021 agcgagacgt agtccatccc agcgccgccg tattcctcgg ggatcggcgc gcctaggaag
     1081 cccagctcac ccatgcgcgc gaagacctcg cgatggacct cgtgcttcgc gtcccactcg
     1141 cggacgtgcg gctcgatctc gtttgcggca aaatcgcgga cgctctgctg gacgagcttg
     1201 ttctcgtcgc tcaacgacaa gtcgatggtc atgcgcgtat tgtggcgatt gcggccagcc
     1261 actagcggtg gcggcgtatt ttcgctaggt tgtagcgggg tcacgcagtc tgagtcggtc
     1321 ctccgacact gagagggatg ccttgaggaa tcgacctttg gccgtcatgg ccgccgcctt
     1381 cgtccttgtg tttgccaccg cctgcggcgg cggcggagac acacccggag gaccaactcc
     1441 ggcgcagacc accggcccca cggcggcaat cacgccaaac ggtgaagctg ccgccgagaa
     1501 gtgcacggtg ggccccatcg agggcgcaca ggaatcccac atcaccggta gccacgacat
     1561 ggtgcccagc gatttcaccg tccccgttgg cggctccatc acctggttca atgactcggc
     1621 cttcggccat acgatcacgt ttgccgatgg cccggattgc cagtatgtgc tcgtcaacgg
     1681 gtcggtttcg gtgaccttcg acacgccggg cgtcttcacg tacgtgtgca ggatctatcc
     1741 gcagtacatg ttcggaacgg tcaccgtcca gtagggctct gccaggccgc gtagccggcc
     1801 gggtgctagc ggaccgtcgc tgcaaccgtc tggacgctgt tgttgcccat tccctggtag
     1861 ttccagggtt ggtcaatcgg ctgcaccgcg cccgtcgcgt cggtcgcgcg gcatgaaagg
     1921 acgtgttcgc cgggcgtggc gtcccacgcg aaggtccacg atcgccaggc gaactggccc
     1981 accgattcac ccagcgtcgc ctccgaccaa acgccatcga ccccgacttc gaccctggtg
     2041 acctccgccg agccggacca cgcccggccg aagattgtga cccggcccgc atcgacgaac
     2101 cggtggcggc tgaagtaatc gggcacgccc ggcggcacca tcagggcgcg cggtcggatg
     2161 cgttgcaccg gctcgccttc atcgtcggcg ccctccgtga aatggtacgc cggcgtctgc
     2221 tggtagcccg tgaatgggac gccgaccgtc cggattgagc tgagccactt gacgctcgtc
     2281 atgccgtacc agcccggcac tatcagcctg agcgggaagc catgctgcgg ctccaggggc
     2341 cggccgttca tctcgtacgc gaggatcacc tccgggcgct tggcctccgc cagcgtcagg
     2401 ctgcgttcgt agaagtgctc gacgccgccc tggatccccg gatcggcgcc cctgaatacg
     2461 atctccaccg cgtcgtccgg tactcccgct cgctcgagga tgggccacag gctcgtgccc
     2521 gaccactccg ccgtgccgat tgcctcgttc agccacgggt tgctgagcgg ccgcggactt
     2581 agccgcgcgc ggccattgcc ggcgcactcc agggtgaccg gcaccgtcgt gcgtggcaag
     2641 gcgcgtatct cgtcgtaggt caattcgagc ggggcgttga agccgctgat cgccaggcgc
     2701 catttgtcag catcgatctg cgggatgtcg aagtggatga gcaggtaatg cagaccgacc
     2761 ggcgtgatgt cgtagcgcag tgcttccagg ggcatgccgc ggttgcgcgt agccagctgg
     2821 agctcttcga aggtgagcgg gccgtcggtc ggttccatcg ggccaacaat agagaaccgc
     2881 cggtaccggc ggttccgcga ttcgctattg agttggaggc gacgggactt agcggttgcg
     2941 cgccaggaac tgcatcacgt cgtcaagacg gaagcggcgg tcgccccgct tgcagacgcg
     3001 gaagaacggc agctcgccgc ggtcgcccag gcgcttgacc gtattgacgt gcaggtgaag
     3061 caggctcgct acttcactgg ccgtcagcat cggccccagc tcatggactc ccatagatgc
     3121 catcgttgcc ctcgtgtgcg gtgttgggtc gaatgctccc gttcgggagg gcatgcgcca
     3181 agccgtactt tgggaaccac ctgagcggta cgaggggtgt tgcgggcggt caacgtcggt
     3241 gagtgcgagt tagggcggtt gcagggcacc gaccaatcgt tgccccggct actctgccga
     3301 cgatgacggc catgcgcctc gcgtcgatcg ccactcacga tgtgcccggc ctgcccgata
     3361 tcgatctggc actgcagccg gtgacggcat tgatcgggcc gcgcgccagc ggcaagtcga
     3421 gcctgctgcg cgcggtgtcg tggctgctgt cgggttcgcc ggcgagtgtc ggccaggcgc
     3481 cggtgcccac ggtccgcgcc gagctgctcg cgactgacgg cgggcagcgg cgcaaggtcg
     3541 agcgtgccgc cgacaccatg ccgcaggccc cactgccgca ggtcgtcttc ctcacggcgc
     3601 acgaccgtct cccctcgccc gacgagccgc accacgcgtt cgacagcgac gccgccgcgg
     3661 ctgaggcgat ggtcgcggcg atcgcggagc ggcgcctatc cggggtcgaa ggcgaggtgc
     3721 tgctcatcga ggagccggag ctgatgctga cggtccacca gcagcgccac ttctacaacc
     3781 tgctgcaccg ctacgccgag cgcaaccagg tcatctactc gacccgctca ccggccatgc
     3841 tcgacgcggt tgactaccac gagatcgtgc gcctcgacgt gcacgccggg cggatgaatg
     3901 tgcgccgcgc gccggccggc acgttggacg acgagcagcg cgtgcgcctg caggccgagt
     3961 tcgcccatga gcgcacggag atgttctttg cgtcggccgt ggtgctggtc gaaggccaga
     4021 cagaggcaat cgcgctgccg ctgatcttcc ggcgactggg ccaccacccg gatgcgctgg
     4081 gcatctccgt cgtgcaggtc ggcggcaagg gcaacatccc gctcgtggcg cggcttctcg
     4141 cagagctcgg tattccccac gtcgtcgtct tcgattcgga tcgcggccgg ccgggcgagc
     4201 gcgagaatcg gctgatcaag cgcgccgtcg gcaaggcgcc ggtgttcgtg ctcgatcctg
     4261 atttcgaggg cgtggccgga atccacgacc acgacgagaa ggtgctcaac gcctggcgcc
     4321 tgttcgtggg catcgcaccg gcgaaggtgc cgccggtcct gcgcgcgatc gtcgacaaga
     4381 ctgtcgaact ggcgaatggg gcctaggcga cttctcgtct gatcgtcagg ctggccgcga
     4441 gcgcgaggac gagcgccatg cccgcgagca cagcgatgtc caactgcacg gcgctggcag
     4501 ccagcccact gcccttgatc agcacctcgc gcagcccgtc gatgccatag gtcatcggca
     4561 tcagccgggc tatgggctgg aggagtccgg ggagcgactc gaccggccag atgatccccg
     4621 cgagcagcgc ctgggggacc acgacgagcg ggatgaactg caggatctgg aactcggtcc
     4681 gcgcaaaggt cgaaaggaat atgcccaggt tgactgccgc cagcgcgagc agcagcgtga
     4741 tcacgaacgc gaggacgggc gaacccgcga tgggcacgcc caggccgaaa ttgatgtcgg
     4801 gcaacggatc gggcagatcg aggttgatcg tgcccaggac gaacagcagg atcagcgcga
     4861 cctggatcgt ggcgaagatc gagaagccca ggctgtagcc gccgacgatc tctgtcttgc
     4921 gcaccggc
//
LOCUS       DATBRX010000185         6431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_741228, whole genome shotgun sequence.
ACCESSION   DATBRX010000185 DATBRX010000000
VERSION     DATBRX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741228"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VIF08_07190"
     CDS             <1..567
                     /locus_tag="VIF08_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135813.1"
                     /translation="AIGAIGFAYVMGMRAKSPLVLRPLIRLQRALINPRQMRSAGTPG
                     AYAAVIRHRGRTSGREYETPVGAVATDDGFAIALVYGSRTNWLRNVLASGSATIVHEG
                     HAYEVDRPEVVPMRAVADRFTAGDQRGFRWLAVNQAVLLRRVEPGLARELEGRSVTAP
                     EPAAAARGSAGGPGVGHVPDPVVGARSA"
     gene            589..1113
                     /locus_tag="VIF08_07195"
     CDS             589..1113
                     /locus_tag="VIF08_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135814.1"
                     /translation="MHKNWFSELIAKEVEGADPHEDRGLSIATVAVTVLAGSCFIAHQ
                     AASTGFFTSAFGPAEALLLYGTTLYVVAASVLIIAGRKSLSRDLDLGGLPFSAVAIAW
                     LLAVFPFDFAHLADVLPGSTRFLVQWISNDVAQVLMALALLVQLALAAYSTVLRVAVY
                     RARSGSSPRAARPA"
     gene            1181..2797
                     /locus_tag="VIF08_07200"
     CDS             1181..2797
                     /locus_tag="VIF08_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEY8135815.1"
                     /translation="MSVEVNAAPEYANSHHLDPRAKLEILFAVLLALFMFALDQTVVG
                     VALPSIISDLKGQELYAWTITIYLLTSTISGPIYGKLSDLFGRRPIFIWAVGLFLVAS
                     VGAAFAQSMPMFIFFRGLQGLGGGAVFPLAFAVLADLYTPAERGKYSGFFGAVFGLSS
                     IVGPALGGIITDNFGWPWIFIVNVPIALISLFVIFRLLPAIKRPEAARNIDYVGAALF
                     TAAIGPFLVGLSNKASADWTDPWVGGLMLVGIIFGLVFLWWESRVEEPIVVLPLFRIR
                     TFTISVLAMFLMSFGFLTAIVYLPLWFQVVHGSTATESGYQLLPLLGALIISATISGQ
                     IVARTHRYKLLIVSSMVALTVGLYLLTNLHANTDIGVLWIWMAIAGAGIGPAFAVFTL
                     IVSAAVPMHQIGAASSGLTFFQQIGGTIGLTIASTLFASRLTDEVPKQLIAAGVPAEA
                     VSGFAQGGSTLNLTRTDLRATILAQFPQAAPIIDAIVAGIREAISVALAGALWVGIGA
                     AAIAVIAVLFLKEVPLRSSWEEGPPPAAMG"
     gene            complement(2813..2888)
                     /locus_tag="VIF08_07205"
     tRNA            complement(2813..2888)
                     /locus_tag="VIF08_07205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2853..2855),aa:Arg,seq:gcg)
     gene            complement(2912..3003)
                     /locus_tag="VIF08_07210"
     tRNA            complement(2912..3003)
                     /locus_tag="VIF08_07210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2967..2969),aa:Ser,seq:gct)
     gene            3079..3957
                     /gene="aroE"
                     /locus_tag="VIF08_07215"
     CDS             3079..3957
                     /gene="aroE"
                     /locus_tag="VIF08_07215"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY8135816.1"
                     /translation="MPKRVVLIGHPVVQSLSSALHQAAFDAKGIDARYEPNDVPLIGL
                     PDAIEALRGDDFLGANIAFPFKERVAPMVDRLTEDAQATGAVDTITREGSRLVGHNTD
                     VVGFRAALDELIGRQKMPKNAVIIGAGGGARAAVHVLITAGYQRVIVFNRHLHRAEAL
                     VRHFGRSASHMELRAMTWHESVIESELSRTKVLVNASAVADPATTSPINAELLPPELL
                     VLDLIYWPRDTKLLQDARAAGATETQNGDTMLLHQTAAAFNLWTGVDIDVDVLRQQLD
                     AARDSGNPVPAAVPVE"
     gene            4073..5044
                     /locus_tag="VIF08_07220"
     CDS             4073..5044
                     /locus_tag="VIF08_07220"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEY8135817.1"
                     /translation="MVDLLVGSALFRNTFLTGGRGHVDLPRLRAVGVNVVGLTIATRW
                     PNLRGSLSGWHYRSLGMPARVTRRDMAMAEWLLHRIHGWCDQSDGGLVIVRSVLDLER
                     CLAPGGPVGILLGVQGGHVLEGDLANLARLRAEGVRMFAPAHVMDNALVGSSTGRHSG
                     GISDYGREVIAELEAQSIVVDLAHMSTAGIAQALPLLARPFVLSHTGLADISATKRNI
                     PSALVADVGAAGGLVGIMLATRLLGGSTLDAAVRALRLAIESAGAAHVALGSDMDGAL
                     RMLIDVEGLPSLTAALLESGLSETEVSGFMGANAAQVLARGLPVESA"
     gene            5162..>6431
                     /locus_tag="VIF08_07225"
     CDS             5162..>6431
                     /locus_tag="VIF08_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135818.1"
                     /translation="MGRRSPRWPTLAIVCSLLGVFAWLAAPPAFAASVGATVTAADRP
                     AAASPANSLALTATYKVRARIYWGRGAILVESTASVTNTTAVPVGRLTFNLVALVAGD
                     AKVLEVTAGGARVHAEPKNQTIVVPLPAALAPGDRTNVTIRYRAQFNALDGSRQLLLG
                     QADSVLTAYRWIPWLSRRQPFATPNFGESWVTAASPRVTVTLISQTPLIYATSGNRVT
                     KRGHSQTFVARNVRDFNFSASPDYHVRRIAWRGVTVRTYQLTGSGDVLGEYAVDALKR
                     FSEEVGPYAYPTLNVAEAPISSGMESPALAWISTSSVPEDLARLVAHEVAHQWFYAVV
                     GNNQAQQPFADEAVATFLADTLLGSDRSSLCSAGRLDRSVYKYSRRCYPEVIYVQGAL
                     YLERYRTAVGNGPFWAGIRQYFELNRFGMGG"
BASE COUNT          951 a         2161 c         2122 g         1197 t
ORIGIN      
        1 gccatcggtg ccatcgggtt cgcttacgtg atggggatgc gagctaagtc cccgctcgtg
       61 ctccgccctc taatccggct ccagcgggcg ctcatcaacc cacgacagat gcgctcggcg
      121 ggcacgcccg gagcctacgc cgccgtgatt cggcatcgcg gccggacatc aggtcgagag
      181 tacgagacgc cagtcggcgc cgtcgccacc gatgacgggt tcgccatcgc tctcgtgtac
      241 gggtcacgca caaactggct gcggaacgtg ctcgccagcg ggtcggccac tatcgtgcac
      301 gaaggacacg cttacgaggt agatcggcct gaggtcgtcc cgatgcgggc ggtcgccgat
      361 cggttcacgg ccggcgatca gcgtggcttt cgctggctcg ccgtcaacca ggcggttctg
      421 cttcgacggg tagagcccgg actggccagg gaacttgaag gtcggagcgt caccgcccca
      481 gaacccgccg cagcggcgcg cggatcggca ggcgggccag gtgtaggcca tgtgccggac
      541 ccggtcgtcg gcgcccggtc cgcctgacca tctggaggtg cggttcaagt gcacaagaac
      601 tggttttctg aactcatcgc gaaggaggtg gagggtgccg atccgcacga ggatcgcggc
      661 ctctcgatcg caaccgtcgc cgttactgtc ctggccgggt cgtgcttcat cgcccaccag
      721 gcggcatcga ccgggttttt cacctctgcc tttggcccgg cagaggcgct cctgctctac
      781 ggaacaacgc tctacgtcgt ggcggcatct gtcctgatca tcgccggccg caagagcctc
      841 tcacgcgacc ttgatcttgg cggacttccc ttttccgcgg tcgcgattgc gtggctactc
      901 gcagtctttc cgttcgattt cgcccacctc gccgatgtcc tgccgggttc cacgaggttc
      961 ctggttcagt ggatctccaa cgacgtcgcc caagtgctga tggcactggc tttgctcgtg
     1021 cagctggccc tggcagcgta ctcgacggta ctgcgggtgg cggtttacag ggcgcgatcg
     1081 ggttcatcgc cgcgcgccgc taggcccgct taggggtaca gtccgccggt cagggagcgg
     1141 tgctgcgccg ttgccggcag agggatttga gggagttcga gtgtccgtcg aagttaacgc
     1201 tgcgcccgag tacgccaaca gccaccacct ggatccgcgg gcgaagctcg agatcctctt
     1261 cgctgtcctg ctcgcgctct tcatgttcgc cctggaccag accgtcgtgg gcgtcgcgct
     1321 gccgagcatc atctccgacc tgaagggcca ggagctgtat gcctggacga tcacgatcta
     1381 cctgctgacc tcgaccatca gcggtccgat ctacggcaag ctgtccgatc tcttcggccg
     1441 gcgtccgatc ttcatctggg ccgtcggcct gttcctggtg gcgtcggtcg gcgcggcctt
     1501 cgcgcagagc atgccgatgt tcatcttctt ccgcggcctg cagggcctgg gcggcggcgc
     1561 ggtcttcccg ctggcattcg ctgttctggc agacctctac acgccggctg agcgcggcaa
     1621 gtactccggg ttctttggtg ccgtgttcgg gctgtcgtcg atcgttgggc cggccctggg
     1681 cggaataatc accgacaact tcggctggcc gtggatcttc atcgtcaacg tgcccatcgc
     1741 gctcatctcg ctgttcgtca tcttccgact gctgccggcg atcaagcggc ccgaggcggc
     1801 gcgcaacatc gactatgtcg gcgccgcgct gttcacggcg gccattggtc cgttcctggt
     1861 gggcctgagc aacaaggcga gcgccgactg gacagatccg tgggtcggcg gcctgatgct
     1921 cgtcggcatc atcttcggcc tggtcttcct gtggtgggaa agccgcgtgg aggagccgat
     1981 cgtggttctg ccgctcttcc gcatccggac attcaccatc tccgtgctgg cgatgttcct
     2041 gatgtccttc ggcttcctga ctgccatcgt ctacctgccg ctgtggttcc aggtggtgca
     2101 cggctccaca gccacggaga gtggctacca gctgttgcca cttttgggcg cgctcatcat
     2161 cagcgccaca atttcgggcc agatcgtcgc ccgcacccac cgctacaagc tgcttatcgt
     2221 gagctcgatg gtggcgctca cggtgggcct gtacctgctg accaacctcc atgccaacac
     2281 cgacatcggc gtgctctgga tctggatggc gatcgccggc gccggcattg gcccggcgtt
     2341 cgcggtcttc acgctcatcg tcagcgccgc cgtgcccatg catcagatcg gagccgcctc
     2401 gagcggcctg accttcttcc agcagatcgg tggcaccatc ggcctgacca tcgcgagcac
     2461 gctctttgcg agtcggctga ccgacgaggt accgaagcag ctcattgccg ccggcgtgcc
     2521 agcggaagca gtgagcgggt tcgcccaggg agggtcgacc ctcaacctga cccgcaccga
     2581 tctccgcgcg accatcctcg cccaattccc gcaggcggcg ccgatcatcg acgcgatcgt
     2641 cgcgggcatt cgggaggcca tctcagtcgc gctcgccggc gcgctctggg taggcatcgg
     2701 cgcggccgcc atcgcggtca tcgcggtgct gttcctgaag gaagtcccgc tgcgctcgag
     2761 ctgggaggaa gggccgccgc cggccgcgat gggctgactg tccgaaaggg gttggcgcgc
     2821 ccggcgggac tcgaacccac gacctttagg tccgcaacct aacgctctat ccactgagct
     2881 acgggcgccc gatttgccgc tttggtgcgg ctggcggaga gggagggatt cgaaccctcg
     2941 aagcaggtta ccccacttag cggtttagca aaccgccgca ctaggcctct atgcgacctc
     3001 tccctatccg gagggccgat gatagcatcg ggcttcggtc cgccctgtct gtctgggctt
     3061 tctagagggt tgtttcccgt gcctaagcgg gtcgttctga tcggtcatcc agtggtccaa
     3121 tcgctctcga gcgcgcttca tcaggcagcg ttcgacgcga agggcatcga cgcgcgctac
     3181 gagcccaatg acgtgccgct catcggcctg cccgacgcga tcgaggcgct gcgcggcgac
     3241 gatttcctgg gcgcgaacat cgccttcccc ttcaaggagc gcgtcgcgcc gatggtcgat
     3301 cggctgaccg aggacgcgca ggccaccggc gccgtcgaca cgatcacccg cgagggctcg
     3361 cggctggtgg gccacaacac ggacgtggtc ggcttccgcg cggcgctcga tgagctgatc
     3421 gggcgccaga agatgcccaa gaacgcggtg atcatcggcg cgggcggtgg cgcgcgcgca
     3481 gccgtgcatg tgctgatcac cgccggctac cagcgcgtca tcgtcttcaa ccgccacctc
     3541 caccgggcgg aggcgctggt ccgccatttc ggtcgcagcg cgtcgcacat ggagctgcgt
     3601 gccatgacct ggcacgagag cgtcatcgag tcggagctgt ccaggaccaa ggtgctcgtc
     3661 aacgcgtcgg ccgtcgccga tcccgccacg acctcgccga tcaacgccga gctgctgccg
     3721 cccgagctgc tggtgctgga cctcatctac tggccgcgcg acaccaagct gttgcaggac
     3781 gcgcgggcgg caggtgcgac cgagacgcag aacggcgaca cgatgctgct ccaccagacg
     3841 gccgcggcgt tcaacctgtg gaccggcgtg gacatcgacg tggacgttct gcgccagcag
     3901 ctcgacgctg cgcgcgactc gggcaacccc gtcccagccg cggtcccagt ggagtgagcc
     3961 gctagccctg ggcatcctgt cgggcctcgt cgatcgggcg gtcaaccgga ccgctctgcc
     4021 cggccgacta cccgcatcgt cgactgcagc ggatctgatc cggcgcgctc cagtggtcga
     4081 cctgctggtg ggcagtgcgc tctttcgcaa cacattcctg accggcgggc gcggccacgt
     4141 cgacctgccg cggctgcggg cggtgggcgt caacgtggtc ggcctgacga tcgccacccg
     4201 ctggcccaac ctgcgcggct cgctgtcggg ctggcactac cggtcgctgg gcatgcccgc
     4261 ccgcgtgact cgccgtgaca tggccatggc ggagtggctg ctgcatcgga tccacggctg
     4321 gtgcgatcag tcggacggtg ggctggtgat cgttcgctcc gtgctcgacc tcgagcgctg
     4381 cctggccccc ggcggtccag tcgggatcct gctgggagtc cagggcgggc acgtgctcga
     4441 gggcgatctc gccaatctcg cgcgtctgcg ggcggagggg gtccggatgt tcgcgcccgc
     4501 gcacgtgatg gacaacgcgc ttgttggctc ttcgaccggc cgacattcgg gcgggatctc
     4561 cgattacggg cgggaggtga tcgccgagct ggaggcgcag tcgatcgtcg tcgacctggc
     4621 gcacatgtcg acggcgggga tcgcgcaggc attgccgttg ctggcgcggc ctttcgtcct
     4681 gtcgcatacg ggcttggcag acatcagtgc aacgaagcgc aacattcctt ccgctctggt
     4741 cgcggacgtt ggcgcggccg gaggtctcgt cggtatcatg ctcgcgacgc ggctgctcgg
     4801 cggttcgacc ctggacgccg ccgtcagggc gctccgcttg gcgatcgagt cagccggcgc
     4861 ggctcacgtt gcgctcggct cggacatgga cggcgccttg cgcatgctga tcgatgtcga
     4921 gggcctgccc tcgttgacag cggcgctgct cgaatccgga ctgagtgaga ccgaggtgtc
     4981 cggtttcatg ggagcgaatg ccgctcaagt gttggctcgg ggtctgcctg tcgaatcggc
     5041 ctgaaccggc tttcctttgg cgttaagatg ccggccacaa cctcagataa ggatgcccgt
     5101 cgtgacgaac gctccagccg tgggagcggg cgctgccgga ggcggtcggt cttctgttgg
     5161 cgtgggtcgc cgtagcccgc gctggccgac cctggcgatc gtgtgctcgc ttcttggtgt
     5221 cttcgcctgg ctcgccgctc cgcctgcttt cgccgcgtca gttggtgcaa ccgtcactgc
     5281 ggctgaccgg ccggcggcag cttcacctgc caacagcctg gcgctcaccg cgacgtacaa
     5341 ggtccgcgca cgcatctact gggggcgcgg ggcaatcctc gttgagagca cagccagcgt
     5401 gacaaacacc accgccgtgc cggtcggccg gctgaccttt aacctcgtgg cgctcgtcgc
     5461 cggtgacgcc aaggtgcttg aggtgactgc gggcggagcc cgtgtccacg cggagcccaa
     5521 gaaccagacc atcgtcgttc cgctgccggc cgcgctggcg ccaggcgatc ggaccaacgt
     5581 gaccatccgg taccgggccc agttcaatgc ccttgacggt tcgcgtcagc tgctgcttgg
     5641 ccaggcggat agcgtgctga cggcctatcg ctggatccca tggctcagcc gacgacagcc
     5701 cttcgcgacg ccgaactttg gcgagagctg ggtcacggcg gcaagtccca gggtgacagt
     5761 caccctgatc agtcagacgc cgctcatcta cgcaacatca ggcaaccggg ttaccaagcg
     5821 gggccacagc caaaccttcg ttgcgcgcaa cgtgcgcgac ttcaatttca gcgcgagtcc
     5881 cgactaccac gtacgacgca tcgcctggcg cggcgtgacc gtgcgcacct accagctgac
     5941 gggatccggc gacgtgctcg gcgaatatgc cgtcgacgcg ctgaagcgtt tcagcgagga
     6001 ggtaggcccg tacgcctatc caacgctgaa tgtcgcggag gcaccgatca gcagcggcat
     6061 ggagtcaccc gcgctggcct ggatctcaac gagcagcgtg ccggaagacc tcgcgcggct
     6121 cgtggcccat gaagtcgccc accagtggtt ctacgcggtc gtcggcaaca accaggcgca
     6181 gcagccattc gccgacgagg ccgttgccac gtttcttgct gacaccctcc ttgggtcgga
     6241 ccgctcgtcg ctgtgctcag ccggacggct ggacaggtcg gtctataagt acagccggcg
     6301 ctgctacccg gaagtcatct acgtccaagg tgcgctttac ctggagcgct accgcacagc
     6361 ggtgggcaac gggccattct gggcaggcat taggcagtac ttcgagctca accgttttgg
     6421 catgggaggc a
//
LOCUS       DATBRX010000186         6206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1523680, whole genome shotgun sequence.
ACCESSION   DATBRX010000186 DATBRX010000000
VERSION     DATBRX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523680"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1475
                     /locus_tag="VIF08_07230"
     CDS             288..1475
                     /locus_tag="VIF08_07230"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEY8135819.1"
                     /translation="MACTVEEGVVLMGVTRRTHGAARRFGAGTLAALGMMLVIAGTAL
                     AASWSAPDRLSSSGWVFQADVATMSATRAVAAYVESDGTEGSEELWLRRTTDGGVSWG
                     VPLLVAAHGHSPALAAEGMNVDLVWNAPSGRVRYARSTDGGQTFGPARFLSPLGRYAW
                     RPAVARGPGGVVAVIYEDVQNGNVAVRVSTDGGVTFAPPDILTSSGGEMGVGVAIGDG
                     VIFAAYSVGSESLRVRRSLDSGATWAGAMLITNSLFEDRISISAAGAQAYIAYTVPND
                     FPNFSQVRYRRTLDGGAHWSAAMDLAPDDWTTSDPDLGLAGGVLHAAFTLCVTDIDYC
                     EANWVFYRRSMDGTTWGNPRSVPQSSLWNAYEPSVGAHRGLVTYIGEDESAIDAFART
                     RQP"
     gene            1585..2916
                     /locus_tag="VIF08_07235"
     CDS             1585..2916
                     /locus_tag="VIF08_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135820.1"
                     /translation="MTDTAGEEQDAIQRVIDSARRLGVEIDGGEARAWIESMARESQG
                     GDVMVDVDTGVFGHRASMLDLDASDLQRFQKIAPIVGFQNKPNVSTALALSGSSAQSR
                     IQAYPADADFFERIHITAPTKDDALRILSDVIREKAMETRSGPTHRLTEVKLGTWDAE
                     VTKDGKTVKKGSPISWTPAEVESGEMNVVAADGSARTVTWAEAAQNPGWCKLDWVVAD
                     PKRGQLANASNVLDPTWEDPNGEIVPLDGFLDPYFQEVYIDTDARPLFDRIVKDLSAD
                     AVDEYVKQLEHEVWKYSVKDPNWGKVARRLYNIFRLSSHYAEAAYIRELFDEPTTALY
                     QVAALIRTLDEAAEEGSPFDREVMVDQADKLIMSAISALDGPEEAEVVAQLLRFRDVL
                     SGRSQSDRSVEVNALHSETMTTVNEYFKRRLYALPGIASYLDEIAAKGDAH"
     gene            3051..5585
                     /locus_tag="VIF08_07240"
     CDS             3051..5585
                     /locus_tag="VIF08_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEY8135821.1"
                     /translation="MPSDLRRASSRHDSKFNLDYVSYQQYAAPLGVLVQGGQVTVVSR
                     DGKQLLVGGAYYPGLRSAVSPALSAGRALTIAADVRGGLIDLPDAVRQLATRRTSLRL
                     DPTTGRLFYLVATAAPANNMYQRIDAQSGELLAAWSGVDQFTAGEGVGVKGDTKDLKF
                     HGFSTIPFSLTDRPGGIGTAWKLQSPGGADFITYDAQGGNSVTNNPVMSDASSPDNIW
                     QGTNQKAGVDAQYYAALTVEFYNQALGYDWLNDCIEDPAHQTQGFPNKVRSVVHFDFS
                     PGGLPYNNAFWDQELFFMVYGDGGTAARPLSAGQDVVSHELSHAVTQCTNNLEYRMES
                     GALNESFSDIMATAAEFAMEEPNSSNCRLAADQTTCADWLLGEDLARTASGAVIRSLA
                     NPGAEGQPSHYDDRSGKTCTSAVSGNDYCYVHTNSGIPNHAFYLMVNGGRNARCSGPT
                     DSQADCDVAVPTTSLDHATKIFFAGFSMLTTDGDMCDARNSTIAAASALWQPASATDI
                     AATILAWQAVGLGDERCDSSSADFNISLSDPTLELSAGDTGSTQLTLVRHDEAGQIDF
                     TVQTPGPATVTPNPPLSNDSSGSVIQVEANNVAADGVYPILVTATGSPSTQYAAASFV
                     IDTTDPTAAVTKVGFVAMSTISAGGALPLNVTWNATDAASGIASVELDHSPTGSGWTA
                     IHGPAASVVSPKQFTTTAGLHQFQVIATDAVGNDVTSAAFLRTLTEFQETQLAYTKTW
                     NTNTVGTPWGTTRYSTKRKATATLIFTGTDVVWIAQRGPKRGVANVFVDGVKTHVDLY
                     SSSLTERRVVFIASGLAPGQHTLTIKVKGTYKRPRVDVDGVLVLAP"
     gene            complement(5592..5801)
                     /locus_tag="VIF08_07245"
     CDS             complement(5592..5801)
                     /locus_tag="VIF08_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135822.1"
                     /translation="MEIDNERPVHSPEIPQSPEAIPDNMESPDNPALPIDPEANESED
                     EVEESSDESFPASDPPSYTGATTKD"
     gene            5897..5971
                     /locus_tag="VIF08_07250"
     tRNA            5897..5971
                     /locus_tag="VIF08_07250"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5929..5931,aa:Lys,seq:ctt)
     gene            complement(5973..>6206)
                     /locus_tag="VIF08_07255"
     CDS             complement(5973..>6206)
                     /locus_tag="VIF08_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135823.1"
                     /translation="ATEAAGATAEAAHMALAAAKLAEESASRTAAAAKEAAAIAAQDL
                     SDATSESVQADADEVRAKADYRMAEDRARDSAK"
BASE COUNT         1069 a         2077 c         2036 g         1024 t
ORIGIN      
        1 gagacgcacc tgccgttcgg cggcatgaag cagacgggca acggccaccg cgaggctggc
       61 catgcggccc tggacacgtt cagcgaatgg aagtcgatct acgtcgacta ctcagggcgg
      121 ctccagcgcg cccagatcga caaccagtag acgcgtccgg cgcgacagcg ccgccggccg
      181 tactggcctt gatgacccgt tcgtgtcttg acgcggcatc aatactggcc gcacggggtg
      241 ggcactgccc gtcgatcggg gcgagagcca gcggcgcgta gcaagccgtg gcctgcaccg
      301 tggaggaagg ggtggtcctc atgggcgtca ctcgtcgaac gcacggtgcc gcgcgccggt
      361 tcggagcagg cacgctcgcg gcgcttggga tgatgctcgt catcgccggc actgcactcg
      421 cggcgtcatg gagtgcgccc gacaggctgt cgtcgagcgg atgggtgttc caggccgacg
      481 tggccaccat gagcgccacc cgtgcagtgg ccgcctacgt cgaatcggac gggacggagg
      541 gctcggagga gctgtggctg cgccgcacca cggacggcgg cgtgtcctgg ggcgtgccgc
      601 tgctcgtggc agctcacggt cactcgcccg cgctggccgc ggagggcatg aacgtcgacc
      661 tggtctggaa cgcgcccagt ggccgcgtcc gctatgcgcg cagcaccgac ggtggccaga
      721 cgtttggccc ggcacgcttt ctgtctccgc tgggacggta cgcgtggcgg ccggcggtgg
      781 cgcgcgggcc cggtggcgtc gtggcggtga tctacgagga cgtccagaac ggcaacgtgg
      841 cggtgcgtgt ctcgaccgac ggcggcgtga cgttcgcgcc ccccgacatc ctgaccagct
      901 cgggtggcga gatgggcgtc ggcgtggcga tcggcgacgg tgtcatcttt gccgcgtact
      961 cggttggctc ggaaagcctg cgcgtcaggc gctcgctgga cagcggcgcg acgtgggccg
     1021 gcgcgatgct gatcaccaac agcctgttcg aggatcgcat cagcatcagc gcggccggtg
     1081 cacaggcgta catcgcctac accgtcccga acgacttccc gaacttcagc caggttcgct
     1141 atcggcgcac cctcgacggg ggcgcgcatt ggagcgccgc gatggatctg gcgcccgacg
     1201 actggacgac gtccgatccc gacctcggac tcgcgggcgg cgtgctccat gccgcattca
     1261 cgctctgtgt gacggatatt gattactgcg aagcgaattg ggtgttctac cgtagatcga
     1321 tggacggcac cacgtggggt aatccgcggt ccgtgccgca gagctcgctc tggaacgcgt
     1381 acgagcccag cgtgggcgcg catcgtggcc tggtcaccta tatcggcgag gacgagtcag
     1441 cgatcgatgc gtttgcgcgg acgcgccagc cctagcgtga gctgcgacgc acgagctggc
     1501 ggcagttcgt ctcatctagc gctcaccgcg ctggtattcg gcggattgcc agatatcgca
     1561 cactagtcgg agcaggaggg gcacttgacg gacaccgctg gcgaagaaca agacgcgatc
     1621 cagcgcgtga tcgactccgc ccgccgcttg ggcgtcgaga tcgacggcgg cgaggcgcgt
     1681 gcctggatcg agtcaatggc cagggagagc cagggcggtg acgtcatggt cgacgtcgac
     1741 accggcgtct ttggccaccg cgcgtcgatg ctcgacctcg acgcgtctga tctccagcgc
     1801 ttccagaaaa tcgcgcccat tgtcggcttc cagaacaagc ccaatgtctc gacggcactt
     1861 gccctatcgg gcagcagcgc gcagagccgc atccaggcct acccggcgga tgccgatttc
     1921 ttcgaacgca tccacatcac ggcgccgacc aaggacgacg cgctgcgcat ccttagcgac
     1981 gttatccgcg agaaggccat ggagacccgg agcggaccaa cccaccgcct aacggaggtc
     2041 aagctgggca cctgggacgc cgaggtgacc aaggacggca agaccgtcaa gaagggcagc
     2101 cccatctcgt ggacgccggc ggaggtcgag tcgggcgaga tgaatgtcgt agcggctgac
     2161 ggttcagccc gcaccgtgac atgggcggag gccgcgcaga acccgggctg gtgcaagctc
     2221 gactgggttg tcgccgatcc caagcgcggc cagctggcca atgcgagcaa cgtgctcgat
     2281 cccacgtggg aggacccgaa tggcgagatc gtgccgctcg acggcttcct ggacccgtac
     2341 ttccaggagg tctacatcga caccgacgcg cggccgctct tcgaccgcat cgtcaaggac
     2401 ctgagcgccg acgcggtcga cgagtacgtc aagcagctcg agcacgaggt ctggaagtac
     2461 agcgtcaagg atcccaactg gggcaaggtg gcgcgccggc tctacaacat cttccggctg
     2521 tcgagccact acgcggaggc ggcctacatc cgcgagctct ttgacgagcc gaccaccgcg
     2581 ctctaccagg tcgctgcatt gatccgcacc ctcgacgagg cggcggagga gggctcgccg
     2641 ttcgaccgcg aggtcatggt cgaccaggcc gacaagctga tcatgtcggc catctctgcg
     2701 ctcgatgggc ccgaggaggc cgaggtggtc gcgcaactgc tgcgcttccg cgacgtgctc
     2761 tccggccgct cgcagagcga ccgcagcgtc gaggtcaacg cgctgcattc ggagaccatg
     2821 accaccgtta acgagtactt caaacgccgg ctctacgcgc tgccgggtat cgcctcgtac
     2881 ctggacgaga tcgcggcgaa gggcgacgcc cactgaacaa caaatcgcgg cccatcctcg
     2941 cggcgctgat cgccctctcg gcgatggccg cgccggcaac aactgtcgcg gcgggtagcc
     3001 tgccaagcgg ccacttcacg tggaccggca gcgcggcgga gtcttttgac ttgcccagcg
     3061 acctgcgccg cgcgtcctcg cgccacgact cgaagttcaa cctggactac gtcagctacc
     3121 agcaatacgc cgcaccgctc ggcgtcctgg tccagggcgg acaggtgacc gtcgtgagcc
     3181 gcgacgggaa gcagctgctg gtcggcggcg cctactaccc gggcctgcgc tctgcggtca
     3241 gccccgcgct ttccgcgggg cgggcgctga ccatcgctgc tgatgtgcga ggtggtctca
     3301 tcgacctacc cgatgctgtc cgtcagctcg cgacgcgccg gaccagcctg cgtcttgatc
     3361 caacgacggg ccggctcttc tacctcgtcg ccaccgcggc gccggcgaac aacatgtatc
     3421 agcggatcga cgcgcaaagc ggcgagctgc tcgcggcgtg gagcggtgtc gaccagttca
     3481 ccgccggcga gggcgtcggc gtcaagggcg acacgaagga cctcaagttc cacgggttca
     3541 gcacgatccc gttcagcctg accgatcggc cgggcggcat tggcacggcc tggaagctgc
     3601 agagtccggg cggcgcggac ttcatcacct acgacgctca gggcggcaac tcggtcacta
     3661 acaacccagt gatgagcgac gcctcgagcc cggacaacat ctggcagggc acgaaccaga
     3721 aagcgggcgt tgatgcccag tactacgcgg cgttgacggt ggagttctac aaccaggcgc
     3781 tgggatatga ctggctaaac gactgcatcg aggacccggc acaccagacc cagggtttcc
     3841 ccaacaaggt ccgcagcgtc gtccactttg atttctcgcc cggtggcctg ccgtacaaca
     3901 acgccttctg ggaccaggag ctcttcttca tggtctacgg cgacggcggc accgcagctc
     3961 ggcccctgtc agccggccag gacgtcgtgt cgcatgagct gagccatgcc gtcacgcagt
     4021 gcaccaacaa cctcgagtac aggatggaat ccggtgcgct gaacgaatcg ttctcggaca
     4081 tcatggccac cgccgccgag ttcgcgatgg aagagccaaa ctcgtcgaac tgtcgacttg
     4141 cggccgatca gaccacttgc gccgactggc tgctcggcga agatctggcc aggaccgcgt
     4201 ccggcgcggt cattcgcagt ctcgccaacc ccggggcgga gggtcagccc agtcactacg
     4261 acgaccgctc gggtaagacc tgcaccagcg cggtgagcgg caacgactac tgctacgtcc
     4321 acaccaacag tggcataccc aatcacgcgt tctacctgat ggtcaacggg ggccgaaatg
     4381 cccgctgctc cgggccgact gacagccagg ccgactgcga tgtggcggtg ccaacgacca
     4441 gcctcgacca tgccacgaag atcttctttg cgggcttcag catgctcacg accgatggcg
     4501 acatgtgcga cgcgcgcaac tcgacgatcg ctgcggcctc ggcgctctgg cagccggcct
     4561 cggcgaccga catcgccgcg acgatcctgg cctggcaggc ggttggcctc ggtgacgagc
     4621 ggtgcgactc ctcatcggcc gacttcaaca tcagcctgtc ggatccgact ctcgagctgt
     4681 ctgctgggga tacgggctca acccagctga ccctggttcg ccatgacgag gccgggcaga
     4741 tcgacttcac cgtccagacc ccaggaccgg cgacggtcac gcccaacccg ccgttgagca
     4801 atgactcctc gggcagtgtg atccaggtcg aggcgaacaa cgtcgctgct gacggcgtgt
     4861 acccgatcct ggtcacggcc accggaagtc cgtctactca gtacgcggcg gcgtcattcg
     4921 tgatcgacac gacagatccg accgcagcgg taaccaaggt cggcttcgtc gcgatgtcga
     4981 cgatcagcgc cggcggcgcg ctgccgctga acgtgacctg gaacgcgacc gacgccgcga
     5041 gcggcattgc ctccgtcgag cttgatcaca gtcccacggg ctcgggctgg acagcgatcc
     5101 acggccccgc ggcgtcggtg gtcagcccga agcagttcac gactaccgcg gggttgcatc
     5161 aattccaggt catcgccact gacgcagtcg ggaacgacgt cacgtcggcg gccttccttc
     5221 gcactctgac cgagttccag gaaacgcagc tggcgtacac caagacctgg aacacgaaca
     5281 ccgtgggaac gccctggggt acgaccaggt acagcaccaa gcgcaaggcg accgcgaccc
     5341 tgatattcac ggggaccgac gtcgtctgga tcgcgcaacg cggtcccaag cgcggtgtcg
     5401 cgaacgtctt cgtcgacggc gtcaagacgc acgtcgacct ctactcgagc tcccttacgg
     5461 agcgccgcgt cgtgttcatc gcgagtggcc tggcgcccgg ccagcacacg ctcacgatca
     5521 aggtcaaggg aacttacaag cgaccgcgcg tcgacgttga cggcgtcttg gtgctcgcgc
     5581 cctagacagg ctcagtcctt ggtcgtcgcg cccgtgtagc tcggcgggtc actcgccggg
     5641 aatgactcgt cggatgactc ttcgacctcg tcctcgctct cgttggcctc gggatcgatc
     5701 ggcagtgccg gattgtcggg cgactccatg ttgtctggga tcgcttccgg tgactgcggg
     5761 atctcagggc tgtgtacggg gcgctcgttg tctatttcca tgcccagatt ctcaggccgc
     5821 cttgagtcgg tagtcattcg ccgggcagcg cgcaacggtt gcaatcgcgc gtcaggggtt
     5881 agaatccggc gctcgtgggc cgctagctca atggtagagc agctgactct taatcagctg
     5941 gttgaaggtt cgagtccttc gcggctcacc aattacttcg cagaatccct ggcccgatct
     6001 tcggccatcc tgtagtccgc ctttgcccgg acctcatcgg cgtccgcctg gaccgattcg
     6061 gacgtggcgt cactaaggtc ctgggccgca attgcggccg cctctttcgc ggctgctgcc
     6121 gtcctgctgg ccgactcctc cgccagcttg gccgcggcta atgccatgtg cgcagcctcc
     6181 gcggtcgcgc cggcagcctc tgtggc
//
LOCUS       DATBRX010000187         4958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_294936, whole genome shotgun sequence.
ACCESSION   DATBRX010000187 DATBRX010000000
VERSION     DATBRX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294936"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1038
                     /locus_tag="VIF08_07260"
     CDS             85..1038
                     /locus_tag="VIF08_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8135824.1"
                     /translation="MRPILYVSAVALILATGCKEGGTGGDPEADGAPVGPDADTGGDY
                     PPPRDDLVPSVGTAGAVDIAAWNIENFPRTADTPRVVADLITSLGLDLVAVEEIASVD
                     AFDELVERLPDHDSILSSHTYSEGSYQKVGFIYNRTLMQVDGGALLFDDQGYDFPRPP
                     LQIHVTVDDGSHPAVEFMAIVVHLKAGVGDDDRARRTAAIITLEEHLRDLVAAGDDQV
                     IVLGDFNEAIDTDEGRAVFAPFLDAPSDYTVRTLGPAEDGGVTYLPFGGRFIDHIVTT
                     AGLSAEMSPSDPIVPPLDQQLPIYGDISDHLPVVAAMPVLE"
     gene            1070..1426
                     /locus_tag="VIF08_07265"
     CDS             1070..1426
                     /locus_tag="VIF08_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135825.1"
                     /translation="MTTGPPTVTLYRPVGQPELDLIAASGYRRFPPRLPEQPIFYPVC
                     NEEYAVEIAERWNARDDGVGHVTRFAVLASYLDQFERQIVGARHHEEYWIPADELDAF
                     NDAIVGSIELVQTFHR"
     gene            2193..3374
                     /locus_tag="VIF08_07270"
     CDS             2193..3374
                     /locus_tag="VIF08_07270"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY8135826.1"
                     /translation="MRALDILHSRLTEAMDFMHSARWRALWLVARAVITGEQLWLTAL
                     GRSRPGSAHVKHSIKAVDRLLGNRQLFADRRLVYRGLASLIIPPGSCPIVLVDVVEIR
                     PRWFALVASVPFAGRSFVVYACVSRCHKPRKPALVRFLRDLGQILPSSRPVLVTDAGF
                     ESPWFDAVASVGWDFVGRVRNATKLLIDGKWVGNKQVHRLATNRARSLGCVAFPRNSP
                     TERRMVLARKPKSGHRHRYTRGGRIGKRRVDHEQRRNAREPWLLATSLTSRPSIIVAI
                     FARRMRIEQTFRDVKNFRWGWSLRHCGSRSRQRLELLLLLGAIALLVQQVIGRAAENL
                     NLAREHQANTERRRRVLSIFVLGGLVLRGADRHRVPQASISAALGQLYKSTAQFDPDD
                     G"
     gene            complement(4161..>4958)
                     /locus_tag="VIF08_07275"
     CDS             complement(4161..>4958)
                     /locus_tag="VIF08_07275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8135827.1"
                     /translation="TDGAGASAFWADRLAAAIEGARGAGFRIDRVWLRARRGAGRAFT
                     GDPPPASVVVHEDGVRFEVDVRHGQKTGLFLDQRENRRYLATLSRGRRVLNLFGYTGG
                     FSLHAERGGATRVVTVDQARPAIDAAARNIAASGLDPNKHELVCADAFAWLAEAAAAG
                     RRFDLVVCDPPSFAPRAEALDAARAAYRRLNALALAVVEPGGLLLSASCSSHMTATDL
                     REAVAAAALDTGRRVVVRSARGAASDHPVLPAFPEGDYLKLLDLHVA"
BASE COUNT          791 a         1782 c         1588 g          797 t
ORIGIN      
        1 atccaacgct gggcgaatgc aagacattcc ctcttgtcgg aattctcttc gcgatggccg
       61 gcccgatcgc tattctccgc cgccgtgcgc cccattttgt atgtgagcgc cgtcgcgctg
      121 atcctggcaa ccggctgcaa ggaaggcggg accggcggcg acccggaggc cgacggcgca
      181 ccggtcggac cagatgccga caccggcggc gactacccgc caccgcgcga cgacctggtg
      241 ccctcggtcg gcaccgccgg cgcggtggac atcgccgcgt ggaacatcga gaacttcccg
      301 cgcactgccg acaccccgcg ggtcgtcgcc gacctgatca cctcgctcgg cctcgacctc
      361 gtcgcggtcg aggagatcgc cagcgtggac gccttcgacg agctggtcga gcggctgccc
      421 gaccacgatt ccatcctgtc gagccacacc tactccgagg gcagctacca gaaggtcggc
      481 ttcatctaca accgcacgct catgcaggtc gacggcggcg ccctcctgtt cgacgaccag
      541 ggctacgact tcccgcgccc gccgctgcaa atccacgtca ccgtcgacga cggcagccac
      601 ccggcggtcg agttcatggc gatcgtcgtc cacctcaagg ccggcgttgg cgacgatgat
      661 cgcgcgcgcc gcaccgccgc gatcatcacc ctcgaggagc acctgcgcga tctggtcgcc
      721 gccggcgatg accaggtgat cgtgctcggt gacttcaacg aggcaatcga caccgacgaa
      781 ggccgcgccg tcttcgcccc cttcctcgac gccccctccg actacaccgt ccgcaccctc
      841 ggccccgccg aggacggcgg ggtcacctac ctccccttcg gcggccgctt catcgatcac
      901 atcgtcacga ccgccggcct ctccgccgag atgtcgccca gcgaccccat cgtcccgccg
      961 ctcgaccagc agctgccgat ctacggcgac atcagcgatc acctcccggt ggtcgccgcg
     1021 atgcccgtcc tcgagtagcc agcgtagcca gcgccgcgct gcggtcgcga tgacgactgg
     1081 cccgcccacg gtgacactct atcggccggt cggccagcca gagctggacc tcatcgccgc
     1141 gagcggctac cggcgatttc cgccgcgact gccggagcag ccgatcttct atccggtctg
     1201 caacgaggag tacgccgtgg agatcgccga gcgctggaac gcgcgcgacg acggagtggg
     1261 gcacgtgact cgcttcgccg tccttgcgag ctatctcgac cagttcgaac ggcagatcgt
     1321 gggcgcccgg catcacgagg agtactggat tccggccgac gagctcgatg ccttcaacga
     1381 tgcgatcgtc ggctcgatcg agctcgtgca gaccttccac cgctgacccc tgctctgccg
     1441 tgaccccgac gcttgccgat ccggcatcgc ctggccctgc ctcctgccag cgctctctat
     1501 gtctactgat cctctgccat tcatcacctc ctcgtcacac ccatcccagg ttctgcgcgc
     1561 aacctaccag gtgggtctga cagtccgtcg tcggcattga taaagggcag atcttgtcgc
     1621 gcaatccgga cgcgcgatcg atcgcgattt cctgcgctcc gagtgctgcg cgtgccggcg
     1681 gcgctctcga cggtatgcgg gacgccgcgg ctcggggcgc tcgacgtttt ttcactcgcg
     1741 cgccccctgc gcgcaacgcc tgtgccagct ctggctcggt ggaacacagc agcgatgtct
     1801 ggctgacgtc cgctggcaca gccgttgcgc gcgacgggcg cgtgctcgtg atgaaaaaac
     1861 gtcgagcacc cctcgcgggc cgcgggtccc ccttgggggg gaccacacat ggaccatcgc
     1921 gtaccgaccg ctccctccaa cacgctccag cgcgcgttcc accaactggt tccggaacca
     1981 cctcaccaac tggccccgga accacctcgc aacacgcccc ggcgcgcgtt cccgactggc
     2041 cccggaacca cctcgcaaca cgctcggtcg cgcgttcccg actggccccg gaactgatga
     2101 ttccccacga tttggaggag ccgccagaaa attgaaatgc gcgcagttct ttgctctttc
     2161 ggtagtcgcc aaaccaactc gaggagcaaa gagtgcgcgc gctcgacatt ctacacagtc
     2221 gtctgactga ggcgatggac ttcatgcatt ccgcgcgctg gcgcgcgctg tggctcgttg
     2281 cccgggcggt catcacgggc gagcagctct ggttgactgc attggggagg tcgcggcctg
     2341 gtagcgctca tgtgaagcac tcgatcaagg cggtcgatcg tctcctcgga aatcggcagt
     2401 tgttcgccga tcgccgtctt gtgtaccgag gcctggcttc actcatcatc ccgccggggt
     2461 catgtccgat tgttctcgtc gacgtcgtgg agattcgtcc gaggtggttc gcgttggttg
     2521 cgagcgttcc gttcgcgggc cgctcgttcg tcgtctacgc ctgcgtctcc cggtgtcaca
     2581 agcccaggaa gccggcgctc gttcgcttcc tgcgtgacct cgggcagatc ctgccgagct
     2641 ctcggccggt gctggtgaca gatgccggct tcgaatcgcc ttggtttgac gcggtggcga
     2701 gcgtcgggtg ggatttcgtc ggccgagtac gaaatgccac caagttactg atcgacggca
     2761 agtgggtcgg caacaagcag gtgcatcgac ttgcgaccaa tcgcgcccga agcctgggct
     2821 gcgtcgcatt tccgcggaac agtccgacgg aacgtcgaat ggtgttggcg aggaagccca
     2881 agtcaggtca ccgccaccga tacacacgag gcggtcgcat cggcaagagg cgcgtcgatc
     2941 acgaacagcg acgaaacgcg cgggagccgt ggctgctggc cacgtccttg acgtcgcggc
     3001 cctcgatcat cgtggcgatc ttcgctcggc ggatgcgcat cgagcagacc tttcgcgatg
     3061 tgaagaactt tcgctggggc tggtccctgc ggcactgcgg aagcagatcg cgacagcgac
     3121 ttgaactgct cctcctcctc ggcgctatcg ccttgctcgt gcagcaagtc atcggccgag
     3181 ctgcggagaa cctcaacctc gcacgtgagc accaggcgaa caccgagcgc aggcgccgag
     3241 tcctttcgat cttcgtgctc ggcggcctcg tgcttcgcgg tgccgatcgc caccgtgtgc
     3301 cgcaagcctc gatctccgcc gcgctggggc aactctacaa gtccactgcc caattcgacc
     3361 cggacgatgg ctgatggtcg tggggatcca tcagccccgg aaccacctcg caacacgctc
     3421 ggtcgcgcgt tcccaactgg ccccggaacc acctcgcaac acgctcctgc gcgcgttccc
     3481 aactggcccc ggaaccacct ccacgctccg gcgcgcgttc ccaactggcc ccggaaccac
     3541 ctcgcaacac gctccggcgc gcgttcccaa ctggccccgg aaccacctcc gccaactggt
     3601 tccggaacca cctccacctc gatcaagagt ccgttcatga gcagtgcctt ccttccagcc
     3661 acgtgcgccc cctctgccct ctccccaagg tgcccctcgg cggagcgccg ccgccgctgg
     3721 tctcaccgaa cgcgcgcgcg agcgcaggcg gcgtgccacc gtcatcccag cccgactcgg
     3781 tgcttcgacc gagactcggt aggtggcaag ccgcttgcgc ggagcgtgcg gagggggacc
     3841 agcggcggcg gcgcgacggg gcgcggcctt ctcagccgaa gagcagcatc gacacaggcg
     3901 cggccttctc agcctgcgag acaagagcga gtgacgcctc cactcccgta gcaaactctg
     3961 cccctatcaa gactccatcc accgtcgctg tcagacccgc gtgccaggtt gcgcgcagct
     4021 catgggagag gtgttactgg gaggtgacgc atggcagaaa ccgaatagac atagctcgcc
     4081 aggactcggt ccacggcgct cgccctggca aacgcgcgcg ggacgactga ctggtggaca
     4141 actgactgcc gacggactgc ctacgcgacg tgcagatcga ggagcttgag gtaatcgccc
     4201 tcggggaagg cgggcaggac cgggtgatcg ctggccgcgc cgcgcgcgga gcgcacgacc
     4261 acccggcggc cggtgtcgag ggcggcggcg gcaactgcct cgcgcagatc ggtggccgtc
     4321 atgtggctgg agcatgaggc gctgaggagc aagccgccgg gctcgaccac ggccagggcg
     4381 agagcgttca gccggcgata ggcggcgcgc gccgcgtcga gcgcctcggc gcgcggcgca
     4441 aagctcggcg gatcgcagac caccaggtcg aagcggcggc cagcggcggc cgcctctgcc
     4501 agccaggcga aggcgtcggc acacacgagc tcgtgcttgt tggggtcgag gccggaggcg
     4561 gcgatgttgc gcgcggccgc gtcgatggcg gggcgcgcct ggtcgacggt caccacgcgc
     4621 gtggcgccgc cgcgctcggc gtggagcgag aagccgccgg tgtagccgaa caggttcagc
     4681 acgcggcggc ctcgcgagag cgtggccaga tagcggcggt tctcgcgctg gtcgaggaac
     4741 aggccggtct tctggccgtg gcggacgtct acctcgaacc ggacgccgtc ctcgtggacc
     4801 acgaccgacg ccggcggcgg gtcgccggtg aaggcgcggc cggcgccgcg acgggcgcgc
     4861 agccatacgc ggtcgatgcg gaagccggcg ccgcgtgccc cctcgatcgc cgcggcgagc
     4921 cgatcggccc agaaggcgga ggcgcccgcg ccgtccgt
//
LOCUS       DATBRX010000188         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1970647, whole genome shotgun sequence.
ACCESSION   DATBRX010000188 DATBRX010000000
VERSION     DATBRX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970647"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VIF08_07280"
     CDS             <1..999
                     /locus_tag="VIF08_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8135828.1"
                     /translation="AGRTHYTSNYGTIELRRALSAHLERLYGVSYDPAGEICVTVGAS
                     EAVAATMAALVDPGDEVVLHEPSYVAYLPSIMFNGGTPVMVSTTAASGFALEPEQVEA
                     AITPRTKVIFLGYPCNPTGAVLGEATLRAIADIAVKHDLLVVSDEIYDRLVYGGHRHI
                     PIASLPGMRERTVLMGGFSKAYAMTGWRVGYVCAPKDLLEGIVKVHQYEIMSAPTTAQ
                     DAAVVALATAEVDVQRMTAEYDRRRRMFVDGLNAMGLPTVEPLGAFYAFPSIRPTGLT
                     SEQFSERLLFEHKVAVIPGSAFGPSGEGHVRATLATSYEDLQEALTRIRAFVSTLG"
     gene            1002..2456
                     /gene="gatB"
                     /locus_tag="VIF08_07285"
     CDS             1002..2456
                     /gene="gatB"
                     /locus_tag="VIF08_07285"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY8135829.1"
                     /translation="MAVATQTKYEVVVGVEIHVQLKTASKMFCSCSSEVRGAAPNSNT
                     CPVCLGLPGALPVINRRAVEHVLATGIAVGATTPEVTRWDRKNYFYPDLPKGYQISQY
                     DLPLCSGGTLSVETSGGLAEVGIRRAHLEEDTARLLHQDKPGGGRLSLVDYNRSGIPL
                     MEIVTEPCITSAEAARRYAEELRLLLVTIGASDAQMEEGQMRVEANVSLRLPGADKLG
                     TRVEVKNMNSFRSVERAIAFEIERQSRALDAGETLIQETRGWDDTRGATYVMRLKEES
                     EDYRYFPEPDLPPLRTDAAMIASIRAALPELPAARRARYSADYGLTANDASVIVASAG
                     DYFAAAMGTDPAPDPRIAAGLLSKIGLREQKTQPALLTQRPGTELAEVVRLRTSGAIS
                     SQSAEDVYLEHLQSGESVASIVAARGLRQISDASAVTEIIDKVIAANPAAVADFQAGK
                     EQALKFLTGQVMKESRGQANAAVVAQLLGERLRQ"
     gene            2453..2737
                     /locus_tag="VIF08_07290"
     CDS             2453..2737
                     /locus_tag="VIF08_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135830.1"
                     /translation="MSEISLGLWFVGAVLIGLAAFNIRLPLARRREMNYLADNAKRYE
                     SWRGGSRTAAGTNQVSSSDVLRDILRGQIYRWLGVGAVGVVLLIGGFFVR"
     gene            complement(2734..3219)
                     /locus_tag="VIF08_07295"
     CDS             complement(2734..3219)
                     /locus_tag="VIF08_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY8135831.1"
                     /translation="MQFNRKVAWDLLDAQEGTGPVPLLDGDEPARLLGRTRRIAMVGA
                     SPDPGRPSHGVMRQLLDAGYDVVPVNPTVESVLGQTSYATLEDAVAATGPFDMVDVFR
                     RPEHTPDIARSAVATGAKSIWLQLGVVNWEAAQIARDGGLDVVMDRCTAIEDRRRRFL
                     G"
     gene            3517..>4227
                     /locus_tag="VIF08_07300"
     CDS             3517..>4227
                     /locus_tag="VIF08_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8135832.1"
                     /translation="MVSDTAGFDDKSFNQNSLAGLQQAVTDLGITETHLQSATQEDYA
                     RNLDSLANAGCDMIVSVGFLLGDDTYTAAQAHPEIRYAIVDYAYGTEAQPNLPNLEGL
                     IYATNEGAMLAGYASASWSTTKILSTFGGQQIGPVTDFMDGFVAGANYWNAQKSDTVT
                     VLGWDPVAKTGSFTGDFSSIDNAKGVSLGFLQEDADVIMGVGGNIGQGAFEAIREQDK
                     EDSAAALGVDVDWADTHPE"
BASE COUNT          702 a         1452 c         1428 g          645 t
ORIGIN      
        1 gccggccgca cgcactacac cagcaactac ggcacgatcg agctgcgccg cgcgctgagc
       61 gcgcacctgg agcgcctgta tggcgtcagc tacgacccgg cgggcgagat ctgcgtcacc
      121 gtgggcgcgt cggaagcagt cgcagccacc atggcggcgc tcgtcgatcc cggtgacgag
      181 gtcgtgctgc acgagccgtc gtacgtggcc tatctgccgt cgatcatgtt caacggcggc
      241 acgccggtga tggtctccac gaccgccgcg agcggcttcg ccctcgagcc ggaacaggtc
      301 gaggccgcga tcacgccgcg taccaaggtc atcttcctgg gctatccctg caacccgacc
      361 ggcgccgtac tcggagaggc cacgctgcgc gccatcgctg atatcgcggt gaaacacgac
      421 ctgctcgtcg tcagcgatga gatctatgac cggctcgtct acggcggcca ccgccacatc
      481 ccgatcgcgt cgctccccgg gatgcgcgag cgaaccgtcc tgatgggcgg cttctccaag
      541 gcctacgcga tgaccggctg gcgcgtgggc tacgtgtgcg cgcccaaaga cctgctggag
      601 ggcatcgtca aggtccacca gtacgagatc atgtccgcgc ccacgaccgc ccaggacgcg
      661 gccgtcgtcg cgctggcaac ggcggaagtc gacgtacagc gcatgactgc cgagtacgac
      721 cggcggcggc gcatgttcgt cgacggcctg aacgcgatgg gcctgccgac ggtcgagccg
      781 ctgggcgcct tctacgcctt cccgagcatc aggcccacag ggcttaccag cgagcagttc
      841 agcgagcggc tgctgttcga gcacaaggtc gccgtgatcc caggttcggc cttcggcccg
      901 tcaggtgaag ggcacgtgcg cgcgaccctg gcgacgagct acgaagacct ccaggaggcg
      961 ttgacgcgga tcagggcgtt cgtctcgacg ctcggctgaa catggccgtc gcgacgcaaa
     1021 ccaaatacga ggtcgtcgtc ggggtcgaga tccacgtcca gctcaagacc gcgtccaaga
     1081 tgttctgcag ctgctcgtcg gaggtccgcg gcgcggcgcc caactccaac acctgcccgg
     1141 tctgcctggg cctgcccggt gcgctgcccg tcatcaatcg gcgcgcggtg gagcacgtcc
     1201 tggcgacggg catcgcagtg ggcgcgacca cgccggaggt cacgcgctgg gaccgcaaga
     1261 actacttcta tcccgacctg cccaagggct accagatctc gcagtacgac ctgccgctgt
     1321 gctctggcgg cacgctgagc gtcgagacgt cgggcggcct ggccgaggta ggtatccggc
     1381 gcgcccatct cgaggaggac accgcgcgcc tgctgcacca ggacaagccc ggcggcggcc
     1441 ggctgtcgct cgtcgactac aaccgctccg gtatcccgct gatggagatc gtcactgagc
     1501 cgtgcatcac ctccgcggag gctgcacggc gctatgccga ggagctgcgc cttctgctgg
     1561 tgacgattgg cgcgtcggac gcacagatgg aggagggcca gatgcgcgtc gaggccaacg
     1621 tgtcgctgcg gctgcccggt gcggacaagc tcgggacgcg cgtcgaggtc aagaacatga
     1681 actcgttccg cagcgtggag cgcgcgatcg ccttcgagat cgagcgccag tcgcgcgccc
     1741 tcgacgcggg cgagacgctc atccaggaaa cgcgcggctg ggatgacacg cgcggcgcga
     1801 cctacgtgat gcggctcaag gaggagtccg aggactaccg ctacttccct gagccggacc
     1861 tgccgccgct gcgcaccgac gcggcgatga tcgcctcaat ccgggcagcg ctgcccgagc
     1921 tgccggccgc gcggcgggcg cgctacagcg ccgactacgg gctcaccgcc aacgacgcgt
     1981 cggtgatcgt cgcgtccgcc ggcgactact tcgccgccgc catgggcacc gatccggcgc
     2041 ccgatcccag gatcgccgcg ggcctgctgt cgaagatcgg cctgcgcgag cagaagaccc
     2101 agcccgccct gctcacccag cgaccgggga ccgagctggc tgaggtcgtg cgcttgcgca
     2161 cgtcgggcgc catttccagc cagagcgcgg aggatgtcta cctggagcac ctgcagtcgg
     2221 gtgaatcggt ggcatcgatc gtcgccgcaa ggggattgcg ccagatcagc gatgcctcgg
     2281 ccgtgacgga gatcatcgac aaggtcatcg cggcgaaccc ggccgcggtc gcggacttcc
     2341 aggccggtaa ggagcaggcg ctcaagttcc tgaccgggca ggtgatgaag gagtcgcgcg
     2401 gccaggccaa cgcggccgta gtcgcgcagc tactggggga gcgcctccgc cagtgagcga
     2461 gatcagcctc ggcctgtggt tcgtgggcgc cgtgttgatc gggcttgccg ccttcaacat
     2521 ccggctgccc ctcgcgcggc ggcgcgagat gaactacctc gcggacaacg ccaaacgcta
     2581 cgagtcatgg cgcggcggct cgcgcacggc ggccggcacc aaccaggtct cgagcagcga
     2641 cgtcctgcgc gacatcctgc gcgggcagat ctatcgctgg ctgggggtcg gtgcggtcgg
     2701 ggtggtgctg ctgatcggcg gtttcttcgt ccgctagccc aggaagcgcc gccgccgatc
     2761 ctcgatggcc gtgcagcggt ccatcacgac atccagcccg ccgtcgcgcg cgatctgcgc
     2821 ggcctcccag ttgacgacgc ccagctgcag ccagatcgat ttcgcccccg tcgccaccgc
     2881 tgagcgggct atgtcgggcg tgtgctccgg gcggcggaac acgtcgacca tatcgaacgg
     2941 gccggtggcg gcaacagcgt cctccagcgt ggcgtaggag gtctggccaa gcaccgactc
     3001 gacggtcggg ttgacgggca ccacgtcgta gccggcgtcg agcagctggc gcatgacgcc
     3061 gtgcgagggc cggccggggt cgggcgaggc cccgaccatg gcaatccggc gcgtcctgcc
     3121 caggagccgc gccggctcgt cgccgtcgag cagcggtacc ggccctgtgc cctcttgagc
     3181 gtcgagcagg tcccaggcga cctttcggtt aaattgcacg caccaagttt gcgccggtca
     3241 tgtggcgttg tcatactcac agcggactct cgtcgtgagc gcccagctga attgcggcgc
     3301 gtagacggag ccagaggagg acatatcttg cgtagggcta cgtttgccat tgcggcaaca
     3361 ttcgtattcg tgcttagtgc gtgctcgccg gcggcgtcgc cgtcgcccgc accgagcgga
     3421 acaccggcgt cgagcgcgag cgccgcgccg agcgcgaccc cggcttcgag cgcaaccccc
     3481 gcgccgtcag catcggcgat gtcgtacaag gcctgcatgg tcagcgacac ggccggtttc
     3541 gacgacaaga gcttcaacca gaactccctg gccggcctgc agcaggccgt cacggatctg
     3601 ggcatcaccg agacgcacct gcagtccgcc acccaggagg actacgcgcg caacctcgac
     3661 agccttgcta acgctggctg cgacatgatc gtctccgtcg gattcctgct cggcgacgac
     3721 acctatacgg cggcgcaggc gcatccggag atccggtacg ccatcgtcga ctatgcctac
     3781 ggcacggagg cgcagcccaa cctgcccaac ctcgaaggcc tcatctatgc cacgaacgaa
     3841 ggcgccatgc ttgctggcta cgcgtcagcc tcgtggtcga cgaccaagat cctgagcacc
     3901 tttggtggcc agcagatcgg cccagtgacg gacttcatgg atgggttcgt agccggcgcc
     3961 aactactgga acgcgcagaa gagcgacacg gtcacggttc tcggctggga tccagtcgcc
     4021 aagaccggct ccttcacggg cgacttcagc agcatcgaca acgccaaggg ggtcagcctc
     4081 ggcttcctcc aggaggatgc cgatgtgatc atgggcgtcg gtggcaacat cggtcagggt
     4141 gccttcgagg cgatccgcga gcaggacaag gaggactccg ctgcagccct gggtgtggat
     4201 gtcgactggg ccgacaccca tcccgag
//
LOCUS       DATBRX010000189         8495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3201272, whole genome shotgun sequence.
ACCESSION   DATBRX010000189 DATBRX010000000
VERSION     DATBRX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3201272"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VIF08_07305"
     CDS             complement(<1..186)
                     /locus_tag="VIF08_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135833.1"
                     /translation="MLRIVLALVLFAHAVGHILGPLQLLGISNVKSAWQGDTWLLSGL
                     GPTLNQVLGVVLWSVAMA"
     gene            complement(274..1062)
                     /locus_tag="VIF08_07310"
     CDS             complement(274..1062)
                     /locus_tag="VIF08_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8135834.1"
                     /translation="MHPADATPLFVSQFGAGRPLLCLHGIEAHGLRFVGLAARLRDVH
                     VVAPDVRGHGHSPGSGPWTVARHVADLAPLLESLGPGTVVLGHSYGGLIAWELARALP
                     GAMRGLVLVDPAIGVSAATAEEGIAANAAHLSWPDERSALEAHLAQRLPGAWWAAALE
                     YAAALERTDDGRLRPSVREEAVVAGWAQMREPISATSWRGPTLLLEAALENSKYVSAM
                     TATSLRNDLGDALDHRLLNVTHTIPSDHPDLLAELVDAFLKRLG"
     gene            1138..2397
                     /locus_tag="VIF08_07315"
     CDS             1138..2397
                     /locus_tag="VIF08_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135835.1"
                     /translation="MADRRLTLVVSAALVFGACSGGPAATSTPGSGATATPATSTPPT
                     GSPTSAPTDAATPTAAPATATPLPSPGAAEIAAWASDICAINATFLSDNQQITLDIPE
                     PTERTLEALKQVNANRQPRIEQGYADAVDALHALTPYPGGESFRLGLLNEFQSALDAM
                     PAFFQAIADATTVEEIDAQYTTRGTVNSAGLLGSARSVVEMDVAFSDAVAAVTTGCRF
                     FEFDARARVHGIETPDFSEIVIDETFDEPGNWPTGDFDGGTVTIADGALTVAFDSAVV
                     QGVETVGQPTSKFHDARIESHFAMNGSALVGLSCRGSRAERYYVLVNAIGLILIYRTS
                     DNSLIGRRAAPDDFDASLGLDLAIECLAGNLDPFSLIVYLNEERVAEVQDAHALESEG
                     FAGMLAQAFTPGASATFDSFRVLVPAD"
     gene            complement(2410..3387)
                     /locus_tag="VIF08_07320"
     CDS             complement(2410..3387)
                     /locus_tag="VIF08_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135836.1"
                     /translation="MTWNSAASESRDRMLIAAVWIISLGSVFLIQQYFSWSWTQAWPL
                     WVLFVGVGSLGSAIFGRAQSAFGIWGIWWPLAILAVGAVLLLSTTGNLALAPGELIAL
                     WPVAVIAVGVWFLIGAVFARPNPTMQSQSLSIPVSGLTEAWVKLNFGGGDLVVGQAEP
                     GTLVSGTFEGGVRQTTHGVGRLELSPFADAWPLMWDRPMRWNVGLASEIPVSLELHSG
                     ANRSSVDLSALRIPRLEIHTGASETRVRLPVSGATAVRAEAGVASLTLEVPQGVAARV
                     RSRMAIGSTNVDETRFPRTMDGWASPDFEGAANRVEIDVSGGLGSIRVS"
     gene            complement(3392..3709)
                     /locus_tag="VIF08_07325"
     CDS             complement(3392..3709)
                     /locus_tag="VIF08_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8135837.1"
                     /translation="MSYTFFADLAAEAPAPLRGILSLTLSDEPGVELVMFGMAAGERL
                     SEHTAARPAIVHVLSGRGELLVDGDPHELAPGTWLRMATGTPHAIVATAPLVFALYLL
                     PKE"
     gene            complement(3799..5037)
                     /locus_tag="VIF08_07330"
     CDS             complement(3799..5037)
                     /locus_tag="VIF08_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8135838.1"
                     /translation="MTTVTHHVPGAVFIEREHLVPLDHARQDGPRITVFSREVAAPGG
                     EDKPYLLFLQGGPGSEATRPTAPPTLWMKRALADYRVLLLDQRGTGRSTPVGRQIPGA
                     SAQEQADYLKHFRADSIVRDSELIRAELIGNERWSVLGQSFGGFSSITYLSIAPQGLR
                     EVYITGGLTPIGRSADDVYAITWQRVIAKTDAYFERYPEDRARTNEIFRRLANEDVRL
                     PGGDRLTVERFRLGGLWLGMSDGWEKMHNLLELPFGSPGFLWDAEPIGTNFARNPLYA
                     TLHESSYADGVATRWSAARTRPPEISADGYFAGEHMYPEMFQDYGGLRDHAAAAEILA
                     QHQWPRLYDADVLRHNDVPVAAAVYTHDLYVDRDFSLETAATIRGTKVWETAEFEHNG
                     LRADGERVLGTLFDLLHGAA"
     gene            5070..5789
                     /locus_tag="VIF08_07335"
     CDS             5070..5789
                     /locus_tag="VIF08_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY8135839.1"
                     /translation="MEGKRIEGPGPGLRATLLTSLPFAAAIGVFGVVFGAAASAIIDP
                     LLVVGMSLIIFSGSLQFALVSLLVSGAGPAALLFTTVVLNLRHVVFGAVLRPRLEAPL
                     WRRALLGFFMIDESFGLALAAGRRAALVLAVSGAAFYVAWQVGTILGVFGARALALEE
                     VARAIFPVLFIGLAALTARGKEGMVRGLAAAGLVVLSALLVPGLFDYAPIIAAILVAL
                     VPSRGAAATRTTEGAITGGAP"
     gene            5803..6105
                     /locus_tag="VIF08_07340"
     CDS             5803..6105
                     /locus_tag="VIF08_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY8135840.1"
                     /translation="MTWELVLAIGAITFVSRASSVVLLPQLPARVRHVLDRMPAAIFA
                     GLAAHSLVVPGGGLADIHTLAAAAGAVIVAPLRSLPVCLVAGLGGYVVGDLALRLI"
     gene            6190..>8495
                     /locus_tag="VIF08_07345"
     CDS             6190..>8495
                     /locus_tag="VIF08_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY8135841.1"
                     /translation="MKLPISAPRRFRLAAPRRWKAGFSLPVAILVVGVVVGMAVLIAE
                     IAAGQLRQTAADAAVHQVEAIVRGYVDPTITEDTLALDAVANPEIGEELDRLTVSGDI
                     REISIWSRDGRIVYSSSELLRGRRFSIGNLVATAFQGVSTSAYGPADAALLPGHTKGQ
                     LNVEIYVPIRGNVDGNPIGVYMVEQDARPIYSRVETTRQQVFWVALVAASVVLVILVI
                     AFAGASALLARRNALLRRRAQAERALAQDLRRSEERFRSLVRNATDVVLIADADGLIR
                     YMSPAVSRVLGHDPAGRVGEPIYDVIHPSDVEWIRSLVAETSSREGAEATAEYRARHA
                     DGSWRWLQATIKNLLGDPAVGGIVINYRDVTERRSLETQLRHQAFHDALTGLPNRALL
                     LDRLEHALTRSRRDPASLAVLFLDLDDFKTVNDRLGHHAGDELLVTVAERLRRCLRDA
                     DTAARMGGDEFAIVLEESADRAGAVRVAERILAALREPYQVAGSTVHIQGSMGISLYD
                     GPELTADEMLRLADVAMYAAKGQGKNRLTVYQAGIHAAAVDRHQLRADLQEALDSGQL
                     SLAYQPIVDLADTRLVGTEALLRWDHPTRGPIPPTEFVPIAEESGLILPIGRWVLQEA
                     CRQAREWQSRYGSSVSVSVNVSAHQVAADGLAELVTDTLRDTGLGPGGLTLEITESVL
                     LGDVERVIARLGELKSLGVRIAIDDFGIGYSSLSYLRFLPVDVLKIDRSFVNAVDSGA
                     AERAVVRSIVSLSQVLGLRTVAEGIETT"
BASE COUNT         1294 a         2950 c         2913 g         1338 t
ORIGIN      
        1 agccattgcg acgctccaca ggacgacgcc aagcacctgg ttcagggtcg gaccgaggcc
       61 gctcagcagc caggtgtcac cctgccacgc ggacttcacg ttcgagatcc cgagcaattg
      121 cagcggccca aggatgtggc cgacggcgtg ggcgaagagc accagggcga ggacgatgcg
      181 aagcatgtgc tatctccgtt gcggttatgt ccaaggtcgc tcgcggcggc gcgagacgac
      241 agtgcgcaac ggcccattgg gcgctaggca aaactacccc aaacgcttca agaaggcgtc
      301 gaccagctcc gccaggagat cggggtggtc ggacgggatc gtgtgggtca cgttgagcaa
      361 acgatggtcg agcgcgtcac ccaggtcgtt tcgtagcgac gtcgcggtca tcgccgagac
      421 gtacttgctg ttctccaggg cggcttccag caggagtgtt gggccgcgcc atgaggttgc
      481 gctgatcggt tcgcgcatct gcgcccaccc ggccacgacc gcctcctcgc gcacgctggg
      541 gcgcagccga ccatcgtcgg tgcgctcgag cgcggccgcg tactcgaggg ccgcggccca
      601 ccaggcgccg ggcaggcgtt gcgcgaggtg cgcctccagg gcggagcgct cgtccggcca
      661 cgagaggtgc gccgcgttcg cggcgatccc ctcctctgcg gtggcggcgg acacgccgat
      721 cgccgggtcc acgagcacga gcccgcgcat ggcgcccggc aaggcgcgtg ccagctccca
      781 ggcgatcagg ccgccgtacg aatgaccgag cacgaccgtc cccggcccga gcgactccag
      841 caacggcgcc aggtcggcaa cgtgccgcgc caccgtccac gggccgctcc ccggtgaatg
      901 gccgtggccg cgcacgtcgg gcgcgaccac gtggacatct ctcagccgcg cggcaagacc
      961 gacgaagcgc agcccgtgcg cctcgatgcc gtgcaggcac agcagcggcc ggccggcgcc
     1021 gaactgcgac acgaagagcg gcgtggcgtc agcagggtgc acgatcgcga ttgtcgtcgc
     1081 tagactccca gccaggcgcc tgcaaccgcg ccggttgctc gacggagggg aactcccatg
     1141 gctgatcgcc ggctcacgct cgttgtcagc gccgcgctcg tgttcggtgc atgctccggc
     1201 gggcccgccg caacctcaac cccgggcagc ggcgcaaccg cgacgccagc aacctcgact
     1261 cctccgaccg gaagcccgac aagcgcaccc acagacgcag ccaccccaac ggctgcccca
     1321 gcgacggcga cgcccctgcc cagccctggc gcggcggaga ttgctgcctg ggcgtccgac
     1381 atctgcgcca tcaacgcgac cttcctgagc gacaaccagc agatcacgct ggacattcct
     1441 gagcccaccg agcggactct ggaggcgctc aagcaggtca acgccaaccg ccagccgcgc
     1501 atcgagcagg gatatgcgga cgcggttgat gcgctccacg ccctgactcc gtatccgggc
     1561 ggcgagtcct tccggctggg actgctgaac gagttccagt cggcactcga cgcgatgcca
     1621 gcgttcttcc aggcgatcgc ggacgcgacc acagtcgagg agatcgacgc ccagtacacg
     1681 acgcgcggca cggtcaacag cgccggcttg ctcggtagcg cccgctcggt cgtcgagatg
     1741 gatgtcgcgt tctcagacgc cgtcgcagcc gtaacgaccg gctgtcgctt cttcgaattc
     1801 gacgcgcgcg cccgcgtgca cggcatcgaa acgcccgact tcagcgagat cgtgatcgac
     1861 gaaacgttcg acgaaccggg caactggccg acgggtgact tcgacggtgg cacggtcacc
     1921 atcgccgacg gagcgctgac agttgccttt gactcggccg tagtccaagg cgttgagaca
     1981 gtcggccagc cgacgtccaa gttccacgac gcacggatcg agtcgcactt cgccatgaat
     2041 ggctccgccc tggtcgggct gtcctgtcgc ggcagtcgcg ccgaacgcta ctacgtgctc
     2101 gtcaacgcaa tcggactgat cctcatctac cgcacgagtg ataactcgct gatcggcagg
     2161 cgcgccgcgc cggacgattt cgatgcctcc ctggggctcg atctcgcgat cgagtgcctc
     2221 gccggcaacc tggacccgtt ctcactcatc gtctatctca acgaggagcg agttgccgag
     2281 gttcaggatg cgcatgcgct ggagagcgag ggtttcgcgg gcatgctcgc gcaggccttc
     2341 acgcccggcg cgagcgcgac gttcgacagc ttccgggtac tggttccggc cgactgacgg
     2401 ccgctcgcgt cacgagacgc gaatgctccc gaggccgccc gagacgtcga tctcgacgcg
     2461 gttggccgcg ccctcgaagt ccggtgatgc ccagccgtcc atggtgcgcg ggaagcgcgt
     2521 ctcgtcgaca ttggtggagc caatcgccat gcgcgagcgc acgcgcgcgg ccacgccctg
     2581 aggcacctcg agggtcaatg aggcgacgcc ggcctcggcg cgtaccgctg tcgcgccgct
     2641 caccggcagg cggacgcgcg tctcgctggc gccggtgtgg atctccaggc gcggaatgcg
     2701 cagcgccgac agatcaacgc tcgagcggtt cgcgccgctg tgcagctcca ggctgaccgg
     2761 gatctcgctg gccaggccca cgttccagcg catcggccgg tcccacatga gcggccatgc
     2821 atcagcgaac ggcgatagct ccagccggcc cacgccatgg gtggtctggc ggacgccgcc
     2881 ctcgaaggtg cccgacacga gcgtgcctgg ctcggcctgg ccgactacca gatcaccccc
     2941 gccgaagtta agcttcaccc acgcctccgt caggccgctg acgggaatgc tcaggctctg
     3001 tgactgcatg gtggggttcg gtcgggcgaa caccgcgccg atgaggaacc agacgccgac
     3061 cgcgatgacg gccacgggcc agagcgcgat gagctcgcct ggcgccagcg ccaggttgcc
     3121 cgtcgtgctg agaagcagca cggcacccac ggcgaggatc gccagcggcc accagatgcc
     3181 ccagatgcca aacgccgact gggcacgccc gaagatcgcg gagcccaagc tcccgacgcc
     3241 aacgaacagc acccacagcg gccaggcctg cgtccagctc cagctgaagt actgctggat
     3301 caggaagacc gatccaagac tgatgatcca gacggcggcg atgagcatcc gatcgcgcga
     3361 ctcagacgcg gccgagttcc atgtcatggc gttactcctt gggcaacagg tacagggcga
     3421 agaccagcgg cgcggtggcg acgatcgcgt gaggcgtgcc cgtggccatg cgcagccacg
     3481 tgcctggcgc gagctcgtgc gggtcaccgt cgacgagcag ctcgccccta cccgacagga
     3541 cgtggacgat cgccggccgc gccgccgtgt gctctgacag gcgctcccct gcggccatgc
     3601 cgaacatcac cagctccacg ccgggttcgt cggacagggt caggctcaag atgccgcgta
     3661 gtggcgcggg tgcctctgcg gccaggtccg cgaagaacgt gtaactcatg ccgcgggcgg
     3721 cgtgctccta cggtgtggcg agacgactcc tctgaatcgt gtcgccgagc cggttccgcg
     3781 gggagtgccg aacggccctt aggcggctcc gtgcagcagg tcaaacaagg ttcccaacac
     3841 gcgttcgccg tccgcgcgga ggccgttgtg ctcgaactca gcggtctccc agaccttggt
     3901 gccgcggatg gtggccgccg tctcgagcga gaaatcgcga tccacgtaca ggtcgtgggt
     3961 gtagaccgcg gcggccaccg gcacgtcgtt gtggcgcagc acgtccgcgt cgtagaggcg
     4021 tggccactgg tgctgggcca ggatctccgc ggcggcagcg tgatcgcgca ggccgccgta
     4081 gtcctggaac atctcggggt acatgtgctc gcccgcgaaa tagccgtcag cgctgatctc
     4141 tggcggccgc gttcgggcgg cgctccagcg ggtcgccacg ccatcggcgt acgacgactc
     4201 gtgcagcgtc gcatatagcg ggttgcgtgc aaagttggtg ccgatcggct cggcatccca
     4261 caggaagcct ggcgatccga agggcagctc gagcaggttg tgcatcttct cccagccgtc
     4321 gctcatgccc agccagagac cgcccaggcg gaagcgctca accgtcagcc ggtcgccgcc
     4381 tggcagtcgg acgtcttcgt ttgccagccg gcggaagatc tcgtttgtcc gcgcacggtc
     4441 ctccgggtag cgctcgaagt aggcgtcggt cttggcgatg acgcgctgcc aggtgatcgc
     4501 gtagacatcg tcggcggacc gaccgatggg cgtcaggccg ccggtgatgt acacctcgcg
     4561 caggccctgc ggcgcgatcg acaggtaggt gatggagctg aagccaccga acgactggcc
     4621 gagcacgctc cagcgctcgt tgccgatcaa ctcggcgcga atgagctccg agtcgcggac
     4681 gatggagtcg gcccgaaagt gcttcaggta gtcggcctgc tcctgcgccg atgctccagg
     4741 gatctgccgg ccgaccgggg tggagcgccc tgttccgcgc tggtcgagca gcaggacccg
     4801 atagtcggcc aaggcgcgct tcatccacag cgtcggcggc gccgtgggcc gcgtggcctc
     4861 ggaccctggc ccgccctgga ggaagagcag gtacggcttg tcctcgccgc ctggcgccgc
     4921 gacctcccgg ctaaagaccg tgatcctggg accgtcttgc cgggcgtggt cgagtggcac
     4981 aaggtgctcg cgctcaatga acacggcccc ggggacgtgg tgggtgacgg ttgtcatgtg
     5041 ttgaaggcta gccgcgtagc atcgggtccg tggagggcaa gcgaatagag ggccctgggc
     5101 ccgggctgcg cgccacgctg ctcacgtcgc tgccgttcgc ggctgccatc ggcgtgttcg
     5161 gcgtggtctt tggcgccgcg gcaagcgcca tcatcgaccc gctcctcgtc gtcgggatgt
     5221 cgctcatcat cttctccggc tcgctccagt tcgcgctggt gtcgttgctg gtgagcggcg
     5281 cggggccggc ggcgctcctc ttcacgaccg tcgtccttaa cctgcgccac gtggtgtttg
     5341 gcgcggtcct gcggccgcgg ctcgaagcgc cactgtggcg tcgcgcgcta ctgggcttct
     5401 tcatgatcga cgagtcgttc ggcctcgccc tcgccgccgg ccggcgcgcg gcgctcgtgc
     5461 ttgcggtgag cggcgccgcg ttctatgtcg cgtggcaggt cgggacgatc ctcggcgtgt
     5521 tcggcgcgcg tgccctggcg ttggaggagg tcgcgcgggc gatcttcccc gtcctgttca
     5581 tcggtctggc ggcgctcacg gcgcgcggca aagagggcat ggtgcgcggg cttgcggccg
     5641 cgggcctggt cgtgctctca gccctgctcg tgcccgggct cttcgactac gcgccgatca
     5701 tcgctgcgat cctggtcgcc ctggtgccgt cgcgcggtgc ggccgcgacc aggacgacgg
     5761 agggcgccat cacggggggc gcgccatgac ggggggcgcg ccatgacgtg ggagctcgtc
     5821 ctggcgatcg gcgcgatcac cttcgtcagc cgggcgtcat cggtcgtgct gctgccgcag
     5881 ctgccggcgc gcgtgcgcca tgtgctggat cggatgcccg cggcgatctt tgctgggttg
     5941 gcggcgcatt cactggtcgt gcccggcggc ggcctggccg acatccacac cctcgccgcc
     6001 gccgccgggg cggtcatcgt tgcgccgctg cgctcgctgc cggtgtgcct ggtggctggc
     6061 cttggcggct acgtggtggg tgacctggcg ctacgcctca tctaggcgac agtaccaacg
     6121 gctcatggcc cgcaagggcc ccgcaaccgt actgtccact cccccgccga gtgcagggga
     6181 gcgtgctgca tgaaactgcc catctccgcc ccgcgccgct tccgccttgc ggccccgcgc
     6241 cgttggaagg ccggtttctc gctgccagtg gcaatcctcg tggttggcgt cgtggtgggc
     6301 atggccgtct tgatcgccga gattgccgcc gggcaactgc gtcagacagc cgcggacgcg
     6361 gcggtccacc aggtcgaggc aatcgtgcgc ggctatgtcg atcccacgat caccgaagac
     6421 acgctcgcgc tcgacgctgt cgcgaaccct gagatcggcg aggagctcga tcggcttacc
     6481 gtgtctggag acatccgcga gatcagcatc tggtcgcgcg atggacgcat cgtctattcg
     6541 agcagcgagc tgctgcgcgg ccggcgcttc tcgatcggca atctggtcgc cacggccttc
     6601 cagggcgtga gcacgagcgc ctacggcccg gccgatgccg ccctgctgcc gggccatacc
     6661 aagggccagc tcaacgtcga gatctacgtg cccatccggg gcaacgtgga tggcaatccg
     6721 atcggcgtgt acatggtcga gcaggacgcg cgacccatct acagccgcgt ggagacgacg
     6781 cgccagcagg tgttctgggt ggcactcgtc gccgcgtcgg tcgtgctggt gatcctcgtg
     6841 atcgcattcg ccggcgcatc ggccctactg gcgcgccgta acgccctgct gcgccggcga
     6901 gcgcaagccg agcgcgccct cgcgcaggat ctgcgtcgga gcgaggagcg cttccgatcg
     6961 ctggtgcgca atgcgacaga tgtcgtgctc atcgccgacg ccgacggcct catccgctac
     7021 atgagcccgg ccgtgtcgcg cgtcctgggc cacgaccccg ctgggcgggt gggcgagccc
     7081 atctatgacg tcatccaccc atctgacgtg gagtggatcc gatcactcgt tgccgagacg
     7141 tcgagccgcg aaggagctga ggccacggcg gaataccgcg cccggcacgc cgacggttcg
     7201 tggcgctggc tccaggccac gatcaagaac ctgttgggtg atccggccgt cggcggcatc
     7261 gtcatcaact accgcgacgt aaccgagcgt cgctcactcg agacccagct gcgccaccag
     7321 gcgttccacg acgccctcac cggcctgccc aaccgcgccc tgttgctgga ccgcctcgag
     7381 cacgcgctga cccgctcccg ccgcgatccg gcctcgctgg cggtcctctt cctggatctc
     7441 gacgatttca agacggtcaa cgaccggctg ggtcaccacg ccggtgacga gctgctggta
     7501 acggtcgccg agcggctgcg gcgctgcctg cgcgacgcgg acaccgcggc gcggatgggc
     7561 ggtgacgagt tcgccatcgt gctcgaggaa tcggccgacc gtgctggggc cgtgcgcgtg
     7621 gccgagcgca tcctcgccgc attgcgtgag ccgtaccagg tcgcaggctc gaccgtccat
     7681 atccagggca gcatgggcat ctcgctgtac gacggccctg agctgaccgc tgacgagatg
     7741 ctgcgcctgg cggatgtcgc gatgtatgcg gccaagggcc agggcaagaa ccgactgacc
     7801 gtgtaccagg cgggcataca tgcggccgcg gtcgaccgcc accagctgcg cgccgatctg
     7861 caggaggcac tcgactccgg gcagctcagc ctcgcctacc agcccatcgt ggacctcgcc
     7921 gacacgcggt tggtgggcac ggaggcgctg ctgcgctggg accatccaac gcgcggcccg
     7981 attccaccga ccgagttcgt gcccatcgcc gaggaaagcg gcttgatcct gcccatcggg
     8041 cggtgggtgc tccaagaggc ctgccggcag gcgcgcgaat ggcagagcag gtacggctcg
     8101 tcggtgtcgg tgagcgtgaa tgtatcggcc caccaggtgg cagccgacgg cctcgccgag
     8161 ctggtcacgg acacactccg cgacacgggc cttgggccgg gtggcctgac gctcgagatc
     8221 acagaaagcg tgctgctcgg cgacgtggag cgcgtcatcg cccgcctggg cgagctcaag
     8281 tcgctgggtg tgcgcatcgc gatcgacgac ttcggcatcg gctactcgtc cctcagctac
     8341 ctgcgcttcc tgcccgtcga cgtcctgaag atcgaccgct ccttcgttaa cgcggtcgac
     8401 tccggggcgg ccgagcgcgc ggtcgtgcgc tcgatcgtct cgctcagcca ggtcttgggc
     8461 ctgcgcaccg tggccgaggg gatcgagacc accga
//
LOCUS       DATBRX010000190        16961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3427453, whole genome shotgun sequence.
ACCESSION   DATBRX010000190 DATBRX010000000
VERSION     DATBRX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 16961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16961
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3427453"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VIF08_07350"
     CDS             complement(<1..534)
                     /locus_tag="VIF08_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8135842.1"
                     /translation="MVSAQSRVSAVPVSPQMADVCVVGGGGHVGLPLSLALADAGLKV
                     CVYDINEQTIARIERGEMPFLETGADELLAKVLPTGRLTLASDPSALRGAPIVIVVIG
                     TPIDEFLNPSMTLFDRVIDQVAPHISDGALVVMRSTVYPGTTERVARMLAERDVKVDV
                     ACCPERIAEGHALEELKN"
     gene            648..1625
                     /locus_tag="VIF08_07355"
     CDS             648..1625
                     /locus_tag="VIF08_07355"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY8135843.1"
                     /translation="MTTAPAAPSPKPLAPAPERLPTEPEAIFVCGVARSGTTMMRYVL
                     ETSPRIAIARENHFMGHVLGRRGARHFFRDAGDLKDDAAVRRVVDMIYSGEYERRAGW
                     RRPSPFWFWLKDTVARDEVERRLLAAERSERGMFQTFLRIYADASGRPVMGEKTPTHL
                     NYVDTLLEWFPGARVIHMLRDPRAVYVSDKYRRQKRGRWPYSWFDKVPGVLSTYLLIL
                     TIIAWRRAARLHPRLAAAHPEQYRMFRFEDVVEKPTEILPQVFEFVGVAMPDSAMRVQ
                     TPPAHGMRSGEEGIDPKAASRWRERIHPFARRILELFLSGPMRRYGYVD"
     gene            1747..3612
                     /locus_tag="VIF08_07360"
     CDS             1747..3612
                     /locus_tag="VIF08_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8135844.1"
                     /translation="MSSRTWHASVLRQITVAALACVAAATWLGARPLPLAGAERPNIV
                     VFYLDDAAPHDGRLWNDPARTPAIYNTFVAHGTHFRQAFGEDPMCCPGRASLLTGLHS
                     HNNGVMTNDARLFGPQETVAGALQDAGYTTMWIGKYMNLNNKLSSAGWAAHMAPWTVF
                     DGMFGANGAFYNYKVRTKDTGDISYRGVHSTQMVADRSVMRIGAAPANKPIFAVLSVF
                     NIHAPNIPMPVDEELLEKCDDMPPWWTPAYNEADVSDKPAYIQALPLLPDPDGWPMDA
                     YCREMFGVDRLVERVTDELEAVGRLDNTLLVFTADNGMAWGAHRREFKNVPYATAVPL
                     YMAWPGGWGNAPRVVDDYVSNIDLAPTFCALGGCALGPYPSGQSAPDGLSLVDLLDNG
                     SPLGRDALLESALFQDPTRDVPPWAAVRTTPQSGLGMWHYVEYADGERELYDLASDPY
                     ELDNLAGDPGRAAIAASLAQRLGELLDEGRVNRPDLSIWSMTDNAYAGYNLFASTPSP
                     AQTLTVSVVRRRTYTFRIDLANHKVSTDSFTVTAAVTGGHVRLTWLLDGVDVTGQMSS
                     GGVRFESLASMSSGSLTLQVKIKRRFPRGRTTIDLVARPVSSPAQDDHVTLVVRR"
     gene            complement(3613..4362)
                     /gene="fabG"
                     /locus_tag="VIF08_07365"
     CDS             complement(3613..4362)
                     /gene="fabG"
                     /locus_tag="VIF08_07365"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEY8135845.1"
                     /translation="MIDLTGKAAIVTGGSRGIGKAIALRLAAQGADICFSYRGNADAA
                     AGTQKEIDALGRRCVAVQADVTEPDAAEALVKAALDAFGKVDILINNAGITRDDLIMR
                     MKPEDWRDVLETNLFGAFWSLKAVTRPMLRAKSGRIISITSVSGQAGQMGQANYSSAK
                     AGLIGLTKAAARELGSRGITVNAVAPGFVLTELTQDLNDDLKAQINERTPLGRFGTVE
                     EIAYAVAFLASDEAAYITGQVLAVDGGLVMM"
     gene            4446..5939
                     /locus_tag="VIF08_07370"
     CDS             4446..5939
                     /locus_tag="VIF08_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8135846.1"
                     /translation="MSAIRTRTVIVLTLCALLAPQVASGAPQPPPPPDVRPNIVLMLI
                     DDFPAMDERVFERLPNIKALFLDKGVNFTNYWANFSLCCPGRAAILTGQTAAHNGVIL
                     NDARLLDATETIATELQGVGYHTIIAGKYLNKTGKLSVKLPPGWDQAAIKDSPPYYSY
                     PLWVNGVRETRGRSPSDYSTRVMAGYARQFLENAPVYKPLFAYFAPNATHGGPDEDSF
                     KDYKLPVVDPAHVDDPRCSGIPNWNPASYNEPDVADKPAYIRNLGPVPYPTGWPLRKA
                     CESLLSVDDWLGAIVDELRNEGRYNNTLFVLTSDNGMGWGAHRLPGKVAPYTAQMPLY
                     VSWPAKSGPTQRIDETLLSNIDLAPTFCELARCEMGPYANGYAVDGQSFLGLIDASSS
                     ESRPERDSILIEGGGGGVPPFRAIMTTADHPLGQWFLIAYVGVRTKELYDVSGGQCVE
                     WQAGDPGDPCMLVNVASSRKSIRAALMSELNDEWSGPLPGSRRAEAP"
     gene            complement(5936..7126)
                     /locus_tag="VIF08_07375"
     CDS             complement(5936..7126)
                     /locus_tag="VIF08_07375"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY8135847.1"
                     /translation="MTPRSVRITGWGMYAPERRLTNADLERVVDTSDEWIVSRTGIRE
                     RRIAARHETTATLAAIAGKRAIAVAGLQPDDIDVILVATLTPDYWMPSTGALVKEAIG
                     NTRAAAMDVAAACSGLVYAYSVADAYLRSGMFKNALVVGSETLSRYLDFSDRGTCILF
                     GDGAGAVVLSASDEEGGGMQGMELTTDPDGAYMIWLPSGGSRSPASGQTLARGEHYVR
                     MEGRETYRYATKTMATTALKAIERAGWKPEEIDLFIPHQANVRIIESVAKGLGLPMDK
                     MFVNLDRYGNTSAASVGIALAEAVDSGRIKIGDKIVLVAFGAGLTSGGIALEWTADPM
                     NGKRAEAVEPVAQVREPLDWSSVDPIPDRLRQVLEGNAGPRVPLDDVVPGEPEPAPRD
                     EVLA"
     gene            complement(7123..7635)
                     /locus_tag="VIF08_07380"
     CDS             complement(7123..7635)
                     /locus_tag="VIF08_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8135848.1"
                     /translation="MSAAGAKRAAQAGVPQTDVAGIVGLIDRLEKLLNASELTEMEIE
                     VGETALVLRKPGAFAAAAEAGAGGHSMAAPAATVAPQPSATAPTQEPAMNAVVAPLTG
                     LFYASPSPGAEPYVKPGTQIMVGQVIGLIEAMKLFNEIKSDRAGRVVRVVPEDGALVK
                     AKDPLIEVQP"
     gene            complement(7632..8897)
                     /gene="fabF"
                     /locus_tag="VIF08_07385"
     CDS             complement(7632..8897)
                     /gene="fabF"
                     /locus_tag="VIF08_07385"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8135849.1"
                     /translation="MTNGRRRIVVTGMGAVTPLGNDVATFWSRLVAGESGVDTIRINF
                     DPERLTSKIAGEVKDFDPSHVLDRKEIRRNDRYTQLALVATAEAMKQAGLPERLEGEL
                     ADRTGVIIGSGIGGSGTLVEQVTINAMRGPDRISPFFIPMAIANLAAGQSAISFGARG
                     PNWATVSACATAGHAIGEAYETIIRDDADMMFAGGSEAAVFEALVGGFAAMRALSTRN
                     DDPQGASRPFDEGRDGFVIAEGAGVLVLEELEHARRRGATVLAELVGYGATGDANHIT
                     LPAPGGEGGARAARRAIEKAGLQPHDIDHISAHATSTPEGDPAELMGFKAVFGDHIPN
                     VSITATKGAIGHTLGAAGGIGAIAAIQAMREGRVPPTLNLQEPAPEADGLDLTPLVAR
                     ERAVRAALVNAFGFGGQNAALVFRRWDAS"
     gene            complement(8894..9646)
                     /locus_tag="VIF08_07390"
     CDS             complement(8894..9646)
                     /locus_tag="VIF08_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY8135850.1"
                     /translation="MALRLAVLGDIHGNIAAFEAALADAKKHKPDRYVITGDLTFNGP
                     RPGDVIDRVRTLEKGGALVVAGNTDLAVADGDYAAAWPWLDDVPQTHIAAAEWAKDQL
                     SDEQLDYLRRLPSERRLWDGDTLLLACHGSPGSISAGLPTDLDPTVTVERVTRTDARV
                     ICCGHTHVADVRELGRKMIVNPGSCGYAFDNDPAACWALLTLPDGEDAQPSAELFRPA
                     YDAAAAAEEVAARGLAGDVYRAATIRTGRLIR"
     gene            complement(9646..10101)
                     /gene="fabZ"
                     /locus_tag="VIF08_07395"
     CDS             complement(9646..10101)
                     /gene="fabZ"
                     /locus_tag="VIF08_07395"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEY8135851.1"
                     /translation="MSQIVLTTAQIQKLIPHRWPILMVDRIVEYDPARGYIRGEKAVT
                     ATEWFFQGHFPELPVMPGVLQVEALAQTMAVYVAQQEGFGDRIGLFAAIDECRFKRVV
                     QPGDVISLEVTMDKLGGRFGRGHGVAKVEGEVACEADLSFIIPREGVLG"
     gene            complement(10114..11484)
                     /gene="accC"
                     /locus_tag="VIF08_07400"
     CDS             complement(10114..11484)
                     /gene="accC"
                     /locus_tag="VIF08_07400"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY8135852.1"
                     /translation="MFSRILIANRGEIALRIVRACRSMGITSVVAYSEADRESRAVQL
                     ADEAICVGPAESRRSYLSAPSILSAAIVAGCEAIHPGYGFLSEDDTFAEMTRAHGLTF
                     IGPSPEVLERFASKAGTRTLLAKHGLPTIPGSGMLRDDAHGMAEADKIGYPVLIKPSA
                     GGGGKGMRMVRSPRELQQAMTICRSEALAAFGDDSLYLEKWLEDNRHVEVQVMVDRFG
                     NGVHVGERDCSVQRRHQKLLEEAPSPALNAEQRAALLDAAIKAVVAAGYENVGTLEFL
                     LDRQGNWYFIEINCRIQVEHPVTEMISGLDIVAEQIRIAAGEPLSVKQSDIELKGHAI
                     EFRINAEDVAHDFRPAAGRIETYLAPGGPGVRMDSHLYSGYDVPPYYDSLLGKLIVWG
                     PDRATAIARSRAALDELVVDGVVTNVPLHRALLANEAFLNGEFTTNLLDRVGSAAFMA
                     AADAAA"
     gene            complement(11477..11965)
                     /locus_tag="VIF08_07405"
     CDS             complement(11477..11965)
                     /locus_tag="VIF08_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8135853.1"
                     /translation="MTGSNETRTDERATIARLTDEVVPTLIERLTRSDLGEIEVRQDG
                     WRVRLRRGSGDVVAAAGHATGQTQHHKPAAVAHAADAQPRREPVRGQVSSPAVGYFTP
                     RDGVAVGGRLRKGDLIGVVDMLGVRQDVISPIDGVLKQIEVESGQAVEFGQAIGRVEG
                     NV"
     gene            complement(11969..12985)
                     /locus_tag="VIF08_07410"
     CDS             complement(11969..12985)
                     /locus_tag="VIF08_07410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEY8135854.1"
                     /translation="MAERTATQAPPVEATPDELRAAVWQRVQQARNVKRPHTLELVHA
                     IASEVIELHGDRLFRDDPAIVGGFCRIDGRRAVFVGHQKGAETDENIRRNFGMPHPEG
                     YRKAIRLFRLAERLGLPVVTFIDTPGAFPGPASEERGVAEAIARAIMVMTGLRTPIVT
                     VITGEGGSGGALALAVGDVVLALENSIYSVISPEGAASILWRSAEFSQQAAMAMRLTA
                     AEQHALGVIDEVVPEPANGAHADPDETAKRLRTRIAAHLDSLAGRDRKALLDGRYARY
                     RNMGEFAVAAETGEAQPERPGIVARIRRLLDSGRAALVGGEAAAPAEPDDTPDQSTRT
                     GEGM"
     gene            complement(12978..13904)
                     /gene="accD"
                     /locus_tag="VIF08_07415"
     CDS             complement(12978..13904)
                     /gene="accD"
                     /locus_tag="VIF08_07415"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEY8135855.1"
                     /translation="MPGFKLPFGRPKQEYPPNLWTKCPSCDEMLYNKQLDKNQHVCTK
                     CGHHFRLRLGARIAQLLDRDSFREQDANLESVDMLGFVDQKPYPERLAAARATTGMRD
                     AAAWGTATIAGTRVVVCIMDFAFMGGSMGTVVGEKVTRAAEHALEERLPLIIVSSSGG
                     ARMQEGTLSLMQLAKTVAALGRLGDAGVPFISILTDPTTGGVFASFSALGDVNIAEPK
                     ALIGFAGARVAAGTIAEELPEGFQRAEFLYEHGFVDMVVQRSKLRETVLGLLRYMKPN
                     AHVERAGLNGSSALGRSAQLLDELIRREPARG"
     gene            complement(13907..14278)
                     /locus_tag="VIF08_07420"
     CDS             complement(13907..14278)
                     /locus_tag="VIF08_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135856.1"
                     /translation="MFREAEEGAIQSDAYIDALLSGHARLPVALPVAHASPAPSVRRV
                     IRTLERGMPRFHPSFLFEERLAEQLRALAEERHAGTPVAPLVLRERRVVIGGAIASGV
                     SLAGAAMWAWRHRPRADEGQD"
     gene            complement(14278..14913)
                     /locus_tag="VIF08_07425"
     CDS             complement(14278..14913)
                     /locus_tag="VIF08_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8135857.1"
                     /translation="MLRRGKDRVAALVDLDADRTAVEAARRDPRAFEALYRKYVAQVY
                     NFALYELRDPHAAEDLTAQVFLRALGGLRHFHEQAEPPQSSFRVWLFQIARNALSNER
                     RRSRRHPEAPLDAALDLPAADNVQDAAAQRAELRAALAAIDLLPPDRRRALVLRFVNE
                     LDAREIGQVLGRSEGATRVLIHRSLQAVARSIGRSRGSEKGRAAQRRRATN"
     gene            15005..15940
                     /locus_tag="VIF08_07430"
     CDS             15005..15940
                     /locus_tag="VIF08_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEY8135858.1"
                     /translation="MRHYHGFIADSDRWQRFKMRDDDVVITTPSKSGTTWMQTIVGML
                     LRGTTDLPPISTISPWIDMQIRTEEELFRVLDAQTWRRFVKTHTPFDGLPYNPHVTYI
                     AVIRHPLDVALSDRDHGLNMKEDETVALREAVAGKFERPAQWEEPPDDPASFLRWFID
                     NSRPPTGSGPNGLDDYCQQVRTYWDVRDRPNLHLFHYQDMWNDLDGEMRRVAAALGVA
                     IDEATWPGYVEAATLKSMRGRAAKAAPDGHLDIWKSHEDFFRAGGTRDWAQLLTPAEL
                     AHFDDRLRDLAGDAYDWIMRGKAALPGKAPTAAHD"
     gene            complement(15937..16716)
                     /locus_tag="VIF08_07435"
     CDS             complement(15937..16716)
                     /locus_tag="VIF08_07435"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY8135859.1"
                     /translation="MTFPSRLEHFRAVDSTQRVVREWLEAGEPEVCVAVADHQTAGRG
                     RLGRDWIAPAGSALLLSAGFRPQVLAPRHGWRLAATAALAMRDAAEEAAGLADGTIWL
                     KWPNDLVAADERRGVVKVGGVLGESVSTARRIQSAVIGIGVNGNWKAADFPWLLGATM
                     TSLRQLSGNRPIDHAALLDAFLARLEPRYEALNAGTFDAAGWTATQITTGRQVVVEVG
                     GESILGRATGVDPESGALLLARDEKTLMSIDSGEVTRCRIT"
     gene            complement(16713..>16961)
                     /locus_tag="VIF08_07440"
     CDS             complement(16713..>16961)
                     /locus_tag="VIF08_07440"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135860.1"
                     /translation="PHRAAHFVALPGLIAAARDAGALGAALSGAGSTVIALCDGAAAA
                     RDVAAAMEAYAASAGVGGRATVVRPTATGARHLTDQAP"
BASE COUNT         2664 a         5858 c         5753 g         2686 t
ORIGIN      
        1 gttcttcagc tcctctagcg cgtggccttc cgcgatgcgc tccgggcagc acgccacgtc
       61 caccttcacg tcacgttcgg ccagcatccg tgccacccgc tcggtcgtgc ccgggtaaac
      121 cgtcgagcgc atcacgacga gcgcgccatc gctgatgtgc ggcgccacct ggtcgatgac
      181 ccggtcgaac agcgtcatcg acgggttgag gaactcgtcg atgggcgtgc cgatgacgac
      241 gatcacgatg ggcgcgccgc gcaaggctga cgggtcgctc gccaaggtca ggcgtccggt
      301 cggcaagacc ttggccaaca gctcgtccgc accggtctcg aggaagggca tctcgccgcg
      361 ctcgatgcgc gcgatcgtct gttcgttgat gtcgtagacg cataccttga gccccgcgtc
      421 agcgagcgcc agcgagagcg gcaggcccac gtgcccgccg ccgccgacga cacagacgtc
      481 ggccatctgc ggcgacaccg gcacggcgct tacccggctc tgtgcagaaa ccatgaacgg
      541 agattacctg cccagactga acgccgagcg ctgctagact tcagcctgcc ccgcgcaaga
      601 ggccgcgcat tcgagcgcgc ccgcggtggt ccatttcgtg gagtccgatg accactgcgc
      661 ctgctgcgcc gtcgccgaag ccacttgcac cggcaccgga gcgcctgccg acggaacccg
      721 aggcgatctt cgtgtgcggc gtcgcccgca gcggcaccac gatgatgcgc tacgtgctcg
      781 agacctcgcc gcgcatcgcc attgcccgcg agaaccattt catgggccat gtcctgggcc
      841 gtcgcggggc gcggcacttc tttcgcgacg cgggtgatct gaaagacgac gcagccgtgc
      901 gccgcgtcgt cgacatgatc tactcgggcg agtacgagcg ccgcgctggc tggcgcaggc
      961 cgagcccctt ctggttctgg ctcaaggaca cagtcgcgcg cgacgaagtc gagcgccggc
     1021 tgctcgccgc cgaacggagc gagcggggca tgttccagac cttcttgcgc atctacgcgg
     1081 atgcgagcgg gcggcccgtc atgggcgaga agacgccgac ccacctgaac tacgtcgaca
     1141 ccctgctcga gtggttcccc ggagctcggg tcattcacat gttgcgcgat ccgcgcgccg
     1201 tctacgtttc cgacaagtac cggcgtcaga agcgcggcag gtggccatat tcgtggttcg
     1261 acaaggtccc cggcgtgctg agcacctacc tgctgatcct gacgatcatt gcctggcggc
     1321 gcgcagcgag gctgcatccg cgcctggcgg cggcgcaccc cgagcagtac cggatgttcc
     1381 gcttcgagga cgtcgtcgag aagcccactg agatcttgcc gcaggtgttc gagtttgtcg
     1441 gggtggcgat gcctgactcc gccatgcggg tgcagacgcc gcccgcgcat gggatgcgca
     1501 gcggcgagga aggcatcgat ccgaaggcgg cctcgcgctg gcgtgagcgc atccatccgt
     1561 ttgcgcggcg catcctggag ctcttcctgt caggacccat gcgccgctac gggtacgttg
     1621 actaaccgac ccgaccgccg cgggcgacgg ccacgcgcgc ttgacaggtc cacgaacatt
     1681 gcggcggaag tgcgtccggg ctggcatatg ggccgtcggg tgcgcgctgg gaggggatgc
     1741 gcggacatgt cgtcacgaac gtggcacgcc agcgttctac ggcagatcac tgttgctgcg
     1801 ctcgcctgcg tggccgcggc gacctggctg ggcgcgcgac cactgccgtt ggccggtgcc
     1861 gagcggccta acatcgtggt gttctacctc gacgatgcgg cgccgcacga cggccgcctg
     1921 tggaacgatc cggcacgcac gccagcgatc tacaacacgt tcgtcgccca tggcacgcat
     1981 ttcaggcagg cgttcggcga ggacccgatg tgctgccccg gccgggccag cctgctgacc
     2041 ggcttgcaca gccacaacaa cggcgtgatg acgaacgacg cacgcctgtt cgggccccag
     2101 gagaccgtcg ccggtgctct gcaggatgcc ggctacacga cgatgtggat cggcaagtac
     2161 atgaacctca acaacaagct gtcgtcggcc gggtgggcgg cacacatggc gccgtggacc
     2221 gtgttcgacg ggatgttcgg cgccaacggc gccttctaca actacaaggt ccgcaccaag
     2281 gacaccggcg acatttctta caggggggtc cactcgacgc agatggtcgc cgaccggtcg
     2341 gtcatgcgca tcggtgctgc gccagcgaat aagccgatct tcgccgtcct gtcggtgttc
     2401 aacatccatg cgcccaacat cccgatgccc gtcgacgagg agctcctcga gaagtgcgac
     2461 gacatgccgc cgtggtggac gccggcgtac aacgaggcgg acgtgtccga caagccggcg
     2521 tacatccagg ccttgcccct gctgccggac ccggacggct ggccgatgga cgcctattgc
     2581 cgcgagatgt tcggcgtcga ccgattggtc gagcgggtga cagacgagct cgaggctgtc
     2641 ggccggctcg acaacacgct gctcgtcttc acagccgata acggaatggc ctggggcgcg
     2701 catcgcaggg agttcaagaa cgtgccctac gcgacggctg tgcccctgta catggcctgg
     2761 ccgggtggct ggggcaacgc tccgcgcgtg gtggacgact acgtctcgaa catcgatctc
     2821 gcgccgacct tctgcgcgct cggcggctgt gcgctgggtc catatccaag tggccagagc
     2881 gcacctgacg gcctgagcct cgtcgacctg ctcgataacg gctcgcccct gggacgggat
     2941 gccctgctgg agagcgcgct gttccaggac ccgacgaggg acgtaccgcc ctgggccgcg
     3001 gtgcgtacga cgccgcagag cgggcttggc atgtggcact acgtggagta cgccgacggc
     3061 gagcgcgagc tttacgacct ggcctctgat ccatacgagc tggacaacct cgctggcgat
     3121 ccgggccgcg ccgcgatcgc tgcctcgctc gcgcaaaggc tgggggagct actcgacgaa
     3181 ggtcgcgtaa accgtccgga cctcagcatc tggagcatga ccgacaatgc ctacgccggg
     3241 tacaacctct tcgcctcaac gcccagcccg gcccagacgc ttaccgtcag cgtggtccga
     3301 cgccggacct acacattccg gatcgacctg gctaaccaca aggtatcgac cgattcgttc
     3361 acggtgactg ccgcggtcac cggcgggcac gttcgcctga cctggctgct cgacggtgtt
     3421 gacgtgaccg gccagatgag ctctggcggc gtccgattcg agtcgcttgc gagcatgagc
     3481 agcggttctc tgacgctcca ggtgaagatc aagcggcgct tcccgagagg ccggacgacg
     3541 attgacctag tcgcccggcc ggtgagcagc cccgcacagg acgaccacgt gactttggtg
     3601 gtcaggcgct gactacatca tcaccaggcc gccgtcgacg gcgaggacct ggccggtgat
     3661 gtaggcggcc tcgtcggacg ccaggaacgc gaccgcatac gcgatctcct cgaccgtccc
     3721 gaatcggcca aggggggtgc gctcgttgat ctgcgcctta aggtcgtcgt tgaggtcctg
     3781 ggtgagctcg gtcaggacga acccgggcgc caccgcgttg accgtgatac cgcgcgagcc
     3841 cagctcgcgc gccgctgctt tggtgaggcc gatcaggccg gccttcgcag atgagtagtt
     3901 ggcctggccc atctggccgg cctggccgct aacgctggtg atgctgatga tccggccaga
     3961 cttggcgcgg agcatgggcc gcgtcacggc cttcaagctc cagaacgcgc cgaacaggtt
     4021 cgtctcgagc acgtcccgcc agtcctcggg cttcatgcgc atgatcaggt cgtcgcgcgt
     4081 aatgcccgcg ttgttgatga ggatgtcgac tttgccgaat gcgtcgaggg cggcctttac
     4141 gagcgcctcg gcggcgtccg gctcagtgac gtcagcctgc acggcaacac accggcgacc
     4201 aagcgcgtcg atctcctttt gcgtccccgc ggccgcgtcg gcgttgccgc gatacgagaa
     4261 gcagatgtcg gcaccctgcg ccgcgagacg cagcgcgatg gctttgccga tgccacgcga
     4321 tccaccagtg acgatggccg ccttgccggt cagatcaatc atgcgcagag tctaggcgtc
     4381 aaccggcacg agactgccat actggcgacc attgctccgt ccgaggggac gggagggggg
     4441 tctacatgag cgcgattcgg acgcgaacgg tgatcgtctt gaccctgtgc gcactgctcg
     4501 cgccacaggt ggcctccggc gctccgcagc cgcctccgcc ccccgacgtg cgtcccaaca
     4561 tcgtgctgat gctcatcgac gacttccccg ccatggacga gcgcgtcttc gagcggctgc
     4621 cgaacatcaa ggccctcttc ctggacaagg gcgtcaactt cacaaattac tgggccaact
     4681 tctcgctgtg ctgccccggg cgcgccgcga tcctgacggg gcaaacggcg gcgcacaacg
     4741 gcgtgatcct gaatgacgct cgcctgcttg acgccaccga gacgatcgcc acggaactgc
     4801 agggtgtcgg ctatcacacg atcatcgcag gcaaatatct gaacaagacg ggcaaattga
     4861 gcgtgaagct cccgccaggc tgggaccagg ccgccatcaa ggactccccg ccctactact
     4921 cgtacccgct gtgggtcaac ggcgtgcgcg agacacgcgg gcggtcgcca agtgactact
     4981 ccacgcgcgt aatggcgggg tacgcgcgac agttcctgga aaacgcgccc gtctacaagc
     5041 cgctatttgc atacttcgca ccgaacgcga cacatggcgg gccagacgag gacagcttca
     5101 aggactacaa gctccctgtc gtcgacccgg cccacgtcga cgacccgcgt tgcagcggca
     5161 tccccaactg gaacccggcg tcgtataacg agcccgacgt cgcggataag ccggcctaca
     5221 ttcgcaacct gggcccggtc ccgtatccca ctggatggcc gcttcggaag gcctgcgagt
     5281 cgctcctatc agtcgacgac tggctgggcg cgatagttga cgagctgcgt aacgaaggcc
     5341 gctacaacaa caccctcttt gtgctgacat cggacaacgg catgggctgg ggcgcacatc
     5401 gtctgcccgg caaggtggcg ccttacaccg cgcagatgcc cctttacgtg agctggcccg
     5461 caaagtctgg cccgacccag cgcatcgacg aaacactgct gagcaacatc gacctggcac
     5521 cgactttctg cgagctggcc cgctgcgaga tgggccccta cgccaacggg tacgccgttg
     5581 acggccaaag cttcttggga ctgatcgacg cgtcgagctc agaatcacgg cccgaacgcg
     5641 attcgatcct gattgagggc ggcggcggcg gcgtgccgcc attccgcgca atcatgacga
     5701 cggccgacca cccgctcggc cagtggttcc tgattgcgta cgtcggggtg cgcaccaagg
     5761 agctctacga tgtgagtggc ggccagtgtg tcgagtggca ggcgggcgac ccgggcgacc
     5821 cgtgcatgct cgttaacgtc gcctcgtcac ggaagtcaat ccgagccgcg ctaatgtcgg
     5881 agctgaacga cgagtggtcg gggccgctac ctgggagccg ccgcgccgaa gccccctagg
     5941 ccaagacctc gtctcggggg gcgggctccg gctcgccggg cacgacgtcg tcgagcggca
     6001 ccctgggccc ggcgttgccc tcgagcacct ggcgcagccg gtcggggatg ggatcgacag
     6061 atgaccagtc gagcggctcg cgcacctgcg caacgggctc caccgcctcc gcgcgcttgc
     6121 cgttcatcgg atcggccgtc cattcgagcg cgatgccgcc gctggtcagg cccgcgccaa
     6181 aggcgacgag cacgatcttg tcaccgatct tgatgcggcc cgaatcgacc gcctcagcca
     6241 acgcgatgcc gaccgatgcc gccgaggtgt tcccgtagcg gtcgaggttg acgaacatct
     6301 tgtccatcgg cagccccagg cccttggcga cggactcgat gatgcgcacg ttggcctggt
     6361 gcgggatgaa gagatcgatc tcttcgggct tccagccggc gcgctcgatt gccttcagcg
     6421 cggtcgtggc catggtcttg gtcgcgtagc gataggtctc gcggccttcc atgcgcacgt
     6481 agtgctcgcc gcgggcgagg gtctggcccg acgcgggtga tcgggagccg ccggaaggca
     6541 gccagatcat gtaggcaccg tccgggtcgg tcgtgagctc catgccctgc atgcccccgc
     6601 cctcttcatc ggaggctgac agcacgacgg caccggcacc gtcgccaaag aggatgcacg
     6661 tgccgcgatc ggagaagtcg aggtagcgcg acagcgtctc cgagccgacc acgagcgcat
     6721 tcttgaacat gcccgagcgc aggtacgcgt ccgcgacgga gtaggcgtag acgaggccgc
     6781 tgcaggccgc ggcgacatcc atggccgcgg cgcgcgtgtt gccgatcgcc tccttgacga
     6841 gcgcgccagt cgagggcatc cagtagtcgg gcgtgagggt cgcgaccagg atcacgtcaa
     6901 tgtcgtcggg ctggaggccc gcgacggcga tggcgcgctt gccggcgatc gcggcgaggg
     6961 tcgccgtggt ctcgtgccgg gcggcgatgc gccgctcgcg gatgccggtg cggctgacga
     7021 tccactcgtc ggaggtgtcg acgacgcgtt ccaggtcggc gttggtgagc cgccgctccg
     7081 gcgcgtacat gccccagccg gtgatgcgca cgctgcgcgg tgtcatggtt gcacctcgat
     7141 caaaggatcc ttggccttga ccagcgcgcc gtcctccggg acgacgcgca cgacgcgacc
     7201 ggcgcggtcg ctcttgatct cgttgaacag cttcatcgcc tcgatcaagc cgatgacctg
     7261 gccgaccatg atctgggtgc ccggcttgac atacggctcg gcacccggcg agggcgacgc
     7321 gtagaacagc ccggtcagcg gcgcgacgac tgcgttcatc gcgggttcct gggttggcgc
     7381 cgtcgctgat ggttgaggcg cgaccgtggc agccggtgcg gccatcgagt gaccgcccgc
     7441 tcccgcttcg gccgctgctg caaaggcacc gggcttacgc aagaccagcg ccgtctcgcc
     7501 aacctcgatc tccatctcgg tcagttcgga cgcgttgagc agcttctcga gacggtcgat
     7561 cagcccgacg atgccggcca catcggtctg cggcacgccc gcctgggctg ctcgcttggc
     7621 tccggcggcg ctcaagacgc gtcccagcgg cggaacacga gcgccgcatt ctggccgccg
     7681 aacccgaacg cgttgaccag cgccgcgcgc accgcgcgct cacgcgccac caggggcgtc
     7741 aggtcgagac cgtctgcctc aggtgcaggc tcctgcaggt tgagcgtggg cgggacgcga
     7801 ccctcgcgca tcgcctggat ggccgctatg gcgccgatgc cacccgcagc gcccagcgtg
     7861 tggccgatcg cgcccttggt cgcggtgatg ctcacgttgg gaatgtggtc gccgaagacc
     7921 gccttgaagc ccatcagctc ggccggatcg ccttccgggg tcgaggtcgc gtgggcgcta
     7981 atgtggtcga tgtcgtgggg ctgcaggccg gccttctcga ttgcccgccg cgctgctcgg
     8041 gcgcctccct ccccgcccgg tgcgggcagg gtgatgtggt tcgcgtcgcc cgtcgcgccg
     8101 tacccgacga gctcggccag gaccgtcgcg ccgcgccggc gggcgtgctc cagctcctcg
     8161 agcaccagca ccccggcgcc ctccgcgatg acgaaaccgt cgcgaccttc gtcgaacggc
     8221 cgactcgcgc cctgcggatc gtcgttgcgc gtcgacagcg cgcgcatggc tgcaaatcca
     8281 ccgacgaggg cctcgaacac ggccgcttca gagccgccgg cgaacatcat gtcggcgtcg
     8341 tcacggatga tcgtctcgta cgcctcgccg atggcgtggc cggccgtggc acaggcgctg
     8401 acggtcgccc agttggggcc gcgcgcgcca aagctgatgg cgctctggcc cgccgcgagg
     8461 ttggcgatgg ccatggggat gaagaagggc gatatccggt caggaccacg catcgcgttg
     8521 atcgtcacct gctcgaccag cgtgcccgag ccgccgatgc ccgagccgat gatgacgccc
     8581 gtgcggtcgg ccagctcacc ctcgagccgc tccggcaggc cggcctgctt catcgcctcc
     8641 gccgtggcga cgagcgcgag ctgggtgtag cggtcgttgc gccggatctc cttgcgatcg
     8701 agcacgtgcg aggggtcgaa gtccttgacc tcgccggcga tcttgctcgt caggcgctcg
     8761 ggatcgaagt tgatgcggat cgtgtcgacg ccgctctccc ccgcgacgag gcgcgaccag
     8821 aaggtcgcca cgtcgttgcc cagcggcgtc accgcaccca tgccggtgac aacgatgcgc
     8881 cggcggccgt tggtcatctg atcagccgcc cggtgcggat ggtcgcggcg cgataaacat
     8941 cgccggcaag accgcgcgcg gccacttcct cggccgccgc ggcggcgtcg tacgcgggtc
     9001 ggaacaattc ggctgacggt tgcgcatcct cgccgtcggg cagcgtgagg agcgcccagc
     9061 atgccgccgg gtcgttatcg aaggcgtagc cgcatgaccc gggattgacg atcatcttgc
     9121 ggcccagctc gcgcacatcg gccacgtggg tgtggccgca gcagatgacg cgcgcatcgg
     9181 tccgggtcac acgctccacc gtcaccgtcg ggtcgagatc tgtcggcagg cccgcgctta
     9241 tggagccggg tgagccgtgg caggccagga ggagggtgtc gccgtcccac agtcggcgct
     9301 cggacggcaa ccggcgcagg tagtcgagct gttcgtcgga gagctggtcc ttcgcccatt
     9361 cggcggcggc gatgtgggtc tgtggcacgt catcgagcca cggccaggcg gccgcatagt
     9421 cgccgtcagc cacggccagg tcggtgttgc ccgccacgac cagggcgccg cccttctcga
     9481 gcgtgcgcac gcgatcgatg acgtcaccgg gacgcggccc gttgaaggtc aggtcgccgg
     9541 tgatgacgta gcggtcgggc ttgtgcttct tcgcatcggc cagcgccgcc tcgaacgcgg
     9601 cgatgttgcc gtggatgtcg cccaggacgg cgagacgcag tgccatcagc ccagcacccc
     9661 ttcgcgcggg atgatgaacg acaggtcagc ctcgcacgcg acctcgccct cgaccttggc
     9721 caccccgtga ccgcggccaa agcggccgcc aagcttgtcc atcgtgacct cgagcgagat
     9781 gacgtcaccc ggctggacga cgcgcttgaa acggcactca tcgatcgccg cgaacaaccc
     9841 gatccgatcg ccaaagcctt cctgctgcgc gacgtagacg gccatcgtct gcgccagcgc
     9901 ctccacctgc aggacgccgg gcatgaccgg cagctccggg aagtggccct ggaagaacca
     9961 ctccgtggcc gtgaccgcct tctcgccgcg gatgtagccg cgggccgggt cgtattcgac
    10021 gatccgatcg accatcagga tgggccagcg gtgcgggatc agcttctgga tctgcgctgt
    10081 tgtcaggacg atttggctca aattgcttct ccgtcaggcg gccgcgtcgg ctgcggccat
    10141 gaaggctgcg ctcccgacgc gatcgagcag gttggtcgtg aactccccat tcaggaatgc
    10201 ctcgttggca agcagcgcgc gatgaagcgg cacgttggtg accacgccat ccacgacgag
    10261 ctcgtcgagc gcggcacgag aacgggcgat ggccgtcgca cggtccggcc cccagacgat
    10321 gagcttgccc aggagcgagt catagtaggg cggcacgtca tagccgctgt aaagatggct
    10381 gtccatgcgc acgcccgggc cgccgggcgc gaggtaggtc tcgatccgtc ccgcggcggg
    10441 ccgaaagtcg tgcgccacat cctcggcgtt gatgcggaat tcgatcgcgt ggcccttgag
    10501 ctcgatatcc gactgcttga cgctgagcgg ctcgccggcg gcgatgcgta tctgctcggc
    10561 cacgatgtcg aggcccgaga tcatctcggt caccgggtgc tcgacctgga tccggcagtt
    10621 gatctcgatg aagtaccagt tgccctggcg gtcgagcagg aactcgagcg tgcccacgtt
    10681 ctcgtagccc gcggcgacga ccgccttgat cgcggcgtca agcaatgccg cgcgctgctc
    10741 ggcgttgagc gccgggctgg gcgcctcctc gaggagcttc tggtggcggc gttgaacaga
    10801 gcagtcgcgc tcgccgacgt ggacgccgtt gccgaagcgg tcgaccatga cctggacctc
    10861 gacgtggcgg ttgtcttcca gccacttctc gaggtagagc gaatcgtcac cgaacgcggc
    10921 aagcgcctcg gagcggcaga tggtcatggc ctgctgcagc tcgcgtggcg agcggaccat
    10981 gcgcatgcct ttgccgccgc caccggccga tggcttgatc agcaccgggt agccgatctt
    11041 gtcggcctcg gccatgccgt gggcgtcgtc gcgcaacatc cctgaaccgg ggatggtcgg
    11101 caggccgtgc ttggccagca gtgtccgcgt cccggccttc gaggcaaacc tttcgagcac
    11161 ctccggcgac gggccgatga acgtcaggcc gtgggcacgt gtcatctccg cgaacgtgtc
    11221 gtcctcggag aggaagccgt agcccgggtg gatcgcctcg caaccagcga cgatggccgc
    11281 gctcaggatc gacggcgcgg ataggtagga gcgccgtgac tcggcgggac ccacgcagat
    11341 cgcctcgtcg gccagctgca cggcgcgcga ctcgcggtcg gcttcgctgt atgccacgac
    11401 gctggtgatg cccatgctgc ggcaggcgcg cacgatgcgc agcgcgatct cgccccgatt
    11461 ggcgatcagg atccgactaa acatttcctt ccacccgacc gatggcctgg ccgaactcga
    11521 ccgcctgacc ggattcgacc tcgatctgct tgagcacacc gtcgatgggg ctgatcacat
    11581 cctggcgcac gccgagcatg tcgaccacgc cgatcagatc gcccttgcgc aggcggccgc
    11641 cgaccgcgac gccgtcgcgc ggcgtgaagt agccaacagc tggtgacgag acctggccgc
    11701 gcacgggctc acgccgcggc tgagcgtcag cggcgtgggc gaccgcggcc ggcttatggt
    11761 gctgcgtctg acccgtggca tgccctgctg ctgcgacgac atcgcccgac ccgcggcgca
    11821 gccggacgcg ccagccgtcc tggcgcacct cgatctcgcc cagatcgctc ctggtcaggc
    11881 gctcgatcag cgtcggcacg acttcgtcgg tcaggcgcgc gatcgtggcc cgctcgtcgg
    11941 tgcgcgtttc gttgcttccg gtcacgggct acatgccctc cccggtgcga gtcgactgat
    12001 ctggcgtgtc gtccggctcg gccggcgctg cggcctcacc gccgaccagg gccgcgcggc
    12061 cgctgtcgag caggcgccgt atgcgcgcca cgatgcccgg ccgctccggc tgcgcctcgc
    12121 ccgtctcggc cgcgaccgcg aactcgccca tgttgcgata gcgcgcatag cgcccgtcga
    12181 gcagcgcctt gcggtcgcgc ccggcgagcg agtcgaggtg ggcggcgatg cgcgtgcgca
    12241 ggcgtttcgc cgtctcgtcg gggtccgcgt gcgcgccgtt ggccggctcg ggcacgacct
    12301 cgtcgatcac gcccagggca tgctgctcag cagcggtcag gcgcatggcc atcgccgcct
    12361 gctggctgaa ctcggccgag cgccacagga tgctcgccgc gccctcaggt gagatgaccg
    12421 agtagatcga gttctccagc gccagcacca cgtcgcccac ggccagcgcc agcgcgcccc
    12481 ccgacccgcc ctcgccggta atgaccgtga cgatcggtgt ccggaggccg gtcatgacca
    12541 tgatcgcgcg ggcaatcgcc tccgccacgc cgcgctcctc ggacgcaggc ccggggaaag
    12601 cgccgggcgt gtcgatgaac gtcacgactg gcaggcccag ccgctcggcc aggcggaaca
    12661 gccggatcgc cttgcgatat ccctccggat ggggcatacc gaagttgcgc cgaatgttct
    12721 cgtcggtctc cgcgcccttc tggtggccca caaagacggc gcgccggccg tcgatccggc
    12781 agaagccgcc gacgatcgcc ggatcgtcgc ggaacagccg gtcgccgtgc agctcgatga
    12841 cctcgctggc gatggcgtgg accagctcga gcgtgtgcgg ccgcttgacg ttgcgcgcct
    12901 gctggacgcg ctgccagacc gcggcacgca gctcgtcggg cgtcgcctcc accggtggcg
    12961 cctgggtggc agtgcgctca gccacgggcc ggctcgcgac gaatgagctc gtcgagcagc
    13021 tgcgccgagc ggcccagtgc tgacgagccg ttcaagccag cccgttccac gtgggcattg
    13081 ggcttcatgt agcgcagcaa acccaggacc gtctcgcgca gcttggagcg ctggacaacc
    13141 atgtcgacga agccgtgctc gtacaggaac tcggcgcgct ggaagccctc cgggagctcc
    13201 tcggcgatcg tgcccgcggc cacgcgcgcg ccggcgaagc cgatgagcgc cttgggttcg
    13261 gcgatgttga catcgcccag cgctgagaac gaggcgaaca cgccgcccgt cgtggggtcg
    13321 gtcaggatgc tgatgaacgg cacgccggca tcgcccagcc ggcccagcgc cgccaccgtc
    13381 tttgcgagct gcatcagcga cagcgtgccc tcctgcatac gcgcgccgcc cgaagaggaa
    13441 acgatgatca gcggcagccg ctcctcgagc gcgtgctcgg cagcgcgggt gaccttctcg
    13501 cccacgaccg tgcccatgga gccacccatg aaggcgaagt ccatgatgca caccacgacc
    13561 cgtgtgccgg cgatcgtggc cgtgccccat gcagctgcgt cgcgcatgcc ggtggtggcg
    13621 cgcgcggcgg ccaatcgttc ggggtagggc ttctggtcga cgaagcccag catgtcgact
    13681 gattcgaggt tcgcgtcctg ctcgcggaaa ctgtcgcggt cgagcagctg cgcgatgcgc
    13741 gcgcccaggc gcagccggaa gtggtggccg cacttggtgc acacgtgctg gttcttgtcg
    13801 agctgcttgt tgtagagcat ctcgtcgcag ctgggacact tggtccacag gttgggcggg
    13861 tactcctgct tgggccggcc gaacggcagc ttgaagccgg gcatggctag tcctggcctt
    13921 catcggcgcg cggccggtgg cgccacgccc acatcgctgc gccggcgagc gacacacctg
    13981 atgcgatggc gccgccgatc acgacgcgcc gctcacgcag gaccagcggc gcgaccgggg
    14041 tgccggcatg gcgctcttcg gccagcgcgc gcaactgctc ggccaggcgc tcctcgaaga
    14101 ggaagctcgg gtggaaacgg ggcatgccgc gctcgagggt gcgaatcacg cggcgcacgc
    14161 tgggcgcggg cgaggcatgg gcgaccggca gcgcgaccgg cagccgcgcg tggccgctca
    14221 gcaatgcgtc gatgtaggcg tcgctctgga tcgccccttc ttctgcttcg cggaacatca
    14281 gttcgtcgcg cgcctgcgct gcgctgcgcg acctttctcg ctgccccggc tgcgcccgat
    14341 cgagcgcgcc acggcctgca gggagcggtg gatcagcacg cgcgtcgcgc cctccgagcg
    14401 gccgagcacc tggccgatct cgcgcgcgtc gagctcgttg acgaagcgca gtacgagggc
    14461 gcggcgacgg tcgggcggca acaagtcaat ggccgcgagc gcggcacgca gctcagcgcg
    14521 ctgagccgcc gcgtcctgaa cgttgtccgc ggcgggcaga tccagggcgg catcgagcgg
    14581 cgcctccgga tgacggcggc tgcgccggcg ctcgttcgac aatgcgttgc gcgcgatctg
    14641 gaatagccac acgcggaagc tggactgcgg cggttcagcc tgctcgtgga agtggcgcag
    14701 gccgcccagc gcgcgcagaa agacctgcgc ggtcaggtcc tcggcagcgt gcgggtcgcg
    14761 cagctcgtag agcgcgaagt tgtacacctg ggcgacgtac ttccgataga gcgcctcgaa
    14821 ggcgcgcggg tcgcgccggg ccgcctctac ggcggtgcgg tcggcgtcga gatcaaccag
    14881 ggccgcgacg cggtccttcc ctctccttaa caccggtgaa gtttagccgt tacaccaagc
    14941 caaatcccct taaaggcgat ttccaggcag ggcactggac acctgtggcc gtaccatccc
    15001 gcgaatgcgc cactaccacg gcttcatcgc cgacagcgac cgctggcagc gcttcaagat
    15061 gcgcgatgac gacgtggtga tcaccacgcc gtcgaagtcg ggcacgactt ggatgcagac
    15121 catcgtcggc atgctgctgc gcggcacgac cgacctgccg ccgatcagca cgatctcgcc
    15181 ctggatcgac atgcagatcc gcaccgagga ggagttgttc cgggtgctgg acgcgcagac
    15241 gtggcggcgc ttcgtcaaga cgcacacgcc gttcgacggg ctgccctaca acccgcacgt
    15301 cacgtacatc gcggtcatcc gccatccgct cgatgtcgcg ctgtctgatc gcgaccatgg
    15361 gctcaatatg aaggaggacg agaccgtcgc gctgcgcgag gccgtggccg ggaagttcga
    15421 gaggcccgcc caatgggagg aaccgccgga cgatccggca tcgttcctgc gctggttcat
    15481 cgataacagc cgtccgccca ccggcagcgg gcccaacggc ctcgacgatt actgccagca
    15541 ggtgcgcacg tactgggatg tccgtgaccg gcccaacttg catctctttc attaccagga
    15601 catgtggaac gacctcgatg gcgagatgcg gcgcgtcgcg gcggcgctgg gcgtcgcgat
    15661 cgacgaggcg acatggccgg gctacgtgga ggccgccacc ctcaaatcga tgcgcggccg
    15721 cgcggcgaag gcggcgccag acgggcacct cgacatctgg aagtcgcacg aggacttctt
    15781 ccgcgccggc ggcacgcgcg actgggcgca gctattgaca cccgccgagc tcgcccactt
    15841 tgatgatcgc ctacgcgacc tcgcgggtga cgcctacgac tggatcatgc gcggcaaggc
    15901 cgccttgccc ggcaaggccc caacggcggc acacgactag gtgatccggc agcgcgtcac
    15961 ttcaccactg tcgatgctca tcagcgtctt ttcgtcccgc gccagcagca gggcgccgct
    16021 ctccggatcg acgccggtcg ctctgcccag gatcgattcg ccgccgacct cgacgacgac
    16081 ctggcggccg gtcgtgatct gggtggccgt ccagccggcg gcatcgaacg tgcccgcgtt
    16141 cagcgcctcg tagcgcggct cgaggcgggc caggaaggcg tcgagaagcg cagcatggtc
    16201 gatcggccgg ttgcccgaca gctggcgcag gctggtcatg gtcgcgccca gcagccatgg
    16261 gaagtcggcc gccttccagt tgccgttgac gccgatgccg atgacggccg attggatacg
    16321 ccgcgcggtg ctgaccgatt cgcccagcac gcccccgacc ttgaccactc cgcggcgctc
    16381 atcggcggcg acgagatcgt tgggccactt gagccagatg gtgccgtcgg ccaggccggc
    16441 cgcctcctct gccgcgtcgc gcatcgcgag cgccgcagtc gcggctagcc gccagccgtg
    16501 gcgcggcgcc aggacctggg gccggaaccc agccgacagt agaagcgccg agccagccgg
    16561 cgcgatccag tcgcggccca ggcggccgcg gcccgccgtc tggtgatcgg cgaccgcgac
    16621 acacacctct ggctcgccgg cctcgagcca ttcacggaca acgcgctgcg tggagtcgac
    16681 ggcgcgaaag tgctcgagtc ggctgggaaa ggtcacggcg cttggtcggt taggtggcgc
    16741 gcgccggtgg ccgtcgggcg gacgacagtc gcacggccgc cgacgccggc gctcgccgcg
    16801 tacgcctcca tggccgcggc cacatctcgt gctgccgccg cgccatcgca tagcgcgata
    16861 accgtcgagc cggcaccgga cagcgccgcg ccgagcgccc cggcatcccg cgcagcggcg
    16921 atcaatccgg ggagcgcgac gaaatgggcc gcgcgatgcg g
//
LOCUS       DATBRX010000191         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1303882, whole genome shotgun sequence.
ACCESSION   DATBRX010000191 DATBRX010000000
VERSION     DATBRX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303882"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..768
                     /locus_tag="VIF08_07445"
     CDS             88..768
                     /locus_tag="VIF08_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8135861.1"
                     /translation="MVDATPSESLSGVEPAAEPAEAVEAPDAAPTKPNDSLQHARDRA
                     LVERAITGDLDAFNEIVLLYQDYLFALVVRVVHDRDSAADAVQEALFSCYRNLDRFRG
                     DSFRSWLTRIALNAATDVLRYRKRRPADPYPEWEDDSWQPPAGEEASPERRTMRRQTT
                     LALREALAEITSDQRTAIVMYDVEGFDYQEIADITGVSLGTVKSRIHRGRLALRELLS
                     EDMELLRE"
     gene            784..1545
                     /locus_tag="VIF08_07450"
     CDS             784..1545
                     /locus_tag="VIF08_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135862.1"
                     /translation="MPNTHSLSAEHRRHDRLLVARFAAGDAAFGQEHEAQNLVRRCSE
                     CAALVADISAISKSVARMPAPPRPRDFRLTAEQAAHLRGSRIDRWLRTITGSGWSTVR
                     PVAAVALSIGMVMSVVGFLPVIGAASGGPTATDAAQVPVAADQPTPPMERSTDAHAPG
                     ATPNTTDVGPDGKVDVSSAESPADNLDNAYLVQETAAPQGDGQPNDLVAPLPGTGSGV
                     RDLVLLAGVAVTIAALVVLALLYAARRRYYDPLLR"
     gene            complement(1524..>1828)
                     /locus_tag="VIF08_07455"
     CDS             complement(1524..>1828)
                     /locus_tag="VIF08_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135863.1"
                     /translation="GFATGTDGPPIGPPGVDLPPNPVDVYFGGGLLGYLVTSQLPLLI
                     GAAMLGHWYGTRTRLQAYLGGLLREVSPEDRETIAEMTYDAARARSAAGDQRSSGS"
BASE COUNT          288 a          659 c          593 g          288 t
ORIGIN      
        1 gctgacacac agcgggtcgc ccaaccgtcc gatgctagac tcgggaccag ttcccggcgc
       61 cggagggact acgcatccgt gagggaaatg gtcgacgcga cgccgtctga gtcactgtct
      121 ggcgtcgagc ctgcggcgga gccggccgag gccgttgagg ccccagacgc agcacccacc
      181 aaacctaacg actcgctcca gcacgcccgc gatagggcgc tcgtcgagcg cgcgatcacg
      241 ggcgacctcg atgcgttcaa cgagatcgtg ctgctgtacc aggactacct cttcgcgctg
      301 gtggtgcgcg tcgtgcacga ccgcgactcc gcggccgacg ccgtgcagga agcgttgttc
      361 tcctgttacc gcaacctcga tcgctttcgc ggtgattctt tccgttcgtg gctgacgcgc
      421 atcgcgctca acgccgcgac ggacgtgctg cgttaccgca agcgccgccc ggccgacccg
      481 taccccgagt gggaagacga ctcctggcag ccgccggccg gagaagaggc gtcgcccgag
      541 cgacggacca tgcgccgtca gaccaccctc gcgctacgcg aagcgctggc tgagatcacc
      601 tccgaccagc gcacggccat cgtgatgtac gacgtggagg gcttcgacta ccaggagatc
      661 gccgacatta ccggcgtgtc gctcggcacg gtcaagtcgc gcatccatcg cggccggctg
      721 gcgctgcgtg agctgctcag cgaagacatg gaactcctga gagagtagag gcgtcagaag
      781 accgtgccca acacacattc actctccgct gagcatcgcc gccacgaccg cctcctggta
      841 gcgcgtttcg ccgcgggcga tgccgctttc ggccaggagc acgaggcgca gaacctggtc
      901 cggcgctgct ccgagtgcgc cgcgctggtc gccgacattt ccgcaatctc gaagtccgtt
      961 gccaggatgc ctgccccgcc ccgcccgcgc gacttccgcc tcacggccga gcaggccgct
     1021 catcttcgtg gctcgcgcat cgatcgctgg ctgcgcacga tcaccggctc cggctggtcg
     1081 acggttcgac cggtcgcggc agtcgcactg tccattggca tggtcatgag cgtggtgggc
     1141 ttcctgcccg tgatcggagc cgccagcggc ggacctaccg ccactgatgc cgcgcaggtt
     1201 ccggtcgccg ccgaccagcc gacacccccg atggagcgca gcactgacgc gcacgctccg
     1261 ggcgcgaccc ccaacacgac agatgtcggc cctgacggca aggtcgacgt cagctcggcg
     1321 gagtcgccgg cggacaatct cgataacgca tatctggtcc aggaaaccgc cgccccgcag
     1381 ggcgacggcc agcccaacga ccttgtcgcg cctctccccg gtacgggttc gggcgtgcgc
     1441 gacctggtcc tgctggcggg cgtggccgtg acgatcgctg ccctcgtggt cctggcgctg
     1501 ctgtacgccg cgcgccggcg ctattacgac ccgctgctcc gctgatcccc agctgcgctg
     1561 cgcgcccgcg ctgcgtcata ggtcatttca gcgatcgtct cgcggtcctc ggggctgact
     1621 tcgcgcagca agccacccaa gtaggcctgt agtcgcgttc gcgtgccgta ccagtggccc
     1681 agcatcgcgg ccccgatcag gagcggtagc tggctggtga ccaggtagcc caacaggccg
     1741 ccgccgaagt acacgtcgac cggattgggt ggcagatcaa cgccgggcgg gccgattggc
     1801 ggtccatcgg tcccggtcgc aaagccga
//
LOCUS       DATBRX010000192        13933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1082013, whole genome shotgun sequence.
ACCESSION   DATBRX010000192 DATBRX010000000
VERSION     DATBRX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082013"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..965)
                     /locus_tag="VIF08_07460"
     CDS             complement(174..965)
                     /locus_tag="VIF08_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEY8135864.1"
                     /translation="MRVLMIGDVIGKPGRVAVERLLPKLRDERDIQLVTANGENMAGG
                     MGLTPSTAQSLFDAGVDVITSGNHIWDKKEIYPELDRDERILRPINYGEQGVPGRGWG
                     VFHAHDGTEIAVINAQGRTYMAQIENPFTMLDALLDQGAAELPALRIVDFHCELTSEK
                     NAFGIYLDGRVSAVCGTHTHVATADERVLPKGTGYVSDIGMTGPLHSVIGFDPATVLP
                     RFINALPTRFEVGSGPILFNAIQLDLDPASGHAVAIERIREVVEE"
     gene            complement(971..2503)
                     /gene="rny"
                     /locus_tag="VIF08_07465"
     CDS             complement(971..2503)
                     /gene="rny"
                     /locus_tag="VIF08_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY8135865.1"
                     /translation="MNDPVVLAVVAIVAIAAGFGVGFFARSMWASTAIRTAQDKSARI
                     VAEARAQQKELILQAKDEQVRLAREGEEEARARRNELASQEGRLRQRDEQLDQRGDML
                     AERDRKLLEREREFDTTREELARAREEQIRALEKVSQMSQEDAKLVLLEQVRVDAEHD
                     AVRLARSIERKAKEEADEKARDVLVTAIQRVMVDHTAEMTVTTVQLPSDEMKGRIIGR
                     EGRNIRALEQATGIDLIIDDTPETVLISGFDPVRREIARLSLTKLIQDGRIHPGRIEE
                     VVAKAKAEVELVMRQAGEAAAYDVGVPGLPAELIKLLGRLKFRTSYGQNVLQHSIETA
                     RLAAAIAQETGADAQIAKLGGLLHDMGKAVDHEIEGPHAQIGATIAQRHNLPMKVINA
                     IAAHHQEVEYACVEAPIVQIADAISASRPGARGESAETYLKRLEELQAIANSFEGVEK
                     SFAVQAGREVRILVRPEEIDDLAQARLARDIVRKIEESLTYPGQIKVTVIRETRAVEY
                     AK"
     gene            complement(2774..3466)
                     /locus_tag="VIF08_07470"
     CDS             complement(2774..3466)
                     /locus_tag="VIF08_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135866.1"
                     /translation="MAKKEKPPAGPDDLVRAGAGAYTSGDGRFAVQKSDQRWFLVDNE
                     QSNEFGQQLIHGPFDTLEDVRAAIPGAREIKPLLRVPRRVTPAPAKSGTGRGGKSSAT
                     QKAASRKPPTPMSWIDRLPAREAAEVRRLIAVLEAEGISSAESVVKRDRDSEGFALIA
                     EEIIARRLAKVVAGATPAARDELRRAVRRVAEILTDEGGATARPLPRWELVETPRDPG
                     QPPRRIRPRVGP"
     gene            complement(3485..5932)
                     /locus_tag="VIF08_07475"
     CDS             complement(3485..5932)
                     /locus_tag="VIF08_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY8135867.1"
                     /translation="MAREGLSAAEVVARRQRGLGNDAREKGGRTYLQIFVENTFTFTN
                     NIMFLLGLGLVIVGRPLDALVSVLVVGTNVVVGVVQEVRAKRTLDRISLLTRPTASVI
                     RDGTTTTVNPRELVVGDLIRVQAGDQFVLDGRLVDGRLQADESLLTGESDLVRKGTGD
                     EVFSGSFCATGSGRYVVERVGTDSLANRITQGAKAFRRVLTPLQRSVNSVIRLALVLV
                     LYMELLLLLTAIIHVAAPGETIAQASVLAGLIPNGLFVSIAVAYALGAVRLVRFGALV
                     QQANAVESLSRVDILCTDKTGTLTANRLALRELVPITSDEAGLRRSLGTLVASTTERN
                     KTAEAIATSLPGDAASVQREVPFSSARKWSGATMTDGNSYVLGAPQLLKPALGEAHSA
                     RWPGIEAQVNERAASGLRVLILLTSNDPNAITGDDDDARLTATFAPLGLVVLEDELRP
                     GVGPILERFRQAGVQVKIISGDDPDTVVALARQAGLTGDLPHVSGTQLDDLDDTELAA
                     VARRNVVFGRITPTQKERLVASLRAGGSYVAMIGDGVNDVLSLKKSNLAVAMASGSQA
                     SRGVADIVLTEDSFAALAPAVEEGQRIVNGMFDIISLFLARIATMGLVIVSSLVVGIF
                     PIALRNASAITLLSVGIPAALLALWAQPGKVQHQSLTRMVIRFVLPAALLSSAAALAV
                     FYGSIWLSVSALPAAAGTALQEAAVINATPAAQSALTAFLVFVGLGLIVFVEPPTNWL
                     AVTQERSSDRRPTVLAIVLGLVFVILTLIRPLGDLFALAPLDLVQWALVLGAFALWFV
                     VVRQTWHWRLFERFVGA"
     gene            complement(5954..6409)
                     /locus_tag="VIF08_07480"
     CDS             complement(5954..6409)
                     /locus_tag="VIF08_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY8135868.1"
                     /translation="MRIDRLALALLLAYLVVLAVLTLRPVGADVESNVRVNLRPLATI
                     LPALRHGPGTFAFAILIGNVLAFVPFGLLVPLAVRRIPWLLVLVAGTALSATIELTQY
                     ALSTNLGYGYRRADIDDVIVNVTGTLVGLALYALIKLAANLVRWVRSAG"
     gene            complement(6396..6977)
                     /locus_tag="VIF08_07485"
     CDS             complement(6396..6977)
                     /locus_tag="VIF08_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135869.1"
                     /translation="MKRALTLALAVALVGAAFVAFVVNPLVPGNDFAVDQAHLDPSGD
                     FFAYAPSTDYYVMTTMKNSGLLPISVNGLETNTDPAYLGAYAVEFRLAHGVSETANMA
                     ALSDSEPFHPVDVEPGDVLAVWVHFRTGPCMLDGTLPYPPGSGTGLDDIAVKWSFFGV
                     PRVSAVPLVMDLLVLNVENDAPDPCGEALVEDR"
     gene            complement(6974..7513)
                     /locus_tag="VIF08_07490"
     CDS             complement(6974..7513)
                     /locus_tag="VIF08_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEY8135870.1"
                     /translation="MIRRRSTPESVKARREARGELTEVGPVIEAAAAFVAVRPRSVDE
                     TRRRLQQLGYPQGLIGQVIERFAEVGYLDDESFARTWVESRDRARPRGESVLRRELAQ
                     KGVGRQVVDEVLGERLDAADGDDPNLAAATALLERKRAALLREPDERKRRQKAYALLA
                     RNGFDPETCRVVASSFVAT"
     gene            complement(7800..8879)
                     /gene="recA"
                     /locus_tag="VIF08_07495"
     CDS             complement(7800..8879)
                     /gene="recA"
                     /locus_tag="VIF08_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY8135871.1"
                     /translation="MAMTGTKSTAESNGVEKAEKPSERTRAVDAAILAIEKQFGRGSI
                     MKLGTAERQAVDAIPTGSIALDLAVGVGGIPRGRITEIFGPESSGKTTLCQHVLAEAQ
                     KRGGVVAFIDVEHALDPGYARACGVNVDELLVSQPDTGEQALEITETLIRSGGVDCVV
                     VDSVAALVPRAEIEGEMGDSFMGIQARLMSQALRKLTGAVSRSNTALVFTNQLREKIG
                     VMFGNPETTPGGRALKFYASVRLDIRRIETIKSGTDSIGSRVRVKVVKNKVAAPFRVA
                     EFDVMYGEGISREGGLLDVGVASDTVSKTGAWFNYGDLRLGQGREQAKEFLKANQDVA
                     QKLETEIRAKVATITVPVEGISEAE"
     gene            complement(9169..10350)
                     /gene="tgt"
                     /locus_tag="VIF08_07500"
     CDS             complement(9169..10350)
                     /gene="tgt"
                     /locus_tag="VIF08_07500"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY8135872.1"
                     /translation="MTVELDTNPVSRPMSFDVLLPSPADGSTRARLGHITTPHGEIQT
                     PVFMPVGTNATVKALDPDDLDEIGAQIILCNTYHLYLRPGHERIARLGGLHKFMSWQR
                     PILTDSGGYQVVSLGDFRSVDDDGVTFRSHLDGSIHKFSPEHSIAVQEALGSDIAVVL
                     DQPVPPHASSRREVAEAMVRTHSWAERCLSSHKRPDQALFGIIQGGLEPDLRAESTQV
                     IAGMAFDGLCIGGLAGDESAAQRRETLDVVVPLLADDPRPRYLMGLGSPADLLDAVDA
                     GIDMFDSVLPSRIARNGTVWTPTGRLNLRNSRFLDDPAPLQEGCRCRACRSFSRAYLA
                     HLLRAEELLFYRLATLHNLTYLADFMARIRQALAQGKFPKLRAELAVPEPPPSIWDEQ
                     A"
     gene            complement(10350..11258)
                     /locus_tag="VIF08_07505"
     CDS             complement(10350..11258)
                     /locus_tag="VIF08_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135873.1"
                     /translation="MRLIRAELLKARRRTATWVLLVVALALTALIYFLIGRSIGGVGC
                     DITIGDFGQSCIVEFPAAYALIDQFAFTTLGGLLAIVYAAAFVGADWNWGVLRNVIAR
                     GESRARYLLAKAAGLSIVLLVGCAIIFFFALAMTFVSALSQGIPIASPLRGNGLLDLV
                     TNFALGFVVVLERAALGFAVAVVLRSQLAGAVVGIVLYLGEGIVTTYLTFSAAARFFG
                     GNGGGIGEGDLQLIGPEWFQYLPMTVGNQVLSWAPGVAGNIAAGGIQGIFLRPVPFEQ
                     AFPALLIYLFAAVAVAVVSLNRQEII"
     gene            complement(11255..12343)
                     /locus_tag="VIF08_07510"
     CDS             complement(11255..12343)
                     /locus_tag="VIF08_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135874.1"
                     /translation="MTGDIQPAPRPEFLPRSDAPTPDLAVATRGLTKAYGRQVALTGL
                     DMSVPTGRVYGFLGPNGSGKTTALRLLTGLIRPNAGTISLFGTPYTWHDRTRLFRVGS
                     LIETPSFYPYLSGAENLLVLGASGPPTDPARIDRVLEYVGMSGRAKSPVQTYSLGMKQ
                     RLGIAAAMLSDPDLLLLDEPANGLDPGGMVAMRETLRYFTAELGKTVIISSHLLHEVE
                     MLADVVGIIDHGRLIREGPLAELLESAGRVRVRVAPEDMTKAAAVLRGLAPDRPLYGI
                     DSGEGAGWFTVGIAPARAAELNRVLAAAGVYASGLEPGSDLESIFLELTGRTGNVAAP
                     PASTGPDAAPALSALSPADPGPPSMGAA"
     gene            complement(12340..13443)
                     /gene="ruvB"
                     /locus_tag="VIF08_07515"
     CDS             complement(12340..13443)
                     /gene="ruvB"
                     /locus_tag="VIF08_07515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY8135875.1"
                     /translation="MQEPGLEPDRPVVTGTLSVEEVAVETSLRPRLLAEYIGQGTVKQ
                     NLEILLAAARQREEAADHVLLYGPPGLGKTTLANIIARELGVNLHATSGPAIERPGDL
                     AAILTNLDERDVLFIDEIHRLNHAVEEILYPAMEDFAIDVMIGRGPSARSLRLSIKPF
                     TVVGATTRAGRIGGPLRDRFGAVYRLDYYSQDELATIVQRSAGILRVDIEPDAIQVIA
                     RRGRGTPRVVNRLLRRVRDYAQVRSSGHIGAAEATAAMNAMDIDDEGLDTTDRRLLAA
                     IVQKFASGPVGGAAVAAVIGEEVETIEDVYEPYLLQLGFLDRTPQGRVATERARDHLR
                     GLGYEVPAPRKADRADSGLWDGITHEGRAEVEA"
     gene            complement(13518..>13933)
                     /locus_tag="VIF08_07520"
     CDS             complement(13518..>13933)
                     /locus_tag="VIF08_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEY8135876.1"
                     /translation="FVAPFDREDIHELTVRLDDVVDLIQAVGESLSIYDVKAPTEDCR
                     RLAQILALQGAELAKALGKLNKMKDIGEHLAKVHELEHEADGVSRAAIGKLFRDSTDA
                     LEVIKWRELYLALENAIDAGEDAAEAIERMIHKRV"
BASE COUNT         2217 a         4774 c         4661 g         2281 t
ORIGIN      
        1 ggcacagatc tcgagcccga cctcggtcat gtgctggtag agctcggtcg ggggcaggac
       61 gccgtcggat cggtcggagt gcgagtgcag gtcgaccttg ttggccggcc aacccggctg
      121 aacgacgggc tcggcgaagc gactctggac cgcgggcgcg gccgtctcgc cggtcactcc
      181 tcgaccacct cgcggatgcg ctcaatggcg accgcgtggc ccgaggcagg atcgagatcg
      241 agttggatcg cgttgaacag gatgggccca ctccccacct cgaaccgcgt cggcagcgcg
      301 ttgatgaagc gcggcaggac ggtcgccgga tcgaagccga tcacactgtg cagcggcccg
      361 gtcatgccga tgtcgctgac atagcccgtg cccttgggta gcacacgctc gtcggcggtc
      421 gcgacatggg tgtgcgtccc gcacaccgcg ctcacgcggc cgtcgagata gatgccgaac
      481 gcgttcttct cgctcgtcag ctcgcagtgg aagtcgacga tgcgcagcgc cggcagctca
      541 gcggcgccct gatcgagcag cgcgtcgagc atcgtgaacg ggttctcgat ctgggccatg
      601 taagtgcggc cctgggcgtt gatcaccgcg atctccgtgc cgtcgtgggc gtggaaaacg
      661 ccccagccgc ggccgggcac gccctgctcg ccgtagttga tcgggcgcag gatgcgttcg
      721 tcacggtcca gctcggggta gatctccttc ttgtcccaga tgtggttgcc gctcgtgatg
      781 acgtccacgc cagcgtcaaa gagcgactgc gctgtggacg gcgtgaggcc catgccgccg
      841 gccatgttct cgccgttggc cgtgacgagc tggatgtcgc gttcgtcgcg caacttgggc
      901 agcaggcgtt caaccgcgac gcgaccgggc ttgccgatca cgtcgccgat catcagcacg
      961 cgcatggcgg ctacttggcg tactccacgg cgcgcgtctc gcggatgacc gtcaccttga
     1021 tctggccggg gtaggtcagc gattcctcga tcttgcgcac gatgtcgcgt gccagccgcg
     1081 cttgggcgag gtcgtcgatc tcctcggggc gaaccaggat gcgcacctcg cggcccgcct
     1141 ggacggcgaa ggacttctcg acaccctcga aggagttggc aatcgcctgc agctcctcga
     1201 gacgcttgag ataggtctct gccgactccc cccgggcgcc agggcgcgac gcgctgatgg
     1261 cgtcggcgat ctggacgatg ggcgcctcga cgcaggcgta ctcgacctcc tggtggtgcg
     1321 cggcgatggc gttgattacc ttcatcggca ggttgtggcg ctgcgcgatc gtggcgccga
     1381 tctgggcatg cggcccctcg atctcgtggt cgaccgcctt gcccatgtcg tgcagcaggc
     1441 cgcccagctt ggcgatctgc gcgtcggcgc ccgtctcctg ggcgatggcc gccgcaagac
     1501 gcgccgtctc gatcgagtgc tggagcacgt tctggccgta cgacgtgcgg aacttgagcc
     1561 ggccgagaag cttgatcagc tccgccggca accctggcac gcctacgtca tacgcggccg
     1621 cctcaccggc ctggcgcatg acgagctcca cctctgcctt ggccttggcc acgacctcct
     1681 cgatgcgccc tgggtggatg cgtccgtcct ggatgagctt ggtcaggctc aggcgcgcga
     1741 tctcgcgccg gaccgggtcg aagccgctga tgagcaccgt ctccggcgtg tcgtcgatga
     1801 tcaggtcgat cccggtcgcc tgctcgaggg cgcggatgtt gcgtccctcg cgaccgatga
     1861 tgcggccctt catctcgtcg ctcggcagct ggacggtcgt gaccgtcatc tccgccgtgt
     1921 ggtcgaccat gacgcgctgg attgccgtga cgagcacgtc gcgtgccttc tcgtcggcct
     1981 cttccttggc cttgcgctcg atgctccgcg ccagccgcac ggcgtcgtgc tcggcgtcca
     2041 cgcggacctg ctcgagcaag accagcttgg cgtcctcctg cgacatctgg ctgaccttct
     2101 cgagcgcgcg gatctgctcc tcgcgcgcgc gggccagctc ctcgcgcgtc gtgtcgaact
     2161 cgcgctcacg ctccagcaac ttgcgatcgc gctctgcgag catgtcgccg cgctggtcga
     2221 gctgctcgtc acgctggcgc aggcggccct cctggctggc gagctcgttg cggcgggctc
     2281 gggcctcctc ctccccttcc cgggcgagtc ggacctgttc gtccttggcc tggagaatca
     2341 gctccttctg ctgcgcccgt gcctcggcca cgatgcgcgc cgacttgtcc tgggcggtgc
     2401 ggattgcggt ggacgcccac atgctgcggg caaagaaacc gacgccaaag ccggcagcga
     2461 tggcaacgat agccacgacc gccagcacga cgggatcgtt catgtcctct tcctcccgtc
     2521 cggttttgcg accggaagga tgtatttgct tgttgttgtc gccgacccgt gggcacgggc
     2581 cgaacccctt caatccgccc tctcctgccg ctggccgggc aaccctgccg ttggcgcgga
     2641 gacggccgct cttttccggt ggcgaagcct acgctggttt cggaaacttt gtaaaagccg
     2701 cgccggacgg gcggcagact ccgcggatga ggcttcgcct gatcgcagcg gccgccattc
     2761 ttgccgccgc cggctaagga ccgacgcgcg ggcggatgcg ccgtggcggc tggccggggt
     2821 cgcgcggcgt ctccaccagc tcccagcgcg gcagcggacg cgcggtcgcg ccaccttcgt
     2881 cggtgagtat ctcggccacg cggcgcacgg ccctgcgcag ctcgtcgcgc gccgccggcg
     2941 ttgcgccggc gaccaccttg gcgagccggc gcgcgatgat ctcctcggcg atgagggcaa
     3001 agccctccga atcgcggtcg cgtttgacga ccgactcggc gctgctgatg ccctcggcct
     3061 cgagcacggc aatcagccgg cgcacctccg ccgcttcccg ggcgggcagg cggtcgatcc
     3121 agctcatggg ggtcggcggc ttcctggatg cggccttctg ggtcgcgctg ctcttgccgc
     3181 ctcgccccgt cccggatttc gcgggcgctg gcgtgactcg gcgcgggacg cgcagcagcg
     3241 gcttgatttc gcgcgcgccc gggatcgcgg cccggacgtc ctcgagcgtg tcgaacgggc
     3301 cgtggatcag ctgctggcca aactcgttcg actgctcgtt gtcgaccagg aaccagcgct
     3361 ggtcggactt ctggactgcg aatcggccgt cgccggaggt gtaggcgcca gcgccagccc
     3421 ggacgaggtc atcgggtccg gctgggggct tctctttctt ggccatctgt ctccattgtg
     3481 gcgctcacgc gcccacgaag cgctcgaaca accgccaatg ccaggtctgg cgcacgacga
     3541 cgaaccacag cgcgaaggca cccaggacaa gcgcccactg gaccaggtcg agcggcgcca
     3601 gcgcaaacag gtcgcccagc ggccggatga gcgtcaggat cacgaataca aggcccagca
     3661 cgatcgccag cacggtcggc cggcgatcgg agctgcgctc ctgggtcacc gccagccagt
     3721 tggtcggcgg ctcgacgaac acgatcagtc ccagtccgac gaagaccagg aatgccgtca
     3781 gggccgattg cgcggccggc gtcgcgttga tgaccgccgc ctcctggagg gcggtgccag
     3841 ccgctgccgg caaggccgag acgctcagcc agatgctgcc gtagaacacg gccaacgcgg
     3901 cagcgctcga gagcagcgcg gccggcagca cgaaccggat caccatccgc gtcagcgatt
     3961 ggtgctgcac cttgcccggc tgcgcccaga gtgcgaggag cgcggccggg atgcccacgc
     4021 tgagcagcgt gatggccgac gcgttgcgca gcgcgatcgg gaagatgccc acgacgagcg
     4081 acgacacgat caccaggccc atcgtcgcga tgcgcgccag gaacaggctg atgatgtcga
     4141 acatgccgtt gacgatgcgc tggccctcct ccaccgcggg cgccagggcg gcgaacgagt
     4201 cttcggtaag gacgatgtcg gccacgcccc tgctcgcctg gctgccgctc gccatggcca
     4261 ccgcgagatt cgatttcttg agcgacagca cgtcgttcac gccgtcgccg atcatcgcca
     4321 cgtaggagcc gccggcgcgc aacgaggcca ccaggcgctc cttctgcgtg ggtgtgatcc
     4381 ggccgaagac gacgttgcgc cgcgcgacgg cggcgagctc ggtgtcgtcg agatcatcga
     4441 gctgcgtgcc cgagacgtgc ggcaggtcgc cggtaaggcc ggcctgccgg gcgagcgcca
     4501 cgaccgtgtc cgggtcatcg ccgctgatga tcttgacctg cacgccggcc tgacgaaagc
     4561 gctccaggat gggcccgacg cccgggcgca gctcgtcctc gagcaccacc agaccgagtg
     4621 gcgcgaaggt ggccgtgagg cgtgcgtcgt cgtcgtcgcc cgtgattgcg ttcgggtcgt
     4681 tgctcgtgag caggatgagc acccgcaggc cgctcgcggc gcgctcattg acctgcgctt
     4741 cgatgcccgg ccagcgcgcg gagtgcgcct cccccagcgc cggcttcaag agctgtggcg
     4801 cgcccagcac gtatgagttg ccgtcggtca tcgtggcccc gctccacttg cgcgctgacg
     4861 agaacggcac ctcgcgctgg accgacgcag cgtccccggg gagcgacgtc gcgatcgcct
     4921 cagcagtctt gtttcgctcc gtcgtgctgg cgacgagggt gcccagggag cggcggaggc
     4981 cggcctcatc gctggtaatc gggaccagct cacgcagcgc tagccgattg gcggtgagcg
     5041 tgcccgtctt gtccgtgcat aggatgtcga cccggctgag cgactcgacg gcgttggcct
     5101 gctggaccag cgcgccaaag cgcaccagcc gcacggcgcc cagcgcgtag gctacggcga
     5161 tcgagacaaa gagcccgttg gggatgagac ccgccagaac cgacgcctgg gcgatcgtct
     5221 cacccggcgc agcaacgtga atgatggcgg tcagcaggag cagcagctcc atgtacagca
     5281 cgagcacgag cgccagacgt atcacgctgt tgacgctgcg ctggagtggc gtcaggacgc
     5341 gccggaaggc cttggcgccc tgggtgatgc ggttcgcgag actgtcggta ccgacgcgct
     5401 cgaccacgta gcgaccactg cccgtcgcgc agaacgagcc ggagaagacc tcgtcgcccg
     5461 tccccttgcg gaccaggtcc gattcaccgg tgagaagcga ctcgtcggcc tggagcctgc
     5521 cgtcgacgag gcgtccatcg agcacgaact ggtcgcccgc ctggacgcgg atgaggtcgc
     5581 ccacaacgag ctcgcgcgga ttgactgttg tcgtcgtgcc atcgcgaatg acgctcgccg
     5641 tgggccgcgt gagcaggctg atgcggtcga gcgtccgctt ggcgcgcacc tcctgcacga
     5701 caccgaccac cacgttggtc ccgaccacca gcaccgacac cagcgcatcg agtgggcgcc
     5761 caacgatgac caggcccagg ccgaggagga acatgatgtt gttggtgaat gtgaacgtgt
     5821 tctcgacgaa gatctggagg taggtgcggc cgcccttctc ccgggcgtcg ttgcccaggc
     5881 ccctctgtcg gcgcgccacc acctctgcgg cgctgagccc ctctcttgcc acgctttgca
     5941 tgcgcccgaa aggttacccg gccgagcgca cccacctgac gaggttggcg gccagtttga
     6001 taagtgcgta cagggcgagg ccgacgagcg tgcctgtgac gttgacgatg acgtcgtcga
     6061 tgtccgcccg gcggtagcca tagcccaggt tggtcgatag cgcgtactgc gtcagctcaa
     6121 tcgtcgcgga gagcgcggta ccggccacga ggacgagcag ccacgggatc cgcctgacgg
     6181 ccagcggcac caggagaccg aagggcacga aggccaggac gttgccaatc aggatggcga
     6241 aggcaaacgt gccgggtccg tggcgcagcg ccggcaggat cgtcgcgagt ggcctgagat
     6301 tgacgcgcac gttcgattcg acgtctgcac ccaccggccg caacgtcagc acggcgagca
     6361 cgaccaggta ggcgaggagc agcgcgagcg cgagcctatc gatcctcaac caacgcctcc
     6421 ccgcacgggt ccggcgcatc gttctcaacg ttgagtacga gaagatccat tacgagcggg
     6481 acagcgctga cccggggcac gccgaagaac gaccacttca cggcgatgtc gtcgagcccc
     6541 gtgccactac cgggcgggta aggcagcgtg ccgtccaaca tgcacggacc cgtgcggaaa
     6601 tggacccaga ccgccaacac gtctcccggc tcaacatcga ctggatggaa gggctctgag
     6661 tcggagagcg cggccatgtt cgcggtctcg ctcacgccgt gcgcaagccg aaattccacc
     6721 gcatatgccc ccagataagc gggatctgtg ttcgtctcga ggccgttgac cgagatcggt
     6781 aggaggcccg agttcttcat ggtcgtcatc acgtagtagt cggtgctggg cgcatacgcg
     6841 aagaagtccc ccgatggatc gaggtgcgcc tgatccaccg cgaagtcgtt acccggcacc
     6901 agtggattca cgacgaacgc gacgaaagcc gcgccgacga gagctaccgc gagcgcgaga
     6961 gtcagcgcac gtttcatgtc gccacgaaag acgatgccac tacccgacat gtttcaggat
     7021 cgaagccatt gcgcgcgagc agcgcatacg ccttctggcg gcgtttgcgc tcgtcgggct
     7081 cacgcaagag tgcagcgcgc ttgcgctcga gcagcgcggt cgcggcggca aggttggggt
     7141 cgtctccatc agccgcatcc agccgctccc ccaagacctc gtcgacgacc tgacggccca
     7201 cgcccttctg ggccagctcg cgccgcagga cactctcccc gcgcggcctt gcccgatcgc
     7261 gactctctac ccacgttcgc gcaaacgact cgtcatcgag gtaaccgacc tccgcgaatc
     7321 gctcgataac ctggccgatc agcccctgcg ggtagcccag ctgttgcagc cgccgcctcg
     7381 tctcatcgac cgaccgcggc cgcaccgcca cgaaagccgc cgccgcctca atcaccggcc
     7441 caacctcggt gagctctccc ctcgcctcgc gccgcgcctt gaccgactcc ggcgtgctgc
     7501 gccttcgaat caccggacga tggtgcgctc accgcaccca tcgtccgccg aagtccccgg
     7561 gcggaggccc agatgctcgg aatcgagcgg acgcggcact cgagcacgct caactgctgg
     7621 acttgccgat ccgaacatgg cgattggaag gccaattgcc tcgttcaccg gacgttgggt
     7681 gcgttcatcg caccggagcg aatggcgagc gagcccttgg ggctggcatg ggtgagtcag
     7741 ctctaggacc cggccagatg gtccgatggc aacgagacca gcgggccggt caccgggtcc
     7801 tactctgcct ccgagattcc ttccacgggc acggtaatag tcgcgacctt ggcgcggatt
     7861 tcggtctcca acttctgcgc cacgtcctgg ttggccttca ggaattcctt ggcctgctcg
     7921 cgtccctggc ccaggcgcag gtcaccgtag ttgaaccacg ccccggtctt gctcacggtg
     7981 tccgacgcga caccgacgtc gagcagcccg ccctcgcggc tgatgccctc gccgtacatg
     8041 acgtcgaact cggcgacccg gaatggtgcc gcgaccttgt tcttgacgac cttcacgcgg
     8101 acgcgtgacc cgatggaatc agtgccgctc ttgatggtct cgatgcgccg gatgtcgagc
     8161 cggacgctgg cgtagaactt cagcgcgcgg ccaccgggcg tggtttccgg gttgccgaac
     8221 atgacgccga tcttctcacg cagctggttg gtgaagacga gcgcagtgtt cgagcgcgaa
     8281 acagcgccgg tcagcttgcg caacgcctgg ctcatcagcc tggcctggat acccatgaat
     8341 gagtcaccca tctcgccctc gatctcggcg cgcggcacaa gggccgccac tgagtcgacg
     8401 acgacgcagt cgacgccacc ggaacggatg agcgtctccg tgatctcgag cgcctgctcc
     8461 cccgtgtccg gctgcgacac cagcagctca tctacgttca cgccacaggc gcgcgcgtag
     8521 cccgggtcaa gtgcgtgttc cacgtcgatg aaggccacga ccccaccgcg cttctgagcc
     8581 tcagcgagga cgtgctggca gagcgtcgtc ttacccgatg actcggggcc aaagatctcc
     8641 gtgatacggc cgcgcgggat gccgcccacg cccaccgcca ggtccagcgc gatagagcca
     8701 gttgggatgg catcgaccgc ctgccgctcg gcagtaccga gcttcatgat cgagccacgc
     8761 ccgaactgct tctcgatcgc caggatggcc gcgtcgacgg cacgcgtgcg ctcgctgggc
     8821 ttctccgcct tctctacgcc gttgctctcc gccgttgact tcgtccccgt catcgccatc
     8881 ccagaaatcc ctcctgactg atcacgggga ccgagcgagt tatccctcgc cggactccgg
     8941 ctcgtcccag cgcggcttgc gcttgggctt gattacctcg actgagggcg ggggcgcgtc
     9001 cgacagcgat tgcagctgcg gctttgcgtc cttcgccttt ggcttcttct tggcttcctt
     9061 gtgagggcga tcccttgatc ccatttctca cgcctccacg gacccggtgc cacgttagcc
     9121 cgggccgctt gtctacgagt tcactccgac tttgcgccag cttgtctgtc acgcttgctc
     9181 atcccagatg gatggcggcg gttcaggcac tgccagctca gccctaagtt tagggaactt
     9241 gccctgcgcg agagcctgcc ggatgcgggc catgaagtcg gccaggtagg tcaggttgtg
     9301 gagcgtcgcc agccggtaga agagcagctc ctcagcgcgc agcaggtgcg ccaggtatgc
     9361 ccgcgagaac gaccggcagg cgcgacagcg gcagccctcc tggagcggcg ccggatcatc
     9421 gaggaagcgc gagttgcgca ggttgagccg cccggtcggc gtccagaccg tgccgttgcg
     9481 cgcgatgcgc gacggcagta ccgagtcgaa catgtcgatc ccggcatcga ccgcgtccag
     9541 caggtcggcc ggcgaaccca ggcccatcag gtagcgcggc cgcgggtcgt ccgcgagcaa
     9601 aggcacgacc acgtcgagcg tctcgcgccg ctgggcggcg ctctcgtcgc ccgccaggcc
     9661 gccgatgcac aggccgtcga aggccatgcc cgcgatgacc tgcgtcgact ctgctcgaag
     9721 gtcgggctcg aggccgccct ggatgatgcc aaacagcgcc tgatcggggc gcttgtgcga
     9781 gctcaggcag cgctcggccc acgagtgagt gcggaccatt gcctctgcca cctcgcgccg
     9841 gctcgatgcg tgaggcggga ccggctggtc gaggaccacc gcgatgtcgg acccgagcgc
     9901 ctcctggacg gcgatcgagt gctcgggcga gaacttgtgg atggagccgt cgaggtgcga
     9961 gcggaacgtg accccgtcgt catcgacgct gcggaagtcg cccaggctga ccacctggta
    10021 accgcccgag tcggtcagga tgggcctctg ccagctcatg aacttgtgca gcccgcccag
    10081 tcgggcgatg cgctcgtggc ccggtcgcag gtacaggtga taggtgttgc acaggatgat
    10141 ctgggcgccg atctcatcga ggtcgtccgg atcgagcgcc ttgacggtgg cgttcgtgcc
    10201 gaccggcatg aagacgggcg tctggatctc gccgtgcggg gtcgtgatgt gacccagccg
    10261 ggcgcgagtc gaaccgtctg ctggtgacgg cagcaggaca tcgaaggaca tcggccgact
    10321 gaccgggttg gtgtcgagct cgaccgtcat cagatgatct cctggcggtt gagcgagacg
    10381 accgccacgg caaccgcggc gaacaggtag atcagcagcg ccgggaacgc ctgctcgaac
    10441 ggcacgggtc gcaggaagat gccctggata ccgccggcag cgatgttccc cgcaaccccc
    10501 ggtgcccagc taaggacctg gttgccaacg gtcatcggca ggtactggaa ccactccggc
    10561 ccgatcagct ggaggtcgcc ctcgccaatg ccgccgccgt tgccaccgaa gaagcgtgcc
    10621 gcggcactga aggtcaggta cgtcgtgacg atgccctcgc ccaggtagag aacgatcccg
    10681 accaccgcgc cggcgagctg gctgcgcagc acgacggcaa cggcaaagcc cagcgcagcg
    10741 cgttcgagca cgacgacgaa cccaagcgcg aaattggtca cgaggtccag gagcccgttg
    10801 cccctgagcg ggctggcaat cgggatgccc tggctcaacg cggagacgaa cgtcatcgcc
    10861 agcgcgaaga agaagatgat cgcgcagccg accaacagca ctatcgacag tccggccgcc
    10921 ttggccagca ggtagcgggc gcgactctcg ccgcgcgcga tgacgttgcg caggacgccc
    10981 caattccagt ccgcgcccac gaaagccgcc gcatagacga tggccaggag accgcccagc
    11041 gttgtgaacg cgaattggtc gatcagcgca taggccgccg ggaactcgac gatgcacgat
    11101 tggccgaaat caccaatggt gatgtcgcag cccacgccac caattgatcg gccgatcaag
    11161 aagtagatca gtgcagtcag cgccagggcg acgaccagca ggacccaggt cgcggtgcgc
    11221 cgccgcgcct tcagcagctc ggcacgaatg agcctcatgc cgcgcccatc gatggcggcc
    11281 ccggatcagc tggggacagg gcggacaggg cgggcgcggc gtcggggcct gtgctggcgg
    11341 gcggcgcggc gacgttaccg gtgcgaccgg tgagctccag gaagatgctc tccaggtcgc
    11401 tccccggctc aagcccggag gcgtagacgc cggccgcggc caggacgcga ttgagctctg
    11461 cggcacgagc cggcgcaatg ccgaccgtga accacccggc accctcgccc gagtcgatgc
    11521 cgtacagcgg ccgatcggga gcgagtccgc gtagcaccgc ggcagccttg gtcatgtcct
    11581 ccggggcgac acgcacacgc actcgcccgg ccgactcgag cagctccgcg agcggtccct
    11641 cgcggatgag ccggccgtga tcgatgatgc ccacgacgtc ggcgagcatc tcgacttcgt
    11701 ggagcaggtg gctcgagatg atcaccgtct tcccgagctc ggcggtgaaa tagcgcagcg
    11761 tctcacgcat cgcgaccatg ccgcccgggt cgagaccgtt ggccggctca tcgagcagca
    11821 gcaggtcggg gtcggacagc atcgccgcgg cgatgcccag gcgctgtttc atgcccagcg
    11881 aataggtctg gacgggcgat ttcgcgcggc cgctcatgcc gacgtattcg agaacccggt
    11941 cgatccgggc tgggtccgtt ggcggacccg acgcgcccag gacgagcagg ttctccgcgc
    12001 ccgacaggta cgggtagaaa gacggcgtct caatgagcga gccaacccgg aatagccggg
    12061 tccggtcgtg ccacgtgtac ggcgtcccga agaggctgat cgtgcccgcg ttgggcctga
    12121 tcaggccggt gagcagccgc agcgccgtcg tcttgcctga gccgttcggc cccaggaagc
    12181 cgtagaccct gcccgtcggg acggacatgt cgagcccggt gagcgccacc tggcgaccgt
    12241 aggccttggt gaggccgcgc gtcgctaccg ccaggtccgg tgtcggcgca tcgctgcgcg
    12301 gcaggaactc cggacgcggc gcgggctgaa tgtcgcccgt catgcctcga cctcagccct
    12361 gccctcgtgg gtgatgccgt cccacagccc cgagtcagca cggtcggcct tgcgcggcgc
    12421 gggtacctcg tagccaagcc cgcgcagatg gtcgcgtgcc cgctcggtgg cgacgcggcc
    12481 ctgcggcgtg cggtcgagga agccaagctg cagcagatat ggctcgtaca cgtcctcgat
    12541 ggtctccacc tcttcgccga tgaccgcggc cacggccgcg cccccgacgg gaccggacgc
    12601 gaacttctgg acgatcgcag ccaacaggcg gcggtcggtc gtgtccagtc cctcgtcgtc
    12661 gatatccatc gcgttcatcg cggccgtcgc ctccgcggcg ccgatgtggc ccgatgagcg
    12721 cacctgggca tagtcgcgca cgcggcgcag caatctgttg actacgcgcg gcgtgcctcg
    12781 tccgcggcgc gcgatcacct ggatggcgtc cggttcgatg tccacgcgga ggatgccggc
    12841 cgagcgctgg acgatcgttg cgagctcgtc ctgcgagtag tagtcgagcc gatataccgc
    12901 cccaaagcgg tcgcgcaacg ggccgccgat ccgccccgcg cgggtcgtcg cgccgaccac
    12961 ggtgaacggc ttgatgctga gccgcagcga ccgcgccgat gggccgcgac cgatcatgac
    13021 gtcgatcgcg aaatcctcca tcgccgggta caggatctcc tcgaccgcgt ggttaaggcg
    13081 atggatctcg tcgatgaaca gcacgtcgcg ctcatcgaga ttggtcagga tcgcggccag
    13141 atcgccgggc cgctcgatgg ccggtccgga cgtggcatgc aggttcaccc ccaactcgcg
    13201 ggcgatgatg ttggccagcg tggtcttgcc caggccgggc ggtccgtaca gcaggacgtg
    13261 gtcggccgcc tcttcgcgct ggcgggcagc tgcgagcagt atctcgaggt tctgcttgac
    13321 ggtcccctga ccgatgtact cggccagcag gcgtggtcgg agagacgtct cgacggcgac
    13381 ttcttcgacc gacagggtgc ccgtgacgac ggggcggtcg ggctccaggc cgggttcctg
    13441 catcgggcgt cagtctacca gtttttgacg ggtatagaac gcatgttcga cgctccgtgg
    13501 tccagttccc agcagggtca caccctcttg tggatcatgc gctcgatcgc ctccgcggcg
    13561 tcctcgcccg cgtcgatggc gttttcgagc gccagataca gctcgcgcca cttgatgacc
    13621 tcgagcgcgt ctgttgagtc gcggaacagc ttgccgatgg cggcccgcga tacgccgtcc
    13681 gcctcatgct cgagctcatg gaccttggcc aggtgttcgc cgatgtcttt catcttgttc
    13741 agcttgccca gcgccttggc gagctcggcg ccctgtaggg ccaggatctg ggccagccgg
    13801 cggcagtcct ccgtgggtgc cttgacgtcg tagatcgaaa ggctctcgcc gacggcctgg
    13861 atcaagtcga caacgtcatc caaccggacc gtcagctcat ggatgtcctc gcggtcgaac
    13921 ggggccacga agg
//
LOCUS       DATBRX010000193         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2310574, whole genome shotgun sequence.
ACCESSION   DATBRX010000193 DATBRX010000000
VERSION     DATBRX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310574"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VIF08_07525"
     CDS             complement(<1..954)
                     /locus_tag="VIF08_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8135877.1"
                     /translation="MTAEEMVALSKQYSLYEWSAQSAVDPIPVERAEGVYFYSPDGKR
                     YLDFNSQLMSVNIGHGDKRVVDAIAEQASKLAYANPFMAHEPRARLGQKLAEIAPGDI
                     DVFFFTNGGAEANENAIKLARQATGRHKVLARYRSYHGATGQVITATGDPRRWAAETG
                     VWGVVRIPDFQPWGDPAPRDVASVLRETEQVIVYEGPQTIAAMIIEPVVGTNGILIPP
                     DGLMQGLREMCTRYGIWLIADEVMSGFGRTGRWWAVDHWDVVPDLITMAKGLTASYLP
                     LGAVGMSRSVAEKFKDKVFYGGLTYNSHPMGCAAALATIGVY"
     gene            complement(1115..1351)
                     /locus_tag="VIF08_07530"
     CDS             complement(1115..1351)
                     /locus_tag="VIF08_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HEY8135878.1"
                     /translation="MKRILLILGAALTLATAAACGPGAGTTSSFPVNTTGPTLAPTSA
                     PSDMPSDMPSEMPSEMPSEMPSEMPSDEASPEAS"
     gene            1520..1837
                     /locus_tag="VIF08_07535"
     CDS             1520..1837
                     /locus_tag="VIF08_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135879.1"
                     /translation="MPLHRLRGLPTAQAEGRSVYEGCGAGVPEAAGLPDAGATSDADG
                     MAEPGGTADPDGAGTTEGVGVGGTYVHAGPVGCAQPPRSRAPVRPSGTTHSAIRLRIR
                     SIC"
     gene            2014..3225
                     /locus_tag="VIF08_07540"
     CDS             2014..3225
                     /locus_tag="VIF08_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY8135880.1"
                     /translation="MSQNRRAPKFVALFLAVPVVGLVYVAAFFGGLARVFRPALTPVV
                     GASIIGTTFAAQALRAEPAKRPAPAVVAIALAIALVAPAVSNVPVAVAADPQDAVVRL
                     TMDTVGSPYVFGAEGPNKFDCSGLVYWVFKEAGELPRIGGSRMGARSYMRWFVARGRW
                     SKDESDARPGDLVVWGDGHHIGVYIGPNRAVSALNPKLGVRVHDLKIPHAPTQFLLIN
                     WGPNDGGGGDGGGGDGGGGDNGGGDNGGGDNGGGNDATPTPDDGPSTGNGASNGDTGF
                     DPPAATDAPTPKPTPKPTPKPTDAPPDSGGGQGVASGQAVTESNVGVLPPATRTNPQP
                     SGSNGVAIATVNLRELPDATSRILGWVGSSGYVKIVGTAFSPQGNLYYRVTTASGASG
                     WVYSHWVLRTQ"
     gene            complement(3237..>3732)
                     /locus_tag="VIF08_07545"
     CDS             complement(3237..>3732)
                     /locus_tag="VIF08_07545"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135881.1"
                     /translation="VGLHAAMFNAAHHFDDGRWGSPLRLLWMVFRYRPGRMEIELDGS
                     TSVQTRGLMAVVANGPYAGAGMTMAPDARLDDGLFDVRVFRHFSKLELLRHLASIMFG
                     RRAYVPRVSTYRAARVRVTGRRPLPCRADSLDLGVTPMECRVLTRSLKVVVGPEFGAA
                     PGAR"
BASE COUNT          597 a         1291 c         1292 g          552 t
ORIGIN      
        1 gtagaccccg atcgtcgcca gcgccgcggc gcagcccatc gggtgcgagt tgtacgtcag
       61 gccgccgtag aacaccttgt ccttgaactt ctcggccacc gagcggctca tgcccaccgc
      121 gccgagcggc aggtacgacg cggtgaggcc cttggccatc gtgatcaggt cgggcaccac
      181 atcccagtgg tccacggccc accagcgccc ggtccggccg aagccgctca tgacctcgtc
      241 ggcgatgagc cagatgccgt agcgcgtgca catctcgcgc aggccctgca tcaggccgtc
      301 gggcgggatg aggatgccgt tggtcccgac cacgggctcg atgatcatcg cggcgatggt
      361 ctgcgggccc tcgtagacga tgacctgctc cgtctcacga aggaccgaag cgacgtcgcg
      421 cggcgcgggg tcgccccacg gctggaagtc ggggatgcgt acgacgcccc agacgcctgt
      481 ctccgccgcc cagcggcgcg ggtcgcccgt ggcagtgatg acctggccgg ttgcgccgtg
      541 ataggagcgg tagcgcgcca gaactttgtg gcgaccggtc gcctggcgcg ccagcttgat
      601 ggcgttctcg ttcgcctccg cgccgccgtt ggtgaagaag aagacgtcga tgtcgcccgg
      661 cgcgatctca gcgagcttct gaccgaggcg ggcgcgcggc tcgtgggcca tgaagggatt
      721 ggcgtaggcc agcttcgacg cctgctcggc gatggcatcg acgacgcgct tgtcgccatg
      781 gccgatgttg acggacatca gctgcgagtt gaagtcgagg tagcgcttgc cgtcgggcga
      841 gtagaagtag accccctccg cacgctcgac cgggattgga tcgaccgcgc tctgggccga
      901 ccactcgtac agcgagtact gcttggagag ggcgaccatc tcctcggcag tcatggagct
      961 aggcgttgag atagccatgc gacggggcct cctcttgggc cccgaagtct agccgcacga
     1021 acgccccggg cacgcgcgtc agcccttggg cgcgcgacga cagcccgggc aatttgtcgc
     1081 ccgggctgcc gccggaggag agggagggag cgttctagga agcttcgggc gatgcctcgt
     1141 cgctgggcat ctcgctgggc atctcgctcg gcatctcgct cggcatctcg ctgggcatgt
     1201 cgctgggcat gtcgctcggc gccgatgtcg gcgcaagcgt cggcccagtc gtgtttacgg
     1261 ggaacgatga cgtggtgccg gcgcccgggc cgcaggctgc ggccgtcgcg agcgtcagag
     1321 cagcgccaag gatcagcaga attctcttca aagcacagtc ctcctaggta cctcaatcaa
     1381 agaagcgccc cggaaatggc cgtgccgtga ccgaccgccc tgtcccacgc aagcgcggta
     1441 agcacctctt gtagtcagac tcgatcttcc ccgctgcccg gttgcaggcg gtcgcccggt
     1501 ttgggtcggc tctctaacgg tgccgttaca ccgactgaga gggctgccga cggcccaagc
     1561 ggagggccgc agcgtctacg aaggctgcgg cgccggagtc ccggaagcgg ccggactgcc
     1621 cgacgcgggc gccacttcgg atgccgacgg gatggctgag cccggcggca cggccgatcc
     1681 ggacggagcg ggcacgaccg agggcgtcgg cgtgggcggc acgtacgtcc acgcgggacc
     1741 cgtgggctgc gcgcagccgc ccaggagcag ggcgccggtg aggcccagcg gcacgaccca
     1801 cagtgcaatc cgtctgcgaa tccgcagcat ctgctgattc ttgccgcacg agtcgccaaa
     1861 atgggtgtgc aatttgcgtt acagatgggc atactgcacc caacggcccc tgagggggca
     1921 cccgctacca ccgagaaaag gcaagtcaat accgccccag cggatgacga ggccaaggcc
     1981 gtgtctggat tgcactaggg aggcaatctc gaggtgtcgc agaaccgtcg cgcgcccaag
     2041 ttcgtcgcgc tctttctcgc cgtgcccgtc gtgggccttg tctacgtggc cgcgttcttt
     2101 ggcggtcttg cgagggtgtt ccgccccgcg ctgacgcccg tcgtcggcgc ctcgatcatc
     2161 ggcacgacct tcgccgcaca ggcgcttcgg gccgaaccag ccaagcgtcc cgcgccggcg
     2221 gtcgttgcca tcgcgctggc cattgccctc gtcgcgcccg ccgtcagcaa cgtgccggta
     2281 gcggtcgccg cggacccgca ggatgccgtc gtccggctca cgatggatac cgtcggctcg
     2341 ccctacgtct tcggcgccga aggccccaat aagttcgact gctctggtct cgtctactgg
     2401 gttttcaagg aggccggcga actgccgcgc atcggtggct cgcgcatggg cgcgcgctcg
     2461 tacatgcgct ggttcgtggc tcgcggccgc tggtccaagg atgaatcgga tgcgcgaccg
     2521 ggcgacctgg tcgtgtgggg cgacgggcac cacatcggcg tctacatcgg ccccaaccgc
     2581 gccgtcagcg cgctcaatcc caagctgggc gtgcgcgtcc acgatctcaa gatcccccac
     2641 gccccgaccc agttcctgct gatcaactgg ggcccgaacg atggcggcgg cggcgatggc
     2701 ggcggcggcg atggcggcgg cggcgacaac ggtggtgggg ataacggcgg cggcgacaac
     2761 ggcggcggca atgacgcgac gcccacgccc gatgacggcc cctcgaccgg caacggcgcg
     2821 agcaacggcg acacgggctt cgatccgccg gccgcgaccg acgcgcccac acccaagccg
     2881 acgcccaagc cgacacccaa gcccacggat gcgccgcccg actccggtgg cggccagggc
     2941 gtggcgtccg gccaggccgt cacggagagc aatgtcggcg tgctacctcc agcgacgcgg
     3001 accaacccac agccgagcgg ctccaacggc gtggcaatcg ccaccgtcaa cctgcgcgag
     3061 ctgcccgacg cgacgtcgcg catcctcggc tgggtcggca gcagcggcta cgtcaagatc
     3121 gtgggtacgg cgttcagccc gcagggcaac ctgtactaca gggtgaccac ggcgagcggc
     3181 gcgtcaggct gggtctactc gcactgggtg ctgcgcacgc agtaaccggc cgggatctag
     3241 cgcgcgccgg gggcggcgcc gaattcaggg cccacgacga ccttcagcga ccgcgtgagc
     3301 acgcggcatt ccatgggcgt cacgcccagg tccagcgaat ccgcgcggca gggcagcggt
     3361 ctgcgcccgg tgacgcgcac ccgggcagcg cgataggtgc tcacgcgcgg cacgtacgcg
     3421 cgtcggccga acatgatcga cgccaggtgg cgcagcagct ccaacttcga gaagtggcgg
     3481 aaaacgcgca cgtcgaacag gccgtcgtcg agccgcgcat cgggcgccat cgtcatgccg
     3541 gcacccgcat aagggccatt cgcgaccacc gccatcaggc cgcgcgtctg gacgctggtt
     3601 gagccgtcga gctcgatctc catgcgtccg ggtcggtagc ggaacaccat ccacagcagc
     3661 cgcagcggcg aaccccagcg gccgtcgtcg aaatggtgcg ccgcgttgaa catggccgcg
     3721 tgcaggccga cc
//
LOCUS       DATBRX010000194         7967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3764235, whole genome shotgun sequence.
ACCESSION   DATBRX010000194 DATBRX010000000
VERSION     DATBRX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3764235"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4218)
                     /locus_tag="VIF08_07550"
     CDS             complement(<1..4218)
                     /locus_tag="VIF08_07550"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8135882.1"
                     /translation="MTRRVATRPPAAPATDPLAAFSAATRDWFGASFDAPTAAQAQGW
                     PAIATGAHTLICAPTGSGKTLAAFLFALDRLLAEPVPEDPLRRLRVLYVSPLKALVHD
                     VNRNLRSPLAGISLAARNRGESGDREVRVAMRTGDTPADERREFGKRPPDILVTTPES
                     LYLLLTSQAREALRGVDWVIVDEIHALAATKRGAHLALSLERLEELCVKPPQRIGLSA
                     TQRPLSVVAGYLGGRQAATSDDKPGEPRPVTIVDAGVRKTLELQVVVPVEDMAAIGQV
                     LPPEQQKGGSAAAPDQRSSIWPQIHPKILELIRAHRSTIVFVNSRRLAERLALHLNEL
                     AGEELVRAHHGSLAREERLLIEEALKDGRLPALVATSSLELGIDMGAVDLVIQVESPG
                     SVARGLQRIGRAGHQVGEPSRGVIFPKYRGDLLEAAVVTQRMLAGEIEPTVIPRNPLD
                     VLAQQLVATAAERRWLADDLYNLARHAENFADLGRDSFDATLGMLAGHYPGDEFAELR
                     PRVVWDRAAGTVESRRDARTVAVISGGTIPDRGLFGVYIADEADGATTRAKSSRGGGG
                     RRVGELDEEMVYELRETEVILLGASAWRVDQITHDRVLVTPAPGEPGKVPFWKGDGPG
                     RPVELGRALGELTRTLGESALAGSRGRARAEKTLQDEHRLDALAAKNLLDYLAEEREV
                     AGALPTDRTIVIQRFRDDLGDWRICLLTPFGARVHAPWALAIEARLRERLGLEVQPIW
                     SDDGIVVRLPMTDIAGTDGIGLSGGLSDDAGESTVNPIVAAEEAIRIGADDVEELVIG
                     ALGGSSLFSSHFRENAARALLLPRRRAGQRTPLWQMRQRSAQLLGVASRYGSFPIVLE
                     TYRECLQDVFDLPSLKGILAAIERREINLVSVETRRASPFASSLMFDYIAQYMYEGDA
                     PLVDRRAQALALDRDLLRELLGAEELRELLDRDALAELELELAGLTPERAAGSADAVH
                     DLLRRLGDLTPAEVAARVRGQDERARERAAGEWLEALAADRRALKVRINSEPRWIAME
                     DLARYRDALGVMPPVGVPEAFLAPVADALNGLLLRWARHHGPFLATEPAARWGLPLAD
                     VEVELERLLVAGALLRGEFRPDGVEREWCHPDVLRLLRRRSLARLRREIEPVDSAALA
                     RFLPRWQGVGERRGGIDRLAEVVAQLEGMPLPASALERDILPSRVAGYSPRLLDELGA
                     AGEVVWLGLGSLGQDDGRVALYRPDRLALLLPPTESRAADAPSGWLVDAIRAHLRDRG
                     ASFYRDLIASALRAGAERGEPPVRERDLLDALWSLVWATEVTNDTFAPLRALRWPRKG
                     SSRPRHTNVARMGPPEAAGRWSLVSHQPASETERRHAQALRLLDAYGVVTRDSVAAES
                     LPGGFSAVYPVLRELEERGRVRRGYFVE"
     gene            4416..5846
                     /locus_tag="VIF08_07555"
     CDS             4416..5846
                     /locus_tag="VIF08_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8135883.1"
                     /translation="MLLLALLAPAGGAFAGERTAPYVVLFDTAAVSVPDDTLDASAAS
                     AASFRFVAAPQALRAATLATASPATTRGANTRVDASRVDTAIRAVAGRNHVRVNSVYA
                     NAVGGFAASLNATQLAAISANPAVAAVIPDLAVSIDDGSVGGEASVMRNVVRPAWQVP
                     AGVRRVGARTNAVRSFTQGSQHVDADVAILDTGVDRDHPDLNVVGGYNCTSSKRDSWD
                     DNNGHGTHVAGIVGALDNSFGVVGVAPGVRIWSVKVLDHKGNGFVSWLVCGIDWVTAQ
                     RDRGRARFEVANMSISFNVGPSPNSTCANGEDVIHQALCRSVAKGTTYAVAAGNNSHD
                     ARRNRPAAYDEVITVSAMADYDGKGGGLGKPSDSCPTRYEPDDSFTNFSNYGLDIDLI
                     APGRCVLSTYMGKRYAFMSGTSMATPHVAGAAALYRAMYPSAKPAQVRRALIAVGKLD
                     WRTGTDPDRNPEPALWVGGFLRPPKG"
     gene            complement(5843..6667)
                     /locus_tag="VIF08_07560"
     CDS             complement(5843..6667)
                     /locus_tag="VIF08_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8135884.1"
                     /translation="MTNWQGYIEVPGGRIYAEVEGDGPPVVFVHAGVAHLRMWDEQAA
                     ALRDAYRVIRYDTRGYGKTRVDDVPYSNSGDLGLVMDHFGADSAHVVGLSRGAQIALD
                     FAVNDPARTRSLTWVAGGVRGLEVPDDPRDEAVWHQTEALEKTKDWEKIVELETQLWT
                     DGPDQPPTRVAPDVRSRMIQWNLESYRADQSPSQVTAPEFAAADHLDRLTMPVLAMWG
                     TLDVTSTVKSGEWLAANLPGIRSRVFDGVAHMVNLERPAEFSQILREFLDQAEAGR"
     gene            complement(6664..7818)
                     /locus_tag="VIF08_07565"
     CDS             complement(6664..7818)
                     /locus_tag="VIF08_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEY8135885.1"
                     /translation="MTGSLLVYGDAALTYDFGPEHPLTPRRFPPSIDLIRTLGATNIV
                     EPRVATDDEIARLHTRDYINTVRRFGADPWHHMPAAGIGPGDCPPFEDMHEASARVAG
                     GSLEGVDRILGGDVDHAFNPAGGLHHAMAGRASGFCIYNDVALAAARARDAGHRVMYV
                     DLDVHHGDGTQALFWDDPNVLTVSVHETGRTLFPGSGFVDERGGPEALGTKVNLPLHP
                     LTGDGSWLAAIERVLPALAEGFRPTFLVTQHGCDTHVYDPLAHLRLTTRAYRQATLLL
                     DGLAHQYAEGRWFATGGGGYDAYRVVPRSWALVWLAQAHLEPPIETAADWRERWAAEA
                     DRYSQGPPPTLMLDPADLVKPESDDLIEDNAKTVERALSGALELIGRDKP"
BASE COUNT         1207 a         2862 c         2763 g         1135 t
ORIGIN      
        1 ctcgacgaag tagccgcgtc ggacgcggcc gcgctcctcc aactcgcgca ggaccgggta
       61 gacagcagag aagccgccgg gcaggctctc cgccgcgacc gaatcgcgcg tcaccacgcc
      121 atatgcgtcg agcaggcgca atgcctgggc gtggcgccgc tccgtttcag aagcgggctg
      181 atgcgatacg agcgaccagc gaccggccgc ctccggcgga cccatgcgcg ccacgttggt
      241 gtggcgcggc cgcgacgatc ccttgcgcgg ccagcgcagg gcgcgcagcg gcgcaaaggt
      301 gtcgttggtc acctccgttg cccagaccag cgaccacagc gcgtcgagca ggtcgcgctc
      361 gcggaccggc ggctcacctc gctccgcacc ggctcgcaat gcggacgcga tcaggtcgcg
      421 gtagaaggag gcgccgcggt cgcgcaggtg ggcgcggata gcgtcgacca gccagccgct
      481 gggggcgtct gccgccctgc tttcagtcgg cggcagcagc agcgccagtc ggtcgggacg
      541 gtagagcgcc acccggccgt catcctggcc gagcgaccca aggcccagcc acacgacctc
      601 gcccgcggca cccagctcgt cgagcaggcg cggcgagtag ccggcgacgc gcgacggcag
      661 gatgtcgcgc tccagcgccg atgccggcaa cggcatgccc tccagctgcg cgacgacttc
      721 ggccaggcga tcgatcccgc cgcgtcgctc gcccacgccc tgccaacgcg gtaggaagcg
      781 cgccagggcc gcactgtcga ccggctcgat ctcgcgccgc agccgggcca gcgagcggcg
      841 gcgcagcagg cgcaggacat cagggtgaca ccactcgcgc tcgacgccgt cgggccggaa
      901 ctcgcctcgt agcagggcgc cggcgacgag cagccgctcg agctccacct cgacatcagc
      961 cagtggcagt ccccagcgcg cggcgggttc agtggccagg aatggtccgt ggtggcgcgc
     1021 ccagcgcagc agcaggccgt tcaacgcgtc tgcgaccggc gcgaggaatg cctccggcac
     1081 gccgaccggc ggcatcacgc ccagcgcgtc tcggtagcgc gccaggtcct ccatggcgat
     1141 ccagcgcggc tcgctgttga tgcgcacctt cagggcgcgc cggtcggcgg cgagcgcctc
     1201 cagccactcc cctgcggcgc gttcgcgggc gcgctcgtcc tggccgcgca cccgggccgc
     1261 gacctcggcc ggcgtgagat cgcccagccg gcgcagcaga tcgtgcaccg catcggccga
     1321 cccggcagct cgttccggcg tcagccccgc cagctcaagc tccagctccg cgagcgcgtc
     1381 gcgatccagc agctcgcgca gctcctccgc gccgagcagc tcgcgcagca ggtcgcggtc
     1441 cagggcgagc gcctgggcgc gccggtcgac gagtggcgcg tcgccctcgt acatgtattg
     1501 cgcgatgtag tcgaacatga gcgagctcgc gaacggcgag gcgcgcctcg tctcgacgct
     1561 cacgaggttg atctcgcgcc gctcgatcgc ggccaggatg cccttgagcg acggcaggtc
     1621 gaacacatcc tggaggcatt cgcggtaggt ctcgagcacg atcgggaagc tgccgtagcg
     1681 gctggccacg cccaatagct gggccgaacg ctggcgcatc tgccataacg gcgtgcgctg
     1741 gccggcgcgg cggcgcggca gcagtagtgc ccgcgcggcg ttctcgcgga agtggctcga
     1801 gaagagcgac gagccgccca gggcaccgat gaccagctct tccacgtcat cggcgccgat
     1861 ccgaatcgcc tcctccgccg ccacgatcgg attcactgtc gactcacccg cgtcgtcgga
     1921 gaggccgccg ctcaggccga tcccgtccgt gcccgcgatg tcggtcatcg gcaagcgcac
     1981 gacgatgccg tcgtcgctcc agatgggctg cacctccaat ccgagccgct cacgcaggcg
     2041 ggcctcgatt gccagcgccc acggcgcgtg aacgcgcgcg ccgaacggcg tgaggaggca
     2101 gatgcgccag tcacccaggt cgtcgcggaa gcgctggatg acgatggtcc gatcggtcgg
     2161 cagggcgccc gcgacctcgc gctcctcggc caggtagtcg agcaggttct tcgcggcaag
     2221 cgcgtcaagg cggtgctcgt cctgcagcgt cttctccgcg cgcgcccggc cgcgcgagcc
     2281 tgccagcgcc gattcgccaa gggtgcgcgt cagctcgccc aacgcccggc ccagctcgac
     2341 tggccgcccc gggccgtcgc ccttccagaa aggcactttg cccggctcgc ccggcgcggg
     2401 cgtcaccagg acacggtcgt gggtgatctg gtcgacgcgc caggcgctgg cgccgagcag
     2461 gatcacctct gtctcgcgta actcgtagac catctcctca tcgagctcgc cgacacgacg
     2521 gccgccgccg ccccgtgacg acttcgcacg cgtcgtcgcg ccatcagcct cgtccgcgat
     2581 gtacacgccg aacaggccac gatcggggat cgtgccgcca ctgatgaccg cgaccgtgcg
     2641 cgcgtcgcgt cttgactcaa cggtgccggc ggcgcggtcc cagacgacgc gcggccgcag
     2701 ctcggcgaac tcgtcacccg ggtagtgacc ggcgagcatg cccagcgtgg catcgaacga
     2761 gtcgcggccc aggtcggcga agttctccgc gtggcgcgcc aggttgtaca ggtcatcggc
     2821 cagccagcgc cgctcggccg cggtcgcgac cagctgctgg gcgagcacat cgaggggatt
     2881 gcgcggaatg acggtcggtt cgatctcgcc cgccagcatg cgctgcgtca ccaccgccgc
     2941 ctcgagcagg tcgccgcggt acttggggaa gatcacgccc cgggaaggct cgccgacctg
     3001 gtgtccggca cgaccgatgc gctggagccc acgtgctacc gagcccggcg attcaacctg
     3061 gatgacgaga tcgaccgcgc ccatgtcgat gcccagctcg agcgaggacg tggccaccag
     3121 cgcgggcagt cggccgtcct tcaatgcctc ttcgatgagc agccgctcct cgcgcgcgag
     3181 cgagccatgg tgggcacgca ccagttcctc gccggcgagc tcgttgagat gcaaagccag
     3241 ccgctcggcg aggcggcgcg agttgacgaa cacgatcgtc gagcgatggg cccggatgag
     3301 ctcgaggatc ttcggatgga tctgcggcca gatggagctg cgctggtcgg gcgccgccgc
     3361 agacccgcct ttctgttgtt cgggaggcag cacctggccg atcgccgcca tgtcctcgac
     3421 cggcaccacg acctgcagct cgagcgtctt gcgcaccccg gcatcgacga tcgtcaccgg
     3481 ccgcggctca cctggcttgt catcgctcgt ggctgcctgt cggccaccca ggtagccggc
     3541 gacgacagac agcggccgct gggtcgcgga caggccgatg cgctgcggcg gcttgacgca
     3601 cagctcctcg agccgctcga gcgacagcgc caggtgagcg ccgcgcttgg tcgctgctag
     3661 agcgtggatc tcgtcgacga tcacccagtc gacgccgcgc aacgcctcgc gcgcttgcga
     3721 ggtcagcagc agatagagcg attcgggtgt cgtgaccagg atgtccggcg ggcgcttgcc
     3781 gaactcgcgc cgttcgtcgg ccggggtgtc gccggtgcgc atcgcgacgc gaacctcccg
     3841 gtcgccgctc tcgccgcgat tccgcgccgc gagggagatg cccgccagcg gggagcggag
     3901 gttgcggttc acgtcgtgga ccagcgcctt caagggggac acgtagagca cgcgtagccg
     3961 gcgcagcgga tcctccggca ccggctcggc caacaggcgg tccagcgcaa acaggaatgc
     4021 ggcgagcgtc ttgcctgagc cagtgggcgc gcagatgagc gtatgggcgc cagtggcgat
     4081 ggccggccag ccctgcgcct gggccgcggt gggcgcgtcg aatgaggcgc cgaaccagtc
     4141 gcgcgtggca gcgctgaacg cggccagcgg atcagtggcc ggtgccgcgg gtggcctggt
     4201 ggcgacgcgg cgggtcacgg gacggcgagt ataagttgac cgcccctata acccatcgtc
     4261 ctccccccaa ccggtaccac gtcgtcgttt agaaacggcg cgcgggtccg tacggtcagt
     4321 cggcgatggc aagaatggga gggggcaaat ctgccggaac acccgcgagg gcgttcgcca
     4381 ggtcgcgcct ccgcagacgc ttactcttcc tgacgttgct gctgctggcg ctgctcgccc
     4441 ccgcgggcgg ggcgtttgcc ggcgagcgga cggcgccgta cgtcgtgctc ttcgacacgg
     4501 cagccgtgag cgtccccgat gacaccctcg acgccagcgc cgcgtccgca gcatccttcc
     4561 gcttcgtcgc cgcgccgcag gcgctgcgcg ccgcgacgct ggccaccgcg tcacctgcga
     4621 cgacccgggg cgccaatacc cgggtagacg cgtcgcgcgt cgacaccgcg attcgcgctg
     4681 ttgccggccg caaccacgtc cgcgtcaatt ccgtttacgc caacgcggtg ggcggcttcg
     4741 cggccagcct caacgcgact cagctcgccg ccatctccgc caacccggcg gtggccgccg
     4801 tgatccctga tctcgcggtc agcatcgacg acggatccgt cggcggcgag gcgagcgtca
     4861 tgcgcaatgt cgtacgtccg gcgtggcagg tgcccgcggg cgtgcgccgt gttggtgccc
     4921 ggaccaacgc cgtccggagc ttcacccagg gcagccagca cgtcgacgcc gacgtggcca
     4981 tcctcgacac gggcgtcgac cgcgaccatc ccgacctcaa cgttgtcggc ggctacaact
     5041 gcacgtcctc caagcgcgac agctgggacg acaacaacgg ccacggcacg cacgtcgccg
     5101 gcatcgtcgg ggcgctcgac aacagcttcg gcgtggtcgg cgttgcgccg ggcgtgcgca
     5161 tctggtcggt caaggtgctc gaccacaagg gcaacggctt cgtgtcatgg ctggtgtgcg
     5221 gcatcgattg ggtcacagcg cagcgcgacc gcggccgggc ccggttcgaa gtcgccaaca
     5281 tgagcatcag cttcaacgtc ggcccctcac ccaacagcac gtgcgccaac ggcgaggacg
     5341 tcatccacca ggcgctgtgc cgctcagtgg cgaaggggac gacctacgcg gtcgctgcgg
     5401 gcaacaacag ccacgacgcg cggcgtaacc ggcccgcggc atacgacgaa gtcattacgg
     5461 tctcggcgat ggctgactac gacggcaagg gcggaggcct cggcaagcca tccgattcgt
     5521 gccctacgcg ctacgagccc gacgattcgt tcaccaactt ctcgaactac gggctcgaca
     5581 tcgacctgat cgcccccggc cgctgcgtgc tctcgaccta catgggcaag cgttacgcgt
     5641 tcatgagcgg cacatcaatg gccacgccgc acgtcgccgg cgcggccgcg ctctatcgcg
     5701 ccatgtaccc gagcgccaag cctgcccagg tccggcgggc gctcatcgcg gtgggcaagc
     5761 tcgactggcg aaccggcacc gatccggacc gcaatcccga acccgcactt tgggtgggcg
     5821 gcttcctccg gccgccgaag ggctagcgac cggcctcggc ctggtcgagg aactcgcgca
     5881 ggatctggct gaactccgcc ggtcgctcga ggttgaccat gtgggccacg ccatcgaaga
     5941 cgcggctgcg gatgcccggc aggttggccg ccagccactc accgctcttc accgttgacg
     6001 tgacgtcgag agtgccccac atcgcgagga ccggcatcgt caaccgatca aggtggtctg
     6061 cggccgcgaa ctcgggggcg gtgacctggc tggggctctg atcagcgcga tagctctcga
     6121 ggttccactg gatcatccga ctgcggacgt cgggagcgac gcgcgtcggc ggctggtcgg
     6181 ggccgtcagt ccagagctgc gtctcgagct cgacgatctt ctcccagtcc ttggtcttct
     6241 cgagcgcctc ggtctggtgc cacacggctt cgtcgcgcgg gtcatcggga acctccagcc
     6301 cgcgcactcc gccggcgacc caggtaagcg acctggtgcg cgccgggtcg ttgactgcaa
     6361 agtcgagcgc gatctgggcg ccgcgcgaca gaccgacgac gtgcgccgag tcggcgccga
     6421 agtggtccat gaccaggccc aggtcgcctg aattggagta cggcacgtca tcgacgcgcg
     6481 tcttgccgta gccgcgcgtg tcgtagcgga tcacccggta ggcgtcgcgc agcgccgccg
     6541 cctgctcatc ccacatgcgc aggtgcgcga cgccagcgtg gacgaaaacg actggcggcc
     6601 cgtcgccctc aacctccgca tagatcctgc cgccggggac ctcgatgtac ccctgccaat
     6661 tggtcacggc ttgtccctcc ctatcagctc aagtgcgccg ctcagcgctc gctcgacggt
     6721 cttggcgttg tcctcgatga gatcgtccga ctccggcttg accaggtcgg ccgggtccag
     6781 catgagcgtc ggcggcggtc cctggctgta tcggtcggcc tcggcggccc aacgctcgcg
     6841 ccagtccgca gccgtttcga tgggtggctc caggtgggcc tgggccagcc atacgagcgc
     6901 ccacgagcgg ggcacgactc gataggcatc atagccaccg ccaccagtgg cgaaccaacg
     6961 gccttcggcg tactgatgcg cgagaccatc gagcagcagc gtcgcctggc ggtacgcgcg
     7021 agtcgtgagc cgcagatgcg cgagcgggtc gtagacatgc gtatcgcaac cgtgctgggt
     7081 gaccaggaag gtcggccgga acccctcagc gagcgcgggc aggacgcgct cgatcgccgc
     7141 gagccatgag ccgtcgccgg tcagcgggtg gagcggcagg ttgaccttgg tgccgagcgc
     7201 ctccggaccg ccgcgctcat cgacgaagcc gctgcccggg aagagcgtcc ggcccgtctc
     7261 gtgaacggac accgtcagga cgttggggtc atcccagaag agcgcttggg tgccgtcgcc
     7321 gtgatggacg tcgagatcga cgtacatgac gcgatgaccc gcgtcgcggg cccgcgccgc
     7381 tgctaacgcc acgtcgttgt agatgcaaaa gcccgacgcg cgaccggcca tggcatggtg
     7441 cagtccacca gccgggttga aggcgtggtc gacatcgccg cccaggatcc gatccacacc
     7501 ctcgagcgaa ccacccgcga cccgggcgga ggcctcgtgc atgtcttcga acggcgggca
     7561 atcgcccggg ccgatgcccg cggcgggcat gtggtgccac gggtcggcgc caaaacggcg
     7621 cacggtgttg atgtagtcgc gcgtgtgcag ccgggcgatc tcgtcgtcgg tggcgacgcg
     7681 cggctcaacg atgttggtgg cgcccagcgt ccggatcagg tcgatcgacg gcgggaagcg
     7741 gcgcggcgtc agcggatgct cggggccaaa gtcatatgtg agcgcggcgt cgccgtagac
     7801 gaggagcgaa ccggtcaccc cgcttctgac tccgcgtcga gctccggggc gggctccgcg
     7861 tcgagctccg gggcgggctc ccagccggtc gcccgcacgg cggcctgcca tgcatcgggc
     7921 gcggcctggc cgctcgccag ggccgcggcg tacgtgccga ccagccg
//
LOCUS       DATBRX010000195         2325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1864919, whole genome shotgun sequence.
ACCESSION   DATBRX010000195 DATBRX010000000
VERSION     DATBRX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864919"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VIF08_07570"
     CDS             complement(<1..453)
                     /locus_tag="VIF08_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135886.1"
                     /translation="MPKRRAKPDPVKTWIKRLDRTRPGLMTDTLAALATMYGRPTWGR
                     VYDPTSELILTMLSANSADIGAEKAFNQLCRRWPAPGSTVRGDAAAPIFRTGWGGVGI
                     SERSADWAAIEFADIGELTDVIRIGGLANQKAPRIQAALRQIREQRGDY"
     gene            complement(453..1478)
                     /locus_tag="VIF08_07575"
     CDS             complement(453..1478)
                     /locus_tag="VIF08_07575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8135887.1"
                     /translation="MRRARASDEAAVIEFASDTWDGWDYIPHAWPVWLEARDGVVLVA
                     CRPDDDTPIALTRIALVSQTEAWLEGIRVHPQLRGLEVASDLQVAELQWAAAQGANVI
                     RYATSARNEGSHRLGARHGIELLFELRNYWWSPDPDNDPDDPSAFDPAVRAAATALRG
                     RVLDALAAEGHVASPADTDRLWHIVAGDAGFLAAHRLYEPRPWALGELTAERFARHVE
                     RGEVIANSNANASAVAILTREQFAGEDSSLRLALLVGEPESVIALVERLRSIVDRTVR
                     FRVPEDDPRAPDLHERLVAAGYRSPDFRLHILGRPIDDEYPIPPVDQTRVVLGDEPQP
                     IVEPLAF"
     gene            complement(1517..>2325)
                     /gene="galE"
                     /locus_tag="VIF08_07580"
     CDS             complement(1517..>2325)
                     /gene="galE"
                     /locus_tag="VIF08_07580"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY8135888.1"
                     /translation="ATLREHSIDAVLHCAGYSLVGESVQQPQRYFDNNVARGIAFLDS
                     LLAAGVRRVVFSSSAAVYGVPDRIPIEETDTLAPVNPYGATKLALENALTSYATAYEW
                     RVVSVRYFNAAGATDKLGEDHRPETHLIPNILAAVERGHTVRIFGSDYPTPDGTCVRD
                     YIHVDDLAAAHIAALELTATRDAGHLACNLGSGSGFSNLEILRAAEQVVGHAIPHEIV
                     ERRPGDPPALVASNGRAREMLGWEPRHGSLEEVIGSAWAWRQRHPEGYTD"
BASE COUNT          347 a          782 c          825 g          371 t
ORIGIN      
        1 gtagtcaccg cgctgctcgc ggatctggcg cagcgcggcc tggatgcgcg gcgctttctg
       61 gttggccagg ccgccgattc ggataacgtc ggtcagctcg ccgatatcgg cgaactcgat
      121 cgcggcccaa tccgcactcc gctcgctgat gcctacgccg ccccagcctg tccggaagat
      181 gggcgccgcc gcgtcgccgc ggaccgtgct gccgggcgcc ggccagcgcc ggcacagctg
      241 gttgaacgcc ttttcggcgc cgatgtcggc cgagttcgcc gacagcatcg tcaggatgag
      301 ctcgctcgtg ggatcgtaga cgcgacccca ggtcgggcgg ccgtacatgg ttgccagtgc
      361 ggccagggtg tcagtcatca agccgggccg cgtcctgtcc aggcgcttga tccaggtctt
      421 cacgggatcc ggcttggcgc ggcgtttggg catcagaaag ccagcggctc aacgatgggc
      481 tgcggctcgt caccaaggac gacgcgggtc tggtcgacgg gtggaatcgg gtactcgtcg
      541 tcaatcgggc ggccaaggat gtgcagcctg aaatcgggtg atcggtagcc ggcggcgacc
      601 agtcgttcat gcaggtccgg cgcccgcggg tcgtcctcag gcacgcggaa gcgcaccgtt
      661 cgatcgacaa ttgagcgcag ccgctcgacg agcgcaatga cactctctgg ctcgccgacg
      721 agcagcgcca ggcggagcga tgagtcctcg ccggcgaact gctcgcgcgt caggatcgcc
      781 acggcgctgg cattggcgtt ggaattggcg atgacctcgc cgcgctcgac gtggcgcgcg
      841 aaacgctcgg cggtcagctc gcccagcgcc cagggccgcg gctcgtaaag ccggtgcgca
      901 gccaggaagc cggcgtcacc tgcaacgatg tgccacagcc ggtcagtgtc agcagggcta
      961 gcgacgtgac cttctgccgc gagggcatcg aggacgcgcc cgcgcagggc ggtcgcggcc
     1021 gcccgcaccg cggggtcaaa ggcgctcgga tcgtcggggt cgttgtccgg atcgggtgac
     1081 caccagtagt tgcgcagctc gaacagcagc tcgatgccgt ggcgcgcgcc cagccgatgc
     1141 gacccttcgt tgcgcgccga ggtggcatag cgaatgacgt tcgcgccctg ggccgctgcc
     1201 cactgcagct cggccacctg cagatcgctc gcgacttcca ggccgcggag ctgcgggtgg
     1261 acgcggatgc cctcgagcca ggcttctgtt tgcgagacca gcgcgatgcg cgtgagggcg
     1321 atcggcgtgt cgtcgtcggg gcggcacgcc acgagcacta cgccgtcgcg cgcctcgagc
     1381 cagaccggcc aggcgtgcgg gatgtagtcc cagccgtccc acgtgtcgct cgcgaactcg
     1441 atgactgcgg cctcgtcgct cgcgcgtgct cggcgcacga cgagcggcca atcgcgccgg
     1501 ccagcctgcg actcgctcag tcggtgtagc cctccgggtg gcgctgccgc catgcccacg
     1561 ccgagccgat cacctcttcg agcgacccgt ggcgcggttc ccagcccagc atctcgcgcg
     1621 cccggccatt gctggcaacc aatgccggcg gatcgcccgg cctgcgctcg acgatctcgt
     1681 ggggaatggc gtggccgacg acttgctcgg ccgcacggag gatttccagg ttgctgaagc
     1741 ccgagccgga gcccaggttg caggcaagat gcccggcgtc acgggtcgcg gtcagctcca
     1801 gcgccgcgat gtgggcggcc gcgaggtcat cgacgtggat gtagtcacgg acgcacgtgc
     1861 cgtctggcgt gggatagtcg ctcccgaaga tcctgaccgt gtggccgcgc tcgacggccg
     1921 cgaggatgtt cgggatgaga tgtgtctcgg gccggtgatc ctcgcccagt ttgtccgtcg
     1981 ctccggcggc gttgaaatag cgcaccgaga cgacgcgcca ctcataggcg gtcgcgtagg
     2041 acgtcagcgc attctcgagg gcaagcttgg tcgcgccgta aggattgacg ggcgcgagcg
     2101 tgtccgtctc ctcgatcggg atcctatccg gcacgccgta gaccgccgcg ctcgacgaga
     2161 acaccacgcg cctgacgccg gccgcgagca ggctgtccag gaacgcgatc ccgcgtgcga
     2221 cgttgttgtc gaaatagcgc tgcggctgct ggaccgattc acccacgagc gagtagccgg
     2281 cgcagtgcag aacggcgtcg atggaatgct cccggagtgt ggctt
//
LOCUS       DATBRX010000196        21121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2647017, whole genome shotgun sequence.
ACCESSION   DATBRX010000196 DATBRX010000000
VERSION     DATBRX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 21121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21121
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647017"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..832)
                     /locus_tag="VIF08_07585"
     CDS             complement(173..832)
                     /locus_tag="VIF08_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8135889.1"
                     /translation="MSEIDRKLRVMLVDDHALVRSAVRQAIAAPDVELVGEAATAQEA
                     MALAPVLRPDVLLLDIDLPGMNGIQLVQELAPRLPQTKIVMLTVSGSERDLLEAVARG
                     AAGYLTKDLSPEALLRSLRGTQRGELAMSRRFAARALQYFADAARRGRQVSGQEGELM
                     ALSPRENDVLAMLADGLTDREIAEALTISPRTVETHVSNILHKLDVRNRAEAAQRYRQ
                     G"
     gene            complement(829..2382)
                     /locus_tag="VIF08_07590"
     CDS             complement(829..2382)
                     /locus_tag="VIF08_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY8135890.1"
                     /translation="MDRLRERRDRLAIQQLDLVLLLTAGALTLLTFAFLTDPSLSFAL
                     LDRSTDVAVNSLTLLAAASLAALALARYRESGRLAGLFQSSAFLLLGWVAFVSIAISV
                     LKGEAQFGLSLGGEPEQLPIYISAIARLVGGAILVVAGAAAINIVRRAPRLRQTLLLP
                     TVTLSVIAAVLYLLRTQFPNIDSVLPPFIGPVGIQHMIDEPNFAGPIPDVTGLGALIQ
                     TVSVTVYLVGAFLYRRSYLRDGPVADGYLAVAMLIGAFAEIHFLFYPGVYGGLVTTSE
                     ALRLIFFVVLLLGINAESRSDLRALRTAYAALDRLRLSEAERATLEERTRLARELHDG
                     LAQDLWFAKLKHERLVAFVPEEQRPLAAEVTEALDSAIAEAKQAVVTMRAEDDRDVTL
                     EELLSRAVNAFAGRSGVRADFSAADLPTSLPARTQVEVLRILQEALTNVRKHADATVV
                     RVNAEVGDGHLQLSVVDNGRGFRPMETSGDGVGVQGMKERARLLGGELRIVSEPSAGT
                     ALHLTVPLT"
     gene            2731..3690
                     /locus_tag="VIF08_07595"
     CDS             2731..3690
                     /locus_tag="VIF08_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY8135891.1"
                     /translation="MRAPRASVQAPEWQIWLALGIVYIVWGSTYLAIRVMVETMPPLL
                     SGGVRHVVAGAIIFAILLLRRGPGALRLTPAEWVGGGLIGLALLLGGNGLVVLGERDV
                     PSGLTALIVAVVPLCVVVLRRIFGERIPLGTLIGVVAGFAGVAVLIIPEGVNGTVSVV
                     GMLMIVGASISWSVGTYFSRRLTLAQDPLASTGVQMLVGGTALLAVGALTGEAALVQP
                     QDFSTESLLSLGYLILFGSVLAYTAYTWVLIHASVSRVSTYAYVNPVVALFLGALLLN
                     EKVDATMLLGAAIIVVSVWLVIRTEARRSVRPAAALEAEPSQL"
     gene            complement(3677..4675)
                     /locus_tag="VIF08_07600"
     CDS             complement(3677..4675)
                     /locus_tag="VIF08_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY8135892.1"
                     /translation="MDPHDPGPEVAGRRAQDVLDAAVEPIALPDGATVRVGTASWTDP
                     TMTAGTVFYPKGADSAESRLQYYASQFPLVEIDATYYALPNARTAELWRDRTPPDFVF
                     DVKAHALMTGQPTETKRLPKVIREALPPELADKPRIYARDLPVELRQQVFTMFMEGLE
                     PLRAAGQLGSILLQYPRWFFPISESRDLILEAREMLDHTVAIELRNASWFNEKNVERT
                     LRFLTDNKLPFVMVDEPQGFKSSVPPVVAVSSPDLAVMRFHGRNAENWEKKGITPAER
                     FQYLYDREQLSEWAPRLRDAAKATRDVHVLMNNCYANYGATNARELARILADLKAD"
     gene            complement(4765..6135)
                     /locus_tag="VIF08_07605"
     CDS             complement(4765..6135)
                     /locus_tag="VIF08_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8135893.1"
                     /translation="MASLLDELRAELPDLRVLSDPADTESFRFDETAFLKAGYPLGVL
                     FPTSTAQVQAIVRAAATAGVPIVARGAGTGLSGGAVAVDGALTVVFTKMNAILEIDSA
                     NLTATVQPGVINADLGKAAGEQGLFYPPDPASYETCTIGGNVAENSGGLRCVKYGVTR
                     DWVCGLEVVLADGSVIRTGGKNKKDVAGYDMTGLFVGSEGTLGLVTEVTVRLLPLPPA
                     KLTLIAFFATVHDAGEAVAGITAAGVVPVTLELMDAYTIAAVDDAFGLGLDRAAGAML
                     MVESDLGGAAAEAQLLAVEAACSAARATSVLRAADAQEADWLRQARREAHGSLERRGA
                     ARMDDVSVPRDRVPDMIDAITRIAAANELPVGIFGHAGDGNLHPTYILDRDDPTAEER
                     IDKTRDQIYRAALEMGGAITGEHGTGLAKKKWLELQRGARSVEVMRSIKAALDPHGLL
                     NPGKVF"
     gene            6169..7368
                     /locus_tag="VIF08_07610"
     CDS             6169..7368
                     /locus_tag="VIF08_07610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8135894.1"
                     /translation="MIRLAIALFLVQAGFHGFTASIPVALARAGRSDAEIGAIVGIAS
                     LVMIGGALAGGALIDRFGTMRLFIAGGLMYISAALILIVGGLDSTVGVVAARVLQGGA
                     SGLTVPASMALVPSLVSAARRGVAIATVGSAHSLTMVLLPSISIVVLDLYGMSGVSLV
                     VAGFVVAALAIVVARPIRPNEAAPSHLAAARRRLGFAWRRSWLVPLAVIVLFVLHWGL
                     LIAYLPQRAEAAHAEIGLFFAADGIAVLAARIPAGWLADHMPPVGPVLAGVALTFAGV
                     APLFEPPTTPVLIVAGLLTGIGGALIIQPLMLAMTARSTDADRGSAFALFSASFSASV
                     ALASVGTAPLIGSVGYATLLAVALGALGLSAVVALLDADLRRRPHVDDDPAQAVALAK
                     EANAIGP"
     gene            7465..8607
                     /locus_tag="VIF08_07615"
     CDS             7465..8607
                     /locus_tag="VIF08_07615"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY8135895.1"
                     /translation="MKIGVVKERAPNERRVALVPEALKPLLGAGAEILVERGAGDGAA
                     LPDSLYEAAGAKVVDHATLYKEADVIARVQKPDADDAKALRQGQVVVGLLQPLLDPGL
                     MKSLADGGVTAISLDAIPRTLSRAQSMDVLSSQANVGGYRAVLIAAEAYGRYFPLLTT
                     AAGTARPANVLILGVGVAGLQAIATARRLGAIVKAYDVRSETKEQAESLGAGFIVLKS
                     VADATGAGGYARELTTEERAAQQQELNGHIGGMDVIITTAQVPGRRPPLLVTADAVKG
                     MKPGSVIVDMAASALGGNVELSKPDEKVVTDNGVTILAPTNLPATMPAGASQFYARNL
                     AAFLLNFMKDGKLNIDLSDEISAATVITHDGAVVQEATRKLLEGAQ"
     gene            8604..8897
                     /locus_tag="VIF08_07620"
     CDS             8604..8897
                     /locus_tag="VIF08_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY8135896.1"
                     /translation="MSPELLNAIAIFVLAILVGFEVISKVPATLHTPLMSGANSIHGI
                     VMVGAMLIAAAADNWLSYTLAFVAMVFGAANVVGGYVVTDRMLQMFRKRDPKS"
     gene            8894..10357
                     /locus_tag="VIF08_07625"
     CDS             8894..10357
                     /locus_tag="VIF08_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY8135897.1"
                     /translation="MSQPTIDALVFLAWLVGAAGFVLGLHQMNSPATARRGNQLSAAG
                     MALAVVVTFLWLLTPGNPIRPGGMGIEAIAIILVGFVLGGGIGLWMARKVAMTAMPQL
                     VSLFNAVGGGAAALVAIDDFIRIVGTPELSISTAIFVVLGAVIGSVTFSGSVIASGKL
                     QGIVSGAPITFPGSRLITAITALGAAAGLVVLVAGAAGLLTLSAETQLITLGLIVVAA
                     LIFGVTLVLPIGGADMPVVISLLNSFTGTAAAMAGFVIGNPVLIISGALVGASGAILT
                     KLMADAMNRSIGNIMVGGFGTGDSAGGEGGPGSTSSVREIGVDDAAIQLAYAQKVIIV
                     PGYGLAVAQAQHACRELADLLEDRGIEVSYAIHPVAGRMPGHMNVLLAEANVPYPQLK
                     EMDEVNPEFERADVALVVGANDVTNPAARTNKSSPIYGMPILDVDKARSIIVIKRSMR
                     PGYAGIDNELYGDPKTGMLFADAREGLSALTAAVKEL"
     gene            10378..12336
                     /locus_tag="VIF08_07630"
     CDS             10378..12336
                     /locus_tag="VIF08_07630"
                     /inference="COORDINATES: protein motif:HMM:NF041501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGLLP motif-containing membrane protein"
                     /protein_id="HEY8135898.1"
                     /translation="MAGRVAVARIISALMLFSVALLLMPARAVAATAVITSPGPLSQI
                     FISDTLACQVAYAGDEGFEFFPSTSEEGDCGTWVALGGSVWGPAGGSATLTPYTTISQ
                     SPVTGSGTQNDPLHVVTVVSIPEAGLRITETDSYAVGSRAYRTDVQIENIGNAIQTGV
                     LYRAGDCYLQGSDAGFVRVEGGSPACIVDPALGRRVEQWLPITVGSHYFAGYYGEVWT
                     NIGNQVPFPDTCACTEQFTFDNGAGISWPFNVAPGQSATFSHETFFSPVGGSPTSGIP
                     NSFRDSVPTQITLDPIVVAQSIAITAGVIVLVPFPSALFNSTLEDNYAEVMGWLAGIK
                     ARLNGLWSRFVAWVRGQVASRRAPAGGSPGPDAVAASAPDAAGLSAPDAAGLSSAAPA
                     PATDPWRSPLRIAGFVLLTALLYAFLDPTFGFSLDSLATFTGLAMGLFVVLLAYGVPL
                     LILSRRDGFTLTVRALPATLFIGLLCVLVSRIADFQPGYLYGLIVGFFFAHGVERRLE
                     GRAEAVAAGTSLLAALIAWIVLAFLRGSGSQPGDLFPVVLEAAMVTVVVAGLENAVFA
                     MLPLRFMPGAAVYAWDRRVWVALLALGLFGFAHVLLNPAEGAGYLADTTRTSFFTLIL
                     LLAVFGVSSVLFWAYFRFRPARKAAPAE"
     gene            12341..12757
                     /locus_tag="VIF08_07635"
     CDS             12341..12757
                     /locus_tag="VIF08_07635"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8135899.1"
                     /translation="MTDRVELQLEVDAARTAVYELFATEAGLRRWLDAAELEPRVGGA
                     LRVRLRDATASGKVLALDPPQHASFTWEWSGQRDAPPMVVAFDAIDHGSRTHVTLRHV
                     GLHTRAESDLHHELWRHWLERFEEAVRELPREVVPG"
     gene            complement(12782..13774)
                     /locus_tag="VIF08_07640"
     CDS             complement(12782..13774)
                     /locus_tag="VIF08_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY8135900.1"
                     /translation="MSVANEHFGKWDRAARYLKIAMILKDYPDGISAQALADRIGVSK
                     RTVYRDVLAMDEQAGLPIWNDEGKFGLDKGAFLQPLALTMHEATTLFLAARMLAKASD
                     EHDTELIGAFVKLASILPPVLAEHIQATVDAFSTTPPNDRFTRVFRVLAEAWAKRRVV
                     QIEYDSGVYDPKKGPRKTRVRPLAIEPSALTHALYLIGYDESRKARRTYKVERILSAS
                     LTPETFTPPESESPAHELLKAWDVIADEDPVKVVIRFSPAVAKRAAETRWHPSQVLEP
                     QDDGSLLWRGQIAGMREIRIWILGWGADAEVLEPAVLRDEVAAELKKAATLYRG"
     gene            complement(13820..16546)
                     /locus_tag="VIF08_07645"
     CDS             complement(13820..16546)
                     /locus_tag="VIF08_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY8135901.1"
                     /translation="METATTATSNTQLLTVSDAARMLGVHANTVRSWTDQGVLACLRI
                     NSRGDRRYARQEIARFLAQTEGIAPTQTANPTNGLLTRSAALAVRKTDARSAAESVVE
                     MLCKEKGYTGAALVAPDSSSRLVFGAIEPDERLAARAAERHAVLMTTPRRRDGRYQAA
                     LPFGDGQAGGGVVLLAGTATTRDHEEPQLLAAVAAQLDVAVALSGQIERLDRRHHRAE
                     LLFSIDDDLGARRDPDRVLAQLVEKAVHAFGAQHAGVFLTGADGSFKAKVTRNLSAEY
                     CQILEHATTRPLTTLSLVERRAVTAADASDDPRYFELRTAIRREGFNTIAVAPLITED
                     KAFGVLCLYHDERHEWSEADLALLERLARHGAAVMRSAQDYSQMAKWTAQLQSIQQLG
                     ARLTRLRSVAEVGQAICAELVQLIDTHNTRVYRVIGGDCEPVAWRGQIGEYDSEDSEQ
                     LRLKVGEGMTGWVARFGLAQNVGDAAHDKRALTIPGTEDDLDESLLLAPMLYEDNVLG
                     VIVLAKLGLNQFTADDLRLLEIYASIAAQAMANADATEQLHAQSETLARQVSSQRELL
                     RVTESILGTLDTQALLEEIAERLNTLVEVDNLSVMLHDATTDRLNTIFAVGVHAEKVR
                     QLSLPTDDSVSGNVLRNGEAVLVSDELAEPRLARPYGLEPRPGALIVAPLRNGERVHG
                     VLALERLGGEAAFTNEEFELVKLFAAHVSIALRNAAQHRAVELRAETDRLTGLWNHGA
                     LTEHLERLVERGARFGMLMVDLDRFKEYNDQNGHQAGNVMLQRVADRLRASCRDSDLV
                     FRFGGDEFALLLPGTGLPGARTVAAKVNEAIAAIEGQNDLGVTCSVGVAAFPRDARDA
                     ASIILAADRACYAAKRSGRNRIATAAEGLALASDFQPTEPTPMEPQPAFSAA"
     gene            complement(16690..17664)
                     /locus_tag="VIF08_07650"
     CDS             complement(16690..17664)
                     /locus_tag="VIF08_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135902.1"
                     /translation="MPWSIVLAGGGAFALVAALLLLRSLGTRFRLGRLLSAAPEVTLE
                     ECRELAAGAAVYVRVSGRISSTEEFPDDNDRPLVFRRTRLEVQVGPDRWRTVLDEREA
                     VPFGVESRSAFVAIDQDALGEGLVAIPRVATGRASELHGDMAAAASGVAPDSPVRLTI
                     EQLSAVEHVSACGTPQLLDGEPVLSAGLGRPLIVTALDRPAAMRLLASGARRRVAAAA
                     VLIATGLALVAIAVGVAFLATALAEPVLAASPSLLAASPSPLPSPEPSMVIGVPTDPR
                     AGGGAPLVGAPGLAFLVVVGAGLLAALVTLAYVRLAGARSTSGPAKRR"
     gene            complement(17897..18475)
                     /locus_tag="VIF08_07655"
     CDS             complement(17897..18475)
                     /locus_tag="VIF08_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135903.1"
                     /translation="MSLPRTEPERLSVQYPGGIAARYRWTGSGGGPAIFAISEAVGTL
                     TDHGPLVEPDPDRLCRLELHVESPGGGWTARFASPIYDEPRGALWDEAGLLLVGYGFH
                     LYALEPRTGELAWTHRSGSPVVAVIASSRLEHVLLQTEIETIALRREGEVAWRAAHSD
                     VIVEAALIAGRLDLTTYGGAHVYLDAASGRAA"
     gene            complement(18486..19022)
                     /locus_tag="VIF08_07660"
     CDS             complement(18486..19022)
                     /locus_tag="VIF08_07660"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY8135904.1"
                     /translation="MSPVQPAVQRVLDAAARKGVQLQVKEFDRSTHTAAEAAAAVGAE
                     VGQIVKSLVFVADGTDGVHACLVLASGANTVDVGLLASALTEPNIRRATADEARELTG
                     FSIGGIPPFGHIAPIRTLMDPDLGRFELVWAAAGTPNAVFAVTPNELRVLANAIVTPI
                     SAGRAPGTEPLSANAGTA"
     regulatory      19074..19181
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            19209..19874
                     /locus_tag="VIF08_07665"
     CDS             19209..19874
                     /locus_tag="VIF08_07665"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEY8135905.1"
                     /translation="MVVALIVADGDVPARSDVERLLGGVPQLVIAADGGALKAQALGY
                     PARVVVGDADSLAPAHVEQLRAARAEVVIHPAAKDESDTELALEEALSRGATRVVIIG
                     AFGGKRLDHTLANILLLASERLAGRDICLADGASTLRVMHDAERLAITGEQGDYVSLL
                     PLTAHVSGVSTAGLRYALADETLHQGPARGLSNELTDAGATIQIDEGRLAVIHTRRSE
                     VSD"
     gene            19874..20989
                     /locus_tag="VIF08_07670"
     CDS             19874..20989
                     /locus_tag="VIF08_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8135906.1"
                     /translation="MNESNRPVLLTIAALAVVVVIVVAALSGVFTTPNPDAAGTPTPP
                     VFTAPPTQLTLLTHDSFAVTPEILAAFESANNARVNVLRAGDAGQMVNQAILTRDDPL
                     ADLLYGVDNTFLSRALDADIFEPYRAAGVTAVPAVLRLDARERVTPIDYGDVCINFDK
                     EAFDDANPAPRTLADLAKPQYSGQLVVEDPGTSSPGLAFMLATIAEFGESGVYTWLDY
                     WADLRDSDVAIVSGWSEAYESTFSGGSGRGDRPLVVSYATSPVAEVFYASPQPAEAPT
                     GVMTDGCFRQVEFAGVLADTAQPELARKFIDFMLTAEFQADIPLNMFVFPAVPATNIA
                     QVFRDYAVVVPEPLTMAPEQIGLNRERWLNEWQAATH"
BASE COUNT         3127 a         7271 c         7205 g         3518 t
ORIGIN      
        1 tagggtggac gacgtgccga cgggtcatct gagcccaggg gctctggctg gggcgttgcc
       61 tgcgccgccg tccacctcgc gcatcatcgc gcactcgtca agtgccattc ccgctgggca
      121 gcggcttcca cgctgagtga tctatctcaa atcggagtag cgctacctcc ggctagccct
      181 gccggtagcg ctgggcggcc tcggcgcggt tgcgcacgtc gagcttgtgc aggatgttgc
      241 tgacgtgcgt ctcgacggtg cgcggcgaga tcgtcagcgc ctcggcgatc tcgcggtctg
      301 tcagcccgtc ggcgagcatt gccagcacgt cgttctcgcg cggcgatagc gccatcagct
      361 cgccttcctg gccgctaact tgccgcccgc gccgcgcggc gtcagcaaag tactggagcg
      421 cgcgggcggc gaagcggcgt gacattgcga gctcgccgcg ctgagtgccg cgcagcgagc
      481 gcagcagcgc ttccggcgac agatccttcg tcagatagcc ggcggcgccg cgcgcgacgg
      541 cctcgagcag gtccctctcc gatccggaca cggtcagcat cacgatcttg gtctgcggca
      601 gacgcggcgc cagctcctgg acaagctgga tgccgttcat gcccggcagg tcgatgtcga
      661 gcagcagcac gtccggtcgc agaacgggcg ccagcgccat cgcctcctgg gccgtggccg
      721 cctcgccgac cagctcgacg tccggcgccg cgatcgcctg gcgcacggcg gagcgaacga
      781 gcgcgtgatc gtcgaccagc atcacccgca gcttgcgatc aatctcgctc acgtcagcgg
      841 caccgtcagg tgcagcgcgg tcccggccga cggctctgaa acgatgcgca gctccccgcc
      901 caacagtcgt gctcgctcct tcatgccctg cacacccact ccatcaccgc tcgtctccat
      961 cggacggaag ccgcgaccat tgtcaaccac gctgagctgc agatggccgt ccccgacctc
     1021 cgcgttgacc cgcaccaccg tcgcatcggc atgcttgcgc acgttggtca gcgcctcctg
     1081 caggatgcgc agcacctcga cctgggtgcg cgccgggagc gaggtcggca ggtcggcggc
     1141 ggaaaagtcg gcccgcacgc cagagcggcc ggcaaacgca ttcacggcac gcgagagcaa
     1201 ctcctcgagc gtcacgtcgc gatcgtcttc ggcgcgcatc gtgaccaccg cctgcttggc
     1261 ctccgcgatg gcggagtcga gcgcctccgt tacctcggcc gcgagcggac gctgctcttc
     1321 gggcacgaat gccaccagcc gctcgtgctt cagcttggcg aaccacaggt cctgcgccag
     1381 gccgtcgtgc agctcacgcg cgagacgcgt ccgttcttcg agcgtcgccc gctccgcctc
     1441 ggacagtcgc aaccgatcga gggctgcgta ggcggtgcgc agcgcacgca ggtcggagcg
     1501 tgattcggca ttgatgccca gcagcagcac cacgaagaag atgagccgca gcgcctcgct
     1561 cgtcgtgacc aggccgccat agacgccggg gtagaacagg aagtggatct ccgcgaaggc
     1621 gccgatgagc atcgccaccg ccaggtaccc atcggccact gggccatcgc gcaggtacga
     1681 gcgccgatac aggaatgcgc cgaccaggta gaccgtcacg ctgaccgtct ggatcagtgc
     1741 tccgaggccg gtgacgtccg gtatcggtcc cgcgaaattg ggctcgtcga tcatgtgctg
     1801 gatgccgacc gggccgatga acgggggcag cacgctgtcg atgttgggga actgcgtgcg
     1861 cagcaggtag agcacggccg cgatcacgct cagcgtcacc gtcggcagca acagcgtctg
     1921 gcgcaggcgc ggcgcacggc gcacgatgtt gatcgccgcc gcgccggcca cgacgaggat
     1981 ggcgccgccg accaggcggg cgatggccga gatgtagatc ggcagctgct cgggctcacc
     2041 gcccagcgac aggccgaact gcgcctctcc cttgagcacg ctgatcgcaa tgctgacgaa
     2101 cgcgacccag cccagcagca ggaaggcgct gctctggaac agtccggcca atcggcctga
     2161 ttcgcggtag cgcgccagcg cgagcgcagc gaggctggcc gccgcgagca gggtcagtga
     2221 gttgaccgcg acatcggtcg agcgatcaag cagggcaaag gacagcgacg gatcggtcag
     2281 gaacgcgaac gtcaagagcg tgagcgcgcc ggcggtcagc agcagcacga ggtcgagctg
     2341 ctggatggcc aaccgatcgc gccgttcgcg cagcctatcc atgcgccgat gataggtcgc
     2401 gggccggcgc tgcccgagcg aaatgacgct ccgaaaaatg cgccgggaaa gactagagcc
     2461 ggactgcgcc ccagcgcccg tccggctccg tcatcgcttg tcgtcacacc ccgccgtgta
     2521 agggtggagc caagcttgaa tgttcaattt cggcagccac cagtggccga ccgagacaaa
     2581 tcatcgggtc cacgctcatt tcgcaccatc cggactggca cggaattgag tacgtagcct
     2641 gatctacgta ctagaggcac cggacgatgg ggtcccgacc cttgcctacc agtccgcccg
     2701 cgatcgctcc ttttcccgca aactccaccg gtgcgcgcac cacgagccag tgtccaggca
     2761 cccgaatggc agatctggct ggcccttggg atcgtctaca tcgtctgggg ctcgacctac
     2821 ctcgcgatcc gcgtcatggt cgagacgatg ccgccgctgc tctccggcgg cgtccgccac
     2881 gtcgttgccg gcgcaatcat ctttgccatc ctgttgctcc ggcgcggacc cggcgcactc
     2941 cgcctgacgc ccgccgaatg ggtaggcggc ggactcatcg gactcgccct gctgctcggc
     3001 ggcaacggcc tcgtggtgct gggtgagcgc gatgtgccca gcggactgac ggcgctgatc
     3061 gttgccgtcg ttccgctctg cgtggtcgtg ctgcggcgca tctttggcga acgcatcccg
     3121 ctgggcaccc tgatcggcgt ggtcgcaggc ttcgccggag ttgcagtgct gatcatcccc
     3181 gagggtgtga acggcaccgt cagtgtggtc gggatgttga tgatcgtcgg agcttcgatc
     3241 agctggtcgg tcgggaccta cttctcgagg cggctgacat tggcccagga tccgctggcc
     3301 tcgaccgggg tccagatgct cgtcggcggt accgcgctgc tggccgtggg cgctctgact
     3361 ggcgaagccg cgttggttca gccgcaggac ttcagcaccg agtcgctgct ttcgttgggc
     3421 tacctgatcc tcttcggaag cgtgctcgcg tacacggcgt acacatgggt gctgatccat
     3481 gcaagcgtgt cacgcgtctc gacctatgcg tatgtgaacc cagtggtcgc gctcttcctt
     3541 ggggctctgc tcctcaacga gaaggtcgac gcgacgatgc tgctgggtgc cgcgatcatc
     3601 gtggtatcgg tgtggctggt catccgcacc gaggcgcgtc gttcggtgcg gcccgcggcg
     3661 gcgttagagg ccgaacctag tcagctttga ggtcggccag gatgcgcgcc agctcgcggg
     3721 cgttggtcgc gccgtagttg gcatagcagt tgttcatcag cacgtggacg tcgcgcgtcg
     3781 ctttcgccgc gtcccgcagc cgcggcgccc actccgatag ctgctcgcgg tcgtagaggt
     3841 actggaaccg ctcggccggg gtgatgccct tcttctccca gttctccgcg ttgcggccgt
     3901 ggaagcgcat gacggccagg tccggtgaag aaaccgcgac cacgggcggg acactgctct
     3961 tgaagccctg gggctcatcg accatgacga acggcagctt gttgtcggtc aggaagcgca
     4021 gggtccgctc gacgttcttc tcgttgaacc aggacgcgtt gcgcagctct atcgcgaccg
     4081 tgtggtcgag catctcgcgt gcttcgagga tcaggtcccg actctcactg atgggaaaga
     4141 accagcgcgg gtactggagc aggatcgagc ccagctgccc cgcggcacgg agcggctcca
     4201 gaccttccat gaacatggtg aagacctgct ggcgcagctc gaccgggagg tcgcgcgcgt
     4261 agatccgcgg tttgtcggca agctccggcg gcagtgcctc gcggatgacc ttgggcagac
     4321 gcttggtctc agtcggctgg ccggtcatca gggcgtgcgc cttgacgtcg aagacgaagt
     4381 cgggcggcgt ccggtcgcgc cacagctccg ctgtccgcgc gttgggcagg gcgtagtagg
     4441 tcgcgtcgat ctcgaccagc gggaactggc tggcgtagta ctggagccgg ctctccgcgg
     4501 agtcagcgcc cttgggatag aagaccgtgc cggcggtcat cgtcggatcc gtccacgacg
     4561 ccgtgccgac gcgcacggtc gcgccgtcgg gcagcgcaat gggctcgacc gccgcgtcga
     4621 ggacgtcctg tgcgcgcctg cccgcgacct ccggccccgg atcgtgcgga tccacctgaa
     4681 cagtctcctc cgcagtgcaa gcactgctgc gcgcgacagt ggtccggctc cgacggatcg
     4741 ccggtgccgg cccgtcgctt gcggttagaa caccttgcct gggttgagca atccatgcgg
     4801 gtcgagcgcc gctttgatcg agcgcatcac ttccactgac cgcgcgccgc gctgcaactc
     4861 cagccacttc ttcttggcga gaccggtgcc gtgctcaccc gtgatggcgc cgcccatctc
     4921 cagggccgcg cggtagatct ggtcacgcgt cttgtcgatg cgctcctcgg cggtcgggtc
     4981 atcgcggtca aggatgtacg tcgggtgcag gttgccgtcg ccggcgtggc cgaagatgcc
     5041 gaccggcagc tcgttggccg cggcaatacg cgtgatcgcg tcgatcatgt cggggacgcg
     5101 atcgcgcggc acgctgacgt cgtccatgcg cgcggcgccg cgccgttcga gcgagccgtg
     5161 cgcctcgcgt cgcgcctggc gcagccagtc cgcttcctgg gcatccgcgg cgcgcaacac
     5221 agaagtggcg cgggcggccg agcatgccgc ctccaccgcg agcagctgcg cctccgcggc
     5281 cgcgccaccc agatcggact cgaccatcag cattgccccg gcggcgcggt cgaggcccag
     5341 cccaaaggca tcgtcgaccg cggcgatcgt gtacgcgtcc atcaactcga gcgttaccgg
     5401 gacgacgccc gcggcggtga tcccggccac cgcctcgccc gcgtcgtgga cggtggcgaa
     5461 gaacgcgatg agcgtcagct tggccggcgg cagcggcagc agccgcacgg tcacttccgt
     5521 cacgaggccc agcgtgccct ctgagccgac gaacagcccg gtcatgtcgt aacccgcgac
     5581 gtccttcttg ttcttgccgc cggtgcgaat gaccgatcca tcggcgagca cgacctccag
     5641 cccgcacacc cagtcacgcg tcacgccgta cttgacacag cgcaggccgc ccgagttctc
     5701 ggccacgttg ccgccgatgg tgcaggtctc gtagctcgcc ggatcaggcg ggtagaacaa
     5761 gccctgctcg cctgccgcct tgcctagatc cgcgttgatg acgcccggct ggacggttgc
     5821 ggtcaggttc gcgctgtcga tctcgaggat cgcgttcatc ttcgtgaaga cgaccgtcag
     5881 cgctccgtcc accgcgacgg cgccgcccga caggcccgtt ccggcaccgc gcgcaacgat
     5941 gggcacgccc gcggtagccg cggcgcggac gatcgcctgg acctgcgccg ttgacgttgg
     6001 aaacaggacg cccagcggat agccggcctt caggaaggcg gtctcgtcga agcggaagga
     6061 ctccgtgtcg gccgggtcgc tcaggacgcg caggtcgggc agctcagcgc gcagctcatc
     6121 aagcagggac gccatcgcgg tagaggctaa tcggcgatca tcggcggcgt gatccgcttg
     6181 gcgatcgccc tcttcctggt ccaggccggc ttccatggct tcacggcgtc gattccggtc
     6241 gcgctcgcgc gtgccgggcg atccgacgcc gagatcggcg cgatcgtcgg gatcgcttca
     6301 ctcgtcatga tcggtggggc gctggccggc ggagcgctga tcgaccgttt tggcaccatg
     6361 cgcctgttca ttgccggcgg gctgatgtac atctcggccg cgctgatcct gatcgtcggt
     6421 ggtctcgact cgaccgtggg agtcgtcgcc gcgcgtgtcc tccagggcgg cgcaagcggt
     6481 ctgaccgtgc ccgcgtcgat ggccctcgtc ccgtcactgg tctccgccgc gcgacgcggt
     6541 gtggccatcg caacggtggg ctcggcgcac agcctgacaa tggtgctgct gccatcgatt
     6601 tcgattgtgg tgctcgactt gtacggcatg agcggcgtca gcctggtggt cgccggcttc
     6661 gtcgttgcgg cgctggcgat cgtcgtcgcc cgacccatca ggccgaacga agcggctccc
     6721 tcccatctgg ccgcggcccg gcgccggctt ggcttcgcct ggcgccgcag ctggctggta
     6781 ccgctcgctg tgatcgtcct gttcgttctg cactggggac tgctgatcgc ttacctgcca
     6841 cagcgggccg aggccgccca cgccgagatc gggctcttct ttgcagccga tggcatcgcc
     6901 gtgctggcgg cgcgcatacc cgccggctgg ctggccgacc acatgccgcc ggtcggcccg
     6961 gtcctggcgg gggtcgcgct gaccttcgcc ggagtggcgc cgctcttcga gccgccgacg
     7021 acaccggtgc tcatcgtggc cgggctgctg acgggtatcg gcggagcgct gatcatccaa
     7081 ccgctgatgc tggcgatgac cgcgcgcagc acggacgcgg accgcggcag cgccttcgcc
     7141 ctgttttcgg cgtccttctc cgcgtcggtc gcgttggcga gcgtcggcac ggcgccgctc
     7201 atcggcagcg tgggttacgc gactttgctg gcagtagccc tgggcgccct ggggctgtcc
     7261 gcggtcgtgg cgctgctcga cgccgacctg cgccggaggc cgcacgtcga cgatgacccg
     7321 gcgcaggccg tagcgctcgc caaagaagcc aacgcgatcg ggccgtagta ccggggcggg
     7381 tggggccatt tgccatgcga caggttggca acggccgact attcttcgag caattacacg
     7441 accatccgtt gcccaggggt cccgatgaag attggtgtgg tgaaagagcg cgcgcccaat
     7501 gagcggcgtg tcgcgctcgt tcccgaagca ctgaagccgc tgcttggtgc gggcgctgaa
     7561 atcctggtcg aacgcggcgc cggcgacggc gcggccctgc cggacagcct gtacgaggcc
     7621 gccggcgcca aggtcgtcga tcacgcgacg ctctacaagg aagcggacgt gatcgcccgc
     7681 gtccagaagc ccgacgcgga cgatgccaag gccctgcgtc agggccaggt tgtggtcggt
     7741 ctgctccagc cgctgctcga cccggggctc atgaagtcgc tcgctgacgg cggtgtcacc
     7801 gcgatcagcc tcgacgcgat cccgcggacg ctgtcgcgcg cgcaatcaat ggatgtgctc
     7861 tcgtcgcagg cgaacgttgg tggttaccgc gcggtactga tcgcggcgga ggcctacggg
     7921 cgctacttcc cgctcctcac taccgccgcg ggcacggccc gaccggccaa cgtgctgatc
     7981 ctgggtgtcg gcgttgccgg cctccaggcg atcgccaccg cgcgccgctt gggcgcgatc
     8041 gtcaaggcct atgacgtgcg ctccgagacg aaggaacagg ccgaatcgct cggcgcgggg
     8101 ttcatcgtgc tgaagtccgt cgccgacgcc accggcgccg ggggatacgc gcgcgagctg
     8161 accaccgagg agcgagccgc ccagcagcag gagctgaatg gccacatcgg cggcatggat
     8221 gtgatcatca cgaccgccca ggtgcccggc cgccgcccgc cgctgctcgt gacggccgac
     8281 gcggtcaaag gcatgaagcc cggctcggtc attgtcgaca tggcggcctc cgcgttgggc
     8341 ggcaacgtcg agctttccaa gcccgatgag aaagtggtca ccgacaacgg tgtgacgatc
     8401 ctggcaccga ccaacctgcc ggccacgatg cccgcaggcg ccagccagtt ctacgcgcgc
     8461 aacctggcgg cgttcctgct caacttcatg aaggacggca agttgaacat cgatctgagc
     8521 gacgagatca gcgccgcgac cgtgatcacg cacgatggcg ccgtggtcca ggaagcgacc
     8581 aggaagctgc tggagggcgc gcagtgagcc ccgagctgct caacgcgatc gcgatcttcg
     8641 tcctcgccat cctcgtcggc ttcgaggtca tcagcaaggt cccggcgacg ttgcacacgc
     8701 cgctcatgtc gggcgccaac tccatccacg ggatcgtcat ggtgggcgca atgctcatcg
     8761 cggcggcggc cgacaactgg ctctcctaca ccctcgcctt cgtggccatg gtgttcggcg
     8821 ccgccaacgt cgtcggcggc tatgtcgtga ccgaccggat gctccagatg ttccgcaagc
     8881 gagatcccaa gtcatgagcc agccgacgat cgacgccctg gtcttcctcg cctggctggt
     8941 cggcgcagcc ggcttcgtcc ttggcctgca ccagatgaac tcgcccgcca ccgcgcggcg
     9001 cggcaaccag ctgagcgcgg ccggcatggc gctcgccgtt gtggtcactt tcctgtggct
     9061 gctgacgccg ggcaacccga tccggccggg cggcatgggc atcgaggcga tcgcgatcat
     9121 cctcgttggc ttcgtgctcg gtggcggcat cggcctgtgg atggcgcgca aggtcgccat
     9181 gacggcgatg ccgcagctcg tctcgctctt caacgcggtc ggtggtggcg cggctgccct
     9241 cgtcgcgatc gacgacttca tccgcatcgt gggcacgccc gagctgtcga tctcgacggc
     9301 gatcttcgtg gtcctgggcg cggtcatcgg ctcggtcact ttctccggct cggtcatcgc
     9361 gtcgggcaag ctgcagggca tcgtctctgg tgcgcccatt accttcccag gctcgcgcct
     9421 gatcacggcg atcaccgccc ttggcgcggc cgccggcctg gtcgtgctcg tggctggcgc
     9481 cgccggcctg ttgacgctca gcgctgagac gcagctcatc acgctaggcc tgatcgtcgt
     9541 tgcggcgctg atcttcggtg tcaccctggt gctgcccatc ggcggcgcag acatgccggt
     9601 cgtcatctcc ctgctcaact cgttcaccgg cacggctgct gcgatggccg gcttcgtcat
     9661 cggcaacccg gtgctgatca tctccggcgc actcgtcggt gcttcgggcg cgatcctgac
     9721 caagctcatg gcggacgcca tgaatcggtc catcggcaac atcatggtcg gcggctttgg
     9781 cacgggcgac agtgctggtg gcgagggcgg cccgggttcg acgagcagcg tccgcgagat
     9841 cggtgtcgac gacgccgcga tccagctggc atatgcccag aaggtcatca tcgtgcccgg
     9901 ctacggcctg gccgtggccc aggcgcagca cgcctgtcgc gagctcgccg acctgctcga
     9961 ggaccgcggg atcgaggtct cgtacgccat ccatcccgtc gcggggcgca tgcccggcca
    10021 catgaacgtg ctgctggcgg aggccaacgt gccgtacccg cagctcaagg agatggacga
    10081 ggtcaacccg gagttcgagc gtgccgacgt cgcactggtc gtgggcgcga acgacgtgac
    10141 caaccccgcg gcgcgcacca acaagtcgtc gccgatctac gggatgccca tcctcgatgt
    10201 cgacaaggcg cgcagcatca tcgtgatcaa gcgatccatg cggcccggct acgcgggcat
    10261 cgacaacgag ctctacggcg atcccaagac gggcatgctc tttgccgacg cgcgcgaggg
    10321 cttgagcgcg ctgaccgccg cagtgaagga gctctagcac tcttactatt tcggcccatg
    10381 gcgggtcgag tggcggttgc ccggatcatc agcgcgctga tgctgttcag cgtcgcgctg
    10441 ctgttgatgc cggcgcgcgc ggtggcggcg accgctgtca tcacttcgcc cggcccgttg
    10501 agccagatct tcatctccga caccctggcc tgccaggtcg cgtacgcggg cgacgaaggc
    10561 ttcgagttct ttccgtccac ctcggaagag ggcgactgcg gcacgtgggt ggccctaggc
    10621 ggcagcgtgt ggggtccggc cggtggctcc gcgacgctga ccccttacac gaccatcagt
    10681 caatcgccgg tgaccggctc aggcacccag aacgatccgt tacacgtcgt caccgtcgtt
    10741 tcgattcctg aggcggggct gcggataacc gagaccgaca gctatgccgt cggcagtcgc
    10801 gcataccgga ccgacgtcca gatcgagaac atcggcaacg ccatccagac gggcgtgctg
    10861 taccgcgccg gcgactgcta cctgcagggc agcgacgccg gcttcgtgcg tgtcgaaggt
    10921 ggttctccgg cgtgcatcgt cgacccggca ctgggccgcc gcgtcgagca atggctcccg
    10981 atcaccgttg gcagccacta tttcgcgggc tactacggcg aggtatggac caacatcggc
    11041 aaccaggtcc cattccccga cacgtgcgcg tgcaccgagc agttcacatt cgacaacggc
    11101 gcgggcattt cctggccgtt caacgtggcg cccggacagt ccgcgacgtt ctcgcacgag
    11161 accttcttct cgccagttgg cggatcgccg acgagcggca tcccgaattc gttccgcgac
    11221 tccgtgccga cccagatcac gctcgatccc atcgtcgtcg cccagagcat cgcgatcacc
    11281 gccggcgtga tcgtgctcgt gccgttccca tcggccctct tcaacagcac gctcgaggac
    11341 aactacgccg aggtcatggg ctggctggcc ggcatcaagg cgcgactgaa cgggctgtgg
    11401 tcgcggttcg ttgcctgggt gcgcggccag gttgcgagtc gaagggcgcc agccggcggc
    11461 tcacccgggc cggatgcggt tgctgcgtcg gccccggatg cggctggttt gtcggccccc
    11521 gatgcggctg gtttgtccag cgccgctcct gctccggcca ccgatccatg gcggtcgccc
    11581 ctgcgcatcg cgggcttcgt cctcctgacc gcgctcctgt atgcgttcct cgatccgacc
    11641 ttcggtttca gcctcgattc gctggctacg ttcaccggcc tcgcgatggg cctgttcgtg
    11701 gtgctgctcg cctacggcgt gccgctcctc atcctgtcgc ggcgcgacgg cttcacactg
    11761 accgtgcgcg cgttgccggc aacgttgttc atcggcctgc tgtgcgtgct ggtcagccgc
    11821 attgccgact tccagccggg ctacctgtac ggactgatcg tcggcttctt cttcgcgcac
    11881 ggggtcgagc gccggctcga aggccgggcc gaggccgtcg ccgcgggaac gtcattgctc
    11941 gcggcgctga tcgcctggat cgtgctcgcg ttcctgcgcg gctcgggcag ccagccgggc
    12001 gacctcttcc cggtcgtgct ggaggccgcg atggtcacgg ttgtcgtcgc cgggctcgag
    12061 aacgcggtct tcgcgatgct gcctttgcgc ttcatgcccg gcgcggccgt gtacgcctgg
    12121 gatcgccgcg tctgggtggc gctgttggcg ctgggcctgt tcggcttcgc ccacgtgctg
    12181 ctcaatcccg ccgagggcgc gggctacctg gccgacacga cgcgcacctc cttcttcacg
    12241 ctcatcctgc tgctggcggt gttcggcgtc tcgtcagtgc tcttctgggc gtacttccga
    12301 ttccggccgg cgcgcaaggc cgcgcccgcc gagtgagcac atgaccgacc gcgtcgagct
    12361 gcagctggag gtggacgccg cgcgcaccgc cgtgtacgag ttgtttgcca cagaagccgg
    12421 cctgcggcgc tggcttgacg cggccgagct cgagccgcgc gtcggcggcg cgctgagggt
    12481 caggctgcgc gacgcgactg caagcggcaa ggtgctggca ctcgacccgc cgcagcacgc
    12541 ctcgttcacc tgggagtggt ccggccagcg tgacgcgccg ccgatggtcg tggcgtttga
    12601 cgcgatcgac cacggttcgc gcacccacgt gacgctcaga cacgtcggcc tgcacacgcg
    12661 cgccgaaagc gacctgcacc atgagctgtg gcgccactgg ctggagcgat ttgaagaggc
    12721 ggtgcgcgag ctgccgcgcg aggtcgtccc gggctagcgc tggcgggctg gccctgcgcg
    12781 gtcagcccct gtagagcgtc gcggccttct ttagctccgc cgccacctca tcacgcagta
    12841 cggccggttc gagcacttcg gcgtccgcgc cccagccgag gatccagatg cggatctcgc
    12901 gcatgccggc gatctggccg cgccacagca gcgagccatc gtcttgcggc tcgagcacct
    12961 gcgaggggtg ccagcgcgtc tcggcggcgc gcttggcgac agcgggcgag aagcggatga
    13021 cgaccttgac cggatcctca tcggcgatca cgtcccaggc tttgagcagc tcgtgcgcgg
    13081 gcgactctga ctccggtggg gtgaatgtct caggggtgag cgatgccgac aggatgcgct
    13141 cgaccttgta agtgcggcgc gccttgcggc tctcgtcgta gccgatgagg tacagcgcgt
    13201 gggtgagcgc cgacggctcg atggcgagcg gccggacacg cgtcttgcgc ggtcccttct
    13261 tggggtcgta gacgcccgag tcgtactcga tctggaccac gcgccgcttg gcccacgcct
    13321 ccgccagcac ccggaacacg cgcgtgaacc ggtcgttggg cggcgttgtc gaaaaggcgt
    13381 cgacggtggc ctggatgtgc tcggccagta ccggcggcag gatcgaggcg agtttgacga
    13441 atgcgccgat cagctccgtg tcgtgctcgt cgcttgcctt ggccagcatg cgcgcggcca
    13501 ggaacagcgt cgtcgcctcg tgcatagtca gcgcgagcgg ctgcagaaaa gctcccttat
    13561 cgagcccgaa tttgccctcg tcgttccaga tcggcaggcc ggcctgctcg tccatcgcga
    13621 gcacgtcgcg gtagacggtg cgctttgaca cgccgatccg gtcagccaac gcctgcgcag
    13681 agatgccgtc ggggtagtcc ttcaggatca tcgcgatctt gaggtaccgc gcggcacggt
    13741 cccacttgcc gaagtgctcg ttggcgacgc tcacgccgtc acggtagccc gtctcgcgcc
    13801 ggcccgcctc aacagttgat caggctgctg aaaacgccgg ctgtggctcc atcggggtgg
    13861 gctcggtggg ctggaaatcg gatgccaggg ccaggccttc ggccgccgtc gcgatgcggt
    13921 tgcggccgga gcgcttggcg gcgtagcagg cccggtcggc agcgaggatg atgcttgcgg
    13981 cgtcacgcgc gtcgcgcggg aaggcggcaa ctccgaccga gcaggtcacg cccaggtcgt
    14041 tctggccctc gattgccgcg atcgcctcgt tcaccttggc cgcgaccgtg cgcgcgccgg
    14101 gcaggccggt gccgggcagc aggagcgcga actcgtcgcc gccgaaacgg aagacgaggt
    14161 cgctgtcgcg gcacgaggcg cgcagccggt cggccactcg ctggagcatg acgttgcccg
    14221 cctggtggcc gttctggtcg ttgtattcct tgaagcggtc gagatcgacc atcagcatgc
    14281 cgaagcgcgc gccgcgctcc acgaggcgtt ccaggtgctc cgtcagcgcg ccgtggttcc
    14341 acaggccggt caggcgatcg gtctcggccc gcagctcaac ggcccggtgc tgggcggcgt
    14401 tgcgcagcgc gatcgacacg tgcgcggcga agagcttgac gagctcgaac tcctcgttgg
    14461 tgaaagctgc ctcgccgccc aggcgctcca gcgccagcac gccgtggacg cgctcgccgt
    14521 tgcgcagcgg cgccacgatc agggcgcccg gccgcggctc gaggccgtac ggccgggcaa
    14581 ggcgtggctc ggcgagctca tcggacacga ggaccgcctc cccgttgcgc agcacattgc
    14641 cgctcacgct gtcgtcggtg ggcagcgaca gctggcgcac cttctccgcg tgaacaccca
    14701 ccgcgaagat ggtgttgagc cggtcggtcg tggcgtcgtg aagcatcacg ctgaggttgt
    14761 ccacctcgac cagggtgttg agccgttcgg cgatctcctc gagcagcgcc tgggtgtcga
    14821 gggtgccgag gatcgattcg gtcacgcgca ggagctcacg ctggctgctc acctggcggg
    14881 ccagcgtctc tgactgggcg tgcagctgtt cggtcgcgtc cgcgttggcc atggcctggg
    14941 cggcaatgga tgcgtagatc tcgagcagtc gcaggtcgtc ggccgtgaac tggttgagcc
    15001 ccagcttggc cagcacgatc acgcccagga cgttgtcctc gtagagcatc ggcgccagca
    15061 ggagcgactc gtcgaggtcg tcttccgtac cgggaatcgt cagcgcgcgc ttgtcgtggg
    15121 cagcgtcacc gacgttctgg gcgaggccga aacgcgccac ccagccggtc atgccctcac
    15181 cgaccttgag gcggagctgc tcgctgtcct cggagtcgta ctcgccgatc tggccgcgcc
    15241 acgccaccgg ctcgcagtcc ccgccgatga cgcgatacac acgggtgttg tgggtatcga
    15301 tcagctgtac gagctcagca cagatggcct ggccgacctc ggcgacgctg cgcagccggg
    15361 tcagccgcgc accgagctgc tggatggact gcagctgcgc tgtccatttc gccatctggc
    15421 tgtagtcctg ggcgctgcgc atcaccgcgg cgccatggcg cgcgaggcgc tcgagcagcg
    15481 cgaggtcggc ctcgctccac tcgtggcgct catcgtggta gaggcacagc acgccgaacg
    15541 ccttgtcctc ggtgatgagg ggagccacgg cgatggtgtt gaagccctcg cgccggatgg
    15601 ccgtgcgcag ctcgaagtag cgcgggtcat cgctggcgtc ggccgccgtc acggcacgtc
    15661 gttcgaccag cgacagcgtg gtcaaaggcc gggtggtggc gtgctcgagg atctggcagt
    15721 actcggctga caggttgcgc gtgaccttcg ccttgaatga gccatcggct ccagtgagga
    15781 acacgccggc gtgttgggcc ccgaacgcgt gaacggcctt ctcaaccagc tgcgcgagca
    15841 cccggtcagg gtcgcggcgg gctcccaggt cgtcatcgat ggagaacagc agctcggcgc
    15901 gatggtggcg tcgatccagg cgttcgatct gccccgagag tgcgacggcg acgtcaagct
    15961 gcgcggccac tgcggccagg agctgcggct cttcgtgatc gcgcgtcgtt gcggtgcctg
    16021 cgaggagcac gacgccaccg ccggcctggc cgtcaccaaa cggcagcgcg gcctggtagc
    16081 ggccgtcgcg ccgtcggggg gtcgtcatca ggacggcatg gcgctccgcc gcacgggcgg
    16141 ccagtcgctc gtcaggttca atcgcgccga atacgaggcg cgaggagctg tccggcgcga
    16201 ccagcgcggc gccggtgtag cccttctcct tgcacagcat ctccaccacg gattcggccg
    16261 cggagcgcgc gtccgtcttg cgcaccgcga gggcggcgga gcgcgtcagc aggccgttcg
    16321 ttgggttagc ggtctgggtt ggcgcaatcc cctcggtttg cgcgaggaag cgggcgatct
    16381 cctggcgcgc gtagcgccgg tcgccgcggc tgtttatgcg caggcacgcc aggacgccct
    16441 ggtcggtcca gctgcgcacg gtgttggcgt gaacacccag catccgcgcg gcatcgctga
    16501 cggtcaggag ctgtgtgttc gatgtggcgg tcgtggcggt ctccattcga tggaggaaat
    16561 gtcaagaagt gcgatgacga atgactcgtg ccgagcctac ccagccgcac ctgacccgcc
    16621 attggcacaa aggtcccacc tgccgcgtga ctgtcgtgcc gggttggcga accgtgtgga
    16681 taggggtgct cagcgcctct tggcaggccc cgacgttgat ctggcgccag cgaggcgtac
    16741 gtaggcgagc gtgaccaggg ccgcgagaag gccggctccg acgaccacca ggaaggccag
    16801 cccgggcgct cctacgagcg gcgcgccacc cccggcgcgt gggtcggttg gcacgccgat
    16861 gaccatgctc ggctcgggcg agggcagcgg cgaggggctg gcggccagca gagaggggct
    16921 ggcggcgagc accggttcgg cgagcgcggt tgccaggaag gccacgccca cggcaattgc
    16981 gacaagcgcg agaccggtcg cgatcagcac ggccgcggcg gctacccggc gtcgcgcgcc
    17041 gctcgcgagc aggcgcatcg cagccggccg atcgagcgcg gtcacgatca gcggccggcc
    17101 aaggccggcg ctcagcaccg gctcgccgtc gagtaactgg ggtgtgccac aggcgctgac
    17161 gtgttcgact gccgacagct gctcgatggt gagccggacg ggcgaatcag gggcgacacc
    17221 gctcgccgcg gccgccatgt cgccgtgcag ctcgctggct cggccggtgg caacccgcgg
    17281 gatggccacg aggccctcgc ccagcgcgtc ctgatcgatg gccacgaatg ccgagcgcga
    17341 ctccaccccg aacggcaccg cttcgcgctc gtcgagcacg gttcgccagc ggtctggtcc
    17401 gacctgcacc tcgaggcggg ttcggcggaa cacgagcggc cggtcattgt catcggggaa
    17461 ttcctcggtg ctgctgatcc ggccgctcac gcgcacgtag acggccgccc cggcggccag
    17521 ttcccggcac tcctcgagcg tcacctcggg cgcggccgaa agcagccggc ccaggcggaa
    17581 acgcgttccc agcgagcgca gtaggaggag cgccgcgacg agcgcaaacg ccccgccccc
    17641 ggccaggacg atcgaccagg gcacggctca gcgccacccg cgccgggcaa cgaaaaaccc
    17701 ggagaaacct tcacgaggcg tggaccttga tgtaatcgcg gtcgctagcg tcgctgctga
    17761 cccctccggg ctgcgccaac cgtatgcatg gaatccgcgc gtggtcaatc ctgttttcca
    17821 ccgattcggt cggacgcacc cgcacgtatc caccagatat ccacaggatc gtcgcctatc
    17881 tgcaccactt tgcacatcac gccgcacggc cgcttgcggc atccaggtag acatgcgccc
    17941 cgccgtacgt ggtcaggtcg agcctgcccg cgatgagcgc cgcttcaacg atcacatcac
    18001 tgtgcgccgc ccgccatgcg acctcgcctt cgcggcgcag ggcgatcgtt tcgatctccg
    18061 tctggagaag gacgtgttcg agcctcgacg acgcgataac ggccacgacc ggcgagccgc
    18121 tccggtgtgt ccaggccagc tcgccggtcc ggggctcgag cgcgtacaga tggaagccgt
    18181 agcccaccag cagcaggccg gcctcgtccc acagcgcgcc gcgcggctcg tcgtagatgg
    18241 gcgacgcgaa gcgtgccgtc cagccaccgc ctggcgactc gacatgaagc tccagccgac
    18301 acaggcggtc cgggtcgggc tcgaccagcg gcccgtgatc ggtcagggtc ccaaccgcct
    18361 ccgatatcgc aaagatcgca ggcccgccgc cgctccccgt ccagcgataa cgcgcggcga
    18421 tcccgcccgg gtattggacc gaaagccgct cgggctccgt gcgcggcagg ctcatctgcc
    18481 tgcggtcacg ccgtgccggc gttcgccgac agcggctccg tgccgggcgc ccgcccggcg
    18541 ctgatcgggg tgacgatggc attggccagg acgcgcagct cgttcggcgt taccgcaaag
    18601 acggcattcg gagtgccggc cgcggcccac acgagctcga agcgtcccag gtcgggatcc
    18661 ataagggtcc ggatgggcgc gatgtggccg aatggcggaa tgccgccgat gctgaagccg
    18721 gtcagctcgc gggcctcgtc ggcggttgcg cgccgaatat tgggctcggt cagcgccgac
    18781 gcgagcaggc cgacgtcaac cgtgttggcg ccgctcgcca ggacgaggca cgcgtgaacg
    18841 ccatctgtgc catcggccac gaagacgagg ctcttcacga tctggccgac ctccgccccg
    18901 accgcggcgg cggcctccgc ggccgtatga gtcgaacggt cgaactcctt gacctggagc
    18961 tggacaccct tgcgcgcggc agcgtcgagc acacgctgga ccgcgggttg tacgggggac
    19021 atgcggccga gtctagcggg cgccgcctca ggcgtagaat tgcagttcaa tcaaacacgg
    19081 gacaggggag cgccgccgag gcgctgagag tgcggaccag ccgcagaccc taggaacctg
    19141 atctggttca taccagcgaa gggagtccac gacgagcccc cggtccctca tccgctggag
    19201 gttcgcgcgt ggtggttgcc ctgatcgtcg ccgatggtga cgtgcctgcc cggtcagacg
    19261 ttgagcgcct gctcggcggt gtgccgcagt tggtgatcgc cgctgacggc ggggcactga
    19321 aggcccaggc gctgggctat cccgcgcggg tcgtggtggg tgacgctgat tccctcgcgc
    19381 cggcacacgt cgagcagctg cgggccgccc gcgcggaggt ggtgatccat cccgcggcca
    19441 aggatgagag cgacaccgag ctcgcgctgg aagaggcgct cagccgcggg gcgacgcgtg
    19501 tcgtgatcat cggcgcgttc ggtgggaagc ggctcgacca caccctcgcc aacatcctgc
    19561 tgctcgcctc ggagcgcctg gccggccgcg acatctgcct ggccgacggc gcgagcacgc
    19621 tgcgcgtcat gcacgacgcc gagcgcctgg cgatcacggg cgaacagggt gactacgtct
    19681 cgctgctgcc gctcaccgcg cacgtgtcgg gcgtcagcac cgctggcctg cgctatgccc
    19741 tcgccgacga gacgctccac cagggaccgg cgcgtggcct ctccaacgag ctgacagacg
    19801 ccggcgcaac gatccagatc gacgagggcc ggctggccgt gatccacacc cgcagatcag
    19861 aggtttccga ctgatgaacg aatcgaaccg gccagtgctc ctgaccatcg cggctctggc
    19921 ggtggtcgtc gtcatcgtcg ttgccgcgct gtccggcgta ttcacgaccc ccaatcccga
    19981 cgcggccggc actccaacac cgcctgtctt cacggcgcct ccgacacagc tgaccctgct
    20041 gacccatgac tcgttcgcag tgacacccga gatcctggcc gcgttcgagt cggcgaacaa
    20101 cgcgcgtgtc aacgtgctcc gcgcgggcga tgccggacag atggtcaacc aggccatcct
    20161 gacccgcgac gacccgctcg ccgacctcct ctatggcgtc gacaacacct tcctctcgcg
    20221 cgccctcgac gccgacatct tcgagccgta ccgcgccgct ggcgtcacgg cggtaccggc
    20281 ggtgctcagg ctcgacgcgc gcgagcgggt cacgccgatc gattacggcg atgtgtgcat
    20341 caacttcgac aaggaggcct tcgacgacgc caatcccgcg ccgcggaccc tggcggacct
    20401 ggccaagccg caatacagcg gccagctcgt ggtcgaagat cccgggacct catcgcccgg
    20461 cctggcattc atgctcgcga cgatcgccga gtttggtgag tcgggcgtct acacctggct
    20521 cgactactgg gccgacctac gcgacagcga cgtggccatc gtgtccggct ggtcggaggc
    20581 ctacgagagc actttctcgg gcgggtctgg ccggggcgat cggccgcttg tcgtctcgta
    20641 cgcgacgagc ccggtggcgg aggttttcta tgcgagtccg cagccggcag aggcgccaac
    20701 aggcgtgatg accgacggct gcttccgcca ggtcgagttc gccggtgtcc ttgccgacac
    20761 agcgcagcct gagctggcca ggaagttcat cgacttcatg ctgaccgccg aattccaggc
    20821 cgacatcccg ctcaacatgt tcgtcttccc ggcggtaccc gcgacgaata tcgcccaggt
    20881 cttcagggat tacgcggtgg tggtgccgga gccactgacc atggcgcccg aacagatcgg
    20941 cctcaatcgc gagcgctggt tgaacgagtg gcaagccgca acgcactgac gctcgcgctg
    21001 gcggcgctgc cgctggcgtt cgtcgggctg ttcttcgtct ttccggtcgc gtcgatcctg
    21061 gtgcgcggcc tcgccccgaa cggcgtgctt gacctggctg tgccgctgca ggtgctgacg
    21121 c
//
LOCUS       DATBRX010000197        12977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3541672, whole genome shotgun sequence.
ACCESSION   DATBRX010000197 DATBRX010000000
VERSION     DATBRX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3541672"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VIF08_07675"
     CDS             complement(<1..534)
                     /locus_tag="VIF08_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8135907.1"
                     /translation="MSSADNPAVAVNQMSADRPRAPALSFFFPAHNEAENIEALVAEA
                     LQVLPRLADEFEIIAVDDGSKDATPRLADELAARHPQVRAVHHEVNRGYGGALRSGFR
                     AARYELVCFLDGDRQFHVEDLGRLLERQAAADNPDVVVGYRIKRADPFVRLAYARAYR
                     LALRIFYGLKVRDPDCAC"
     gene            complement(531..2060)
                     /locus_tag="VIF08_07680"
     CDS             complement(531..2060)
                     /locus_tag="VIF08_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135908.1"
                     /translation="MHPSIATTYPPAGQESAGGNDLAGRLRTLLERADPAHIGLFFVI
                     VALGIYIFSDPARSGWYNHFVWQSDAFLHGRIAIAYPVSDGPYVNSYFQDIMPLPSAP
                     GEPSYGLLPFPPLPAVLLMPFVAIFGLATDAQLFGAVLGAINVGLAWRLTTRLTESRS
                     AAFLATLFFAFGTVHWYAAIISTTWFLAHVVAVTFLLLGVTLALDAERREQLRRVMRI
                     RAADESVHAARNPLAETFEQASLFVAWGRRLFGQIDALQFMAGFVFGLAALSRLTIIF
                     GAPFFLFVGGGGSYFRRALSAGIGAAIPVALIGIYNYLSTGHLFNPVYEFLFQQQFHG
                     LPYAPSEEFYGQREWGPENPLYIPLNAVLMFLWPPDLSPTYPPFDQCANVARTVLGAC
                     PVAVPSKFGMSIFLTSPAFLLIPFVAAVAWRQRVVLGALLAVVAIGLVNLGHFSQGWV
                     QFGYRFSLDFAPFSLVLVTIGIAWLARSPRGLAWVAVPLVIMSVLVNAWGVYWGVKLG
                     W"
     gene            2090..7630
                     /locus_tag="VIF08_07685"
     CDS             2090..7630
                     /locus_tag="VIF08_07685"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEY8135909.1"
                     /translation="MTSPRAGGADRFLGARRARGTRTAVGSLAILLLVGLLLRLTIAY
                     VLLPGSGFESDIGTFTAWAIQLGQHGPGTFYASAGFADYPPGYLYVLWLLGGLGGLLA
                     PFANGDAVSATAALIKIPPMLLDIAVGAALFYVVRSWRANRPDASRLGLIAAALYVLN
                     PVTWYDSAVWGQTDAAGALIILLTVFALVRGNSEGATVLAVLAALIKPQFGVVALPIV
                     GIVLLRRHLFRPGTNPRNPVLVPERFRTWFEVEDGFWRLVSSAVAGLVVLLLMLAPFS
                     MDVVRFFNQMTGTAGGYPWLSVNAYNPWALMGAGGSAPLAFGGGWSSDTVGLLGPLPG
                     VLIGGILLALGFAVGVLRLAWQDDRRSLVTVSIFLALAFFMLPTRVHERYMFPIFGLL
                     PLLAVVDRRWMTATIALSIAAFINLHGILTTDLYATPNLEHLPLGELFRQPLGILIAI
                     FLNVAGFVLIALRMRPSEAARPAPPPVRADARDPAPVGPPGPRAAQAPWSTQPTRFGD
                     GQTAPEATGAAVTSAAVQRSQGEPELDDEPLTDDDGEPAEPAPWQRWISSVIDRPSVR
                     RDRSKLLINERGGRLDRRDLALVLLVFAASLFLRTYRLEVPYGPHFDEVYHARTAVEF
                     LQDWRYDMPHSIYEFTHPHLAKYAMAWGITLLGNNRVTDTRDLGTPVRDADTETRWSP
                     PDQPGLRNGDRLYVVTGSELRVYDLATKQQVATLPGPFVGAAIDQTAHMLYLADAAGS
                     ISTMPTDQLDAARRAGADPLLPTAYPFADATDLVGPISRIYATGTELLAVSDGGSVVS
                     FDLAGLETGRATFSNGVTGAGVPPRAEVLVDSSQVSDFGAYAHTLAGLISNDASRIQG
                     VLDTANGQQVVVAGDVGQFHADVQEAIDAGDLPGTTIVGVPPRAEILVDPTQVTDFAD
                     YAHTLAGLISDDAARIETLVSTSTDPRTILAGDIAPMVDDVQAAIDDGRLPGTTIEER
                     PMVAVGTSTGIVLLDAASLVEIKRFDSSHPVGGMALVSRGPDVPTIYAASGAQLITLR
                     LPTDGEAALGSPLTMPNTVQDVRWNEATTNIHVLGRAQDATTPTVYVIEPRSNAIFAD
                     APLSFEPRALTLDVQRDRPAEDRDDLLALSEDGVLATIDVGNNGWAYRFPGVLVGALM
                     AVCIYLLARFLSARRAVALIVALLVLADGMFFANARIAMNDTYVAFFIVAAMTLFVPL
                     WLGRWRNPFALIVGLPAVGVLLGLAFASKWVGLYAIGAVGLLILIRSALGRWIALVAM
                     VALTAVLGYIAINPATNVANPSLNYLFLFVMVGLTVVLALAMALRPMRFSADELRMGA
                     LAVLVPGPLILLYSLYKSSQGGLPPLGTFLTPARLLQLGAGVTFVGLAIVAGLWFAGR
                     RGYGPLARRIVDQDETPAEPPAPRGWLRPGSGFLGLPWLLALGALLIIPLAVYMASYI
                     PWINLGNHWGMPSWLPFFPQGTEGQAFLDLQKSMYNYHDTLRATHPASSPWWAWLLDL
                     KPVWFEQSNYANGTTAVIYDTGNLVAFWLAIPAIGWTSWQAWKRRSLPLTFVAVAIAC
                     LWLPWARIDRATFQYHIFTTLPFTFLALAYFLAELWHGPSRGTWAVARVGAALAVIGA
                     PLLWLLRQPLCGLANTQSVNPNTEVCAALSRTLTLTDIQVVGLGLAIVGLVAAGTIVY
                     VSMSSGPDVTRPSYWPLLLPVSFSVALFGVVIAVIGAALTGKPVFSADVRAEEPALVA
                     LLLLAVPAYFVLRASDPRKFVVGALTAAVIWFVAFYPNIASLPVPTPLSQIHLGLLPT
                     WNWGFQFGVNRDPAVNMPISWPSVALLAVAVIGLCIAAVYAARTWRSARYSDDEVSEL
                     PETS"
     gene            complement(7636..8586)
                     /locus_tag="VIF08_07690"
     CDS             complement(7636..8586)
                     /locus_tag="VIF08_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135910.1"
                     /translation="MNSTSQDGLAPLELPDSIRRASLTLKVLAVIYVLGIIFAGLPGI
                     VPTSLFETVAFNLFAAGLAVVFGVVALALDRKQPWALSTVRLLLALLVIYGAYTFVSL
                     LLAGKFRIPTTMLVAGFGLFMPADEWPAMKPSVRGGGVLVLVAALLGLQTASPALFGW
                     GGYFDVHQGDLDAALVVDCGTAGPPEQLAITYEWSWSRDTLLANDEDQVVIGWEGDGV
                     DGRPMYVAIDLPAQSDGIYLGVSSGASGPMASQVAATWRGVFMVRLDLHKLEVRPGRI
                     AAVLVRTAAQPAPEQSVTFGVSYIHSGVWRNDVETVTCTW"
     gene            complement(8608..9801)
                     /locus_tag="VIF08_07695"
     CDS             complement(8608..9801)
                     /locus_tag="VIF08_07695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8135911.1"
                     /translation="MKIGLVTPYIYPLPGGVNAHVKFLYENLIARGHDVRIISSVHGA
                     QRTDEGDVIRLGYGFSVPANGSVGTMTVSPRLAHQVREMLERERFDVLHFHEPFVPFL
                     SLILLRESQSINVATFHAYAGWSPAYEFGKRMLGRFARRLHGRIAVSAAARHFIDRYF
                     PGDYKVIPNGVDIARLEAAQPIARYRDGTLNILFLGRFESRKGALYLLKAYRVLRRRG
                     INCRLLLIGAGPQEREIRRYVATRRLTGVELLGRVSDDDKARYLATADVFVSPATGGE
                     SFGIVLLEAMAAGTPIVCSDIHGYKGVVRRDQQALLVPPRDVKALTEALARVLGDADL
                     RARMGAAGRERAPQFSWQNITAKVEDYYLFVARRAASHGPLPAHVNPRIIGEPISITQ
                     LRAAR"
     gene            complement(9804..10604)
                     /locus_tag="VIF08_07700"
     CDS             complement(9804..10604)
                     /locus_tag="VIF08_07700"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEY8135912.1"
                     /translation="MTNASADQHEGGRGRADLHIHSLASDGISSVGEILAAAEAAKLD
                     VIAITDHERMDAALAAHALAEASGSKVSVIVGEEVTTRSGHVIGLFMSERIRPWGSLR
                     STIARIHEQGGLAIIPHPLVPYPLCVSGRAVRALLDDPNPMFHPDAIEAFNASTARMR
                     WSRGAPEFARRIGLTALAGSDAHRAADVGHTVTTFPGRTPDDLRAAIAAGTTEWSGDA
                     YTWRSQLDMFVRQQHKNARAVGATARHRLLGAGLGRDLGYPRTSAERG"
     gene            complement(10601..11602)
                     /locus_tag="VIF08_07705"
     CDS             complement(10601..11602)
                     /locus_tag="VIF08_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8135913.1"
                     /translation="MTTDANVQATPRRDSPRATQPRVTIRTRLLILAATVLQHLPDGL
                     LQRIAHALGGVMYRVRPARRRLVRANLQRVVTWLVAHDMANPQVRAAATDPRALDRLV
                     RAAFGHYLRGYLESATLPRYGTAERLKRVQADDWSAADRAFKNGPGGKAGRLIIVSLH
                     FGAIEIPGLWATKTLGGRITAPMETIGDPGLQAYFERARRGTGMNVIPVEGAAAELRA
                     ALERDEIVALVADRAVGGVGSTVELFGAPARLPAGPALLSDETGAPAWLIVTRRVGWG
                     DYRSRMEQIVPGAPAGDSRRDRLSAFLDAEARAFERAIADAPEQWWTVFFQIWPDIK"
     gene            complement(11599..12621)
                     /locus_tag="VIF08_07710"
     CDS             complement(11599..12621)
                     /locus_tag="VIF08_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8135914.1"
                     /translation="MNQPKGGVAYAPHRREARSSRIGQAVRRARERSAVRGYRAAAAA
                     IGRIPARLSLPVGKALLVGSYFAWPTKRRIILANASHVLQLPASDRRVRHLARRIYAT
                     YAQFIIELMRLPRLPASEPARLVRGAANQGDESFFALFERYRAEGRGMIVVSAHIGSI
                     DMMAGSMAYRGLPCYGVADDSAYPELFEAINEQRRRWGIQVIPWKNLRRTFKALREGG
                     IVGLVVDWGYRAEDVPVKLFGEWATLPAGPVMLAARTNAPIVPAVCRRMPDGFYEARH
                     YDPIEVANDSPAETLRVTRLIATAVEDMIATAPEQWYSFKPIWPDTEAEKQKLADRAA
                     QMESGA"
     gene            complement(12632..>12977)
                     /locus_tag="VIF08_07715"
     CDS             complement(12632..>12977)
                     /locus_tag="VIF08_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135915.1"
                     /translation="LEVWDSPNSAGVITDAIRCIKLGLDRGLKGTLVAPSSYFMKSPP
                     RQIHDDIAFNRVEAFIRGEDNETLVGTEKAPAPKRRRLGAHDRVATKSQTSANAKAAA
                     TAAAAKVEAKAR"
BASE COUNT         1969 a         4486 c         4455 g         2067 t
ORIGIN      
        1 gcacgcgcag tcggggtcgc gcaccttgag gccgtagaag atacgcagcg ccaaccggta
       61 ggcgcgcgcg taggccagcc gaacgaacgg atcagcacgc ttgatcctgt aaccgaccac
      121 cacgtctggg ttgtcggccg ctgcctggcg ctcgagcagc cgacccaggt cctcaacgtg
      181 gaactggcgg tcaccgtcga ggaagcagac cagctcatag cgcgccgccc ggaagccgct
      241 gcgcagcgcc ccgccgtagc cgcgattgac ctcgtggtgc acggcacgca cttgggggtg
      301 gcgcgcggct agctcgtcgg ccagacgtgg cgtggcgtcc ttgctgccgt cgtcgacagc
      361 gatgatctcg aactcgtcgg ccaagcgcgg caggacctgg agcgcctcgg caacgagcgc
      421 ctcgatgttc tcggcctcgt tgtgcgccgg gaagaagaac gacagcgctg gcgcgcgtgg
      481 ccggtcggcg gacatctggt tcacggccac ggcgggatta tctgcgctgc tcaccagccg
      541 agcttcacgc cccagtacac gccccacgcg ttgaccagga ccgacatgat caccagcggc
      601 accgcgaccc aggccaagcc gcgcggcgac ctggcgagcc acgcgatgcc gatcgtcacg
      661 agcacgagcg agaacggcgc gaagtcaaga ctgaagcgat agccgaattg gacccagccc
      721 tggctgaagt ggcccaggtt gacgaggccg atcgcgacga cggccagcaa cgcgcccagg
      781 accacgcgct gccgccaggc gacggcggcg acgaatggaa tgagcaggaa ggccgggctg
      841 gtcaggaaga tgctcatgcc gaacttgctg ggcaccgcga ccgggcacgc tcccaaaacg
      901 gtgcgagcga cattcgcgca ttgatcaaaa ggtgggtaag tgggcgagag atcgggcggc
      961 cagaggaaca tgagcacggc gttcaacggg atgtagagcg ggttctccgg gccccactcg
     1021 cgctgtccat agaactcttc cgacggcgcg taggggagcc cgtgaaactg ctgctggaag
     1081 aggaactcat acaccgggtt gaacaggtga cccgtgctca ggtagttgta gatgccgatc
     1141 agcgccactg gtatcgccgc gccgatgccc gccgatagtg cgcgcctgaa gtacgagcca
     1201 ccgccgccga cgaacaggaa gaagggcgcg ccaaagatga tcgtgagccg tgacagtgca
     1261 gcgagtccga agacgaaacc ggccatgaac tgcagtgcgt cgatctggcc gaacagccgt
     1321 cgcccccaag cgacaaagag gctcgcctgc tcgaacgtct cggcgagcgg gttgcgcgca
     1381 gcgtgaactg attcatcggc cgcgcgaatg cgcattaccc ggcgcagctg ctcgcgccgc
     1441 tcggcgtcga gcgccagcgt gacacccagc aggaggaagg tgaccgcaac aacgtgcgcc
     1501 aggaaccacg tcgtactgat gatggcggcg taccagtgaa ccgtgccgaa ggcgaagaag
     1561 agcgtcgcca ggaaggcggc cgagcgcgac tccgtcaggc gcgtcgtcag gcgccacgcc
     1621 agacccacgt tgatggcgcc caacaccgcg ccaaacagct gcgcgtcggt agccaggcca
     1681 aagatggcca cgaacggcat gagcagcaca gcgggcagcg gtgggaacgg cagcaggccg
     1741 tacgacggct cccccggggc cgatggcagc ggcatgatgt cctggaagta gctgttgacg
     1801 tagggaccgt cggagaccgg gtaggcgatg gcgatgcggc cgtggaggaa cgcgtcggac
     1861 tgccagacga aatggttgta ccagccgctc cgcgccggat cggaaaagat gtagatcccg
     1921 agcgccacga tgacgaagaa gaggccgata tgggccggat cggcacgctc caggagggtg
     1981 cgcaggcgcc ccgcaaggtc attcccgcca gccgactcct ggccggccgg cgggtaggtc
     2041 gtcgctatac tcgggtgcac ttggtcatcc cctggaggtt cggtcgagag tgacatcgcc
     2101 ccgggcaggc ggggccgacc gattcctcgg cgcgaggcgt gcgcggggga cgcgtacggc
     2161 cgtcggcagc ctcgccatcc tgcttctcgt cggtctactc cttcgcctaa ctattgcata
     2221 cgtcctgctg ccaggctcgg gcttcgaatc ggacatcggg acgttcacgg cgtgggcgat
     2281 tcaactgggt cagcacggcc cgggcacctt ctacgccagc gcaggcttcg ccgactaccc
     2341 gccggggtac ctatacgtcc tgtggctttt aggggggtta ggtggtctgc tcgcaccgtt
     2401 cgccaacggc gatgctgtgt cggccacggc ggcgctgatc aagatcccgc cgatgctgct
     2461 cgacatcgcg gtgggcgcgg cgctgttcta cgtcgtgcgc agctggcgcg ccaaccggcc
     2521 cgacgcctcg cgcctggggc tcatcgcggc cgcgctctac gtgctcaacc cggtcacctg
     2581 gtacgactct gccgtctggg gccagaccga tgccgccggc gcgctcatca tcctgctcac
     2641 ggtgttcgcg ctggtgcgcg gcaactccga aggcgcgacc gtgctggccg tgctggcggc
     2701 gctgatcaag ccgcagttcg gcgtggtggc gctgcccatc gtgggcatag tcctgttgcg
     2761 ccgccacctg ttccggccgg gcacgaaccc gcgcaaccca gtgctggtgc ccgagcggtt
     2821 ccgcacctgg ttcgaggtgg aggacgggtt ctggcggctc gtctcatctg ccgtcgccgg
     2881 actggtcgtc ctgttgctga tgctcgcgcc cttctcgatg gacgtggtgc gcttcttcaa
     2941 ccagatgacg ggcacggccg gcggctaccc gtggctgagc gtcaacgcct acaacccctg
     3001 ggcgctcatg ggcgccggtg gctccgcccc gttggccttt ggcggtggct ggtcgtcaga
     3061 taccgtgggc ctgctcggcc cgctgccggg cgtgctgatc ggcggcatcc tgctcgccct
     3121 gggcttcgcg gtcggcgtcc tgcgcctggc gtggcaggac gaccggcgca gcctcgtcac
     3181 ggtgtcgatc ttcctcgcgc tggcgttctt catgctgccg acgcgcgtcc acgagcgcta
     3241 catgttcccg atcttcgggc tgctgccgct gctcgccgtg gtcgaccggc gctggatgac
     3301 ggcgacgatc gcgctgtcaa tagcagcgtt catcaacctg cacggcatcc tgacgaccga
     3361 cctctacgcc acacccaacc tcgagcacct gcctttaggc gagctgttcc gccagccact
     3421 cggcatcctc attgcgatct tcctcaacgt ggctggattc gtgctgattg cgcttcgtat
     3481 gcggcccagc gaggccgcgc gacccgcgcc gccacctgtt cgagctgacg cgcgcgatcc
     3541 cgcgccggtc gggccgcccg ggccgcgggc cgcccaggca ccgtggagca cccagccaac
     3601 gaggttcggc gacggccaga cggcaccgga ggccactggg gccgcggtca cgagcgcagc
     3661 cgtgcagcgg agccagggcg agcccgagct cgacgacgaa cccctcacgg atgatgacgg
     3721 cgagccggcc gagccagcac cctggcagcg ctggatcagc tccgttatcg atcgcccgtc
     3781 ggtgcggcgc gaccgcagca agctcctgat caacgagcgc ggcggccggc tggaccggcg
     3841 cgacctggcg ctcgtgctgc tggtcttcgc cgcctcgctg ttcctgcgca cgtaccggct
     3901 cgaggtgccg tacgggccgc acttcgacga ggtctaccac gcgcgcaccg cggtcgagtt
     3961 cctccaggac tggcgctacg acatgccgca ctccatctac gagttcaccc acccgcacct
     4021 ggccaagtac gccatggcct ggggcatcac cctgctcggc aataaccgcg tcaccgacac
     4081 gcgcgatctg ggcacgccgg tccgtgacgc cgacacggag acacgttgga gcccgcccga
     4141 ccagcccggc ttgcgcaacg gcgatcggct gtacgtggtc accggcagcg agctgcgggt
     4201 ctatgacctt gccacgaagc agcaggtagc gacgctgccc gggcccttcg tcggcgcggc
     4261 catcgaccag accgcgcaca tgctctacct cgctgacgct gcgggctcga tctcgaccat
     4321 gccgacggac cagctcgatg ccgcgcgtcg ggcgggcgcg gatccgctgc tgccgaccgc
     4381 ctacccattt gcagacgcca ccgatctcgt cggaccgatc agccgcattt acgccaccgg
     4441 aacggagctg ctggcagtct ctgacggcgg gtccgtggtc agcttcgatc tggccggtct
     4501 cgagaccggc cgcgccacgt tcagcaatgg cgtcactggg gccggcgtgc cgccgcgcgc
     4561 cgaggtgctt gtcgattcaa gccaggtcag cgacttcggc gcttacgcgc acacgcttgc
     4621 cgggctcatt tcgaacgatg cctcacgcat ccaaggtgtg ctcgacacgg ccaatggcca
     4681 gcaagttgtc gtggccggcg acgtgggcca attccacgcc gacgtccagg aagcgatcga
     4741 cgccggcgat ttgcccggca cgaccatcgt cggcgtgccg ccgcgcgccg agatccttgt
     4801 cgatccgacg caggtcaccg acttcgcgga ctatgcccac acgcttgccg gcctgatttc
     4861 ggacgacgcg gcgcgcatcg agactctcgt atccacctca actgacccgc gcacgatcct
     4921 cgccggcgac atcgccccga tggtcgacga tgtgcaggcc gcgatcgacg acggtcgcct
     4981 gcccggcacc accatcgagg agcggcccat ggtggcggtg ggcacgagta caggaatcgt
     5041 cctgctcgat gctgcgtcgc tcgtggagat caagcgcttc gatagcagtc atccagtggg
     5101 cggtatggca ctcgtatcgc gcggtccaga tgtgccgact atctatgccg cgagcggcgc
     5161 gcagctcatc acgctgcgcc tgcccacgga cggcgaggcc gcgctcggct cgccgctcac
     5221 gatgcccaac acggtccagg acgtgcgctg gaacgaggcg acgaccaaca tccacgtcct
     5281 gggccgggcg caggacgcga cgaccccaac tgtgtacgtg atcgagccgc gctcgaatgc
     5341 gatatttgcc gacgcccctc tgtccttcga gccgcgcgcc ctgacgctgg acgtccagcg
     5401 cgaccgaccg gcggaggatc gcgatgacct cctggcgttg agcgaggacg gtgtcctcgc
     5461 gacgatcgac gtgggcaaca acggctgggc ataccgcttc ccgggcgttc tcgttggcgc
     5521 gctgatggcg gtctgcatct acctgcttgc gcgcttcctg tccgcccgcc gcgcggtggc
     5581 gctcatcgtc gcgctgctcg tcctggccga cggcatgttc ttcgccaacg cgcgcatcgc
     5641 catgaacgac acctacgtcg ccttcttcat cgtcgccgcg atgacgctct tcgtgccact
     5701 ctggctgggc cggtggcgca atccgtttgc gctgatcgtc gggctgccgg ctgtgggcgt
     5761 gctgctgggg ctcgcattcg cgagcaagtg ggtcgggctg tatgcgatag gggcagtcgg
     5821 gctgttgatc ctcatccgct cggcgctggg ccgctggatc gccctggtgg cgatggtcgc
     5881 gctgaccgcg gtcctgggct acatcgcgat caacccggcg accaatgtcg ccaatccgtc
     5941 gctcaactac ctgttcctct tcgtgatggt gggcctgacc gtggtgctgg cattggccat
     6001 ggctctgcgg ccgatgcgct tctctgccga cgagctgcgg atgggcgcct tggcggtgct
     6061 ggtgccgggg ccgctgatcc tgctctacag cctctacaag tccagccagg gcggtctgcc
     6121 gcctctgggc accttcttga cgccggcccg tctgttgcag ctcggcgcgg gcgtgacctt
     6181 cgtcgggctg gccatcgttg ctggactctg gttcgccggg cggcgcggtt acggcccgct
     6241 ggcaaggcgc atcgtcgacc aggatgaaac gccggccgag ccgcccgcgc cgcgcggctg
     6301 gctacggccc ggttcgggct tcctgggcct gccctggctg ctcgccctgg gcgcgctgct
     6361 gatcattccc ctggcggtct acatggccag ctacatcccg tggatcaacc tgggtaacca
     6421 ctggggcatg ccgtcgtggc tgccgttctt cccgcagggc accgagggcc aggcattcct
     6481 cgatctccag aagtcgatgt acaactacca cgacaccctg cgcgcgacgc atccggcgtc
     6541 gtcgccgtgg tgggcttggc tgctcgacct gaagcccgtc tggttcgagc agagcaacta
     6601 cgccaacggc acgacagcgg tcatctacga caccggcaac ctggtcgcat tctggctggc
     6661 catcccggcg atcggctgga cgtcctggca ggcctggaag cggcgcagcc tgccgctcac
     6721 ctttgtcgcg gtcgcgatcg cctgtttgtg gctgccctgg gcgcgcatcg accgcgcgac
     6781 gttccagtac cacatcttca ccacgctgcc gttcacgttc ctggcgctgg cctacttcct
     6841 ggccgagctg tggcacgggc cgtcgcgcgg cacgtgggcc gtcgcccgcg tcggcgccgc
     6901 gctcgcggtg atcggcgcgc cgctgctatg gctgctgcgc cagccgctgt gcggcctggc
     6961 caacacccag tcggtcaacc ccaacacaga agtctgcgcc gcgctctcgc gcaccctgac
     7021 cctgaccgac atccaggtcg tcggcctggg gctcgcgatc gtgggcctgg tcgcggccgg
     7081 gacgatcgtc tatgtgtcca tgagctctgg ccctgacgtg acgcgcccca gctactggcc
     7141 gctgctgctg ccggtgtcct tctccgtcgc gctgttcggc gtcgtcatcg ccgtgatcgg
     7201 tgcggcgctg actggcaaac cggtgttctc cgccgatgtg cgtgccgagg agccggcgct
     7261 ggtggcgttg ctgctgctcg ccgtgccggc gtatttcgtg ctccgggcga gcgacccgcg
     7321 caagttcgtg gtgggcgcgc tgacggccgc ggtcatctgg ttcgtcgcct tctacccgaa
     7381 catcgccagc ctgcccgtgc cgacaccgct gtcacagatc cacctcgggt tgctgccgac
     7441 ttggaactgg ggcttccagt tcggggtcaa ccgcgatccg gcagtcaaca tgcccatctc
     7501 atggccgagc gtcgcgctgc tggccgtggc cgtgatcggc ctgtgcatcg ccgcggtcta
     7561 tgccgcgcgc acctggcggt cggcgcgcta ttcggacgac gaggtcagcg aactaccgga
     7621 gacgagctga gagctctacc aggtacaggt caccgtctcg acatcgttgc gccagacgcc
     7681 ggagtggatg tagctgacac cgaatgtcac gctctgctcc ggggccggct gcgccgccgt
     7741 gcggaccagc accgccgcga tgcgacccgg tcggacctcc agcttgtgca ggtcgaggcg
     7801 caccatgaac acaccgcgcc acgtggccgc aacctggctg gccatcgggc cactcgcgcc
     7861 gctcgacacg cccaggtaga tcccgtccga ttgggcaggc aggtcgatgg ccacgtacat
     7921 gggccggcca tccacgccat cgccctccca gccgatgact acctgatcct cgtcgttggc
     7981 tagcagcgtg tccctggacc acgaccactc gtaggtgatc gcgagctgct ctggcgggcc
     8041 ggcggtgccg cagtcaacga cgagcgccgc gtccaggtcg ccctggtgca cgtcgaagta
     8101 gccgccccac ccgaacagcg ccgggctggc cgtttgcagg cccaatagcg cggcgaccag
     8161 cacgaggacc ccgccgcccc ggacgctggg cttcatggct ggccactcgt cggccggcat
     8221 gaacaggccg aacccggcaa ccagcatggt cgtcgggata cggaacttgc ccgcgaggag
     8281 gagcgacacg aacgtgtagg cgccatagat caccagcaac gcgagcagga gtcggacggt
     8341 cgacagggcc cacggctgct ttcgatcgag ggccagcgcc accaccccga acacgactgc
     8401 cagacctgcg gcgaagagat tgaacgcgac ggtctcgaag agcgacgtcg ggacgatgcc
     8461 gggcaggccc gcaaaaatga tgcccaggac gtagatgacg gccaggacct tcagcgtcag
     8521 cgacgcgcgc ctgatgctgt ccggcagctc gagcggggca agtccgtctt gcgacgtgga
     8581 gttcatggct ggcctctgcg ggccgactca gcgcgctgcg cggagctgcg tgatcgagat
     8641 cggctcgccg attatccgcg gattgacgtg cgccggcagc ggaccgtgcg acgccgcacg
     8701 acgcgccacg aaaaggtagt agtcctcgac cttggcggtg atgttctgcc agctgaactg
     8761 gggcgcgcgt tcgcggccgg cagcgcccat ccgggcgcgc aggtcggcgt cgcccaggac
     8821 gcgcgccagc gcctcagtga gcgcctttac atcgcgcggc ggcacgagca gcgcctgctg
     8881 gtcgcggcgc acgacaccct tgtagccgtg gatgtcgctg cacacgatcg gcgtaccggc
     8941 ggccatggcc tcgagcagca cgatgccaaa cgactcgcca ccggtcgcgg gtgacacgaa
     9001 gacgtccgca gtcgcgaggt agcgcgcctt gtcgtcgtcg ctgacccggc cgagcaactc
     9061 aaccccagtc aggcgccgcg tcgccacgta gcgtcggatc tcgcgctcct gcggccccgc
     9121 accgatgagc agtagccggc agttgatgcc gcgccggcgt agcacgcgat acgccttgag
     9181 caggtacagc gcgcccttgc ggctctcgaa gcggccgagg aacaggatgt tgagcgtgcc
     9241 gtcgcggtag cgcgcgatcg gctgggcggc ctcaaggcgg gcgatgtcga cgccgttggg
     9301 gatgaccttg tagtcgcccg ggaagtagcg atcgatgaag tgccgcgcgg cggcgctgac
     9361 cgcgatgcgg ccgtgcaacc ggcgcgcgaa ccggccgagc atgcgcttgc cgaactcgta
     9421 cgccgggctc cagccggcgt aggcatggaa cgtcgcgaca ttgatgctct ggctctcgcg
     9481 cagcaggatg agcgacagga agggcacgaa cggctcgtgg aaatggagca cgtcgaaacg
     9541 ctcgcgctcg agcatctcgc gcacctggtg ggcaagccgc ggcgagacgg tcatcgtccc
     9601 gacggagccg ttcgccggga cgctgaagcc atagcccagg cggatcacgt cgccctcgtc
     9661 ggtccgctgt gcgccgtgga cgctgctgat gatgcgcacg tcgtggccgc gcgcgatgag
     9721 gttctcgtac aggaacttga cgtgcgcgtt gacgccgccc ggcaaggggt agatgtacgg
     9781 cgtcactagg ccgatcttca ttgctagccc ctctcagcgc tcgtgcgcgg atagcccagg
     9841 tcgcgaccca ggcccgcgcc gagcaggcga tggcgcgcgg tagcgccgac cgcccgcgcg
     9901 ttcttgtgct gctggcgcac gaacatgtcg agctggctgc gccaggtata ggcgtcgccc
     9961 gaccattccg tggtgccggc agcgatagcc gcgcgcaggt catccggcgt gcggcccggg
    10021 aaggtggtca ccgtatggcc gacatccgcg gcgcgatggg catcgctgcc ggccagcgcg
    10081 gtcaggccaa tccggcgcgc gaactcgggc gcgccgcgtg accagcgcat ccgcgcggtc
    10141 gacgcgttga aggcctcgat cgcgtccggg tggaacatgg ggttggggtc gtccagcagc
    10201 gcgcggaccg cccggccgct gacgcacagc ggatatggca cgagcggatg agggatgatc
    10261 gccaggccgc cctgctcatg gatgcgcgcg atcgtcgagc gcagcgagcc ccatggtcgg
    10321 atgcgttcgc tcatgaacag cccgatcacg tgccctgacc gggtcgtcac ctcctcgccg
    10381 acgatcacgc tgaccttcga gccggaggcc tccgccagcg cgtgcgcggc gagtgccgcg
    10441 tccatccgct cgtgatcggt gatggcgatc acgtcgagct tcgcggcttc ggccgcggcg
    10501 aggatctccc cgaccgacga tatgccgtcc gaagcgaggc tgtggatgtg caggtcggcc
    10561 cgaccccggc ctccctcgtg ctgatcggcc gacgcgttcg tcatttgatg tctggccaga
    10621 tctggaagaa caccgtccac cactgctcgg gcgcgtcggc gatggcgcgc tcgaatgccc
    10681 gcgcctcagc gtcgaggaag gcgctcaggc ggtcgcggcg cgagtccccg gccggtgcgc
    10741 cgggcacgat ctgctccatg cgcgagcggt agtcgcccca gccaacgcgg cgggtcacga
    10801 tcagccacgc gggtgcgccg gtctcgtcgc tcagcagcgc cgggccggcc ggtagccgcg
    10861 ccggtgcacc aaagagctcg actgtgctgc cgacgccacc gaccgcgcgg tcggccacca
    10921 gggccacgat ctcatcgcgc tccagcgccg cgcgcagctc tgcggccgcg ccttcgacag
    10981 gaatcacgtt catgcccgtg ccgcgccggg cgcgctcgaa gtacgcctgc aacccgggat
    11041 cgccgatcgt ctccattggc gcggtgatcc gcccgccgag cgtcttggtg gcccacaggc
    11101 cggggatttc gatcgcgccg aaatggagcg agacgatgat cagccgcccg gccttgccac
    11161 cagggccgtt cttgaaagcc cggtcggcgg cgctccagtc gtcagcctgg acgcgcttga
    11221 gccgctccgc cgtgccgtag cgcggcagcg tggcgctctc gaggtagccg cgcaggtagt
    11281 ggccgaacgc agcccgcacc agccgatcca gcgcccgggg gtcggtcgcg gccgcgcgta
    11341 cctgcgggtt ggccatgtcg tgcgcgacga gccacgtcac gacgcgctgg aggttggcgc
    11401 gcacgagcct ccggcgcgcc ggtcggacgc gatacatgac gccgccgagc gcgtgggcga
    11461 tgcgctggag cagcccatcg ggcaggtgct ggagcacggt cgcggccagg atgagtagcc
    11521 gagtgcgaat ggttacgcgg ggctgcgtgg cgcggggtga atcgcgccgc ggcgtggcct
    11581 ggacgttagc gtcggtggtc acgcgcccga ttccatctgc gccgcccgat cggccagctt
    11641 ctgcttctcg gcctccgtgt cgggccagat gggcttgaag ctgtaccact gctccggcgc
    11701 ggtggcgatc atgtcctcga ccgcggtcgc gatcaggcgc gtgacgcgca acgtctccgc
    11761 gggcgaatcg ttggccactt cgatggggtc gtagtggcgc gcctcgtaga aaccgtcggg
    11821 catgcgccgg cagaccgcgg ggacgatggg cgcattggtc cgcgctgcga gcatcacggg
    11881 acctgccggc agcgtggccc actcgccgaa gagcttgacc ggcacgtcct ccgcgcgata
    11941 gccccagtcg acgacgagac ccacgatgcc gccctcgcgc agcgccttga aggtgcgccg
    12001 caggttcttc cagggaatga cctggatgcc ccagcggcgg cgctgctcgt tgatcgcctc
    12061 gaagagctcc gggtaggccg agtcgtcggc cacgccgtag cagggcaggc cgcgataggc
    12121 catcgagccc gccatcatgt cgatgctgcc gatgtgggcc gacacgacga tcatgccccg
    12181 gccctctgcg cggtagcgct cgaacaatgc gaagaaggac tcgtcgccct ggttcgcggc
    12241 gccacggacg agccgcgccg gctccgacgc gggcagccgc ggcaggcgca tcagctcgat
    12301 gatgaactgc gcgtaggtgg cgtagatgcg ccgcgccagg tgacgcacgc ggcggtcgct
    12361 cgccggcagc tgcaggacgt gcgacgcgtt ggccaggatg atgcgccgct tcgtcggcca
    12421 cgcgaaatac gagccgacga gcagggcctt gccgacgggc aggctgagcc gcgccgggat
    12481 gcgcccgatg gcggccgcgg cggcgcggta gccacgcacc gccgagcgct cacgcgcccg
    12541 gcggacggcc tggccaatgc ggcttgatct ggcctcgcgg cggtgcggcg cgtacgccac
    12601 accgcccttc ggctggttca cggcgatggt gttacctcgc cttcgcctca accttggctg
    12661 ccgcggcggt ggccgcagcc ttggcattgg ccgacgtctg cgacttggtc gcgacgcggt
    12721 cgtgcgcgcc cagccgacgc cgcttgggag cgggcgcctt ctcggtgcca acgagcgtct
    12781 cgttgtcctc gccgcggatg aacgcttcca cgcggttgaa cgcgatgtcg tcgtggatct
    12841 ggcgcggcgg gctcttcatg aagtagctcg acggtgccac gagcgtgccc ttcaggccgc
    12901 ggtcgaggcc cagcttgatg cagcggatgg cgtcggtgat gacgccggcg ctgttgggcg
    12961 agtcccagac ctcgagc
//
LOCUS       DATBRX010000198         5112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_77992, whole genome shotgun sequence.
ACCESSION   DATBRX010000198 DATBRX010000000
VERSION     DATBRX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77992"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VIF08_07720"
     CDS             complement(<1..1445)
                     /locus_tag="VIF08_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135916.1"
                     /translation="MVLTDKQAGRSTDTDVLFDAAQADFVRWEVVKDLIDSSIDLMLN
                     YRQSGHPGGSRSKVHQMVALLLSGAMRWDVRRPWLPFGDRFVLSAGHTVPLVYATLAV
                     MNEALRARHERDHDERFAFPDDGRWALTWEQLLKLRRRGGLPGHAEMEGKTLFLKFNT
                     GPSGHGMPPAAGEALALKLAGADEVKVFVMEGEGGLTPGAAHETRHTAWGLGLNNLVF
                     LVDWNDFGIDERPLSSVVYGTPEDWFVPYGWRVNGTEEGSEWGPVTQAVLEVARGTNP
                     GKRPSAAWFKTRKGRGYGKYDYKSHGTPWPMNAPEFWAVRKEFAGKYGITYEGVDEPA
                     PTDTDARNAQARANLEATFGVLRNDDALLTWLSDRLLEVAATVPEQVDGLNIGGKGTD
                     IFSDKKIFTAADYPAELWKQPGDKQPNRAALGNWGAWVNSYARAEYGRPLFIGASADL
                     AESTNLAGFGKDFGDLPGWGWYERDSNPRGTV"
     gene            complement(1587..2153)
                     /locus_tag="VIF08_07725"
     CDS             complement(1587..2153)
                     /locus_tag="VIF08_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEY8135917.1"
                     /translation="MAIAIEGAIVARPAPNTGAHTRRLFVLVMALGMALTVAFSQVTT
                     VAAGGGSDAEPARGSQAAAILRMVRDAVGHPFRIGTEGPKFFDCSGLVWSVYKRAGVP
                     NRIGGVRGTVTRYYKWAVAHGKISRRNPQVGDVVIWGSHGKLHHSGIYIGNGRAISAL
                     NPKLGVRNHPLGYVHMRVWGFIHTGISR"
     gene            complement(2472..2981)
                     /locus_tag="VIF08_07730"
     CDS             complement(2472..2981)
                     /locus_tag="VIF08_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135918.1"
                     /translation="MTDLHTWIVFIHVAGVIVFLVAHGVSAGVVLRLRTERDPAAVRT
                     LVELSRGSMMAMSVGALIWLVGGIVAGFTGKGGAGWWTTGSYWIWASLVLAIVIIGLM
                     TPLGRLYLNRVRAAVGVDVKTGAVDPGFQVDPAALEAAVKSGNPTLLAGIGVGGLLIL
                     LWLMIFKPF"
     gene            complement(3047..3475)
                     /locus_tag="VIF08_07735"
     CDS             complement(3047..3475)
                     /locus_tag="VIF08_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEY8135919.1"
                     /translation="MKLYFAAPLFSAAEFHWNAALAAALRAAGHEVYLPQENEPGKSA
                     PEIFAVDVAGVDWAEGIIAIMDGPDPDSGTAWEVGYAYGKGKPIVLIRTDIRSASSGG
                     AGYNAMLAEAATIRVDLPLPSLAEVAAAVIAALGRLETPR"
     gene            3521..3595
                     /locus_tag="VIF08_07740"
     tRNA            3521..3595
                     /locus_tag="VIF08_07740"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3553..3555,aa:Lys,seq:ttt)
     gene            complement(3880..4257)
                     /locus_tag="VIF08_07745"
     CDS             complement(3880..4257)
                     /locus_tag="VIF08_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135920.1"
                     /translation="MVATLVLVATGCAQRADYQLFVRNHSSEQWLIRTSVGDAGMQRV
                     VLVRPGSEGLALNWVGAREKEIELLMLDCTVVGTFEFTVDDQVDVPGVAGVDGSVQPF
                     GSNGDWNVSGIALTSACGGDLSM"
     gene            complement(4362..>5112)
                     /locus_tag="VIF08_07750"
     CDS             complement(4362..>5112)
                     /locus_tag="VIF08_07750"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEY8135921.1"
                     /translation="RELIAEGATADEARQAMAMFNSRGLVHTGRDDLADDQVPHAVDP
                     SPLLAAGLSPAQLDDPVAVAHAHKPTILIGTTGCRGAFSEPLIRELAANHEHPIILPL
                     SNPDVNAEARAEDVIEWTGGRAIVATGSPSRDVEFGGRRRTIGQANNVFIFPGMGLGA
                     VVAQARELTDEAFLVAARTLAGIVGDDALVNGMIYPPVSTLRPVARAIAEALVVHFRD
                     AGYGRQLRDEQVAEAVDKAMWRPEYLDYAAD"
BASE COUNT          822 a         1770 c         1671 g          849 t
ORIGIN      
        1 accgtgccgc gcgggttgga atcgcgttcg taccagcccc atccagggag gtcgccgaag
       61 tccttgccga atccggccag gttggtcgat tcggcgaggt cggctgaggc accgatgaac
      121 agcggtcggc cgtactccgc cctggcatac gagttcaccc acgcgcccca gttaccgagc
      181 gcggcgcgat tgggctgctt gtcaccgggc tgcttccaga gctcggccgg gtagtcggct
      241 gcagtgaaga tcttcttgtc ggagaagatg tccgtgcctt tgccaccgat attcaggccg
      301 tcgacctgct ccgggacggt ggccgccacc tcgagcagtc ggtcggagag ccaggtcaac
      361 agggcatcgt cgttgcggag gacgccaaag gtcgcctcga ggttggcgcg cgcctgggcg
      421 ttgcgcgcgt cggtatccgt gggcgcgggc tcgtccacgc cctcgtacgt gatgccgtac
      481 ttccccgcga actccttgcg cacggcccag aactcgggcg cgttcatggg ccacggagtg
      541 ccgtggctct tgtagtcgta cttgccgtag ccgcggccct tgcgcgtctt gaaccacgcg
      601 gcgctgggcc gtttgcccgg attggtgccg cgcgccacct ccaggaccgc ctgcgtgacc
      661 ggaccccact cggagccctc ttccgtgccg ttgacgcgcc agccgtaggg cacgaaccag
      721 tcctcgggcg tgccgtacac caccgacgac aggggccgct cgtcgatgcc gaagtcgttc
      781 cagtcgacga ggaacacgag gttgttgagc cccaggcccc acgccgtgtg tcgtgtctcg
      841 tgcgcggcgc ccggagtcag gccgccctcg ccctccatga cgaagacctt gacttcatcc
      901 gcgccggcaa gcttgagcgc gagcgcctcg cccgcggcgg gcggcatgcc gtggcccgac
      961 gggccggtgt tgaacttcag gaagagggtc ttgccttcca tctcggcgtg gcccggcagc
     1021 ccgccgcggc ggcgcaactt caggagctgc tcccaggtga gcgcccagcg cccgtcatcg
     1081 gggaacgcga accgctcgtc gtggtcgcgc tcatggcgcg cgcggagcgc ctcgttcatg
     1141 accgcgaggg tggcgtacac gagcggcacg gtgtgcccgg cactcagcac gaagcggtcg
     1201 ccgaacggca gccacggccg gcgcacatcc cagcgcatcg cgcctgacag caacagcgcg
     1261 accatctgat ggaccttcga gcgtgagcca ccggggtggc cgctctggcg gtagttgagc
     1321 atcaggtcga tgctcgaatc gatcaggtcc ttgaccactt cccagcgcac gaaatcggcc
     1381 tgcgcagcgt cgaaaagcac gtccgtgtcg gtcgacctgc cagcctgctt gtcggtcagc
     1441 accatgtcag caggatgcta catggccctg tcgggcgggc ccacgtgccg ttcggctcag
     1501 gggcggaggc tgagcggggc aaaaagaaac gcggccggct tacgccgacc gcgtctctct
     1561 ggacgggttc gtacggagcc cgctgctcag cggctgatgc cggtgtggat gaagccccag
     1621 acgcgcatgt gcacgtagcc cagggggtgg ttgcgcacgc ccagcttggg gttgagcgcg
     1681 ctgatcgcac ggccattgcc gatgtagatc cccgaatggt gaagcttgcc gtgcgagccc
     1741 cagatgacga catcgccgac ctgcgggttg cggcggctga tcttgccatg cgcgaccgcc
     1801 cacttgtagt agcgggtgac tgtgccccgg acgccaccga tgcgattcgg aacgccagcg
     1861 cgcttgtaga cgctccagac gaggcccgag cagtcgaaga acttgggacc ctccgtgccg
     1921 atgcggaacg ggtgaccgac cgcgtcacgg accatgcgca ggatggccgc ggcctggctg
     1981 cccctggcgg gttcggcgtc gctgccgccg ccggcggcaa cagtggtgac ctggctgaac
     2041 gctacggtca gggccatgcc gagggccatt accagaacga acaggcggcg ggtgtgggcg
     2101 cccgtgttcg gggcagggcg ggcgacgatc gcgccctcga tggcgattgc caattgcgtc
     2161 tccttggatc ggccccggct ccaagtccgg tttcgatcga tggcggtcgc ggttcagggc
     2221 gtggcagacg gggggtcaga cgtgaccgga atgggccgag cacgctgggg cgggctcgaa
     2281 accgtctgta gctattggcg cgaggacgtc tggggacgct ttcctcggcc gctgctggcc
     2341 ttcctgacgg cggcctgttg cttcgcccca ccagcctctc tcggtggagc cccgggagtt
     2401 ttacacagtt gagccggttt gccaaccctt agcggcgttt tccttgcgcc cgcataacgg
     2461 tctgtgcgtg atcagaacgg cttgaagatc atcagccaga gcaggatcag cagcccgccc
     2521 acgccgattc ccgccaggag cgtcggattg cccgacttga ctgcagcctc caacgctgcc
     2581 gggtcgacct ggaatcctgg gtcgaccgcc cccgtcttga cgtccacgcc gaccgcggcc
     2641 cggacccggt tcaggtacag ccggcccagc ggcgtcatca gcccgatgat cacgatcgcg
     2701 agcaccagcg acgcccagat ccagtagctg ccggtcgtcc accagcctgc gccgcccttg
     2761 cccgtgaagc cggccacgat gccgccgacg agccagatca gcgcgcccac gctcatcgcc
     2821 atcatgctcc cccgcgacag ctcgaccaac gtccgcacag cggccgggtc acgctccgtg
     2881 cgcagccgca gcacgacgcc ggctgatacg ccgtgggcga ccaggaagac gatcaccccc
     2941 gcgacgtgga tgaaaacgat ccaggtgtgc agatcagtca cgccctcacc tcacccgttg
     3001 tctggtcccc gccgatttcg gccgatgtta gtgcggccga cccctctcag cgcggcgttt
     3061 ccagacgccc aagcgcagcg atgaccgcgg ctgcgacctc ggccaaagaa ggtagcggta
     3121 ggtcgacgcg gatggtcgct gcctcggcaa gcatcgcgtt gtagccggcg ccgccgctcg
     3181 atgcgctccg gatgtcggtc cggatgagca cgatcggctt gccctttccg taggcgtagc
     3241 cgacttccca cgcggtgccc gaatcgggat ccggaccgtc catgatggcg atgatgccct
     3301 ctgcccagtc gacgccggcc acgtcgaccg cgaagatctc cggcgcggac ttgcccggct
     3361 cgttctcctg gggcaggtag acctcgtgac ctgccgccct caaggcagcc gcgagggcgg
     3421 cgttccagtg aaactcagcg gccgaaaaca gtggagcggc gaagtacaac ttcatgcgtc
     3481 cgcagtatcg ccctgggcag cgcggctaat ctgatcgctt gggcccatag ctcagcggca
     3541 gagcagggga cttttaatcc tcgtgtcctg ggttcgaatc ccagtgggcc ctccaactca
     3601 cgctcaccaa gacccgccaa gaccgcccgg tccaccccgt ccgcgactct ggctgtcaaa
     3661 ttaggttgtc gagcagacac ccccgttccg tccgctccac gacgatgccg gattaaaggg
     3721 cgagtctcct gactgccacc ctgatcggcg gcaacgaccg cctagtcgcg gcacgacgtc
     3781 tcggctggaa gcgccgtggc tcgatcttga cgcccaccag cgcatctcct ggacgcgtcc
     3841 tcaaccgcat tgcaggtgcg cggctgcccc acggccgtcc tacatagaca agtcgccacc
     3901 acatgctgag gtgagcgcga tcccacttac attccaatct ccattcgaac cgaaaggctg
     3961 taccgatccg tcaacgcccg ccacgccagg cacgtccact tgatcatcca cggtgaactc
     4021 gaaggttccg acaacggtgc aatccaacat aagaagttcg atctctttct cgcgcgctcc
     4081 cacccaattg agcgccagtc cctcgctccc tggccgcacg agcaccacgc gctgcatgcc
     4141 ggcatcgccc acactggttc ggatcagcca ctgttccgat gagtggttac gaacgaagag
     4201 ctgatagtcc gcgcgctggg cgcacccagt ggcgacaagg accagggttg cgaccaaagc
     4261 cagtagccag tgtcgtctcc tgctacaaaa gctcgcgtgg actgtcacag cggcgcccaa
     4321 attccccatg gttcctgctc ccagagttcg cagcgattcg gtcagtccgc ggcgtagtcc
     4381 aggtactcgg gccgccacat agccttatca acggcctcgg cgacctgctc atcgcgcagc
     4441 tgccggccgt agccggcgtc gcggaagtgg acgaccagcg cctcggcgat cgcccgcgcc
     4501 accggtcgca gcgtgctgac cggcgggtag atcatgccgt tcaccagggc atcgtcaccc
     4561 acgatcccgg ccaacgtccg cgcggcgacc aggaaggcct cgtcggtcag ctcgcgtgcc
     4621 tgggcgacca cggcgccgag gcccatgccg gggaagatga acacgttgtt ggcctggccg
     4681 atcgtccgtc gccgaccgcc gaactcgaca tcgcggctgg ggctgcccgt ggcgacgatg
     4741 gcgcggccgc cggtccactc gatcacgtct tccgcgcgag cctcagcgtt cacgtccggg
     4801 ttggacagcg gcaggatgat cgggtgctca tggttcgcgg cgagctcgcg gatgagcggc
     4861 tctgagaagg cgccgcggca gccggtcgtg ccgatgagga tcgttggctt gtgggcatgc
     4921 gccacggcca cgggatcgtc cagctgcgcg gggctcagcc cggccgccag cagggggctg
     4981 gggtcgacgg cgtgcgggac ctggtcatcg gccagatcgt cgcgaccggt gtggaccaga
     5041 ccgcggctgt tgaacatggc catggcctgg cgcgcttcgt cggccgtcgc gccctccgcg
     5101 atcagctcgc gg
//
LOCUS       DATBRX010000199         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_415257, whole genome shotgun sequence.
ACCESSION   DATBRX010000199 DATBRX010000000
VERSION     DATBRX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415257"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VIF08_07755"
     CDS             complement(<1..454)
                     /locus_tag="VIF08_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135922.1"
                     /translation="MTVAARPEVLAAIDIGSSSINVLVATSSLERIDQRSELVGLAEA
                     IERQGRIEPEDRGLILNALEDYIDLARAAGAARITLVGTEPLRRAANASEMVALVRKM
                     TGLPLRIVTVEQEAQLAFIGACAGRVPDRPTAVLDIGSSMTLISLHSAG"
     gene            complement(451..1404)
                     /locus_tag="VIF08_07760"
     CDS             complement(451..1404)
                     /locus_tag="VIF08_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135923.1"
                     /translation="MGKRRQQHIGAAIDVGSNSVHLLVAALAPPQTLVRRGLTTIDDR
                     SQLLGLGEVVDRLGHIPEEQRGQIMTGIAEYLLTAGTAGAEHVTLLGTEPFRRAADGH
                     EVAVEVGVVTGLPLHVINEQTEGLLTFIGVTGGVPPAEPLVVVDIGGGSTEMSIYSPG
                     KPLRLLPLAIGSARLTRAVVAHDPPTKSEFNKLERAARAAAAKLPQMRWRAKGRPRAI
                     FVGGTATNLARLGVLTRAGLAEDRKTLAKLTSAQVIEHFGVRPQRAVQLAAGAAIVDA
                     LLERAGLDEALVSDASLRDGAIIAAARFGDAWPDRLPEILG"
     gene            1452..>1682
                     /locus_tag="VIF08_07765"
     CDS             1452..>1682
                     /locus_tag="VIF08_07765"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HEY8135924.1"
                     /translation="MSSFITGREYMAPVRIHADRAAATLELEWPDGHVTRYDFETLRW
                     LCPCAYCRGEAGIPGWLDSNPTLTPIQTQLTDL"
BASE COUNT          241 a          598 c          564 g          279 t
ORIGIN      
        1 cgccggcgct gtggaggctg atcaaggtca tggagctgcc gatgtcaagc accgcggttg
       61 gtctgtcggg cacgcgtccg gcgcacgcac cgatgaacgc gagctgcgct tcctgctcca
      121 cggtcacgat gcgcagcggc agtccggtca tcttgcgtac cagcgccacc atctcgctcg
      181 cattggccgc gcggcgcaac ggctccgtgc cgacgagggt gatgcgcgcc gcgccggccg
      241 cccgggcgag atcgatgtag tcctcgagcg cgttgaggat gagaccgcga tcttcgggct
      301 cgatccggcc ttggcgttcg atcgcctcgg ccagtccgac cagctcgctg cgctggtcaa
      361 tgcgctccaa ggagctcgtt gccacgagga cgttaatgct gctgctgcct atgtcgatgg
      421 cagccagaac ttcgggccgc gccgccacgg tcaccccagg atctctggca ggcgatcggg
      481 ccacgcgtca ccgaagcggg cagcggcgat gatcgcgccg tcgcgcaggc tcgcgtcgga
      541 cacgagcgcc tcatcgagcc cagcgcgctc aagcagggcg tcaacgatcg ccgccccggc
      601 cgcgagctgc acggcgcgct gcgggcgaac gccaaagtgc tcgatcacct gcgcgctcgt
      661 cagcttggcc agagtcttcc gatcctccgc gagaccggca cgcgtcagca ctccgagccg
      721 cgccaggttg gtcgccgtgc cgccgacgaa gatcgcgcgc ggccgcccct tggcccgcca
      781 gcgcatctgg ggcagctttg ctgccgcggc gcgggccgcg cgttccagct tgttgaactc
      841 ggacttggtg ggtgggtcat gcgcgacgac ggcgcgcgtc aggcgggccg agccaatggc
      901 cagcggcagg agcctcagcg gcttgcctgg gctgtagatg ctcatctcag tcgagccgcc
      961 gccgatgtcg accacgacaa gcggctcggc cggcggcacg ccaccggtga cgccaatgaa
     1021 cgtcagcagg ccctccgtct gctcgttgat cacatgcagc ggcaggccgg tgaccacacc
     1081 cacttctacc gcgacttcgt ggccgtccgc cgcgcgtctg aacggctccg tgccgagcag
     1141 cgtcacgtgc tccgcgccag ctgtaccggc ggtcagcagg tactcggcga tgccggtcat
     1201 gatctggccg cgctgttcct cggggatgtg tcccagccgg tcgacgacct cgcccaggcc
     1261 gagcagctgg ctgcggtcgt cgatcgtggt cagcccacgc cggaccaggg tctggggtgg
     1321 cgcgagcgcc gccacgagca gatgaacgct gttgctgccg acgtcgatcg cggccccaat
     1381 gtgctgctga cgtcgtttgc ccaccgcgct cagtttcgcg ccttggcaat tccttggttt
     1441 accgttcctg cgtgagctcc ttcatcaccg ggcgcgagta catggcgcca gtccgcatcc
     1501 acgctgatcg tgccgcggcc accctcgagc tggagtggcc ggacggtcac gtgacgcgct
     1561 acgacttcga gacgctgcgc tggctgtgcc cgtgtgccta ctgccgcggc gaggcgggca
     1621 tcccgggctg gctcgattcg aacccgacac tgacacccat tcagacccag ctgaccgatc
     1681 tg
//
LOCUS       DATBRX010000200         7221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3654285, whole genome shotgun sequence.
ACCESSION   DATBRX010000200 DATBRX010000000
VERSION     DATBRX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3654285"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VIF08_07770"
     CDS             complement(<1..1827)
                     /locus_tag="VIF08_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8135925.1"
                     /translation="MAIDPNQEFLVQPVDASWLQCNIECQEACPVGTNCRGYLNLAAE
                     GRFEEGYILSREPNPVAAMCAYVCSAPCERACRRGDIDRPLAIRAMKRFLVEWHQASG
                     IPDDMPNITPRDTTVAVIGAGPAGIAVARELAVAGHQVTVFDELPYGGGTMLIGVPAF
                     RLPREAIEMDLKLVERLGVKFRYGVRVGRDITFEQLLQQHDAIAICAGAMNAVPLDVP
                     GGDLDGIQYGVDYMKKANLGEPLTVGQDVVIIGGGYTAMDCSRTSLRYGAKNVTIAYR
                     RTRSELVVDEEELGETEREGVRMEFLVSPVEVVGDENGKVRAVKFVRNRLGEPDATGR
                     RAPVPIEGSEFEIKADTVIPAVSQAADNTFLPVESSFEINRGRVRVDPGTYATNVRGV
                     FACGDFVTGPTTLIEAAGHGKKCAYAIDRYLSGRREVDVDANVRIVSSWRHEMPEFYD
                     VLPRVHVPMTAVAARMPSADPHVNFTTPVERGYTAPQAITEGTRCLMCNYNIWFDPFR
                     CVLCGACADVCPEGVIHMIDINQMKSEGALPELVDAYGWSEGAAMVLDEERCIRCALC
                     VKRCPYDAITMERFELQEISSDGRLYKESYRDTQLGLAGIGNG"
     gene            2014..3189
                     /locus_tag="VIF08_07775"
     CDS             2014..3189
                     /locus_tag="VIF08_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135926.1"
                     /translation="MVDVPARPPAAPAYPPLPGMGAGPCGPARAEVDYRCAEAERLAQ
                     AAQIQAQRLRDAKRMLARVEAQRDADARVRDRRLLADAKENARRAYRESYARSRNASP
                     SNDVQAAAGEWLREINRLNRQLELADRRAADVARQINELTAALPGIELAADAARIAAE
                     AAQVTCVDARGALAACEEDAGRHLRSARLAAAPATQATRTVVAPTGPAPISLLLGGDR
                     QALLGLTLRLAEETGVEAGRLQLLLLELRQAIAARALEDNALAFPAGHPFWSQFSAEG
                     SQQLALSLARLGYGFDGRDGWRDGRAPGVRELALALEYVGSDPRSLRRPAGQAAIDEL
                     WQGTRVLVEPWLAATVPDLALGRLIDVLGPRAARLGELWDMWGRLRPLLVAGVPTAG"
     gene            complement(3186..3938)
                     /locus_tag="VIF08_07780"
     CDS             complement(3186..3938)
                     /locus_tag="VIF08_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY8135927.1"
                     /translation="MTDPEPVAGAAPASAHTERPERALVIVAHPDDADFGVAGTMARW
                     IKAGTVARMVCCTSGDAGADDATIDPLALARTREAEQRAAAAVVGYEGVDFLHRPDGA
                     LENDLALREQLVRIIRQFKPDAVMAMDPTVIIHDDGFVQHVDHRNAGMAAIDAVYPAA
                     RNAMAFPHLVTAEGLAPWEVNQLYLFFTDNPNAWVDITDTVETKIAALREHTSQLRKP
                     EELEGMLRGWARENGERIGAAAAERFRWLKLG"
     gene            complement(4045..4560)
                     /locus_tag="VIF08_07785"
     CDS             complement(4045..4560)
                     /locus_tag="VIF08_07785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8135928.1"
                     /translation="MDPSTPSNMPGVGDTAPALSLPDDTGTLRNLAERRGHWTVLYFY
                     PKDDTPGCTTEACEFRDINSTITAHDAEVWGVSVLGSGSKAAFKRKFGLNFVLLADID
                     HAVAERYGTWVEKVNYGRKFMGIQRATFLIDPDGRIAQAWPKVKAQGHAADVLAALGD
                     ARAARAAAPVA"
     gene            complement(4578..5603)
                     /locus_tag="VIF08_07790"
     CDS             complement(4578..5603)
                     /locus_tag="VIF08_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8135929.1"
                     /translation="MLRVDDVRVVYGARAALDGVSLEVGAGERLAVLGPSGSGKSTLL
                     RAIAGLEPLTAGRIEWDGADMAGVPVHKRGFGLMFQDYALFPHRDVAGNVAFGLETRG
                     ATPTQRRERVDDVLTLVGLPGYGARRIDQLSGGEQQRVALARALAPTPRLLMLDEPLG
                     ALDRGLRTHLLDELVGLFGRLGLPLIYVTHDQEEALAVGDRVAVLNRGRLETVMTARD
                     LWLAPPNEFVARFLGLTNIVPIEPIDGRLTTPWGSLQAPDSPPGQPTRLLIRPEALTL
                     DPEGPLQGVVSAVAFRGDTTTVLIQPAGKAGATLEARLRSVAGALPKAGESVRLRVDP
                     AGVLFLP"
     gene            complement(5603..>7221)
                     /locus_tag="VIF08_07795"
     CDS             complement(5603..>7221)
                     /locus_tag="VIF08_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8135930.1"
                     /translation="ALTLALAALPLAFVGLFFVFPVASILVRGLAPNGVLDLAVPLQV
                     LTRPETRDIAWFTFWQAALSTLLTLAVGLPGAYVLARFNFPGRRLLNALVLVPFVLPT
                     VVVGSAFLALIGPSGALGVRLDHTIWAILAAHVFFNCAVVLRVVGGVWSQLDPRTEEA
                     ARVLGASRGQAFRQVTWPLLRPAVLSAASIVFLFCFTSFGAILILGGTDYATIEVAIY
                     RAAAVLLDLPAAATLALLQLVFLAVLLVVYSRLQARAAVGQRLAGARAGARPPRGRTE
                     WVLVAAAVSMLLAFLGLPIAALVERALAGSSGYGLDNFSALFGQSARGALFVPPIDAI
                     SNSLLFAAAATAICLPLGLLAAAVIAYRRGASATAFDALLMLPLGTSAVIVGFGFLVS
                     LGQLPIDLRTSVILIPIAHALVALPFVVRATVPLLRSIDRRLREAAAVLGASPARTWR
                     MVDLPMVGRALLVGGSFAFAVSLGEFGATLFIARPTAPTMPIAIFRLLSQPGALAFGQ
                     AMAMATVLMAVTAVAALAFDRLRAGSVSST"
BASE COUNT         1160 a         2479 c         2534 g         1048 t
ORIGIN      
        1 gccgttgccg atgcctgcca gaccaagctg agtgtcgcgg tagctctctt tgtagagccg
       61 accgtcggag ctgatctcct ggagctcgaa ccgctccatc gtaatagcgt catacggaca
      121 tcgcttgacg cacagtgcgc agcggatgca gcgctcctcg tcgagcacca tcgccgcgcc
      181 ctccgaccag ccgtacgcgt cgacaagctc gggcagcgcg ccctccgact tcatctggtt
      241 gatgtcgatc atgtggatca cgccctcggg gcagacgtcg gcgcacgcgc cgcacaacac
      301 gcaccggaac gggtcaaacc agatgttgta gttgcacatc aggcagcgcg tgccttcagt
      361 gatcgcctgc ggggcggtgt agccgcgctc gacgggcgtc gtgaagttga cgtgcggatc
      421 ggcgctgggc atgcgcgccg cgaccgcggt catgggcacg tggacgcgcg gcaggacgtc
      481 gtagaactcg ggcatctcgt ggcgccagct ggacacgatg cggacgttcg cgtcgacgtc
      541 gacctcgcgc cgcccggaca ggtagcgatc gatcgcgtag gcgcacttct tgccgtgacc
      601 ggcggcctcg atgagcgtcg tcggcccggt cacgaagtcg ccgcaggcaa agacgccgcg
      661 cacgttggtg gcgtacgtcc cgggatcgac gcggacgcgg ccgcggttga tctcgaagct
      721 cgactcgacc ggcaggaagg tgttatctgc ggcctggctg acggccggga tgacggtgtc
      781 ggccttgatc tcgaactcgc tgccctcgat gggcacgggc gcgcggcggc cggtcgcgtc
      841 gggctcgccc aggcggttgc ggacgaactt caccgcacgg accttgccgt tctcgtcgcc
      901 gaccacctcg accgggctga cgaggaactc catccgcacg ccttcgcgct ccgtctcgcc
      961 cagctcctct tcgtcgacga ccagctctga gcgcgtgcgt cggtaggcga tggtcacgtt
     1021 cttcgcgccg tagcgcaggc tcgtccgcga gcagtccatc gcggtgtagc caccgccgat
     1081 gatgaccacg tcctgaccca cggtcagtgg ctcgcccagg ttggccttct tcatgtaatc
     1141 gacgccgtac tggatgccgt ccagatcgcc accgggcacg tcgagtggca cggcgttcat
     1201 cgcaccggcg cagatggcga tggcgtcgtg ctgctgcagg agctgttcga aggtgatgtc
     1261 ccggccgact cggacgccat accggaactt gacgccgagg cgctccacca gcttgaggtc
     1321 catctcgatc gcctcgcgcg gcaggcggaa ggcgggaacg ccgatcagca tcgtgccgcc
     1381 gccgtacggc agctcatcga acaccgtcac ctggtggccg gcgacggcga gctcgcgggc
     1441 caccgcgatg ccagccggac ccgcgccgat gacggcgacc gtcgtgtcgc gcggcgtgat
     1501 gttgggcatg tcgtcgggga tgccgctggc ctggtgccat tcaacgagga agcgcttcat
     1561 ggcccggatc gcgagcggtc gatctatgtc gccgcggcgg caggcgcgtt cgcacggcgc
     1621 ggagcaaacg tacgcgcaca tcgccgcaac cgggtttggc tcgcgcgaga ggatgtagcc
     1681 ctcttcgaag cgcccctcag cggcgaggtt caggtagccg cggcagttcg tgccaacggg
     1741 gcacgcttcc tggcactcga tattgcactg gagccatgag gcatcgacgg gctgaaccag
     1801 gaactcctgg ttgggatcga tggccaagta gttcagacct cccgcggcag gctgggctcg
     1861 attgccaccg cgagtgatcg tagcagcggc cgcgcttggc ggcatgagca ggcccctgga
     1921 ctgcgctggc gatgtgcgga ccgaccacag gtacccacag actgctccac atgtcacatt
     1981 gtcgccctat ctggcgcgat ctaggctcga tggatggtcg acgtccctgc gcgcccgccg
     2041 gcagctcctg cctacccgcc tttgcccggc atgggcgccg gcccatgcgg gccggcgcgg
     2101 gcagaggttg actaccgctg cgcggaggcg gagcgcctcg cccaggccgc ccagatccag
     2161 gcgcagcggc tgcgcgacgc caagcgcatg ctcgcccgcg tcgaggcgca acgcgacgcc
     2221 gacgcgcgcg tgcgcgaccg ccgcctgttg gccgacgcca aggagaacgc gcggcgcgcg
     2281 taccgcgaga gctacgcgcg ctccaggaat gccagcccca gcaatgatgt ccaggccgcg
     2341 gccggcgagt ggctgcgcga gatcaatcgc ctcaaccgcc agctcgagct ggccgaccgg
     2401 cgcgcggccg acgtggcgcg ccagatcaat gagctcacgg cggccctgcc cgggatagag
     2461 ctcgcggcgg acgcggcgcg gatcgctgcc gaggccgccc aggtgacgtg cgtggacgcg
     2521 cgcggcgccc tggcggcctg cgaggaagac gcgggacgac acctccgttc tgcgcgcttg
     2581 gccgctgcac cagccacaca ggccaccaga accgtggtcg cgccaacagg accggcgcca
     2641 atctcgttgc tattgggcgg cgaccgccag gcattgctcg gcctgaccct gcgcctcgcc
     2701 gaggaaaccg gtgtcgaggc cggtcggctg cagcttcttc tgctcgagct acgccaagcg
     2761 atcgccgctc gcgctctcga ggacaacgcg ctcgcgttcc cggccggcca tccattctgg
     2821 agccagttct cagcggaggg cagccagcag ctggcgctca gcctggcccg gctgggctac
     2881 ggcttcgacg gtcgcgacgg ctggcgcgat ggccgcgcac cgggcgtgcg cgagctggcc
     2941 ctggctcttg aatacgtcgg cagcgatccc cgctcgctcc gccgacccgc cggccaagcg
     3001 gccatcgacg agctatggca gggcacgcgg gtgctggtcg agccgtggtt ggccgcgact
     3061 gtgcccgacc ttgcgctcgg tcggttgatc gatgtgctcg ggccgcgcgc cgcgcggctg
     3121 ggcgagctat gggacatgtg gggccgcctc cggccactcc tggtagccgg cgttccgacg
     3181 gccggctagc ccagcttcag ccaacggaag cgctcggccg cggcagcgcc gatacgctca
     3241 ccgttctcgc gggcccagcc gcgcagcatt ccctccagct cttcgggttt gcgcagctgg
     3301 ctcgtgtgct cgcgcagcgc ggcgatcttg gtctcgacgg tgtcggtgat gtccacccag
     3361 gcattcgggt tgtcggtgaa gaagaggtac agctggttga cttcccacgg cgcgaggccc
     3421 tccgccgtca ccaggtgcgg gaacgccatg gcattgcgcg ccgccggata cacggcatcg
     3481 atcgcggcca tgccggcgtt gcgatggtcg acgtgctgca cgaacccgtc gtcgtggatg
     3541 atcaccgtgg gatccatggc catgaccgcg tcgggcttga actggcggat gatgcgcacc
     3601 agctgctcgc gcagcgcgag gtcgttctcg agcgctccgt cgggccggtg caggaagtca
     3661 acaccctcgt agcccaccac ggcagcggca gcgcgctgct cagcctcacg cgtccgggcc
     3721 agggccagcg ggtcaatggt tgcgtcgtcc gcgccggcat cgccgctggt gcagcacacc
     3781 atgcgcgcca cggtgccagc cttgatccag cgcgccatcg tcccggcaac gccgaagtcg
     3841 gcgtcgtcgg ggtgggcgac gattacgagg gcgcgctcgg ggcgctccgt gtgggcagac
     3901 gccggcgcag cgccggcgac aggctccgga tcagtcactt tgaacctgta acttgtattt
     3961 cagtttcaac tttcggcgtg cttggcgccg gtataagatt tcgtccgagg catcgttcgt
     4021 ctccccccac gggcgatgcc tcatttacgc gaccggcgcc gcagcgcgcg ccgcccgggc
     4081 atccccgagc gcggcgagaa catcggccgc gtggccctgc gccttcacct tgggccaggc
     4141 ctgggcaatt cggccgtcgg gatcgatgag gaacgtggcc cgctgtatgc ccatgaactt
     4201 gcgcccgtag ttgacctttt cgacccacgt tccgtagcgc tcggcgacgg catggtcgat
     4261 atcggccagg agcacgaagt tgaggccgaa cttgcgcttg aacgcggcct tgctgccgct
     4321 gcccaggacg ctgacgcccc agacctccgc gtcatgcgcg gtgatcgtcg agttgatgtc
     4381 gcgaaactcg catgcctcgg tggtgcagcc gggcgtgtcg tccttgggat agaagtagag
     4441 caccgtccag tggccgcgtc gctccgccag gttgcgcagc gtgccggtgt catcgggcag
     4501 gctcaatgcg ggcgcggtgt cgcccacgcc gggcatgttc gagggcgttg aggggtccat
     4561 ggcggggatg ctagcgctca cggcaggaac agcacgcccg cgggatcgac gcgcagtcgc
     4621 accgattcac ccgcttttgg cagcgcgccg gccacgcttc gcaggcgggc ctccagcgtg
     4681 gcgcccgcct tgcctgcggg ctggatcaga acggtcgtgg tatcgccgcg gaaggcgacc
     4741 gcgctcacga cgccctgcaa cgggccttcg ggatcgagcg tgagggcttc gggccggatg
     4801 aggaggcggg ttggctgccc gggcggtgag tcgggcgcct gaaggctgcc ccagggggtg
     4861 gtgagtcggc cgtcaatcgg ctcgatcggg acgatgttcg tcaaccccag gaaccgcgcc
     4921 acgaactcgt tgggcggcgc gagccacagg tcgcgcgccg tcatgactgt ctcgaggcgg
     4981 ccgcggttga ggaccgccac ccggtcgccc acggcgagcg cttcctcctg gtcgtgcgtc
     5041 acgtagatga gcggcaggcc cagtcgcccg aacaggccga cgagctcgtc gagcaggtgg
     5101 gtgcgcagcc cgcgatccag ggcaccaagc ggttcgtcga gcatcaacag gcgcggcgtc
     5161 ggcgcgagtg cccgagccag cgcgacgcgc tgctgctcgc cgccggacag ctggtcgatc
     5221 cgccgcgcgc cgtagccggg cagcccgacc agcgtcagca cgtcatcgac gcgctctctg
     5281 cgctgcgttg gcgtagcgcc gcgcgtctcg agtccgaacg cgacgttgcc ggccacgtcg
     5341 cgatgcggaa agagcgcgta atcctggaac atcagcccga agccgcgctt gtggaccggc
     5401 acgccggcca tgtcggcgcc gtcccactcg atccgaccgg ccgtcagcgg ctccaggccg
     5461 gcgatcgccc gcagcaacgt tgatttgccc gatccacttg gacccagcac agccaggcgc
     5521 tccccggcgc caacctccag actgacgccg tcgagcgctg ccctggctcc gtaaacgacg
     5581 cggacgtcat caacgcgcag catcaggttg agctcacgct gccggcgcgc agccggtcaa
     5641 aggcaagcgc cgcgaccgcg gtcacggcca tcagcacagt tgccatggcc atcgcctggc
     5701 caaaggcgag ggcgcccggc tggctgagca ggcgaaagat cgcgatgggc atggtcggcg
     5761 cggttggtcg cgcgatgaag agcgtggccc cgaattcgcc cagcgaaacg gcgaacgcga
     5821 atgagccacc gaccagcagc gcccgaccga ccatgggcag gtcgaccatc cgccacgtcc
     5881 ttgccggcga cgcacccagg acggcggccg cttcacgcag gcggcgatcg atcgagcgca
     5941 gcagcgggac ggtggcgcgt acgacgaatg gcagtgcgac gagggcgtgg gcgatgggaa
     6001 tgaggatgac agaggtgcgc aggtcgatcg gcagctggcc cagcgaaacg aggaaaccga
     6061 aacccacgat caccgccgac gtgccgagcg ggagcatcag cagcgcgtcg aatgcggtcg
     6121 cggacgcgcc tctgcgatag gcgatgaccg ccgccgcgag caaaccaagc ggcaggcaga
     6181 tggccgtggc cgcggcggca aagaggagcg agttgctgat cgcgtcgatc ggcggcacga
     6241 acagtgcgcc gcgcgcgctt tggccgaaca gggcgctgaa gttgtcgagg ccgtagccgc
     6301 tcgatccggc gagggcgcgc tcgacgagtg cggcgatggg caggcccagg aaggcgagca
     6361 gcatgctcac ggctgccgcc acgagcaccc actctgtgcg gccgcgtggt ggtcgcgcgc
     6421 cagctcgtgc gcccgccagt cgctggccca ccgcggccct ggcctggagc cgcgagtaca
     6481 cgaccaacag gacagccagg aagaccagct gcaggagcgc cagggtggcc gcggccggca
     6541 ggtcgagcag cacggccgca gcgcgataga tcgccacttc gatcgtcgcg tagtccgtgc
     6601 cgcccaggat caggatcgcg ccaaacgacg tgaagcagaa caggaaaacg atggaagccg
     6661 cggacaggac ggcgggccgc agaagcggcc acgtcacctg gcggaacgcc tgcccgcggc
     6721 tggcacccag gacgcgcgcc gcttcctccg tgcgcggatc gagctgcgac cacacgccgc
     6781 caacgacacg cagcacgact gcgcagttga agaagacgtg cgccgcgagg atcgcccaga
     6841 tcgtgtggtc aagccgcacg ccgagcgcgc cgctcggtcc gatcagggcg aggaacgccg
     6901 agccgacgac gactgttggc aaaacgaacg gcacgagcac aagggcgttc agcagccggc
     6961 ggccgggaaa gttgaaccgt gccaggacgt atgcgccggg caggccaacg gcaagggtga
     7021 gcagtgtcga cagcgccgcc tgccagaacg tgaaccacgc gatgtcgcgc gtctccggcc
     7081 gcgtcagcac ctgcagcggc acagccaggt caagcacgcc gttcggggcg aggccgcgca
     7141 ccaggatcga cgcgaccgga aagacgaaga acagcccgac gaacgccagc ggcagcgccg
     7201 ccagcgcgag cgtcagtgcg t
//
LOCUS       DATBRX010000201         5886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3433024, whole genome shotgun sequence.
ACCESSION   DATBRX010000201 DATBRX010000000
VERSION     DATBRX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3433024"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..2264)
                     /locus_tag="VIF08_07800"
     CDS             complement(219..2264)
                     /locus_tag="VIF08_07800"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY8135931.1"
                     /translation="MTILANLSGPRDLRKMNEQQLEQLCGELRASIIQTVAKTGGHLG
                     SSLGVIELTVALHRNLESPVDRLVWDTGHQAYAHKLLTGRLEKFGTLRALGGVGGFPR
                     RTESEHDVMDGGHAGTGLSIAEGLALAREQMGTREKIVVVVGDAALISGMSLEALNDI
                     GHRRTKLLIVLNDNEMSISPTTGALSEYLSRIKLSRTYRGGKRLYQDVLGKLPVIGKP
                     ALELSKRIRSSVLSFAQPGQLFEDLGISYVGPIPGHNLRALNTVFRRALRDLDGPVIV
                     HVRTQKGRGYQPAIADQVSFHGAALPPMTVVPGGQMGAPSPAAVAGNGDGEATPSMAQ
                     AVAEKKKAPNYTAVASAELIALAKDDPRIVAITAGMPTGTGLAKFSDAYPGRMYDVGI
                     SEQHAMTMATGLALGGMRPYVAIYSTFLQRAFDQMVHDVCQNDAGVVIGVDRAGLVGE
                     DGTSHQGMFTVAAQRQLPNLIVASPRDEQELRRLMHTAFGQDHPFALHYPRDPGFDLP
                     EVAPTPIPVGQAEVMREGTDILICGFGPIVMRGAEVADLLTAEGWSVGLVNARFAKPL
                     DAATILTQARGKKLVVTLEEGVATGGFGAAVLEALADAALSDESLRSIPVKVIGLPRD
                     HFVDHGSVGDLRRYLRIDVPGIAEQVHEALAELGLANAEAKPAAATRRRSTPKAKTA"
     gene            complement(2408..2629)
                     /locus_tag="VIF08_07805"
     CDS             complement(2408..2629)
                     /locus_tag="VIF08_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135932.1"
                     /translation="MVPDQPPGKIAPDDCGVVAAVLGAPVRLLDPVGLLDPVGLLDPV
                     RPAEPPQATAEIQSSAATTDAGVRVMSGL"
     gene            2628..3338
                     /locus_tag="VIF08_07810"
     CDS             2628..3338
                     /locus_tag="VIF08_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135933.1"
                     /translation="MSFQASTSVDTTETSSSGEPGSIFYSTLTIHTVIQVSASDALTL
                     SGMDDEESALYGIGSVDLTGSGSNSGNALEQYIYTSDEHNALGCHYLDETGTEVNGSW
                     SQPGIAQGTIRFSEDGSYTIEVRASNTDVSTGEYTNPELPKTLWQTYTILEGAATDCP
                     APGRSEVEDTEGPIYDWASSVAYGTIGGQIDPANPPSVIDGSVTLVSESPEATVTVTW
                     HFVHDGPIVVTPTTFPEE"
     gene            complement(3339..3632)
                     /gene="xseB"
                     /locus_tag="VIF08_07815"
     CDS             complement(3339..3632)
                     /gene="xseB"
                     /locus_tag="VIF08_07815"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY8135934.1"
                     /translation="MSDPATNTGPNDAERQRIAALPFDKALTEFKSVVEKLEAGNLPL
                     EQSIALFEQGVLLQRRCEKMLSEAELRFQRLVEQAGGKVRALDLSLGESDEPG"
     gene            complement(3629..4975)
                     /gene="xseA"
                     /locus_tag="VIF08_07820"
     CDS             complement(3629..4975)
                     /gene="xseA"
                     /locus_tag="VIF08_07820"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY8135935.1"
                     /translation="MREPIQPMQPQQAESKAASLPILSVGDLTALVKNTLRASPKLRE
                     VWVEGEVGQVSISAAGHCYFTLKDERAQLKCVIFRDDRLMMPFEARTGLRLIAQGNID
                     VFDAQGAYQLYVRSLQPSGFGDLALQFEALKAKLAAEGLFEQARKRPLPPMPQNIGVV
                     TSLNGAVLHDIRRVIARRWPMARLVVSACQVQGQGAAATIVAALRRVSRWTDEATGRA
                     TDVVILARGGGSLEDLWPFNDEQVVRAVAAHPCPVVVGVGHETDVTLAEFAADVRAPT
                     PSVAAELVVPARVDELARVNQLGSRLNGALYQTLTGLRSQLTTEARQLEARHPQAQIT
                     ADRERIGNLLDRAARLVARRLEVERTTLGRASDDLPALAAGALAIARGELGRESAALN
                     ALSPYATLERGYSIVRGPDGTVLRDANNAKSGDQISVRLQRGEIGARVESVRDSTP"
     gene            complement(5374..>5886)
                     /gene="efp"
                     /locus_tag="VIF08_07825"
     CDS             complement(5374..>5886)
                     /gene="efp"
                     /locus_tag="VIF08_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY8135936.1"
                     /translation="DGEIWQILDYHHIKMGRGSAQVKIKLRNVKRGQTVERSFQAGDK
                     WPRAYLENRQVQFLYGDGNDFNFMDTSSYEQFSMTREQLGDAAQYLVEGMMVDRTSYE
                     GETIGVELPVTVDLKVVETEPGFAGDTASGARKPATTESGLVVQVPLFVQIGDTIRID
                     TRTGDYQTRV"
BASE COUNT          939 a         1990 c         1974 g          983 t
ORIGIN      
        1 ctcgccgccg cgcgacacaa agcgcggccg ctcgaccatc tcgatcggaa tatcggccgg
       61 caccgattca ccgggcttgc ggtccagtcg accgccatcg ccctcaccca cacgaacctg
      121 cgcggccatc agcagccgcg cagcctggtc gcggtcatcg gccaggccgc gctcgacaat
      181 caggtcgtcg agccggacgc gcttcaggcg gacgatcttc aggcggtctt ggccttcggt
      241 gtgctgcggc gccgtgtggc agccgcgggc ttggcctctg cattggccag cccaagctcc
      301 gcgagcgcct cgtgcacctg ctcggcgatg ccgggcacgt cgatgcgcag gtagcgccgc
      361 agatccccca cggagccgtg gtcgacgaag tgatcgcgcg gcaaaccgat gaccttcacg
      421 ggaatcgagc gaagcgactc gtctgacagg gcggcatcgg ccagcgcctc gagcacggcc
      481 gcgccaaagc caccggtcgc gacgccttct tccagcgtca ccacgagctt cttgccgcgt
      541 gcctgggtga ggatcgtcgc cgcatcgagc ggcttcgcaa accgcgcgtt gaccaacccg
      601 accgaccagc cttccgcagt cagcagatcg gcgacctcgg ccccgcgcat cacgatcggc
      661 ccaaagccac agatcaggat gtctgtgccc tcgcgcatga cctccgcctg gccgacaggg
      721 atgggcgtcg gcgcaacctc gggcagatcg aagcccgggt cgcgcgggta atgcagcgca
      781 aacgggtggt cctggccgaa tgccgtgtgc atcagccggc gcagctcctg ctcgtccctg
      841 ggtgaggcca cgatcaggtt cggcagctgg cgctgcgcgg ccacagtgaa catgccctgg
      901 tggctcgtgc cgtcctcacc cacgagcccc gcccgatcga caccgatgac caccccggcg
      961 tcgttctgac acacgtcgtg aaccatctgg tcgaaggcgc gctgcaggaa ggtcgaatag
     1021 atcgccacgt aggggcgcat cccgcccagg gcaagtcccg tggccatggt catcgcgtgc
     1081 tgctcggaga tgccgacgtc gtacatgcgc ccggggtatg cgtcgctgaa cttggcgagg
     1141 ccggtgccgg tcggcatgcc cgcggtgatc gcgacgatgc gcgggtcgtc cttggcgagc
     1201 gcgatgagct ccgcagacgc gaccgcggtg tagttgggcg ccttcttctt ctccgccacg
     1261 gcctgcgcca tggacggcgt tgcctcgcca tcgccattgc cggcgaccgc tgccggtgag
     1321 ggcgcgccca tctggccgcc cggcacgacg gtcatgggcg gcagcgcggc cccgtggaat
     1381 gacacctggt cggcgatggc cggctggtag ccgcggccct tctgtgttcg aacgtggacg
     1441 atcaccgggc cgtcgaggtc gcgcagcgcc cggcggaaga cagtgttcag cgcgcgcaga
     1501 ttgtgaccgg ggatgggtcc gacgtacgag atccccaggt cctcgaagag ctggccgggc
     1561 tgagcaaacg acaggacgga tgatcggatg cgcttggaaa gctcgagcgc cggcttgccg
     1621 atgacgggca gcttgccgag gacatcctgg tacaaccgct tgcccccacg gtacgtacgc
     1681 gacagcttga tccggctcag gtattcgctc agcgccccgg tcgtgggcga gatgctcatc
     1741 tcgttgtcgt tgagcacgat cagcagcttg gtccggcgat ggccgatgtc gttgagcgcc
     1801 tcaagcgaca tgccgctgat aagcgccgcg tcacccacga cgacgacgat cttctccctc
     1861 gtgcccatct gctcgcgcgc cagtgcgaga ccctccgcga tcgacagccc cgtgccggcg
     1921 tgaccgccgt ccatgacgtc gtgctcgctc tccgtgcggc gcgggaagcc gcccacgcca
     1981 ccgagcgccc gcagggtgcc gaacttctcg agcctgcccg tcagcagctt gtgggcgtac
     2041 gcctggtggc cggtatccca gaccaggcga tcgaccggtg attcgaggtt gcgatggagc
     2101 gcgaccgtga gctcgatcac gcccaggctg ctgcccaagt gcccacccgt cttggcgacg
     2161 gtctggatga tggacgcacg cagttccccg cacagctgtt cgagctgctg ctcgttcatc
     2221 ttgcgcaggt cgcgcggtcc ggacaggttg gccagaatgg tcaagagggt ctccgcagcg
     2281 ggttcgaaac agggacgagc gcgggcgcct gagtgttgtg cgcgattcta ccgtcgccgc
     2341 aggcgacaac tgaaaggcca gcgacgttgt aatagttcgc tgcccgtgcg agcatcgcga
     2401 gactcgttca gagaccgctc atcactcgaa caccggcgtc agtcgtagcc gcactgcttt
     2461 gtatctcagc ggtcgcctgc ggaggctcgg cgggtctcac cgggtcgagc agcccaacgg
     2521 gatcgagcag ccctactgga tcgagcagcc taaccggcgc gccgagcacc gcggcgacca
     2581 cgccacagtc gtcgggtgcg atcttgcccg gtggctggtc tggaaccatg tcgtttcagg
     2641 cttcgacgag cgttgacacg accgagacct cctcatcggg cgagcccggc agcatcttct
     2701 actcgaccct gaccatccac accgtgattc aggtgtcagc ttctgacgcg ttgacgctca
     2761 gcggcatgga cgacgaggag tccgccctgt acgggatagg gtccgtcgac ttgaccggct
     2821 cgggcagcaa cagcgggaac gcgctcgagc agtacatcta cacgtccgac gagcacaacg
     2881 cactcggctg ccactacctc gacgaaaccg gcacagaggt aaacggatcg tggtcacagc
     2941 cgggcatcgc ccagggaacc atcaggttca gtgaggatgg ctcgtacacg atcgaggtgc
     3001 gggcgtcgaa cacagatgtg tccactggcg agtacaccaa cccggagctg ccaaagacgc
     3061 tatggcagac ctacacgatc ctcgagggcg ccgccacaga ctgccccgcg ccgggaagat
     3121 cggaggtcga ggacactgag ggtccgatct acgactgggc cagctcagtc gcctacggga
     3181 caatcggcgg ccagatcgac cccgccaatc ccccgtctgt cattgacggc tcggtgacgt
     3241 tggtgagcga gtcgcctgag gcgacggtca cggttacgtg gcacttcgtt cacgacggtc
     3301 cgatcgtcgt cacccccacg acctttccgg aagagtagct agccgggctc gtcggactcg
     3361 cccaggctga ggtcaagggc gcgcaccttg ccgccggcct gctcgaccag gcgctggaac
     3421 ctgagctccg cctcggacag catcttctcg cagcgccgct ggagcagcac tccctgctcg
     3481 aacagcgcga tcgattgctc cagcggcagg ttgcctgcct cgagcttctc gaccacgctc
     3541 ttgaactcgg tcagcgcctt gtcgaagggc agcgccgcga tgcgctggcg ctcggcgtcg
     3601 ttggggccgg tgttcgtcgc tgggtcgctc acggcgtcga gtctcgcacc gattcgacgc
     3661 gggcgccgat ctcgccgcgc tggaggcgga cgctgatctg gtcgcccgat ttagcgttgt
     3721 tcgcatcgcg gaggacggtg ccgtctgggc cgcgaacgat ggaatagccg cgctccaagg
     3781 tggcgtacgg gctgagcgca ttgagcgcgg ccgattcacg gcccagctct cccctggcga
     3841 tggcaagcgc gcctgctgcg agggccggca ggtcgtccga cgcgcggccc agggtcgtgc
     3901 gctccacttc gaggcggcgc gcgacgaggc gggcagcgcg atccagcaaa ttgccgatgc
     3961 gttcgcggtc ggccgtgatc tgtgcctgcg ggtggcgcgc ctccagctgc cgggcctcgg
     4021 tcgtgagttg tgatcggagg ccggtgagcg tctgatagag cgcgccattg agccgcgacc
     4081 cgagctgatt gacgcgcgcc agttcgtcga cgcgcgccgg gacgacaagc tccgcggcga
     4141 cgctgggggt gggcgcgcga acgtccgcgg caaactcggc cagcgtgacg tcggtctcgt
     4201 gaccgacgcc caccacgacg gggcacggat gcgccgcgac ggcgcgcacg acctgttcat
     4261 cattgaaggg ccacaggtct tcgagcgacc cgccgcctcg cgcgaggatc accacgtccg
     4321 tcgcgcggcc ggttgcctcg tcggtccagc gcgagacccg gcgcagcgcg gcgacgatcg
     4381 tggccgcggc tccctggccc tgcacctggc aggccgacac gacgagccgc gccattggcc
     4441 agcggcgcgc tatcacgcgc ctgatgtcgt gtaggaccgc gccattgagc gaagtgacca
     4501 cgccgatgtt ctgcggcatc ggtggcagcg gccgtttgcg cgcctgctcg aagagcccct
     4561 ccgccgcgag cttcgccttg agcgcctcga actgaagcgc gagatcgcca aagccgctcg
     4621 gctggagcga tcggacgtag agctggtagg caccctgagc gtcgaagaca tcgatgttgc
     4681 cctgggcgat gaggcgcaac cccgttcgcg cctcgaacgg catcatcaga cggtcgtcgc
     4741 ggaagatgac gcacttcagt tgggcgcgct cgtctttgag cgtgaagtag caatggcccg
     4801 cggcgctgat gctgacctgg ccgacctccc cctcgaccca cacctcgcgc agcttgggcg
     4861 aggcgcgcag cgtgttcttg acgagcgcgg tcaggtcacc gaccgacagg atgggcaggc
     4921 tggccgcttt gctttctgcc tgctgcggct gcatcggctg gatcggctct ctcacggccc
     4981 taccctagcg cgccggatat cgcgctcact tggacgctgc gcgtgacaaa catctgtcgg
     5041 ggctggcccg acgggccggg actggccgtt ccggcgacga gtggaacgct gggcgtgtca
     5101 gatcgcggct gggggcccgt ttgaggccgg acatcggacg tttggaacgc tgagcgtgcc
     5161 acggttgggc cgcggccctg tgtttatgcg gctcgtgact caacggatga ccgagtcggc
     5221 atcgcgggcc gttccttcgc gatctgatgc gtcaggtgaa tagggcgaag actgaacgcc
     5281 cagattgcgc ccgctcgcca ctcgcaagcg cgccccgttt atccgttgag tcgcccggtg
     5341 cacaaggcgc caccggccat ggccccgggc cgatcagacg cgcgtctggt agtcgcccgt
     5401 gcgggtgtcg atacggatcg tgtcgccgat ctggacgaag agcggcactt ggacgacgag
     5461 accgctctca gtcgtcgcgg gcttgcgcgc gccagaggcg gtgtcgccgg cgaagcccgg
     5521 ctcggtttcc acgaccttca ggtcaacggt taccggcagc tcgacgccga tggtctcgcc
     5581 ctcgtacgag gtgcggtcga ccatcatgcc ctcgaccagg tactgcgccg cgtcgccgag
     5641 ctgctcgcgc gtcatcgaga actgctcgta cgacgaggtg tccatgaagt tgaagtcgtt
     5701 gccgtcgcca tacaggaact ggacctggcg attctcaagg tacgcgcgcg gccatttgtc
     5761 gccggcctgg aacgaacgct ccacggtctg gccgcgctta acgttgcgca gcttgatctt
     5821 cacctgtgcc gagccgcggc ccatcttgat gtggtggtag tcgaggatct gccagatctc
     5881 gccatc
//
LOCUS       DATBRX010000202         9321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3096938, whole genome shotgun sequence.
ACCESSION   DATBRX010000202 DATBRX010000000
VERSION     DATBRX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096938"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1631)
                     /locus_tag="VIF08_07830"
     CDS             complement(33..1631)
                     /locus_tag="VIF08_07830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8135937.1"
                     /translation="MELQFLGAATTVTGSQFLLTTGQAKVLVDCGMFQGSPNEAIRNR
                     IPLAYDPAELDAILLTHAHLDHCGLIPYVVARGFKGPIYATAGTVELARLVLLDSGKL
                     QQEFAKRQARFARRNPGKAAEEELEQQAAFEAAVELSGEDSTADRSKMVVDDERGESW
                     MAPNPEVALRYQPPQMEVELDEPLYDERDAEQAIEHFRAVRYDTGVDVAPGVRAVYLD
                     AGHILGSAIIRVTATDSPDGPERTLVFSGDLGRRDTPIIRDPTDVSDADYVLMESTYG
                     GREHEPQDEAVRILAETVRLVAEHDGVLLVPSFAIGRTQEVVWQLDRLIAEGKIPHLP
                     LYLDSPMASRASDVYRRHAEYFDEQTRRLLEQRETPLDYPGQIITNSAKQSQAISRAK
                     RPYMIVASNGMLTGGRVVGHLRQLIGDPSAVLLFVGYQGKGTLGAHLQEGATQVRIDG
                     AMHEVRCQVRSISGFSAHADESELLDWVANFARGKQPGDAGFPRRVFIIHGDPAAQAA
                     LAPKVRALGFEVDIPRWHDVVTLD"
     gene            complement(2022..2675)
                     /locus_tag="VIF08_07835"
     CDS             complement(2022..2675)
                     /locus_tag="VIF08_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135938.1"
                     /translation="MDERALAITIGDTQTVYATTDPQDQDQLSELFGNRPKIQLREAE
                     GDVEGHALSDEVLVDVEGHAIALRLPTPADAAALRRALAVTAVTATLVTAGAIASLQG
                     QAQPAANVIDAPRSATQGAPAQAIRADEAQALREQSYANAQAAAQAAAMAESRSEIPT
                     AQDRADRAEALRQQSYASSQASSQATTPDADVAIPGPAQHAEEAENLREQSYLNQDE"
     gene            2832..3305
                     /locus_tag="VIF08_07840"
     CDS             2832..3305
                     /locus_tag="VIF08_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY8135939.1"
                     /translation="MDRTEVPPQHPGDSIERALAGYAQIVELMSAMRTPEFPDSHVTM
                     AQMRVLMLLSAIGEARMSDLAHKLGIAPSTLSSLVDRLVEGGLAARRDDPRDRRSVLV
                     ALAPAGSKVLDHFNELGTDALRALLEQLDAKDIVTVNKAIELLVAAARRLSEDPR"
     gene            3302..6619
                     /locus_tag="VIF08_07845"
     CDS             3302..6619
                     /locus_tag="VIF08_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8135940.1"
                     /translation="MSFLSALAVRQRSVVLLLSGALFIAGISAWGSLKQELLPDVDFP
                     VITIIAPYPGAGASDVATQVAEPIERSIQGIARLAEVRSTSANSIALVVAQFEFGTNV
                     KETRATIEENIRGLGLPQGVEPTVSALNINASPVIIASVAATAESSLDRAADIATNEI
                     LPAILALDGVASADLTGTLEQQLIITLDPAKMAAAGITSQQITGILQANNLTFPSGQL
                     PEGGTRIPVSTVGELTSVAQIENLLVGVRQGQTLPGATPDPGNPPIPVFLKDIATVTV
                     DDVATTGFARTNGQPSLTLTVTKTSSGNTVQVARDVQQVLDDAGARHPTEISVVTVQD
                     LSGFIVESQDGLLREGGLGALFAVLTIFLFLFSLRSTLVAAISIPLSVLSALVLMQVA
                     DISLNVMTLGGLAVAVGRVVDDAIVVLENIYRHRALGDDKLTAVTKGPREVARAITAS
                     TLTTVMVFLPIGFVGGLVSQLFLPFALTVTFALLASLVCALTVVPVLAYLFIGRVKLN
                     VDEDGEPRNSFWIRLYTPLIRGALRNRWTRWGVLALSTALFVASLTLVAGLPTQFINT
                     GSEKILGVTVIPPAGATSQAVLERATQAEALVINLPDVEIVQTSVPGEGDTGFGTVFA
                     ALQGQPANSARLTIRFDPSVDLAEKTTALADALAPIRSDGYDISVAQTAGFTNNNLSI
                     VVSGPDLDTVHVATDAVMAAIAGRPDLANLTSDLVEASPQIEVRVDPNKALQVGSTAA
                     QIAAEVRALLTPTTVGNVTLDPEGGARLIVRTDPAVATSVEQLRNVLVGVAARVPLSQ
                     VATVEQVNVQGSITRIDGAPAAQITAEVTSEDTGTVSREVGQQVDDLVASGTLPAGVT
                     VTMAGVTAQMNEAFSGLYTSMAVAILLVYVMMVLAFNSLLTPFIIMFSLPLATIGAFP
                     ALALTGRPIGVSSLIGFLMLIGIVVTNAIVLLDLVERLKAEGHSTRDALIEGGRTRVR
                     PILMTAFATILALIPLAAGFNQGSIIAAELGTVVIGGLFSSTFLTLLVVPVVYSLVDG
                     GKQTINRWLRRSPDEEPVLALAPAVAMAVPAIPDIPVVVEPPAPSALSGGGTTALPFG
                     QRKPGLVGRFTRWLLRG"
     gene            6655..6885
                     /locus_tag="VIF08_07850"
     CDS             6655..6885
                     /locus_tag="VIF08_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135941.1"
                     /translation="MKQMYHPDDLAAMDPLVLMRNLDHVRMTSRRLSYILQQQVHLYT
                     PEANKVRTEIDQYVEAERQIEWEMARRGLRNE"
     gene            complement(7106..7402)
                     /locus_tag="VIF08_07855"
     CDS             complement(7106..7402)
                     /locus_tag="VIF08_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135942.1"
                     /translation="MAIEPAPEGVDLALEFVRFCYRRRPVGWPLMYDEMSAVASHGLF
                     RGLGYADLAERGISLCLTELPRLAELTRRVIAEEIPAVEPLPELTTLTLVPATS"
     gene            complement(7726..7802)
                     /locus_tag="VIF08_07860"
     tRNA            complement(7726..7802)
                     /locus_tag="VIF08_07860"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7766..7768),aa:Glu,seq:ctc)
     gene            complement(7822..8751)
                     /locus_tag="VIF08_07865"
     CDS             complement(7822..8751)
                     /locus_tag="VIF08_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8135943.1"
                     /translation="MSDFDDSRPIDVTAPGGPGTGNGHGSGNGHDGTPADPRANRPIR
                     KDERIVSERGLYIESWLPERRSRRKPLYLLHGELGGSWLWHRFLGYFAPRGWEGHALN
                     LRGHYWSETADFADIDMNTYIDDVLAGAELMPRPPIVIGHGMGGLLAMRLAQAKPISA
                     LVLISPALPAQLLEPPPAHMVRLLPQRYQRELLGWEGPIDALQRANPDLTSDDVMRIQ
                     HMLGAESGAARRQMLEGVEVVREELADVPRLVIGGGLDQSFSDADSHRLAEWLNADYI
                     SFSDMSHYGLVTGENTADRVTDALRSFIESHRL"
     gene            complement(8759..>9321)
                     /locus_tag="VIF08_07870"
     CDS             complement(8759..>9321)
                     /locus_tag="VIF08_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135944.1"
                     /translation="TALRYRATSGAPPDADHVLALLYDAHSGGLALRPWHRVVADSLA
                     ARSFAGRVVRDAQDLVDQMKLAESETTPGIVGVWTRDGGMLLKISPDRSQELDVDILS
                     STLPQMIGATTDELNANGRLTYTSDAWQAIAAVDSGAAEVCFLVRPTPVESVLQVASA
                     GGFMPPKSTFFHPKAATGLVFNPLWP"
BASE COUNT         1435 a         3206 c         3109 g         1571 t
ORIGIN      
        1 ggttggacgt cggcggatga tcactgcctt gatcagtcga gggtcaccac gtcgtgccag
       61 cgtgggatgt cgacctcgaa gcccagcgcc ctgactttgg gggcgagtgc ggcctgcgcc
      121 gccgggtcgc cgtggatgat gaagacgcgc cgcgggaacc cagcatcgcc gggctgcttg
      181 ccgcgcgcga agttggcgac ccagtcgagc agctccgatt cgtcggcgtg ggccgagaag
      241 ccgctgatcg agcgcacctg gcacctgacc tcgtgcatcg caccgtcgat gcgcacctgc
      301 gtggcgcctt cctggaggtg tgcgccgagg gtccccttgc cctggtagcc cacgaacagc
      361 agcacggcgc ttggatcacc gatcagttgg cgcaggtgcc cgaccacccg cccgcctgtg
      421 agcatgccgt tggaggccac gatcatgtac gggcgcttgg cccgactgat ggcctgtgac
      481 tgcttcgcgc tattggtgat gatctgaccg gggtagtcga gcggcgtctc gcgctgctcg
      541 agcaggcgcc gcgtctgctc gtcgaagtac tcggcgtggc ggcggtagac gtcgctcgcc
      601 cgcgacgcca tgggggagtc gagatacaac ggcaagtgcg ggatcttccc ctccgcgatc
      661 agccgatcga gctgccagac gacttcctgg gtacggccga ttgcgaacga cggcaccagc
      721 agaacaccgt cgtgctcggc gacgaggcgc accgtctcag ccagtatgcg cacggcctcg
      781 tcctgcggct cgtgctcgcg cccgccgtaa gtcgattcca tcagcacgta gtcggcgtca
      841 ctcacgtccg tggggtcgcg gatgatcggg gtgtcgcgcc gcccaaggtc gcctgagaag
      901 accagggttc gctcggggcc gtcgggagaa tcggtggccg tgacccggat gatggccgag
      961 ccgaggatgt ggccggcatc gagatagacc gccctgacac cgggggcgac gtcgacgccg
     1021 gtgtcgtagc ggacggcgcg gaagtgctcg atcgcctgct cggcgtcgcg ctcgtcgtag
     1081 agcggttcgt cgagctcgac ctccatctgt ggcggctggt agcgcagcgc gacctcgggg
     1141 ttgggtgcca tccacgattc gccgcgctcg tcgtcgacca ccatcttcga tcgatctgcc
     1201 gtggaatcct cgccgctcag ttcgaccgcg gcctcgaacg ccgcctgctg ctcgagctct
     1261 tcttccgcgg ccttcccggg attgcggcgc gcgaacctgg cctggcgttt ggcgaactcc
     1321 tgctgcagct tgcccgagtc gagcagcacg aggcgcgcca gctcgacggt gccggccgtg
     1381 gcgtagatgg gccctttgaa gccgcgcgcc acgacgtatg ggatcaggcc gcaatggtcg
     1441 aggtgggcgt gcgtcagcag gatcgcgtcc agctcggccg ggtcataggc gagcggtatg
     1501 cggttgcgta tcgcctcgtt ggggctgccc tggaacatgc cgcagtccac gaggaccttg
     1561 gcctggccgg tcgtcagcag gaactgcgag ccggtgaccg tggtggccgc gcccaggaat
     1621 tgaagctcca tcagcgcgga tggtcgcacc ggcggccgtg aggtgcgccg cgggagcgcg
     1681 aatccagcca cctagctggc cgggcgccga tgtgagctac gttcacggat tcggcgtccg
     1741 cgagccgcag tcggcggtga tcgccccctc gtggctcggt tccaaggcct tctggctcga
     1801 tctgacctca gattcgaccc attagacggg gcccgtgacc actggccggc cgaaatccag
     1861 gcggcagaca gcttccgcta cctaacgggc ccgatttcgt gaatgtggct cccaggctgc
     1921 cagcggcgga ggcggttgcg cgccggcaat gtgcgcgctc gcgcggttgg gtgccgggcg
     1981 ggcgcgcgac caagcggtca gccgcgcgcc cgcggcgtcg gtcactcgtc ctggttgagg
     2041 tagctctgct cgcgcaggtt ttcggcctct tccgcgtgtt gagccggccc gggtatcgca
     2101 acgtcggcat ccggcgtcgt ggcctggctg ctcgcctgcg agctggcgta gctctgctgg
     2161 cgcagggctt cggcccggtc ggcgcgatcc tgtgccgttg ggatctcact gcgcgattcg
     2221 gccatcgccg ctgcctgggc ggccgcctgg gcgttggcgt agctctgctc acgcagggcc
     2281 tgcgcctcat cggctcgaat ggcctgcgca ggcgcgccct gggtagccga acgcggcgcg
     2341 tcgatgacat tcgccgcggg ttgcgcctgc ccctgcagcg atgcgatcgc tcctgcggtg
     2401 accagagttg ccgtgaccgc tgtgacagcg agtgctcgcc gcagcgccgc cgcgtcggcc
     2461 ggcgtcggca ggcgcaacgc gatcgcatgg ccctcgacat ccacgaggac ttcgtcggac
     2521 agcgcatggc cctcgacgtc gccctcggct tcgcggagct ggatcttcgg gcgattgccg
     2581 aagagctcgg atagctggtc ctgatcctgg ggatcagttg ttgcgtacac ggtctgcgtg
     2641 tcgccgatgg ttatcgcaag tgctcgctcg tccatggtgg ctcctctccg acgttgcgtt
     2701 acgggcgatt gtgcgccgga gggccggctc tggccagcct cgaggcgaca gcggatgtgt
     2761 cggctgcccg acacaggccg cccatttagc ccgggtgtcg gggtcggata tacttcgata
     2821 tcatcaaacc attggatcgc accgaagtac ccccgcagca ccctggcgac tcgatcgagc
     2881 gcgccctcgc cggctacgcc cagatcgtcg agctcatgtc ggccatgcgt acgccggagt
     2941 tcccggactc gcacgtgacg atggcccaga tgcgggtcct gatgctgctc tccgctatcg
     3001 gcgaggcgcg catgtcggac ctcgcccata agctgggaat cgccccatca acgctgagct
     3061 cgctcgtcga ccgcctcgtc gagggcggcc tcgcagccag acgcgacgac ccgcgcgacc
     3121 ggcgcagcgt tctcgtcgcg ctcgcgccgg cggggagcaa ggtgctcgac cacttcaatg
     3181 agctgggtac cgacgcgctg cgtgcgctgc tcgagcagct cgacgccaag gacatcgtca
     3241 cagtcaacaa agccattgag ctgctcgtcg ccgccgcgcg acgactttcg gaggatcccc
     3301 ggtgagcttc ctgtccgccc tggccgtgag gcagcgcagc gtcgtcctgc tgctgtccgg
     3361 cgcgctcttc attgccggca tctcagcgtg ggggtcgctc aagcaggagc tgctgcccga
     3421 cgtcgacttt cccgtcatca ccatcatcgc gccatacccc ggcgccggtg cgagcgacgt
     3481 cgcgacccag gtcgcggagc ccatcgagcg ctcgatccag ggcatcgcgc ggctggccga
     3541 ggttcgttcg acgagcgcca actcgattgc cctcgtcgtc gcccagttcg agttcggcac
     3601 gaacgtgaag gaaacgcggg cgacgatcga ggagaacatc cgcggtctgg gcctgccgca
     3661 gggcgtcgaa ccgacggtca gcgcgctcaa catcaacgcg tcgccggtca tcatcgcgtc
     3721 cgtcgccgcg accgctgaga gcagcctcga ccgcgcggcg gacatcgcca ccaacgagat
     3781 cctgcccgcg atcctggcgc tcgacggtgt ggccagcgcc gacctgaccg gcacgctcga
     3841 gcagcaactg atcatcacgc tcgacccggc aaagatggcg gcggccggca tcacgtccca
     3901 acagatcacc ggcatcctcc aggccaacaa cctgacattc ccgtcgggcc agctgcctga
     3961 gggcggcacg cgcatcccgg tctcgaccgt gggcgagctg acgtcggtcg cccagatcga
     4021 gaacctgttg gtcggggttc gtcaagggca gaccctgccc ggcgccacgc ctgatccggg
     4081 taatcccccg attcctgtct tcctcaagga catcgcgacg gtcaccgtcg atgacgttgc
     4141 gaccacgggg tttgcgcgga caaatggcca gcccagcctg acgctcaccg tcaccaagac
     4201 ctcatcgggc aacaccgtcc aagtcgcgcg cgacgtgcag caggtcctcg atgacgccgg
     4261 cgcgcgccac ccgactgaga tcagcgtcgt caccgtccag gacctgtccg gcttcatcgt
     4321 ggagtcgcag gacggcctgc tgcgcgaggg cggcctgggc gcgctcttcg cggtgctgac
     4381 gatcttcctg ttcctgttca gcctgcgctc gaccctggtc gccgcgatca gcatcccgct
     4441 cagcgtcctg tcggcgctcg tgctgatgca ggtcgccgac atctcgctca acgtcatgac
     4501 gctcggcggg ctcgcggtcg cagtgggccg cgtggtggac gacgcgatcg tggtgctgga
     4561 aaacatctat cgccacaggg cgctcggcga cgacaagctg accgccgtca ccaagggccc
     4621 gcgcgaagtc gcgcgcgcca tcaccgccag cacgctgacg accgtcatgg tcttcttgcc
     4681 catcgggttt gtcggtggcc tcgtcagcca gctcttcctg ccgttcgcgc tgaccgtcac
     4741 cttcgccctg ctcgcctcat tggtgtgcgc gctgaccgtc gtgccagtgc tggcctacct
     4801 gttcatcggc cgggtcaagc tcaacgtcga cgaggacggc gagcccagga actcgttctg
     4861 gatccgcctc tacacgccgc tcatccgcgg cgcgctgcgc aatcgctgga cgcgctgggg
     4921 cgtgctggcg ctctcgaccg cgctcttcgt ggcgtcgctc accctggtcg ccgggctgcc
     4981 cacccagttc atcaacaccg gctcggaaaa gatcctgggg gtgacggtca tcccgcccgc
     5041 cggcgcgacc tcgcaagcag tgctcgaacg agcaacccag gccgaggcac tggtgatcaa
     5101 cctgcccgac gtcgagatcg tccagacgag cgtcccgggc gagggcgaca ccggcttcgg
     5161 cacggtcttc gccgcgctcc agggccagcc cgccaactcg gcgcgcttga cgatccgctt
     5221 cgatccgagt gtcgatctgg cagaaaagac gacggcgctg gccgatgcac tcgcgcccat
     5281 ccgatccgac ggctacgaca tcagcgtcgc ccagaccgcc ggcttcacca acaacaacct
     5341 gagcatcgtc gtgtccgggc ctgacctgga cacggtgcac gtcgcgaccg atgcggtcat
     5401 ggccgcgatc gccgggcgac ccgacctggc caacctgacg tcggacctgg tcgaggcgag
     5461 cccccagatc gaggtgcgcg tcgatcccaa caaggcactg caggtcggct cgaccgccgc
     5521 ccagatcgcg gccgaggtgc gcgccctgct gacgccgacg accgtgggaa acgtgacgct
     5581 cgacccggag ggcggcgcga ggctcattgt ccgcaccgac cctgctgtcg cgacatcggt
     5641 tgagcagctg cgcaacgtgc tcgtgggcgt ggccgcgcgg gtgccgctca gccaggtggc
     5701 gaccgtcgag caggtcaacg tccagggctc gatcacgcgc atcgacggcg cgcccgcggc
     5761 gcagatcacg gccgaggtca ccagcgagga cacgggcacc gtgtcgcgcg aggttggcca
     5821 gcaggtcgac gacctcgtgg cgagcggcac attgccggcg ggcgtcacgg tcaccatggc
     5881 cggcgtcacc gcccagatga acgaggcctt cagcggcctc tacacgtcga tggccgtggc
     5941 catcctgctc gtgtacgtga tgatggtcct ggcgttcaac tcgctgctga ccccgttcat
     6001 catcatgttc agcctgcccc tcgcgacgat tggcgccttc ccggcgctgg ccctcaccgg
     6061 ccggccgatc ggcgtctcat cgctcatcgg cttcctgatg ctgatcggca tcgtcgtcac
     6121 caacgccatc gtgctgctcg accttgttga gcggctgaag gcggagggcc actcgacgcg
     6181 cgatgccctg atcgagggcg gacggacaag ggtccggcca atcctcatga cagcgttcgc
     6241 cacgatcctg gcgctcatcc cgctggcagc cggcttcaat cagggctcga tcatcgccgc
     6301 cgagctgggc accgtcgtca tcggtggcct tttcagctcg acgttcctga cgctgctcgt
     6361 cgtgcccgtc gtctattcac tggtcgacgg cggcaagcag acgatcaacc gctggttgcg
     6421 tcgatcaccc gacgaggaac ccgtcctggc cctggcccct gccgtcgcca tggcagtgcc
     6481 tgccatcccg gacatacccg tcgttgtcga gccacccgcc ccatcggcgc tatcgggtgg
     6541 cggcaccacg gcactgcctt ttggtcagcg caagccgggc ctggtcggcc gcttcacgcg
     6601 ctggttgttg cgcggctgac gccgcgcacg gccgacttat actcgggccg gctcatgaag
     6661 cagatgtacc accccgacga tctggcggcg atggatccgc tggtgctgat gcgcaatctc
     6721 gaccacgtgc gcatgaccag ccggcggctc tcgtacatcc tccagcagca ggttcacctc
     6781 tacacgccgg aggcgaacaa ggtccgcacc gagatcgatc aatacgtcga ggccgagcgc
     6841 cagatcgagt gggagatggc ccgccgcggc ctgcgcaacg agtaggcctt cgcacgcgaa
     6901 caccgtttgt ttccccgggc gaaagtaatc gccaagatcg aacgggtttg aggcccaact
     6961 tggccgcatc ttccacggcc gggaccaacc agccacgatc agcccgattt gagggcgcgc
     7021 ccagcggcta gttggctttt acatctaccg ggtgaaacaa aacgcggccg cttggcgggc
     7081 acggaggtgc gcgccccggc gcgagctagc tggtcgcggg aaccagggtc agggtcgtca
     7141 gctcggggag cggctcgacc gctggtattt cctccgcgat gacgcgccgc gtgagttccg
     7201 cgagccgcgg cagctcggtc aggcagagtg agatcccgcg ctcggcgagg tcggcgtagc
     7261 ccagcccgcg gaacagaccg tggctggcaa cggcgctcat ctcgtcgtac atgagcggcc
     7321 agccaaccgg ccggcggcgg tagcagaagc gtacgaattc gagcgccagg tccacgccct
     7381 cgggcgcagg ctcaatagcc acggtcaatc ctctcccctt gcatctcgcg cgggtgccgt
     7441 aagcgcgaga cgcttagcca acaaagccct gggatcgagc acggccgccg gctcgtccgc
     7501 ggcgctcgag tccctcctcc accacgtcgg ccggatctcg gtcgggggcg cgacgtcgcc
     7561 ggattgtagg cgcgattcgg acatttcgag aaggaggtat gtccaccttc ttgtccacca
     7621 tgttctccac agcgtggacg aaacggccgc gctcgcgact caacaggtgg catgaaattg
     7681 agacgccccc ggggcgcggt ccgggggcgt cgggtcgttc gtgtgtggta ccgcatatcg
     7741 gattcgaacc gatgatctcc gccttgagag ggcggtgtcc tgggcctcta gacgaatgcg
     7801 gcgaggcggc cgtagtttac ctcagagtcg atgcgactca atgaaagaac gcagtgcgtc
     7861 agtgaccctg tcggcggtgt tctcgcccgt caccaggccg tagtgcgaca tgtcgctgaa
     7921 cgagatgtag tcggcgttca accactccgc gagacggtgg ctgtcggcat ccgagaacga
     7981 ctggtcgaga ccaccaccga tgaccaggcg cggcacgtcc gcgagctcct cgcgcacgac
     8041 ctccacgccc tcgagcatct ggcgccgcgc ggcgcctgac tcggcgccca gcatatgctg
     8101 gatgcgcatc acatcgtccg atgtcaggtc ggggttggcg cgttggagcg catcgatcgg
     8161 cccttcccag cccagtagct cgcgctggta gcgctgcggc aacaggcgga ccatgtgcgc
     8221 cggcggcggc tcgagcagct gcgctggcag cgccgggctg atgagcacga gcgcgctgat
     8281 cggcttggcc tgcgcgagcc gcatagccag cagtccgccc atgccatgac cgatgacgat
     8341 gggcggccgc ggcatcagct ccgcgccggc aaggacatcg tcgatatagg tgttcatgtc
     8401 gatgtcggcg aagtcggcgg tctcgctcca gtagtgcccg cgcaggttca acgcgtggcc
     8461 ttcccagccg cgcggcgcaa agtagcccag gaagcggtgc cacagccatg agccgcccag
     8521 ctcgccgtgg agcaggtaga gcggcttgcg acgcgacctg cgttcgggca gccacgactc
     8581 gatgtacagg ccgcgctcgc tgacgatccg ctcgtccttg cggatgggcc gattggcgcg
     8641 cggatcagcc ggtgtaccgt cgtgaccatt gcccgagcca tggccgttgc ccgtccccgg
     8701 gccgcctggt gccgtgacgt cgatcggccg gctgtcgtcg aagtcgctca tcgcacgcct
     8761 aaggccagag cgggttgaag acgaggcccg tcgcggcctt gggatggaag aaggtcgact
     8821 tgggcggcat gaagccaccg gcgctcgcga cttgcagcac cgactcgacg ggcgtcggcc
     8881 gaaccagaaa gcacacctcc gcggcgccgg aatcgacagc ggcaatcgcc tgccaggcgt
     8941 cactcgtgta agtcaggcgg ccgttcgcgt tcagctcgtc cgtcgtggct ccgatcatct
     9001 gcggcagggt actcgacagg atgtcgacgt ccagctcctg cgatcggtcc ggggagatct
     9061 tcaggagcat cccgccatcg cgcgtccaca cgccgacgat tcctggcgtc gtttctgatt
     9121 cggccaactt catctggtcg accaggtcct gcgcgtcgcg gactacgcgg ccagcgaacg
     9181 atcgtgctgc aagcgagtcc gcgacgaccc gatgccacgg tcgcagagcg aggccgccgg
     9241 aatgagcgtc gtacagcagg gccagcacgt ggtccgcgtc cggcggagcg ccggaggtag
     9301 ctcgatagcg caacgcggtt t
//
LOCUS       DATBRX010000203         6711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1420703, whole genome shotgun sequence.
ACCESSION   DATBRX010000203 DATBRX010000000
VERSION     DATBRX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420703"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..329)
                     /gene="rpsR"
                     /locus_tag="VIF08_07875"
     CDS             complement(57..329)
                     /gene="rpsR"
                     /locus_tag="VIF08_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY8135945.1"
                     /translation="MPPAPSRSKKRSDEYRDRRRRKVCAFCADKTVLIDYKEINRLRR
                     YLSERAKIEPRRKTGTCAEHQRQLAVALKRARHVALLPYAPEHLRA"
     gene            complement(344..796)
                     /locus_tag="VIF08_07880"
     CDS             complement(344..796)
                     /locus_tag="VIF08_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8135946.1"
                     /translation="MSLNKCMIIGNLGRDPEMRYTPSGQAVTQFTVAVNRNYKDAQGE
                     RQEETEWFRVVAWGQQAEFSAEYLRKGAKVFVEGRIQTRQWEGQDGQKRYTTELVANT
                     IQNLERRPRDDAGDGGSQLPPEPRGQRPPAAKPAGEQAPEPDYDDLPF"
     gene            complement(816..1250)
                     /gene="rpsF"
                     /locus_tag="VIF08_07885"
     CDS             complement(816..1250)
                     /gene="rpsF"
                     /locus_tag="VIF08_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY8135947.1"
                     /translation="MHRYELMLVLRPDAPDDQVQAVIDRVTRSIAAAEGQIVKVSPWG
                     RRRLAYAIGQQREGSYFIIQFDAPGAAVIELERALNITEEVMRHLITRVEKPQARRAS
                     ADAGDGLADEEFVPPVDDEDEIPDTEFIDESESEAAPAATDR"
     gene            1391..2605
                     /locus_tag="VIF08_07890"
     CDS             1391..2605
                     /locus_tag="VIF08_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8135948.1"
                     /translation="MSSHVVLVSLGGNAQDLASALSRLDLSVSVAHDVNGVLLELADT
                     QLIVVEAPDTASLAMLCRRINDEAGSSHPPILAVVAASDVETRVKVLQAGADDVLAQP
                     IDERELAAMVDALLLRPAPAPPGRAPAASSSPARPQATPGRVIVFASAKGGSGTTTLA
                     VNTALVLAEMAPGNVAIADLDMFHGQVSTHLDIYGRASTATLAREDLATQTPEMITEA
                     GRLHPSGLMVYGGPYRPDDADDLTAGQLTSLVATLRGMYATVVVDGGSTLESRQMAVL
                     HAADRLALVVTPDIPALRLLHAALEVLSEMGNAADRTTFVVNDIYPKSSISADQIEEH
                     LSIRVGLTVPYDNENFLRAINEGQPIVTLSRRTHAAAAIRKLSEVLADNDTEEGAPRP
                     PKKGRLGGLLGR"
     gene            complement(2626..2712)
                     /locus_tag="VIF08_07895"
     tRNA            complement(2626..2712)
                     /locus_tag="VIF08_07895"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2676..2678),aa:Leu,seq:taa)
     gene            complement(3465..3540)
                     /locus_tag="VIF08_07900"
     tRNA            complement(3465..3540)
                     /locus_tag="VIF08_07900"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3505..3507),aa:Cys,seq:gca)
     gene            complement(3549..3622)
                     /locus_tag="VIF08_07905"
     tRNA            complement(3549..3622)
                     /locus_tag="VIF08_07905"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3588..3590),aa:Gln,seq:ttg)
     gene            complement(3655..3730)
                     /locus_tag="VIF08_07910"
     tRNA            complement(3655..3730)
                     /locus_tag="VIF08_07910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3695..3697),aa:Gly,seq:gcc)
     gene            complement(3762..3837)
                     /locus_tag="VIF08_07915"
     tRNA            complement(3762..3837)
                     /locus_tag="VIF08_07915"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3802..3804),aa:Phe,seq:gaa)
     gene            complement(3914..4906)
                     /locus_tag="VIF08_07920"
     CDS             complement(3914..4906)
                     /locus_tag="VIF08_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8135949.1"
                     /translation="MTAPVQSFYSQISANRRRSLLLVFLLTAFLGFFGFVIGFAMSGG
                     YWQGGVVATGIAVVIALLMTSVSYFAGDGIVLAASGAREVNDQVAPQLMNVVREMSIA
                     ANVPVPKVYAINDTAPNAFATGRDPKHASLAVTTGLLEKLDREELQGVIGHELAHVRN
                     LDIRFALLVATLVGSIALLADFFLRFTFWGGLGGRGSRDRGSGGGGLVIIAIVVGLVL
                     SIIAPFIARLVQLAVSRQREYLADSSSVELTRNPYGLERALVKISSDREVLEVANRAT
                     QHLYFVNPIKKFEARAAGLTATHPPIIDRVNRLRSLTGQAPVDERGLQALAGLD"
     gene            complement(4959..5537)
                     /locus_tag="VIF08_07925"
     CDS             complement(4959..5537)
                     /locus_tag="VIF08_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8135950.1"
                     /translation="MIIILILIVVVIVGGILIYNGLVQRRLRIDEAFAQIEVQLKRRW
                     DLIPNLINAVKGYMGFEQSVLTQVTEARANAVTAGAQGPAAQAAAENALTSTLRSLFA
                     TVENYPELKANQNVLELQEQLTTTENQIGFARQHYNATVLDYNTSIATFPNVLIAGPF
                     GFSKREFFDAEPEASQVPQVDLGTYNTPPKVG"
     gene            5670..>6711
                     /locus_tag="VIF08_07930"
     CDS             5670..>6711
                     /locus_tag="VIF08_07930"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8135951.1"
                     /translation="MSRKRISRRSLLRLGAAASVAFALSGRRALDVAASTLAGMPGSG
                     WLAGTTASAVPRTLYRAAAIADGRSPALARQMSVLVSGGRVAWIRPADAEEDPGPADG
                     LEIVDARGLTILPGLVDGHCHLTSPGGPDYIAHFEDAPSQLLRTAERNGQLSHAAGVA
                     WLREAGSPTVTDPTDGRRRALALGIRDRWAGRTDRPRVRAAGTWIQVKGGLARNLGIV
                     VSSGDELVAAVERQLDQGADLVKLYVAADNSRQSPWTPAQINRAVQAAHARGARVAAH
                     AMWLGQARAAVAGGVDSIEHGFHLDADLCAEMVRRGTYLVTTLSVPRSWLRIGSTTSG
                     TWWSTRAGRRFAR"
BASE COUNT         1054 a         2204 c         2308 g         1145 t
ORIGIN      
        1 aggagcgcga cgatattcac ggggccgtcc tcggctgtgg cgccgtcagg aagtggctag
       61 gcgcgcaggt gctccggggc gtacggcagc agcgcgacgt gacgggcgcg cttgagcgcg
      121 accgcgagct ggcgctggtg ctccgcgcag gtgccggtct tgcggcgcgg ttcgatcttg
      181 gcgcgctcgg acaggtagcg ccgcaaccgg ttgatctcct tgtagtcgat cagcacggtc
      241 ttgtcggcgc agaaggcgca aaccttgcgc cggcgccggt cgcggtactc gtcgctgcgc
      301 ttcttgctgc ggctgggggc ggggggcatt gctcaaagtc tccttagaag ggcaggtcgt
      361 cgtagtcggg ctcgggcgcc tgctcgccgg cgggcttggc ggccggcggg cgctggccgc
      421 gcggctccgg gggcagctgg ctgccgccat caccggcgtc gtcgcgcggg cgacgctcga
      481 ggttctggat cgtgttggcg acgagttcag tcgtgtagcg cttctggcca tcctggcctt
      541 cccactggcg cgtctggatg cggccctcga cgaacacctt ggcacccttg cgcaggtatt
      601 cggcgctgaa ctccgcctgc tggccccagg cgaccacgcg gaaccactcg gtctcttcct
      661 ggcgctcgcc ctgggcgtcc ttgtagttgc ggttgacggc gacggtgaac tgggtcaccg
      721 cctggccgct tggcgtgtag cgcatttccg gatcacgccc cagattgccg atgatcatgc
      781 acttgttgag cgacatagcg agttcctcct ggcgctcagc ggtccgtggc ggccggagca
      841 gcttcgctct cggactcgtc gatgaattcg gtgtcgggga tctcgtcctc gtcgtccacg
      901 ggtggcacga actcctcgtc ggccagcccg tcaccggcgt ccgctgacgc gcgtcgcgcc
      961 tgcggcttct cgacgcgggt gatcaggtgg cgcatcacct cctcggtgat gttgagggcg
     1021 cgctcgagct cgatcacggc ggcgccgggg gcgtcgaact ggatgatgaa atacgagccc
     1081 tcgcgctgct ggccgatcgc ataggccagc cgacggcggc cccacggact gaccttcacg
     1141 atctggccct cggcggcggc aatcgaacgc gtgacgcggt cgatcacggc ctggacctga
     1201 tcgtcgggcg cgtcggggcg caggacgagc atcagctcat aacggtgcaa ggcgaaactg
     1261 ctccttccca tgggatgtgc cccgcttggc ggcccggcgg gcaacggcgg aactgaaagg
     1321 ttgaccagaa gtatagccga gccgattgct agactcggtt cgggccagtc gcccgtcgtg
     1381 gaggtagccg ttgtcgtcgc acgtcgtcct ggtatcgctc ggcggtaacg cgcaggatct
     1441 cgcgagcgcg ctgtcgcgac tggacttgag cgtgtccgtc gcgcacgacg tgaatggggt
     1501 tttgctcgag cttgccgaca cgcagctgat cgtcgtcgag gctccggaca cggcttccct
     1561 ggccatgctc tgccggcgca tcaacgacga ggccgggtcg tcgcacccgc ccatccttgc
     1621 cgtggttgcc gcgagtgacg tcgagacgcg ggtgaaagtc ctccaggcgg gcgcagatga
     1681 cgtccttgcc cagcccatcg acgagcgcga gctggcggcc atggtggacg cactcctcct
     1741 gcgacctgcg ccggcaccgc cagggcgggc gccggcggcg tcatcctctc ccgcgcggcc
     1801 ccaggccacc ccgggccggg tgatcgtctt tgcgtcggcc aagggtggat cgggcacgac
     1861 cacgctcgcc gtgaataccg cgctggtgct ggcggagatg gcccccggca acgtcgcaat
     1921 tgccgacctg gacatgttcc acggccaggt ctcgacgcac ctcgacatct acggccgcgc
     1981 ctccacagcg accctggcgc gtgaggatct ggcaacgcag accccggaga tgatcacgga
     2041 ggcggggcgg cttcatccga gcggcctgat ggtctacggc ggaccctacc ggcccgacga
     2101 tgccgacgat ctgacggccg gccagctcac gtcgctggtc gcgacgctgc gcggcatgta
     2161 cgccaccgtc gtggtcgatg gtggctccac gctggaaagc cggcagatgg ccgtgctgca
     2221 tgctgcggac cgcctggcgc tggtggtcac gcccgacatt ccggctctgc gcctgctgca
     2281 tgcagcactc gaggtgctat ccgagatggg caatgctgcc gatcggacca ccttcgtggt
     2341 gaacgacatc taccccaagt cgtcgatctc ggccgaccag atcgaggagc acctgtcgat
     2401 ccgggttgga ctgacggtgc cctacgacaa cgagaacttc ctgcgtgcga tcaacgaagg
     2461 gcagccgatc gtcacgctct cgcggcgcac ccacgcggcc gcggcgattc gcaagctctc
     2521 ggaggtcctg gccgacaacg acaccgagga gggcgcaccg cggccgccga agaagggccg
     2581 cctgggaggc ctgctgggcc gatagggccg tcgtgctggg tgggttggtg cccgggatgg
     2641 gattcgaacc cacaagtcct ttcggacgga cgctcttaag gcgcctccgg ctaccaattt
     2701 cggcacccgg gctaacggag gatatcgctc gcgttgccaa tgttcctgcc tcggtaagtc
     2761 gctgtctgcg cgtggcagtt ggggcaaagg agcctgaggt tctcaatccg gttgtcctcg
     2821 cggcggccat tgacgtggtc caactcgagg gggatcggcg ccccattcca agtggtgttc
     2881 agacatgatt cgcagcgaga ccgcttaagc cctgcctcaa tcaggcgtcg tttgaggttc
     2941 gatgtctggc aaggtcggcc atcaacgaga agctggtcca gcggcgttgg cggtacgaca
     3001 ggcctcgtat cgccgaggcg ccatccggcg ccggtgaagt gggacgtgtc caaaccgagg
     3061 ctcaacaccc tggccttgag cgtcgtttgc gcgcgcccac cggaatagat cccgacccga
     3121 cggctcaagt cagcaaacga gcgcgactcg gacacggcct cgcgaatctg ctcgtccgta
     3181 gcccggtgca ccactcttcg cggaccgacc ctctgccctt ggagatgggg ggcgtagagg
     3241 ccgatcctgt cgatttggcg acggatggat tcgtagttac ccccgcgcgg gactaagccc
     3301 aaggccataa gaacgctgtg gaggtttccg ccggcggcga ccgcgctcac gagttgctcg
     3361 tcggtgtact ttcgtctgcg gtccatccac cgcgaacgta tcgctctgtc gtgccaactg
     3421 tgtggcacag cccgtgccga tagcggccgc atcgttgcag ggagtggagg cgacgaccgg
     3481 attcgaaccg gtgcatagcg gttttgcaga ccgccccctt aaccacttgg gtacgtcgcc
     3541 cctgaggctg gctggccctc ctggattcga accagggtta acggatccaa agtccgctgt
     3601 cctaccgcta gacgaagggc cagcgctaca cgcggcagtg tgacagggaa ggaatggagc
     3661 ggaagacggg actcgaaccc gcgaccctca ccttggcaag gtgatgctct accaactgag
     3721 ccacttccgc tcgatgacca ccggttgacg gaccggattg gtggtgccga gggccaggat
     3781 cgaactggcg acaccgcgat tttcagtcgc gtgctctacc gactgagcta cctcggcgcg
     3841 taagccctat cccggtcgcg gcaacgaccg ctcatattga accataacga cccgtcgcgc
     3901 cgcgtcggcg cggtcagtcg aggccggcca gcgcctggag cccgcgttcg tcgaccggtg
     3961 cctggccggt cagcgagcgc agccgattca cccggtcgat gatcggcgga tgcgtggccg
     4021 ttagaccggc cgcgcgagcc tcgaacttct tgatcggatt gacgaagtag aggtgctggg
     4081 tcgcccggtt ggcgacctcg aggacctcgc ggtcggagct gatcttgacc agcgcgcgct
     4141 cgaggccgta cgggttgcgc gtcagctcga cgctcgatga gtcggcgagg tactcgcgtt
     4201 ggcggctgac cgccagctga acgaggcggg caatgaatgg cgcaatgatc gacaggacga
     4261 gcccgaccac gatcgcgatg atcaccaggc cgcctccccc gcttccccga tcgcggctgc
     4321 cacggccgcc caagccgccc cagaaggtga agcgcaggaa gaagtcggcc agcagcgcga
     4381 tcgatccgac gagcgttgcc accaacagtg cgaatcggat atcgaggttg cgcacgtggg
     4441 ccagctcgtg gccgatcacc ccctgcagct cctcgcgatc gagcttctcg agcagcccgg
     4501 tcgtcaccgc caggctggcg tgcttggggt cgcgccccgt cgcgaacgca ttgggcgccg
     4561 tgtcgttgat cgcatatacc ttgggcacag ggacattcgc cgcgatcgac atctcgcgca
     4621 cgacgttcat gagctggggt gcaacctggt cgttgacctc gcgcgcgccc gacgcggcca
     4681 ggacgatgcc gtcacccgcg aagtacgaca cgctcgtcat cagcagcgcg atgactaccg
     4741 cgatgcccgt ggcaaccacg ccgccctgcc agtagccgcc gctcatcgcg aagccgatca
     4801 cgaacccgaa gaagcccagg aacgccgtca gcaggaaaac gagcagcagc gaccgccgcc
     4861 ggttggcgga gatctgcgag tagaaactct gaactggagc agtcatgtca tttgcccgcc
     4921 ggtcagccgg cgggcgtgat cagccgattc tgggcgattc agccgacctt cggcggggtg
     4981 ttgtaggtgc ccaggtcgac ctgcggcacc tgcgaggcct cgggctcggc atcgaagaac
     5041 tcgcgcttgc tgaagccgaa cgggccggcg atcagcacgt tggggaaggt cgcgatcgac
     5101 gtgttgtagt cgagcacggt cgcgttgtag tgctggcgcg cgaagccgat ctggttctcc
     5161 gtcgtggtca gctgctcttg cagctcgagc acgttctgat tggccttcag ctcggggtag
     5221 ttctcgacgg tggcgaagag tgagcgcagc gtgctggtca gcgcgttctc agcggcggcc
     5281 tgcgcggcag gaccctgggc gcccgcggtg accgcgttgg cgcgggcctc ggtcacctgg
     5341 gtcaagaccg actgctcgaa gcccatgtag cccttgaccg cgttgatcag gttggggatg
     5401 aggtcccagc ggcgcttcag ctggacttcg atctgggcga acgcctcatc gatccgcaga
     5461 cggcgctgga ccaggccgtt gtagatcagg atgccgccga cgatgacgac aacgatgagg
     5521 atcaggatga tgatcaagag tgtgtccatt aggtctccca cggttcttcg ccgcgtgacg
     5581 ggcagcgagg ttcgcataag tctattcgat ggggctgggc gcgccgttaa gatcgggtgg
     5641 tcgtggggga tcggcagccg ctgacagccg tgtcgcggaa acgaatcagc cgccgctctc
     5701 tgctgcgcct tggcgcagcg gcctcggtcg cgttcgccct gagcggccgc agggcgctgg
     5761 atgtcgccgc gtcgaccctc gctgggatgc ccggatcggg ttggctcgcg ggcacgactg
     5821 ccagcgcagt gccgcgcacg ctgtatcgcg ccgccgcgat tgccgacggc cgctccccag
     5881 cgctcgcccg ccagatgagc gtcctggtga gtggcggccg ggtggcgtgg atccgcccgg
     5941 cagacgcgga agaggatcca ggaccagcag atggcttgga gatcgtggac gcacgagggc
     6001 tgacgatcct gcccggactc gtcgatggcc actgccatct cacctccccc ggtggtcccg
     6061 actacatcgc gcacttcgag gacgcgccga gccagttgct gcgtactgcc gagcgcaacg
     6121 gccagctaag ccatgccgcc ggcgtggcct ggctgcgcga ggcgggtagc cccacggtga
     6181 ccgacccgac cgatggccgc cggcgcgcgt tggccctggg catccgcgac cgctgggcgg
     6241 gccgtaccga caggccccgg gttcgcgcgg caggcacgtg gatccaggtc aagggcggcc
     6301 tggcacgcaa cctgggtatc gtcgtcagca gcggcgacga gctcgtcgcg gccgtggagc
     6361 ggcagctcga tcagggtgcg gacctggtca agctttacgt cgccgcggac aactcgcgcc
     6421 aatcaccctg gacgccggca cagatcaacc gcgcggtcca ggccgcccat gcgcgcggcg
     6481 cccgtgtcgc ggcccacgca atgtggctcg gtcaggcgcg tgccgcggta gcaggcggag
     6541 tcgactcgat cgaacacggt ttccatctcg acgctgacct atgcgccgag atggtgcgcc
     6601 gcggcacgta cctcgtgacg acgctcagcg tgccgcgttc atggctgcgc atcggatcca
     6661 cgaccagcgg cacgtggtgg tccacgcgcg ccggccgccg cttcgcccgg a
//
LOCUS       DATBRX010000204         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3880583, whole genome shotgun sequence.
ACCESSION   DATBRX010000204 DATBRX010000000
VERSION     DATBRX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3880583"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VIF08_07935"
     CDS             <1..584
                     /locus_tag="VIF08_07935"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY8135952.1"
                     /translation="ADWLSTRWPDHEADYSRRWPDWRTVLVPLAGAAVGASLVSRWTE
                     PRDLAVLGVYCAALLVLLATDLDQKLLPDLITLPLIVLCGVALALNWAPALADKSFGQ
                     ASGLVAAIGLPAFLFVSDRLLRGDLGAGDLKLGVSVGLMSGVYNVFVGMIIASVAFSA
                     VLIVLILLRRLSLRSAVPFGPVLIVTGFIAALV"
     gene            719..1219
                     /locus_tag="VIF08_07940"
     CDS             719..1219
                     /locus_tag="VIF08_07940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8135953.1"
                     /translation="MLGLLALTAPLSVVTARSAPYMEWPTVGRETQPYGCTGFRMEPR
                     RGDCAHFHAGIDIANSRGTPVRAAADGTVAYVGREPWYHGTDRAWVVILSHGNGIKTI
                     YVHLQARPIDGIKKGKHVDQGQLIGLMGSTGRSTGPHLHFGVWLNRKGVDPVKYMPTS
                     KAPLHW"
     gene            1269..2636
                     /locus_tag="VIF08_07945"
     CDS             1269..2636
                     /locus_tag="VIF08_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY8135954.1"
                     /translation="MKRVTAVVLAGGEGERLSILSAVRAKPAVPFAGKYRIIDFTLSN
                     CVNSEIDNVLVLTQYNPRSLNDHIGAGRPWDLDRTKLGIRLLQPYVARGRASEWYRGT
                     ADAVLVNIEMIRQQPGDVVLILAGDHIYKMDYQPFVQFHRRNRADVTVAVQRVPIGEA
                     SRFGILALADDGRVTEWQEKPSQPKSDLASLGIYVFSKKALLAWLDDSRTDFGRDVIP
                     AMLDGGARVFGYEFDGYWQDVGTVAGYWQAHMDLLEEHPGLDLYDNEWLIHTRSEERS
                     PARVGPTASVHRSLISHGCQIWGTVERSVLSPGVRVDPGAVVRDSIVMFDTVIRAGAV
                     VDRAIVDKNVSIGPNAVVGFGSDFETANRQEPTRLNTGITVVGKRAVIPAGVRLGRNV
                     KVNEGARPADYGGKRVFASGSTIEVRRTPAGAAARGRATSARLRAEGEPLAGRMTAAQ
                     RGDAR"
     gene            complement(2887..3567)
                     /locus_tag="VIF08_07950"
     CDS             complement(2887..3567)
                     /locus_tag="VIF08_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135955.1"
                     /translation="MAKELRVLIEEPPKGKRWVAIAADWPGLERNGKSAADAVAKLTT
                     YLPRYLRVAGRAGLGPDFERQTKPTIIGQHTGTGSTDFWGISFAPSAEDHKPVADGEF
                     ERRLALLRAAWAEFDEVAARVSEELRPGVRGGGRSRDAIIRHVLVVEGSDFAARVKVP
                     TDFENPATRTLAHRKKHRERYVKAMRGWRRDDLPLGKWTLLYLLRHTAYHVLDHTWEM
                     QDRDLSAS"
BASE COUNT          536 a         1225 c         1247 g          620 t
ORIGIN      
        1 cggccgactg gctgtccacg cggtggcccg accacgaggc tgactacagc cggcgctggc
       61 ccgactggcg gaccgtgctc gtgccgctcg ccggcgcggc cgttggcgcc tccctggtga
      121 gtcgctggac ggagccacgc gacctggccg tcctcggcgt ctactgcgcc gcactgctgg
      181 tgctgctcgc gaccgacctc gatcagaagc tgctgcccga cctcatcacc ctgccgctca
      241 tcgtgctgtg cggggtcgcg ctggcgctca actgggcgcc ggctctcgcc gacaagtcgt
      301 tcggccaggc aagtgggctg gtcgccgcca tcggtttgcc tgcattcctc ttcgtgtcgg
      361 atcggttgtt gcggggagat ctcggcgcgg gtgatctcaa gctgggcgtg tcggtcgggc
      421 tgatgtcggg tgtctacaac gtgttcgtcg gcatgatcat cgccagcgtc gccttctcgg
      481 ccgtgctgat cgtgctcatc ctcttgcgcc gcctgtccct gcgctcggcg gtgccctttg
      541 gtccggtgct gatcgtgacc ggcttcatcg cggcactggt gtgaggttcg cgttgggtat
      601 ggacagtggc tttacgatgc gctttgcgaa ggaggccaac cccagtcggc ctcgggggat
      661 cgccgtggag agtgtcgtgt cttcgagatt tggtcgtcta gggctgagcg tgttgctggt
      721 gctcggactg ctggcgctga ctgcgccgct atccgtcgtc accgcgcgca gcgcgccgta
      781 catggagtgg ccgaccgttg gccgcgagac gcagccctac ggctgcaccg gcttcaggat
      841 ggagccgcga cgtggtgact gcgcgcactt ccacgccggc atcgacatcg ccaacagccg
      901 cggcacgccg gttcgtgccg ccgccgacgg cacggtcgcc tatgtcggcc gcgaaccgtg
      961 gtaccacggc accgaccgcg cctgggtggt catcctcagc catggcaatg gcatcaagac
     1021 gatctacgtt cacctccagg cgcgcccgat cgacggcatc aagaagggca agcatgtcga
     1081 ccagggccag ctcatcgggc tgatgggctc aacgggccgc tcgaccgggc cgcacctcca
     1141 cttcggcgtg tggctcaacc gcaagggcgt cgaccccgtc aagtacatgc ccacatccaa
     1201 ggccccgctc cactggtaag cgggcggctc acgtccgccc agcaattggg gggcgctagc
     1261 attcgcgcgt gaagcgcgtt accgcggtcg tgctcgccgg cggcgaaggc gagcgcctgt
     1321 cgatcctgag cgccgtccgc gcgaagcccg ccgtcccgtt cgcgggcaag taccggatca
     1381 tcgatttcac gctgtcgaac tgcgtcaaca gcgagatcga caacgtgctc gtgctgacgc
     1441 agtacaaccc gcgctcgctg aacgatcaca tcggcgccgg ccgaccgtgg gacctcgatc
     1501 ggaccaagct cggcattcgc ctgctccagc cgtacgtcgc tcgcggccgc gcgtcggagt
     1561 ggtatcgcgg caccgcggac gccgtgctgg tcaacattga gatgatccgc cagcaacctg
     1621 gtgacgtcgt cctcatcctc gccggcgatc acatctacaa gatggattac cagccgttcg
     1681 tccagttcca ccggcgcaac cgcgccgacg tgacggtcgc cgtccagcgc gtgccgattg
     1741 gtgaggcatc gcgcttcggc atcctggcgc tcgctgacga cgggcgcgtg accgagtggc
     1801 aggagaagcc atcgcagccc aagagcgacc tcgcgtcgct gggcatctac gtcttctcga
     1861 aaaaggcgct gctggcctgg ctcgacgatt cacgcaccga cttcgggcgg gacgtgatcc
     1921 cggccatgct cgacggcggc gcgcgcgtgt tcggctacga gttcgacggc tactggcagg
     1981 acgtcggcac cgtcgccggc tactggcagg cgcacatgga cctgctcgag gagcatcccg
     2041 ggctcgacct gtacgacaac gagtggctca tccatacgcg gtcagaggag cgctcgccgg
     2101 cgcgcgtcgg gccgaccgcg tccgtccacc gctcgctgat ctcgcacggt tgtcagatct
     2161 ggggcaccgt cgagcggagt gtgctgtcgc ccggagtgcg cgtcgatccg ggcgccgtgg
     2221 tgcgcgactc gatcgtgatg ttcgacacgg tcatccgcgc gggtgccgtg gttgaccggg
     2281 cgatcgtgga caagaacgta tcgatcgggc cgaatgcggt cgtgggcttt gggtccgact
     2341 ttgagaccgc caaccgccag gagccgacgc gtctcaacac cggcataacg gtcgtcggca
     2401 agcgcgcggt catccccgcg ggagtgcgcc tgggccgcaa cgtcaaggtc aacgaaggcg
     2461 cacggcccgc ggactacggc ggcaagcgcg tgttcgcctc cggatcaacg atcgaggtgc
     2521 gccgaacacc cgccggtgcc gcagccaggg gacgggccac gtccgcccgg ctgcgcgccg
     2581 agggtgagcc gctggccggg cgcatgaccg ccgcgcagcg cggagacgcg cgctagccac
     2641 aagtcgcggc ctgcttctgg tcagctgcgt gcacctggct cgatcggctc cctgtattcc
     2701 cgggcagatg acggcttggc aaccgctaaa caggctgtcg ccggccggcg ccttgtaggc
     2761 gtgcggggag agacaggaaa gtcggtcaga cggcaggctg acgtgaatct ggcggggaaa
     2821 cgcggtcctg cagctggtta agggtcgcca cgctgcagtt tgatcaggaa gtgggcgaga
     2881 tcgcggtcag gacgcggaca aatctcggtc ttgcatctcc catgtgtgat cgagcacgtg
     2941 gtaggccgtg tggcgcagga ggtacagcag ggtccatttg cccagcggca aatcgtcccg
     3001 gcgccaccca cgcatggcct tgacgtagcg ctcgcggtgc ttcttgcggt gcgcgagcgt
     3061 ccgcgtcgcc gggttctcga aatcggtcgg caccttgacg cgtgcggcga agtcgctgcc
     3121 ctcgaccacc aggacgtggc gaatgatcgc gtcgcgactt cggccgccgc cgcgcacgcc
     3181 gggccgcagc tcctctgaga cgcgcgccgc aacctcgtcg aactccgccc acgccgcgcg
     3241 cagcagcgcc agccggcgct cgaactcgcc gtccgcgacg ggcttgtggt cctccgcaga
     3301 cggggcgaag gagatgcccc agaaatcggt cgagcccgtg ccggtgtgct ggccgatgat
     3361 ggtgggcttg gtctggcgct cgaaatcggg gcccaggccc gcccgtcccg cgacccgcag
     3421 gtagcgcggc agatacgtgg tcagcttggc gactgcgtcg gcggccgact tgccgttgcg
     3481 ctcgaggccc ggccagtcgg ccgcgatcgc cacccagcgc ttgcccttgg gcggctcctc
     3541 gatcagaacg cgcagctctt tcgccatgcc gtcgaagata cttcaccgga tggcagcgca
     3601 tgttggggtg aaggtcgacg gctggctg
//
LOCUS       DATBRX010000205         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3545632, whole genome shotgun sequence.
ACCESSION   DATBRX010000205 DATBRX010000000
VERSION     DATBRX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3545632"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VIF08_07955"
     CDS             complement(<1..458)
                     /locus_tag="VIF08_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY8135956.1"
                     /translation="MPDQRFRGDPYAALDVRHEATDAQIKRRWRELAREHHPDRAAGD
                     ADAAATLTARMARINAAYDVLRDPVRRAAYDSSPAGRRSRAESGAAARGPLFGEDVPF
                     AGERGGPPPPPRTTPVTARFDTSAGLRPRNSRTGAPPATMRGMYPRARRDS"
     gene            542..1633
                     /locus_tag="VIF08_07960"
     CDS             542..1633
                     /locus_tag="VIF08_07960"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY8135957.1"
                     /translation="MKLPFEPPIEPMLAKLSPSLPTGDGWLFEPKWDGFRAIVFRDGD
                     ETYIQSRDLKPLDRYFPELGPALKANLPERCIFDGEIVIPGDDGLEFESLLLRIHPAA
                     SRVKMLAEQTPASFVGWDLLALDDRDLRATPQGERRALIEGQLRNVQPPIHLTPATRD
                     VELARDWFNRFEGAGLDGVIAKPLDAPYQPGKRVMLKIKHARTADCVVAGFRWHKNGP
                     GTHIGSLLLGLYDDKGDLHHVGITSSFTWDKRAALVSELEPLRKDALKHHPWAEWAEW
                     AGYGASDASGTRMPGATSRWNRGKDLSWEPLRIERVAEVAYDHLQGDRFRHATTFQRW
                     RPDKQPADCRYDQLEETAPFELAKIFGTD"
     gene            1788..2270
                     /locus_tag="VIF08_07965"
     CDS             1788..2270
                     /locus_tag="VIF08_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135958.1"
                     /translation="MDQQQQTPQWSPPPQQPMGWGGAGGMAPASRPLGVTLSAIWFYL
                     IGILLILAGALAIAVTSMFDSIFNAAGGAGFFAGLGVFAAVVVWIFAALFLLTGWGIW
                     KGRTWGRIGGIIWGVLGVLGGLGQLGARDGLVGGIIWIAIWAGVIYALWMAKAWFAAR
                     "
     gene            2343..>2974
                     /locus_tag="VIF08_07970"
     CDS             2343..>2974
                     /locus_tag="VIF08_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135959.1"
                     /translation="MTSDDEETKEHPVYPETPPPPPATPMAVDPLEPTTTINVAPPAP
                     PAPPAPPAPPSYPQPPNQGWQGPPPGAANLPGQWQSPGDAQPGYGAPGYPQAQTWGAA
                     APTYGTSAFVALAAMLLVIMGVVLAVLGAWLLTQGPALTDLVQRLRSVDLVVFKPTRD
                     QLRAVVSASPGTLMVFGVLQLLIGAFVLRHRSWARWLGILVALLGVLLSVV"
BASE COUNT          427 a         1041 c         1009 g          497 t
ORIGIN      
        1 gagtcgcgcc gcgccctggg gtacatgccg cgcatggtgg ccggcggcgc ccccgtcctg
       61 ctgttgcgcg gccgcaagcc ggcgctggtg tcgaagcgcg ccgtgaccgg cgtcgtccgc
      121 ggtggcggcg gcggcccgcc acgctcgccc gcgaacggca cgtcttcacc gaagagcggc
      181 ccacgagctg ccgctccgct ttctgccctt gagcgccgcc cggcgggcga gctgtcgtac
      241 gccgcgcggc ggaccgggtc gcggagcacg tcgtacgcgg cgttgatccg cgccatgcgc
      301 gcggtcagcg tggccgccgc atccgcatcg cccgcggcgc gatcggggtg gtgctcgcgc
      361 gcaagctcgc gccagcggcg cttgatctgc gcgtcggtgg cttcgtggcg aacatcgagc
      421 gcggcgtatg gatcgccgcg gaagcgctgg tcgggcatca accgctgacg ctaccacgcg
      481 cgccaccttg ggcggctgtt ccggccgccg cctgtacgac caccgcctat agtcggtgcc
      541 cgtgaagctc cccttcgagc ccccgatcga gccgatgctc gccaagcttt caccatctct
      601 gcccacgggc gacggctggc tcttcgaacc caagtgggac ggctttcgcg cgatcgtgtt
      661 tcgcgacggc gacgagacgt acatccagag ccgcgacctc aagccgctcg accgttactt
      721 cccggagctg ggcccggcgc tgaaagccaa cctgcccgag cgctgcatct tcgatggcga
      781 gatcgtcatc ccgggcgacg acgggcttga gttcgagtcg ctcctgttgc gcatccatcc
      841 cgcggcgtca cgcgtcaaga tgctggccga gcagacgccg gcgagcttcg tcggctggga
      901 tctgctggca ctcgacgaca gggacctccg cgcgactccc cagggtgagc ggcgcgccct
      961 catcgagggg cagctcagga atgtccagcc gcccatccac ctcacgccgg cgacgcgcga
     1021 cgtcgagctc gcgcgcgatt ggttcaaccg cttcgagggc gccggcctcg acggcgtgat
     1081 cgcgaagccg ctcgatgcgc cctatcagcc gggcaaacgc gtgatgctca agatcaagca
     1141 cgcccggacg gccgactgcg tcgtcgccgg cttccgctgg cacaagaacg gcccggggac
     1201 gcacattgga tcgctgctgc tcgggctgta cgacgacaag ggcgacctgc atcacgtcgg
     1261 gatcacctcg tcgttcacct gggacaagcg tgcggcactc gtttctgagc tcgagccttt
     1321 gcgcaaggac gcgctgaagc atcatccgtg ggccgagtgg gcggaatggg ccggctacgg
     1381 cgcgtccgat gcgagcggca cgcgcatgcc cggcgcgaca agccgctgga accgcggcaa
     1441 ggatctgtca tgggagccac tgcgcatcga gcgcgtcgcc gaggtcgcat acgaccatct
     1501 ccagggcgat cgcttccgcc acgcgacgac cttccagcgc tggcgtcccg acaagcagcc
     1561 cgcagactgc cgttacgacc agctcgagga aaccgccccg ttcgagctcg ccaagatttt
     1621 cggcaccgac tgaccgaccc ggttggccaa tctccaacac gtcgaaatcg gcgcgcgggt
     1681 gctcgtcggc gctcgtaaac tcggcctgtt cgtatttgct cacccgccgg cgcgcatgcg
     1741 cccatcgccc atgagcgggt agcgacatag cgcagggagg gtagtacgtg gatcagcagc
     1801 agcaaacgcc gcagtggtca ccgccgccgc agcagccgat gggctggggc ggcgcgggcg
     1861 gaatggcccc ggcgtcccga ccgctcggcg tgacgctgag cgcgatctgg ttctatttga
     1921 tcggcattct gctgatcttg gccggagccc tggccatcgc ggttacgagc atgttcgatt
     1981 ccatctttaa cgccgccggc ggtgccggct tcttcgccgg cctcggcgtc ttcgccgctg
     2041 tagttgtttg gatcttcgcg gcgctgttcc tgctgactgg ctggggcatt tggaagggcc
     2101 gaacgtgggg ccggatcggc ggcatcatct ggggtgtgct cggcgttctc ggtggcctcg
     2161 gccaactggg cgcgcgcgac ggcctggtgg gcggcatcat ctggatcgcc atctgggcag
     2221 gcgtcatcta tgccctctgg atggccaagg cctggttcgc cgctcgctga gggcggatac
     2281 ttccgcatag gaagcgccgg ccgtccgcaa ggtcggccgg cgttgttgtt tttggagcgg
     2341 tcatgacgag cgacgacgag gaaaccaagg aacacccggt ctatccggag acgccgcctc
     2401 cgccgccggc tacaccgatg gcggtcgatc ccctggagcc gaccacgact atcaacgtcg
     2461 cgccaccggc accaccggca ccaccggccc cacctgcgcc accgagctac ccgcagccgc
     2521 cgaaccaagg ctggcaaggt ccgccaccgg gagccgcgaa tctgcccggt cagtggcagt
     2581 caccgggcga tgcccagccc ggttacggcg cgcccggcta cccgcaagcg caaacctggg
     2641 gcgcggccgc gcccacctat ggcacctctg cgttcgtagc gctcgcggca atgttgctcg
     2701 tgatcatggg cgtcgtcctc gcggttctgg gggcatggct cctgacgcag ggtccggcgc
     2761 tgaccgatct cgtgcagcgc ctgcgcagcg ttgatctggt cgtcttcaag ccgacgcgcg
     2821 atcagctccg cgcggtcgtc agcgcgtcgc cgggcacact catggtgttc ggtgtgctgc
     2881 agctgttgat cggtgccttc gtgctgaggc accgcagctg ggcgcgttgg ctgggcatcc
     2941 tcgttgcact gctgggcgtg ctgctcagcg tcgt
//
LOCUS       DATBRX010000206        11166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2541623, whole genome shotgun sequence.
ACCESSION   DATBRX010000206 DATBRX010000000
VERSION     DATBRX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11166
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2541623"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VIF08_07975"
     CDS             complement(<1..578)
                     /locus_tag="VIF08_07975"
                     /inference="COORDINATES: protein motif:HMM:NF023853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEY8135960.1"
                     /translation="MVLDLDQQSVGVSHGATEGGANGSGRSAPRGGGAAGPSTGSGMT
                     ARQVATSAISNTRVESTQDFLSHAGADIYGQYVFGDEAQRQYLAKPIAAKLRRTTAGL
                     EPFDPQIVDAVAHGVKEWALAHGATHYTHWFVPMTGSTAEKHDSFLVPTGDAKTIAEF
                     SGKNLVQGEPDASSFPSGGIRATFEARGYTAW"
     gene            complement(655..2127)
                     /locus_tag="VIF08_07980"
     CDS             complement(655..2127)
                     /locus_tag="VIF08_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY8135961.1"
                     /translation="MALNPLGRLGWLAWHTLTSVKFAVLQISILAVAGVFGAVLRQLP
                     AFALHDPAAYADEMAQIHSAYDPLSVLGLNIGPGMVDLFERLGLFRVFSAPWFVFLLT
                     LLVVSIVVCTLDRLPDLWRTAHRVRIAQAAPFFDLRLDHRAAYADAGTGGAEALREVL
                     RGGRYRVREEVADEKDGAAGVLHLYGDKNQYLKLATLFTHLGLILFLAGAAVTTAFGY
                     ETVVFLGEGQTAPVRAVGTPDNLLVKNIHFEAPTRPDGSFADFRTDLAVYRNGVQVAR
                     KIIRVNDPLEVDGYVFHQNTFGPAENLSIWDANGALVWDGPVLLDGTLAGLPQGFLTI
                     PGSQMGLLVVLNRATDGTALLALSGITADELPNGGNVCFLQVLGLGATSEPGTTCGYT
                     IGWASAGAYTGMVIKRDPGQWFIWIAYASLIVGLALTFYFPRRRVWARLDGERLELAM
                     AADRYVDVEREFGQLQDELSARLRNRPESRLQPNSSSGRP"
     gene            complement(2157..3377)
                     /locus_tag="VIF08_07985"
     CDS             complement(2157..3377)
                     /locus_tag="VIF08_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8135962.1"
                     /translation="MSEEGQRPGLGFGTRALRAATTPPQVDQKPDSVPIYNAVTYSTE
                     SAAELADVLGDTKPGYAYSRIDNPTATALSTTLAEIEGAESAYVFASGMAAIHATFVA
                     LLKQGDHVVAARQIYGSVQHLLDKILPRFGIETTFVDATDLDAVAAAFRPSTRLLHVE
                     TIANPTIVVSDLAALIELGHSRGTTVSVDNTFASPWLCRPAELGADLVIEALTKWIGG
                     HSDVLAGGVSGRADLIAAIRRVQIDTGGTIAPMSAFLVLRGIETLHVRMERHSANALA
                     LARHLEGAGLNTVSYPGLPSHPQFAVAQRQLRVGGGMMAFELADRATAERFLDALALP
                     PRTASLGSVRTIAVHPPSSTHRQLDSAELAAIGIPDGLVRVSVGLEEIDDLIADFDQA
                     LAAAQARAAAGIGT"
     gene            complement(3390..4829)
                     /locus_tag="VIF08_07990"
     CDS             complement(3390..4829)
                     /locus_tag="VIF08_07990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8135963.1"
                     /translation="MPIDQLALLFLVVIAANLLVLLALQFAWRGRRSQTEPDRQPALT
                     QGDGAADGSLRLLTSASALPPQLYQRVVRVASLVFILAAMVDVQLTATGPVTAIMVFL
                     AVGAIAVVLSQEILPAGRLGRWRLPIEAGVAIIFVTGLLVLTGGNTSPFFFGYILLVG
                     AAALWASEFGALLLAVATSLAYLIGVLVPLNSAPSTSADIGRIAFNLIALALVAYVCA
                     IVGREQRRAREEALRLSRYDSLTGLHSRDFFLAEMEQEILRAGRSGRPFALLMLDLDG
                     LKAANDRFGHASGDTLLRAVADTLRGDIRITDVAARWGGDEFVLLLPETDLNGAMLVA
                     DKVRISIGRLALPHDGLVVRTSASIGLVTYPDDGRSTTELMRRADLAMYEAKRRGRDQ
                     VVRFARAAKPESGPAATKPIGVVDASVATGDGATGPLTGMPAPAVVVTAEGGSVPAAP
                     VPTAPAAPPERPAVPSPWVGRAPWESTGN"
     gene            5051..6298
                     /locus_tag="VIF08_07995"
     CDS             5051..6298
                     /locus_tag="VIF08_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8135964.1"
                     /translation="MLRPALAVDRNGRIVLLARTLRSFAFGLNSVALGLYLAGIGLDG
                     GQIGLVLSAALAGTLGLTLVIALYGDRIGRKRLLMAGSALMMAAALVPLLGSQPLLLA
                     AIALSGMVAVNANESTGLQTVDQALLPQAVPPEQRTAAFALYNLLAALAAALGALAIG
                     LFPAIGAALGLSGANIYLPAFVLYGLIGLVCLVIQSRLDARAETGERIQKRLAIDQSR
                     GIVRRMSILYGLDSFAGSFTVQSFIAYFFVAAFGAEPAAVGALLFGANLLAALSFPVA
                     AWLSRRIGLINTMVFTHIPSSLFLFGVALAPSFAIAAPLWLARAALSSMDVPTRQSYT
                     MAVVEPAERTAAAGITSLARAIALVPGPAIAGALLVPLGLAVPLIATGVLKIAYDLSL
                     FALFRTHPAPEERLRRAAQEPKA"
     gene            complement(6295..7179)
                     /gene="efeU"
                     /locus_tag="VIF08_08000"
     CDS             complement(6295..7179)
                     /gene="efeU"
                     /locus_tag="VIF08_08000"
                     /inference="COORDINATES: protein motif:HMM:NF041756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEY8135965.1"
                     /translation="MELGSLIAGLMTGLREGVEAALIVSIILAYLSKTGNRRHFSKIW
                     MGTAAAVVVALVIGIGLFVTVGGFEEPYEQLFEASTMILATAVLTWMLFWMRRQARSV
                     KGELQAAVDRVLTEGSAWGLALLAFTAVIREGVETSLFLVGQVNAAAAEGAGAELGTL
                     LGALIGLAIAVAIGWMIYTGARRINYGTFFRLTGLVLVFIAAGLLSHAVHELIEVSWI
                     TVLTGTAFDISAVLPDDAGVGLFLRALFGYAAAPEVLTLIVYLAYLVPVLILYLRPVP
                     VAPAQPQAAAAPTTPAPS"
     gene            complement(7289..8626)
                     /locus_tag="VIF08_08005"
     CDS             complement(7289..8626)
                     /locus_tag="VIF08_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135966.1"
                     /translation="MLSRLGEPCRRPIKWVLLLATLLGLLGLAPVPTAAAADAPNSLG
                     LTATYDINAMIHWNANRLSVTSVATVTNTTENVVEAMTFNFIPARIGNLALNEVRVGE
                     EGANAQIDDANLIVSMPVPLDPGAQTTVTIVYDATFNTGSKNKKWLFSKIDNVVTAYR
                     WIPWLSKRHRWSTPNFGETYVTQVASDVRVTLSSDRPGVKYATTGHQTEGDGMTQVFV
                     AHNVRDFNFSASPRYKVSTSDVNGVTLSTYTFSLKASALSGYTRTAFNEFSDKVGPYP
                     YETLDVAEIPRGGGMESPGMLWIPSNATSGSRKRYLVTHEVAHQWFYSVVGNDQATDP
                     FADEAVAEFLVRTIIGFRSSKCGETRLDGSVYDYGSRCYYEVIYVQGADYLNEYRDRV
                     GGDAFWAGLRAYYERFRFGLGGNYELLQTLDEQAGESGGGHEARFPSLYPPES"
     gene            complement(8759..10000)
                     /locus_tag="VIF08_08010"
     CDS             complement(8759..10000)
                     /locus_tag="VIF08_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135967.1"
                     /translation="MPAVDAAPNDSIGLSATYDVSANLQWSNRRLVVNSTATVKNTSS
                     SNVTGLTFNLVPAKIGRINMNGVTVNGSAASTTVDDMNLIVHLNNALAPNASADVTIR
                     YSATFNSTNPNRKWLFALRHGIVTAYRWIPWLSNRYPFKTPLFGDAFTTQVASRVDVS
                     ITADRGGLIYAAPGTRTSVNGNTQNFVAYNVRDWNFSASPDYHVGTEMHGGVQFEYYT
                     INQSQSAFARYAKQAFDKYVPLVGAYPYSKLFIAETSPGFAMESPQGFWIPDSYAPKD
                     VQFAVIHEVAHAWFYGAVGSDQATQPFADEAVAEMLTRLIAGNRGAHCANDNLDGSVY
                     SYTSDCYYEVIYIQGTNYLNAYRRRVGDANFWQGMRAYIAQYKWKMGGTKQLLTTLDA
                     HSGGKGGGHHARFPSLFPAGS"
     gene            complement(10156..10713)
                     /locus_tag="VIF08_08015"
     CDS             complement(10156..10713)
                     /locus_tag="VIF08_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY8135968.1"
                     /translation="MGLLTFSYNITLDGCVDHREGIADDETHAFYTGLLDEAGAMLWG
                     RVTYEMMESYWPAVARGDEEAPSAMREWAIKLEAKPKYVVSATRADFPWNNSHHIAGD
                     LRDAVQKLKDATPAGVLLGSGKLATELDRLDLIDEYRLLVHPRIAGHGPTLYQGGLPA
                     TRELELMSAKPLRNGAVAMHYRRAR"
     gene            complement(10760..10836)
                     /locus_tag="VIF08_08020"
     tRNA            complement(10760..10836)
                     /locus_tag="VIF08_08020"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10800..10802),aa:Pro,seq:tgg)
     gene            10888..>11166
                     /locus_tag="VIF08_08025"
     CDS             10888..>11166
                     /locus_tag="VIF08_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY8135969.1"
                     /translation="MEAFALSFGVILLAELGDKSQLVTIAFTARYRPVVVLAGIATAA
                     ALINGLAVVIGVAFAAMLPLHVLQAIGGVLFLAFAAMTLWRTEGDDEEL"
BASE COUNT         1780 a         3718 c         3831 g         1837 t
ORIGIN      
        1 tcccacgcgg tgtagccgcg cgcctcgaag gtggcacgaa tgccaccgct cgggaaggac
       61 gacgcgtccg gctcgccctg gacgaggttc ttgccactga actcggcgat ggtcttggcg
      121 tcgcccgtcg gcacgaggaa cgaatcgtgc ttctcggcgg tggagccggt catcggcacg
      181 aaccagtgcg tgtagtgcgt cgcgccgtgg gccagtgccc actccttcac accgtgcgcc
      241 acggcatcga cgatctgcgg gtcgaacggc tcgagtccgg ccgtggtgcg gcgcagcttg
      301 gcggcgatgg gcttggcaag gtactggcgc tgcgcctcgt cgccgaacac gtactggccg
      361 tagatgtcgg cgccggcatg gctgaggaag tcctgggtcg actcgacccg cgtgtttgag
      421 atcgcgctgg tggcgacctg acgggcggtc atgccgctgc cggttgatgg gcctgcggcg
      481 ccaccgccac gcggcgcaga gcggccgctg ccatttgcgc cgccttcagt ggcgccgtgg
      541 ctgacgccga cgctctgctg gtccaggtcg agcaccatcg tcgaaagtcc ctccctcgtc
      601 ccgcgcacgg tcgcagccga ccacccagcc gacactgcac ggctggcacc gagtctacgg
      661 ccgtccggag ctggaattcg gttgcaaacg ggactcgggc cggttacgta gtcgagccga
      721 cagctcgtcc tggagctggc cgaactcgcg ctccacgtcc acgtatcggt cggccgccat
      781 ggccaactcg agccgctccc cgtcaaggcg cgcccagacg cgccggcgcg ggaagtagaa
      841 ggtcagggcg aggcccacga tcagcgacgc ataggcgatc cagatgaacc actgccccgg
      901 gtcgcgcttt atgaccatgc cggtgtaggc gccggccgac gcccagccga tggtgtagcc
      961 gcacgtcgtg cccggctcgc tcgtcgcgcc caggcccagc acctgcagaa agcacacgtt
     1021 gcccccgttg ggcaactcat cggcggtgat accgctcagc gccaggagcg ccgtgccgtc
     1081 agtagcgcgg ttgagcacca ccagcaggcc catctgcgaa ccggggattg tcaggaagcc
     1141 ctgcggcagg ccggccaggg tgccgtcgag cagcaccggc ccatcccaga ccagcgcgcc
     1201 gttggcatcc cagatcgaca ggttctcggc cgggccaaag gtgttctggt ggaagacgta
     1261 gccatcgacc tcgagggggt cgttgacgcg aatgatcttg cgcgccacct gcacaccgtt
     1321 gcggtagacc gcgaggtcgg tccggaagtc ggcaaacgag ccgtccggtc gggtgggagc
     1381 ctcgaagtgg atgttcttga ccagcaggtt gtcgggggta ccgactgccc gcacgggtgc
     1441 cgtctgacct tcccccagga acaccacggt ctcgtagccg aacgcggtgg tcaccgctgc
     1501 gccggccagg aacaggatca ggccgaggtg ggtgaagagc gtggccagct tcaggtactg
     1561 gttcttgtcg ccgtacaggt gcagcactcc tgccgcgccg tccttttcgt cggcgacctc
     1621 ctcgcgcacg cggtagcgcc cgccgcgcag cacctccctg agcgcctcgg caccgcccgt
     1681 gcccgcatcg gcataggcgg cgcggtggtc gaggcgcaaa tcgaagaagg gcgcggcctg
     1741 ggctatgcgg acgcggtgcg cggtgcgcca caggtcgggc agccgatcga gcgtgcacac
     1801 gacgatgctc acgacgagca gcgtcaggag gaacacgaac cacggcgcgc tgaagacgcg
     1861 gaacaggccc agccgctcga agagatcgac catgcccggc ccgatgttga gcccgagcac
     1921 gctgagcggg tcgtacgcgg aatggatctg ggccatctcg tcggcgtacg cggccggatc
     1981 gtgcaacgcg aacgccggta gctggcgcag caccgcgccg aacacgccgg ccaccgcgag
     2041 gatgctgatc tgcagcacgg cgaacttgac gctggtcagc gtgtgccagg cgagccagcc
     2101 gagccggccg aggggattga gcgccatggc gcgtctcgcg cccagagtcg tgcttgctat
     2161 gtccctattc ccgccgcggc acgcgcctgg gcggccgcga gtgcctggtc gaagtcggca
     2221 atgaggtcgt cgatctcctc cagtccgact gagacgcgca ccaggccgtc ggggatcccg
     2281 atcgccgcga gctcagccga gtccagctgg cggtgcgtgc tcgacggcgg gtggaccgcg
     2341 atggtgcgga cgctgcccag ggaagcagtg cgcggcggca gcgcgagcgc gtcgaggaac
     2401 cgctcagcgg tcgcgcgatc ggccagctcg aaagccatca tgccgccgcc gacgcgtaac
     2461 tgacgttggg caactgcgaa ctggggatgt gacggcaacc cggggtagct gaccgtgttg
     2521 agccccgcgc cctccaggtg gcgcgcgagt gcgagagcgt tggcgctgtg gcgctccatg
     2581 cgcacgtgga gcgtctcgat tccgcgcagc accaggaagg cgctcatcgg cgcgatcgtg
     2641 ccaccggtgt cgatctggac gcgccggatg gcggcgatca ggtcggcgcg gcccgacacg
     2701 ccgcccgcaa gcacgtcgga atggccgccg atccacttgg tgagcgcctc gatcaccagg
     2761 tcggcgccca actcggccgg gcggcacagc cacggtgacg cgaacgtgtt gtcgacgctg
     2821 accgtggtgc cgcggctgtg gcccagctcg atcagcgccg cgagatcgga aacgacgatt
     2881 gtcgggttgg cgatcgtctc gacgtgcagc agccgcgtgc tgggccggaa ggccgccgcg
     2941 accgcgtcaa gatccgtggc gtcgacgaac gtcgtctcga tcccaaagcg cggcaggatc
     3001 ttgtcgagca gatgctgcac gctgccatag atctggcgcg cagcgacgac atggtcgccc
     3061 tgcttcagca gtgccacgaa cgtggcgtgg atcgcggcca tgccgctggc gaagacgtag
     3121 gccgattccg cgccctcgat ctcggcgagc gtcgtggata gtgccgtggc cgtgggattg
     3181 tcgatacgcg agtaggcata gccgggcttg gtgtcgccca ggacgtcggc cagctcggca
     3241 gctgattcgg tcgagtaggt gacggcgttg tagatcggta cggagtcggg tttctggtcg
     3301 acctgcggcg gggtcgtggc ggcgcgcagc gcgcgggtgc caaagcccag gccaggccgc
     3361 tggccctctt cactcatgga gcgatgctac tagttgccgg tcgactccca tggcgcgcgg
     3421 cccacccagg gtgagggcac ggcgggccgc tcgggtggcg cggcgggagc ggttggaacg
     3481 ggcgccgccg gcaccgatcc accctccgcg gtcacaacca ccgccggcgc aggcatgccc
     3541 gtcagcggac cggtcgcgcc gtcgcccgtc gcgaccgacg cgtcgacaac gccgatcggc
     3601 ttcgtcgcag ccggtccgga ctccggcttg gctgcgcgcg cgaagcgcac tacctggtcg
     3661 cgtccgcgcc gcttggcttc gtacatggcg aggtcggcac ggcgcatgag ctcggtcgtt
     3721 gaccggccgt catcggggta ggtgactagg ccgatcgaag ccgacgtgcg cacaaccagc
     3781 ccgtcgtgcg gcagcgccag ccggccaatg gaaatgcgca ccttatcggc gaccagcatc
     3841 gccccgttga gatccgtttc tggcagtagc aggacgaact cgtcgccacc ccagcgcgcg
     3901 gccacgtcag tgatgcggat gtcgccacgc agcgtgtcgg caaccgcccg aagcagggtg
     3961 tcgcccgatg catggccaaa gcgatcgttc gccgccttaa gcccgtcgag gtcgagcatc
     4021 agcagcgcga atggccggcc gctgcgcccg gcgcgcagga tctcctgttc catctcagcg
     4081 aggaagaagt cgcgactgtg gagtccggtc aatgagtcat agcgcgacag gcgcagcgcc
     4141 tcctctcgcg cgcggcgctg ctcgcggccg acgatcgcgc agacgtaggc gacgagagcg
     4201 agagcgatga ggttgaacgc gatccggccg atatcagcgg aggtcgaggg cgcgctgttg
     4261 agtggcacga gcacgccgat cagataggcc aggctcgttg ccactgccag gagcagtgcc
     4321 ccaaactcgg atgcccacag cgcagccgcg cccacgagca ggatgtagcc gaagaagaac
     4381 ggacttgtgt tgccaccggt gagcaccagc agcccggtca cgaaaatgat ggccacgcca
     4441 gcctcgatcg gcaggcgcca ccggccaagc cgaccagccg gcaggatttc ctgcgacagc
     4501 accaccgcga tcgcgccaac ggccagaaac accatgatcg ccgtgaccgg cccggtcgcc
     4561 gttagctgga catcgaccat cgccgcgaga atgaaaacga ggctggccac gcggacgacg
     4621 cgctggtaga gctgcggcgg cagggcggac gcgctcgtca gcagacgcag cgaaccatcg
     4681 gctgccccgt cgccctgcgt caaagcgggc tgccggtcag gctcggtctg gctgcggcgg
     4741 ccgcgccacg cgaactggag ggcgagcagc acgagcagat tcgccgcgat gacgacgaga
     4801 aacagcaggg cgagctggtc gattggcacc ttgaacagac gacgggtcgc ccggtatggc
     4861 cgagggaggg cgagatggtc cctctatgcg ctgtacccga tggtaccgcg acgtataggc
     4921 cggaagtact accgactggt cgtttagtgt ggcgccgatg caccgcaagc ccgattcccg
     4981 cgaaacgcct gcctccgcag acgccccgcc gggcgctgct gcgcgctccg gtcgaagccc
     5041 cgctcgctcg gtgcttcggc ctgcgcttgc ggtcgaccgc aacggtcgga tcgtacttct
     5101 ggcgcgcact ctgcgcagtt ttgccttcgg cctgaactcg gtcgcgctgg gcctgtacct
     5161 ggccggaatc gggctcgacg ggggccagat cggactggtc ctgtcggcgg ctctcgccgg
     5221 caccctgggc ctgacgctgg tcatcgcgct gtacggcgat cgcatcgggc gcaagcggct
     5281 gctgatggca ggctcggcgc tgatgatggc cgcggcgctg gtgccgctcc tcggcagcca
     5341 gccgctgctg ctcgccgcga tcgcgctcag cggcatggtc gcggtcaatg ccaacgagtc
     5401 gaccggcctc cagacagtcg accaggcgct cctgccgcag gccgtgccgc ccgagcagcg
     5461 gaccgcagcc ttcgcgctct acaacctgct cgccgcgctc gcggccgcgc tcggtgcgct
     5521 ggcgatcggc ctattcccgg ccatcggcgc cgcgctcggg ttgagcggcg caaacatcta
     5581 cctgccggcc ttcgtcctgt acgggctgat cggcctcgtg tgcctcgtca tccagtcgcg
     5641 cctggacgcg cgcgcggaga ccggcgagcg gatccagaag aggctcgcaa tcgaccagtc
     5701 gcgcggcatc gtcaggcgca tgtccatcct ctacggcctc gactcgttcg ccgggtcgtt
     5761 cacggtgcag agcttcatcg cctacttctt cgtggccgcg ttcggggctg aaccggccgc
     5821 cgttggcgcg ctgctgttcg gagccaacct gctcgcggcg ctgtccttcc cggtggcggc
     5881 gtggctgtcg cgccggatcg ggctcatcaa cacgatggtc ttcacccaca tcccatcgag
     5941 cctcttcctg ttcggcgtcg ccctggcgcc gagcttcgcc atcgccgctc ccctgtggct
     6001 tgcccgggcg gcgctgtcat ccatggacgt gccgacgcgc cagtcgtaca cgatggccgt
     6061 ggtcgagccg gcggagcgca cggccgcggc gggcatcacc agccttgccc gcgcgattgc
     6121 tctggtgccc gggccggcca tcgccggcgc gctgctcgtg ccgcttgggc tggcagtgcc
     6181 gctgatcgcg acgggcgtcc tgaagatcgc ctacgaccta tcgctgttcg cgctgttccg
     6241 gacgcacccc gcgccggagg agcggttgcg ccgcgcggcg caggagccga aggcctagga
     6301 cggcgccggg gtcgtgggcg ccgcggccgc ctgtggctgc gccggcgcga ccgggaccgg
     6361 ccgcaggtac aggatgagca ccggcaccag gtaggcgaga tacacgatca gcgtcagcac
     6421 ctccggcgcg gccgcatagc cgaacagcgc gcgcaggaac aggcccacgc ccgcgtcgtc
     6481 gggcagcacc gcgctgatgt cgaacgcagt cccggtcagg acggtgatcc agctgacctc
     6541 gatcagctcg tgcacggcgt ggctgagcag gccggcggca atgaagacga gcaccaggcc
     6601 ggtgagtcgg aagaaggtgc cgtagttgat ccgccgcgcg ccggtgtaga tcatccagcc
     6661 gatcgcgacc gcgatcgcca ggccaatgag cgcgccgagc agcgtgccga gctcggcacc
     6721 cgcgccctcc gcggccgccg cgttcacctg gccgaccagg aacagcgacg tctccacgcc
     6781 ttcgcgaatg acggcagtga atgcgaggag cgccagtccc cacgcgctgc cctctgtcag
     6841 gacgcgatca acagcggcct gtagctcgcc cttcacggag cgcgcctggc ggcgcatcca
     6901 gaaaagcatc cacgtcagaa cagcggtggc gaggatcatc gtgctcgcct cgaacagctg
     6961 ctcgtacggc tcctcgaacc cacccaccgt gacgaagagc ccgatgccga tcacgagcgc
     7021 gaccacgacc gcagcggccg tgcccatcca gatcttgctg aagtggcgcc gattgccagt
     7081 cttggagagg tacgccagga tgatgctcac gatcagcgcc gcctcgacgc cctcgcgcag
     7141 tccggtcatc aggccggcga tcagtgagcc cagttccacc cgccacctca gcgttaatcc
     7201 cgactgatcc agtcgggatt gccatgctaa atgggcgggc gcggagcgtc aatccaagcg
     7261 ccacgagccg tgcgggaagg agcctgcctc aggactcggg cgggtagaga ctcgggaagc
     7321 gcgcctcgtg gccgccgccg ctttcgccgg cctgctcatc gagcgtctgg agcagctcgt
     7381 aattgccgcc caggccaaag cggaacctct cgtaataggc gcgcaggccg gcccagaagg
     7441 catcaccacc gactcggtcg cggtattcgt tcaggtagtc ggccccctgg acgtagatga
     7501 cttcgtagta gcagcgcgaa ccgtagtcgt agacgctgcc gtcgagcctc gtctcgccgc
     7561 acttggagct gcgaaagccg atgatcgtgc gcaccaggaa ctctgccacc gcttcgtcgg
     7621 cgaatggatc cgtcgcctgg tcgttgccga ccaccgaata gaaccactgg tgcgccactt
     7681 catgagtgac gaggtagcgc ttgcgcgatc cgctcgtggc attgctcggg atccagagca
     7741 tgcccggtga ctccatgcca ccaccgcgcg ggatctcggc gacgtcgagc gtctcgtacg
     7801 ggtagggacc caccttgtcg ctgaactcgt tgaacgccgt ccgcgtgtaa ccactgagcg
     7861 cgctggcttt cagcgagaac gtgtaggtgg acagcgtcac gccgttgaca tcgctcgtgg
     7921 acaccttgta gcgcgggctc gcgctgaagt tgaagtcgcg cacgttgtga gcgacgaaga
     7981 cctgggtcat gccgtcgccc tcggtctggt ggccggtggt ggcgtacttc acgccggggc
     8041 gatcgctgga cagcgtcact cggacgtcgc tcgctacctg ggtgacgtac gtctcgccaa
     8101 agttgggcgt cgaccagcgg tggcgcttgc tcagccacgg gatccagcgg taagcggtaa
     8161 ctacgttgtc gatcttcgag aagagccact tcttgttctt ggagcccgtg ttgaacgtcg
     8221 cgtcgtagac gatcgtgacc gtcgtctgcg cgcccgggtc gagcggcacg ggcatgctca
     8281 cgattaggtt ggcgtcatcg atctgagcgt tcgcgccctc ctcgccaacg cgcacctcgt
     8341 tcagcgcgag gtttccaatg cgcgccggga tgaagttgaa cgtcatcgcc tcgacgacgt
     8401 tctcggtcgt gttggtcacc gtcgcgacgc tggtgacgct cagccgattc gcgttccaat
     8461 ggatcatcgc gttgatgtcg taggtcgcgg tcaagcccag gctgttcggg gcatcagcag
     8521 ccgccgctgt tggcacgggc gcaaggccca gtaacccaag cagcgtcgca agcagcagta
     8581 cccacttgat cgggcggcga cacggctcgc ccagccgact cagcacgccc gaagcatgac
     8641 cgaaccgact ggctgctgtg aaccgtactt gtcaaccaaa acgactgata ctgacggcgt
     8701 tgcgcgatct gaaagcccgg tgcaaccaaa ctgggcacga cgtgccccca gttcgcgatc
     8761 agctcccagc tgggaagagg ctcgggaagc gcgcgtgatg gccgccaccc ttgccgccag
     8821 agtgggcgtc gagcgtcgtc agcagctgct tggtgccgcc catcttccac ttgtactgcg
     8881 cgatgtaggc acgcatgccc tgccagaaat tcgcgtcacc gacgcgcctg cggtaggcat
     8941 tgaggtagtt ggtgccctgg atgtagatga cctcgtagta gcaatcggag gtgtacgagt
     9001 agacggaacc atcgaggttg tcgttcgcgc aatgggcgcc gcgattgccc gcgatgagcc
     9061 gcgtcagcat ctctgcgacc gcttcatcgg cgaagggctg ggtggcctgg tcgctgccga
     9121 ctgccccgta gaaccaggcg tgggcgacct cgtggatcac cgcgaattgg acgtctttgg
     9181 gcgcatagct atcggggatc cagaagccct gtggcgattc catcgcgaag cccgggctcg
     9241 tctcggcgat gaagagcttg ctgtacggat acgcgcccac caggggcacg tacttgtcga
     9301 aggcctgctt ggcgtacctg gcaaaggcgc tctgcgactg gttgatcgtg tagtactcga
     9361 actggacccc gccgtgcatc tccgtgccga cgtgatagtc cgggctggcg ctgaagttcc
     9421 agtcgcggac gttgtacgcg acgaagttct gggtgttgcc gttgaccgag gtgcgcgtcc
     9481 cgggcgcggc gtaaatgagg ccgccacggt cggccgtgat gctcacatcg acgcggctcg
     9541 caacctgggt cgtgaacgcg tcaccgaaga gcggcgtctt gaatgggtag cggttgctca
     9601 accacgggat ccagcggtac gcggtgacga tgccgtggcg cagggcgaac agccacttgc
     9661 ggttcgggtt ggtggagttg aacgtggccg agtagcggat cgtcacgtcg gcgctggcat
     9721 tgggcgcgag cgcgttgttg aggtgcacga tgaggttcat gtcatcgacc gtcgttgaag
     9781 ctgcgctgcc gttgacggtc acgccgttca tgtttatccg cccgatcttg gccggcacga
     9841 ggttgaacgt caggccggtc acgttcgagg acgacgtgtt cttgaccgtc gcggtgctgt
     9901 tcacgaccag ccgccgattg gaccactgca ggttggcgct gacgtcgtag gtcgcactca
     9961 agccgatgct gtcatttggt gcggcgtcaa cggccggcac gaaaaccgct gagagaaggg
    10021 caggcgctag aaccagggcg aggatatgga ttctcctttg cacggcgacc gtattatgcc
    10081 cgccgcctcc cggcggcgcg ttgcgctttc agatgggccg cgcgctcgcg ctggcggggt
    10141 tccggtcggc gtgagtcagc gcgcgcggcg gtagtgcatc gccaccgctc cgttgcgtag
    10201 tggcttcgcc gacatcaact cgagctcgcg cgtggcgggc agtccgccct ggtacagcgt
    10261 cgggccatgg ccggcgatcc tgggatggac gagcagcctg tactcgtcga tcaggtccag
    10321 ccggtcgagc tcggtcgcca gcttgccgct gccgagcagc actccggccg gcgtggcgtc
    10381 cttgagcttc tggacggcgt cgcgcaggtc gcccgcgatg tggtggctgt tgttccaggg
    10441 gaagtccgcg cgcgtcgccg agaccacgta cttgggcttg gcctccagct tgattgccca
    10501 ctcgcgcatg gccgatggcg cctcctcgtc gccgcgggcg acggccggcc agtagctctc
    10561 catcatctcg taggtgacgc gaccccagag catggccccg gcctcgtcca ggaggccggt
    10621 gtagaaggcg tgcgtctcgt cgtcggcgat cccctcgcgg tggtcgacgc aaccatccag
    10681 ggtgatgttg taactgaagg tgaggaggcc catggccggc gagtctaggg ctccagccgc
    10741 ctgacgagca ccggcttggt ggtcggggcg gtgggattcg aacccacgac ctcatgctcc
    10801 caaagcatgt gcgctgccgg actgcgctac accccgtgct cccatgatag ggcgcgtttc
    10861 cgctcggcct gagacgatga gcacttcgtg gaggccttcg ccctttcctt tggcgtgatc
    10921 ctgctggccg agcttggcga caagagccag ctcgtcacca tcgcattcac ggcacgctac
    10981 cggccggtgg tcgtcctcgc cggcatcgcc acagccgcag cgctcatcaa cggcttggct
    11041 gttgtcatcg gcgtggcctt cgccgcgatg ctgccgctcc atgtcctgca ggcgatcggc
    11101 ggcgtgctct tcctggcctt cgccgccatg acgttgtggc gcacggaagg cgacgacgaa
    11161 gagctg
//
LOCUS       DATBRX010000207         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1423873, whole genome shotgun sequence.
ACCESSION   DATBRX010000207 DATBRX010000000
VERSION     DATBRX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423873"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1298
                     /locus_tag="VIF08_08030"
     CDS             126..1298
                     /locus_tag="VIF08_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY8135970.1"
                     /translation="MSRREFVVAAAATVGTLALGCSLSEIASPTPKRTLGPTLPAVSP
                     TPSSTPTPAPTPPSLRRRAAAMLIAGFRGSALANEDPILRAIRDDGLGGVILFARNIQ
                     SPSQLRDLTTELRAAAGGRTLLIAIDQEGGAVARLNPDDGFPATPTAAKIGRRNDADY
                     ALRVGAAIAQTLADAGINLNLAPVVDLNVNPANPSIGALARSFSADPGVVVAMAEQFI
                     AGHRERGVLTALKHFPGLGSATGDTDREFVDITDTWSEAELDPFRRLIAAGAGDLVMV
                     GNALNGQLDQRNPASLSAPTVNLLRDDLGWRGAVITDDLMAGALSANFASDEVLRRAI
                     RAGNDLLLLANTARDAGDVVRPALEAIDALVARGEIDEALIDAANARVGRLLAPVG"
     gene            complement(1412..1969)
                     /locus_tag="VIF08_08035"
     CDS             complement(1412..1969)
                     /locus_tag="VIF08_08035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8135971.1"
                     /translation="MTKGAAARELLPFHGERVLLRDVRREDADLLDEWNAALEPDSFN
                     DFGPRAPTDRDALARGPLRNERNGTLIIERIEDSQPLGTIAYRRVSFGPPPNSDAWQL
                     GIDLAAEARGQGYGTEAQRLLADWLFATTPANRVEAATDIENVAEQRSLEKAGYVREG
                     VLRGSQFRAGAYHDLVYYSRLRDDP"
     gene            complement(2301..2747)
                     /locus_tag="VIF08_08040"
     CDS             complement(2301..2747)
                     /locus_tag="VIF08_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY8135972.1"
                     /translation="MSEGPRTRLAAYALCVEDGRVLLSRVAPGYPAAGLWTLPGGGVD
                     FGEDPADAARRELAEETGLEGRVDDLAFVNSISGEVSASGLGPWHGVRIVYLVTVTGG
                     TLRNEVDESSDLAEWATLPHLDGRPVVDLVDAALEWLGVERPSRAT"
     gene            2843..>3279
                     /locus_tag="VIF08_08045"
     CDS             2843..>3279
                     /locus_tag="VIF08_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8135973.1"
                     /translation="MSAKASAGKSVGQRAVVASKRDRSPDVGRGTPSPSSWSLGDGQQ
                     IPWEYAPAPESRDIVQLKSRYGLFIGGKEVAPRGGQWFTTVDPATEEPIAEIARATIK
                     DVDQAVSVARKAFRRDWSTLPGSERAKYLFRIARILQERSREFA"
BASE COUNT          529 a         1154 c         1101 g          495 t
ORIGIN      
        1 gacctgttcc gattcggcgc gagcagcctg ctcaacgacg tgctgatgca gatccgcaag
       61 gagaagaccg gggcatacca gggccccgac tacttcacga tcgactaggc gccgtgccgc
      121 gtcccttgag ccgacgcgag ttcgtggttg ctgccgccgc gacggtgggc acgctggcgc
      181 tgggctgttc gttgagcgag atcgccagcc cgaccccgaa gcgcacgctc gggccgacgc
      241 tccccgccgt atcgcccacg ccatcgtcga caccgacgcc cgcccccacg ccgccttcgc
      301 tgcggcggcg cgcggcggcg atgctcattg cgggatttcg cggcagcgcc ctggccaacg
      361 aggacccgat cctgagggcc atccgcgacg acggcctggg tggcgtgatc ctgttcgcgc
      421 gcaatatcca gtcgccctca cagctgcgcg acctcacgac cgaattgcgc gccgcagcag
      481 gcggtcgaac gctgctcatc gcgatcgacc aggagggcgg cgcggtggcg cgcctcaatc
      541 cggacgacgg ctttccggcc acgccgacag cggccaagat cggccggcgc aacgacgccg
      601 actacgccct gcgagtgggc gcggccatcg cccagacgct ggccgacgcg ggcatcaatc
      661 tcaacctggc gccggtcgtc gacctgaacg tcaacccggc caacccgtcg atcggagccc
      721 tggcccggag cttctcggcc gaccccggcg tggtcgtcgc catggccgaa caattcatcg
      781 ccggccaccg cgagcgcggc gtgctgacgg cgctcaagca cttccctggt ctgggcagcg
      841 caaccggtga caccgaccgc gagttcgtcg acatcaccga cacctggtcg gaggccgagc
      901 tcgacccgtt ccggcgcttg attgcggcgg gcgcgggcga cctggtcatg gtcggcaatg
      961 ccctcaacgg ccagctcgac cagcgcaatc cagcttcgct gtcagcgccc actgtcaacc
     1021 tgctgcgcga cgatctcggg tggcgaggcg cagtcatcac cgacgacctg atggcggggg
     1081 cgctgagtgc aaacttcgcc tcggacgagg tcttgcggcg ggccatccgg gccggcaacg
     1141 acctgctgct gctggccaac acggcccgtg atgcgggtga cgtcgtgcgg ccggcactcg
     1201 aggcgatcga cgcgctggtc gcgcgcggcg agatcgacga agcattgatc gacgccgcca
     1261 acgcccgcgt tggacggctt ctcgcgccgg tcggttgagt gcgccgtcag tcgacctggc
     1321 cgtgggatcc gagcacgcta cgcgcggttg tatctgcgat cgccctgccg atcgtgcttt
     1381 tcctgataac gcggatgctc gaccgcttcg tctaggggtc gtcgcgcagg cgcgagtagt
     1441 acacgaggtc gtggtacgcg ccggcccgga actgggagcc gcgcaggacg ccttcgcgca
     1501 cgtaaccggc cttctcgagc gagcgctgct cagccacgtt ttcgatgtcg gtcgctgcct
     1561 cgacgcggtt ggcgggcgtt gtcgcgaaga gccagtcggc gagcagccgc tgcgcctccg
     1621 tgccgtagcc ctggccgcgt gcctccgccg ccaggtcgat gcccagctgc cacgcgtcag
     1681 agttcggtgg cgggccaaag ctgaccctgc gataggcgat ggtgccgagc ggctggctgt
     1741 cctcgatgcg ctcgatgatg agcgtgccgt tgcgctcgtt gcgcagaggg ccgcgcgcca
     1801 gcgcgtcgcg gtcggttggc gcacgcgggc caaagtcgtt gaagctatcg ggctcaagcg
     1861 cggcgttcca ttcatcgagc aggtcagcgt cctcgcggcg cacatcgcgc agcaggacgc
     1921 gctcgccgtg aaacggcagg agctcgcgcg cggctgctcc tttggtcatc gtggtcggtc
     1981 gaaggctacc gccacgctgc cggcgatggg atcgatcgtg agcgccaagg cgacgtgggc
     2041 ttcgccgctg gcgatggcgc ttgcgcaccg ctgtggcggg tccgggtcaa ggactacggt
     2101 cgataccccc ttggcaaacc gacccaagcc gcgcagcagc gctagacccg ccaagggccg
     2161 cgcgcgcttc tcagttgtcc aaatcgccac gaaatcgacg ctgcgtgcct tcaaacctct
     2221 gtcggctagg gcgcgtctta ttgcccgtgc cgcctgcaga tcgtcggccg caccgaccac
     2281 catatgaggt gactgtttag tcacgtcgcg cgcgacggcc gctcgacgcc cagccactct
     2341 aacgcggcgt cgaccagatc gaccaccggc cggccatcga gatgcggcag tgtcgcccac
     2401 tcggcaaggt ccgacgactc gtcgacttcg ttacgcaagg tgccgccggt gacagtgacg
     2461 aggtacacga tgcgcacgcc gtgccatgga cccaggccgg atgcggacac ctcgccggaa
     2521 atcgagttca cgaaggcgag gtcatcgact ctgccctcga ggccggtttc ctctgccagc
     2581 tcgcgccgcg cggcatcggc gggatcttcg ccaaagtcga cgccgccacc cggcagcgtc
     2641 catagcccag cggcgggata acctggcgcg acgcgcgaca acaggacgcg cccgtcctcc
     2701 acgcacaatg cgtatgcggc caacctggtg cgcggcccct cgctcacgcc acaaggctag
     2761 caaacactca cgcgacaaca agtgaccaag aagtcaccta cggctgctag cgcacgaaac
     2821 gcgaccgatg taggctgaca tggtgagtgc aaaggcatcc gcgggtaagt cggtcggcca
     2881 gcgcgccgtc gtggccagca agcgcgaccg ctccccggac gttggtcgcg gcacaccctc
     2941 gccgtcgagc tggagcttgg gcgacggcca gcagatcccg tgggagtacg cgcccgcgcc
     3001 ggagtcgcgc gacatcgtcc agctcaagag ccgctacggc ctgttcatcg gcggtaagga
     3061 agtcgcaccg cggggtgggc aatggttcac gaccgtcgat ccggcgacag aggagccgat
     3121 agctgagatc gcccgcgcaa cgatcaagga cgtcgaccag gcggtgagcg tcgcgcggaa
     3181 ggcgttccgg cgcgactgga gcacgctgcc cggcagcgag cgcgccaagt acctcttccg
     3241 catcgcgcgc atcctccagg agcgcagccg cgagttcgc
//
LOCUS       DATBRX010000208         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2207015, whole genome shotgun sequence.
ACCESSION   DATBRX010000208 DATBRX010000000
VERSION     DATBRX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207015"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1095)
                     /locus_tag="VIF08_08050"
     CDS             complement(166..1095)
                     /locus_tag="VIF08_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135974.1"
                     /translation="MAEPIPPLRYLSAAELERCLPDIDARLDLAARALRALAHGSAEM
                     PPKIGVHPRPGALLHAMPAWLRDGDLVGLKWIAAFPDNRRRGLPAISGLMVLNDPDTG
                     APTWVMDAGRITAVRTAAVSGVALRLLAPPDVQRVLLVGAGGVQGGVHREVVGALLPG
                     AEIITAGTADEARREAGRAGVVITMAPIGAANHVLAPDWLADGTLTVSIDFATYASAA
                     LARDVGTFVVDDRAQFLAYRDAGHFDGYPEPTATLGELLDAQAAGQPIRPTGSHRLPA
                     LVNHLGVGLADVMFADAIRRRAEEMGAGQVLER"
     gene            1188..>2197
                     /locus_tag="VIF08_08055"
     CDS             1188..>2197
                     /locus_tag="VIF08_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY8135975.1"
                     /translation="MTTPTLELTAEERARSLANLKLERDAIVLYDSLAAIEKNPDRAR
                     VFERIAKNERRHAEIWASRLEGAGATVPPISAPRARVRLVIMLARLFGPERVADLVRG
                     LEGDEEAAYASQMTPETEAIAADEREHAEMWDQITGAKRLAANNGVRKTVTEEALHRG
                     GRSGTLRAAVFGANDGLVSNLSLVMGVAGAVADNHFIVLAGVAGLLAGAFSMAAGEYI
                     SMQSQREMFENQISVEREEMRVMPEVEREELAAIYRAKGIAPADADRIAGQLMQDPEK
                     ALDTMVREELGLDPDQLGSSWGAAIYSFVAFGIGAVIPLVPFLLSSGDVAFIAALVAS
                     FVA"
BASE COUNT          345 a          750 c          765 g          337 t
ORIGIN      
        1 tgcgctctgc acctgtcgcg cttcgaccta ttcagcggcg aggcgctcga tccaccggct
       61 gagctgccac tccagaccta cccggtaatc gtcgaagacg gcgagatcgt cctcgagctg
      121 cccgaggggc cgatccccgt caacgactag ccggcgcccg gctcctcacc gttctaggac
      181 ctggccggca cccatctcct ctgcgcggcg ccggatcgcg tcggcgaaca tgacatccgc
      241 caggccaacg ccgaggtggt tcaccagggc tggtagtcgg tggcttccgg tcggtcgaat
      301 cggttggccg gcggcttgcg catcgagcag ctcgcccagt gtcgcggtcg gctccgggta
      361 gccatcgaag tggcccgcgt cgcgataggc gaggaactgg gcgcgatcgt cgaccacgaa
      421 cgtgcccacg tcacgcgcca gggcggccga agcgtatgtc gcgaagtcaa tcgacaccgt
      481 gagcgtgccg tcggccagcc agtcgggcgc cagcacgtgg ttcgcggccc caatcggcgc
      541 catggtgatg acgacccctg cccgccccgc ctcgcgccgc gcctcgtcgg cggtcccggc
      601 cgtgatgatc tcggctccgg gaaggagcgc gccgacgacc tcgcgatgga caccgccttg
      661 gacgccgccg gctcccacta gcaataccct ctgaacatcc ggcggcgcga ggaggcgcaa
      721 agcgacgccg cttacggccg cagtgcggac cgccgtgatt cgcccggcat ccattaccca
      781 cgtgggcgcg ccggtgtcgg ggtcgttgag caccatcaga ccgctgatcg caggcaggcc
      841 gcgccgccgg ttgtccggga aggcggcaat ccacttgagc ccgaccaggt cgccgtcgcg
      901 caaccacgca ggcatcgcat ggagcagcgc cccgggccgc ggatggacgc cgatcttggg
      961 cggcatctct gcgctgccat gtgcaagggc acgcagcgcc ctggcggcga gatcgagccg
     1021 tgcgtcgatg tcgggcaggc agcgctcaag ctcggccgcc gacaggtagc gcaggggagg
     1081 tatcggttcg gccatggtgc gcgccagatt aggcttttcg gcaggccaac tcggagccgg
     1141 tcgtccagcg gcaccgggag ggtcgcgtcg ctaaccttga gggcgcgatg accactccca
     1201 cccttgagct gactgccgaa gagcgcgccc gcagcctggc caatctcaag ctggagcgtg
     1261 acgcgatcgt gctctacgac agcctcgccg cgatcgagaa gaacccggac cgcgcgcgcg
     1321 tcttcgagcg tattgccaag aacgagcggc gccacgccga gatctgggcg tcgcggctcg
     1381 agggggccgg cgcgaccgtg ccgcctatct ccgcaccaag agcgcgggtg cgtcttgtga
     1441 tcatgctcgc gcggctgttc ggccccgaac gcgtcgctga cctggtgcgc ggcctggagg
     1501 gcgacgagga ggccgcgtac gcgagccaga tgacgcctga gacagaagca atcgccgccg
     1561 atgagcgcga acacgccgag atgtgggacc agatcaccgg cgccaagcgt ctagcggcga
     1621 acaacggtgt gcgcaagacc gtgaccgagg aggcgctcca ccgcggcggc cgctctggca
     1681 ctttgcgcgc cgccgtgttc ggcgcaaatg atgggctggt cagcaacctg tcactcgtga
     1741 tgggcgtggc cggcgccgtc gcggacaatc atttcatcgt gctcgccggc gtcgccggtc
     1801 tgctggccgg cgccttctcc atggccgccg gcgagtacat ctccatgcag agccagcgcg
     1861 agatgttcga gaaccagatc tcggtcgaac gggaggagat gcgcgtcatg cccgaggtcg
     1921 agcgcgagga gctggcggcc atctaccgcg ccaagggcat tgcccccgcg gacgcggacc
     1981 gcattgccgg gcaactgatg caggacccgg agaaggcgct ggacaccatg gtgcgcgagg
     2041 agctcgggct ggatcccgac cagctcggct cgtcgtgggg tgcggcgatc tattcgttcg
     2101 tggcctttgg aattggcgcc gtcataccgc tcgtgccgtt cctgctctcg agtggtgacg
     2161 tggcgttcat cgccgcgctg gtggcatcgt tcgtggc
//
LOCUS       DATBRX010000209         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_421161, whole genome shotgun sequence.
ACCESSION   DATBRX010000209 DATBRX010000000
VERSION     DATBRX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421161"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VIF08_08060"
     CDS             <1..459
                     /locus_tag="VIF08_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8135976.1"
                     /translation="ITRKNMEMTLDVMANSLVYWAQDVWRAGLFGDGSKIYAMTSEGS
                     SRVIPSYGAVSAAKSALESNCRQLAMEFAKLGSGVTVNAIRAGVTKTPALMQLPEAQD
                     LIDHALRRNPMGRLASVDDVASAIVAFSGEGTHWMTGNVLGIDGGELVTG"
     gene            complement(456..>1908)
                     /locus_tag="VIF08_08065"
     CDS             complement(456..>1908)
                     /locus_tag="VIF08_08065"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY8135977.1"
                     /translation="GLSMDYEVFLLSRMKEVYDKTGDNRAAVARGMERSGRIVTSAAL
                     VVVVVAGSFAFADIVLIKALGLGVAIAVALDATLVRGLLVPSAMRLLGNVNWWMPARL
                     KRLLPPLAALLLIGPLLLACDANGRLLSNEPAQHPTVPPAAPTDPPPTDPQPVVFPRD
                     EAAHDRLTEWWYYTGHLWPSHGGDPDGPHYGFEFVIFRAERGGFPVSWASHLALTDEN
                     AGAFGYAQRSEIGPQVDVSEDTGFDLRIHGSSDNGTIDSAAAPWEMAGLDGSDGLDAA
                     TSDFGINLLLNDTRTAPVLHDTDGWIDFGDAGSSYYYSRPRMTASGTLTVRDQAMEVT
                     GEAWFDHQWGDFIAVGGGGWDWFAVNLDDGTDLTLSLVRAADGTYPLVYGTLVRATGE
                     TVHLPRDEFTITSDTTWTSPDTGAVYPSGWTIDIPGEQLAINLTPTVLDQELDTRQTT
                     GVIYWEGSQIVTATRAGQTVNGEGYVELTGYAP"
BASE COUNT          319 a          634 c          652 g          303 t
ORIGIN      
        1 atcacgcgca agaacatgga gatgacgctc gacgtcatgg ccaattcgct cgtctactgg
       61 gcgcaggacg tgtggcgcgc cgggctgttc ggtgatggct cgaagatcta cgccatgacg
      121 agcgagggct cgtcgcgcgt catcccgtca tacggcgcgg tcagcgcggc caagagcgcg
      181 ctcgaatcga actgccgcca gctcgcaatg gagttcgcca agctgggcag cggcgtaaca
      241 gtgaacgcca tccgggccgg cgtcaccaag accccggcgc tgatgcagct gcccgaggcg
      301 caggacctga tcgatcacgc actgcggcgc aacccgatgg gtcgcctcgc gtcagtcgac
      361 gatgtcgcgt ccgcgatcgt cgcgttcagc ggcgagggca cgcactggat gaccggcaac
      421 gtcctcggga tcgacggcgg cgagttagtc accggctagg gtgcgtaccc ggtcagctcc
      481 acatagccct cgccgttcac cgtctggccg gcgcgcgtcg cggtgacgat ctgcgacccc
      541 tcccagtaga tgacgccggt cgtctgccgc gtatccagct cctggtccag gacggtgggc
      601 gtgaggttga tcgcgagctg ctctccggga atgtcgatcg tccatcccga cgggtagacg
      661 gcgcccgtat ccggactcgt ccaggtcgtg tccgacgtga tcgtgaactc gtcgcgcggc
      721 aggtgaaccg tctcgcccgt ggcgcgcacg agcgtgccgt agaccagtgg gtaggtcccg
      781 tccgcggccc gcaccagcga cagcgtgagg tcggtgccgt cgtcgaggtt caccgcgaac
      841 caatcccagc cgccccctcc gacggcgatg aaatcgcccc actggtgatc gaaccaggcc
      901 tcgcctgtga cttccattgc ctggtcgcgg acggtcaggg tgccgctcgc ggtcatgcgc
      961 ggccgcgagt agtagtacga cgagccggcg tcgccgaagt cgatccagcc gtcggtgtca
     1021 tgcagcacgg gtgccgtgcg ggtgtcgttc agcaacaggt tgatgccgaa gtcgctcgtc
     1081 gccgcgtcga gcccatcgct gccatcgaga ccggccatct cccacggtgc ggccgctgaa
     1141 tcgatcgtgc cgttgtcgct gctgccgtgg atgcgcaggt cgaaccccgt gtcttcgctg
     1201 acatccacct gcggcccgat ctcggagcgc tgcgcatagc cgaatgcacc ggcgttttca
     1261 tcggtgagcg cgaggtgcga cgcccacgac acggggaagc cgccgcgctc agcccggaag
     1321 atcacgaact cgaagccgta atgcggccca tccggatcac caccatggct cggccagagg
     1381 tggccggtgt agtaccacca ctcggtcagt cggtcgtggg cggcctcgtc gcgcgggaag
     1441 accaccggct gcggatcggt tggcgggggg tcagtcggag cggcgggcgg gacagtcgga
     1501 tgttgggcgg gctcgttcga gagcaggcgg ccgttggcat cgcaggccag caggagcggg
     1561 ccgatcaaca gcaacgctgc caatggcggc aaaagtcgct tcagccgcgc gggcatccac
     1621 cagttgacgt tgcccagcaa cctcatcgcc gaggggacga gcagtccgcg aacgagagtt
     1681 gcgtcgaggg ccacggcaat tgcgacgccc aggccgagcg ccttgatgag cacgatgtcg
     1741 gcgaaagcga aggagcccgc gacgacgacg acgaccagtg ccgccgacgt gacgatccgg
     1801 ccgctgcgct ccatgccgcg cgcgaccgcc gcgcggttgt cgccggtctt gtcgtagacc
     1861 tccttcatgc gcgacagcag gaatacctcg taatccatcg ataggccg
//
LOCUS       DATBRX010000210         7884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3213936, whole genome shotgun sequence.
ACCESSION   DATBRX010000210 DATBRX010000000
VERSION     DATBRX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3213936"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..447)
                     /locus_tag="VIF08_08070"
     CDS             complement(169..447)
                     /locus_tag="VIF08_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135978.1"
                     /translation="MLYQLAIHRPKPEHEQDLIDSMHRFGSAARTEGAIEAHTLKDER
                     SGALVGLAIWRSDNDLAAARPALAAATQDDDFATWESGPIEVFMLRDV"
     gene            592..1290
                     /gene="folE"
                     /locus_tag="VIF08_08075"
     CDS             592..1290
                     /gene="folE"
                     /locus_tag="VIF08_08075"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY8135979.1"
                     /translation="MDRKLPDRVRLLVDEMEREVGEDGFDDGFVDIDGQHDGDGSTDG
                     SQAVESAVRQILVEMGEDPDRQGLADTPSRVRRMYRELTAGYWVDPERLVNKAIFDVD
                     YSEMVVVKDIAFYSLCEHHLLPFFGQAAVAYIPNGRVIGLSKIPRIVEMFSRRLQVQE
                     RMTRQIADFLMERLQPKGVGVVMEASHLCAVMRGVRKPGTIMTTSHVLGLFRTADRTR
                     AEFFSHLERRPPAL"
     gene            1301..2155
                     /locus_tag="VIF08_08080"
     CDS             1301..2155
                     /locus_tag="VIF08_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135980.1"
                     /translation="MSDIERVPSMRKDAKQGVPKRKPRDMAKPMAESGKTKDAPAVAP
                     RTVAPEPLLNDPMLDEPMDNQALTEMPTLPTEPAHEPDAEPPAIAPPEREPEAELAAV
                     SEPEPMPQQDPPAVEVAPPAVDRNAPPLTMRDMFARVEPAFHQFRDAAYRYPAEHMND
                     RIGEGGWTRKQMLSHIAAWHDLTTDRLLKMALTGQPVALDRDQEAVNAVAARVAVGKT
                     AGEVLKDVEASYARLRRQMLRMTDADLRLGDGWAAGRIASNTYEHYGEHIADLAPPVA
                     LPGSGPQR"
     gene            2169..2585
                     /locus_tag="VIF08_08085"
     CDS             2169..2585
                     /locus_tag="VIF08_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEY8135981.1"
                     /translation="MTDATARPIKVLIAKPGLDGHDRGAKVLARGLRDEGFEVIYTGL
                     RQTPEMVVSAALQEDVDVVGLSILSGAHMTLLPKITAGLHERGMDDVLVTAGGIIPDD
                     DIPQLEQAGVSRVFGPGTTIREVAEYIRANVKSRGE"
     gene            2599..3588
                     /gene="meaB"
                     /locus_tag="VIF08_08090"
     CDS             2599..3588
                     /gene="meaB"
                     /locus_tag="VIF08_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY8135982.1"
                     /translation="MTDTSLAEKARSGDRRALARLLTQVENRTAAGEAALKTLYPLAG
                     RAHLVGVTGAPGSGKSTLVAALTGVARLAGRPVAVIAIDPSSPITGGAILGDRVRMQI
                     HATDDDVFIRSMASRGHSGGLAASTVAAAAVIDAAGFGLIFIETVGTGQSEVEVAAIA
                     DTTVVVQSPEMGDEVQAIKAGLLEVADIVVVNKSDRPGADRAAAQLRAMLTVGASADK
                     AALAEGRPRPKRPEVMLCSALTGAGVADLLAALDRRAPAADSRLADGAALKRAEAQIS
                     GILALRINAQLRDPGRATARDETLRAVAAHEIDPFTAADRLLALLGERAAARD"
     gene            3601..4467
                     /locus_tag="VIF08_08095"
     CDS             3601..4467
                     /locus_tag="VIF08_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8135983.1"
                     /translation="MTQRNVLVCGAGLMGHGIAQVMAAAGHSVALYEPEADRAEAGRK
                     RIEGNLDRAVAKGKLTQDERDQVLGRVSVAPVLAEAAKSAELVVEAVFEDESVKGKLF
                     ADIARSAPESAILASNTSSIAITGLAAALPASRRANFVGMHFFSPVPVMPLIELIRGA
                     DTSDATESYVREMAAELNKKVIVSQDRPGFIVNRVLMPFLAEAMRAYEEGLATAEDID
                     TGAKVGLNHPMGPLELADFIGLDVCLHIMEVLHEGFDEPHFAPPDVLRDLVEAGHLGQ
                     KTGRGFYTYPRA"
     gene            4464..5639
                     /locus_tag="VIF08_08100"
     CDS             4464..5639
                     /locus_tag="VIF08_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8135984.1"
                     /translation="MNGHNGALTEEERLLQKTVRDFAAKELAPGAAERDETEQYDRSL
                     FARMGELGLTAVPFPTEIGGAGFSYRAWTLVMEEVAYADMAMAVSLSVHILAQYPVIT
                     WGNDSQKQRWLPDMLAGKKLGAFGLTEPAAGSDAGALRLKADRVGPADSPTAYRLTGT
                     KIWITNAGEAERYLVFATVDPGLGTKGITAFLVEAGTPGFTFGKREKKLGIRSDTASE
                     LIFEGCEVPVANRLGEEGQGYRIALSALGEGRISIGAACVGLAQAALDAAAKYLTERK
                     AFGAPLSDQQGLRFMLAEMAQKVAAARALVRAAAETKDRGENIAEASSLAKWTASDAA
                     MSVTTDAVQLFGGSGYSREFPVERMMRDAKGAQIYEGTNQIHRLIVADQVLRRVKES"
     gene            5918..7171
                     /locus_tag="VIF08_08105"
     CDS             5918..7171
                     /locus_tag="VIF08_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135985.1"
                     /translation="MISDFPPAGANVAAITTFAGGFVAVGTAQEPDYCDGSHPVVGQV
                     WTSADGSAWSEADVDLTGLEPRHVVVAGGVLYVFLCTSGQHLVARSADGLEWRVDQVN
                     VPNWYASGYAALGDTLLASAQAYDDEGRVLASVWSSSDGVNWAPQPGSPETESGFGLS
                     GLAASGDFVVAYSADARPMFVSTDGGTTWHQADHAPLYNADVVAVAMSGGHFAAVGEA
                     CCTAPREYVGFGIWSDDALHWRESQPFGSREIPEGIVALPGGFVTIGRQSWLSSDGGS
                     WVIGPEIPGYEAGRSYQRAIGAASSEVVVITNGATAWIAGLADLDPGLYSDIARVADL
                     PSVGTSYGLSIYTHCGWPPLHFDLRAWVPDPPINAHNPPPGLKQNDHGTLTYVSEDEL
                     RYDSERGRVIKLVPSDEPTPIGGCA"
     gene            7225..>7884
                     /locus_tag="VIF08_08110"
     CDS             7225..>7884
                     /locus_tag="VIF08_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY8135986.1"
                     /translation="MTQDRQSPAGPNGSVFIVSAARTPIGKFAGTLADTPATTLGGIA
                     IRAAVERAGIANDAVDEVLMGQVLQAGVGQAPARQALLKGGLADSTSATTINRVCGSG
                     LKAIMLAAAEIRAGDAEIVVAGGMENMNQGPYLIPKARFGYRLGNAEMIDATVHDGLW
                     CSVEGCHMGTHAERVAIKDSVSRADQDQFALESHQRAIAAINEGRFRDEIVAVPVRKG
                     KE"
BASE COUNT         1332 a         2642 c         2750 g         1160 t
ORIGIN      
        1 ctcgcctgct ggatggcgcc cgggttgggc ggggcgtgat agaagcgcgt gtcagctggc
       61 agcgcaggag tggtcgccac gaaccgtgca gtcgggacga acaggcagag caacgcgccc
      121 accacagcca ggaatcgcat cgcctcgatc ttcgcagacc tgcgttcgct agacgtcgcg
      181 gagcatgaag acctcgatcg gcccactctc ccacgtggcg aagtcgtcgt cctgggtggc
      241 ggccgccagc gcgggccgcg cggccgcgag gtcgttgtcc gaccgccaga tcgcgagacc
      301 gacgagcgcg ccggaccgct cgtccttcaa cgtgtgcgcc tcgatcgcgc cctcggtgcg
      361 cgctgcactg ccgaaccggt gcatcgagtc gatcaggtcc tgctcgtgct ccggcttggg
      421 acggtggatc gcgagctggt agagcatcgg ttctccttcc ttttgcagcg acgaggcgac
      481 ggctcatgca gggtagagcg tctatcccgc gccacatccc cgtgagcgag tggaggcgcg
      541 ggttgagcga ctgaatgggc gggatccaga aggggtgtca aactgggcgc gatggatcgc
      601 aaactgcccg atcgcgtgcg cctgctggtc gacgagatgg agcgcgaggt tggcgaagac
      661 ggcttcgacg acggattcgt ggacatcgac ggtcagcatg acggcgacgg ctcgacggac
      721 ggctcgcagg cggtcgaatc ggcggtgcgc cagatcctgg tcgagatggg cgaggatccc
      781 gaccggcagg gcctggccga tacgccgtcg cgcgtgcggc gcatgtaccg cgagctgaca
      841 gcgggctact gggtcgatcc ggagcggctc gtaaacaagg ccatcttcga tgtcgactac
      901 agcgagatgg tcgtcgtcaa ggacatcgcc ttctacagcc tgtgcgagca ccatctgctg
      961 ccgttcttcg gtcaggccgc ggtggcgtac atccccaacg gccgggtgat cggcctgtcc
     1021 aagatcccgc gcatcgtgga gatgttctcg cgccggctcc aggtgcagga gcgcatgacg
     1081 cgccagatcg ccgacttcct gatggagcgg ctgcagccca agggcgtggg cgtcgtgatg
     1141 gaggcatcgc acctgtgcgc ggtgatgcgc ggcgtgcgca agccgggcac gatcatgacc
     1201 acgtcgcacg tcctcgggct tttcaggacg gccgatcgga cgcgggccga gttcttcagc
     1261 cacctcgagc gcaggccacc cgcgctgtga gcgagctgac gtgagcgaca tcgaacgcgt
     1321 cccatccatg cgcaaggacg ccaagcaggg cgtgcccaag cgcaagccgc gcgacatggc
     1381 caagccgatg gcggaaagcg gcaagaccaa ggacgcaccg gccgttgcac cgcggaccgt
     1441 tgcaccggag ccgttgctca acgacccgat gctcgacgag ccgatggaca atcaggcgct
     1501 gaccgaaatg ccgaccctgc cgacggagcc agcgcatgag ccggacgcgg agccgcctgc
     1561 catcgcgcca cccgagcgcg aaccggaagc ggagctggct gctgtctcag agcccgagcc
     1621 gatgcctcag caagacccgc ctgccgtcga ggtggcgccg ccagccgtcg accggaacgc
     1681 tccgccgctg acgatgcgcg acatgttcgc gcgcgtcgag ccggcgttcc accagttccg
     1741 cgacgcggcc tatcgctacc cggcggagca catgaacgac cgcatcggcg aaggcggttg
     1801 gacgcgcaag cagatgctct cgcacatcgc cgcgtggcac gacctgacga cggatcgcct
     1861 gctcaagatg gcattgaccg gccagccggt agcgctcgat cgcgaccagg aggcggtcaa
     1921 tgcggtggcc gcgcgggtgg ccgtgggcaa gacggcaggc gaggtgctga aggatgtcga
     1981 ggcgagctat gcccggttgc gtcgccagat gctgcgcatg accgacgccg acctgcggct
     2041 gggcgacggg tgggcggcag gacggatcgc gagcaacacg tacgagcact acggcgagca
     2101 catcgccgac ctcgccccgc ccgttgcgct gccgggaagc ggcccgcagc gctgactatc
     2161 ctctgaccgt gactgacgca accgcacgcc cgatcaaggt gctcatcgcc aagcccggcc
     2221 tcgacggcca cgaccgcggc gccaaggtgc tggcgcgcgg gttgcgcgac gagggcttcg
     2281 aggtgatcta caccggcctg cgccagaccc cggagatggt cgtcagcgcg gcgctccagg
     2341 aagacgtgga tgtcgtgggg ctgtccatcc tgtcgggcgc gcacatgacg ctgctgccca
     2401 agatcaccgc cggcctgcat gaacgaggca tggacgacgt gctggtgacg gccggcggga
     2461 tcatcccgga cgacgacatc ccgcagctcg agcaggccgg cgtgtcgcgc gtctttgggc
     2521 cgggcacgac catccgcgaa gtggccgagt acatccgcgc gaacgtcaag tcccgcggag
     2581 agtgaagccg cgcagggagt gacggacacc tcgctcgccg agaaagcgcg gtcgggagat
     2641 cgccgcgcgc tggcccgatt gctgacgcag gtcgagaacc gcaccgcggc gggggaggcc
     2701 gcgctcaaga cgctctaccc gttggcaggc cgcgcccacc tggtgggcgt gacgggtgcc
     2761 ccggggagcg gcaagtccac cttggtcgca gcgctcacgg gcgtggcgcg cctggccggc
     2821 cgcccggtcg cggtcatcgc catcgacccg tcctcgccca tcaccggcgg cgccatcctg
     2881 ggcgaccgcg tgaggatgca gatccacgcc acggacgacg acgtgttcat ccgctcgatg
     2941 gcgtcgcgcg gccactcggg tgggctggcc gcgagcacgg tcgcggcggc ggcggtcatc
     3001 gatgcggccg gcttcggttt gatattcatc gaaacggtcg gcaccgggca aagcgaggtc
     3061 gaggtcgccg ccatcgccga cacgacagtc gtcgtccagt cgcccgagat gggcgatgag
     3121 gtccaggcga tcaaggccgg tctgctggaa gtggcggaca tcgtggtcgt caacaagtcc
     3181 gaccgacccg gcgcggaccg cgccgccgcg cagctgcggg cgatgctgac ggtgggcgcg
     3241 tccgcggaca aggccgcact cgccgaaggc cggccgcgtc ccaagcggcc agaggtgatg
     3301 ctctgctcgg cgctgaccgg cgccggcgtg gccgacctgc tcgccgctct cgatcgccgc
     3361 gcgcccgccg ccgactcgcg tctggcggat ggcgcggccc tcaaacgagc ggaggcccag
     3421 atcagcggca tcctcgcgct gcgcatcaac gcgcagctgc gcgacccggg gcgcgcgacg
     3481 gcccgcgacg agacgctgcg tgccgtcgcg gcgcacgaga tcgatccctt caccgccgcc
     3541 gaccggctcc ttgccctgct gggcgagcgc gccgcggctc gggactaggc ttaacgacgt
     3601 atgactcaac gaaacgtcct tgtctgcggc gccgggttga tgggccacgg catcgcccag
     3661 gtcatggccg cggcaggtca cagcgtggcc ctgtatgagc cggaagcaga ccgcgcggag
     3721 gctggccgaa aacgcatcga aggcaacctc gatcgcgccg ttgccaaggg aaagctcacc
     3781 caggacgagc gcgatcaggt cctgggccgg gtgtcagtcg caccagttct ggctgaagcg
     3841 gcgaagtcgg ccgagctggt ggtcgaagcc gtcttcgagg acgaatcggt caagggcaaa
     3901 ctcttcgccg acatcgccag gagtgcgccc gagtcggcca ttctcgccag caacacgagc
     3961 tccatcgcga tcaccgggct cgccgccgcg ctgcccgcga gccgccgcgc caacttcgtc
     4021 ggcatgcatt tcttctcgcc cgtgccggtg atgccgctga tcgagctgat ccgcggcgcg
     4081 gacacgtcgg acgccacgga gtcctacgtg cgcgagatgg cggcggagtt gaacaagaag
     4141 gtcatcgtca gccaggaccg gcccggcttc atcgtcaacc gggtgctcat gccatttctc
     4201 gcggaggcga tgcgtgccta cgaggagggc ctggccaccg cggaggacat cgacaccggc
     4261 gccaaggtcg gcctgaacca tcccatgggc ccgctggagc tggccgattt catcggcctc
     4321 gatgtctgcc tgcacatcat ggaagtgctc cacgagggct tcgacgagcc gcacttcgcc
     4381 ccgccagacg tgctgcgcga cctggtcgag gcggggcatc tgggccagaa gacggggcgc
     4441 ggcttctaca cctatccccg cgcgtgaacg gccacaacgg cgccctgaca gaggaggagc
     4501 gcctcctcca gaagacggtg cgcgactttg ccgcgaagga actggcgccg ggcgccgcgg
     4561 agcgcgacga gacagagcag tacgaccgct cgctctttgc gcgcatgggc gagctgggtc
     4621 tgaccgctgt gcctttcccg acggaaatcg gcggcgcggg cttcagctac cgcgcgtgga
     4681 cgctcgtcat ggaagaggtc gcgtacgccg acatggccat ggcggtgtcg ctgagcgtcc
     4741 acatcctggc gcaatacccg gtcatcacct ggggcaacga ctcgcagaaa cagcgctggc
     4801 tgccggacat gcttgccggc aagaagctcg gcgcgtttgg gctgactgag ccagcggcgg
     4861 ggtcggacgc tggcgccctc cgattgaagg ccgaccgagt cggaccggcc gattcaccca
     4921 ccgcctaccg gctcaccggc accaagatct ggataaccaa cgccggtgag gcggagcgct
     4981 acctggtctt cgcgaccgtt gacccagggc tgggcacgaa gggcatcacc gcgttcctgg
     5041 tcgaggccgg cacgcccggc ttcacctttg gcaagcgcga gaagaagctg ggcatccgct
     5101 ccgacacggc gagcgagctc atcttcgagg gctgcgaggt gcccgtcgcg aaccgcctcg
     5161 gcgaggaggg gcagggctac aggatcgcgc tctcggccct gggggaggga cgaatatcca
     5221 ttggcgcggc gtgcgtcggt ctcgcgcagg cggcgctgga cgcggccgcg aaatacctga
     5281 ctgagcgcaa ggccttcggc gcgccgctct ccgaccaaca gggcctgcgc ttcatgctcg
     5341 cggagatggc ccagaaggtg gccgcggccc gagcgctcgt gcgtgcggca gcggaaacaa
     5401 aggaccgcgg cgagaacatc gccgaggcct cgtcactcgc caagtggacc gcctcggacg
     5461 cggcgatgag cgtcacaacc gatgccgtgc agctctttgg ggggagcggc tactcgcgtg
     5521 aattccccgt cgagcggatg atgcgcgacg ccaagggcgc gcagatctac gagggcacca
     5581 accagatcca tcggctgatc gtcgccgacc aagtgctccg ccgggtcaag gagagctgac
     5641 ggcaccacat cggtgcgcga cggttcgtcc cagcgtcatg cctagacccc agtctgtgac
     5701 gctgacgctc gcgttcctga tcgctgtctc actgagcggt tgcgcgtacg tcggcgaggc
     5761 aacaccctcc ccaggcatct ctccgacacc cgcatctccg acgcccgcat cacccacatc
     5821 cacgccagcc caggtgccga ctccgactca ggaaccaggg cctacgccca cggcggagcc
     5881 cacacccagt catcagcctg tcactggtgg gtggcatgtg atcagcgact tccctccggc
     5941 gggggcgaac gtggccgcca tcacgacgtt cgccggtggc ttcgtggccg tcggcaccgc
     6001 ccaagagccg gactactgcg atggcagcca cccggtagta ggtcaggtct ggacgtccgc
     6061 agatggatcg gcctggtcgg aagcagatgt cgacctgacg ggcctggagc cgcgccacgt
     6121 cgttgtcgcg ggtggagtgc tgtatgtgtt tctctgcacg tcgggccagc atttggttgc
     6181 gcgttctgcc gacggcctcg agtggcgcgt cgaccaggtg aacgtgccta actggtatgc
     6241 aagcggctac gcagcgttag gcgacaccct gctggcgtcg gcgcaagcgt acgacgacga
     6301 gggccgggtc ctggcgtccg tatggagctc gagcgacggc gtcaactggg cgccgcagcc
     6361 aggcagcccc gagaccgaat ccggtttcgg tctcagcggt ctcgctgcat cgggcgactt
     6421 cgtcgtcgcg tatagcgcgg acgcgcgccc catgttcgtc tccacggatg gtggcacgac
     6481 gtggcaccag gctgaccacg cgccgctgta caacgccgat gtagttgccg tcgcaatgtc
     6541 cggtggccac ttcgcggccg tcggcgaagc ctgctgcacg gcacccaggg agtacgtcgg
     6601 ctttggaatc tggtcggacg atgctcttca ctggcgggag tcccagccct tcggcagccg
     6661 cgagattccc gaggggatcg tggcgttgcc aggcgggttc gtgaccatcg gccggcagag
     6721 ctggctctcg agcgacggtg gcagctgggt aatcggcccg gagattccag gctacgaagc
     6781 cggccggagc taccagcgtg ccatcggggc cgcttcctcc gaggtagtgg tgatcacaaa
     6841 tggcgcgacc gcgtggatcg ccgggctcgc cgatctcgac ccaggcctgt attcggatat
     6901 agcgcgcgtc gcggatctgc cgtcggttgg aacgagctat ggcttgagca tctacacgca
     6961 ctgtggctgg cccccgctgc acttcgactt gcgagcctgg gtgcccgatc caccgatcaa
     7021 cgcccacaac ccaccgccgg gcctgaagca aaacgatcac ggaacgctca cctacgtcag
     7081 tgaggatgag ctccgctacg acagcgaacg cggccgagtc atcaaactgg taccgagcga
     7141 cgagccgaca ccaattgggg gctgcgccta gcgtgcaagt cattgccgac tgcggacggg
     7201 gtggtcggtc gtaaactggc acgcgtgacc caggatcgac agagtccggc cgggccgaac
     7261 ggcagcgtgt tcatcgtgtc cgccgcgcgg acgcctatcg ggaagttcgc cggcaccttg
     7321 gccgacacgc ccgcgaccac cctcggcggc atcgccatac gcgccgcggt cgagcgcgcc
     7381 ggcatcgcca acgacgccgt cgacgaggtt ctcatgggcc aggtgctgca ggccggggtc
     7441 ggccaggcgc cggcgcgcca ggcgctgctc aaaggcggtc tcgccgattc gaccagcgcc
     7501 acgaccatca atcgcgtctg cggcagcggc ctgaaggcga tcatgctcgc cgcggcggag
     7561 atccgcgcgg gcgacgccga gatagtggtc gcgggtggca tggagaacat gaaccagggg
     7621 ccgtacctga tccccaaggc gcgcttcggc taccggctcg gcaacgcgga gatgattgac
     7681 gcgacggtcc acgacgggct gtggtgcagc gtcgagggct gccacatggg cacccacgcc
     7741 gagcgggtgg cgatcaagga ttcggtcagt cgcgccgacc aggatcaatt cgcgctggaa
     7801 tcgcaccagc gcgcaatcgc ggcgatcaac gaaggtcgct tccgcgacga gatcgtggcg
     7861 gtgcccgtac ggaagggcaa ggaa
//
LOCUS       DATBRX010000211         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_309260, whole genome shotgun sequence.
ACCESSION   DATBRX010000211 DATBRX010000000
VERSION     DATBRX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309260"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VIF08_08115"
     CDS             <1..275
                     /locus_tag="VIF08_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135987.1"
                     /translation="SGELDLSTDELTKQLEASFLKLRLRDASVVALPDDAAAPPDTIL
                     GAMTRDLRARIEDHETRGDMERAAELREALRLGTALLDDPTRVSLA"
     gene            278..>1582
                     /locus_tag="VIF08_08120"
     CDS             278..>1582
                     /locus_tag="VIF08_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8135988.1"
                     /translation="MIITRLRLTNFRRHRALDVQLGPGLNVVRGANEAGKSTIQRAIE
                     MALFRRPTFSGNELDDTRPWQDRAADPEIELQFTDEGRAGTIHKAFAGGKGTVTMSLG
                     GETFNDPATVDQHVVALTGIPSEKFFRASASVHHQELTGLAQDENTLRDRLQQSMSGA
                     DGGTHAARRKLEEAIRRYRTEGAKNPGYLKVMRTEAERLRDQVRRGEMALAQLEADRR
                     ALADARAARTQVDARLAEQREGAARAERAAALNRRLTDAAKQYAQYKRAAELREEIGR
                     LEASHPSAIALPVLRSTVEHLRRLEFGLSEMRAEMAAEPDLSGYDVAISAPRWRPLVV
                     LGLLATLAALGALAAGFVVLGLSAISFPGAVGFAVLALACFLLAVRRRTRQTNVRLQN
                     ELREGDIARRLAGRTDLAERVRQAEQERVEGLTPLGMADLAAA"
BASE COUNT          242 a          551 c          574 g          215 t
ORIGIN      
        1 gctctggcga gctggacctc agcacggacg agctcacaaa gcagctggag gcctcgttcc
       61 tgaagctgcg cctgcgcgac gcgtcggtcg tcgccctgcc tgatgacgcg gcggcgccac
      121 cggacacgat cctgggggcc atgacgcgcg acctccgggc acgcatcgaa gaccacgaga
      181 cgcgcggcga catggaacgc gcggccgagc tgcgcgaagc actccgcctg ggaacggcgc
      241 tgctcgatga ccccacccgg gtgtcgctgg cctgacgatg atcatcaccc gtctgcgcct
      301 cacgaacttc cgccgccacc gcgccctgga tgtgcagctc ggccccggcc tgaacgtggt
      361 gcgcggcgcc aacgaggccg gcaagtcgac catccagcga gcgatcgaga tggccctctt
      421 ccgccgccca acgttcagcg gcaacgagct cgacgacacc cggccgtggc aggaccgcgc
      481 ggctgatccg gagatcgagc tccagttcac cgacgagggg cgcgcaggca cgatccacaa
      541 ggccttcgcc ggcggcaagg gcacggtcac catgtcgctg ggcggcgaga ccttcaacga
      601 tccggcgacg gtcgaccagc acgtcgtggc cctgactggc atcccgagcg agaagttctt
      661 ccgcgccagc gcgagcgttc atcaccagga gctgaccggc ctcgcccagg acgagaacac
      721 gctgcgtgac cggctgcagc agtcgatgag cggcgccgat ggcggcacgc acgccgcgcg
      781 gcgcaagctg gaggaggcga tccgccgcta ccgcaccgaa ggcgccaaga acccgggtta
      841 cctgaaggtg atgcgcactg aggccgagcg gctgcgcgac caggttcgcc gcggcgagat
      901 ggcgctggcc cagctcgagg ccgatcggcg cgcgctggcc gatgcgcgcg cggcgcgaac
      961 gcaggtcgac gccaggctcg ccgagcagcg tgagggcgcg gccagggccg aacgggcggc
     1021 cgcgctgaat cgcaggctga ccgacgccgc gaagcagtac gcgcagtaca agcgcgccgc
     1081 cgagctgcgt gaggagattg gcaggctcga ggcgagccac ccgtcagcga tcgcgctgcc
     1141 ggtgctgcgc tcgacggtcg agcacctgcg caggctggaa ttcggcctgt ccgagatgcg
     1201 cgccgagatg gcggctgagc cggacctgtc gggttacgac gttgccattt ccgcgccgcg
     1261 ctggcgcccg ttggtggtcc tcggcctctt ggccacgctg gctgctctgg gggctttggc
     1321 tgcaggcttc gtggtgctcg ggttgtccgc gatctccttt ccgggcgctg tggggttcgc
     1381 ggtcctggca ctggcgtgtt tcctgcttgc cgtccgccgg cgcacgcgcc agaccaacgt
     1441 ccggctgcag aacgagttgc gcgagggaga catcgcgcgc cgcctggcgg gtcggacgga
     1501 tctcgcggag cgcgtccgcc aggctgagca ggagcgcgtc gagggcctga caccgctggg
     1561 catggcggac ctcgcggctg cg
//
LOCUS       DATBRX010000212         3912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_757023, whole genome shotgun sequence.
ACCESSION   DATBRX010000212 DATBRX010000000
VERSION     DATBRX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757023"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VIF08_08125"
     CDS             <1..457
                     /locus_tag="VIF08_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135989.1"
                     /translation="GGPPPPPRTTPVTARFDTSAGLRPRNSRTGAPPATMRGMYPRAR
                     RDSGGERDLRASTPTGPVHRRTGRRAEPMPTLQESRETTLNFGRFHGYTLGEVELLEP
                     SYLDWVARTITRDRDLVVKARLIQQDLDERGVARPSRAATPGFGPTFDG"
     gene            737..2431
                     /locus_tag="VIF08_08130"
     CDS             737..2431
                     /locus_tag="VIF08_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY8135990.1"
                     /translation="MTSERRQRFSTISDVEIERLYGPDAWVGPGRGPGGPTAVDHLGD
                     PLRKGAGEWSDFEPGRDIGIPGQPPFTRGIHANGYRARLWTMRMFAGFGSAEDTNARF
                     HQLLKAGQTGLSIAYDMPTLYGYDTDDPEAEGEFGTCGVAVSSLADMEVLLDGLPLDR
                     ISTSMTINSPAAPIWAMYIAAAEKRGFPRDQLEGTTQNDILKEFIAQKEFLFPPTESI
                     RLVTDTIEFGTRELPKWNTISISGYHIREAGSTAVQELAFTIADGMAYVEEALRRGLR
                     VDDFAPRLSFFFNSHSDFFEEIAKFRAARRIWWKLMSSRYRAENERSTWMRFHTQTAG
                     VSLTAQQPLNNLTRVAIQALAAVLGGTQSLHTDSYDEAWAVPSAEAALLALRQQQVIA
                     SETGVTNTVDPLGGSWFVESLTNEVERQVWHYLDEIDALGGMVKAIAAGFPQKEIADA
                     AYRFQREFDNGEREIVGVNVFGDPDEELSIPLLEVSDESKQQHLARLARTRRERDPER
                     VAAALAKLRDLAARPGSSEANLMPALIECANAYATLGEMCGVFRAVFGEYREPVAV"
     gene            2568..>3912
                     /locus_tag="VIF08_08135"
     CDS             2568..>3912
                     /locus_tag="VIF08_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135991.1"
                     /translation="MRSLSLSLRVAAVAAAVSLLLGGSLVPVAAASHPASNPTGMTRF
                     GSLTYFAAETASHGRELWVTDGGPGGTHEVIDINPAGSSNPGSFNVINGILTFVADDG
                     AGPRLWASDGTALGTDKIDTGGVPFSLTDVNGTLFFVQDDMVWISDGTMGGTDPVEAL
                     SASDIGEAVAFKGKYYLLLTKDAPGNDPWWLWKSDGTAEGTHSVRRLVPPGGHFEPEM
                     LVPSGNFLYFILDDQDFAGGEHPASELWRSDGTSAGTKQVIDLNPDYPDSVDDLTNVA
                     GTLFFTADLDGDRELWKSRGTTATTMRVADINASDSSDPGPLTRVGNRLFFGADDGSG
                     RHLWRTKGTMASTVEIDDAAPFECGLFDFACGFVSVNYTPAALGSLYFFPSAAGGKGI
                     ELWVSDGTEPGTFMVKNINPGTAHSRPSDLSPSGSLMYFAAKDGSHGRELWVTNGT"
BASE COUNT          655 a         1341 c         1281 g          635 t
ORIGIN      
        1 tggcgggccg ccgccgccac cgcggacgac gccggtcacg gcgcgcttcg acaccagcgc
       61 cggcttgcgg ccgcgcaaca gcaggacggg ggcgccgccg gccaccatgc gcggcatgta
      121 ccccagggcg cggcgcgact ctggtggcga gcgcgacctg cgtgccagca ccccgaccgg
      181 gccggtgcat cggcgcaccg gtcgtcgcgc ggagccgatg cccaccctgc aggagtcgcg
      241 cgagacgacc ctcaacttcg gccgcttcca cggctacacg ctgggcgagg tggagctgct
      301 cgagccgtcc tacctcgact gggtcgcccg gacgatcacg cgcgatcgcg acttggtcgt
      361 caaggcgcgg ctgatccagc aggatctcga cgagcgcggt gtggcgcggc catcgcgcgc
      421 cgccacccct ggcttcggac caaccttcga cggatgaagt acgagtaccg ataagttcgt
      481 ccgaggccgg cccacccgtt actcggctcg ttgctcgcgg cggcgaggct cccattgcgg
      541 ccccagccgc ccccacaacg cggaaccacg cgtttcgtcg acgagcccgg tggccgacgc
      601 cccggacgcc attgctggct ccacgaacgg tgaaccaact cgcgcgcccc aaccatcatg
      661 cctgcgtacc gcacaccgcc ctgacccata ggtggtgcgc gaccgcgccg tgacacgcct
      721 ttgttagagt cgaaacatga ccagtgagcg caggcagcgc ttcagcacca tcagcgacgt
      781 cgagatcgag cgcctgtatg gccccgacgc gtgggtcggc ccagggcgcg gccccggtgg
      841 tccgactgcg gtcgatcatc tgggcgatcc attgcgcaag ggtgcagggg agtggtcgga
      901 tttcgagccc gggcgggata tcgggattcc gggccagccg cccttcaccc gcggcatcca
      961 cgccaacggc tatcgcgccc gcctgtggac gatgcgcatg ttcgccggct tcggcagcgc
     1021 cgaggacacc aacgcccgat ttcaccagct cctgaaggcc ggccagacgg ggctgtcgat
     1081 cgcgtacgac atgcccacgc tgtacggcta cgacacagac gatccggagg ctgagggcga
     1141 gttcggcacg tgcggcgtcg cggtttcctc gctggctgac atggaggtcc tgctcgacgg
     1201 cctgcccctt gatcgcatct cgacttcgat gacgatcaat tcgcctgccg cgccgatctg
     1261 ggcgatgtac atcgccgcag cggagaagcg cggcttcccg cgcgaccagc tcgagggcac
     1321 gacccagaac gacatcctga aagagttcat cgcccagaag gagttcctct tcccgccgac
     1381 cgagtcgatc cgcttagtga cggacacgat cgagttcggt acgcgcgagc tgcccaagtg
     1441 gaacaccatc tcgatcagcg gctaccacat ccgcgaagcc ggctcgaccg cggtccaaga
     1501 gctcgccttc accatcgccg acggcatggc ctacgtcgag gaagccctgc gccgcggcct
     1561 gcgcgtggat gatttcgcgc cgcggctgag cttcttcttc aactcgcaca gcgacttctt
     1621 cgaggagatc gccaagttcc gtgccgcgcg gcggatctgg tggaagctga tgagctcgcg
     1681 ctaccgcgcg gagaacgagc gctcgacctg gatgcgcttc cacacccaga ccgcgggcgt
     1741 gtcgctgacc gcgcagcagc cgctcaataa cctgacgcgc gtcgcgatcc aggcgctcgc
     1801 cgcggttctg ggtggcacgc aatcgctcca taccgattca tacgacgagg cctgggcggt
     1861 gccctcagcc gaagccgcgc tgctcgccct gcgccagcag caggtgatcg cgtcggagac
     1921 cggcgtgacc aatactgtcg atccgctggg cggctcgtgg ttcgtggagt cgctgaccaa
     1981 cgaggtcgag cgccaggtct ggcactacct cgacgagatc gacgccctgg gcggcatggt
     2041 caaggcgatc gcggctgggt tcccccagaa ggagatcgcc gacgccgctt atcgcttcca
     2101 gcgcgagttc gacaacggcg agcgcgagat cgtgggcgtc aacgtcttcg gcgatccgga
     2161 cgaggagctg tcgatcccgt tgctcgaggt gagcgatgag tcgaagcaac agcatctggc
     2221 ccgtctcgcc cggacacgcc gcgagcgcga tccggagcgc gtcgccgctg ccctggcgaa
     2281 gctgcgtgac ctcgctgcgc gccccggctc gtcggaggcc aacttgatgc ccgccctgat
     2341 cgagtgcgcc aacgcatacg cgaccctggg cgagatgtgc ggcgtcttcc gcgccgtctt
     2401 cggcgagtac cgcgagccgg tcgcggtctg atcgtgccgc ggacgcgggc ctagcccgcg
     2461 gcgccgttga caggcgggcg tacggttacg cgctaagggg ctggctcgtc acccgtgggg
     2521 ggacccggga tccgcgagcg ccgcgccaac ccaggaaggt tgctgcgatg cgctccctgt
     2581 cgctctcgct acgcgttgcc gccgttgcgg ctgctgtatc cctgttgctg ggtggctcgc
     2641 tcgtgcctgt cgcggccgca tcccaccccg cctccaatcc aaccgggatg acccggttcg
     2701 gctcgctgac ctacttcgcc gccgagacgg ccagccacgg ccgcgagctg tgggtcacgg
     2761 acggcggccc aggcggtaca cacgaggtca tagacatcaa tcccgccgga tcgtcgaatc
     2821 caggctcgtt caacgtgatc aacgggatac tgacgttcgt tgccgatgac ggcgctgggc
     2881 cacgcctgtg ggccagcgat gggaccgcgc tcggcaccga caagattgat accggcggcg
     2941 tgccgtttag tctcaccgac gtgaacggga ccctgttctt cgtccaggac gacatggtct
     3001 ggatcagtga cggaacgatg ggcgggacag accccgtcga ggccctctct gcgagcgaca
     3061 tcggtgaggc agtcgccttc aagggcaagt actacctcct tctcacgaaa gacgcaccag
     3121 gcaacgaccc gtggtggctg tggaagagcg acggcacagc cgagggcacg cattcggtca
     3181 ggagactggt accgcccggc ggtcacttcg aacctgagat gctcgtccca tcgggcaatt
     3241 tcctgtactt catccttgac gatcaggact tcgctggcgg tgagcatccc gcttccgaac
     3301 tgtggcgatc ggacggtacg agcgcgggga caaagcaggt gatcgacctg aacccggact
     3361 atccggacag cgtcgatgac ctcaccaatg tcgccgggac gctcttcttc acggcggatc
     3421 tcgacggcga tcgtgagctg tggaagtcga ggggcacgac cgcgaccacg atgcgagttg
     3481 ccgatatcaa cgcgagtgat tcatccgatc cgggtccgct gaccagagtt ggcaacaggc
     3541 tcttcttcgg tgccgacgac ggtagcggcc gccacctctg gcgcacaaag ggcaccatgg
     3601 cgagcaccgt cgagatcgac gacgccgcgc cgttcgaatg tgggctattc gacttcgctt
     3661 gcggcttcgt ctccgtcaac tacacgccgg ccgcgctcgg ctcgctctat ttcttccctt
     3721 cggccgcggg cggcaagggc atcgagctat gggtgagtga cggaaccgag ccgggtactt
     3781 tcatggtcaa gaacatcaat cccgggacgg cgcactcgcg accgtcggat ctctcaccct
     3841 ccgggagcct gatgtatttc gcggcgaaag acggcagcca cggccgcgag ctgtgggtga
     3901 ccaacgggac ga
//
LOCUS       DATBRX010000213        10852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1204507, whole genome shotgun sequence.
ACCESSION   DATBRX010000213 DATBRX010000000
VERSION     DATBRX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10852
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204507"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VIF08_08140"
     CDS             <1..372
                     /locus_tag="VIF08_08140"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEY8135992.1"
                     /translation="AFLLVVPFQQGFSSVSEVARVAYFAGLASCAAASAFLIAPATYH
                     RINLRRGVDEKEQMLFTSSRFAIVGTVFLAAGIVCSMFVVADVLFGELAGIAVAVVTA
                     AVLGGLWYALPISRRRRSRPA"
     gene            519..986
                     /locus_tag="VIF08_08145"
     CDS             519..986
                     /locus_tag="VIF08_08145"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY8135993.1"
                     /translation="MQVGAVWLHTVAFVIAWGFYGILARMILPGLGKTLDLAGQTRTL
                     AAIERRALPFVLLAVVLFVVTGTYLLVVNPRYDGLGSFTNSWATLMLVKHLLVVVLVG
                     LGVLIDYLIRGAADAQDDRDRKSDLRWIGLAADGATGLGALIALTTAAAQLAV"
     gene            complement(1483..1557)
                     /locus_tag="VIF08_08150"
     tRNA            complement(1483..1557)
                     /locus_tag="VIF08_08150"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1523..1525),aa:Gln,seq:ctg)
     gene            complement(1837..2040)
                     /locus_tag="VIF08_08155"
     CDS             complement(1837..2040)
                     /locus_tag="VIF08_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135994.1"
                     /translation="MARCAHCGAVATWRDSRFARQYEHIKESYEERGRSEDKAEEIAA
                     RTVNKERAEHGETKAQKERAGRR"
     gene            2124..2591
                     /locus_tag="VIF08_08160"
     CDS             2124..2591
                     /locus_tag="VIF08_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8135995.1"
                     /translation="MDVLTLLKEDHDRVKRLLEEGDKTTERGEKTRTELFQRLKDMLT
                     AHEAMEEEVLYPALKAHPKAKELTLEAYEEHHVVDMVLEELEMTPVGDEQWGAKFTVA
                     KENIEHHIEEEEGEMFRNIRELFSTDEREAMAAKMAEIQALAKQVQQESAAAG"
     gene            complement(2608..5145)
                     /locus_tag="VIF08_08165"
     CDS             complement(2608..5145)
                     /locus_tag="VIF08_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8135996.1"
                     /translation="MRAKAPALASHDEGLPRSTTEQLVAQQATLAHIGQIALSNRSLA
                     DLFKDSCALVGQVLDTELVALLELSADGDTLHLIEGVGWKPGVVGELNVYSIEHTQSG
                     YTIATGGPVIVPDLATEERFKVWPAVAAHGAKAGMSVRIGGADKPFGALSAYTTRAGR
                     FTRDEANFLQSVANVIAAAVERQRIEEELRASRDQLAAIVSNIDEGITVLTPTGLLFA
                     NDAAAQLSGFETGAEMAATPPRDIVSRFEMFREDGTALPASELPSRRAMAGEEAPEAI
                     VGFRIKATGEERWSAVRATAVRDPSGGRTHVVSTFRDITSERWASHAREFMVDATAAM
                     SGTLDIVEAARRLAELAVPRLADYCSVDLLEADGSVSSVALAHADPARMPLVEEARSL
                     QHVATDAPSGPGKVIREGTPELVQLSLEMLETAGATEQAIRLFKGLELRSYLCVPLMG
                     RHGPIGALTLVMAQSGRNLGPRELALAVELGQRAGIALENAQLYRTANDRRAQLDIVL
                     AALEEAVIVYDGGGKLRLGNRAAAGIFEGTLPTTLDELWRRLTPLPGGPKPDKARAEM
                     GVEVAPDGGDRWFELRRYGAPIGPAAESAAATLPPPTVVVLRDVTQARAERAAREAFL
                     GVLSHELRTPITTIYGGSQLLERGLDRGRRTEVITDIRVESERLVRLVEDLLVMTRVE
                     RGIVETSEEPVLLQHLLAKIIAGSPARWGGAHINLHLAPRLPAVRGDATYIEQVVRNL
                     LTNAVRYGRAAEKGIDVHAEETDHSVAVRVQDHGDGLDGEDPNRLFELFYRSPSARSV
                     PGGAGIGLFVTRSLVEAMGGRIWATDRPDGGAEFGFTLPVLEPDAVL"
     gene            complement(5280..8027)
                     /gene="ligD"
                     /locus_tag="VIF08_08170"
     CDS             complement(5280..8027)
                     /gene="ligD"
                     /locus_tag="VIF08_08170"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY8135997.1"
                     /translation="MSLDTYKKKRDFRKTPEPAGEVGAPPRGSRRYVVQRHRATRLHY
                     DFRLEIDGVLMSWAVPRGPSLNPADRRMAVHVEDHPLDYFDFEGVIPKGEYGGGDVII
                     WDWGTFEAEETDDPGQAVKSGELKFALDGEKLHGRFTLVKTRGSKSGENDWLLIHKKD
                     EFADPEWDVDALPRSVKTGRTNDEVKAGRDAIWDSGAPAAEAAIVLAGARAARLPAFI
                     EPMAATLAGGPFDDPDWIFELKLDGYRIEAVVDNGKVKLWTRNQQDGARYFPDLAGAK
                     PTWIKAEQAIVDGEVVALDRTGRPTFSLLQDRAGMGRFGPRPVPVAKESRRGKKAVAE
                     EPDDGFVAPVVYYVFDILYHDGRLLTEVPLEQRKRLLRSLLREHDSVRYAAHFDEGKQ
                     FYEVVRQQGLEGLIAKLRTSRYEAGRRSKAWLKIKIRREQEVVVIGYELGKGARADLG
                     SLILAVRDGNEWSYVGEVGSGLDNRTIRQLKKELDDHAIDSPVAPNAPRIRGARWSEP
                     RLVVRVEFSEWTDDGLLRQSAYKGIEIGKDPKSVRREVGIATGPAVKAAEREAEKGAA
                     APPKRAAPKPAELKVAPTPAAPKPKARAKKSAAARPPQSATKQELAALDAMTRDGLWE
                     IGGESVSLTNLDKVLFPEPGFTKRDLIRYYVSVAPVLMPHLAGRALNLSRWPDGVTGH
                     SFWQKQIPDWAPDWIARWDYPEAGSSESHTYLVADRVATMAWLANHATIDLHPWTSRT
                     DSYRSPTFALIDIDPGEKTTFADILTLARLYRAALDHLGVLGLPKTTGKRGIQIWVPV
                     RPGYTYRETSGWVEALSRAIGAAAPSLVSWEWAKRSRRGLARLDFTQNAVNKTLVAPY
                     AVRPVANAAVSTPITWDELDDPGLRPDRWNIKTVLERIKKRGDLFAPALSLEQELPEL
                     G"
     gene            complement(8063..8548)
                     /locus_tag="VIF08_08175"
     CDS             complement(8063..8548)
                     /locus_tag="VIF08_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135998.1"
                     /translation="MRNRIVGYGPMTPRVMAFGAGGVDRNQGSYGSPGGSDTRGARTM
                     IEPLMRFSLEVEADSLRLEPEYREGDRNSRTLAKETDLRVMLTVLREGAALDEQDGDA
                     RTTVQVIDGTATLEIANAEAHLEPAEVAVIDRGQPWIIRAESDCALLLTIAWPPEKAG
                     V"
     gene            complement(8551..8769)
                     /locus_tag="VIF08_08180"
     CDS             complement(8551..8769)
                     /locus_tag="VIF08_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8135999.1"
                     /translation="MDEERKLPGEPGEGAHQPGPTDDPDAGDLRRLGEEGGPVGLRVG
                     RNPANLGDEVPEAGDVTLPETVPATGEM"
     gene            complement(8846..9937)
                     /locus_tag="VIF08_08185"
     CDS             complement(8846..9937)
                     /locus_tag="VIF08_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8136000.1"
                     /translation="MRAEAFRWFVRGVGLAFGAAVAVGVIYGLLLSARVAVLVFIALL
                     LASGLEPLIDRLRARGGLGRGATLLLVYAGFFGLVGVVALLVIPGAISQFGELGARIT
                     PLLTSVREWARSVEPRALSVSLVGLVDTVQGVLVPAVPSAPEPDQLIALGLTFAEIVI
                     SAIAVLTMVYFWLTERARLQRFFLALLPADRRGGAREAWNEMELRLGGWVRAQLILMG
                     SVGVATTIAYFLIGLEGALLLGLIAALAEAIPLVGPALGAIPALIVAAMDGRIEVVIL
                     VAVVYVVIQIVEGNILVPFVMHNTIGVPPFLVVISILAGAAIAGIPGALLSVPLAAAL
                     LVVVERLQARSETVPLSPAQPATGEPVEI"
     gene            10086..>10852
                     /locus_tag="VIF08_08190"
     CDS             10086..>10852
                     /locus_tag="VIF08_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8136001.1"
                     /translation="MNSQPLVLVADDEPRITKLVALTLHDEGFRVVTAGGGEEALKKA
                     EEVRPDIVLLDIVMPDLDGIEVMRQLREWRPVPVILLTAKGSTSDKAKGLDLGADDYV
                     AKPFHPDELAARVRAVLRRASGVSPGAGIVAFDDIEIDLERRLLRKGGELVSLSRTEW
                     LLLQHLAAHAGKVVLHTELLTKVWGPEYRDDLQYLRVWISRVRRKLGAAPGEAGPIKT
                     FQGIGYVLDTEGNLGDSSTSHGGGTATGSTGPATGTAV"
BASE COUNT         1675 a         3784 c         3620 g         1773 t
ORIGIN      
        1 gcattcctgc tggtcgtgcc cttccagcag ggcttctcgt cggtcagcga agtcgcgcgc
       61 gtggcctatt tcgccgggct cgcgtcctgc gcggcggcga gcgccttcct catcgcgccg
      121 gccacgtacc accgcatcaa cctgcgccgc ggcgtcgacg aaaaggagca gatgctcttt
      181 acctcgagcc ggttcgcgat cgtgggcacg gtcttcctcg ccgcgggcat cgtgtgctcg
      241 atgttcgtgg tcgccgacgt gctgttcggc gagctggccg gaatcgcggt ggcggtcgtc
      301 acagccgcgg tgctgggtgg gctctggtac gcgctgccga tctcgaggcg gaggcgcagc
      361 cgcccggcct gacgccatgt cttctcgtcg cggcctgacg ctctgccagg cgacgctcac
      421 ggccgaccgg accgccgcag cctgacctcc ggccctacca ctggggcgct gaattcggga
      481 tcgtcacatc ccggaggagg aggcagcaag tggatttgat gcaggtcggc gccgtgtggc
      541 tgcacacggt cgcattcgtg attgcgtggg gtttctacgg gattctcgcg cggatgatcc
      601 tgcctggact gggcaagaca cttgatctgg cgggacagac gcgaaccctg gccgcgatcg
      661 agcgccgcgc gttgcccttc gtcctgctgg ccgtggtgct gttcgtcgtc acgggcacgt
      721 acctgctcgt ggtcaacccg cgctacgacg gactcggcag cttcaccaac tcgtgggcga
      781 cgctgatgct ggtcaagcat ctgctggtcg ttgtcctggt cggcctgggc gtgctcatcg
      841 attacctgat ccgtggcgcg gccgacgcgc aggacgatcg cgaccgcaag tccgacctgc
      901 gctggatcgg cttggccgcg gatggcgcga cggggctggg ggcgctcatc gcgctcacga
      961 ccgcggccgc acagctagct gtctagaagg agccagacat gcgcaagttg agatggtcac
     1021 tggcgatcgt cgccgttgtc gtggagatcg ccgcgctcgc agtgtggcta tggacgcgca
     1081 accgccgaat aggcacgaag ctcatgaacg agaccgtgaa ccggttcctg ctccggcgcg
     1141 gcctggccgg cggccggaca tctgagatcg ccacgctcga gcacttcggg cgcaaatccg
     1201 ggacacgccg cctgacgctc atccatcccg aaccgactgc ggacggcatc cggatcatgg
     1261 cgccggttgg cgagcagtca gagtgggcgc gcaacgtcct ggccgcgggg cactgccgga
     1321 tccagtggca cggtcggatc gaagagttcg acgagccgcg catggtcgct ccgacgatgc
     1381 tgaccgactt gccgccgctg gtccgccgcg tgaccgacgc actgggcttt cgctacgtgc
     1441 tgctccatcg gttcgcgcag tccgagggcg tactggcaga agtggctggc gaggaaggat
     1501 tcgaaccttc aatcccctga tccagagtca ggtgccttac cgtttggcca ctcgccagtg
     1561 acgccctgag tctaccaagc ggaccgcccc aagcccgatc tcgcggcagc cggaccgctc
     1621 acccggcaga tgtgccgctc agtcagcctt aagccgacgc ggcgcgagtt gcgccctcca
     1681 acccacgcgt ggctgagcgt gaaatcgtgg ccgccgccgg agatttcaga gtcccaggcc
     1741 gcctaacgct gatcagcgat gacaagtgct gggaagcagc gaccgggcgg ccgcaccccg
     1801 tggcgcaaac aggcacccac cgctgcccgc ccggaactag cggcggccgg cacgctcctt
     1861 ctgggctttc gtctccccgt gctcggcgcg ttccttgttg acggtccgcg cggcgatctc
     1921 ctcggccttg tcctccgatc gcccgcgctc ctcgtacgac tccttgatgt gctcgtactg
     1981 gcgcgcgaat cgtgaatcgc gccacgttgc aaccgcgccg caatgagcgc agcgggccac
     2041 cgactgaccg taacggaggc ccattgcgta cggtcgggcc ctcatcaccg acatcaccga
     2101 ctgtcactca aaggaggctg gccttggacg tacttaccct gctcaaggaa gaccacgacc
     2161 gcgtgaagcg cctgctcgag gaaggcgaca agaccacgga gcgcggcgag aagacccgaa
     2221 ccgagctgtt ccagcggctc aaggacatgc tgacagcgca cgaggcgatg gaggaggaag
     2281 tcctctaccc ggcgctcaag gcgcatccca aggccaagga gctgacgctg gaggcgtacg
     2341 aggagcacca cgtcgtcgac atggtcctcg aggagctcga gatgacgccg gtcggcgacg
     2401 agcagtgggg cgccaagttc accgtcgcca aggagaacat cgagcaccac atcgaggagg
     2461 aagagggcga gatgttccgc aacatccgcg agctcttctc gaccgacgag cgcgaagcga
     2521 tggccgccaa gatggcggag atccaggccc tcgccaagca ggtgcagcag gaatctgcgg
     2581 cggccggctg atcgggatca ggctcggcta gaggaccgcg tcgggctcca gcacaggcag
     2641 ggtaaagccg aattccgcgc cgccatcagg cctgtcggtg gcccagatgc ggccgcccat
     2701 cgcctctacc aggctgcgcg tcacgaagag tcctatcccc gcgccgcctg ggaccgagcg
     2761 cgcgctgggg ctgcggtaga acagctcgaa cagccggttg gggtcctcgc cgtcgagtcc
     2821 atcaccatga tcctggacgc gcaccgcgac cgagtggtcg gtctcttcgg cgtgcacgtc
     2881 gatgcccttc tcggccgcgc ggccgtagcg gacggcgttc gtcagcaggt tgcgcacgac
     2941 ctgctcgatg taggtcgcgt cgccgcgcac ggcgggtagc cgcggcgcaa ggtgcaggtt
     3001 gatatgggcg ccgccccagc gcgccggcga gccggcgatg atcttcgcga gcagatgctg
     3061 gagcaggacc ggctcctcgc tcgtctcgac gatcccgcgt tcgacgcgcg tcatcacgag
     3121 caggtcctcg accaaacgca cgagccgctc ggattcgacg cggatgtcgg tgatcacctc
     3181 tgtgcgccgg ccacgatcga ggccgcgctc caagagctgg ctgccgccgt agatggtggt
     3241 gatgggcgtg cgcagctcat gcgacagcac cccgaggaat gcctcgcgcg ccgcgcgctc
     3301 ggcgcgcgcc tgggtcacgt cgcgcaacac gacaaccgtg ggtggcggca gcgtcgcagc
     3361 ggctgattcg gcggccgggc cgatcggcgc gccatagcgc cgcagctcga accagcggtc
     3421 gccgccgtcg ggtgcgacct cgacgcccat ttccgcacgg gctttatccg gctttggtcc
     3481 gcccggcagt ggagtgaggc gccgccacag ctcgtcgagc gtggtcggca gggtgccctc
     3541 gaagatgccc gcggcggcgc gattgcccag gcggagcttt ccgccgccgt cgtagacgat
     3601 gaccgcttcc tcgagcgcgg cgaggacaat gtcgagctgc gctcgccggt cgttggccgt
     3661 gcggtagagc tgtgcgttct cgagggcgat accggcgcgc tggcccagct cgaccgccag
     3721 cgcgagctcg cgcggaccaa ggttgcgccc cgactgggcc atcacgagcg tcagcgcccc
     3781 gatcggcccg tgccggccca tcagcggcac gcataggtat gaacgcagct ccaggccctt
     3841 gaacagccgt atcgcttgct cggtcgcgcc cgcagtctcg agcatctcca gggagagctg
     3901 gaccagttcg ggcgtgcctt cgcggatgac cttgcccggt ccgctggggg cgtcggtcgc
     3961 gacatgctgc aacgatcgcg cctcttccac cagcggcatt cgggccgggt ccgcgtgcgc
     4021 gagggccacg gacgagacgc tgccatcggc ttcgagcagg tccacgctgc agtagtcggc
     4081 cagccgcggc acggccagct cggccagccg gcgcgcggcc tccactatgt cgagtgtgcc
     4141 cgacatcgcg gccgtggcgt caaccatgaa ttcgcgcgcg tggctcgccc agcgctcaga
     4201 cgtgatgtcg cggaacgtgc tcacgacgtg cgtccgcccc ccggaggggt cgcgcaccgc
     4261 ggtggcgcgc accgccgacc agcgttcctc gccagtcgcc ttgatcctga accccacgat
     4321 cgcctccggc gcctcctcgc ccgccatcgc gcgccggctg ggcagttccg acgcgggcag
     4381 cgccgtgccg tcctcgcgga acatctcgaa cctgctcact atgtcgcgcg ggggcgtggc
     4441 ggccatctcc gcccccgtct cgaagcccga cagctgtgcc gccgcgtcgt tggcaaagag
     4501 caggccggtc ggcgtgagga ccgtgatgcc ctcgtcgatg ttgctcacga ttgccgcgag
     4561 ctggtcgcgc gacgcgcgca gctcctcctc gatgcgctgg cgctcaaccg cggccgcgat
     4621 cacgttggcc acggactgca ggaagttcgc ctcgtcgcgc gtgaatcgcc cggcgcgcgt
     4681 ggtgtacgcg gacagcgcgc cgaacggctt atcggcaccg ccgatgcgca cgctcatgcc
     4741 ggccttggca ccgtgcgcgg cgaccgccgg ccaaaccttg aaccgctcct cggtcgcgag
     4801 gtcgggcacg atcaccggcc caccagtcgc gatcgtgtag cccgactgcg tgtgctcgat
     4861 cgagtacacg ttgagctcgc ccacgacgcc cggcttccag ccaacgcctt cgatgagatg
     4921 cagtgtgtcg ccatctgccg acagttccag cagggccacc agctccgtgt cgagcacctg
     4981 accgaccagg gcgcaggagt ccttgaagag atcggccagc gagcgatttg acagggctat
     5041 ctggccaatg tgcgccaagg tcgcctgctg ggcgaccagt tgctctgtcg tggaccgggg
     5101 caggccctcg tcgtggctcg ccagggccgg cgccttcgcg cgcactctcc ctggatcctt
     5161 gtgcgcagcc aaacgtgatc ccctcgccat acgacagccg agaatatccg cgccttggcg
     5221 ccggggcgat gtacgcggca taacagtttg cccggtgagc ccggccggcg ccggccggat
     5281 cagcccagtt cgggcagctc ctgttccagg ctgagcgccg gcgcgaagag atcgccgcgc
     5341 ttcttgatcc gctcgaggac cgtcttgatg ttccacctat cgggccgcag cccagggtcg
     5401 tcgagctcgt cccaggtaat gggcgtcgaa acggccgcgt tggcgaccgg gcgcacggcg
     5461 tagggcgcga cgagcgtctt gttgacggcg ttctgggtga agtccagccg cgccaggccg
     5521 cgccgcgaac gcttggccca ctcccagctc acgaggctcg gggcggctgc gcctatggcg
     5581 cgcgacagcg cctccaccca gccgctcgtc tcgcgatacg tgtagccggg ccgcaccggc
     5641 acccagatct ggatgccgcg cttgccggtc gtcttgggca gcccgagcac cccgaggtgg
     5701 tcgagcgccg cgcgatacag ccgggccagg gtcaggatgt cggcgaaggt ggtcttctcg
     5761 ccggggtcga tatcgatcaa cgcgaacgtt gggctgcgat acgaatcggt cctggacgtc
     5821 cagggatgaa gatcgatcgt cgcgtgattg gccagccagg ccatcgtcgc gacgcggtcc
     5881 gcgacaaggt aggtgtggct ctcgctcgag ccggcctccg ggtaatccca gcgcgcgatc
     5941 cagtcgggcg cccagtcagg gatctgtttc tgccagaacg agtggccggt cacgccgtcg
     6001 ggccaacgcg agaggttgag tgctcggccg gccaggtgcg gcattagcac cggcgcgacg
     6061 ctgacgtagt agcggatcag gtcgcgcttg gtgaaaccgg gctccggaaa gagcaccttg
     6121 tcgaggttgg tcaggcttac cgattcaccg ccgatctccc acaggccgtc gcgggtcatg
     6181 gcgtcgaggg cagccagctc ctgtttggtc gcgctctgtg gaggtcgagc cgcggccgac
     6241 ttcttcgccc gcgccttcgg cttgggcgcg gccggtgtcg gtgccacttt aagctcggca
     6301 ggtttcggcg cggcccgctt cggcggagcc gccgcgccct tttcagcctc cctctccgcc
     6361 gccttcacgg ccgggccagt cgctatgccc acctcgcgcc ggacggactt ggggtccttg
     6421 ccgatctcga tgcccttgta ggccgactgg cgcaacagcc cgtcgtccgt ccactcgctg
     6481 aactccaccc ggacgaccag gcgcggctcg gaccagcgcg ccccgcggat gcgcggcgcg
     6541 ttcggggcga ctggcgaatc gatggcgtgg tcgtcgagct ccttcttgag ctggcggatg
     6601 gtcctgttgt cgagaccgct gccgacctcg cccacataac tccactcgtt gccgtcgcgc
     6661 acggcgagga tgagcgagcc gagatctgct cgagcgccct tgcccagctc atagccgatg
     6721 accactacct cctgctcgcg ccggatcttg atcttgagcc acgccttgct gcgccggccc
     6781 gcctcgtagc ggctcgtgcg caacttggcg atgaggcctt ccaggccctg ctggcgcact
     6841 acttcgtaga actgcttgcc ctcgtcgaag tgcgcggcgt agcgaaccga gtcgtgctcg
     6901 cgcagcagtg agcgcaggag ccgcttgcgc tgctccagtg gcacttcggt cagcaggcgg
     6961 ccatcgtggt agaggatgtc gaacacgtag tagacgaccg gggcgacgaa gccgtcgtcc
     7021 ggttcctccg cgaccgcctt cttgccccgc cgcgactcct tggctacggg caccggtcgc
     7081 gggccgaagc ggcccatgcc cgccctgtcc tggagcagcg agaatgtcgg ccggccggtt
     7141 cgatcgagcg ccacgacctc gccgtcgacg atggcctgct cggctttgat ccaggtcggc
     7201 ttggcgcctg cgaggtcggg gaagtagcgc gcgccgtcct gctggttgcg cgtccacagc
     7261 ttgaccttgc cgttgtcgac gaccgcctcg atccggtagc cgtcgagctt cagctcgaag
     7321 atccaatcgg gatcgtcgaa cggaccgccg gccagcgtcg ctgccatggg ctcgatgaac
     7381 gcgggcagcc tggccgcgcg cgcaccggcc agaacgatgg ccgcctcagc tgccggcgcg
     7441 cccgaatccc agatcgcgtc gcggcccgcc ttgacctcgt cattggtccg ccccgtcttg
     7501 acggagcgcg gcagcgcatc gacgtcccac tcaggatcgg cgaactcgtc cttcttgtgg
     7561 atcagcagcc aatcgttctc acctgacttc gaaccgcgcg tcttgaccag ggtgaagcgg
     7621 ccgtggagct tctcgccatc gagggcgaac ttgagctcac ccgacttgac cgcctggcca
     7681 ggatcgtcgg tctcctcagc ctcgaacgtg ccccagtccc agatgatcac gtcgccgccg
     7741 ccgtactcac ccttggggat cacgccctcg aaatcgaagt agtcgagcgg gtgatcctcg
     7801 acgtggaccg ccatgcgccg atccgccggg ttgagcgacg ggccacgcgg cacggcccac
     7861 gacatcagca cgccgtcgat ctcgagccgg aagtcgtaat gcaggcgggt ggcgcggtgg
     7921 cgctggacca cgtagcgccg cgacccgcgc ggcggtgcgc cgacctcacc ggccggttcc
     7981 ggggtcttcc tgaagtcgcg cttcttcttg taggtgtcga gcgacatcgc gccgctcagg
     8041 ttacggcgtc actcgccgcg gatcagacgc ccgccttctc tggtggccag gcgatcgtca
     8101 gcagcagcgc acagtcactc tcggcgcgga tgatccacgg ctggccgcga tcgatgaccg
     8161 ctacctcggc cggttccagg tgtgcctcgg cgttggcgat ctcgagtgtc gctgtgccgt
     8221 cgatgacctg caccgtcgtg cgcgcgtcgc cgtcctgctc atcgagcgcg gcgccctcgc
     8281 gcagcacggt gagcattacg cgcagatcgg tctccttggc cagcgtgcgc gagttgcgat
     8341 cgccctcgcg gtactcgggt tcgagtctga gggagtcagc ctcgacttcc agagaaaacc
     8401 gcatgagcgg ctcgatcatc gtccgtgccc cgcgcgtatc gctgccgccc ggactgccat
     8461 aggagccctg attgcgatcg accccgccag cgccaaacgc catgacccgc ggagtcattg
     8521 gcccgtagcc gacgatccga ttgcgcatgc tcacatctca ccggtcgccg ggaccgtctc
     8581 gggcagggtc acgtcgcctg cctcgggcac ctcgtcgccc aggttggcgg ggttgcgccc
     8641 cactcggaga cccaccgggc cgccttcttc ccccagccgg cgcaggtccc ccgcgtcagg
     8701 atcgtcggtt gggccgggtt gatgcgcacc ctcgcccggc tcgcctggaa gcttgcgttc
     8761 ttcgtccatt gcgtcagcgt ggtggtcagc cagttgcgcg caggctcaag tgccgtgcgt
     8821 cggtgcctgt caggtgcctt tcaactcaga tttcgacagg ttcgcccgtt gccggctggg
     8881 cgggcgacag cgggacggtc tccgatcgcg cctggagacg ctctacgaca acgagcagtg
     8941 cggcagcgag cggcacggac agcaacgccc cgggtatgcc cgcgatggcc gcgccggcaa
     9001 ggatgctgat cacgaccagg aacggcggaa cgccgatcgt gttgtgcatc acgaacggca
     9061 cgaggatgtt gccctcgaca atctgaatga cgacgtagac gacggcgacg aggatcacca
     9121 cttcgatccg cccgtccatc gcggcgacga tcagggccgg tatggcgccc agcgccggcc
     9181 cgacgagtgg tattgcttcg gcgagcgcgg cgatgagccc gagcagcagc gccccctcga
     9241 gcccgatcag gaagtaagcg atcgtcgtcg ccacccccac gctgcccatc aggatgagct
     9301 gcgcgcgcac ccatccgccc agtcggagct ccatttcgtt ccaggcctcg cgggcgccgc
     9361 cgcgcctgtc ggcgggcagc agcgcgagga agaagcgctg tagccgggcg cgctcggtca
     9421 gccagaagta gaccattgtc agcaccgcga tcgcgctgat cacgatctcg gcaaacgtca
     9481 gacccagggc gatgagctgg tccggctccg gcgcgctcgg caccgctggc acgagaaccc
     9541 cctgaaccgt gtcgaccagg ccaacgaggc tgaccgagag cgcacgtggc tcgaccgacc
     9601 gcgcccattc gcgcacgctc gtgagcagcg gcgtgattcg cgcgcccagc tcgccgaatt
     9661 ggctgatggc accggggatg acgagcagtg cgaccacccc cacgagcccg aagaacccgg
     9721 cgtataccag caagagcgtc gccccacggc cgaggccgcc gcgcgcccgc aaccggtcga
     9781 tgagcggttc gagcccggat gccaggagca gcgcgatgaa tacgagcacg gctacccgcg
     9841 cgctcagcag caggccgtag atcacgccca ccgccacggc cgcgccaaag gccaggccaa
     9901 cgccgcgcac gaaccaccgg aacgcttcgg ctctcatctg cgttcaagat tggccgtgcg
     9961 cggccatttt ctcaatcccg ccagttgcaa tactgattgc aatttgtaat gtcatggccg
    10021 cacgcctgta gcgttgcacg cgccgcaggg atctagtgtc ggagccgcaa taggagggct
    10081 cactcttgaa tagccaacca ctggtccttg tggccgacga cgagccgcgt atcaccaagc
    10141 tcgtcgctct gaccctgcac gatgaaggtt tccgcgtcgt caccgccggc ggcggtgaag
    10201 aggcgctcaa gaaggccgag gaagtccggc ctgacatcgt cctgctcgac atcgtgatgc
    10261 ccgatctgga tggcattgaa gtcatgcgcc agctgcgcga gtggcgaccg gtgccggtca
    10321 tcctgctcac ggccaagggc tcgaccagtg acaaggccaa gggccttgac ctgggcgccg
    10381 acgactacgt cgccaagccc ttccaccccg acgagctggc cgcgcgcgtt cgcgccgtgc
    10441 tgcgccgcgc aagcggtgtc tcgcccggcg ccggcatcgt cgcgttcgac gacatcgaga
    10501 tcgacctcga gcgtcggctg ctgcgcaagg gtggcgagct cgtgtcgctc agccgcacgg
    10561 agtggctgct gctgcagcat ctcgccgcgc atgccggcaa ggtcgtgctt cacaccgagc
    10621 tcctgaccaa ggtctgggga cccgagtatc gcgacgacct tcagtacctg cgcgtctgga
    10681 tctcgcgcgt gcgtcgcaag cttggcgcgg cgcccggcga ggccggcccc atcaagacgt
    10741 tccagggcat cggctacgtg ctggacactg agggcaacct cggcgactcg agcaccagcc
    10801 atggcggcgg cacagcgacc ggctcgaccg gcccggccac cggcaccgcg gt
//
LOCUS       DATBRX010000214         4278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1539406, whole genome shotgun sequence.
ACCESSION   DATBRX010000214 DATBRX010000000
VERSION     DATBRX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539406"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VIF08_08195"
     CDS             complement(<1..399)
                     /locus_tag="VIF08_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY8136002.1"
                     /translation="MPKFQAPRGTRDLLPADMSVWRQLEEGARRLAASYGYQPIETPM
                     FEDSAVFERGIGEVTDVVEKELFRVRTPGGDKDPWALRPEPTAGICRAYVEHGMHTLP
                     QPVKLTMFGPMFRYDRPQAGRYRQFYQWDVE"
     gene            418..780
                     /locus_tag="VIF08_08200"
     CDS             418..780
                     /locus_tag="VIF08_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136003.1"
                     /translation="MRDASRAADAAAAADSPPAQRVIVLPFLRRVGGRLIMPGWLAIT
                     IWRWIIAWRPLDEVELAHELAHVRQWKANGWRYIPRYVAESRRAARGGGDRYRDNRFE
                     VEARAAADAVREHSSRAS"
     gene            complement(803..3052)
                     /locus_tag="VIF08_08205"
     CDS             complement(803..3052)
                     /locus_tag="VIF08_08205"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8136004.1"
                     /translation="MSIEPLSRPQERAAGGGPRQRITGALSFGFSRRALNASERKLEE
                     ELAAHYPAADLERVRAAWQFATEAHGAQKRASGEPYVSHPLAVARTLSELGLDPDAIV
                     AALLHDIPEDTDFGLADIEERFGARVAQLVDGVTKLGKLGSSRTTEEQQAENIRKMLL
                     AMAEDIRVVLIKLADRLHNMRTIAALPADKQQRIARQTAEIYAPLAERLGIWQIKWEL
                     EDLSFKVLEPEEYRRLADQLETRRKEREAYVKRAIVVLREALTKAGVIGELSGRPKHI
                     SSIWKKMQRKGASLSEIYDVHAIRVLVDEIPDCYAALGVVHSLWRPIPNQFDDYIAVP
                     KANGYQSLHTAVIAIDGKPLEVQIRTHAMHQVSEMGIAAHWRYKEGGKADRGYDEKLA
                     WVRQLMDWQRDVSDATEFVEGLKVDLFRDQVFVFTPRGDVKDLPAGSTPLDFAYRIHT
                     DVGHSTIGAKVNNRLVPLDYKLNNGDIVEIVTTKAARGPSRDWMDKVVTGHAKDKIRQ
                     WFKRQEREVNIVHGREAMERELRRLARSSLEKIGPERIEAMVDAYKLASVDDFFAAIG
                     YGAISAQQVVARLDVVTTEGATEQVLPQVAPPSAARTDGVRVKGVGDLMIRFANCCHP
                     IPGDAIMGYITRGRGVTVHQVTCPTVVNERELGRLIDVEWEGGAQQTYPIAIRVDAYD
                     RQGLLNEITNVVAEHKVNIIAANVKVNPDHTATIQATLQVASVDQLARVLSRIEQLKD
                     VHAVQRELS"
     gene            3141..>4278
                     /locus_tag="VIF08_08210"
     CDS             3141..>4278
                     /locus_tag="VIF08_08210"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8136005.1"
                     /translation="MSSLLRSISFALALLLAACTSAPTPSATGSPTPTFAPSLSASAT
                     ASPLATPITTPGATPTASPGASATPPAIPTPTPTATPPGTLATRLDAQLRQVLDQQRT
                     TLDIPGISAAITFPDGSTWTTALGMAEVLPARAATTSTPFVVGSVSKTFVAATILQLV
                     DEGALALDDPLSSWLPDYPLADQITLRMLLGHTSGVFNLYESPDYMPLVVDRPHRTWT
                     PQMVLDRLGGAPYCEPGTCYHYSNTGFILLGLVIEAETGQPLGTVWRERFFTPLGMSD
                     TYFQGDGPPPATSALGYRKTKTGNVPINDGSSYRPTESEGTVIWAAGGIVASAGDIAR
                     WGRALYGGDVLSDASLATMTTFTFHPEKQGGYYGMGTRMRKFGAF"
BASE COUNT          621 a         1497 c         1422 g          738 t
ORIGIN      
        1 ctcgacatcc cactggtaga actggcggta acggccggcc tgcgggcggt cgtagcggaa
       61 catcggcccg aacatggtca gcttcaccgg ttgtgggagc gtgtgcatgc cgtgttcgac
      121 gtaggcgcgg cagatgccgg cagtcggctc tggccggagc gcccatggat ccttgtcgcc
      181 gcccggcgtg cggacgcgga agagctcctt ctcgacgacg tcggtcacct cgccgatccc
      241 gcgctcgaag accgcgctgt cctcgaacat gggcgtctcg atgggctgat agccgtacga
      301 cgcggcgagg cggcgcgcgc cctcctcgag ctggcgccag accgacatgt cggccggcag
      361 caggtcacgc gtgccgcgcg gcgcctggaa cttgggcatg gcgctaggtt agcgagcgtg
      421 agggacgctt cgagggccgc cgacgctgcc gctgcagccg attccccgcc tgcccagcgc
      481 gtcatcgtgc tgcccttcct gcgccgcgtc ggtggccggc tgatcatgcc gggctggctc
      541 gcaatcacga tctggcgctg gatcatcgcc tggcgcccgc tcgatgaggt tgagctggcc
      601 cacgagctgg cgcacgttcg ccagtggaag gccaacggct ggcgctacat cccgcgctac
      661 gtcgccgagt cgcgccgcgc ggccaggggc ggcggcgacc gttaccgcga caaccgcttc
      721 gaggtggagg cgcgcgccgc cgcggacgcg gtccgcgagc attcgtcccg cgcgtcgtga
      781 ttttgttctg tcgattccgc gcctagctga gctcgcgctg aacggcatga acatccttga
      841 gctgctcgat gcgcgatagg acacgggcca gctgatcgac cgacgcgacc tggagcgtcg
      901 cctggatggt cgcggtgtga tcggggttga ccttcacgtt cgcggcgatg atgttgacct
      961 tgtgctcggc cacgacgttc gtgatctcgt tgagcaaacc ctggcggtcg tacgcatcga
     1021 cgcggatcgc gatgggatag gtctgctggg cgccaccctc ccactcgacg tcgatcagcc
     1081 ggccgagctc gcgttcgttg accaccgtag ggcaggtcac ctggtgcacg gtcacccccc
     1141 ggccgcgcgt gatgtagccc atgatcgcgt cgcccgggat ggggtggcag cagttggcga
     1201 agcggatcat gagatcgccc acgcccttga cgcgcacacc gtctgtccgc gcggctgacg
     1261 gcggcgcgac ctgcggcaac acctgctcgg tcgcgccctc cgtggtgacg acatcgagcc
     1321 gggccacgac ctgctgtgcc gagatcgcgc cgtagccgat ggccgcgaag aagtcgtcga
     1381 cgctggccag cttgtaggcg tcgaccatcg cctcgatgcg ctcaggcccg atcttctcga
     1441 gtgacgagcg ggcgaggcgg cgcagctcgc gctccattgc ctcgcggccg tggacgatgt
     1501 tgacctcgcg ctcctggcgc ttgaaccact ggcggatctt gtccttggcg tggccggtga
     1561 cgaccttgtc catccagtcc cgcgacgggc cgcgcgccgc cttggtcgtc acgatctcga
     1621 cgatgtcgcc gttgttgagc ttgtagtcga gcggcacgag gcggttgttg accttggcgc
     1681 cgatcgtcga gtggccgaca tcggtatgga tgcgatacgc gaagtcgagc ggagtggaac
     1741 ctgcggggag atccttgaca tcgccgcgcg gcgtgaagac gaacacctgg tcccggaaca
     1801 ggtcgacctt gagaccttcg acgaactcgg tcgcgtcgga cacgtcgcgc tgccagtcca
     1861 tgagctggcg cacccaggcg agcttctcgt cgtagccgcg gtcggccttg ccgccttcct
     1921 tgtagcgcca gtgggcagcg atgcccatct cgctcacctg gtgcatggcg tgggtgcgga
     1981 tctggacctc gagcggcttg ccatcgatcg cgatcaccgc ggtgtggagc gattggtagc
     2041 cgttcgcctt gggcaccgcg atgtagtcgt cgaactggtt gggaatcggc cgccacagcg
     2101 aatgcacaac gccgagcgcg gcgtagcaat cgggaatctc gtcgacgagc acgcggatcg
     2161 cgtggacgtc gtagatctcg gacagcgacg cgcccttgcg ctgcatcttt ttccagatgc
     2221 tgctgatgtg ctttggccgg ccgctgagct cgccaatgac gccggccttg gtcagggcct
     2281 cgcgcagcac cacgatcgcg cgctttacgt aggcctcgcg ttccttgcgc cgcgtctcga
     2341 gctgatcggc gaggcgccga tactcctcgg gttcgagcac cttgaacgag agatcctcga
     2401 gctcccactt gatctgccag atgcccaggc gctcggccag cggggcgtag atctccgcgg
     2461 tctggcgggc gatgcgctgc tgcttgtcag ccggcagcgc ggcgatcgtg cgcatgttgt
     2521 ggagccggtc ggcgagcttg atgaggacga cgcggatgtc ctccgccatc gcgaggagca
     2581 tcttgcggat gttctcggcc tgctgctcct cggtcgttct cgacgagccg agcttgccca
     2641 gcttggtgac gccgtcgacg agctgcgcga cgcgcgcgcc gaagcgctcc tcgatgtccg
     2701 ccaggccgaa gtcggtgtcc tctggaatgt cgtgcagtag cgccgcgacg atcgcgtccg
     2761 gatcaaggcc cagctcggac agggtgcgcg ccaccgccag cggatggctg acgtacggct
     2821 cgccgctggc ccgtttctgg gcgccgtgcg cttccgtggc gaattgccag gcggccctca
     2881 cgcgctccag gtccgcggcg ggatagtggg cggccagctc ctcttcgagc ttgcgctccg
     2941 aggcgttgag cgcgcggcgg ctgaagccga acgacagggc gccggtgatg cgctgacgcg
     3001 gccccccgcc tgcggcgcgc tcctgcgggc ggctcagggg ctcgatcgac atcgagcatc
     3061 cagtgtacct tgatgcccct gcgcttccgg ccggccaacg gtgcggcccg tcgccgcgca
     3121 gcccgctgga gtcgatgagc ttgtcctcgt tgctgcgctc aatctcgttc gcgctggcct
     3181 tgctgctggc cgcatgcacg agcgcgccga cacccagcgc gaccggctca cccaccccga
     3241 ctttcgcgcc cagcctcagc gcatcagcta ccgcgagccc gctcgcaacc ccgatcacca
     3301 cgcccggtgc cacgccgacc gccagccctg gtgccagcgc aactccaccg gcgatcccga
     3361 ccccgacccc gaccgccacg ccgccaggca ctctggccac ccggctcgac gcgcagctcc
     3421 gccaggtgct cgatcagcag cgcacaacgc tcgacatccc cggcatttcc gctgcgatca
     3481 ccttccccga cggctcaacc tggacgaccg cgctcgggat ggccgaggtc ttgcccgccc
     3541 gggcggcgac gacatccacc ccgttcgtgg tcggctcggt cagcaagacc ttcgtggcag
     3601 cgaccatcct gcagctcgtc gacgagggcg ctctggcgct cgacgacccg ctatccagct
     3661 ggctgcccga ctatcccctg gctgaccaga tcacgctgcg catgctcctc ggccatacca
     3721 gtggcgtgtt caacctttat gagtcgcctg actacatgcc actggtcgta gaccgtccgc
     3781 atcgcacctg gacgccgcag atggtgctcg acaggctggg cggcgcgccc tactgcgaac
     3841 cgggcacgtg ctaccactac agcaacaccg ggttcatcct gctcgggctg gtcatcgagg
     3901 cggaaaccgg ccagccgctc ggcaccgtct ggcgcgagcg cttcttcacc ccacttggca
     3961 tgagcgacac ctacttccag ggcgacggcc caccgcccgc cacctcggcg ctgggttaca
     4021 ggaagaccaa gaccggcaac gtcccgatca acgacggcag ctcgtaccgc ccgacggagt
     4081 cggagggcac ggttatctgg gccgccggcg gcatcgtcgc gagtgccggc gacatcgctc
     4141 gctggggacg ggcgctatac ggcggtgatg tcctctctga cgcctcgctg gccacgatga
     4201 ccaccttcac cttccacccc gagaagcagg gcggctacta cggcatgggc acccgcatgc
     4261 gcaagttcgg ggccttca
//
LOCUS       DATBRX010000215        12028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_757642, whole genome shotgun sequence.
ACCESSION   DATBRX010000215 DATBRX010000000
VERSION     DATBRX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12028
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757642"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VIF08_08215"
     CDS             <1..1040
                     /locus_tag="VIF08_08215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8136006.1"
                     /translation="EWSLLPVGTRARIGEQLELEVSSSTTPCKTQVANFSDGNFNRIR
                     IELHPSDSRMYARVIQEGEVKAGDPITLLPDVNPQAAIEEMLRRLHRAETKSAVAAWR
                     AAAASGFAVDILEDGEIALASSTDLPGPAFNSGLGLVRLPNMLSMATDFFDAHNTTGY
                     LWCGQAPWPAAKPFLEGDVVGAAVTDSAVVAALAAPMPEGVTIRRIEPGEVNAYNAVR
                     SGERAGGGVTDGAPNPWAQVYEHLAKTHARQLFVAEIDGVAVGNASLHVSAGTGWLRG
                     ALTAPAERGRGIQQALIAHRIRAAIDAGCDLVGADAEPGGVSVRNLKRMGMRRLGRQN
                     NYEYVPRGMRA"
     gene            1037..1849
                     /locus_tag="VIF08_08220"
     CDS             1037..1849
                     /locus_tag="VIF08_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8136007.1"
                     /translation="MSDQLRLDRTGPGAVIARVTLTRPEVHNAFNADLIGELRATFRR
                     LSDEPPDQLRGVVLAGEGPSFCAGADVTWMRASLSLSKEQNEQDAMVMAEMFDAIDRC
                     PAPVVARVQGAALGGGMGLCAVSDLVIAEAGAKFGFTETRLGILPAVIAPFVIAKIGE
                     THARALFPGGRRFDAGRALRIGLVHEVVEGADALDAAVESAVADLLAAGPTAARAAKA
                     VIREVRGLPHESTRWHTARRIAGQRTSAEGQEGLKAFLEKREPGWREGNSGT"
     gene            1988..5158
                     /locus_tag="VIF08_08225"
     CDS             1988..5158
                     /locus_tag="VIF08_08225"
                     /inference="COORDINATES: protein motif:HMM:NF017369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136008.1"
                     /translation="MASQLRLVRGWLPRSLAAAAALLVVWPLGLVASADQTLTGIVGI
                     DHFDDFTAGISREVPVLEVGLDRLELQNASIDELTPGTRVSVHGRRMGDTIFVERDGI
                     SPVGPDTAAAAQTSATATMDKRVAVLMVNFVDPTPSPTPVPTDSPPPTPAPTDTPQPT
                     DSIPPDQTPAPSETPAPSESPAPSDTPLPTPPPTESPSPTPSPSPTPPPQPWTADFLR
                     GVYFTNTRSVADYYSEVSNGQMSVSGDVFGWFTVPADTSACNHTDWGDAARAAATASG
                     VDLAAYSNVVVVFPKQPACWWIGMAQLPGRNSYINGSAGWTGATWLFTPTHELGHNFG
                     MNHASTISCNERGVRVAFSDNCTLDEYGDPYDTMGHDGQRHFNTWHRWQMGALTTADV
                     QTVTATGVYNVAPAEAAGGVPRVLRVARPSGNYFYLEYRQPYGRYDNFSSTAAVVNGV
                     SIRLAAGVGNQSFSKLIDTNPQTATYSDSALAVGRGFADKGSNIFIVTQALSPSGATV
                     LVQVGPDLTLPTAPTNLQTTVGATGAVALSWSAATDDLFVSTYRVSRDGTPLGSAYGT
                     TYTDAAPPQAASHAYSVSAVDGAGNVGPQVSGAIVFVPDTTRPGGVSSLSATAVGPHS
                     VVLAWAAASDNVGVTGYALSRGGQSLATVTGTSFTDDLAPDGYGLSYQVRALDAAGNV
                     GPAATAGISLTDVTAPVLNGDLQLSVEVGGAINLSWPAATDNVGIVGYALTRGGTPLT
                     TVTGTTHVDAGLAQAMDYGYQVVALDAAGNPSSPALSGTIYLPDSTAPSPPGALIVTQ
                     SGPRAVDLHWAAATDNVGVDHYAISVDGVATTTTSAIELVALAMSDGVAHHFELKAVD
                     AAGNLGQPVSADLTLPDVTAPSPVGTFGARASGATSVALSWTGATDNVAVNAYQLTRD
                     GLPLVQLDASARGFTDTGLASDRQHTYGLVAIDAAGNTSTAATAIVRLSSADVAPPTV
                     PQNLQGVALGSRRVSLTWSASSDDRPGTIRYRVFRGSRRIATVTTAGYVDRPASVGWY
                     RYRVKAVDAAGNVSAFSVWVEVRATR"
     gene            5197..7215
                     /locus_tag="VIF08_08230"
     CDS             5197..7215
                     /locus_tag="VIF08_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8136009.1"
                     /translation="MRDAVGPGEAARRLGVSTRTVQRWLREGKLPQVKVGSHVKVLAS
                     ALPPEVGLAGAAQSTRRIGRLLVANRGELVVRIARTCRQLGIRSLGLVVEDQARAWWT
                     RSVDELVPLATSYLDGAAVIAAARAAGADAIHPGYGFLAENADFAEAVNAAGMIWVGP
                     PPAAMRALGDKAAARRLAASLKVPTLPGYDGDDQSDAKLAREAKRIGFPILVKPSAGG
                     GGKGMHVVRAAREMAETLARARREAKASFGDERLILERYLERPRHVEVQLLADEHGHA
                     VHLGERECSLQRRHQKVIEESPSPAVNARLRKKLGEAALTLAREAGYTGAGTAEFLLA
                     PDGAFYFLELNARLQVEHPVSEMVTGRDLVADQLSIAEGRPLGFSQADIRLDGHAVEA
                     RLYAEDPQHEFLPATGEVLFVQWPDMQGVRIDAGVGKDDVVGTRYDPLLAKIIVKAET
                     RKLAYFWLTLALARSSVVGVTNNRGFLIWLSVQPDVLRGQTFTPLIDDQWRPSGELPQ
                     EAWSGAVHALAHHLGEAAGSKVGFRLNAPPRLRLQIEGEVRSVDVPLSAEDLWWAPAG
                     TDSVMLDLDGQAIEARLAPAPTVEAAVRLASHHGASAELIVAPMPGNVLGVRVKEGDE
                     VEAGQVLVVLEAMKMENNVSSPVAGTVARVLVAPGQQVQRAETLVELA"
     gene            complement(7212..7622)
                     /locus_tag="VIF08_08235"
     CDS             complement(7212..7622)
                     /locus_tag="VIF08_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY8136010.1"
                     /translation="MPSGATDRLVLDTSAYARLRAGHTRVLEIVAASAVILLPTIVLG
                     ELEAGFAMGSRAAENRRYLAEFLDAPFVQALDITEGIARRYGETFALLRESGAAMSTN
                     DVWIAATTLDCGGTLATFDRDFDRIAGLPLVLLT"
     gene            complement(7603..7848)
                     /locus_tag="VIF08_08240"
     CDS             complement(7603..7848)
                     /locus_tag="VIF08_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136011.1"
                     /translation="MARQLTVRGVPDEVASRLEKISRQRGQSLNATINALLAQAAGED
                     ARRRWLERFVTWTAADLAEFDAALAAQRTIDDALWRD"
     gene            7924..8868
                     /locus_tag="VIF08_08245"
     CDS             7924..8868
                     /locus_tag="VIF08_08245"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY8136012.1"
                     /translation="MSYPSHVRIYEVGPRDGLQNEATPISTADKLRYISLLRDAGLRE
                     IEATSFVRPNAIPQLADADELMLALASDQDAAGVRCPVLVPNERGLDRAVATGANALA
                     VFTAASDAFTQRNIGMTIDESLAAFAPVLRRAADMGWWRRAYVSTAFGCPFTGRVEVA
                     KVVEVALRLDELGVDEICIGDTIGVGVPGQVHDITGAVAAAGIPTDRIAYHFHDTRGT
                     ALANVVAGLEDGISCFDASTGGTGGCPYAPGAAGNLATEDLVYLLDGMGIAHGVDLGK
                     VIAAARFIATALGKPLASKVGQAGGWDSATGQRLLPGA"
     gene            9045..9560
                     /locus_tag="VIF08_08250"
     CDS             9045..9560
                     /locus_tag="VIF08_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8136013.1"
                     /translation="MDSVVLLLNADYEPLNVCNLRRAFRLVFGEKAEVIQYDHTEVRT
                     ATQIFHAPSVIRLQHRIKRPRPRVRLSRREVFLRDKHTCQYCGAQTHDLTLDHVIPRH
                     RGGMHTWDNLVTACKHCNHRKGGKLVDEARMRLQRPPFEPRCDVYTLFTPYLSDPRNE
                     AWRDYLFLGTN"
     gene            9598..10902
                     /locus_tag="VIF08_08255"
     CDS             9598..10902
                     /locus_tag="VIF08_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8136014.1"
                     /translation="MLQTLWDAGHAAYLVGGGVRDALLGKAVTDWDVATDALPERLLE
                     LFPGGGYDNRFGTVRVPSADLELEVTTFRRDHQYADHRRPDSVTFTESLDEDLARRDF
                     TVNAIAWSRDGLVDPTTGLADLQSGLLRAVGDPRVRFDEDALRLLRAARLAAQLDFEI
                     EPSTLAAMSATAAGMQYVSAERIGEELRKILRAAQPSRALAILADTGVLKFALPELDA
                     QRGIAQDKAPGMDLWAHCLAATDAAARLAPGNPRLRLAALVHDIGKPGTLADGHFIGH
                     DTEGARLAEQMLGRLAFPRRDVDAVADLVRYHMFSYEPRWSGAAVRRFIRRVGRNLVD
                     ELLDLRAADNLGSGLPANAGHLDELRSRVAAELAAGAPLTLRELDVRGDDLTAALDLA
                     PGPVVGELLDHLLQWVLDDPARNDRDALIAEARAQMDSGSGS"
     gene            10899..11306
                     /locus_tag="VIF08_08260"
     CDS             10899..11306
                     /locus_tag="VIF08_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8136015.1"
                     /translation="MIEALLQAERLLVHGMVDQAEGIYQRAIEQDPQNAIAVVGLARV
                     ALERGQERVAYERAREALAIDPQNVAAQRLEARLAEVFAEQDRPTDPPPAEPTSSELP
                     NPFEPSERAFLTRNPSMADHAAADERRRRILEE"
     gene            11320..>12028
                     /gene="galE"
                     /locus_tag="VIF08_08265"
     CDS             11320..>12028
                     /gene="galE"
                     /locus_tag="VIF08_08265"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY8136016.1"
                     /translation="MRVLVTGGAGYVGSTTAQRLIEAGHEVVAYDSLELGHRAAVPEG
                     AVLVSGYIGDQGLVEATLREHSIDAVLHCAGYSLVGESVQQPQRYFENNVARGIAFLD
                     SLLAAGVRRVVFSSSAAVYGVPDRIPIEETDTLAPVNPYGATKLALENALTSYATAYE
                     WRVVSVRYFNAAGATDKLGEDHRPETHLIPNILAAVERGDTVRIFGSDYPTPDGTCVR
                     DYIHVDDLAAAHIAALEL"
BASE COUNT         1952 a         4151 c         4084 g         1841 t
ORIGIN      
        1 tcgaatggtc gctgctgccg gtgggcacga gggcgcgcat tggcgagcag ctcgagctgg
       61 aggtctcgtc ctcgacaacg ccctgcaaga cacaggtggc caacttcagc gacggcaact
      121 tcaaccgcat ccgcatcgag ctgcacccgt ctgacagtcg gatgtacgcc cgtgtgatcc
      181 aagaaggtga ggtcaaggcg ggcgacccga taacgctgct gcccgacgtc aaccctcagg
      241 cggcaatcga ggagatgctg cgccgtctcc accgggcgga gacgaagtcc gctgttgctg
      301 cctggcgcgc tgccgcagcg tcaggtttcg cggtcgacat cctcgaggat ggcgagattg
      361 ccctggcgtc atcgaccgac ctgcccggtc cggccttcaa ctcgggcctg ggcctcgttc
      421 gcttgcccaa catgctgtcg atggcgaccg acttctttga cgcgcacaac acgaccggct
      481 acctgtggtg cggccaggcg ccctggcccg cagccaagcc gtttctggag ggcgatgtag
      541 tcggcgctgc cgtaacggat tccgcggttg ttgccgcgct tgccgccccg atgccggaag
      601 gcgtgaccat ccggcgcatc gaaccgggcg aagtcaatgc ctacaacgcg gtccgatcag
      661 gcgagcgcgc aggcggcggg gtcaccgatg gcgcgccgaa cccctgggcg caggtatacg
      721 aacatctcgc caagacccac gcgcgtcagc tgttcgtggc tgagatagac ggcgtcgcgg
      781 tgggcaatgc gtcgctccac gtgagtgccg gcaccggctg gctacgcggc gcattgaccg
      841 cacccgcaga gcgcggccgc ggcatccagc aggcgttgat cgcgcaccgc attcgcgcgg
      901 cgattgacgc tggctgcgac ctcgtcggcg cggacgcgga accgggcgga gtgtcagtgc
      961 ggaacctgaa gcgcatgggc atgcgccgtt tgggccgcca gaacaactac gaatacgtgc
     1021 cgcgcggaat gcgcgcatga gcgaccagct gcgcctggat cgcacggggc cgggggcagt
     1081 catcgcgcgg gtcacgctga cgcggccgga ggtccacaac gcgttcaacg ccgacctgat
     1141 cggcgagctg cgggccactt tccggcgtct ctccgacgag ccgccggacc agctgcgcgg
     1201 cgtcgtcctc gcaggtgaag gtccgtcgtt ctgcgccggc gcggacgtga cgtggatgcg
     1261 tgcgtcgctg agcctgagca aggagcagaa cgagcaggac gcgatggtca tggccgagat
     1321 gttcgacgcg atcgatcgct gcccggcgcc ggtcgtggcg cgcgtccagg gcgcggcgct
     1381 gggcggcggg atgggcctgt gcgcggtgtc cgacctcgtc atcgcggaag ccggcgcgaa
     1441 gttcggcttc acggagaccc gcctgggcat cctacccgcg gtcatcgcac ccttcgtcat
     1501 cgccaagatc ggcgagacgc acgcccgggc gctcttcccc ggcggccgtc gctttgacgc
     1561 gggccgggcg ctgcgcattg gcctggtcca cgaggttgtc gaaggcgcgg acgcattgga
     1621 cgcggcggtc gaatctgcgg tcgccgacct tctggccgcg gggccgaccg cggcgcgcgc
     1681 cgcgaaggcg gtgatccgcg aggtgcgcgg tctgccccac gagtcgacgc gctggcacac
     1741 cgcgcgccga attgccggac aacgaaccag cgcggagggc caggagggcc tcaaggcctt
     1801 cctcgagaag cgcgaaccgg gctggcgcga gggcaacagc ggcacctgat tcggcggttt
     1861 cctgctccgt ctgaacactt cgggtcacgg acgcgcgaga tcacgcagcc ggaggatggc
     1921 tgggggtcta gcctgagctc aggctcgggg ccgtaaccgg gccagcttca gggggaagcg
     1981 tcggggaatg gcgtcacaac tccgcctagt tcgcggctgg ctgccgcgct cacttgctgc
     2041 cgccgctgcg ctgctcgttg tctggccact cggcctggtt gcgagcgccg atcaaacgct
     2101 caccggtatt gtcgggatcg accatttcga cgacttcacc gccgggatct cgcgcgaggt
     2161 gcccgtcctg gaggtcgggc tagatcggct ggagctgcag aacgcatcga ttgacgagct
     2221 gaccccggga acgcgcgtca gcgtccatgg tcggcggatg ggcgacacca tcttcgtcga
     2281 gcgcgacggc atcagcccgg ttgggccaga caccgctgca gccgcgcaga ccagcgccac
     2341 ggccacaatg gacaagcgcg tggcggtgct gatggtcaac ttcgtcgacc caacgccgtc
     2401 gcccaccccg gttccgaccg attcgccgcc gccaacccct gcccccacgg acaccccgca
     2461 gccgacagat tcgatcccgc cggaccaaac gcctgcaccc tccgaaacgc cagcgccaag
     2521 tgaatcgccc gcgccatcgg atacgccgtt gcccacgcca ccgccgactg aatcgccatc
     2581 gccgacgcct tcgccgagcc caacaccgcc gccgcaacca tggacggccg atttcctgcg
     2641 cggcgtgtac ttcaccaaca cccgatcggt cgccgactac tacagcgaag tctcgaacgg
     2701 ccagatgtcc gtcagcggcg acgtgttcgg ctggttcacg gtccccgctg acacaagcgc
     2761 ctgcaaccac accgactggg gcgacgcagc ccgggccgcc gcgaccgcgt cgggtgtcga
     2821 tctcgcggcc tacagcaacg tggtagtggt ctttccaaag caaccggctt gctggtggat
     2881 cggcatggcg cagctgccgg gtcggaacag ctacatcaac ggctcggctg gctggaccgg
     2941 tgcgacctgg ctgttcacgc ccactcatga gctgggccac aacttcggca tgaaccatgc
     3001 gtcgaccatc tcgtgcaacg agaggggggt gcgggtcgcg ttctccgaca actgcacgct
     3061 cgacgagtac ggcgatccgt acgacacgat gggccacgac ggccagcgcc acttcaacac
     3121 gtggcatcgc tggcagatgg gcgcgctgac cacggccgac gttcagaccg tcaccgcaac
     3181 gggcgtttac aacgtcgcgc ccgcggaagc ggccggcgga gtgccgcgcg tcctgcgtgt
     3241 cgcgcgaccg tcgggcaact acttctacct cgagtaccgc cagccctatg ggcggtacga
     3301 caacttctcg agcactgcgg cggtggtcaa cggtgtgtcc atccgcctcg cggcaggggt
     3361 cggcaatcag agcttctcca agttgatcga caccaatccc cagaccgcga cctactccga
     3421 ctccgccctg gcggtcggcc gcggcttcgc ggacaagggc agcaacatct tcatcgtcac
     3481 gcaggcgctc tcgccatctg gcgcgacggt ccttgtccag gtcggtccag atttgacgct
     3541 gccgactgcg cccaccaatc tgcagacgac ggtcggcgcg accggcgcag tcgcgctcag
     3601 ctggagtgcc gcgaccgacg acctgttcgt gtcgacgtac cgggtcagcc gcgacggaac
     3661 tccgttgggc agcgcgtatg gcacgacgta caccgatgcc gcgccgccac aggcagccag
     3721 ccacgcgtac tcggtcagcg ccgtcgacgg cgccggtaac gtgggtccgc aggtgagcgg
     3781 cgccatcgtg ttcgtgcccg acacgacgcg gccgggcggc gtcagctcgc tgtccgctac
     3841 ggccgttggt ccgcactccg tggtcctggc gtgggccgca gcgtcggaca acgtcggcgt
     3901 tacgggatac gcgctcagcc gcggcgggca atccctggcg acggtgacgg gcacgagctt
     3961 caccgatgat ctggccccgg acggttacgg cctttcctac caggtccgag cgctcgacgc
     4021 ggcaggcaac gttgggccgg ccgcgacagc cgggatcagc ctgaccgatg tcactgcccc
     4081 ggtgctgaac ggtgacctgc agctgagtgt ggaggtgggc ggcgcgatta acctgtcgtg
     4141 gccggccgcc acggacaacg tcggcatcgt gggttacgcg ctgacccggg gtggcacgcc
     4201 actgacaaca gtcaccggga cgacacatgt cgatgccggc ctggcacagg ccatggacta
     4261 cggctaccag gtcgtcgcgc tggacgcggc cggcaacccg agctctccgg cgctgagcgg
     4321 caccatctat ctacccgaca gcacagcacc gtcgccgccg ggcgctctga tcgttaccca
     4381 gagtgggccg cgcgcggttg atctccactg ggcagcggca actgacaacg taggcgttga
     4441 ccactacgcg atctcggtcg atggcgtggc caccacgacc acgagcgcga tcgaactcgt
     4501 agccctcgcc atgtccgatg gagtcgccca ccacttcgag ctcaaggccg tcgatgcggc
     4561 gggcaacctg gggcagccgg taagcgcaga tctgacgctg cccgatgtga ctgcgccatc
     4621 gcctgtcggc accttcggcg cccgcgccag cggagccaca tcggtcgcgt tgagctggac
     4681 cggtgccacg gacaacgtgg ccgtcaacgc ctatcagctg acgcgtgacg gactgccgtt
     4741 agtgcagctc gacgcgtccg cgcgcggttt taccgatacc gggcttgcga gcgatcggca
     4801 gcacacctat ggcctcgtcg cgatcgacgc ggcgggcaac acgagcaccg cggcgaccgc
     4861 gatcgtgcgc ctcagctcgg ctgacgtcgc cccacccacc gtgccgcaga acctgcaagg
     4921 cgtggcgctg ggcagcaggc gagtgagcct gacctggagc gcgagcagcg atgatcgccc
     4981 gggcacgatc cggtatcggg tcttccgggg cagccgccgc atcgccacgg tcacgaccgc
     5041 cggctacgtc gaccggccgg cgtccgtggg ttggtaccgc tacagggtca aggccgtcga
     5101 cgcggcaggc aacgtcagcg cgttttcggt ctgggtggaa gttcgcgcga ctcgctgatc
     5161 gccacacagc gcgacaaacg tgacatcctg acgcgcgtga gggacgcggt tggtccaggc
     5221 gaagcggcgc gcaggctggg agtctcgacc aggaccgtcc agcgctggct gcgcgaaggc
     5281 aagctgccgc aggtcaaggt cgggtcgcac gtcaaggttc tagccagcgc cttgccgcct
     5341 gaagtgggcc tggcgggcgc ggcgcagtca acccgaagga tcggtcggct gctggtcgcc
     5401 aatcggggcg agctggtcgt ccgcattgcc cggacgtgtc ggcagctggg tattcgctcg
     5461 ctgggcctcg tcgtcgaaga ccaggcacgt gcctggtgga cgcggtcggt tgacgagctc
     5521 gtgccgctcg cgacctcgta cctggacggg gccgcggtga tagccgctgc gcgtgccgcc
     5581 ggcgccgacg ctatccatcc cggctacggc ttcctggcag agaacgcaga ctttgccgag
     5641 gcggtcaatg ccgccggcat gatctgggtc gggccgccac ccgcagccat gcgtgctctt
     5701 ggcgacaagg ccgccgcgcg gcggctcgcg gcgagtctca aggtgcccac gctgcccggc
     5761 tacgacgggg acgaccaatc ggacgcgaag ctggcgcgcg aggcaaagcg catcggcttt
     5821 cccatcctgg tcaagcccag cgccggcggt ggcggcaagg gcatgcatgt cgtccgggcc
     5881 gcccgagaga tggccgaaac cctggcccgg gcacggcgcg aggccaaggc gtcgttcggc
     5941 gacgagcggc tgatcctgga gcgctacctg gagcggccgc gccacgtgga ggtgcagctg
     6001 ctcgctgatg agcacggcca cgccgttcac ctaggtgagc gcgagtgctc gctccagcgc
     6061 cgccaccaga aagtgatcga ggaatcaccg tcacccgcgg tgaacgccag gctgcgcaag
     6121 aagctgggcg aggccgcgct gacgctggcg cgcgaggcgg gctacacggg cgctggcacg
     6181 gcggagttcc tgctcgcacc agatggcgcc ttctacttcc tcgagctcaa cgcccgactg
     6241 caggtcgagc atccggtcag cgagatggtc actgggcgcg acctcgtcgc cgaccagctg
     6301 agcatcgcgg agggccgacc actcggcttc agccaggccg acatcaggct cgacggccac
     6361 gcggtcgagg cgcgcctcta cgccgaggac ccgcagcacg aattcctgcc ggccaccggc
     6421 gaagtgttgt tcgtgcagtg gccagacatg cagggcgtgc gcatcgacgc gggagtcggc
     6481 aaggacgacg tcgttggcac gcgctatgac ccgctgctgg ccaagatcat cgtcaaggca
     6541 gagactcgca agttggccta cttctggctg accctcgcgc ttgcccggtc atcggtcgta
     6601 ggcgtcacca acaaccgcgg cttcctaatc tggctttctg tccagcccga cgtgctgcgc
     6661 ggccagacct tcacgccgct catcgacgat cagtggcggc cgagcggcga gctgccccag
     6721 gaggcatggt ccggggcagt gcacgcgctg gcccatcacc tgggcgaggc cgctgggtcg
     6781 aaggtcggct ttcgccttaa cgcaccaccg cggctgcgcc tccagatcga aggcgaagtg
     6841 aggtcggtcg atgtcccgct gagtgcagag gacctgtggt gggcgccggc cggtaccgat
     6901 tcggtgatgc tcgatctgga cggccaggcg atcgaggcgc gacttgcgcc agcgcccacc
     6961 gtcgaggcgg ccgtccggct cgcgagccac catggtgcct cagcggagct catcgtcgcg
     7021 cccatgccgg gcaacgtgct cggggtgcgc gtcaaggagg gcgacgaggt cgaggccggc
     7081 caggtgctcg tcgtcctcga ggcgatgaag atggagaaca acgtttcatc gccggtggcc
     7141 ggaacagtag cgcgtgtgct ggtggcgcca ggtcaacagg tgcagcgcgc cgaaactctc
     7201 gtagagctgg cctaggtcaa gaggaccagc ggtaggccgg cgatccggtc gaagtcgcga
     7261 tcgaaggtgg ccaaggttcc gccgcaatcg agggtcgtgg ccgcgatcca gacatcattc
     7321 gtcgacatgg ccgcgcccga ttcgcgaagc aacgcgaacg tctctccgta acgtcgggca
     7381 atgccttcgg tgatatcgag agcctggacg aagggggcat cgagaaactc agccaggtac
     7441 ctgcgattct ccgctgcgcg gctgcccatg gcgaaaccgg cctccagttc cccaaggacg
     7501 atggtgggaa gcaggatcac ggccgatgca gccacgatct ccaggacccg cgtgtgccct
     7561 gcgcgaaggc gcgcataggc ggaggtatct aggactagac gatcagtcgc gccagagggc
     7621 atcgtcgatc gtccgctgcg cggccagagc ggcgtcgaac tcagcgagat cggcggcagt
     7681 ccacgtcacg aaacgctcga gccaccttcg acgagcatcc tcgccagcgg cttgcgccaa
     7741 tagcgcgttg atcgtcgcgt tcagactctg accgcgttgc ctgctgatct tctcgagccg
     7801 gctcgcgacc tcatctggga ctccacgaac tgtcagttgc cgcgccatat gtaggcatgc
     7861 tatcaccaat gtgcctgcat gtaggcactt tcaccgccac gaggcgttat ctagactcgt
     7921 gtcgtgagtt acccatcgca cgttcgcatc tacgaggtcg gtccgcgcga cggacttcag
     7981 aacgaggcga cgcccatctc gaccgccgac aagctgcgct acatctccct cctgcgcgac
     8041 gcgggcctgc gcgagatcga agcgacgtcc ttcgtccggc ccaacgcgat tccccaactg
     8101 gccgacgcgg acgagctgat gctggcgctg gcatctgacc aagatgcggc gggcgttcgg
     8161 tgccccgtgt tggtgccgaa tgaacgcggg ctcgaccggg cggtcgccac aggtgccaac
     8221 gcgctggcgg tcttcaccgc ggcgagcgac gccttcacgc agcgcaacat cgggatgacg
     8281 atcgacgaat cgctggctgc gttcgcgccc gttctgcgcc gcgcggcgga catgggctgg
     8341 tggcgccgag cgtacgtgtc gaccgcattc ggctgcccgt tcaccggccg cgtcgaggtc
     8401 gccaaggtcg tcgaggtcgc cctgcggctg gacgagctgg gcgtcgatga gatctgcatc
     8461 ggcgacacga tcggcgtggg cgtgcccggc caggtccacg acatcacggg cgcggtcgcc
     8521 gccgcaggca tcccgacgga ccgcatcgcg taccacttcc acgacacgcg cggcacggca
     8581 ctcgccaatg tcgtggccgg gctggaagac ggcatcagct gtttcgacgc ctcgaccggc
     8641 ggcaccggcg gttgtccgta cgcgcctggc gccgccggca acctcgcgac cgaggatctg
     8701 gtctacctgt tggacggtat gggcatcgcc cacggcgtcg acctgggcaa ggtcatcgcg
     8761 gccgcgcgct tcatcgccac ggctctcggc aagccgctcg ccagcaaggt cggccaggcc
     8821 ggcggctggg actcagctac cggtcagcga ctcctgcccg gcgcctgacc acacctgcgc
     8881 cagcaccagg aggcgcggct ggcattcgcc caggtcgcgg atggagcacg tgggagttgg
     8941 cgcgtcgggg tccatttcgg ccattgtgcg ccaccggtat ccacgctcac ttttgctata
     9001 ttccgagagt cgcccggccg agtcccggct ggcttcaggg agaaatggac agcgtcgtac
     9061 tccttctcaa cgccgactac gagccgctca acgtctgcaa cctgaggcgc gcattccgcc
     9121 tcgtcttcgg cgagaaggca gaagtaatcc agtacgacca caccgaggtc cggaccgcga
     9181 cgcagatctt ccacgccccc tcggtcatcc gcctgcagca ccgaatcaag cgcccgcgtc
     9241 cgcgcgtccg cctctcgaga cgcgaggtgt tcctgcgcga caagcacacg tgccaatact
     9301 gcggcgcgca gacgcacgac ctgacgctgg accacgtgat cccgcgccac cgcggcggga
     9361 tgcacacgtg ggacaacctc gtcaccgcgt gcaagcactg caaccaccgc aagggcggca
     9421 agctggtcga cgaggcgcgg atgcgcctgc agcgaccgcc cttcgagccg cgctgcgacg
     9481 tctacacgct gttcacgcct tacctgagtg acccgcgcaa cgaggcctgg cgcgactacc
     9541 tgttcctcgg aacgaactga gttggagccg gtcaacgcac cggccccggt gatggcggtg
     9601 ctccagaccc tgtgggatgc gggccacgcc gcctatctcg tgggcggggg cgtgcgcgac
     9661 gccctgctgg gcaaggcagt caccgactgg gacgttgcca ccgacgctct gcccgaacgc
     9721 ctgctggagc tctttccggg cggcggttac gacaaccgct tcggaacggt cagggtgccg
     9781 tcggccgatc tcgagctgga ggtcacgacc ttccgacgcg accaccagta cgccgaccat
     9841 cgccggcctg actcggtgac gttcaccgaa tcgctcgacg aggacctggc gcggcgtgac
     9901 ttcacggtga acgcaatcgc ctggtcgcgc gacgggttgg tcgatccgac gacaggcttg
     9961 gccgaccttc agtcggggct gctgcgcgcg gttggtgacc cgcgcgtgcg cttcgatgag
    10021 gacgcgctgc ggctgctgcg ggccgcgcgg ctggcggcgc agctcgactt cgagatcgag
    10081 ccgtccactc tggccgcgat gagtgccacg gctgccggca tgcagtacgt gtccgcggaa
    10141 cggatcggcg aggagctgcg caagatcctg cgcgccgctc agccctcgcg cgccctcgcg
    10201 atcctcgcgg ataccggcgt gctgaagttc gcgctgccgg agctggacgc gcagcgcggc
    10261 atcgcccagg acaaggcgcc gggcatggac ctgtgggcgc actgcctggc cgcgaccgac
    10321 gctgccgcgc ggctggctcc gggcaatccc cggctgcgtc tggcggcgct ggtccacgac
    10381 attggcaagc cgggcacgct ggccgacggc cacttcatcg gccacgacac ggagggcgcg
    10441 cggctggcgg aacagatgct ggggcgcctc gccttcccac ggcgcgacgt cgacgccgtc
    10501 gcggatctcg tgcgctacca catgttcagc tacgagccgc gctggagcgg cgcggccgtg
    10561 cggcgcttca tccgccgcgt ggggcgcaat ctcgtcgacg agctgctcga cctgcgcgcg
    10621 gccgacaacc tgggcagcgg cctgccggcg aacgcgggcc acctcgacga gctgcgctcg
    10681 cgtgtcgccg ccgagctggc ggcaggcgcg ccgttgaccc tgcgcgagct ggacgttcgc
    10741 ggcgacgacc tgacagccgc tctggacctg gcgcctggtc ccgtcgtagg cgagctgctc
    10801 gaccacctgc tccaatgggt cctagatgac cccgcgcgca acgaccgcga cgcattgatt
    10861 gccgaggccc gcgcccaaat ggatagtggg agcggctcgt gatcgaagcg ctcctccagg
    10921 ctgagcggct gctcgtccat ggcatggtcg accaggccga gggcatctat cagcgcgcca
    10981 tcgaacagga tccgcagaac gcgatcgcgg tcgtcggcct ggcgcgcgtc gcgctggagc
    11041 gcggccagga gcgggtcgcg tacgagcgtg cgcgcgaggc gctcgcgatc gatccgcaga
    11101 acgtcgccgc acaacggctg gaggcgcgcc tcgccgaggt gttcgcagaa caggaccgcc
    11161 ccaccgaccc acccccggcg gaaccaacct cgtccgagct gccgaacccc ttcgagccat
    11221 ccgagcgcgc cttcctgacg cgcaacccat ccatggccga ccacgccgcg gccgatgagc
    11281 gccggcggcg gatacttgaa gagtgagagt caacggctga tgagagtcct ggtaaccggc
    11341 ggcgcgggct acgtcggcag caccaccgcg caacgcctca tcgaggccgg ccatgaggtg
    11401 gtcgcctacg actcgctcga gctcggccat cgtgctgctg tgcctgaagg tgcggtcctc
    11461 gtgagcggtt acatcggtga tcaggggctc gtcgaagcca cactccggga gcattccatc
    11521 gacgccgtcc tgcactgcgc cggctactcg ctcgtgggtg aatcggtcca gcagccgcag
    11581 cgctatttcg aaaacaacgt cgcacgcggg atcgcgttcc tggacagcct gctcgcggcc
    11641 ggcgtcaggc gcgttgtgtt ctcatcgagc gcggcggtct acggcgtgcc ggataggatc
    11701 ccgatcgagg agacggacac gctcgcgccc gtcaaccctt acggcgcgac caagcttgcc
    11761 ctcgagaatg cgctgacttc ctacgcgacc gcctatgagt ggcgcgtcgt ctcggtgcgc
    11821 tatttcaacg ccgccggagc gacggacaaa ctgggcgagg atcaccggcc cgagacacat
    11881 ctcatcccga acatcctcgc ggcggtcgag cgcggcgaca cggtcaggat cttcgggagc
    11941 gactatccca cgccagacgg cacgtgcgtc cgtgactaca tccacgtcga tgacctcgcg
    12001 gccgcccaca tcgcggcgct ggagctga
//
LOCUS       DATBRX010000216         9942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2657735, whole genome shotgun sequence.
ACCESSION   DATBRX010000216 DATBRX010000000
VERSION     DATBRX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2657735"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VIF08_08270"
     CDS             complement(<1..455)
                     /locus_tag="VIF08_08270"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8136017.1"
                     /translation="MSQAIGVFSASEMMIVAAARELAGQRVCFVGVGLPNIAVNLAKR
                     TVAPDLELVYEAGVFGARPARLPLSIGDPTIVTHATAVTSMFELFGYYLQGGLIDVGF
                     LGAAQIDRHGDLNSTVIGPYETPKTRLPGSGGACEIAQNAKSIFVIMRQS"
     gene            complement(473..1546)
                     /locus_tag="VIF08_08275"
     CDS             complement(473..1546)
                     /locus_tag="VIF08_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136018.1"
                     /translation="MAGGVAAALLLTVWAPGAMAAPRPLTVDVLLGDSCVSGEARKNA
                     FVKVVVRDPAGNMKMREAVDTGPYGEWGVCGYSGTITAGDKINVLVVETGQTRKLTVP
                     KLTIDANRVSEVVSGKAPAGSMITIAVSSAGASTVGLPEYDVTQSVVTSAGGAYSFDF
                     TTDGIDLIGGATAEATWRSAGGAVQVHRRTFVPGLIVAIGEAQFAGTFKPNAHLGIIV
                     TQGSTQVATGDAVADPFYGGQVFGQFVDADAEPYAIAAGDWINAPGLGSDGTFQVPLI
                     NGTANLSSERVTGTCFANGLYVVAVLGPDYLDYGFTFGTAAANGKFTADLSDQLNIRK
                     NFEVQIGCYTANADLVVGTFITH"
     gene            complement(1762..2874)
                     /locus_tag="VIF08_08280"
     CDS             complement(1762..2874)
                     /locus_tag="VIF08_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136019.1"
                     /translation="MRSKGLFRVALKPAVLAGLLGSVLASSFAGVALAAPPPITFELF
                     IGDSCVYGMAKPDSFLKVIITDKSGSQKGRGAVETGPDGYWEACMQQGVRPLSPGDTI
                     KVTDFETGQKRNIKVPTLTAKVDRGANVISGKAPAGTTVEVEAFDFRFDLWGEQYDVV
                     QHTVAVGGAYSYDFDNDGVDIKGGASLVVRWHNGGDTVTVGRFQIAPYIVLQLGLSDF
                     AGAAGPNTPIAMTLTRDGAIVARGNGVGSYSNSSFYGDFVDADGETYIVRGGEKLNAP
                     ALGAVSGWRIPAIKGTANVAADSVSGKCFANGRFVILAQGISEPGYGFGFGTANADGS
                     FTVSLGDQVDIKKNFRVGILCYSAEGDEVSQEFMTR"
     gene            complement(2900..3820)
                     /locus_tag="VIF08_08285"
     CDS             complement(2900..3820)
                     /locus_tag="VIF08_08285"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8136020.1"
                     /translation="MSGTSKVATMRDAIAELVRDGDSVAIEGFTHLIGFAAGHEIIRQ
                     GRRDLTLCRLTPDLIYDQMVAAGVARKLVFSWLGNPGVGSLHAIRRWAEEIPSKLELE
                     EYSHFGMVCRYTAGAMNLPFFPLRSYENTDLPIANPLIRPIRSPYSDEELYAVPALKP
                     DVTIVHAQRADAAGDTQVWGLLGCQKEAAFAADRVIVVVEELVDESVIRADPNRTILP
                     GLVVDAVVVEPFGAHPSYAQGYYDRDNAFYLEWETISRDGASLARWLDEWVHAVSGRT
                     EYLEKLGRARVEALRPQPAPSGSVDYGDYR"
     gene            complement(3860..5227)
                     /locus_tag="VIF08_08290"
     CDS             complement(3860..5227)
                     /locus_tag="VIF08_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8136021.1"
                     /translation="MTELAERPQLQGTARNVPDIREPLPGPKARKHVAFDHEFTSPSL
                     PRAYPLVPVRGTGSTVEDIDGNLLLDFTAGIAVSSTGYAHPRITAALEKQARDLIHFS
                     ASDFYLPIYAELAAKLAEISPIKGPARAFIGNSGAEAIEAGIKLARFATGRQYLVAFI
                     GGFHGRTYGAVTLTASKSKYHAGFGPMLPGVYHVPFGNAGLDELENRVFKRLIPANEI
                     AAIVVEPIQGEGGYVIPEPDFFARLRKICDDNGILLIADEVQSGAGRTGKMWAIDHWG
                     VQPDIVATAKGIASGMPLGAMIARADLMSWGKGHHGSTFGGNAVSCAVALETIKLLQE
                     GLMDNAAARGEQALAGLRPLMGQHPKLVTDVRGKGLMLGVEFDTGETAEAVQWACFTR
                     GLLVLEAGDSVVRMCPPLVVTAQEIDTCVRLFTDAVAEVAKDPTRAYDESRRWMSIDD
                     GEVDG"
     gene            complement(5452..5766)
                     /locus_tag="VIF08_08295"
     CDS             complement(5452..5766)
                     /locus_tag="VIF08_08295"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY8136022.1"
                     /translation="MVVGDVVLAQSTGRRIIKNGPDAPWRKVAGFLDQAELFMINLEC
                     PISDRGTSWPGEKYTLRARPASADALPAAGVDVANHTAWATSSSPTSVVCRMTRPSWT
                     SP"
     gene            complement(5890..6750)
                     /locus_tag="VIF08_08300"
     CDS             complement(5890..6750)
                     /locus_tag="VIF08_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY8136023.1"
                     /translation="MGQGERSSRLHRSRARAVLALALTLTLFASPARAGDAPPCRTGD
                     ELVAYDGGRWSRVVIDTWWRLSGAYAPNDLVSVRRAHIPGSGKVRGLVIDDLSAMAAD
                     ARAQGIRLAVQSAYRSYGAQASTFREWVRKIGLNRALLSSARAGHSEHQLGTAVDFKS
                     PGGPVPWANYPRWERTPISRWLAANAWRYGFILSYPKSARETTCYLYEPWHYRYVGRD
                     IAREIHDGGQPPRIWMLSHPNPSPSPTPTPTGTPTPTAEPTAEPTAEPTAEPTAESTA
                     EPTSEPSPIQ"
     gene            complement(6792..8375)
                     /locus_tag="VIF08_08305"
     CDS             complement(6792..8375)
                     /locus_tag="VIF08_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8136024.1"
                     /translation="MTDTIERPRLKITYATLRNDNEELHEQFEHGMAKARAQLGKHYP
                     NFVNGEARQGEGEFEDHSPIDDSLIVGYFAKGTRQDAKDAIAAARAAFPGWRDTPWQE
                     RVAIIRRAAEIISSRQMELAALMVLDVGKNRLEALGDVEETADFCRYYSERMEENNGF
                     DHPMDNLGDATVRTRSVLKPHGVWAVVSPFNFPMALAGGPSIAAILTGNTVVCKPSSD
                     APLLAMKLYEILVEAGVPKGVFNIVTGPGETVGAELVESDDVDGIVFTGSFEVGFDLY
                     KHFAKRYPKPVIAEMGGKNPTVVSHSADLDKAADGVMRAAFGFGGQKCSANSRVYVER
                     EVHDAFVEKLVERARQIHVGDPSDRANWLGPIINTKALARYENAVAEAKRDGRVAFGG
                     ERLTDGDLSHGYYVAPTVVTGLPATHRLFRDELFVPIVAVAPVDSIDEGIKLANESEY
                     ALTAGFYSEDEAEQQKFLDTIHGGVVYVNRRAGATTGAWPGVQPFGGWKGSGSTGKAG
                     GGLYYIQQFMREQSQTVVD"
     gene            8551..9879
                     /locus_tag="VIF08_08310"
     CDS             8551..9879
                     /locus_tag="VIF08_08310"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY8136025.1"
                     /translation="MTTLTPAERPDAGLGPDTAELRIGERTYSFPIVRGTEGYPAINV
                     TTLLRDAGMITLDYGFGSTAATRSGITYLDGDAGVLRYRGYPIEQLAEGSTFLETAYL
                     LMYGELPSGEQLAAWTDLVTRHTLLHEEFKRFFEGFPMDAHPMGILAAAVAALSTFYQ
                     QSMDPRDADSVDISTVRLIAKMPTIAALAYKHSVGQPFIYPDNSLDYESNFLRMMFAI
                     PAEPYRIDPDAARALRVLLILHADHEQNCSTATVRLAGSSHANLYASISAGISALWGP
                     LHGGANQEVLEMLQQIEADGGNVAKWVDRAKDKNDSFRLMGFGHRVYKNYDPRARIIK
                     AAADTILAKLGAEDRLLEIAKELEAVALTDPYFIDHKLYPNVDFYSGLIYRALGFPTE
                     MFTVLFAIGRLPGWIAQWREMMTDPDSRIGRPRQVYTGARERKYLPLDQR"
BASE COUNT         1599 a         3323 c         3335 g         1685 t
ORIGIN      
        1 gactggcgca tgatcacgaa gatcgacttc gcgttctggg cgatctcaca cgcgcccccg
       61 gagccgggca gccgcgtctt gggcgtctcg tacggcccga tcaccgttga gttgaggtcg
      121 ccatggcgat cgatctgcgc cgcgcccagg aacccgacat cgatcagccc gccctggagg
      181 tagtagccga agagctcgaa catgctcgtc accgcggtcg cgtgggtgac gatggtcggg
      241 tcgccgattg acagcggcag ccgagccgga cgggcaccga acacgccggc ctcgtacacc
      301 agctccagat cgggcgccac ggtgcgcttc gcgaggttga ctgcgatgtt cggcaggcca
      361 acgccgacga agcacacccg ctgtccggcc agctcgcgcg cggcggcgac gatcatcatc
      421 tccgaggcgg agaagacccc aatggcctgg ctcaccacag gctcgcgaac ctctaatgcg
      481 tgatgaaagt ccccacgacc agatcagcgt tggcggtgta acagcctatc tgaacctcga
      541 agttcttcct gatgttgagc tggtccgaga gatcagccgt gaacttgccg ttggcggcgg
      601 ccgtgccgaa ggtgaagccg tagtcaaggt agtccgggcc gagaacggcg acaacgtata
      661 ggccgttggc gaagcacgtg cctgtgacac gttcacttga caggttggcc gtaccgttga
      721 ttagcggaac ctggaacgtg ccgtctgaac caaggcccgg cgcgttgatc cagtcgccgg
      781 ccgcgatggc atacggctca gcgtcggcgt cgacgaactg gccgaagacc tggccgccgt
      841 agaacggatc tgcgaccgcg tcccctgtcg ccacctgagt ggagccctgc gtgacgatga
      901 tgccaaggtg ggcattgggc ttgaaggttc cggcgaactg ggcttcgccg atggccacta
      961 tcaggccggg gacgaacgtc cggcggtgaa cttggacggc accgccggct gacctccacg
     1021 tcgcctctgc cgtcgctccg ccgatcaggt cgatgccgtc ggtcgtgaag tcgaacgagt
     1081 aggccccacc cgcgctggtg acgacgctct gcgtgacgtc gtattcgggc aggccgaccg
     1141 tggaggcgcc cgcgctcgaa accgcgatcg tgatcatgct acccgcggga gccttgcctg
     1201 aaacgacctc gctgaccctg ttcgcatcga tcgtcagctt gggcaccgtt aacttgcgcg
     1261 tctgaccggt ttccacgacg agcacgttga tcttgtcgcc ggcagtgatg gtgccgctgt
     1321 agccgcacac gccccattcg ccgtacggac cggtgtcgac cgcttcgcgc atcttcatgt
     1381 tgcccgccgg atcgcgcacg acgaccttga cgaaggcgtt cttcctggcc tcgccggaga
     1441 cgcagctgtc gcccaagagc acatcgacgg tcagtggtcg cggagcggcc attgcgccgg
     1501 gtgcccagac cgttagcagc agggcggcgg caacgccgcc tgccatcagg cgacgcagcc
     1561 agcctcgagc gcgcgcgcga ccggttgagg aaagcttgga catggccagc tccttgattg
     1621 gtccggatca gcgcgctccg cggggaatgg cggacatcag acgcgatcgc atgcaattgt
     1681 tggttgccgc cattgcggcg atcacgggcg ttcggcccct aaaggcgtgc cgtttgaacc
     1741 ccgcccgcga aggccgaccg ctcagcgcgt catgaactcc tggctgacct cgtcgccctc
     1801 tgccgagtag cacaggatgc cgacgcggaa gttcttcttg atgtcgacct ggtcgcccag
     1861 gctgaccgtg aagctgccgt cggcgttggc ggtgccaaag ccgaagccgt agccgggctc
     1921 gctgatgccc tgcgcgagga tgacgaagcg gccgttcgcg aagcacttgc cggagacgct
     1981 gtcggccgcc acgttagccg tgcccttgat ggccggaatg cgccagccgg acaccgcgcc
     2041 gagcgccgga gcgttgagct tctcgccgcc cctgacgatg tacgtctcgc cgtcggcatc
     2101 gacgaaatcg ccgtagaacg agctgttgct gtatgagccg acgccattgc cacgggcgac
     2161 gatcgcgccg tccctggtga gcgtcatggc gatgggcgtg ttcggccccg ccgctccggc
     2221 aaagtcggac agacccagct ggagcacgat gtacggcgcg atctggaagc gcccgacggt
     2281 gaccgtgtcg ccaccgttgt gccagcggac caccagtgac gcgccaccct tgatgtccac
     2341 gccgtcgttg tcgaagtcat acgagtaggc gccgccgacc gcgaccgtgt gctgtacgac
     2401 gtcgtattgc tcgccccaca ggtcgaatcg gaagtcgaag gcctcgacct cgaccgtcgt
     2461 gcccgcgggt gccttgccgg agatcacgtt ggcgccgcgg tcgacctttg cggtcagcgt
     2521 gggtaccttg atgttgcgct tctggccggt ctcgaaatcg gtgaccttga tcgtgtctcc
     2581 cggcgacagc gggcggacgc cctgctgcat gcacgcctcc cagtagccat ccggcccggt
     2641 ctccaccgcg ccgcggccct tctgactgcc cgacttgtcg gtgatgatca ccttgaggaa
     2701 cgagtcgggc ttggccatgc catagacaca agagtcgccg atgaatagct cgaacgtgat
     2761 gggcggcggg gcggccagcg ccacgccggc gaacgagctc gcgaggacgc tacccagcag
     2821 gccggccagc acagctggct tcagggcaac acgaaacaga cccttggagc gcacgaattt
     2881 ccccttctag cgagagtgtt tagcggtagt cgccgtaatc gaccgacccg gatggggccg
     2941 gctggggccg cagcgcttcg acgcgcgccc ggcccagctt ctcgaggtac tcggtccggc
     3001 cactcacggc gtggacccat tcatcgagcc agcgggccaa tgacgcgcca tcgcgcgaga
     3061 tggtttccca ctcgaggtag aaggcgttgt cgcggtcgta gtagccctgg gcgtaggagg
     3121 ggtgtgcgcc gaacggctcg accaccacgg catcaaccac caacccgggc aggatggtcc
     3181 ggttagggtc ggcccggatc accgattcgt cgaccagttc ttcgacaacc acgatcaccc
     3241 gatcggcagc gaaggcagcc tccttctggc agcccagcag gccccacacc tgcgtgtcgc
     3301 ccgcagcatc ggcgcgctgc gcgtgcacga tcgttacatc cggcttcagg gccgggacgg
     3361 cgtacagctc ctcgtctgaa tagggcgacc tgatcggccg gatgagcggg ttagcaatcg
     3421 ggagatcagt gttttcgtag gaacgcagcg ggaagaacgg caggttcatc gcgcccgcgg
     3481 tgtagcggca gaccatgccg aagtggctgt attcctcgag ctcgagcttg ctgggaatct
     3541 cctcggccca ccgacggatc gcgtgcaggc tgcccacgcc cgggttgccc agccagctga
     3601 acacgagctt cctcgcaaca cccgcggcaa ccatctggtc gtagatcagg tcgggcgtga
     3661 gtcggcacag cgtcaggtca cgccgcccct ggcggatgat ctcgtggccg gcggcgaagc
     3721 cgatcaggtg ggtgaaaccc tcgatcgcga cgctgtcgcc gtcgcgcacg agctcggcta
     3781 tggcgtcgcg catggtcgcg accttggatg tgccgctcac gcgagtgacg gtagcgccgc
     3841 gagagggagc gggtcggacc tagccgtcga cctcgccgtc gtcgatggac atccagcggc
     3901 gcgactcgtc gtaggcgcgc gtcgggtcct tggccacctc cgcgaccgcg tcggtgaaga
     3961 ggcgcacgca ggtgtcgatc tcctgtgccg ttacgacgag gggtgggcac atccgtacga
     4021 cgctgtcacc ggcctcgagc acgagcaggc cgcgggtgaa gcacgcccac tggacggcct
     4081 cggcggtctc gcccgtgtcg aactccacgc cgagcatcag gcccttgccg cgcacgtccg
     4141 tgactagctt cgggtgctga cccatcagcg gtcgaagacc ggccagcgcc tgctcgccgc
     4201 gcgccgcggc gttgtccatc agcccctctt ggaggagctt gatcgtctca agcgcgaccg
     4261 cgcacgatac cgcgttgccg ccgaaggtcg agccgtggtg gcccttgccc cagctcatca
     4321 ggtcggcgcg ggcgatcatc gcgcccagcg gcatgccgct ggcgatgccc ttggccgtgg
     4381 cgacgatgtc gggctgtacg ccccagtggt cgatcgccca catcttgccg gtgcgcccgg
     4441 cacccgactg gacctcgtcg gcgatcagca ggatgccgtt gtcgtcgcag atcttgcgca
     4501 gccgcgcgaa gaaatctggc tccgggatga catagccgcc ctcgccctgg atcggctcga
     4561 cgacgatcgc ggcgatctcg ttggccggga tcaggcgctt gaagacgcgg ttctcgagct
     4621 cgtcgaggcc ggcgttgccg aacggcacgt ggtagacgcc cggcagcatg ggtccgaagc
     4681 cggcgtggta cttgctcttg ctcgcggtca gcgtgacggc gccgtacgtg cggccgtgga
     4741 agccaccgat gaacgcgacg aggtactggc gaccggttgc gaagcgcgcc agctttatgc
     4801 cggcctcgat tgcctcggcg ccggagttgc cgatgaatgc ccgggccggg cctttgatgg
     4861 gcgagatctc ggccagcttg gcggcaagct cagcgtagat gggcaggtag aagtcggacg
     4921 cgctgaagtg gatgaggtcg cgcgcctgct tctcgagcgc ggcagttatg cgcggatgcg
     4981 catagccggt cgaggagacg gcgatacccg ccgtgaagtc gagcagcagg ttgccgtcaa
     5041 tgtcctcgac cgtggaaccc gtgccgcgca cggggacgag gggataggcg cgcggcaggc
     5101 tgggcgaggt gaactcgtgg tcgaaggcga cgtgcttgcg cgccttggga ccgggtagcg
     5161 gctcgcggat gtcgggcacg ttgcgggctg tgccctggag ctggggacgc tcagccaact
     5221 ctgtcacttg ttcacccctc ggcggctacg cacccgctcc ggggcgctga atgcatagaa
     5281 acgatgcact gtgcatcaaa cgtgccaagt gtaccaagcg ccccgaccgc gtcgctcaga
     5341 tcggcttcag gcgagccatt atccggtcga tctcccggcc cctggccggg cgcgggaatg
     5401 caccttcgat cacgatcgga atccacgtga acgaatccac gcctgatgca ctcagggtga
     5461 cgtccaggat ggccgagtca ttctgcacac cactgaagta ggcgaagacg aagttgccca
     5521 ggctgtgtgg ttggccacgt cgacaccggc ggcgggcagc gcatcggcgg acgcgggccg
     5581 ggcacgaagg gtgtacttct caccgggcca cgacgtgcca cgatcagaga tggggcattc
     5641 gaggttgatc atgaacagct ccgcctgatc caggaagccg gccaccttgc gccagggcgc
     5701 atcggggccg ttcttgatga tgcgccgccc ggtgctctgc gcaagcacca cgtcgccgac
     5761 caccatgagc cggacggtcc ctgcggcgcc ggcgtgtccg tcgaaacggc gattggagtg
     5821 gctgcctcta acggacctgc ggtcgccgcc accatgaagg tgaccagcag gctcagggct
     5881 tgcggcggat cactggatgg ggctcggctc actggtgggc tcagcggtgg actccgcggt
     5941 cggctcagca gtgggctctg cggtcggctc agcggtgggc tccgcggtgg gggtgggcgt
     6001 acccgtcgga gtcggcgttg gcgatgggct cggattcgga tggctcagca tccagatgcg
     6061 aggtggctga ccgccgtcgt ggatctcccg cgcgatgtcg cggccaacgt acctgtagtg
     6121 ccacggttcg tagaggtagc acgtcgtctc gcgtgcgctc ttgggatagc tcaggatgaa
     6181 gccgtagcgc cacgcgttgg cggccagcca ccggctgatg ggtgtgcgct cccagcgggg
     6241 atagttggcc cagggcaccg gcccgcctgg tgacttgaag tcgacggcag tgcccagctg
     6301 gtgctcggag tggcctgccc tcgcgctgct caggagcgcg cgattcaagc cgatcttgcg
     6361 cacccactct cggaaggtgc tcgcctgtgc cccgtagctc cggtaggcgc tctggactgc
     6421 caggcggatc ccttgagccc gggcgtccgc ggccatcgct gacagatcgt cgatcaccaa
     6481 gccacgcacc ttgccgctgc cggggatgtg ggcgcggcgg acggacacga gatcgttggg
     6541 ggcgtatgcg ccggacaagc gccaccacgt gtcgattacc acgcggctcc agcgcccacc
     6601 gtcgtaggcc acgagctcat cgccggtgcg gcacggtggc gcatcgccgg ctctcgccgg
     6661 tgacgcgaag agcgtgagcg tgagggccag cgccaggacg gcgcgggcgc ggctccggtg
     6721 cagccgagat gagcgttccc cctggcccaa gttcggttgt cctttcggtc gggcggtgcc
     6781 cggtcagacg cctagtcgac gacggtttgt gactgctcgc gcatgaactg ctggatgtag
     6841 tacaggccgc cgccggcctt gccggttgag cccgagccct tccagccgcc gaagggctgg
     6901 acacccggcc aggcgcccgt cgtcgcgccg gcgcgccggt tcacgtacac gacgcccccg
     6961 tggatggtgt cgaggaactt ctgctgctcg gcctcatcct cactgtagaa gccggccgtg
     7021 agcgcgtact ccgattcgtt ggcgagcttg atgccctcat cgatcgagtc gaccggagcg
     7081 acggcgacga tcggcacgaa gagctcgtcg cggaacaggc ggtgcgtggc cggtaggccg
     7141 gtgacgacgg tcggcgccac gtagtagccg tggctcaggt caccgtcggt caggcgctcg
     7201 ccaccgaagg cgacgcggcc gtcgcgcttc gcttccgcga ccgcgttctc gtaccgcgcc
     7261 agcgccttgg tgttgatgat cggcccgagc cagttcgccc ggtcggacgg atcgcccacg
     7321 tgtatctggc gcgcgcgttc gacgagcttt tccacgaaag cgtcgtggac ctcacgctcc
     7381 acgtagacgc ggctgttggc ggagcacttc tggccgccaa acccaaaggc cgcacgcatc
     7441 acgccgtcgg ccgccttgtc cagatcggcg ctgtggctga cgacggtcgg gttcttgccg
     7501 cccatctcgg cgatgaccgg cttggggtag cgcttggcga agtgcttgta gaggtcgaag
     7561 ccgacttcga agctgccggt gaacacgatg ccgtcgacgt cgtccgactc cacgagctcc
     7621 gcaccgaccg tctcacccgg gccggtcacg atgttgaaga cgcccttggg cacaccggcc
     7681 tcgacgagga tctcgtacag cttcatggcc aggagcggcg cgtcactcga aggcttgcag
     7741 acaaccgtgt tgccggtcag gatggccgcg atcgatggcc cgccggccag cgccatgggg
     7801 aagttgaacg ggctcacgac cgcccacaca ccgtgcggct tgagcacgct gcgcgtccgc
     7861 accgtcgcgt cgccgagatt gtccatgggg tggtcgaagc cgttgttttc ctccatccgc
     7921 tcgctgtagt agcggcagaa gtcggccgtc tcctcgacgt cgcccagtgc ttcgagtcgg
     7981 ttcttgccga catcgaggac catcagcgcg gcgagctcca tctggcgcga gctgatgatc
     8041 tcggccgcgc ggcggatgat cgccacgcgc tcctgccagg gcgtgtcgcg ccaccccggg
     8101 aaggccgcgc gcgcggccgc gatcgcgtcc ttggcgtcct ggcgcgtgcc tttcgcgaag
     8161 tagcccacga tcaggctgtc gtcgatgggc gagtggtcct cgaactcgcc ctcaccctgg
     8221 cgcgcctcgc cgttcacgaa gttggggtag tgcttgccga gctgcgcccg cgccttggcc
     8281 atgccgtgct cgaattgctc gtgcagctcc tcgttgtcgt tgcgcagcgt cgcgtaggtg
     8341 atcttcaggc gcggccgctc gatcgtgtca gtcatgggaa ggcgtctcct cttgatgtcg
     8401 gctgaaagtc tacgcccgct ccgcgctggc caatcgtggc cgactggccc cttcgaaccg
     8461 cccccaatgg cacatccgcc gcgccatggc ccacactagg ttgcgcgcca actaccttcc
     8521 ggcggcgcca aaactggagc accaagagcc atgaccacgc tcaccccggc ggagcggccc
     8581 gatgcgggcc tcggccccga taccgctgag ctgcgcatcg gcgagcgcac gtacagcttc
     8641 cccatcgtgc gtggcacgga gggctacccg gcgatcaacg tgaccacgct gctgcgcgac
     8701 gcgggcatga tcacccttga ttacggcttc ggcagcaccg ccgcgacccg aagtggcatc
     8761 acgtatctcg atggcgacgc gggcgtcctg cgctaccgcg gctatcccat cgagcagctc
     8821 gccgagggtt cgaccttcct cgagaccgcg tacctgctca tgtacggcga gctgcccagc
     8881 ggcgagcagc tcgcggcgtg gactgacctc gtgacgcgcc acacgctgtt gcacgaggag
     8941 ttcaagcgct tcttcgaagg cttccccatg gacgcccatc ccatgggcat tctcgcggca
     9001 gcggtagcgg cgctgtcgac cttctaccag cagtccatgg acccacgcga cgcggacagc
     9061 gtcgacatca gcaccgtgcg cctgatcgcc aagatgccga ccatcgccgc gctcgcgtac
     9121 aagcactcgg tcggccaacc gttcatctac ccggacaact cgctcgacta cgagagcaac
     9181 ttcctgcgca tgatgttcgc catcccggcc gagccgtacc gcatcgaccc ggacgcggcg
     9241 cgcgcgctgc gggtgttgct catcctgcat gctgatcacg agcagaactg ctccacggcg
     9301 accgtccgac tggccggctc cagccacgcc aacctctatg cctctatctc ggccggcata
     9361 tccgcgctgt gggggccgtt gcacggcggc gcgaaccagg aagtgctcga gatgctgcag
     9421 cagatcgagg ctgacggcgg caacgtcgcc aagtgggtcg accgcgccaa ggacaagaac
     9481 gactcgttcc gcctgatggg ctttggccac cgcgtctaca agaactacga cccacgggcg
     9541 cgcatcatca aggcggccgc ggacacgatc ctggccaaac tgggtgccga agatcggctg
     9601 ctcgagatcg ccaaggagct cgaggcggtc gcgctgaccg atccgtattt catcgaccac
     9661 aagctctatc ccaacgtcga cttctacagc ggcctgatct accgcgcgtt gggcttcccg
     9721 acagagatgt tcaccgtgct gttcgcgatt ggccggctgc cgggctggat cgcgcagtgg
     9781 cgcgagatga tgaccgaccc ggattcgcgg attggccggc cgcgccaggt gtacaccggc
     9841 gcgcgcgagc ggaagtacct gcctctcgat cagcgctagc ggagccgcgc ggcaaagctg
     9901 gcgcgcgctg ccgcgcgggc ggagcgcccg gttcgctggc tc
//
LOCUS       DATBRX010000217         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3443449, whole genome shotgun sequence.
ACCESSION   DATBRX010000217 DATBRX010000000
VERSION     DATBRX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3443449"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..579
                     /locus_tag="VIF08_08315"
     CDS             181..579
                     /locus_tag="VIF08_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8136026.1"
                     /translation="MLTDAMAVATIPVTDLDRSLQFYRDVLGLQVLDENPAAIRLGAG
                     AGTQISIFRRPPVERGHTMAHFEVPDVEQTVADLRSRGVTFEEYASGPIATTNGIADY
                     APGLRGAWFKDPDGNVLGLREGPIPGRTQE"
     gene            576..1616
                     /locus_tag="VIF08_08320"
     CDS             576..1616
                     /locus_tag="VIF08_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136027.1"
                     /translation="MSSFREAVLASGNSLSDWKGFSVFGAGHHGGLPTPFDAPAGNRL
                     WGPQGSHPLAAIGLEELVAKSSNGIDGEITIKEPVRIGEALAGHIKLTARKDINARSA
                     AFRLMGALLVERNQSREERDSKGNVTKSEQWTEINGSVFDDLALMDTPLPAQLTAGQT
                     FEADFNFPAPRLGPVTAHMGSAAIVWALDAKWDVSMGGDERVSALIDVRQNIDYLRSG
                     AVRLDDGAMFDAYGSGDATIAVKPLPPIAAGTEVEVTVNWPGAGAGRGGRLELQADVD
                     APNKLSGVVLFSQVVDPNAFKAGYTTKITIPADAPAVLNDKGVAVHYRIRALVDRAFR
                     SDLAVERFIAVM"
     gene            1733..2449
                     /locus_tag="VIF08_08325"
     CDS             1733..2449
                     /locus_tag="VIF08_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136028.1"
                     /translation="MTFRRYRLWPIHLIAVLAVGCQTGTPPTLEPETIPDAIAGRYYE
                     VAFTASGGATVTTPNLPSNGLRLVTGSGHALLFGVPRDAGDITFTVTAIVGGPTMFQT
                     PASTQRTYSITINPANGDSGLVLADAAVGFTANSPLSLDFRDSIRGGTAPYTFALYCL
                     DSGSICTDPGPTDRPPDGVTLSAEGILAGVVGPSTGVYANRNDRRFDFIACVTDASSA
                     HACAVVEARESGPPASPTAT"
BASE COUNT          442 a          876 c          842 g          415 t
ORIGIN      
        1 cttcagcccg cgccgggaag tcggcctcgt tgacgccatg cgcgatgccc tgcgggacta
       61 cgcagcgttc gtcagcgcgc tctccgtcga gtggcttccc gccacgggcg gagcgggccg
      121 gctgtttggc tcgataatcc gacgcagaac cacccgcgac tcagcctgag gagagtgcaa
      181 gtgctgactg acgccatggc cgtggcgacc atcccggtca ccgatctcga ccgctcactc
      241 cagttctatc gcgacgtcct gggcttgcag gtgctggatg agaaccccgc tgcgattcgt
      301 ttgggcgctg gagccggcac gcagatctcc atcttcaggc gaccacctgt tgagcgcggc
      361 cacaccatgg cccacttcga agtgccggac gtcgagcaga ccgtggccga cctgcgctcg
      421 cggggcgtca cgttcgagga atacgcgtcc gggccaatcg cgacgaccaa cggcatcgcg
      481 gactacgcgc ccggcctgcg cggcgcctgg ttcaaggacc cagacggcaa cgtgctcggc
      541 ctgcgcgagg gcccgatccc gggaaggacg caggagtgag cagcttccga gaggcggtcc
      601 tggcgagcgg caacagtctc agtgactgga agggtttcag cgtcttcggc gccggccacc
      661 atggcggcct gccgacgccg ttcgacgcac cggcgggcaa tcggttgtgg ggcccgcagg
      721 gcagtcatcc cctcgcggct atcggcctcg aggagctggt cgccaagtcg agcaacggca
      781 tcgacggcga gatcacgatc aaggagccgg tcaggatcgg cgaagcgctg gccggccata
      841 tcaagctcac cgcccgcaag gacatcaatg cgcgcagcgc ggcgttccgg ttgatgggcg
      901 cgctcctggt cgagcgcaac cagtcgcgcg aggagcgcga tagcaagggc aacgtcacca
      961 agagcgagca gtggactgag atcaacggca gtgtcttcga cgacctcgcc ctcatggaca
     1021 cgccgctccc cgcacagctg accgcgggcc agacgttcga ggccgacttc aacttcccgg
     1081 cgccgcgtct tgggccggtg acggcgcaca tgggcagcgc cgcgatcgtc tgggcgctcg
     1141 atgccaaatg ggacgtctcg atgggcggcg acgagcgcgt gtcggcgctc atcgacgtca
     1201 ggcagaacat cgactacctg cgcagtggcg cggtccgtct cgatgacggc gcgatgttcg
     1261 acgcctatgg cagtggcgac gccacgatcg ccgtcaagcc gctgccgccg atcgcggccg
     1321 gcacggaggt tgaggtgacc gtcaactggc cgggcgcggg tgccggacgg ggtggccgat
     1381 tggagctgca ggccgatgtc gacgcaccga acaagctctc aggcgtcgtg ctgttctcgc
     1441 aggtcgtcga cccgaacgcc ttcaaggcgg gctacacgac gaagatcacc atcccggccg
     1501 acgccccggc cgtgctgaac gacaagggcg tcgcggtcca ctaccgcatc cgcgcactgg
     1561 tcgatcgcgc tttccggtcc gacctggcag tcgagcgctt catcgcggtg atgtagcccg
     1621 ctgggccacc atcccggcaa acgtatccgt gggtcaagga ccgcagagca gaacggtcgt
     1681 cgttacgaag ccttcgaccc ggcttgatcg cgttggcaat aatgtcggct caatgacgtt
     1741 tcgccgatac cgcctctggc cgatccatct gatcgccgtc ctggcagtcg gatgccagac
     1801 gggtacgcca cccacactcg agccggagac gatcccggac gccatagcgg gccgctacta
     1861 cgaggtcgca tttaccgcca gcgggggtgc gacggtcacg acgcccaacc tgccaagcaa
     1921 cggcctccgc ctggtcacgg gcagcgggca tgccctgttg ttcggcgtcc cacgtgacgc
     1981 tggcgacatc acgttcacgg tcacggcgat cgtcggcggg ccgacgatgt tccagacccc
     2041 ggcatcgacc cagcgcacct attcgatcac catcaatcct gccaatggcg acagtgggct
     2101 ggtcctagcc gatgcggcgg ttggcttcac ggcgaattcg cctctgagcc ttgacttcag
     2161 ggactcaatc cggggtggta ctgcgcctta caccttcgcg ctctattgcc tcgattccgg
     2221 ctcgatctgc accgatccgg ggcccacgga ccgaccgcca gatggcgtga cgctgtcggc
     2281 cgaaggcatc ctcgctggcg tcgttggtcc atccaccggc gtgtacgcaa accgcaacga
     2341 ccgacggttt gacttcatcg cctgcgtcac agacgcctcg tcggcgcacg cttgcgcggt
     2401 ggtcgaggca cgcgagagcg gcccgcctgc gtcgccaacc gctacttgag cagcatcttg
     2461 cccatgcggc gggatgcgat gtacacgaag agcaggccca tcgcgaacag gtagaccatg
     2521 tcgatgagtt gcgcgacgcc gatcacgccg gtcgtcatcg cgcgcaccag gtcca
//
LOCUS       DATBRX010000218         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3220986, whole genome shotgun sequence.
ACCESSION   DATBRX010000218 DATBRX010000000
VERSION     DATBRX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3220986"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VIF08_08330"
     CDS             complement(<1..295)
                     /locus_tag="VIF08_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136029.1"
                     /translation="MIVTIAGRPLSLDPRPIWRALRADIAAMPRALKAWLLFLLALMG
                     IGAIAAVRALFPGDKGIATTPTVEWGLLIIGYVFFAITTSGLCLASSLGTVFGI"
     gene            complement(292..1128)
                     /locus_tag="VIF08_08335"
     CDS             complement(292..1128)
                     /locus_tag="VIF08_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8136030.1"
                     /translation="MTDRLSVLGPISRRSLLKAAAVSVPCLIGGGAIFEALSTLASTP
                     VPTVDPMVANYDAAAHRWGFVVDTAACIGCGKCVVACKTENGVPFDGEHTRTWIERRT
                     LTTSGAVIVQAPDAGIDGFADAPLPVGASEVVDSKFVARLCMQCELSPCTTVCPVGAT
                     YRTADGVILVDEARCIGCGYCVVACPYGARYLVPAGARTPTGVAGVADKCTFCYHRVT
                     RGQQPACVEVCPVQARKFGDLNDEASEVSVILRDQATRVLRPELGTKPRVHYLGLEDR
                     LA"
     gene            complement(1125..1691)
                     /locus_tag="VIF08_08340"
     CDS             complement(1125..1691)
                     /locus_tag="VIF08_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136031.1"
                     /translation="MATVLGAGAFAASGFYDQFGAPRTNVNARSWQLRPQVYATSDCR
                     GCHEDAAAATRGLAHERLMCEACHIPSVDHPGPVSGVVQMLPAPSESDCTTCHASLPG
                     RPAAFTQVVLDDHYAGAECLSCHDPHTSTATKPPEVTHPLVHLPACATCHAPLGLKSY
                     PVNHEPAPDNVCLGCHRLGAGVGGTGSQ"
BASE COUNT          335 a          729 c          764 g          277 t
ORIGIN      
        1 cgatgccgaa gaccgtgccg agcgacgacg ccaggcacag cccgctcgtg gtgatggcga
       61 agaagacgta gccgatgatc agcaggcccc attccacggt cggcgtggtg gcgatgccct
      121 tgtcgcccgg gaacagggcg cgcaccgcgg cgatggcgcc aatgcccatg agtgccagca
      181 ggaacagcag ccacgccttg agcgcgcgcg gcatggccgc gatatctgcc ctgagggcgc
      241 gccagatggg ccgcgggtcg agcgacagcg ggcggccagc gatggtgacg atcacgccag
      301 ccggtcctcg aggcccaggt aatggacgcg cggcttggtg ccgagctccg gccgcaggac
      361 gcgcgtcgcc tgatcgcgca ggatcacgct gacctcgctc gcttcatcgt tcaggtcgcc
      421 gaacttgcgc gcctggaccg ggcagacctc cacgcacgcg ggttgctggc cgcgcgtcac
      481 gcggtggtag cagaaggtgc acttgtcggc caccccggcc acgccggtcg gcgtgcgcgc
      541 gccggcgggc acgaggtagc gcgcgccgta cggacacgcc acgacgcaat agccgcagcc
      601 gatgcagcgc gcctcgtcga ccaggatcac gccgtcggcg gtgcggtacg tggcaccgac
      661 cgggcacacg gtggtacacg gcgacagctc gcactgcatg cacagccggg cgacgaactt
      721 gctgtcgacg acctcgctcg cgccaacagg caggggcgcg tcggcaaatc cgtcaatccc
      781 cgcgtccggt gcctggacga tgacggcgcc gctggtcgtc agggtccggc gctcgatcca
      841 ggtgcgcgtg tgctctccgt cgaacggcac gccgttctcc gtcttgcagg cgacgacaca
      901 tttgccgcag ccaatacacg ccgcggtgtc aaccacgaag ccccagcggt gggcggcggc
      961 gtcgtagttc gcgaccatcg gatcgaccgt cgggacgggc gtcgacgcga gggtcgacag
     1021 ggcctcgaag atcgcgccgc cgccaatcaa acagggcacg ctgaccgccg ccgccttgag
     1081 caggctgcgc cggctgatgg gtcccagaac gctgagtcgg tcggtcattg gctgccagtt
     1141 cctccgacac ccgcgccaag gcgatggcac ccaagacata cgttgtcggg cgcgggctcg
     1201 tggttgaccg gatacgactt caggccgagc ggcgcgtggc aggtcgcgca agccggcaga
     1261 tggacgagcg ggtgagtcac ctcgggcggc ttggtcgcag tcgaggtatg cgggtcatgg
     1321 cagctcaggc attccgcgcc ggcgtagtgg tcatcgagca ccacctgggt aaaggctgcc
     1381 gggcgaccgg gaagtgacgc gtggcacgtc gtgcagtcgg attcactggg cgccggcagc
     1441 atctgcacaa cgccgctgac cggacccggg tggtcgacgc ttggaatgtg gcaggcctcg
     1501 cacatcagcc gctcatgggc aaggccgcgg gtcgcggcgg cggcatcctc gtgacagccg
     1561 cgacagtccg acgtcgcgta gacctgtggg cggagctgcc acgagcgggc attgacgttg
     1621 gtgcgtggcg cgccgaactg gtcgtagaag ccgctcgccg cgaacgcgcc cgcaccgagg
     1681 acggtggcca ccaccatcat cgccgcgagg agcagtgccg ggcggcgcag ccgacggagc
     1741 gtcgaccgcg ccgcagcgac ggcctgacca gcagagcggc gcgccttgcg ggtcgccgcg
     1801 cgacgcctcg tcgcgagtgc ccgcctacgg ccgcgcgcgg cggagctcac gccgtccacc
     1861 gggacgggag ctcactcacc gcagctgagg cgcagcgcat cgatcggtcg ctccgcccgc
     1921 cttacccggc aggcagggcc cttccagcca acagcctatg gcgacgtcgg gcacggcccg
     1981 cagcgcaggc cggccccttt cgggggtccg ttggtcccgt gaaacggtac gtgcggccct
     2041 gaggggacgc ccgcgcgcgg caggaacatg cggccaggtc ctcgaaggga tccgctgcgc
     2101 acgca
//
LOCUS       DATBRX010000219         1751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2997628, whole genome shotgun sequence.
ACCESSION   DATBRX010000219 DATBRX010000000
VERSION     DATBRX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2997628"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VIF08_08345"
     CDS             complement(<1..371)
                     /locus_tag="VIF08_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8136032.1"
                     /translation="MSKSMLALLAGVLLLALACAAACAAPQAGQNGPTDARIASAEPF
                     TWDPARAGDAGTASVLAQVFEGLTAFDASGGVQPALAQSWSVSDDGRQITFQLRPGLL
                     YSDGNPIRAQDVVDSWLALLNP"
     gene            436..852
                     /gene="msrB"
                     /locus_tag="VIF08_08350"
     CDS             436..852
                     /gene="msrB"
                     /locus_tag="VIF08_08350"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY8136033.1"
                     /translation="MKTFDPAELRARLTPLQYSVTQNADTERPFTGVYLDNHAEGTYR
                     CVVCREPLFESGTKFESGSGWPSFYDVAQHGRVAQTTDTSHGMTRTEVTCANCGAHLG
                     HLFPDGPRPTGLRYCINSASLDFEPVPPAGRAADEG"
     gene            1070..1453
                     /locus_tag="VIF08_08355"
     CDS             1070..1453
                     /locus_tag="VIF08_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8136034.1"
                     /translation="MPRDPNIPDEFQVYTLCLLRRPKDAPQLKEEWRDELQTQHLAYR
                     AELRQRGVLVANGPFREQTDVALRGLSIFTTGLEETARLNDGDPMVKAGWLGYDLMEW
                     WVASGTLAFPNSAGEIGDRRVMEVD"
     gene            1453..>1751
                     /locus_tag="VIF08_08360"
     CDS             1453..>1751
                     /locus_tag="VIF08_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136035.1"
                     /translation="MDYLPRVGVIVLAFALLACGPEAATTPMAVDLLTESQASLACMD
                     ALASGRLVADPRSGLGISASGGERYSVMWPFGYSAVYADDRLWLLARDGTPIAAEG"
BASE COUNT          291 a          593 c          586 g          281 t
ORIGIN      
        1 gggttgagca gggcgagcca cgagtcgaca acgtcctggg cgcgaatggg attgccgtcg
       61 ctgtacagga gtccgggacg cagctggaag gtgatctggc ggccgtcgtc gctgaccgac
      121 cacgactggg cgagggcggg ctggacgccg ccgctcgcgt cgaacgcggt caggccctcg
      181 aagacctgcg ccaggacgct cgccgtgccc gcgtcgccgg ctcgcgccgg gtcccaggtg
      241 aacggctccg cggatgcgat gcgcgcgtcg gtggggccgt tctgcccagc ctgcggcgcg
      301 gcacatgccg cggcacatgc aagggcgagc aacagcaccc cggcaaggag cgccagcatg
      361 gacttcgaca cggagtccag agtctagcgg cgctgcgcgc ccatccgtgc gagcggcact
      421 aacgtatctc tggacatgaa gacattcgat cccgccgagc tccgcgcccg attgacaccg
      481 ctccagtact cggtcaccca gaacgccgac acagagcgtc cattcaccgg cgtttaccta
      541 gacaaccatg ccgagggaac gtatcgctgc gtcgtctgcc gtgagccgtt gttcgagtca
      601 ggcaccaagt tcgaatcggg ctcgggctgg cccagcttct acgacgtcgc ccagcacggt
      661 cgcgttgccc agacgaccga cacatcgcac ggcatgaccc gcacagaggt gacatgcgcc
      721 aactgcggcg cgcacctggg tcacctcttc cccgacgggc cacgcccgac cgggctgcgc
      781 tactgcatca actcagcgtc gctcgacttc gagccggtgc ctccggcggg tcgggcggca
      841 gacgagggct gaccgcggcc tgaccgcggc ctgaccgacg gttgatccgc tgagcggttg
      901 aaccgtccgg cgaacgccca cggcgctgtc gatatcgggc cttcacagga tgtttgagtc
      961 gcccagcggt tgaatcgacg gctggagaac gttcgtcggc gtctagcgcc ctcgccagcg
     1021 gacgattgac ccgttgagcg cataaaggac cgccttacac tcgcggccta tgccgcgcga
     1081 ccccaatatt cccgacgagt ttcaggtcta cacgctgtgt ttgctgcgcc gcccaaagga
     1141 cgcaccgcag ctcaaggaag agtggcggga cgagctgcaa acacagcacc tcgcctatcg
     1201 tgcggaactg cggcagcgtg gggtcctcgt cgccaatggg ccgttccgcg aacagactga
     1261 cgtagcgctg cgcggcctgt cgatcttcac gaccgggctc gaggagactg cgcggctcaa
     1321 tgacggcgat cccatggtca aggccggctg gctcggctat gacctcatgg agtggtgggt
     1381 ggcctccggc acgctcgcgt tcccgaattc cgccggcgaa attggcgacc gccgcgtgat
     1441 ggaggtcgac tgatggacta cctgccgcgc gttggcgtga tcgtgcttgc cttcgcgctg
     1501 ctcgcatgtg gcccagaagc ggcgactacc ccgatggccg tcgatctgct gacggagagc
     1561 caggcctcac tggcatgcat ggacgcgctg gctagcggcc gactcgtagc cgacccccgc
     1621 tccggcctgg gcatctcagc gtccgggggc gagcggtaca gcgtgatgtg gcccttcgga
     1681 tactcagccg tgtacgcgga cgatcgcctc tggctgctgg ctcgcgacgg aacgcctatc
     1741 gccgccgagg g
//
LOCUS       DATBRX010000220         6357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3891740, whole genome shotgun sequence.
ACCESSION   DATBRX010000220 DATBRX010000000
VERSION     DATBRX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3891740"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VIF08_08365"
     CDS             complement(<1..546)
                     /locus_tag="VIF08_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136036.1"
                     /translation="MTGQTAALRESVAPEAVRRALAGNKLQLGQIWFQALLMGALLVA
                     LGVLVSLLIDVFDRGGAMLAERGVDFLTANTSSNPAIAGVGQGIFGSLLIVAFVLVFA
                     FPVGIGAAIYIEEYARDTFLTRVLNAAVRNLAGVPAIVYGLLGLAVFVIILSSVTGGR
                     SVIAGGLTMAALVMPIVVITTA"
     gene            complement(566..1507)
                     /gene="pstC"
                     /locus_tag="VIF08_08370"
     CDS             complement(566..1507)
                     /gene="pstC"
                     /locus_tag="VIF08_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY8136037.1"
                     /translation="MTGAQLASPISLTDLRGSPARRRRERLIRSLFLGAAAVSLVISA
                     LIVLTLLGRALDFISRVDLADLISVGWLPRRGYFDIATIFAGTLVITAIGMVIATPLG
                     LGAAIYLSEYASPRRRRVLKPIIELLAGIPSVVLGFYAIAFLAPTIVQPLCGSNSIFS
                     MGAAGLGVGILITPLIASVAEDAMHAVPMSLREASYGLGARKRSTSLRVVLPAAVSGI
                     VAALILGVSRGIGETMVVSIAAGAVGGSVFTVNPCLPGQTMTAAMTALATGSDQAQVG
                     ADLAVPSLFFVGLLLFAITLGLNLVSERFVRRVRKAY"
     gene            complement(1609..2583)
                     /locus_tag="VIF08_08375"
     CDS             complement(1609..2583)
                     /locus_tag="VIF08_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8136038.1"
                     /translation="MPSPSSLPSGQGVSGEIFVSGSSTVQPISQAVSEEFKTANPDFG
                     FTVEGPGTGPGFAAFCAGDSDVSDASRKIKADDPAKEGDEEATVCANNGVEYTELKIA
                     YDGITVMTHPDTPVDCLTKADLYALFGPESDTFANWSDAQALATELGSTTTLPDLALQ
                     ITAPGEESGTYDAFLELSGITSTGTTRGKFDDTHPAALRLPGPNYTASPDDNVIVAGV
                     EAGAGSLGFVGFAYFEGAGTAAKAIAIDGGDGCVTPTLETIADGSYPLSRGLYIYVNN
                     AKADENAALQAWTDFYLSDTGIANVTEVGYVALPADELQATRDAWTNR"
     gene            3088..4233
                     /locus_tag="VIF08_08380"
     CDS             3088..4233
                     /locus_tag="VIF08_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136039.1"
                     /translation="MKERDRHMRSWIALAVMVVAIASPAIALGATATYAPESWFKDYI
                     RVTRRTTNDSVKIGIHPRWNAAGAAAIQGYAADGYRYTQDMHDLHYVLDAYGYYTDFP
                     NPVWDTDDDEPFGSDWKYEESEMTSNSTTFPTVMRNYVFQTKWSHWFFNPCCGYPDWY
                     WDGRSGEIAHESQISFYLGEWQTHKHSGAYDVSTYPAVSKPSAPTQTVALSTSEALAT
                     GSDWAVEAEHRVLLDPDLSEGIDRYRSSATDLAERTVGSEPSHVVVTFDRPLDPSDVL
                     ALMSAGLEIRTIELAAMQGDIHVTYGGRVFDGSELNALLADAGADSATVLGFVSAEAL
                     ITSAGYQGVSTDNRVYLIDFALEAWMREHPGSKAILDDVFWQRAGWE"
     gene            4383..4736
                     /locus_tag="VIF08_08385"
     CDS             4383..4736
                     /locus_tag="VIF08_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136040.1"
                     /translation="MLDPDLRSEAFDNDEERYKDLARQAEWSRFLWSIDGVGADGSAW
                     QYAYLALPGGPDSIPSFLANANGLSIVNVLKPTADARRARVAIRRDASGEWWVYAYDV
                     VMIPSFEPVPTDLSP"
     gene            complement(4750..>6357)
                     /locus_tag="VIF08_08390"
     CDS             complement(4750..>6357)
                     /locus_tag="VIF08_08390"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY8136041.1"
                     /translation="EGRELELKYAVTDRAAVEDIVSANVVFGLDAGEWREHDVVDQYL
                     DTRGGALARAGYGARLRRLGGRTMVTVKSIARRQGPGRGRAGALHDRLELEARATRQL
                     DPGRWPESTARAMIEVSAGGEALHTLFTIEQHRRERDLLRDGGVVATLSLDSGVVKRF
                     GRPLGAIDALEIESTAAHPAEARRVLAAIAADVETVPTLRPEERSKEELAHALVERAL
                     SDAKPVRPPQKPGIGADDSLAEAGRKVLRMHLLRMLAAEPGARLGEGTEGVHKMRVAT
                     RRMRAAWRVFDGAYPGRVARRHVAELREVAQALGTVRDLDVMLERLAAHRAGLASEEA
                     KAALEPLVAEWRGNRSVARRELLDLLQGAAYDHFVTDYRELVDAPAVSASAPDEPVTR
                     LRDVAAGRIWRAYERLRAHDAAVPFADVPALHALRVDGKRFRYTLEFLREILPPTVEP
                     IVAEVTKLQDHIGELNDSQIAAETTRAWLIDAAGGLNPAEKRAAAAYLRSAERDVLRL
                     TRAFPALWRRITGPTFRRRLALVIGAI"
BASE COUNT         1085 a         2082 c         2157 g         1033 t
ORIGIN      
        1 agcggtggtg atgacgacga tgggcatcac cagcgcggcc atcgtgaggc cgccggcgat
       61 gacgctccgc ccgccggtga cggacgacag gatgatcacg aagaccgcca ggccgagcag
      121 gccgtagacg atcgccggca cgccggcgag gttgcgaacg gcggcgttca ggacgcgcgt
      181 caggaacgtg tcgcgcgcgt actcctcgat gtagatcgcg gccccgatgc ccaccgggaa
      241 cgcaaacacc agcacgaagg ccacgatcag cagcgagccg aagatgccct ggccgacgcc
      301 ggcaatcgcg ggatttgacg atgtgttggc agtcaggaag tcgacgcccc gctcggcaag
      361 catggccccg ccgcggtcga aaacgtcgat cagcaggctc acgaggacgc ccagtgcgac
      421 gagcagcgca cccatgagca gcgcctggaa ccagatctgg cccagctgca gcttgttgcc
      481 ggcgagcgca cggcgcacgg cttcaggtgc aacggattca cgcagcgcgg cggtttggcc
      541 ggtcatcgca gcggcgcttt cactgctagt aggccttccg gacgcggcgc acgaagcgct
      601 cgctcaccag gttcaggccg agagtgatgg cgaagagcag cagtccgacg aagaagaggc
      661 tcggcaccgc caggtcggcg ccaacctggg cctggtccga cccggtcgcg agtgcggtca
      721 tcgccgcggt catcgtctgg ccgggcaggc acggattgac cgtgaatacc gagccaccca
      781 ctgccccggc ggcgatggac acgaccatcg tctcgccgat gccgcgcgag acgcccagga
      841 tcaacgcggc gacgatgccc gacacggccg ccggcagcac cacgcgcagg ctggtcgagc
      901 gcttgcgcgc acccaggccg taggaggctt cgcgcagcga catcggcacg gcgtgcatcg
      961 cgtcctccgc caccgacgcg atgagcggag tgatcaggat gcccacgccc aggccggccg
     1021 cgcccatgga gaagatggag ttgctgccgc acagcggctg gacgatggtc ggcgcgagga
     1081 acgcgatggc atagaagccc aggaccacgc tggggatgcc ggccaggagc tcgatgatcg
     1141 gcttcaggac gcgccggcgg cgcgggctcg cgtactcgga caggtagatc gcggcaccca
     1201 ggccgagcgg cgtggcgatg accatgccga tcgccgtgat gacgagcgtg ccggcgaaga
     1261 tcgtggcgat gtcgaagtag ccgcgccgcg gcagccagcc cacggagatg agatcggcga
     1321 ggtcgacccg cgatatgaag tcgagtgccc gtcccagcag cgtcaggacg atcagcgcgc
     1381 tgatcacgag cgacaccgcg gcggcgccca ggaagagcga tcgaatcagc cgctcacgcc
     1441 gccgccgcgc ggggctgccg cgcaggtccg tgagcgaaat tggggaagcg agctgggcgc
     1501 cggtcacttc ggtcagtgtc ctacacaact acagggctcg ggcggaaagg gcatggggcc
     1561 ggcacaaggc cggccccatg tggggaggag gagcggtttg ggcgggagct agcggttcgt
     1621 ccacgcgtcg cgcgtggcct ggagctcgtc ggccggcagg gcaacgtaac caacctcggt
     1681 gacgttggcg atgcccgtat ccgacaggta gaagtcggtc caagcctgga gggccgcgtt
     1741 ctcgtcggcc ttggcgttgt tgacgtagat gtacaggccg cggctcaagg ggtacgagcc
     1801 atcggcgatg gtctcaaggg tgggcgtgac gcaaccatca ccgccgtcaa tggcgatggc
     1861 cttggcggcc gtgccggcac cctcgaagta ggcgaagccg acgaagccca gcgaaccggc
     1921 accggcctcg acgccggcaa cgatcacgtt gtcgtcgggc gaagccgtgt agttcgggcc
     1981 gggcaggcgc agcgccgccg gatgagtgtc atcgaacttg ccgcgcgtcg tgcccgtcga
     2041 ggtgatcccc gacagctcga ggaacgcgtc gtaagtgccc gactcttcgc ctggtgcagt
     2101 gatctgcagc gccaggtcgg gcagagtggt ggtcgagcca agctcggtcg ccagcgcctg
     2161 cgcatccgac cagttggcga aggtgtccga ctccgggcca aacagagcgt agagatcggc
     2221 cttggtcagg cagtcgacgg gcgtgtcggg gtgggtcatg acggtgatgc cgtcgtaggc
     2281 gatcttgagc tcggtgtact cgacgccgtt gttggcgcag accgtggcct cttcgtcgcc
     2341 ttccttcgcg ggatcgtcgg ccttgatctt gcgcgacgca tcgctcacat ccgaatcgcc
     2401 agcgcagaac gcggcgaagc ccggaccggt gcccggtccc tcaaccgtga agccgaagtc
     2461 gggattggca gtcttgaatt cctctgacac ggcctgcgag atgggctgga ccgtggagga
     2521 gccggagacg aagatctcgc cgctcacgcc ctggccgctc ggcagggatg acggactcgg
     2581 cacggatgac ggattgctcg ttgcgccgct tgtaggggtc ggggcggcag ggccgccgca
     2641 cgcggcaacg agagcggtgg tggcgatcgc tagagccgcg atcgtcttgc cggtgataac
     2701 ctggatgact ctcctctctg gggcacggac gtgcctcttg cgaggacgag tctggaagtg
     2761 gcaggtgaac gtacctttgg cgccgggtta acggtctgtt aactgtttag tcgcacgttc
     2821 gagtcctagc gccgaaactg tttcacgcct gcttagccga gcgggtatcg atgtcggagg
     2881 ccaaaccgga cccaggctgc agtcccacga gcacgtgagt gccaaatggt caactgacga
     2941 cgtgacattc ggctctcgtg cccgcaacgc acaccgctct accgtttccg atcgactaaa
     3001 gcatgttcgt ggccccagcg ccgcggcagt cgtacgttgg ctctgtccat gacacccgcg
     3061 atagcagcag gcagccgcgc cctaacgatg aaggagcgcg accgtcacat gagaagttgg
     3121 atcgcacttg cagtcatggt tgtcgccatt gcctctccgg cgattgctct cggtgctacg
     3181 gctacttacg ccccggagtc atggttcaag gattacatcc gcgtcactag gcggacgacc
     3241 aacgattcag tcaaaatcgg aatccatcct cgctggaacg cagctggtgc agccgcaatt
     3301 cagggctacg cggcggacgg ataccgatac acgcaagaca tgcacgatct ccactacgtc
     3361 ctggacgcgt acggctacta cacggacttc cccaatccgg tatgggacac cgacgatgat
     3421 gagccatttg gatcggactg gaagtacgaa gagtcagaaa tgacgtcgaa ctcaacgacc
     3481 tttcctaccg tcatgcggaa ctacgttttc cagacgaaat ggtcccattg gtttttcaac
     3541 ccgtgctgcg gttacccaga ctggtattgg gatggccgat cgggcgagat tgcccacgag
     3601 tcacagatct cgttctactt gggcgaatgg cagacacata agcattcggg cgcgtacgac
     3661 gtgtccacct atccggctgt gagcaaaccc tccgcgccga cccagacggt cgcgctatcg
     3721 acctcagagg cactagcgac aggttctgac tgggccgtgg aggcagagca tcgagtactt
     3781 cttgatcccg acttgtcgga gggcatcgac cgctatcgct cgtccgcgac cgatctggcc
     3841 gagcggaccg ttgggtcgga gccgtcgcac gttgtcgtga catttgaccg gcctctcgac
     3901 ccctcggatg tgctcgctct gatgagcgcg gggcttgaga tccggacgat cgagctcgcg
     3961 gcgatgcagg gcgacattca tgtcacttac ggcgggcgag tcttcgacgg ttccgaattg
     4021 aacgcgttgc tcgcggacgc cggcgcagat agcgcgaccg ttctgggttt cgtttctgct
     4081 gaagcgctca tcactagcgc cgggtatcag ggtgtgtcaa cagataaccg tgtctaccta
     4141 attgattttg cgctcgaggc ttggatgcgc gagcatcccg gcagtaaggc cattctcgat
     4201 gacgtgttct ggcagagggc cggttgggag tagcaacacg cgcggtaatc ctcatcgcag
     4261 cctgccttct cggcgccctg gcaggttgtg tcggcgttcg cacggagggc ccgggcgttg
     4321 gcgggatggt tgatgcgtac ctgagatcgg ccgccggtgc ggctcctgat cgaggttggg
     4381 caatgctcga tcccgacctc cggagcgagg cgttcgacaa cgacgaggag agatacaagg
     4441 acctcgcccg acaagcagaa tggtcgcgat tcctgtggtc aatcgacggc gtcggggcgg
     4501 acggctcggc ttggcagtat gcgtacctcg ccttgcccgg aggcccagac tcaatcccct
     4561 cattcctcgc gaacgcgaac gggctttcga tcgtcaacgt gctcaagcca actgccgatg
     4621 cacgcagagc gcgcgtcgca attcgacgcg atgcgtctgg cgaatggtgg gtctacgcat
     4681 atgacgtcgt catgattcca tcgttcgaac ctgtccccac cgatctctcg ccctaagcgc
     4741 gccgatcgat cagattgccc cgatcaccag cgccaggcgc ctgcggaagg ttggtcccgt
     4801 gatgcgccgc cacagcgccg ggaaagctcg cgtgagccgg aggacatcac gttcggccga
     4861 tctgaggtac gccgcggcgg cacgcttctc ggccgggttc agcccgcccg ccgcgtcgat
     4921 gagccaggcg cgcgtcgtct ccgctgcgat ctggctgtca ttgagctcgc caatgtggtc
     4981 ttgcagcttc gtcacctcag ccacgatggg ctcgaccgtc gggggcagga tctcgcgcag
     5041 gaactcgagc gtgtagcgga agcgcttgcc gtcgacgcgc agcgcatgca gcgcgggcac
     5101 gtcggcgaac ggcaccgcgg cgtcgtgggc gcgcaggcgc tcgtaggcgc gccagatgcg
     5161 cccggcagcg acgtcgcgca ggcgagtgac tggctcgtcg ggcgcgcttg ccgacacggc
     5221 gggcgcgtcg accaactcgc ggtagtcggt gacgaagtgg tcataggccg ctccctgtag
     5281 gaggtcgagc agctcccggc gcgccacgct tcggttcccg cgccactcgg ccacgagcgg
     5341 ctccagcgcg gccttcgcct cttcgctggc caagcctgcg cggtgagcgg ccagccgctc
     5401 cagcatcacg tcgaggtcgc gcacggtgcc gagcgcctgg gcgacctcgc gcagctcggc
     5461 cacgtgccgc cgcgcgacgc gtcccgggta cgcgccgtcg aacacgcgcc acgccgcgcg
     5521 catgcgtcgc gtcgcgacgc gcatcttgtg cacgccttcc gtgccctcgc ccaggcgcgc
     5581 gccgggttcc gcggcaagca tgcgcagcag gtgcatgcgc agcaccttcc gaccggcctc
     5641 ggcgagcgag tcgtcggcgc caatccccgg tttctgcggc ggccggacgg gcttcgcgtc
     5701 cgacagcgcg cgctcgacga gcgcgtgggc cagctcttcc ttcgagcgct cctccggccg
     5761 gagggtcggc acggtctcga catcggccgc gattgcggcc aggacgcggc gcgcctctgc
     5821 gggatgggcg gcggtcgact cgatctcaag cgcgtcgatc gcgcccaacg ggcgcccaaa
     5881 acgctttacg accccggaat ccagcgacag ggtcgccacc acgccaccat cgcgaagcag
     5941 gtcccgctcg cgccggtgct gctcgatggt gaagagcgtg tgcagcgcct cgccgccagc
     6001 ggacacctcg atcatcgccc tcgccgtgct ctccggccag cgcccgggat cgagctgccg
     6061 cgtggcccgc gcctcgagct cgagccgatc gtgcagtgcg ccggcgcggc cgcgcccggg
     6121 gccctggcgg cgcgcgatgc tcttgaccgt caccatcgtg cgcccaccca gccggcgcag
     6181 ccgcgctcca tagcctgcgc gggccagcgc gccgccgcgc gtgtcgaggt attggtcgac
     6241 cacgtcgtgc tcgcgccact cgcccgcgtc taagccgaag acgacgttcg cggaaacgat
     6301 gtcctccacc gcggcgcggt ccgtgaccgc gtacttgagc tctagctcgc gcccctc
//
LOCUS       DATBRX010000221        18545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3556533, whole genome shotgun sequence.
ACCESSION   DATBRX010000221 DATBRX010000000
VERSION     DATBRX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 18545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18545
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3556533"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VIF08_08395"
     CDS             complement(<1..834)
                     /locus_tag="VIF08_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136042.1"
                     /translation="MADDVRAETERLVTEAASRINEGATNDAVVTWLRAQASVAEVEG
                     DADAIRFRPAGGRGVWIVPPPIVTSQAVALRSKAAFGAGTFATSPPAGREQIVAGAGQ
                     SLRRARVLSPFRWDFGGSDDGQAVAERLAQLPDYANGVEYAENTNVADSDVTVDDFRG
                     WQGLQVVHIVSHGFRLCNDGKCRAVVAANALLTGVANIWPSAVRGLELEVVAGAGAAP
                     QRPRFFSLLGADFFRDQYPGGLTDAVVFLNGCATFGDGATDLGNAIRGSTSVVLGWSR
                     SV"
     gene            complement(1104..1751)
                     /locus_tag="VIF08_08400"
     CDS             complement(1104..1751)
                     /locus_tag="VIF08_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136043.1"
                     /translation="MTDRSAGREPQTRDDGDDAQGGWRRVARSVLTLRWVPLAAALLS
                     LPLSAAAILISLQQPEVLVILPDQVRVAQGHQSGAAYLYLQPAFVSTGRSERIEVIRD
                     MRLEVSGPGEPTDLEWTQQLRLVSQPGGGLSYEYVGDAVPLLVSPRDAAAPLALFEAP
                     QGWFFTAGDYTFKLVADRVITGQPLTDEFTVSLDDADIQFLDEPGTERFLTFPID"
     gene            complement(1748..3145)
                     /locus_tag="VIF08_08405"
     CDS             complement(1748..3145)
                     /locus_tag="VIF08_08405"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY8136044.1"
                     /translation="MTANPDPTPAVANLDTIVSLAKRRGFVFPSSEIYGGINAVWDYG
                     PLGVELKNNVKRAWWRAMVQERDDIVGLDAGILMHPQVWVTSGHVASFSDPLVECAEN
                     SHRFRLDELPGTESLTETDKRDPGIVDRLNLKCPTDGSKLGPPRRFNLMFKTFMGPVE
                     DDAAVIYMRPETAQGSYVNFKNVQQSTRKKLPFGIAQIGKSFRNEISPGNFVFRMREF
                     EQMEMQYFVPPAEAARIFEDWLPRRWAWYTAYGVTEERLRFREHAPDELAHYAKKAID
                     VEYRFPFGWKELEGIHNRGDWDLSRHQKESGENLEYFDPATNESFIPWIVETAGGPDR
                     AAFTFLIDAYREEEVRGETRVSLALHPQLAPYKVAVLPLLKKRPEIVELCQKLVGDLR
                     RDMMAVYDDTASIGKLYRRQDEIGTPWCVTVDVDSLEDGAVTVRERDSMTQERVPVEG
                     VKRLILDRLAASLPQ"
     gene            complement(3268..3825)
                     /locus_tag="VIF08_08410"
     CDS             complement(3268..3825)
                     /locus_tag="VIF08_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136045.1"
                     /translation="MLRNRFVAALGLALVMGAAVAAPAAAETVIDPCSGNCGYWEVYD
                     THPGPKGAVCKYETGSYDLDWIQVRPPLMHGPFPGNTKVQWKFNILRSTNFGGSYTGI
                     YNSSWQTAMANDAIPARAGNGFSWRTWYASDPNPTGWFKVRLLLWWKNAAGSVIGKMK
                     IEYDHYQGKWNGNNNHRSEYCLQDW"
     gene            complement(3888..4706)
                     /gene="recO"
                     /locus_tag="VIF08_08415"
     CDS             complement(3888..4706)
                     /gene="recO"
                     /locus_tag="VIF08_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY8136046.1"
                     /translation="MPVPRIYHTEAIVLSRFELGEADRVLILLTPHDGKLKAIAKGVR
                     RQRSRLGGAVEPFAELYLVLARARTFDVITSASVAHAWLSLRERLESTATAWYLAELA
                     ERAVEERAFAYPVYALLKRAYQLLDDGMAPGRVARWFEYGLADALGMRPEVERCVECD
                     RVLEPDEEFRWVPILGGTLCANHPTPPAEVAPLSLAALKLLRAYRRLDIEAIAGLRLP
                     VTVEAEVEVALRRFIRAVLEREPRSLAFLDEVRARRRQVAVTMDVPAREVLPTG"
     gene            4737..5762
                     /locus_tag="VIF08_08420"
     CDS             4737..5762
                     /locus_tag="VIF08_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8136047.1"
                     /translation="MLTSSDLDRLIADTETEILGVIRMAQDDRTGALYEMVRYHLGLD
                     DDSAAHTRGKRLRPLLGLLAYESLTGDYRAALPGAAAVELGHNFSLVHDDIEDRDVER
                     RHRKTLWTVYGIAQAINAGDTLFTLSRMALHRLTELGFSDSKVLALMRLYDETCLALC
                     EGQFMDIWGAEHDEHLSVDFYFDMIGRKTAALIAASVQAGAMLATDDSGVIDAYRGFG
                     WSLGIAFQLNDDLLGIWGDEELTGKEPSDLAKHKKTLPVIYALEHAPLAEQGRLREIV
                     AMTNADGAAIQEARGIIERCGAREYTRERAHGERDAALERLASAGVVGHEAMERLRMI
                     VISAISA"
     gene            complement(5844..7157)
                     /locus_tag="VIF08_08425"
     CDS             complement(5844..7157)
                     /locus_tag="VIF08_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8136048.1"
                     /translation="MDLVIIALLILLNGLFVAAEFSLVRVRRTRIEQLVDEGSRGARR
                     VERLVSQPGRFLAVIQIGVTFVGFLSAAFAGVSIVGDLSRTLATVPVLASQAELIALL
                     VVTTLVSLFTIVFGELVPKAIALAHAERLALIFAVPIDVIGRLLNPLVWLLTSLTSAI
                     TRVLGISESTQDRVTAEELMILIERGSEQGTIEAEEQQMIGAVLELGSQRVHEVMVPR
                     IDISALPVTATLDEMIATIVNDGHSRIPIYDESIDNVIGILYAKDLLPLLKGGREPNL
                     RKIVRPPVFVPESITVDDLLHQLQRQKVHIAIVLDEYGGTAGLVTIEDLIEEIVGEIQ
                     DEYDVEEPMIEPLDDHRARVDGRASIDDLTTHFGMELDEEDREQYDTVGGLVYHEIGG
                     VPNVGDSVEVNGLTLTVESTDGRRVGKVLVVRRPAEEPADVAETG"
     gene            complement(7161..8591)
                     /gene="glmU"
                     /locus_tag="VIF08_08430"
     CDS             complement(7161..8591)
                     /gene="glmU"
                     /locus_tag="VIF08_08430"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY8136049.1"
                     /translation="MSPKLPKSAAGGSPTAATGRASHAVAVVLAAGLGTRMKSRLPKV
                     LHPVCGRPMLAYVIDAARAATGQKPLVVYSPRTEALREAFATKARFALQKKPQGTGDA
                     LAAAVAALPKEAREIVVLSGDAPLIEAASVTVMLAARRRAKSAMALAVVEMDDPTGYG
                     RIQGGPGGTVRRIIEEKDASDRQRAGNLVNAGLYAFDVAWLRKALPRLTRSATTGELY
                     LTQLVELAGADGKPAVAVEQPADSDWESELVGINDRADLAEVQLFLQYSILQRLMEAG
                     VTLHDPGSVTIEATVKIAQDVTIEPNVILRGATKIGRDTVIKSGSQLIDARIGERCQV
                     WASVIEHSEVGNDVRIGPFAHLRGGCVVGDGAEIGNFAEQKNTRFGAGSKQHHFSYLG
                     DAEVGTKVNIGAGTITANYDGERKHTTVIGDGAFIGSDTILRAPLTVGKGAYTGAGSV
                     VTRDVPPGKLAVGVPARIRERVARKG"
     gene            8755..10536
                     /locus_tag="VIF08_08435"
     CDS             8755..10536
                     /locus_tag="VIF08_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136050.1"
                     /translation="MSRVSRSDALRWLAFALALVLSLTVPNPTSQSVLGRASRQIDSA
                     LAIDSDGHAHFAYIGGDSLYYATNAAGSWVTRPIATTGYEWSPRIAIDVSGYVHIVFS
                     HRVDTEYEGIYHATNASGSWSVEYLIQDNLVQNGVDMKMTADGTIHIAWGGSPVGYGG
                     GLFYARYAAGILTMTNVNTASRGGTSGASLAIAPNGSVWVAYESSVSGNCGIEVMTNT
                     GGNWHVAASSVGGCFGNVPTLGVESDGRVHLLFWSNHDIIDTIADGTPTGTFWQSADE
                     FAMTFSPAGVPALLVERVNSGDHSLVVVSGGAVPDEVLTDNAGTVPDEAVYPNAIAVD
                     TVGKRHVVYSDTGISPDHLIYATDATGSYVKTVLDWPVVGEPAPITTPLTAPVFYGRL
                     GTDGSVPITINWQPTKAGTVGFDIQRNTENAGFLAVASPTANTWSQSLMSGKRYQHRV
                     RSRYDGGVTSTWVPDGAVVTLARIEENATSLTFTGKWTRLAQTAASGGAVRQTKTPGS
                     AVSITTSARNVSLIATTGRKMALVNVYVNGVLIKTIDLKRAENNVTRRIVWVGQFTTV
                     AVRTIKFVAVKKGTRVRVDFDAIVALI"
     gene            complement(10533..10922)
                     /locus_tag="VIF08_08440"
     CDS             complement(10533..10922)
                     /locus_tag="VIF08_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEY8136051.1"
                     /translation="MEVSVKKVISTNGAPSAIGPYSQGIDTGSLVFVAGQIGREAQSG
                     DLAEGVEGQTEQAMKNIAAILDAAGLSWADVVKTTCFLADIADFDAFNGVYKTFVVDP
                     APARSTFAVKDLPRGARVEVEAIAARG"
     gene            complement(10907..11794)
                     /gene="ispE"
                     /locus_tag="VIF08_08445"
     CDS             complement(10907..11794)
                     /gene="ispE"
                     /locus_tag="VIF08_08445"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY8136052.1"
                     /translation="MHANPRAKINLTLSVGARGADGYHSLESLILRVGLSDELVVAFA
                     ESGADTLTVTGLPGCPVEGNLVLRALGAVRRRLSHEMPPLVAHLDKRIPMAAGLGGGS
                     SDAAAAIEAAEVMWGVGLAPQVRAEIELELGADVPFFARGGDAARVSGRGEHVERLAV
                     PAGDIGVLLVTPPFGLSTAQVFARFDELGPPRSALSVDLARALRDPASLHDANDLWPA
                     AASLEPRLAEIRDELERLTRAPWMMSGSGSTLFALHASPAEAAEAGRLLLSAESESLG
                     GAIINAVDLRGPEPQWRYP"
     gene            complement(11794..12648)
                     /gene="rsmA"
                     /locus_tag="VIF08_08450"
     CDS             complement(11794..12648)
                     /gene="rsmA"
                     /locus_tag="VIF08_08450"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY8136053.1"
                     /translation="MTTDQPRGAEQTARYLKQHGLAPRKHRGQNHLVDTQVLDDIVAL
                     ADPAPRRHVLEIGPGLGVLTAAILERGAQVTAVEVDGRLATHLRNRFEREIGAGSLRL
                     VEGDALDIPIADVADPPYDLVANLPYSITSPTMHHVLGGEPRPELFVVMLQREVAERI
                     AAKPGDMSYLSVFVQYHAAVRVARIVPASAFEPAPEVSSAVLVGDVQPRRLSEEAEEQ
                     LWRLVQAGFRERRKMLHNVLPRQLPGVGRERFETALREVGIATNRRPQTVSVDEWLAL
                     AAVLGPLD"
     gene            complement(12714..13025)
                     /locus_tag="VIF08_08455"
     CDS             complement(12714..13025)
                     /locus_tag="VIF08_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136054.1"
                     /translation="MAIDLTRACPPAPGCPKYEPTEISAASGSIVLYLDNTSGAPHNF
                     LLGHQIGAPIASSPKVYGSRAITFTIENVPAGQYTFWCSVDNGYGPHYERGMVGTLVV
                     N"
     gene            complement(13327..13833)
                     /locus_tag="VIF08_08460"
     CDS             complement(13327..13833)
                     /locus_tag="VIF08_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8136055.1"
                     /translation="MSATLTHSAPVAQQHHERLMSHVDRMPALADLMDRGTAEELRAA
                     LYEMIDFMAEVLMPHMEAAERVLYPELERMMQNRHSMTPTRREHAEIRAGLADLARLR
                     TRVASPSISLKDQVELRRTIFRLYGLLKVHLAEELLYADIVEKGASPQAEAALAAAME
                     HTGTARFG"
     gene            complement(13932..15017)
                     /gene="tsaD"
                     /locus_tag="VIF08_08465"
     CDS             complement(13932..15017)
                     /gene="tsaD"
                     /locus_tag="VIF08_08465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY8136056.1"
                     /translation="MSGGSGLILAVESSCDETAVAVVRDGRRILTNVVASQVALHSAT
                     GGIVPEVAARAHLRWMIPVLEQARRDSGISDWRELEAIAVTEGPGLAGSLLVGITMAK
                     TLAWLHDLPLVPVNHLEGHIYAAWLLDPGEDEKPVPEFPVVALVVSGGHTFLVEMTDH
                     LRYRLLGQTVDDAAGEAFDKVGRLLGLPYPGGPAIMKAAAGAVNRGRVFPRAWLGETF
                     DFSFSGLKTAARREVARELGIDVAQMGSDGQDLPDSAVSELAYGFQDSVVDVLTKKTL
                     RAADEIDARTIVVGGGVAANSVLRSAIEMQAEAHGIRVVVPRPGLCTDNAAMIGAAGF
                     FRARAGARAGSDLAAKPSLKLAVREPN"
     gene            complement(15014..15541)
                     /gene="rimI"
                     /locus_tag="VIF08_08470"
     CDS             complement(15014..15541)
                     /gene="rimI"
                     /locus_tag="VIF08_08470"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY8136057.1"
                     /translation="MVARPPLKLRVEPMGLEDVPAVHRIEEQSFPIPWPDYAFRQELQ
                     TNRLAHYLVVRSGNETVAYGGLWLLVDEAHITTFAVLPEWRRQGIGGRLMVALMDLAL
                     RLNARVMTLEVRLSNRPARDLYAKFGFKPVGVRPRYYSDNGEDALIMTTEALTGAPVT
                     ALLLKMRGKYGEDAP"
     gene            complement(15520..16152)
                     /gene="tsaB"
                     /locus_tag="VIF08_08475"
     CDS             complement(15520..16152)
                     /gene="tsaB"
                     /locus_tag="VIF08_08475"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY8136058.1"
                     /translation="MILVLDTATRTPVVAVAATDGTVMADRRWQSRHRRGEELLGRIG
                     EALAEIGAGRRDLTGVVVGTGPGSFTGLRIGLATAKTIAYVLGIPIVGVSSTRALALA
                     AAKADASLAEVAIALPAGAADRYVHRFRITGGLPLELAEPQLTAAPPASGEIDGESVQ
                     GVATALAQLGAAALRTGETDDPATLVPAYVALPRGLAPAAAEMTWSPDLR"
     gene            complement(16149..16646)
                     /gene="tsaE"
                     /locus_tag="VIF08_08480"
     CDS             complement(16149..16646)
                     /gene="tsaE"
                     /locus_tag="VIF08_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY8136059.1"
                     /translation="MTAAELIAQLMTHSNDETRDFAAALATVARPGDRIALVGPLGAG
                     KTQFAKGFAAGLGVREIVNSPSFTLMAEYAGRLPLFHQDLYRLIGAEEALGGGMFDER
                     GLEGVTLTEWAERLPAELDPGRLTIAFDVVGEDERRISLKAANDQQRRYLEAAAAWSA
                     RQGQS"
     gene            16716..17084
                     /locus_tag="VIF08_08485"
     CDS             16716..17084
                     /locus_tag="VIF08_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136060.1"
                     /translation="MRVVRAFAALVVAMALLATPATSFAANRYVTYVACSTSKQAPAA
                     TSCLKSSAKAAFFKSRDASVTYKVCVRFPSGTKLCATGQSAPQGAKRVNTITSSMKGT
                     HKVTWFVGGSQVGAWTFQVT"
     gene            complement(17104..17394)
                     /locus_tag="VIF08_08490"
     CDS             complement(17104..17394)
                     /locus_tag="VIF08_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136061.1"
                     /translation="MSEYGSANELTFEDPVESLAREIQLYGMTGPAMMFLEASRPYRA
                     LGSQAMVFFDPVLRTIFGSGSDAMQRVLSDEGGIERLIERLEEIDEESGYDA"
     gene            complement(17417..>18545)
                     /gene="alr"
                     /locus_tag="VIF08_08495"
     CDS             complement(17417..>18545)
                     /gene="alr"
                     /locus_tag="VIF08_08495"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8136062.1"
                     /translation="GQSVELSAVVKADAYGHGLVPVGRVFENAGADRLCVAGIDEATA
                     LRAGGISLPILVLFPIPAADVARAAAERFEIVASDASTIAATLGAWRETMPPAETELS
                     VHLEVETGLARGGFKPDAVAAAARQITATPAVRLVGLWSHLARSEDPAATDAQVAAFE
                     AASQAVHDAGVELPPRHICATGGLFAGRAPLYEGVRVGLGLYGLLPDDFPVGAGQRSA
                     VAKLRPAMAVKCRPLRVETFAAGTPVSYGGLWTAPRESRIATLPIGYGDGWVRAYSPF
                     AGALVRGVKAPLVGSIAMDAVMADVTDVGDVGLDDEFVLIGAQEGREIVTNELARLRT
                     TIPWEVVTGMAYRVPRVYHAGPVLKGLRTLAGEARVGSSAG"
BASE COUNT         3019 a         6334 c         6219 g         2973 t
ORIGIN      
        1 gaccgaacgg ctccagccca gcacgacgga ggtcgagccg cggatggcat tgccaagatc
       61 ggtcgcacca tcgccgaacg tggcgcagcc gttgagaaac acgaccgcat ccgtgagccc
      121 gcctgggtat tggtcgcgga agaaatcggc gccgagaaga ctgaagaacc ttggcctctg
      181 cggcgcggcg ccagccccgg cgaccacctc cagctccagg ccccgaacag cgctcggcca
      241 gatgttcgcc acccctgtga gcagtgcatt ggcggcgacg acagcacggc attttccgtc
      301 gttgcacagg cggaatccgt ggctcacgat gtggaccacc tgcaatccct gccagccgcg
      361 aaagtcgtcg acggtcacat cgctgtccgc tacgttcgtg ttctcggcgt actcgacgcc
      421 gttggcgtaa tcgggcagct gcgccaggcg ctcagcgaca gcctgcccgt cgtccgaccc
      481 gccgaagtcc caccgaaacg gcgataggac gcgagctcgg cgcagggatt gacccgcgcc
      541 cgcaactatc tgctctctcc ccgcaggtgg cgacgtggcg aacgttccgg cgccgaatgc
      601 tgctttggag cgcaacgcca ccgcctgcga cgtcacaatt ggaggaggca cgatccagac
      661 gccgcgaccg ccggcgggac gaaagcggat tgcatcggcg tcgccttcga cctcggcgac
      721 gctggcctgt gcgcgcaacc aggtaaccac agcgtcgttc gtcgccccct cattgatccg
      781 gctggcggct tccgtgacga gccgctcggt ctcggcgcga acgtcatcgg ccacgtccgc
      841 gaacgagcat ggatagcttg ccgattcgca ttcaagaggc gtcggtgggc tggaaggcga
      901 gggtgtggat gccccaccac tgcacgccgt ggccgcaacg aacaggatcg ccaacggcaa
      961 gcgcgtcagt gatccaccct tccagggcac gcggcctccg aaactggccg gtggccggca
     1021 cggcgatccg gcctgagcgg gccagactat tcgtgtcgct cgctcatttc aatccggagc
     1081 gccggctagg ccggctcctc gcgtcagtcg atcgggaagg tcaggaagcg ctctgtgccc
     1141 ggctcgtcca agaactggat gtcggcatcg tccagcgata ccgtgaattc gtcggtcagc
     1201 ggctggccgg tgatcacgcg gtcagcgacc agcttgaagg tgtaatcgcc ggcggtgaag
     1261 aaccagccct gcggcgcctc gaatagggcg agcggcgccg ccgcgtcgcg tgggctgacc
     1321 agcaacggca cggcgtcacc gacgtattcg tagctcagcc cgccgccagg ttgactcacc
     1381 aagcgcagct gctgcgtcca ctcgaggtcc gtgggctctc ccgggcctga cacctcgagt
     1441 cgcatgtcgc ggatgacctc gatccgctcg ctccggccgg tgctcacgaa ggccggctgc
     1501 agatagaggt aggccgcacc cgactgatga ccctgggcga cccggacctg gtcgggcagg
     1561 atcacgagca cttcgggctg ctgcagcgag atcaggatcg cggccgccga tagcggcaat
     1621 gacagcagcg cagccgccag ggggacccag cgcagcgtga ggacgctgcg cgccacgcga
     1681 cgccacccac cttgcgcgtc atcgccgtca tcacgggtct gaggttcgcg tccggccgac
     1741 cggtcggtca ttgcggcagg ctcgccgcca ggcgatcgag gatgagccgc ttcacgccct
     1801 cgaccggcac gcgctcctgg gtcatcgagt cgcgctcacg caccgtgacc gcgccgtcct
     1861 cgagcgaatc gacgtcgacc gtgacgcacc acggcgtgcc gatctcgtcc tggcggcgat
     1921 agagcttgcc tatcgacgcg gtgtcgtcat agaccgccat catgtcgcgc cgcaggtcgc
     1981 ccacgagctt ctgacacagc tcgacgatct cgggccgttt cttgagcagc ggcagcacgg
     2041 ccaccttgta cggcgcgagc tgcgggtgga gcgccagcga gacgcgcgtc tcgccgcgca
     2101 cctcctcctc gcggtaggcg tcgatcagga atgtgaacgc ggcgcggtcc gggccgccgg
     2161 ccgtttcgac gatccacggg atgaagctct cgttcgtggc tggatcgaag tactcgaggt
     2221 tctcgccgct ctccttctgg tgacgcgaca ggtcccaatc accgcggttg tggatgccct
     2281 cgagctcctt ccagccgaac gggaagcggt actcgacgtc gatcgccttc ttggcgtagt
     2341 gcgccagctc gtccggcgcg tgttcgcgga acctgaggcg ctcctcggtg acgccgtacg
     2401 cggtgtacca ggcccagcgc cggggcagcc aatcttcgaa gatgcgcgct gcctccgccg
     2461 gtgggacgaa gtactgcatc tccatctgct cgaactcgcg cattcggaac acgaagttgc
     2521 cgggcgagat ctcgttgcgg aagctcttgc cgatctgggc gatgccgaac ggcagcttct
     2581 tgcgcgtcga ctgctggacg ttcttgaagt tgacgtagct gccctgcgcc gtctccgggc
     2641 gcatgtagat caccgccgcg tcgtcctcga ccgggcccat gaacgtcttg aacatgaggt
     2701 tgaagcggcg cggcgggcca agcttcgatc cgtccgtggg gcacttcagg ttcaggcgat
     2761 cgacgatgcc ggggtcgcgc ttgtccgtct cggtcaggct ctccgtgccc ggcagctcat
     2821 cgagccggaa gcggtggctg ttctccgcgc actcgaccaa tgggtcgctg aaggacgcga
     2881 catggccgct cgtcacccag acctggggat gcatcaggat gccggcatcg agcccgacga
     2941 tgtcgtcgcg ctcctggacc atcgcccgcc accacgcgcg cttgacgttg ttcttcaact
     3001 ccacgccgag cggcccgtag tcccacacgg cgttgatgcc gccatagatc tcgctcgagg
     3061 ggaagacgaa gccgcgccgc ttggcgagcg acacgatcgt gtcgaggttg gcgacggcgg
     3121 gcgtcggatc cgggttggcg gtcacagggt ctcctctccg cgccgggcgc atggcatggc
     3181 cggtcgcgga cggcgattct cgcatgaaag aaggcccggc cgtttccggc cgggccacag
     3241 atcactggtt agataagccg gtgcgggcta ccaatcctga aggcagtact cggaccggtg
     3301 gttgttgttg ccgttccact tgccctggta gtggtcgtac tcgatcttca tcttgccgat
     3361 cacgctgccg gcggcgttct tccaccacag caagaggcgt accttgaacc agccggtcgg
     3421 attcgggtcg gaggcgtacc aggttcgcca gctgaagccg ttgccagcgc gggccggtat
     3481 ggcgtcgttg gccattgcgg tctgccagga gctgttgtag atgccggtgt aggacccacc
     3541 gaagttggtc gagcggagga tgttgaactt ccactggacc ttggtgttac ccggaaacgg
     3601 cccgtgcatc agcggtggcc gcacctggat ccaatcgagg tcatagctac ctgtctcgta
     3661 cttgcacacc gcaccctttg gacccggatg ggtgtcgtaa acctcccagt agccgcagtt
     3721 gccggagcac gggtcgatca ccgtctcggc ggccgcgggc gcggcgacgg cagcgcccat
     3781 tacgagagcc agccccaacg cggcgacgaa tcggttacgc agcatcgaat tcctcccttt
     3841 gaattgcctg aacccacacg gctaggacgc caatgatgac ctagtggcta gccggtggga
     3901 aggacctcgc gcgcgggcac gtccatcgtc accgccacct gccggcggcg cgcgcggacc
     3961 tcgtcaagga acgccagcga acgtggctcg cgctccagca cggcgcgaat gaaccgtcgc
     4021 agcgcaacct cgacttcagc ttccaccgtg accggcaggc gcagcccggc gatggcctcg
     4081 atgtcgagcc ggcggtaggc gcgcagcagc ttgagcgcgg ccaacgagag cggcgcgacc
     4141 tcggccggcg gggttggatg gttcgcgcac agcgtcccgc ccaggatcgg tacccagcgg
     4201 aactcctcgt cgggctcgag cacgcgatcg cactcgacgc agcgctcgac ctccggccgc
     4261 atccccagcg cgtcggcgag gccgtactcg aaccagcgcg ccacccggcc cggcgccata
     4321 ccgtcatcga gcagctggta ggcgcgtttg agcagtgcat agaccgggta ggcgaaggcg
     4381 cgctcctcga ccgcgcgctc ggccagctcg gccagatacc acgcggtggc agtcgattcg
     4441 agccgctcac gcagcgacag ccaggcgtgg gccacgctgg ccgaggtaat gacgtcgaac
     4501 gtacgggcgc gggcgagcac caggtacagc tccgcgaacg gttccacggc gccgcccagg
     4561 cgcgagcgct gccggcgcac gcccttggcg atggccttga gcttgccgtc gtgcggcgtc
     4621 agcaggatga gcacgcgatc ggcctcgccc agctcaaagc gggaaaggac gatcgcctcc
     4681 gtgtgataga tccgcggcac cggcacgcgt aaaggctacg ccctatgctc ggcccaatgc
     4741 tgacctcgtc ggacctcgat cggctcatcg ccgataccga aacggagatc ctgggcgtga
     4801 ttcgcatggc ccaggacgac cgcacgggcg ccctgtacga gatggtccgc taccacctgg
     4861 ggctcgatga cgacagcgcg gctcacaccc gcggcaagcg cctgcggccg ctgctcgggc
     4921 tgctcgccta cgagtcgctc accggcgact accgcgcggc ccttccgggt gcagcggcag
     4981 tcgagctcgg ccacaacttc tcgctcgtcc acgacgacat cgaggaccgc gacgtcgagc
     5041 ggcgccatcg caagacgctc tggacggtgt acggcatcgc ccaggcgatc aacgcgggcg
     5101 acacgctctt caccctgtcg cgcatggcgc tgcaccgcct gacggagctc ggcttctccg
     5161 actccaaggt gctggccctc atgcgcctgt acgacgagac ctgcctggcg ctgtgcgagg
     5221 gccagttcat ggacatctgg ggcgccgagc acgacgagca cctgagcgtc gacttctact
     5281 tcgacatgat cggccgcaag accgctgccc tcatcgccgc gtcggtccag gccggtgcga
     5341 tgctggccac cgatgattcg ggggtcatcg acgcctaccg cggcttcggc tggtcgctgg
     5401 gcatcgcctt tcagctcaac gacgacctgt tgggcatctg gggcgacgag gagctgaccg
     5461 gcaaggagcc gtcagacctc gccaagcaca agaagacgct gccggtcatc tatgccctcg
     5521 aacacgcgcc attggccgag caggggcggc tgcgcgagat cgtggcgatg accaatgccg
     5581 acggcgccgc gatccaggag gcgcgcggca tcatcgagcg ctgcggcgca cgcgagtaca
     5641 cgcgcgagcg cgcgcacggt gagcgcgacg cggccctcga acgcctcgcg tcagcgggtg
     5701 tcgtcggtca cgaagcgatg gagcgcctgc gcatgatcgt gatctccgcg atcagcgcct
     5761 gacggtcgct gccgaatccg aacccgtgat gaatctggtc gattcgaagt agccgctagg
     5821 cggcgtttcc ggtgcgcgcg ccgctagccg gtctccgcga cgtcagctgg ctcttccgca
     5881 ggtctgcgca ccaccagcac cttgccgacc cgccggccgt cggtcgactc gacggtgagc
     5941 gtcagcccgt tcacctcgac ggagtcacct acgttcggca cgccgcctat ctcgtggtag
     6001 accagcccgc caacggtgtc gtactgctcg cggtcctcct cgtcgagctc catcccgaaa
     6061 tgcgtcgtca ggtcgtcgat cgacgcgcgg ccatcgaccc gcgcccggtg atcgtcgagc
     6121 ggctcgatca tgggctcctc gacgtcgtac tcgtcctgaa tctcgccgac gatctcctcg
     6181 atcaggtcct cgatcgtgac caggccggct gtgccgccgt actcgtcgag cacgatcgcg
     6241 atgtgcacct tctggcgctg cagctggtgg agcaggtcgt cgaccgtgat cgactcgggc
     6301 acgaacaccg gcggacgcac gatcttgcgc aggttcggct cccggccgcc cttgagcagt
     6361 ggcagcaggt ccttggcgta gaggatgccg atgacgttgt cgattgactc gtcgtagatc
     6421 gggatgcgcg agtggccgtc attcacgatc gtggcgatca tctcgtcgag cgtcgcggtt
     6481 acggggagcg ccgagatgtc gatacgcggc accatcacct cgtgcacgcg ctggctgcca
     6541 agctcgagca ccgcaccgat catctgctgc tcctcagcct cgatggtgcc ctgctccgag
     6601 ccgcgttcga tcaggatcat cagctcctcg gccgtgacgc ggtcctgggt cgactcggag
     6661 atgcccagca ctcgcgtaat ggcgctggtc aggctcgtca gcagccacac gagtgggttc
     6721 agcaaccgcc cgatgacgtc aatcgggacg gcgaagataa gcgccagccg ctcggcatgg
     6781 gcgagcgcga tggccttggg caccagctca ccgaaaacga tggtgaacag gctgaccagg
     6841 gtggtgacga caaggagcgc tatcagctct gcctgcgagg ccaggacggg tacggtggcc
     6901 agggtgcggg acaggtcgcc gacgatgctg acgcccgcga aggcggcgga caagaagccc
     6961 acgaacgtca cgccgatctg gatcacggcc aggaagcggc ccggctgtga cacgagtcgc
     7021 tcgacccggc gcgcgccgcg gctgccctcg tcgaccagct gctcgatgcg tgttcggcgc
     7081 acacgcacga gcgagaactc ggcggccacg aacaggccgt tcaacaggat gagcagcgcg
     7141 atgattacga ggtccattga tcagcccttg cgggccacgc gctccctgat gcgcgccggc
     7201 acgcccaccg ccagcttgcc cggcggcacg tccctggtga cgacggaccc ggcgccggtg
     7261 taggcgccct tgcccacggt cagcggcgcg cgcaggatcg tgtcgctgcc gatgaacgcg
     7321 ccgtcgccga tgacggtcgt gtgcttgcgc tccccgtcgt agttggcggt gatcgtccca
     7381 gcgccgatgt tcaccttcgt gccaacttcc gcatccccca gatacgagaa gtggtgttgc
     7441 ttggagccgg cgccgaagcg cgtgttcttc tgctcggcga agttgccgat ctcggcgccg
     7501 tcgccgacca cgcaaccacc gcgcaggtgg gcgaaggggc cgatcctgac gtcgttgccg
     7561 acctccgagt gctcgataac gcttgcccac acttggcagc gctcgccgat ccgggcgtcg
     7621 atgagctggc tgcccgactt gatcaccgtg tcgcggccga tcttcgtcgc gccgcgcagg
     7681 atcacgttgg gctcgatggt cacgtcctgg gcgatcttca cggtggcctc gatcgtcacc
     7741 gacccgggat cgtggagcgt cacgcccgcc tccataaggc gctgcaggat ggagtactgg
     7801 aggaagagct ggacctcggc cagatcggcc ctgtcattga tcccgacgag ctccgattcc
     7861 cagtccgaat cggccggctg ctcgaccgcg acggccggct tgccatccgc acccgccagc
     7921 tcgaccagct gggtgagata cagctcgccc gtcgtcgccg acctggtcag ccgtgggaga
     7981 gccttgcgca gccacgccac gtcgaacgcg tagaggccgg cgttgaccag gttgccggcg
     8041 cgctgcctgt cgctggcgtc cttctcctcg atgatgcgcc tcacggtgcc gccgggtccg
     8101 ccctggatgc ggccatagcc tgtcgggtcg tccatctcga cgaccgccag tgccatcgcg
     8161 gatttcgcac gtcgtcgcgc cgcgagcatc acggtcacgg acgctgcctc gatcagcggc
     8221 gcgtcaccgc tgagcacgac gatctcccgc gcctccttgg gcagggcggc gaccgcggcc
     8281 gcgagcgcgt cgccagtgcc ctgcggcttc ttctggagcg cgaacctggc cttggtggcg
     8341 aaggcctcgc gcagtgcctc ggtgcggggc gagtagacga cgagcggctt ctggccggtc
     8401 gccgcccggg ccgcgtcaat cacgtaggca agcatgggcc tgccgcagac cgggtggagc
     8461 accttgggca ggcgcgactt catgcgcgtg ccgaggccgg cagcaagaac tacggcgacg
     8521 gcgtgagagg cccgacccgt cgcggcggta gggctaccgc cggcggcgga tttgggcagt
     8581 ttgggcgaca tctggagtcc cgtaacccta gggccggtcc aattcgcggt caaacgagcc
     8641 cgaccggcgc gatgtgacct ctggcatatt caatttggct cgcgggatgc gacgatatgc
     8701 gccagtcgct cgctgctgag gggcgcgggc cggccagaag gagcttagag agcgttgtct
     8761 cgggtatctc gatccgatgc gttgaggtgg ctcgcgttcg cgcttgccct cgtgctatcg
     8821 ctgaccgtcc caaatccaac cagccaatca gtgctcgggc gcgcgtccag gcagattgat
     8881 agcgccctgg cgatcgattc agacggccac gctcacttcg cctacatcgg cggcgacagt
     8941 ctctactacg caacgaatgc cgccggatcg tgggtcacgc gcccgatcgc gacgacaggc
     9001 tacgaatggt caccgcgtat cgccatcgat gtctcgggct atgtacacat cgtcttttcg
     9061 catcgtgtcg acaccgagta cgagggcatt tatcacgcca cgaacgccag cggatcgtgg
     9121 tccgtcgagt acctgataca ggacaacttg gttcagaacg gcgtggacat gaagatgact
     9181 gccgacggga cgatccacat cgcctggggc gggtcgccgg tcggttacgg aggaggcttg
     9241 ttctacgcca ggtacgcggc cggcatcctg acaatgacca atgtgaacac ggcgagccgt
     9301 ggtgggacaa gcggagcgag tctggccatt gcgccgaatg gctccgtctg ggtcgcatac
     9361 gagagcagcg tgagcggcaa ctgcggcatc gaggtcatga cgaacaccgg cggcaattgg
     9421 cacgtggccg cctccagcgt cggcgggtgc ttcgggaatg tgccaacact tggcgtcgaa
     9481 tccgacggga gggtccacct cctgttttgg tccaatcacg acatcatcga taccatcgcg
     9541 gatgggactc caacgggcac cttctggcaa tctgccgacg agttcgcaat gaccttctct
     9601 ccggcgggcg ttcctgcact gctggtcgaa agagtgaatt cgggtgatca ctccctggtc
     9661 gtcgtgagcg gcggagcggt ccccgacgag gtcctaacag acaacgccgg caccgtcccc
     9721 gacgaagccg tttatcccaa cgcgatcgcg gtcgacacgg tgggtaagcg tcatgtcgta
     9781 tacagcgata cagggatttc gccagaccat ctcatctacg cgactgacgc gaccggttcg
     9841 tacgtgaaga cggtattgga ctggccggta gtcggtgagc cggcgccgat caccacgcca
     9901 ctgactgcgc cagtgttcta tggacgcctg ggcaccgacg ggtcggtgcc catcaccatc
     9961 aactggcagc caactaaggc tggcaccgtc ggcttcgaca tccaacgaaa caccgagaac
    10021 gctggctttc tggccgtcgc gtcgcccacc gccaacacct ggtcgcagtc cctgatgagt
    10081 ggcaagcgct accagcacag ggtccgatcg cgctacgacg gcggggtgac cagcacctgg
    10141 gtgcccgacg gtgcggtagt taccttggca cgaatcgagg agaacgcgac aagcctcacg
    10201 ttcaccggaa aatggacgcg cctcgcgcag accgcggcgt cgggcggcgc cgtgcggcaa
    10261 acgaagactc cggggtccgc cgtcagcatc acgacgtctg cgcgcaatgt ttcgcttatc
    10321 gcgacaaccg gccgcaagat ggcgctcgtc aacgtctacg tgaacggcgt cctcatcaag
    10381 acgatcgatt tgaagcgggc cgagaacaac gtcacccgcc gaatcgtctg ggtcggccag
    10441 ttcaccacgg tcgcggttcg gaccatcaag ttcgtggccg tgaagaaggg cactcgcgtc
    10501 cgcgtggatt tcgacgcgat cgtggccctg atctaacccc gagcggcgat cgcctcgact
    10561 tcgacccgcg cgccgcgcgg caggtccttg accgcgaacg ttgaacgcgc gggggcggga
    10621 tcgaccacga aagtcttgta gacgccgttg aacgcgtcga aatcggcgat gtccgccaag
    10681 aagcaggtcg tcttcaccac gtcggcccac gacaggcccg cggcgtcgag gatcgcggcg
    10741 atgttcttca tcgcctgctc ggtctgtccc tcgactccct cagccaggtc gccgctctgc
    10801 gcctcgcgcc cgatctggcc cgcgacgaac acgagcgacc ccgtatcgat gccctggctg
    10861 taggggccga tcgcgctcgg cgcgccattg gtgctgatga ccttcttcac ggatacctcc
    10921 actgtggctc tggtcccctc agatctacgg cgttgatgat cgcgcctccg agagattccg
    10981 attcggccga caacaggagc ctgccggctt cagccgcttc cgccggcgac gcatggagcg
    11041 cgaatagggt cgagccgctg ccgctcatca tccacggggc acgggtgagg cgctcaagct
    11101 cgtcccgaat ctcggcgagg cgcggttcga gcgacgccgc cgcgggccac aggtcgttcg
    11161 cgtcgtggag cgacgccgga tcgcgtaacg cccgcgcgag gtcgacggat agcgcactcc
    11221 gcggtgggcc gagctcgtcg aagcgcgcga agacctgcgc ggtgctgagc ccgaagggcg
    11281 gcgtcacgag caggacgcca atatcgcccg ccggcacagc cagacgctcc acgtgctcgc
    11341 cgcggccgct gacccgcgcc gcgtcgccac ctcgggcgaa gaacgggacg tcggcgccca
    11401 gctcgagctc gatctcggcg cgcacctgcg gcgcgaggcc aacgccccac atgacctccg
    11461 ccgcctcgat ggccgcggcc gcatccgacg agccgccgcc cagcccggcc gccataggga
    11521 tgcgcttgtc gagatgagcg acgagcggcg gcatctcgtg gcttaggcgg cggcgcacgg
    11581 cgccaagtgc acgcaacacg aggttgcctt cgaccgggca gcccggcagc ccggtgaccg
    11641 tgagcgtgtc cgcgccactc tctgcaaacg ccacgaccag ctcgtcggag agtccgacgc
    11701 ggaggatcag cgactcgagc gagtgatagc catctgcgcc gcgcgcccca acggacagcg
    11761 tcaggttgat cttggcgcgc ggattggcat gcatcagtcg agcggcccca ggacggccgc
    11821 gagcgcgagc cactcatcaa cagatacggt ctgcgggcgg cgattcgtcg cgatgcccac
    11881 ttctcgcagc gcggtctcga agcgctcgcg gccaacgccc ggcaactggc gcggcagcac
    11941 gttgtggagc atcttgcgcc gctcgcggaa gcccgcctgg acgagccgcc acagctgctc
    12001 ctccgcttcc tcggacaggc gccgcggctg gacgtcgccc acgagcacgg ccgacgaaac
    12061 ctccggcgcg ggctcgaatg cgctggccgg cacgatacgc gcgacgcgca ctgcggcgtg
    12121 gtactggacg aagacgctca ggtagctcat gtcccccggc ttggcggcga tccgctcggc
    12181 gacctcgcgc tgcagcatca cgacgaaaag ctctggtcgc ggctcgcccc ccagcacatg
    12241 gtgcatcgtc gggctggtga tgctgtaggg caggttggcg accaggtcgt agggtggatc
    12301 agccacgtcg gcgatcggta tgtccagggc gtcgccttcg accagccgaa gagagccggc
    12361 accgatctcc cgctcgaacc gattccgaag atgggtggcc agccggccat cgacctccac
    12421 cgcggtgacc tgcgcgccgc gctcgaggat tgccgccgtc agcacgccca gcccggggcc
    12481 gatctcgagg acgtgcctcc tgggcgccgg atcggccaac gccacgatgt cgtcgagcac
    12541 ctgcgtgtcg accagatggt tctggccgcg gtgcttgcgc ggcgcgaggc cgtgctgctt
    12601 cagatatcgc gccgtttgct cggcaccgcg cggttgatcg gtcgtcacct tggccaaagg
    12661 ctaacgccag ggcatcgcgc gacgtggcaa gacgatccgc gccgcgcccg accctagttg
    12721 acgaccagcg tccccaccat gccgcgttcg tagtgcggcc catagccgtt gtccacgctg
    12781 caccagaacg tgtactggcc tgcgggcacg ttctcgatcg tgaacgtgat ggcccggctc
    12841 ccatacacct tgggagatga ggcgatcggc gcgccgatct ggtgtccgag caagaagttg
    12901 tgcggcgcac cggaggtgtt gtccaggtag agaacgatcg atccggaagc cgcgctgatt
    12961 tctgtcggct cgtatttcgg gcatccaggc gcgggcggac aggccctggt cagatcgatg
    13021 gccatctcga ccgttatcga gccctcgggg gcggccgtcg aggaaacgga tgaaacggag
    13081 agggtcggca gcggaaccgt gggcgaaggg cctggcgttg ccggcagcgg cgttggcgtc
    13141 gcgggtgagc caggcgtggc cgttatgacg gggctcgcag tcggctgagc ggcgctggga
    13201 gtgggactcg ccggagccga cgagcaggcc gtgacgacca gggcggcggc gatgaatccg
    13261 gcccacgtac ggttgggcgc taacccaggc accgcggcag tatagccagc gccgcgcgcc
    13321 aatctgtcag ccgaaacgag cggtcccggt gtgctccatg gccgctgcca gggccgcttc
    13381 ggcctgaggg gaagcgccct tctcgacgat gtctgcgtac agcaactcct ctgccaggtg
    13441 gaccttgagc aggccgtaca ggcggaagat cgttcgacgc agttcaacct gatccttgag
    13501 cgagatagag ggcgaagcga ccctggtgcg cagccgagcg aggtcagcca ggccggcacg
    13561 gatttcggcg tgctcgcgtc gggtgggggt catcgagtgg cggttctgca tcattcgctc
    13621 gagctcggga tagagcacgc gctcggcggc ctccatgtgc ggcatgagca cctcggccat
    13681 gaagtcgatc atctcataga gtgcggcgcg gagttcctcg gccgtgccgc gatccatcag
    13741 gtcggccagc gccggcatgc ggtcgacatg gctcatcagt cgctcatggt gttgctgcgc
    13801 gactggcgcg gagtgggtga gcgtggcgga catgggctcc tcctctggtc tggctggtat
    13861 aggccacagc cgcggctcat ccacgggcca cacgtcacat tgccgcgggc cgacgtaacc
    13921 gcctggtccg gctagttggg ctccctcacg gccagcttga gcgatggctt ggcggcgagg
    13981 tcggagccgg cacgggcacc ggcgcgggcg cggaagaagc ctgcggcacc gatcatcgcc
    14041 gcgttgtcgg tgcacagtcc cggccgcggc acgacgacgc ggatgccgtg ggcctcggct
    14101 tgcatctcga tggcgctgcg caacacggag ttcgcggcca ctcccccgcc gaccacgatc
    14161 gtccgcgcgt cgatctcatc ggccgcgcgc agggtcttct tcgtgagcac gtcgacgacc
    14221 gagtcctgga agccgtaggc cagctcactg acggccgaat cgggcaggtc ctggccgtcg
    14281 gatcccatct gtgcgacatc gatgcccagc tccctggcga cctcgcgccg cgcggcggtc
    14341 ttgaggccgc tgaacgagaa gtcgaacgtc tcgcccagcc aggcccgggg aaacacgcgg
    14401 ccccgattga cagcgcctgc agccgccttc atgatcgcgg gcccgcccgg gtagggcagc
    14461 ccgagcagcc ggccgacctt gtcgaacgcc tcgcccgcgg cgtcgtcgac cgtctgcccg
    14521 agcaggcggt agcgcaggtg gtccgtcatc tcgaccagga acgtgtggcc gccgctgacg
    14581 acgagcgcca cgaccgggaa ctccggcacc ggcttctcat cctcgcccgg gtccagcaac
    14641 cacgcggcgt agatgtgacc ctcgaggtgg ttgaccggga cgagcggcag atcgtgcagc
    14701 caggccaggg tcttggccat ggtgatcccg accagcagcg agccggccag ccccgggccc
    14761 tcagtgacgg cgatggcctc gagctcgcgc caatcgctga tgcccgagtc gcgtcgcgcc
    14821 tgctcgagca ccgggatcat ccaacgcagg tgcgcccgtg ctgcgacctc gggcacgatg
    14881 ccgccggtcg cgctgtgcag cgcaacctgg ctggcgacga cgttggtcag gatgcgccgc
    14941 ccgtcgcgca cgaccgcgac cgcggtctcg tcgcacgacg actcgaccgc gaggatgagg
    15001 ccgctccccc cgctcatggc gcgtcctcgc cgtacttgcc gcgcatcttc aaaagcagcg
    15061 ccgtcacagg cgcgccagtc agtgcctcag tggtcatgat cagcgcgtcc tcgccgttgt
    15121 cggagtagta gcgcgggcgc acaccgaccg gcttgaagcc gaacttcgcg tacaggtcgc
    15181 gtgccgggcg gttggagagg cgcacctcga gggtcatgac ccgcgcgttg agccggagcg
    15241 cgagatccat caacgcgacc atcaggcggc cgccgatgcc ctgccggcgc cactcgggca
    15301 ggaccgcgaa ggtcgtgatg tgcgcttcgt cgacgagcag ccacaggccg ccataggcga
    15361 ccgtctcgtt gccactgcgg accacgagat agtgcgccag gcggttggtc tgcagctctt
    15421 ggcgaaaggc gtagtcgggc catgggatgg ggaagctctg ttcctcaatg cggtgcactg
    15481 cgggcacgtc ctcgagcccc atcggctcga cgcgcagctt cagcggaggt cgggcgacca
    15541 tgtcatctcc gccgcggcgg gggcgaggcc gcgcggcagg gccacgtagg cgggcacgag
    15601 cgttgccgga tcgtccgtct cgcccgtcct gagggcggcc gcgcctagct gagccagcgc
    15661 ggtggcgacg ccttggacgc tctcgccgtc gatctcaccg ctcgcgggtg gcgccgcagt
    15721 gagttgcggc tcggccagct ccaagggcag cccgccggtg attcggaagc ggtgcacata
    15781 tcgatccgcg gcgcccgccg gcagcgcgat cgcgacctcg gccagggacg cgtccgcctt
    15841 cgcagctgcc agcgcaagcg cgcgcgtgct ggacacaccc acgatcggga tgcccagcac
    15901 ataggcgatc gtcttggccg tggccagtcc gatgcgcagg ccggtgaatg agcccgggcc
    15961 ggtcccgacc accacgccgg tcaggtcgcg tcggccggcg ccgatctcgg ccagggcctc
    16021 ccctatccgg ccgagcagct cctcgccgcg gcgatggcgg ctctgccagc gccggtcggc
    16081 catgaccgtg ccgtccgtgg ccgccacggc gacgaccggc gtccgcgtcg cggtgtcgag
    16141 gacaaggatc atgactggcc ctgccgcgcg gaccaggcgg ccgcggcttc caggtagcgc
    16201 cgctgctgat cgttcgcggc cttcaggctg atacgccgct cgtcctcgcc cacgacgtcg
    16261 aacgcgatgg tcaaccggcc aggatcgagc tcggcgggga gccgctccgc ccactcggtc
    16321 agcgtcaccc cttcgaggcc gcgctcatcg aacataccgc cgcccagcgc ctcctccgcg
    16381 ccgatgagtc ggtagaggtc ctggtggaag agcggcagcc ggccggcgta ttcggccatc
    16441 aacgtgaaac tgggcgaatt gacgatctcg cgcacgccca ggccagccgc gaaacccttg
    16501 gcaaactggg tcttgccggc gccgagcggc cccacgagcg cgatgcggtc accgggacgg
    16561 gcaacagtgg ccaaggcggc cgcgaagtca cgcgtctcgt cgttggagtg agtcatcaat
    16621 tgcgctatca gttcggccgc ggtcatttcg atgaagttac cggaaagtcc gttggcgtac
    16681 gatgcacgtc acttgcacag cctgaggggg tcccgatgag agtcgtccgg gcgttcgccg
    16741 cgctggtagt ggcgatggcg ttgcttgcga ctcccgcaac cagctttgcc gccaaccggt
    16801 acgtaactta cgtcgcgtgc agcacctcga agcaggcgcc ggccgcgacc tcgtgcctga
    16861 agagcagcgc caaggccgcc ttcttcaaga gccgcgacgc gtcggttacc tacaaggtgt
    16921 gcgtgaggtt tccgagcggc acaaagctat gtgccacggg gcagtcggcg ccgcagggcg
    16981 ccaagcgcgt aaacaccatt acctcgtcga tgaagggcac ccacaaggtg acatggttcg
    17041 tgggcgggtc gcaggtcgga gcgtggacct tccaggtgac ttgagagtca gacggctgcg
    17101 ggatcaggcg tcgtagccgg attcctcgtc gatctcctcg agacgctcga taaggcgctc
    17161 gatgccgccc tcgtcggaca ggacgcgctg catggcgtcc gatccgctgc cgaagatagt
    17221 gcgcagcacc gggtcgaaga agaccatcgc ctggctgccg agcgcgcggt agggccggct
    17281 ggcctcaagg aacatcatcg ccgggcccgt catgccgtac agctggatct cgcgcgccag
    17341 cgattcgaca gggtcctcga aggtcagctc gttggccgat ccgtattcgc tcatcgatct
    17401 ccctaagaca cgcccgttac ccggcgctgg aaccgacccg ggcctcccct gccagcgtgc
    17461 gcaggccctt tagtactggg ccggcatggt acacccgggg tactcggtag gccatgcctg
    17521 taacgacttc ccagggaatc gtggtgcgca gccgcgccag ctcgtttgtc acgatctcgc
    17581 gcccttcctg ggcgccgatc agcacaaact cgtcatccaa ccccacgtct ccgacatcgg
    17641 tcacgtcggc catgacggca tccatggcaa ttgagcccac caagggggct ttgacgccgc
    17701 gcaccagggc gccggcgaat ggcgaatagg cccgtaccca gccgtcgccg tagccgatcg
    17761 gcagggtcgc gatgcgcgac tcgcgcggcg ccgtccacag gccgccgtag ctgaccggcg
    17821 tgccggccgc gaaggtttcc acgcgcagcg gccggcactt gacggccatg gccggtcgca
    17881 gcttcgccac ggccgatctc tggccggcgc ccaccgggaa gtcgtcgggc agcagtccgt
    17941 acaggcccag ccctacgcgc acgccctcgt agagtggggc gcggccggcg aacaggccgc
    18001 cggtggcgca tatgtggcgc ggcggcagct cgacgcccgc atcgtgcacg gcttgtgatg
    18061 ccgcctcgaa ggccgccacc tgggcgtccg tcgcggcggg atcctccgac cgggcgaggt
    18121 gcgaccagag accgaccagg cggacggccg gcgtggccgt gatctggcgt gcggcggcgg
    18181 ccacggcgtc cggcttgaag ccgccgcgcg ccagtccggt ctcgacctca aggtggacac
    18241 tcagctccgt ctctgccggc ggcatggtct cgcgccacgc gccgagtgtc gccgcgatag
    18301 tcgacgcgtc ggaagcgacg atctcaaagc gctcggccgc ggcacgggca acgtccgcgg
    18361 cgggtatcgg gaacagcacc aggatcggca ggctgatgcc gccagcccgc agcgcggtcg
    18421 cctcgtcgat gccggcgacg cagagacggt ccgcgccggc gttttcgaac acccgaccga
    18481 ccgggaccag gccgtggccg taggcgtcgg ccttcacgac ggcgctcagc tcgacgctct
    18541 gcccg
//
LOCUS       DATBRX010000222         9517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_430083, whole genome shotgun sequence.
ACCESSION   DATBRX010000222 DATBRX010000000
VERSION     DATBRX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430083"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VIF08_08500"
     CDS             <1..412
                     /locus_tag="VIF08_08500"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY8136063.1"
                     /translation="AQIDRHGDLNSTVIGPYETPKTRLPGSGGACEIAQNAKSIFVIM
                     RQSPRSFVERLDFRTSVGTRVRVVVTDLGVYHPVDGELQLDALHEGVTLDQVRGAMGW
                     TPRVASTLAQTPPPSDAELRMIREELDPAGVYTK"
     gene            453..1730
                     /locus_tag="VIF08_08505"
     CDS             453..1730
                     /locus_tag="VIF08_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEY8136064.1"
                     /translation="MAYRRAIALICLLITACGSPTASPTSQPTAAPGSTMTGAPTISP
                     APTAGSTPGATATLAPTDVPTRAPTDAPPPFVTPTTWVNVASMNHPRRGNDAVRLLDG
                     RVLAAGGETESTDILDSAEVYDPATDTWTDVASMGDRRVYAIVVLLADGRVLLAGGYG
                     SGGDDLLTSAELFDPATNTFSPTGPMGVARARFQGVRLDDGRVLVMGGNKQGGGALDS
                     AEVYDPIAGTWSPTGSMHTTREAGRAVKMADGRVIVTGGSDGVGDGTPTNSTEIYDPR
                     IGTFSQIGPMTIARDRHAIVLLGDSRVILAGGQSNSGVERESDIFDPRSGATVWTATA
                     PLGDRRFAASYVTMADGNGLVCGGFNGSAYLRGCEAYDSATDSWTNTEDLHSATSGAL
                     MVTLADGRVLICGGLGKGDVTLKTCELFTEVSG"
     gene            complement(1727..2326)
                     /locus_tag="VIF08_08510"
     CDS             complement(1727..2326)
                     /locus_tag="VIF08_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136065.1"
                     /translation="MTEPRNVTPDGAQPDDFHRNYYNERAQGPEWAAWGWGRRGWSFP
                     WLGVLLVLVGVGLLIQYFVPGVSSGTLVLTAIALALLAAWLFGRSRWAVVPGLLILAL
                     ALAGLARELNVYSGPGLTAGALAVAFLLIWVLDYSRGVRSTWPLWGLAIFGLIALVQA
                     SGRISGIPELGALWPVLIIVIGVLLLMSARRGPGVPGRG"
     gene            complement(2367..3110)
                     /locus_tag="VIF08_08515"
     CDS             complement(2367..3110)
                     /locus_tag="VIF08_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY8136066.1"
                     /translation="MHVTVRLFAILRQQAGWRERQIELPEKATIEDAWQALVAIAPAL
                     AGSREVVRFARNREYSAAETRLEDGDELVLIPPVAGGSDDTLLHIAIREEPIDDAVIT
                     QLRNLVPTAADGAVVVFVGQTRETPGTPAPGQGQAAAQHEGERVDRLEYEAFEEMALD
                     VLRAIGAEIEARFGVRRLAIVHRSGSVAVGEASVVIVAAAAHRAAVFDAARYAIEELK
                     ARAPIWKAEHFASGSVWLGAPAREGPSPE"
     gene            complement(3110..3937)
                     /locus_tag="VIF08_08520"
     CDS             complement(3110..3937)
                     /locus_tag="VIF08_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136067.1"
                     /translation="MRIVDSLPWVVSVLVFSLAFGSFAFVVVSRWRTDATRGYLGFTA
                     FCAGLLALFALATKLLGPPAATEEVVRIGPADLFMLRQVGLGVFAAASLLYPMFIRRA
                     RVTLVLELAGVAGGATALAAAAFGWAATAVDGVPLLVQLVVLSLATGGSMAAVILGHW
                     YLVTPKISEKPLVLQARLLLAVIGLQVVLFVVWTTLGGGPGQGAFDAFSGSNLLLVIL
                     RLVVTLMFPLALTWMAWRTALTRSMESATGLLYINLAAVMAGTIGAAALYISSGLLL"
     gene            complement(3937..4860)
                     /locus_tag="VIF08_08525"
     CDS             complement(3937..4860)
                     /locus_tag="VIF08_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY8136068.1"
                     /translation="MNSRPLKVGVQLPEVERLVGWPELRDMAHTAEDVGFDSLWVGDH
                     LLYRRPSGNVGPWEVWTTLAALAAATKRVEIGPLVAATSFHAPPMLAKMAATVDEISG
                     GRLILGLGAGWNEPDYTAFGFPYDQRVSRFEEAFTIIRTLLREGRIDFEGKFYTARDC
                     ELVPRGAPAQHPNGPPLMVGSTSRRMLAITAPHVDVWNEWHDKFANQPENLAPLMAQV
                     DEAARVAGRSPADIARTVTVLVHLTGGKGRSSSYAEPTPPHDGTKPAEMAATLRRYAD
                     AGISHVQLVLDPITVESIAECAPILEALDKG"
     gene            complement(4906..6567)
                     /locus_tag="VIF08_08530"
     CDS             complement(4906..6567)
                     /locus_tag="VIF08_08530"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY8136069.1"
                     /translation="MPLSPPPHRDDTPAPPSPLRLVRLRLRLAMLTMAVVPMAISMAL
                     INAVLTPMENGQAVYVLAVMVGLTLLLIALTVWMSRQVLRPAEELERSRTDLRKMYEV
                     ARADSLRDGLTGLGNHRAFQEELDRELEWYQRYKVPIALLLIDLDDLKLVNDSAGHAA
                     GDEMLREMGKLIAVSGRYADRAFRIGGDEFAILLPHTDVDGALQIARRLQERAMQPRS
                     GARPIPFSGGVSACPMLATTRAQLYAQADAALYWCKRHGRASIDVFHPVRDRAANQEA
                     SNELSAAIAKVVTDRLVRPVYQPIVDLTTGRVIGFEGLSRPTAESGFADPGSMFSAAE
                     AVGRTVELDLSCLHAVVAGARHMPVDQMLSINISPRTLEAPHFSADALMALLARYAIA
                     PDRVVVELTEREKVEDLNRLQANLGALQRHGLRIAADDVGAGNAGLRLLSQMRFDIVK
                     VDLSLVQEGTERDSSRAVLRSLRELAARWQAYVIAEGLETASQLRMVRELGITAGQGY
                     LLGRPMSEPNLLTVDLAALEAGGTVLSRPVALDERAMNAAAVGPN"
     gene            complement(6887..7240)
                     /locus_tag="VIF08_08535"
     CDS             complement(6887..7240)
                     /locus_tag="VIF08_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136070.1"
                     /translation="MGPLVGRPQRRVDRISRCLPDAHANTDQDPDYRSNSHTDPDTDT
                     HTDPDTDPHTHAHAHADAHAHADAHAHADSDSDADSDAYADSDAYADAYADSDAYADA
                     YTHATRVSNPDAHVA"
     gene            complement(7640..>9517)
                     /locus_tag="VIF08_08540"
     CDS             complement(7640..>9517)
                     /locus_tag="VIF08_08540"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HEY8136071.1"
                     /translation="GVTLGYDVTVSGILTLSSGNVTAAPYTLSIASTGSVSRTSGHIV
                     GNMRKHVATGAAVVTFEIGDATQYAPVTLTFASVDVAGDLIASTTAGDHTALASSAID
                     PAQSVNRFWSMSAIGLSFTTASADFTFGAGDLDAGADTTDFGVARYSSGAWDTLTTQT
                     QNPTGTSATGITAFGDFAVGELAPGALHHFTVTAPATAAVASAFDVTVTAVDASGNRV
                     GSYVGTVTFSSSDPHAVFSPASYTFLAADHGTRTFSAVATLYRAGTQTVSVSDGAITG
                     TSGAIAASAGAFVQLQILVPGEAADPGSVTGKTGSPTAQTAHTPFSVTVNAVDSYWNV
                     VIATDTVAITSSDANAVLPPNGALVDGSATFSVAAETGGATTVTATDMTDGTKTSSTS
                     PAIAVTNTAPVAAADSYEMMADNTLLVSAAGVLANDYDAESQPLTVGAPRPASGPAHG
                     SLTLNADGSFSYTPMAGYSGPDSFTYTAADVGMTSAPATVTITVRDHSLISTSGWGTS
                     FESSRFLDLTYPAYVAAGAIVDGATLNLSYRSLDGAGTLCYYIEVYNGSTLMATHGSA
                     GAPISCNSGPAYAADAIDLSEIVSADAANHVTIRIYMRDSAGARSQINGAMLAVSYSV
                     P"
BASE COUNT         1603 a         3132 c         3345 g         1437 t
ORIGIN      
        1 ggcgcagatc gatcgccatg gcgacctcaa ctcaacggtg atcgggccgt acgagacgcc
       61 caagacgcgg ctgcccggct ccgggggcgc gtgtgagatc gcccagaacg cgaagtcgat
      121 cttcgtgatc atgcgccagt cgccgcgctc gttcgttgag cggctcgact tccgcacctc
      181 ggtcgggacg cgcgtgcgcg tcgtggtgac cgacctcggc gtctaccacc cggtcgatgg
      241 cgagttgcag ctcgatgcgc tgcacgaggg cgtgacgctc gaccaggtca ggggcgcgat
      301 gggctggacg ccgcgcgtcg cctcgacgct cgcccagaca ccgccgccga gcgacgccga
      361 gctgcgcatg atccgcgagg agttggaccc ggcgggcgtc tacacgaagt agcattcgtc
      421 gccaaagcac gtcggcacct gaggagggac tcgtggccta ccgtcgcgca atcgcgctga
      481 tctgcctgct gatcacagcc tgcggctcgc ccactgcgtc acccacgtca caaccgacgg
      541 cagcgcccgg ctcaaccatg accggcgcgc ccacgatctc tcccgcaccg acagcgggat
      601 caacgcctgg cgcaaccgcg acccttgcgc ccactgacgt tcccaccagg gcaccaaccg
      661 acgcgccgcc gccattcgtg accccgacca cgtgggtcaa cgtcgcgtcg atgaaccacc
      721 cacggcgcgg caatgatgcc gtccggctgc tcgacggccg cgtcctcgcg gcgggtggcg
      781 aaacggagtc aacagacatc ctcgacagcg ccgaggtgta cgacccggct accgacacct
      841 ggacggatgt cgcgtccatg ggcgatcgcc gcgtctacgc gatcgttgtg ctgctcgctg
      901 atggccgcgt gctgcttgcc ggcggatacg gatctggcgg cgatgacctg ctgaccagcg
      961 cggagctgtt cgatccggcc acgaacacct tcagcccgac cggtccgatg ggtgtcgctc
     1021 gcgctcgctt ccagggagtg cgcctcgacg acggccgggt cctggtcatg ggcggcaaca
     1081 agcagggcgg tggcgcgctc gattcagccg aggtctacga cccgatagcc ggtacctgga
     1141 gccccaccgg atcaatgcac acgacacgcg aagccgggcg cgcagtcaag atggccgatg
     1201 gccgggttat cgtgacgggc ggctcggacg gcgttgggga cgggacaccg acgaactcga
     1261 ccgagatcta tgaccctcgg atcggcacgt tctcgcagat cggcccaatg acaatcgcgc
     1321 gcgaccgaca cgcaatcgtt ctcctgggtg acagccgcgt gatcctggcc ggcggacaga
     1381 gcaactccgg cgtcgagcgc gaaagcgaca tcttcgatcc gcgcagcggt gcgaccgttt
     1441 ggaccgcgac ggccccgttg ggcgatcgcc gcttcgcagc gtcctacgtc accatggcag
     1501 acggcaacgg actcgtgtgt ggcggcttta acggctcggc ctacctgcgc ggctgcgagg
     1561 catacgacag cgcgacagac agctggacca acacagagga cctgcacagc gcgacaagtg
     1621 gagcgttgat ggtgacgctc gcggacggcc gcgtgctcat ctgcggtggt ttgggcaagg
     1681 gcgacgtcac gctcaagacg tgcgagctct ttacggaggt cagcggctag ccgcggccgg
     1741 gcactcccgg acctcggcgc gccgacatca gcaagagcac tccgatgacg atgatcagca
     1801 cgggccacag cgcgcccagc tcagggatgc cggagattcg gcccgacgcc tggaccaacg
     1861 cgatcaggcc gaagatggcc agaccccaca gcggccaggt gctgcgcacg ccgcgcgaat
     1921 agtcgagcac ccagatcaac aggaaggcga cggcgagggc gcccgctgtc aggccggggc
     1981 cggagtagac gttcagctcg cgcgccaggc cggcgagggc gagggcgaga atgagcaatc
     2041 cgggtaccac cgcccagcgc gagcggccga acagccacgc cgcaagtaat gcgagcgcga
     2101 ttgccgtgag caccagggtc ccggagctga cgccgggcac gaaatactgg atcagcaggc
     2161 caacaccgac aagcacgagc aggacgccca gccaggggaa gctccagccg cgacggcccc
     2221 agccccacgc agcccattcg gggccctggg cacgctcgtt gtagtagttc cggtggaagt
     2281 cgtccggttg ggcgccgtcc ggtgtgacgt tgcggggctc agtcatagct caccatccac
     2341 aaagcataga gaaagggcgg gattgcctac tccggtgatg gaccctcgcg tgcgggcgcg
     2401 cccaaccaga ccgaaccgct cgcgaagtgc tcggccttcc agatgggtgc gcgggctttg
     2461 agctcctcga tggcatagcg cgcggcgtcg aagacagccg cgcggtgggc ggccgcggcc
     2521 acgatcacga cgctcgcctc gccgaccgcc acgctgccgc tgcggtggac gatcgccagg
     2581 cgccgcacac caaagcgtgc ctcgatctcc gcgccgatcg cccgcagcac gtcgagcgcc
     2641 atctcctcga atgcctcgta ctccagacga tcgacgcgtt cgccctcgtg ctgggccgcg
     2701 gcttgcccct ggcccggggc gggcgtccca ggcgtctccc gtgtctggcc cacgaagacg
     2761 acgaccgctc cgtcggccgc ggttggcacg agattgcgca gctgggttat cactgcgtcg
     2821 tcgatgggct cctcccggat cgcgatgtgc aacagggtgt cgtcgctgcc gccggccact
     2881 ggcgggataa ggacgagctc gtcaccgtcc tcgagccgcg tttccgccgc gctgtactcg
     2941 cggttgcgcg cgaagcgcac gacctcgcgt gagccggcga gcgcgggcgc gatcgcgacc
     3001 agcgcctgcc acgcgtcctc gatcgtcgcc ttctctggca gctcgatctg gcgctcgcgc
     3061 cagccggcct gctggcgcag gatcgcgaac aggcggacgg tgacgtgcat cagaggagca
     3121 gcccgctaga gatgtacaac gccgcggcac caatggtgcc cgccatgacc gccgccaggt
     3181 tgatgtacaa cagtccggtc gccgattcca tcgagcgcgt cagcgccgta cgccacgcca
     3241 tccaggtcag ggcgagcggg aacatcagcg tcaccaccag gcgcaggatc acaagcagca
     3301 ggttgctgcc gctgaaggcg tcgaacgcac cctggcccgg accgccgccg agcgtcgtcc
     3361 acacgacgaa caggacaacc tgaagcccga tcaccgcgag gagcagcctc gcctgcagca
     3421 ccagcggctt ctccgagatc ttgggcgtca cgagatacca gtgacccagg atcactgcgg
     3481 ccatcgaacc ccccgtcgcg agcgatagca cgacgagctg gacgagcagc ggtacaccgt
     3541 cgacggccgt agccgcccag ccgaacgcgg cagcagccaa ggcagtcgcc ccgcccgcga
     3601 cgccggcgag ctcgaggacc agcgttacgc gcgcgcgccg aataaacatc ggatagagca
     3661 acgatgccgc ggcaaagacc ccaagaccga cctgccgaag catgaacagg tcggccgggc
     3721 cgatgcgaac cacctcctcg gtcgcggccg gagggcccaa cagcttcgtc gccagcgcga
     3781 aaagcgcgag cagaccggcg cagaacgctg tgaaacccag gtagccgcgc gtcgcatcgg
     3841 ttcgccagcg gctgacgacc acgaaggcga acgagccgaa tgccagtgag aacacgagca
     3901 cagagacgac ccacggcagg ctgtcgacga ttcgcatcag cccttgtcga gcgcctcgag
     3961 gatgggcgcg cactcggcga tcgattcgac ggtgatggga tccagcacca actggacgtg
     4021 gctgatgccg gcgtccgcgt atcggcgcag ggtggcggcc atctctgcgg gcttcgtacc
     4081 gtcatggggt ggcgtgggct ccgcgtagga gctgctccgg cccttgccgc cagtcaggtg
     4141 aaccagcact gtgacggtgc gcgcgatgtc tgccggcgag cgaccggcga cgcgcgccgc
     4201 ctcatcgacc tgcgccatca gcggcgcgag gttctccggc tgattcgcga acttgtcgtg
     4261 ccactcgttc cacacgtcga cgtgcggtgc ggtgatggcg agcatgcgcc gtgacgtcga
     4321 gccaaccatc agcggcgggc cattgggatg ctgcgccgga gcgccgcgtg gcaccagctc
     4381 gcagtcgcgt gccgtgtaga acttgccctc gaagtcgatc cgcccttcgc gcagcaacgt
     4441 gcggatgatc gtgaaggcct cttcgaagcg gctgacccgc tggtcgtatg gaaagccgaa
     4501 ggcggtgtag tcgggctcgt tccagcccgc gcccaggccc aggatgagcc gcccgccgct
     4561 gatctcgtcg acggtcgccg ccatcttggc cagcatcggc ggggcatgga aggaggtcgc
     4621 ggcgacaagc ggtccgatct cgacgcgttt cgtcgccgcg gcgagcgccg cgagtgtcgt
     4681 ccagacctcc cacggaccga cattgccgct gggtcgccga tacagcaggt ggtcgcccac
     4741 ccacagcgaa tcgaagccga cgtcttccgc ggtgtgcgcc atgtcgcgca gctccggcca
     4801 gccaacgagg cgctcgacct ctggaagctg aactcctact ttgagcggcc ggctgttcac
     4861 gcggccgagt ctatcggctg ggcgcatggc cccaggccgg gacgcctagt tgggcccgac
     4921 cgcagccgcg ttcatcgcgc gctcgtccag tgccacaggc cgcgacagca ccgtgccgcc
     4981 ggcctcgagc gcggcgagat cgacagtgag caggttcggc tcggacatgg gccgcccaag
     5041 caggtatccc tgacccgcgg tgatgcccag ctcgcgcacc atgcgcagct gtgacgccgt
     5101 ctccagaccc tcggcgataa cgtatgcctg ccagcgtgcc gcgagctcgc gcagcgagcg
     5161 caggacggcc cgtgacgagt cgcgctcagt gccctcctgg accagcgaca ggtcgacctt
     5221 gacgatgtcg aaccgcatct ggctcagcag gcgcaggccg gcgttgccgg cgcccacgtc
     5281 atcggccgcg atgcgcaggc cgtggcgctg cagtgccccg aggttggcct ggaggcggtt
     5341 caggtcctcc accttttcac gttcggtcag ctccacgacg acgcggtccg gcgcgatcgc
     5401 ataccgggca agtagggcca tgagcgcgtc cgccgagaag tgcggcgcct ccagagtgcg
     5461 cggcgagata ttgatcgaca gcatctggtc gaccggcatg tggcgtgcgc cggcgactac
     5521 cgcatgcagg cacgaaaggt cgagctcgac cgtgcggcca accgcctcag ccgcgctgaa
     5581 catcgagccg ggatcggcaa agccggattc ggcggtcggg cgggacagcc cctcgaagcc
     5641 gatcacccgg cccgtggtca ggtcgacgat gggttgatac accggccgga ccagtcggtc
     5701 ggtgacaacc ttcgcgatcg cggcggacag ttcgttgctt gcttcctggt tggccgctcg
     5761 gtcccgaacc gggtggaaga cgtcaatcga tgctcggccg tgacgcttgc accaatagag
     5821 cgccgcatcc gcctgcgcgt agagctgggc gcgcgtcgtg gccagcatcg ggcacgctga
     5881 gacgccaccg gaaaagggga tgggccgggc gcccgagcga ggctgcattg cgcgctcctg
     5941 cagacggcgc gcgatttgca gggcgccgtc gacatcggtg tgcggcagca ggatcgcgaa
     6001 ctcgtcgccg ccgatgcgga aggcacgatc ggcgtagcgc cctgagacgg cgatgagctt
     6061 gcccatctcg cgcagcatct cgtcgccggc agcgtggccc gccgaatcgt tgaccagctt
     6121 gaggtcgtcc agatcgatca gaaggagagc gatcggcacc ttgtagcgct ggtaccactc
     6181 cagctcgcga tcgagctcct cctggaaggc gcggtggttg ccgagcccgg tcaggccatc
     6241 gcgcagcgag tctgcgcgcg caacctcgta catcttgcgc agatccgtgc gcgaacgttc
     6301 gagttcctcg gccggacgca ggacctggcg cgacatccag acggtcagag cgatcagcag
     6361 caacgtgagg ccgaccatca cggccagcac gtacacggcc tggccgtttt ccattggcgt
     6421 gagcacggcg ttgatcaggg ccatggagat ggccatcggc accaccgcca tcgtgagcat
     6481 ggcgaggcgc aaacgcaggc ggaccagccg caatgggctt ggcggggcgg gggtgtcgtc
     6541 gcgatgtggc ggcggcgaga ggggcacggg ttgcaggcac cagataaggg cgtgggaact
     6601 agttatccaa gaaccgttgc ccggcgcaca tagcccgaac gtcccccaag ggcgcggacc
     6661 ctcgggtggg gcgggccggg gcgcgattca gcctcggcgg acgggcctgt aggggctggt
     6721 cgaccacacg ccagccgcga ccggtctgcg cgcgacccag ccgcggcgcg tcctgcgggc
     6781 ggtcccgagc agggccacgg cggcggcgac cgccgcgatc aggagcacgg caatcaggga
     6841 ctgaccgttg ttgccgccac tccgacccga gctcgggctg gcggtgtcag gcaacgtggg
     6901 cgtcggggtt ggagacacgg gtggcgtggg tgtaggcgtc ggcgtaggcg tcggagtcgg
     6961 cgtaggcgtc ggcgtaggcg tcggagtcgg cgtaggcgtc ggagtcggcg tcggagtcgg
     7021 agtcggcgtg ggcgtgggcg tcggcgtggg cgtgggcgtc ggcgtgggcg tgggcgtggg
     7081 tgtgggggtc ggtgtcgggg tcggtgtggg tgtcggtgtc ggggtcggtg tgggagttgg
     7141 atctgtagtc ggggtcttgg tcggtgttgg cgtgggcgtc gggcaggcac cgtgatatcc
     7201 ggtcgacacg acgttgtggt cggccgacca gcggacccat acctggctga tcgacgacgc
     7261 ggtaaccacc gtctggcgca gcgttgtgct gtcaagcagg tacgtggtga agccggtgaa
     7321 cgatgagttc gtggcgaaat cgacgtggtg gtcggattcg gtgtggctgg cctggatggt
     7381 ccaggtgacg tgcgtcgcgt cagtgcacgt cccggaaact gccagcggcg accacgcgat
     7441 ggccggcccg gacagggcaa cccacgcgat gaaggtggcc gccagcaacc agccgagacg
     7501 ccttgggttc aaccttgacg gccgcacacg ggtgggcatt ccgtgggcca tgatagtggg
     7561 actttggtgt agtcgcgttg agtactttcg gcctagcgcc gttgccgccg cgcaacacgt
     7621 ggcgacgatg atctggtccc tacggcaccg agtagctgac cgccagcatc gcaccgttga
     7681 tctgcgaccg cgccccggct gaatcgcgca tgtagatccg gatggtcacg tggttggccg
     7741 catcggccga cacgatttcg gacaggtcga tcgcgtcggc cgcgtaggcc ggcccagagt
     7801 tgcacgagat gggcgcgccg gcgctgccat gcgtggccat gagcgtcgag ccgttgtaga
     7861 cctcgatgta gtagcacagc gtgcccgcgc catcgagtga tcggtaggac aggttcagcg
     7921 tggcgccatc gacgatcgcg cccgctgcca cgtacgcggg ataggtcaga tcgagaaagc
     7981 gcgagctctc gaaggacgtg ccccacccgg atgtcgagat cagggagtga tcacgcaccg
     8041 tgatggtgac cgtggccggc gccgaagtca tccccacgtc cgccgcggtg taggtgaacg
     8101 aatcggggcc cgagtagcca gccatgggcg tatagctgaa cgatccgtcg gcgttgagcg
     8161 tcaggctgcc gtgtgccggc ccactggcag gacgcggcgc accgacagtc aggggctgcg
     8221 actcggcgtc atagtcgttg gccaggacac cggccgcgct gaccagcagc gtgttgtccg
     8281 ccatcatctc gtagctgtcg gcggctgcga ccggggccgt attggttacg gcgatcgctg
     8341 ggctggtgga cgaggtcttg gtgccatcag tcatgtccgt ggcggtcact gtggtcgcgc
     8401 ccccggtctc cgcggcgacg ctgaaagtgg cgctgccgtc taccagggcg ccgttgggcg
     8461 gcaatacggc gttcgcgtcg gaagaggtga tcgcgacggt gtcggtcgcg atcacgacat
     8521 tccagtaact atcgaccgcg ttcacggtca cggagaacgg cgtgtgcgcc gtctgcgcgg
     8581 tcggcgagcc cgtcttgccg gtgaccgagc ccgggtcggc agcttcaccg ggcacgagga
     8641 tctgcagctg cacgaatgcc ccggcgctcg cggctatcgc gccactcgtc ccggtgatgg
     8701 cgccgtccga aaccgagacg gtctgggtgc ccgcgcggta tagcgtcgca acggcactga
     8761 acgtcctagt cccgtggtca gcggccagga acgtgtagct ggcgggcgag aagactgcat
     8821 gcggatcaga gctcgagaag gtgaccgttc cgacatagga gccgacgcgg ttgccggacg
     8881 catcgacggc tgtgactgtc acgtcgaagg cgctggcaac tgcagctgtg gccggcgcag
     8941 tgaccgtgaa gtggtgcaac gctccgggcg caagctcccc caccgcgaag tcgccgaagg
     9001 ctgtgatgcc ggtcgcgctg gtgccggtcg ggttctgggt ctgcgtcgtc agcgtgtccc
     9061 acgcgccgga tgagtagcgc gcgactccga agtccgtcgt atctgcgccg gcatcgagat
     9121 cacccgcacc gaatgtgaaa tctgcgctgg ccgtggtgaa cgacaagccg atcgcagaca
     9181 tgctccagaa gcggttgacg ctctgggcgg gatcgatggc cgatgacgca agggcagtgt
     9241 gatcgccagc ggttgtcgac gcgatcagat caccggcgac gtcaactgag gcgaacgtca
     9301 acgtgaccgg cgcgtattgc gtcgcgtcgc cgatctcgaa ggtcaccacc gcggccccgg
     9361 tcgcgacgtg tttgcgcatg ttgccgacga tgtggccgct ggtgcgtgat acggaaccgg
     9421 tcgacgcgat cgagagcgtg tagggagccg cagtgacgtt gcctgagctc aaggtgagga
     9481 tgccggacac ggtcacgtcg tagcccagcg tgacgcc
//
LOCUS       DATBRX010000223         3907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2885710, whole genome shotgun sequence.
ACCESSION   DATBRX010000223 DATBRX010000000
VERSION     DATBRX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2885710"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..208
                     /locus_tag="VIF08_08545"
     CDS             86..208
                     /locus_tag="VIF08_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136072.1"
                     /translation="MRGPAAAVERAKRMVDAAGATIVGGVDRLRRTVTWRREQD"
     gene            complement(205..1731)
                     /locus_tag="VIF08_08550"
     CDS             complement(205..1731)
                     /locus_tag="VIF08_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136073.1"
                     /translation="MTTDLALDTLEARGLIRLATAQPELEYLFRHALLQDTAYESLLK
                     QERKALHQVVGQALEQLYPDRVGEMAAVLARHFEQAGDTDKAVDYLSAAAKFAIDRNA
                     ITEAYELYGRASALLPPYDPADTASVRQRRIQVTLGRAQAGFTFLGDDEYLALLEPLV
                     EQSAALGDLRLEAEVHLAIALGRQMRGGRSLSDHALKRSLDRVDEIGRELNDPLIGAL
                     SSSIVGLYEIFSGRMRAGIARLEEVQPLLAQKRDFVGSSFALVALSFGYSRLGEWAKA
                     EEAVRFSRELAEQGDVVVRVDAMIGESTLHSIRGDLDKAVPLAMQCAQLAEEAGASAC
                     VVASNFVLGDAYMRQNQFGAAKIAFDRGDEIAQVTEQRIFRPSIAAYLRSLAASMGDY
                     RLGDKTFEQAIAEAREIGDIWGETTIVWKRAETELKRPAEQRNQAQMLADFETAYNSF
                     LEMAARPFWARVLREWGHALIATGAVAEGQTKLRDALAQLDELGIRSEADALRAELAA
                     "
     gene            complement(1728..3575)
                     /locus_tag="VIF08_08555"
     CDS             complement(1728..3575)
                     /locus_tag="VIF08_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8136074.1"
                     /translation="MSVDSVGLRCPRCDNPVSADARFCASCGQSLTPESDEAVAARQA
                     RLAAAAPAPLVEKMRAAKLTGERKVVTALFADVVGSTTLAESMDAEDWTQIINEAFDL
                     MSKAIFHYEGTIAQLQGDALLAFFGAPVAHEDDPERAVRAALDMIESINEYAAQLKRT
                     HDIDFRIRAGINTGPVIVGNVGSDLRYEYTALGDAVNVAARVQTAAQPGDVLISSMTQ
                     RYVEPVFEFEDLGDVQVKGKSEPVHLYRVTGLRAEAGPKRGLSGVGLSSPLVGRATEL
                     KTLTELFDVAKAGRGRLAVILGEPGLGKSRLLVELRNAVGADIADRGVPAAATWVEGR
                     CVSYGRALPYHLVFDLVWSLLGIPRGETGNVPRESIDARIDALLGADAADVKPYIAHL
                     LALPLNPAEADRLQLDPETLQGRYVSAIARIVRGLADKGPLVLVTEDIHWADPASVEI
                     LVQLVPLLTPLPVLGLFVSRVETDAPGWRLISQIKAGFGDAVTEIRLQPLDEQSSREL
                     VSNLLEIESLPEDVRQAILKRSEGNPFFVEEVIRMLIGRGAIVSAGDHWTATSDIGQV
                     EIPETLHGLLLARIDQLPDSAKRSLRVAAVIGRQFQVRVLERVLEESPA"
     gene            3685..>3907
                     /locus_tag="VIF08_08560"
     CDS             3685..>3907
                     /locus_tag="VIF08_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136075.1"
                     /translation="MATRETRRHKAHRRGHELLRGLITELRQERQTANVSQRAIATEL
                     GWQQAELNRLERFDFPNVPLVRLVEIAGVL"
BASE COUNT          625 a         1321 c         1338 g          623 t
ORIGIN      
        1 tacgcctcgc tgctgagctg ggtgggcatc ctgatcatcg tcgcgaccag cgtcctgtca
       61 tcgaccagga cgagccggtg acatcgtgcg cggcccggcc gcggccgtcg agcgagccaa
      121 gcgcatggtc gatgccgcgg gggcaacgat cgtgggcggt gtcgatcggc tacgccgcac
      181 cgtgacctgg cgcagggagc aggactaggc ggcgagctcc gcccgcagcg catcagcctc
      241 agagcggatg cccaactcat cgagctgcgc gagcgcgtca cgcagcttgg tctgaccttc
      301 cgcgacggcg ccggtcgcga tcagcgcgtg gccccactcg cgcagcactc gcgcccagaa
      361 cggtcgcgcg gccatttcca ggaacgagtt gtaggccgtt tcgaagtcgg ccagcatctg
      421 cgcctggttg cgctgctcgg caggtctctt gagctctgtt tccgcgcgct tccagacgat
      481 cgtcgtctcg ccccagatgt cgccgatctc gcgcgcctcg gcgatcgcct gctcgaacgt
      541 cttgtcgccc agccggtagt cgcccatgct ggccgcgagc gaccgcaggt acgccgcgat
      601 cgacgggcgg aagatgcgct gctcggtcac ctgggcaatc tcgtcgccgc ggtcgaatgc
      661 gatcttggct gcgccgaact ggttctggcg catgtacgcg tcgcccagga cgaagttgct
      721 tgccacgaca catgctgaag caccggcctc ctcggccagc tgggcgcact gcattgccag
      781 cgggaccgcc ttgtcgaggt cgccacgaat ggagtgcagc gttgactcgc cgatcatcgc
      841 gtcgacgcgg acgacgacgt cgccctgctc ggcgagctcg cgcgaaaagc gcaccgcctc
      901 ctcggccttg gcccactcgc ccaggcgcga gtagccgaac gacagggcca ccagcgcgaa
      961 cgacgagcca acgaagtcac gcttctgggc gagcagcggc tgaacctctt cgaggcgtgc
     1021 gatgcccgcc ctcattcggc cactgaagat ctcgtacaag cccactatgg agctggacag
     1081 cgcgccgatt aacggatcgt tcagctcgcg cccgatctcg tccacccggt cgagcgagcg
     1141 cttgagcgcg tgatcggaca acgaccgccc gccgcgcatc tgccgaccca gggctatcgc
     1201 gagatggact tccgcctcca ggcgcagatc gcccagggcc gcggattgct cgacgagcgg
     1261 ctcgagcagc gccaggtact cgtcgtcgcc aaggaaggtg aagccggcct gggcgcgacc
     1321 aagcgtgacc tggatgcggc gctggcggac cgacgccgtg tcggccgggt cgtacggcgg
     1381 aagcagcgcc gaggcgcggc cgtacagctc gtatgcctcc gtgatcgcgt tgcggtcgat
     1441 ggcgaacttg gcggctgctg acaggtaatc gaccgccttg tccgtatcgc ctgcctgctc
     1501 gaaatgtcgc gccagcacgg ccgccatctc gccgacacga tcgggataga gctgctcgag
     1561 cgcctggccg acgacttggt gaagggcctt ccgttcctgc ttgagcagcg actcgtatgc
     1621 cgtgtcctgc aacagggcgt ggcggaagag gtattcgagc tcgggctggg ccgtggcgag
     1681 gcggatgagt ccgcgggcct cgagcgtgtc gagcgcgagg tcggttgtca tgcgggtgac
     1741 tcctcgagca cgcgctccag gacgcgtact tggaattggc ggccgatcac ggcggcgacg
     1801 cgcagcgagc gcttggccga gtcgggcagt tggtcgatgc gcgcgagcag caggccatgc
     1861 agtgtctccg ggatctcgac ctggccgatg tccgaggtcg cggtccagtg gtcgccggcg
     1921 gagacgattg cgccgcggcc gatgagcatc cggataactt cctcgacgaa gaacgggttg
     1981 ccctcggagc gcttcaagat ggcctggcgc acgtcctcgg ggagcgactc gatctcgagc
     2041 aggttcgaca ccagctcgcg cgatgactgt tcatcgagcg gctgcaaccg gatctcggtg
     2101 accgcatcgc cgaagccggc cttgatctgc gagatgagcc gccagccggg cgcgtcggtc
     2161 tcgacgcggc ttacgaagag gcccagcacc ggtagcggcg tgagcagcgg caccagctgg
     2221 acgaggatct cgaccgacgc tggatcggcc cagtggatgt cctccgttac cagcacgagc
     2281 gggcctttgt ccgcaaggcc gcgcacgata cgtgcgatgg cgctgacgta gcgcccctgc
     2341 agggtttccg ggtccagctg caggcggtcc gcctcggccg ggttgagcgg cagggcgagc
     2401 aggtgggcga tgtacggctt cacgtcggcc gcgtccgcac ccagcagcgc gtcgatgcgc
     2461 gcgtcgatcg actcgcgcgg cacgttgccg gtctcgccgc gcgggatgcc gagcagcgac
     2521 cagaccaggt cgaacacgag gtggtagggc agggcgcgcc cgtagctgac gcagcggccc
     2581 tcgacccagg tcgcggcggc cggcacgccg cggtccgcaa tatccgcgcc gacggcgttg
     2641 cggagctcga cgagcagtcg cgacttgccc aggcccggct cgcccaggat caccgccagc
     2701 cgaccgcggc ccgccttggc cacgtcgaac agctcggtca gcgtcttcaa ttcagttgcg
     2761 cgccccacca gggggctcga aagaccaacc cccgacaaac cgcgcttagg cccggcctcg
     2821 gcacgcagtc ccgtcacccg gtagaggtgc accggctcgg atttgccctt gacctggacg
     2881 tcgcccaggt cctcgaactc gaagacgggt tcgacgtagc gttgggtcat ggagctgatc
     2941 agcacatcgc ccggctgggc cgcggtctgg acgcgggcgg cgacgttcac ggcgtcaccg
     3001 agtgccgtgt actcgtagcg caggtcggag ccgacgttgc cgacgatgac cggcccagtg
     3061 ttgatgccgg cgcggatgcg gaagtcgatg tcgtgcgtgc gcttgagctg cgcggcgtac
     3121 tcgttgatcg actcgatcat gtccagcgcc gcgcgaacgg cgcgctccgg atcatcctcg
     3181 tgagccaccg gcgcgccgaa gaatgccagc agggcgtcac cctggagctg ggcgatcgtg
     3241 ccctcgtagt ggaagatcgc cttggacatg aggtcgaacg cctcgttgat gatctgtgtc
     3301 caatcctcgg cgtccattga ttcggcgagc gtcgtggagc cgacgacatc cgcgaacagc
     3361 gcggtcacga ccttgcgctc gccggtcagc ttggccgcac gcatcttctc cacgagcggc
     3421 gccggggcgg cggccgcgag ccgcgcctgg cgcgcggcga cagcctcgtc gctctcaggc
     3481 gtgagcgact ggccgcagct cgcgcagaag cgcgcgtcgg ccgaaacggg gttatcgcag
     3541 cgtgggcatc tcagcccgac cgagtccacg gacatccagc cgtgatctta cgccgcagcg
     3601 actcaggtac atctcggctg atcagtcaca cacgtcaatc gtccgataag tgggcgtgaa
     3661 gccgagttga tcacgcttgt tcccgtggca acccgggaga cacgcaggca caaggcgcat
     3721 cggcgcggcc acgagctgct gcgcggcttg atcaccgagc ttcgccagga gcgccaaacg
     3781 gccaacgtga gccaacgtgc gatcgccacg gaacttggct ggcaacaggc cgagctcaac
     3841 cgcctggagc gcttcgactt tccgaacgtc cctctcgtcc ggctcgtgga gatcgccggc
     3901 gttctgg
//
LOCUS       DATBRX010000224         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_877776, whole genome shotgun sequence.
ACCESSION   DATBRX010000224 DATBRX010000000
VERSION     DATBRX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877776"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VIF08_08565"
     CDS             <1..619
                     /locus_tag="VIF08_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY8136076.1"
                     /translation="AGALAGYAIAIPVGAIAVLIIHIGLTQGLRQGLAAGAGAATADL
                     IYATIAALAGAGVAALVGPLEGPLRVVGGIVLVLIGVYGLMSAWQSRDRDTGAHLPAH
                     RPHGRTYLAVLGLTLLNPATVIYFAALTVGLPFLGDISERLVFAGAAFLASLSWQSLL
                     AVFGALLGRGAGHRLRLPTVLLGNFIVIALGLLVLAGTLAPTATS"
     gene            626..1120
                     /locus_tag="VIF08_08570"
     CDS             626..1120
                     /locus_tag="VIF08_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136077.1"
                     /translation="MRVGVLMAVLLLAACAGASPSSAPGAVRILASDLLLIEGPFTVA
                     VIDNATDFEAGWARSSASGPPPVVSFATEVVVYLGMAGSSSCPETFQHLVVDAEAAHV
                     YGEWDSSQFVGKPCSDDLRSQGVVLAVRRTVLPPTEFTLTLRAELICPDCPDHPDQVL
                     VDLP"
     gene            complement(1117..2157)
                     /locus_tag="VIF08_08575"
     CDS             complement(1117..2157)
                     /locus_tag="VIF08_08575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8136078.1"
                     /translation="MSDWPTAAALADELGVGILLIGNNDRVAAANATAQRLLGEGGLV
                     GKSLIEAFVDHHLEEAIRNARNQATRMDYTPPTDRQVTLEVRAGRAANDEHVTVTLTD
                     VSELRRLRRIRSEFIDNLSHELRTPLTTMRLLAESLSMETKRTSVAPRVTDMINRVDL
                     ETARLVQMVDELLDLATIEQGEAPLEMAPVDLGAVVDETLERLRGYAEHHSVSLQADV
                     SPETGTRTVSGDADRLGQILINVVDNAIRYSPVGGEVVVHVRPSESEVVVEVTDQGAG
                     IAQADLERIFERFYKGDRARSRGRGGTGLGLAIARHIAERHGGRMWARSEPGKGAQLF
                     LALPRAEPARAG"
     gene            complement(2154..2792)
                     /locus_tag="VIF08_08580"
     CDS             complement(2154..2792)
                     /locus_tag="VIF08_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002068740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8136079.1"
                     /translation="MAYQLEQEGYRVVQASDGAQALERFRADKPDLVLLDLMLPRLSG
                     IEFTRIVRRESDAPILMLTAKAGEVDKVVGLELGADDYVTKPFSLRELLARVNGLLRR
                     HEKAASTSEPTEPVSLGKVTVDLAGHRLMRGTEIRPLKPKAFELLAFLMRNPGQVFSR
                     EQLLSEVWGYDFAGETRTVDVHVHWLRREIETDPANPALLQTVRGVGYVLRK"
BASE COUNT          383 a         1004 c          923 g          491 t
ORIGIN      
        1 cgccggtgcc ctagccggct acgccatcgc catcccggtc ggcgcgatcg ccgtgctgat
       61 catccatatc ggcctgaccc aaggtctgcg ccagggactc gcggcgggag caggggcagc
      121 tacagcggac ctcatctacg ccacgatcgc cgccctggcg ggagcgggtg tggccgcgct
      181 ggtcggtccg ctcgagggtc cattgcgggt ggtcgggggc atcgtcctcg tgctcatcgg
      241 tgtgtacgga ttgatgtctg cctggcagtc gcgcgaccgc gacaccggcg cgcacctgcc
      301 agcccatcga ccccatggcc gcacctactt ggcggtgctg ggattgacgc tcctcaatcc
      361 cgcgaccgtc atctacttcg ccgcgctcac cgtgggcctg cccttcctgg gtgacatcag
      421 tgagcggctg gtgttcgccg gcgcggcctt cctcgcctcg ctctcctggc agtcgctgct
      481 cgccgtcttt ggcgcccttc ttggtcgcgg cgctggccac cggctgcgcc tcccgaccgt
      541 gctgctcggc aacttcatcg tgatcgcgct gggcctgctg gtcctcgccg gaacactcgc
      601 gcccacggcc acgtcatagg ccgagatgcg cgtcggcgtt ctgatggcgg tcctcctcct
      661 cgccgcctgc gccggcgcgt ctccttcctc cgcgcccggc gccgtgcgga tcctcgccag
      721 cgacctgctg ctgatcgagg gcccgttcac ggtcgccgtg atcgacaacg cgaccgactt
      781 cgaagccggc tgggcgcgct cgagcgcctc cggaccgcca cctgttgtca gcttcgccac
      841 cgaggtggtc gtatatctgg gcatggccgg ctcctcgagc tgtccggaga ctttccagca
      901 cctcgtggtc gatgctgagg ccgctcacgt ctacggcgag tgggacagca gccaattcgt
      961 cggcaaaccg tgctcggacg acttgcgcag ccagggcgtc gtgctcgcgg tcagacgcac
     1021 ggttctgccg cccacggagt tcacgctcac gctgcgcgca gagctgatct gccccgactg
     1081 tcccgaccac cccgaccagg tgttggtcga cctgccctag ccagctctgg ctggttccgc
     1141 gcgcggcagc gcgagaaaca gctgcgcgcc cttgccgggc tcgcttcggg cccacatccg
     1201 cccgccatga cgctcggcga tgtggcgcgc gatcgccagc ccgagacccg tgccgccgcg
     1261 gccccgtgag cgggcgcgat cacccttgta gaagcgttcg aagatccgct cgagatcggc
     1321 ctgagcgatg cccgctccct ggtcggtgac ttcgaccacc acctccgact cggacggacg
     1381 gacatggacg acgacctcgc cgccgacagg cgaatagcgg atcgcgttgt cgaccacgtt
     1441 gatcaggatc tgacccaggc gatctgcgtc gcccgacacc gtgcgcgtgc ccgtctccgg
     1501 cgagacatcg gcctgtagcg agaccgagtg gtgctcggcg tagccgcgca gccgctcaag
     1561 cgtctcgtca acgaccgcgc ccaggtccac cggtgccatc tcgaggggcg cctcaccctg
     1621 ctcgatcgtg gccaggtcga gcagctcgtc gaccatctgc acaaggcgcg cggtctcgag
     1681 atcgacgcga ttgatcatgt cggtcacgcg cggcgcgacg ctggtgcgct tggtttccat
     1741 ggatagcgac tcggcgagca ggcgcatcgt cgtcaacggc gtgcgcagct cgtgggagag
     1801 gttgtcgatg aattcgctcc ggatgcggcg caggcggcgc agctccgaca cgtcagtcag
     1861 cgtgaccgtg acatgctcgt cgttcgccgc acgcccggcg cggacctcga gcgtcacctg
     1921 gcgatcggtc ggcggcgtgt agtccatgcg cgttgcctgg ttgcgcgcgt tccggatcgc
     1981 ctcctcgagg tgatgatcga cgaacgcttc gatgaggctc ttgccgacga ggccgccctc
     2041 accgagcaac cgctgcgccg tggcgttggc cgcagccacg cgatcgttgt tgccgatcaa
     2101 caggatgccc acgcccagct cgtcggccag cgctgcagcg gtgggccagt cgctcatttg
     2161 cgcaggacgt agcccacgcc gcgcacggtc tggagcagcg ccgggttggc ggggtcggtc
     2221 tcgatctcgc gccgcagcca gtgcacgtgc acgtccacgg tgcgcgtctc gccggcaaag
     2281 tcgtagcccc acacctcgct cagcaattgc tcgcggctga acacctggcc cgggttgcgc
     2341 atcaggaacg ccagcagctc gaaggccttg ggcttgagcg gccggatctc agtcccgcgc
     2401 atgagtcggt gcccggccag atcgacagtg accttgccca ggctgaccgg ctccgtgggc
     2461 tcgctcgtgg aagcggcctt ttcgtgacgg cgtagcagcc cgttcacgcg ggcgagcagc
     2521 tcgcgcaggc tgaacggctt cgtgacgtag tcgtcggcgc ccagctccag gccgaccacc
     2581 ttgtcgacct cgcccgcctt cgcggtcagc atcaggattg gcgcgtccga ttcgcgtcgg
     2641 acaatgcgcg tgaactcgat gcccgacagc cggggcagca tcaggtcgag cagcaccagg
     2701 tcgggtttgt cggcccggaa gcgctccagc gcctgggcgc cgtcgctcgc ttggacgacg
     2761 cgatagccct cttgctcgag ctggtacgcc aattcagtgc g
//
LOCUS       DATBRX010000225        12024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2441788, whole genome shotgun sequence.
ACCESSION   DATBRX010000225 DATBRX010000000
VERSION     DATBRX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441788"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VIF08_08585"
     CDS             <1..256
                     /locus_tag="VIF08_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136080.1"
                     /translation="GRLDKSVYEYSRPCYPEVIYIQGALYLERYRAAVGDEPFWAGMR
                     QFYDMYRFGIGGTRRFLDTLDAASGYDSELHARRFPSLYR"
     gene            complement(258..683)
                     /locus_tag="VIF08_08590"
     CDS             complement(258..683)
                     /locus_tag="VIF08_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136081.1"
                     /translation="MTDTDDLARTLFRLETSPLTTAAVTARITRAVIEWAAGRGWAAR
                     TEARVGVGEATRLGFVDVIVKRGENQPDLAIEIDSTHKPWSVDKLRHAVAAGMEGIWI
                     RWGDDEWAGAFDDVDVIQLPVTRRTRSRARPRTQLGLWQ"
     gene            complement(719..804)
                     /locus_tag="VIF08_08595"
     tRNA            complement(719..804)
                     /locus_tag="VIF08_08595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(768..770),aa:Ser,seq:tga)
     gene            complement(841..2001)
                     /locus_tag="VIF08_08600"
     CDS             complement(841..2001)
                     /locus_tag="VIF08_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8136082.1"
                     /translation="MTAPLILDIGLVLLAAAAGGWLARRIGLPAVVGYLAVGLLISPF
                     TPGYVADRDQIHLLADVGVALLLFEVGIEINIDRLRKEHGALIAVPPLQVIVTLAISA
                     AALMMAGLTPAAAALVGLGIASSSSVVVVNITRSRRRTTDPPTERAMLGLSVVQDIAV
                     VVASVVLFGLIGINNREPLVALLQFGAFVLVAVIVAWILPRVLRELRGESDHFLIVSV
                     AVALAVAALGDIVFGVPLALAAFVAGLAISEGPTTSEARRRLLPFRDLFAVLFFVAVG
                     SLIDPAALMTALPALGLVIGLVIVAKVGVNYLLARAGGLPRPLQLAVGLGQVGEFSFV
                     IVSVGVAAGVVPAAWFAATLGAVVITIAVSAVVARMVGPQKTPGPTATAAGL"
     gene            2112..2285
                     /locus_tag="VIF08_08605"
     CDS             2112..2285
                     /locus_tag="VIF08_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136083.1"
                     /translation="MTRHPIEYRCDLCRRHVEDDLRSRARRGWESLIICDSCLRQAGS
                     IDDRRATPEAVAA"
     gene            complement(2310..3584)
                     /gene="serS"
                     /locus_tag="VIF08_08610"
     CDS             complement(2310..3584)
                     /gene="serS"
                     /locus_tag="VIF08_08610"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY8136084.1"
                     /translation="MSVGMQRLRDDAETIRRGATAKGYDAATVDQALAADEERRKLLS
                     EIDAWRSERKKVSEQVGTAMKSGATNAEELRAKSVELGSSIDEGEERLRSVEATLEEL
                     LLRIPNPADADAPVGSPDATTTVRSWGEPLAHDAGGWERRPHWEIGERLGMLDLAAGA
                     KVAGSGFPIYRGAGARLERALINLMLELQTTQGGMTEVWPPAVVNQDSARGTGQIPDK
                     EEQMYVVTRDDLYLVPTAEVPVTNIHRDEIIDAARLPIRYVAYSPSFRREAGAAGKAT
                     RGILRVHQFDKVEMVSFVRPDESAAELERMTECAEAALQLLGLPYRVRLLSTGDMGFT
                     QAKTYDIEVWAPGVQEWLEVSSVSNFRDFQARRMNIRYRPEAGAKPEILHTLNGSSLG
                     FPRTFAALLENYQLQDGGLALPEPLASRVGNI"
     gene            complement(3581..5194)
                     /gene="lysS"
                     /locus_tag="VIF08_08615"
     CDS             complement(3581..5194)
                     /gene="lysS"
                     /locus_tag="VIF08_08615"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8136085.1"
                     /translation="MTSPPANRAGQAYWADEIAGQAVGPQVINDSKTPSGTVHVGSLR
                     GVVLHDALRRAIAERGTEVEFRYGVEDLDPMDAQAMLSGDAIDRYMGVPLINVPAPEG
                     SAQPNYARHFVTLFLETFTDLGIKPTLYWMSENYRSGAMDPYIHKALDHADVILDIYR
                     TVSTVNHPNGWLPISVICENCGKIGTTAPRDWDGSTVAYECRPDLVTWARGCGFSGRV
                     TPFGGHAKLVWNVDWAARWGLVGATIEGCGKDLATAGGSRDRADAISRRVFEREPPLN
                     IPYEFLNIGGKKMSTSKGTGAAAHEIAALLPPTQLRFLFLRHRPNRALDFDPAGDTLP
                     RLFDDFDRIADSVAGKPTRGELPPDPERIFALSLADPAADPAAVAAQYRPPFGHLSLL
                     LQVPGTDIRARLDTEKGTPLDAAEQAVASERIAAAQIWLEDFAPDSARFAIDMEKVPD
                     AAADLTADQRRYLGLLAAAARAQTPSNGETWQALIFETAKGNGFAPANAFAALYLAFM
                     GRSNGPRAGWLLAALDAEFVIGRLQEAAR"
     gene            complement(5196..5660)
                     /gene="greA"
                     /locus_tag="VIF08_08620"
     CDS             complement(5196..5660)
                     /gene="greA"
                     /locus_tag="VIF08_08620"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8136086.1"
                     /translation="MNNKPAYLSKEGLEQIRKELEELVNVRRAEIAARIHEAKEHGDI
                     TENAEYEDAKNEQAFVEGRIQALSALVKNAVVIEENRSSTHVQIGSTVTIQSKDGKES
                     FMIVGSAEASPAEGRISNESPVGRALLGRKKGEEITVSVPAGDSKYKILSIH"
     gene            complement(5799..6266)
                     /locus_tag="VIF08_08625"
     CDS             complement(5799..6266)
                     /locus_tag="VIF08_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136087.1"
                     /translation="MKRLRQQQIRDLLGTREIATQHELAAALRERGFRATQATVSRDV
                     AEMGLVKVSRNGTRHYSLPPRSGPPETSGDERLALILRDLPIEVRPSGLMLIIRAVPG
                     SAHAIAAALDRTGWPEVAGSIAGDDTVFIACSDRKALDRVRARLVQLASLGVG"
     gene            complement(6344..7165)
                     /gene="uppP"
                     /locus_tag="VIF08_08630"
     CDS             complement(6344..7165)
                     /gene="uppP"
                     /locus_tag="VIF08_08630"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEY8136088.1"
                     /translation="MIFQAIVVGIVQGLTEFLPVSSSAHLILVPRVLGWDNAFILSPA
                     FDATLHLGTVVALIAYFWRDLWRYFVAFLRMLRDRSIAGDADRKVALLLAISIIPGAL
                     LGVGLEGFVDTFFREQLLVVCALLVVGAVVLFVAERIARHTREMDELRPVEALGIGLA
                     QAFALFPGISRSGITISAGLILGLKRDAAARFSFLMATPIIAGAGLWKLRELVGGSTV
                     EFNGAALAAGLIAAAISGLIAIWFLLAYLRRASTDLFGIYRVGAAIVFAVLLLAR"
     gene            complement(7203..9422)
                     /locus_tag="VIF08_08635"
     CDS             complement(7203..9422)
                     /locus_tag="VIF08_08635"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY8136089.1"
                     /translation="MVLASALQWIIPIAGIAAVLFAGYLARDVLRRDTGTDAMRDVAS
                     TIYEGAVAFIRRQYTTIGLLALGGAVIIGIVIYLVETPDVADVPSLAGAPIAIMTAIA
                     FLVGAACSMASGIIGMFISVKSNVRTAAAARRSLVEAVQVAMRGGAVSGFLVVALSLL
                     GVWGIFTAYESLVGEVSVAEAPFLIVGFGFGASFVALFAQLGGGIYTKAADVGSDLVG
                     KVEAGIPEDDPRNAGVVADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGVFAIAS
                     IANWPNPEAWIFFPLVVRAFGLLATIVAIFFVRGREDENPMNMLNRGYWVTTILSVAA
                     LAFVTYVMMSTGPVNAGAMGIPTWVWFFGCGVVGLATSVAFVYITQYYTAGSFRPVRE
                     IAEASKTGPATNIISGTAVGYETTAVTAISIGIALLASHWLGSQAFANDPQSAAIGGI
                     FGTAVATMGMLMTTAYILAMDTFGPITDNAGGIAEFSRSEASAREITDRLDAVGNTTK
                     ALTKGYAIASASLAAFLLFSAYISKVNLIQTNRGGELITSVNLADVSVFIAALIGAML
                     VYFFSSLAIRAVGVTAQSIIVEVRRQFREMPGIMDYTQRPDYARVVDITTRAALRQMV
                     LPGVVAVATPILVGVLLGAEAVAGMLMVGTIAGVLLATVLNNGGGAWDNAKKYIESGH
                     LLDADGKVLGKGTPAHAAAVVGDTVGDPFKDTAGPSLHVLVKLLATITLVLAPLFIR"
     gene            9611..10240
                     /gene="lepB"
                     /locus_tag="VIF08_08640"
     CDS             9611..10240
                     /gene="lepB"
                     /locus_tag="VIF08_08640"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8136090.1"
                     /translation="MSRRPLGCLIEIAETLVLTIIIFWLIQTFVAQPYRVEQESMRST
                     LEPNQYVLVDKLSPHFDSYSRGDIVVFNPVLRDECSADVVGGLTDVTPYIKRVIGEPG
                     DRVELLDGGVTVNGEPLDEPYVHGAHTTPLSGETAWNVPADRLFVMGDNRQNSTDSRS
                     DQIGMICTRDVIGRAWLRYWPIDTLGIMQTPTYPNVPAASPLPTASPAP"
     gene            complement(10266..10718)
                     /gene="ndk"
                     /locus_tag="VIF08_08645"
     CDS             complement(10266..10718)
                     /gene="ndk"
                     /locus_tag="VIF08_08645"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY8136091.1"
                     /translation="MAERTLILIKPDAVQRQLIGKIVDRYEARGLRICGMKMVHADRR
                     LAERHYAVHREKPFYAGLVEFITSAPLVAMAVEGPNAIAVCREINGKTRPHEAAPGSI
                     RGDWAVDTGHNLVHASDSPENAAAELELWFEADELIDYPRDIDRWVLD"
     gene            complement(10730..12007)
                     /locus_tag="VIF08_08650"
     CDS             complement(10730..12007)
                     /locus_tag="VIF08_08650"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8136092.1"
                     /translation="MTDEAPFERTALPNGPRVISAQLPGSRSLSVAIYVLAGSRLETR
                     EHAGVAHFMEHVTFKGTTAYPTTRAVSEAIEGCGGSSNAATDRESTIYWARLPVREAE
                     RAFSVLSELVFRPLLREADIARERDIIVEEIRSYQDDPGQHVFNQFDRAFFGDTPLGW
                     EIAGDEASVRGLTVDQIKGFWSETYRPANTVVAVAGDLDHATIVDLVKRNVGTGNGVV
                     RGFSGVPALPVERLSVEQRDLAQAHLVLGVPALRRDDPEQWTLEMLNTILGEGSSSRL
                     FQELREEAGLAYDVHSFQTDFADCGTLQIYAGVDPADLESATEAILAELRRMVDEPVA
                     MDELDRARAYASGRLELRLEESRHLSAWLGIQEALHERVLTLDQALAELDKVTPEGIQ
                     SLAQRLFTDDRLCMAVISPRGTAKAVERVLHLN"
BASE COUNT         2058 a         4007 c         4077 g         1882 t
ORIGIN      
        1 cggacggctg gacaagtcgg tctatgagta cagccggccc tgctacccgg aagtcatata
       61 catccagggc gcgctttatc tggagcgcta ccgcgcagcg gtaggcgacg agccattctg
      121 ggcaggcatg aggcagttct acgatatgta ccggtttggc attggcggca cgcgccggtt
      181 cctggacacg ctcgatgccg cctcgggcta cgacagcgag ttgcatgcca gaaggttccc
      241 aagcctctat cgctgactca ctgccacagg cccagctggg tacgtggccg cgcacgagat
      301 ctcgtccggc gcgtcaccgg cagctggatg acgtcgacat cgtcgaaggc gcccgcccat
      361 tcgtcgtcgc cccagcggat ccatatgccc tccatgccgg cggccaccgc atggcgcaac
      421 ttgtcgaccg accatggctt gtgggtactg tcgatctcga tcgccaggtc gggctggttc
      481 tcgccacgct tgacgatgac gtccacgaag ccgaggcgag tggcctcccc cacgccaacg
      541 cgtgcctccg tccgcgcggc ccagccgcgg ccagcggccc attcgatgac cgcgcgcgtg
      601 atgcgtgccg tcacggccgc cgtcgtgagc gggctggtct cgagtcggaa gagtgtgcgg
      661 gccagatcgt cagtgtcagt cacgagggca tgctcgtaat cggtcgtgac agcttgtgtg
      721 gcggagaggg agggattcga acccccggag ctttcgctca acggttttca agaccgccgc
      781 cataaaccgc tcggccacct ctcctgtgag atgaggatac cgctggcaag cggcggccgg
      841 tcagaggccc gctgcggttg cggtcggtcc cggtgtcttc tgcggcccga ccatccgcgc
      901 cacgacggcc gacaccgcga tggtgatcac gaccgcgcct agcgtggccg cgaaccaggc
      961 agccggcaca accccggctg cgacgccgac tgagacgatc acgaacgaga actccccgac
     1021 ttggcccagg cctacggcca gctggagcgg ccggggcagg cctccggcgc gcgctaggag
     1081 gtagttcacg ccgaccttgg cgacgatcac gaggccgatg accaggccga gcgccggcag
     1141 ggccgtcatg agcgcggccg gatcgatcag cgagcccacg gcgacgaaga acagcacagc
     1201 gaagaggtcg cggaacggca ggagacgacg gcgcgcctcg ctcgtcgttg gcccctcact
     1261 gatcgccaac ccggcgacga acgcggccag ggcaagcggc acgccgaaaa cgatgtcgcc
     1321 cagcgccgca acggcaagcg caaccgcgac ggacacgatc aggaagtggt ccgactcacc
     1381 gcgcagctcc ctcaagacgc gcggcaggat ccaggccacg atcaccgcta ccaaaacgaa
     1441 tgcgccgaac tggaggagtg cgactagagg ctcccgattg ttgatgccga tcagcccgaa
     1501 caggacgacc gaggccacca cgaccgcgat gtcctggacc acgctcaggc cgagcattgc
     1561 ccgctcggtc gggggatctg tcgtgcgccg tctgctccgg gtgatgttga ccacgacgac
     1621 gctcgacgaa gaggcaatgc ccaggcccac tagtgccgcc gcagccggcg tcaggccggc
     1681 catcatcagc gcagcggcgg agatggcgag ggtgacgatg acctggagcg gaggcaccgc
     1741 gatgagcgcg ccgtgctcct tgcgtagccg atctatgttg atctcgatgc ccacctcgaa
     1801 cagcagcagg gcaacgccca cgtccgccag caggtggatc tggtcacggt ccgcgacgta
     1861 acccggcgtg aagggcgaga tgagtagacc gacagccagg tagccgacca ctgcgggcag
     1921 gccgatccgg cgcgccagcc agccacccgc cgcggccgca agcagcacca ggccgatgtc
     1981 gaggatcagc ggggcagtca ccggcggagg ttacgggcga cgatgggcag tcggcaaccc
     2041 gtgccggttg gcccccacag cgcgaccaac ggcccatcct cgtggctcgc gccagacgcg
     2101 atccttcgtc catgacacga cacccaatcg agtaccgctg cgatctgtgc cgacggcacg
     2161 tggaggacga tctccgctcc cgcgcccggc gcggttggga atcactgatc atctgcgact
     2221 cctgcctgcg ccaggccgga tcgatcgatg atcggcgcgc gacgccggag gcagtcgccg
     2281 cctgaggcgc ggctgcggga acgcggcctc tagatattgc cgacgcgcga ggcgagcggc
     2341 tccggtagcg ccagcccacc atcttgtagc tggtagttct ccagcagcgc cgcgaacgtg
     2401 cgcgggaaac ccagcgatga gccgttgagg gtgtgcagga tctccggctt ggcgccggct
     2461 tcgggtcggt agcggatgtt catccgccgc gcctggaagt cgcggaagtt ggacacgctc
     2521 gaaacttcca gccactcctg cacgccgggc gcccagacct cgatgtcata cgtctttgcc
     2581 tgggtgaagc ccatgtcccc ggtcgacagc agccgaacgc ggtagggcag ccccagcagc
     2641 tgcagcgcgg cctcggcgca ctcggtcatg cgctccagct ccgcggctga ctcgtccggc
     2701 ctgacgaacg acaccatctc gaccttgtcg aactggtgca cgcgcaggat gccgcgcgtc
     2761 gctttgccgg ccgcgcccgc ctcgcgccgg aacgacggcg agtaagccac gtagcggatg
     2821 ggcaggcgtg cggcgtcgat gatctcgtca cggtggatgt tggtgacggg cacctcggcc
     2881 gtgggcacca ggtagaggtc gtcgcgagtc acgacgtaca tctgctcttc cttgtcgggg
     2941 atttggcccg tgccgcgcgc cgaatcctgg ttgacaaccg cgggcggcca gacctcggtc
     3001 atgccgcctt gagtcgtctg cagctcgagc atcaggttga tcagcgcgcg ctcgaggcgg
     3061 gcgccggcgc cgcgatagat ggggaacccg ctgccggcca ccttggcgcc ggcggcgaga
     3121 tccagcatgc ccaggcgctc cccgatctcc cagtgcggcc tgcgctccca accaccggcg
     3181 tcgtgcgcca gcggctcacc ccagctgcgc accgtcgtcg tggcgtccgg cgagccaact
     3241 ggagcatccg cgtcggccgg gttggggatg cgcagcagca gctcctcgag ggttgcctcg
     3301 accgaccgaa gacgctcctc cccttcgtcg atgctggagc ccagctcgac cgacttggcg
     3361 cgcagctctt cggcgttcgt ggccccggat ttcatggccg tgcccacctg ctcgctgacc
     3421 ttcttgcgct cggagcgcca ggcgtcgatc tcgctgagca acttgcgccg ctcctcgtcg
     3481 gcggcgagtg cctgatcgac ggttgccgcg tcgtagccct tggcggttgc accacgacga
     3541 atcgtttctg catcgtcgcg taggcgctgc atgccgacgc tcatcgcgcg gcctcctgga
     3601 gccggccaat cacgaactcg gcatcgagcg ccgcgagcag ccagcccgcg cgcggcccat
     3661 tggagcgacc cataaacgca agatagagcg ccgcgaaggc attcgcgggc gcaaatccgt
     3721 tgcccttggc ggtctcgaag atgagcgcct gccaggtctc gccgtttgac ggggtttggg
     3781 cgcgggcggc cgcggccagc aggcccaggt agcgcctctg gtcggccgtc aggtcggcgg
     3841 cagcgtcggg caccttttcc atgtcaatcg caaagcgcgc cgagtcgggc gcgaagtcct
     3901 ccagccagat ctgcgcggcc gcgatgcgct cgctggccac ggcctgctcg gcggcgtcga
     3961 ggggcgtgcc cttctccgtg tcgagccgcg ccctgatgtc cgtgccggga acctggagga
     4021 gcagcgacag gtggccgaac ggcggccggt actgcgcagc gacggctgcc ggatccgcgg
     4081 cgggatcggc caggctgagg gcgaagatgc gctcagggtc gggcggcagc tcgccgcgtg
     4141 tcggcttgcc ggcgaccgag tcggcgatgc ggtcgaagtc atcgaaaagc cgcggcagcg
     4201 tgtcacccgc tggatcaaag tcgagggcac ggttcgggcg atggcgcagg aaaaggaagc
     4261 gcagctgcgt cggcggtaac agcgccgcga tctcgtgggc ggccgcgccc gtgcccttgg
     4321 acgtgctcat cttcttgccg ccgatgttca ggaactcata tgggatgttg agcggtggct
     4381 cgcgctcgaa gacacgacgc gagatcgcat cagcgcggtc tcttgaaccg ccagctgtcg
     4441 ccagatcctt gccgcagccc tcgatcgtgg cgccgaccag accccagcgc gccgcccagt
     4501 cgacgttcca gacgagcttg gcatggccgc cgaacggcgt gacacggccc gagaaaccgc
     4561 agccgcgcgc ccacgtgacg aggtccggcc ggcattcgta ggcgacggtc gatccgtccc
     4621 agtcgcgcgg cgccgtcgtg ccgattttgc cgcagttctc acagatgaca ctgatcggca
     4681 gccagccatt gggatggttg accgtcgaaa cggtgcggta gatgtcgagg atgacgtcgg
     4741 catggtccag cgccttgtgg atgtaggggt ccatcgcgcc tgatcggtag ttctcgctca
     4801 tccaatagag cgtcggcttg atgccgaggt cggtgaacgt ctcgaggaag agcgtcacga
     4861 agtggcgcgc gtagttgggc tgggcgctgc cttcgggagc gggcacgttg atgagcggga
     4921 cgcccatgta gcgatcgatc gcgtcaccag agagcatcgc ctgggcgtcc atggggtcga
     4981 ggtcttcgac gccgtagcgg aactcgacct cggtgccccg ttcggcgatc gcgcggcgaa
     5041 gggcgtcgtg caaaacgaca ccgcggaggc tgccgacgtg aacggtgcct gacggcgtct
     5101 tggagtcgtt gatgacctga gggccgaccg cctggccggc tatctcatcg gcccagtagg
     5161 cctgccccgc gcggttcgca gggggcgaag tcatgtcagt ggatcgacag gatcttgtac
     5221 ttagagtccc ctgccgggac gctgaccgtg atctcctcgc ccttcttgcg accaagaagc
     5281 gcgcggccga ccggcgattc gttcgagatg cgaccctcag ccggcgatgc ctcagccgag
     5341 ccgacgatca tgaagctctc cttgccatcc tttgactgga tggtcacggt cgagccgatc
     5401 tggacgtgcg tggacgagcg gttctcctcg atcacgacgg cgttcttgac cagcgcggag
     5461 agcgcctgga tgcggccctc gacgaaggcc tgctcgttct tggcgtcctc gtactcggcg
     5521 ttctcggtga tgtcaccgtg ctctttggcc tcgtggatgc gcgcggcgat ctccgcgcgg
     5581 cgcacgttta ccagctcctc gagctccttg cggatctgct cgagaccttc cttggacagg
     5641 tatgcgggtt tgttgttcat ggtgagatgt ccactagata caaacgcccc ggcgatgccg
     5701 ggggccacgg gcggaattgg atccggggtc cggcggattg taggtccggc cgctttggag
     5761 cgtcaaacag tgcgccgcac cgcatgccgc agcgcgcgtc agcccacgcc gaggctggca
     5821 agttgtacca gccgggcgcg aacccggtcg agcgccttcc tatccgagca ggcgatgaac
     5881 accgtgtcgt cgccggcaat ggagccggca acctccggcc agccagtgcg gtccagtgcc
     5941 gccgcgatcg cgtgcgccga cccgggcacc gcgcggatga tcagcatcag gccggacggc
     6001 cgcacctcga tgggcaggtc acgcaggatc agcgccaggc gttcgtcgcc gctggtctcc
     6061 ggcggaccgg agcgcggcgg tagagagtaa tgacgggtgc cgttgcggct gaccttgacg
     6121 aggcccatct cggccacgtc gcggctcacg gtggcctggg tggcgcggaa accgcgctcg
     6181 cgcagcgcgg ccgcgagctc gtgctgcgta gcgatctcgc gcgtgcccag caggtcgcgt
     6241 atctgctgct ggcgcaggcg cttcatcact tccatggtca gtcggcttcc atcggttcag
     6301 ccaattgtga accgcgtctt ggtaacccgc gcacgcacgc aggtcagcgt gccagcagca
     6361 gcaccgcgaa gacgatcgcc gcgcccacgc gatagatccc gaaaaggtca gtgctggcgc
     6421 ggcgcaggta ggcgagcaga aaccagatcg cgatcaagcc tgagatcgcg gccgcaatca
     6481 gtcccgccgc gagcgcggca ccattgaact caaccgtgct gccgccgacc agctcgcgca
     6541 gcttccacag gcccgcgccc gcgatgatgg gtgtcgccat caggaacgag aagcgcgcgg
     6601 cggcgtcgcg cttcaggccc aagatcagtc ccgcgctgat cgtgattcct gaccggctga
     6661 tgcccgggaa cagcgcaaac gcctgcgcaa ggccgatgcc cagtgcctcg accggccgga
     6721 gctcgtccat ctcacgcgtg tggcgtgcaa tccgttcagc cacgaagagc accacggcac
     6781 cgaccacgag gagcgcgcac acgacgagca gctgttcgcg gaagaaggtg tcgacgaagc
     6841 cttccaggcc cacgcccagg agcgcgcccg gaatgatgct gatcgcgagc agcagcgcga
     6901 ccttcctgtc ggcgtcgccc gcgatcgagc ggtcgcgcaa catgcgcagg aaggcgacaa
     6961 agtagcgcca caggtcgcgc cagaaatacg cgatcagtgc gacaactgtc cccaggtgga
     7021 gcgtcgcgtc aaaggccggg ctcaggatga acgcgttgtc ccagcccagg acgcgcggca
     7081 ccaggatgag atgggccgac gacgacaccg gcaggaactc cgtgagcccc tgcacgatgc
     7141 cgacgacgat cgcttgaaag atcacgtcca tctatgtggg cgccccggaa cgccgcgacc
     7201 cgttagcgga tgaacagtgg ggcgagaacg agcgtgatcg tggcgaggag cttcacgagg
     7261 acgtggagcg acgggccggc ggtgtccttg aagggatcgc ccaccgtgtc gccgaccacg
     7321 gctgctgcat gggccggcgt gcctttgcca aggactttgc cgtcggcatc gagcaggtgg
     7381 cccgactcga tgtacttctt ggcattgtcc caggcaccgc cgccgttgtt gagaaccgtc
     7441 gcaagcagga cgccggcgat ggtgccgacc atcagcatgc cggccacggc ctcagcgccc
     7501 aggagcacac ccacgaggat cggtgtcgcc accgcgacga cgcccggcag caccatctgg
     7561 cgcagcgcgg cccgagtcgt gatgtcgacg acccgcgcgt agtcggggcg ctgggtgtag
     7621 tccatgatcc cgggcatctc gcggaactga cgccgcacct cgacgatgat cgactgcgcc
     7681 gtcacgccaa ctgcgcggat ggcgagcgag ctgaagaagt agacgagcat cgcgccgatc
     7741 agggcggcga tgaagaccga cacgtcggcc aggttgaccg aggtgatcag ctcaccgcca
     7801 cggttggtct ggatcaggtt gaccttcgag atgtacgcgg agaacagcag gaacgctgcc
     7861 agtgaggcgg acgcgatcgc gtagcccttg gtcagtgcct tggtggtgtt gcccaccgca
     7921 tcgagtcggt cggtgatctc gcgcgcgctg gcctccgagc ggctgaactc cgcaattcca
     7981 ccggcgttgt cggtaatcgg gccgaacgtg tccatggcca ggatgtaggc cgtggtcatg
     8041 agcatgccca tcgtggcgac ggcggtgccg aagatgccgc cgatggcagc gctctgggga
     8101 tcgttggcga acgcctgcga cccgagccaa tggctggcta ggagggcgat gccgatcgag
     8161 atagccgtca ccgcagtggt ctcgtagccg accgcggtgc cggagatgat gttggtcgcc
     8221 gggccggtct tggacgcctc ggcaatctcg cgcacggggc ggaacgagcc agcggtgtag
     8281 tactgggtga tgtagacgaa ggcaacgctc gtggccaggc caacgacgcc gcagccgaag
     8341 aaccagaccc acgtcgggat gcccatggca cccgcgttta cggggcccgt gctcatcatc
     8401 acgtacgtca cgaaggcgag cgcggccacc gacaggatgg ttgtgaccca atacccacgg
     8461 ttcagcatgt tcatggggtt ctcgtcctcg cggccgcgga cgaagaagat ggccacgatg
     8521 gtggccagca ggccgaaggc gcgcacgaca agcgggaaga agatccaggc ttcagggttg
     8581 ggccaattgg cgatgctcgc gatcgcgaaa acgccgacgc ccaagatcat cgcgccgatg
     8641 ttctcggcgg cggtcgactc gaacaggtcc gctccgcggc cggcgcagtc tccgacgttg
     8701 tcgcccacga ggtccgcgac gacacctgcg ttgcgcgggt catcctcggg aatgcccgcc
     8761 tccactttgc cgacgaggtc ggagccgacg tcggcggcct tggtgtagat accgccgccc
     8821 agctgggcga agagagcgac gaaggaggcg ccgaagccaa agccgacgat caggaagggc
     8881 gcttcggcaa cgctgacctc accgacgagc gattcatagg cggtaaagat gccccacacg
     8941 ccaagcaggc tgagcgcgac caccagaaag ccggagacgg caccgccgcg catcgcgacc
     9001 tgcacggcct cgacgagact gcgtcgcgct gcggcagctg tacgcacgtt cgatttgacg
     9061 ctgatgaaca tgccgatgat ccccgacgcc atcgagcagg cggcgccaac gaggaaagcg
     9121 atggcggtca tgatcgcgat gggagcgccc gccagcgagg gcacgtcagc gacatcgggg
     9181 gtctctacga ggtagatgac gatgccgatg atgaccgcgc cgccaagagc gagaagccca
     9241 atcgtggtgt actggcgcct gatgaaagcg acggcaccct catagatcgt gctcgcaacg
     9301 tcacgcatcg cgtcggttcc ggtgtcgcgg cgcagcacgt cgcgcgccag gtagccggca
     9361 aagagaacgg ccgcgatgcc ggcgatcggg atgatccact gaagcgcgct ggcgagtacc
     9421 acgctgagaa ggcctcctgc tggttgatga gcgcggcgcg aagttgggca gcgcctgcgt
     9481 ctgtgagccc gtagtgggac ttgagaaccg cgccgacacg agcctcggtg ccgcgtcgga
     9541 aagcggcggg agtgtaccag agcaggccac gcccgcgact cccggtgacc gcggctacac
     9601 tcccccgcga gtgagtcgac gtcccctggg atgcctgatc gagatcgccg agaccctcgt
     9661 cctgacgatc atcatcttct ggcttatcca gaccttcgta gcccagccat atcgggtcga
     9721 gcaggagagc atgcgctcca cgctcgagcc gaatcagtac gtgctcgtcg acaagctctc
     9781 gccccacttc gactcgtact cgcgcggcga catcgtggtc ttcaacccgg tcctgcgcga
     9841 cgagtgctcg gccgacgtcg tcggcggcct gacagatgtg accccttaca tcaagcgcgt
     9901 catcggtgag ccgggcgacc gcgtcgagct gctcgacggc ggagtgacgg tcaacggtga
     9961 gccgctcgac gagccgtatg tccacggcgc gcacaccacc ccgctgtcgg gcgagaccgc
    10021 ctggaacgtg cccgccgacc ggttgttcgt gatgggcgac aaccgccaga actcgaccga
    10081 ctcgcgctcc gatcagatcg gcatgatctg cacgcgtgat gtcatcggcc gggcgtggct
    10141 gcgctactgg ccgatagaca cgctgggcat catgcagaca ccgacctacc cgaacgtgcc
    10201 ggctgcctct cccctaccga ccgcctcgcc cgccccctag ccccgcgcgc ggagcaagtc
    10261 ttcggtcagt cgaggaccca gcggtcgatg tcacgcgggt agtcgatcag ctcgtcggct
    10321 tcgaaccaca gctcaagctc agcggccgca ttctccggcg agtccgacgc gtggaccagg
    10381 ttgtggccgg tgtcgaccgc ccagtcgccg cggatggagc cgggtgccgc ctcgtgcggg
    10441 cgcgtcttgc cgttgatctc gcggcacacg gcgatcgcgt tcggtccctc gaccgccatc
    10501 gcaaccagcg gggcggacgt gatgaactcg accagcccgg cgtagaaggg cttttcgcga
    10561 tgtaccgcgt agtggcgttc ggcgagacgg cggtcggcgt ggaccatctt catgccacag
    10621 atgcgcagtc cgcgcgcctc gtagcggtcg acgatcttgc cgatcagttg gcgctgaacg
    10681 gcgtcgggct tgatcaggat cagggttcgt tcagccatgt ggtttcctct cagttcagat
    10741 gcaggacgcg ctcgacagcc tttgcggtac cgcgcggcga gatgacggcc atgcacaggc
    10801 gatcgtcggt gaagagccgc tgggcgagcg actgaattcc ctccggcgtg accttgtcga
    10861 gctcggcgag cgcctggtca agtgtcagga cgcgctcgtg caatgcctcc tggatgccca
    10921 gccaggcaga cagatggcgg ctctcctcga ggcgcagctc cagccgcccg ctcgcgtacg
    10981 cccgcgcgcg atcgagctcg tccatcgcga cgggctcatc gaccatccgg cgcagctcgg
    11041 ccaggatggc ctcggtggcc gattcaaggt cggccggatc gacaccggcg taaatctgca
    11101 atgtcccgca gtcggcgaag tcggtctgga acgagtgcac gtcgtacgcc aggccggcct
    11161 cctcgcgcag ctcctgaaat aggcgtgacg acgagccttc gcccaggatc gtgttgagca
    11221 tctcgagcgt ccactgctcg ggatcgtcgc ggcgcagcgc gggcacgccc agcaccaggt
    11281 gcgcctgggc aagatcgcgt tgctcaacgc tcaagcgctc caccgggagc gccggcacgc
    11341 cgctgaaacc gcggacgacg ccgttgccgg tgccgacgtt gcgcttcacg agatcgacaa
    11401 tcgtggcgtg gtcgaggtcg cccgcgaccg cgacgaccgt attcgccggg cgatatgtct
    11461 ccgaccagaa gcccttgatc tggtccacgg tcaggccgcg cacgctggcc tcgtcgccgg
    11521 cgatctccca gcccagcggc gtatcgccaa agaaagcgcg gtcgaactgg ttgaaaacgt
    11581 gctggccggg gtcgtcctgg tacgagcgga tctcctcgac gatgatgtcg cgctcgcgcg
    11641 cgatgtcggc ctcgcgcagg agcggcctga agaccagctc ggacaggaca ctgaacgcgc
    11701 gctcggcctc gcggaccggc aggcgcgccc agtagatggt cgactcgcgg tccgtcgccg
    11761 cgttcgatga gccgccgcaa ccctcgattg cctcgctcac ggcgcgtgtc gttgggtacg
    11821 ccgtcgtgcc cttgaacgtg acgtgctcca tgaagtgggc gacgccggcg tgctcgcgcg
    11881 tctcgagccg ggagccagcg agaacgtaga tcgcgaccga cagcgagcgg ctgcccggca
    11941 gctgcgcgga gatgacgcgc ggcccattgg gcagcgccgt ccgctcgaac ggggcctcgt
    12001 cagtcatcgg gccggaatgc taca
//
LOCUS       DATBRX010000226         7107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2664848, whole genome shotgun sequence.
ACCESSION   DATBRX010000226 DATBRX010000000
VERSION     DATBRX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2664848"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VIF08_08655"
     CDS             <1..1375
                     /locus_tag="VIF08_08655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY8136093.1"
                     /translation="TLARGLLGGKIKEHKLNNNPLLALEPGETVELGAFSATAFRIGH
                     SIPDAMGIALRTPVGLVVHTGDFKFDYTPVDGKMSDLHALAGFGREGVLLLLSDSTRA
                     ESPGYTPSERIVGEVFRDIMEGLDGRVIVATFASNIARVQQGFDAAATFDRQVTVIGR
                     SMEQNVRIATDLGYLKYPAGQLVQKDRLKEVPDAKLVIATTGAQGEPMAGLARMANRD
                     HRWVEIKPGDTIIVSASPIPGNEEVVGRTIDNLFKVGANVYYHDIKRAHVSGHASQEE
                     LKLMLNLTRPKHFLPMHGEFRMLVQHGRLAAQMGVAPDNIFIIENGQPIEFSADGSAR
                     RGTPVEAGYVYVDGLSVGEVGDVVLRDRRDLASDGMFLVVVTVDKQTGNIIGKPEIVT
                     HGFVAGPDDPVIAAAADRVRSAILNPGDHISEVALLKAQIKDSLSQYLYERTKRRPMV
                     FPVVVEV"
     gene            1386..3734
                     /locus_tag="VIF08_08660"
     CDS             1386..3734
                     /locus_tag="VIF08_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY8136094.1"
                     /translation="MATRTRRSSSRSRARSQSTSGQLWQSLQLPPHVARSLFGLVLIV
                     VGAVTLIALLFPTAILNRYVADALLPLFGQGAWLLAILLIVAGVAVERPGTLGYGSSL
                     TILGGLIVFVAGLGMIHLLWGHGSRDVDLRNGGGALGAALSSGLTDLLSWPGAFVVLL
                     GLLVAGVLLLLNITLRTLVMPVAGGGKMLAKAMATPARALAEGASTRRAEAAANTRGG
                     TPAATRPRGREDKPERPAKPERAPREAPVRPEPLAPEPQSAPLSQTIWSAQLNGASAI
                     AAEPAARAADETDDSQAPARTWTMPTYDLLDTEGISGGPGQRLDHARNIRIIEEKLSS
                     FQIPASVTATNTGPVVTQYEVKPDAAVKLSRIEALADDLAMALAARSIRIEAPIPGRD
                     VVGIEIPNHNSEIVGFKRLLEDAGMSDATSKLTFALGRDVSGKAYAVDLGRMPHLLIA
                     GATGSGKSVCVNALIGSLLMRATPDEVRLILVDLKRVELAAYRDLPHLETPVIVESHE
                     ARAVLTNLVHEMERRYELLAEAMERNLTAFNSSPRRIGKPMPYMVVVIDELADLILRE
                     GRKVEDPIVKIAQKARAVGIHLVLATQRPSVNVVTGLIKANVPSRIAFAMASNVDSRT
                     VLDANGAEDLIGRGDMLYQPADLPRPVRLQGVFVSDPEVRAVADFWRDQAEPNYNRDL
                     LAGADVEDGGENGQFAFLKGTDDELVPRAAELVMQTGRASTSMLQTKLKVGFNRATRI
                     IDELEKHGIIGPLDTRNPGAPRTVYGPDNWIRDAAEANADLD"
     gene            3816..5243
                     /locus_tag="VIF08_08665"
     CDS             3816..5243
                     /locus_tag="VIF08_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEY8136095.1"
                     /translation="MSARQQLRLGNRRLGRDRNPVETTLGPPVGETLQLARERKGVDL
                     YRAERDTKIRLRYLSALEDGDYAELPAPVYTKGFLRNYAIYLGLEPDDILERWREEME
                     QQRTATRVAVIPPPMPIAEPGGRRLHLTPNMVVAGLVGVVTILFIGYIGIQLLRFAAV
                     TPLSLTNPPDVIWQTADASITLAGTTSPGARVHITGPGDQLYDPVASAAGGWQQEVNL
                     AHGENTFEIVAIDRDTQRESTKLVVTINVPLPLSSPGASATPSAGPQIELSLSLSSPI
                     DGQVITDGRVNVTGTTTGNRIRITSTYLGTPDSTPAPSDAPSPTPARSAGPTSTPNIP
                     PAEEVTVGSSGTFNETLEFTPGRWQVTVISYASDQVPVARQVTIIVEEAGPVTHHLIL
                     TVANGPTYIKVLADGSRVANGTLAAGTTREFTATNQWCVRTDNAGGLSMVLDGLTLAA
                     LGGDGEEGSWIIKSGLPPVRAPRPC"
     gene            5247..5756
                     /locus_tag="VIF08_08670"
     CDS             5247..5756
                     /locus_tag="VIF08_08670"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY8136096.1"
                     /translation="MPEADAADELAPLIGAAEHAGSLLRERHLTLATAESCTGGLIGH
                     LLTESPGSSDYYQGGLISYSDTLKRDELGVDAATLEHHGAVSAQTCIAMAEGARKRYG
                     SDVAIAVTGIAGPTGDTPGKPLGLTYVAVADAAGHEVQRHQWFGDRHRNKVASAAAAL
                     TLLVERLQR"
     gene            5837..>7107
                     /locus_tag="VIF08_08675"
     CDS             5837..>7107
                     /locus_tag="VIF08_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY8136097.1"
                     /translation="MLGAAGAGASAAALLAHRAGAQVSGCDAGGASPYTMALEAAGIE
                     IAWGHDPAHVNLPAGQPPERLAVTKALTAVNPDHPELAAARSLGIPVEPWQQVVADAA
                     ATSRQRLVAVAGTHGKSTTTGWLVDVLVRANRDPSAFVGALLDATLTGGTPSTARWGR
                     GDVFVVEADEYAGNFDPYRPSVIVLLNAEWDHPDVFADADAVYDALATWIRAAGKATL
                     IANVADPGVRQVLERLSGWSGSVVTVGLDVPDADVYGQVEFDQEISPASTLVVNGRFG
                     AGVYGIQLPGKHNAANGLMVLAAANVLGVLGEEPGDGPAIGGFRGVGRRLELKGEVRG
                     VTVLDDYAHHPTAIAKTIQAVRERYPGRRLWAVYEPLTFHRTAAMLEGFADVLATADQ
                     VAIADIWAGRDPDTTITSAAALADAVTGRGGP"
BASE COUNT         1162 a         2517 c         2374 g         1054 t
ORIGIN      
        1 gactcttgca cgcggactac tgggcggcaa gatcaaggag cacaagctca acaacaaccc
       61 gctcctcgcc ctcgaacctg gtgagacggt cgagctgggc gcgttcagtg cgacggcctt
      121 ccgcattggc cactcgatcc ccgacgcgat gggcattgcc ctgcgcacgc cggtcgggct
      181 ggtcgtgcac accggcgact tcaagttcga ctacacgccc gtcgacggca agatgtccga
      241 cctgcacgcc cttgctgggt ttggccgcga aggcgtcctg ctgctgctgt ccgactcgac
      301 gcgcgccgag agcccgggct acacgccgtc cgagcgcatc gtcggcgagg tttttcgcga
      361 catcatggag ggcctcgatg gccgggtgat cgtggccacc ttcgccagca acatcgcccg
      421 tgtccagcag gggttcgacg ccgcggccac gttcgaccgc caggtgacgg tcatcgggcg
      481 ctcgatggag cagaacgtgc gcatcgcgac cgacctcggg tatctcaaat acccggccgg
      541 ccagctggtc cagaaggacc ggctcaagga ggtcccggac gcgaagctgg tcatcgcaac
      601 gaccggcgcc cagggcgaac cgatggccgg cctggcgcga atggccaacc gcgaccaccg
      661 ctgggtggag atcaagcccg gcgacacgat catcgtgagc gccagcccca tcccgggcaa
      721 cgaggaggtc gtcggccgaa cgatcgacaa cctgttcaag gtcggcgcca atgtctacta
      781 ccacgacatc aagcgtgccc atgtgtcagg tcacgccagc caggaagagc tcaagctgat
      841 gctcaacctg acccggccca agcacttcct gcccatgcac ggcgagttcc ggatgctcgt
      901 ccagcacggc cgcctggccg cgcagatggg tgtcgcgccg gacaacatct tcatcatcga
      961 gaacggccag ccgatcgagt tctcggcaga tggttcggcg cgccgcggca ccccggtcga
     1021 ggcgggctac gtgtacgtcg atggcctgag cgtgggcgag gtgggtgacg tggtcctgcg
     1081 cgaccgccgt gacctcgctt ccgatggcat gttcctggtg gtggtcacgg tcgataagca
     1141 gaccggcaac atcatcggca agccggagat cgtgacccac ggcttcgtcg ccggcccgga
     1201 cgatccggtc atcgccgccg ccgctgaccg cgtgcggagc gcgatcctga acccgggtga
     1261 ccacatcagc gaagtcgcgc tgctcaaggc ccagatcaag gactcactga gccagtacct
     1321 gtacgagcgc accaagcgcc ggccgatggt cttcccggtc gtggtcgagg tctagacacc
     1381 gactcatggc cacccgcacc cgccgcagct ccagccgatc acgcgcgcgc agccagagca
     1441 catccggcca gctgtggcag tcgctgcagc tgccgcccca cgtcgcgcgg tcgctcttcg
     1501 gcttggtgct gatcgtcgtt ggcgcggtca cgctcatcgc gctgctcttc ccgaccgcga
     1561 tcctcaaccg ctacgtcgcg gacgcgctac tgccgctctt cgggcagggc gcgtggctgc
     1621 tcgcgatcct gttgatcgtt gccggcgtgg cagtggagcg gcccgggacg ctgggttacg
     1681 gctcgagcct gaccatcctg ggcggcctga tcgtgttcgt ggcgggactc ggcatgatcc
     1741 atctgctctg gggccacggc agccgcgacg tcgatctgcg caacggcggt ggtgcgctgg
     1801 gcgcggcgct gtcgagcggt ctgactgacc tgctttcgtg gccgggcgcg ttcgtggtgc
     1861 tgctcggcct gctggtcgcg ggtgtcctct tgctgctcaa catcaccctg cggacgctcg
     1921 tcatgccggt cgccggtggc gggaagatgc tcgccaaggc catggcgacg cccgcgcgag
     1981 cgctcgccga aggggcgagc acgcggcgcg cggaggcggc tgccaacacg cgtggcggca
     2041 cgccggccgc gaccaggccg cgcggccgcg aagacaagcc cgagcggccg gcgaagccgg
     2101 agcgcgcacc gcgcgaggct cctgtcaggc cggagccgct cgcgccggag ccgcagtccg
     2161 cgccgctcag ccagacgatc tggagcgccc agctcaacgg cgcttccgcc atcgcggccg
     2221 agcccgcggc gcgggcggca gatgagacgg acgactcaca ggcaccggcg cggacgtgga
     2281 ccatgcccac gtacgacctg ctcgacaccg agggcatcag cggcggaccg ggccagaggc
     2341 tcgaccacgc gcgcaacatc cggatcatcg aggagaagct gtcgagcttc cagatcccag
     2401 ccagcgtgac cgcgaccaac accgggccgg tggtgacgca gtacgaggtc aagcccgacg
     2461 cggcggtcaa gctctcgcgc attgaggccc tggccgacga cctggccatg gccctcgccg
     2521 cgcgctcgat caggatcgag gcgcccatcc cgggtcgcga cgtggtcggc atcgagatcc
     2581 cgaatcacaa cagtgagatc gtcggcttca agcggctgct cgaggacgcg ggcatgtcgg
     2641 acgcgacgag caagctgacg ttcgcccttg ggcgggatgt gtcgggcaag gcatacgcgg
     2701 tcgatctcgg acgcatgccc cacctgctga ttgccggcgc caccgggtcg ggcaagagcg
     2761 tgtgcgtgaa cgcgctcatc ggcagcctgc tgatgcgcgc cacgccggac gaggtgcgtc
     2821 tcatcctggt cgatctcaag cgcgtcgagc tcgccgccta tcgcgacctg ccgcaccttg
     2881 agacaccggt catcgtcgaa tcccacgagg cacgcgcggt cctgaccaac ctcgtgcacg
     2941 agatggagcg gcgctacgag ctgctggcgg aggcgatgga gcgcaacctg accgccttca
     3001 attccagccc gcgccggatc ggcaagccaa tgccatacat ggtcgtggtc atcgatgagc
     3061 tggccgacct gatcctgcgc gagggccgca aggtggagga tcccatcgtc aagatcgccc
     3121 agaaggcgcg cgccgtgggc attcacctcg tcctcgctac gcagcgcccc agcgtcaacg
     3181 tggtcaccgg cctcatcaag gccaacgtgc cgtcgcgcat cgctttcgcc atggcgtcca
     3241 acgtggactc gcgcacggtc ctcgacgcaa acggcgctga ggatctgatc ggccgcggcg
     3301 acatgctcta tcagccggcc gacctgccgc ggcccgtgcg gctccagggc gtgttcgtca
     3361 gtgatcccga ggttcgcgcc gtcgccgact tctggcggga ccaggctgag ccgaactaca
     3421 accgcgacct gctcgccgga gccgacgtcg aggacggcgg cgagaacggc cagttcgcct
     3481 tcctgaaggg caccgacgac gagctcgtgc cgcgtgccgc ggagctggtc atgcagaccg
     3541 gccgagcgag cacatcaatg ctccagacca agctcaaagt cggcttcaac cgcgccaccc
     3601 ggataatcga cgagctcgag aagcacggca tcatcgggcc gctcgatacg cgcaatccgg
     3661 gtgcgccgcg aaccgtgtac gggcccgata actggatccg cgacgccgcc gaggccaacg
     3721 ccgacctcga ttgaccctgg ccgcgccccc tcgaagcccc cttgaacggt ggcaacgggg
     3781 gcgcatccgc tgacggcgat agactcaacc cttcgatgag tgcccgtcag cagctgcgcc
     3841 ttggaaaccg tcgtttgggg cgcgaccgca acccggttga gaccacgctg ggcccgcccg
     3901 ttggtgaaac gctgcagctc gcccgcgagc gcaaaggcgt cgacctgtac cgcgccgagc
     3961 gcgacaccaa gatccggctg cgctatctct ccgcgctcga ggacggcgac tacgcggagc
     4021 tgcccgcgcc cgtctacacc aagggcttcc tgcgcaacta cgcgatctac ctcggcctcg
     4081 agcccgacga catcctggag cgttggcgcg aggagatgga gcagcagcgt accgcgacgc
     4141 gcgtcgcggt catcccgccg cccatgccca tcgccgagcc cggcgggcga cgcctccatc
     4201 tgacacccaa catggtcgta gccggtctgg tcggcgtggt gacgatcctc ttcatcggct
     4261 atatcggcat ccagctgctg cgcttcgccg ctgtcacgcc gctctcgctg accaacccgc
     4321 cggacgtcat ctggcagacc gctgacgcgt cgatcaccct cgccgggacg accagccctg
     4381 gcgcgcgagt tcacataact ggcccgggcg accagcttta cgaccccgtc gccagcgccg
     4441 ccggcggatg gcaacaggag gtgaacctgg cgcacggcga gaacacgttc gagatcgtcg
     4501 cgatcgaccg cgacacgcaa cgcgagtcaa ccaagttggt cgtgacgatc aacgtgccgc
     4561 tgccgctgtc atcacctggc gcgtcggcca ccccctccgc ggggccgcag atcgagctgt
     4621 cgctcagtct ctcgagcccg atcgacggcc aggtaatcac cgacgggcga gtgaacgtca
     4681 ccggcacgac cactggcaac cggatccgca tcacgagcac ctaccttggg accccggact
     4741 cgacgcccgc gccctcggac gcgccatcgc ccacgccggc tcggtcagca gggcctacgt
     4801 ccacgccgaa cattccgccg gccgaggagg tcacggtcgg ctcatcgggc actttcaatg
     4861 agacgctcga attcacaccg ggccgctggc aggtgacggt catctcgtac gcgagcgacc
     4921 aggttccagt ggcgcgccag gtcacgatca tcgtcgagga ggccggcccg gtcacccacc
     4981 acctgatcct gacggtcgcg aacggcccga cgtacatcaa ggtgctcgcc gatggctcac
     5041 gggttgccaa cgggaccctt gccgccggca caacacgcga gttcaccgcg accaaccagt
     5101 ggtgcgttcg caccgacaac gccggcgggc tgagcatggt gctcgacggc ctgacgctgg
     5161 ccgcgctcgg cggcgacggc gaagagggca gctggatcat caagtccggc ctccctcccg
     5221 tgcgcgcgcc gcgtccctgc tgagcggtgc ctgaagccga cgcggccgac gagctggcgc
     5281 cgctgatcgg tgccgctgag catgcgggca gcctcctgcg cgagcgtcac ctgacgttgg
     5341 cgaccgcgga atcgtgcacc ggcgggctga tcggccatct cctgacggag agccccgggt
     5401 cctccgacta ctaccagggc ggcctgatca gctattcgga cacgctcaag cgcgacgagc
     5461 ttggcgtcga cgccgcgacg ctcgagcatc acggcgcggt atcagcccag acctgcatcg
     5521 ccatggcgga gggcgcgcgc aagcgctacg gatccgacgt ggcaatcgcc gtgaccggca
     5581 tcgccgggcc cactggtgac acccccggca agccgctcgg actgacctac gtcgcagtgg
     5641 cggacgccgc cggtcacgag gtgcagcgcc accagtggtt cggcgatcgg caccgcaaca
     5701 aggtcgcatc ggccgcggcc gcgctgactc tcctggtgga gcggctgcaa cgatgacgcg
     5761 cgcagccgcg gatattgggc aagggcttgc gcccgctcgt cccgcgcggc caatccgagc
     5821 cggcgagcgc attcacgtgc tgggcgcagc cggtgctggc gcgtccgctg ccgcgctcct
     5881 ggctcatcgc gctggcgccc aggtgagcgg ctgtgatgca ggtggtgcga gcccgtacac
     5941 gatggcactc gaggcggcag gcatcgagat cgcatggggc cacgatcctg cgcacgtcaa
     6001 tctgcccgct ggccagccgc ctgagcgact ggccgtcacg aaggcgctga cggcggtcaa
     6061 ccccgaccac cccgagctgg cggccgcgcg cagcctgggc attccagtcg aaccctggca
     6121 gcaggtagtc gccgacgctg cggcaacatc gcgccagcgc ctcgtagccg tggccggcac
     6181 ccacggcaag tcgaccacca ctggttggct ggtcgacgtc ctcgtccggg cgaaccgtga
     6241 tccatcggcc ttcgtcggtg cgctgctcga cgccacgctg accggcggga cgccgagcac
     6301 ggcccgctgg gggcgcggcg acgttttcgt ggtcgaggcc gacgagtacg cgggcaactt
     6361 cgatccgtac cggccatcgg tcatcgtgct gctcaacgcg gagtgggatc accccgacgt
     6421 gttcgccgac gcggacgcgg tctacgacgc gctcgccacc tggatccgcg ccgccggaaa
     6481 ggcgacgctc atcgcgaatg tcgccgatcc cggtgtgcgc caagtgctgg agcgtctgag
     6541 cggttggtcg ggatcagttg ttaccgtcgg gctcgacgtg cccgacgctg acgtgtacgg
     6601 ccaggtcgag tttgatcaag aaatatcgcc agcgtccacg ctggtcgtga acggtcgatt
     6661 cggggccggg gtctatggca ttcaactccc gggcaagcac aacgcggcga acggcctcat
     6721 ggtcctggcc gccgcgaacg ttctgggggt cttgggcgag gagccaggcg atgggcctgc
     6781 cattggcggg ttccgcggtg ttggccggcg gctcgagctc aagggcgagg tgcgcggcgt
     6841 cacggtgctc gacgactacg cccaccatcc cacggccatt gcgaagacca tccaggcagt
     6901 gcgcgagcgg tatccgggga gacggctctg ggcggtctat gagccgctca ccttccatcg
     6961 caccgcggcc atgctcgagg gcttcgccga tgtcctggcc acggccgacc aggttgcgat
     7021 tgccgatatc tgggcgggcc gcgacccgga caccacgatc acctccgccg cggcccttgc
     7081 ggacgcggtc acgggccgcg gcgggcc
//
LOCUS       DATBRX010000227         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1437264, whole genome shotgun sequence.
ACCESSION   DATBRX010000227 DATBRX010000000
VERSION     DATBRX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437264"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VIF08_08680"
     CDS             complement(<1..826)
                     /locus_tag="VIF08_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY8136098.1"
                     /translation="MDRSLLSRRVEVTSPDTGPVRAGEDLEWTRLEAWLRDQRDLPKL
                     DDREPMHVAQFPGGHSNLTYQLRFGATELVIRRPPFGPVPPTAHDMAREYRWLSAIHP
                     VFPLAPRVYALCEDPSVIGSVFYVMERRHGLVVRDQEPPAIRDLPQVRRGVSIAVVDA
                     LADLHAIDIAAAGLAHLGKPHGFVERQVRGWTERWNRSRTSDVPEMEAVARWLVESLP
                     AGPARPTIVHGDFKLDNLLLDPANPCRLVAVFDWEMAALGDPLVDLGILLAYWAATA"
     gene            complement(790..1254)
                     /locus_tag="VIF08_08685"
     CDS             complement(790..1254)
                     /locus_tag="VIF08_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8136099.1"
                     /translation="MTVVDLAHLGGLVGQEVATSDWLAITQDRIDAFADATSDHQWIH
                     VDAERARTETPFGATIAHGFLTLSLLSALVRDAVTVNGPRMTLNYGLNRVRFVSPVPS
                     GSRIRARLALATIADMEDSIQATWSVTVEREGGDKPAIVAEWIVRYYPGASK"
     gene            complement(1251..>1541)
                     /locus_tag="VIF08_08690"
     CDS             complement(1251..>1541)
                     /locus_tag="VIF08_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8136100.1"
                     /translation="DEEIIARIMTALANEGAQVLEDGYATRASDIDVVYVHGFGFPRH
                     RGGPMFYADTVGLPTVLARVNKYREQFGDYWRPSPLLEKLAIDRRGFYGDAA"
BASE COUNT          263 a          525 c          511 g          242 t
ORIGIN      
        1 gcgcggtcgc ggcccaatac gccagcagga tcccgaggtc gacgagcgga tcgccgagcg
       61 ccgccatttc ccagtcgaac accgcaacca gccggcaggg attcgccggg tcgagcagca
      121 agttatcgag cttgaagtcg ccgtggacga tcgtcggccg ggcgggaccc gctggcagcg
      181 actcaacgag ccagcgcgcc accgcctcca tttccggcac atcggaggtc ctggatcggt
      241 tccaccgctc cgtccatccc cgcacctgcc gctcgacgaa cccgtgcggt ttgccgagat
      301 gtgcgagacc ggccgccgca atgtcgatcg cgtgcaaatc ggccagcgcg tcgacaaccg
      361 cgatgctgac tccacgccgc acctgcggca ggtccctgat ggctgggggc tcctggtctc
      421 tgacgacgag gccatgccgc cgttccatca cgtagaacac cgaaccaatg accgacggat
      481 cctcgcaaag ggcgtagacg cgcggcgcca gcggaaagac gggatggatg gctgacagcc
      541 aacgatactc gcgagccatg tcgtgcgcgg tcggagggac cggcccgaac ggcggtctcc
      601 ggatgacgag ctccgtggcg ccgaagcgca actgatacgt caggttcgag tgccctccag
      661 ggaactgtgc gacgtgcatg ggctcgcgat cgtcgagctt cggcagatcc cgctggtcgc
      721 ggagccacgc ctccaggcgg gtccactcga gatcttcgcc ggcgcgaacc gggccggtgt
      781 cgggggaagt cacttcgacg cgcctggata gtaacgaacg atccattcgg ccacgatggc
      841 cggcttgtcg ccgccttcgc gttcgacggt gacgctccag gtcgcctgga tgctgtcctc
      901 catgtcggca atcgtcgcaa gcgcgagccg cgcgcggatt cgcgacccgg acggcaccgg
      961 cgacacgaac cgcacgcggt tcagtccgta gttgagggtc atgcgggggc cgttgaccgt
     1021 gaccgcgtcg cgcaccaacg cgctgagcag cgacagcgtc aggaatccgt gcgcaatcgt
     1081 cgcgccgaag ggagtctcgg tccgcgcccg ctcggcgtcg acatggatcc attgatggtc
     1141 gctcgtcgca tccgcaaagg cgtcgatgcg gtcctgcgtg atcgcgagcc agtcgctggt
     1201 cgcgacctcc tgcccgacca gcccgccgag gtgcgccaga tcgacgacgg tcacgccgcg
     1261 tcaccgtaga agccacggcg gtcgatcgcc agtttttcga gcaatggcga cggccgccag
     1321 taatcgccga actgctcgcg atacttgttg actcgcgcca gaacggtcgg cagcccgacg
     1381 gtgtcggcgt agaacatcgg gccgccgcgg tggcggggaa agccgaaccc atgcacgtac
     1441 acgacgtcga tgtcactggc tcgcgtggcg tatccgtcct ccaggacctg ggcgccttcg
     1501 ttggcgagcg ccgtcatgat ccgcgcgatg atttcctcgt c
//
LOCUS       DATBRX010000228         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3229473, whole genome shotgun sequence.
ACCESSION   DATBRX010000228 DATBRX010000000
VERSION     DATBRX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229473"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VIF08_08695"
     CDS             <1..751
                     /locus_tag="VIF08_08695"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8136101.1"
                     /translation="APPIGAFLFAIGMALPFATNAAAFLLGAVLISRISASVGDVAAA
                     RSGERAHFVADLVEGVRWLAGHPAMRTLAITIFAFNVTYGAAMGVLVLYAAQRLHMDA
                     VGFGLLTTAAAAGGVVATVSYVALTRRFSLGNIMRVGLVVETLTHLTLAMTTSPIVAL
                     AIMFASGVHGFTWGTTATTVRQRVVPNELLGRVGGVMRFAGYGGLVIGTPIGGLIAGQ
                     YGITAPFWVGFVGSAILVVLLWRQFAHIAHD"
     gene            761..2431
                     /locus_tag="VIF08_08700"
     CDS             761..2431
                     /locus_tag="VIF08_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY8136102.1"
                     /translation="MAAPDLIQLIRDSLIGADEAVAGPFGLRRVTYADYTASGRSLSF
                     IEDYLRDAVMPLYANTHTESSGTGLQTTRFREDARRIVREALGGTEKDHALVFCGAGS
                     TGAVDKLVAILNLRLPADLDDRYHLADQIPAAERPVVFIGPFEHHSNELPWRESIADL
                     VTIHEDADGHIDLAELEAKLVAYADRPLKIGSFSAASNVTGIVSDTRAISALLHRHGA
                     LSFWDFAAAAPYVDISMEGKDAIFLSPHKFIGGPGTPGVLLIRRELMTNRVPAVVGGG
                     TVAYVNSDTHVYLTDKEHREEGGTPAILESIRCGLVFQLKSAVGVATIREREESFIRR
                     AIERWEKHPNIEILGNRQAERLSIVSFVVKHGTSGQHLHHNFVVAVLSDLFGIQSRGG
                     CSCAGPYGHRLLGIDYETSLRYESETRKGCDGIKPGWVRVNFNYFIEEEVFDFILQAV
                     EIVASDGWKLLPQYRFTPETGLWRHASGGVEAPLSLNDITYDSGLMTYQAHRHREPAS
                     RLADYLAEARSILAGSPRTDLPIDHPDLDADFELLRWFPLPSEGVAAP"
BASE COUNT          402 a          922 c          840 g          441 t
ORIGIN      
        1 cgcgccaccg atcggcgcgt tcctgttcgc gatcgggatg gcgttgccct tcgcgacgaa
       61 tgccgcggca ttcctgctgg gagcggtgct gatctcgcgc atctcggcga gcgtcggcga
      121 cgtcgcggcc gcccgttccg gcgagcgagc gcatttcgtc gcggacctcg ttgaaggcgt
      181 gcgctggctt gccggccatc cggcgatgcg caccctggcg atcacgatct tcgcgttcaa
      241 cgtcacgtac ggcgcggcca tgggcgtgct cgtcctctac gccgctcagc ggctacacat
      301 ggacgcggtg ggcttcggcc tgctcaccac ggccgcggcg gccggcggcg tcgtcgcgac
      361 cgtctcgtac gtcgccctca cgcggcgttt cagcctgggc aacatcatgc gcgtcggcct
      421 ggtggtcgag accctgaccc acctgacgct cgcgatgacg acgtcaccga tcgtcgccct
      481 ggcgatcatg ttcgcgtctg gcgttcatgg cttcacctgg ggcacgaccg cgaccaccgt
      541 ccgccagcgc gtcgtgccga acgagctgct gggccgagtg ggtggtgtga tgcgtttcgc
      601 tggctacggc ggcctcgtca tcggcacgcc gatcggtggg ctgatcgccg gccagtacgg
      661 gattaccgcg ccgttctggg tcggcttcgt gggctcggcg atcctcgtgg tgctgctatg
      721 gcgccagttc gcacacatcg cccacgacta ggatgcgcga atggccgcgc ctgacctgat
      781 ccagctcatc cgcgactcgc tgatcggcgc ggacgaggcg gtagcgggac cgttcggcct
      841 gcggcgagtc acctacgccg actacaccgc gtccgggcgg tcgctgagct tcatcgagga
      901 ctacctgcgc gacgcggtga tgccgctcta cgcgaacacg cacacggagt cgagcggcac
      961 ggggctccag accacgcgtt tccgcgagga cgcgcggcgc atcgtccgcg aggcgctagg
     1021 cggcacggag aaagaccacg ccctggtctt ttgcggcgct ggctcgactg gcgccgtgga
     1081 caagctagtc gcgatcctga acctccgatt acccgcggac ctggacgacc gctaccacct
     1141 ggcagaccag atcccggcag cggagcggcc cgtcgtgttc atcggcccgt tcgagcatca
     1201 cagcaatgaa ctgccgtggc gcgagtcgat cgccgacctc gtcacgatcc acgaggatgc
     1261 cgacgggcac atcgacctgg cggagctgga ggccaagctg gtcgcctacg ccgaccgacc
     1321 gctcaagatc ggctccttct ccgcagcttc gaacgtgacc ggaatcgtca gcgacacacg
     1381 tgcgatctcg gcgctgttgc atcgccacgg cgcgctgtcg ttctgggact tcgcggcggc
     1441 cgcgccgtac gtcgacatct cgatggaagg caaggacgcg atcttcctct cgccgcacaa
     1501 gttcatcggc ggcccaggca cacccggcgt cctgctgatc cggcgcgagt tgatgaccaa
     1561 tcgcgtcccc gcggtcgtcg ggggcgggac cgtcgcttac gtcaacagcg acacccacgt
     1621 ttacttgacc gacaaggagc atcgcgagga gggcggcacc ccggccatcc tcgagtccat
     1681 tcgatgcggg ctcgtcttcc agctcaagtc ggccgtcggc gtcgcgacca ttcgcgaacg
     1741 cgaggagtcc ttcatccgcc gcgccatcga gcgctgggag aagcacccca acatcgagat
     1801 cctgggcaac cgccaggccg agcggctgtc gatcgtgtcg ttcgtcgtga agcacggcac
     1861 gagcggccag catctccacc acaacttcgt cgtggcggtc ctgagtgact tgttcggcat
     1921 ccagtcgcgc ggcggctgtt cgtgcgccgg cccgtacggc catcgccttc tcggcatcga
     1981 ctacgagacg tcgctccgtt acgagagcga gacgcgcaag ggctgcgacg gcatcaagcc
     2041 cggctgggtg cgcgtgaact tcaactattt catcgaggaa gaggtcttcg acttcatcct
     2101 ccaagcggtc gagatcgtcg cgtcggacgg ctggaagctg ctcccgcaat accgcttcac
     2161 ccccgagacc ggcctgtggc gccacgccag cgggggagtg gaggcaccgc tctcgctgaa
     2221 cgacatcacg tacgactccg gcctcatgac ctaccaggcg caccgccacc gcgagcccgc
     2281 atcccgcctg gccgattacc tcgccgaggc ccgatcgata ctggccggct cgccacgcac
     2341 ggacctgccg atcgaccacc ccgacctgga cgccgacttc gagctcctgc gctggttccc
     2401 tctaccgagc gagggcgtcg cggctccgta gtcgtgtaat gtcgggctgc cagaccctgg
     2461 cggcccaagc tagagggaac cacatgcgcg ggcatcatgc gttcgctgtc gttgtcgtcg
     2521 tcggcctgat cggatgctcg agcgctcaac cgagtggcag cccgtcgagc cagccgtcga
     2581 acagcgccgc acccaccgcc accgc
//
LOCUS       DATBRX010000229         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2449106, whole genome shotgun sequence.
ACCESSION   DATBRX010000229 DATBRX010000000
VERSION     DATBRX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449106"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2207)
                     /gene="metH"
                     /locus_tag="VIF08_08705"
     CDS             complement(<1..2207)
                     /gene="metH"
                     /locus_tag="VIF08_08705"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY8136103.1"
                     /translation="MKRRDAGEVERRTSVLKDLLRARILVLDGAMGTLIQSHGLDEAG
                     FRGDRFANHPRDLRGANEVLNLVRPDIVRAIHAAYLDAGADVVSTNTFNATRISMAEY
                     GLESLVEEINRAAAAIAREAADEATTRDPGKPRFVAGSLGPTNRTASISPDVNDPGAR
                     NVTWDELVAAYMEAGRGLVAGGADILLIETIFDTLNGKAAIFAVEALFDELGFRLPVI
                     VSGTITDQSGRMLTGQTVGAFWNSVRHARPLAVGLNCALGARMLRPYVAELARIADVP
                     VVTYPNAGLPDGFGGYHDNLPETASVLGQLAQEGGLNLVGSCCGTTPDHTRAIAAAVA
                     GVTPRQVPVVEPRTRLSGLEALDLGPDSLFANVGERTNVTGSRAFARLIKEDRMDDAV
                     AIARQQVDAGAQLIDVNMDEALLDSEATMARFLNLIAVEPDIAKVPVVIDSSKWTVIE
                     EGLKRVQGRPIVNSLSLKEGEGEFLRQARLARRYGAAVIVMAFDEQGQADTVERKIAI
                     GKRARQLLIDQAGFDPTDVILDPNIFAVGTGIEEHAGYGVAYLDAVRRIKKELPGTLV
                     SGGVSNLSFSFRGNDAVREAIHSVFLYHAIKAGMDMGIVNAGALPVYDDIEPRLRDAA
                     EDLVLNRRPDATERMLEIATELAGSGGAGGAIAGGRDLAWRDAPVNERLRHSLIEGIA
                     DYIVDDTEEARHAAARPLDVIEGPLMAGMDAVGDLFGSGRMFLPQVVKSARVM"
     gene            2536..3219
                     /locus_tag="VIF08_08710"
     CDS             2536..3219
                     /locus_tag="VIF08_08710"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8136104.1"
                     /translation="MKRPLGVRNQQTARPQALAKLALLGVSMLAGPSRQARFRNEGGD
                     AQVVAAGEPSAFDLNAKRSSLRSRLLTGLLLAVGLTVLAGGIAIRVADLHLAPVLSNS
                     MQPAFSAGDLVITQTVPISSIRVGDVITFVPPGGGRSVIHRITSMQDGVVTTRGDANS
                     TDDPWRATLAGPTTDRLVAVLPYAGWLTELQRPALLLAGLLAGLAFVIALWKGVKSRF
                     LRFQSQPKA"
BASE COUNT          542 a         1158 c         1100 g          572 t
ORIGIN      
        1 ttcatcaccc gcgcgctctt gacgacctgc ggcaggaaca tgcgccccga tccgaacagg
       61 tcgcccaccg cgtccatgcc tgccatgagc ggcccctcga tgacgtcgag cggccgtgcg
      121 gcagcgtggc gcgcctcttc cgtgtcatcg acgatgtagt cagcgatacc ctcgatcagg
      181 gagtgacgta gccgctcatt gaccggcgcg tcgcgccagg ccaggtcgcg cccgccggcg
      241 atcgcgccgc ccgctccgcc gctgcctgcg agctcagtcg caatctcgag catgcgctcg
      301 gtcgcgtccg gccggcggtt gaggacgaga tcctcggcgg catcgcgcag gcgcggctcg
      361 atgtcgtcgt acaccggcag cgcgccggcg ttgacgatgc ccatgtccat cccggccttg
      421 atcgcgtggt acaggaagac gctgtggatc gcctcgcgca ccgcgtcgtt gccgcggaac
      481 gaaaacgaga ggttggacac gccgccgctg accaacgtcc cgggcaattc cttcttgatg
      541 cgccgcaccg cgtctaggta ggccacgccg tagccggcgt gctcttctat gcccgtgccg
      601 acggcgaaga tgttcgggtc gagaatcaca tctgtcggat cgaagccggc ttggtctatc
      661 aaaagctgcc gcgcgcgctt gccgatcgcg atcttgcgct cgacggtgtc ggcctggccc
      721 tgctcgtcga aggccatgac gatcaccgcc gcgccgtacc gtcgcgcaag cctggcctga
      781 cgcaggaact cgccctcgcc ttccttgagc gacagcgaat tgacgatggg ccggccctgg
      841 acgcgcttca ggccttcctc gatgacggtc cacttggagg agtcgatgac caccggcacc
      901 ttggcgatgt ccggctccac ggcgatcagg ttgaggaagc gcgccatcgt cgcctccgag
      961 tcgagcagcg cctcatccat gttgacgtcg atcagctggg cgccggcgtc gacctgctga
     1021 cgcgcgatcg cgaccgcgtc gtccatgcgg tcttccttga tcagccgcgc gaaggcgcgc
     1081 gagccggtga cattcgttcg ctcgccgacg ttcgcgaaga gcgaatccgg gccgaggtcg
     1141 agcgcctcga ggcctgacag gcgcgtccgc ggttccacga ccggcacctg gcgcggggtc
     1201 acaccggcca ccgcagcagc aatggcgcgc gtgtgatcgg gcgtggtgcc gcagcacgag
     1261 ccgacgaggt tcaggccgcc ttcctgggcg agctggccca gcaccgacgc cgtctccggc
     1321 aggttgtcgt ggtagccgcc gaagccgtcg ggcaggccgg cgttgggata agtgaccacc
     1381 ggcacgtcgg cgatgcgcgc gagctccgcg acgtacggcc gcagcatccg cgcgcccagg
     1441 gcgcagttca ggcccaccgc gagcggccgc gcgtggcgga cgctattcca gaaggcgccc
     1501 acggtctggc cggtgagcat ccgacccgac tgatcggtga tggttccgct cacgatgacc
     1561 ggcaggcgga agccgagctc atcgaacagc gcctcaacgg cgaagatcgc cgccttgccg
     1621 ttgagcgtgt cgaagatcgt ctcgatgagc aggatgtcgg caccgcccgc gacgaggccg
     1681 cggcccgcct ccatgtacgc ggcgacaagc tcatcccagg tcacgttgcg tgcgccggga
     1741 tcgttgacgt ccggcgagat cgaggcggtc ctattggtcg ggccaaggga gccggcgacg
     1801 aagcgcggct tgccgggatc ccgcgtggtc gcctcgtccg cggcttcccg cgcaatcgcc
     1861 gccgcggcgc ggttgatctc ctcgactaac gactcgaggc cgtactcggc catcgaaatg
     1921 cgcgtcgcgt tgaaggtgtt cgtgctgacg acgtcggccc cggcgtccag gtaggccgcg
     1981 tggatcgcgc gcacgatgtc cggtcggaca aggttcagga cctcgttcgc gccgcgcagg
     2041 tcgcgcggat ggttcgcgaa ccggtcgccg cggaagccag cctcgtccag gccgtgcgac
     2101 tgaatgagcg tgcccatggc cccgtcgagg accaggatcc gcgcgcgcag caagtccttg
     2161 aggacagatg tgcgccgctc gacctcgccc gcatcacgtc gcttcatctg acgagtctat
     2221 cgacgtggtg gccggtgccc aaaagcgcca gttaggggcc gaacggcata tcaggccgcg
     2281 actttgtgcc tatcgggtaa ccagtactaa aggactatga ctgtctgggt gagttttctc
     2341 gccatggctc tggtcccggg cctccggggc gagagcgcgc taagcgactg ccagcgggtc
     2401 gcaatacaga tagttgaccg cgaccccatc agccgcgatc ggaccgaccg aacgacctag
     2461 acgccgtcgg ccgcgggagg gtgaccaaca acacgtcgcc tgttcaacgt ccccgcgccc
     2521 agagcgcgtt gacagatgaa acgtccgctg ggagtccgta atcaacagac tgcacgtcct
     2581 caagcattgg ccaagctggc gctcctgggc gtaagcatgc ttgctggacc gtcacgccag
     2641 gcccgcttca ggaatgaggg cggcgacgcc caggtcgttg cagcgggcga gccctccgca
     2701 tttgacctga acgccaagcg ttcaagtctt cgctcgcgtc tactcaccgg cctgctactc
     2761 gcggtcggcc tcactgtcct ggcgggcggc atcgctattc gagtggccga cctgcacttg
     2821 gcgcccgtac tcagcaactc catgcagccg gcgttctcag ctggtgatct tgtcataacc
     2881 caaaccgttc cgatcagttc gatccgcgtt ggcgacgtaa tcacgttcgt gccgccgggc
     2941 ggcggccgct ccgtgatcca ccgcatcact tcgatgcagg acggggtcgt gacgacacgg
     3001 ggtgacgcga actcgacaga cgacccctgg cgggcaacgc tcgccggccc aaccaccgac
     3061 cgactggtcg ccgtcctgcc ctatgcgggc tggttgaccg aactacaacg tccagcccta
     3121 ttgctcgccg gcctgctcgc cggcctggcg tttgtgatcg cgctctggaa gggggtgaag
     3181 tcgaggtttt tgcggtttca gtctcagccc aaggcctgac ccggcggatg tcccgccaga
     3241 cacatcgctt acccgaaagg taggaactca atcgtgactc tacgaaagac cgccggagca
     3301 ctggcggcat tcggtctcat cgtcggcctg ctcggcagcg gcgtcgccgc cagcttctcc
     3361 gatttcgtca cc
//
LOCUS       DATBRX010000230         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_549613, whole genome shotgun sequence.
ACCESSION   DATBRX010000230 DATBRX010000000
VERSION     DATBRX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549613"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VIF08_08715"
     CDS             <1..783
                     /locus_tag="VIF08_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY8136105.1"
                     /translation="IERVVIYLAAVLPFMSTLLGGWAVFRLRHRLHVAMAFAAGILVA
                     TALVDLIPESISLIGGADPAAATGIAAIVGFLVYAAVEAFIHQASYEHSHDPDQDPHE
                     PHEHTGPVSAAQSTLGWLAPAGLILHSTLDGVAIGLGYEAGGQVGLLVTLAVLVHDFA
                     DGLNIVTVAFAGGRGRRGAVVLLALDAVATIVGVAISQVIPLDPVQLGLLLGAFGGAF
                     IAIGASHLLPEAQHRQSGSVSPLVGLVVLGAAVVVAVRALIA"
     gene            807..1733
                     /locus_tag="VIF08_08720"
     CDS             807..1733
                     /locus_tag="VIF08_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8136106.1"
                     /translation="MTATSDVISVTDLRRHYGEVRAVDGVSFEVHKGEVFGLLGPNGA
                     GKTTTIEILEGLNRADSGSISVLGFDPKRQADELKERIGVQLQAASLYPDLKVSELVE
                     LFATFYAKRLPAERLITQLGLDERKGALTKELSGGQRQRLSIALALVNDPELIFLDEP
                     TTGLDPQGRRSLWEHIERLRGEGRTVLLTTHYMEEAEQLCDRVAIIDHGKILEIGAVD
                     ELISRHFSERTLRFRSRAEISDSQLASLRDVSHVNHEEDEVVLYTSNVPRTIAALLEL
                     AEAAGADDLDVVVRRPTLEDVFLELTGRALRD"
     gene            1748..>2446
                     /locus_tag="VIF08_08725"
     CDS             1748..>2446
                     /locus_tag="VIF08_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8136107.1"
                     /translation="MRGFLPLTIANIKSFYRNRAALFWTLAFPVIFIVLFGSIFSGGT
                     QNFPLALVDEDHSEVSAQLIAGFDDVSILEAEQVDREEALAHMQSGEFAGVLIIPQGA
                     AASLASGQAVNLTLITDPSRQTATTALQQVVGQVVASMNLRLSNAPTLLTVQPQTMQT
                     QNISNAAYFVPSILAMALMQLGVFASIPLVSQREKQILKRLAATPLSRVTFVGSNVVM
                     RLLIAALQTVTIVGI"
BASE COUNT          375 a          847 c          808 g          416 t
ORIGIN      
        1 atcgagagag tcgtgatcta tctcgccgcg gtcctgccct tcatgtcgac gctccttggc
       61 ggctgggccg tgttccggtt gcgccaccgg ctgcacgtcg cgatggcgtt tgcggctggc
      121 atcctcgtag cgacggcgtt ggtcgatctc atccccgagt cgatcagcct gatcggcggc
      181 gcggaccccg ccgcagcaac gggcattgcc gcgatcgtgg gcttcctcgt ctacgccgcg
      241 gtcgaggcgt tcatccacca ggcgagctac gagcacagcc acgaccccga ccaggacccg
      301 cacgagccgc acgagcacac cgggccggtg agcgcggcac agtcaacctt gggctggctg
      361 gcgccggccg gattgatcct ccattccacg ttggacggcg tggccatcgg cctgggctat
      421 gaggccggtg ggcaggtggg cctgctggtg acgctcgccg tgctagtcca tgacttcgcc
      481 gacgggctga acatcgtgac cgtcgccttc gcgggcgggc gcggccgcag gggcgccgtc
      541 gtgctcctgg ccctcgatgc ggtggcaacg atcgtgggcg tggcgatcag ccaggtaatc
      601 ccgctcgacc ccgtgcagct gggcctcctg ctgggcgcgt tcggcggggc cttcatcgcg
      661 attggcgcga gccacctctt gcctgaggcg cagcaccgcc agagcggctc cgtctcgccg
      721 ctggtcgggc tggtcgtcct gggcgcggcc gttgtcgtcg ccgtgcgcgc gctcatagcg
      781 tgatccgtta gaggacactg ggcagcatga ccgcgaccag cgacgtcatc tccgtcaccg
      841 acttgcgccg ccactacggc gaggtcaggg ccgtcgatgg cgtcagcttc gaggtgcaca
      901 agggcgaggt gttcggcctg ctcggcccca acggcgccgg caagacgacc acgatcgaga
      961 tcctcgaagg cctgaatcgg gccgattcag ggtcgatctc cgtgctgggc ttcgatccca
     1021 agcgccaggc cgacgagctc aaggagcgca tcggcgtgca actccaggcc gcgtcgctct
     1081 acccggacct gaaggtcagc gagctggtcg agctgttcgc tacgttctat gccaagcggc
     1141 tgcccgcgga gcgcttgatc acccagctcg ggctcgacga gcgcaagggc gcgctgacca
     1201 aggagctgag cggcggccag cgccagcggc tgtcaatcgc gctggcgctc gtgaacgacc
     1261 cggagctgat cttcctcgac gagcctacga ccgggctcga tccgcagggc cggcgctctc
     1321 tctgggagca catcgagcgg ctgcgcggcg agggccgcac agtgctgttg acgacgcact
     1381 acatggaaga ggcggagcag ctgtgcgacc gcgtcgcgat catcgaccac ggcaagatcc
     1441 tcgagatcgg cgcggtcgat gagctgatca gtcgccactt cagcgagcgc accctgcgct
     1501 tccgatcacg ggcggagatc tccgattcgc agctggccag cctgcgcgac gtctcacatg
     1561 tcaaccacga ggaggacgag gtcgtgctgt acacgtcgaa cgtgccgcgc acgatcgccg
     1621 cgctgctcga gctcgccgag gcggcaggcg ccgacgatct cgacgtcgtc gttcgccgac
     1681 ccaccctcga ggacgtgttc ctcgagctga ccggccgggc actgcgcgac taggcgagac
     1741 aagaatgatg cgcggcttcc tgcccctgac gatcgccaac atcaagagct tctaccgcaa
     1801 tcgcgcggcg ctcttctgga cgctcgcgtt cccggtgatc ttcatcgtcc tcttcggctc
     1861 gatcttctcc ggtggcacgc agaacttccc gctcgccttg gtcgacgagg accactcgga
     1921 agtgtcggcg cagctgatcg ccgggttcga cgacgtgtcc atcctcgagg ccgaacaggt
     1981 cgaccgtgaa gaggcgctgg cgcacatgca gtcgggcgag ttcgccgggg tcctgatcat
     2041 cccccagggc gcggccgctt ctttggccag cggccaggcg gtgaacctga cgctcatcac
     2101 cgatccatcg cgccagacgg ccacgaccgc gctgcagcag gtcgtcggcc aggtcgtcgc
     2161 gtcgatgaac ctgcggctgt ccaacgcgcc aacgctgctc accgtccagc cgcagacgat
     2221 gcagacgcag aacatctcga atgccgccta cttcgtgccg agcatcctcg ccatggcgct
     2281 gatgcagctc ggcgtgttcg cgtcgatccc gctcgtcagc cagcgcgaga agcagatcct
     2341 caagcggctc gcggccactc ccctgtcgcg cgtcaccttc gtgggctcga acgttgtcat
     2401 gcggctgctc atcgccgcgc tccagacggt gacgatcgtt ggcatc
//
LOCUS       DATBRX010000231         6779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_441838, whole genome shotgun sequence.
ACCESSION   DATBRX010000231 DATBRX010000000
VERSION     DATBRX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441838"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VIF08_08730"
     CDS             <1..727
                     /locus_tag="VIF08_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8136108.1"
                     /translation="AIVDYAYGTEAQPNLPNLEGLIYATNEGAMLAGYASASWSTTKI
                     LSTFGGQQIGPVTDFMDGFVAGANYWNAQKSDTVTVLGWDPVAKTGSFTGNFSSIDDA
                     KGISLGFLQEDADVIMGVGGNIGQGAFEAIREQDKEDSAAALGVDVDWADTHPEDIDL
                     LLTSVLKRLQASVFAAIQRGVNGEPVGEIYLNNLANGGIDLAPFHNFDSAISDSTKTE
                     LDALKAAIIDGSVKVSDYFAPAQ"
     gene            820..2373
                     /locus_tag="VIF08_08735"
     CDS             820..2373
                     /locus_tag="VIF08_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8136109.1"
                     /translation="MKLELRGITKRFPGVLANDDISLRVGDGEILGLLGENGAGKTTL
                     MNILYGLYQPDAGAILIDDKPVRFSGPGDAIAAGIGMVHQHFMLVPVFTVAENVMLGV
                     ESTKTVLGMLDLAAVRQRIREISERHNLRVDPDARVEDLAVGVRQRVEIIKTLYRQSH
                     VLVLDEPTAVLTPQEGEELFTIMRSLVAGGTSIIFITHKLHEVLEVADRIAVLRRGRV
                     VGTTTPAETSAEQLATMMVGREVDLIVDKASAKPGEPVLEVHGLHVRDDRGHLAVNDV
                     DLTVHAGEIVAVAGVQGNGQTELAESITGLRSPELGEIKIGGQPVRLNPRSVYREGVG
                     HVPEDRMEEGLVPAFNVAENLVLNSYWAPPYGHGLALDRDKMASVAEERIKEFDIRTP
                     SVFNPVTNLSGGNQQKVIVAREFSRPLKLLVAVQPTRGLDVGSIEYIHRRLVQKRDEG
                     AAVLLVSTELDEVTALGDRIAVMYQGKIVSMLSPEEATFEKLGLLMGGANPEGHSPEE
                     VLAMAGKAQ"
     gene            2370..3650
                     /locus_tag="VIF08_08740"
     CDS             2370..3650
                     /locus_tag="VIF08_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8136110.1"
                     /translation="MSDELIVPEPAPDADAPAGAVGQPRRNAWSRVAGWLQPLVLPAL
                     ALFSAAVVGAVVIIAGNRDVVGLLERDPAAGLSAAWSIVVESYAALIRGSLGDPAQIA
                     SALAGLNPTALIDAFHPLSESVLSMTPLVLTGLSVALAFRAGLFNIGGEGQLQMGALA
                     AVFVGFSFQGLPWFIHLPLALLAALAGGALWGLIPAVLKARTGAHEVITTIMLNFVAV
                     QLVFFSLRSPLFQRPGRSDPISKVIEPSAELPLLVEGLRAHWGIVLALIAAIFVSWLL
                     FRSTKGFEFRAVGLNPIAARYAGMGIGGTIVASMMIAGALSGLGGGVIVLGTVHNLIP
                     ALGGGAGFDGIAIALLGRSRPAGVVAAAFLMGALRAGSTPMQVATGTPSYLVTVIQAL
                     IIMFVAAPALVRAIYRIRAERTTGTETFAKGWGA"
     gene            3647..4933
                     /locus_tag="VIF08_08745"
     CDS             3647..4933
                     /locus_tag="VIF08_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8136111.1"
                     /translation="MTAASVAAASGRVMSLSERAALRRQRILGFIYLGAAAVIVFAFA
                     LPIGPDVQSKFVLALGPGDIAVPPLILPSTLTLYFLAGLAAFAGAAQLVRGFGSWWIV
                     ALALVAFAFVFSFLTWVAAEGSMNLTGLLQTTVARSVPIIFGALSGILCERSGVINIA
                     IEGMLLSSAFAAAVTASVTGDYWSGILAAMITGALMALLLGVLAIRYQVDQIVAGFVI
                     NILATGLTSYLHQAVLVPNATLNQPPRIDRLPIPLLSDIPIIGPIFFQHNLFVYTAFI
                     LVGVIWFGLFKTRWGLRVRAVGEHPRAADTVGINVNFVRYRNVILGGMVAGIGGAYFT
                     IGSTGTFEREMTGGRGFIGLAAMIFGGWNPVGAAGSGLVFGFADAIQARAAIFKIPVP
                     SEFLLMLPYLVTIIVVAGLVGRVRAPAADGVPYKKD"
     gene            4930..5880
                     /locus_tag="VIF08_08750"
     CDS             4930..5880
                     /locus_tag="VIF08_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY8136112.1"
                     /translation="MNAPVGHGRPVILDCDPGHDDALAMLLALARPEIDLAAITTVAG
                     NSTLDNTTRNALSVLTLVGRTDVPVASGAAAPLQRELITAPNVHGGSGLAGADLPAPA
                     VDVASDDAAAFMAQLVRQSQVPITLVPTGPLTNVAQFQARFPDEFRRLESICLMGGSI
                     TEGNVSASAEFNIWVDPEAARAVFESGLPVTMIPLDVTHQALVSLTDADRMEAFGNRT
                     GIVFADLLRFFALFHIERYGWQGAPIHDAVAVANVALPDMVERRAYRVDVETVSELTR
                     GRTVVDLHGVSRRAPNAQVGIRIDQPRFVEVLLDAVAQFP"
     gene            6085..>6779
                     /locus_tag="VIF08_08755"
     CDS             6085..>6779
                     /locus_tag="VIF08_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8136113.1"
                     /translation="MDLAGDTGGLAHNGGDLQGDLAHTTVKVSPRSVEEAPLVLIIED
                     EETQRTLLARVLEREGYRVIGLADGETGLRAIVEHSPQLVLLDLNLPRMDGYEICRQL
                     RADPMTATLPVMVITAHTAPGDMVAALDAGADDFLAKPVQQYELLARIRSAMRLRRAI
                     TSLERATQIVAALANAVEAKDLNLVHHCRWLAHHAARVGANVGLRGEELEAVAYGALL
                     HDVGKIGVSEHLLR"
BASE COUNT         1081 a         2219 c         2237 g         1242 t
ORIGIN      
        1 cgccatcgtc gactatgcct acggcacaga ggcgcagccc aacctgccca acctcgaagg
       61 cctcatctat gccacgaacg aaggcgccat gcttgctggc tacgcgtctg cttcgtggtc
      121 gacgaccaag atcctgagca cgtttggtgg ccaacagatt gggccagtga ccgacttcat
      181 ggatggtttc gtggccggcg cgaactactg gaacgcgcag aagagcgaca cggtcacggt
      241 tcttggctgg gatcccgttg ccaagacagg gtcgttcacc ggcaacttca gcagcatcga
      301 tgacgcgaag gggatcagcc tcggcttcct ccaggaagat gccgatgtga tcatgggcgt
      361 cggtggcaac atcggtcagg gcgccttcga ggcgatccgc gagcaggaca aggaggactc
      421 cgctgcagcc ctgggtgtgg atgtcgactg ggccgacacc catcccgagg acatcgacct
      481 gctcctgacg agcgtcctga agaggcttca ggcgtctgtt tttgccgcca tccaacgcgg
      541 cgtgaacggt gagcccgtcg gggagatcta ccttaacaac ctggccaatg gcggcatcga
      601 cctggcgccg ttccacaact tcgatagcgc catctcggac tccaccaaga ccgagctgga
      661 cgcgctgaaa gcggccatca tcgatggctc ggtgaaggtc agcgactact tcgctccggc
      721 gcagtagaca tcgactagca ccagttgcaa caggcggcgt cttcggacgc cgcctgtcgt
      781 ctatctgagg ggggattggg ctagagggtc gggagaggcg tgaagctcga acttcgcggc
      841 ataaccaagc gctttccggg ggtgctcgcc aacgacgaca taagcctgag ggtgggcgac
      901 ggcgagattc tgggcttgct gggcgaaaac ggtgccggca agacgaccct catgaacatt
      961 ctctacgggc tgtaccagcc ggacgccggc gcgatcctca tagacgacaa gccggttcgc
     1021 ttcagcggac cgggtgacgc gatcgccgcg ggcatcggca tggtccacca gcactttatg
     1081 ctcgtgccgg tgttcaccgt cgccgagaac gtcatgctcg gcgttgagtc gaccaagacc
     1141 gtcctcggca tgctcgatct cgccgcagtg cgccagcgca ttcgcgagat ctctgagcgc
     1201 cacaacctgc gcgtcgatcc ggacgcgcga gtcgaagatc tcgcagtggg cgtgcgccag
     1261 cgggtcgaga tcattaagac cctgtaccgc caatcgcatg tgctcgtgct cgatgagccg
     1321 accgccgtgt tgaccccgca ggagggcgag gagctgttca cgatcatgcg ctcgctcgtc
     1381 gccggtggga cctcaatcat cttcatcacc cacaagctgc atgaagtgct cgaggtggct
     1441 gaccgcatcg ccgtcctgcg ccgcggccga gtcgttggca cgacgacccc agccgagacc
     1501 agcgccgagc agttggcgac gatgatggtc gggcgcgaag tcgacctgat tgtggacaag
     1561 gcatcggcca agccgggcga gcccgtgctg gaggttcatg gcctgcacgt gcgcgacgat
     1621 cggggccacc tcgccgtcaa cgacgtcgac ctcacggttc acgcgggcga gatcgtggcc
     1681 gtcgcgggcg tccagggaaa cggccagacg gagctcgcgg aatcgatcac cggattgcgc
     1741 tcgcccgagc tgggcgagat aaagatcggc ggtcagcccg tgcgactcaa cccgcgatcc
     1801 gtttatcgcg agggagtggg tcacgtcccg gaggaccgca tggaggaagg gctcgtgccc
     1861 gccttcaacg tcgccgagaa cctcgttctg aacagctatt gggcaccgcc gtacggccat
     1921 ggcctggcgc tggatcggga caagatggcg agcgtcgccg aagaacgcat caaggaattc
     1981 gacatccgaa ccccgtctgt gttcaacccg gttaccaatc tgtccggcgg caaccaacag
     2041 aaggtcatcg tggcgcgcga attctcgcga cccctgaagc tgttggtcgc ggtccagccg
     2101 acgcgcggcc tggatgtcgg ttcgatcgaa tacatccacc ggcggctggt gcagaagcga
     2161 gacgaaggag ccgccgtgct tctggtttcg accgagttgg atgaggtcac ggcgctcggc
     2221 gatcgaatcg cggttatgta ccagggcaag atcgtcagca tgctcagtcc ggaggaggcc
     2281 acgttcgaga agctcgggct gctgatgggc ggtgccaacc ccgagggaca ttcgccagaa
     2341 gaggttctgg ccatggcggg caaggcccaa tgagcgacga actgatcgtg ccggaaccgg
     2401 cgcccgacgc ggacgccccg gccggcgccg tgggtcagcc ccgtcggaat gcgtggtcga
     2461 gggtcgccgg ctggctccag ccgctggtct tgccggcact cgcgctgttc agcgccgctg
     2521 ttgtaggagc cgtggtcatc atcgccggca accgcgatgt cgtcggcctg ctggagagag
     2581 accctgccgc tggcctgagc gctgcctggt cgatcgtggt cgagtcctac gcggcgctga
     2641 tccgcggctc gctgggcgac ccggcccaaa tcgcctcggc gctcgccggc ctcaatccga
     2701 ccgcattgat cgacgcgttc catccgttgt ccgaatcggt actgtcaatg acaccgcttg
     2761 tgctcacggg actatctgtg gcgctggcgt tccgagcggg cctcttcaat atcggcggcg
     2821 agggccagct tcagatgggc gcgctcgccg cggtgttcgt gggcttcagc ttccagggcc
     2881 tgccttggtt catccacctg ccgttggcgc tgcttgccgc cttggctggc ggcgccctgt
     2941 ggggcttgat accggcagtg ctgaaggcgc gcaccggcgc gcacgaggtc atcaccacga
     3001 tcatgctcaa tttcgtggcc gtgcaattgg tgttcttctc gctccgatcg ccactattcc
     3061 agcgtccggg ccggtccgat ccgatctcca aggtgatcga gccgtcggct gagcttcccc
     3121 ttcttgtcga gggtctacgg gcacattggg gcatcgtcct ggccttgatc gcggccatct
     3181 ttgtatcttg gttgctcttc cgttccacga agggctttga gttccgggcg gtggggctca
     3241 accccatcgc ggcgcgctac gccggaatgg ggatcggcgg gacgatcgtc gcctcgatga
     3301 tgatcgcggg cgccctgtcg gggttgggtg gcggcgtgat cgtcctgggt accgtccaca
     3361 acctgattcc cgcactcggc ggcggcgccg gtttcgacgg tattgccatc gccctgctgg
     3421 gtcgctccag accggccggc gtcgtggccg cggcattcct gatgggcgcg ttacgtgccg
     3481 gatctacacc catgcaggtg gccaccggca cgccgagcta tctcgtcacg gtgatccagg
     3541 cgctgatcat catgttcgtc gccgcacccg cgctcgtgcg agccatctat cgcatccgtg
     3601 ccgagcgtac gaccggcact gaaaccttcg cgaaagggtg gggcgcatga ccgccgccag
     3661 cgtcgccgct gccagcggcc gggttatgag cctgagcgaa cgcgctgcgc tgcggcgtca
     3721 acgcattctg ggcttcatct accttggcgc agccgcggtc atcgttttcg ccttcgcgtt
     3781 gccgattggc ccggacgtcc agtcgaagtt tgtgctggcg ctgggcccgg gcgacatcgc
     3841 cgttccaccc ttgatcctgc cttcgacgct cacgctgtac ttcctcgcgg gacttgccgc
     3901 gtttgccggt gcggcgcaac tcgtacgcgg cttcggatcc tggtggatcg tcgcgctggc
     3961 gctcgtcgcc ttcgcgttcg tgttcagctt cctgacctgg gtcgcggccg agggctcgat
     4021 gaacctgacg gggctgttgc agacgaccgt ggcgcgctcc gtgcccatca tctttggtgc
     4081 tctgtcgggc atcctgtgtg agcgctcagg ggtgatcaat atcgccatcg aggggatgtt
     4141 gctctcgtcg gcattcgccg ccgcggtgac ggccagcgtc acaggcgatt actggagcgg
     4201 gattctggcg gccatgatca ccggcgccct aatggcgcta ttgctcgggg tactggcaat
     4261 ccgctaccag gtcgaccaga tcgtcgcggg cttcgtgatc aacatcctcg cgaccgggct
     4321 gacgagctac cttcaccaag ccgtgctcgt ccccaacgcg acgctcaacc agccgccgcg
     4381 catcgaccgg ctgccgattc cgctcctatc agacattccg atcatcggcc cgatcttctt
     4441 ccagcacaat ctgttcgtct acacggcgtt catcctggtg ggcgtcatct ggttcgggtt
     4501 gttcaagacc aggtggggct tgcgcgtgcg ggcagtcggc gagcatccgc gggctgctga
     4561 caccgtgggc atcaacgtga acttcgtgcg ctaccgcaac gtcatcctgg gcggcatggt
     4621 ggcgggcatc ggcggcgcct atttcacgat cggctccacc ggcaccttcg aacgtgagat
     4681 gaccggcggt cgcggcttca tcggcctcgc ggcaatgatc ttcggcggtt ggaacccggt
     4741 gggcgccgca gggtcgggcc tggtatttgg atttgctgac gcgatccagg cacgcgccgc
     4801 gatcttcaag atcccggtgc catcggagtt cctgctgatg ctgccgtacc tcgtcaccat
     4861 catcgtggtt gccggcctgg tcggacgcgt ccgcgctcca gccgccgacg gcgtgccgta
     4921 caagaaggat tgaacgcgcc cgtcggtcac ggtcgaccgg tcatcctcga ctgcgatccg
     4981 ggacacgacg atgcgctggc aatgctgctt gcgctggcgc gtccggagat cgacctagcg
     5041 gcaatcacga cggtcgccgg caactcaacc ctcgataaca cgacgcgtaa cgcgctgtcg
     5101 gtcctgacgc tggttgggcg gacggacgtc ccggtcgctt ctggcgcggc cgcgccactg
     5161 cagcgtgagc tgatcacggc gcccaacgtc catggcggga gcggcctggc tggtgcagat
     5221 ctgcccgcgc ccgcggtcga tgttgccagc gacgatgcgg ccgcgttcat ggcccagctc
     5281 gtgcgccaga gccaagtccc gatcaccctc gtgccgaccg gaccgctgac caatgttgcc
     5341 cagttccagg cgcgcttccc tgacgagttt cggcgactgg agtcgatctg cctgatgggc
     5401 ggctcgatca cggagggcaa cgtgagcgct tccgcggagt tcaacatctg ggtcgatccg
     5461 gaagcggcgc gcgccgtgtt cgaaagcggc ctgccggtga cgatgatccc gctcgacgtc
     5521 acccaccagg cgctcgtcag cctcaccgac gcggacagga tggaagcgtt cggcaatcgg
     5581 accgggatcg tattcgcgga tctactgcgg ttctttgcgc tgttccacat cgagcgttat
     5641 ggctggcagg gcgcgcccat ccacgatgcc gtcgcggtcg ccaacgtcgc gctgcccgac
     5701 atggttgagc ggcgcgctta ccgggtcgac gtggagaccg tgagcgagct gacgcgcggc
     5761 cggacagtcg ttgacctgca tggggtcagc cgccgcgcgc ccaacgcgca ggtgggcatc
     5821 cgcatcgacc agccgcgctt cgtggaggtc ttgctggacg cggtcgcgca attcccctga
     5881 atcgccgctc gaataggttt cctccgcagc cggcctctgg ccgctgctgc gcgcaaagcg
     5941 actgcttccc ggcgtgccgc cgggccgcat tcgttgcttg cgggcgtgcc atactcgccc
     6001 taagacggct cccgtgcgtg cccgcaggag ctgccgcgtc tccgcgccgc tctgatcggc
     6061 gtgcacgcag caggggagtg gtgagtggac ctcgcgggcg acacgggcgg actcgcgcac
     6121 aacggcggcg acctacaggg cgacctcgcc cacacgacgg tcaaggtctc gccgcgcagc
     6181 gtcgaagagg cgcccttggt gctgatcatc gaggacgagg agactcagcg cacgctgctg
     6241 gcacgcgttc tcgagcgcga gggctatcgc gtcatcggcc tcgcagacgg tgagaccggg
     6301 ctgcgcgcca tcgtcgagca ttcgccgcag ctggtgctgc tcgatctcaa cctgccgcgc
     6361 atggacggct acgagatctg ccgccagctc cgcgccgacc cgatgaccgc aacattgccg
     6421 gtcatggtca tcactgccca caccgcgccc ggtgacatgg tggccgcgct cgatgcgggc
     6481 gcggacgact tcctggccaa gcccgtccag cagtacgagc tgctcgcgcg catacgaagc
     6541 gccatgcgcc tgcgccgtgc catcacaagc ctggagcgcg caactcagat cgtcgccgcg
     6601 ctggcgaacg cggtggaggc caaggatctc aacctcgtgc atcactgccg ctggctggcc
     6661 catcacgcgg cgcgagtcgg cgccaacgtg gggctgagag gcgaggagct cgaggcggtc
     6721 gcctacggcg cgctgctgca cgacgtgggc aagatcggcg tgtccgagca cctgctgcg
//
LOCUS       DATBRX010000232        10367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_105294, whole genome shotgun sequence.
ACCESSION   DATBRX010000232 DATBRX010000000
VERSION     DATBRX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105294"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VIF08_08760"
     CDS             <1..475
                     /locus_tag="VIF08_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEY8136114.1"
                     /translation="VGVDAARWFFASRAPSTGIDFDIELAKKESSENPVYYVQYAHAR
                     ISSILRKATDEGLKTADSLAGGLADDPVALGLAKDLLRLPDIVSDAAEERETQSITAY
                     ATELATTFHAFYRDRRVVDASDPLTSSYRLALVDATRVTLAAALGLLGISAPDSM"
     gene            complement(496..1722)
                     /locus_tag="VIF08_08765"
     CDS             complement(496..1722)
                     /locus_tag="VIF08_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136115.1"
                     /translation="MKQPWVSFALGLALVAGCVDVAPTPSPTPTGAPTSAPSAQPTPE
                     LGSQVDTVVATVPAVRELDPTRDVPYEFITREQFREELIALTEAEIPVDVRRAEERLY
                     KRLGLLAEDADLDALVQELYGAQVLAYYQPENGHFYVIGGDRPFGATDKLVVAHEYTH
                     ALQDQHFDLKDTVSDYTQGDAQLAELAVIEGDATLTSQLWTTDNLDFGDLLQLLIDGF
                     SQLDAASLDGVPLVLRRQLEFPYTEGFVFATTLHDEGGFAAVNDALTTPPASTEQILH
                     PEKYYAGELPAGVAAPDLLDQLGPEWHVAYEQTLGELGIQIIAAGGEVPEGALPGLPV
                     DWPHADAAAGWDGDRLVMYESSDGHWRIEWTSAWDSAADAMEFKSRIDELSGTFTGVT
                     VTGINFTRVDVSIADE"
     gene            1951..2655
                     /locus_tag="VIF08_08770"
     CDS             1951..2655
                     /locus_tag="VIF08_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8136116.1"
                     /translation="MEIVSYGETCLRLRGKEGIVVTDAFPRIVGPTGRGLTADIATYS
                     HPDGQSSLGLQTMSEAAAAAKAKSKVKRADLAVPTSLEPAFLLDSPGEFEVHQVLITG
                     VRTYRDEESGAQRGANMCFVFELDGVHVVHLGDVGHLLTEAQVGEIGTVDVACVAVGG
                     ALTAARAAELVAQLDAKMIVPMPLDGADKPDGELSKFLHEMSVQGSEPAPKITVTASS
                     LPAETTVVQLEPRARS"
     gene            complement(2652..3974)
                     /gene="hutI"
                     /locus_tag="VIF08_08775"
     CDS             complement(2652..3974)
                     /gene="hutI"
                     /locus_tag="VIF08_08775"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY8136117.1"
                     /translation="MTGDRPGLLVFNATEVATLAGGLRRGAAQSEVGLLMAEGSEPTG
                     APAVAAYQGRIVASGRLPEVEARLIELGVAEGAIHRIDAQRGTVTPGLIDPHTHLLFA
                     GTRHAEVELRQRGHSYLEILAAGGGILQTVRLTRAASDDELLDHGRRWLAEMLSHGVT
                     TAEVKSGYGLDTASELRLLALYGRLATDGPVELVPTFLGAHAVAPEFRDRPDAARAYI
                     DSVINEQLPAVAEQGIALSCDVFCEPSVFSVDDSRRLLTRARELGLQVRLHADQIATG
                     GGSELAAEVGALSADHLGAVSDAGIAALAKAADDGHPVVATLLPVSAFYLDEAHHAPA
                     RRLIEAGVPIALGTDFNPGTSPAPNAQLAMSFAIHGLRMTAPEALVALTINAAAALGI
                     ADSHGSLEPGKHADLVVWQPDSHALLPYWLGANLVRAVIKRGRVAYAS"
     gene            complement(3971..4942)
                     /gene="ftcD"
                     /locus_tag="VIF08_08780"
     CDS             complement(3971..4942)
                     /gene="ftcD"
                     /locus_tag="VIF08_08780"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEY8136118.1"
                     /translation="MAGLVECVPNFSEGRRPDVVDAIAGRVRATAAVHLLDRTSDADH
                     NRSVLTFAGPAEAVTGAMEGAVEEAIARIDMNAHTGQHPRIGAVDVVPFVPLAGTTMD
                     EAIELARGFGERIAQRFDLPVFLYAKAAQRSDREVLADIRRPQYEGLRDLIGEPGNAP
                     DFGPARMHPTAGAVAVGARPFLIAYNINLETRDLDLAKDIARRVRERSGGLPRVQALG
                     LDLAELGCVQVSMNLLDFSVTPMWRVWETVTAMAADEGVAARESELIGLVPLAALVDV
                     AAHADVAADLPVELRITRAAAWLKARDFEPTMALELRLAAAQADGAQ"
     gene            5063..5521
                     /locus_tag="VIF08_08785"
     CDS             5063..5521
                     /locus_tag="VIF08_08785"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEY8136119.1"
                     /translation="MEREVISNCPVCEARLQVTRLHCNTCGTTIEGEFNVGRFARLGR
                     DQMSLLESFLRSRGNLRELERELGLSYPTVRGRVETLLRTLGLGDGPAQPAETAAPAA
                     EPAIDPELRRSVLERLARHELTAEQAAAALRGEPLPEEAADAATGEKSND"
     gene            5514..6467
                     /locus_tag="VIF08_08790"
     CDS             5514..6467
                     /locus_tag="VIF08_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY8136120.1"
                     /translation="MTDNEQYRDPAEIRHHIGATGAFSLGNVSGDIRIRGTDGDEAVV
                     KARGRGRNEWLPINVRRGEGMLSIEIDHHKGPGLMGWRGRNDVEVEFDIVLPHGARVD
                     VNSVSADLDVTGMVGDQTYQTVSGDVAIVRTGGRINLTTVSGDAEVSADEPLEAEVNS
                     TSGDLELTGPVLRAAKLRTVSGDIEVRGSFEPGAMHGIETVSGDVQIESAGGLTVEVR
                     KGIDLGGGPKQRVVGDGAARLRFRSLSGDLHVMGVGGEKAVKDQPQSPPTPYAPPAPP
                     APPAPDNTMPPVSSLNSLEILRALERGEIDVEEAQRRLGGA"
     gene            6471..6830
                     /locus_tag="VIF08_08795"
     CDS             6471..6830
                     /locus_tag="VIF08_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136121.1"
                     /translation="MPDVTKILNLVAEGKLSAQEADEILAALNAREAGVSPNDTPPAA
                     PAHKTTHLRIEVTDGGRQVVNLRVPINIAGWASSFLPGLSDQDSDRIRGAVESGTRGT
                     ILDVTDDDGSRVLITSE"
     gene            6841..7050
                     /locus_tag="VIF08_08800"
     CDS             6841..7050
                     /locus_tag="VIF08_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136122.1"
                     /translation="MLLNDIGHAANDIQADAARTASWARQYETAVAARRVRGTRSVIG
                     HALINLGRFIAAEPAPARTQLRVGR"
     gene            7073..8398
                     /locus_tag="VIF08_08805"
     CDS             7073..8398
                     /locus_tag="VIF08_08805"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8136123.1"
                     /translation="MTTVTASKSPLRVRDFRLFWLGEAISTLGDQFAMIALPWLALIL
                     TGDAFALGMVLALMALPRALFMLVGGAYVDRLSPRLVMLGSNAVRLVAVGVLGFVVLE
                     GAAAMWMLYAFALAFGVADAFFYPALTAMVPDLVDDSQLQRANGIVQGTAQFTTLIGP
                     AVAGVAIAALASQSAGPDVTGVALALLFDAATFVASLATLFLITARRAHAEASGSVIK
                     DIREGIGFVWKMPAVRVMVLLSMAANMLIVGPIDVGLPIVAYSRLPEGAAAFGLIMSA
                     FGGGSLLGMVGATVLPPLPRAHFGSILLALFSLSGVCLALLALFSSTPIVLLDAVIAG
                     TILGYSNITYITWLQRRIPRQLMGRVMSLMMFGSVALVPISMALSGALVKISLDAVLV
                     GGGLGMAGLAVVGLLSRSVRAMGLEPTVNEDESAEETAPTTSAEAGATA"
     gene            8438..9706
                     /locus_tag="VIF08_08810"
     CDS             8438..9706
                     /locus_tag="VIF08_08810"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8136124.1"
                     /translation="MRRPTVPQVLRDANFARFLTATTISTLGGGMATIALAFAVLDHG
                     GVTELGIVLLAREVPMIVFVLLGGVFADRLRRRTILVSTDLVKAATQGLTAFLFFTGT
                     ADVLTVSLLQIVFGVANAFSRPAYTGLVREILRDDQLQEANALLGLSNSVLHIAGPAL
                     GALIVAAGSPALALAIDAVTFLISALLMFTMRIARVIRIASTSVLADLRSGWRELISR
                     PWLVAMILSFGMFQLSFFPALGVLGPAVARDSLGGPTAWGLILSLESVGAVIGGLVAL
                     RIRVSRPLIASQLFCLPCGLLLIGLGVPLNVALLALLSAGVGFGFAAGNTFWMTALQQ
                     NVPEHALSRVSSFDWLGSVAFNPLGYLLIGPLATAIGTSQALIASGVINLATAVGVLL
                     VPSVRAIRMVGAPAAADSDGEPPGPLTELA"
     gene            9742..>10367
                     /locus_tag="VIF08_08815"
     CDS             9742..>10367
                     /locus_tag="VIF08_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136125.1"
                     /translation="MATIDDLARGIARDLSPEFEKRIRDALAQQDREWLIDELVRLTL
                     SRHRLQDLERDAAATALLRAERLQRVVRLKLDESAVKRFIGDNADRDRARLIADGFLN
                     ADAPEKGTQMLRGEHRSKQGDALLVGAKDMLFGLLFGDASTGTKLDRVQQELLTLALP
                     RAKADALDFMRASTELAAAGTWQDPDSVSNDERADNVLLEVQFGETAD"
BASE COUNT         1579 a         3578 c         3491 g         1719 t
ORIGIN      
        1 ggtgggcgtt gacgccgcgc gctggttctt cgcctcgcgc gcgcccagca ccggcatcga
       61 cttcgacatc gagctcgcca agaaggagtc gagcgagaac ccggtctact acgtgcagta
      121 cgcgcacgcg cgcatcagct cgatcctgcg caaggccaca gacgagggcc tgaagaccgc
      181 cgattcactc gctggtggcc tggccgacga cccagtagcg ctcgggctgg ccaaggatct
      241 gttgcgcctg cccgatatcg tcagcgacgc ggccgaggag cgcgaaacgc agtcgatcac
      301 cgcgtacgcg accgagctcg ctacgacctt ccacgcgttc taccgcgacc ggcgcgttgt
      361 cgacgcgtcc gacccgctga cgtccagcta tcgcctcgcg ctggtcgatg cgacgcgcgt
      421 cacattggcc gccgcactcg ggctattggg gatctccgcg cccgactcga tgtagggctc
      481 cgcaggaggc cgcggctatt cgtcggcgat gcttacgtcg acgcgcgtga agttgatgcc
      541 ggtcaccgtc acgccggtga acgtgccgga gagctcatcg atgcgcgact tgaactccat
      601 tgcgtccgcg gcgctgtccc aggcgctggt ccactcgatg cgccagtgcc cgtcggagct
      661 ctcgtacatc accaggcgat cgccatccca accggccgcc gcgtcggcat gcggccagtc
      721 aacgggtaga ccgggcaacg cgccctccgg cacctcgccg cccgcggcaa tgatctggat
      781 cccgagctca cccagagtct gctcgtaggc gacgtgccac tcgggaccca gctgatcgag
      841 cagatcaggc gcggcgacgc cggccggcag ctcgcccgcg tagtacttct ccggatgaag
      901 gatctgctcg gtcgaggcgg gcggcgtcgt gagtgcgtcg ttcaccgccg cgaagccgcc
      961 ctcgtcgtgc agcgtcgtcg cgaagacgaa gccttccgtg tacgggaact cgagctggcg
     1021 gcgcaagacc agcgggacgc cgtcgagcga cgccgcgtct agctggctga agccatcgat
     1081 gagcagctgc aggaggtcgc cgaagtcgag gttgtcggtc gtccagagct gcgaggtcag
     1141 cgtcgcgtcg ccttcgatga ccgccagctc ggcaagctgg gcgtcaccct gcgtgtaatc
     1201 gctgacggtg tctttcaggt cgaagtgctg gtcctgcagg gcgtgggtgt attcatgagc
     1261 cacgacgagc ttgtcggtcg cgccgaacgg ccggtcgccg ccgatcacat agaagtgccc
     1321 gttctccggc tggtagtagg ccagtacctg cgcgccgtac agctcctgga cgagcgcgtc
     1381 cagatcggcg tcctccgcca gcaggccaag gcgcttgtag agccgctcct ccgcccggcg
     1441 tacgtccacc ggtatctccg cctccgtgag cgcaatcagc tcctcccgga actgctcgcg
     1501 ggtgatgaac tcatatggca cgtcgcgtgt cgggtcgagc tcacgcacgg caggcacggt
     1561 cgcgacgacc gtgtcaacct ggctgccgag ctccggggtg ggctgcgctg atggggctga
     1621 cgttggcgcg ccggttggcg tcgggctggg cgtcggcgcg acgtcgacgc agccggcgac
     1681 cagtgccaga cccagcgcga atgagaccca gggctgcttc acggagccga ttctgacagc
     1741 gcgggccgcc atcgcatttc cacattcggt gtcggtttct tcaccgtctc gggtctggtg
     1801 gctcaacccg gcctgaatag tctgcgaacc agcggaaccg tgaagcaccg cttcccgttc
     1861 cctctccctc cctcggaacg aggggcgcca attgggcagg cgcccctcgt ttctttttgc
     1921 gttcgctctc tttgcccgac aatggggcag atggagatcg tttcgtacgg tgagacctgc
     1981 ctccggctgc gcggcaagga gggcatcgtt gtcactgacg ccttcccgcg catcgtcggc
     2041 ccgaccggcc ggggcctgac agccgacatc gcgacctaca gccaccctga cgggcagtcg
     2101 agccttgggc tccagacgat gtcggaggcg gctgccgcag cgaaggcaaa gagcaaggtc
     2161 aagcgcgccg atctggctgt cccgaccagc ctcgagccgg cgttcctgct cgattcgccg
     2221 ggcgagttcg aggttcacca ggtcctgatc accggtgtgc gcacctatcg cgacgaggaa
     2281 tcaggtgccc agcgtggcgc caacatgtgc ttcgttttcg agctcgatgg cgtacacgtc
     2341 gtgcatctgg gagacgtggg ccatttgctg accgaagccc aggtgggcga gatcggcacg
     2401 gtcgatgtcg cctgcgtcgc cgttggcggc gcgctgacgg cggcgcgtgc cgcggagctc
     2461 gtcgcgcagc tggacgccaa gatgatcgtg cccatgccgc tcgatggcgc cgacaagccg
     2521 gacggcgagc tgtcgaagtt cctgcacgag atgagcgtcc agggcagcga gccggcgccc
     2581 aagatcaccg tgacggcgtc gtcgctcccc gccgaaacga ccgtggtcca gctcgagccg
     2641 cgcgcccgct cctagctggc gtaggccacg cggccgcgct tgatgaccgc acgtacgagg
     2701 ttcgcgccca gccagtaggg cagcagcgcg tgcgaatcgg gttgccagac gacgagatcg
     2761 gcgtgcttgc ccggctcgag tgagccgtgg ctgtcggcga tgcccagcgc ggcggccgca
     2821 ttgatggtca gcgccacgag agcctccggt gcggtcatgc gcaggccgtg aatcgcgaaa
     2881 gacatcgcca gctgcgcgtt cggcgcgggt gaagtgcccg gattgaagtc cgttcccagg
     2941 gcgatcggga cgccggcctc gatcaggcgc cgcgcgggcg cgtgatgcgc ctcgtcgaga
     3001 tagaaggcac tcaccggaag aagcgtcgcg acgaccgggt ggccatcgtc cgcggccttc
     3061 gcgagggcag cgatcccggc atccgacacg gcgcccagat ggtcagccga gagcgcaccc
     3121 acctccgccg ccagctccga cccaccgccg gtggcgatct ggtcggcatg cagccgaacc
     3181 tgcagaccca gttcgcgggc gcgcgtcagc agccggcgtg agtcgtcgac cgaaaaaacg
     3241 ctcggttcgc agaacacgtc gcacgagagc gcaatgccct gctccgcgac agcgggcagc
     3301 tgctcgttga tgacgctgtc gatgtaagca cgcgctgcgt caggccggtc gcgaaactcc
     3361 ggcgcgacgg cgtgggcgcc aagaaaggtg ggcacgagct cgaccggccc gtctgtggcg
     3421 agccggccgt agagcgccag caggcgcagc tccgacgccg tatccagccc atagccgctc
     3481 ttcacctcgg ctgtcgtcac gccgtggctg agcatctcgg ccagccagcg ccggccgtgg
     3541 tcgagcagct cgtcgtcgga cgcggcacgc gtcaagcgca ctgtctggag gatgccgccg
     3601 cccgcggcca ggatctccag gtaagagtgg ccgcgctggc gcagctccac ctccgcgtgc
     3661 cgcgtgccgg cgaacagcag gtgcgtgtgc gggtcgatca gcccgggcgt gaccgttccc
     3721 cgttgcgcgt cgatgcggtg gatggcgccc tcggccacgc caagctcgat gaggcgcgcc
     3781 tcgacctcgg gcagccgccc gctggcgacg atacgtccct ggtacgccgc cacggccggc
     3841 gccccagtgg gctcactccc ctccgccatg agcaagccga cctccgactg ggcggcgccg
     3901 cggcgcagcc cgcctgcaag cgtggccacc tccgtggcgt tgaagacgag caggccgggt
     3961 cggtctccgg tcactgtgca ccgtcggctt gagcggccgc gagccgtaat tcgagcgcca
     4021 tggtcggctc gaaatcgcgc gccttcagcc acgccgccgc ccgcgtgatc cgcaactcga
     4081 ctggcaggtc ggccgccacg tcagcgtggg ccgccacatc taccagcgcc gcgagtggga
     4141 cgagcccgat cagctccgac tcgcgcgccg ccaccccttc gtcggccgcc atcgccgtca
     4201 cggtttccca gacacgccac atgggtgtga ccgagaagtc gagcaggttc atggacacct
     4261 ggacgcagcc cagctcggcc aggtccaggc ccagcgcctg gacacgcggc agtccgccgg
     4321 atcgctcgcg gacgcggcgg gcgatgtcct tagccaggtc caggtcgcgt gtctcgaggt
     4381 tgatgttgta ggcaatcagg aacggccgcg ccccaaccgc gacagcgcct gcggtggggt
     4441 gcatccgcgc cggtccgaag tcgggcgcgt taccgggctc gccgatcaaa tcgcgcaggc
     4501 cctcgtactg cggccggcgg atgtcggcca gcacctcgcg gtcggagcgc tgcgcagcct
     4561 tggcgtacag gaagaccggc aggtcaaagc gctgggcaat gcgctcaccg aagccgcgcg
     4621 ccaattcgat tgcctcgtcc atggtcgtgc cggcgagcgg cacgaagggg acgacgtcga
     4681 ccgcgccaat gcgcgggtgc tggccggtgt gagcgttcat atcgatgcgc gcgatggctt
     4741 cttccacggc gccttccatc gccccggtga ctgcctccgc cggcccggca aaggtcagca
     4801 cgctccgatt gtggtccgca tcggacgtcc gatcgagcag atggaccgct gcggtggccc
     4861 tgacccgacc ggcaatggca tcgacgacgt cgggccggcg gccctcgctg aagttgggga
     4921 cgcattcgac gaggccggcc atggcgccaa gtctagtcag ccatgcacaa gttgccctta
     4981 tcggcaccgt gtggcaagcc agtctttcac tttgttgagt tcctacttga caatgcgtaa
     5041 ctccgtttct atattgcgcg ccatggaacg agaagtcatc tccaactgtc ccgtctgtga
     5101 ggcgcgcctc caggtcacca ggctgcactg caacacgtgt ggcacaacca tcgagggcga
     5161 gttcaacgtg ggccgtttcg cccgcctggg gcgcgaccag atgtcgttgc tcgagtcctt
     5221 cctccggtcg cgcggcaacc tgcgcgagct ggagcgcgag ctcggcctct cgtatcccac
     5281 cgttcgcggc cgcgtcgaga cgctgctgcg caccctgggc ctgggcgacg gtcctgccca
     5341 gcctgcggag accgccgctc cggcggcaga gcccgccatt gatccagagc tgcgccgctc
     5401 cgtcttggag cgactggcgc gccatgagct gactgcagag caagctgccg ccgcactgcg
     5461 cggcgagccg ctgccagaag aggcggccga tgccgcaacc ggggagaagt ccaatgactg
     5521 acaacgagca atatcgggac ccggcggaga ttcgccacca catcggcgcc acgggcgcgt
     5581 tcagcctggg caacgtctcg ggcgacatcc ggatccgcgg caccgacggc gacgaggccg
     5641 tcgtcaaggc gcgcggccgg ggacgcaacg agtggctgcc catcaacgtg cgccgcggcg
     5701 aggggatgct gagcatcgag atcgaccacc acaagggccc gggcctcatg ggctggcgtg
     5761 gtcgcaacga cgtggaggtg gagttcgaca tcgtcctgcc gcacggcgcg cgcgtcgacg
     5821 tgaactccgt cagcgccgat ctcgacgtga ctggcatggt cggcgaccag acctatcaaa
     5881 ccgtctcggg cgacgtcgcg atcgtccgca ccggcggccg gatcaacctc acgacagtgt
     5941 cgggcgatgc cgaggtcagt gccgacgagc cgttggaagc cgaggtcaac tcgacatccg
     6001 gcgatctcga gctgaccggc cccgttctgc gcgcggccaa gctgcgcacg gtcagcggcg
     6061 acatcgaggt tcgcggctcg ttcgagccgg gcgcgatgca cggcatcgag accgtcagcg
     6121 gcgatgtcca gatcgagtcg gccggtggtc tgacggttga ggtccgcaag ggcatcgacc
     6181 tcggcggcgg gcctaagcag cgtgtcgttg gcgatggcgc cgctcggctg cgcttccgct
     6241 cactgtccgg tgacctgcat gtcatgggcg tgggcgggga gaaggccgtc aaggaccagc
     6301 cgcagtcacc gcccacgccg tatgcgcccc cggctccgcc ggccccgccc gcgccggaca
     6361 acaccatgcc tcccgtctcc agcctcaact cactcgagat cctgcgagcc ctggagcgcg
     6421 gcgagatcga tgtcgaagag gcgcagcgcc gcctcggagg agcttgaccc atgcctgacg
     6481 tcacgaagat tctgaacctg gttgcagagg gcaagctcag cgcccaggag gcggacgaga
     6541 tcctcgccgc gctgaatgca cgggaggccg gcgtttcgcc gaacgacacg ccccccgccg
     6601 ctccggcgca caaaacgaca cacctccgaa tcgaggtcac cgacggtggc cgccaggtag
     6661 tcaacctgcg tgtgcccatc aacatcgccg gctgggcgag ctcgttcctg cctggcctct
     6721 cagatcaaga ctcggaccgc attcgcgggg cggtcgaatc gggtacgcgg ggaaccatcc
     6781 tcgacgtcac cgacgacgac ggcagccgcg tcctcatcac cagcgaatag gagacaggaa
     6841 atgctgctca acgacatagg ccacgcggct aacgacatcc aggccgacgc cgcccgcacg
     6901 gcgtcatggg cccgccagta cgagaccgca gtcgcggccc gcagggtccg cggaacccgc
     6961 tcggtcatcg gccacgcgct tatcaacctc ggtcgcttca tcgcggccga gcccgccccc
     7021 gcccgaacgc agctgcgcgt cggccgctag caacgacgag gacgaagccg ccatgaccac
     7081 agtcaccgcc tcgaagtcgc cgctccgggt gcgcgacttc cggctgttct ggctgggcga
     7141 ggccatttcg acgctgggcg accaattcgc gatgatcgcg ctgccgtggc tggcgctgat
     7201 cctgaccggc gacgcgttcg cgctgggcat ggtgctcgca ctgatggccc tgccgcgcgc
     7261 gctcttcatg ctcgtcggcg gtgcctacgt cgatcggctc tcgccgcgcc tggtgatgct
     7321 cggctcgaac gccgtgcgcc tcgtggcggt gggcgtcctg ggcttcgtgg tcctcgaggg
     7381 cgccgcggcc atgtggatgc tctacgcctt cgcgctcgcg tttggtgtcg ccgatgcgtt
     7441 cttctacccg gcgctgaccg cgatggtgcc cgacctggtg gacgactcgc agctccagag
     7501 ggcgaacggc atagtccagg gcactgcgca attcacgacc ctgatcggac cggcggtggc
     7561 gggcgtcgcg atcgcggcgc tggccagcca gtccgccgga cccgatgtca cgggcgtcgc
     7621 cctggccctc ctcttcgacg cggcgacgtt tgtggcgtcg ctcgccacgc tcttcctgat
     7681 cacggcccgt cgcgcccacg cggaggcgag tggctcggtc atcaaggaca tccgcgaagg
     7741 catcggcttt gtttggaaga tgcctgcggt gcgggtgatg gtgctgctga gcatggccgc
     7801 caacatgctc atcgtcggcc cgatcgacgt ggggctgccg atcgtcgctt acagccggct
     7861 gccggaaggc gcggccgcat tcggcctgat catgtcggcg ttcggtggcg gctcgctcct
     7921 tggcatggtt ggcgcgaccg tgttgccccc gctgccgagg gcgcatttcg gctcgatcct
     7981 gctcgccctc ttctcgctca gcggcgtctg cctggcactg ctcgcgctgt tcagctcgac
     8041 gcccattgtg ttgctcgatg cagtgatcgc gggcacgatc ctgggctact ccaacatcac
     8101 ttacatcaca tggctgcagc ggcggattcc gcgccagctg atgggccgcg tcatgagcct
     8161 gatgatgttc ggctcggtcg cgctggtacc catctcgatg gcgctctcgg gcgcgctggt
     8221 caagatcagc ctcgacgcgg tcctggtggg cggcgggctg ggcatggctg ggctggccgt
     8281 cgttggactg ctctcgcgca gcgttcgagc gatgggcctg gagcccacag tgaacgagga
     8341 cgaatcggcc gaggagacgg ccccgacgac cagcgcggag gcgggcgcaa ccgcttaagc
     8401 cgcgggcgcc tcgcggccga actagactgc gcgcccgatg cgccgaccca ccgtccccca
     8461 ggtcctgcgt gatgccaatt tcgcgcgctt tctcaccgcg acgacgatct cgacgctggg
     8521 cggcggcatg gccaccatcg ccctggcttt cgcggtcctc gaccatggcg gagtaaccga
     8581 gctcggcatc gtcctcctcg cgcgcgaggt cccgatgatc gtcttcgtcc tgctgggcgg
     8641 tgtcttcgcc gaccggctgc ggcgccggac gatcctggtg agcactgacc tcgtcaaggc
     8701 cgctacccag ggcctgacgg ccttcctctt cttcaccggc acggcggatg tcctgacggt
     8761 cagcctcctg cagatcgtgt ttggcgtcgc caacgccttc tcgcggcctg cgtataccgg
     8821 tttggtgcgc gagatcctcc gggacgatca gctccaggaa gccaacgcac tcctggggct
     8881 gtcgaacagc gtcctgcaca tcgccgggcc tgcgctgggc gcactcatcg tcgccgccgg
     8941 cagcccggcg ttggcgctgg caatcgacgc cgtgaccttc ctgatttccg cgctcctcat
     9001 gttcacgatg cgcatcgcgc gcgtgatccg cattgcctcc acgtcggtgc tggccgacct
     9061 gcgcagtggc tggcgcgagc tcatcagcag accgtggctc gtggcgatga tcctctcgtt
     9121 cgggatgttc cagctgagct tctttccggc gctgggcgtc ctcgggccag cggtcgcccg
     9181 ggacagcctt ggcggtccca cggcgtgggg attgatcctg tcgctggaat cggtcggagc
     9241 ggtgatcggt ggcctggtgg ctttgaggat cagggtcagc cgcccgctca ttgccagcca
     9301 gctcttctgt cttccgtgcg gactactgct gatcggactc ggcgtgccac ttaacgttgc
     9361 cctgctcgcg ctgttgagcg ccggcgtcgg gttcggcttc gccgcaggga acaccttctg
     9421 gatgacggcg ctgcaacaga acgtgcctga gcacgccttg tcgcgcgtca gctcattcga
     9481 ctggctgggt tcggtggcat tcaacccgct cggctacctc ttgatcggac ccctggccac
     9541 ggccatcggc acatcgcagg cgctcatcgc gtcgggcgtc atcaatctcg ccactgccgt
     9601 tggcgtgttg ctcgttccgt ccgtgcgcgc gatccgcatg gttggcgcac ctgcggcggc
     9661 cgactctgat ggcgagccac ccggtccgct gaccgagctc gcctgagaaa cgtcgtcgtc
     9721 tgaggctaat ctctgccgga gatggcgacg atcgacgacc tcgcgcgcgg catagcgcgc
     9781 gacctttcgc ccgagttcga gaagcggatc cgcgacgcgc tggcgcagca ggaccgcgag
     9841 tggctgatcg atgagctggt gcgcctgacc ttgtcacggc accggctcca ggatctcgaa
     9901 cgcgacgcgg ccgcaaccgc tttgctgcgc gccgagcggc tccagcgggt ggtcaggctc
     9961 aagctggacg aatcggctgt caaacggttc atcggcgaca acgcggatcg cgaccgggcg
    10021 aggctgatcg ccgacggctt cctgaatgcc gacgcgccgg agaagggcac gcagatgctt
    10081 cgcggtgagc accgctcgaa gcagggcgat gcactcctgg tcggcgcgaa ggacatgctg
    10141 ttcgggctgc tgtttggcga tgcttccacg ggcacgaagc tggaccgcgt ccagcaggag
    10201 cttctgactc ttgccctgcc aagagcgaaa gctgacgcgc tcgatttcat gcgcgcgtcg
    10261 accgagctcg ccgctgccgg cacgtggcag gatcccgact ccgtcagcaa cgacgagcgc
    10321 gccgataacg tgctgctgga ggtccagttc ggcgagacgg ccgacga
//
LOCUS       DATBRX010000233         6432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3010726, whole genome shotgun sequence.
ACCESSION   DATBRX010000233 DATBRX010000000
VERSION     DATBRX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010726"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /gene="dnaK"
                     /locus_tag="VIF08_08820"
     CDS             <1..1363
                     /gene="dnaK"
                     /locus_tag="VIF08_08820"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8136126.1"
                     /translation="VYDLGGGTFDISILELGDGVFEVKATNGDTHLGGDDFDQRVIDW
                     LLTEFKKDQGIDLSKDQQSLQRLKEAAEKAKIELSSTTTTEINLPYITVDADKNPKHL
                     VMSLSRAKLEDLVADLVERTRGPVEAALKDAGLKPTDIDEVILVGGQTRMPAVVEAVK
                     KMFGGKEPHKGVNPDEVVAIGAAIQAGVLAGDVKDVLLLDVTPLSLGIETLGGVMTRL
                     IDRNTTIPTSKSQTFSTASDNQPQVEIHVLQGEREMASDNKTLGRFILDGIPPAPRGV
                     PQIEVTFDIDANGILDVKAKDRATSREQQVRITASSMLSKNDVDKMVKDAQEHAEEDR
                     KRREVVDTRNDADQLAFQAERTLRDLGDKVQPADKADVETKVTALREALKGDDVEQIR
                     SRMNELAASLQKVSTAAYQAAGPSSSDGNGTGGAAPEGEPAGAAAGGPTEEEAVEGEF
                     KEV"
     gene            complement(1424..1912)
                     /locus_tag="VIF08_08825"
     CDS             complement(1424..1912)
                     /locus_tag="VIF08_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY8136127.1"
                     /translation="MRVSAKSDYAVRAAAELAAAGVGGHRKREQIADAQGIPSKFLET
                     ILLDLKHSGIVKSTRGADGGYALARPAADISIADIIRAVDGPMATVRGERVEQVSYEG
                     AARSLRDVWVAVRASLRRVLETTSLQDLVDDHLPDHVRELTADPEAWISLNRRRRPPH
                     DE"
     gene            2034..2996
                     /locus_tag="VIF08_08830"
     CDS             2034..2996
                     /locus_tag="VIF08_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8136128.1"
                     /translation="MPVPESAMTSDAARQPLISARALTKKFGTFVAVDAIDFDVAPGE
                     AFGFLGPNGAGKTSTMRMIGAVSPRSGGQLSVLGTDPDQHGARIRARLGVVPQEDTLD
                     TELTVLENLLIYGRYFDLSYAEARIRALELLDFVQLSDRAKTLVEPLSGGMKRRLTIA
                     RSLINEPDLLLLDEPTTGLDPQARHLVWDRLYRLKQRGTTLVLTTHYMDEAEQLCDRL
                     VVMDKAKIVAEGSPRALIEQYSTKEVLELRFPAGVQDTLDGQLDGLGDRVEKLPDRVL
                     VYAADGEAAATAAHERGLVPETILVRRSSLEDVFLRLTGRSLIE"
     gene            3010..3819
                     /locus_tag="VIF08_08835"
     CDS             3010..3819
                     /locus_tag="VIF08_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8136129.1"
                     /translation="MTAIAAPAPRGRAFNFPGLRKLEHSLFVYRRVWRGTFFGTLVSP
                     VLFLSAMGVALGSLVPSMTAFGGVPYLVFLAPGLLSAQAMQTGTIEGTWPLFAGFKWL
                     KTFDAMISTPITVRDIVLGHLYWLFVRIGIVTGIFMVVAFLFGAFTSPTFILAWPSAI
                     LTGVAFGICMSAWTATRKTEQSFSIIFRFIVTPLFLFSGTFFPIEQLPDQIEWVAWFT
                     PLFHGVALTRALAIGHDLNLVTLAIHLAVVLGLLVIGTLFAFRTFRKRLVI"
     gene            3820..4629
                     /locus_tag="VIF08_08840"
     CDS             3820..4629
                     /locus_tag="VIF08_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8136130.1"
                     /translation="MATTMTGSAVTRVLPFGSRHSVHILERNRFIFRRGWMVIFSGFF
                     EPLFYLLGIGFGLGSLIGTVAGPDGTPIPYAVFVAPALLATSAMNGAVYDSTNMFFRL
                     RYEKAYDGMLATPLNVGDITLGEVIWALGRGALYGLGFLAVMFAMGLVPAATGIFALP
                     ASMLIGFATAAAGLVAVTFMRSWQDLDLLFVITLPLFLFSGTFFPVSTYPEPLRTLVE
                     FSPLYRGVDLVRAMTTGVIGVAQLIDMVYLFAMGLLFVYIASRRMGKMLLK"
     gene            complement(4839..5882)
                     /locus_tag="VIF08_08845"
     CDS             complement(4839..5882)
                     /locus_tag="VIF08_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136131.1"
                     /translation="MPGFLEAVIASGNSVSGWNGLSIFGGVGQHGSLPTPFDAPAGNR
                     LWGPQGSHPLAAIGLHELVARSSNGIDGEITIKDPVKIGEALAGHIKLTARKDIKARG
                     AMFHLIGALLVEREQSREERDSKGNVTKSERWTEINGSVFEDLQLAETPLPATLDAGQ
                     TFEADFMLPAPRLGPVSGHLGSAAIVWALDAKWDISMGGDERVSALVNVKQNLDYLRS
                     GAVRLDDGAMFDVYGSGGATISVKPLPPLEAGAEFEVTVNWPGAGAGRGGRLELQADV
                     DAPNKLSGVVLFSQVVDPNAFKSGYTTKITIPADAPGIVNDKGVAVHYRIRALVDRAF
                     RSDLAVERFIAVI"
     gene            complement(5883..6272)
                     /locus_tag="VIF08_08850"
     CDS             complement(5883..6272)
                     /locus_tag="VIF08_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8136132.1"
                     /translation="MLNDAMIVATIPVTDLDVSLPFYRDALGLAVLDEGPFACRLGAG
                     STQLSIFKRPPVERGHTMAHFEVRDMDAAVADLKSRGVVFEEYESGPIATTNSIANYA
                     PGMRGAWFKDPDGNVLGLREGPQFGDW"
BASE COUNT         1027 a         2205 c         2095 g         1105 t
ORIGIN      
        1 cgtctacgac ctgggcggcg gcacgttcga catctcgatc ctcgagctgg gcgacggtgt
       61 cttcgaggtc aaggcgacca acggtgacac ccacctgggc ggcgacgact tcgaccagcg
      121 cgtgatcgac tggctgctga ctgagttcaa gaaggaccag ggcatcgacc tgtccaagga
      181 ccagcagtcg ctgcagcggc tcaaggaagc ggccgagaaa gccaagatcg agctgtcttc
      241 gacaaccacg acagagatca acctgccgta catcacggtc gacgcggaca agaaccccaa
      301 gcatctcgtg atgtcactgt cgcgggcgaa gctggaggat ctcgtcgcgg acctggtcga
      361 gcgtacgcgt ggcccggtcg aggccgcgct caaggacgcc ggactgaagc cgaccgatat
      421 cgacgaggtc atcctcgtcg gtggtcagac acgcatgccg gccgtcgtcg aggcggtcaa
      481 gaagatgttc ggcggcaagg agccgcacaa gggcgtcaac ccggacgagg ttgtggccat
      541 tggcgccgcg atccaggccg gcgttctggc gggcgacgtg aaggacgtgc tcctgctgga
      601 cgtcacgccg ctgtccttgg gcatcgagac cctgggtggc gtgatgacgc gtctcatcga
      661 tcgcaacacc accatcccga cgtccaagtc gcagaccttc tctaccgcca gcgacaatca
      721 gccgcaggtc gagatccacg tcctccaggg cgagcgcgag atggccagtg acaacaagac
      781 cctcgggcgc ttcatcctgg acggcatccc gccggcgcca cggggcgtgc cgcagatcga
      841 ggtcaccttc gacatcgacg ccaacggcat cctcgatgtg aaggccaagg accgcgccac
      901 ctcgcgcgag cagcaggtgc gcatcacggc gtcatcgatg ctgtcgaaga acgacgtcga
      961 caagatggtc aaggacgcgc aggagcacgc cgaggaagac cgcaagcgcc gcgaggtggt
     1021 cgacacgcgc aacgacgccg accagctcgc gttccaggcc gagcgcacac tgcgcgacct
     1081 gggcgacaag gtccaacctg cggacaaggc cgacgtcgag accaaggtca cggcgctgcg
     1141 cgaggccctc aagggcgacg acgtggaaca gatccgctcg cgcatgaacg agctcgccgc
     1201 gtcactgcag aaggtctcca cggctgccta ccaggccgct ggtccctcga gcagcgacgg
     1261 caacggcacc ggtggtgccg ctcctgaagg ggagccggcc ggcgctgccg caggcggccc
     1321 gaccgaggaa gaggccgtcg agggcgagtt caaggaggtc tagcctccgc gccaccaatc
     1381 gcacgaacgg aacgccgtcg gcactgcgcc ggcggcgttt ctgctactcg tcgtgcgggg
     1441 ggcggcggcg gcggttgagc gagatccagg cctcaggatc ggccgtcagc tcgcgcacgt
     1501 ggtcgggcag gtggtcatcg accaggtcct gcaggctggt cgtctcgagg acgcggcgta
     1561 gcgaggcgcg cacagcgacc cagacgtcgc gcagcgaacg cgccgcgcct tcgtaactga
     1621 cctgctcgac gcgctcgccg cgcacggtgg ccattgggcc gtcgaccgcg cgaattatgt
     1681 ccgcgatgct gatgtcggcc gccggccgcg cgagggcgta gccgccatcg gcgccgcgcg
     1741 tgctcttgac gatgcccgag tgcttgaggt cgaggaggat cgtctcgagg aacttggacg
     1801 ggatgccctg ggcgtcggca atctgctcgc gcttgcggtg gccgccgacg ccggctgcgg
     1861 ccagctccgc ggcggcgcga acggcgtaat cggatttggc tgaaaccctc attcctagtt
     1921 aattgtgggg gattgtgggc aacgtggcga gtggccatca ccaatgcccg cgccagcccc
     1981 cgcgcaaccc gccacgcagc ccgccgcgca gcccgccgcg ctaccatgcc gcggtgcccg
     2041 ttcctgagtc cgcgatgacg tccgacgccg cccgccagcc gctcatcagt gcgcgcgcgc
     2101 tgaccaagaa gttcggcact ttcgttgccg tcgacgcgat cgatttcgat gtcgcgccgg
     2161 gcgaggcttt tggcttcctg ggccccaacg gcgcaggcaa gacatcgacg atgcgcatga
     2221 tcggcgcggt gtcgccgcgc tctggcggtc agctgtccgt tctgggcact gatcccgacc
     2281 agcacggcgc ccgcatccgc gcccgactcg gcgtggtgcc ccaggaggac accctcgaca
     2341 ccgagctgac ggtgctcgag aacctgctca tctacgggcg ctacttcgac ctgtcctacg
     2401 cggaggcgcg tattcgtgcc ctggagctgc tcgatttcgt ccagctgagc gaccgcgcca
     2461 agaccctggt cgagccgctc agcggtggca tgaagcgccg cctgacgatt gcccgctcgc
     2521 tcatcaacga gccggacctg ctgctgctcg acgagccgac caccggtctc gacccgcagg
     2581 cacggcacct cgtttgggat cgcctgtacc ggctcaagca gcgcggcacg acgctcgtcc
     2641 tgacgacgca ttacatggac gaggccgagc agctgtgcga ccggctcgtc gtgatggaca
     2701 aggcgaagat cgtcgcggag ggttcaccac gcgcgctcat cgagcagtac tcgaccaagg
     2761 aggtcctgga gctgcgcttt cccgcgggcg ttcaggacac gctcgacggc cagctcgacg
     2821 gcctgggcga ccgcgtcgag aagctgcccg accgtgtcct cgtctacgcc gcggacggcg
     2881 aggccgccgc gacggccgca cacgagcgcg gcctcgtgcc agagacgatc cttgtccgcc
     2941 ggagcagcct ggaagacgtc ttcctgcgcc tcaccgggcg ctcgctgatc gagtagtcga
     3001 gccagccgca tgaccgccat cgccgcccca gcaccgcgcg gccgagcctt caacttcccc
     3061 ggcctgcgca agctcgagca ctcgctgttc gtgtaccgcc gcgtctggcg tggcacgttc
     3121 ttcggcacgc tcgtgtcgcc ggtgctgttc ctctccgcca tgggcgtggc gctgggcagc
     3181 ctcgtgccgt cgatgaccgc gttcggtggc gtgccctacc tggtgtttct cgcgccgggc
     3241 ctgctgtccg cccaggcgat gcagaccggc acgatcgaag gcacgtggcc gctcttcgcg
     3301 ggcttcaagt ggctcaagac gttcgacgcg atgatctcga cgcccatcac ggtgcgcgac
     3361 atcgtgctgg gccacctgta ctggctgttc gtgcgcatcg gcatcgtgac cggcatcttc
     3421 atggtcgtgg ccttcctatt cggcgccttc acatcaccga cgttcatcct tgcctggccg
     3481 agcgccatcc tgaccggtgt cgctttcggg atctgcatgt cggcctggac cgcgacgcgc
     3541 aagaccgagc agtccttctc gatcatcttc cgcttcatcg tcacgccact cttcctgttc
     3601 agcggcacct tcttcccgat cgagcagctg cccgaccaga tcgagtgggt ggcgtggttc
     3661 acgccgctct tccacggcgt ggcgctgacg cgcgccctgg ccattggcca tgacctcaac
     3721 ctggtcacgc tggccatcca cctggcggtc gtgctgggac tgctcgtaat cgggaccctg
     3781 ttcgcgttcc gcaccttcag aaagcgcctg gtgatctaga tggccacgac gatgaccggg
     3841 tctgctgtca cccgcgtgct gccgttcggc agccgccact ccgtccacat cctggagcgc
     3901 aaccggttca tcttccggcg tggctggatg gtcatcttca gcggcttctt cgagccgctc
     3961 ttctacctgc tgggcatcgg ctttggcctg gggtcgctga tcgggacggt tgcaggcccc
     4021 gatggaacgc ccatcccata cgcggtcttc gttgcgccgg ccctgctggc tacgtccgcg
     4081 atgaacggcg cggtctacga ctcgaccaac atgttcttcc ggctgcgcta cgagaaggcc
     4141 tacgacggca tgctcgccac gccgctcaac gtcggcgaca tcacgctcgg tgaggtgatc
     4201 tgggcgctcg gtcgcggcgc gctctacggc ctgggcttcc tcgcggtcat gttcgcgatg
     4261 ggtctcgtcc cggccgccac cggcatattc gcgctcccgg cgtcgatgct gatcggcttt
     4321 gcgacggccg cggccggact tgtcgcggtg acgttcatgc gctcatggca ggacctcgac
     4381 ctgctgttcg tcatcacgct gccactcttc ctgttcagcg gcacgttctt cccggtcagc
     4441 acctaccccg agccgctgcg caccctcgtc gagttctcgc cgttgtaccg cggcgtggac
     4501 ctggtgcgcg cgatgacgac cggcgtgatc ggcgtcgcgc aactcatcga catggtctac
     4561 ctgttcgcga tgggcctgct cttcgtgtac atcgcatccc gccgcatggg caagatgctg
     4621 ctcaaatagc gaaacgatgc ggcgcgtgac accactatcg tcacgaaact agctaaatgg
     4681 ccgtagggag cgagccctga ccgtcccttt gcgcggcgcc gccgaaatcc ctgcacgatt
     4741 ctcggtcggc catggcccga ggacgctcct gcaccccgaa atcacgcgtg aggcgcgtcg
     4801 atagctgcct cttgacgata gtggtgtcaa ggaaacgtct agataacggc gataaagcgc
     4861 tccacggcga ggtcgctcct gaacgcgcgg tcgaccagtg ccctgatccg gtagtggacg
     4921 gcgacgccct tgtcgttgac gatgcccggc gcgtcggccg ggatcgtgat ctttgtcgtg
     4981 tagccgctct tgaaggcgtt gggatccacg acctgcgaga agagcaccac gcccgacagc
     5041 ttgttcggtg cgtcgacatc ggcctggagc tccaatcggc cgccgcggcc agcgcccgca
     5101 ccgggccaat tgacggtcac ctcgaactcc gcgccggcct cgagcggcgg cagtggcttg
     5161 accgagatcg tggcgccacc gctgccgtag acgtcgaaca tggcgccgtc atcgaggcgc
     5221 accgcgccgc tgcgcaggta atccaggttc tgcttcacgt tgacgagtgc gctgacgcgt
     5281 tcatcgccgc ccatggagat gtcccacttg gcgtcgagcg cccacacgat ggcggcgctt
     5341 cccaaatggc cgctgaccgg ccccaggcgc ggggcgggca acatgaagtc cgcctcgaac
     5401 gtctggcccg cgtcgagcgt agcgggcagc ggtgtctcgg ccagctggag atcctcgaag
     5461 acgctgccgt tgatctcggt ccagcgctcg ctcttggtga cgttgccctt gctgtcgcgc
     5521 tcctcacgtg actgctcgcg ctcaacgagc agagcgccga tcaggtggaa catcgcgccg
     5581 cgcgccttga tgtccttgcg cgcggtcagc ttgatgtggc cggccagcgc ttcaccgatc
     5641 ttgaccgggt ctttgatcgt gatctcgcca tcgatgccgt tgctcgaccg cgcgacgagc
     5701 tcatgcagcc cgatcgcggc gagtggatgg ctgccctgcg gaccccacag ccgattgccc
     5761 gcgggcgcgt caaacggcgt cggcaggctg ccatgctggc ccaccccgcc aaagatgctg
     5821 aggccgttcc agccgctcac gctgttgccg ctggcgatta ccgcttcgag aaagccgggc
     5881 atttaccaat ctccgaactg cgggccttcg cgcaggccga ggacgtttcc gtcgggatcc
     5941 ttgaaccagg cgccgcgcat gcccggggcg tagttggcga tgctgttcgt cgtcgcgatc
     6001 ggaccggact cgtactcctc gaagacgacg ccgcgcgact tgaggtcagc gacggctgcg
     6061 tccatgtcgc gcacctcgaa gtgggccatc gtgtggccgc gctctacggg cggtcgcttg
     6121 aagatcgaga gctgtgtgct gcccgcgcca agacgacagg cgaacggtcc ctcgtcgagc
     6181 accgcgaggc ccagggcgtc gcggtagaag gggagcgaca cgtccagatc ggtcactggg
     6241 atcgtggcca cgatcattgc gtcgttgagc atttagggcc tcctcttggc gcggattatc
     6301 gagccaaaca gccggcccgc tccgcccgtg gcgggaagcc actcgacgga gagcgcgctg
     6361 acgaacgctg cgtagtcccg cagggcatcg cgcatggcgt caacgaggcc gacttcccgg
     6421 cgcgggctga ag
//
LOCUS       DATBRX010000234        10058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_4016690, whole genome shotgun sequence.
ACCESSION   DATBRX010000234 DATBRX010000000
VERSION     DATBRX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4016690"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /gene="nrfD"
                     /locus_tag="VIF08_08855"
     CDS             <1..571
                     /gene="nrfD"
                     /locus_tag="VIF08_08855"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY8136133.1"
                     /translation="AGLMTADVGLHTATDLLLTGPLAPEYVGLRLIGGLALPLLLILI
                     PRTRTPLGLVAAGVLALVGVFVDRLTFVIAGQAAPVTTVSGVVSAPVAQYTPSLVEIA
                     IVIAGVALVAFAYTLAERYLDLSESDVHTIIRLPPWALAGLALPGRVARRAVAVVRPI
                     RLRGRGRSRGRNAAADDGPTDPHASDSTD"
     gene            571..1368
                     /locus_tag="VIF08_08860"
     CDS             571..1368
                     /locus_tag="VIF08_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136134.1"
                     /translation="MGSLLRRYGYQLSLAVLVGLIGLGGVFVLVVVLPDMQAANVAPT
                     ATPSVTPTAPSVVMSWVNLPPDADCAACHQTQSGVGVVAVPRIAHPLRGWTDCTACHA
                     NDRLVTTAPGHGGIHATDCLVCHEPAELPAPLSRPHRELQNQECLSCHGITAPLPEDM
                     AHRTESVCWLCHRLPDEQPPAPRHAIFPGQSDCLACHVAADYGALPDDHLSRTANECV
                     LCHLPGPGATVEPSATSLPGATSGLGESASPALQQGIRFPLVFVRPI"
     gene            1501..1758
                     /locus_tag="VIF08_08865"
     CDS             1501..1758
                     /locus_tag="VIF08_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136135.1"
                     /translation="MAIVRVTPTEVDVKTGMFDGRPRHIRIANERVPVLEISQVRDEL
                     SAYPVALGPRRIFDIRTPDARLRLSFGKRHRRWLLEGLEAA"
     gene            1795..2460
                     /locus_tag="VIF08_08870"
     CDS             1795..2460
                     /locus_tag="VIF08_08870"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEY8136136.1"
                     /translation="MDTAPSDSLAELTLTQFSDRLASAEPVPGGGSASAIAGSFAGSL
                     LAMVARLSADRPKYAAYQKTHGRALATGERGRTVLLELADADARAYAGFAAARKMPKE
                     TDAEQRARDEATRVAARASTEVPLSVVSECALLLDDVVAMAGRSNVNAASDLEVASRL
                     AAAAARGAAANVLINLPMVADEDYAAATTESVSGLLDTIERNAALVTEIVGSGELREP
                     EQE"
     gene            2457..3389
                     /locus_tag="VIF08_08875"
     CDS             2457..3389
                     /locus_tag="VIF08_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY8136137.1"
                     /translation="MTTAPAAVLRYDEPARILDGRPLAARIRAGVKRQIAAFDRSYGF
                     RPQLAAIIVGHQAASNVYVQQILRSCAAVGIPSRVLELPRHTTADELRKRLEKLNRDA
                     EVAGIIVQQPLPRHIPLSTLTDTIMPAKDIDGIHPLNTGLMTLGYEGFLPACAEAAVE
                     IPKFYGYNLEGKRAVVIGRSNVVGKPVQLLLMREHCTVTVCHRRTRDLAAEIARAEIV
                     VCAAGSAGLVNGKMLHPGALVVDVGINVVSGRVVGDVDFDSALRVAAAITPVPGGVGP
                     VTNAVLLEHLVRAARWQQTDHPIIAGHARKRRAA"
     gene            3413..5104
                     /locus_tag="VIF08_08880"
     CDS             3413..5104
                     /locus_tag="VIF08_08880"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEY8136138.1"
                     /translation="MQSSLDIARAAKLRPIVDVAHELGLRDDELDLFGTYKAKIQPSA
                     IERLSDRPDGREIVVTAITPTPLGEGKTTTTIGLAQGLNRIGVRAAPTIRQPSLGPVF
                     GLKGGAAGGGYSQVLPMEDINIHFTGDLHAVAAAHNLGAAFLDNHIFRGNSLGIDPRT
                     ARWPRVIDVNDRALRRVMIGLGDEKALRETEWHITAASEVMAILALASDIHDLRARIG
                     RCIVGETFEGEPVTLEQLDVAGAMTVLLREAIKPNLVQTLEGGPAFIHAGPFANIAHG
                     NSSVAADRIALKLVDAVVTEAGFGADMGFQKFVDIKCRLSGIRPAAAVIVATIRALKM
                     HGGVGHVVAGRPLDEALMREDPEGVRRGCENLAQHVEIVRGYGIPAVVAINAFPTDKP
                     SEVAVVREAALAAGARAAVVTDHFQRGGAGAEELARAVWDAARSDGEEFRFLSRDEAT
                     LEERITRIATRVYGADGVDISPEVGKALHEFERLGYGQLPVCMAKTQLSLSHDPALKG
                     RPKGFRVPIRDARLFAGAGFVTAYCGDMLMMPGLPSHPSGEDVDINEAGETIGLF"
     gene            complement(5120..5329)
                     /locus_tag="VIF08_08885"
     CDS             complement(5120..5329)
                     /locus_tag="VIF08_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136139.1"
                     /translation="MTDQHASDHDAVAEHDPVTHMDAHTAISDDDHGHAEGSLGPIDW
                     GAWGLAILGGASGVLVLLCFWVALN"
     gene            complement(5326..6294)
                     /locus_tag="VIF08_08890"
     CDS             complement(5326..6294)
                     /locus_tag="VIF08_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8136140.1"
                     /translation="MSVVLLAVGCGSGTPTTTPLTKLTVGLGYIPSVQFAQFYRAKVQ
                     GYYEAAGLDVTFQNGDDANLITLIAQGANDIGIADGTSVIPAVGQGIPIRYVATMYAR
                     FPNVVITGADSGINTVADLRGRSIGIPGPYGSSWIMLQALLMSENMTTADIVERDYPD
                     YGQGVALQQGQVNAATGFRNNEPVQLALAGFETVQFAVDEIVPLPGPGLIVGTSTLAS
                     KRDALRAFVAATLRAMREIDESPEAGLEDAIAVVPTLASNRETQLAILRATIEMWHSP
                     ATDANGLGAIDEGGWSSSLSFMRELPDSNIPGTVTVDDLISTELLP"
     gene            complement(6321..7076)
                     /locus_tag="VIF08_08895"
     CDS             complement(6321..7076)
                     /locus_tag="VIF08_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8136141.1"
                     /translation="MRTRSTLLPIIAAAIVFVVVWKLVVVIGNYPTFILPSPELVAQR
                     FVEAWSDGTMWPHAWATLVEIGLGFVVGASLAVGVGYLLARSRLAERLLSPYLVAAQA
                     TPVLVLAPLLVLWLGTGLLPKIVICALIVFFPIAVATMVGIRSVDRRLLELGRSLRAT
                     PTQVLLKLELPAALPQILGGMRVGVTLAVIGAIVGEWAGADRGLGVLINLARGSLFDF
                     PLMFATLATIALIALTLYLVVLLVERALVGAWR"
     gene            complement(7076..7876)
                     /locus_tag="VIF08_08900"
     CDS             complement(7076..7876)
                     /locus_tag="VIF08_08900"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8136142.1"
                     /translation="MTVLDDVSFEVPVHGICAVVGPNGSGKSTLLRLVCGLLTPDSGT
                     VEIAGVPVADADQRVGLVFQEPRLLPWRTALDNVAFPLELSGVTRAERHERARSLLDL
                     VGLAQFARAYPHQMSGGMRQRAAIARALAREPQILLLDEPFSALDALTRERFNAELLD
                     IWQRTGTTILIVTHSIAEAVFLADEAVVLTDRPGRVAALVPIPLGRPRELDALDSAAF
                     TRAAATIRAHLSGSPDDIAAQEAAAAPVRDVLERAGAPAWFDPFRGAS"
     gene            8163..8816
                     /locus_tag="VIF08_08905"
     CDS             8163..8816
                     /locus_tag="VIF08_08905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY8136143.1"
                     /translation="MLTLLLTRHGHTDRSEPEQYLGQRINAELSARGRADARKLARRL
                     ANVEIQRVVTSPLKRAHETADILARAHGIAVEPEPRLVELDYGDWEGHTLEDIYALFP
                     GEYEKYDRDPAHHEVGGGESGTEVAARLWPVIDGLIDWAEKRGGESTAVVVGHSSTNR
                     ILLAMLLGVELNDYRRRFDADWTSLTVLRWPDRASGPRLLLSNDQAHTRGMRGVTWG"
     gene            8826..>10058
                     /gene="hutH"
                     /locus_tag="VIF08_08910"
     CDS             8826..>10058
                     /gene="hutH"
                     /locus_tag="VIF08_08910"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY8136144.1"
                     /translation="MDQLSLTGSDLTLDDVWRVAHDGHPVVLAPEAAERMRASRAVID
                     ELVASDAVVYGVTTGFGDLVSKRISAADSARLQENLLMSHAVGVGPAHGKHTVRAMLL
                     LRANALARGFSGVRPEVVERLLDFLREEIYPVVPEQGSVGASGDLAPLAHLALPLIGR
                     GRAEVDGEVLDGAAALAKRGLAPLTLAAKEGLALLNGTQQMTAIGALVLMHAESLLQT
                     ASVAAAMSVEALMGTDVAFSDALQQTRPHPGQVAVARQMRHLLRDSALIKSHHAGAHK
                     IQDPYSIRCVPQVHGAAWDALAHVRRVLEIEINAVTDNPLVFPDGADVDPTAAATGGG
                     RVVSGGNFHGEPIAMAMDFMKIAVAEIGSISERRIAQLVDAKSSGLPPFLVKDAGLNS
                     GLMLFQYTAAALVSENKVL"
BASE COUNT         1606 a         3457 c         3443 g         1552 t
ORIGIN      
        1 ggctggcctg atgaccgccg acgtcggcct gcatacggcc actgacctgc tgctgaccgg
       61 accattggcg cccgagtacg tcggcctgcg cctgatcggc ggacttgcgc tgcccttgct
      121 attgatcctg atacctcgga cgagaacacc gcttggcctc gtcgcggccg gagtgttggc
      181 tttggtcggc gtcttcgtcg atcggctgac cttcgtcatc gccggtcagg cggcacccgt
      241 gaccaccgtg tcaggcgtgg tttcagctcc agtggcccag tacacgccat ccctggtgga
      301 gatcgcgatc gtgatcgcag gagtggcgct ggtcgcgttt gcctacaccc tcgccgagcg
      361 ctacctcgat ctctccgagt ccgacgtgca cacgatcatc cgcttgcctc cctgggcgct
      421 cgccgggctc gccctgccgg gccgcgtggc gcgcagggcc gtcgccgtgg tgcgcccgat
      481 ccgtttgcgg ggacgcggtc gcagtcgagg tcgcaatgca gctgcggacg atggaccgac
      541 cgatcctcat gcatcggatt cgaccgactg atgggcagcc tgctgcgccg ctatggctac
      601 cagctaagcc tcgccgtcct ggtcgggctg atcggcctgg gaggcgtgtt cgtactcgtg
      661 gtcgtcctgc ccgatatgca ggccgccaac gtggcaccga ccgcgacgcc gtcggtgacg
      721 cccaccgcgc cgagcgtggt catgtcgtgg gtcaatctgc cgcctgacgc cgattgtgcg
      781 gcgtgtcacc agacccagag cggcgtgggc gtcgtcgccg tcccacgcat cgcccatccg
      841 ctgcgcggct ggaccgactg cacggcgtgt catgccaacg atcgcctggt cacaacggct
      901 cccggccacg gcggcatcca cgccaccgat tgcctggtct gtcatgagcc ggccgagctg
      961 cccgccccgc tatcgcgacc acatcgcgag ctgcagaacc aggaatgtct cagttgccat
     1021 gggattacgg cgccgctgcc ggaggacatg gcacaccgca cggagtcggt gtgctggcta
     1081 tgccaccgcc tgccggacga acagccgccg gccccccggc acgcgatctt tcccggccag
     1141 tccgactgcc tggcctgcca cgttgccgcc gactacggcg cgctgccgga cgaccacctg
     1201 tcacgcaccg cgaacgaatg cgtgctctgc cacctgcccg ggccgggcgc cacggtcgag
     1261 cccagcgcta cctcattacc tggcgctacg tccggacttg gcgagtccgc atcccccgcg
     1321 ctacagcagg gcatccgctt tccgctggtt ttcgttcgcc cgatctgacg gtcgtgacgc
     1381 gctagctggc acgcggctcg cgaagactgg ctccagccac cccggtggtg ggtgttgaca
     1441 atcgaacgcc tgttcgatac gttgtgtgaa cagatgttct atgccgtgga ggcaagctag
     1501 atggcaatcg tgcgagttac cccaacagag gtcgacgtga agaccggcat gtttgacggt
     1561 cggccgcgtc atatccgtat cgccaacgag cgcgtgccgg tgctcgagat cagccaggta
     1621 cgcgacgagc tgtcggcgta tccagtggca ctcggaccac gtcgcatctt cgacattcgg
     1681 acgccggacg cgcgtctccg cctgtcgttc ggaaagcgcc accggcgctg gctgctcgag
     1741 ggtctcgagg ccgcctaacc tccaatcagt cgcagcgatg cgacaatcgc ggccgtggac
     1801 accgcgccat ccgactccct tgccgagctg acgctcactc agttctcgga ccgcctcgcg
     1861 tcggctgagc cggtgccggg cggtggcagc gcgtcggcca tcgcgggcag cttcgcgggg
     1921 agcctgctgg ccatggtcgc gcggctcagc gccgaccggc ccaagtacgc ggcttatcag
     1981 aagacgcacg gccgcgcgct tgcgaccggg gaacgcggcc gtacggtcct gctcgagctg
     2041 gccgacgccg acgcgcgcgc ctacgcgggc ttcgccgcgg cccgcaagat gcccaaggag
     2101 acagacgctg agcagcgggc ccgcgacgag gcgacccggg tggcggccag ggcttccacc
     2161 gaagtgccac tatcggtggt gagcgaatgt gcgctgctgc tcgatgacgt cgtcgcgatg
     2221 gcggggcgca gcaatgtcaa tgcggcaagc gacctcgagg ttgcttcgcg cctcgcagcc
     2281 gccgcagcac gcggcgcagc agcgaacgtg ctgatcaacc tgcccatggt cgctgacgag
     2341 gactatgcgg ccgcgacgac ggaatcggtc agtggcctgc tcgacacaat cgaacgcaac
     2401 gcggcgctcg tcacggagat cgtcggcagc ggcgagttgc gcgagcccga gcaggagtga
     2461 ccaccgcgcc ggcagctgtg ctgcgctacg acgagccggc gcgcattctc gacgggcgac
     2521 cactcgcggc ccgcatccgc gccggagtca aacgccagat cgccgcgttc gataggtctt
     2581 atggattcag gccgcagctc gcggccatca tcgtcggcca ccaggccgca tcgaacgtct
     2641 acgttcagca gatccttcgt tcgtgcgcgg cggtgggcat accaagccgc gtactggagc
     2701 tgccgcgcca cacgaccgca gatgagttgc gcaagcgtct cgagaagctc aaccgcgacg
     2761 ccgaggtagc gggcatcatc gtccagcagc cactgccgcg gcacatcccg ttgagcaccc
     2821 tgaccgacac gatcatgccg gccaaggaca tcgacggcat ccatcctctc aacaccggct
     2881 tgatgacgct cggttacgag ggtttcctgc ccgcctgcgc ggaggctgcg gtggagatcc
     2941 ccaagttcta cggctacaac ctcgagggca agcgcgcggt cgtcatcggg cgcagcaatg
     3001 ttgtcggcaa gcccgtgcag cttctgctca tgcgcgaaca ctgcaccgtg accgtgtgcc
     3061 accgtcggac gcgcgacctc gcggctgaga ttgcgcgggc agagatcgtc gtctgcgccg
     3121 cgggctcggc cggtctggtc aacggcaaga tgcttcatcc cggcgcgctc gtggtcgatg
     3181 tcggcatcaa cgtcgtcagc ggccgggtgg tgggcgacgt cgacttcgac tccgcgctgc
     3241 gcgtcgccgc cgccatcaca ccggtgcccg ggggcgttgg tccggtgacc aatgcggtcc
     3301 tgctcgagca cctcgttcgc gccgcgcgct ggcagcagac cgaccatccg atcatcgccg
     3361 gccacgcgcg caagcgccgc gccgcgtagc ccgcagtccg cagcgacgcc gcatgcagtc
     3421 aagcctcgac atcgcccgcg ccgcaaagct gcgccccatc gttgatgtcg cgcacgagct
     3481 cgggctgcgc gacgatgagc tcgacctgtt cggcacgtac aaggccaaga tccagcccag
     3541 cgcgatcgag cggctcagcg accgaccgga cggcagggag atcgtcgtca ccgcgatcac
     3601 ccccacgcca ctcggcgagg gcaagacgac caccacgatc ggcttggccc agggtctgaa
     3661 ccgcatcggg gtgcgcgccg cgccgaccat ccgccagcca tcactcggcc cggtgttcgg
     3721 tctgaagggc ggcgcggccg gtggcggcta cagccaggtc ctgcccatgg aggacatcaa
     3781 catccacttc acgggcgacc tgcacgcggt cgccgccgcg cacaacctgg gcgccgcgtt
     3841 cctcgacaac cacatttttc gcggcaactc gctgggcatc gacccacgga cggcccgctg
     3901 gccgcgagtc atcgacgtca acgaccgtgc cctgcgccgg gtgatgatcg ggctgggcga
     3961 cgagaaggcc ctgcgcgaga ccgagtggca catcaccgcc gcgtcagagg tgatggcgat
     4021 tctcgccctg gcgtcggaca tccatgacct gcgcgcgcgt atcgggcggt gcatcgtcgg
     4081 cgagacgttc gagggcgagc ccgtgacgct cgagcagctc gatgtcgccg gggccatgac
     4141 ggtgctgttg cgcgaggcca tcaagcccaa tctcgtgcag acgctcgagg gcggcccggc
     4201 gttcatccat gccggtccgt tcgccaacat cgcccacggc aattcgtcgg tcgcggcgga
     4261 tcgcatcgcg ctcaagctgg tcgacgcggt cgtgaccgag gcgggctttg gtgccgacat
     4321 gggcttccag aagttcgtcg acatcaagtg ccgcctctct ggcatcagac cggccgcggc
     4381 cgtcatcgtc gccactatcc gcgcgctcaa gatgcacggc ggtgttggcc acgtggtggc
     4441 gggcaggccg ctcgacgagg cgctcatgcg cgaggatccg gaaggtgtgc ggcgcggctg
     4501 cgagaacctc gcgcagcacg tggagattgt gcgcggctac ggcatcccgg cggtcgtcgc
     4561 gatcaacgcc ttcccgaccg acaagccgag cgaagtggcc gtagtgcgcg aggccgcgct
     4621 ggccgcgggt gcccgcgccg cggtggtgac cgatcacttc cagcgcggcg gcgcgggcgc
     4681 cgaggagctg gcacgcgcgg tttgggacgc cgcgcggagc gacggcgagg agttccgctt
     4741 cctgtcacgc gacgaggcca cgctggagga acgcatcacg cgcatcgcga cccgcgtgta
     4801 cggcgcggac ggcgtcgaca tctcaccgga agtaggcaag gcgctgcacg agttcgagcg
     4861 cctgggctac ggccagctgc cggtgtgcat ggccaagacc cagctttcac tcagccacga
     4921 cccggcgctc aagggccggc ccaagggctt ccgcgtaccg attcgtgacg cgcggctgtt
     4981 tgccggggcg ggattcgtga ccgcctactg cggcgacatg ctgatgatgc ccggcctgcc
     5041 gtcccatccg tcgggcgagg acgtcgatat caacgaggcc ggcgagacga tcggcttgtt
     5101 ctaggtcctt gtctgtctgc tagttcagcg ccacccagaa gcagagcagc accaggacgc
     5161 cggacgcgcc gcccaggatc gccaggcccc acgcgcccca atcgatcggg ccaagggagc
     5221 cctcggcatg accgtggtcg tcgtcactga tggcggtgtg cgcgtccatg tgcgtgaccg
     5281 ggtcgtgctc ggcgactgcg tcgtggtcgg aggcgtgttg gtcggtcacg gcaggagctc
     5341 cgtactgatc agatcgtcga cggtgactgt accggggatg ttcgagtccg gcagttccct
     5401 catgaagctg agcgaagagc tccagccgcc ctcgtcgatc gcgccgagac cgttcgcgtc
     5461 cgtcgccggc gagtgccaca tctcgatcgt cgcgcgcagg atcgccagtt gcgtctcgcg
     5521 gttggaagcc agcgtgggca ccaccgcgat cgcgtcttcg agtccggcct ccggtgactc
     5581 gtcgatctca cgcatggcgc gcaatgtcgc ggccacgaag gcgcgcaggg cgtcgcgctt
     5641 ggaggccagc gtgctcgtgc cgacgatcag ccccgggccg ggcagcggca cgatctcgtc
     5701 gaccgcgaac tggacggtct cgaaaccagc gagcgcaagc tgcactggct cgttgttgcg
     5761 gaagccggtc gcagcgttca cttggccctg ctggagtgcg acaccctggc cgtagtcggg
     5821 atagtcgcgc tcaacgatgt cggcggtcgt catgttctcc gacatcagca atgcctggag
     5881 catgatccac gacgagccat acgggcccgg gatgccgatg ctgcgaccgc gcaggtcggc
     5941 gaccgtgttg atgccgctgt ccgcgccggt gatgacgacg ttcgggaatc gcgcgtacat
     6001 cgtcgccacg tagcggatgg gaatgccctg gcccacggcc gggatgacgc tcgtcccgtc
     6061 agcgatgccg atgtcgtttg cgccctgggc gatgagcgtg atcaggttgg cgtcgtcgcc
     6121 gttctggaac gtgacgtcca ggcctgccgc ctcgtaatag ccctgcacct tggcgcggta
     6181 gaactgcgcg aactggacgc tgggtatgta gcccaggcca accgtcagct tggtcaacgg
     6241 cgtggtcgtc ggcgtgccag agccgcaacc gactgcgagc aggactaccg acaagacggc
     6301 caggcacaag cgacgcacgt tcacctccag gcgcccacga gggcgcgttc gaccagcaag
     6361 acaaccaggt agagcgtcaa ggcgatcagc gcgatggtcg cgagggtcgc gaacatcagc
     6421 ggaaagtcga agagcgagcc gcgggccagg ttgatcagta cgcccaggcc acggtcggcg
     6481 ccggcccact cacccacgat cgcgccaatc accgcgaggg tgacgccgac gcgcatgccg
     6541 cccaggatct gcggcagcgc ggcgggcagc tcgagcttca gcagcacctg tgtgggcgtg
     6601 gctcgcaaac tgcggcccag ctccagaagc cggcgatcaa ccgaccgtat gccgaccatc
     6661 gtcgcgaccg cgatcgggaa gaagacgatc agggcacaga tcacgatctt gggcagcagc
     6721 ccggtgccca gccagagcac gagcagcggt gcgaggacga gcaccggcgt tgcctgcgca
     6781 gcgacgagat acggcgagag cagccgctca gcgagacgcg aacgggcgag caggtagccg
     6841 acgcccaccg ccagactggc gccgaccacg aagccaagcc cgatctcgac cagcgttgcc
     6901 caggcatgcg gccacatcgt cccgtcagac caggcctcga cgaatcgctg cgcgaccagt
     6961 tccggtgacg ggaggatgaa ggtggggtag ttgccgatga ccacgacgag cttccagacc
     7021 acgacgaaca cgatggccgc ggcgatgatc ggcaggagag tcgatcgcgt gcgcatcagc
     7081 tcgcaccacg gaatggatcg aaccacgccg gtgcgccggc gcgctccagt acgtcgcgca
     7141 cgggtgccgc cgcggcctcc tgcgctgcga tgtcgtcggg cgacccggac aggtgcgcgc
     7201 ggatggtcgc ggccgcgcgc gtgaaggccg cggagtcgag cgcgtccagc tcacgcggcc
     7261 ggccgagcgg gatcggcaca agcgcggcga cgcgaccggg ccggtccgtc aacacgaccg
     7321 cctcgtcggc caggaacacg gcctcggcga tgctgtgggt gacgatcagg atggtcgtgc
     7381 cggtgcgctg ccagatgtcg agcagctcag cgttgaagcg ctcgcgtgtc agggcgtcaa
     7441 gagcgctgaa cggctcgtcc agcagcagga tctgcggctc ccgggcgagc gcgcgcgcga
     7501 tcgccgcgcg ctggcgcatg cccccggaca tctggtgcgg gtaggcgcgc gcgaactggg
     7561 ccagacccac gagatcgagc agcgagcgcg cgcgctcgtg gcgctcagcc cgggtcactc
     7621 cgctcagctc gagtgggaac gcgacgttgt cgagcgcggt gcgccagggc agcagccgcg
     7681 gctcctggaa cacgagcccc actcgctggt ccgcgtcggc aacgggcacg ccggctatct
     7741 cgaccgtgcc cgaatcgggg gtgagcagcc cacagacgag ccgcagcagc gtcgacttgc
     7801 cgctgccgtt ggggcccacg actgcgcaga tgccatgtac cggcacttca aatgagacgt
     7861 cgtccagcac agtcacgggc cgtccgtcga tgacgaagcg gcgcgcgacg tgggcgagtt
     7921 tgatcgcggg aatgcgttcg actgtcagat gtcctccctt ctcccatccg gactcttacc
     7981 gtcggctgcc tgccagggca tccaccttcg ccgcgtccgc gccgttgccg gcccagacgg
     8041 ggctccgggt cgtgggctgg tccgtaccga tcactcgatc gtcacgggcc gaccaccggt
     8101 agggaattgc accccacccc gaaggaaagg cgtattcgat tggcctcaag gatacttcgc
     8161 gcgtgcttac tctccttctg acgcgccacg gccacaccga tcgctccgag cccgagcagt
     8221 acctggggca gcgcatcaac gccgagttga gcgcgcgcgg acgcgcggac gcgcggaagc
     8281 tcgcccgccg gctggccaac gttgagatcc agcgtgtcgt gacgagcccg ctgaagcggg
     8341 cgcacgagac ggccgacatc ctcgcgcggg cgcatggcat cgcggtggag cccgagccgc
     8401 gcttggtgga gctcgactac ggcgattggg agggccacac gctcgaggac atctacgccc
     8461 tcttccccgg tgagtacgag aagtacgacc gcgacccggc gcaccacgag gtgggcggcg
     8521 gcgaaagcgg gacggaggtc gccgcgcgtc tctggccggt catcgacggg ctgatcgact
     8581 gggccgagaa gcggggcggg gaaagcaccg cggtggtggt cggccattcg tcgaccaacc
     8641 ggatcctgct ggcgatgctc ctgggcgttg agctgaacga ctaccggcgg cgcttcgacg
     8701 cggactggac gagcctgacc gtgctgcgct ggcccgaccg cgcgtcgggg ccgcgcctgc
     8761 tgctgtcgaa cgaccaggcg cacacgcgcg gcatgcgcgg cgtgacctgg ggttaggctc
     8821 acggcgtgga ccagctcagt ctcaccggct ccgacctgac gctcgacgac gtctggcgcg
     8881 tggcccacga tggccacccc gtcgtgcttg ctccggaagc ggcggagcgg atgcgcgcca
     8941 gccgcgccgt aatcgacgag ctcgtcgcgt ctgacgccgt ggtctacggc gtcacgaccg
     9001 gcttcggcga tctcgtttcc aagcgcatct ccgcggccga ctcggcacgc ctccaggaaa
     9061 acctgctgat gagccacgcc gtgggcgttg gtccggctca cggcaagcac accgtgcgcg
     9121 ccatgttgct gttgcgcgcc aatgccctgg cgcgcggctt cagcggcgtg cgacccgaag
     9181 tcgtcgagcg gctgctcgac ttcctgcgcg aggagatcta tccggtcgta cccgagcagg
     9241 gctcggtcgg tgcgtcgggt gacctggcgc cgctcgccca cttggcgctg ccgctgatag
     9301 gccgcggccg ggcggaggtc gacggcgagg tgctcgacgg cgcagctgcc ctggccaagc
     9361 gtggcctggc gccgctgacg ctggcggcca aggaaggtct ggcgctgctc aatggcacgc
     9421 agcagatgac ggccatcggc gcgctggtgc tgatgcacgc ggaatcgctt cttcagacgg
     9481 ccagcgtcgc ggcggcgatg agcgtcgagg ccttgatggg cacggacgtt gcgttctcgg
     9541 acgcgctcca gcagacgcgg ccccatcccg gtcaggtcgc ggtcgcgcgg cagatgcgcc
     9601 acctgctacg cgattcggcg ttgatcaaat cgcatcacgc gggcgcgcac aagatccagg
     9661 atccgtactc catccgctgc gtcccgcagg tccacggcgc ggcttgggac gcactcgccc
     9721 atgttcggcg cgtgctcgag atcgagatca acgccgtcac cgacaacccg ctcgtctttc
     9781 ctgacggggc ggacgtcgat cccactgcgg ccgcaaccgg cggcggcagg gttgtgagcg
     9841 gcggcaactt ccacggcgag ccaatcgcga tggcaatgga cttcatgaag atcgccgtcg
     9901 ccgagatcgg ttcgatcagc gagcggcgga tcgcgcagct cgtggatgcc aagtcgtcgg
     9961 gcctgccgcc gttcctggtg aaggacgccg gcctgaacag cggcctgatg ctcttccagt
    10021 acacggccgc ggcgctggtg agcgagaaca aggtgctc
//
LOCUS       DATBRX010000235         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2565509, whole genome shotgun sequence.
ACCESSION   DATBRX010000235 DATBRX010000000
VERSION     DATBRX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2565509"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VIF08_08915"
     CDS             complement(<1..318)
                     /locus_tag="VIF08_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8136145.1"
                     /translation="MTLIRRTNPFGELISLRAAMDRLFEDSWVRPHNSAADEHPLALD
                     VRTTNEAYEIEAALPGVKPEDVDISVLGETLTITARTGQEQSRADDGYSYREIRRGSF
                     SRTV"
     gene            complement(486..1250)
                     /locus_tag="VIF08_08920"
     CDS             complement(486..1250)
                     /locus_tag="VIF08_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136146.1"
                     /translation="MGAAGVLRRSLLIWGWGHIALGDRRGWLLVLLQPLAIAGLAVAA
                     WQLIDGTRWLAVFLPLVALLVLWVGQAVHAHRRALAMGAAPGGELQLVWFLPAVLTVV
                     SAFWLVGGRHASPASAVEAYMTAWQGDRPDIAVALYGDGGPAAADVAQLWVGERSWLS
                     SQIEEARATYGPASGLDPARPFSSLRVSQRGPTSFIVELVRAETYQTTILGFIPTAGQ
                     RTVVIAPVLALTVVEKTVTGYLPTSVWRIETVTTAH"
     gene            complement(1289..1975)
                     /locus_tag="VIF08_08925"
     CDS             complement(1289..1975)
                     /locus_tag="VIF08_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY8136147.1"
                     /translation="MYIIIVGGGKVGYYLAKELLEAGHELVILEKNAGRARQIADELG
                     SIVLNRDGCEGKYLAEAGANRAAIICAVTGDDDDNLVVCQMAKHHFDVPRTIARVNNP
                     RNEDLFHHLGVDEIISPTRMALASIEQDIPVHELLHLAQLKGGEMELVEAQIAEGSPA
                     LGRKPGDINLPEGSSLFLLLRGNNVQQVRPDTVFQAGDKVLAVAKGETGEDVLRRELI
                     GDSEPVGSAS"
     gene            complement(1979..2491)
                     /locus_tag="VIF08_08930"
     CDS             complement(1979..2491)
                     /locus_tag="VIF08_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY8136148.1"
                     /translation="MRAVIVGCGRVGAGLAERLARAGHDVTIIDLNSDAFGRLEPDFP
                     GQAIRADGTDEDVLRRVGTDTADWFYALTEGDNRNAFTAQMAAESFGVKNVVAKINDP
                     VRAEAYTDLGIATICRTRLLVDALARYSGLPADPGADGVRQAERPHDHAVPSTATGLG
                     NRVEPLEQGS"
     gene            complement(2527..3039)
                     /locus_tag="VIF08_08935"
     CDS             complement(2527..3039)
                     /locus_tag="VIF08_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8136149.1"
                     /translation="MPEGANPNPIRKAVLGLNGGPTDQLVVNLGCQLAKLHSAELVAI
                     HVIEVNWQHELSDEMTDENLAASSVLDVAEGIAERYKIKLSGDLLQAREVAAALVDEA
                     IELGADAIIVGLPYRKRFGGDFAMGTTIPYILQNAPCQVIVAREPVPATGERRDNRQE
                     LTAGGGAGLR"
     gene            complement(3054..>4025)
                     /locus_tag="VIF08_08940"
     CDS             complement(3054..>4025)
                     /locus_tag="VIF08_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8136150.1"
                     /translation="LFYLYQAFTALILFLAANTSFNAFPRLAAILARDGFMPRVFSFR
                     GDRLAFTSGIVILSVAAITLIVAFGGVTTALIPLYAVGVFMAFTIGQLGLVRHWLAER
                     SAGWRKKMSINAFGAALTGVVFFVVLVAKAPTSLLVTIIIPALILIMVFIHRQYTASA
                     NQLAMSEEAVIPPPRREERVIVPVPGINRAVVQAVNVARSIAPDIRAVLISDEPEEAA
                     RMRERWEHQLPQVPLVIVESPYRALVGPMLAYLDVLDKTWPEDKEDPITFVVIPEYVA
                     RHWWERILYNQSANRLRSALLGRPHTVVVNVPYRREEDERAAAPPRG"
BASE COUNT          649 a         1357 c         1382 g          637 t
ORIGIN      
        1 gactgttcga ctgaacgagc cgcggcgaat ctcgcggtac gaatagccat cgtccgcgcg
       61 cgactgctcc tggcccgtcc gcgcggtgat ggtcagcgtc tcacccagga cggagatatc
      121 gacgtcctcg ggcttcacgc cgggcagagc ggcctcaatc tcgtacgcct cgttcgtcgt
      181 gcgcacgtcg agcgcgagtg gatgctcgtc cgcggcgctg ttgtgcggcc gaacccacga
      241 gtcctcgaac agtcggtcca tcgctgcccg cagtgagatc agctcaccga atggattggt
      301 gcgtcgaatc aaggtcatgg cacacctccg catgcctgga atctgttgtt tgatggccta
      361 cctgccgcgt ttttggcggc atcgggcgat aacctaaacc ctgacaatgg cattgtcaag
      421 acacctgctg tctgttggga agatgtgttg ccaccgggcg acaccgcccg cgcgccagga
      481 tcggatcagt gggcggtcgt caccgtttcg atccgccaaa cggaggtcgg aagatagccg
      541 gtcactgttt tctcgacgac ggttagcgcg agcaccgggg cgatgaccac cgtgcgctga
      601 ccggccgtcg ggatgaagcc caggatggtc gtctggtagg tctcggcgcg caccagctcc
      661 acgatgaagg acgtcggccc cctctggctg acgcgcaggc tgctgaacgg tcgcgccgga
      721 tcgaggccgc tcgccggccc gtaggttgcc cgggcctctt cgatctggga gctcagccac
      781 gaccgctccc cgacccagag ctgggccacg tccgcggcag cgggcccgcc gtcgccgtac
      841 agggcgacgg cgatatcggg ccggtcccct tgccatgccg tcatgtaggc ctcgaccgct
      901 gaggccggcg acgcgtggcg gccgccgacc agccagaacg cgctgacgac cgtcaggaca
      961 gcaggcagga accagaccag ctgcagctcg ccaccgggcg cggcacccat ggccagcgcg
     1021 cgccggtggg catgcaccgc ctggccaacc cacaagacca gcagcgcgac cagcggcagg
     1081 aacaccgcga gccagcgcgt gccgtcgatc agctgccagg ccgcgaccgc caagccggca
     1141 atggcgagcg gttggagcag cacgagcagc cagccgcgcc tgtcgccaag cgcgatatgg
     1201 ccccagcccc agatgagaag ggagcggcgc aggacgccgg ccgcgcccac gggggcgcga
     1261 cgatcagacg tcgtgatcgt cccccgtgct agctggctga gccgaccggc tcgctgtcgc
     1321 cgatcagctc gcgccgcagg acatcctcgc ctgtctcacc cttggccacg gcgagcacct
     1381 tgtcgcccgc ctggaacacc gtgtcgggtc gcacctgttg gacgttgttg ccgcgcagca
     1441 gcaggaagag cgacgagccc tcgggcaggt tgatgtcgcc gggcttgcgc ccgagtgccg
     1501 gcgacccctc cgcgatctgc gcctcgacca gctccatctc cccgcccttg agctgcgcca
     1561 ggtgaagcag ctcgtggacc gggatgtcct gctcgatcga tgccagggcc atgcgcgtcg
     1621 ggctgatgat ctcgtcgacg cccaggtggt ggaagaggtc ctcgttgcgc gggttgttga
     1681 cgcgcgcgat ggtgcgcggc acatcgaagt gatgcttggc catctggcac acaacgaggt
     1741 tgtcgtcgtc gtcgccggtc accgcacaga tgatcgccgc gcggttggcg ccggcctcgg
     1801 cgaggtactt gccctcgcag ccgtcgcgat tgaggacgat gctgccgagc tcgtccgcga
     1861 tctggcgcgc tcggccggca ttcttctcga ggatcaccag ctcatggccg gcctcgagca
     1921 gctccttggc gaggtaatag cccaccttgc cgccgccgac gatgatgatg tacacgggct
     1981 agctcccttg ctcgagcggc tcgacccgat tgcccaggcc agtggcggtc gatggcacgg
     2041 catgatcatg gggtcgctct gcctggcgga cgccgtcggc gccaggatcg gcgggcaggc
     2101 ccgagtaccg cgccagcgcg tcgaccagca gccgcgtgcg gcagatggtg gcgatgccca
     2161 ggtcggtgta ggcctccgcg cgcactgggt cgttgatctt ggccacgacg ttcttgacgc
     2221 cgaagctctc agccgccatc tgggccgtga acgcgttgcg gttgtcgccc tcggtcagcg
     2281 catagaacca atcggccgtg tcggtgccga cccgacgaag cacgtcctcg tccgtgccgt
     2341 cggcacgaat cgcctggccg ggaaagtcgg gctccagccg tccgaacgcg tcggagttga
     2401 ggtcgatgat ggtcacgtcg tggcccgcgc gagccagccg ctcggccagc ccggcgccaa
     2461 cccggccgca gccgacgatc acagcgcgca cttggaagtc gcctccattg atgttcaggc
     2521 gtgagcctag cgcaagccgg cgccgccgcc tgcggtcagc tcctggcggt tgtcgcgccg
     2581 ctcgcccgtg gcgggcaccg gctcgcgcgc gacgatcacc tggcagggcg cgttctggag
     2641 gatgtagggg atggtcgtgc ccatcgcgaa atcgccgccg aagcgcttgc ggtagggcag
     2701 gccgacgatg atcgcgtcgg cgcccagctc gatcgcctca tccacgagcg cggcggcaac
     2761 ctcgcgcgcc tgtagcaggt cgcccgagag cttgatcttg tagcgttcgg cgatgccctc
     2821 ggcgacatcg agcaccgagg acgccgcgag gttctcgtcg gtcatctcgt cggagagctc
     2881 gtgctgccag ttcacctcga tcacgtggat cgcgaccagc tcggcggagt gcagcttggc
     2941 cagctggcac cccaggttga ccaccagctg gtcggtcgga ccgccgttta ggcccaggac
     3001 agccttacga atcgggtttg gattggcgcc ctctggcatg ccgcggaagc ctatcagccg
     3061 cgcgggggcg cggccgctcg ctcatcttcc tcgcgccggt acggcacgtt gacgacgacc
     3121 gtgtgcggcc ggcccaacag cgcgctgcgc agccggttcg cggactggtt gtagaggatg
     3181 cgctcccacc agtggcgcgc cacgtattcg gggatcacga cgaaggtgat cggatcctcc
     3241 ttgtcctccg gccacgtctt gtcgagcacg tcgaggtagg ccagcatcgg tccgaccagc
     3301 gcgcgatagg gcgactcgac gatcaccaag ggcacctggg gcaactggtg ctcccagcgc
     3361 tcgcgcatcc gcgcagcctc ctccggctcg tcggagatca ggacggcccg gatgtcgggc
     3421 gcgatcgagc gcgccacgtt gaccgcctgc acgactgccc ggttgatgcc cgggaccggc
     3481 acgatgacgc gctcctcgcg ccgcggcggc gggatgaccg cctcctcgct catcgcgagc
     3541 tgattggccg acgccgtgta ctggcgatga atgaagacca tgatcaggat gagggccggg
     3601 atgatgatcg tgacgagcag tgaggttggc gccttggcca ccagcacgac gaagaagacg
     3661 acgccggtga gtgcagcgcc aaaggcgttg atgctcatct tcttgcgcca gcccgccgag
     3721 cgctcggcta gccagtgccg gaccaggccc agctggccaa tggtgaacgc catgaacacg
     3781 cccaccgcgt agagcggaat gagcgcggtg gtgacgccgc cgaacgcgac gatcagcgtg
     3841 attgcggcga cgctcaggat cacgatgccg ctggtgaagg cgaggcggtc gccgcggaac
     3901 gagaagacgc gcggcatgaa accgtcgcgc gccaggatgg cggccaggcg cgggaaggcg
     3961 ttgaaagacg tgttcgcggc caggaagagg atcagcgccg tgaacgcctg gtagaggtag
     4021 aacag
//
LOCUS       DATBRX010000236         2835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3123653, whole genome shotgun sequence.
ACCESSION   DATBRX010000236 DATBRX010000000
VERSION     DATBRX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123653"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VIF08_08945"
     CDS             complement(<1..334)
                     /locus_tag="VIF08_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in the transport or adenine; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEY8136151.1"
                     /translation="MEAIASYFKFAERGTTLGTEVKAGLATFMVMVYIVAVNPGILAA
                     AGIDPVAAAAATALVAGVMTILMGFFSNYPIALAAGLGINGIVAFGLVLTLGLTPAGA
                     MGVIVWEGL"
     gene            complement(418..882)
                     /locus_tag="VIF08_08950"
     CDS             complement(418..882)
                     /locus_tag="VIF08_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136152.1"
                     /translation="MSLEPPAKFFVNSGLVSSPTDIYNLLRPLLKQTVSYRYFRGAGL
                     VSIGTGWVERIVVDRPEVSTFFTPLSICLNIDSFEHLEFETRPDQLIVYTLVQGDERI
                     VVEFAPVGRTAADEPEQQRFAFPATSDYVQMELSTLESGDGSDAAGEGSEGS"
     gene            1003..1797
                     /locus_tag="VIF08_08955"
     CDS             1003..1797
                     /locus_tag="VIF08_08955"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY8136153.1"
                     /translation="MIVALDVGNTKIALGVVRSGDVTSSRRAPTRGNRNAEHLAATLD
                     ALLAEDEAALGDVDTILLASVVPGVTTALSALTEERGIRLIVADHTTIPIAVRVDNPG
                     AVGNDRLINAFAAGFLYGMPVIVIDLGTATTLDVVAPDGAFLGGAIAPGLGLGLDALV
                     EHSAQLPEVALEVPEHAIGTDTVSAMLSGAVLGYFGLVKELVRRIRGELGAAKPKVVL
                     TGGLSALPWANSIPSVDIIDPLLTLRGLALLEREVAARPKQPKVRA"
     gene            1808..>2835
                     /gene="coaBC"
                     /locus_tag="VIF08_08960"
     CDS             1808..>2835
                     /gene="coaBC"
                     /locus_tag="VIF08_08960"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY8136154.1"
                     /translation="MSNLEGRLILLGVTGSIAAYKSAELCRSLTTAGADVQTLLSRSA
                     SAFIGALTLETLTRRRPMTDPLELLPDQRIGHIVAADSADAVLVAPATARWLGAMAAG
                     LADDVITATCLATAAPVVVAPAMDGEMWNHPATRRNVETLRGFGYTIVEPEVGPLASG
                     QRGVGRLAETQTILAALEKVLDGKPIRQPDEAQRPPVVMPLPGNDLGGWHFVVTAGGT
                     AEAIDPVRYIGNRSTGKMGVAIAQAALDRGAVVTLIAGMTSAPIPSGVNLVRAPSAAE
                     MRDAVLAALPGADALVMAAAVADFRPRTQSTTKIARAGSGLTLELEPTQDILAEAVTT
                     ARAKAGRQP"
BASE COUNT          463 a         1000 c          942 g          430 t
ORIGIN      
        1 tgagcccttc ccagacgatg acgcccatcg cgcccgcagg cgttaggccc agtgtgagga
       61 cgagaccaaa ggcaacgatt ccattgatgc ccagtccggc cgcaagcgcg atcggatagt
      121 tgctgaagaa gcccatcagg attgtcatga cgccggccac aagggccgtc gctgccgcgg
      181 cggcgactgg gtcaatgccc gcggctgcca gaatccctgg gttcacggcc acgatgtaga
      241 ccatgaccat gaacgtggct aggccggcct tgacctcggt ccccaaggtc gtgccgcgct
      301 cggcaaactt gaaataggaa gcgatcgctt ccaaggtcgc actcctccct ctattgcgga
      361 cccgcgctga cgcgcgcgtc ccactccctc gtggctagca gccagcagcg cggccgctca
      421 gctcccttct gacccctctc ccgcagcatc ggagccatcg ccgctctcga gcgtgctgag
      481 ctccatctgg acgtagtccg atgtggcagg aaaggcgaag cgttgctgct cgggctcgtc
      541 ggcggcagtc cggccgacgg gcgcgaactc gacgacgatc cgttcgtcgc cctggaccag
      601 cgtgtagacg atcagctggt cggggcgcgt ctcgaactcg aggtgctcga acgaatcgat
      661 gttcaggcag atagacagtg gagtgaagaa ggtggaaacc tccggtcggt cgacgacgat
      721 gcgctcgacc cagccggtgc cgatggagac aaggccggca ccgcggaagt agcggtacga
      781 cacggtctgc ttgagaagcg gacgcagcag gttgtagatg tcggtggggc tcgacacaag
      841 gccgctattt acgaagaatt tcgcgggcgg ctcgagcgac acgctcctcc tcacctgttg
      901 gtcagtccgg agcctctccc tgcgcccgga ccggccgatg gtagcgcgtc cggaacacgg
      961 cgcaacgccg ttttcgcacc tatgccgcgt atccttgccc gcgtgatcgt cgcgctggac
     1021 gtgggcaata ccaagatcgc gctgggcgtc gttcggagcg gggacgtcac gagctcgcgc
     1081 cgcgccccga cgcgcggcaa caggaacgcc gaacatctgg cggccacgct cgatgcgctt
     1141 ctcgccgagg atgaggcggc gctgggcgac gttgacacga tcctgctggc aagcgtcgtc
     1201 ccgggggtca cgaccgcgct ctctgcgctg accgaggaac gaggcatccg gctcatcgtc
     1261 gccgaccaca ccaccatccc gatcgcggtc cgcgtcgaca atcccggcgc cgttggcaac
     1321 gatcgactga tcaacgcctt cgcggcgggg ttcctgtacg gaatgccggt gatcgtcatc
     1381 gacctgggca cagcgacgac gctggacgtc gtggcgcccg acggcgcgtt cctgggcggc
     1441 gccatcgcgc ccggactggg attgggcctc gatgccctcg tcgaacactc cgcccagcta
     1501 cccgaagtcg ccctcgaggt cccggagcac gccatcggaa ccgacacggt gagcgccatg
     1561 ctcagcggcg ccgttctggg ctacttcggt ctcgtgaagg agctggtgcg ccggatccgc
     1621 ggcgagctcg gcgcggccaa gcccaaggtc gtgctgaccg gcgggttgtc ggcactgccg
     1681 tgggccaact cgatcccgtc agtcgacatc atcgatccgc tcctcactct gcgcggcctg
     1741 gcgctgctcg agcgcgaggt ggcggccagg cccaagcagc caaaggtgcg cgcatgagga
     1801 atcacgcatg agcaacctgg agggccgcct gatcctgctg ggcgtcaccg gctcgatcgc
     1861 ggcctacaag agcgccgagc tgtgtcgctc gctgaccacg gccggggcgg acgtacagac
     1921 tcttctcagc cgctcagcgt cggcgtttat cggcgcgctc accctcgaga cgctcacccg
     1981 ccgccggccc atgaccgacc cgctcgagct gctgcccgac cagcgcatcg gccacatagt
     2041 cgccgccgac tcggccgacg cggtccttgt tgcgccggcg acggcgcgat ggctgggtgc
     2101 catggcggcc ggtctggccg acgacgtcat caccgcgacc tgcctcgcga cggcggcgcc
     2161 ggtggttgtc gcgcccgcga tggacggcga gatgtggaac caccctgcga cgcggcgcaa
     2221 cgtcgagacg ctacgcggct ttggctacac gatcgtggag ccagaggttg ggccgctcgc
     2281 ctccgggcag cgtggcgtgg gccggctggc cgagacgcag accatcctcg cggcgctcga
     2341 gaaggtcctc gacggcaagc ccatccgcca gcctgacgag gcgcaacgcc cgccagtcgt
     2401 catgcccctc cccggcaatg acctgggcgg ctggcacttc gtcgtcacgg ccggcggaac
     2461 ggcagaggcg atcgacccgg tgcgctacat cggcaaccgc tcgacgggca agatgggcgt
     2521 ggcgatcgcc caggccgcgc tcgaccgcgg cgccgtggtg acgctgattg ccggcatgac
     2581 ctccgcgccc atcccgagcg gcgtcaacct cgtgcgcgcg ccatcagccg ccgaaatgcg
     2641 cgacgcggtc ttggccgctc tgccgggtgc cgacgcgctt gtcatggccg ccgccgtggc
     2701 cgacttccgg ccgcgcaccc aatccaccac caagatcgcc cgcgctggct ccggactgac
     2761 gctcgaactg gagccaacgc aggacatcct ggccgaggcc gtaacgacgg cacgcgccaa
     2821 ggccggccgc caacc
//
LOCUS       DATBRX010000237         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2341107, whole genome shotgun sequence.
ACCESSION   DATBRX010000237 DATBRX010000000
VERSION     DATBRX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341107"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VIF08_08965"
     CDS             complement(<1..285)
                     /locus_tag="VIF08_08965"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8136155.1"
                     /translation="MDDQRVGRVVRALRRRLGWTQSDVAARAACAQATVSETERGNLP
                     SVPILRRVLAALDASMVIDLRWRAGALERLLDEDHARLVGYLVTLLRALGW"
     gene            356..1591
                     /locus_tag="VIF08_08970"
     CDS             356..1591
                     /locus_tag="VIF08_08970"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8136156.1"
                     /translation="MRKVDVVVVGAGQAGLATSYHLTQRGLEHIVLERGDVPGESWRT
                     RRWDTFMLNGPNFSFDLPGYPYDGSDPDAFMARDEIVARFGAYATRIAVPVETRVDVL
                     RLDRTADGRYRATTTSDEIESRAVVVATGAYQKRHRPPGAIDPSIVQMHTDQYRNPAD
                     LPPGGVLVVGAGQSGCQVVEDLLAAGRPTWLSAGSCGWIPRRYRGRDNVLWRQQMGVF
                     NETVEQLGHALRMACPPMQTGVGWDRDINLRTLHAQGATLLGRFRTADAFTATFADDL
                     HQSAARSDEAAVRFRAQVDDFITTNQIDAPTEPPIQPSTGLPKPPTTLDLRQAGISTV
                     IWASGFRLDFESWIDLPLNTVDGYPEQTQGVSRHPGLYFMGLQLMHTRKSGLIFGVGE
                     DAAHVVSVLGGYLGARTQA"
     gene            complement(1616..1897)
                     /locus_tag="VIF08_08975"
     CDS             complement(1616..1897)
                     /locus_tag="VIF08_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136157.1"
                     /translation="MSSYRAAAMANDPREAISALIQLSVLFEADCPVGHSPWGQTVTS
                     CLGDRSTSTTFILRKTGPGTFAGTCHDDILEETATGTLDWSGQVEQISP"
     gene            complement(1894..3489)
                     /locus_tag="VIF08_08980"
     CDS             complement(1894..3489)
                     /locus_tag="VIF08_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136158.1"
                     /translation="MTEAQQVSWPWWMAPARLIGRVRPGLAAAAQSDAIALERLPRLL
                     GTVAVPLTVIAIAAGFAALHAVSGPRTDPPVHLEWLNLSMYNIYTEVPLFIFTACIIG
                     ALSPAAAVLFVLSFGAFDLIAAATHPEELRFWALRPFPIAGRLIAYWLLWLLAVEIPV
                     MGRMMAVSVRGLAGSRIAVAGVSGLLVGTFTWLWTLAAPVLLRPVYLWSDIGGPYAEL
                     GLQLEKGGLVFALIAGVAAGAVAMLVGPAGIMRIRQWPAAKEVGAPTLRGRLIAVVRR
                     LVAAGLLTIGLGGLITTGLDAVFLFVAFAGAGPLAQWLGARIPIAGLVQRIPAPALIA
                     LAIVLVFGVALLTVGVPALASVSEFFPLIAITGVGLFIVELILAPGPARERPPRSIAH
                     AGAIGVGVGLVVVALDFVAPQPALADNYISLKDIYATVTAIAVTVAALVFMVVAATGK
                     WVDIVRERARDVAAQAPYRSDPPGPPPPPPPPEEAPDPFESFGIGPARRLGGSPAPKS
                     KGAPPKGKGTGGPGPQRGKGPEQ"
     gene            complement(3505..>3707)
                     /locus_tag="VIF08_08985"
     CDS             complement(3505..>3707)
                     /locus_tag="VIF08_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136159.1"
                     /translation="SRRRGIVTGVGWALLLETAQLVLYPGWLDIRFLDEFRQISFLSH
                     VVFGLTLGLGIPAGLRWARNRG"
BASE COUNT          670 a         1305 c         1203 g          529 t
ORIGIN      
        1 ccagccaagg gcgcgcagga gcgtcaccag gtagccaaca agccgggcat ggtcttcgtc
       61 gagcagccgt tccaacgcgc ccgcccgcca ccggagatcg atcaccatgg aggcatcgag
      121 cgccgcgagg acgcgacgca gaatcggcac cgacgggagg ttgccgcgct cggtttctga
      181 caccgtcgcc tgggcgcagg ccgccctagc ggcaacatcc gactgggtcc agcccaggcg
      241 gcggcgcaac gcccgcacga cgcggccgac gcgctgatcg tccatacccc aagcgtgagc
      301 aacgtgactt tgctggcgct tactccgggg ttgccggcgg tgacagaatc gcccgatgcg
      361 caaggtggat gtcgtcgtgg tcggcgccgg ccaggccggc ctcgccacga gttaccacct
      421 cacgcagcgc ggcctcgagc acatcgtcct ggagcgcggc gatgtgccgg gggagagctg
      481 gcgcacccgc cggtgggaca ctttcatgct gaacggcccc aacttctcgt tcgacctccc
      541 gggttacccc tacgacggct ccgaccccga cgctttcatg gcgcgcgacg agatcgtggc
      601 gcgcttcggc gcctacgcca ccaggattgc ggtacccgtc gaaacccggg tagacgtcct
      661 ccgcctggac cggaccgcgg atggtcgtta ccgcgcgacc acgactagcg acgagatcga
      721 gtcgcgcgcc gtggtcgtcg cgaccggggc gtaccagaag cgccaccgcc cgccgggagc
      781 tatcgacccg agcatcgtcc agatgcacac cgaccaatac cgcaacccgg ccgacctgcc
      841 gccaggcgga gtgctcgtcg tcggtgccgg ccagtccggt tgccaggtcg tcgaagacct
      901 gctcgcggcc gggcgtccaa cctggctgtc cgccggttca tgcggctgga tcccgcgccg
      961 gtaccgcggc cgggataacg ttctctggcg ccagcagatg ggcgtcttca acgagaccgt
     1021 tgagcagctc ggtcacgccc tgcgcatggc ctgcccgccg atgcagacgg gcgtcggctg
     1081 ggaccgcgac atcaacctgc gcacgctgca cgcacagggc gcgacgctgc tgggccgctt
     1141 ccgcacggcc gacgctttca cagccacctt cgctgacgac cttcaccaga gcgccgcacg
     1201 atctgacgaa gctgccgtcc ggttccgcgc ccaggtcgac gacttcatca caacgaacca
     1261 gatcgatgca ccgaccgaac cgcccatcca gccttcgacc ggtctgccca agccgccaac
     1321 cacactcgat ctccgacaag ccggcatcag caccgtcatc tgggcgagcg gcttccggct
     1381 cgacttcgag agctggatcg acctgcccct gaacacggtc gatggctacc ccgagcagac
     1441 ccagggcgtc agccggcacc cgggtctgta tttcatgggc ctgcagctga tgcatacccg
     1501 caagtcgggg ctgatcttcg gcgtaggcga ggatgccgcc cacgtcgtgt cggtgttggg
     1561 tggttacctc ggcgctcgga cacaggcctg accggccatc agcgattagg agccgctacg
     1621 ggctgatctg ctcaacctgg ccgctccaat cgagcgtgcc agtcgccgtc tcttccagga
     1681 tgtcgtcgtg gcatgtgccc gcaaaggtgc cagggccggt cttccgcagg atgaacgtcg
     1741 tgctcgtgga ccggtcgccc aggcaagagg taacggtctg gccccagggt gagtggccca
     1801 cgggacagtc cgcctcgaat aggacactga gttggatcag ggctgagatc gcctcgcgcg
     1861 ggtcattggc catcgccgcg gctctataac tgctcattgc tccggcccct tgccgcgctg
     1921 tggcccaggc ccaccggtcc ccttgccctt gggcggtgcg cccttggact ttggcgccgg
     1981 ggagccgccc agcctgcgcg caggtccgat gccgaagctc tcgaacgggt ctggcgcttc
     2041 ctcgggcggc ggcggtggtg gcggcggccc tggcggatcc gatcggtacg gagcctgcgc
     2101 agcaacgtcg cgcgcgcgct cgcgcacgat gtccacccac ttgcccgtcg cggcaaccac
     2161 catgaacacg agagcagcga cggtcacggc aatcgccgta accgtcgcgt agatgtcctt
     2221 caggctgatg tagttgtcag ccagtgcggg ctggggggca acgaagtcga gggccacgac
     2281 cacgagtccg actccgacgc cgatcgcccc ggcgtgggcg atcgaccgcg gtggccgctc
     2341 tcgggcgggg ccaggggcca agatgagctc gacgatgaac agtccaacgc cggttatcgc
     2401 tatgagcggg aagaactcgg agacgcttgc gagcgcaggc accccaaccg tcagcagtgc
     2461 cacgccgaag accaacacga ttgcgagggc gatcagcgca ggcgccggta ttcgctgaac
     2521 gagcccagca atcgggatcc tggcgcccag ccattgtgcc agcggaccag ccccggcaaa
     2581 ggcgacaaag aggaatacgg cgtcgagacc cgtggtgatc aacccaccca ggccgatcgt
     2641 caacagcccg gcagctacca gccggcgcac gacagcgatc agccgcccgc gcagcgtggg
     2701 tgcgccgacc tccttcgcgg ctggccattg ccttatgcgc atgatcccgg ccggaccgac
     2761 cagcatcgcg accgccccag ccgccacccc cgcgatcagg gcaaagacca gcccgccctt
     2821 ttcgagctgc aggcccagct ccgcgtatgg accgccaatg tcggaccaca ggtacacggg
     2881 tcgcagcaga acaggcgccg ccagggtcca gagccaggtg aaggtgccga ccagaaggcc
     2941 gctcacgccg gccacggcta tccggctgcc ggcgagaccg cggaccgaga cagccatcat
     3001 tcgccccatt accgggatct cgacggcaag cagccacagc agccaatacg cgatcagtcg
     3061 ccccgcaatt gggaacgggc gcaacgccca gaaccggagc tcctcagggt gcgtcgccgc
     3121 tgcgatcagg tcaaaggcgc caaatgagag cacgaacagc acggcagcag ccggcgagag
     3181 ggcgccgatg atgcaggctg tgaagatgaa gagcgggacc tcggtgtaga tgttgtacat
     3241 gctcaggttg agccactcga ggtgcactgg cggatctgtc cgcggaccag acacggcatg
     3301 cagagctgcg aaccccgccg cgatggcgat gacggtgagc gggacggcca ccgtgcccaa
     3361 cagccttggt agccgctcca gggcgatggc atcggactgc gccgcagcag ccagcccggg
     3421 ccggacgcgt ccgatcagtc gtgccggggc catccaccac ggccagctca cctgttgagc
     3481 ttcagtcatc agtaaccctt cctttcaacc acgattcctg gcccagcgca gtcccgccgg
     3541 gatgccaagg cccagcgtca atccgaaaac gacgtgcgac agaaacgaga tctggcggaa
     3601 ctcgtcgaga aagcggatgt ccagccagcc cgggtagagg accagctggg ccgtttcgag
     3661 cagcagcgcc cagccgacac cggtgacgat gccgcgccgc cgtgata
//
LOCUS       DATBRX010000238         4483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2678090, whole genome shotgun sequence.
ACCESSION   DATBRX010000238 DATBRX010000000
VERSION     DATBRX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2678090"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1435)
                     /locus_tag="VIF08_08990"
     CDS             complement(158..1435)
                     /locus_tag="VIF08_08990"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8136160.1"
                     /translation="MVTPSLWGHSDFVKLWIGQTISRVGSVVTRTAMPLVALLVLGAG
                     PFEMALVIVAQSLAVLLVGLFAGAWVDRLRRRPLLIASDAARALLLFSIPAAYLVGAL
                     SLWQLYVVVFLESCLGVLFNAAYPAYVPSLIGVDRVVEGNSKLAASSSLAEIGGPGLA
                     GGLVQLIGAPFAILVDAASYVISLVSLGLIRKPEPARPARTESTSIRREIADGLRLVS
                     RHPVLVPLTLRSVIAHVAGSFYGVLYTIYLIQVLHLDPLLLGIVVSAGGVGALIGSFF
                     AQRVINRLGFGPAIIWGAVGASLIGVLTPLAGGPVAIATAMVFLPQLIGDGLQTIESV
                     AELSLIQGVIPDHILGRVNATLEVFSHGVAYPAGALLAAFLAEEVGVRGGIALGWAGM
                     ALSILLLVFSPLPRVKKHSDVAAVERFPPARGS"
     gene            1736..>4483
                     /locus_tag="VIF08_08995"
     CDS             1736..>4483
                     /locus_tag="VIF08_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY8136161.1"
                     /translation="MLSVTDPGRRSLIANTERKPYARIPEVLPIPNLIELQLNSFNWF
                     IDHGLRELLDEISPIQDFTGRVMELRFREYEFETPKYSELECRTRDLTFSKPLYVWVE
                     LLIKETGEIQRQRVYMGDFPFMTDEGTFVINGAERVVVSQLVRSPGVYYTATEDPATG
                     RNLFNAKVIPNRGAWLEFETAARGQLYVKVDRKRKLEATKLLRAVGYENNDEMAALFS
                     KIDTGDTKYIASTLDKDNTNTRGEALIEVYKKLRPGDPPTGDNAEKLVESLFFNFRRY
                     DLGRVGRYKLNKKLNAVADRMGINLPTERTISKEDIAAIVGHLIELNRDQGLADDIDH
                     LGNRRIRANGELIQNAFRVGLLRMERVVRERMSIQEAEKATPNALINIRPVVAAMKEF
                     FGGSQLSQFMDQTNPLAELTSKRRLSALGPGGLSRERAGFDVRDVHHSHYGRICPIET
                     PEGPNIGLIGSLATYGRINEYGFIETPYRKVKRTVSADDKGIESEIADADVTTKTGGV
                     LLKKGKAFTKEAVSELKKQKASAFPIKPRVTEEIVYLAADEEEEHHVAQANAKLDAKG
                     YFVDERIPARFRDSFPEARPNQIEYMDVSPKQVVSVATALIPFLEHDDANRALMGSNM
                     QRQAVPLLEPEAPIVGTGMEAKAALDSGQVLTSKVTGVVTSVTAERIAVEADSGELHE
                     YPLDKFVRSNQGTCINQRPIVMVGQRVQAGEPIADSSSTDKGELALGRNILVAFMSWE
                     GGNYEDAIVISDRLVRDDVFTSIHIEKHEIESRDTKLGPEEITRDIPNVGEESLKDLD
                     EYGVIYIGAEVQPGDILVGKITPKGETELTAEERLLRAIFGEKAREVKDSSLRLPHGE
                     RGKIVEVTEFDREKGAELLPGVNKLIRVSVAAKRKISVGDKMAGRHGNKGVVAKILPQ
                     ED"
BASE COUNT          885 a         1557 c         1432 g          609 t
ORIGIN      
        1 agagccgatg gtccggcgga cgatccctac tcgcccgacc tgtcgcttct ccgggccagc
       61 cgagcgcgca tcctggccat ggcaaacctc atcgtgcccg gccacggccc ggcgttcaaa
      121 ccgagcgact ccacaccccg ctaggtgcgc cgcgtcgtca cgacccgcgc gccggcggga
      181 agcgttccac ggcggccaca tcactgtgct tcttgacccg cggcagcggc gagaacacga
      241 ggagcaggat ggagagcgcc atgcccgccc aacccagggc gatgccgccg cggacgccca
      301 cctcctccgc gaggaacgcc gcgaggaggg ctcctgccgg ataagcaacg ccgtgcgaga
      361 acacctcgag cgtggcattc actcggccca ggatgtggtc ggggatgacg ccctggatca
      421 gcgacagctc agccacgctc tcgatcgtct gcaacccatc gccgatgagc tgcggcagga
      481 agaccatcgc ggtcgcgatc gcgaccggcc cgcctgcgag cggggtgagc acgccgatga
      541 gcgacgcacc taccgcgccc cagatgatcg caggaccaaa gcccagccga ttgatcacgc
      601 gctgcgcgaa gaacgatccg atcagcgccc cgacgccacc ggcagagacc acgatcccca
      661 gcagcagcgg atcaagatgc aggacctgga tgaggtagat cgtgtacagc acgccgtaga
      721 acgaccctgc gacatgggcg atgacggagc ggagagtcag cggcaccagg acggggtggc
      781 ggctcaccag gcgcagcccg tcggcaatct cacggcgaat gctcgtggac tctgtccgtg
      841 ccgggcgtgc gggctcgggc ttgcggatca gccccaacga cacgagcgaa atgacgtacg
      901 aagccgcgtc aacgaggatc gcgaacggcg ctccgatcag ttgcactagc ccacccgcca
      961 gaccgggacc gccgatctcc gcgagcgacg agctggccgc gagcttgctg ttgccctcga
     1021 ccacgcgatc gacgccgatc agcgatggca cgtaggccgg atacgccgcg ttgaatagca
     1081 cgcccaggca gctctcgagg aacaccacga cgtagagctg ccacaggctg agcgcgccga
     1141 ccaggtaggc ggcggggata gagaacagca gcaacgcgcg ggccgcgtcg gatgcgatga
     1201 gcagcggccg gcgacgcagc cgatccaccc aggcgccggc aaacaggccc accagcagca
     1261 cggccaggct ctgggccacg atgaccagcg ccatctcgaa tggccccgcg cccaggacca
     1321 gcaatgccac cagcggcatg gccgtccgcg tcaccacgga gccgacgcgc gagatcgtct
     1381 ggcctatcca gagctttacg aaatcggagt gtccccagag ggatggcgtg accactgacc
     1441 cgagattaat gggccgggcg ttgatcgccg gcaccacggg cgcggccggg agcgtcgaca
     1501 ggagttgaca ggagtcgcaa acttgtccgc gccggagccg cgatggcggc ggagcctcgg
     1561 tacgttgcgg cgtgcccggc gttcgcgtgg ccgttgacag gctgcccttc ggtcgatact
     1621 atgtgcggtc gttgagcgcg gtcccctttg tctctggaac tccccggcgg atgaccatgt
     1681 ccgcctttgg ccgtccctgc ccggagctga acgtccgcct cgctaggagc gcttaatgct
     1741 gtctgtcacc gaccccggcc ggcgtagctt gatcgccaac acggagcgca agccgtacgc
     1801 ccgcatcccc gaagtcctac ccatcccgaa cctgatcgag cttcagctga actcgttcaa
     1861 ctggttcatc gaccatgggc tgcgcgagct gctcgacgag atcagcccca tccaggactt
     1921 caccggtcgc gtgatggagc ttcgcttccg cgagtacgag ttcgagacgc ccaagtacag
     1981 cgagctcgag tgccgcaccc gtgacctgac cttctccaag ccgctctacg tgtgggtcga
     2041 gctgctcatc aaggagaccg gcgagatcca gcgccagcgc gtgtacatgg gcgacttccc
     2101 gttcatgacc gacgagggca cgttcgttat caatggcgcc gagcgcgtcg tcgtgagcca
     2161 gctcgtccgc tcgccaggcg tctactacac cgcgactgag gatccggcga ccggccgcaa
     2221 cctcttcaac gccaaggtca tccccaaccg cggcgcgtgg ctcgagttcg agactgctgc
     2281 ccgcggccag ctgtacgtca aggtcgatcg caagcgcaag ctggaggcga ccaagctgct
     2341 gcgcgcggtc ggctacgaga acaacgacga gatggccgcg ctcttctcga agatcgacac
     2401 gggcgacacc aagtacatcg cctccacgct cgacaaggac aacaccaaca cccgcggtga
     2461 ggcgctcatc gaggtctaca agaagctgcg ccctggtgac ccgcccacgg gcgacaacgc
     2521 cgagaagctg gtcgagagcc tgttcttcaa cttccgccgc tacgacctcg gtcgggtcgg
     2581 ccgctacaag ctcaacaaga agctcaacgc ggtggccgat cggatgggca tcaacctgcc
     2641 caccgagcgc accatcagca aggaagacat cgccgcgata gtcggtcacc tgatcgagct
     2701 caaccgtgac cagggtctgg cggacgacat cgaccacctc ggcaaccggc gcatccgtgc
     2761 caacggtgag ctgatccaga acgccttccg cgtcgggctg ctgcgcatgg agcgcgtggt
     2821 ccgcgagcgc atgtcgatcc aggaggcaga gaaggccacg cccaacgccc tcatcaacat
     2881 ccgcccggtc gtggcggcga tgaaggagtt cttcggcggc agccagctca gccagttcat
     2941 ggaccagacc aacccgctcg ccgagctcac cagcaagcgc cgcctgagcg cgctcgggcc
     3001 gggtggcctc agccgcgagc gcgccggctt cgacgtgcgc gacgtgcacc acagccatta
     3061 cggccggatc tgcccgatcg agaccccgga aggcccgaac atcggcctga tcggatcgct
     3121 ggccacctac ggccggatca acgagtacgg cttcatcgag acgccctacc gcaaggtcaa
     3181 gcgcaccgtc tcggccgacg acaagggcat cgagagcgag atcgccgacg ccgacgtcac
     3241 caccaagacc gggggcgtcc tgctcaagaa gggcaaggcc ttcaccaagg aagcggtcag
     3301 cgagctcaag aagcagaagg cgtccgcctt cccgatcaag ccgcgcgtca ccgaggagat
     3361 cgtctacctc gccgccgatg aggaagagga gcaccacgtc gcgcaggcca acgccaagct
     3421 tgacgccaag ggctacttcg tcgacgagcg catcccggcg cgcttccgcg actcattccc
     3481 ggaggcgcgg cccaaccaga tcgaatacat ggacgtctcg cccaagcagg tcgtgagcgt
     3541 cgccacggcg ctcatcccgt tcctggagca tgacgacgcc aaccgcgccc tcatgggctc
     3601 gaacatgcag cgccaggcgg tgccgctgct cgagccggag gcgcccatcg tgggcaccgg
     3661 catggaggcc aaggcggcgc tcgactccgg ccaggtcctg acctccaagg tcacgggcgt
     3721 ggttacgagc gtgaccgccg agcgcatcgc ggtcgaggct gactcgggcg agctgcacga
     3781 gtacccgctc gacaagttcg tgcgctccaa ccagggcacg tgcatcaacc agcgtccgat
     3841 cgtcatggtc ggccagcgcg tccaggccgg cgagcccatc gccgactcga gctcaaccga
     3901 caagggtgag ctcgccctgg gccgcaacat cctggtcgcg ttcatgtcgt gggagggcgg
     3961 caactacgag gacgcgatcg tcatcagcga ccgcctcgtg cgcgacgacg tcttcaccag
     4021 catccacatc gagaagcacg agatcgagtc gcgcgacacc aagctcggcc cggaggagat
     4081 cacgcgcgac atccccaatg tcggcgagga gagcctcaag gacctcgatg agtacggcgt
     4141 catctacatc ggcgctgaag tccagccggg cgacatcctc gtcggcaaga tcacgcccaa
     4201 gggcgagacc gagctgaccg ctgaagagcg cctcttgagg gcgatcttcg gtgagaaggc
     4261 gcgcgaggtg aaggactcca gcctgcgcct gccccacggg gagcgcggca agatcgtcga
     4321 ggtcaccgag ttcgaccgcg agaagggtgc cgagctgctg cccggcgtca acaagctgat
     4381 ccgcgtcagc gtcgcggcca agcgcaagat cagcgtcggc gacaagatgg ccggccgcca
     4441 cggcaacaag ggcgtcgtgg ccaagatcct gccccaggag gac
//
LOCUS       DATBRX010000239         5050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_666863, whole genome shotgun sequence.
ACCESSION   DATBRX010000239 DATBRX010000000
VERSION     DATBRX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666863"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VIF08_09000"
     CDS             <1..767
                     /locus_tag="VIF08_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136162.1"
                     /translation="VSGVVLTSGEEIGASRVVSGLDPRRTMLGLLDPMDLGPTLGWEA
                     ANLRQHGGTSAVLFALRGLPAFGAMSPGEAAERLSGRILFGQSINVLDRAGDAIKGGR
                     LPETLVLEAVIPTLLDASLVPGNGVSHLMSVLVHGTPYRLRDGDWDAGREALADRVVA
                     QIEAHAPGFAANVVARRVLTPLDLERDYGMTEGHPLHAEPALDQWFAWRPLLGAARYR
                     LPVADGLFLCGSGAHPGGGVTGMPGYLAAREVIGAK"
     gene            818..4363
                     /locus_tag="VIF08_09005"
     CDS             818..4363
                     /locus_tag="VIF08_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8136163.1"
                     /translation="MICASCGSENAPGAKFCNECGATLAAGCPNCGAINKPGARFCNE
                     CGTALAGGAPAATGATGAPVRPATPSAAPPVAERRLVTILFADIVGFTPFAEERDAEE
                     VRETLSRYFDMCSEVIGRYGGTVEKFIGDAVMAVWGAPVAFEDDAERAVRAALELVAA
                     VPALGPGAQARGGVLTGEAAVTLGATNQGMVAGDLVNTAARLQGVAPPGTVLVGEATM
                     RATSGSVIYEEAGEQLLKGKQSPVSAWRAVRVVAERGGRNRSDALEAPFVGRSDELRL
                     LKDLFHATGREKRIRLVSLIGPAGIGKSRLAWEFLKYVDGLVETTYWHDGRSPAYGEG
                     ITFWALGEMIRGRCGLTEGDDEETTRTKVAESVRQWVTDPDEIEWVEKALLTLLGIES
                     GMAAEQLFGAWRTYFERIAQLGTVTLVFEDLHHADSGLLDFIEHMLDWSRGLPIYIIT
                     LSRPDLLERRPDWGAGKRNFTSQYIEPLAEPEMRELLAGLVPGLPDAAVTTIVGRADG
                     IPLYAVETVRSLVADGRLKEQDGVYVPVGDLTTLAVPETLTALISSRLDALEAAERSL
                     IHDAAVLGQSFSAQALSAISGVEPDELARRLNGLMRREILTREGDARSAEVGQYVFVQ
                     ALIREVAYNTLSKKDRKARHLAAARFFEQLGSDELASALAGHYLAAYENAGEGPEQDA
                     LAAQARVALKAAAERAASLGGYDQAVVFLRQAVSVTTQSRDRAEILERASDFAGIASR
                     HSLATELGRQAVAAHAESGDRIGMARAHALLGWAMLNGRLDTEARDLLEVAVAEFADL
                     EDAVVLADLKANLARAYNQFEHAGTEPKALTIIDEALAVAEHGNHPSLLAKSLLTKGG
                     ALGSVGRLREAIALFRASEEIAREHGLTDQVLVALTVRGYFLGEVDNVEAYKCFSDGL
                     ALARRVGHRALEQEFVNNFGYTSFLVGEWDRGLAEFDTVLLRELEPTQRIWNMSNMLI
                     IRASRGDDIADELAELEQLATMVTDPHVLTAPNDTLANLAQAEGRFADAQQHWLKIAD
                     GWASQAPASYYQSARPALWGSDLDALRRHFTAIEATGVHGPVVEVRRTNMRAAIAALE
                     GRSREALALYGEAFAGWGNLKVTWEQALTGLDMATVLDNSEPAVAAAIRFARDTFTRL
                     GAKPYLDRLEMVIARGSAKSAKPNRATEATVAEPA"
     gene            complement(4360..>5050)
                     /gene="surE"
                     /locus_tag="VIF08_09010"
     CDS             complement(4360..>5050)
                     /gene="surE"
                     /locus_tag="VIF08_09010"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY8136164.1"
                     /translation="SAVGHTKTFMRPLRVRERTLADGSRAWSADGSPTDAVSLALLGY
                     FDLGFDLVASGINYGANLGDDITYSGTVSAAMEAVLSSVPAFAISQEYYEHPDFTLAG
                     LIAYRAAVNILENGLAPGELININVPGIAVEECQGVEVTRLGKRVYQDELVERIDPRG
                     TPYYWLGGPPPTGLAEPGTDFDAVVNKRIAITPIQLDLTARRLLKRLNSWSWDIETTA
                     PPPDEAGHNKG"
BASE COUNT          789 a         1696 c         1776 g          789 t
ORIGIN      
        1 gggtaagcgg tgtcgtgctg acctccggag aggagatcgg tgccagcagg gtcgtgtcgg
       61 gtctggaccc gcgccggacg atgctcggtc tgctcgatcc gatggatctt ggccctaccc
      121 tcggctggga ggcggccaac ctgcgccagc acggagggac gtccgcagtg ctcttcgcgc
      181 tgcgcggtct gcccgcgttc ggcgccatgt cgccgggcga agcggcagag cgactgtcgg
      241 gacgcatcct cttcggccaa tcgatcaacg tgctcgatcg tgccggcgac gcgatcaagg
      301 gtggccgcct gcccgagacg ctcgtcctgg aagcggtcat tccgacgctg ctcgatgcgt
      361 cgctggtgcc cggcaatggc gttagccacc tgatgagcgt gctggtgcac ggcacgccgt
      421 accggctgcg cgacggcgac tgggacgcgg gacgcgaagc gctcgccgat cgggtcgtgg
      481 cccaaatcga ggcgcacgcg ccaggcttcg ccgcgaacgt ggtggcgcgg cgggtgctga
      541 cgccgctcga cctggagcgc gattacggca tgacggaggg ccatccgctc cacgccgagc
      601 cggcgctgga ccaatggttc gcctggcggc cgctgctggg cgccgcgcgc taccgattgc
      661 ccgtggccga tggcctcttc ctgtgcggct cgggcgcgca cccgggtggc ggcgtgaccg
      721 gtatgcctgg ctatctggcc gcgcgcgagg tcatcggcgc caagtagtcc gcggccgcac
      781 gtgccgtgcc cgactagcgt ggtcgtaaag tacgcccgtg atctgcgcta gctgcggaag
      841 cgagaacgca ccgggcgcca agttctgtaa tgaatgcggt gcgaccctgg cggccggctg
      901 cccgaattgc ggcgcgatca acaagcccgg ggccaggttc tgcaacgagt gcggcacggc
      961 cctggccggc ggcgctcccg cggccacggg cgcgaccggc gcgcctgtgc ggccagccac
     1021 cccatccgcc gccccaccgg tcgccgagcg gcgcctcgtc acgatcctct ttgccgacat
     1081 cgtcggcttc acgccgttcg ccgaggagcg cgacgcggaa gaagtgcgcg aaacgctgtc
     1141 gcgctacttc gatatgtgca gcgaggtcat cgggcgctac ggcggaaccg tcgagaagtt
     1201 catcggcgac gcggtgatgg cggtgtgggg cgccccggtc gcgttcgagg atgacgccga
     1261 gcgcgcggtt cgtgccgcgc tcgagctggt cgccgccgtg cccgccctgg gtccgggtgc
     1321 ccaggcacgc ggcggagtcc tcaccgggga agcggccgtc accctgggcg caaccaacca
     1381 gggcatggtc gcaggcgacc ttgtcaacac cgctgcccgc ctccagggcg tggccccgcc
     1441 gggcactgtg ctagtcggcg aggcgacgat gcgcgcgacg agcggctcgg tcatctacga
     1501 ggaggcgggc gagcagctcc tcaagggcaa gcagtcaccg gtgtcggcgt ggcgggcggt
     1561 gcgcgtcgtg gccgagcgcg gcgggcgcaa ccggtccgat gcgctggaag cgccgttcgt
     1621 cggccggtcc gatgagctcc ggctgctcaa ggatctgttc cacgcgacgg ggcgcgagaa
     1681 gcgcatccgt ctggtgtcgc tcataggccc cgccggaata ggcaagtcac gcctcgcctg
     1741 ggagtttctg aagtacgtcg acggactggt cgaaacgacc tactggcatg acggccgctc
     1801 accggcctac ggggagggca tcactttctg ggccctgggc gagatgatcc gcggccgctg
     1861 cggcctgacc gaaggtgacg acgaggaaac gacgcgcaca aaggttgccg aaagcgtgcg
     1921 ccagtgggtc accgatcccg atgagatcga gtgggtagag aaggcgctgc tcacgctgct
     1981 cggcatcgag tcgggcatgg ccgcggagca gctcttcggt gcctggcgca cctacttcga
     2041 gcggatcgcg cagctcggca ccgtgacgct tgttttcgag gacctgcatc acgccgactc
     2101 gggcctgctc gacttcatcg aacacatgct cgattggagc cgtgggctgc cgatctacat
     2161 catcaccctg tcccgaccgg acctgctcga gcgccggccg gactggggcg ccggcaagcg
     2221 caacttcacg tcgcaataca tcgagccgct ggccgagccg gagatgcgcg agctcctggc
     2281 gggtctcgtg cccggactgc cagatgcggc cgttacgacc atcgtcggcc gcgcggacgg
     2341 gatcccgctg tacgcggttg agaccgtgcg ctcgctcgtc gccgacggac ggctcaaaga
     2401 gcaggacggc gtatacgttc cggtgggtga cctgacgacc ctcgcagttc cggaaacgct
     2461 cactgcgctc atctcatcgc ggcttgacgc gctcgaggcg gccgagcgat cgctcatcca
     2521 cgatgccgcc gtattgggcc agtcattcag tgcgcaggcg ctcagcgcca tatccggtgt
     2581 cgagccggac gagctcgcgc ggcgtctcaa cggtctgatg cggcgcgaga tcctcacgcg
     2641 cgagggtgac gcgcgttcag ccgaggtcgg ccagtacgtc ttcgtccagg cgctcatccg
     2701 cgaggtcgcc tacaacacac tttcaaagaa agatcgcaag gcgcgtcacc tcgccgccgc
     2761 gcgtttcttc gagcagctcg gcagcgacga gctggccagt gccctcgcgg gccattacct
     2821 cgcggcatat gagaatgccg gcgagggccc ggagcaggac gctctcgccg cccaggcgcg
     2881 cgtcgccctg aaggccgcgg ccgaacgggc ggcgtcgctg ggcggctacg accaggcggt
     2941 cgtcttcctg cggcaggctg tgagcgtcac gacacagtcc cgggatcgcg ccgagattct
     3001 cgagcgggcg agcgacttcg ctggaattgc ctcgagacac tcgttggcga ccgagctcgg
     3061 gcggcaggcg gttgccgccc acgccgaatc gggcgacagg atcggcatgg cgcgcgcgca
     3121 cgcgctcctg ggctgggcga tgctgaatgg tcgcctggat accgaggcgc gcgacttgct
     3181 cgaagtcgcg gtggccgagt tcgctgacct cgaggacgcc gtggtgctgg ccgacctcaa
     3241 ggcgaacctt gcgcgcgcct acaaccagtt cgagcacgcc ggcaccgaac caaaggcgct
     3301 gacgatcatc gacgaggcac tggccgtggc tgagcacggc aatcatccaa gccttctggc
     3361 caaatcgctg ctcaccaagg gtggcgcgct gggatcagtc gggcggttgc gcgaggccat
     3421 cgcgctcttc cgtgcatccg aggagatcgc gcgcgagcac ggcctcaccg accaggtgct
     3481 ggtggccctc acggtgcgtg gctacttcct gggcgaggtc gataacgtcg aggcctacaa
     3541 gtgcttcagc gacggtctcg cactggcacg ccgggtgggc caccgcgctc tcgagcagga
     3601 attcgtcaac aacttcgggt acacctcctt cctggtgggc gagtgggacc gcgggctggc
     3661 cgagttcgac acagtgctgc tgcgcgagct cgagccgacc cagcgcatct ggaacatgag
     3721 caacatgctg atcatccgcg cgtcacgggg cgacgacatt gccgacgagc tggccgagct
     3781 cgagcagctg gccaccatgg tgacggaccc ccacgtgttg acagcgccca acgacaccct
     3841 cgccaatctc gcccaagcgg aaggccgttt cgccgacgcc cagcagcatt ggctgaagat
     3901 cgcggatggc tgggcgagcc aggcaccggc gtcgtattac cagtccgccc gaccggcgct
     3961 ctggggcagt gaccttgatg ccctgcgtcg ccatttcacg gccatcgagg cgacgggtgt
     4021 tcacgggccc gtcgtggagg tccgccgcac caatatgcgc gcggcgattg ccgccctgga
     4081 aggccgctcg cgcgaggcct tggcgctcta tggcgaggcc ttcgccggct ggggcaacct
     4141 caaggtcacc tgggagcagg cgctcaccgg actcgacatg gcgacagtgc tggataattc
     4201 cgaacccgct gtcgccgcgg cgatcaggtt tgcgcgcgac accttcacgc gcctgggcgc
     4261 caagccctac ctcgatcggc tggagatggt cattgcccgc ggctcggcca agtcggccaa
     4321 gcccaaccga gccaccgaag ctactgtcgc cgagccggcc tagcccttgt tgtggcccgc
     4381 ctcgtcaggc ggcggcgcgg tggtctcgat gtcccacgac caggagttca agcgcttgag
     4441 cagccgccgc gcggtgaggt cgagctgaat gggcgtgatc gcgatgcgct tgttgacgac
     4501 cgcgtcaaag tcggtgcccg gctccgcgag ccctgtcggc ggcgggccgc cgagccagta
     4561 gtagggcgtc ccgcgcgggt cgatgcgctc aaccagctcg tcctggtaga cgcgcttgcc
     4621 gagccgcgtg acctccacgc cctggcactc ctcgacggcg atgccgggca cattgatatt
     4681 gatcagctca cccggagcga ggccgttctc gaggatgttc accgcggcgc ggtaggcgat
     4741 gagaccggcg agggtgaagt cgggatgctc gtagtactcc tgcgagatcg cgaaagccgg
     4801 caccgacgaa aggacggcct ccatggccgc gctgaccgtc cccgaatacg tgatgtcgtc
     4861 gccgagattg gcgccgtagt tgatgcccga cgccacgagg tcaaagccga gatcgaagta
     4921 gccgagcagc gcgaggctga ccgcgtcggt gggtgagcca tcggccgacc aggcgcgcga
     4981 gccgtcggcc agcgtgcgtt cgcgcacccg caacgggcgc atgaaggtct tggtgtggcc
     5041 caccgccgac
//
LOCUS       DATBRX010000240         7268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_2569001, whole genome shotgun sequence.
ACCESSION   DATBRX010000240 DATBRX010000000
VERSION     DATBRX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569001"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VIF08_09015"
     CDS             complement(<1..671)
                     /locus_tag="VIF08_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEY8136165.1"
                     /translation="MNDAELAAREVEIPNTGVTVHDVTFDEAVELIVRWAREGSGGYV
                     STPNVDHLVRTHREPDFRALVRGARLRLPDGMGLIYGMRLRGRRLKGAVHGRLLPEAI
                     VRTAGDSVAIAMIGGRDDAPARATERLTELGGKVVAAVSPPMGFEIGGAADEEATRGL
                     VAAEPRVVIVGFGAPKQERWMARHAAEFPHTVMLGLGQTIDVLGGRVPSAPAWMTRVG
                     LEWAFR"
     gene            complement(668..1564)
                     /locus_tag="VIF08_09020"
     CDS             complement(668..1564)
                     /locus_tag="VIF08_09020"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY8136166.1"
                     /translation="MKRPDFFIVGAFKAGTTALYEYLRAHPQIFMSVPKEPMYFGADL
                     TPRYRRMTGPEYLALFKDAKADQRAGEASPWYLYSSSAATEIKAFEPAARAIILLRNP
                     VDVMYSQHSQLVFNQREDLVDFGLALEAEPDRMAGRRIPPDAIRPEALFYRKSVRFPE
                     QVRRYLEVFGRDAVHFVVFDDLVADAARVYRSTLEFLGVDPSHPLDASVYNPNRSARS
                     GRVQRAIFAPPPALRGLFGRLRRIPLLHRVRDAVVNANSRQAQRARMDPALRAALTAE
                     FEPQVAELGEIIGRDLSAWSRA"
     gene            complement(1561..2517)
                     /locus_tag="VIF08_09025"
     CDS             complement(1561..2517)
                     /locus_tag="VIF08_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEY8136167.1"
                     /translation="MPRPDFLIVGAFKSGSTALYEGLRRHPQIFMPFHKEPLYFGDDL
                     TRRYGRYTEAEYLHLFDTAKPGQKAGEASTWYLYSVSAAREIHDFNPEMRIMVVLRNP
                     VDVMYAQHSQLIYNVIEDIPDFAAALAAEPDRRAGRRIPPGPINIENLFYRHSVRFAE
                     QLERYFDVFGRDRIHVMLWDDLRRDGASVVRGALDFLGVDSSLAAAPPTANENKRVRS
                     PMLQRLIFAPRLLLPLAPFLRRFPLVRAIRTRMLAMNSEAQPRAPMDPALRRQLLDEF
                     APEIERLGRLVGRDLSAWLERDGSAAANQAPAATTPASTTTA"
     gene            complement(2629..4329)
                     /locus_tag="VIF08_09030"
     CDS             complement(2629..4329)
                     /locus_tag="VIF08_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEY8136168.1"
                     /translation="MAGGERDKRDRDGRAPRRDEWRPTDHASATGALQGRVARGLTWT
                     LLDTWGSQLLGLFIFILLARLLTEIDFGLVTLAAVFVAFAQLLVDQGLGDAVVQRPQL
                     TRRQLDTAFWASVVTGLLITGASVVLAIPISILLGDRRLEPIIQVLSIIFVLTALSSI
                     QMGLLRREMAFRSLALRRLVAVALGGVVGVGMALAGFGAWALVGQQIASALVSVVTLW
                     AVTPWRPSFQFSRQDFGELFGYGINVVGGDMLNFLSRNVDRLLIGVFLGVIPVALYGV
                     GYRILDTSQTLLVNAARKLVFPTFSRLQHDPERIRRAYSRINRASSALTLPGYVGLAM
                     VAQEATVAVFGARWTESGPVAAVLFLAGPVLTVQAFSGAVFNAVGHPEVTLRFRLVTT
                     ITNVVGFLVAVLVFRDIVAVAAAFVLRGYLLLPINLYWMRKYAGIPIREHLLELKGVA
                     AATAVMAIAVLLLKIALVSHVHAGVLLLVEVLVGAAVFAVALLVFERALVAEIVGVAG
                     QALPGVGRIARLLHLPAAPPGGKKARPADDSVEAEIEAGAELDPTGLADEDSEKNPLP
                     "
     gene            4374..5369
                     /locus_tag="VIF08_09035"
     CDS             4374..5369
                     /locus_tag="VIF08_09035"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY8136169.1"
                     /translation="MSRTPNLFIVGAPKSGTTSLYEYLKGHPQVFMSAVKEPCYFATD
                     LAFDRSGNFLVYERDAALYQELFADAGDAQLVGEGSTRYLYSHSAPALIHRASPDARI
                     IAMLRNPVDMIQSLHAHKLAGGTEDLASLEEALDAEADRAAGRRLPEFSNPKLATYRD
                     RARYGEQLPRWFDEFGRDRVRVIIFEDMVRDTPGEFRALLEFLGVDPTYAPESFMAHN
                     AAHGTRSPLVRRLVRGRVAQWLAWGVLPRIIGATRTRSMAQRFGHSRMNRRTVPRGKL
                     TPELRRRLEDDFTPDVARLSSLLGRDMSQLWFGRPISGSAGAVPEPAAAHAVSAD"
     gene            5366..5896
                     /gene="cysC"
                     /locus_tag="VIF08_09040"
     CDS             5366..5896
                     /gene="cysC"
                     /locus_tag="VIF08_09040"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY8136170.1"
                     /translation="MSHGACLWFTGLSGSGKTTTAEALIPLLERRGRIVTMLDGDVVR
                     THLSRGLGFSREDRDENVRRVGFVAAEIARHGGLVVCALVSPYRATRAEVRAMVDAAA
                     GPGAFIEIHVDTPLEEVMARDVKGLYAAGTQQMTGLSDPYEPPLTPELRLETVGRSVG
                     QNAAQVLDLLEERGLL"
     gene            complement(5900..>7268)
                     /locus_tag="VIF08_09045"
     CDS             complement(5900..>7268)
                     /locus_tag="VIF08_09045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8136171.1"
                     /translation="AWTALIFHEQGIDLLHPKIPVFGPPWDVPFEFPLFQALGALVMN
                     AGVEPDVAMRVTGMACFLATMLVLSRLVARIAGEAAALVAMLAFAVCALALLWARTSM
                     IEYFVVAASLTTVYAALRWHESRHWAWWALALGAGVIAMMVKPTTAVMYLPAILALAW
                     TGFRAGRERPLGKPLLYAGALLALIAVPLLFGGLWTVYADKVKAASEFTANQTSAALV
                     TWNFGSIDQRLNPDTWGLILGRVNELVLGGQTYFVPFAIGFAWIPLGFLASLWLKRRL
                     FTIAWLAGAFIGPLVFVNLYYIHDYYLIALTPMAAAVVGIGLVWTMRKWKWILPPLLG
                     AFLVVCWIGNLKTSEHYWVIQFYDTHLGEHHLEAAEYIDQRSAPGDMIVMVGRGWNPA
                     TFYYAQRWGLMLEGNEQDQLERALGELLPQLRADGYTRLFDCPPGKQCIAAYDLTTDP
                     PRLLP"
BASE COUNT         1204 a         2476 c         2547 g         1041 t
ORIGIN      
        1 cggaatgccc actccaagcc gacccgggtc atccacgccg gcgcgctcgg gacgcggccg
       61 ccgagcacgt cgatcgtctg gcccaggccg agcatcacgg tgtgcggaaa ctcggccgcg
      121 tggcgcgcca tccagcgctc ctgcttgggc gcgccgaacc cgacgatcac tacgcgcggc
      181 tcggccgcga cgagcccacg tgttgcctcc tcgtcagcgg caccgccgat ctcgaagccc
      241 atcggcgggc tgaccgcggc gacgaccttg ccgcccaact cggtcagccg ctcggtggcg
      301 cgcgccggcg cgtcatcgcg gccgccgatc atcgcgatcg cgaccgagtc gcccgcggtg
      361 cgcacgatcg cctccggcag caagcgccca tggaccgcgc ccttcagtcg gcggccgcgc
      421 aagcgcatgc cgtagatcag gcccatgccg tcgggcagcc ggaggcgcgc accgcgcacg
      481 agcgcccgga agtcaggctc gcggtgcgtc cgcacgagat ggtcgacatt gggcgtcgag
      541 acgtagccgc cgctgccctc gcgcgcccag cgcacgatga gctccacagc ctcgtcgaag
      601 gtcacgtcgt gaaccgtcac gccggtattg ggtatctcga cctcgcgcgc ggccagctcg
      661 gcgtcgttca tgccctgctc cacgcggaga gatcgcgccc gatgatctcg cccagctcgg
      721 cgacctgcgg ctcgaactcg gcggtcagcg cggcgcgcag cgccggatcc atgcgcgcgc
      781 gctgcgcctg ccggctgttg gcgttgacga ccgcgtcgcg gaccctgtgc agcagcggga
      841 tccggcgcaa gcggccgaac aggccgcgca gcgccggggg cggggcgaag atggcccgct
      901 gaacacgccc ggagcgcgcc gagcggttgg ggttgtagac cgacgcgtcg agcgggtggc
      961 tgggatcgac acccaggaac tcaagagtgg atcggtagac acgcgctgca tcggcgacca
     1021 ggtcgtcgaa caccacgaaa tgcaccgcgt cgcgcccgaa cacctcgagg tatctgcgca
     1081 cctgctcggg gaagcggacc gacttgcggt agaagagcgc ctcaggccgg atggcgtcgg
     1141 gcgggatgcg ccggccggcc atccgatccg gctcggcttc aagcgccagg ccgaaatcga
     1201 ccaggtcctc gcgctggttg aagacgagct ggctgtgctg cgaatacatc acgtcgaccg
     1261 ggttgcgcag caagatgatc gctcgcgccg cgggttcgaa agccttgatc tccgtcgctg
     1321 ccgatgacga atagaggtac cagggcgacg cctcccccgc gcgctggtcg gccttggcat
     1381 ccttgaacag cgccaggtat tcgggccctg tcatgcgccg gtaacgtggc gtgaggtcag
     1441 cgccaaagta cataggctcc ttgggcacgc tcatgaagat ctgtgggtga gcgcgcaggt
     1501 actcgtacag cgccgtggtg cccgccttga acgccccgac gatgaagaag tcgggccgtt
     1561 tcacgcggtt gtcgttgacg cgggcgttgt tgcggcaggc gcctggttcg cggcggccga
     1621 tccatcgcgc tcgagccacg ccgagaggtc gcgcccgacg agtcggccca ggcgctctat
     1681 ctccggcgcg aactcgtcga gcagctgccg tcgcaacgcg ggatccatgg gcgcgcgcgg
     1741 ctgcgcttcc gagttcatgg cgagcatccg cgtgcggatg gcccgtacca gcgggaagcg
     1801 gcgcaggaac ggcgcgagcg gcaggagcag ccggggcgcg aatatcaggc gttgcagcat
     1861 tggcgagcgg acacgtttgt tctcgttggc agtgggtggt gccgcggcca gagacgagtc
     1921 gacgcccagg aaatcgagcg cgccgcggac cacggacgcg ccgtcacggc gcaggtcgtc
     1981 ccacagcatc acgtggatcc ggtcgcggcc gaagacatcg aagtagcgct cgagctgctc
     2041 ggcgaagcgc acgctgtggc gatagaagag gttctcgatg tttatcgggc caggcgggat
     2101 gcgccggccg gcacgacggt cgggctcggc cgcgagcgcc gccgcgaagt cgggaatgtc
     2161 ctcgatgacg ttgtagatga gctggctgtg ctgggcgtac atgacgtcga ccgggttgcg
     2221 cagcacgacc atgatccgca tctccggatt gaagtcgtgg atctcgcgcg ccgcggacac
     2281 cgagtacagg taccaggtgc tcgcctcacc cgccttctga ccgggcttgg ccgtgtcgaa
     2341 caggtgcagg tactcggcct ccgtgtagcg accgtagcgg cgcgtcaggt catcgccgaa
     2401 atacagcggc tccttgtgga acggcatgaa gatctggggg tggcggcgca gtccctcgta
     2461 gagcgccgtg ctgccgctct tgaacgcgcc gacgatcaag aaatcaggtc ggggcatgcg
     2521 ctgcgaagga tagcgggggc tcaaccgacc cttcccttgc agcgacgcct gcgggctgct
     2581 gacgcgcgtc ctcagctggc cgccggaatg cggctgccag gttcgtcttt acggcagtgg
     2641 gttcttctcc gagtcctcgt cggcgaggcc ggtcgggtcg agctcggcgc ccgcttcgat
     2701 ctcggcctcc acgctgtcat cggccggccg ggctttcttc ccgcctggag gggccgcggg
     2761 caggtggagc agccgggcaa tacgcccgac gcccggcagg gcctgacccg cgacgcccac
     2821 gatctccgcc accagggcac gctcgaagac cagcagcgcc acggcgaaca ccgccgcccc
     2881 gaccagcact tcgacgagca gcaggactcc ggcgtgaacg tggctaacca gggcgatctt
     2941 gaggagcagc acggcgatcg ccatgaccgc ggtcgcggcg gctacgccct tcagctccaa
     3001 caggtgctcg cgaatcggga tgccggcgta cttgcgcatc cagtacaggt ttatcggcag
     3061 gagcaggtag ccgcgcagaa cgaaggccgc cgcgacggcg acgatgtcgc ggaagacgag
     3121 cactgcgacc aggaagccga cgacgttggt gatcgtcgtc acgaggcgga agcgcagggt
     3181 gacctcgggg tggcccaccg cgttgaacac agcgccgctg aaggcctgga cggtcagcac
     3241 cgggccagcc aggaagagca cggcggcgac cggtccgctc tcggtccagc gggcgccgaa
     3301 gaccgcgacg gtggcttcct gcgcgaccat ggccaggccc acgtagccgg gcagcgtcag
     3361 tgcgctgctg gcgcggttga tgcgcgagta cgcacgcctg atgcgctcgg ggtcgtgctg
     3421 tagccgcgag aacgtcggga agaccagctt gcgcgcggcg ttcacgagca aggtctggct
     3481 ggtgtcgagg atgcggtagc cgacgccgta cagagccacc gggatcactc ccaggaacac
     3541 gccgatcagc agccggtcga cgttacggct caggaagttg agcatgtcgc cgccgacgac
     3601 gttgatgcca tagccgaaga gctcgccgaa gtcctggcgc gagaactgga agctgggccg
     3661 ccacggggtg accgcccaca acgtgaccac gctcacgagg gcgctggcga tctgctggcc
     3721 gacgagcgcc cacgccccga agccggcgag ggccatgccc acacctacca cgccgcccag
     3781 cgccaccgcg accaggcgcc gcagcgcgag gctgcggaag gccatctcgc gccgcagcag
     3841 gcccatctgg atgctgctca gggcggtcag cacgaagatg atcgacagca cctggatgat
     3901 cggctccagg cgccgatcgc caagcaggat cgagatcggg atggccaaga ccacgctcgc
     3961 gcccgtgatg agcaggccgg tcaccactga tgcccagaac gcggtgtcga gctggcggcg
     4021 ggtgagctgc gggcgttgga cgaccgcgtc gcccaggccc tggtcgacaa gcagctgggc
     4081 aaacgcgacg aaaaccgccg ccagcgtcac caggccaaag tcgatctctg tcagcagccg
     4141 cgcgagcagg atgaagatga agaggccgag cagctggctg ccccacgtgt cgagcagcgt
     4201 ccaggtcagc ccgcgcgcga cgcgcccctg cagcgcgccg gtcgccgacg cgtggtcagt
     4261 cgggcgccac tcgtcgcgcc gcggcgcgcg cccgtccctg tcgcgtttgt ccctctcgcc
     4321 gccggccatt cgcgcccgag tgtagggcgc aaccgactat catcccgtgc gccgtgtcac
     4381 gcaccccgaa cctgttcatc gtcggcgcgc ccaagagcgg cacgacatcc ctgtacgagt
     4441 acctcaaggg ccacccgcag gtcttcatgt cggcggtcaa ggagccgtgc tatttcgcga
     4501 ccgacctggc gttcgaccgc tccggcaact tccttgtcta tgagcgcgac gcggcgctct
     4561 accaggagct gttcgccgac gcgggcgatg cgcaattggt cggcgaggga tcgacgcgct
     4621 acctgtactc gcactctgcg ccggcgctga tccaccgggc ctcgccggac gcgcgcatca
     4681 tcgccatgct gcgcaatcca gtcgacatga tccagtcgct ccatgcccac aagctggccg
     4741 gcgggaccga ggatctggcc agcctggaag aggcgctcga cgccgaggcc gatcgagccg
     4801 ccgggcgccg gctgcccgag ttctccaatc cgaagctggc gacgtaccgc gaccgggcgc
     4861 gctacggcga gcagctgccg cgctggttcg acgagttcgg ccgcgaccgc gtccgcgtca
     4921 tcatcttcga ggacatggtc agggacacgc cgggcgagtt ccgcgcgctg ctcgagttcc
     4981 tgggcgtcga tcccacgtat gcgccggagt ccttcatggc ccacaacgca gcccacggaa
     5041 cgcgtagccc tctcgtccgg agattggtgc gcgggcgcgt cgcccagtgg ctcgcctggg
     5101 gggtgctgcc gcgcatcatc ggcgcgacac ggacgaggag catggcccag cgcttcggcc
     5161 actcgcggat gaaccggagg acggttccga gaggcaagct gacgcctgag ctgcggcgcc
     5221 gtctcgagga cgatttcacg cccgacgtcg cgcggctgtc gagcctgctt gggcgcgaca
     5281 tgtcccagct gtggttcggc cggccgatca gcggctctgc tggcgcggtg ccagagccgg
     5341 ccgcggccca cgcggtcagt gcagattgag tcacggcgcc tgtctctggt tcaccgggct
     5401 gtccggctcg ggcaagacga cgaccgctga ggcgctcatc ccgctgctcg agcggcgcgg
     5461 ccggatcgtg accatgctcg acggcgatgt cgtgcgcacc cacctgtcgc gcggccttgg
     5521 cttctcacgc gaggatcgtg acgagaacgt gcgccgcgtc ggcttcgtcg cggccgagat
     5581 cgcgcgccac ggcggcctcg tcgtgtgcgc cctggtcagc ccctaccgcg cgacgcgcgc
     5641 tgaggtgcgg gcgatggtcg acgccgccgc aggcccggga gcgttcatcg agatccacgt
     5701 cgacacgccg ctggaagagg tcatggcgcg cgacgtcaag ggcctgtacg cggccgggac
     5761 gcagcagatg accggcttgt cggaccccta tgagccgccg ctcacgccgg agctgcggct
     5821 cgagaccgtc ggccgctcgg tcggccagaa cgcggcgcag gtgctcgacc tgctcgaaga
     5881 gcgcgggctg ctgtaagcct cagggcagca ggcgcggcgg gtcggtggtc aggtcgtacg
     5941 ccgcgatgca ttgcttgccg ggtgggcagt cgaacaggcg cgtatagcca tcggcgcgga
     6001 gctgcggcag cagctcgccc agggctcgtt ccagctggtc ctgctcgttg ccttcgagca
     6061 tcaggcccca acgctgcgcg tagtagaagg tggccggatt ccagccacgc ccgaccatga
     6121 cgatcatgtc cccgggcgcg gagcgctggt cgatgtattc cgcggcctcg aggtggtgct
     6181 cgccgaggtg cgtgtcgtag aactggatga cccagtaatg ctcagacgtc ttgaggttgc
     6241 cgatccagca tacgaccagg aatgcgccga gcagcggcgg caggatccat ttccacttgc
     6301 gcatcgtcca cacgagcccg atgcccacga ccgcggcggc catgggcgtc agcgcgatca
     6361 ggtagtagtc gtggatgtag tagaggttga cgaagaccag cgggccgatg aatgcgccgg
     6421 cgagccacgc aatcgtgaac agccgccgct tcaaccacag gctggcgagg aagccgagcg
     6481 ggatccacgc gaagccaatg gcgaatggca cgaagtaggt ctggccgccc agcaccagct
     6541 cgttgactcg acccaggatg agcccccagg tgtccggatt caggcgctgg tcgatcgagc
     6601 cgaagttcca cgtgaccagc gccgcgctcg tctggttggc cgtgaactcg gacgcggcct
     6661 tgaccttgtc ggcatagacg gtccatagcc cgccgaacag gagcggcacg gcgatcagcg
     6721 ccagcagcgc gccggcgtag agcagcggct tgcccagggg gcgttcgcgg ccggcccgga
     6781 agcctgtcca ggcgagcgcg aggatggccg gcaggtacat caccgcggtg gtgggcttga
     6841 ccatcatcgc gatcacgccc gcgcccaggg ccagggccca ccatgcccag tgacgcgact
     6901 cgtgccagcg cagcgcggcg tagaccgtgg tcagcgacgc ggcgacgacg aagtactcga
     6961 tcatcgacgt tcgcgcccag agcagggcca acgcgcacac cgcgaacgcg agcatggcga
     7021 cgagagcggc ggcctcgccg gcgatgcgcg cgacgaggcg cgacagcacg agcatggtgg
     7081 ccaggaagca cgccatgccg gtcacccgca tcgcgacatc aggctccacg ccggcattca
     7141 tgaccagcgc cccaagcgcc tggaagagtg gaaactcgaa cggcacgtcc cacggcggcc
     7201 cgaacaccgg gatcttggga tggagcaggt cgatcccctg ctcgtggaag atcagcgcag
     7261 tccaggcg
//
LOCUS       DATBRX010000241         6755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_3015739, whole genome shotgun sequence.
ACCESSION   DATBRX010000241 DATBRX010000000
VERSION     DATBRX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3015739"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VIF08_09050"
     CDS             complement(<1..438)
                     /locus_tag="VIF08_09050"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEY8136172.1"
                     /translation="MTNRVRSDEELAAVRIGPTEILNAQIYLAPYDKGWPRLFEREAD
                     RIRRALGTGAIVLEHAGSTSIPGLAAKPRIDIILGVADSADETGYVPPLDAAGYVLRI
                     REPDWHEHRVFKGPDTDVNLHVFSVGCAEIARMLAFRDHLRANG"
     gene            complement(442..972)
                     /locus_tag="VIF08_09055"
     CDS             complement(442..972)
                     /locus_tag="VIF08_09055"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8136173.1"
                     /translation="MRRWRRTRDLTLAQVAEGSSLNVGYLSQIENCKAVPSLDALLSI
                     ARALDVPAAWLLVDSSPPPRVVRAAERPAMADSLGGHATEVDGGTARDLRIIETVVPP
                     GMSTGVHAHTGDEHHLVLNGRFRMTQGEHVTELGPGDYLAWDPTIPHDVENIGKEPGR
                     LLLIYPRHGRRPDSSS"
     gene            1137..1781
                     /locus_tag="VIF08_09060"
     CDS             1137..1781
                     /locus_tag="VIF08_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8136174.1"
                     /translation="MKVAIIGSGNIGQALGGALVRAGHDVTLVARDAIATQNVANQIG
                     ATTAATAPEALGGSQVAVLAVPYTSLAEVAAGIKSVIAGKVVIDVSNPIGQAPAGTSA
                     AQQLAEWLPGARVVKAFNTTFAGLLATPEAHGQVLDALYATDDVDAGETVAELIRSIG
                     FRPVYAGGLSAAAQLEAMAGLNIQLQIATKGDWRSSFVLIGAPKGALEGLPKAA"
     gene            complement(1871..2284)
                     /gene="erpA"
                     /locus_tag="VIF08_09065"
     CDS             complement(1871..2284)
                     /gene="erpA"
                     /locus_tag="VIF08_09065"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY8136175.1"
                     /translation="MLNTEPQVNPQAPVQSEPLVVLSDAAASKLQELVTAEQNPNMGL
                     RVYVYSGGCSGFRYGMMLEDQPSSEDVTVESQGIKVYVDRNSTQYLTGSEIDYLDTLM
                     GAGFTVNNPNAVSACGCGSSFRTEESAGSPGACHH"
     gene            complement(2315..3829)
                     /locus_tag="VIF08_09070"
     CDS             complement(2315..3829)
                     /locus_tag="VIF08_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136176.1"
                     /translation="MAPLVADVDATAGAWHEWTDSLAGGYAPDANGNGSGYVENAAGG
                     ANGFLYAATSSLEPMIAAAQSDQPRAGDEPRPDAGDEPRPRPEARADAYDLPPATSHG
                     PVTPEYPWASISQHDTPPAPAAPWEEAEAYREAQAEVVAELTDTRVPAAAPEAEPAPA
                     EWAASPEPEPAPAEWAASPEPEPAPAEWAASPEPEPEPAPAGWAASPEPEPEPAPTPV
                     AHVADLQLPVQLSANQQNIVLRIELAIVDESRRPDAVHVVRRVGQDGDVLDLEYEPRD
                     GGRGLAPWPEYIWDTPPEMTRLRRREPGEQPHPDVAPAVDRQSARPTSQPMTLDWELP
                     AVGPAQSMPDPRAPSAPTVQPVAPYVPAPPQHELPTQPKHPPAAQLPYPPADPLYGLP
                     AAPNPQPARYSPPGYQPAPYSPSMPTQGQYPVPAVQSAPAAGARAGQVNAAADRADLW
                     FLSTQPAAATAADEEPGHANRPRTLMTVGLTIGVALLVLVLMLVFIQLMTSLLR"
     gene            complement(4222..5463)
                     /locus_tag="VIF08_09075"
     CDS             complement(4222..5463)
                     /locus_tag="VIF08_09075"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY8136177.1"
                     /translation="MTRDLRRPVREAWIVSAVRTPVGRYGGALARVRPDDLAATVIKA
                     AVERSGVRPDAIDDVIMGAANQAGEDNRNVARMSALLAGLPVGVGGQTVNRLCGSGLQ
                     AINTAAHAIMVGDGDAFVAGGVESMTRAPYVMAKAEAPWERGAREMFDTTLGWRFTNP
                     RLAEMYHPYTMGETGENVASQNKVSREDQDAFALVSHQRAVAAIEGGRFQNQIVPVMA
                     PQAKGEPLEISRDEHPRADTSMEALGKLRAAFREGGSVTAGNSSGINDGASATVVVAS
                     DFARAEGLKPLARVVATAVAGVDPSVMGLGPIPATRKALDRAGLRVEDLDLIELNEAF
                     ASQSIAVMRELALDPEKVNVNGGAIALGHPLGMSGARLITMLSHELARTGKRYGLATM
                     CIGVGQGIATIIERLDAEAVA"
     gene            complement(5544..>6755)
                     /locus_tag="VIF08_09080"
     CDS             complement(5544..>6755)
                     /locus_tag="VIF08_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8136178.1"
                     /translation="RLGAELAAGADHFSGAMIVGVVGAGTMGAGIAQVCLQAGHEVLL
                     HDVDHAAIERGRGRIADGLEKLVTKGRLTADQRTEMLASLRDAHSLEGLAQEVDVVIE
                     AALEDIDLKRTIFRALGASARPGAILATNTSALSVSEIGQSSGVPNRVVGLHFFNPAP
                     LMPLVEVVVGRQTDPATVDKATDFATGLGKEAVACADSPGFIVNRVNRPFTLEALRML
                     DAGEAGVEQIDRAVQSAGYPMGPFAFMDLVGIDVNLAAATAVWQGFDEAARFEPSRIQ
                     EELVAAGRLGRKSGEGFYVYKDGRPTGLSDRLPAGAQPAPDALDDAAIVARIELAIIN
                     EAYHAAGEGVAQPPDIDRALKLGASHPYGPFERAAVLGLRQVVTGLRDLEQRYGERFR
                     VAAALWQIASI"
BASE COUNT         1038 a         2234 c         2387 g         1096 t
ORIGIN      
        1 cccgttcgcg cgcaggtgat cgcgaaacgc cagcatgcgc gcgatctcgg cacagccgac
       61 gctgaaaaca tgcaggttga catcggtgtc cgggcccttg aaaacgcggt gttcatgcca
      121 atcgggctcg cggatgcgca gcacgtaccc cgccgcgtca agcggcggaa cgtatccggt
      181 ctcgtcggcg gagtcggcga ctcccaggat gatgtcgata cgtggcttgg ccgcgagccc
      241 gggaatggac gtcgacccgg cgtgctccag gacgatggcc cctgtgccga gcgctcgcct
      301 gattcgatcc gcctcgcgct cgaagagccg gggccacccc ttgtcgtacg gcgctaggta
      361 gatctgggcg ttcaggatct cggtgggacc tattcggacc gcggccagct cctcatcgct
      421 acgaaccctg ttcgtcatcg actagctcga agaatccgga cggcggccgt ggcgcggata
      481 gatcagcagc aaccggcccg gctccttacc gatgttctcg acatcatgcg gaatggtggg
      541 atcccaggcg aggtagtcgc ccggaccaag ctcggtcacg tgctcgccct gggtcatgcg
      601 gaagcggccg ttcaacacga gatggtgctc gtcaccggtg tgggcgtgca cgccggtgct
      661 catcccgggc ggcacgaccg tctcgatgat gcgcaggtcg cgcgccgtcc caccgtcgac
      721 ctccgtggca tgtccaccca ggctgtcggc catcgccggc cgctcggcgg cgcggacgac
      781 gcgtggcggg ggtgaggaat caacgagcaa ccaggccgcg ggcacatcca gcgcgcgcgc
      841 gatggacagc agcgcgtcca gcgacggcac ggccttgcag ttctcgatct gcgagagata
      901 gcccacgttg aggctgctcc cctcagccac ctgggcaagc gtcagatccc gcgttcgacg
      961 ccatcggcgc acctctttgc cgacagatga gctgccggcg atgcccgatt cttgtttctc
     1021 tttagacaac ccagttgact cccagcaata tcggtgtcat agtttacccg taagcaatat
     1081 aacttggccg acagcaaaga gcgagtcgga ccggggcaac agcaggagat ccattgatga
     1141 aggtagcaat catcggcagc ggcaacatcg gccaggcgct tggtggcgcg ctggtgagag
     1201 caggccacga cgtgacgctc gtggcgcgcg acgcaattgc gacgcagaac gtcgccaatc
     1261 agatcggcgc gacaacggcc gcgaccgccc cagaggcgct cggcggctcc caggtcgcag
     1321 tgctcgcggt gccatacacg tcgctcgcag aagtcgcggc cggtatcaag agcgtcatcg
     1381 ccggcaaggt cgtcatcgat gtgagcaatc cgattggcca ggcaccggcc ggcacttcgg
     1441 ccgcccagca acttgccgag tggctgcccg gcgcacgcgt cgtcaaggca tttaacacga
     1501 ccttcgcggg tctgctcgcc acgcctgagg cgcacggcca ggtccttgac gcgctgtacg
     1561 cgaccgacga tgtggacgcc ggcgagaccg tcgccgaact catccgttcg atcggcttcc
     1621 gcccggtcta cgcaggtgga ctcagcgcgg ccgcgcaact cgaggcgatg gccggtttga
     1681 acatccagct ccagatcgcc accaagggag actggcgctc cagtttcgtc ctaatcgggg
     1741 cgccgaaggg cgcgttggaa gggctgccca aggcggcttg atcaaggccg gacaggtcgg
     1801 ttggccctgg ccaggaatca caaacggcga gcctcgcggc tcgccgttct tgttctagtg
     1861 gggcgccgga tcagtggtgg caggcgcccg gtgagccggc cgactcctcg gttcggaagc
     1921 tagagccaca gccacaagcc gatacggcgt tggggttgtt gaccgtgaag cccgcgccca
     1981 tgagcgtgtc taggtagtcg atctccgacc cggtcaggta ctgggtggag ttgcggtcga
     2041 cgtagacctt gatgccctgg ctctcgacgg tgacatcttc gctgctcggc tgatcctcga
     2101 gcatcatccc gtagcggaag ccgctgcagc cgccgctgta cacgtacacg cgcagaccca
     2161 tgttcgggtt ctgctcggcg gtgacgagct cctgcagctt gctggcggcc gcgtcactca
     2221 gcacgacgag cggctctgac tggacagggg cttgcgggtt gacctggggt tccgtgttca
     2281 gcatgtgcga aagcctacca ctgagggtaa atgtttagcg cagcagtgaa gtcatgagct
     2341 ggatgaacac gagcatgagc acgaggacca gcagcgcgac gccgatcgtc agaccgactg
     2401 tcatcagggt ccgcggccga ttcgcgtggc cgggttcttc gtcggccgcc gtggcggcgg
     2461 cgggctgagt agagaggaac cacaggtcgg cccgatcggc ggcagcgtta acctggccgg
     2521 cgcgcgcgcc ggctgcgggg gccgactgga ccgcgggaac cggatattgg ccttgggtgg
     2581 gcatgctcgg cgagtacggc gctggctgat agccgggagg cgagtagcgg gcaggctgcg
     2641 ggttgggcgc ggccggaagt ccgtacagtg ggtcggcggg cgggtacggc agttgggcgg
     2701 cgggcggatg tttgggctgc gtcggcagct cgtgctgcgg cggtgccgga acatagggcg
     2761 ccactggctg cactgttgga gccgacggcg cgcgtggatc aggcatggac tgagcaggcc
     2821 cgacagcagg cagctcccag tccaaggtca tcggctgcga tgtgggccga gcggattgcc
     2881 gatccaccgc gggggcgaca tcgggatgcg gctgctcgcc aggctcgcgc cgtcggagac
     2941 gggtcatttc cggcggtgtg tcccagatgt actccggcca tggcgccaaa ccgcgtccgc
     3001 catcgcgcgg ctcgtactca agatcgagca cgtcgccgtc ctgtccgacg cgtctgacga
     3061 catgcacggc gtcggggcga cggctctcgt caacgatcgc cagctcgatg cgcagcacga
     3121 tgttctgttg gttcgccgac aactggactg gcagttgcag atcggcgacg tgtgccacag
     3181 gggtcggcgc gggctcgggt tcgggttcgg gcgatgcggc ccacccggca ggcgcgggct
     3241 cgggttcggg ttcgggcgat gcggcccact cggcaggcgc gggctccggc tcgggcgatg
     3301 cggcccactc ggcaggcgcg ggctccggct cgggcgatgc ggcccactcg gcaggcgcgg
     3361 gctcggcctc gggcgcggcc gcgggcacgc gcgtgtcggt cagctccgct accacctcgg
     3421 cctgcgcctc ccggtacgcc tccgcctcct cccatggcgc ggctggcgcg gggggagtgt
     3481 catgctggga gatggaggcc cacgggtact ccggcgtgac ggggccgtgg ctcgtggcgg
     3541 gcggcaggtc ataggcgtct gcccgggctt cagggcgcgg gcgcggctca tctccggcgt
     3601 cagggcgcgg ctcgtctccg gcgcgcggct gatcgctctg cgcggcggcg atcattggtt
     3661 caagcgagct cgtggcagcg tagaggaagc cattggcgcc gccagcagcg ttctcgacgt
     3721 agccgctgcc attgccgttg gcatcgggtg cgtagccccc tgcaagactg tctgtccatt
     3781 cgtgccacgc acccgccgta gcgtccacgt cagcgacgag gggcgccatg gcgtcatccg
     3841 ggtcctgtga ctggtcgctc gcggggcggt cccaaggcgc agcgtcaccc aaaggatcgg
     3901 ccgcgggcca ggcaggcagc acaggttccg gcgccggagg agctggctgt gccaaccagg
     3961 tgtgcgagta gtcggtgcca gagtcctctt cgggcggcca ctcctcggga gccggccgat
     4021 cccatggaac gtcggcgcga gcctgtggat ccgggggcgc gaagggcggg tcctgctgtg
     4081 ggtgcgccgg ttccgcgggt gcgaacgagc cgtcgtcggc caatgggacg tcccacggcg
     4141 agggcggctg agcaggcgac cttctcggcg gcgtgggctg ggagggcggt cggagccggc
     4201 gcgccatgag gtcgttatcc atcaggccac cgcctccgcg tcaaggcgct cgatgatcgt
     4261 ggcgataccc tggcccacgc cgatgcacat cgtcgcgagg ccgtagcgct tgccggttcg
     4321 ggcgagctcg tggctcagca tggtgatcag tcgcgcgccg ctcatgccca gcggatgacc
     4381 cagtgcgatg gcgccaccat tgacattgac cttctcgggg tcaagggcga gctcgcgcat
     4441 gacggcgatg ctctggctgg caaacgcttc attcaattcg atcagatcga ggtcctcgac
     4501 acgcaggccg gcgcggtcga gtgccttgcg cgtggccggg attgggccca ggcccatcac
     4561 tgacgggtcg acgccggcga cggccgtcgc gacgacgcgc gcgagcggct tcaggccttc
     4621 cgcacgggcg aagtcactgg ccacgaccac tgttgccgac gcgccatcgt tgatgccgct
     4681 cgaatttccg gcggtgacgc tgccgccctc gcggaacgcg gcgcgcagtt tgcccagcgc
     4741 ctccatcgac gtgtcggcgc gcgggtgctc gtcgcggctg atctcgagtg gctcgccctt
     4801 ggcctggggc gccatcacgg gcacgatctg gttctggaaa cgcccgccct cgatggccgc
     4861 gacggcgcgc tggtgggaca cgagcgcaaa cgcgtcctgg tcctcgcggc tgaccttgtt
     4921 ctgcgacgcg acgttctcgc cggtctcgcc catggtgtac gggtggtaca tctcggccag
     4981 ccttgggttc gtgaaacgcc agccgagcgt cgtgtcgaac atctcgcgcg ccccgcgctc
     5041 ccacggcgcc tcagccttgg ccatgacata cggtgcgcgg gtcatcgact cgacgccccc
     5101 ggcaacgaac gcatcgccgt cgccgaccat gatcgcgtgc gccgccgtgt tgattgcctg
     5161 caggccactg ccgcacagcc gattgaccgt ctggccaccg actcccaccg gcagcccagc
     5221 caacagcgcg ctcatccggg ccacgttgcg gttgtcttcg cctgcttgat tggccgcgcc
     5281 catgatcacg tcatcgatag cgtcggggcg cacgccggag cgctcgaccg ccgccttgat
     5341 gacagtcgcg gcgaggtcgt cgggccgcac gcgggcaagc gcgccgccat accgcccaac
     5401 gggcgtgcga acagcgctca cgatccatgc ttcgcgcacg ggccggcgaa ggtcccgagt
     5461 catgggggga ttgtaggagc ggtgcctacc agcaccggca tagcccaaag gtcagggcgg
     5521 ttaggaccgg ttgtgtaccg cgatcagatg gaggcgatct gccagagggc ggcggcgacg
     5581 cggaatcgct cgccgtaacg ctgctcgagg tcgcgcagcc cggtcacgac ctggcgtagg
     5641 ccaaggaccg cggcgcgctc gaacggaccg tacggatggc tggcgccgag cttcagcgcg
     5701 cggtcgatat ccggcggctg ggcgacgccc tccccggccg cgtggtacgc ctcgttgatg
     5761 atcgccagct caatgcgcgc aacgatcgca gcgtcgtcca gcgcatccgg ggcgggctgg
     5821 gcaccagctg gcagtcggtc agacagtccg gtcggtcggc cgtccttgta aacgtagaag
     5881 ccctcgcccg acttgcgccc aagacggccc gctgccacca gctcctcttg gatgcgcgac
     5941 ggctcgaagc gggccgcctc gtcgaaaccc tgccacaccg cggtcgccgc ggcgaggttg
     6001 acgtcgatgc cgacgagatc catgaaagcg aacgggccca tgggatagcc ggccgactgg
     6061 accgcgcggt cgatctgctc cacgcccgcc tcacccgcgt cgagcatgcg cagggcctcg
     6121 agcgtgaagg gccgattgac ccggttgacg atgaagccgg gcgaatcggc gcaggcaacg
     6181 gcctccttgc cgaggcccgt cgcgaagtct gtggctttgt cgaccgtggc tgggtcggtc
     6241 tggcggccca cgaccacttc gacgagcggc atcagcggcg ccgggttgaa gaagtgcaga
     6301 ccgacaactc ggttgggcac gccgctcgac tggccgatct ccgacacgga cagcgcactg
     6361 gtgttggtgg ccaggattgc tcccggtcgg gctgaggcgc ccagggcgcg gaagatcgtg
     6421 cgcttgaggt cgatgtcctc gagcgcggcc tcgatgacga cgtcaacttc ctgggccagc
     6481 ccttccagcg agtgcgcgtc gcgcaggctg gccaacatct ccgtacgctg gtcggcggtg
     6541 agtcggcctt tcgttacgag cttctcgagg ccgtcggcga tgcggccgcg gccgcgctcg
     6601 atcgcggcgt ggtcaacgtc gtgcagcagc acctcgtgcc cagcctgaag acagacctgg
     6661 gcgatccccg cgcccatcgt gccggccccg acgacgccaa cgatcatggc gccagagaag
     6721 tgatcggctc cagcagcaag ctctgctccg aggcg
//
LOCUS       DATBRX010000242         8942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U4140 k141_1451471, whole genome shotgun sequence.
ACCESSION   DATBRX010000242 DATBRX010000000
VERSION     DATBRX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959040
            Sequence Read Archive: SRR11673297
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:03:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,824
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451471"
                     /isolate="SMAG_U4140"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VIF08_09085"
     CDS             <1..1324
                     /locus_tag="VIF08_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY8136179.1"
                     /translation="ADGEPPEWITDEARASAERRGRGRVPLISISGTNGKSTTTRMIS
                     HIARLAGRHVGTTTTDGVLIDERIVEPGDYTGPAGARAVLNHPEVDLAVLETARGGIL
                     LRGLGYESNDVSVLTNVSGDHLDLQGLHTLPELAEVKSVICRVTAPTGTVILNADDPL
                     VAAVSTRVRATVVLFSERNSGSRLRRHLARGGRAFIHEAGWVVELAGERRRQIVPAAE
                     IPATLGALARHNVANALAAAAGARALGFSRSQVAAGLRDFHISPELMPGRLNFYRRGS
                     RLVVVDYAHNVAGLTVLLDTVEALIGRRGRRRATLSLIVGSAGDRPDDALRELGELAA
                     RRADELALKEDLPFLRGRSRESVIGELRAGFRAGGAKAKKVALYIDEAAALRGELETV
                     GRLGADESGPPRVVVLMCHAHREEVAEYLAKAGFEPANDVAALSDFRA"
     gene            complement(1321..1767)
                     /locus_tag="VIF08_09090"
     CDS             complement(1321..1767)
                     /locus_tag="VIF08_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8136180.1"
                     /translation="MRLRILRLTLDRALTNKEMAERLGRDPGTVLHHVRVLVRGGFLE
                     AGKVRPGKRGALERPYRATGKSWRIRMRQGSDYTASVLDAVRDEIEESDERAALSMVR
                     LGVRLTDADIEDLRHRVGELGDEFVLRDDPAGTPIGIMALVYKRRP"
     gene            2094..2939
                     /locus_tag="VIF08_09095"
     CDS             2094..2939
                     /locus_tag="VIF08_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136181.1"
                     /translation="MLIEVQESPIGIAFGRDTIWVAHYFAGEIFMIDPVSHSITGAVD
                     VGVGAIGLAAGPDALWVSVHESGELVRIAYEDESLTRIAVGPQPEGVAAAGDQVWVTS
                     EEDDQVIRVDPQTNEVVATVDVGQEPRHVGLGFGSVWVTNFGSRSISRIDPATNQVSA
                     TIRGEYGPEGIGFTSNAVWVANVIDGSVSRIDPATNELVTTIPTGYATEGIVVDPDGL
                     VYAAVTGAIGIAGIDPQTNEVVAVFSTGNQPRRMVILGEELWVTNTGDGTVQILDLNN
                     PRTPG"
     gene            complement(2936..5659)
                     /gene="cphA"
                     /locus_tag="VIF08_09100"
     CDS             complement(2936..5659)
                     /gene="cphA"
                     /locus_tag="VIF08_09100"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY8136182.1"
                     /translation="MRILETRVLRGPNIWWRAPAITLLVDLGELEDWPSNTIPGFNEA
                     LIALIPSLEDHACSLGRRGGFVSRLKDGTWLGHVTEHVAIELQSMAGTEAHLGKTRSA
                     GGQGRYNVVYEYRDEDVGKEAGRLAVALVNHLVAPEAAPLDFAEELEDLIRLAERRAF
                     GPSTQALVDEALSRDIPYIRLDHHSLVQLGQGVHQQRIRATMTSRTPAIAVDMASDKS
                     LTNALLSSAGLPVPRSRVVRTVDGAVEAAHEIGFPCVVKPLDGNHGRGVGLNLRSDAD
                     VGKAFKPAYEESNRGDVVVESFVKGNDYRVLIVGGKMAACAERVPASVVGDGKLTVAQ
                     LVERENADPRRGIGHEKVLTRIKVTPAAEELVAKQGYAMDAVPPKGTRVKLASTGNMS
                     TGGTSIDRTEDAHPDNVEIAETAARVIGLDVAGIDFIAPDIAVPVREQGGAIVEVNAA
                     PGFRMHTHPTEGEPQYVAKPVIDLLFAPGSNARIPIVGVTGTNGKTTTVRMIGHILKL
                     MGRRVGMTTTDGIVVDGRLIRKGDMSGPKSAQMILQNPSVDTAVFEVARGGILREGLG
                     YDRNDVAVVTNVTGDHLGLRGVETMGQLAHVKSVLVDAVPRSGSAVLNADDELVSRMA
                     RQCRGHVVYFSMARERSEPGFDRVDGHCGRGGAAMVLQQSDEGELVVLRHADRTMPLL
                     YTHLIPATFGGRARMNVANALAAAAAAWASGAHLHDIRQGLRTFSTSFFQAPGRLNEI
                     EVDGYKVFLDYCHNVDGMRRLAEFVDAMAHGKGATPGARAIGVIGIPGDRRDEDHHEY
                     GRLAGLVFDSIVVREDKNLRGRKPGESAALVLAGVKAAQKQKGRVQEATIVTNEQEAA
                     ATGMGLAVPSDLVVLCSDDIQAVYRRVMAESKSRVGGAAIGAPGEFAVDEG"
     gene            complement(5760..6596)
                     /locus_tag="VIF08_09105"
     CDS             complement(5760..6596)
                     /locus_tag="VIF08_09105"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEY8136183.1"
                     /translation="MTEGHVIIIGGAEDKVRERVILSRFVTLAGGRNARIAVISTASS
                     LGPLAGQLYRKVFGELGAAEVTPIHTTNRAQCSSESAIRAVEDATGVFLTGGNQLRLS
                     STIGGTPLAQAVVRRHREGAVVAGTSAGASAMSTHMVAFGASGATPKQRMVHMAAGLG
                     LLPGVIVDQHFEARNRLGRLLAIIAQNPSLLGIGVDEDTAGVVGPDRVLEVVGRRSIT
                     IIDGSESETDAWEVKAHRPMMISNVKLHSLSNGYRFNLDTRERVEAPMLHAVDIEAIA
                     SS"
     gene            6706..7536
                     /locus_tag="VIF08_09110"
     CDS             6706..7536
                     /locus_tag="VIF08_09110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY8136184.1"
                     /translation="MSSRLRLEPRRLLAIALLALTGGIVLAFLWARGERAGSDAFAYW
                     TAVQRWLSGQDIYHVTPGLDLPPSEGALPFAYAPWSLYLFLPSAVLPWGMFWFAWRAA
                     NIALFAWSVAWAYERRPLGTAVFVALLGPALAANLDTGNVNVLIVLGVWLAWFVGPRL
                     GGTAWAVGAALKFLPFVLLAFVPRRAWRPGLVAFGVLIVLTLASWPQVVRQLQIVLDY
                     PRPLRIDYMLLAWAAVPWLWARSWPPPLKGLRRWLTSEPQAAPPRYDPAGVSADLGKG
                     "
     gene            complement(7494..8327)
                     /locus_tag="VIF08_09115"
     CDS             complement(7494..8327)
                     /locus_tag="VIF08_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY8136185.1"
                     /translation="MPEGDTLARIAHALRPVLAGKRIVAARGRPGGARLELVIGATVS
                     SVEARGKHLLIGFDSGLTLHTHLGMNGSWHRYRADERWRRPAARAVAVLTTRDATAVC
                     FDAPTVELLDTRALAAHPVLRAMGSDIATADFDLDAALAALREPRRAAMAVGDALIDQ
                     SALAGLGNVYRSELPFIERVNPLTRVSDVTAMQLRAMLERGAVLVRANSAGGARVTTP
                     AGTPGNVHVYGRTGRPCPRCGSRIVSRATRARPESSPRRAYWCPSCQPLPRSAETPAG
                     S"
     gene            complement(8340..>8942)
                     /locus_tag="VIF08_09120"
     CDS             complement(8340..>8942)
                     /locus_tag="VIF08_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8136186.1"
                     /translation="LRLLDAYGVVTRDSVAAESLPGGFSAVYPVLRELEERGRVRRGY
                     FVEGLGGAQFCQPAALDRLRAERSDAGGDRRAAETLLLAASDPANPYGATLAWPRWSD
                     EDRRPLARAAGAYVVLVGGEAALYLERGGKSLQTLPSFVDADVAATALNALRQLLADG
                     RVRALQIERVDGAPVSESPHAATLAAAGFERAYRGWLLRA"
BASE COUNT         1280 a         3143 c         3128 g         1391 t
ORIGIN      
        1 tgcggatggg gagccgcccg aatggatcac cgacgaggcg cgggccagcg ctgagcggcg
       61 cggtcgcggt cgggtgccgc tcatcagcat cagcggcacc aacggcaaga gcacgaccac
      121 gcgcatgatc agccacatcg cgcgccttgc cggaaggcat gtcggcacga ccacgacaga
      181 cggcgtgttg atcgacgagc gcatcgtcga accgggcgac tacaccgggc cggccggcgc
      241 cagggcggtg ctgaaccatc ctgaggtcga cctcgcggtg ctcgagacgg cgcgcggcgg
      301 gatcctgttg cgcggccttg gctacgagtc caacgacgtg tccgtcctga ccaacgtcag
      361 cggcgaccac ctcgacctgc agggcctgca caccctgccg gagctggccg aagtcaagtc
      421 ggtcatctgc cgcgtgaccg cacccacagg gacggtcatc ctgaacgccg acgacccgct
      481 cgtcgccgcg gtcagcacgc gggtgcgggc aacggtcgtg ctgttcagcg agcgcaactc
      541 agggtcgcgg ctgcgccgcc acctggcacg cggcggacgc gccttcatcc acgaggccgg
      601 ctgggtcgtc gagctggccg gtgagcgacg gcggcagatc gtgccggcgg cagagatccc
      661 ggcaacactg ggcgctcttg cgcggcacaa cgtggccaac gcgcttgcgg ccgccgccgg
      721 cgcacgtgcc ctgggcttca gccgctcgca ggtcgcggcc gggttgcgcg acttccacat
      781 ctcacctgag ttgatgccgg gacggctcaa cttctaccgc cgcggcagcc ggctggtcgt
      841 cgtcgactac gcccacaacg tcgccggctt gaccgtgctg ctcgacacgg tcgaggcgct
      901 cattggccgg cgcggccgac ggcgcgcgac gctgtcactg atcgtgggca gcgccgggga
      961 ccggccggac gacgcgctac gagaactggg cgagctggcc gcgcgccggg ccgacgagct
     1021 ggcgctcaag gaggacctgc cgttcctgcg cggacgatcg cgcgagagcg tcatcggcga
     1081 gctgcgcgct ggcttccgcg cggggggcgc caaagccaag aaggtcgcgc tctacatcga
     1141 cgaggcggcc gcgttacgcg gcgagctgga gactgtgggc cggctgggag cggacgagag
     1201 cggtccgccg cgggtcgtgg tgctgatgtg ccacgcccac agagaagagg tcgccgaata
     1261 cctggccaag gccggcttcg agcccgccaa cgacgtggcg gcgctctccg acttccgcgc
     1321 ctagggccgg cgcttgtaga cgagcgccat gatgccgatc ggcgtgccgg ccgggtcgtc
     1381 gcgaagcacg aactcgtcgc ccagctcgcc gacacgatgg cgtagatctt cgatgtcggc
     1441 gtcggtcagg cgtaccccga gccggaccat cgatagcgcc gcccgttcat cgctctcctc
     1501 gatctcgtcg cggactgcgt ccaggaccga ggcggtgtag tcgctgccct ggcgcatgcg
     1561 gatgcgccac gacttcccgg tagcgcgata cggccgctcc agggcgccgc gcttgccggg
     1621 ccgaaccttg cccgcctcca ggaacccgcc gcggaccagg acccgcacgt ggtgcagcac
     1681 ggttccgggg tcgcggccca ggcgctcggc catctccttg ttggtcagcg cgcgatcgag
     1741 cgtcaggcgc aggatgcgca gccgcattgg attcgccagg gcgcgcatct ctgccggcgt
     1801 cgcgtcgcgt tcgggcgggg atgcgtgggt gccagtgcct aagctgagct ggggaggagt
     1861 gatggacagt tctccatcgg tgggttagag tccaccacat tatgcctgaa ccaccgatgc
     1921 tgcgggtcgg agttgctgca ctcctcgtgc tggcctgcgc cgcgccgggg gcgacagcca
     1981 cgcccgcgat attaccgaca gcccagccga cggccacgcc gaccgctgcg gctactgccc
     2041 ctcctccgcc gacaagctcg ccctcgccgt cggtcgcatc aacccctgag ccggtgctca
     2101 tcgaagtcca ggagtcgccg attggtattg ccttcggccg ggacacgatc tgggtcgccc
     2161 actacttcgc cggcgagatc ttcatgatcg accccgtctc ccattcgatc accggcgcgg
     2221 tggacgttgg tgtaggcgcc ataggtctcg cggcgggtcc cgacgcccta tgggtgagcg
     2281 ttcacgagag cggtgagctg gtgcgaatcg cctacgagga tgagtcgttg acgcgcatcg
     2341 cagttgggcc gcagccagaa ggcgtggccg cggccggtga tcaggtctgg gttacgagtg
     2401 aggaagacga ccaggtcatc cgtgtcgatc cgcagacgaa cgaggtcgtg gccacggtgg
     2461 acgttgggca ggagccgcgc cacgtcgggc tcggcttcgg ctctgtctgg gtcaccaact
     2521 tcggctcgcg ctccatttcg cgcatcgacc cggccaccaa ccaggtcagc gcgactatcc
     2581 ggggtgagta cggacccgag ggcatcggct tcaccagcaa cgcggtgtgg gtggccaacg
     2641 tcatcgacgg cagcgtgtca cgcatcgatc ccgcgacaaa cgaattggtg acgaccatcc
     2701 ccaccggcta cgccactgaa ggcatcgtgg tcgaccccga cgggctcgtc tacgccgcgg
     2761 tcaccggcgc cattggcatc gccggcatcg atccacagac caacgaggtc gtcgcggtct
     2821 tctcgacggg caatcagccg cggcggatgg tcatcctcgg cgaagagctg tgggtcacca
     2881 acaccggcga cggaacggtc cagatcctgg acctgaacaa tccgaggacc ccgggctagc
     2941 cctcgtcgac cgcgaactcg cccggcgcgc ctatcgcggc gccgccaacc cgggacttgg
     3001 actcggccat gacgcggcgg tagaccgcct ggatgtcgtc gctgcacagg accacgagat
     3061 cgctcggcac cgccaagccc atgcccgtgg ccgctgcttc ttgctcgttg gtcacgatcg
     3121 tcgcctcttg cacgcggccc ttctgcttct gcgccgcctt gacgccagcc agcacgaggg
     3181 cggccgattc gcccggcttg cggccgcgca ggttcttgtc ctcgcgcacg acgatcgagt
     3241 cgaagacgag tcccgcgagg cgcccgtact cgtggtggtc ctcgtcgcgc cggtcgccgg
     3301 gaatgccgat gacgccgatc gccctggcgc ccggtgtcgc gcccttgcca tgggccatcg
     3361 catcgacgaa ctcggccagg cggcgcatgc cgtccacgtt gtggcagtag tcgaggaaga
     3421 ccttgtagcc gtcgacctcg atctcgttga ggcggcccgg cgcctggaag aacgaggtgc
     3481 tgaaggtgcg caggccctgg cggatgtcgt gcagatgcgc gccgctcgcc cacgccgccg
     3541 cggcagccgc gagggcgttg gcgacgttca ttcgggcgcg gccaccgaag gtcgccggga
     3601 tgaggtgcgt gtagagcagc ggcatcgtcc ggtcggcatg acgcagcaca acgagctcgc
     3661 cctcgtcgct ctgctggagg accatcgccg cgccgccgcg gccacagtgc ccgtcaaccc
     3721 gatcgaagcc cggttccgag cgctcacgtg ccatggagaa gtagacgacg tggccgcggc
     3781 actggcgggc catgcgcgac acgagctcgt cgtcggcgtt caggaccgcg ctgccgctgc
     3841 gtggtaccgc gtcgaccagc acgctcttga cgtgcgccag ctggcccatg gtctcgacgc
     3901 cgcgcagccc gaggtggtcg ccggtcacgt tggtcaccac cgcgacatcg ttgcggtcgt
     3961 agcccaggcc ctcgcgcagg atgccgccgc gcgcgacctc gaaaaccgcg gtatcgaccg
     4021 acggattctg gaggatcatc tgcgccgact tcggcccgga catgtcgccc ttccggatga
     4081 gccggccgtc gacgacgatg ccgtcggtcg tggtcatgcc gacacgcctg cccatcagct
     4141 tcaggatgtg gccgatcatg cgcacggtcg tggtcttgcc gttggtgccg gtcacgccga
     4201 cgatggggat gcgcgcgttg ctgccgggcg cgaagagcag gtcgatgacc ggcttggcga
     4261 cgtactgcgg ctcgccctct gtggggtgcg tatgcatgcg gaagcccggc gccgcgttga
     4321 cctccacgat cgcgccgccc tgctcacgca ccggcaccgc tatgtccggc gcgatgaagt
     4381 cgatgcccgc cacgtccagc ccgatcaccc gggccgccgt ctcggcgatc tcgacgttgt
     4441 ctggatgggc gtcttcggtg cggtcgatgg acgtcccgcc ggtcgacatg ttgccggtcg
     4501 aggcgagctt gacgcgcgtc cccttgggcg gcacggcgtc catcgcgtaa ccctgcttcg
     4561 cgaccaactc ctccgccgcg ggcgtgacct tgatgcgcgt cagcaccttc tcgtggccga
     4621 tgccgcgccg cgggtcggcg ttctcacgtt cgaccagctg ggccacggtc agcttgccat
     4681 cgcccacgac gcttgccggg acgcgctccg cgcaggccgc catcttgccg ccgacgatca
     4741 gcacgcggta gtcgttgcct ttcacgaacg attcgaccac cacatcaccg cggttgctct
     4801 cctcgtacgc gggcttgaaa gccttgccca cgtcggcgtc gctacgaagg ttcaggccaa
     4861 cgccccggcc gtggttgcca tcgagcggct tgacgacgca cgggaagccg atctcgtgtg
     4921 ccgcctcgac cgcgccgtcc acggtgcgca cgacccgcga gcgcgggacg ggcagcccgg
     4981 ctgagctcag cagcgcattt gtgaggctct tgtctgaagc catgtcgacg gcgatcgcgg
     5041 gtgtgcgcga ggtcatcgtg gcgcggatgc gctgctggtg gacgccctgg cccagctgga
     5101 cgaggctgtg gtggtcgagt cggatgtagg ggatgtcgcg cgagagtgcc tcatccacca
     5161 gcgcctgggt cgatgggccg aaggcgcggc gctcggcgag ccggatcagg tcctcgagct
     5221 cttcagcgaa gtcgagcggt gctgcctcgg gcgcgacaag atggttgacc agcgccacgg
     5281 cgagccgccc ggcttccttg ccgacatcct cgtcgcgata ttcgtacacc acgttgtagc
     5341 gcccctgccc accggcggag cgggtcttgc ccaggtgggc ctccgtgccg gccatgctct
     5401 gcagctcgat cgcgacgtgc tcggtgacgt ggccgagcca ggtgccgtcc ttgaggcggc
     5461 tgacgaagcc gccccgccgg ccgagtgagc aggcgtgatc ttcgagcgac gggatgagcg
     5521 cgatcagcgc ctcgttgaag cccgggatcg tgttcgacgg ccagtcctcg agctcgccca
     5581 ggtcaacgag cagcgtgatc gcgggcgcgc gccaccagat gttggggccg cgcaggacgc
     5641 gcgtctcgag gatgcgcaac gtcggcgcgg gcccgcgacg ctggacggcg cgcgactttc
     5701 gggcgggcgc tgccctcttc gcgggcgccg gagccttagc cgcgcgtttg ggcgcggcct
     5761 tagctggacg cgattgcttc gatgtcgacg gcatggagca tgggggcttc gactcgctcg
     5821 cgagtgtcga gattgaagcg gtagccgttg gaaagcgaat gcagcttcac gttgctgatc
     5881 atcatcggcc ggtgggcctt gacctcccag gcgtcggtct ccgattccga cccatcgatg
     5941 atggtgatgg agcgccgacc gaccacctcg agcacccggt cgggtccgac cacccccgcg
     6001 gtgtcctcgt cgacgccaat gcccagcagc gatgggttct gggcgatgat cgcgagcagc
     6061 ctccccagcc ggttgcgagc ctcgaaatgc tggtccacga tcaccccggg cagcagcccg
     6121 aggccggcgg ccatgtggac catccgctgc ttgggtgtcg cgccacttgc gccgaatgcg
     6181 accatgtgcg tgctcatcgc ggaggcaccg gcgctcgtgc cagccacaac ggcgccttcg
     6241 cggtgacgcc gaaccaccgc ctgcgcgagg ggcgtgccgc cgatcgtgga tgacaagcgc
     6301 agctggttgc cgccggtcag gaagacgccc gtcgcatcct cgaccgcccg aatggcgctc
     6361 tcgctcgagc actgtgcccg gttggtggtg tgaatcgggg tcacctcggc cgcgcccagc
     6421 tcgccgaaga ccttgcggta cagctggccg gccagcgggc cgagcgacga ggccgtgctg
     6481 atgaccgcga tccgtgcgtt acggccaccc gccaaggtga caaaacgcga cagaatcacg
     6541 cgttcccgga ccttgtcttc cgcgccaccg atgatgatga cgtgaccctc tgtcattccg
     6601 cgggagtata ggatggtgcg cagggcgggg gcgcccaaca cgtttggtcg aggaggacga
     6661 acggagcccc gcccgcttcc ctctcccgtc tctatgctgc gcggcgtgag cagccgcctg
     6721 cgcctggagc cccgccgcct gctggccatc gcgctgctgg cgctgaccgg cggcatagtc
     6781 ctcgccttcc tgtgggcacg cggcgagcga gcgggttcgg acgcattcgc gtactggacc
     6841 gccgtgcagc gctggctgag cggccaggac atctaccacg tgacgccggg cctggacctg
     6901 ccgccgtcag agggcgccct gccgttcgcc tacgcgccgt ggtcgctcta cctcttcctg
     6961 ccctcagccg tgctgccgtg gggcatgttc tggttcgcct ggcgcgccgc gaacatcgcg
     7021 ctcttcgcgt ggtccgtggc gtgggcctac gagcgccggc cgctcggtac ggcggttttc
     7081 gtcgcactgc taggtccggc actggccgcc aacctggaca cgggcaacgt caacgtgctg
     7141 atcgtgctcg gggtgtggct ggcctggttc gtgggaccgc gcctgggcgg cacggcctgg
     7201 gccgtgggcg cggcgctcaa gttcctgccc tttgtgctgc tggcattcgt gccgcgccgc
     7261 gcgtggcgcc ctggcctcgt ggcgttcggg gtgctcatcg tgctgacgct ggcgagctgg
     7321 ccccaggtcg tgcgccagct tcagatcgtg ctcgactacc cacgcccgct gcgcatcgac
     7381 tacatgctcc tcgcgtgggc ggccgtgccg tggctgtggg cgcgctcatg gccgccgccg
     7441 ctcaagggtc tgcggcgctg gctcaccagc gagccacagg ccgcgccgcc gcgttacgac
     7501 cccgcagggg tttcggccga cctcggcaag ggttgacagg acgggcacca gtacgcccgg
     7561 cgcgggcttg attcgggcct tgcccgggtt gccctggaga cgattctcga gccgcaacgc
     7621 gggcacggcc ggcccgtccg accatagaca tggacgtttc ccggagtgcc ggccggcgtc
     7681 gtgacccggg cgccgccagc gctgttcgcg cgcaccagca cggcaccgcg ctcgagcatc
     7741 gcgcgcagct gcatggcggt gacatccgag acccgcgtga gcggattgac gcgctcgatg
     7801 aacggcagct cggagcggta gacgttgccc agcccggcaa gggccgactg gtcgatcagc
     7861 gcgtcgccca cggccatggc ggcgcgccgc ggctcgcgca gcgcggccag cgccgcgtcg
     7921 agatcgaagt cagcggtggc gatgtcgctg cccatggcgc gcaacaccgg atgtgccgcc
     7981 agcgcgcgtg tgtcgagcag ctcgaccgtg ggcgcgtcga agcagaccgc ggtggcgtcg
     8041 cgcgtcgtca gcacggcgac cgcgcgcgct gccggacgac gccagcgctc atcggcccgg
     8101 tagcgatgcc acgagccgtt catcccgagg tgcgtgtgca gcgtcaggcc gctgtcgaag
     8161 ccgatcagca ggtgcttgcc gcgcgcctcg acgctgctga cggtggcacc gatcaccagc
     8221 tcgagccgcg ctccgcccgg gcggccgcgc gcggcgacga tgcgcttgcc ggccaggacc
     8281 ggccgcagcg cgtgcgcgat ccgcgccagg gtgtcgccct cgggcatgcc tgtctccggc
     8341 taggcgcgga gcagccagcc ccgataggcg cgttcgaagc cggcggcggc gagcgtggcc
     8401 gcgtgcggcg attcgctgac gggtgcccca tcgacgcgct cgatctgcag ggcgcggacg
     8461 cggccatcgg ccagcagctg gcgcagcgca ttgagcgctg tcgcggcaac gtccgcgtcg
     8521 acaaacgaag gcagcgtctg gagcgacttc ccgccgcgct ccaggtacag cgccgcctca
     8581 ccgccgacca gcacgacgta cgcgccggcc gcacgggcca gcggtcggcg gtcctcgtcc
     8641 gaccaacgcg gccaggcgag cgtcgcgccg tacggattgg ccggatccga tgcggcgagc
     8701 agcaacgtct ctgcggcccg tcggtcaccg cccgcgtccg atcgctcggc gcgcaaccgg
     8761 tccagcgccg cgggctgaca gaactgagca ccgcccaggc cctcgacgaa gtagccgcgt
     8821 cggacgcggc cgcgctcctc caactcgcgc aggaccgggt agacagcaga gaagccgccg
     8881 ggcaggctct ccgccgcgac cgaatcgcgc gtcaccacgc catatgcgtc gagcaggcgc
     8941 aa
//
